LOCUS       DAJTVJ010000001        88078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000001 DAJTVJ010000000
VERSION     DAJTVJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88078
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.001"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO448_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO448_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6335340.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="OO448_000002"
     tRNA            1230..1305
                     /locus_tag="OO448_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="OO448_000003"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="OO448_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6335341.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="OO448_000004"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="OO448_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6335342.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="OO448_000005"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="OO448_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6335343.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="OO448_000006"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="OO448_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6335344.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="OO448_000007"
     CDS             complement(4868..5305)
                     /locus_tag="OO448_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR6335345.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="OO448_000008"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="OO448_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="OO448_000009"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="OO448_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCR6335346.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="OO448_000010"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="OO448_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCR6335347.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8268..9080
                     /gene="pduB"
                     /locus_tag="OO448_000011"
     CDS             8268..9080
                     /gene="pduB"
                     /locus_tag="OO448_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCR6335348.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="OO448_000012"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="OO448_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCR6335349.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="OO448_000013"
     CDS             10773..11441
                     /locus_tag="OO448_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCR6335350.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="OO448_000014"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="OO448_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCR6335351.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="OO448_000015"
     CDS             11984..13816
                     /locus_tag="OO448_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCR6335352.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="OO448_000016"
     CDS             13806..14156
                     /locus_tag="OO448_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCR6335353.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="OO448_000017"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="OO448_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCR6335354.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="OO448_000018"
     CDS             14475..14897
                     /locus_tag="OO448_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCR6335355.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="OO448_000019"
     CDS             14900..15532
                     /locus_tag="OO448_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCR6335356.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="OO448_000020"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="OO448_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCR6335357.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="OO448_000021"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="OO448_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCR6335358.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17318
                     /gene="pduO"
                     /locus_tag="OO448_000022"
     CDS             16311..17318
                     /gene="pduO"
                     /locus_tag="OO448_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCR6335359.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17315..18700
                     /gene="pduP"
                     /locus_tag="OO448_000023"
                     /pseudo
     CDS             17315..18700
                     /gene="pduP"
                     /locus_tag="OO448_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18711..19823
                     /locus_tag="OO448_000024"
     CDS             18711..19823
                     /locus_tag="OO448_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR6335360.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19820..21165
                     /locus_tag="OO448_000025"
                     /pseudo
     CDS             19820..21165
                     /locus_tag="OO448_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21168..21722
                     /gene="pduT"
                     /locus_tag="OO448_000026"
     CDS             21168..21722
                     /gene="pduT"
                     /locus_tag="OO448_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCR6335361.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21722..22072
                     /gene="pduU"
                     /locus_tag="OO448_000027"
     CDS             21722..22072
                     /gene="pduU"
                     /locus_tag="OO448_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCR6335362.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22077..22520
                     /locus_tag="OO448_000028"
     CDS             22077..22520
                     /locus_tag="OO448_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCR6335363.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22621..22950)
                     /locus_tag="OO448_000029"
     CDS             complement(22621..22950)
                     /locus_tag="OO448_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6335364.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23122..24180)
                     /locus_tag="OO448_000030"
     CDS             complement(23122..24180)
                     /locus_tag="OO448_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6335365.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24378..24851)
                     /gene="sbmC"
                     /locus_tag="OO448_000031"
     CDS             complement(24378..24851)
                     /gene="sbmC"
                     /locus_tag="OO448_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCR6335366.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24970..26136)
                     /gene="dacD"
                     /locus_tag="OO448_000032"
     CDS             complement(24970..26136)
                     /gene="dacD"
                     /locus_tag="OO448_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCR6335367.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26345..27772
                     /gene="sbcB"
                     /locus_tag="OO448_000033"
     CDS             26345..27772
                     /gene="sbcB"
                     /locus_tag="OO448_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6335368.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27815..28042)
                     /locus_tag="OO448_000034"
     CDS             complement(27815..28042)
                     /locus_tag="OO448_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6335369.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28056..29114)
                     /gene="yeeE"
                     /locus_tag="OO448_000035"
     CDS             complement(28056..29114)
                     /gene="yeeE"
                     /locus_tag="OO448_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6335370.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29293..30651)
                     /gene="plaP"
                     /locus_tag="OO448_000036"
     CDS             complement(29293..30651)
                     /gene="plaP"
                     /locus_tag="OO448_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6335371.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30641..30703)
                     /gene="yoeI"
                     /locus_tag="OO448_000037"
     CDS             complement(30641..30703)
                     /gene="yoeI"
                     /locus_tag="OO448_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6335372.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30918..31847)
                     /locus_tag="OO448_000038"
     CDS             complement(30918..31847)
                     /locus_tag="OO448_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6335373.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31893..32717)
                     /locus_tag="OO448_000039"
     CDS             complement(31893..32717)
                     /locus_tag="OO448_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6335374.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32800..33054)
                     /gene="yoeB"
                     /locus_tag="OO448_000040"
     CDS             complement(32800..33054)
                     /gene="yoeB"
                     /locus_tag="OO448_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6335375.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33051..33302)
                     /gene="yefM"
                     /locus_tag="OO448_000041"
     CDS             complement(33051..33302)
                     /gene="yefM"
                     /locus_tag="OO448_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6335376.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33585..33635
                     /gene="hisL"
                     /locus_tag="OO448_000042"
     CDS             33585..33635
                     /gene="hisL"
                     /locus_tag="OO448_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6335377.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33611..33734
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33781..34680
                     /gene="hisG"
                     /locus_tag="OO448_000043"
     CDS             33781..34680
                     /gene="hisG"
                     /locus_tag="OO448_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6335378.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34686..35990
                     /gene="hisD"
                     /locus_tag="OO448_000044"
     CDS             34686..35990
                     /gene="hisD"
                     /locus_tag="OO448_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6335379.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35987..37057
                     /gene="hisC"
                     /locus_tag="OO448_000045"
     CDS             35987..37057
                     /gene="hisC"
                     /locus_tag="OO448_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6335380.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37057..38124
                     /gene="hisB"
                     /locus_tag="OO448_000046"
     CDS             37057..38124
                     /gene="hisB"
                     /locus_tag="OO448_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6335381.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38124..38714
                     /gene="hisH"
                     /locus_tag="OO448_000047"
     CDS             38124..38714
                     /gene="hisH"
                     /locus_tag="OO448_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6335382.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38714..39451
                     /gene="hisA"
                     /locus_tag="OO448_000048"
     CDS             38714..39451
                     /gene="hisA"
                     /locus_tag="OO448_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6335383.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39433..40209
                     /gene="hisF"
                     /locus_tag="OO448_000049"
     CDS             39433..40209
                     /gene="hisF"
                     /locus_tag="OO448_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6335384.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40203..40814
                     /locus_tag="OO448_000050"
     CDS             40203..40814
                     /locus_tag="OO448_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6335385.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40911..41888)
                     /gene="wzzB"
                     /locus_tag="OO448_000051"
     CDS             complement(40911..41888)
                     /gene="wzzB"
                     /locus_tag="OO448_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6335386.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42034..43200)
                     /gene="ugd"
                     /locus_tag="OO448_000052"
     CDS             complement(42034..43200)
                     /gene="ugd"
                     /locus_tag="OO448_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6335387.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43450..44856)
                     /gene="gndA"
                     /locus_tag="OO448_000053"
     CDS             complement(43450..44856)
                     /gene="gndA"
                     /locus_tag="OO448_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6335388.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45020..46390)
                     /gene="cpsG"
                     /locus_tag="OO448_000054"
                     /gene_synonym="manB"
     CDS             complement(45020..46390)
                     /gene="cpsG"
                     /locus_tag="OO448_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6335389.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46495..47931)
                     /gene="cpsB"
                     /locus_tag="OO448_000055"
     CDS             complement(46495..47931)
                     /gene="cpsB"
                     /locus_tag="OO448_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6335390.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47934..49157)
                     /gene="wcaI"
                     /locus_tag="OO448_000056"
     CDS             complement(47934..49157)
                     /gene="wcaI"
                     /locus_tag="OO448_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6335391.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49154..49633)
                     /gene="gmm"
                     /locus_tag="OO448_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49154..49633)
                     /gene="gmm"
                     /locus_tag="OO448_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6335392.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49636..50601)
                     /gene="fcl"
                     /locus_tag="OO448_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49636..50601)
                     /gene="fcl"
                     /locus_tag="OO448_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6335393.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50604..51725)
                     /gene="gmd"
                     /locus_tag="OO448_000059"
     CDS             complement(50604..51725)
                     /gene="gmd"
                     /locus_tag="OO448_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6335394.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51751..52299)
                     /gene="wcaF"
                     /locus_tag="OO448_000060"
     CDS             complement(51751..52299)
                     /gene="wcaF"
                     /locus_tag="OO448_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6335395.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52315..53061)
                     /gene="wcaE"
                     /locus_tag="OO448_000061"
     CDS             complement(52315..53061)
                     /gene="wcaE"
                     /locus_tag="OO448_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6335396.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53072..54289)
                     /gene="wcaD"
                     /locus_tag="OO448_000062"
     CDS             complement(53072..54289)
                     /gene="wcaD"
                     /locus_tag="OO448_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6335397.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54264..55481)
                     /gene="wcaC"
                     /locus_tag="OO448_000063"
     CDS             complement(54264..55481)
                     /gene="wcaC"
                     /locus_tag="OO448_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6335398.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55478..55966)
                     /gene="wcaB"
                     /locus_tag="OO448_000064"
     CDS             complement(55478..55966)
                     /gene="wcaB"
                     /locus_tag="OO448_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6335399.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55969..56808)
                     /gene="wcaA"
                     /locus_tag="OO448_000065"
     CDS             complement(55969..56808)
                     /gene="wcaA"
                     /locus_tag="OO448_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR6335400.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56901..59063)
                     /gene="wzc"
                     /locus_tag="OO448_000066"
     CDS             complement(56901..59063)
                     /gene="wzc"
                     /locus_tag="OO448_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6335401.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59066..59509)
                     /gene="wzb"
                     /locus_tag="OO448_000067"
     CDS             complement(59066..59509)
                     /gene="wzb"
                     /locus_tag="OO448_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6335402.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59515..60654)
                     /gene="wza"
                     /locus_tag="OO448_000068"
     CDS             complement(59515..60654)
                     /gene="wza"
                     /locus_tag="OO448_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6335403.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60963..61112
                     /locus_tag="OO448_000069"
     CDS             60963..61112
                     /locus_tag="OO448_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335404.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61313..62896
                     /locus_tag="OO448_000070"
     CDS             61313..62896
                     /locus_tag="OO448_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6335405.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63158..65023)
                     /gene="asmA"
                     /locus_tag="OO448_000071"
     CDS             complement(63158..65023)
                     /gene="asmA"
                     /locus_tag="OO448_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6335406.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKMLVTR"
     gene            complement(65045..65626)
                     /gene="dcd"
                     /locus_tag="OO448_000072"
     CDS             complement(65045..65626)
                     /gene="dcd"
                     /locus_tag="OO448_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6335407.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65718..66359)
                     /gene="udk"
                     /locus_tag="OO448_000073"
     CDS             complement(65718..66359)
                     /gene="udk"
                     /locus_tag="OO448_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6335408.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66677..69994
                     /locus_tag="OO448_000074"
     CDS             66677..69994
                     /locus_tag="OO448_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6335409.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70104..70952)
                     /gene="alkA"
                     /locus_tag="OO448_000075"
     CDS             complement(70104..70952)
                     /gene="alkA"
                     /locus_tag="OO448_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6335410.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71086..72438
                     /gene="yegD"
                     /locus_tag="OO448_000076"
     CDS             71086..72438
                     /gene="yegD"
                     /locus_tag="OO448_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6335411.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72451..74391)
                     /gene="yegI"
                     /locus_tag="OO448_000077"
     CDS             complement(72451..74391)
                     /gene="yegI"
                     /locus_tag="OO448_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR6335412.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74388..75149)
                     /gene="pphC"
                     /locus_tag="OO448_000078"
     CDS             complement(74388..75149)
                     /gene="pphC"
                     /locus_tag="OO448_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR6335413.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75146..75805)
                     /locus_tag="OO448_000079"
     CDS             complement(75146..75805)
                     /locus_tag="OO448_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6335414.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76026..76085)
                     /gene="ibsA"
                     /locus_tag="OO448_000080"
     CDS             complement(76026..76085)
                     /gene="ibsA"
                     /locus_tag="OO448_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR6335415.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76358..76414)
                     /locus_tag="OO448_000081"
     CDS             complement(76358..76414)
                     /locus_tag="OO448_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6335416.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76685..76741)
                     /locus_tag="OO448_000082"
     CDS             complement(76685..76741)
                     /locus_tag="OO448_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6335417.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77020..78261
                     /gene="mdtA"
                     /locus_tag="OO448_000083"
     CDS             77020..78261
                     /gene="mdtA"
                     /locus_tag="OO448_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR6335418.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78261..81383
                     /gene="mdtB"
                     /locus_tag="OO448_000084"
     CDS             78261..81383
                     /gene="mdtB"
                     /locus_tag="OO448_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6335419.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81384..84461
                     /gene="mdtC"
                     /locus_tag="OO448_000085"
     CDS             81384..84461
                     /gene="mdtC"
                     /locus_tag="OO448_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6335420.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84462..85877
                     /locus_tag="OO448_000086"
     CDS             84462..85877
                     /locus_tag="OO448_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6335421.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85874..87277
                     /gene="baeS"
                     /locus_tag="OO448_000087"
     CDS             85874..87277
                     /gene="baeS"
                     /locus_tag="OO448_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6335422.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87274..87996
                     /gene="baeR"
                     /locus_tag="OO448_000088"
     CDS             87274..87996
                     /gene="baeR"
                     /locus_tag="OO448_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6335423.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21560 a        23316 c        22148 g        21054 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact accctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgag agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaagcgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa gacaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccaat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcag ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt
    16681 tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca acggaaacca
    16741 ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg
    16801 cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt
    16861 atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg
    16921 atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat attcccgttg
    16981 tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc
    17041 tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg
    17101 caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg ctcgaaagcc
    17161 atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat
    17221 tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa
    17281 gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact
    17341 gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc aaagaaacgc
    17401 tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt tacgctttca
    17461 acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc
    17521 ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga
    17581 taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag atttaaccac
    17641 cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt ttggggtgat
    17701 tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat
    17761 gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa acgtttcgtt
    17821 aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacttgat
    17881 cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaaat
    17941 cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga aaagtggcaa
    18001 gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa cggcggatat
    18061 tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat
    18121 tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc agcagatgca
    18181 aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg caatttgcct
    18241 gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc
    18301 ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg tcgacgccga
    18361 tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga aggtcaagga
    18421 ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc acacggcaat
    18481 tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc aaacctcaat
    18541 atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac
    18601 ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt ttgcccgctc
    18661 acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa atgaatactt
    18721 tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt agccgtttct
    18781 ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca cttattgaca
    18841 cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg
    18901 ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta cgcccggatg
    18961 tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt gtctggttcg
    19021 gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtatcg
    19081 gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt aaatatcccc
    19141 ttttcgataa tgcactttat ccggatattg cgatccttga tccatctttg gtcgtcagcg
    19201 tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt
    19261 atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg
    19321 tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc cgtggcaaaa
    19381 tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc ggtttaaacc
    19441 atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg gcgaatgcgc
    19501 tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc aaacgctatg
    19561 cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga
    19621 atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg actcttgcca
    19681 atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcgg
    19741 cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc gcgattgcag
    19801 aactgctcaa ggggttgtta tgaacgtcgg attaatctcc tgcgacgacc agaccatccg
    19861 cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat
    19921 taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg aacccatgtt
    19981 gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg gtgttcacta
    20041 tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg
    20101 ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga
    20161 cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgttcc
    20221 acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa
    20281 tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ccgttaatgg
    20341 tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct
    20401 ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg ggccgatgat
    20461 gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg gcttgctggt
    20521 tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc
    20581 cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt
    20641 aatcggtcac gaactggcac cccatttatt agtacgtgcg cggttaacta ccaacagctc
    20701 gccacgccgc aactgttact gacagcgctc acctgttcag agtgcaacgt ctgcgccagt
    20761 gtcgcctgtc cggtaggaat ttcaccaatg cgcataaacc gcctgcttaa gcaggaatta
    20821 cgcgcacaaa atctccgcta cgaaggtgcc ttaaatcctg cagatcccat ggcaaattat
    20881 cgactgattc ccgtaaaacg gctggtaacc agacttggct taaccccgtg gtatcaggat
    20941 gcgccattgt ctgaacaggt gcctcagccc gaaaaagtga cgttattgtt acgccagcac
    21001 atcggtgcca gcgcaattgc gtgtgtacaa aaaggcgatc gcgtcgttca tgggcaatgt
    21061 gtaggtcaaa ttccacacgg cacattaggt gcccccattc atgccagtat tgatggcatg
    21121 gtcagcgatg tgacagaaaa cgcgattacg cttgtgagag gttaaccatg tcgaaagcgg
    21181 taggtatttt agaacttagc agtatcgcga aagggatgga aaccggcgac gccatgctga
    21241 aaagcgccaa cgttgagtta ctggtcagca aaacgctatg ccccggaaaa tttctgctga
    21301 tgctcggtgg cgatgtgggc gcggtgcaac aggccatcaa cagcggagcg tcccttgctg
    21361 gtgaaatgct ggtcgacagt cttgtcctgc cgaatattca tcccagcctc ctttctgcaa
    21421 tcagcggact taaccatgtt gaacagcacc gggctgtcgg cgtagtcgaa acctggagtg
    21481 tcgccgcctg cattgacgct gccgattgtg ctgtcaaagc tgcaaatgtc attcttgttc
    21541 gcgtacatat ggcttttggc attggcggta aatgctacat ggtggttgct ggcgatattt
    21601 cagatgttaa taacgcggtc tctgccgcca gcgtgcgcgc gggggaaaaa ggtttgttgg
    21661 tctatcggtc ggtgattgcc cgtccacatg aagccatgtg gcgtcagatg gtggagggat
    21721 aatgacaact cagcacacaa ctgaacggat gattcaggaa tatgtcccag gtaagcaggt
    21781 cacgctggca catgtcatcg ccaatccagg gaaggatctg tttaagaaat tgggtttacc
    21841 agacaccatt tccgctattg gtattctgac aattaccccc agcgaagcat ctatcatcgc
    21901 atgcgatatc gccacaaaat caggcgctgt agagatcggt tttcttgatc gttttaccgg
    21961 tgccgtagtg ctgactggtg atgtttctgc gatcgaatat gcacttagcc aggttacccg
    22021 tacgctgggt gaaatgatgc gctttaccgc ctgccccatt acgcggacct gacgttatgc
    22081 aacgtatgat gctcatcggc cccagccagt gcggaaaaac gtcactcacg caatgtctgc
    22141 gtggcgaaaa tattcattat cagaaaacgc aggcaatcgt ctggtccccg accaccattg
    22201 atacaccagg cgaatacctc gaaaaccgca gcctctacag tgcactgttg gtcagtgcct
    22261 gcgaagcgga cattatcgcg ctggtgctta atgcggacgc accgtggtca cctttttcgc
    22321 caggctttac ctcgtcaatg aatcgcccgg tcattggagt agtaaccaaa gcagatttag
    22381 ccaacatgga acaaatatct ttggtgaaat gttggttacg ggaagctgga gcgcacaacg
    22441 tgttagtaac cagtgcggtt aataaccatg gagttgcaga acttttagcc ttgctgcata
    22501 cagaagaagg ctgtcgctaa tttcgacagg ttatcagatg ggaatgatga cggtgataca
    22561 cagaaaagaa tcaatgagca atgagggttg ccgggcaacc ctcattgaat aaaacaggaa
    22621 ttacttcgct tcgccgtttt tcatttcgcc catagctttc agctttttgg agatatcgcg
    22681 gcgttctttg gagagctcgg catttttgat gatgtaatcg tcaacgcgat cttcatagtc
    22741 acctttcatg ctggcgatga tgccctggat tgcttcaacg ctcatcccag gcttgatgta
    22801 atcgctcagg ttgtccagca gcaggacgcg cttctggttg tcacggatct ttttctcaac
    22861 gtcctgaatt tcacgttgca gtttgttctt acgacggaac agacgaacaa attccagtac
    22921 gtcctggaat gaaggcttgg tagtttccat ttttataccc ctgataatgt gagagtcgga
    22981 ttcgtttaag caaccgctat tcgttagggc caacattact catagctgcc gcagatgaca
    23041 atgcttttat cccttttcac tatcataccc tttatccttg ctgaatcgaa gcagcagcaa
    23101 gatgattctg aagttcagga attatttgcg caaggcccgg caaatcagat tcgatagcaa
    23161 ctcaagctga tgcgccgttt ccatgttcag ccacacataa ccatagattg gtgtttccac
    23221 taccttcagg tcatggtggt tattgagcaa ctgacgcagc tcttccacag catcgttcaa
    23281 tttttccgta ttggcaaaaa ccggctgcgg attaccttcg tacagcgcat gaacaaagct
    23341 cagcagtatt tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac
    23401 gaaatggctg gggcgcgtgg cccagtacgc attgatttgc aactccagca tacaaaccag
    23461 attgcggtta atggtctgga taccttcata tatcgatttt ggaatacggg tttctttgct
    23521 ggcgggcgca atcagcccac gcattttcac ggcatcggtc aggagttttt gtagatggct
    23581 ttccagacgt gggcgttcga gtaagttcgg tgagaatgca gattgataga cccgattata
    23641 ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt gtggccagat
    23701 accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc gccataacgc
    23761 cgtatcaatt tcacctgtcg gggaaccaac aacaattgcc agcgtcaccc caatcaataa
    23821 tccttgatac ggtttcttgc ccagcgccag ccaaccgcaa agaaacatcg ccgccgcgca
    23881 ccagactaac atcagcggta acgaaattaa ctccagttgc agagcgataa gacctaaaat
    23941 cgaacccaac accgtaccgc caatacgttc gaaggcacga gggacaacgt taccccagaa
    24001 cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt ccgggatagt
    24061 aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag taccatgcac
    24121 tatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat cggcacgcac
    24181 gcagaaactc cgtaatgaag attaaaagcg ctcataactc atctgtcggc cagaaggcgc
    24241 gcctctgaat tatccgacgg acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa
    24301 aattgaaagg cgcatcttac tcttttcgct ttcaaaaaaa gagtggtcat cgcgttaaca
    24361 caccgccctg agatgaatta gtgatgtttt ggctgcaccg caacatacat ttcgatatcc
    24421 cagtacccat cttccgcgcc attgttcaaa taaacctcga agcagggctt tggtaacatt
    24481 tcataagcac tgtcctgcaa gaggctatta aagaactgat accagggttt agcaaaatca
    24541 tcaccgacta cacgagccac cgccaccgca tactgaccac ctgaaatttc tgtcagaatg
    24601 acgccctcac tgttttcggg aagcgtaaag ttattcggta ccgtcacaac ggtgtcgcag
    24661 cgtaattttt cggcgggtgt ttcatctgga ttgtcgtaat agacagcaac ccactccttc
    24721 ggcacaatat ttttgttatc tacccacatc atcaactgct caaagccttt ctttaccgtc
    24781 tgttcccacg ggccaacgag atggaaacct gcaacggtac gtttctcttc ctgcgtaatc
    24841 tcgtagttca tgacgcctcc attaacactg tttttatata cagcatagtt gcaatattaa
    24901 aaccacaagg aatgagtgtt gattatgcga gcagactcgc actcctgcca gtctgctgca
    24961 aaagaaaggt caggccttat ggtggaaata atcactcagg cgggaaaaca tgccgccttc
    25021 cccgacagat tccagggtaa ccagcggcca gtgcgccacc tgtttatcac ggtcgtaaag
    25081 ttcaatttcc cctacccgct gatgggcgct aattggcgcg gtgagctctt taccatcaag
    25141 ggtatatttg gctttgatat gtggaatttc ggctttcggt agcaccatcc agaactcttg
    25201 ttccgttcct aggtcgatat tttctttatc gccataccag atgcgttccg ttccgacctt
    25261 tttcccacgg tgcaaaattt gcaccgtagt aaagttttgt tgcccccaac gcagtaattt
    25321 tcttgcctct tcctcacgac cttttgcact gtcagccccc attaccactg caatgagacg
    25381 acgctgccca tctacagccg aagcaatgag attaaacccg gcaccagaag tatgacccgt
    25441 tttcaggccg tcaacattca tggttttatc ccacaacaac ccgttacggt tttgctgggt
    25501 gataccgttc caggtgagac ttttctcact gtacatatga taaaactcgg gctcgccgtg
    25561 gatgatagcg cgagaaaaca cagctaaatc ataagccgag ctatgctggc caggtgcatc
    25621 cagaccatgc actgtttcaa aatgcgtatc cttgagatgc agcttctcgg catagttgtt
    25681 catcatttca acaaactgcc gttgcccacc ggcaatatag tcagccagag caacacaagc
    25741 gtcatttccg gaatccacaa ttaaaccacg gcttaaatca cgtaccgata cgcgatcgcc
    25801 ctctttcaaa aacatcagtg aagaaccgac aaacaccgga ttatctttcg cccacgcatc
    25861 gcgccccacg gtgacaatat cgtctggcgt aatgcgatga ctatcgatag cgcgatccac
    25921 gacataaccc gtcatcagct ttgtcaggct ggcgggattg cgctgttgat gctcattacc
    25981 cgcggtgagg atctgaccgg tggtgtaatc catcaatacc caggacccgg catgaatctc
    26041 tggaggctga ggtgaaaaag gaatgttttc cgccgcaaaa ccagacgata agttaaaaac
    26101 gaacaaagaa gcagcaataa taagacggcg tttcaacagc aaaccctcag gagtttcaaa
    26161 tagctgttct ttttacggaa atacttatga actggctgga ataaagtgca agaaaatgtg
    26221 actaccctct catttttatc tgacatgatc tgttgccact cgctgccaaa ttgtggcgct
    26281 aaagctgatt agcacggtga tatttgatac tctggcagac agcagaaata acggatttaa
    26341 cctaatgatg aatgacggta agcaacaatc tacctttttg tttcacgatt acgaaacctt
    26401 tggcacgcac cccgcgttag atcgccctgc acagttcgca gccattcgca ccgataacga
    26461 attcaatgtc atcggcgaac ccgaagtctt ttactgcaag cccgctgatg actatttacc
    26521 ccagccagga gccgtattaa ttaccggtat taccccgcag gaagcacggg cgaaaggaga
    26581 aaacgaagcc gcgtttgccg cccgtattca ctcgcttttt accgtaccga agacctgtat
    26641 tctgggctac aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt tttatcgtaa
    26701 tttctacgat ccttacgcct ggagctggca gcatgataac tcgcgctggg atttgctgga
    26761 tgttatgcgt gcctgttatg ccctgcgccc ggaaggaata aactggcctg aaaatgatga
    26821 cggtctaccg agctttcgcc ttgagcattt aaccaaagcg aatggaattg aacatagcaa
    26881 cgcccacgat gcgatggctg atgtgtacgc cactattgcg atggcgaaac tggtaaaaac
    26941 gcgtcagcca cgtctgtttg attatctctt tacccatcgt aataaacaca aactgatggc
    27001 gttgattgat gttccgcaga tgaaacccct ggttcacgtt tccggaatgt ttggggcatg
    27061 gcgcggcaat accagctggg tggcaccgct ggcgtggcat ccagaaaatc gcaatgccgt
    27121 aattatggtg gatttggcag gagacatttc gccattactg gaactggata gcgacacatt
    27181 gcgcgagcgt ttatatactg caaaagccga tcttggcgat aacgccgccg ttccggttaa
    27241 gctggtgcat atcaataaat gtccggtgct ggcccaggcg aatacgctac gcccggaaga
    27301 tgccgaccga ctgggaatta atcgtcagca ttgcctcgat aacctgaaaa ttctgcgtga
    27361 aaatccgcaa gtgcgcgaaa aagtggtggc gatattcgcg gaagccgaac cgtttacgcc
    27421 ttcagataac gtggatgcac agctttataa cggctttttc agtgacgcag atcgtgcagc
    27481 aatgaaaatt gtgctggaaa ccgagccgcg taatttaccg gcactggata tcacttttgt
    27541 cgataaacgg attgaaaagc tgttgttcaa ttatcgggca cgcaacttcc cggggacgct
    27601 ggattatgcc gagcagcaac gctggctgga gcaccgccgc caggtcttca cgcccgagtt
    27661 tttacaaggt tatgcagatg aattgcagat gctggcacaa caatatgccg ataacaaaga
    27721 gaaagtggcg ctgttaaaag cgctttggca gtacgcggaa gagatcgtct aatttgaagc
    27781 ctccgccgct ggtacggcgg aggtcaatgt tgagtcaggc tttttgaacg gtgatactcc
    27841 acgctgcatc gccaatctgc tgataatcgg tgatggcatg cccttcttct gccgcccact
    27901 gtgggattgc ttcagttgcc tgggtgcaat caaactcaat taccaactca tcgccgctgg
    27961 ccatttctgc cagtgctgct tttgcttcta tgagtggaaa cggacagacc tgcgtcacca
    28021 catccagctt tttaatcgcc atctttacac cttacttaat ttgccgcagc agttgccagt
    28081 cgcgccttac gctgcggtcg aacatacaca agccaggacg cagtccagac tccgagaatc
    28141 ataaatacaa ggccaatcca gccctgccag gtcatcattg cagtcataac cagtccatta
    28201 ccgataaagc aaccacctgc aatactggca ccgaagccca tcagtacacc acctaacccg
    28261 ctacgtaatg ttgtttgtgc atcagctgcg cgaacgcgga actcacggct cgctttggct
    28321 gcaataaatg accccacgaa gatccctaac accaggaaaa cgccccagtt aatgtatttc
    28381 acgtcacccg cgaccagaaa ttgcagaata ttggccgttg gggaagtgat tcctaaccca
    28441 aacatgcgtc cggttgcttc actcaggggc caggctaaaa gcgcaatcaa accgatgagt
    28501 acagcagtga caaagggatg ccagcgtttt tcaaacagaa tatgagcgat cccagttcgg
    28561 cgcggcggta aggtcgcgac tttgagtttt ggcttcttca actctttcat caccacccag
    28621 agcgttatca ccagcaaaac ggcaaccaac ggccaaacag acaaattaaa agtctcagca
    28681 atagagttat gttcagtact gtagtgctgc aaggtttgat ttaaaccact ggcatgtgga
    28741 gaacgcatca ccgcactcat caccatataa gtgaaaagcg cgatccaact gccgatcaat
    28801 ccctcacccg cgcgatacca ggtcccggtg gcacatccac ccgccagaac aattcccagc
    28861 ccgaagatat acccacctat aacagtacca agccacggga acgcctcggc ttcgtaagtc
    28921 agtaggcccg cctgaattaa cgcaaaaacc cccacgcttt gtaccgaaat tgcaatcagc
    28981 aaggcgtaaa acatgcgatt atttttcgcg atatacatat cgcgaaaacc acctgtcagg
    29041 caaaaacgcc cacgctgcat gacaaatccc agcagagcac cacaaattag cccgcttaat
    29101 atcattgaaa acataaatta attaaccaga tgaatgttaa tgaggaaatt attcatgact
    29161 ggtggaatgc agaccaataa ccaaattctc taaattagaa caaatggtta ttaatgagga
    29221 tttcgataaa aaaaaataaa aaaaccggag ccaaatattc attcgactcc ggttgattat
    29281 tagaaaagat gattatgcaa cgtcttcgta ctgcggaact gggttacgga agctcttggt
    29341 gacgcaagcc aggtaaatca gaccgatagc agcccaaatc agacccagaa ccattgagct
    29401 ttcttccagg ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag
    29461 atactggaag tgatctttca gcgtcttatt acgcttctca cggatccaga actgcgagat
    29521 gaccgacagg ttaacgaagg tgaacgccac caacgcacca aagttaatca gcgctgtagc
    29581 cattaccagg tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga tgatgttcat
    29641 cgccggagta cgccatttcg ggtgtacata accgaagaag cttttcggga atacgccgtc
    29701 acgacccatt acgtacatca gacgcgctac gcctgcatgc gccgccatac cggacgccag
    29761 tacggtaatg gtggagaaga tcagcgcgcc aacctggaac gctttacccg caacgtacag
    29821 catgatttca ggctgtgacg catccggatc tttaaagcga gagatatccg ggaagtacag
    29881 ctgcaggaag taagttgcaa agatgaagat catgccgcca atcagcgcgg tcaggaaaat
    29941 cgcacgcggg atcacgcgct ctgcatcttt ggtttcttcc gacaggttgc tgatgccgtc
    30001 aaagccggta aaggaaaagc acaggattgt cgccccggta atcatcggaa taacatgcgc
    30061 atcgccagac cagaacggac gagtgctcgc cagcgtacca gcgccttcac cttcaaatac
    30121 gccataaaca accatgccca gaatcaccgc gatcagcact acctgcaaca cgacgattac
    30181 agtgttgaag ttcgctacgg atttcagact acgcaggtta aaggcggtca taaaggccac
    30241 cagcgccacc acaaacatcc acgatgggat ggaaggcacc agagcttcaa aatagatttt
    30301 cgccagcaga atgttgatca tcggcgcgaa cagatagtcg agcagagaag accaacccac
    30361 cataaagccg acagtcgggc taatggattt ctgggcgtaa gtatatgcag agccagcaga
    30421 aggatagcgg cgaaccagct tcccgtagct cagagccgta aacaggatcg caatcaatgc
    30481 gaacgcatag gctgtcggca catgaccatc cgtgaggcct gaaacgatac caaaagtatc
    30541 aaacagcgtc atcggctgca tataggcaag acccatcatt acaaccggaa ctaacgtaag
    30601 cgttttacgc aattccacgc gagaggtgtt tggagtaacg ttatgcgaca tggtcattct
    30661 cctttacggt gataaccgtc gcgtaagcaa aaaattgccc catttttttg gattcctcag
    30721 cgacaacaac tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta
    30781 tgaatgattg gtttgaagca aaaaataacc gacgctgatg aaacgtcggt ttttagtcat
    30841 tttttgacag cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaagctt
    30901 gtcataataa tttttcttta aatggctgat tttccgtcat cagattttgc ataaacaccg
    30961 gcgatcgcac tatttgctaa aatttcatcc cgccaccagc cgctggcaag ccctgcggtc
    31021 tgttcgttcc agccaatcca gacgggttca aagagagttt gggcgaccac tttcttctcg
    31081 attaacgcgc cactatcgag aaaacgttgc accagataac ggggtaaata gccgcagccg
    31141 aggccgctaa tttgcagctc cagcttggtt ttaaaatcga aaacggtaat ggcttcttgt
    31201 tcgtcgagaa gttgtgaggc cgtagaagcg cctgcctgcg cagtgtcccc caccacaatc
    31261 gcccgatatc gcttaatgat acgacggttt aacggctctt cttcctgggc taacggatga
    31321 tgaggcgcga ccgcaaagac ttgttccaga tcgcccaacc gcgagaaacc aaactcactg
    31381 gaagatggtg gctcatgcat cgcgccaaca ataatatccg ctcgcccctg agtcagggca
    31441 tcccaggaac caccgagtac gccattgata aacttcaggc gcgtcacgct atgatgttga
    31501 taaaaagctt caatcagtgg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc
    31561 accagctcgt tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg
    31621 gtatgcagaa cttcccgccc cttctctaat aacatttttc cggtacgggt gaatttagcg
    31681 cggtgaccgc tacgatcaag taattgaata ttaaggtcgc tttccagctt gtgaacggtg
    31741 taactcagag cggacggcgt cttgtagagt ttagccgacg ccgccgcaaa actgccttct
    31801 ttttctaagg catcgaggat catcaacaca tccagcagtt gtttcataca cgcccccttg
    31861 cagtgcatgc ggcgtcctgc tggcgtgatc gtttactcca gcggcattac cagcggatcg
    31921 ggatactggt attcaaatcc cagttcatta caaatccgac tgccatcaat aatcttacct
    31981 ttgccgctgt ccagactatt tctgaactgc ggaggttcca gccccagtaa acgggccatc
    32041 tgcggataga aaacattacg cgcagggtga gctggcgcac atatattata gatgtgtccg
    32101 cctttaggtg cctgtaacaa cagagtgata gcgccaataa catcttctaa atggactaaa
    32161 ttaacaccat gttcaccatc aggcgcggtt tttccggcaa agaagcgtcc gggatgacgt
    32221 cccggtccga ccagccccgc aagacgcaga atatcgaccg aagtaccggg taaattgtgc
    32281 agccagtctt cgagttcttc taatactcgt ccactgttgg tcaccggatt acgcggggtg
    32341 gtttctttca ccgtgccttg cgcgtcgcca tagacagacg ttgagctggt aaaaataatg
    32401 cgaggaatac gatgggccag cgcgctatcc actaactctt gtaccgcttg taaatagaac
    32461 tcatcgccgg ggccgctacg acgtgccgga agcgtaatga ccagcgcatc ggcatccatc
    32521 agggcatcca gatcgtcaga atcgcaaact aactcaggct ccatgcgaag cagatagcta
    32581 tcaatgccac tcattcgggc cgcttcgaca ccatcttgtg tggttttact cccggtgact
    32641 tgccagcctc gcgctgaaag tgacatcgcc agcggcatgc ctaaccaccc taacccgaca
    32701 attgcgacct ttttcatccg ttatctcctg atacacctta gatctataag gctacgctag
    32761 cgtatcaaaa ctgacaattc attctatgaa tgaatctgtt caataatgat aacgacatgc
    32821 tgcaatgagc agtgaatcgt cggtaaccgc gtataccaga cggtgctcct ctgtaatgcg
    32881 tcgggaccag aaacctgaca aattatgttt caggggttct ggcttcccct taccttcaaa
    32941 tggcgttctg cgggtatctt tgataagttc attgatcttt ttaacaattc gcttatctgt
    33001 ttcctgccag tacagataat catcccatga ttcctcagac cagattagtt tcactcaata
    33061 atgtcctttt ctgttccttt gcctgatttc aggctatcga ttgagtccat caatctccgg
    33121 gcgttagcgg gggagcgcag tagataagcc gtctcttcca gcgagttgta ttcttcgagt
    33181 gacatcagaa cacaagcctc tccattctga cgagtaataa ggatcggggc atgatcttca
    33241 acggctttca tcattgttgc cgacaaattc tgacgcgctt cgctgtagct aattgtacgc
    33301 atgtcaatct cctcttttgt acagtttatt gtacaatgat gggcgttaat taactattta
    33361 ttaattagtt tgtagatcaa ggtattgtca gtgagacgaa aatccaggct tcgctatttt
    33421 tggtgccgtc agcttagagg acagtcctct tagccccctc ctttccccgc tcattcatta
    33481 aacaaatcca ttgccataaa atatataaaa aagcccttgc tttctaacgt gaaagtggtt
    33541 taggttaaaa gacatcagtt gaataaacat tcacagagac ttttatgaca cgcgttcaat
    33601 ttaaacacca ccatcatcac catcatcctg actagtcttt caggcgatgt gtgctggaag
    33661 acattcagat cttccagtgg tgcatgaacg catgagaaag cccccggaag atcaccttcc
    33721 gggggctttt ttattgcgcg gttgataacg gttcagacag gtttaaagag gaataacaaa
    33781 atgacagaca acactcgttt acgcatagct atgcagaaat ccggccgttt aagtgatgac
    33841 tcacgcgaat tactggcgcg ctgtggcatt aaaattaatc ttcacaccca gcgcctgatc
    33901 gcgatggcag aaaacatgcc gattgatatt ctgcgcgtgc gtgacgacga cattcccggt
    33961 ctggtaatgg atggcgtggt agaccttggg attatcggcg aaaacgtgct ggaagaagag
    34021 ctgcttaacc gccgcgccca gggtgaagat ccgcgctact ttaccttgcg tcgtctggat
    34081 ttcggcggct gccgcctttc gctggcaacg ccggttgatg aagcctggga cggcccgctc
    34141 tccttaaacg gtaaacgtat cgccacctct tatcctcacc tgctcaagcg ttatctcgac
    34201 cagaaaggca tctcttttaa atcctgctta ctgaacggtt ctgttgaagt cgccccgcgt
    34261 gccggactgg cggatgcgat ttgcgatctg gtttccaccg gtgccacgct ggaagcaaac
    34321 ggcctgcgcg aagtcgaagt tatctaccgc tcgaaagcct gccttatcca gcgcgatggt
    34381 gaaatggaag aatccaaaca gcagctgatc gacaaactgc tgacccgtat tcagggtgtg
    34441 atccaggcgc gcgaatcaaa atacatcatg atgcacgcac cgaccgaacg tctggatgaa
    34501 gtcatcgccc tgctgccagg tgccgaacgc ccaaccattc tgccgctggc gggtgaccaa
    34561 caacgcgtag cgatgcacat ggtcagtagc gaaaccctgt tctgggaaac gatggaaaaa
    34621 ctgaaagcgc tgggtgccag ttcaattctg gtcctgccga ttgagaagat gatggagtga
    34681 tcgccatgag ctttaacaca atcattgact ggaatagctg tactgcggag caacaacgcc
    34741 agctgttaat gcgcccggcg atctccgctt ctgaaagcat tacccgcact gttaacgata
    34801 ttctcgataa cgtgaaagca cgcggtgatg acgccctgcg ggaatacagc gcgaagtttg
    34861 ataaaaccac ggttaacgcg ctgaaggtgt ctgctgagga gattgccgcc gctagcgaac
    34921 gcctgagcga cgagctaaaa caggcgatgg cggtggcagt aaaaaatatt gaaaccttcc
    34981 acactgcgca aaaactgccg ccggtagatg tagaaacgca gccaggcgtg cgttgccagc
    35041 aggtcacgcg cccggtcgct tcagttgggt tgtatattcc tggcggctcc gccccgctct
    35101 tctcaacggt attaatgctg gcgactccgg cgcgtattgc gggctgtaaa aaagtggtgc
    35161 tgtgctcacc gccgccgatt gccgatgaga tcctttatgc ggcgcagctg tgcggtgtga
    35221 aggacgtgtt taacgtcggc ggcgcacagg ccattgccgc gctggcgttt ggtacggaat
    35281 ctgtgccgaa agtggacaaa atcttcgggc cgggtaacgc ctttgtcacc gaagcgaaac
    35341 gtcaggtgag ccagcgtctg gacggtgcgg ctatcgatat gcccgcaggc ccgtcggaag
    35401 tgttggtcat tgccgacagc ggcgcaacgc cggatttcgt ggcttctgat ttgctttctc
    35461 aggctgaaca cggcccggac tcacaggtga ttttactgac gcctgacgct gatatggcgc
    35521 atcaagttgc cgaagccgtc gaacgccagt tagcagaact gccgcgtgcc gaaaccgccc
    35581 gccaggcact gaacgccagc cgcctgatcg tgactaaaga tttagcgcag tgcgtagaga
    35641 tctccaacca gtacggcccg gagcacctga tcatccagac ccgcaacgcc cgcgatctgg
    35701 tcgatggcat caccagcgcc ggttcggtat ttcttggtga ctggtcaccg gaatcggcag
    35761 gtgattacgc ctccggcacc aaccacgttc tgccgactta cggttacacc gccacctgtt
    35821 ccagcctcgg gctggcagat ttccagaagc gtatgaccgt gcaggaactg tcgaaagaag
    35881 gtttctccgc gctggcttcg accattgaaa cattggccgc cgccgagcgc ctgaccgccc
    35941 ataaaaatgc cgttactttg cgtgttaacg cccttaagga gcaagcatga gcaccgtgac
    36001 tattaccgat ttagcgcgtg aaaacgtccg caacctgacg ccgtatcagt cggcgcgtcg
    36061 tctgggcggt aatggcgacg tctggctgaa cgccaacgaa taccccactg ccgtggagtt
    36121 tcagcttact cagcaaacgc tcaaccgcta cccggaatgt cagccgaaag cggtgattga
    36181 aaattacgcg caatatgcag gcgtaaaacc ggaacaggtg ctggtcagtc gtggcgcgga
    36241 cgaaggtatt gaactgctga ttcgcgcttt ttgcgaaccg ggtaaagacg ccatcctcta
    36301 ctgcccgcca acgtacggca tgtacagcgt cagcgccgaa acgattggcg tcgagtgccg
    36361 cacagtgccg acgcaggaca actggcaact ggacttacag ggcatttccg acaagctgga
    36421 cggcgtaaaa gtggtctatg tttgcagccc caacaacccg acagggcaac tgatcaatcc
    36481 gcaggatttt cgcactctgc tggagctaac ccgcggaaaa gccattgtgg ttgccgatga
    36541 agcctatatc gagttttgcc cgcaggcatc gctggctggc tggctgacgg aatatccgca
    36601 cctggctatt ttgcgcacac tgtcgaaagc ttttgctctg gcgggccttc gttgcggatt
    36661 tacgctggca aacgaagaag tcatcaacct gctgatgaaa gtgatcgccc cctacccgct
    36721 ctcgacgccg gttgccgaca ttgcggccca ggcgttaagc ccgcagggaa tcgtcgctat
    36781 gcgcgaacga gtgacgcaaa ttattgcaga acgcgaatac ctgattgccg cactgaaaga
    36841 gatcccctgc gtagagcagg ttttcgactc cgcaaccaac tacattctgg cgcgctttaa
    36901 agcctccagc gcagtgttta aatctttgtg ggatcagggc attatcttac gtgatcagaa
    36961 taaacaaccc tctttaagcg gctgcctgcg aattaccgtc ggaacccgtg aagaaagcca
    37021 gcgcgtcatt gacgccttac gtgcggagca agtttgatga gtcagaagta tctttttatc
    37081 gatcgcgatg gaaccctgat tagcgaaccg ccgagtgatt ttcaggtgga ccgttttgac
    37141 aaactcgcct ttgaaccagg cgtgatcccg gaactgctga agctgcaaaa agcgggctac
    37201 aaactggtaa tgatcaccaa tcaggatggt ctgggaacac aaagtttccc gcaggcggat
    37261 ttcgatggcc cgcacaacct gatgatgcag atcttcacct cgcaaggcgt acagtttgat
    37321 gaagtgctga tttgtccgca cctgcccgcc gatgagtgcg actgccgtaa gccgaaagta
    37381 aaactggtgg aacgttatct ggctgagcaa gcgatggatc gcgctaacag ttatgtgatt
    37441 ggcgatcgcg cgaccgacat tcaactggcg gaaaacatgg gcattaatgg tttacgctac
    37501 gaccgcgaaa tcctgaactg gccaatgatt ggcgagcagc tcactaaacg agaccgttac
    37561 gctcacgtag tgcgcaacac caaagagaca cagattgacg ttcaggtgtg gctggatcgc
    37621 gaaggtggca gcaagattaa caccggtgtt ggcttctttg atcacatgct ggatcagatc
    37681 gccacccacg gtggtttccg tatggaaatc aacgtcaaag gcgacctcta tatcgacgat
    37741 caccacaccg tcgaagatac cggcctggcg ctgggcgaag cgttaaaaat ggctcttggc
    37801 gacaaacgcg gtatttgccg ctttggtttt gtgctaccga tggacgaatg ccttgcccgc
    37861 tgcgcgctgg atatctctgg tcgcccgcac ctggaataca aagccgagtt tacctaccag
    37921 cgcgtgggcg atctcagcac cgagatgatc gagcacttct tccgttcgct ctcatacacc
    37981 atgggcgtga cgctacacct gaaaaccaaa ggtaaaaacg atcaccaccg tgtagagagc
    38041 ctgttcaaag cctttggtcg caccctgcgc caagccatcc gcgtggaagg cgacaccctg
    38101 ccctcgtcga aaggagtgct gtaatgaacg tggtgatcct tgataccggc tgcgccaatc
    38161 tgaactcggt gaagtcagcc attgcgcgcc acggttatga accaaaagtc agccgtgacc
    38221 cggacgtggt gttgctggcc gataaactat ttttacccgg cgttggcact gcgcaagcag
    38281 cgatggatca ggtgcgtgag cgcgagctgt ttgatctcat caaagcctgt acccaaccgg
    38341 tactgggcat ctgcttaggg atgcaactgc tgggtcggcg cagcgaagag agcaacggcg
    38401 tcgacttgct gggcatcatt gacgaagacg tgccgaaaat gaccgacttt ggtctgccgc
    38461 tgccgcatat gggctggaat cgcgtttacc cgcaggcagg caaccgcctg tttcagggga
    38521 ttgaagacgg cgcgtacttt tactttgttc acagttacgc aatgccggtc aatccgtgga
    38581 ccattgccca atgtaattac ggcgaaccgt tcaccgcggc ggtacaaaaa gataacttct
    38641 acggcgtgca gttccacccg gagcgttctg gtgccgctgg cgctaagttg ctgaaaaact
    38701 tcctggagat gtgatgatta ttccggcatt agatttaatc gacggcaccg tggtgcgtct
    38761 ccatcagggc gattacggca aacaacgcga ttacggtaac gacccgctgc cgcgcttaca
    38821 ggattacgcc gcgcagggtg ccgaagtgct gcatctggtg gatctgaccg gggcaaaaga
    38881 tccggcaaaa cgtcaaatcc cgctgattaa aaccctggtc gcgggcgtta acgttccggt
    38941 gcaggttggc ggcggtgtgc gtagcgaaga agacgtggcg gcgttactgg aagcgggcgt
    39001 tgcgcgcgtg gtggttggct ccaccgcggt gaaatcacca gaaagggtga aaggctggtt
    39061 tgaacgtttc ggtgccgatg ccttagtgct ggcgctggat gtccgtattg acgagcaagg
    39121 caacaagcag gtggcggtca gcggctggca agagaactcg ggcgtttcac tggaacaact
    39181 ggtggaaacc tatctgcctg tcggcctgaa acatgtgctg tgtaccgata tctcgcgcga
    39241 cggcacgctg gcaggctcta acgtctcttt atatgaagaa gtgtgcgcca gatacccgca
    39301 ggtggcattt cagtcctccg gcggtattgg cgacattaac gatgttgctg ccctgcgagg
    39361 cactggcgtg cgcggcgtaa tagttggtcg tgcattactg gaaggtaaat tcaccgtgaa
    39421 ggaggccatc gcatgctggc aaaacgcata atcccatgtc tcgacgttcg tgatggtcag
    39481 gtggtgaaag gcgtacagtt tcgcaaccat gaaattattg gcgatattgt accgctggca
    39541 aaacgctacg ccgaagaagg tgcagacgaa ctggtgttct acgatatcac cgcttccagc
    39601 gatggccgtg tggtagataa aagctgggta tctcgcgtgg cggaagtgat cgacattccg
    39661 ttctgcgtgg cgggtgggat taagtctctg gaagatgccg cgaaaattct ttcctttggc
    39721 gcggataaaa tttccatcaa ctcccctgcg ctggcagacc caaccttaat tacccgcctg
    39781 gcggatcgct ttggcgtgca atgcattgtg gtcggcattg atacctggta cgacggcgaa
    39841 accggtaaat atcatgtgaa tcaatatacc ggcgatgaaa gccgcacccg cgtcactcag
    39901 tgggaaacac tcgactgggt acaggaagtg caaaaacgtg gagccggaga aatcgtcctc
    39961 aacatgatga atcaggacgg cgtgcgtaac ggttacgatc tcgaacaact gaaaaaagtg
    40021 cgtgaagttt gccacgtccc gctaattgcc tccggtggcg cgggcaccat ggaacacttc
    40081 ctcgaagcct tccgcgatgc cgacgttgac ggcgcgctgg ctgcttccgt attccacaaa
    40141 caaataatca atattggtga attaaaagcg tacctggcaa cacagggcgt ggagatcagg
    40201 atatgttaac agaacaacaa cgccgcgaac tggactggga aaaaaccgac ggactaatgc
    40261 cggtaattgt gcaacacgcg gtatccggcg aagtgttaat gctgggctat atgaacccgg
    40321 aagccttaga caaaaccatc gaaagcggca aagtcacctt cttctcgcgc actaaacagc
    40381 gactgtggac caaaggcgaa acgtcgggca atttcctcaa cgtagtaaat attgcgccgg
    40441 actgcgacaa cgacacgcta ctggtactgg cgaatcctat cggaccaacc tgccacaaag
    40501 gcaccagcag ctgcttcggc gacaccgctc accaatggct gttcctgtat caactggaac
    40561 aactgctcgc cgaacgcaaa tctgccgacc cggaaacctc ctataccgcc aaactgtatg
    40621 ccagcggcac caaacgcatt gcgcagaaag tgggcgaaga aggcgtagaa accgcactgg
    40681 cagcgacggt acatgaccgc tttgagctga ccaacgaggc atcggacttg atgtatcacc
    40741 tgctggtgct gttgcaggat caggatctgg atttaacgac ggtaattgag aacctgcgta
    40801 aacggcatca gtgagttgca gggtaagcgg atgcgatgtt gttgtcgcat ccggcaaaaa
    40861 aaaagggcaa ggtgtcacca ccctgcccgt tttctttaaa gctgcaaaga ttacttcgcg
    40921 ttataattac gcagagcgtt acgccccagc acaatccccg cgccaaccat gccgcccagc
    40981 agcaccgcca gaatcaaggt aattgccttt ttcgggctat cgcgacgaat aggtaacgtc
    41041 ggtttcatca cataacggta agcatgaata tcaagatcat caacctttaa gttttcaata
    41101 tccaacaggt tttgacgtgt ctgatagtag tttggtgaga acaccaacgg acgggtcgcc
    41161 tcatgcttaa tcatcgactc cagcgcttcg ctccccaaaa ggaacaacgt atcttgtgtc
    41221 acatcttcag tctgctgaac ctgtggcttt gccacctgcg cctgattcgc atactgcaac
    41281 gcttcctgaa tctgacggat acgcagatct ttctgctcct gcgcgactac ttcctgcgtt
    41341 cttaaagagt cctgcaagtt tttccgtccc agagcaatgt tgtctttgag atccttttct
    41401 agctcttgat tcactttatc atcaacttgc tgaatgtatt gggccaactt catttgtgct
    41461 ccctctgcag tttgcccaac ataagaaaca gtcaatggta attgctggtt cttaacagaa
    41521 ggttcgatgg taagtttttc tggttcttcc tgattatcca gcgtttctgc taatgcagag
    41581 aaggcagaac tgaagcgacc aattaacgtc tcctgtaaat ccgatacttt cggtgcagcc
    41641 tgaccataga taacattcat ggcattgtta tagccagcaa tttgccccac gtcgggctga
    41701 gtgataattg ctgttgacgt ccatttctcc ttcgctactg ccaaatatcc aatagccagg
    41761 gcaatagcca caatgacgga aatgatgatt gtcatcttgc cacgccacaa ctgcactagt
    41821 aaatcaatca aatcaatctg ttccgggtca tggttttgcc cagaaacatt attattttct
    41881 actctcatca ttaccctaac tgacggaaat ttccaagtcc gcatagttta gcgataatcc
    41941 tagtgaatgc tataggataa gtgataaaaa taatgaatta ttggggaggt aacaaaagac
    42001 aatcagggcg taaatagccc tgataacaag atgttagtcg ctgccaaaga gatcgcgggt
    42061 gtatacctta tctgccacat ctttaagctc ttctgccatt cggttagaga taatgacgtc
    42121 ggcttgttgt ttgaagatgg cgagatcacg ttccagccga gagttgaaga atgagtcttc
    42181 tttcatcact ggctcgtaga tgatcacttc aacgcctttc gccttgatac gcttcataat
    42241 cccctgaata gaagacgcac ggaagttatc tgaaccgctc ttcataatca gacgataaat
    42301 acccaccact tgcggtttgc gtgacaaaat ggcatcggca ataaaatcct tacgcgtgcg
    42361 gtttgcatcg acaattgccg agatcaggtt attcggcaca gactgatagt tcgccagtaa
    42421 ctgcttggta tctttcggca agcaataacc accataacca aacgacgggt tgttgtaatg
    42481 gttgccaata cgcggatcga gacaaacgcc ttcgataatc tggcgagtat tcagacctaa
    42541 actttctgcg tagctatcca gttcgttaaa gtatgccacg cgcatcgcta ggtaggtgtt
    42601 tgcaaaaagt ttaatcgctt ctgcttcggt cgaatcggtg aacagggtcg ggatattttg
    42661 cttaatcgcg ccttcctgta acaacgcagc aaaacgttct gcgcgttctg aacgctcacc
    42721 gatgacaata cgtgaaggat ggagattatc gtaaagggct ttaccttcac ggagaaattc
    42781 cggagagaaa ataatatttt cagtacgata tttcttatgc atcgcttcgg taaaaccaac
    42841 gggaaccgtt gatttgatga ccataaccgc ataaggattt atctcaacta cgtctttaat
    42901 tactgattct acactggatg tattgaaata attagtttta ggatcatagt cggttggagt
    42961 ggcgatgatg acataatcag catcccggta agcttcattt ttatctaatg tggcattaaa
    43021 gtgtatttta tctgattgca aaaactgctg aatttcctta tcaacaatag gagatatccg
    43081 atcattcagc atagcaacgc gtgacggtaa aatatctaat gccacaacct catgattttg
    43141 tgcgattaga agcccgtttg acaagcctac atagccagta ccggaaatgg tgattttcat
    43201 ttcatgctct cagaattaac ttaactgtga atcatgatgt ttttagcatc ctgataagag
    43261 ctaaatgttt taacgctacg ttattagctg gtcaacgcta cgaaaaacaa ttcagatttt
    43321 ctttataaga atagctgata tttattaagt taatattaag cagtgaaatt tagtctataa
    43381 gataaatggc aaaaaaaaag cccggtgcaa tatacgccgg gcctcaattt tactgttggt
    43441 taaatcagat taatccagcc attcagtatg gaacacacct tctttatcaa tgcgcttata
    43501 agtatgtgca ccgaaatagt cacgctgtgc ctggatcagg ttcgcaggca gaacagcggc
    43561 acggtagcta tcgtaatagg caaccgcagc ggcgaaggtc ggaaccggga taccgttctg
    43621 tactgcataa gcaacgacat cacgcagcgc ctgctggtag tcatcggcaa tttgcttgaa
    43681 gtacggagcc agcagcaggt tagcgatctg cggattttcg gcatatgcat cggtgatttt
    43741 ctgcaggaac tgcgcacgga tgatgcagcc agcacggaaa atcttcgcga tttcgccgta
    43801 gttcagatcc cagttgtatt cttcagacgc agcacgcagc tgagagaagc cctgagcgta
    43861 agaaacgatt ttgcccagat acagcgcacg gcgaactttt tcgatgaact cagccttgtc
    43921 gcctgctgac tgagcttgcg gaccagagag aactttagat gcggcaacgc gctgatcttt
    43981 cagagaagag atataacgtg caaacacaga ctcggtaatc agcgacagcg gttctccgag
    44041 atccagcgcg ctctggctgg tccatttacc ggtacctttg ttagccgctt catccaggat
    44101 cacatcaacc aggtagttac cgtcttcatc ttttttagtg aagatatctt tggtgatgtc
    44161 gatcaggtag ctgctcagtt caccgttatt ccactcggta aaggtctgtg ccagttcttc
    44221 gttagagaga ttcaggccgc ctttcaggag agaataggct tcagcaatca gttgcatatc
    44281 accgtattca ataccgttgt gaaccatctt cacataatgg cctgcgccat cagcaccgat
    44341 ataggtaacg cacggttcgc catcttcagc aacggcggcg attttggtca ggatcggcgc
    44401 aaccagttca taggcttctt tctggccacc aggcatgatg gacggccctt tcagcgcccc
    44461 ctcttctccg ccggaaacac cggtaccgat gaagttaaag ccttcggcag aaagctcacg
    44521 gttacgacga atggtgtcct ggaagaaggt gttaccacca tcgatgatga tgtcgccttt
    44581 atcgagatat ggtttgagcg aatcaatagc agcatccgtg cctgcacctg ctttcaccat
    44641 taacaggatg cgacgaggcg tttccagaga ttcaacaaac tctttcaccg tatagtaagg
    44701 aaccagtttc ttgcctggat tttcggcaat cacttcttcc gtcttttcac gggaacggtt
    44761 gaaaatagag acggtataac cacggctttc gatgttgagc gcaaggttgc gccccatcac
    44821 tgccataccg actacgccga tctgttgctt tgacattgtt tactcctgtc agggggtggt
    44881 caccgccgag tgtgcggctt aaatgtggta tagatgttaa ctcactcata gagatggtga
    44941 atagcattac ctttgctata ccacactgaa tatctcacct tttccgtctt ttaaaactta
    45001 tttatatgaa attactgaat tacttgttca gtaactcaag aataagtttt gtcttttctt
    45061 ccatcagcgg cacatcaccg cgagattcca cattcaaccg caccaccggt tcggtgttgg
    45121 aggtgcgcag gttaaagcgc cagtcggcaa aggtcatgct gatgccatcg gtgcgatcca
    45181 ccgccagcgc ctcgcggcta aaatgctgtt ccacgcggtt aatcgcctca aagggttgcg
    45241 ccagtttgct gttgatctca ccgcttgccg gaaacgctgc catccggtcg cgcaccagtt
    45301 cgcccagcgt ttttcctttc aggcacacca gttcggcgac cagcagccac gggatcatgc
    45361 cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc gctcatttca ccaccgtaga
    45421 tggcgtcttc cttgcgcatc cgttctttaa taaaggcgtg tccggttttc gacatcaccg
    45481 gcgtgccgcc tgcggcagtc accacatcaa cggtgttcca ggagagacgc ggatcgtgga
    45541 tgatcttcgc gccgggattt ttttcgagga atgcttctgc cagcaggccg acaatgtagt
    45601 agccctcaat aaactgccct ttttcgtcaa acaggaagca gcggtcaaaa tcgccatcaa
    45661 aagcaatgcc catatccgca ccgtgtttga tgaccgcatt gcgggtgtcg tcgcggcatt
    45721 ccggcagtag cgggttagga ataccgttgg ggaaattgcc gtccggcgtg ttgtgcactt
    45781 tgattaattc cacgggtgcg ccaagggctt taaagcgggc ttcgatggcg tccaccaccg
    45841 gacccgctgc gccattcccg gagttaatca ccagcttgag cggcgtgagg tttttgacat
    45901 tgatataacc gaacaggtga tcaacgtaag cgtcacgcag attgatttgc tgatagcgac
    45961 cgcgtttggt ttcatcaacg ggaggaaagt cgttggcttc tgccagacgc tggacatcgc
    46021 gcagtccggt atcaccgctg atcgggcgag ccccttcgcg caccagtttc atgccgttgt
    46081 aatccatcgg gttatggctg gcggtaactt caatgccgcc atccacgccg agatggaacg
    46141 tggcgaaata gatctcttcg gtgccggaca tgccaatatc cagcacatcg acgcccgcat
    46201 cctgtaaacc tctcgccagc gccagtttta aggtttcgct ggtgaggcgg acatcaccgc
    46261 ctaacacaat ggttttcggt ttgagaaatt cgccataggc gcgaccaatg cgccaggcga
    46321 tatcttcatt cagttcttcg cctaatttcc cgcgaatatc ataggcttta aagcaggtta
    46381 attttttcat gtcgttaccc tttttcaggc aatagttggc cctgaccgaa acggccaatt
    46441 ttgtttttgt cgttatttac atctcggttt cgcattcatt gcctgatgcg acgtttacac
    46501 ccgtccgtag cgatccgcga aacgcaccac atcatcctct tcgagataag agccggagcg
    46561 cacttcaatt aaatcgagcg gaattttccc cgggttttcc aggcaatgca tcgcccccag
    46621 cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa tggtgacttt
    46681 tgccgttccc gcgacaacca cccagtgttc cgcgcggtga tggtgcatct gtaccgacaa
    46741 gccctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg cgtcgataga
    46801 gtcatatttg ccccacgggc gatagacttc gcgatgcacc cgatgctcat ggcgaccatc
    46861 ggctttgatc tgctcgacca cttttttcac atcctgcacc gcgttacggt cggcaatcag
    46921 caccgcatct ttggtctgca ccactaccag atctttcacc ccgacggtgg ttacgaggcc
    46981 agattcggcg tacacatagc tgttttcagt tttgtgatta atcacatcgc cgtggcaaac
    47041 gttgccctcg gcggtgtggg cgctaatttc ccataatgag gaccaggagc cgacatcgct
    47101 ccagcccgca tccatcggca ccacaacggc atctgccgta cgttccatga ccgcgtaatc
    47161 caccgactct tccgggcagg cgagaaacgc ttcttcatcc acacgaataa aatcgagatc
    47221 cggatcgacg gcgctcatcg ctttttcaca ggcatcgaga atatccggac gatatttttt
    47281 cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc agtaatattc
    47341 gccgcttgcc acataggcct gggcggtttc cagattcggt ttttcgacaa actgcgccac
    47401 ttcaaaggcc accgtatcct gctcacccgc agacacttca ccgcgacgaa tatagccata
    47461 accggtttct ggtagatccg gcacaatgcc gaaggtcacc agcttgcccg cttcggcata
    47521 tggcatggca ttacgcacgg cggcacggaa cgcgtcttca tcggcaatca catgatccgc
    47581 cgccaatacc agcattaacg ggtcgctctc cgggctatga cgttttgccg ccagcgccgc
    47641 cagcgcaatg gcaggtgccg tgtttcgccc tgccggttcg agaataatgt tctcggtaag
    47701 tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat tgcaaatcac
    47761 caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca gcatggtgag
    47821 atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg gccataagcg
    47881 gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca taattatccc
    47941 cgaatatcat ttataaattg acgtaacacg ttctctttat cgagtgtgcg ttcggcatat
    48001 tcacgtgcca ccgtgttgtg tttgggcagc aggagcgcct gacggatccc cgccaccagc
    48061 gcctcgaccg attccggttc tacgcaaacc gcaatgcccg gaaaggtttc gcaaagctgc
    48121 cccagttctg tgtgggcctc tgcggtaatc accgcgttac cgcctaccgc cagaatattg
    48181 gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt tttgcaccac cagatggcaa
    48241 tcgcccatct tcagcagtgc gggtaaagcg tcatacgatt gcagcgggaa aaattgcatg
    48301 ttgcgcagtc cacgctgctg cgccattttt tccagccgcg ctttgccgcc gccctgcccg
    48361 acaatggcaa aaatcagcgg ttcatcgcgc agacgatcgg cagcttcaat aacgttttcc
    48421 agcccctgct tttcaccaat attgccggag taaagaatga tttttttgtt atccggcagg
    48481 tcaagctggt tacgaagggc gtcaacatcg gcatctgcaa catgctgaaa acgggcaatt
    48541 tccgaccagt tggggaagaa gatgacgttt tccgccgcca cgcctttttc gatggcttta
    48601 ttcatcatcg aacgcgaaat cgtggagacg ttatcgacgt tatgcagtcc gctacgttcg
    48661 aacgccgttg ccagctgtgc cactttgctg cctttgcctt ttccggcaag gcccagcccc
    48721 agcatggcgt ccacttcgta atcctgaata tgcagcacgg tacgcgcacc agaaagtttc
    48781 gccagcaggc gcattcccgg cgtgcaaaac agcgttggca ccacgccgat aatgcgatcc
    48841 ggcttccagc gacgttgcgc catcagagga aagaaactgc tgacggcaaa actgccgaga
    48901 tgtaacaggc gtttcagggt gctcggctgt tttggcacgt acagcgggca gcgccacacc
    48961 gtggcggccc cctcttctcg tttgtaccgc caggcggaat agttctcgcc cacctgccac
    49021 tgcgggtagt aaggcggtgc ggtaataacc cgcacctcat gaccttgtgc cgccagccat
    49081 tccaccatct cgccggtgta tttgccgata ccggttaact ccggcgagta gttaatgccg
    49141 tagaccagta ttttcataat ccgggtactc cggcacgctt ctcagcgaga aaataggcgc
    49201 ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc cggcgtcagc cagcggtaat
    49261 cgtcatgctg ctcatccggc agtatcagct cttcttcagc gactctgaag cgaaaaccga
    49321 gcaccacata gtgagtggtg aaatccgtgc cggagaagtt atcgtcataa aagtgctgcc
    49381 agacaccgta aaactggcct gctgttatcg gcagacgcag ccccagttcc gccatcgtca
    49441 gccgctcgaa tgcggcttcc agcgtttcgt ctttctgcac gcgccctccc ggcacaaacc
    49501 agtaaccctg cgccgggcgg ttggttcttt tgccaagcag aaactcaccg cgactgttct
    49561 cgacaataaa gtcgagagag acaagcgggg tggagcgcac taccgtggca aagtcttcct
    49621 gacgtaaaaa catcattacc cccgaaagcg gtcttgattc tcaaggaacc actggtaagt
    49681 gctggcaagc cccgcttcca gtgagatttc gtgataccag ccaagctgat gcaggcgcgt
    49741 cacatccagc agtttgcgcg gcgtaccatc cggtttgctg gcatcaaaaa ccacccgacc
    49801 tttgtaaccc accactttgg cgatggtttg cgccagctcg cggatagtgc agtcaacgcc
    49861 cgtgccgacg ttaatgtgcg acagcatcgg ctgggtgttc tccagccaga cttcgtgcgc
    49921 cagctccatg acatgaatgc tcgccgccgc catatcatcg acgtgcagga attcacgcat
    49981 cggtgtaccg ctgccccata ccaccacgtc cggtgcattc tgtgccgtcg cctcgtggaa
    50041 gcgacgcagc aatgcaggga tcacatgcga attactcggg tggaagttgt catgcgggcc
    50101 atacaggttg gtcggcatga ctgagcggta atcgcgtccg tactggcggt tgtatgattc
    50161 gcacagtttg atcccggcga ttttggcaat ggcgtaaggc tcgttggtcg gctccagcgt
    50221 gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc agtttcgggt agatacagga
    50281 cgatccaaga aacagcagtt tgttcacgtc gttctgatgc gcggcgtgaa tgatgttgct
    50341 ctcaatcatc atgttctggt agatgaaatc cgccggatag gtgttgttgg caacaatgcc
    50401 gcccactttc gccgccgcca gatagacctg gtcaatgcgt tcgctggcaa agaaatcatg
    50461 caccgcgcgg ctgtccagca ggttcagctc gtcgcgggtg cgtaatacca gttccacatc
    50521 accgcgctgt tcgagctgcc gcctgatggc agaaccgacc atcccacgat ggccagcgat
    50581 aaaaatgcgt tgtttactca tgcttatgac tccagcgcga tcgccacgtc gtagccgtga
    50641 gatttcagca gagagtgttt tttcgccgct tcgaggtcat tagccaccat ttcagacacc
    50701 atctctctga gggtgatttc cggtttccag cccagttttt cgtgcgcttt ggtcgggtcg
    50761 ccgagcagcg tttcaacttc agccggacgg aagtaacgcg gatcaacggc gataatcaca
    50821 tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg aaaccacaat gcccttctct
    50881 tcaacacccg tgccttcaaa gcgcagtttg atgcccagct gtgccgctgc catttccacg
    50941 aactgacgca cggagtactg aacgccggtc gcgataacga aatcttccgg ctgttcctgt
    51001 tgcagcatca tccactgcat ttttacgtag tctttggcgt ggccccagtc acgcagggaa
    51061 tccatattgc cgaggtacag gcacgactcc agcccctggg cgatgttggc gattgcgcgg
    51121 gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg attcatggtt gaagagaatt
    51181 ccgttacaag cgtacatgcc gtaggattcg cggtagttaa cggtgatcca gtaggcgtac
    51241 agtttggcga ccgcatacgg agatcgcggg tagaacggcg tggtttcttt ctgcggaatt
    51301 tcctgcacca gaccatacag ttcagaggtg gaagcctgat agaaacgagt tttcttttcc
    51361 agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc gacgtcagcg
    51421 gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcccag gttgtacact
    51481 tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact caggtcgcca
    51541 taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg atccacgcgc
    51601 tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc tttttccagc
    51661 agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag agcgactttt
    51721 gacatgtctt attcctctgt atttttgaat ttattcagtt tcaacgcgtt cgcgtatcac
    51781 cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa cactgcttcg
    51841 tgcacccacg acggtgccgt cgccgattgt gacgcctggg gcaacaaaga catcggttgc
    51901 cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat gttgacttgc
    51961 atggtcgtgg ctaccggtgc ataaataact tttttgcgat atcaccgaat gtgcgccaat
    52021 ggttatttca ccgagggtat ataaattgac gtcatcgccg acccacgcgt aatcacctaa
    52081 ggttaatttc cacggatagg taatttttac tgacggacga ataactacgt tttttcctat
    52141 ttttgctccg aataaacgta ataaaaaagc ccgccagcga tacaatactt gtggcgacca
    52201 ggcaaatatt gttgcctgta ctgcccacca taattgcact ttaatagcgt tgccgccccg
    52261 gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt ggttttcctt atattcagac
    52321 tttgttatat aaggctttcg tctttgaggt agtacgttgg cgtaaatgcc aggataattc
    52381 agcccaaaag ccaggcacat gtaatatttg tcgttggact tttttcgcgt cagcacacaa
    52441 ttccatatta ttggtggtag ataccccacc catggaaaat tcagacacca ggccattgag
    52501 ttttttaaat gcataaccgg ctttatacat tttggctgcc agcgcgtagt cggaagaaac
    52561 tttatattcc aggtcataac gccatttttt caagccggat acagggaaaa atatcgcctg
    52621 atgactggcg ggcaggctgt gataaatata ccagcccggt ttggcgctac gtttaatttt
    52681 atgcccgtcg ccaaaatcca gcagcgcatc gccggtgatc atcatgttat ctttttgcat
    52741 ttttaactta cggacaaaat ttgcggcatc ctgatgaaaa atatcgcccg aattgagaaa
    52801 caacgcgaac ttgccttgtg ccatcgtaat gcctttgttc atggcgtcgt agataccgtt
    52861 atctggctcg ctgacaaagc gtaggttata gataccattg agattttcca gatactcacg
    52921 ggtgccgtca ttggaaccgc catcgacaac aatccattcg aagctgatat cttccgcctg
    52981 cgccagatgc gccagcgagg catgtgtttt gactatccct tcgaggttac gaaacgcgac
    53041 agtgattatg ctaagcaaca tgttcttatt cttacctcgt aatatttaac gcttttcgca
    53101 aaataaacgg acagacgatt aaaaatgcat attccgggct aaatatcgaa ccggtaaaaa
    53161 acagcgatac cggagtaaaa agatataatt gcacgcgaaa attgcggtta tcaccaaaag
    53221 cgttgatcat catttttatc actttcccca tgtaccacag cgataaaaac actgcgaacc
    53281 aggaaaaata aataatcagc agatacaaac cattgtctat ggtttttccg acatccgcac
    53341 cgttaaatat tccgaatgat gcgacgtatt cataaagtga gccaaatctg actacaccgt
    53401 caatatgggt caaagaataa ccgaccatca ccagcggacc gacaatacga taatacgatg
    53461 acgatccttc cgtacctaaa tcgcccagac gggtggaaat ataaggaaac gcgattacca
    53521 caccaacccg gaatacagcc agagaaatca atgccaacgg taactttttc ttaatcgcct
    53581 ctttattcag atattgaaat gcccactcca gcaaataaaa caggataaaa gtcataaccc
    53641 ctgaaaacga tccggataat attatccctg cgagaatcat agcatcggtt ttaggcgttt
    53701 tgataccaaa ctgtttgatg ctgagccaaa ttgagattaa tgccagagcg aaaaatgccg
    53761 gttcgaaata cagtgctgtc gtgcgcttgc cgccgaattt aatgaaattc agtacatagc
    53821 tgttgctgta aatcagatat ttcgaaatcg actccataat actgctgcca ccggtgagga
    53881 taatttgcgc catctccacc gccgccagcg ccaccaccag cccgatcacc agataaaaga
    53941 aacgtaatat cttgcgatgg ttgtgtggcg aaatcgtttt aaagcgaata ctccacacca
    54001 tgccaataat gatcacaata tagacaaaca gcatggttga agtgacgtat ttactggcat
    54061 ccagcgactg accaaacaga tagttaaacg ccgtgagtcc cgcaccaatc cctaaggcaa
    54121 tcatcaattt tttaacgctg atgcgttcta aaaacagcag taacaagaca ggtaaaaaag
    54181 tgacgatggt gatggggaaa ctttcgccaa gctgggcgat tttgacgtta accagcaggt
    54241 agatcagcgg cagcagcagg tagctacaga ttctgataga agttgacata ctcctccagc
    54301 atctgttgtc cactgtaggt ggcgcggctg cgttggctga actcagccag cgtggtacca
    54361 aatatcgcct gcgcgatttc cggtttgctt aactgcacca gttgcagcac gtcttcttcg
    54421 tcgacggttt taccgccgga tttttgcaac acttcccgtg ccgcgtcgct atgggtggca
    54481 atcaccggca cgccaattga taacgcctca cacaaaatca gcgggtagtt atcgacgcga
    54541 gaactgaata ccagcgcatc catctgattg agcgcgctca tcaacttgcg cttgtcagtt
    54601 tcaaagccgt gattaaccac gttgccagcg gtgaacggcg agaacttacc aaaggtatgc
    54661 agttcgattt tgtcgcccag cgccatcatc tcacgcacca gttgctggtt agtcttgccg
    54721 tcgtaacgca agtcatgcgc caccaccgcg attttcggct tgccctgggt ttcgcgcacc
    54781 ggaggcaagt ccgccagaat cgcttcggtt gccatatcaa taccattatt gataatccgg
    54841 caacgccctg gaccgtacag gctattgaaa gcgtcagcca catgctggct gggggaaata
    54901 aactgacagc ccagcgccag catctcacgg aataactggc gtttgcccgc caccagctgg
    54961 tgtgcgcgat caatcttcac cggcggatag ttatttaagg tcgggcattt ctggcagccc
    55021 gttttccagc cttcgcaacc gtcggtaaag gcgcagcgtc cggtaacact ccagtggtcg
    55081 tgcagcgtcc agaccagagt gacgtccggt ttatggtttt tcactttttc gcaaaagcgc
    55141 accacgctct taagatttag ccagtagctg tgcagcacat gaaaatgcag gaccaccgga
    55201 cccggtgtgc gagtaatggt gcgatataac tcattgaaat tgccaaacag atcgcgatta
    55261 aacagacgaa acagggcaat attcgccatc gcggtcatcc gcggcgtatg tttgatgacc
    55321 tgcggatagt tctgatggct gacgctctct ttgccgcctt tgccataacc gtagacaaaa
    55381 tgtgacgcca gcccctgttg cagcgcacgc tggtggagat ctaacgccac acctgctgcc
    55441 ccgccttccg ccagtcgcac attaaattgc agaatattca tttaattacc ttcactcgcg
    55501 ctttttctcc caccaccagc gcgttgtccg ggacagagtc gagcaccacg ctgcccgcac
    55561 ccacggtgac gttgttacca agcgtgatat caccaagaat aatgacgttg gcaccgagtt
    55621 cgacgccgtt gccaatgtgt ggacatgcca tgttatcggc accacgattg ccgatagtga
    55681 cgccgtggcg aatggtgaaa tcatcccccg ctaccacgtt tttattgatc actacggcgt
    55741 aaccgtgatg gatagtaaag cggcggccaa tggtcgcggc ggcctggatt tcataaccga
    55801 aaaagcattc ggtgataatg cgatacagca ccagcagcgg ggccgcccac agattgttga
    55861 ggacgttctt tttgcgccac accgaacaaa aatgagcaac acgataggca agaaccatgc
    55921 agcacgggcg taaactccag ctgttggcac gcagatcttc cagcatcttt agcgcccccg
    55981 aataccatca gccagacgtt tgccgttacg caccgacagc agcgtcagca aagtgcgcca
    56041 ggtcatacgt ttattgcgga tctggtagag ggtaaacagc tgatattttt tgcttgcacg
    56101 gtcaaattta tctttgtgct ttcggtaaaa gtggaagtac ccggagaatt ttttcggtga
    56161 cgaggtgatc tgcatttcac cgtgattgat atgcaggatc tgcgtcgctt cttccacttt
    56221 ccacggttcg ccgtactcca ccaccatccg caggaagatg tcgtaatcct gtgctgcttt
    56281 cagttcggta tcgaacaaac actctttgaa acgccatgcc caagtaaaga cctgattgcc
    56341 aatgatattg cgtttataga acaggcggcg tgagtacggc gatttgggat acagtggtaa
    56401 gcttgccggt tgggaataaa cttctccctg gcatacgtag tcgttagcgt acaaaaaggc
    56461 gtgagtgacc agttgctgtt tatgggcgag gaagacgctc agacggttgg gtgtccattc
    56521 gtcatcgtca tcgatcccgg taatgtattc cccttgcgcc agcataatcg cctggttacg
    56581 taccgcgcac gccccgctgt taatgtcgtt gtgaatgtag gtgatacgcg gatcgttgag
    56641 ggcagtgacg tactgttgca gttgttccca ggaggtggag caatcatcca cgatgatcat
    56701 ctcccagttg ctgtagtcct ggcgcaggac cgattttatc gcccgaatcg ccagttgttg
    56761 acggttccag gtcggcatat agattgaaat cagcggattc tctttcatag gttgttctcc
    56821 cggcttatga gctggtttgc ttgccggatg cggcgtgagc gccttatccg gcctacgggg
    56881 cggtgcgaat gcaggcgtcg ttatttcgca tccgacttat attcgtattc gtaatagcca
    56941 taatcctgat acgcgctggc gcggcggaag atggagttca gaatcacccc tttcaccgga
    57001 ataccgtttt gctcaaagcg gctcagactg gtttccactt ctttcaatgt gttgaccgca
    57061 tagcgcgcca ccattaacgt ggttccagcg tgacgcccga caattgctgc atcggtcact
    57121 gccagaatcg gcggcgtatc aatcaacacc aggtcgtagt ttttactcgc ccagctcacc
    57181 aattcggcaa agcgttcgct catcaacagt tcagaaggat ttggcggcac ctgaccgcgc
    57241 gggatcaggt caaatttggc aatagaggtc ggtttagctg cggtagtaat atcgccctgg
    57301 ccaatcagaa tttccgacag gccattgacg ttattggtac ccaacagctc gtgggtgtag
    57361 cctttgcgca tatcgcagtc gatcaacaac acccgtttat tggtctgact gatcaccgcc
    57421 gccaggttgg cgcagacaaa ggttttaccg attgacgggc tcacccctgt catcatcaac
    57481 acattgttct gcgcctgcat catcgcgaag tgcagactgg tacgcagact gcggatggct
    57541 tcaatcgcca gatcggttgg attccccacc gccagtagct ggctctgttt atagcgttta
    57601 atccctttga tggttttgac gctatcgcgc gctttctgcc attccgacag cgggatgctg
    57661 gcatagacgc tgataccatg ttcttccagc acctgcgggc tttcgatgcc gcgattaaac
    57721 aacgagcgca gcagcacacc cacgatagag agcatcaggc cgaggataat tgcgccgagg
    57781 ataatcagtc ctttcttcgg tttcagcacg ccaggctggg tgattgccgg gtcaacaatg
    57841 cgcacatcgc cgacggtgct ggcttcggtg attttcagct cctgctcttt attcagcagt
    57901 tgcatataga cctgctgacc agactcgaca tcgcgggtca ggcgcacaat ctcctgctgg
    57961 gttttcggca tcgccgttac gcgaccgtta agtttggctt tctcttcttc cagcgcctga
    58021 cgtttctcca gcagcgtgcg gtacgccggg tgaactttgg tgtacagctt ggagatttcc
    58081 gcctctttaa aggtcagttc gttcaactgc gcgtcgatgt tcaccatcga atcgagcacc
    58141 gctttcgctt ccagcggcag atcaacagaa tctttatcct gacggaaggc attcagtttg
    58201 ttttcggcaa catcgaggcg gctacgtact tccggtaact gttgcgcgag gaaagcgagg
    58261 cttttcgacg cttccgccga tttgcgctca atattttgtt cctgatagtt acgggcgatg
    58321 ctgttaagaa tgtcgcggat ctgttcgcga tcttcaccgg tataagtcag gctcagtacg
    58381 cctgcgtctt tgccgttctc cgttaccgtc aggctgtttt gcagttggtt gatcatcccc
    58441 aacgtggagt atttggtgac ggtaaactca ctgcccgggc gggcgtgaat ggcttcaacc
    58501 atcagcgtga cgccttcttt tttcagcatc tggcccgctt gcccacgggc gctaaagccg
    58561 ccatcactgc tcagggtgta gtttttattg tccagcacat taagcgtaaa cacctggccc
    58621 gccatctctt tcgggcggtt aaaggtagtc actttcaccg tctcgttctg gcgtcccatc
    58681 agtcgctccc agcccgcccc gaaaatcggg aacgtgtttt tgctcactgc aatatcgagg
    58741 tcgagatcgt ccaccgtttt accaagcacc agacgcgaac gaatcaactg gatctcggcg
    58801 tccgatgcag gcggtttgtt ggctaatgcc gaaccaatgt cctgcactaa cgaattgccg
    58861 ctgttttgct cgatttgtac cagtgcgtcg gcactataaa tcggcgttgc gaagaaggtg
    58921 taaaccacgg cacagagggc aaatacagcg gtgatgccaa tcacccacca gcgcgcttca
    58981 atgacggtgc cgaccaggcg accaatatcg atttcatcac tgcccgttac cggagcggca
    59041 tgttgtttta ctttttctgt cattcttata cctgctctgc gttcaatgcc tgcgcccact
    59101 ggcgggcaga ccgttcaagt aatgtgtaca ccgctgcaaa cgtttcccgg cttttgcgat
    59161 acggatcggg gatttcacat tcgttatccc agtgaccaaa cagcatcact ttgccgcgca
    59221 tctcaggtgc catctcgcat aagcgttcga tatggcgctt ttccatggtc aaaatcaggt
    59281 catagttgcg acacaggcgg cggctgattt gtcgtgcaca gtggccttcc agagacagtt
    59341 gatgttctgc ggcgacgctg atagcggtag gatcagcgcc cttaccgacc agcgcgccga
    59401 gtccagcgga ctccactttc agttccgggt gataacgttg cagtaagcgt tccgccgtcg
    59461 gggaacggca aatattgccg acacagacaa ctaatatgtt gttaaacatg acgattacca
    59521 gttatgaatg tcgctggctg tatccgtcat gtaacggaca ccgctaatag ttggcaacaa
    59581 ctgattgatc agacggttcc agcgggaaac cggggcggtg gtgacataca ccacgtcata
    59641 aggttgcagg cggaattctg tcgccatcac cagcgacgtg gcatcggaca tatccagctg
    59701 gtagatattg gcaatcttgc cattacgccc gccctcgcct ttcagcggac gaatgacaaa
    59761 gatgccgctg gcgttggagg tggtcatgtc gataccttca gcattaccca gggcttcagt
    59821 cagggtcatg ccgctaaagt ccattttcag ggtgctctgt ttcttcactt cacccatcac
    59881 aaatactttc agatcatcat tacgcggcac gtagagaata tcgccgggat aaagcagtcg
    59941 gttctgattg aggtcgccgt tttgcatcag cgcctgcaaa gaaatgcgtt cttcacgacc
    60001 attgtgtgtt agcactacgt tgcgccagtc agcggtgtcg gtcaggccac ctgcggcgtt
    60061 gatggcgtcg agaatagtca gtggcacgtt ggtgatcgcc tgttgaccgg atttattcac
    60121 ctgaccggag atataggcct tttgtgagcg gaaggcggcg atattaacgt ccacctgcgg
    60181 gtcagcgatg tacgtcgcta agcgcccggt aatatcactg cggatttcag cgagcgtttt
    60241 cccgactacg tgaaccttgc cgatatacgg gtaaaacata gtgccgtcag gctgtaccca
    60301 gttgccggtg tcgctggagc tgcggtactg accggctggc gtggtgagtt ccgggtgatc
    60361 ccagacggtg acattcagaa cgtcgcccgg cccgacgcga tactggtaat tcgcgatctc
    60421 gctttccagc gtcatattgg ggcgcgctac attcgggcgt gggcgtaatt ggtcaatcag
    60481 gcgcggggtc agcggataaa cattcaccat tttgtcgaga tcgaaatcag cgtcctgctg
    60541 tttgatgacg tctttgccca tcgtcgacat attgctgccc ggaagtactg tgcaaccgct
    60601 tatcaaggtt actgacacca ataatggcat caatttcatt ttggatttca tcattgttta
    60661 tttatcactt tggcagagta attatcctgt gcactattaa tagcaatatc gccatgcaca
    60721 tttaccttgc agttaattga ataaaaattt aactggcatc agtcctaaaa aaattgattt
    60781 catccgcagg ctattgacag aataattcag actggtcttt caggcatcca gacacgctac
    60841 cgcccctggc tttttagcca ccaacacact gatttagctt aatttttcaa accctctctg
    60901 catacaatcg ttgatgagaa agggttattg cggaaattaa cttccgaata taaggtgaca
    60961 ttatggtaat tgaatattgg ctctccaata atgcaggagg aagtgttaca gctaacggaa
    61021 tagcaggcaa ggtaacaatt cggcaattgg ctatttttaa gaattatatt aagtgtcatt
    61081 caatatggtt tttaggagtt tctttaggtt gacaatattt aatattgcgc ctccacatgc
    61141 gatatttctt aaataatgtt ttattattac cacttttaat tcagggataa tgtgaggtta
    61201 ttaccctcaa ataatatgag ataaatagtg ctgcaacatt gcatttttgc cccgatttat
    61261 ccatgatcga attgtgacat ttgtcataca acgaataggt tttgtactta ctatggaatg
    61321 gattgccgat ccgtctatct gggccgggtt aatcacgctg attgtgatcg aactggtcct
    61381 cggcattgat aacctggtct ttattgccat cctcgccgaa aaactaccgc cgaggcagcg
    61441 cgaccgcgca cgggttaccg ggctgctgct ggcgatgtta atgcgcctgt tactgctggc
    61501 gtcaatctcc tggctggtca ccctgactca accgctgttc agcttccgct cgtttacctt
    61561 tagcgcccgc gacttaatca tgctgtttgg tggtttcttc ctgttgttca aagccacgat
    61621 ggagctgaac gaacggctgg aagggaaaga cagcaataat cccacacaac gcaaaggtgc
    61681 gaagttctgg ggcgtggtga cgcaaattgt ggtactggac gccattttct cactcgactc
    61741 agtgattacc gccgtcggga tggtcgacca tttactggtc atgatggccg ccgtggttat
    61801 cgctatcagc ctgatgttga tggccagcaa gccgttaacg caatttgtta acagtcaccc
    61861 gacgatcgtt attctctgct taagcttcct gttgatgatt ggctttagcc tggtggcaga
    61921 aggtttcggc ttcgtcattc cgaaaggcta cctgtacgct gccattggtt tctcggtgat
    61981 gatcgaggcg ctcaatcagt tggctatctt taaccggcga cgttttcttt ccgctaacca
    62041 gacgctgcgc cagcggacta ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga
    62101 tgcggaactg gacgccgaaa ccgcgtccat gctggtggat catggtaacc agcagatctt
    62161 taatccgcag gaacggcgga tgattgagcg ggtacttaat cttaaccagc gtaccgtcag
    62221 cagcattatg acgtcgcgcc acgatattga gcatatcgat ctcaacgcgc cggaagaaga
    62281 aatccgccag ttgctggagc gaaatcagca tacgcggctg gtggttaccg acggtgatga
    62341 cgcagaagat ttgctcggtg ttgttcacgt tatcgacctg ctacaacagt ctctgcgcgg
    62401 cgaaccgctc aacctgcggg tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc
    62461 gttgttacct gccctggagc agttccgtaa tgcccgcacg cactttgctt ttgtggtgga
    62521 tgagtttggc tcggtggaag ggattgtgac attaagtgac gtcactgaaa ccattgtcgg
    62581 taacttaccg aacgaagtgg aagagatcga cgcccgccat gatattcaga agaatgcgga
    62641 cggttcctgg acggcgaatg gtcatatgcc gctggaagat ctggtgcaat atgtgccgct
    62701 gccgctggat gaaaaacgtg aatatcacac cattgccggg ctgttgatgg aatatttaca
    62761 gcgtattcca aaacctggcg aagaagttca ggtgggggat tatttgctta aaacgttgca
    62821 ggtggaaagc catcgcgtgc agaaggtgca gattattccg ctgcataagg atggcgagtt
    62881 ggagtacgag gtgtgatggt atcggtcatt ttacccaata gccgaaagga ataagatgag
    62941 ggaaccgcgc cagcggcgat ttaccgccgg gagcccgggt tgccagggtg gtggcgattg
    63001 agccaccctg gcacgttcac cggcttgagc gttccatagt agcgaggaac atcaagtgaa
    63061 cggaacagcc ttcgatctta catattcaga atataatgat gccgccacga acgcctgatg
    63121 cgacgcttta cgcgtcttat caggcctaca ggactggtca cctggtaacc aacatcttct
    63181 ccagcaactt cttcacatcc ttgccattgc gggaatcttt attccgctcg gcccagtcat
    63241 tcaggcgacg tttcgcttcg tcctgtagat gtttgcgcag taactgatcc acttgcaggc
    63301 tgtaattgag ttgctgccag ttgccataaa cccgcagcgg tactggcgtt tctttcagga
    63361 aatcaatcag tttactttcc ccgttccagc cacccacgac ccgaatatca aactgggtat
    63421 cgcaggtttg atctgccaga ttcaacatgc cttcccctgt cagcgccagc acaggcgatt
    63481 gaccttgcat gtcgtttaac gtcacgacgc catccttcaa cgtcaaatcg gtggtaaagc
    63541 ggtcaagccg cgtcacgtta tcaaagtttt cagcggcctt cacatcaccg ccattacgct
    63601 ctaccgcttg ctgaatcatc tgctggaagt tcatcccttc catgcgcgtg tcggtcattt
    63661 cgacatgtgc ctgtccttgc cagttgtggc ggaatgcgtc ggcatctatg tcagcaccgg
    63721 agaaatcacc agccagtgac atttttccgg tcaacgaaat cggatagtta aacgccttca
    63781 gaatggtacc aatctcaacg ttttccagcc gtggctggaa gtttatccgc ggatttattg
    63841 atgtcgcgtc cagcgtgccc ggcagtgaaa cctgtccacc gttaagtttg ccctgcagtt
    63901 gagtaatttc cagcaaaccc gacttgttgg tcatttgcgt ggcaacatct gtaaaattca
    63961 ttccgcgcca gcgcacgtta ctggcctgca acaaaatatc agccgtaaag ccttgcagtc
    64021 cctgataggc cggttcatca atacgcgaag aaatgaccgg gcgcggcaac gttgattggc
    64081 tctgcccctg ctgcgcggca ccgttttcac cattcgcggt ttcattaagc gggatgaggt
    64141 tgtcaagatt cagttgcggg aattgcagtc tcagctgcca ttccggtttc tctgtcagcg
    64201 tgacctgtgc ttgcccgctc agcgtactgt cattggcggt caagctgatt tggttaaatg
    64261 aaaggcgttt atgtgactcc tgccactggg cctggaaact cccctgcccc tgaatacctt
    64321 gttttggtaa atcggcaccc tgcaactgcc agttaatttg ttcaatagcc gccgttaaat
    64381 catgcggata atcagaagca tccaccgtac cgttaaggga tattgtgaga tcgcgctgat
    64441 cgcgattaac ccgcccggag aactcaaatg agccacgatg ttggggatct tgttccattt
    64501 gcaggcggat attgcggatt gtcacctgct cgtcatcttc atgctggaac accagcacgc
    64561 tgtccgccac cttaagactg gatatatcaa acgaccatcc gcgatcgtct gacagatccg
    64621 gcaaggtatt gtcgcgcggt gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg
    64681 gcgtcagttg gatcactgcc ccttttagca tcacctgctt aacgctcagt tgatgactca
    64741 gtagtggtaa aagcgccacg tccagacgca tgttgtcggc gcgaaccagt ggctggcttg
    64801 cgccctgggc ggtgagagac attcgcccgg agaggatact aagctgcggc cagacgtgcc
    64861 aacgcagtgg cccgtcgagc tgcaattgat aaccgctacg tgcagcaact tgcttgacca
    64921 tatagtcgcg gaaatcattc ggattcacca gcaacactaa cacagataac ccggccacca
    64981 gcacgaccag gagtatcatc agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc
    65041 tgaattagtc tttatcgatt cggctggcta ccgcgccctg ctggttgcga tatttcgcat
    65101 cttcacggcg gttgtaaggt cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac
    65161 caattaacat gcccggacgc agcgccagcg gcagcttacc ggagttgtag aactccagca
    65221 caatgcaacc agaccagccc ggatcgatac ggtgcgcggt gacgtgcacc atcagcccca
    65281 gacgcgccaa agaggaacgc ccgtccagcc agcccaccag atcggctggc agcgtcaccg
    65341 actccagcgt caccgccagc gccagctctc ctgggtgaag atagaacgcc tcgccctcgt
    65401 cgagaacgat ctcatcgctc atcacgcggt caagcgcggc gctcacttca tctttgggac
    65461 cgctcagatc gataaacgct gccgtgtgac cacggaaggt acgaaattta ttgcccaggc
    65521 gtacatccac cgtcgcgccg ttaatacgct ccactggcgg acgtgggttg atcgacaaac
    65581 ggccttcatc aagccaggct tcaatatctc ggtcacacag acgcatggca ttttctcctt
    65641 tcgcgcatca ctcccttaac gccaattacg tcaagggcat accaggttat cactgaacgg
    65701 tacacaattt atcaagctta ttcaaagaac tgacttattt tcgctttcaa tatatcgatc
    65761 gcgatgcggt ttttcccgcc gcgcggcacg ataatgtccg catattgttt agaaggctca
    65821 atgaattgca ggaacatcgg gcgcacggtt ttttgatatt gcgccatcac ggaatccatt
    65881 gaacgcccac gctcgttaac gtcacgcttg atgcggcgca tcaggcagat atccagcggg
    65941 gtatcaacga aaatggagaa gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa
    66001 atgccttcga gaatgatgac cttcttcggc tcaaccgtca ccgtttcttt catacgcgta
    66061 tgttcaacat agctgtaaac cggcaggtca attgccgagc cgcgtttcaa cgcttgtaaa
    66121 tgctcaagca gcaggctgtg atccatcgcg ctgggatggt cgtagttggt cttaacgcgt
    66181 tcttccatcg acagatggct ttgatctttg taatagcagt cttcgggaat tacgccgatg
    66241 tgttcatcac cgacttgctc acgtaattca cgataaaggg tactggcaat aagactcttg
    66301 ccggaagccg atgcgccagc gataccgata atgacgcact gatgagactg atcagtcata
    66361 tatttagcga cctgattaac ctggatgtta ggaagggggc gacgaagcgc caaacgcggc
    66421 aattataggg atttcatccg tctgatacca gtcgaatagc gttgccgcgc gctcagagtt
    66481 aattgttgac aaagaattcc cggtggcaaa ttacgttgat cagttttatg taaggtaaaa
    66541 aatgttatac gcagttgcgc aaattatccg cctttacgtc actttatgag caattcgcat
    66601 ataaaatgta aaacttttgt actagcataa acacagaaac gaatactggc gacctggtct
    66661 tgcggataaa gcggtaatga gcaaacaatc acagcatgta ttaattgccc tgccccaccc
    66721 gctgcttcac ctggtcagtt taggtttagt ctcgtttatc tttacccttt tctcgcttga
    66781 gctttcgcag tttggcaccc aactcgcccc actgtggttc ccgacgtcca tcatgatggt
    66841 ggcgttttat cgccatgccg ggcgcatgtg gccgggaatt gcgctgagct gttcgctggg
    66901 aaatatcgcc gcatccatcc tgcttttttc caccagctcg ctgaacatga cctggacgac
    66961 catcaatatt gttgaagccg tggtcggggc agtgctgcta cgtaaattgc tgccgtggta
    67021 taaccccttg caaaatctgg ctgactggct gcgtctggca ctcggcagcg ccattgttcc
    67081 gcctctgttg gggggtgttc tggttgtcct gctgacgccc ggagacgatc ctctcagggc
    67141 atttttgata tgggtactgt cagaatccat cggcgctctg gcactggtgc cgctgggatt
    67201 gttatttaaa ccacactatc tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct
    67261 gctcacgtta gccatcacac tgacgttaag ctggctttcg atgctgtatc tgccgtggcc
    67321 ttttactttc attattgtgc tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc
    67381 ctttttgatc ttccttacca cggtgatgat ggtgtcgctg atgatggccg cggatccctc
    67441 cctgcttgct acgccgcgta cgtacctgat gagccatatg ccgtggctac cgtttttgct
    67501 gatcctgctg cccgccaaca tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa
    67561 acacatttcc gaaagcgaaa cccgctttcg gaacgcgatg gaatattccg ctatcggtat
    67621 ggcgttagtg ggcaccgagg gacaatggct gcaaaccaac aaagcgctct gccagtttct
    67681 cgggtacagt caggaagagc tgcgcggact cacctttcag caactgacct ggccggagga
    67741 tctcaataaa gatctccaac aggttgaaaa gctgataagc ggtgaaataa acacctattc
    67801 aatggaaaaa cgctactaca accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc
    67861 actggtgcgc cacacggatg gcacgccgct ctattttatc gctcagattg aagacattaa
    67921 cgagctaaaa cgcaccgaac aggtcaatca gcaactgatg gagcgcatca cgctggctaa
    67981 cgaagcgggc gggattggca tctgggagag ggagttgaag ccgaatgttt ttagctggga
    68041 taagcggatg ttcgagctgt atgaaattcc tccacatatc aaaccgaact ggcaggtgtg
    68101 gtacgagtgc gtgctgccgg aagatcgcca gcatgccgaa aaagtgattc gtgattcgtt
    68161 gcaatcacgc tcgcccttta aactggaatt tcgcattacc gtaaaagacg gtattcgcca
    68221 tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gtctcctcgg
    68281 catcaatatg gatatgaccg aagtgaaaca gcttaacgag gcattgtttc aggaaaaaga
    68341 gcgcctgcac attacgcttg attccatcgg tgaagccgtg gtctgtattg atatggcgat
    68401 gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc aggaagaagc
    68461 gttaggtgtt ccgctcctga cggtgttgca tattactttt ggcgacaacg gaccattaat
    68521 ggagaacatt tacagtgccg acacctcacg ttccgcaatc gaacaagatg tggtgttgca
    68581 ctgtcggagc ggcggcagtt acgacgtgca ttacagtatt acgccgttaa gtactctgga
    68641 cggcagcaat attggttcgg ttctggtgat tcaggacgtc accgaatcac gcaaaatgct
    68701 gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca accgcgccag
    68761 ttttgagaaa caactgcgta tcctgctgca aacggtaaac agtacgcatc agcgacatgc
    68821 cctggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc
    68881 gggcgacgct ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc gctccagtga
    68941 cgtgctggcg cggctcggcg gtgatgaatt tggtctgctg ctgccagact gcaatgttga
    69001 aagcgctcgt tttatcgcta cacgtattat cagtgctgtg aatgactatc actttatatg
    69061 ggaaggccgg gtacatcggg taggtgccag cgccgggatt accttgatcg atgacaacaa
    69121 tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg
    69181 tggacggggc cgggtgacgg tttacgaacc acagcaagct gccgcacata gcgaacgggc
    69241 ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga tgatgatcgc
    69301 ccacggtgtc gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga tttcgcttaa
    69361 gctctggagt tgcgaaggcg aaattattga tgaacaaaca tttcgtcgta gcttcagcga
    69421 tccggcactt agccatgctc ttgaccgacg ggtattccac gaatttttcc agcaggccgc
    69481 caaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag
    69541 tagcgccacg ctggtgaatg atctgcttga gcagctggaa aatagccctc taccaccacg
    69601 gttattacat ctgattattc cggctgaagc gattttagat cacgcagaaa gcgtgcaaaa
    69661 actgcagctg gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt
    69721 caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa
    69781 cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg
    69841 actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc
    69901 tggcatcggc gtcgatctga tttatggtga tgtgattgcc gatgcccaac cgctggattt
    69961 gctggtgaat agcagttatt tcgcgattaa ctgagagagc gtttgcccat tgcctgatgc
    70021 gacgctaacg cgtcttatca tgcctacaaa tcgctcattc cccagaccgg ataaggcgct
    70081 cgcaccgcat ccggcgacca acgtcatgct tcgtctggtt gccagccttc cgtataccag
    70141 atatgcaaca gcgcataaga acgccagggc ttccagcgct cggcataacg gcggatttgt
    70201 gccggtgtca ttcccggaaa tcgccgttta atcagataat catccggcag aaaaacatct
    70261 ttcgcctgcc agccacgcaa agcaaaataa ttcgccgtcc agcgcccgat acccggaaaa
    70321 gtttgcagcg ttttcatcgc ctgctccaca tcgcccggaa ttgtcattgg taagctgccc
    70381 tccagcgccg catttgccag atgaatcagt gcctctgccc gtttcaacgg catgcctaac
    70441 gcttttaatg cctgcgggtc ggctgctgcc agccgctgag gcgtggggaa gcagatatac
    70501 tccggaaaat catccagccg ttcgccataa agctgtacca ctttggcagt caattttgcc
    70561 gccatcgcca cgctcaccag ttggcctaaa atcgcccgca caccctgctc aaaagcatca
    70621 atacagccgg gtaaacgcaa tccgggccgc cccgcgccta actttcccaa cgcaccgtta
    70681 acaatctgcg ggttacattg cagatcaagc aggcggctca ttttcgccag acactctgcg
    70741 gcaacaggtt ctaaacctgc acttaaattt atgtgcagag tatggcgggc tatatccgga
    70801 atagcagtca ctacgccgcg atattcgccc accgccagac tacgggcata ataattgtcc
    70861 gcgaccgttt ccacaccgct caccgcacgg gcggcgagaa atcccaacat ccacgaccag
    70921 tcatacggcg gctgccagtt cagggtatac atcgcatctc cttgttaatc cgctttcagc
    70981 ataaacgtta ttcagacgtt acgctttgct ttcatattcc ggttgtcgcg acagcaacat
    71041 ttcgctaaag tcacgcccct tcttcaccgg catggggatt aattcgtgtt tattggtttt
    71101 gattacggta cagcaaactg ttcagtggcg gtcatgcgtg acggtaaacc gcaattactg
    71161 aaaatggaaa acgacagcac gctgctgcct tcaatgcttt gcgcgccaac gcgtgaatcg
    71221 gtaagcgaat ggctgtaccg ccatcatgat gttccggcag acgacgatga aacgcaggcg
    71281 ctgttacgtc gggcgattcg ttataaccgc gaagaagata tcgatgttac ggcgaaaagc
    71341 gtgcagttcg gtctttcctc actggcacag tacattgatg atccagaaga agtgtggttt
    71401 gtgaaatcac caaaatcgtt cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg
    71461 tttgaggatc tggtctgcgc aatgatgttg cacattcgcc agcaggcgca ggcgcagctg
    71521 ccagaagcga ttactcaggc ggtgattggt cgcccgatca acttccaggg gctgggcggc
    71581 gatgaagcaa acgcccaggc gcaagagatt ctggaacgcg cggcgaagcg tgccggattc
    71641 agggacgtgg tattccagta cgagccgatc gcggctgggc tggattacga agccaccttg
    71701 caggaagaaa aacgggtgct ggtggtggat atcggcggtg gtacgactga ctgttcattg
    71761 ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg aagccagtct gctgggtcac
    71821 agtggttgtc gtattggcgg taacgatctg gatatcgccc tggcgtttaa aaacctgatg
    71881 ccactgctgg gtatgggtgg cgaaaccgaa aaaggcatcg ccctgccgat cctgccgtgg
    71941 tggaatgcgg ttgccatcaa cgacgtacct gcgcagagtg atttctacag tagtgccaac
    72001 ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac cggaaaaagt ggccctgtta
    72061 cagaaagtct ggcgtcagcg tttaagctat cgcctggtgc gtagcgcaga agagtgcaaa
    72121 attgctcttt caagcgtagc ggaaacccgc gcctcactgc cgtttatcag caatgaactg
    72181 gctacgctga ttagccagcg agggctggaa agcgccctca gccagccact ggcgcggatt
    72241 ctggaacagg tgcaactggc cctggataac gcccaggaaa aaccggacgt tatctacctg
    72301 accggcggta gcgcccgttc tccgcagatt aaaaaagcgc tggcagaaca gttgccgggc
    72361 attccgattg caggcggcga tgactttggc tccgtcaccg ccgggctggc acgctgggcg
    72421 gaagtggtgt ttcgttaatt gaaaaatggc tcagcagcgg cttaaatctg cctgcgcctg
    72481 agccagtttt tctgctgcct gacgtaacgg ttccatcaac ggcatggtcc gtgctggagc
    72541 atgaaggcgg aatcgctgca actctgtcgc gccgactgtc aacgccgatt ccagccgatg
    72601 tcgtttggcg gtcattttcg ccattaccgc ttgtctgtct gctggcgtta tcgcttcatt
    72661 cggcctgaac acaaaacggc gttcacagtt cgctttccag tcgataaccg cctgggtcag
    72721 atggtcgcca aaccctttca cttgcttaac gccacgacgg gtaacatctg cggctgtttc
    72781 aataccaaaa gaccgtaacg ccgctttacg cgcagggcca acgccgggaa tagaggcaac
    72841 atcaataaaa aatccctcca gaaacttctg cttctgccgt tcccttgcag tgtcatgaag
    72901 tgctgccaga gcgcgtttct cttcttccgg taacccgaga atttcgtcct tcattttttc
    72961 gagcatcgtc cgtttggcga taaaaccttc caggccgcct acctgctgga tctggcggac
    73021 taaatggtca tagtccattt tcgcacgatt aaatgcctca cggcgttgct gaacttctgc
    73081 tttgtacgct ttgcttgtca aactgccaat aatccagata gccgccagca caggaaccaa
    73141 aataatataa cgcggttctg cctgaaggcc gcaaagcagc agcgataatg ctgacagtgc
    73201 gatctcaagc agaatgatgt attcgcgccg taacaggcct aaaggaagcg gcctgccagt
    73261 cggttggaaa tgatcgggta atggcagttg caatgctggc ggtgctactg acgccatcac
    73321 catcgcccag acttttgcca gcacaaaatc cccgccggtg gtaatgacct cttcgccgag
    73381 atcaataaaa tagataacac cttgattatc cagcgcacac catgggcagt cagccaaatg
    73441 accgggataa acatgcattg ccgaaacggt acatttcttt aactgttggc gtagagaatc
    73501 cagtgctgat acccacgctt tagccgtcgg acgcccggtt gccacgccac tttccgtaaa
    73561 cgcctgctga aacatggctt caacatcact cggtaacatc gatagcggaa tcgatcgtgg
    73621 cggcggtttt aaaccacgtc gctgattatc tgacgcgtag gcataacgga aatgggtaat
    73681 atccgtctcc agcgcattac ccgcatcaga gataagcggc acaccagaat aaggatgccg
    73741 gccaccaaac aagacgtgaa aaatcagcaa cgcaaggcca aaattatcgt gattttctgt
    73801 gcgttcaaag ccgacaaatg acgacaaggt ttgtagctct ggcggcgtaa aatgcgacac
    73861 gccgacttcg cataaatgca gtgtgccatt ggcgttaatc tgaaaggagt cgctatcaat
    73921 caacaccact ttgctgtcgc gacctaccat aaaactgttc tggtttacgt cacccacgac
    73981 gtgcccgtgc tcgtgaaccg tagcaaaaga tgaagcaata ttgcgcgcaa catagagtag
    74041 aaaatcccac gcacaatgag ggtaacgttg acgacgatgt gccgggctat agatcatatg
    74101 aatcggttct ttaccagaaa cttttggcat cataaaaccg ataacttttc cgcctcgtcc
    74161 accgtgaagc gttgcctgcg gccaggcgac ataattcaac aattgcgcgt cagctgtcgc
    74221 agccataaag gcaagtttgt cctgttttaa ggcgggtggc ggcgtgtgat aaatcttggc
    74281 gacgctatcg acaaactcat tgatatcata aaccgcgcct tcaccgcctt tgcccagttc
    74341 acggcctggc gtaacgcact caccagtagc agtatataaa gtgggtttca tggtaaccac
    74401 agcgccagtg ccaacgtttt atcatcatcc gtacgctcgt ttaccgccgg gctggacaaa
    74461 aactgcttta acaattcagg taataaatcg agttgttcct gcgttgctga cgccagtcca
    74521 ttgaaaaacg gggtaaagaa aggcacatga ggagaattat ccagcatatt taacgccagc
    74581 cgctgaatac catccgtaaa tgctgcaact ttatgcgcac gcccagtgct ggtgaaagtt
    74641 tccagtctgg aaacggcatc ttcgtcggta atgaagtgcg tcatgttggc atattcaccc
    74701 accatcggcg tgagaggcaa ttgcagaccg tgacccaaat ctaccaccac gccgccatcg
    74761 ccaatctgca taatcaaggt gccgtcaggc gatgaaatca atcccagaaa cgtgcaggca
    74821 aaatcgcgaa ctgctaatgc tttggcttct gcctcggcaa aaagtcgttg acgaatagtc
    74881 agtaccatat ttgtggcgag gacatcatta agccccaatt caccgtcctg tactttttgc
    74941 accataaacg ccatcgcttc attgacggcg agcgtcgcgc cttctcctcc ctgcgaaacg
    75001 ctacctgcgc catcagcaag gaacatcacc agtaatggtt gctggtcgtt taaccagaca
    75061 acctgcatct ggcaggcatc ctgacaaggc aaatcagcac taatgtgcga tgttccaaca
    75121 gcggatgcat aaaccagacg ccagctcaca ctgacgtcca gccttttggc gcttccagca
    75181 caacttccgt tcccggcgtg gagcgggaaa ccgaacgcag cgaactggat aaccagctaa
    75241 acagttcacg gaattgtaat ccctgcagtg gcaaaggctg acgaacgctg atttgtgcca
    75301 gagtcttcat atccgccccc tgaacaccga tggaaaagaa ggcaaacttc ttatcttctt
    75361 ccccctgaaa cactttgttg gcggcagcct gccactcatc ggttggagcg ccatcggtga
    75421 tcaggaaaat ccacgggcga taatacgaaa taccatttgc gcggtactca cgtttcctct
    75481 cttcaaccat atccagggct ttggtaatgg cggcccccat aggcgtatcc ccctgggcaa
    75541 acaggatggg cgggaaaaag ttagcggcgc tggtaaaggg ttgttccaca tgcaccgggc
    75601 cgaacgtcac aatgccgagt tccactcttt ttaaagccag cggatcagca agcagttcat
    75661 cacgaaaggt aaccaatccg gcgttaagtt cattaatagg tctgccgctc atcgagccag
    75721 aaacatccag cagcaaaatg caggggcaac gtggttccgg gttactggca aaatcgctgg
    75781 tggcaaacgt gatttgttcg ctcataagaa atcctttctt agagaattat cgaggtccgg
    75841 tatcataccg attgcaataa tgaaataaga gcaaacgaag aaactttcag aattaggccc
    75901 gtcggcgcag aaaattttgc tattcgcctc gcaagaacgg agagccgtcg attgacacta
    75961 agggcggagt gacataattt caggagtgag ggttagggag aggtttcccc ctccccctgg
    76021 tgttcttagt aagcctggaa gctaatcact aagagtatca ccagtatgat gacgtgcttc
    76081 atcataaccc tttccttatt aaaagccctc ttctccggga gagggtttcc cgtttcagcg
    76141 tcccgctgaa atcgtcggct tacctccttt cgccctgaat gcagtctatc gctaacgcgt
    76201 gaacaaaata gtttcatctg tgattattgc gtaacgactc gcaaagaata tttgctggca
    76261 ggatcgcaga ctacaaagcc tgcggattga caatcttatc gtgaaggcat aatttcagga
    76321 gtgagggtag agcggggttt cccccgccct ggtagtctta gtaagcgggg aagcttatga
    76381 ctaagagcac cacgatgatg agtagcttca tcatgaccct ttccttatta aggccccttc
    76441 ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc
    76501 cctgtatgca gtctatcgct gacttgcgga taaaatagtt tccagtgtga ttattgcgta
    76561 acgtctcgca aagaatattt gctggcagga tcgcagactg caaagcctgc agattgacaa
    76621 tcttatcgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt
    76681 agccttagta agctggccag ctaatgacta agagcaccac gatgatgagt agcttcatca
    76741 tgaccctttc cttatttatg gccccttcct cgggaggggc tttcccgttt cagcgtcccg
    76801 ctgaaatcgg cggcttacct cctttcgcca tgcaagcagt ctatcgctaa cgcgtagata
    76861 aaatagtttc ctgtgttatt actggatgcg tgctcgcaaa tgtgcccgtc attcagacga
    76921 ttcccgacag tgtttcataa ttcctccatt tttctccctt attggctggc tacactagta
    76981 tcattccgcg aaacgtttca ggaagagaaa ctcttaacga tgaaaggcag ttataaatcc
    77041 cgttgggtaa tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc
    77101 aatgactccc agagtgcagc cccaggggcg acgaaacaag cgcagcaatc gccagcgggt
    77161 ggtcgccgtg gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa
    77221 caggcagttc cgcgttacct caccgggctt ggcaccatta tcgccgctaa tactgttacg
    77281 gtgcgcagcc gcgtggacgg tcaactgatg gcgttacatt tccaggaagg ccagcaggtc
    77341 aaagcaggcg atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa
    77401 acccagggcc aactggcaaa agataaagcc acgcttgcta acgcccgccg tgatctggcg
    77461 cgttatcagc aactggcaaa aaccaatctc gtctcccgtc aggagctgga tgcccaacag
    77521 gcgctggtca gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg
    77581 cagttgcaac tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag
    77641 caggttgatg ttggtaacca aatctccagt ggtgatacca ccggaattgt ggtgatcacc
    77701 cagacgcatc ctatcgattt agtctttacc ctgccggaaa gcgatatcgc tactgtagtg
    77761 caggcgcaaa aagccggaaa aacgctggtg gtagaagcct gggatcgcac caattcgaag
    77821 aaattaagtg aaggcacgct gttaagcctc gataaccaaa tcgatgccac aaccggtacg
    77881 attaaagtga aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt
    77941 aacgcgcgca tgttagtcga caccgaacaa aacgccgtag tgatcccaac agccgccctg
    78001 caaatgggca atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa
    78061 catctggtga cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttct
    78121 gcgggcgatc gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa
    78181 gtggtggaag cccagagcgc caccactccg gaagagaaag ccaccagccg cgaatacgcg
    78241 aaaaaaggag cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc
    78301 gcctgtttat tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg
    78361 ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca
    78421 ttcaggtggt cacgctctac ccaggtgcca gcccggatgt catgacctct gccgttaccg
    78481 cgccgctcga acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt
    78541 ccggcggtgc gtcagttatc actttgcagt tccagctaac attaccgctc gatgtcgccg
    78601 agcaggaagt gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta
    78661 acccgccggt ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca
    78721 cctcaaccgc catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga
    78781 aaatctcgca gatttccggc gtcggcctgg tgacgctttc cggcggtcag cgtccggctg
    78841 ttcgcgtcaa acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc
    78901 gcaccgccat taccggcgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc
    78961 gtgcggtcac gctttccgcg aacgaccaga tgcaatccgc cgaagagtat cgccagctaa
    79021 tcatcgccta ccagaacggc gcgccaattc gtctgggcga tgtcgcaact gtagagcaag
    79081 gtgcagaaaa cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg
    79141 ttcagcgcca gcccggtgct aacattatct ccaccgccga cagcattcgg cagatgctgc
    79201 cacagctcac tgagagtctg ccgaaatcgg tgaaggtgac agtgctttcc gatcgcacca
    79261 ccaatatccg cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg
    79321 tagtcatgat tatctacctg tttttgcgca atattccggc gaccatcatt cccggtgttg
    79381 ctgtaccgct gtcgttaatc ggcactttcg cggttatggt gtttctcgat ttttcaatca
    79441 ataacctgac actgatggcg ttaactatcg ccaccggatt cgtggtcgat gacgccatcg
    79501 tggtgatcga aaacatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc
    79561 tcaagggcgc aggtgaaatc ggctttacca ttatctcgct gaccttctca ctgattgcgg
    79621 tgttgatccc actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta
    79681 ttaccctggc ggtagcgatt ttgatctcag cggtggtgtc gctgaccctg acaccgatga
    79741 tgtgcgcgcg gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct
    79801 cggaaaaaat gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga
    79861 atcatccgtg gctgacctta agcgtggcac tcagcacgct gctgcttagc gtgctgctgt
    79921 gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa tggcattatt cagggcactt
    79981 tgcaggcacc gcaatccagc tcctttacca atatggccca gcgacaacgc caggtcgcgg
    80041 acgtgatttt gcaggatccg gcagtgcaaa gcctgacctc atttgttggc gttgatggca
    80101 ctaacccgtc gctgaatagt gcacgtttac aaatcaacct caaaccgttg gatgaacgtg
    80161 atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc ggtagataaa gtgccgggcg
    80221 tcgatctctt cctgcaacca acgcaggatc tgactattga tactcaggtc agccgcaccc
    80281 agtaccagtt taccttgcag gccacctcac tggatgcgct cagtacctgg gtgccacagt
    80341 tgatggaaaa actccagcaa ctgccacagc tttctgatgt ctccagcgac tggcaggacc
    80401 aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca
    80461 tggcggatgt cgataacgcc ctgtacaacg cgtttggtca gcgactgatt tccactattt
    80521 atactcaggc taaccagtat cgcgtagtgc tggagcacaa caccgaaatt accccgggcc
    80581 tcgcggcgct ggataccatt cgcctgacca gtagcgacgg cggcgtggtg ccgttaagct
    80641 caattgccaa agttgaacag cgttttgcgc cgctttccat caaccatctg gatcagttcc
    80701 cggtaacgac catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg
    80761 agattatgga caccgaaaag acgctgaatc tgccggtgga tatcaccacg caattccagg
    80821 gcagcaccct cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg
    80881 tggtggcgat gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca
    80941 ttctctcgac gctacccacc gcaggggtcg gcgcgctgct ggcattaatg attgccggta
    81001 gcgaactgga tgtgattgcg attatcggca ttattttgct gatcggtatt gtgaagaaga
    81061 acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc
    81121 gcgatgcaat ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg
    81181 cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc
    81241 gtccgttagg tatcggcatg gtcggcggtc tgattgtcag ccaggtgctg acgctgttca
    81301 ccacgccggt gatttatctg ctgttcgacc gcctggcatt gtggaccaaa agtcgctttg
    81361 ctcgccatga agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg
    81421 cgacgatttt actgtcggtc gccattaccc tgtgcggcat actgggcttc cgtatgctgc
    81481 cggtcgcccc gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg
    81541 gtgcgtcacc agaaacaatg gcgtcttccg ttgccacgcc gctggagcgc tcgcttgggc
    81601 gcattgccgg ggtcagcgaa atgacctcca gcagttcgct cggcagcaca cgtattattt
    81661 tacagtttga ttttgaccgg gacatcaacg gcgcagcgcg tgatgtgcag gcggcgatca
    81721 acgctgcaca aagtttgctg cccagtggga tgcccagccg tccgacctat cgcaaagcga
    81781 acccgtcgga tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggtg
    81841 aactgtacga tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggtgttg
    81901 gtgatgtcga tgtcggaggc agctcactgc ccgccgtacg cgtcgggctg aatccgcagg
    81961 cgctgtttaa tcagggcgtg tcgctggacg acgtacgcac cgccatcagc aatgccaacg
    82021 tgcgtaaacc gcagggcgcg ctggaagatg gcactcaccg ctggcagatc cagaccaatg
    82081 atgagctaaa aaccgccgct gaatatcagc cgttgattat tcactacaac aacggcggcg
    82141 cggttcgtct gggcgatgtg gcgacggtga ccgactcagt gcaggatgtg cgcaacgccg
    82201 ggatgaccaa cgccaaaccg gctattttac tgatgatccg caaactgccg gaagccaata
    82261 ttatccagac ggttgacagc atccgggcaa aattaccgga gttgcaggaa accattccgg
    82321 cggcgattga tctgcaaatt gcccaggatc gctcccccac cattcgcgcc tcgctggaag
    82381 aagtcgagca aacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttattcc
    82441 tgcgctcggg tcgcgccact attattcccg ccgtttcggt gccggtttcg ctgattggta
    82501 cgtttgcggc gatgtacctg tgcggattca gtctcaataa cctttcgtta atggcgctca
    82561 ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcacgtc
    82621 atctggaagc gggaatgaaa ccgttgcaag ccgcactgca aggtactcgc gaagtcggtt
    82681 ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg
    82741 gcggattgcc gggccgactg ttacgcgagt ttgccgtgac gctttctgtc gccattggta
    82801 tttcgttgct ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca
    82861 gcaagccgcg cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac
    82921 aaggctacgg caagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc
    82981 tgcttggcac cattgcgctg aatatctggc tgtatatctc gatcccgaaa accttcttcc
    83041 cggagcagga cactggcgtg ttgatgggcg ggattcaggc ggatcagagt atttcgtttc
    83101 aggcgatgcg cggtaagttg caggatttca tgaaaattat ccgtgacgat ccggcagtgg
    83161 ataatgtcac cggctttaca ggcggttcgc gagtgaacag cgggatgatg tttatcaccc
    83221 tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtaa
    83281 aactggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg
    83341 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    83401 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    83461 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgcgacacca
    83521 tggcacggct gggaatcgac gtgcaggccg ccaacagcct gttaaataac gccttcggtc
    83581 agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg
    83641 atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag
    83701 gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaacgct ccgctgtcgg
    83761 tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg accggaaaat
    83821 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga
    83881 cggtgcgcgg cagttttgcc ggaacagcgc aggtgttcca ggagacgatg aactcacagg
    83941 tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    84001 gttacgtaca cccactgacg attctctcca ccctgccctc ggcgggcgtt ggtgcgctgc
    84061 tggcgctgga gctgttcaat gccccgttca gcctaatagc cctgataggg atcatgctat
    84121 taatcggcat cgtaaagaaa aacgccatta tgatggtcga ttttgcgctc gaagcccaac
    84181 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    84241 cgattatgat gactaccctg gcggcgctgt ttggcgcgct gccgctggtg ttgtcgggcg
    84301 gtgacggctc ggagctgcgg cacctcctgg ggatcaccat tgtcggtgga ctggtaatga
    84361 gccagcttct gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    84421 tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagaa cttcccgaca
    84481 gcacccgctg gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    84541 ccatcgttaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    84601 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    84661 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    84721 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    84781 tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg atgaaaatcg
    84841 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    84901 cgctgctcgg tccggcgctc ggcggtctgc tggtggagta cgcgtcgtgg cactggatct
    84961 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgctg ttaatgccga
    85021 actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg gcggttggca
    85081 tggctgtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga
    85141 ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa
    85201 ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc
    85261 tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt
    85321 tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg atcccgatgg
    85381 tacttggcag catgggaatg aagcgaattg tggttcaggt ggtgaatcgc tttggttatc
    85441 gtcgggtact ggtagcgacc acgctgggtc tgtcgctgat caccctgttg tttatgacta
    85501 ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa gggatggtca
    85561 actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    85621 cgagcagcgg caacagtctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    85681 ccatcgccgg gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca
    85741 ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg
    85801 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    85861 aaaggagcgc gcaatgaagt tctggcgacc tggtattacc ggcaaactgt ttctggcgat
    85921 tttcgccacc tgcattgttt tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    85981 gcgtggcttt attgattaca tcaagcatgg taatgaacag cgattacaac tgttaagtga
    86041 tgcgcttggc gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg
    86101 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc
    86161 gggtatgcca ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt
    86221 gctggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa
    86281 cggtgcggaa gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga
    86341 tatcaatttc gataaacaac agcggcaaac cagctggttg attgtcgccc tggcaacgtt
    86401 actcgcggca cttgccactt ttctgctggc gcgcggttta ctggcaccgg taaaacgact
    86461 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    86521 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag cttgccagca cactggagaa
    86581 aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt
    86641 agcggtgctg cgcggtgaac tggaagctat tcaggatggc gtgcgcaaat tcacgccgga
    86701 gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg acgatctcca
    86761 tcagttgtcg atgtctgatg aaggcgccct cgcctatcaa aaagcaccgg tagatttgat
    86821 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc gtggcctgaa
    86881 actgcaattt tccctgccag acagtattac cgtatttggc gatcgcgacc gtttaatgca
    86941 gttattcaat aatttacttg aaaacagcct gcgctacact gacagcggcg gcagcctgaa
    87001 aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    87061 tgtcagtgac gagcagctac aaaaattatt tgaacgtttt tatcgcaccg aaggttcccg
    87121 caaccgcgcc agcggtggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    87181 acataatggc cgcattattg ctgcccattc gccttttggc ggggtaagca ttacagtaga
    87241 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    87301 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat
    87361 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actgccgtat
    87421 gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg caccgatggc
    87481 ttgacgctgt gccgggaaat tcgtcgtttt tctgacattc ctatcgtgat ggtgacggca
    87541 aaaatcgaag agatcgaccg cctgctcgga ctggagattg gcgcagatga ctacatctgc
    87601 aagccgtaca gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa
    87661 ccgcagcgcg aattgcagca acaggatgcc gaaagcccgt tgattatcga cgaaggtcgt
    87721 tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgttg
    87781 aaaacgctct ctcatgaacc aggaaaagtg ttctcccgcg agcaattgct caatcatctt
    87841 tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa aaacctgcgc
    87901 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgttggt
    87961 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatga ctttgttagc
    88021 cggagatggc gttcagacca gatccggcaa cattatccca cgcatggtca gcaaactg
//
LOCUS       DAJTVJ010000002        79579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000002 DAJTVJ010000000
VERSION     DAJTVJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.002"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="OO448_000089"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="OO448_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="OO448_000090"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="OO448_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6335424.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="OO448_000091"
     CDS             2354..2758
                     /locus_tag="OO448_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6335425.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="OO448_000092"
     CDS             2811..2921
                     /locus_tag="OO448_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335426.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="OO448_000093"
     CDS             3157..3201
                     /locus_tag="OO448_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335427.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="OO448_000094"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="OO448_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6335428.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO448_000095"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO448_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6335429.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="OO448_000096"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="OO448_000096"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6335430.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="OO448_000097"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="OO448_000097"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6335431.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="OO448_000098"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="OO448_000098"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6335432.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="OO448_000099"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="OO448_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6335433.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="OO448_000100"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="OO448_000100"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6335434.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="OO448_000101"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="OO448_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6335435.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="OO448_000102"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="OO448_000102"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6335436.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="OO448_000103"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="OO448_000103"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6335437.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO448_000104"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO448_000104"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6335438.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="OO448_000105"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="OO448_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6335439.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="OO448_000106"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="OO448_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6335440.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="OO448_000107"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="OO448_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6335441.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="OO448_000108"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="OO448_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6335442.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO448_000109"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO448_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6335443.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO448_000110"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO448_000110"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6335444.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO448_000111"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO448_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6335445.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO448_000112"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO448_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6335446.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO448_000113"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO448_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6335447.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="OO448_000114"
     CDS             23801..24874
                     /locus_tag="OO448_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6335448.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25002..28448
                     /gene="mfd"
                     /locus_tag="OO448_000115"
     CDS             25002..28448
                     /gene="mfd"
                     /locus_tag="OO448_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6335449.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28592..29548
                     /gene="ldtC"
                     /locus_tag="OO448_000116"
                     /pseudo
     CDS             28592..29548
                     /gene="ldtC"
                     /locus_tag="OO448_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO448_000117"
     CDS             complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO448_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6335450.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30129..30761
                     /gene="comR"
                     /locus_tag="OO448_000118"
     CDS             30129..30761
                     /gene="comR"
                     /locus_tag="OO448_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6335451.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30823..31362)
                     /locus_tag="OO448_000119"
     CDS             complement(30823..31362)
                     /locus_tag="OO448_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6335452.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO448_000120"
     CDS             complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO448_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6335453.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33301..33843)
                     /locus_tag="OO448_000121"
     CDS             complement(33301..33843)
                     /locus_tag="OO448_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6335454.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="OO448_000122"
     CDS             complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="OO448_000122"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6335455.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="OO448_000123"
     CDS             complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="OO448_000123"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6335456.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="OO448_000124"
     CDS             complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="OO448_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6335457.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36362..36739)
                     /locus_tag="OO448_000125"
     CDS             complement(36362..36739)
                     /locus_tag="OO448_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6335458.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="OO448_000126"
     CDS             complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="OO448_000126"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6335459.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37435..39624
                     /gene="fhuE"
                     /locus_tag="OO448_000127"
                     /pseudo
     CDS             37435..39624
                     /gene="fhuE"
                     /locus_tag="OO448_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="OO448_000128"
     CDS             complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="OO448_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6335460.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41412..42209)
                     /locus_tag="OO448_000129"
     CDS             complement(41412..42209)
                     /locus_tag="OO448_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6335461.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42220..43224)
                     /gene="holB"
                     /locus_tag="OO448_000130"
     CDS             complement(42220..43224)
                     /gene="holB"
                     /locus_tag="OO448_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6335462.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43221..43862)
                     /locus_tag="OO448_000131"
     CDS             complement(43221..43862)
                     /locus_tag="OO448_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6335463.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="OO448_000132"
     CDS             complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="OO448_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6335464.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="OO448_000133"
     CDS             complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="OO448_000133"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6335465.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="OO448_000134"
     CDS             complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="OO448_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6335466.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="OO448_000135"
     CDS             complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="OO448_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6335467.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="OO448_000136"
     CDS             complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="OO448_000136"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6335468.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="OO448_000137"
     CDS             complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="OO448_000137"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6335469.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="OO448_000138"
     CDS             complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="OO448_000138"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6335470.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="OO448_000139"
     CDS             complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="OO448_000139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6335471.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="OO448_000140"
     CDS             complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="OO448_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6335472.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="OO448_000141"
     CDS             complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="OO448_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6335473.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52391..52975
                     /gene="yceF"
                     /locus_tag="OO448_000142"
     CDS             52391..52975
                     /gene="yceF"
                     /locus_tag="OO448_000142"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6335474.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="OO448_000143"
     CDS             complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="OO448_000143"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6335475.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54619..57804
                     /gene="rne"
                     /locus_tag="OO448_000144"
     CDS             54619..57804
                     /gene="rne"
                     /locus_tag="OO448_000144"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6335476.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="OO448_000145"
     CDS             complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="OO448_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6335477.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="OO448_000146"
     CDS             complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="OO448_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR6335478.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="OO448_000147"
     CDS             complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="OO448_000147"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6335479.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="OO448_000148"
     CDS             complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="OO448_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6335480.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="OO448_000149"
     CDS             complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="OO448_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6335481.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="OO448_000150"
                     /pseudo
     CDS             complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="OO448_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="OO448_000151"
     CDS             complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="OO448_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR6335482.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="OO448_000152"
     CDS             complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="OO448_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6335483.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="OO448_000153"
     CDS             complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="OO448_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6335484.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="OO448_000154"
     CDS             complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="OO448_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR6335485.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="OO448_000155"
     CDS             complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="OO448_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6335486.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68109..68768
                     /gene="flgA"
                     /locus_tag="OO448_000156"
     CDS             68109..68768
                     /gene="flgA"
                     /locus_tag="OO448_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6335487.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVQDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68844..69137
                     /gene="flgM"
                     /locus_tag="OO448_000157"
     CDS             68844..69137
                     /gene="flgM"
                     /locus_tag="OO448_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6335488.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            69142..69558
                     /gene="flgN"
                     /locus_tag="OO448_000158"
     CDS             69142..69558
                     /gene="flgN"
                     /locus_tag="OO448_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6335489.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="OO448_000159"
     CDS             complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="OO448_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6335490.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71243..72166)
                     /locus_tag="OO448_000160"
     CDS             complement(71243..72166)
                     /locus_tag="OO448_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6335491.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72168..72815)
                     /locus_tag="OO448_000161"
     CDS             complement(72168..72815)
                     /locus_tag="OO448_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6335492.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="OO448_000162"
     CDS             complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="OO448_000162"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6335493.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73646..74854
                     /gene="mdtH"
                     /locus_tag="OO448_000163"
     CDS             73646..74854
                     /gene="mdtH"
                     /locus_tag="OO448_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6335494.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74918..75565
                     /gene="grxB"
                     /locus_tag="OO448_000164"
     CDS             74918..75565
                     /gene="grxB"
                     /locus_tag="OO448_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6335495.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75699..76259
                     /locus_tag="OO448_000165"
     CDS             75699..76259
                     /locus_tag="OO448_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6335496.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76365..77411
                     /gene="pyrC"
                     /locus_tag="OO448_000166"
     CDS             76365..77411
                     /gene="pyrC"
                     /locus_tag="OO448_000166"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6335497.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77485..77730
                     /gene="dinI"
                     /locus_tag="OO448_000167"
     CDS             77485..77730
                     /gene="dinI"
                     /locus_tag="OO448_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6335498.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78020..78274
                     /gene="bssS"
                     /locus_tag="OO448_000168"
     CDS             78020..78274
                     /gene="bssS"
                     /locus_tag="OO448_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6335499.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78389..79507
                     /gene="solA"
                     /locus_tag="OO448_000169"
     CDS             78389..79507
                     /gene="solA"
                     /locus_tag="OO448_000169"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6335500.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19598 a        20912 c        19710 g        19359 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aaagatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgataccgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccaac gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttgggaatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaattgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gtaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    25021 atacactgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    25081 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    25141 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    25201 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    25261 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga
    25321 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    25381 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    25441 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    25501 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    25561 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    25621 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    25681 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    25741 aagtgagtaa aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg
    25801 aaccactgcc gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    25861 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    25921 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    25981 tcttctcaga gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa
    26041 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac
    26101 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    26161 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    26221 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    26281 ttggcgctgc cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa
    26341 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    26401 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    26461 agcacggtgt cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg
    26521 agtatttgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    26581 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    26641 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    26701 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    26761 gtgagcagta tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    26821 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    26881 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    26941 ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg
    27001 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    27061 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    27121 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    27181 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    27241 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa
    27301 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    27361 aaacctttgt ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt
    27421 tgcgcggggg gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg
    27481 aacggctggc agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg
    27541 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    27601 gtacaaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    27661 gcgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc
    27721 atcatcaggc atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac
    27781 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    27841 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    27901 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    27961 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    28021 ggatgccgtc gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct
    28081 ataaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    28141 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    28201 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    28261 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    28321 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    28381 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    28441 tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    28501 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    28561 acaataacct tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg
    28621 gctaacgttt tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc
    28681 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    28741 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    28801 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    28861 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    28921 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    28981 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    29041 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    29101 gcctacggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    29161 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    29221 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    29281 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    29341 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    29401 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    29461 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    29521 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    29581 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    29641 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    29701 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    29761 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    29821 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    29881 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    29941 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    30001 attaatttaa aaatctttaa atttcagtta aatagaaagc aattattttt atgggatctg
    30061 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggttca
    30121 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    30181 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    30241 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
    30301 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    30361 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    30421 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    30481 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga
    30541 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    30601 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    30661 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    30721 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc
    30781 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    30841 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    30901 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    30961 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
    31021 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc
    31081 tttaccacga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
    31141 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg
    31201 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
    31261 gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc
    31321 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt
    31381 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
    31441 cgcgggaatg gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga
    31501 cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt
    31561 ttatgccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc
    31621 ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct
    31681 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
    31741 agccgcgcgt caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca
    31801 gcgaaccatg atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa
    31861 tatttttcat tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc
    31921 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg
    31981 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
    32041 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
    32101 tatattcacc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
    32161 tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg
    32221 gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg
    32281 tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc attgtacaat tcagcggaga
    32341 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca
    32401 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
    32461 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg
    32521 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt
    32581 cacctttctc atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
    32641 tgacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
    32701 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat
    32761 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
    32821 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
    32881 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa
    32941 acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata
    33001 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
    33061 ataggcgact ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag
    33121 tccggaaggg tgatataggg tgacgcgtat atcgagggcg tttggtcgtc ctgataagat
    33181 gcgcagcatc gcatcaggca ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc
    33241 gacttacaga attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat
    33301 ttacccgagg gttttgaacg ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt
    33361 gtgcgtctgc tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg
    33421 ctggctgtta agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt
    33481 ctcgcggaag ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat
    33541 cttgccttcc atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc
    33601 gcaaagaaaa ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg
    33661 acgctcgtcg acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc
    33721 atgtttcgga tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa
    33781 ttgtaagact ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat
    33841 catcgcttcc tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat
    33901 gtaactgatt cagacgggtg ctgatcgcct tccagcgagc cgagtccatc agttcctgtc
    33961 gcgaaaatga acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat
    34021 tatctaacac gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacctg
    34081 cgtccagtga tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg
    34141 ataaatcgtc agagaaaatt acaccgtcaa aacacagttc ctgacgcaaa acggttttca
    34201 gccagtagga agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag
    34261 gcataatggc gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag
    34321 cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta
    34381 ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat
    34441 caataaaccg gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc
    34501 caatcgccgc gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca
    34561 tagcgatcat ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt
    34621 ccattcccaa cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa
    34681 aacgctgcac gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg
    34741 cgcggatctg gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga
    34801 gaatcagccc tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt
    34861 aaccttcgac atccaacatt actggaccca cactgctctc cttattgttt tattaatagc
    34921 tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccgatttg tcgccagcgg
    34981 tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc
    35041 cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc
    35101 tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca
    35161 tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca
    35221 tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt
    35281 aacattcgca gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc
    35341 agtaagggca acagcgttat tcgccagcca aaacgtggtt gttgatgaag ataatacagc
    35401 aagcctgcca gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg
    35461 actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt
    35521 gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga
    35581 cgcacaacaa aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta
    35641 ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg
    35701 atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac
    35761 cagcatcagc tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag
    35821 cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact
    35881 gtcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag
    35941 ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt
    36001 cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac
    36061 cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg
    36121 gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg
    36181 cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc
    36241 cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag
    36301 aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc
    36361 cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
    36421 gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct
    36481 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
    36541 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
    36601 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
    36661 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc
    36721 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
    36781 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt
    36841 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
    36901 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat
    36961 gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
    37021 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
    37081 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
    37141 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct
    37201 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc
    37261 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
    37321 atgcgcatat ttctcatttg catttacaaa caaaattatt cgcacactaa atataaccgc
    37381 tcatcaccct cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt
    37441 tcaacacaat ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc
    37501 ggttgcatag cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa
    37561 acggtgattg ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta
    37621 acgtctacct ctgcgggtac caaaatgcag atgactcaac ttgatattcc tcagtcggtc
    37681 actattgtta gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg
    37741 gaaaacacgc tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc
    37801 cgtggattcc agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc
    37861 tggaatctgg gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt
    37921 ggcgcgacag gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga
    37981 aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac
    38041 aaagaacggt atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg
    38101 cgaattgtcg gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag
    38161 accttcttct cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt
    38221 tacgaatatc agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat
    38281 actgatggca gcagcaacag ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac
    38341 aacgataaag agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctag
    38401 caagcgacac tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc
    38461 gatgcctatg taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct
    38521 ggctttgatt atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg
    38581 gatttgttcg ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt
    38641 ggcagttaca gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat
    38701 gaaattggca gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag
    38761 agcctggcac aggacgatac cacacatatg aaatcgttat atgctgctac tcgagtcacc
    38821 cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg
    38881 ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc
    38941 aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt
    39001 gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa
    39061 tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat
    39121 aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa
    39181 gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac
    39241 aactggcagc tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc
    39301 gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc
    39361 atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc
    39421 gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc
    39481 acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac
    39541 aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc
    39601 attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggcggcct
    39661 tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc
    39721 aggttatcgg atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc
    39781 gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg
    39841 cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca
    39901 accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca
    39961 cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca
    40021 atcgcataac cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga
    40081 acgataaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata
    40141 cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg
    40201 aacatgaagg agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg
    40261 gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca
    40321 attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca
    40381 gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg
    40441 gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggc
    40501 accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca
    40561 gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca
    40621 acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag
    40681 aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc
    40741 gcaccggaga taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct
    40801 tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca
    40861 taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca
    40921 ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca
    40981 tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct
    41041 gcgataggca gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca
    41101 aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc
    41161 acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat
    41221 tacacagagt aaaataattc agtgccgatt tgtttgacgg ctatcacatt tcaacttcag
    41281 atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta
    41341 ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta
    41401 aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg
    41461 aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg
    41521 tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc
    41581 gcaaggtaag gtgagtcagt ttccaccagt aaccgatcca gggggacata acgcgcagca
    41641 tctcgaagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg
    41701 agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg
    41761 caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta
    41821 tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct
    41881 tgctgacgaa ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct
    41941 acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg
    42001 ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc
    42061 atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg
    42121 gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg
    42181 agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag
    42241 cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc
    42301 gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat
    42361 agcctgcagg cgcgagggag aaagatggtt tgccagttcg acgacaaggc ccggcacatc
    42421 aacattggtc acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc
    42481 cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca
    42541 gtcgcccgat ggcacgctat atgccaatgc ctgacacaat gtttcacgag cctgccagtt
    42601 atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc
    42661 aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc
    42721 cggcggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc
    42781 aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag
    42841 caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc
    42901 accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtactg catcaatgcc
    42961 cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg
    43021 catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag
    43081 taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat
    43141 gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg
    43201 tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg
    43261 cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta
    43321 tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct
    43381 tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta
    43441 acatcgagat agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt
    43501 gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg
    43561 tggcgatcgc caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc
    43621 agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg
    43681 cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca
    43741 ggttcccgag tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca
    43801 ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc
    43861 atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt
    43921 ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc
    43981 cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa
    44041 accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt
    44101 gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc
    44161 aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt
    44221 ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc
    44281 gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt
    44341 gagtaacgcg acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc
    44401 ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc
    44461 actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg
    44521 catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca gcaatttcag
    44581 catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag
    44641 atccggttcg atacgcagca accattgaaa aacccgtggg cgattgatga tcttatcggc
    44701 ataaagctgt tcaccaagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc
    44761 ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc
    44821 ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa
    44881 ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca
    44941 tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac catctcatct
    45001 gcctgcaacg cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc
    45061 agcaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagacgcggc
    45121 gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac
    45181 ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc
    45241 aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc
    45301 cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga
    45361 taaaccgtaa cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc
    45421 ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc
    45481 gttttcatct cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa
    45541 gcatcctgta gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg
    45601 gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc
    45661 tgcttatgac cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca
    45721 gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc
    45781 ttttatatgg gtgggaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc
    45841 caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat
    45901 gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa
    45961 cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta
    46021 acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga
    46081 agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg
    46141 cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg
    46201 ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca
    46261 taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact
    46321 cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc
    46381 acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa
    46441 aaccaccaac acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag
    46501 catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg
    46561 cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca
    46621 ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca
    46681 tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg
    46741 caccaatgcg ggttgcgttc tcttccgtta tatcaaggcc agaatcctgc atggcctgaa
    46801 cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga
    46861 taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag
    46921 tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag
    46981 actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct
    47041 tagacacgtt tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac
    47101 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    47161 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    47221 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt
    47281 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    47341 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    47401 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    47461 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    47521 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    47581 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    47641 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    47701 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    47761 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    47821 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    47881 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    47941 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa
    48001 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    48061 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    48121 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc
    48181 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    48241 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    48301 ttccttcaaa attcatgatt ttcctctttt aaagctcaag cgccgctgcc atcgctgaag
    48361 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    48421 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    48481 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    48541 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    48601 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    48661 acggtacgct cactggtaac ggcagagcac gttttgcgcc cgcagcttta caggcagcgc
    48721 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    48781 ttaccggaga aacgacctga cctgctgcag cttcttcaca cgctttcgca atagacgcat
    48841 catccagacc gatgattgcc gccatagcgc ccgtaccttc cggtacggct tcttgcatga
    48901 gcttgccgcg catctcaacc agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa
    48961 ccagcgcgga atattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    49021 gctgctgcca tacgcggtac agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc
    49081 aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca
    49141 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    49201 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    49261 tttaatcctt atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg
    49321 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    49381 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    49441 ccatagacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    49501 gaaccagcca gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga
    49561 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    49621 ttgaattctc tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac
    49681 cgtcggcatg cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag
    49741 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg
    49801 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    49861 cggctacgct taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc
    49921 aacctttaat gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    49981 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    50041 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    50101 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    50161 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    50221 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    50281 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    50341 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    50401 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    50461 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    50521 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    50581 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    50641 atcgtcttta atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    50701 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    50761 accgctgagg catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc
    50821 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    50881 gcaaattgca ccaacattgt actgtcacaa tcgacgttgg cccctaagtc aaggaccacc
    50941 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    51001 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    51061 caggcttgcg ctcgaccttc tttcaccagc tccagggcca cgcgcattga actcccacga
    51121 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    51181 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    51241 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    51301 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    51361 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    51421 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    51481 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    51541 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    51601 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    51661 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    51721 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    51781 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    51841 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    51901 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    51961 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    52021 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    52081 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    52141 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    52201 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    52261 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    52321 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    52381 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    52441 ggaaaaactg caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag
    52501 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc
    52561 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    52621 aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg
    52681 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    52741 aacagaagtg gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa
    52801 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    52861 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    52921 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    52981 tgcgctggat gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa
    53041 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    53101 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    53161 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    53221 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    53281 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    53341 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    53401 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    53461 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    53521 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    53581 ttttcgctat cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    53641 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    53701 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    53761 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    53821 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    53881 gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg taaatcatac
    53941 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt
    54001 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    54061 attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta
    54121 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    54181 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    54241 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    54301 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    54361 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    54421 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    54481 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    54541 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    54601 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    54661 cgcgttgccc ttgtagatgg gcagcgtctt tatgacctgg atatcgaaag tccagggcac
    54721 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    54781 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    54841 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    54901 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    54961 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    55021 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    55081 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    55141 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    55201 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    55261 gttcgcgcat tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg
    55321 aaagtgctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    55381 aaaatcaaac tgtataccgg cgagatcccg ctgttcagcc actaccagat tgagtcgcag
    55441 atcgagtccg ccttccagcg tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac
    55501 agcaccgaag cgttaacggc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    55561 atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg
    55621 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc
    55681 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    55741 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    55801 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    55861 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    55921 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    55981 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    56041 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    56101 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    56161 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    56221 ccggatgtgc cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    56281 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    56341 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    56401 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    56461 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    56521 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt
    56581 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg
    56641 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    56701 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    56761 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    56821 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    56881 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    56941 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    57001 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    57061 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    57121 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    57181 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactggggtc
    57241 cctgtcgccg ccgctgtcga acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa
    57301 gttgtagaag cccccgttca gattgccgaa ccgcaaccgg aagtggttga aacgacgcat
    57361 cctgaagtga ttgccgccgc agtaactgaa cagccgcagg tgattaccga gtctgatgtt
    57421 gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg
    57481 gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt
    57541 gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg
    57601 gttgaacctg aggtcaccgt tgagcataac cacgctacgg tgccgatgac gcgcgctccg
    57661 gcaccggaat atgttccgga tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc
    57721 ttcgaaggta aaggtgccgc aggtggtcat acggcaacac atcatgcctc tgccgctcct
    57781 gcgcgtccgc aacctgttga gtaataatta gctcaagtaa tcaagccctg gtaactgcca
    57841 gggctttttt atttcatctt tgaatcaaca tattaaaatt catcgaatgg catccttgct
    57901 aaccaacaat gcaaaatagg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac
    57961 aaaacatacc cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc
    58021 gacaatccct gcatatcggt aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg
    58081 atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt
    58141 tgccctaaag cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg
    58201 cctaattccg cgcgaacggt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt
    58261 ttatctaacg ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc
    58321 agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct
    58381 ggttccgcta ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc
    58441 accatcgaac gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc gacataatca
    58501 ccgttagcct cactaaacgg cgcagtctct gttttataac cggcaaaaat gtagcgccca
    58561 ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta
    58621 gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc
    58681 tgagcattct ggatagcagt ggtgacctgg ctaagtacac tctcttccag tgacactttt
    58741 tgagtggcga aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga
    58801 acaaccgctt gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc
    58861 gacatctgtt cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt
    58921 tgctggtaca tcatctgggt actgaaacgc attttatctc cccttagcga atgttaatca
    58981 gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct
    59041 gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct
    59101 gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg
    59161 ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc
    59221 ccaccgtttt actattgctt tgcagatcca gcagggcctg accgttgcgg ttatcgctat
    59281 cacccgcatc tttttcgctc gccatcgcta ttttcgcttc gtcggtgatt aatacatcca
    59341 tgttgacgat ggcgtcactt actggtttca gcgtgaagct gtcgttaacg gcaggcgttc
    59401 ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg
    59461 taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg aacgagattt
    59521 tgtaatctgt cgccagtacc acggaggcat cagttaccgt ggcaccgatc gcaacgtcac
    59581 ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag
    59641 catcgccgtt agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
    59701 gcgccagttg tccaagcgta ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa
    59761 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc
    59821 catcaacata agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt
    59881 gccgcgccgt acttccctga accagtgagt aaccattggc caccgtgatg ttataagtgc
    59941 cgccatcctg aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat
    60001 cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga
    60061 tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca acgctggcac
    60121 ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact
    60181 gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt
    60241 tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg
    60301 tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc
    60361 tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca
    60421 caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct
    60481 gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac
    60541 ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc
    60601 tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag cgacccgccg
    60661 gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc
    60721 gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt gaggatcggt
    60781 agcatacccc gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac
    60841 ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg cttccagata
    60901 cgagctgtag acccgaaatt tcgcttttac tttcttcgct tcgccatttt catattcagt
    60961 cgtggtgatt tcagtaactg gacctttcca gttgccagag gctttgacgc caaacaggtt
    61021 atagctcggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag actccagcgc
    61081 cgcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca gttgggcggg
    61141 cagcgagagt tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg
    61201 tggcacggcc ttttgcacca gctgcgaaag cgcctgattt tgataacgca ccacagtttc
    61261 gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt
    61321 catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca tctgttgggc
    61381 aatctgctgg tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc
    61441 ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg
    61501 acgggccacc ggacggatat ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt
    61561 gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg
    61621 atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcagtat ggacatcaga
    61681 tccatcggcg tagcgcccag cgcattgagc gcgcgcacca cgttattgag gctggcgctg
    61741 gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta
    61801 accacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc ctgacgattg
    61861 actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc
    61921 atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta
    61981 acctgcatat tctggatatc tgcaaggaaa cggacctggg aactgttgcc acttggtacg
    62041 cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg
    62101 atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg caaattaagg
    62161 gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc
    62221 cgtccaccgt tcagttggtt cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg
    62281 ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc cttaagcggc
    62341 gtcatcaaca acgtacctcc acgcaagctt ttggcatttc ccatggaaga aaccaccaca
    62401 tcgatggttt gcccctgacg tccaaacgga ggaagtgact ctgtcaccat taccgcagcg
    62461 acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg
    62521 ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccggt gccatccagc
    62581 cccaccacca gaccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga
    62641 tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa tgcagagaga
    62701 aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa cgctgcaacc
    62761 agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaatg cgcgcatccg
    62821 ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag
    62881 agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc accacatgca
    62941 ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc
    63001 tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac
    63061 gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt
    63121 taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca
    63181 cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc
    63241 catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg
    63301 gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc caggcgcagc
    63361 cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca
    63421 tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttaa gccttagagt
    63481 tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa
    63541 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    63601 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca
    63661 ccagaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagccc ggtgtcattc
    63721 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt
    63781 acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc
    63841 gctggctgca cctgaaaacc accagctgcc cgttctgatc cacctggaaa gagccgtcac
    63901 gggtataggc tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg
    63961 cgacatcttt gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg
    64021 cgaccgggcg tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag
    64081 actgtgcccc cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac
    64141 gcttaaaacc gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt
    64201 gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag
    64261 tggattagtg acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg
    64321 cctacaagtg ctcgcaaaat cgataaattg cgattgcccc catagacccg ataaacttgc
    64381 gcatcgggca acttatactt gtcatgtatc ctttcaatta actcatcgac agcagttggt
    64441 tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc
    64501 gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa
    64561 cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg
    64621 tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt
    64681 tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg
    64741 ccgagattgt gccatcggca gcaatagtga tttccgctcc ttccggcaca gcaattggcc
    64801 ccgcctcgcc tatcacctga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa
    64861 tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccagccat
    64921 cctgctgcaa cgcgacgtcc agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat
    64981 ctgcgcccgg cgttgacgcc gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg
    65041 gcaccgcgcg taaagcattc aactgcgcgc gaaaaccggg cgttgaggca ttggccagat
    65101 tactggcggt taccgcctgt tgattcagtg tctggctggc tgctcccatc gcggtataaa
    65161 ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag cgtgttgagg
    65221 atctggtcct gagttttgat ggtctgggcg ttagactgat agttacgctg ggcaacgatc
    65281 atattgacca gttctttact gagatcgaca ttggacgctt ccaacgcacc gttggtcagg
    65341 gtgccaaagt tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca
    65401 gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca
    65461 atctgcccca gcagttgtgt ttgttcgttg gaatagttgc cgacaaccgt accgtcatca
    65521 ttgatttgat aactcaccag atcgcccggt ttgtagccat tctgggtggt tgccacaata
    65581 ttgttagcgc cggtattttg ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga
    65641 tctgcgccgt taattgcgcc agtggtaata ttctctgttg gatttgaggt cagaacgcca
    65701 ttggcattaa acaccagctt cattgcaggc tcggctgtac ctgttggatc actgctatcc
    65761 tgggtgtaga cctgccagtt attatccccg gtcttcacaa agtagacgct catgtcatga
    65821 gcattaccct gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg
    65881 ctggcgctga atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc
    65941 gacgccgtgg tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga
    66001 ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa
    66061 ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag
    66121 aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc
    66181 agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact
    66241 tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat
    66301 ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg
    66361 gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag
    66421 gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc
    66481 gtattaccgc tgttgccgcg gagcacaccc tgcaccagag caaactgcag cggctgggca
    66541 accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca
    66601 gtgctgccat cagtcaacgt accgtcccag gtgaaactgt ggactccggc ggtcagttca
    66661 ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg
    66721 tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca
    66781 ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc gatcaggtta
    66841 ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg
    66901 agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt
    66961 tccattggat tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt cagaaaactg
    67021 ctttgtaagt ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg
    67081 gtatttgtcg gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga
    67141 gcgtaagggt tttcagcatc atgcttttca ccgtgttgag cacttcaaca ttggcctgat
    67201 agctgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt
    67261 ttacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt ttgtccgggg
    67321 cctgactttc tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    67381 taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg gtcacgctat
    67441 cagcattcgc cagattactg gccgccacgt tcaagcgctg ggactgggca gttaacgccg
    67501 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    67561 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    67621 tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct
    67681 ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt
    67741 gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga
    67801 cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat
    67861 tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt
    67921 gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct
    67981 gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg
    68041 ctatttatgc cgttgatgac cattgcggac aggtacaatt cacgttgtag aaatggctgg
    68101 gggcgaaaat gctggcaata aaacgtagcg tggcgatcat ctcgatacta ttcactccgt
    68161 taagtgcggc gagtaatctc acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg
    68221 gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccgtgcg
    68281 agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg
    68341 cacgctgcgg taacgacaaa cgatatttac aggttaatgt acaggccaca ggaaattatg
    68401 tggttgccgc gatgcccatt gtgcggggag gaaagctgga agctggcaat gtcaaactaa
    68461 aacgcggacg gcaggatacc ctgccaccgc gtacggtgca ggatatcaat caacttgttg
    68521 atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc
    68581 aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt gacgggttta
    68641 gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc
    68701 gcatggtatc gggactggtg gtcagcggcg ttgttgatgc agatgggaat attcttataa
    68761 accagtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa
    68821 gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcccgtaa
    68881 gcaccgttca accacgcgaa accactgacg cgtcggtaac gaacacccgg gcggcaaaaa
    68941 caacagcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    69001 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    69061 gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gctcaacgaa gcgcagcaag
    69121 acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct
    69181 gtgcttaacg atctcaaaac ggtaatggat caagaacagc aacatctctc tatggggcag
    69241 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    69301 gattacctcg aacagttacg caggaaagaa cccaatacag caaatagcgt tgatattagt
    69361 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    69421 ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    69481 catcaggaac cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt
    69541 aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta
    69601 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    69661 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc
    69721 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    69781 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    69841 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    69901 tgacagccca gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    69961 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    70021 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    70081 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    70141 cggacccgaa agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg
    70201 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    70261 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat
    70321 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    70381 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    70441 tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    70501 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    70561 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    70621 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    70681 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    70741 tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac
    70801 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    70861 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    70921 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    70981 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    71041 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    71101 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga cccacgggca
    71161 aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg
    71221 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    71281 tacgttgcgc cagcactgcc tgttcgccgg cggtttgcag aactgtctga ttttgcacac
    71341 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac
    71401 caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg
    71461 taatgtcgat gagcgcatcg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    71521 ggcgatgcat acaggtggtg atctgcaaag gatcggccga aaaatggtgc tcggcaaaca
    71581 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    71641 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    71701 cgtgtggccc gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga
    71761 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    71821 tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca
    71881 gcggtttatc gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt
    71941 ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg
    72001 aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg
    72061 accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    72121 gcgcaatgcc gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga
    72181 tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg
    72241 acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc
    72301 tctacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    72361 agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccact
    72421 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    72481 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga
    72541 tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca
    72601 ctcaccgtgc gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat
    72661 tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca
    72721 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc
    72781 acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    72841 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    72901 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    72961 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    73021 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    73081 cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac
    73141 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    73201 atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc
    73261 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag
    73321 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    73381 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    73441 aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc
    73501 attttctgca ttattcacat atttgataaa aactctctat caaagcgatg ttttgataaa
    73561 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    73621 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    73681 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    73741 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    73801 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    73861 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    73921 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    73981 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    74041 acgttgtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc
    74101 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    74161 tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    74221 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    74281 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    74341 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    74401 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    74461 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    74521 catggtcagc ggtctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    74581 cattgccgag cctgcgcgtg aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg
    74641 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    74701 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    74761 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    74821 cgcaacgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    74881 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    74941 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    75001 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    75061 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    75121 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    75181 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    75241 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    75301 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    75361 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact
    75421 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    75481 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    75541 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    75601 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    75661 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    75721 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    75781 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    75841 tacccggcgt ggctgacgcc catattgttc tgacaaactt gatcagccaa attggtcgcg
    75901 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    75961 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    76021 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    76081 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    76141 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    76201 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    76261 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    76321 ttgtgtccgg caaaaacatt ccttcagccg gagcatagag attaatgact gcaccatccc
    76381 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    76441 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    76501 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg
    76561 ccgtacctgc cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    76621 atcctaatga gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc
    76681 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    76741 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    76801 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    76861 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    76921 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    76981 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    77041 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    77101 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    77161 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    77221 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatgggt
    77281 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    77341 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    77401 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt atttaacctg tataaataac
    77461 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    77521 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    77581 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    77641 attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    77701 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    77761 ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa
    77821 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    77881 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    77941 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    78001 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    78061 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    78121 gaccccaaat aagtccgagc aggaagggac tgaagttggt caaacgctct ggttaaccac
    78181 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    78241 tgatttccag gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    78301 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    78361 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    78421 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    78481 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    78541 ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg
    78601 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    78661 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    78721 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    78781 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    78841 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    78901 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggttg acggtgagta
    78961 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct
    79021 gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    79081 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    79141 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    79201 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    79261 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    79321 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    79381 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    79441 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    79501 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    79561 cggctgttgc actatctca
//
LOCUS       DAJTVJ010000003        70303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000003 DAJTVJ010000000
VERSION     DAJTVJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.003"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..675)
                     /locus_tag="OO448_000170"
     CDS             complement(<1..675)
                     /locus_tag="OO448_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCR6335501.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHF"
     gene            complement(697..2709)
                     /gene="hyfR"
                     /locus_tag="OO448_000171"
     CDS             complement(697..2709)
                     /gene="hyfR"
                     /locus_tag="OO448_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6335502.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2739..3152)
                     /gene="hyfJ"
                     /locus_tag="OO448_000172"
     CDS             complement(2739..3152)
                     /gene="hyfJ"
                     /locus_tag="OO448_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6335503.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3145..3903)
                     /gene="hyfI"
                     /locus_tag="OO448_000173"
     CDS             complement(3145..3903)
                     /gene="hyfI"
                     /locus_tag="OO448_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6335504.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3900..4439)
                     /gene="hyfH"
                     /locus_tag="OO448_000174"
     CDS             complement(3900..4439)
                     /gene="hyfH"
                     /locus_tag="OO448_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6335505.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4449..6164)
                     /gene="hyfG"
                     /locus_tag="OO448_000175"
     CDS             complement(4449..6164)
                     /gene="hyfG"
                     /locus_tag="OO448_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR6335506.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6154..7734)
                     /gene="hyfF"
                     /locus_tag="OO448_000176"
     CDS             complement(6154..7734)
                     /gene="hyfF"
                     /locus_tag="OO448_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6335507.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7739..8389)
                     /gene="hyfE"
                     /locus_tag="OO448_000177"
     CDS             complement(7739..8389)
                     /gene="hyfE"
                     /locus_tag="OO448_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6335508.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8401..9753)
                     /gene="hyfD"
                     /locus_tag="OO448_000178"
     CDS             complement(8401..9753)
                     /gene="hyfD"
                     /locus_tag="OO448_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6335509.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9729..9839)
                     /locus_tag="OO448_000179"
     CDS             complement(9729..9839)
                     /locus_tag="OO448_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR6335510.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9856..10803)
                     /gene="hyfC"
                     /locus_tag="OO448_000180"
     CDS             complement(9856..10803)
                     /gene="hyfC"
                     /locus_tag="OO448_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6335511.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GALITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10814..12832)
                     /gene="hyfB"
                     /locus_tag="OO448_000181"
     CDS             complement(10814..12832)
                     /gene="hyfB"
                     /locus_tag="OO448_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCR6335512.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12832..13449)
                     /gene="hyfA"
                     /locus_tag="OO448_000182"
     CDS             complement(12832..13449)
                     /gene="hyfA"
                     /locus_tag="OO448_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6335513.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13702..14172)
                     /gene="bcp"
                     /locus_tag="OO448_000183"
     CDS             complement(13702..14172)
                     /gene="bcp"
                     /locus_tag="OO448_000183"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6335514.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14172..14744)
                     /locus_tag="OO448_000184"
     CDS             complement(14172..14744)
                     /locus_tag="OO448_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6335515.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14890..15768
                     /gene="dapA"
                     /locus_tag="OO448_000185"
     CDS             14890..15768
                     /gene="dapA"
                     /locus_tag="OO448_000185"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6335516.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15785..16819
                     /gene="bamC"
                     /locus_tag="OO448_000186"
     CDS             15785..16819
                     /gene="bamC"
                     /locus_tag="OO448_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6335517.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17032..17745
                     /gene="purC"
                     /locus_tag="OO448_000187"
     CDS             17032..17745
                     /gene="purC"
                     /locus_tag="OO448_000187"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6335518.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17913..18761
                     /locus_tag="OO448_000188"
     CDS             17913..18761
                     /locus_tag="OO448_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6335519.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18776..20791
                     /gene="tmcA"
                     /locus_tag="OO448_000189"
     CDS             18776..20791
                     /gene="tmcA"
                     /locus_tag="OO448_000189"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6335520.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20865..21563
                     /gene="ypfH"
                     /locus_tag="OO448_000190"
     CDS             20865..21563
                     /gene="ypfH"
                     /locus_tag="OO448_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6335521.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21673..21873)
                     /locus_tag="OO448_000191"
     CDS             complement(21673..21873)
                     /locus_tag="OO448_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6335522.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21901..23028)
                     /gene="dapE"
                     /locus_tag="OO448_000192"
     CDS             complement(21901..23028)
                     /gene="dapE"
                     /locus_tag="OO448_000192"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6335523.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23032..23388)
                     /locus_tag="OO448_000193"
     CDS             complement(23032..23388)
                     /locus_tag="OO448_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6335524.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23770..23829
                     /gene="ypfM"
                     /locus_tag="OO448_000194"
     CDS             23770..23829
                     /gene="ypfM"
                     /locus_tag="OO448_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6335525.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23928..27041)
                     /gene="acrD"
                     /locus_tag="OO448_000195"
     CDS             complement(23928..27041)
                     /gene="acrD"
                     /locus_tag="OO448_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6335526.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27205..28905)
                     /gene="narQ"
                     /locus_tag="OO448_000196"
     CDS             complement(27205..28905)
                     /gene="narQ"
                     /locus_tag="OO448_000196"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR6335527.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29111..31090
                     /gene="aegA"
                     /locus_tag="OO448_000197"
     CDS             29111..31090
                     /gene="aegA"
                     /locus_tag="OO448_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6335528.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31158..31733
                     /gene="nudK"
                     /locus_tag="OO448_000198"
     CDS             31158..31733
                     /gene="nudK"
                     /locus_tag="OO448_000198"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6335529.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31859..32902
                     /locus_tag="OO448_000199"
     CDS             31859..32902
                     /locus_tag="OO448_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6335530.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32998..35001)
                     /gene="tkt"
                     /locus_tag="OO448_000200"
     CDS             complement(32998..35001)
                     /gene="tkt"
                     /locus_tag="OO448_000200"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6335531.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35021..35971)
                     /gene="tal"
                     /locus_tag="OO448_000201"
     CDS             complement(35021..35971)
                     /gene="tal"
                     /locus_tag="OO448_000201"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6335532.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIARGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36260..38539
                     /gene="maeB"
                     /locus_tag="OO448_000202"
     CDS             36260..38539
                     /gene="maeB"
                     /locus_tag="OO448_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6335533.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38832..39167
                     /gene="eutS"
                     /locus_tag="OO448_000203"
     CDS             38832..39167
                     /gene="eutS"
                     /locus_tag="OO448_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6335534.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39180..39659
                     /gene="eutP"
                     /locus_tag="OO448_000204"
     CDS             39180..39659
                     /gene="eutP"
                     /locus_tag="OO448_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6335535.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39634..40335
                     /gene="eutQ"
                     /locus_tag="OO448_000205"
     CDS             39634..40335
                     /gene="eutQ"
                     /locus_tag="OO448_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR6335536.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40332..41135
                     /gene="eutT"
                     /locus_tag="OO448_000206"
     CDS             40332..41135
                     /gene="eutT"
                     /locus_tag="OO448_000206"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR6335537.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41132..42148
                     /gene="pta"
                     /locus_tag="OO448_000207"
     CDS             41132..42148
                     /gene="pta"
                     /locus_tag="OO448_000207"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6335538.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42187..42480
                     /gene="eutM"
                     /locus_tag="OO448_000208"
     CDS             42187..42480
                     /gene="eutM"
                     /locus_tag="OO448_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR6335539.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42587..42874
                     /gene="eutN"
                     /locus_tag="OO448_000209"
     CDS             42587..42874
                     /gene="eutN"
                     /locus_tag="OO448_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR6335540.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42886..44289
                     /locus_tag="OO448_000210"
     CDS             42886..44289
                     /locus_tag="OO448_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR6335541.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44300..45136
                     /gene="eutJ"
                     /locus_tag="OO448_000211"
     CDS             44300..45136
                     /gene="eutJ"
                     /locus_tag="OO448_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR6335542.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45126..46313
                     /gene="eutG"
                     /locus_tag="OO448_000212"
     CDS             45126..46313
                     /gene="eutG"
                     /locus_tag="OO448_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCR6335543.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46630..47856
                     /gene="eutH"
                     /locus_tag="OO448_000213"
     CDS             46630..47856
                     /gene="eutH"
                     /locus_tag="OO448_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCR6335544.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     CIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47853..49256
                     /gene="eutA"
                     /locus_tag="OO448_000214"
     CDS             47853..49256
                     /gene="eutA"
                     /locus_tag="OO448_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6335545.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49268..50629
                     /gene="eutB"
                     /locus_tag="OO448_000215"
                     /pseudo
     CDS             49268..50629
                     /gene="eutB"
                     /locus_tag="OO448_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50650..51537
                     /gene="eutC"
                     /locus_tag="OO448_000216"
     CDS             50650..51537
                     /gene="eutC"
                     /locus_tag="OO448_000216"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6335546.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51547..52206
                     /gene="eutL"
                     /locus_tag="OO448_000217"
     CDS             51547..52206
                     /gene="eutL"
                     /locus_tag="OO448_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6335547.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52219..52719
                     /gene="eutK"
                     /locus_tag="OO448_000218"
     CDS             52219..52719
                     /gene="eutK"
                     /locus_tag="OO448_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6335548.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52765..53817
                     /gene="eutR"
                     /locus_tag="OO448_000219"
     CDS             52765..53817
                     /gene="eutR"
                     /locus_tag="OO448_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6335549.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="OO448_000220"
     CDS             complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="OO448_000220"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6335550.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="OO448_000221"
     CDS             complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="OO448_000221"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6335551.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55809..56234
                     /locus_tag="OO448_000222"
     CDS             55809..56234
                     /locus_tag="OO448_000222"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6335552.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56221..56670
                     /locus_tag="OO448_000223"
     CDS             56221..56670
                     /locus_tag="OO448_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6335553.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56731..57306
                     /locus_tag="OO448_000224"
     CDS             56731..57306
                     /locus_tag="OO448_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6335554.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57402..58301
                     /gene="yfeX"
                     /locus_tag="OO448_000225"
     CDS             57402..58301
                     /gene="yfeX"
                     /locus_tag="OO448_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6335555.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="OO448_000226"
     CDS             complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="OO448_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCR6335556.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59668..59841)
                     /locus_tag="OO448_000227"
     CDS             complement(59668..59841)
                     /locus_tag="OO448_000227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCR6335557.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59854..61092)
                     /gene="murP"
                     /locus_tag="OO448_000228"
                     /pseudo
     CDS             complement(59854..61092)
                     /gene="murP"
                     /locus_tag="OO448_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="OO448_000229"
     CDS             complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="OO448_000229"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6335558.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62156..63013
                     /gene="murR"
                     /locus_tag="OO448_000230"
     CDS             62156..63013
                     /gene="murR"
                     /locus_tag="OO448_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6335559.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63142..63933
                     /gene="ucpA"
                     /locus_tag="OO448_000231"
     CDS             63142..63933
                     /gene="ucpA"
                     /locus_tag="OO448_000231"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6335560.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64104..65120
                     /gene="cysP"
                     /locus_tag="OO448_000232"
     CDS             64104..65120
                     /gene="cysP"
                     /locus_tag="OO448_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6335561.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65120..65953
                     /gene="cysT"
                     /locus_tag="OO448_000233"
     CDS             65120..65953
                     /gene="cysT"
                     /locus_tag="OO448_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6335562.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65953..66828
                     /gene="cysW"
                     /locus_tag="OO448_000234"
     CDS             65953..66828
                     /gene="cysW"
                     /locus_tag="OO448_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6335563.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66818..67915
                     /gene="cysA"
                     /locus_tag="OO448_000235"
     CDS             66818..67915
                     /gene="cysA"
                     /locus_tag="OO448_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6335564.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68049..68960
                     /gene="cysM"
                     /locus_tag="OO448_000236"
     CDS             68049..68960
                     /gene="cysM"
                     /locus_tag="OO448_000236"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6335565.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68963..69331)
                     /locus_tag="OO448_000237"
     CDS             complement(68963..69331)
                     /locus_tag="OO448_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6335566.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69436..70287
                     /gene="pdxK"
                     /locus_tag="OO448_000238"
                     /gene_synonym="thiJ"
     CDS             69436..70287
                     /gene="pdxK"
                     /locus_tag="OO448_000238"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6335567.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWGVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16699 a        18349 c        19439 g        15816 t
ORIGIN      
        1 gaaatggcga atggcaatgg cgaacggaat cacaaacaaa ttggcgatac agtgctcaaa
       61 cccactggcg acaaacaggg tgatgggcaa aatcatggcg acgattttgt cgcataatga
      121 acgcccgcaa taactcatcc acagcgccag acaaaccatc agattgcaca taatgccgag
      181 gctgacagat tcagtaaatg tatgatgcat tttgccctcg gcgcagtgta aaaccgcgac
      241 tccccacatc gcgttttcac tcatcaccag cccggaaaac cagattaaca aactgaaaca
      301 ggcaatacct gccagattac cgcaggccac cagaagtgcg ttaatcagcc aagttcgcca
      361 actaataacg ccccgacttt ttgccatcac cgtcattacc gacgaggtga acaggctggt
      421 gccacaaacc gccagcaaaa taaagccgag tgtaaagcaa aggccgccca ccagatgggt
      481 taatgcctgt gaagacgggg catcggcgat aacggaaagg taaaaagtaa atccaatcgc
      541 cataaacacg ccagcggaca tcgccattaa aaagaccgac actttgctgc gggcaatttt
      601 atgggcagta atccgttcag cgattgccgc ttttctggcg ctcaactgca agtcgaaaga
      661 gagtttgttt ctcatgcata ctcctgcaaa agcagattac aacacctcgc gaaccgagag
      721 ccccagacgc tgcattcgtg acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc
      781 gccacggggc ccggcaacaa tgccattggt ttctcgcaat acctgaacaa tgcgctgacg
      841 ttcttcttcg tcattctctg gcgtcgtcgg gtgcagcaac tgcgccattg aactgcgaag
      901 cgctgaatct tcatttagcg tcggcagtaa atggctttgt cggacattaa gatgtaaatt
      961 cagactgtta ccgcgagtca gcagtaccgc tcgctcaatc acgttttcca gctcgcgcac
     1021 gttgcccggc cagtcccacg acatcaactg gcgtagcgcc tcggtcggga tggcgtcaat
     1081 tgaacgattc atatggcgcg ccattttttg cgtgaaatgc tttgctaaaa gagggatatc
     1141 ttccggacgg tcgcgtagcg gcggcaattc cagtgggaag acattcaggc gataaaagag
     1201 atcgctgcga aactggcgat cttcaaccat ttgccacaaa tcacggttgg tggcggcaat
     1261 gactctgaca ttcaccggga tcgttctact cccgccgaga cgctcaatct cccgctcctg
     1321 caatacgcgc agcagtttag gctgaagttc taacggcaga tcgccaattt catcaagaaa
     1381 caacgtgccg ccatcggcaa tttcaaaacg accacgatgg gtattaatcg caccagtaaa
     1441 cgcccctttg tcatgaccga ataactcact ttccagaaga ctggcgggga tggcagcgca
     1501 gttgattttg accagcggct tgtcgcgtcg cgggctaagt tgatggatcg ctctggcgat
     1561 cacctctttg ccggttccgg tttcaccaca aatcaacact gtactgtcgc tcttcgccac
     1621 aatatctacc tgctggagca ggtcttccat tgcctggctt tgatagataa tgtcaccgac
     1681 gcccagattc gaaaggagct gctcgctaag ctggtggttt tcttgctgca aactttcctg
     1741 caaatcggtc atgctacgcc aggcatcggc attgccaacg gcaatagcga tgcgatcggc
     1801 tatgtgttgt agtagctgac agttttcctc actaaagaga gtggaagagg tatgcgccag
     1861 caacaatgca cccggtgtat ggttgccaaa ggtaagcggt ataaggagcg cagattcgca
     1921 gccgttagat gcaagtaaga gtaataacgg gtcgcgttgc cagagaaaca gatcgtctgc
     1981 acggtgcgtc aaggtcggtt gtcgggtttg tagcgtttgt gtcagcaata cactgtcgcc
     2041 aggcatattg cgtggcagac acgcacaatg tgaggcagaa agatcgctgc accacaggct
     2101 aaacttctcg ttctttcgat gatcgcccag taccatactg actgaagcca gaccgaaaaa
     2161 atgatggatc tcacgagcga cgtcagcgat cagatcgtcg agatcaagat gtgacagcac
     2221 tgcattagta atatcaacca gtacccgatg atgatcgcga tctttctcca gcaaacgcag
     2281 cgccacctgc tgttcttcat cattgagcca tgattgcaga accggggtga ttatccgcgc
     2341 cagccagtta aacagtgtgc aatcctgggg agtaaaacgg cagccgtggg tgcgaataaa
     2401 gcgtagctcc gccattccac tactgccgag aaggacggcg tgacgcgaat aatcgaccat
     2461 ctcgccctga tactctgttg ttgccgtcac atcgctgcca tcatcatggt agcggtaata
     2521 gaagttgctt ttgagaacat gcagttcaat aagttgtacc gggctgaacg gcggcggcag
     2581 cgggatgaag gcgcgtaata aagacgcctt accgtgtttc tgcgctaacc gctccgcgag
     2641 cattccgttt accgcttcaa ttgttattcc ttgcggcggg gcaaacatcg cctcgtctga
     2701 catagccatc aacagtcctt attaatgaga aatccttctc acgcaatttt tctggccatc
     2761 agatagatgg ccggttcctg cacaatttca tccaacagat gaatgaattg aatgctacgc
     2821 gcctgccaca cagacgtcgc gtcatccgcc agtggcgcga atgcccgggt caacagggcg
     2881 gtgtggctgg cgtcaataac aatctcacca aaagtcatca cccccagcat cttacgtcgg
     2941 gcttgctcct cttcgaccag tgcaagccaa tcttcgtatt ccgtcagtgg gcaggggaag
     3001 gcgacattca gacaatcaat cacgccaacg tgatgaccga tagccagcga gtaatacatt
     3061 acctgagagc tgtgttccgg catctcgtcg ctactggcga caaacttttt tcgcagcgtc
     3121 cagaaaacaa tttccccgca ctcttcagtc atgtaacccc cggagtacgc gaaagagttg
     3181 ctgaacgata ctattgagac gtggatcgtc ggcatcgcgc aaccaggtgt taacccgatt
     3241 tcctgtagga tcgtcgctta aatggcgcag gagccgatcg caaatttctc gcccctgacg
     3301 atagcccgcc agccgccgcg cttctcgctc aatggcgata cgctgtgatg gcgggatctg
     3361 cggccacaac ggttgcatag tcaccccggt gggatcgcga taatccaccg cgtgaatctt
     3421 ctgttgcagc aaaccgagcg ccacggcgaa accgtgaatg gtggcggccg gtgttggcgg
     3481 gcagccgggg atccaaacat caatggggac aatggtgtcg ctgccgcccc agacgctgta
     3541 gagatcgtgg aaaataccgc cgccgacacc gcacgcgccg taggaaacac aaattttatg
     3601 atcgggggca gactcatacg cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc
     3661 tgtaaataac aaaatatcgg cgtgacgcgg tgatgaaata accttaatgc caaaacgttc
     3721 tgcgtcgaat actggtgtga tggcggcaaa aatttcgatt tcacaggcgt tgcagccgcc
     3781 gcaatcgacg cgataaacgt aagccgagcg acggatatcc tgtagcaaat gctgcttcat
     3841 cttttgcgtt tgctcgtcca gcgtgatggg ctggctgaca tgttgtgtaa gcactggact
     3901 catagctgct ccttagccac cagcggtaca tctgtatcgt cgttgagcag cgtcgcgcgt
     3961 tgtttgcact ccgggcagac gctcgcttgc gcccataaca tttcgcggtt ttgtggcgca
     4021 ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg ttttttgcgg ggcaaacggg
     4081 cgttcgcaac ggctgcaacg ttgtagatgg aacgtcgcgc gggtatagag atcggcttta
     4141 ttggtgacgg tcagttcaaa gttattggta agctggatgg ctctggtcgg gcacacttct
     4201 tcacaacgtc cgcagtaaat acaacgcctc agatagagct gccaggtgcg cgaattttgc
     4261 tggtcgtcgg tctggatagt cagcgcattt gccggacaag cacaggcgcc gcaggcaata
     4321 cattggctgg gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc cagtggcgcg
     4381 aagggatatt tcaccgtcgc ggttccggcg cgcataatag ttttcagtaa cttcagcatc
     4441 ttctgtcctt acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc gatctcttta
     4501 tacggcacgg tttttgactg gcgcttgcgc acatctacca gcgtcacacg gtcggtacag
     4561 gagtagcagg gatcaaggct accgataatc agcggtgcgt cagaaacggt attgccgcgc
     4621 agcatgtaac gcaacaccgg ccagttggcg taggtggcgg cacggcagcg ccagcggaac
     4681 aatttttggt tatcaccgag catgctccag tgcacgtctt caccgcgtgg cgcttcaaca
     4741 aagcccagcg cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag tggggtatcc
     4801 ggcatgttgt cgagggcaaa ttccagcatt gccagcgaat caaacgtctc tttgacacgg
     4861 accatcacgc gggagaaaac atcgccgccg gtaaaggtaa acagtgtttt tggaatattg
     4921 ccgtagtcgg cgtaggggtg atcaaagcgc aaatcacggg caaaaccact gccgcggatc
     4981 agcggcccta cagggctata atcacgggcg atttgtcggt cgagaatgcc aatgccctga
     5041 gtgcgttgtt ccatattcgg cgtggcaagc agcatctcta ccagctccga cacgtcggcg
     5101 cgcatctcgc gcaccagttt cagcgtttgc agacgttgct ctttgagaat atcgcggcga
     5161 acaccaccaa tcagattcag accgtaggtt ttacgcgacc cggtcagcaa ttccgccatt
     5221 gtcatcgact tttcccgcac gcggaaaaat tgcataaagc cggtatcaaa tccaacaaaa
     5281 tggcaggaga ggccaaggtt gagcaaatga ctgtgcagac gttcgacttc cagcagaatc
     5341 gagcgaatag tatgcgctcg ttgcggcacc tcaatcccca gtgcattttc aaccgagttg
     5401 gtataggcca cactgtgggc aaaaccgcaa atcccacaca cgcgatccga taagaaggtc
     5461 acttcgttgt agcccatccg cgtttctgcc agtttctcca tgccgcgatg gacataaaac
     5521 aggcggtaat cagcatcgac aatttgctcg ccatccacaa acaagcggaa gtgacccggt
     5581 tcatcggagg tgatatgcag cgggccaata gggatcaccc gcgcatcgct attgccctca
     5641 ttgataaacg gatacgtttc ggtatcagtc gtcggttcag ggcgcagtcg ataatccatc
     5701 gcatctttgc gcagcggatg catatcttcc ggccagttat cgggcaacac caggcgacgc
     5761 tgatccggca ggccaaccgg aatcagcccg tacatatcgc gaatttctcg ctcgccccag
     5821 accgcggcag ggacgcgcgg tgtgactgac ggaaactccc gactgtcggc atcgaccagt
     5881 gccttcacca cgatccagca tttttcggcc ccttccattg aaagggcata ataaaccgcg
     5941 taatgaccgt taagtgtccg ctcgtcgttg ccaaacagga ctggaagcca gccatcatgt
     6001 tgataataaa gataatgtac aacgtcaggc agcagattga ttttcaccgt aatggtgacc
     6061 tgttcaggcg tttgtcgctc ttcatccagc accgcgccgg ggaactgcgt tttcagggcg
     6121 gcgagaatcg cttcgccacg atttgacgat gaattaacgt tcactgtgtt tctccgaaac
     6181 agatgcggtg cctgaaggca aaaaatcatg ccaggtgcta cgttgtgcag gcaggtcgtg
     6241 cgtccctgag aggactatag tggaagcgcc cgccaggatc ctgatgacag gttgtggaat
     6301 atgcgttccc atcgccagca tcatgaccag cagaatcacc attggcgagg tggtcaacca
     6361 gccgagatca ccccggttaa cggcctgcgg cggtttcgcc attaacaccc gcgctgccat
     6421 ccgtaccagg cccgccagca ccagcgttaa cagcaataac agcaggacga taatcagcag
     6481 gtgattacgt gccagtccgg cggtaatggt cataaattcg ctaagaaaaa tgttgaaggg
     6541 cggcatccct gccagcgcca gcgcaccgcc gccaaacagc acggcggtaa atggcatgat
     6601 tttgagcatc ccacagacga cgttgagatc gcgcgtgccg tacttgagca gtacattgcc
     6661 ggaaccgcag aacagcagcg tttttgccag actgtggttt aagatgtgca gcagcgcggc
     6721 aaaaattccc agcggcccgc caatgcctag cgccaccgcg accagcccca tgttctccac
     6781 gctggagtac gccagcagac gcttaatgtc ccgctgtacc agaatgaaaa atgccgccac
     6841 ggcaaccgac aacatgccga agatgagcaa caaccggttg gggaaatcgc tgccgatggc
     6901 ttggcaaata atgatgtaat agcgaatcag caccaacagc gcgcagttca gcaatacggc
     6961 ggagagcagg gcgctgaccg gactcggcgc ttcactgtga gcatccggca gccaggcgtg
     7021 catgggaaat agcccggttt tggtaccaaa gccaattagc acaaacacaa aggccaacag
     7081 cattaatgtt gggtcaagca aggacgattg cttaagaacc tcgctccaga atatcgccat
     7141 ttctgcctgc ggcataacgc tggcggcgtt ggcgtatacc agcacggtac cgaacagacc
     7201 aaaagcgaca ccaacagtac aaataatgat gtacttccat gctgattcca gcgatgaacg
     7261 ctgaccgtaa atgcctacca gaaacgccga gcttaaggtg gtggcttcga tcgccgccca
     7321 catcacaatc aggttattgc tggtaacaac cagcagcatg gtgaacaaaa acagatggaa
     7381 gaaaccgtag taatcgcaca gcgtaacggg tgaaagctcg ccgtgtgcca cttcatgacg
     7441 catgtagcca atcgagtaaa caccggtgag aaagccaatc acaccaagaa tggcgaggaa
     7501 caaaccgccc agaccatcaa tatgaagcca cagtcccgca gcgaatattt ctcctgcatc
     7561 agcggcagtt tggaccaccc agagtgccag aatcagcagc agtgtgatcc ctaagctatg
     7621 taatacagtc accgtacaag tcgcagagag cccccgtttc cggcaggcaa aacagagcag
     7681 cgaaaaaagc agcggcgtga gcaggagtaa agcgaacatc acagaataac tcatctcatt
     7741 atcccttcag cgcggtcaag ttgttcacgt ccagcgtgcc gtgggtacgc catatttttc
     7801 ttgccagtaa caccatcaca atgacggcga agatggcgtc ggtagcgata cctatttcca
     7861 ccagttccgg tgctcgccag gcaagaagcg ccagcaccag atgggagccg ttttccatca
     7921 ggcagtaacc aaaaatttgc cgcaggatat tgcgctggct gacaatgcac agcaggccaa
     7981 gcagaaaatg acctaacgct accgccagcg ccggtttcag cccggtagcc atcggtagct
     8041 tcacgggctg aacgacaaat gcgcaaagca ggacaattaa cgccgcgagc agtgccatca
     8101 ttgccggacc gaataacgct ttttccggga tgttctgggg aatatttcgt gcagcgtaag
     8161 tcatgattaa cggtaccagc agcactttgg tgataaaggc gctggcggac cagatcagca
     8221 gttgctctgc ggcgaacagg cacgagagag tggcgaaaat agacaccagc accagcgact
     8281 ggcaggcgta aaatccgcat gacaggcgat agcttttgac gctaatcaca aacagcgatg
     8341 tcagcatcat cagtcccgcc agattattta cgatcataga accggtcatt catcatctcc
     8401 ttactgcaac caggtcgcgg cgattacgct ggaaatcagc gacatcacaa tcagtacaat
     8461 caacaccagg cgcattgacc ctggcagcgg tgcggcatcg gcgacggcct cgctcggttt
     8521 gcccggcacg acgcgaccaa accagcgaat aaaccaggcg aaactggcga ccgattcaat
     8581 catcagcaga atcatggcgg gcagcaggat ccagtactcc actgacaacg caaaaccgac
     8641 ggcaaacagc gggaatttac tgaagaagcc gttgaacggc ggcacaccgg taatcgccag
     8701 cgctgccacg cagaaaccca cgcttggcaa cggcagggtg tgcaatacgc cacgcagacg
     8761 cggcaacaag cgcgtgccgc agctgtaact cagcgcacct gctacaagga aaaacaggct
     8821 tttagcgaac gcgtggttga cgatgtaggc gatgctaccc tccagcgcca gccgcgagcc
     8881 gaagatggag agcgacaagc cgaagaacat ccagccaagt tgagtgatgg tcgaccaggc
     8941 cagcaaccgc ttcatatcct gctgtggcaa atacatcaga aagccataaa gaatggtgac
     9001 cagcgccatg accatgccaa cgccgccaat cacatgcggg atattgccgc cgtcgataat
     9061 ggcgcgggca aaaatgtaaa cgcccacttt caccatcgat gcggcgtgga gataggcact
     9121 gatcggtgtt ggcgcttcca ttgcatccgg tagccacgct tgcatcggta gctgggccga
     9181 tttcccccac gcggcaaaca ggatgccgcc ataaaccaga taacgtgcgt cgccgtgtaa
     9241 ctcgctcatc gcgctaagcg caaacgttcc ggtctgcaaa aacagcgtgg cggcggcaag
     9301 atacaacccc aacgagccga tatgagtgat aagtaacgct tttagtgctg aaagctgtgc
     9361 tttatcgctc tggtaataac tgatcaacgc ccaggagcag ccgcccgtaa tttcaaaaaa
     9421 caacaactga ccgagcagcg tcgaggagag taccagtccc gccatcgcgc cgataaacac
     9481 cagcaaaaat gcgtaataac gattcgtgcc gttatgcggg tgttcgcgat ttttatccgt
     9541 cagataaccc gtggagtaga tcgtgaccag caaaccgaga aacaccacca caaacagaat
     9601 cagcgtactc atgcgatcaa tgaccaagcc aaacaatgcc acatccccca cgttgaccaa
     9661 cgtcaacgtg acggcaactt tatcggcctg ataaaacgcg gagatcagcg acaacatgca
     9721 cagcgtggtc agtgcggcga acaaaacccc cattcggcgg cccgacgttg tggcgaaaac
     9781 gaaacgacca gtgcgccgat aaaaggcagc attaacgtcg tcagagcaag attttccata
     9841 ttccgtcagt gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct
     9901 gaagccaagc caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc
     9961 tgcgattgag gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt
    10021 cagcaggcag gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa
    10081 cagtgctgcc atcacgacct gtttcagccc cagcccccat ttcgctagcg ccagcccggc
    10141 accggaatat tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa
    10201 gggaattttt cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt
    10261 ggttagcggc gagttccagc ccgttgccag cgtattgctg atcatctcga tatgcgtgga
    10321 acctgctatc agcgccaata ccagcagtga gagaatgagc atgggttcga ccagaatgcc
    10381 gagcgtcaac tcgcgactgg caccgactcc cgcaaacggg cttccggtat ccagccctga
    10441 aagagcaaag aaaaaacgaa acagggcaag aagatagata agggtgatca gagcgccgcc
    10501 gcccgcaaaa ggggaaacgg taataaatag tggtagggcc atcgccagca ccagcatgct
    10561 gctgattaat acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcatcttc
    10621 ctggcgttta aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg
    10681 ggagtgcata cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag
    10741 cagaatcacg gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct
    10801 catggtgatt tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat
    10861 acaggcaata gagacgaaag tcgccgcttt gcagatgctg tatttctttg gccagccgtt
    10921 gggtggcgct cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag
    10981 ccctggcggt gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc
    11041 gtagacgaaa tagcgcagaa aagaccacac gcagcggctg catcacgcca ttgcctgacg
    11101 gggccatcgc attttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg
    11161 ctgcgcggcg cgaacgacac cacagccagt aaaggccagg cagcaaaggc atcgccagta
    11221 gtaacagcaa cagtaatgaa ggagtgaccc gtgtatgaaa cgtgccgggt acaagtgcta
    11281 ttccgctggc gacagtgacg ggaggggtat tggtaaacgc atgggcaatg tgcattatct
    11341 tcggtgccag ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca
    11401 tggcgacgat catcgtattt ggcacttcct gtgcctcttc agcgtgtgtg ctgcgcggcg
    11461 caccacagaa agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga
    11521 ctgccagcat taccatagca ataggacccg caagttgtag cgctacggct tccacacgac
    11581 ttagtgagaa tagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa
    11641 tggctgatat cgccaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg
    11701 ccagtgcccc cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcacccgcgc
    11761 cgagaaatag cagccctttg aaaagcgcat ggttgagcag atgaaacagt gcgccgagca
    11821 gtccaaccac ggtgagcagc gggtcgtgca gtgacagacc gaccatcgcc acaccgaccg
    11881 ccagcaaaat aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct
    11941 gttccgccag tgcatacagc acgcccagga gcgcggagat tgcgccgatc gccatcacca
    12001 gaatgcccca ccacagcggc aaacccgttt gcgccagcag atccatcgcc actttcagga
    12061 tgccgaaaat accgattttg accattacgc cagacatcaa cgctgaagcg tgcgatggtg
    12121 cggcagggtg agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa
    12181 aaccgaaaaa ggccagcagg aacaccgccg acgccagccc cggagaaagt gaaagcgtgc
    12241 ggaaactggc aaaatcgagg ctgccgcttt cgcgccccat cagcaagaag gcgatcatta
    12301 tcagcaccga tccggcgtgg gcgataaaaa agtagagcat gccagcgttg atcgacgttt
    12361 tatcctgcct ggcaatgacc agaaaccagg aagacagcga catcatttca aacagcacga
    12421 tgaaccaaaa agcgttgtcc atcaccagca gggcaaccat cgatgcgatg aaaatattca
    12481 taaagaagcc catcgccgcc gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat
    12541 agagagaaca aaccaccacc agcaaggaga taaccagcac cataaatgcc gagaggctat
    12601 ccattcgcag ggttaaatcg gcaaattcaa aaggcgtggc aaaacgggcg actaaagtta
    12661 cgcctgcatg taattgcgta attccgctga tgatgccaat cacgccgccc accagcgatg
    12721 tgatgccgga aagcttaata gccagtctgt ccagaccgag taaaaacagc gaagccagac
    12781 tggcaaacag atagaggatc agcgaccagg ttaataattg cagggcatcc attagcgttg
    12841 ctcctgagtg agggaaaggg gatcctcccc gccattggca aaccagcttg cggcaaggcg
    12901 ctgtttttct ttcatctggc gttgcaggct atcgtcggtg atcagccgta acgcctgatt
    12961 cgggcaagcg cgaacgcagg ccggaccttc tggcaagaaa tcacacaggt cgcatttcac
    13021 cgcgacggtc tggacacccg gttcccagcg cagcaacgca tgttggggcg gcacgttttc
    13081 ttcgccgtct ttgagtgagc cctcagcctg aaaaacatat tgcgcagggg cattcaccgg
    13141 acggcttcct gaagcgctga ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc
    13201 aatacagagg ctttcgttga gttggatcgc atcatccctc tgagagatgg cattgaccgg
    13261 gcagacctgc aggcaagggg cttcctcaca gtgatgacac acgacagggg cagtgagtgt
    13321 tgacgtcttc gccagggcca ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc
    13381 cgaacaggca gcgagacagg tattacatcc tgtacaccac agtggttcgg ccaccacaaa
    13441 gcggttcatg tcggctcctt gaaaatgata tgaaacattg catttcatat tgcataaagg
    13501 atgctattct gattaactca atatattcaa taagttatgt attaactgga ttctgaacgg
    13561 tacaaaaatg atggtcatta acgttcagca aaaaatggat tcgacacccg tgtcgcaggg
    13621 gaaagcgaga atgtgatctt gatcacgaga agtgaggtat gctggccgca agcgcagcca
    13681 gcacggaatg gagcaaagta atcaggcgtg ttctttcagc cagttcagca caacgtcgtg
    13741 gtgattgctg gttttgaaat cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag
    13801 gaagctgatg cgatgaatgc catcgtaggt tttgcccatg aaggactttt caccccagac
    13861 gccgaattgt tcgcacacct ggtggtcctc atcagacagg agcgtaaagt taagcagctc
    13921 tttttccgca aaacgggaga gtttttcggg tttatcggtg ctgataccca gcacatcaac
    13981 gcccgctttt ttcaactcat ccatgttatc gcgtaagccg caggcctgta cggtacagcc
    14041 gggggtcatg gctttcgggt agaaataaac cagaacacgc tgtccctgga agtcggtcaa
    14101 attaacttgt tctccgtctt gatccggcaa gctaaatttc ggtgcgatat caccggcttt
    14161 cagtggattc attacttaac tccatcctgt tcatcatgtt gggaataatt gacgacgtta
    14221 atactgcctt gtgcattgag ttctgtacat agggctttga acgcttgctc aatatttgct
    14281 gcgtccgcag atgcggggct gtgggcggtt atctgaatat gcaactgcgc agcccgttca
    14341 ttttcagcag gttgcgtgcg cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa
    14401 agtgctgtga agcgttcaat taaatgcggg gagtctgcca catcgacctg aacccagaca
    14461 gatgctggca ttggcggacg cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc
    14521 agctcggcac ctttcaacgg taacgttgat tcaatcagag taatggcatt ccatgaaccg
    14581 gaaagcagca tgataaacgt gaactcttct cccagcatcg ccaggcgact gtcttcaata
    14641 ttacagccgc aactactgac atgacgggtg atggtgttca caattccagg gcgatcggca
    14701 cccaatgcag tgatcaccag ataatgttgc gatgacagtg tcaaactggt tattccttta
    14761 aggggtgagt tgttcttaag gaaagcataa aaaaaacatg catacaacaa tcagaacggt
    14821 tctgtctgct tgcttttaat gccataccaa acgtaccatt gagacacttg tttgcacaga
    14881 ggatggccca tgttcacggg aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt
    14941 aatgtctgtc gggctagctt gaaaaaactg attgattatc atgtcgccag cggtacttcg
    15001 gcgatcgttt ctgttggcac cactggcgag tccgctacct taaatcatga cgaacatgct
    15061 gatgtggtga tgatgacgct ggagctggct gatgggcgca ttccggtaat tgccgggacc
    15121 ggcgctaacg ctactgcgga agccattagc ctgacgcagc gcttcaatga cagtggtatc
    15181 gtcggctgcc tgacggtaac cccttactac aatcgtccgt cgcaagaagg tttgtatcag
    15241 catttcaaag ccatcgctga gcatactgac ctgccgcaaa ttctgtataa tgtgccgtcc
    15301 cgtactggct gcgatctgct cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt
    15361 atcggaatca aagaggcaac agggaactta acgcgtgtaa accagatcaa agagctggtt
    15421 tcagatgatt ttgttctgct gagcggcgat gatgcgagcg cgctggactt catgcaatta
    15481 ggcggtcatg gggttatttc cgttacggct aacgtcgcag cgcgtgatat ggcccagatg
    15541 tgcaaactgg cagcagaagg gcattttgcc gaggcacgcg ttattaatca gcgtctgatg
    15601 ccattacaca acaaactatt tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag
    15661 gaactgggtc ttgtggcgac cgatacgctg cgcctgccaa tgacaccaat caccgacagt
    15721 ggtcgtgaga cggtcagagc ggcgcttaag catgccggtt tgctgtaaag tttagggaga
    15781 tttgatggct tactctgttc aaaagtcgcg cctggcaaag gttgcgggtg tttcgcttgt
    15841 tttattactc gctgcctgta gttctgactc acgctataag cgtcaggtca gtggtgatga
    15901 agcctacctg gaagcggcac cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc
    15961 ggtgacctcc ggtgattatg caatcccggt gaccaacggt agtggtgctg tcggtaaggc
    16021 gctggacatt cgtccaccag cccagccgct ggcactggtt tctggcgcgc gtacccagtt
    16081 cacgggcgat accgcttcat tgctggtgga aaatggtcgt ggcaatactc tgtggccgca
    16141 ggtggttagc gtgctgcagg cgaaaaacta caccatcacc caacgtgatg atgctggtca
    16201 gacactgacc accgattggg tacaatggaa ccgtctggac gaagacgagc agtatcgtgg
    16261 tcgttatcaa atctctgtta agccgcaggg ttatcagcag gcggttacgg ttaaactgtt
    16321 gaacctggaa caggcgggca aaccggttgc agacgcggct tccatgcagc gttacagcac
    16381 ggagatgatg aacgttattt ccgccggtct ggataaatct gccactgacg ccgcgaacgc
    16441 tgcgcaaaat cgtgcctcca ccactatgga cgtacaaagt gcagctgatg acaccggttt
    16501 accaatgctg gtcgtacgcg ggccgttcaa tgtggtttgg caacgtctgc cagcggcgct
    16561 ggaaaaagtg ggcatgaaag tgaccgacag cacccgttcg cagggcaaca tggccgtaac
    16621 ttataagccg ctgtctgaca gcgactggca ggaactgggc gcgagcgatc caggcctggc
    16681 atccggtgac tataaactgc aggttggcga tttagataac cgcagcagcc tacagttcat
    16741 cgatccgaaa ggtcatactc tgactcagag tcagaacgac gcgctggtag ctgtcttcca
    16801 ggctgcgttt agcaagtaaa aatacagggc tggaatcatc cggccctttt ttctgatatg
    16861 atacgcaaac gtgtgcgtcg gcaggaaaac gcgattttag cggtaattcg cacgaaattt
    16921 gtttgtcgga cgtagttcgg ataaggcgtt cacgccgcat ccgacaaaac atccggcaca
    16981 ccagacagca aaagatttta aaacgttaat tcacacccag gagtgataaa gatgcaaaag
    17041 caagctgagt tgtatcgtgg taaagcgaaa accgtataca gcacggaaaa cccggacctg
    17101 ttggtgctcg aattccgcaa tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt
    17161 gatcgcaaag gtatgttgaa caacaagttc aactacttca ttatgagcaa actggctgaa
    17221 gcgggtatcc cgactcaaat ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag
    17281 ctggatatgg tgccggttga gtgtgtcgtg cgtaaccgtg ctgcgggctc tctggtgaaa
    17341 cgtcttggaa tcgaagaagg tattgagctg aacccgccgc tgttcgatct gttcctgaaa
    17401 aacgatgcca tgcacgatcc gatggtcaac gaatcttact gcgaaacctt tggctgggtg
    17461 agcaaagaga acctggcgcg tatgaaagag ctgacctaca aagcgaacga cgtgctgaaa
    17521 aaactgttcg atgatgctgg tctgattctg gtcgacttca agctggagtt tggtctgtac
    17581 aaaggtgaag tggtactggg cgatgagttc tccccggacg gcagccgcct gtgggacaaa
    17641 gaaacactgg agaaaatgga caaagaccgt ttccgccaga gcctcggtgg cctgatcgaa
    17701 gcctatgaag ccgtcgcccg ccgcctgggt gtacagctgg actgattttt ctgttcatca
    17761 tcttgccgtg cagctggcac ggcaagacaa ccgctctcgg aatatatgca ttgaacgctc
    17821 attcctccgc catttcatcc cgctttcctg tggtaatctt cctctgaacc acctacgatc
    17881 atttctataa tgaatatatt gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg
    17941 acaatgtcga agacaggcgt aacagctccg gcggtccgtc aatgggcggt cccggttttc
    18001 gcctgccaag cggtaaaggc gggctgattt tactgatagt cgtgctggtt gcaggctact
    18061 atggtgttga tttaaccggg ttgatgaccg gacagccggt ttcccaacag caatcaacac
    18121 ggtcaattag cccaaatgaa gacgaagccg caaaattcac ctcggtgatt ctggcaacca
    18181 cggaagacac ctggggacag caattcgaga agatgggtaa aacctatcag caaccgaagc
    18241 tggtcatgta tcgtggaatg acgcgtaccg gctgcggggc gggccagtcc attatgggac
    18301 cgttctattg cccggcggat ggcacggttt atatcgatct ctccttctat gatgacatga
    18361 aagacaaact cggcgcggat ggcgattttg cccaggggta cgttatcgcc catgaagtcg
    18421 gtcatcatgt gcagaaattg ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg
    18481 cgacgcaggc ggaagtaaac cgcttatctg tgcgtatgga actccaggcc gactgttttg
    18541 ccggtgtctg ggggcataac atgcagcagc aaggcgatct ggaagaggcg ctgaacgcgg
    18601 cgcaggccat cggcgatgac cgtttacaac agcaaagtca ggggcgagta gtaccagaca
    18661 gtttcactca tggtacttct cagcaacgct acagctggtt taaacgtggt ttcgacagcg
    18721 gcgatccggc acaatgcaat acttttggta aaagcattta acttccgggg caaggatggc
    18781 tgaactgact gcgcttcaca cattaacagc gcaaatgaaa cgtgaaggga tccgccgctt
    18841 gctggtgttg agcggggaag agggttggtg ttttgatcat gcgcttaagt tgcgtgatgc
    18901 cttacctggc gactggctgt ggatttcgcc gcagccagat gctgaaaacc actgttctcc
    18961 ctcggcgcta caaactttac ttgggcgcga gttccggcat gcggtattcg acgcccgcca
    19021 cggctttgat gccgctgcct ttgcggcact tagcggaacg ttgaaagcgg gaagctggct
    19081 ggttttgtta ctccctgtat gggaagagtg ggaaaaccaa cctgatgccg actcgctgcg
    19141 ctggagtgat tgccctgacc ctattgcgac gccgcatttt gtccagcatc tcaaacgcgt
    19201 acttacggcg gataacgacg ctattctctg gcggcaaaac cagccgttct cgttggcgca
    19261 ttttaccccc cgtactgact ggcaccccgc gaccggcgca ccacaaccag aacaacagca
    19321 actcttacag cagctactga ccatgccgcc gggcgtggca gcggtaacgg ctgcgcgtgg
    19381 gcgcggtaag tccgcgctgg cagggcaact catttctcgt attgcgggca gtgcgattgt
    19441 caccgcgccc gcaaaagcgg caacggatgt actggcacaa tttgcgggcg agaagtttcg
    19501 ctttattgcg ccggatgcct tgttagccag cgatgagcaa gccgactggc tggtggtcga
    19561 tgaagccgca gccatacctg cgccgttgtt gcatcaactg gtatcgcgtt ttcctcgaac
    19621 gttgttaacc actacggtgc agggctacga aggtaccgga cgtggttttt tgctgaaatt
    19681 ttgcgctcgc tttccgcatt tacaccgttt tgaactgcaa cagccgatcc gctgggcaca
    19741 gggatgcccg ctggaaaaaa tggttagtga ggcactggtt tttaacgatg aaaacttcac
    19801 ccatacacca caaggcaata tcgtcatttc cgcatttgaa caaacgttat ggcgaagcga
    19861 tccagaaacg ccgttaaagg tttatcagtt attgtctggt gcgcactacc ggacctcgcc
    19921 gctggattta cgccggatga tggatgcacc agggcaacat tttttacagg cggctggcga
    19981 aaacgagatt gccggggcgt tgtggctggt ggatgagggc ggattatctc aagaactcag
    20041 tcaggcggta tgggcaggtt ttcgtcgccc gcggggtaat ctggtggccc agtcgctggc
    20101 ggcgcacggc agcaatccac tggcggcgac attgcgcgga cggcgggtca gccggattgc
    20161 agtccatccg gcgcgtcagc gcgaaggcgt tgggcaacag ctcattgcca gcgctttgca
    20221 atataggcct ggcctcgact atctttcggt gagttttggt tacaccgggg agttatggcg
    20281 tttctggcaa cgctgcggtt ttgtgctggt gcgaatgggt aatcatcgtg aagccagcag
    20341 cggttgctat acggcgatgg cactgttacc gatgagtgat gcgggtaaac agctggctga
    20401 acgtgagcat taccgtttac gtcgcgatgc gcaagctctc gcgcagtgga atggcgaaac
    20461 gcttcctgtt gatccactaa acaatgccgt cctttctgac gacgactggc ttgaactggc
    20521 cggttttgct ttcgctcatc gtccgctatt aacgtcgtta ggttgcttat tgcgtctgct
    20581 acaaaccagt gaactggcat taccggcgct gcgtgggcgt ttacagaaaa acaccagtga
    20641 cgcgcagtta tgtaccacac ttaaactttc aggccgcaag atgttactgg ttcgccagcg
    20701 ggaagaggcc gcgcaggcgc tgttcgcact taatgaggtt cgcactgagc gtctgcgcga
    20761 tcgcataacg caatggcaat tttttcactg actccttcaa ttttctccca tggtcattgt
    20821 gagtcagcgg cgtaaaatta atgacatcag ttaaggagtt cgccatgaaa catgaccatt
    20881 ttgttgttca aagcccggat aaacctgcgc aacagttgtt actgcttttc cacggtgtcg
    20941 gggataaccc ggtggcaatg ggggaaatag gcagctggtt tgcgccgcta tttcctgatg
    21001 cgctggtggt gagtgtcgga ggcgcggagc cgagcggtaa tccggcgggg cgtcagtggt
    21061 tttcggtgca gggtattacg gaagataatc gccaggcgcg tgtggatgcc atcatgccga
    21121 cgtttattga gatggtgcgc tactggcaga aacagagcgg agtaggggct aacgccacag
    21181 cgctaatcgg tttctctcaa ggcgcgatta tggcgctgga gagcattaaa gccgaaccgg
    21241 gccttgcttc acgcgtcatt gcttttaacg gacgctatgc gagcctgccg gaaactgcgt
    21301 ctacagccac gacaattcac ctgattcatg gcggtgaaga tccggttatc gatctggcgc
    21361 atgcggttgc ggcgcaggaa gcgttaatca gtgccggtgg tgacgtgacg ctggatatcg
    21421 tggaggattt aggccatgcg atcgacaatc gcagtatgca atttgccctg gatcacttgc
    21481 gttataccat tccgaaacat tatttcgacg aagcgctaag tggcggtaag cctggcgatg
    21541 atgatgtgat tgaaatgatg taaaagacag attacctggc ctgataagac gcgtgagcgt
    21601 cgcatcaggc atcgttcacc attgccggat gcggcgttaa cgccttatcc ggcctacggt
    21661 tgagcactca gcttacttct tcggttgatc ctttttcggc cagtcgtcgt catcgtccca
    21721 cttatcgttg aaatcacgat gcgggggaag ctccggcttg ttggcgagaa atttcttgtg
    21781 gtcgacgcgc ttgagatctt taatcacgtt cagcaggacg cctaccaaaa agacaatcac
    21841 cagaatccac caatatttag ccagccagtc catgcttatt tcctcttgca gaaccactca
    21901 tcaggcgacg agctgttcca tgatacgttg atacatacgg gcaagtagct gcaggtcggc
    21961 agcgttcaca cattcattaa ttttatgaat agtggcattg accggcccga gttccaccac
    22021 ctgcgccccc atgcgagcaa taaagcgccc gtcggacgtt ccgcctgtgg tcagtagctg
    22081 cggtttaatt tcattatagt gctcaaccgc gttaacgacc gcatccacca gtttaccgcg
    22141 cgcggtcaaa aatggctgcc cggaaagcca ccaatccacc gtatagcgca gttgatgttt
    22201 ttcaagcagg gcaagcacct gcgctttgat catctcatca gtcagttcgg tgctgaagcg
    22261 gaagttaaac tgcacaaaca gttcacccgg aataacgttg ttactgcccg ttcccgcctg
    22321 aatattggca atctgcatac tggtcgccgg gaagaattca ttgccctgat cccactcaat
    22381 agccactaat tcattaagga aaggtgctgc gcgatgtact ggattgtcag ccagatgtgg
    22441 gtaggccaca tgcccctgca cgccatgaat ggtaaggttg caggttaacg atccgcgacg
    22501 accatttttc accacatcac caaccacttc gatactcgac ggttcgccga ccagacagta
    22561 atcgagacgc tcattacgcg ccattaacgc ttcgacgact tttaccgtac cgttgtgggc
    22621 actggcttct tcatcagagg tgatcagaaa tgccagtcgt cccgtatggt tgggatgttg
    22681 tgcgacaaaa cgttctgctg ctaccaccat cgccgccagc gagcctttca tatctgccgc
    22741 accgcgcccg aataacatgc catcacgaat ggtgggttca aacggcggat tgatccaacg
    22801 atcggcgtcg ccaggcggca ccacgtcggt atgcccggca aaggccaatg tttcaccttg
    22861 cccacgccat gcccaaaaat tctgcgtatc ggcaaagtcc atgcgttcaa cggtaaaacc
    22921 gatcgcctgc aaacgatcaa tcaacaaagc ctggcatcct gcgtcatcag gactcaggga
    22981 agggcggcga ataagctgtt gtgtcagctc aataaccggg cacgacatag actacacctc
    23041 atggaaaaat tgctgataac tggaatcact gaaacccagc agcataggct taccgggcgc
    23101 gcagagcaat ggacgtttga taattgcagg catttcagtc attaatgccg ccgctgaggc
    23161 cgcatcggtg attttattgc gggtggtttc gtccagttta cgccaggttg taccacgggt
    23221 gttgagtaac gcctcccaac ctaattcgtt gataaaaccg ttcaataatt cgctgtccag
    23281 cccatcgacg cggtaatcat gaaaacgata gtcgatgtta ttggcttcca gccaacggcg
    23341 agccttttta atggtgtcac aatttttgat accgtaaagt gtaaccataa tgaatccttt
    23401 ttcgatttat gagaataaat actcatttaa gggtgaaaac attttgcctg caaatgatta
    23461 tcaggaaata taacattatc gaagtattca aattaacaac tattaaataa tattaaacat
    23521 ttttaacgta aacattaaga atattcatct tgttatctgt ctgtatttta acctgaatta
    23581 aatcagtatt agatgccgta tgaatatctc gaaatgttgc tgtgtatcac atacaaatca
    23641 tcaccgcagg accatagtat ttacataaga tttgtccccc cggaggtagt catcgcagca
    23701 atcgagtaaa ttagcttcac cacggtatat tgtttccgta gaatacccat aattataaga
    23761 gaggttgtta tgattgaacg tgaactgggg aactggaaag actttatcga agttatgctt
    23821 cgtaagtaat tcctgagaca aagtataaac agcaagaacc cgctgagcag gttcttaatc
    23881 ggaaagttga aaaaggcgac acattggcat gtcgcctttt ttattgctta ttccgggcgc
    23941 ggctttagcg ggaagcggcg gcgcaccagc acaaagaaca gcggcacgaa gtaaatagcc
    24001 agaatagtgg ctgaaatcat cccgcccatt acgccagtac ccaccgcatg ctgaccaccg
    24061 gaaccggcac ccgtgctggt tgccattggc aatacgccga agataaatgc cagcgaggtc
    24121 atcagaatcg ggcgtaaacg ctgacggcag gcgtggagcg tcgcttcaaa caggtcgtgg
    24181 cctttttggt tcatctcgtt agcaaactcg acgatcagga tggcgttttt cgccgataaa
    24241 ccaatgaccg ttaacaggcc cacctggaag taaacgtcgt tttccagccc gcgcatccag
    24301 gttgccagca gcgcgccgat tacccccagc ggcacgacca gcattaccga gaacggcacc
    24361 gaccagcttt catacaacgc agccagacac aggaatacca ccagcaagga aatggcgtac
    24421 agcgccggag cctgcgcgcc ggaaagtcgc tcctgatacg acatcgccgt ccactccaga
    24481 ccaaagccgt ttggcagctg cttcactaac gattccataa tatccatcgc cgtaccggta
    24541 ctgacccccg gtgcggcttc cccaacaatc tcaaccgcag aataaccgtt atagcgttcc
    24601 agacgcggcg agcctgtttc ccagcgtgag gtcgcgaaag cagagaaggg caccatgccg
    24661 ccatctttat ttcggacata ccagagattg atgtcatctg gcagcatgcg atacggcgca
    24721 gctgcctgca catagacttt cttcacgcga ccgcgatcca taaagtcatt cacatagctc
    24781 gaaccccagg cggtttgcag tgtgtcgtta atatcgtcga tagcaacgcc cagcgcctga
    24841 gctttacgct ggtcgatatc aatctgcaac tgcggactgt cgtcgaggcc gttatggcgc
    24901 acacgggtta gctccgggtt ttccgccgcc agcgccagca actggttacg tgctgccatc
    24961 agcgcatcgt gacctgctcc agcgtggtcc tgcaactcca tatcaaaacc cgcagaacta
    25021 ccaagaccgc taattgctgg cgggctgctg gcgataacgc gagcttcttt aatcttgtta
    25081 aacgcctttg ttgcacgctc gataatggca aacgaggtgc cggtcttact gtcgcgttcg
    25141 ctccagtctt tcaggcggat aaacattcgc gccacgtttt gcccgttacc cccagggcca
    25201 gaaccaacgg tggcaaacac cgacatgatg ttgtcttttt catgggtgaa gtaatatttc
    25261 tcgatttgct caacgacttt cagggtctgt tgttgggttg aaccgctggg caattgtacc
    25321 gaggtagtaa acatgccacg gtcttccagc ggtaagaacg acgtcggcaa acgcaggaac
    25381 aggaacacca tgccgccaag cagcaggaca taaatcacaa tccagcgcag gctgcggtgg
    25441 agaattttcg ccaccccttt ttcgtagcgt tcggcgttgc ggttaaacat ctggttaaac
    25501 caggcaaaaa agcctttttg cccgtgatgc tcaccttttt tcagtggctt aagtagtgtg
    25561 gcacacagag ccggagtgag gatcatcgct accagtactg acagcaccat cgccgcaaca
    25621 atggtaatag agaactggcg atagatggca ccggtggtgc cgccgaagaa ggccattggt
    25681 acaaataccg ccgaaagcac catcgcaatc ccgaccagtg ccccctggat ctgccccatc
    25741 gatttacgtg tggcttcgcg aggagtgagt ccttcctcac tcataatacg ttcgacgttt
    25801 tccaccacca cgattgcgtc atccaccagc agaccgatcg ccagtaccat cgcgaacatg
    25861 gttaaggtgt tgacgctgta accgaaggcg taaagtacgg agaaggttcc catcaacacc
    25921 accggcacgg cgatagtggg tatcagcgtg gcgcggaagt tttgcaggaa cagatacata
    25981 acgaggaaaa ccagggcgat agcttccagc agcgttttca ccacgtcttc aatggaggct
    26041 ttaacaaacg aggtggtttc atacgccacc ttgtattcca gtccgtgcgg gaagtactgc
    26101 gccagctcgt cgagacgatt gagcaccagc tccgctgtcg ccatttcgtt tgcgccggag
    26161 gccagtttta cccccagccc ggaggctggc ttaccattga agcggctaag ataatcgtat
    26221 ttctccgccc ccatttcgac ggtggcgaca tcgcccagcc ttacctctga gccgtcttga
    26281 ttcacccgca aggtgatatc gcggaactgt tctggtgttt gcagcagtga ctgggcgtta
    26341 atggtggcgt tgagcgcctg cttatcgacg gaaggtgtac caccaagttg cccaaccgca
    26401 atctgcgcgt tctgtgactc aatggcatca gtgacatctt tagccgtcat ctggaaactg
    26461 ttgagtttcg ccgggtccag ccagatacgc atggaatatt gcgaaccata ggcatcgata
    26521 tccccgacgc cattcacgcg gcttaacggg tcctgaatat tactggcaac ataatcggca
    26581 atatcctgtt tatccatcga accatcggta gagacgaaag caatggtcag aatattggta
    26641 tcgccggttt tacgcaccgt cacgccctga ttttgcaccg cctgcggtaa ctttcgcatg
    26701 gctgattgca gctggttttg tacttgctgc acggcttcgt ccggatcggt gcctgcttta
    26761 aaacttaaag tgacagatgc ctgaccgatg ccactgctct gagatgacat atacatgaga
    26821 ttatcgaggc cggtcatatt ttgctcgata acctgggtca cggtgttttc cagcgtctgg
    26881 gccgatgcgc cgggatagtt agcggtcact cgcacattcg gtggcgcgag atcggggtat
    26941 tgttcaacgg gcaatgaaaa aatcgccagg gtccctgtca gacacaacag gattgccagc
    27001 acccaggcaa aaatggggcg atcaataaag aaattcgcca ttaaaagagg acctcgtgtt
    27061 tctgcgtatc gttgtaggct tgacttgctt cactgtagcg gcaaggtacg agccaatcgt
    27121 ggagaaaaaa aggagttaat gtaaattatc atgccctttt atactgaacc ttaagtgcaa
    27181 gtattctttg gtcagtagga ggcattacat caactgactt tcctcaccct ccgcagagcg
    27241 aaagctaata ctcactaacg tgccgccgcc ggaaggttgt gaaaaagtca gcgtcccacc
    27301 cagccgttcc gcgcgttcgc gcatgatatt cagaccataa tgaccttcgg gttctttcgg
    27361 ttcaccgata ccaatcccgt tatcacggat ataaaccgtg tgattgccgt ccggcgcggt
    27421 gacgcaactg acggcgattt cgctggcgtt ggcgtgcttc atcgcattca gcaccgcttc
    27481 gcgaataatt tgcaacaaat gcacctgcat ttgcgcatcc agcgccaggg ttggcagacg
    27541 gcagtcgagg gtcagtttgg cgctggtttg attttgtaac gtatccagca tttccctcaa
    27601 cgcggagggg agatccgcct gctgcagcgt caggcggaaa gtagtcaaca gctcgcgtaa
    27661 ctgccgataa gcatcattca acgcctggga aaagtcggcc atgatacttt gtgcggtggc
    27721 gttatcttcc ggtatcgaac gcttcagtaa cgtcaactgg atacgtaagt aagaaagtac
    27781 ctgagccagc gagtcgtgca attcgcgggc gatggttgca cgttcttcca tcaacaataa
    27841 ctgctgaaaa tgcttttgcg cctgattaaa gtacaaaccg cgtcccagca tcgacgaaac
    27901 gctgttaagc agcggttctg atgatgaaac gtgactattt tgccagtgca gttcgccgta
    27961 aaccgtctct tgcattgtca ccggtaaaat ctgcatcggc aattccgggt ttggttgccc
    28021 ttcgctaatc cgccagtttt caccgacatt taactccaga tattcagccg cttcattgtc
    28081 gcgaacaatc tgcaaaatat ggcggaaaca atgcacatca atctggcttg tgttcagcgc
    28141 ctgcgaacat tgatacaaca cctccagacg acgcttggcc tcgtggagat cgcgggtctt
    28201 ttcttctact gacgcctcca gcgaaaggta caatttatgc agctcgctcg acatctggtt
    28261 aaaggttttt gcaagcagac caagctcatt cggcagactg gtatccagcg gcggcgagtc
    28321 gaactgcccg tgttcaatac gttgactggc ggtaaccagc tgattcagcg gggcaaccac
    28381 ctgatggcgt atacggcgca aagtaaaaaa gaccagcgtg aaaataccga taccgccagc
    28441 cagggaaatc gccaccacca gcagcatttt gcgttcagcg tagtgctgta aagccagcac
    28501 gaacaggtct atctgattaa cataattatt aatattggcc tgataccacg gcaaatcgcc
    28561 cttgctgagc cgattattca tctccagcca gttggcattc agatgcgcat agcgagtttt
    28621 tactgcttct ggcacatacc agacgttgag attggttaat accgatgaat gcagtgcctg
    28681 ctgaaacaac tggcgatgtg cattgagttg tggactgcca ctttgcaagt cgtagcccag
    28741 gcggtaactc tgcatacgca gcgatccggc aatattgata gcctcagcgt cgcgcaaact
    28801 gctcgccaga gttagcagag cgatacccgt ggaaagaatc gacagcagca caatgtaaaa
    28861 aaaggcccgg gccagactgg ccgagacggg tcgtttaaca atcacacgcg tcttctccac
    28921 aaaaaatcat taatgttcca gttcgctaaa acgcaattta gtcagtgatg ggattatttg
    28981 atctgccaca gtttatagcg aaatagttac ttattctggg cacatgaaca ttgccgattc
    29041 ccctgtaagt cgggtaataa caacactcat ataaagaata aggtttttac aaccaaaaaa
    29101 gaaggtcgtt atgaatcgtt ttattatggc caacagtcag caatgtctgg gttgtcatgc
    29161 ttgtgaaatc gcctgtgtca tggctcacaa tgatgagcaa catgtcctga gccaacacca
    29221 ttttcatccc cgaattacgg ttatcaaaca tcagcagcaa cgtagtgcag tgacctgtca
    29281 ccattgtgaa gatgcgccct gcgcccgtag ctgccctaat ggcgcaatca gccacgttga
    29341 tgacagcatt caggtcaatc agcaaaagtg tattggctgt aaatcctgcg tggtggcctg
    29401 tccgtttggt acgatgcaaa tcgtcctgac acccgtcgcg gcaggaaaag taaaagccac
    29461 ggcgcataaa tgcgaccttt gtgcagggcg cgaaaacggt cctgcctgtg tcgagaattg
    29521 cccggcggac gcgctgcaac tggtcactga cgtcgcactc tccggcatgg cgaaatcccg
    29581 ccgcttgcgc accgcacgtc aggagcatca accgtggcat gccagtaccg ctgcacaaga
    29641 aatgccggta atgagtaaag tcgaacaaat gcaggcaacg cccgcgcgtg gcgagccgga
    29701 taaactggcg attgaagcgc gcaaaaccgg ttttgatgaa atttatctgc cattccgtgc
    29761 cgatcaggca caacgggaag cctcgcgctg ccttaaatgc ggcgagcaca gcgtttgtga
    29821 atggacctgc ccgctgcata accatattcc gcagtggatt gaactggtga aagccggaaa
    29881 catcgacgcc gccgtcgagc tttctcacct gaccaacacc ctgccggaaa ttaccggacg
    29941 cgtttgtccg caggaccgtt tgtgtgaagg tgcctgtact attcgcgatg agcacggcgc
    30001 ggtaactatc ggcaacattg aacgctacat ttcagatcag gcgttggcga aaggttggcg
    30061 tcctgactta agccatgtca ccaaagtgga caagcgggtg gcgattatcg gtgcgggtcc
    30121 ggcagggctg gcctgtgcgg atgttctgac ccgcaatggc gtggcggtga cggtgtacga
    30181 tcgccatcca gaaattggtg gcttgctcac tttcggcatt ccttctttca aactggataa
    30241 atccctgctg gcacgccgtc gggaaatctt cagcgcgatg gggattcact tcgaactcaa
    30301 ttgtgaagtg ggtaaagatg tctctttgga ttcgcttttg gaacaatacg acgcggtctt
    30361 cgttggcgta ggcacttacc gttccatgaa agcgggttta cccaatgaag atgcgccggg
    30421 cgtttatgac gcgctgccgt tcctcatcgc caacactaaa caggtgatgg gactcgaaga
    30481 gctaccggaa gagccgttta tcaataccgc cggactcaac gtcgtggtac tgggcggcgg
    30541 cgacaccgcg atggactgtg tgcgtaccgc gctgcgccac ggcgcgagta acgtcacctg
    30601 cgcttatcgt cgtgatgaag ctaacatgcc aggctcgaag aaagaggtga agaacgcccg
    30661 cgaagagggg gccaacttcg aatttaacgt ccagccggtg gcgcttgagc tgaatgagca
    30721 aggtcacgtt tgcggaattc gtttcctgcg cacgcgtctt ggcgagccgg atgcccaggg
    30781 gcgccggcga cttgtaccga ttgagggcag tgaatttgtc atgccagccg atgcggtgat
    30841 tatggtgttt ggcttcaatc cacacgggat gccgtggctg gagtcgcacg gtgtaacggt
    30901 agacaaatgg ggccgcatca tcgcggatgt ggaaagccag taccgttacc agaccaccaa
    30961 tccgaaaatc ttcgccggtg gcgacgccgt gcgtggtgcg gatctggtgg ttaccgcaat
    31021 ggcagaagga cgtcatgcgg cacaggggat tattgactgg ctgggggtaa aatcagtcaa
    31081 atctcactga tagcctgcgc agacaaaccc gacttcacag cgtaatataa ttgttcattt
    31141 cgcgctgtgg agtcggtatg acgcaacaaa tcaccctcat taaagacaaa attctctccg
    31201 ataactattt caccctgcac aacattactt acgatctcac gcgcaaagat ggcgaagtta
    31261 tccgccataa acgtgaagtt tacgatcgcg gtaatggtgc gacgatcctc ctgtacaacg
    31321 cgaagaaaaa gaccgtggtt ctgattcgtc agttccgtgt cgctacctgg gttaatggca
    31381 atgaaagcgg gcagctgatt gaaacctgcg ccgggctgct ggataacgac gaaccggaag
    31441 tgtgcattcg taaagaagcg attgaagaga cgggctatga agtgggcgaa gtgcgcaaat
    31501 tatttgaact gtatatgtcg cctggcggtg tgactgagct aatccacttt tttatcgccg
    31561 aatacagtga caatcagcgc gctaacgccg gtggcggtgt tgaagatgaa gatattgaag
    31621 tgcttgagct gccgttcagc caggcgctgg agatgatcaa aaccggcgag atacgtgacg
    31681 gtaagacggt gttattgctt aactatttgc aaacgtcaca tttaatggac tgaaaaataa
    31741 caataatatt tcgttgttta ttattggctc aatccgacaa atctgattga gccgcgctac
    31801 ctgcgcaacg aagatacgac ttgtgctgtt tgtttgaact tctggggtcg taccgtccat
    31861 gcgctatcgc attttccttc tctttttttt cgctttgttg ccgacgtctt tggtgtgggc
    31921 ggcactagcg caacgggcgt tttccgactg gcaggtcacc tgtaataacc aaaacttctg
    31981 cgtggcgcgc aatacgggcg atcataatgg actggtgatg accctgagcc gcagcgccgg
    32041 ggcgcatacc gatgccgttt tacgtattga gcgcggcggg ttgaagtcgc cggatgcgtc
    32101 tgaaggagag atagcgccac ggctgctgtt agatggtgag ccgttggcgt taagtggtga
    32161 caagtggcgg atttcaccat ggttattagt aaccgatgat acggcaacca tcaccgcgtt
    32221 tttgcagatg attcaggaag ggaaggcgat caccttacgc gacggcaatc agaccatttc
    32281 tctgagtggc ttaaaagcgg cgttgttgtt tattgatgct cagcaaaagc gcgttggcag
    32341 tgaaaccgcg tggatcaaga aaggggacga accgccgctc agcgtaccgc ccgcgcctgc
    32401 gctgaaagag gtcgcggtgg ttaacccaac gccgacgcca ctctcactcg aagaacgcaa
    32461 cgatttgctg gattatggaa actggcggat gaatggtctg cgctgctcgc ttgatccgtt
    32521 acgtcgtgag gtgaatgtca ctgcgctgac tgatgataaa gcgctgatga tgatcagctg
    32581 tgaggcaggg gcctataaca ccattgattt ggcatggatt gtgtcgcgta aaaagccgct
    32641 agcctcgcgc ccggttcggt tgcgtttgcc gttcaacaac gggcaggaga cgaatgaact
    32701 ggaactgatg aacgcaacat ttgatgagaa atcgcgtgaa ctggtgacct tagcgaaagg
    32761 gcgcggatta agcgattgtg gcattcaggc gcgctggcgc tttgacggtc agcgttttcg
    32821 cctggtgcgt tacgccgcag aacccacctg cgataactgg catgggccag acgcctggcc
    32881 cacgttgtgg atcacccgtt aaacaaaatg gcggggacat gtaggccgga taagacgctg
    32941 taggccggat aagacgcgtc agcgtcgcat ccggcaatca gcatccggcg atcactatca
    33001 ggcacctttc actcccagca ccttatgcgc ttttgccaca atattctcgg cggtaaagcc
    33061 aaagaacggg aacagcttat ccgccggagc agattcccca taacccgtca tcccgacaat
    33121 tgcccctttc agaccaacat acttgtacca gtaatcggca atacctgctt ccaccgccac
    33181 gcgagccgca acgttagaag gcaacaccga ctcccgatat tcctcatcct gggcgtcgaa
    33241 aatatcggtc gagggcaggg aaaccacgcg aacattgcga ccttctcctg ctaatttctc
    33301 cgcagcttgc agggtgattt ccatctctga accggtggca atcagaataa tatctggctt
    33361 accgccgctg tctttcagca cataaccgcc acgagtaatc tctttaacct gatccggcgt
    33421 acgttccacc tgggccagat tctgccttga gaggatcagt gccgtcggtc cgttgtggcg
    33481 ctcaaccgcc agcttccagc ccaccgccgc ttccacctga tcgcacggtc gccaggtgct
    33541 gaaatttggc gttaagcgca ggctggccag ttgctcaaca gcctggtgcg tcggaccatc
    33601 ttcgcccagg ccaattgagt cgtgggtata aaccataatc tgccgcgctt tcatcagtgc
    33661 cgccatccgc gcggcgttac gggcgtattc aacaaacatc aggaacgtcg cggtatacgg
    33721 cacaaagccg ccgtggtgcg cgatgccgtt ggcgatagcg gtcatgccaa attcacgcac
    33781 cccgtagtga atgtagttgc ccgccggatc ttccttcagc gaaacagaac ctttccagat
    33841 ggtcaggttg ctgggagcca gatccgccga accgccgagc agctccggta gcaaggggcc
    33901 ataagcgtta agcgtatttt gcgaagcctt acgggtggcg attttcgccg gattcgcctg
    33961 caactcattg atatacttct gagtcgtttt ctcccagtcc ttcggtaaac caccgctcat
    34021 ccgacgggta aactcttctg ccagttgcgg atgagccttt ttataggcgg caaacttctc
    34081 attccacctc tgctgcgctt tttcgccttt ttcgcgggca tcccaggcgt gataaatatc
    34141 tttagggatc tcaaatggcg gatggtgcca gcccagtttt tgccgtgcca gcgccacttc
    34201 ttcttccccc agcggtgcgc cgtgcgcctc ttccttacct gctttattcg gcgatccaaa
    34261 gccaatcacc gtacgacaga taatcagcga cggcttatct ttcacgcttt gcgcttcaag
    34321 gatcgcttcc ttcaccgcct gcggatcgtg accgtcgatt tcatggatca catgccagtg
    34381 ataggcttca aaacgttttg ccgtatcgtc ggtaaaccag ccttctgttt caccgtcgat
    34441 ggaaataccg ttgtgatcgt aaaaaccaat cagcttgccc agtcccagcg tgcctgccag
    34501 cgaacagact tcgtgggaaa taccttccat caggcagccg tcgcccataa acacataggt
    34561 gaagtgatcg acgatctcat ggtctggctg gttaaactgc gccgccagtg tacgctctgc
    34621 tatcgccagc ccgacggcgt tcgccaaacc ttgtccaagt ggaccggtgg tggtttcaac
    34681 gcctggcgta taaccaatct ccgggtggcc tggggttttc gaatgcagtt gacgaaagtt
    34741 cttcagctct tccagcggca ggtcgtaacc ggtcagatgc agcaaactgt agagcagcat
    34801 cgacgcgtga ccgttggaaa gaataaagcg gtcgcgatca taccaggttg ggtcggtagg
    34861 gttatgttta agaaaatcgt tccacagcac ttcggcaata tcagccatgc ccatcggcgc
    34921 gccgggatga ccagaattgg ctttttgtac cgcatccata ctgagtgcgc gaatcgcatt
    34981 ggcaaggtct tttcgggaca tataacactc cgtggctggt ttatagtttg gcggcaagaa
    35041 gatcttccag tttgcgttga tcaacggcga acagacgaat gccttcagac agtttttcta
    35101 ccgccatcgc atactgattg tgctcccaac ggaactccgc ttcgctcatg ggagctgggc
    35161 gtgggaacgt ctgagaaggt gggattaatt tacgtaccac tggcgaaact ttttcctgca
    35221 gctccttcag caaattcggt gcgatagtca gtcgatcgca gccggttaag gcgaggattt
    35281 gttcggtgcg acggaagctc gcgcccatca caatggtttc atagtggtgt tgcttatagt
    35341 agtcgtagat attgcgcacc gatttaacgc ccggatcttc ttccaccaca tacgggtcca
    35401 tcggcttgcg tgcctgatac cagtcataaa tacgcccgac aaacggcgaa atcagaaaaa
    35461 cgcctgcttc cgcacaggcc cgtgcctgtg caaaagaaaa cagcagcgtc aggttgcagt
    35521 taataccttc tttttccagc tcttctgccg cgcgaattcc ttcccaggtc gaagccagct
    35581 tgatcagaat gcgtgatttc tcaacgcctt gttgctgata caagtccacc agatggcgcg
    35641 ccttctcaat actcttttct ttatcaaaag agaggcgtgc atcaacttct gttgacacgc
    35701 gaccgggtac gattttgagg atttcagcac cgaaattgac cgccagtttg tcacacgctg
    35761 cgaccacctg ttgttcctgg gtcttgccat tttttttacc ccgggcgata gcatcgtcta
    35821 ttaaatgctc atattgtgat aatccggcag ccttgagtaa cagcgaagga ttggtggtgg
    35881 catcctgggg atgataatgg cgaatggact caatatcgcc gctgtctgcc acgacagtgg
    35941 tgaactgttt gatgccgtct aactcgttca taggaaatac tccttgaaaa gtaaagtgtt
    36001 agatgagtgc gttaattcac acttctgaga aatttcgcta aacgcatcaa aaaagcatag
    36061 cagacaggca tggtattgct ggattaagca ggtaacatca gtgttatagg attattacca
    36121 aaacattata ttaatggtga gagtttggac ttgctcaaag tctgtagact ccggcaaggt
    36181 aataatgtgc gccacgttgt gggcaggggg tgttgcccac acactttatt tgtgaacgtt
    36241 acgtgaaagg aacaaccaaa tggatgacca gttaaaacaa agtgcacttg atttccatga
    36301 atttccagtt ccagggaaaa tccaggtttc tccaaccaag cctctggcaa cgcagcgcga
    36361 tctggcgctg gcctactcac ccggcgttgc cgcaccttgt cttgaaatcg aaaaagaccc
    36421 gttaaaagcc tacaaatata ccgcccgcgg taacctggtg gcggtgatct ctaacggtac
    36481 ggcggtgctg gggttaggca acattggcgc gctggctggc aaaccggtga tggaaggcaa
    36541 gggcgttctg tttaagaaat tcgccgggat tgatgtattt gacattgaag ttgatgaact
    36601 cgacccggac aaatttattg aagttgtcgc tgcgctcgaa ccgaccttcg gcggaatcaa
    36661 cctcgaagac attaaagcgc cagaatgttt ctatattgaa cagaaactgc gcgagcggat
    36721 gaatattccg gtattccacg acgatcagca cggcacggca atcatcagca ctgccgccat
    36781 cctcaacggc ttgcgcgtgg tggagaaaaa catctccgac gtgcggatgg tggtttccgg
    36841 cgcgggtgcc gcagcaatcg cctgtatgaa tctgctggta gcgctgggtc tgcaaaaaca
    36901 taacatcgtg gtttgcgatt caaaaggcgt tatctatcaa ggccgtgagc caaacatggc
    36961 ggaaaccaaa gccgcatatg cggtggtgga tgacggcaaa cgtaccctcg atgatgtgat
    37021 tgaaggtgcg gatattttcc tgggctgttc cggcccgaaa gtgctgaccc aggaaatggt
    37081 gaagaaaatg gctcgggcac caatgattct ggcgctggcg aatccggaac cggaaattct
    37141 gccgccgctg gcgaaagaag tgcgtccgga tgccatcatc tgcaccggtc gttctgacta
    37201 tccaaaccag gtcaacaacg tcctttgctt cccgttcatc ttccgtggcg cgctggacgt
    37261 tggcgcaacc gccatcaacg aagagatgaa actggcggcg gtacgcgcga ttgcagaact
    37321 ggcccatgcg gaacagagcg aagtggtggc ttcagcgtat ggcgatcagg atctgagctt
    37381 tggtccggaa tacatcattc caaaaccgtt tgatccgcgc ttgatcgtta agatcgctcc
    37441 tgcggtcgcg aaagcggcga tggagtcggg cgtggcgacg cgtccgattg ctgatttcga
    37501 cgtctacatc gacaagctga ctgagttcgt ttacaaaacc aacctgttta tgaagccgat
    37561 tttctcccag gctcgcaaag cgccgaagcg cgtagttctg ccggaagggg aagaggcgcg
    37621 cgttctgcat gccactcagg aactggtaac gctgggactg gcgaaaccga tccttatcgg
    37681 tcgtccgaac gtgatcgaaa tgcgcattca gaaactgggc ttgcagatca aagcgggcgt
    37741 tgattttgag atcgtcaata acgaatccga tccgcgcttt aaagagtact ggaccgaata
    37801 cttccagatc atgaagcgtc gcggcgtcac tcaggaacag gcgcagcggg cgctgatcag
    37861 taacccgaca gtgatcggcg cgatcatggt tcagcgtggc gaagccgatg caatgatttg
    37921 cggtacggtg ggtgattatc atgaacattt tagcgtggtg aaaaatgtct ttggttatcg
    37981 cgatggcgtt cacaccgcag gtgcaatgaa cgcgctgctg ctgccgagtg gtaacacctt
    38041 tattgccgat acctacgtca atgacgaacc agacgcagaa gagctggcgg agatcacctt
    38101 gatggcggca gaaactgtcc gtcgtttcgg tattgaaccg cgcgttgctc tgttgtcgca
    38161 ctccaacttt ggttcttctg actgcccgtc gtcgagcaaa atgcgtcagg cgctggaact
    38221 ggtcagggaa cgtgcgccag aactgatgat tgatggtgaa atgcacggcg atgcggcgct
    38281 ggtggaagcg attcgcaacg accgtatgcc ggacagccct ttgaaaggtt ctgccaatat
    38341 tctggtgatg ccgaacatgg aagctgcccg cattagttac aacttactgc gcgtttccag
    38401 ctcggaaggt gtgactgtcg gtccggtgct gatgggcgtg gcgaaaccgg ttcacgtgtt
    38461 aacgccgatc gcatcggtgc gtcgtatcgt caacatggtg gcgctggccg tggttgaagc
    38521 gcaaacccaa ccgctgtaat tcttttttaa ctctcacgct taccctgaat attcagggta
    38581 agcggtttgg ctgcaatata ttagtaaagc ttattactga gtttgcgaat aataaaaaaa
    38641 gcagtctata taatatctcg atattatttt atttatattc atgcgttgca tatgaaagtt
    38701 tatgcaccac agcgaatatc tctccattct tagtgatcta cctcaccttt taaacgcgct
    38761 tgccgaattt tgttatttac tctgacgaaa aattgtcacg atacacgaaa gtttttcaca
    38821 ggcggcgact catggataaa gaacgcatca ttcaggaatt tgtgcccggc aaacaggtca
    38881 cactggcgca tctcatcgcg catcccggtg aggaactggc gaagaaaatc ggcgttccgg
    38941 atgcgggcgt aattggcatt atgacgctaa ctcccggcga aacggcgatg atcgtcggtg
    39001 atttagcgct gaaagccgct gatgtgcata tcggttttct cgaccgcttc agcggtgcat
    39061 tggtgattta tggctcggtg ggcgcagtag aagaagcgtt atcacaaacc gtcagcggtt
    39121 taggtcgctt attaaattat acgctttgtg aaatgaccaa aagttaatac gaggcattca
    39181 tgaaacgaat tgcgtttgtg ggtagcgtcg gggcaggaaa aacaacgtta tttaatgcgt
    39241 tgcaggggaa ttataccctc gccagaaaaa cacaggccgt ggaatttaat gataagggcg
    39301 atattgatac gccgggtgaa tattttagcc atccccgctg gtatcacgcc ttaattacca
    39361 cgctacagga tgtagacatg ctgatctatg tccatggcgc aaatgacccg gaaagtcggt
    39421 tgcctgccgg gttgctggat attggcgtca gcaaacggca aatcgccgtc atcagtaaaa
    39481 cggacatgcc agatgctgac gtcgccgcaa cgcgaaagtt attgcttgag acgggtttcg
    39541 aagagccgat tttcgagcta aacagccacg acccgcaaag cgtgcagcaa ctggtggatt
    39601 atctggcatc tctcaccaaa caggaggaag caggtgaaaa aactcatcac agcgaatgat
    39661 attcgtgaag cacacgcgcg cggcgaacag gcaatgtccg tggttctgcg cgccagcatt
    39721 attaccccgg aagcccgtaa agtggcggat ctactgggtt ttaccatcac cgaatgtgat
    39781 gaatcgatcc cggtaacggc gtccgtgccc gccagcgtgc cagccgacaa aaccgaaagc
    39841 cagcgcatcc gcgaaaccat cattgcccag ctgccggaag gccagtttac cgaaagcctg
    39901 gtcgcacagc tgatggaaaa agtgatgaag gaaaaacagt cgctggagca gggcgcaatg
    39961 cagccgagct tcaaatcggt gaccggcaaa ggcggcatca aagtgattga tggcagcagc
    40021 gtcaagtttg gtcgttttga tggcgcacaa ccgcattgcg tgggcttgac tgatcttgtg
    40081 accggagacg acggcagcag catggccgcc ggttttatgc agtgggagaa cgcattcttc
    40141 ccgtggaccc tgaactacga cgaaatcgac atggtgctgg aaggggagct ccatgttcgc
    40201 cacgaaggcg aaaccatgat cgccaaagca ggcgacgtga tgtttatccc gaaaggctcc
    40261 agcatcgaat ttggtacgac atccagcgtg aaattcctgt atgtcgcctg gccggctaac
    40321 tggcaatccc tatgaaagat ttcatcaccg aagcatggct aagagcgaac catacgctca
    40381 gcgaaggcgc agagatccat ctccctgcgg acagtcgcct gacgccctct gcccgggagt
    40441 tgctggaaag ccgccatctg cgcatcaagt ttattgacga gcagggccgc ctgtttgttg
    40501 acgatgaaca gcagcagccg cagcccgttc atgggctgac cagtagcgat gaacatccgc
    40561 aggcgtgctg cgaactgtgc cgccagccgg tggcaaaaaa gccggatacg ctgactcacc
    40621 tgtcggcgga aaaaatggtc gccaaaagcg atccgcgtct gggttttcgc gcggttctcg
    40681 acagcaccat tgcgctggca gtgtggctgc aaattgaact ggcggaaccg tggcagccgt
    40741 ggctggcgga tatccgttcg cgtcttggca acattatgcg cgccgatgcg ctgggggaac
    40801 cgctgggctg ccaggcgatt gtcgggcttt ctgacgaaga tctgcatcga ctttctcacc
    40861 agccgctgcg ctatctcgac cacgatcatc tggtgccgga agccagccac gggcgcgatg
    40921 cggcgttgct caatctgcta cgtaccaaag tgcgggaaac cgaaaccgta gcggcgcagg
    40981 tgtttatcac ccgcagcttt gaagtattac gcccggacat cttgcaggcg ctgaaccgcc
    41041 tttcgagcac ggtctacgta atgatgatcc tgagcgtaac gaagccgccg ctaacggtga
    41101 agcagatcca acaacgactg ggggaaacgc aatgattatt gaacgttgtc gtgaactggc
    41161 gttgcgagcg cccgccagag tggtttttcc ggatgcgtta gatcaacgtg tgctgaaagc
    41221 tgcgcaatat ttacatcaac aaggtctggc aacgcccatt ctggtcgcta atccgtttga
    41281 acttcgtcag tttgcgctca gtcacggcgt ggcgatggac gggctacagg tgattgatcc
    41341 gcatggcaac ctcgcaatgc gggaagaatt tgctcatcgc tggctggccc gcgcgggcga
    41401 aaaaacgccg ccggatgcgc tggaaaaact taccgatccg ctgatgttcg ccgcagcaat
    41461 ggtcagcgcc ggtaaagcgg atgtctgtat cgcgggcaac ctctcttcca cggcgaatgt
    41521 gctgcgtgcc ggattacgta ttatcggctt gcagccaggc tgtaaaacgc tctcatccat
    41581 tttcctgatg ctgccacagt acagcggtcc ggcgttgggc tttgccgatt gcagcgtggt
    41641 gccacagccg acggcggcgc aactggcgga tatcgcgctt gccagtgccg aaacctggcg
    41701 cgccctcacc ggagaagagc cgcgcgtggc gatgctgtcg ttttccagta acggtagcgc
    41761 ccgtcacccc tgcgttgcca acgtccagca ggcgacagaa atcgtccgtg agcgcgcacc
    41821 aaagctggtg gttgatggcg agttgcagtt tgacgccgcc ttcgtgccgg aagtggcggc
    41881 gcaaaaagcg cctgccagcc cgctacaggg caaagccaat gtgatggttt ttccgtcgct
    41941 ggaagccgga aatattggct acaaaatcgc acaacgactc ggcggatatc gtgccgtcgg
    42001 gccattgata caaggacttg ccgcgccgat gcacgatctc tctcgtggtt gtagcgtgca
    42061 ggaaattatc gagctggcgc tggtggcagc tgtgccgcgt cagacagaag tgaaccgcga
    42121 aagcagttta caaacactgg ttgaatgaac ggtcccgttc tggaccctga taaagaggaa
    42181 agcacaatgg aagcattagg aatgatcgaa acccggggcc tggttgcact gattgaggct
    42241 tctgacgcga tggttaaagc tgcgcgcgtc aagctggttg gtgttaagca gattggcggc
    42301 ggcctgtgta ctgccatggt tcgtggtgat gttgcagcct gtaaagccgc gaccgatgca
    42361 ggtgccgcag cggcacagcg tatcggcgag ctggtatctg tacacgttat cccgcgtccg
    42421 cacggcgacc tggaagaagt cttcccgatc ggcctgaaag gcgatagcag caacctgtaa
    42481 tcaccctgtg tgacgtcgat tcgggcgtca cgctctctcc tttttcattt taccttctgc
    42541 ggagggtagg gggagctttt acctgaaata cgccacggag gcgggtatga aactggcagt
    42601 cgtcactgga caaattgttt gtaccgtacg ccatcacgga ctggcgcatg acaaattgct
    42661 gatggtggaa atgattgatc cacaaggtaa tcctgacggg caatgcgccg tcgccatcga
    42721 caatattggc gcgggaaccg gggagtgggt gttactggtg agtggcagtt ccgcccgcca
    42781 ggcgcataaa agcgaaacgt caccggtcga tctgtgcgtg attggcattg tcgatgaggt
    42841 ggtgtctggc ggtcaggtaa ttttccacaa ataaggcaga acatcatgaa tcaacaggat
    42901 attgaacagg tggtgaaagc ggtactgctg aaaatgcaaa gcagtgacac gccgcccgcc
    42961 gccgttcatg agatgggcgt tttcgcgtcc ctggatgacg ccgttgcggc agccaaagtc
    43021 gcccagcaag ggttaaaaag cgtggcaatg cgccagttag ccattgctgc cattcgtgag
    43081 gcaggcgaaa aacacgccag agatttagcg gaacttgccg tcagtgaaac cggcatgggc
    43141 cgcgttgaag ataaatttgc caaaaacgtc gctcaggcgc gcggcacgcc aggcgttgag
    43201 tgtctctctc cgcaggtgct gaccggcgac aacggcctga ctttaattga aaacgcgccc
    43261 tggggcgtgg tggcttcggt aactccgtcc actaacccgg cggcaaccgt aattaataac
    43321 gccatcagcc tgattgccgc gggcaacagc gtcatttttg ccccgcatcc ggcggcgaaa
    43381 aaagtctccc agcgggcgat cacgctgctc aaccaggcga ttgttgccgc aggcgggccg
    43441 gaaaacttac tggttactgt ggcaaatccg gatatcgaaa ccgcgcagcg tttgttcaag
    43501 tttccgggca tcggccttct ggtggtgacc ggcggcgaag cggtagtaga agcggcgcgt
    43561 aaacacacca ataaacgtct gattgccgca ggcgctggca acccgccggt agtggtggat
    43621 gaaaccgccg acctcgcccg tgccgctcag tccatcgtca aaggcgcttc tttcgataac
    43681 aacatcattt gtgccgacga aaaggtactg attgttgttg atagcgtagc cgatgaactg
    43741 atgcgtctga tggaaggcca gcacgcggtg aaactgaccg cagaacaggc gcagcagctg
    43801 caaccggtgt tgctgaaaaa tatcgacgag cgcggaaaag gcaccgtcag ccgtgactgg
    43861 gttggtcgcg atgcggcgaa aatcgcggcg gcaatcggcc tgaacgtgcc gcaagaaacg
    43921 cgtttgctgt ttgtggaaac cactgcagaa catccgtttg ccgtgaccga actgatgatg
    43981 ccggtgctgc ccgtcgtgcg cgtcgccaac gtggcggatg ccattgcgct ggcggtgaaa
    44041 ctggaaggcg gttgccacca cacggcggca atgcactcgc gcaacatcga aaacatgaac
    44101 cagatggcga atgctattga taccagcatt ttcgttaaga acggaccgcg cattgccggg
    44161 ctggggctgg gcggggaagg ctggaccacc atgaccatca ccacgccaac cggtgaaggg
    44221 gtaactagcg tgcgtacgtt tgtccgtctg cgtcgctgtg tattagtcga tgcgtttcgc
    44281 attgtttaag gagtaacaga tggcgcacga cgaacaatgg ctcaccccac gtctgcaaac
    44341 ggcggcaacg ctgtgtaacc agacgcccgc cgcgacggaa tctccgctgt ggctgggggt
    44401 ggatctgggc acctgcgatg tggtgtcgat ggttgtcgac cgcgacggtc agtcggtggc
    44461 ggtatgcctc gactgggccg atgtggtccg cgacggcatc gtctgggatt tcttcggcgc
    44521 tgtcaccatt gttcgtcgcc atctcgacac gctcgagcag caattcggtc gccgttttag
    44581 ccatgcggcg acttcatttc ctccaggtac ggacccgcgt atttccatca acgtgctgga
    44641 gtctgctgga ctggaagtga gccatgtgct ggatgagcca accgcagttg cggatctgct
    44701 gcaactggac aacgccggtg tagtggatat cggcggcggc actaccggca ttgccatcgt
    44761 gaaaaagggc aaggtgacgt actcggcgga tgaagcaacc ggcggacatc acatctctct
    44821 gactctcgcc ggaaatcgcc gtatttcgct ggaagaggcg gagcagtaca agcgcggtca
    44881 cggtgaagag atttggccta cggtgaaacc ggtgtacgaa aaaatggtgg acattgtcgc
    44941 ccggcatatt gaagggcagg gcattactga tttatggctg gcgggcggct cttgtatgca
    45001 accgggcgtg gcggaactgt ttcgcaaaca attcccggcg ttacaggtgc atttaccgca
    45061 gcacagtttg tttatgaccc cgctggcgat cgccagtagt gggcgagaga aagcggaggg
    45121 gctatatgca aaatgaattg cagaccgcgc tctttcaggc gttcgatacc ctgaatctgc
    45181 aacgggtaaa aacatttagc gttccaccgg tgacgctttg cggtccgggc tcggtgagca
    45241 gttgcggaca gcaagcgcaa acgcgtgggc tgaaacatct gttcgtgatg gcagacagct
    45301 ttttgcatca ggcagggatg accgccgggc tgacgcgcag cctggctgtt aaaggcatcg
    45361 ccatgacgct ctggccatgt ccggtgggcg aaccgtgcat caccgacgtg tgtgcagccg
    45421 tggcgcagtt gcgtgagtca ggctgtgatg gggtgatcgc atttggcggc ggctcggtgc
    45481 tggatgcggc gaaagccgtg gcgttgctgg tgacgaaccc ggatagcacg ctggcagaga
    45541 tgtcagaaac cagcgttctg caaccgcgct tgccgctgat tgccattcca acgaccgccg
    45601 gaaccggctc tgaaaccacc aatgtaacgg tgattatcga cgcggtgagc gggcgcaagc
    45661 aggtgttagc ccatgcctcg ctgatgccgg atgtggcgat tctcgacgcc gcgttgaccg
    45721 aaggtgtgcc gtcgcatgtc acggcgatga ccggcattga tgcgttaacc catgccattg
    45781 aagcatacag cgccctgaac gctacaccgt ttaccgacag tctggcgatt ggtgccattg
    45841 cgatgattgg caaatcgctg ccgaaagcgg tgggctacgg tcacgacctt gccgcgcgcg
    45901 agagcatgtt gctggcttca tgtatggcgg gaatggcgtt ttccagtgcg ggtcttgggt
    45961 tgtgccacgc gatggcgcat cagccgggcg cggcgctgca tattccacac ggtctggcga
    46021 acgccatgtt gctgccaacg gtgatggaat ttaaccggat ggtttgtcgt gaacgcttta
    46081 gtcagattgg tcgggcactg cgaactaaaa aatccgacga tcgtgacgct attaacgcgg
    46141 taagtgagct gattgcggaa gttgggattg gtaaacgact gggcgatgtt ggtgcgacat
    46201 ctgcgcatta cggcgcatgg gcgcaggccg cgctggaaga tatttgtctg cgcagtaacc
    46261 cgcgtaccgc cagcctggaa cagattgtcg gactgtacgc ggcggcgcaa taaatgccgg
    46321 atgcgacgct tgccgcgtct tatccagcct acgggattgc acatgtaggg cggataaggc
    46381 gttcacgccg catccgccag tggcgcggtg caattgccgg atgcgatgct tgccgcgtct
    46441 tatccggcct acgggattgc acatgtaggg cggataaggc gttcacgccg catccgccag
    46501 tggcgcggtg caattgccgg atgcgacgct tgccgcgtct gatccggcct tcgggattgc
    46561 acatgtaggg cggataaggc gtttacgccg catccgccaa taaataacaa ctaacaggga
    46621 gtaaaggcga tgggaattaa cgaaatcatc atgtacatca tgatgttctt tatgctgata
    46681 gctgccgtag acaggatcct gtcgcagttc ggcggttctg ctcgtttcct cggtaagttc
    46741 ggtaaaagta tcgaaggatc aggcggtcag ttcgaagaag gctttatggc aatgggcgct
    46801 ctgggtctgg cgatggtcgg tatgaccgcg ctggcaccgg tactggctca cgtactcggg
    46861 ccggtaatta tcccggttta cgaaatgctc ggcgcaaacc cgtcaatgtt cgccggaacg
    46921 ctgctggcgt gcgatatggg cggcttcttc ctcgccaaag agctggcggg cggcgacgta
    46981 gcagcgtggc tatactctgg gttaattctt gggtcgatga tggggccaac gattgtgttt
    47041 tccattccgg tggcgctcgg cattatcgaa ccttctgacc gtcgttatct ggcgctcggc
    47101 gtgctggcgt gcattgtgac cattccgatt ggctgtattg ctggtggtct ggttgctatg
    47161 tactccggtg tgcagatcaa cggtcagccg gtggaattca ctttcgccct gatcctgatg
    47221 aacatgatcc cggtgatcat tgttgcgatt ctggtggcgc tggggctgaa attcatcccg
    47281 gaaaaaatga tcaacggctt ccagatcttc gccaaattcc tcgttgcatt gatcaccctc
    47341 ggtcttgctg ccgcggtagt taagtttctg cttggctggg aactgatccc cggtctggac
    47401 cctatcttta tggcccctgg cgataaaccc ggtgaagtga tgcgcgccat tgaagttatc
    47461 ggttctatct cctgcgttct gttaggggcg tatccaatgg tgctgctgct gacccgctgg
    47521 tttgaaaaac cgctgatgag cgtcggtaaa gtgctgaata tgaacaacat cgcggcagcc
    47581 ggcatggtgg caacgcttgc caacaacatc ccgatgttcg gcatgatgaa gcagatggat
    47641 acccgcggca aagtcatcaa ctgcgccttc gccgtttccg ctgctttcgc cctgggcgac
    47701 cacttaggct tcgccgctgc caacatgaac gccatgatct tcccgatgat tgtcggcaag
    47761 ttgatcggcg gcgtaacggc gattggcgtg gcgatgatgc tggtgccaaa agaagacgcg
    47821 accacggcta aaaccgaagc ggaggcacaa tcgtgaacac tcgccagcta ttgagcgtcg
    47881 gtatcgatat cggcaccacc accacccagg tgattttctc ccacctggag ctggttaacc
    47941 gtgcggcggt gtcgcaggtg ccgcgctacg aattcattaa acgcgaaatt agctggcaaa
    48001 gcccggtgtt ctttacccct gtcgataaac agggtggttt aaaagaagcg gaactgaaaa
    48061 ccttaatcct cgagcaatat caggctgcgg gcattgcgcc ggaaagcgtt gattctggtg
    48121 ccatcatcat caccggtgaa agcgcgaaaa cccgcaatgc tcgtccggcg gtgatggtgc
    48181 tctcccagtc gctgggcgat tttgtcgttg ccagcgccgg gccgcacctc gaatccgtca
    48241 ttgccggtca cggtgccggg gcacaaaccc tttctgaaca acggctgtgt cgggtactga
    48301 atattgacat cggcggtggc actgcgaact acgccctgtt cgatgccgga aaaatcagcg
    48361 gcactgcctg cctcaacgtc ggtggtcgcc tgctggaaac cgacagccag gggcgcgtgg
    48421 tttacgctca taaaccgggg cagatgattg tggatgagtg cttcggcgca ggcaccgacg
    48481 cccgttcgct gaccggcgcg cagctggtgc aggttacccg gcggatggca gaactgattg
    48541 tcgaagtgat tgacggaacg ctctcgccgc tcgcgcaggc attgatgcaa accggtttgc
    48601 tgcccgcagg tgttacgccc gaaatcatta cgctttctgg cggcgtgggc gaatgttatc
    48661 gccaccagcc cgccgacccg ttctgttttg ccgatattgg cccgctgctg gcaacggcgc
    48721 tgcatgacca tccgcgcctg cgtgagatga acgtacagtt tccggcgcaa accgtgcgcg
    48781 ccacggtgat tggcgcgggc gcgcataccc tttcgctctc tggcagcaca atctggcttg
    48841 agggcgtaca actgccgctg cgcaatttgc cggtggcgat cccgattgat gaaacggatc
    48901 tggtgagtgc ctggcaacag gcgctgcttc agctggatct tgatcccaaa actgacgcgt
    48961 acgtgctggc gcttcccgcc tcgctgcctg tgcgttacgc cgcggtactg acggtcatca
    49021 acgcgctggt cgatttcgtc gcgcgttttc caaatccgca tcccctgctg gtggtggccg
    49081 ggcaggactt tggtaaagct ctgggcatgt tgttgcgccc acagctacaa caactcccgt
    49141 tggcagtcat tgacgaagtg attgtccgcg cgggggacta tatcgacatt ggtacgcctc
    49201 tttttggcgg atcggttgtg ccggtgacgg tgaaatcact cgcatttcct tcctgaggga
    49261 acgacttatg aaactaaaga ccacattgtt cggcaatgta tatcagttta aggatgtaaa
    49321 agaggtgctg gctaaagcca acgaactgcg ttcgggggat gtgctggcgg gcgttgcagc
    49381 ggcaagctca caggagcgcg tggcggcaaa gcaggtgttg tcggaaatga ccgtagcgga
    49441 catccgcaat aatccggtga ttgcctatga agatgactgc gtgacgcggc tgattcagga
    49501 cgatgttaac gaaacggcct acaaccagat taaaaacagg agcatcagcg aactgcgtga
    49561 gtatgtgctg agcgatgaaa ccagcgtgga cgacattgcc tttacccgca aagggctgac
    49621 ctcggaagtg gtcgcggcgg tagcgaagat ttgctccaac gcggacctga tctacggcgc
    49681 gaagaaaatg ccggtaatca aaaaggccaa taccaccatc ggtattccgg gcaccttcag
    49741 cgcccgtttg cagccaaatg acacccgtga cgacgtgcaa agtatcgctg cacaaatcta
    49801 cgaagggctt tccttcgggg tgggcgatgc ggtgatcggc gttaacccgg taactgacga
    49861 cgtggaaaac ttaagccgcg tgttggacac catttacggc gtgatcgaca aattcaacat
    49921 cccaactcag ggctgcgtac tggcgcacgt caccacccag atcgaagcga ttcgtcgcgg
    49981 cgcgcctggc ggactgattt tccagagtat ctgtggcagc gaaaaagggc tgaaagagtt
    50041 tggcgtggaa ctggcgatgc tcgacgaagc gcgcgcagtg ggtgctgagt tcaaccgtat
    50101 cgccggggaa aactgcctct acttcgaaac cgggcaaggt tcggcgctgt ctgctggcgc
    50161 taacttcggc gcagactagg tgacgatgga agcacgtaac tatgggctgg cgcgtcatta
    50221 cgatccgttt atcgtcaaca ccgtggtcgg ctttattggg ccggagtatc tctacaacga
    50281 ccgccagatt atccgtgctg gcttagaaga tcactttatg ggcaagctga gcggcatctc
    50341 tatgggctgt gactgctgtt acaccaacca cgctgatgct gaccagaacc tcaacgaaaa
    50401 cctgatgatc ctgctcgcca ccgcaggctg caactacatc atggggatgc cgctgggcga
    50461 tgacatcatg ctcaactatc agaccaccgc attccacgac actgccactg tgcgtcagtt
    50521 actcaacctg cgcccgtcac cggagtttga acgctggctg gaaagcatgg gcattatggc
    50581 aaacggtcgc ctgaccaaac gggcgggcga tccgtcactg ttcttctgat gacgcggaga
    50641 taacacatca tggatcaaaa acagattgaa gaaattgtac gcagcgtgat ggcgtcaatg
    50701 ggacaagcgg cccccgcgcc gtcagaagca aagtgcgcca ccactaactg tgcggcaccg
    50761 gtgacctcgg aaagctgcgc gctggattta ggttctgcgg aagcaaaagc gtggattggt
    50821 gttgaaaacc cgcatcgcgc agacgtatta acagaactgc gccgcagcac cgtggcccgc
    50881 gtttgtaccg gtcgtaccgg tccgcgtcca cgtacgcagg cgctgctgcg tttcctggcc
    50941 gatcactctc gttcgaaaga taccgtactg aaagaagtgc cggaagagtg ggtgaaagcg
    51001 cagggcttgc tggaagtacg ctccgaaatt agcgacaaaa atctctacct gactcgcccg
    51061 gatatgggcc gccgcctgtg tgcagaagct gttgaagcgc tgaaagcaca gtgtgttgct
    51121 aatccggacg tacaggtcgt tatttctgat ggcctgtcaa ctgatgccat caccgtgaac
    51181 tacgaagaga tcctgccgcc gctgatggcg ggcctgaaac aggccgggct gaaagtcggc
    51241 acgccattct ttgttcgtta tggtcgcgtg aagattgaag atcagatcgg tgagatcctc
    51301 ggcgcgaaag tggtgatcct gctggtgggg gaacgtccgg ggttgggtca gtcagaaagc
    51361 ctctcctgtt acgccgttta ctcgccgcgt atggcgacca ccgttgaggc cgatcgcacc
    51421 tgcatctcta acattcacca gggcggcacg ccgccggttg aagccgcagc cgtcattgtg
    51481 gatttggcga aacgtatgct ggagcagaaa gcatccggca tcaacatgac ccgataagga
    51541 ggcatcatgc cagctttaga tttgattcga ccgtcggtaa ccgctatgcg ggtgattgcc
    51601 tccgttaacg ctgaatttgc gcgtgaacta aaattgccgc cgcatattcg tagcctcggg
    51661 ctgatttctg ctgattccga tgacgtcacc tacattgcgg ctgacgaagc gaccaagcag
    51721 gcgatggtcg aagtggtata tggccgctcg ctgtatgcgg gagcagcaca cggaccgtca
    51781 ccgactgccg gtgaagtgct gattatgctt ggtgggccaa acccggcgga agtgcgtgct
    51841 ggtctggatg cgatggttgc gcatattgaa aatggcgctg ctttccagtg ggctaatgac
    51901 gcagaaaaca ccgcattcct ggcacatgta gtttcgcgta ccggttctta tctttcatca
    51961 accgccggga tcacgcttgg cgatccgatg gcgtatctgg tggcaccgcc gctggaagcg
    52021 acctacggca ttgatgcagc gttgaaatct gccgacgtac agctggtgac ctatgtcccg
    52081 ccaccgtctg aaaccaacta ctcggcagca tttttaaccg gtagccaggc cgcgtgtaaa
    52141 gcagcctgta acgcctttac cgatgcagtg ctggaaatcg cgcgtaatcc aatccagcgt
    52201 gcgtaacgga ggttgccgat gatcaatgca ctgggattgc tggaagtgga cggcatggtc
    52261 gccgcgatag atgcagcgga tgccatgctc aaagcagcta acgttcgtct gctcagtcac
    52321 gaagtgcttg accctggtcg cttaacgctg gtggtggaag gcgatctggc ggcgtgtcgt
    52381 gcggcgctgg acgcaggttg tgctgccgcg atgcgtaccg ggcgtgtcat cagccgcaag
    52441 gagatcggtc ggccagacga tgacacccag tggctggtta ctggctttaa ccgccagccg
    52501 aagcaacccg taaaggaacc cgacgcgcca gttatcgtcg cggaatctgc tgacgagttg
    52561 ttggcgctgt taacatcagt acgtcaggga atgacggcag gagaagtggc tgcccacttt
    52621 ggctggccgc tggaaaaagc cagaaatgcg ctcgaacagc tcttttctgc cgggacgtta
    52681 cgtaaacgca gtagtcgtta tcgtctcaag ccccattaac ctgtcggagg tgccgggtgt
    52741 cctacaacac ccggcattaa catcatgaaa aagacccgta cagccaattt gcaccatctt
    52801 tatcatgaac ccttacccga aaacctgaag ctcacgccga aggtcgaagt ggataatgtt
    52861 catcaacgac agacaacgga tgtctatgaa catgctttga cgattaccgc ctggcagcag
    52921 atttacgatc agctgcatcc gggcaagttt catggtgaat ttacggaaat tctgctcgat
    52981 gatattcagg tttttcgtga atacaccggt ctggcgctgc gtcagtcgtg cctggtctgg
    53041 ccgaactcgt tctggtttgg cattccggcg acgcgcggtg agcagggatt tatcggttcg
    53101 caatgtctgg gaagcgcaga aatagcgacg cgccccggtg gaaccgagtt tgaattaagt
    53161 acgccggacg attatacgat cctgggcgta gtgctgtctg aagatgtcat cacccgtcag
    53221 gctaactttt tgcacaatcc ggatcgggta ttacatatgc tgcgtagcca gtcggcgctg
    53281 gaagtgaaag agcagcataa agccgcgctg tggggctttg tccaacaggc gctggcgacg
    53341 ttttgcgaga acccggaaaa tctccatcag cctgcagtgc gaaaagtgct gggggataat
    53401 ttgctaatgg cgatgggggc gatgctggaa gacgcgcaac caatggtgac ggcggaaagc
    53461 atcagtcatc agagttaccg tagattactt tcccgcgccc gtgaatatgt gctggaaaat
    53521 atgtctgagc tggtgacggt gctggacttg tgtaatcaac tgcatgtcag tcgccgcacg
    53581 ctacaaaacg cgtttcacgc tattttaggc attggcccaa acgcgtggct gaaacgcatt
    53641 cgcctgaacg ccgtacgccg cgaactgata agcccgtggt cgcaaagcac aacggtaaaa
    53701 gacgccgcca tgcagtgggg attctggcat ctggggcaat ttgccacgga ttaccagcag
    53761 ctgtttgccg agaagccgtc gttgacgttg catcagcgga tgcgggaatg ggggtgaggg
    53821 agttacaccc aatccctgac cttaataaac tcacttaacg ccgcttctgg gctgccatct
    53881 tttggctgat aatcatactc ccagcgtacc agtggcggca ttgacatcag gatcgactcg
    53941 gtgcgcccgc cagtttgcag gccaaacaga gtgccgcgat cccagaccag attgaactcg
    54001 acataacgac cgcgacggta gagctgaaaa ttgcgctcgc gctcgccgta ggccatcgct
    54061 ttacgtcgct ctacaattgg taaataagcg tcggtgtagc ctttgcctac cgcctgcata
    54121 aaggcaaaac agtggtcgaa atctggcgtg ttcagatcat caaagaacag cccgccaata
    54181 ccgcgctgtt cgttgcgatg tttgaggtag aagtaatcgt cgcaccactt tttgtaacgg
    54241 ggataaacgt cttcaccaaa tggcaggcac aggtcacggg cggtgcggtg ccagtgaatg
    54301 gcgtcttctt caaaaccata gaaaggggtt aaatcgaagc cgccgccaaa ccaccagacg
    54361 ggctctgcac ccggtttttc ggcaataaaa aaacgcacat tcgcgtggct ggtgggaaca
    54421 tacgggttat gcggatgcac taccagtgaa acgcccatcg cctcgaaact gcgcccggca
    54481 agttccgggc gatgagcggt ggcggaagca ggcatcgcct cgccgtggac gtgagaaaag
    54541 ttgacgcctg cctgttcgaa aacaccacca ttacgcaaca cccgactacg cccgccgccg
    54601 ccagcttcgc gctgccaact atcttcgaca aattctgcgc catcgacggc ggtcagctgc
    54661 tgacaaatcg tatcctgaag gttgagcaga aactgtttaa cctggtgtgc gtcgggtttc
    54721 ataacttatc gctttttcga atgtgctttc tggttgtcga accagtggaa ataactgatc
    54781 acgccttcag caatcgctgt ggcgattttc tgacgaaacg ccgccgtgcc taacagccgc
    54841 tcttcttccg ggttggtgat aaacgaggtt tccaccagca ccgaaggaac cgacggcgat
    54901 ttcaacacca caaacgcggc ttgttcggtg ttgcggctgt gcagtttatg caccggctta
    54961 atctttttca gaatatgcga gccaagcgtc aggctgtttt taatagtatc ggtttgcacc
    55021 agatcaaaca gcacttgctg caataggtga tccttgtcag tcgccttttt accggcaact
    55081 tcatcggcgc ggttttcgcg ttcagacagg tatttcgcca ttgcgctact tgccccacgg
    55141 ttagagaggg caaataccga agcaccggca gctttcgggt tggtaaagcc atcggcatga
    55201 attgacataa acagatctgc gccatgttta tgggcgattt caacgcgatc gtaaagtggg
    55261 ataaacgtat cgccagaacg cgttaaacgc gcatcaatcc catgattacg caaaatggaa
    55321 cggacgtttt tagcaatcgc cagcaccaca tgtttttctt tcgaaccgtt gcgtccgatc
    55381 gctccggtat caattccgcc gtggcctgga tcgagaacaa cgacacgttt gccgccagat
    55441 tttttggctt tcggcttgct gtgtccgttg ctggttttta aaggttcgtc tttggcgatg
    55501 gcttgcgaca ttcctgacaa cgtcagggca gccaaaccgg ctttcagcac ctggcggcgc
    55561 gaagtgagtg tttttagtgg tttaaaagtg ctcatacggc ctgagttgta ataataaagt
    55621 tccagatgtt atatcgtatc gcgtaatccg ttacgacggt ttgatgttga gaattgtttt
    55681 acttttcatt tcaatagttg acaactcccc attatgccac tttttcaacc gattttcgtc
    55741 agatattggc taattcgacc gttcacgcca taatcagcct tttaacccgc aaaaaggtaa
    55801 cgaataccat ggagatacgc gtatttcgcc aggaagattt cgaagaggtc atcacccttt
    55861 gggagcgttg cgacttgctg cgtccgtgga acgatccgga aatggacatc gagcgtaaga
    55921 tgaaccatga cgtcagtttg tttttggtcg ctgaggtaaa cggtgaagtg gtcggaacgg
    55981 tgatgggcgg ttatgacggg catcgcgggt ctgcttatta tcttggcgtg catccagagt
    56041 ttcgtgggcg tgggattgcc aatgcgttgc ttaatcggct ggagaaaaag ctgattgctc
    56101 gtggctgccc gaaaattcag atcaatgtgc cggaagataa cgacatggtg ctcggaatgt
    56161 atgaacgcct gggatatgaa cacgccgacg tgctgagtct gggtaagcgt ttgattgaag
    56221 atgaagagta ctgagttcca tcctgtccat tatgatgcgc acggacgcct gcgtttaccc
    56281 ttgctcttct ggcttgtgct attgcttcag gcgcgaacct gggtgctgtt tgtcattgcc
    56341 ggtgcgtcgc gcgagcaggg tactgcgctg ctaaatctgt tttatcccga tcacgataat
    56401 ttctggctgg ggctgattcc aggcattccg gcggtgctgg cgtttttgtt gagcggtcgg
    56461 cgggctacat ttcctcgcac ttggcatgtg ctctattttc tgttgctgtt ggcgcaggtg
    56521 gttttacttt gctggcaacc gtggctgtgg ctgaacggtg aatccgttag cggtatcggt
    56581 ctggcgctgg tagtggcgga tattgtggcg ttaatctggc tgctgaccaa tcgacgttta
    56641 cgcgcttgtt ttaatgaaga aaaagaataa cggcactttt tggtgaattt gcactccaag
    56701 caacgttatt gaataaccaa aggaagtgac atgaaatcgc tgcgtttaat gttatgcgct
    56761 atgccgttga tgctgaccgg ctgttccacg atgtcgtcag ttaactggtc ggccgctaac
    56821 ccgtggaact ggtttggatc gtccaccaaa gtgagcgagc agggcgtggg tgaattaacg
    56881 gcgtccacac cactgcaaga acaagccatt gctgatgcgc ttgatggcga ttatcgcctg
    56941 cgcagcggaa tgaaaaccgc gaacggcaac gtggtgcgct tttttgaagt gatgaaaggc
    57001 gacaacgtgg cgatggtgat taacggcgat caaggcacga tcagtcgcat tgacgtgctg
    57061 gatagcgata ttcctgctga caccggtgtt aaaatcggta caccgtttag cgacctttac
    57121 agcaaagcat ttggcaattg ccaaaaagct gatggtgatg ataatcgtgc cgtcgaatgt
    57181 aaagccgaag gcagtcaaca tattagctac cagttcagcg gggaatggcg tggtcctgaa
    57241 gggttaatgc cttcggacga taccctgaaa aactggaaag tcagtaaaat tatctggcgg
    57301 cgttaatttg cgtctgaaca aaccgccgcc gcgaaaaaac acgtaaaata gcgctcaaca
    57361 atgccacgga ttgcgtggca ttcttatttt caggaggaac aatgtctcag gttcagagtg
    57421 gcattttgcc agaacattgc cgcgcggcga tttggatcga agccaacgtg aaaggggaag
    57481 ttgacgccct gcgtgcggcc agtaaaacat ttgccgacaa actggcaact tttgaagcga
    57541 aattcccgga cgcgcatctt ggtgcggtgg ttgcctttgg taacaacacc tggcgcgctc
    57601 tgagcggcgg cgttggggca gaagagctga aagattttcc gggctacggt aaaggccttg
    57661 cgccgaccac ccagttcgat gtgttgatcc acattctttc tctgcgtcac gacgtaaact
    57721 tctctgtcgc ccaggcggcg atggaagcct ttggtgactg cattgaagtg aaagaagaga
    57781 tccacggctt ccgttgggtg gaagagcgtg acctgagcgg ctttgttgac ggtacagaaa
    57841 acccggcggg tgaagagacg cgtcgcgaag tggcggttat caaagacggc gtggatgccg
    57901 gcggcagcta tgtgtttgtg cagcgttggg agcacaacct gaagcagctc aaccggatga
    57961 gcgttcacga tcaggagatg atgatcgggc gcaccaaaga ggccaacgaa gagattgacg
    58021 gcgacgaacg tccggaaacc tctcacctca cccgcgttga tctgaaagaa gatggcaaag
    58081 ggctgaagat tgttcgccag agcctgccgt acggcaccgc cagtggcact cacggtctgt
    58141 acttctgcgc ctactgcgcg cgtctgcata acattgagca gcaactgctg agcatgtttg
    58201 gcgataccga tggtaagcgt gatgcgatgt tgcgtttcac caaaccggta accggcggct
    58261 attacttcgc gccgtcgctg gacaagttaa tggcgttgta agcgtttctg attcgatgat
    58321 taaaggccag ccagtaacaa aactgactgg cctttttatt acttttgctt caacgccgca
    58381 tacacctgat caactaccca accataagtt gcaatcggta actgaccgct ttcgaacata
    58441 ttgggatttt tttgtggatc ggcaactggc gaatggggct tgttgcttaa catcacaatc
    58501 gccatatgat tgaccggatc gataacggtt actgtcccgg tccagccagt gtgaccgtag
    58561 gtttgcgggc ttgccagcgt gccaaacgtc ggcgtcatgg tggcattacc attcacgcgc
    58621 cagccgaggc caaaagtggc atcttcctta gagctggtgg tgaacatctt caccgtttcc
    58681 gcactgaaca gctgcacatc gccatagccc ccgccgttca gcatcgtttg cattaacacc
    58741 gcaatatcgc cggtattgga aaacaaacct gcgtgcccgg aaacgccgcc catcgaataa
    58801 aaggcttttt catcgtgcac ctgaccccag agagtggagg tgcggatatt cgggaaatgg
    58861 atcacgccat cgcgggtatt gccgttcaat tccgtggcgg caatttgttg cggtttaaag
    58921 cctttcagca gcgggttaaa taccgtatgt gtcaggccga gcgggcgata aatcgactct
    58981 tcaacatagc ggtcaagcgg ctgaccggta accgactcga cgataaatcc aagcagcata
    59041 taatcgacat cgctgtagat atgtttgctg ccgggctgat attccagcgg cgtgcgcttg
    59101 atcatctcca gcgtctggcc tttatcctgg gaatataacg cgcccgcgac cgctttattc
    59161 gggtattgcg gatctgccgg gaaaccgccg ctgtgatgca gcaggtcaga aatccgcagg
    59221 gtgtttttgc ctttaatggt gtcatttgga ctatcggcaa accccggaat atatttcgca
    59281 atccgatcgt caggatgcag tttgccttcg ctcatcagct tttgcagggc gaagttcgtg
    59341 gcgtacattt tggtgtttga ggccaggtca tacagcgtcc cggtggtggc tttgacaggc
    59401 tgttccatca acacgctgcc atcgtacttt ttcgccgctc cccaggcttt gcgatacaca
    59461 atctgattat ctttaatgat cagcaggttt acgccgggat aaccgacatc aatttgctgg
    59521 ctaatccagc gatccatctg gttaagccgt tcgacgttaa accctgcttt ctctggcgag
    59581 ctttctgtca gaacagggta ttttgcggcg ctgacgctac aactaacagc cagcagagaa
    59641 agatagagca ttgtccgttt catgatatca gtccagattg acgttacggc agccaaagag
    59701 cgtggtgaaa ataaacccgc aaacccatgc caccagaatc ccgccagcat aaaccgccat
    59761 ggccggaagg atgccttgtg cggaagtcat cagcggcagc gctaccagac cagacggccc
    59821 aaaggcgctg tttaagccca tcggcagacc ccatcaggct atcaggccga taaacaaacc
    59881 gcccgccgcg ccgcctaaac aggcggtaac aaacggcttc atgcggggca gggtgacacc
    59941 gtaaatcagc ggttcaccaa cgcccagcag gccgggaata atcgccccgc gtacctgact
    60001 gcgtagcgca ctgtgcggtt gcgcccgcca gtagagtgcc agcgccgcgc ccacctggcc
    60061 cgcgcctgcc attgaaagga tgggaaataa gctgttgaat ccctagctgt ccattaacgc
    60121 gaggtaaaca ggaataaagc cctgatgcac gccaaatacc acggcgatca ggaacagccc
    60181 ggctaaaacc gcacaaccga acggattact gttcaggtgc ataaacagcc atgacatacc
    60241 ttcgaataac cagccaccca gcggcataat gatcaggtac gcaagcgtag cggtgatcag
    60301 cagggtgatt aacgaggtca gcagcatgtc gagatcgtcc gacataaagc ggcgcaccat
    60361 gccttcaatg cgggcgcagg cccacgcggc aatcagcacg ccgataatat tgccgcgcgg
    60421 atcgatgggc agaccaaaga aatcgtgaaa accggcgtag tcaccggtgg ttgcagcggg
    60481 gttataaccg agcaaaaaga gtgcggcaat aattgcgcca ttgacgcccg tgccgccaaa
    60541 tgcctgggcg gcgttatagc ccaccagaat caccaggaaa gtgaacaaac ctttgctgaa
    60601 caccttcata aaattcagcg cgtcgggtag cgtgccctga gcatctgccg gaacgtgcat
    60661 caccgtggca attaacgtcg ctatccccag cagcagaccg gcggcaataa aaccggggat
    60721 cagcggcgtg aagatggtgg cgaatttggc gagaaattgt tgcacgccgg aggtttgttt
    60781 ggcttttaac tgacgtttgt tctgggcggc gatttctgcg gcatcctgta ccggtgcctc
    60841 gcccagcagc tcgctcatcg cttttgcggc acgatgcgct ttaccaggcc caaaaacgac
    60901 ctgcacctgg tcgctggtca aaatgacgcc cttcacgcct tccagcgttt tgatattggg
    60961 atcaaccagt gaactgtcat gtacacccag acgcaggcgc gtcatacagt taccacaact
    61021 ggcgatattt cccagtccgc cgacacgggt aagaatggtg ttcagaagtt cactgctgat
    61081 ctctttggcc atgggttatt ccttgtctaa aacctgacga ataaagccgc cgtgttgatc
    61141 caggcgtttt ttagcttctg cggcatcgag atttttcagc accatcacaa tggccgtttt
    61201 acagttgcgc tcgcaggcaa ttaacgccgc ttccgcttgt tcggcattac atccggtggc
    61261 gtttttaacg atattgacct ggcgcacatg cagtttttcg ttggtggcga ccacatcgac
    61321 catcaggttg ccgaacactt tgccggattt aatcatcagc ccggtggaaa gcatattgag
    61381 caccagtttc tgcgctgtgc ctgctttcat tcgcgaagaa ccagtgacca cttcggcacc
    61441 tacaatcggt gtaatggcaa actcggcggt ggtggaaacg gcactccccg gattacagga
    61501 aattcccact gtgcggcaac cgagctggcg ggcgtattcc agtccggcaa tcacatacgg
    61561 cgtacgaccg ctggcggcaa taccgaccac cacatcctgt gccgttaaat ttatattttt
    61621 cagatcgtta atgccgcctt ctcggctatc ttccgtgcct tccaccgcgt gctgaatggc
    61681 atattcgccg ccagcaatca aaccaaccac cagacccggt ttcacgccgt aggtgggcgg
    61741 acattcgctg gcatccagaa tccccagacg accggatgtt cccgcaccga gataaatcag
    61801 acgaccgccg ccgcttacct gggcgtggat aacatcgatc gccgcggcga tatccggcag
    61861 gacgcgctca acggcaagcg gtacggtttt atcttcatcg ttgataatcc ggcacatttc
    61921 cagcgtcgat acgcggtcaa tttcagccga ggcggtgttc gagccttcag taatcatctt
    61981 ttcaagttgc atcggtggtg accttactat ttacgatcaa ataattgcag atgtatggaa
    62041 tcatatatcc cgaattgaca gacttaaagg aatcattgag tctacacttg cagagtgtga
    62101 agagcgtcat aagaacttaa gcggaatttt tgctgccagc ccgggagagt ggaagatgtt
    62161 gtacctgacg aaaatacgca atgcggaaag tgaatttacc gagaacgagc aaaaaattgc
    62221 tgactttctg cgggccaacg tgagtgagct gaaatccgtc tcttcgcgca agatggcgaa
    62281 acagctcggc atcagtcagt cgagcattgt gaaatttgcc cagaagctcg gcgcgcaagg
    62341 atttactgaa ttgcgtatgg cgttaattgg cgaatacagt gccagccggg agaaaacgaa
    62401 tgccacggcg ctgcacctgc acagttcgat taccagtgat gattcgctgg aagtgattgc
    62461 ccgcaaactg aatcgcgaaa aagagctggc gctggagcaa acctgtgcgt tgttcgacta
    62521 cgctcggctg caaaaaatca ttgaggtcat cagtaaagca ccgtttatcc agataacggg
    62581 cctgggcggg tcggcgctgg tggggcgcga tctgtcgttc aaactgatga aaattggcta
    62641 tcgcgtggcc tatgaagccg acacacacgt acaagccacc gtttcccagg cgttgaaaaa
    62701 aggcgatgta caaatcgcta tctcttacag cggcagtaaa aaagagattg tcttgtgcgc
    62761 agaagcggca cgaaagcagg gggcaacggt gattgccatt acttctctgg ctgactctcc
    62821 gctgcggcgg ctggcgcatt tcacgctcga taccgtttcc ggtgaaaccg aatggcgtag
    62881 ctcgtcgatg tctacccgca cggcgcaaaa ttctgttacc gatttactgt ttgttggtct
    62941 tgtgcaactc aatgatgtgg agtcattgaa aatgattcag cgcagcagtg agctaacaca
    63001 acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg
    63061 atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt
    63121 attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc
    63181 gcattgcagg gaattggcga aggaattgcc agaacttttg cgcgtcatgg cgcaaaccta
    63241 atcttgctgg atatctcccc tgagatcgaa aagctggcgg acgaactgtg tggtcgtggt
    63301 catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc
    63361 aaacgcgcga aggaaaaaga agggcgcatt gatatcctgg tgaataacgc aggcgtttgt
    63421 cgtctgggca gtttcctcga tatgagcgat gacgatcgcg atttccatat tgacatcaat
    63481 attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc ccgcaaagat
    63541 ggtcgcattg tgatgatgtc ttcagtcact ggtgatatgg tggccgatcc tggcgaaacg
    63601 gcgtacgcct taacgaaagc ggcgattgtt ggcctgacaa aatcgctggc ggtggagtac
    63661 gcgcagtctg gtattcgcgt taacgccatt tgcccgggat acgtgcgcac accaatggcg
    63721 gaaagcattg cccgccagtc gaacccggaa gatccagagt cggtgctgac tgaaatggcg
    63781 aaagcaatcc cgctgcgtcg cctcgccgat ccgctggaag tcggcgaact ggcggccttc
    63841 ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga tggcggcagc
    63901 acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc cctgcatttg
    63961 tggggaggat ttcgttttaa acccggttct tattcccttt tcaacttcca aatcaccaaa
    64021 cggtatataa aaccgttact cctttcacgt ccgttataaa tatgatggct attagaaagt
    64081 cattaaattt ataagggtgc gcaatggccg ttaacttact gaaaaagaac tcactcgcgc
    64141 tggtcgcttc tctgctgctg gcgggccatg tacaggcaac ggaactgctg aacagttctt
    64201 atgacgtctc ccgcgagctg tttgccgccc tgaacccgcc gtttgagcaa caatggacca
    64261 aagaaaacgg cggcgacaaa ctgacaataa aacaatctca tgccgggtca tcaaaacagg
    64321 cgctggcaat tttgcagggc ttaaaagcag acgttgtcac ttataaccag gtgactgacg
    64381 tacaaatcct gcatgacaaa ggcaagctga tcccggcgga ctggcaaacg cgcctgccaa
    64441 ataatagctc gccattctac tccaccatgg gcttcctggt gcgtaagggc aacccgaaga
    64501 atatccacga ctggaacgac ctggtgcgct ccgatgtgaa gctgattttc ccgaacccga
    64561 aaacgtcggg taacgcgcgt tacacctatc tggcggcatg gggcgcagcg gataaagctg
    64621 acggtggcga taaagccaaa accgaacagt ttatgaccca gttcctgaaa aacgttgaag
    64681 tgttcgatac tggcggtcgt ggcgcgacca ccacttttgc cgagcgcggc ctgggcgatg
    64741 tgctgatcag cttcgagtcg gaagtgaaca acatccgtaa acagtatgaa gcgcagggtt
    64801 ttgaagtggt gattccgaaa accaacattc tggcggaatt cccggtggcg tgggttgata
    64861 aaaacgtgca ggccaacggt acggaaaaag ccgccaaagc ctaccttaac tggctctaca
    64921 gcccgcaggc gcaaaccatc atcaccgact attactaccg cgtgaataac ccggaagtca
    64981 tggataaact gaaagacaaa ttcccgcaga ccgagctgtt ccgcgtggaa gacaaatttg
    65041 gctcctggcc ggaagtgatg aaaacccact tcaccagcgg cggcgagtta gacaagctgt
    65101 tagcggcggg gcgtaattaa tgtttgcagt ttcatcaaga cgcgtgctgc cgggctttac
    65161 cttaagcctc ggcaccagtc tgctgtttgt gtgcctgatt ttgctgctgc cgctctccgc
    65221 gctggtgatg cagctggctc agatgagctg ggcgcagtac tgggaggtga tcaccaaccc
    65281 gcaggtagta gcggcctaca aagtaacgct gctgtcagcg tttgtggcat cgatttttaa
    65341 cggcgttttt ggcctgctga tggcgtggat cttaacccgc tatcgctttc cgggccgcac
    65401 gctgcttgat gcgctgatgg atttaccctt tgcgctgcca acggcagtcg ccggtttaac
    65461 gctagcctcg ctcttttctg taaacggttt ttacggtgaa tggctggcga agtttgatat
    65521 caaagtcacc tatacctggc tggggattgc ggtggcgatg gcctttacca gcattccgtt
    65581 tgtggtacgt accgtgcagc cggtgctgga agagttaggc ccggaatatg aagaagcggc
    65641 agaaacgctt ggcgcaacgc gctggcagag tttctgcaaa gtggtgctgc cggaactttc
    65701 tccggcgctg gtggcgggcg tggcgctgtc gtttacccgt agtcttggtg aatttggcgc
    65761 ggtgattttt atcgccggaa atatcgcgtg gaagacggaa gtgacgtcgc tgatgatttt
    65821 tgtgcgctta caggagtttg attacccggc agcgagcgcg attgcttcgg tgatcctcgc
    65881 ggcatctctg ctgctgctgt tctcaattaa cactctgcaa agtcgctttg gtcggcgtgt
    65941 ggtaggtcat taatggcgga agttacccaa ttgaagcgtt atgacgcgcg cccgattaac
    66001 tggggcaaat ggtttctgat tggcatcggg atgctggtat cggcgttcat cctgctggtg
    66061 ccgatgattt atatcttcgt acaggcgttc agcaaagggc tgatgccggt tttacagaat
    66121 ctggccgatc cggacatgct gcacgccatc tggctgacgg tgatgatcgc gctgattgcc
    66181 gtaccggtaa acctggtgtt cggcattctg ctggcctggc tggtgacgcg ctttaacttc
    66241 cctggacgcc agttactgct gacgctactg gacattccgt ttgccgtatc gccggtggtt
    66301 gccggtctgg tgtatctgct gttctacggc tctaacggcc cgctgggcgg ctggctggat
    66361 gaacataatc tgcaaattat gttctcctgg cccggaatgg tgctggtcac catctttgtg
    66421 acgtgtccgt ttgtggtgcg cgagctggtg ccggtgatgt taagccaggg cagccaggaa
    66481 gacgaagcgg cgattttgct tggcgcgtct ggctggcaga tgtttcgccg cgtcacattg
    66541 ccgaacattc gctgggcgct gctttatggc gtggtgttga ccaacgcccg cgcaattggc
    66601 gagtttggcg cggtgtcggt ggtttccggc tcgattcgcg gcgaaaccct gtcgctgccg
    66661 ttacagattg aattgctgga gcaggactac aacaccgtcg gctcctttac cgctgcggca
    66721 ctgttaacgc tgatggcgat tatcaccctg tttttaaaaa gtatgttgca gtggcgcctg
    66781 gagaatcagg aaaaacgcgc acagcaggag gaacatcatg agcattgaga ttgccaatat
    66841 taagaagtcg tttggtcgca cccaggtgct gaacgatatc tcactggata ttccttcagg
    66901 tcagatggtc gcgttgctgg ggccgtccgg ttccgggaaa accacgctgc tgcgcattat
    66961 cgccgggctg gagcatcaaa ccagcgggca tattcgcttc cacggcaccg acgtcagccg
    67021 cctgcacgcg cgcgatcgca aggtcggttt cgtgttccag cattacgcgc tgttccgcca
    67081 tatgacggta ttcgacaata tcgcttttgg cctgacggtg ctgccgcgtc gcgatcgccc
    67141 gaatgccgca gccatcaaag cgaaagtgac aaaattgctg gagatggtgc agcttgctca
    67201 tctggcggat cgttatccgg cacagctttc cggcggccag aaacagcgcg tggcgctggc
    67261 gcgcgcgctg gctgtagaac cgcaaattct gctgcttgat gaaccgtttg gcgcgctgga
    67321 tgcgcaggtg cgtaaagagc tgcgtcgctg gctgcgtcaa ctgcatgaag agctgaaatt
    67381 caccagcgtg ttcgtgaccc acgaccagga agaagcgacg gaagtagctg atcgtgtagt
    67441 ggtgatgagc cagggcaata ttgaacaggc tgatgcgccg gagcaggtat ggcgcgaacc
    67501 agcgacccgt tttgtgctcg aatttatggg cgaagtgaac cgcctgcagg gaaccattcg
    67561 cggcgggcag ttccatgttg gcgcacatcg ctggccgctg ggctacacac ctgcttatca
    67621 ggggccggtg gatctcttcc tgcgcccgtg ggaagtggat atcagccgcc gtaccagtct
    67681 cgattcgccg ctgccggtac aggtactgga agccagcccg aaaggtcact acacccaatt
    67741 agtggtgcag ccgctggggt ggtacaacga accgctgacg gtcgtgatgc atggcgacga
    67801 tgccccgcag cgtggcgagc gtttattcgt tggtctgcaa catgcgcggc tgtataacgg
    67861 cgacgagcgt atcgaaaccc gcgatgagga acttgctctc gcacaaagcg cctgataggt
    67921 tgagtgaatg ttaaacgccc ggaggcgctt cccgcgatcc gggcttttta atggcaaggt
    67981 ttgtaacctg tagacctgat aagacgcgca agcgtcgcat caggcaacac cacgtatgga
    68041 tagagatcgt gagtacatta gaacaaacaa taggcaatac gcctctggtg aagttgcagc
    68101 gaatggggcc ggataacggc agtgaagtgt ggttaaaact ggaaggcaat aacccggcag
    68161 gttcggtgaa agatcgtgcg gcactttcga tgatcgtcga ggcggaaaag cgcggggaaa
    68221 ttaaaccggg tgatgtctta atcgaagcca ccagtggtaa caccggcatt gcgctggcaa
    68281 tgattgccgc gctgaaaggc tatcgcatga aattgctgat gcccgacaac atgagccagg
    68341 aacgccgtgc ggcgatgcgt gcttatggtg cggaactgat tcttgtcacc aaagagcagg
    68401 gcatggaagg tgcgcgcgat ctggcgctgg agatggcgaa tcgtggcgaa ggaaagctgc
    68461 tcgatcagtt caataatccc gataaccctt acgcccatta caccactacc gggccggaaa
    68521 tctggcagca aaccggcggg cgcattaccc attttgtctc cagcatggga acaaccggca
    68581 ctatcaccgg cgtttcacgc tttatgcgcg aacaatccaa accagtaacg attgttggcc
    68641 tgcagccgga agaaggcagc agtattcccg gcattcgccg ctggcctgcg gaatatctgc
    68701 cggggatttt caacgcttct ctggtggatg aggtgctgga tattcatcag cgcgatgcag
    68761 aaaacaccat gcgcgaactg gcagtgcggg aaggaatatt ctgtggcgtc agctccggcg
    68821 gcgcggttgc cggagcactg cgggtggcaa aagctaaccc tggtgcggtg gtggtagcga
    68881 tcatctgcga tcgtggcgat cgctaccttt ccaccggggt gtttggggaa gagcatttta
    68941 gtcagggggc ggggatttaa gattattgcg caggcaccgt tttatccgtc tgcgcaatta
    69001 aggcatgtaa aaaaggctgt gcgttctcat cgctcttacc ggggatcttc acaatatacg
    69061 gcttcccggt aatcgatgac gacgaagcaa ccttatcaat aaactgctcg gcagtgtcaa
    69121 tgcggttacg ggtattgccg agcttcagac gcagatgaga aaccgcttca tcgcaggtat
    69181 gttcatcacc gttgcgcaca aatatcaaat cctttttctg tgctaatccc tccagcatgg
    69241 cgttgatgcg ggcttcttca tgaacagtta acttcgcgta agcggggagt gtcattagta
    69301 gtgtaatgac cagacaaatg attttcttca ttgaattacc ttatccttgt tttgtgatag
    69361 ccgatttgac tgccagaatc aggtaaagtt tcggttggaa tacgatgagg atcatcggca
    69421 aggagaaaaa attaaatgag tagtttgttg ttgtttaacg ataagagtag ggcgctgcag
    69481 gcggatatcg tcgccgtgca gtcgcaggta gtgtacggca gcgtaggcaa cagcattgcg
    69541 gtgcctgcta taaaacagaa cggcttgaat gtctttgccg tgccgacggt attgctgagc
    69601 aatacgccgc attatgacac tttctacggt ggtgcgattc cggacgaatg gtttagcggc
    69661 tatttgcgtg cgcttcagga gcgtgatgcg ctgcgccaac ttcgtgctgt aaccacgggc
    69721 tatatgggaa cggcatcgca aatcaaaatc cttgccgagt ggctgactgc gctacgcaaa
    69781 gaccatcctg acctattgat catggtcgat ccggtgattg gcgatattga tagcggaatt
    69841 tatgtcaaac ctgaccttcc cgaggcgtat cgacaatatt tactgccgct ggcgcaagga
    69901 attaccccca atatctttga gctggaaatc ctgaccggta aaaattgccg cgatctcgac
    69961 agtgccattg ctgccgcaaa aagtctgctt tcagacacat taaaatgggg ggtgattacc
    70021 agcgcctccg gtaatgaaga aaatcaggag atgcaggtgg tggtggtcag tgccgacagc
    70081 gtgaatgtca tttcccattc acgggtaaaa accgacctga aagggactgg cgacctgttt
    70141 tgtgctcagc ttatcagtgg tttgctgaaa gggaaggcgt taaacgatgc ggtgcaccgt
    70201 gcggggttgc gcgtactgga agtgatgcgc tatacccagc agcatgagag cgatgaattg
    70261 attttgccgc cgctggcgga agcataaaaa atggcgccga tgg
//
LOCUS       DAJTVJ010000004        64372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000004 DAJTVJ010000000
VERSION     DAJTVJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.004"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="OO448_000239"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="OO448_000239"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6335568.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="OO448_000240"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="OO448_000240"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6335569.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="OO448_000241"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="OO448_000241"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6335570.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="OO448_000242"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="OO448_000242"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR6335571.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="OO448_000243"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="OO448_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR6335572.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /locus_tag="OO448_000244"
     CDS             complement(4810..7044)
                     /locus_tag="OO448_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6335573.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="OO448_000245"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="OO448_000245"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6335574.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="OO448_000246"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="OO448_000246"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6335575.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="OO448_000247"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="OO448_000247"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6335576.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="OO448_000248"
     CDS             complement(12799..14139)
                     /locus_tag="OO448_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6335577.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="OO448_000249"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="OO448_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6335578.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="OO448_000250"
     CDS             complement(15928..16377)
                     /locus_tag="OO448_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6335579.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="OO448_000251"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="OO448_000251"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6335580.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="OO448_000252"
     CDS             complement(17177..17506)
                     /locus_tag="OO448_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6335581.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="OO448_000253"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="OO448_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6335582.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="OO448_000254"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="OO448_000254"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6335583.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="OO448_000255"
                     /gene_synonym="ygdH"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="OO448_000255"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6335584.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21621..22910
                     /gene="sdaC"
                     /locus_tag="OO448_000256"
     CDS             21621..22910
                     /gene="sdaC"
                     /locus_tag="OO448_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6335585.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22968..24335
                     /gene="sdaB"
                     /locus_tag="OO448_000257"
     CDS             22968..24335
                     /gene="sdaB"
                     /locus_tag="OO448_000257"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6335586.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24447..25202
                     /gene="xni"
                     /locus_tag="OO448_000258"
     CDS             24447..25202
                     /gene="xni"
                     /locus_tag="OO448_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6335587.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="OO448_000259"
     CDS             complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="OO448_000259"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6335588.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="OO448_000260"
     CDS             complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="OO448_000260"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6335589.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27677..28993
                     /gene="fucP"
                     /locus_tag="OO448_000261"
     CDS             27677..28993
                     /gene="fucP"
                     /locus_tag="OO448_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6335590.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29026..30801
                     /gene="fucI"
                     /locus_tag="OO448_000262"
                     /pseudo
     CDS             29026..30801
                     /gene="fucI"
                     /locus_tag="OO448_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30910..32328
                     /gene="fucK"
                     /locus_tag="OO448_000263"
     CDS             30910..32328
                     /gene="fucK"
                     /locus_tag="OO448_000263"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR6335591.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32330..32752
                     /gene="fucU"
                     /locus_tag="OO448_000264"
     CDS             32330..32752
                     /gene="fucU"
                     /locus_tag="OO448_000264"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6335592.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32810..33541
                     /gene="fucR"
                     /locus_tag="OO448_000265"
     CDS             32810..33541
                     /gene="fucR"
                     /locus_tag="OO448_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6335593.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="OO448_000266"
     CDS             complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="OO448_000266"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6335594.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34678..35073)
                     /locus_tag="OO448_000267"
     CDS             complement(34678..35073)
                     /locus_tag="OO448_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6335595.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="OO448_000268"
     CDS             complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="OO448_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6335596.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36360..36587)
                     /locus_tag="OO448_000269"
     CDS             complement(36360..36587)
                     /locus_tag="OO448_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6335597.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36779..37984
                     /gene="csdA"
                     /locus_tag="OO448_000270"
     CDS             36779..37984
                     /gene="csdA"
                     /locus_tag="OO448_000270"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6335598.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37984..38427
                     /gene="csdE"
                     /locus_tag="OO448_000271"
     CDS             37984..38427
                     /gene="csdE"
                     /locus_tag="OO448_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6335599.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="OO448_000272"
     CDS             complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="OO448_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6335600.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="OO448_000273"
     CDS             complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="OO448_000273"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6335601.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40667..40743
                     /locus_tag="OO448_000274"
     tRNA            40667..40743
                     /locus_tag="OO448_000274"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40701..40703,aa:Met,seq:cat)
     gene            40777..40853
                     /locus_tag="OO448_000275"
     tRNA            40777..40853
                     /locus_tag="OO448_000275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40811..40813,aa:Met,seq:cat)
     gene            40887..40963
                     /locus_tag="OO448_000276"
     tRNA            40887..40963
                     /locus_tag="OO448_000276"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40921..40923,aa:Met,seq:cat)
     gene            complement(41038..42291)
                     /gene="amiC"
                     /locus_tag="OO448_000277"
     CDS             complement(41038..42291)
                     /gene="amiC"
                     /locus_tag="OO448_000277"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6335602.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42523..43854
                     /gene="argA"
                     /locus_tag="OO448_000278"
     CDS             42523..43854
                     /gene="argA"
                     /locus_tag="OO448_000278"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6335603.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43916..45742)
                     /gene="recD"
                     /locus_tag="OO448_000279"
     CDS             complement(43916..45742)
                     /gene="recD"
                     /locus_tag="OO448_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6335604.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45742..49284)
                     /gene="recB"
                     /locus_tag="OO448_000280"
     CDS             complement(45742..49284)
                     /gene="recB"
                     /locus_tag="OO448_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6335605.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49277..52165)
                     /gene="ptrA"
                     /locus_tag="OO448_000281"
     CDS             complement(49277..52165)
                     /gene="ptrA"
                     /locus_tag="OO448_000281"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6335606.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52341..55709)
                     /gene="recC"
                     /locus_tag="OO448_000282"
     CDS             complement(52341..55709)
                     /gene="recC"
                     /locus_tag="OO448_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6335607.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55722..56045)
                     /locus_tag="OO448_000283"
     CDS             complement(55722..56045)
                     /locus_tag="OO448_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6335608.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56030..56437)
                     /locus_tag="OO448_000284"
     CDS             complement(56030..56437)
                     /locus_tag="OO448_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6335609.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56434..56997)
                     /locus_tag="OO448_000285"
     CDS             complement(56434..56997)
                     /locus_tag="OO448_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6335610.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56988..57458)
                     /locus_tag="OO448_000286"
     CDS             complement(56988..57458)
                     /locus_tag="OO448_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6335611.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57643..58437)
                     /gene="thyA"
                     /locus_tag="OO448_000287"
     CDS             complement(57643..58437)
                     /gene="thyA"
                     /locus_tag="OO448_000287"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6335612.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58444..59319)
                     /gene="lgt"
                     /locus_tag="OO448_000288"
     CDS             complement(58444..59319)
                     /gene="lgt"
                     /locus_tag="OO448_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6335613.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59470..61716)
                     /gene="ptsP"
                     /locus_tag="OO448_000289"
     CDS             complement(59470..61716)
                     /gene="ptsP"
                     /locus_tag="OO448_000289"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6335614.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61729..62259)
                     /gene="rppH"
                     /locus_tag="OO448_000290"
     CDS             complement(61729..62259)
                     /gene="rppH"
                     /locus_tag="OO448_000290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6335615.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62944..63633
                     /gene="mutH"
                     /locus_tag="OO448_000291"
     CDS             62944..63633
                     /gene="mutH"
                     /locus_tag="OO448_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6335616.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63702..>64372
                     /locus_tag="OO448_000292"
     CDS             63702..>64372
                     /locus_tag="OO448_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6335617.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAV"
BASE COUNT        15581 a        17332 c        15787 g        15672 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cttggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 tcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc gcaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaagcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgtgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgcagttaa tatgtcatac aatttatgtt gtaacgcaaa cgtttcccta ttttcataaa
    21241 ccacatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    21301 ttcagttcaa cctacatata ttgcgcgccc cggaagaagt cagatgtcgt ttaatgggca
    21361 aatattgccc ttaaattctc ttttactttt gatttacaga gtaaagcgtt gggataatct
    21421 atcttccaag tagattattg tatttgggat caagatcact gatagataca taacttgtgt
    21481 gtatctttcc gccctcaaat tattacggcg gtaaatgatt aagccatcgc cgatagacag
    21541 atttcatttt tacggtcagg caccttcccg agctgaactg gctaaaagct gaattatttg
    21601 cattcctcca ggagaaatag atggaaacga ctcaaaccag cacgattgcg tcgaaagact
    21661 ctcgtagtgc ctggcgcaag acagacacca tgtggatgct gggcctttac ggcacggcaa
    21721 tcggcgcggg cgtgctgttc ctgccaatca acgccggtgt tggcggtatg atcccgctga
    21781 tcatcatggc tatccttgcg ttcccgatga cattttttgc tcaccgcggc ctgactcgct
    21841 tcgtactgtc tggtaaaaac ccaggcgaag acatcaccga ggttgtagaa gaacactttg
    21901 gtattggcgc aggtaaactg attaccctgc tctacttctt cgctatctac ccgatcctgc
    21961 tggtttatag cgtggcaatc accaatacag ttgaaagctt catgtctcac cagctgggta
    22021 tgacaccacc gccgcgtgcg attctgtcgc tgatcctgat cgtgggtatg atgaccatcg
    22081 ttcgcttcgg tgagcagatg atcgttaaag caatgagtat tctggtattc ccgtttgttg
    22141 gcgtactgat gctgctggct ctgtacctga tcccgcagtg gaacggcgca gcactggaaa
    22201 cgctgtctct ggacactgca tctgcaaccg gaaacggtct gtggatgacc ctgtggctgg
    22261 caattccggt tatggtgttc tctttcaacc actctccgat catctcttct ttcgccgttg
    22321 cgaagcgtga agagtacggc gatatggcag aacagaaatg ctcgaagatc ctggcattcg
    22381 cacacatcat gatggtgctg accgtaatgt tcttcgtctt cagctgtgta ctgagcctga
    22441 ctccggcaga cctggctgcg gctaaagagc agaacatctc gattctgtct tacctggcta
    22501 accactttaa cgcaccggtt atcgcgtgga tggctccgat tatcgcgatt atcgctatca
    22561 ccaaatcctt cctcggccac tacctgggcg cacgtgaagg cttcaacggt atggtgatta
    22621 aatctctgcg tggtaaaggt aagtctatcg aaatcaacaa gctgaaccgt atcactgcgc
    22681 tgttcatgct ggtaacgacc tggattgttg ccaccctgaa cccgagcatc ctgggtatga
    22741 ttgaaaccct gggcggccca atcatcgcga tgatcctgtt cctgatgccg atgtacgcaa
    22801 ttcagaaagt accggcaatg cgtaagtaca gcggtcacat cagcaacgta ttcgttgtcg
    22861 tgatgggtct gattgcaatc tccgcaatct tctactctct gttcagctaa gtcctttcgc
    22921 gccgctttcg ggcggcgctt cctccgtttt aacacgatgt atttcctatg attagcgtat
    22981 tcgatatttt caaaatcggc attggccctt ccagttctca taccgttgga ccaatgaaag
    23041 cgggtaaaca atttaccgac gatctgattg cccgtaatct gcttaaagac gtgacccgcg
    23101 tggtggttga cgtgtacggc tcgctctctc tgaccggtaa aggccaccac actgatatcg
    23161 ccattattat gggcctggcg ggtaacctgc cggataccgt ggatatcgat tccatccctg
    23221 gttttattca ggatgtgaat actcatggtc gcctgatgct ggcaaacggt cagcatgaag
    23281 tggagttccc ggttgatcag tgcatgaact tccatgcaga caacctttct ctgcatgaaa
    23341 acggtatgcg cattaccgcg ctggcgggcg ataaagtcgt ttacagccag acttactact
    23401 ctattggcgg tggctttatc gttgatgaag agcattttgg ccagcagaat agcgcaccgg
    23461 ttgaagttcc ttatccgtac agttcagcag ccgatctgca aaaacattgt caggaaaccg
    23521 ggctgtcact ctctggcctg atgatgaaaa atgagctggc gctgcacagc aaagaagagc
    23581 tggaacagca cctggcgaac gtatgggaag tcatgcgtgg cggtattgag cgcggtattt
    23641 ccaccgaagg cgtgttgcct ggcaaactgc gcgttccacg ccgtgctgcg gcactacgcc
    23701 ggatgctggt cagccaggat aaaaccacca ctgacccgat ggcggttgtt gactggatca
    23761 acatgtttgc actggcagtg aacgaagaga acgctgctgg cggacgcgtg gtgactgcgc
    23821 cgactaacgg tgcgtgcggg attatcccgg cagtgctggc gtactacgac aagttcatcc
    23881 gcgaagtgaa cgctaactca ctggctcgtt acctgctggt agccagtgcc attggttctc
    23941 tttataagat gaacgcgtca atttctggtg ctgaagtcgg ctgccagggt gaagttggtg
    24001 tggcgtgctc aatggcggcg gctggtctgg cagaactgtt aggtgcaagc ccggcgcagg
    24061 tgtgcatcgc ggcggaaatc gccatggagc acaacctcgg tctgacgtgt gacccggtcg
    24121 ccggacaggt ccaggtgcca tgcatcgagc gtaacgccat tgcggcagta aaagcggtga
    24181 acgccgcacg tatggcgcta cgccgtacca gcgagccgcg cgtctgcctc gataaagtta
    24241 tcgaaaccat gtacgaaaca ggtaaagata tgaacgccaa gtaccgcgaa acctctcgcg
    24301 gcggcctggc aatgaagatc gttgcctgcg attaatcgct ctccagaggc ctcgttttgc
    24361 gaggcctctt cccgatttct catccagccg tagcctgttc cggcatcgaa tgttaccctt
    24421 atcgcctgat ctttaagggg gttatcgtgg ctgttcattt gcttattgtc gatgcactga
    24481 atcttattcg tcgcattcat gccgttcagg gatcgccctg tgtcgaaacc tgccagcatg
    24541 cgctcgatca gctcattatg cacagccagc caacccacgc ggtcgccgtt tttgatgatg
    24601 aaaaccgcag tagcggctgg cgtcatcagc gtttaccaga ttacaaagcg ggtcgaccgc
    24661 caatgccgga agagttgcac gacgagatgc ctgcattacg cgccgccttt gagcaacgcg
    24721 gcgtcccgtg ctggtcaacc agcggcaacg aagccgatga cttagccgcc acgctggcgg
    24781 tcaaagtgac acaggccggg catcaggcaa cgattgtttc gacagataaa ggctactgtc
    24841 agttactttc accgacatta cgtattcgtg attacttcca gaaacgttgg ctggatgcgc
    24901 catttatcga taaagaattt ggcgttcaac cgcagcagtt gcccgattac tggggacttg
    24961 cggggatcag cagttcaaag gtaccgggtg ttgcgggaat cggaccaaaa agcgccacgc
    25021 agctgctggt cgagtttcag agtctggaag ggatatatga gaatctggat gcggttgccg
    25081 aaaagtggcg caaaaaatta gaaacccata aagagatggc gtttctgtgc cgcgatattg
    25141 cccgcttaca aaccgatttg catatcgacg gcaatttaca gcaattgcgg ttggtacggt
    25201 aacggcgagc cggatgcaaa cgattatcaa cacattgaat ttaatgcact tatataccgg
    25261 ataagacgcg gctagcgtcg tatccggcat tatcacatca gcgcatttac caggcggtat
    25321 ggtaaagctc tacaatatcc tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat
    25381 catccagtgc cgcctgcgcc agtgccggaa tgtcttcctt gcgtacacca acatcacgca
    25441 aatgtggcgg aataccgaca tcacggttga gagcaaacac cgcttcaaca gcggcattac
    25501 gcgcctcttc caggctcata ccttccactt tcacgcccat aacgcgcgcg atatcgcggt
    25561 acttctcacc ggtaaagtca gcgttatagt gcatgacatg cggtaacagg atggcgttcg
    25621 caacaccgtg tggagtgttg taaaacgcgc ccagtggatg cgccatacca tgcaccaacc
    25681 ctaacccaac attcgagaag cccatacccg caacatactg cccgagcgcc atttcttctc
    25741 cggcatcctt atcaccagca accgatcctc gcagcgcccc agcaatgatt tcaatcgctt
    25801 taatgtgcag tgcatcggtt agcgcccacg cgccacgggt aatatacccc tcaatagcat
    25861 gagtgagcgc atcgacaccc gtcgcagctt tcagcgctgg aggcatacca tccatcatgt
    25921 cagcgtcaat aaacgccacc tgcgggatat catgcggatc aacgcaaaca aacttgcgcc
    25981 gtttctcttc gtcagtgatc acgtagttaa tggtcacttc tgccgcagta cctgctgtgg
    26041 taggaattgc cagaatcggt acactgggtt tattggtcgg ggaaagccct tccaggctac
    26101 gcacatcggc aaactccgga ttgttgctga taatgccaat cgctttacaa gtatcctgtg
    26161 gagaaccacc accaatagcg atcaggtaat ccgcgccgct attctggaat acaccgagcc
    26221 cttctttgac gacagtaatt gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca
    26281 gccctgcagc atccatctta tcggtcactt tcgccaccac gccgcattgc accagcgttt
    26341 tatcggtgac gatcagcgcc ttctgataac cacggcgttt cacctcatcg gttaaagccc
    26401 caacagcacc ccgaccaaac catgccgttt cgttcagaat cattctgtta gccatcatcc
    26461 ttctccttgt tgctttacga aattactctt caatccgtaa cccataggtt ttgaatttct
    26521 ccagcactac ggcaatctct tcatcgctca gcactggcac cgggtccgta atcgccaggg
    26581 tcgccacgta aagttgcgcc agcacttcaa cttcatgcgc cagccataac gctttttcca
    26641 gattcacctc acaagcgata agcccatgat gttgtaacaa agttgcctta cgatttttga
    26701 gagccagcgc aacatgttca gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag
    26761 gaatagaatt accacctgcc gccgcaatca tgtagtgaat agcggggatc ggtcggttaa
    26821 gaatggaaac tgctgtgcaa tgaacggcat gattgtgaac aaccgcgttg gcatccggtc
    26881 tgctttgata ggccgccata tggaaacgcc attcgcttga ggggagcttt ccttcctcat
    26941 gtttaccgtt gccatcaata aagacaatat gcgactccgt cagtttttca tatggaatgc
    27001 ctgtaggcgt aatcagcatc ccatcctgat aacgtacact gacgttcccc gctgtcccct
    27061 ggttcagtcc caggcgggtc atttccaggc aagtgtcaat aatctgacga gcaagtttat
    27121 ttcgttccat tagctacctc tctctgattc aaaacagggc aataatgttg ttcctttcac
    27181 actattgaat tagccgttta attacccacc atcttcttcc tgattaacaa gaaagaaatt
    27241 cacaagctta tattttgtga cctagctcaa ctaatcacag taaataactg caagttctct
    27301 ttttataacc ccattaaaaa tgaccgctct taaaaatatt tatcaaaacg gtcatttttc
    27361 tattcaccca agccaggaat gcccgttttc ggcataaaca attaatacag tcatctggtt
    27421 tgtgtttttt atgatttatt ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg
    27481 tagtcacata aagtcacctt ccagctaata agtgtgaccg ccgtcatatt acagagcgtt
    27541 ttttatttga aaatgaatcc atgagttcat ttcagacagg caaatattca ctgatatgaa
    27601 gcccgaactc gctggttttg cacttttgaa aacataaccg attacgtgct taagcttctg
    27661 aacctaagag gatgctatgg gaaacacatc aatacaaacg cagagttacc gtgcggtaga
    27721 taaagatgca gggcaaagca gaagttacat tattccattc gcgctgctgt gctcactgtt
    27781 ttttctttgg gcggtagcca ataaccttaa cgacatttta ttacctcaat tccagcaggc
    27841 ttttacgctg acaaatttcc aggctggcct gatccaatcg gccttttact ttggttattt
    27901 cattatccca attcctgctg ggatattgat gaaaaaactc agttataaag caggaattat
    27961 tactgggtta tttttatatg ccctgggcgc tgcattattc tggcccgctg cagaaataat
    28021 gaactacacc ttatttttag ttggcctatt tattattgca gccggattag gttgtctgga
    28081 aactgccgca aacccttttg ttacggtatt agggccggaa agcagtggtc acttccgctt
    28141 aaatcttgcg caaacattta actcgtttgg cgcaattatc gcggttgtct ttgggcaaag
    28201 acttattttg tctaacgtgc cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga
    28261 gcaattgagt gcgtataaac acagcctggt attatcggta cagacacctt atatgatcat
    28321 cgtggctatc gtgttactgg tcgccctgct gatcatgctg acgaaattcc cggcattgca
    28381 gagtgataat cacagtgacg ccaaacaagg atcgttctcc gcatcgcttt ctcgcctagc
    28441 gcgtattcgc cactggcgct gggcagtatt agcgcaattc tgctacgttg gcgcacaaac
    28501 ggcctgctgg agctatttga ttcgctacgc tgtagaagaa attccaggta tgactgcagg
    28561 ctttgccgct aactatttaa ccggaaccat ggtgtgcttc tttattggtc gtttcaccgg
    28621 tacctggctc atcagtcgct tcgcaccaca caaagtcctg gccgcctacg cattaatcgc
    28681 tatggcactg tgcctgatct cagccttcgc tggcggtcat gtgggcttaa tagccctgac
    28741 tttatgcagc gcctttatgt cgattcagta cccaacaatc ttctcgctgg gcattaagaa
    28801 tctcggccag gacaccaaat acggttcgtc cttcatcgtt atgaccatta ttggcggcgg
    28861 tattgtcact ccggtcatgg gttttgtcag tgacgcggcg ggcaacatcc ccactgctga
    28921 actgatcccc acactctgct tcgcggtcat ctttatcttt gcccgtttcc gctctcaaac
    28981 ggcaactaac tgaacatatt ttccgaataa agtgaggaat ctgtaatgaa aaaaatcagc
    29041 ttaccgaaaa ttggtatccg cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg
    29101 cttgaagaac aaacaatgaa tatggcgaaa gctacggccg cactgctgac cgagaaactg
    29161 cgccatgcct gcggagctgc cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg
    29221 gctgaagccg ctgcttgcga agaaaaattc agcagctaga atgtaggcct caccattacg
    29281 gtaacgcctt gctggtgcta tggcagtgaa accatcgaca tggatccaac ccgcccgaaa
    29341 gccatttggg gctttaacgg cactgaacgt cccggcgctg tttacctggc agcggctctg
    29401 gcagctcaca gccagaaagg catcccggca ttctccattt acggtcatga cgttcaggat
    29461 gccgatgaca catcgattcc tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc
    29521 ggtttggccg tcgccagcat gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg
    29581 ggtatcgccg gttctattgt tgatcacaac ttctttgaat cctggctggg aatgaaagtc
    29641 caggcggtgg atatgaccga actgcgtcgc cgtatcgatc agaagattta cgacgaagcc
    29701 gaactggaaa tggcactgac ctgggctgat aaaaacttcc gctatggcga agatgaaaat
    29761 aacaaacagt atcaacgcaa tgccgagcaa agccgcgcag ttctgcgcga aagtttactg
    29821 atggcgatgt gtatccgcga catgatgcaa ggcaacagca aactggccga tattggtcgc
    29881 gtggaagaat cacttggcta caacgccatc gctgcgggct tccaggggca acgtcactgg
    29941 accgatcaat atcccaatgg tgacaccgcc gaagcgatcc tcaacagttc atttgactgg
    30001 aatggcgtgc gcgaaccctt tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca
    30061 atgctaatgg gtcaccagct caccggcacc gctcaggtat ttgccgatgt gcgtacctac
    30121 tggtcaccag aagcaattga gcgtgtaacg gggcataaac tcgatggact ggcagaacac
    30181 ggcatcatcc atttgatcaa ctcaggttct gctgcgctgg acggttcctg taaacaacgc
    30241 gacagcgaag gtaacccgac gatgaagcca cactgtgaaa tctctcagca agaggctgac
    30301 gcttgcctcg ccgctaccga atggtgcccg gcgatccacg aatacttccg tggcggcggt
    30361 tactcttccc gcttccttac cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc
    30421 atcaaaggcc tgggatcggt actgcaaatc gcggaaggct ggagcgtgga attgccgaag
    30481 gatgtgcatg acatcctcaa caaacgcacc aactcaacct ggccaaccac ctggtttgca
    30541 ccgcgcctca ccggtaaagg gccgtttacg gatgtgtact cggtaatggc gaactggggc
    30601 gctaaccatg gggttctgac catcggccac gttggcgcag actttatcac tctcgcctcc
    30661 atgctgcgta tcccggtatg tatgcacaac gttgaagaga ccaaagtgta tcgtccttct
    30721 gcctgggctg cgcacggcat ggatattgaa ggccaggatt accgcgcttg ccagaactac
    30781 ggtccgttgt acaagcgtta atacattact ccctgatgtg atgcccggtc gctccggcta
    30841 ccgggcctga acaagcaaga gtggttagcc ggataagcaa tgttatccgg ctatattgca
    30901 ggagcgatta tgaaacaaga agttatcctg gtactcgact gtggcgcgac caatgtcagg
    30961 gccatcgcgg ttaatcggca gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc
    31021 gatatcgcga tggaaaacaa cacctggcac cagtggtctt tagacgccat tttgcaacgc
    31081 tttgctgatt gctgtcggca aatcaatagt gaactgactg aatgccacat ccgcggtatc
    31141 gccgtcacca cctttggtgt ggatggcgct ctggtagata agcaaggcaa tctgctctat
    31201 ccgatcatta gctggaaatg tccgcgaaca gcagcggtta tggacaatat tgaacagttg
    31261 atctccgcac agcggttgca ggctatttct ggcgtcggag cctttagttt caatacgtta
    31321 tataagttgg tgtggttgaa agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg
    31381 ctctttattt cgtcgctgat taaccaccgt ttaaccggcg aattcactac tgatatcacg
    31441 atggccggaa ccagccagat gctggatatc cagcaacgcg atttcagtcc gcaaatttta
    31501 caagccaccg gtattccacg ccgactcttc cctcgtctgg tggaagcggg tgaacagatt
    31561 ggtacgctac agaacagcgc cgcagcaatg ctcggcttac ccgttggcat accggtgatt
    31621 tccgcaggtc acgataccca gttcgccctt tttggcgctg gtgccgacca aaatgaaccc
    31681 gtgctctctt ccggtacatg ggaaatttta atggtccgta gcgcccaggt tgatacttcg
    31741 ctgttaagtc agtacgccga ttctacctgc gaactggata gccaggcagg gttgtataac
    31801 ccaggtatgc aatggctggc atccggcgtg ctggaatggg tgagaaaact gttctggacg
    31861 gctgaaacac cctggcaaat gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat
    31921 ggtgtgaaaa tgcagtgtga tttattgtcg tgtcagaacg ctggctggca aggagtgacg
    31981 cttaatacca cgcgggggca tttctatcgc gcggcgctgg aagggttaac tgcgcaatta
    32041 cagcgcaatc tacagatgct ggaaaaaatc gggcacttta aagcctctga attattgtta
    32101 gtcggtggag gaagtcgcaa cacattgtgg aatcagatta aagccaatat gcttgatatt
    32161 ccggtaaaag ttctcgacga tgccgaaacg accgtcgcag gagctgcgct gttcggttgg
    32221 tatggcgtag gggaatttaa cagcccggaa gaagcccgtg cacagattca ttatcagtac
    32281 cgttatttct acccgcaaac tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac
    32341 aatttcgccg ttaatttctc ccgaactatt gaaagtgctg gcagagatgg gacatggaga
    32401 tgaaattatt ttttccgatg ctcactttcc cgcccattcg atgggaccgc aggtgatccg
    32461 cgctgatggc ctgttggtga gcgacttgct ccaagcgatt atcccattat ttgagctgga
    32521 cagttatgcc ccgccgctgg tgatgatggc agcggtagaa ggtgacactc tcgatcctga
    32581 agtagaacga cgttaccgta atgcgctttc actacaagcc ccgtgtcctg acatcatccg
    32641 catcaatcgt tttgcgtttt atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg
    32701 cgaacgagcg aagtacggga atattctttt aaaaaaaggg gtaacaccgt aatctcatat
    32761 cggtacgccc gcatgacgcg ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc
    32821 acgccagcaa gcgatagtcg acctgctgct gaaccatacc agcctgacca cggaagctct
    32881 ctctgaacag ctaaaggtca gtaaagaaac cattcgtcgc gatctcaatg aattacagac
    32941 gcagggtaaa attctgcgca atcatggacg cgctaaatat atccaccgtc aaaatcaaga
    33001 cagtggcgat ccctttcaca tcaggctgaa aagccattat gcgcataaag cagatatcgc
    33061 gcgcgaggcg ctcgcgtgga ttgaagaagg gatggtgata gccttagacg ccagttcaac
    33121 ttgctggtat ctggcacgcc agttgcctga catcaacatt caggtcttca ccaatagcca
    33181 tccgatttgc catgaactcg gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac
    33241 acttgagcgc aaatatggct gttacgtcaa tccctcgctg atttcccaac ttaaatcgct
    33301 ggaaatcgat ctgtttattt tttcttgtga agggatcgat agcagcggcg cactgtggga
    33361 ctccaatgcg atcaacgctg attacaaatc gatgctatta aaacgtgccg cgcaatcgtt
    33421 gttattgatt gataaaagta aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga
    33481 tgaggtaacg cacattattt ctgatgagcg ccaggttgca acttctttgg taacagcctg
    33541 acggaaaggg tagcaggccg aagacgacgc cccggccttg cctgttatcg ctcgtcgcga
    33601 cgaccaccca ccgcagccca gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga
    33661 tacaactgcc gcgcctgaat ctgagcattt atgccatgtt catcaagctg tgcctgaata
    33721 tacgccagat tgtgtgatac ttcttcgtag cgttttttca tcggcagttt gagattgaaa
    33781 atggtttcac ggcaccagcc attaaccagc cactgcgcca tcaatgccgc aactttagcc
    33841 ggtttttcaa ccatatcgca taccatccag gagatattgc tgcgtgtcgg acggaatttg
    33901 aaaccgtctt cccgcagcca cgtcacctgc ccggtatcca tcagactttg cgccatcggg
    33961 ccgttgtcga cggaataaac ccacatgttg cgcttcacca gttggtaggt ccagccgcca
    34021 gggcaagcgc ctaaatccac cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc
    34081 gcaggaataa acacatgaaa tgcctcttcc agtttgagcg tggaacgact cggcgcctct
    34141 gccggaaatt tcaggcgcgg aatgcccata tagaacggcg aattattgtt gctgtatgag
    34201 taaccggtat agcagcagcc tggtgcaata aagaatacat gcacaaccgg acgcttcggc
    34261 gtttcatagt tcgccagcac cccggcatcg cgcaaggcag cgcgtagcgg cacggtgaat
    34321 ttacggcaga atttcagcaa ctctttgctt tcgttggtat cggcaacttc aacacgcagt
    34381 tcaccgccct tctctactac gccctgcaac atgtcgacaa tgggggtaat acgatcttct
    34441 ggcggcaaat gctgcaagag ttcccccacc acaaaccact ggcgggcaaa aattaatgaa
    34501 ctgaacggca gctcacggat taacttatcg ccatcatcag gttgataaca ttcataaatg
    34561 acataacccg cattctcttt cacgcgggca aaaccgaaaa tttcccgctg gccggcttta
    34621 tcggtaattt ctgcggcgca ctctttttca aagcccggac ggcacagcaa tacaacctta
    34681 ttcatgactt acgcccttgc gctttaaacg gatagcacca actaacatta acgcccagcc
    34741 cgcgaggaag ctcacgccgc caaccggtgt gacaaatgcc cacaaacgca gatgggacag
    34801 cgccaggcaa taaaggctgc cgctgaacaa caccgtgcct aacgcgagga aaacgctact
    34861 ccagtaaaac cagatactga tgcgacgctg cattgccacc gccagaccta agatcgccag
    34921 cgtatgaaac gcctggtatt cgaggccggt ctggatccag cccatctcaa cggcccccat
    34981 ggttttactt aacacatgcg cgccaaaagc gcccagagcc acaaaaatga agccgctaat
    35041 ggcggcgaaa atcagcataa aacggctggt catggtcgta ccctacgtaa attattgttc
    35101 ataacgaaag cggaattttt cttgttcagc agcggctttc gccaggatcc attggcgaaa
    35161 ggcggctatt ttacccagtt ctgcctggct gtcatgacaa accagataaa aagcgttttt
    35221 actgaccaga acatcattaa acgggcaaac aagacgtccg gcctcgattt cagattgcgc
    35281 catcacgtta tttgccagcg ccactccctg cccgtgaata gccgcttgca gcaccatggc
    35341 gctatgacta aaaattggcc cttgctgaac gttgatatga tttaacccca actgtcgggt
    35401 atatgtctgc cagtcacggc gtgacgcatc atgtaataac gtatgtttag ccagatcttc
    35461 cggggtcttc aagggttttt cgcctgtcag cagtagcggc gaacacaccg gcaataaata
    35521 ttcggcgtac agtttttcca cccgtagccc cggccagttg ccccgaccat aaaatatcgc
    35581 cacatcaaca tcatccgcca gcttatcttc ctgacgatca accgcctgga ttcgaacgtc
    35641 aattcccgga taagctgaat taaagctgga aagtcgcgga accaaccaat gaatggcgaa
    35701 actggggagt aaactgaccg tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt
    35761 cgcttcggtt aattgcgaaa atatctcttt gatatcgagg aaatagcttt gcccttcctc
    35821 ggtcagcagg agtgaacgat tacggcggcg gaacagtttt agccccaaaa aatcctcaag
    35881 agacttgatt tgatgactta ctgcggcctg ggtcacgaaa agctcttctg ctgcgcgagt
    35941 gaaacttaaa tggcgtgctg cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg
    36001 tttagacatg gcttttaaac tttgatgtta aattaattta acaattagat cacactgtgt
    36061 aacctattag tttttttaat ctgagccatt ataaattgtc cgttgagctt ctaccagcaa
    36121 atacctatag tggcggcact tcctgagccg gaacgaaaag ttttatcgga atgcgtgttc
    36181 tggtgaactt ttggcttacg gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga
    36241 ttcagaccat ggtagcaaag ctaccttttt tcacttcctg tacatttacc ctgtctgtcc
    36301 atagtgatta atgtagcacc gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat
    36361 tactgatcca gttcgaccat ctctttcacg tcagtacggt tgatctgctg tttgttgcca
    36421 ttagcgtctt tatacgaaat cataccggta tcgttatcag tctgtggttt gccgtcagag
    36481 acgatggtac gtccgtcatt ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg
    36541 gcaaaagtca gcatacaggc agaaataatt gcggcagtct ttttcatcat cttctcctta
    36601 aagcgattaa taaatcttaa tagtattgat taacaggcta aaattaacgc ctaacactat
    36661 tcagcatatg ttacttacgc ggcttcgcca ggatatccag ataattctga tggttagcac
    36721 tctccttgta tcaaagtgaa ttttgcgtca cgatcggtgc atcaagccga ggagtaccat
    36781 gaacgttttt aatcccgcgc agtttcgcgc ccagtttccc gcactacagg atgcgggcgt
    36841 ctatctcgat agcgccgcga ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca
    36901 gttttacagc ctgagcgccg gaaacgtcca tcgcagccag tttgccgaag cccaacgcct
    36961 gaccgcgcgt tatgaagctg cacgagagaa agtggcgcaa ttactgaatg caccggatga
    37021 taaaactatc gtctggacgc gcggcaccac tgaatccatc aacatggtgg cacaatgcta
    37081 tgcgcgtccg cgtctgcaac cgggcgatga gattattgtc agcgtggcag aacaccacgc
    37141 taacctcgtc ccctggctga tggtcgccca acaaactgga gccaaagtgg tgaaattgcc
    37201 gcttaatgcg cagcgactgc cggatgtcga tttgttgcca gaactgatta ctccccgtag
    37261 tcggattctg gcgttgggtc agatgtcgaa cgttactggc ggttgcccgg atctggcgcg
    37321 agcgattacc tttgctcatt cagccgggat ggtggtgatg gttgatggtg ctcagggggc
    37381 ggtgcatttc cccgcagatg ttcagcaact agatatcgat ttctatgctt tctctggtca
    37441 caaactgtat ggcccgacgg gtatcggcgt gctgtatggc aaatcagaac tgctggaagc
    37501 gatgtcgccc tggctgggtg gcggcaaaat gattcacgaa gtgagttttg acggcttcac
    37561 gactcaatct gcgccgtgga aactggaagc aggaacgcca aatgtggctg gtgtcatagg
    37621 attaagcgcg gcgctggaat ggctggcaga ttacgatatc aaccaggcag aaagttggag
    37681 tcgcagcctg gcgacgctgg cggaagatgc gctggcaaaa cgtcctggct tccgctcatt
    37741 ccgctgccag gattccagcc tgctggcctt tgattttgct ggcgttcatc acagcgatat
    37801 ggtgacgctg ctggcggagt acggtattgc cctgcgggcc gggcaacatt gcgctcagcc
    37861 gctactggca gaattaggcg taaccggcac actgcgcgcc tcttttgcgc catataatac
    37921 aaagagtgat gtggatgcgc tggtgaatgc cgttgaccgc gcgctggaat tattggtgga
    37981 ttaatgacga acccgcaatt cgccggacat ccgttcggca caaccgtaac cgcagaaacg
    38041 ttacgcaata ccttcgcacc gttgtcgcaa tgggaagata aatatcgcca gttgatcatg
    38101 ctggggaaac agcttccggc tttgccagac gagttaaaag cgcaggctaa agagattgcc
    38161 ggatgcgaaa accgcgtctg gctgggatat accgtgactg aaaacggtaa aatgcacttc
    38221 tttggcgata gcgaagggcg cattgtgcgc ggcctgctgg cggtgctgtt gactgccgtt
    38281 gaggggaaaa ccgccgccga attgcaggca cagtcgccgc tggcattgtt tgatgagctg
    38341 ggattgcgtg cgcaacttag cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg
    38401 attatcgctg cgacgaagca ggtttaagca tagaaccggg tcggataaga cgttcgcgtc
    38461 tcatccgacc tgattgttta accctgacgc gccgctttcg ccatcatctt cttcagcgca
    38521 tgagaaaccg caacaaaccc aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat
    38581 gcacaatcca tccgcttcgg cccttctgcc gtggctttca tcgcacacac agtaccgtct
    38641 gactgcgggt acaccagcgc ttcagtagaa aacacgcaat cgacgccgag cttaccttta
    38701 ctgtttttca ctacgccaaa atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga
    38761 tcctgaatcg ttttcgccag atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc
    38821 gcgccaccgg tcgtgaccag cgggatttta ttgcgccgac aataagcaat cagcgccgct
    38881 ttgggccgta cactatcaat ggcatcaatc acgtacgaat aacccacgct catatactgc
    38941 gctacgttat ccggcgtcac gaaatcatcc accaccgtta cacggcactc cgggttaatc
    39001 tggcgaatac gctccgccat gacttccgct tttgccagcc cgacgttatc gcgcagagcg
    39061 tgaatttgcc gattggtatt ggtgacgcac acatcatcca tatcgataag cgtgattgcg
    39121 ccaatccccg tgcgcgccag cgcttccgcc gcccaggaac cgacaccacc gataccaacc
    39181 acacaaatat gcgcgtcagc aaacagttgc aacgcttttt caccatacag acgcgctgtg
    39241 ccaccaaaac gctgacgcca tgcatcacta attaccacag acatagcacc tcagaattaa
    39301 aaagggtgag gttatccctc accctgtcgt atccgtaaaa acggtataca gaataacaca
    39361 tcagccgcta aagacgttac ctgcgcccgg ggcggttttc agcacccaga cacgtccata
    39421 gtggttgtac caacctgcgc ggtgtccggc ttccggcccg atcccttgat agatatcgaa
    39481 gtgttggcct ttgattgcac caccgacatc cagcgccacc atcagacgca gttcgtactg
    39541 accattaaat ttgccgttat tatccagcaa cggcacttct gccagcaagg tagtacctgg
    39601 cggaataatg gaacgatcag aggcaactga cgcgcgacca accagcggca ccgcacttgc
    39661 ccctttcacc ggtgcaaaag attgcggttt aaagaagacg aaagacgggt tctgttccag
    39721 cagctcgcga acctcggctt cactgtgtgt ttcgccccag tgacgaatcg cctgcatcga
    39781 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    39841 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    39901 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    39961 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    40021 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    40081 aattaccggc gtgtaatagc ctgtaaactg cacgttacca tagttgtcgg caccttccat
    40141 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    40201 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    40261 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    40321 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    40381 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    40441 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    40501 acaaaccgcc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    40561 ataggcttta gtgatttgtt tttgttcaaa atcatgccag atccgtgatc ggggtaaaaa
    40621 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggaggcg gggtggagca
    40681 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    40741 ccaattaaaa tttgatgaag taaagcagta cggtgacgcg gggtggagca gcctggtagc
    40801 tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa ccaatcaaat
    40861 ttgatgaagt aaaagcaata cggtgacgcg gggtggagca gcctggtagc tcgtcgggct
    40921 cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa ccaattattg aacaccctaa
    40981 cgggtgtttt tttgtttcta ctctcccata aaaaaagcgc cattcagcgc ctttttatca
    41041 tccccttctc gccagcgttg ccccatcggc aaaatacgct ttaatccccg caagaataga
    41101 ctccgcaact tcctgctgga aagtcgccgt tttcagttta cgctcttcct caacgttact
    41161 gataaacgcc gtttcgacca gaatggaggg aatatctggt gcctttagta cggcaaaccc
    41221 ggcctgttca acttgatttt tatgcagctt gttgatttta ccgagcttat tcagcactgc
    41281 tttaccaaac ttaaggctgt cagcaatggt cagcgactgc accatatcga acatggtgtg
    41341 gtcgacatag cggtcaccgc ttttgctcac gccaccaatc aagtccgagg cgttctgggt
    41401 ttgtgccaga tattttgccg cagtactggt tgcacctttg gttgagagcg caaacaccga
    41461 ggaaccgctc ggctggcgac tggtaaaggc gtcggcatgg atagagacaa acaggtcagc
    41521 acgctgtttc tgggcttttg ctacgcgcac ttgcaacgga atgaagatgt cttcattgcg
    41581 cgtcatgtac accttcatat tgccctcttt ctcgatcaga gagcgcaaac ggcgagctat
    41641 ttgcaatacc acatcttttt cgcgcgtttt gtatttcccc accgcaccgg agtcttcgcc
    41701 accgtggcca gggtcaagca taatgacaat cggacgatcg cgccctgctt tacctggttg
    41761 tggaccactt tgcgctggcg gcacctgctt ttcgaggtcg cctttgttgt aatcctccag
    41821 cagcgccagc agcgggtcct gcatatcctg tgcattggcc ggatagaggt ccatcaccag
    41881 acgctcttta aacccggcga ccggcgcaag ggcaaacagc tgcggtttta cgttttgctt
    41941 taattcaaaa accatccgta cggtttgcgg gtcaaattgc ccgacgcgcg ccgacttgat
    42001 gaacgggtcg tctgcacgaa tttgtgccgc catccccttg agtaccgagt tcaggtttac
    42061 atcttcgata tccaccacca cgcgttcagg gttactcaac gcgaactgct tatatttcag
    42121 ctgacgattt gattctaccg tcacgcgggt gtagctggac gcaggccaga cgcgcaccgc
    42181 cacgacctgg ctaaccgcag ccaggctgac ctgacttacg ctcaatagcc acatagcgcc
    42241 cgcgccttgc agtaaacgac gacggctgat tgcagtgttg gatcctgaca tgcctctccc
    42301 gagcaaaaga aatctaagct gtgtaacaag taaacgacta atttgaccgg aaactttagc
    42361 gaaagacgca taatctgtca tctaataaac ggtaaacatt ctttttatat tcacggcatt
    42421 actgataaaa aagtcgctct cgcataaaat ttacacttgc accctgcgaa aaaacagaat
    42481 aaaaatacaa taatttcgaa taatcatgca aaggggtgtg ccgtggtaaa ggaacgtaaa
    42541 accgagctgg tcgagggatt ccgccattcg gttccctata tcaataccca ccggggaaaa
    42601 acgtttgtca tcatgctcgg cggtgaagcc attgagcatg agaatttctc cagtatcgtt
    42661 aatgatatcg ggttgttgca cagcctcggc atccgtctgg tggtggtcta tggcgcacgt
    42721 ccgcagatcg acgcaaatct ggctgcgcat caccacgaac cgctgtatca caagaatata
    42781 cgtgtgaccg acgccaaaac actggaactg gtgaagcagg ctgcgggaac attgcaactg
    42841 gatattactg ctcgcctgtc gatgagtctc aataacacgc cgctgcaggg cgcgcatatc
    42901 aacgtcgtca gtggcaattt tattattgcc cagccgctgg gcgtcgatga cggcgtggat
    42961 tactgccata gcgggcgtat ccggcggatt gatgaagacg cgatccgtcg tcaactggac
    43021 agcggtgcaa tagtgctaat ggggccggtc gctgtttcag tcactggcga gagctttaat
    43081 ctgacctcgg aagagattgc cactcaactg gccatcaaac tgaaagctga gaagatgatt
    43141 ggtttttgct cttcacaggg cgtcactaat gacgacggtg atattgtctc agaacttttc
    43201 cctaacgaag cgcaagcgcg ggtagaagcc caggaagaga aaggcgatta caactccggt
    43261 acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca gcggcgtgcg tcgctgtcat
    43321 ttaatcagtt atcaggaaga tggcgcgctg ttgcaagagt tgttctcacg cgacggtatc
    43381 ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc gcgcaacaat caacgatatt
    43441 ggcggtattc tggagttgat tcgcccgctg gagcagcaag gtattctggt gcgccgttct
    43501 cgcgagcagc tggagatgga aatcgacaaa ttcaccatta ttcagctcga taacacgact
    43561 attgcctgcg ccgcgctcta tccgttcccg gaagagaaga ttggggaaat ggcctgtgtg
    43621 gcagttcacc cggattaccg cagttcatca cggggcgagg ttctgctgga acgcattgcc
    43681 gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc tgaccacgcg cagtattcac
    43741 tggttccagg aacgtggatt taccccggtg gatattgatt tactgcccga gagcaaaaag
    43801 cagttgtaca actaccagcg taaatccaaa gtgttgatgg cggatttagg gtaagcgatg
    43861 aaaatcgccg gatgcggcag gcgtaacact cgtgcgtcgc atccggcaat tacgtttatc
    43921 cccgcgaact aaacaacgcc gccagaccac tgcgccgctc agtacgagtg gcgattgccg
    43981 cacttaatat acgctcatcg gcatacagcg acagacggcg acgcgcgcgg gtcaccgcgg
    44041 tgtataccag ctctcgcgtt actatcggcg tgcgttggct cggtaaaatc aacgccgcat
    44101 ggtcgaactc cgatccctgc gatttatgta ccgtcatcgc ccacgtagtt tcgtgctctg
    44161 gcaagcgact cggttgcaca gacttaatat tgccgtccgg catcgcaaac cagacgcgcg
    44221 tcccctgccc gcgatccagc gcaataccga tatcgccatt aaacaaccca agcgcgctgt
    44281 cattacgggc aatcatcacc ggtcgacctt cgtaccaacg agagtgcgaa tgacgatgaa
    44341 ttttgcgctt ctgttgcata aactgctcaa ttcgctcatt cagtccagcc acgccaaatg
    44401 gcccttcccg cagggcgcac aaaagttggt actcattgaa cgcctgaatg attaaatccg
    44461 gctcggcacg cgcttgcagc agatccagat aacgtccata acccgcaaga gcttcctcaa
    44521 gcatcgcaat ataatcttcg ccgctctgta aaagccgttt ttcaatatca gtaaaatcct
    44581 gctgaaaaac ggttttcact gccgttttat caccacggtt gatcgccgca gctaactgac
    44641 caatgccaga atcgctgccg aaacgatagc ttttttgcag caggcagaga ctgtcgcgca
    44701 aagatgccgc ttctgtgccg gttcctgccg gaacgtgagt tcccgtcagg cggcttagct
    44761 gcccggcacg ctcggcggta aagccggcat tggcataagc gcagatatcg cccagcacag
    44821 ccccagcctc aaccgaggcc agttgatcac gatcgccgag aaagatcact cgcgcatgat
    44881 cgggcaaggc gtcgatcagt ctcgacatca taggcagatc gatcattgac gcttcatcta
    44941 ccaccagcac atcaagatgc agcgggttac cggcatgatg acgtaaacgc tggctacccg
    45001 gctgcgcgcc cagcaatcgg tgcaaagtgc tggcatcttc cggaatgcgt ttcttttgtt
    45061 catcggtcag cggtaactgt cgcaaagcct tgccgagaga ttcggttaag cgcgcggcag
    45121 ctttacccgt tggtgcagcc agacggatac ggcagcgttc gccgtcggcc atttgaatta
    45181 acgctgccag caacttcgcc acggtggtcg ttttaccggt gcctgggccg ccggaaatca
    45241 ccgagatccg ccgcgtcagc gccactgctg ccgccacttt ttgccagtta atttcatcgc
    45301 ttactggaaa aagtttgtcc agggtttgcg ccagtagagc ttcatcaacc tcaatggcat
    45361 gattcacctc attgaagaag cgcgctaccg tccgctcgtt acaccacatg cgattcaaat
    45421 aaagacgatc gccacagagg atcatcggcg tgggttcatc tcccctgctg accgcttgag
    45481 aagccagcaa ggattcttcc caattttgta gctcaccgat ttcactgaca caggtcgcca
    45541 acagcggatg cgacgcctcg ttattttcca gtcgtgaaag cggcaaacag acgtgtccct
    45601 ctccggcatc atgacttaac agtgccgccg cgagggtgac ggcaggatgt tcatctcccg
    45661 ccacggtcag ggcaaattgc acatccagcg ggcgtagctg tttgtgctcc acagcttcca
    45721 gtaattgctt ttgcaatttc attacgcctc ctccagggtc ataccggcaa acatctcatc
    45781 catcagggca atcaacccgg cgttgggtcg ggttgtgtaa atcccttgtt gcggatgttc
    45841 tttatcaacg ccacgcagga acagatagat aacgccgcca aagtgacgat catagtcgta
    45901 atcagcaatg cgatggcgca gatagcgatg cagcgccagg gtataaagct gatattgcag
    45961 atcatagcgg tgtgcctgca tcgctgctgc catagcctgt tgggtgtaag ccgaactgtc
    46021 ttcacccagc cagttggatt tatagtcgag caggtaataa cgcccttcgt ggcggaatac
    46081 caggtcgata aagcctttta acatgccgcg tacctgcatg aactccagcg gcgggcagcc
    46141 agcggatagc gggtcaaact ggcgaattag tgcatcaagc tggctggcga tgagcggttc
    46201 gctaatcggc aggtaaaact ccatctccac ctgtttatcg cggtcggaaa gctgactcag
    46261 gctaacaccc gtttcattga gaggtgcctg gaggacagcc gtgatccact cggtcaacac
    46321 cggttcccac tgcgattcaa agccgccgag ttccagtttt tcctgtaccc agttcgggtc
    46381 aaccggctgg gtaaaatcga ggtcttcaaa caaactgtgc aagaatgtcc ccggtgacgc
    46441 accgcgcggg aactgatgcg gtgttaacgt cggttcttca acgacgctga ccacgcctgc
    46501 ggcatcaaca tccagccgtg gcatcagatc ctgagcgata ccgtgaccac gctgctgcaa
    46561 gccggagtag ctggtgacac gccagttatc gccgggcaat cgttgtagtg tcctcgcatt
    46621 cagttctgca gttaaagcat cattaacctg ccagggctgg ttatcaccag tttgtgccgt
    46681 ttgccaggca atatcatcat cgcataacgc ttcaatacag gtgcgaagcc ctgccgcatc
    46741 ttgcggttcc cctttttgca gcaaacgccc gagcgcactt tggtggacgt cggtgtcacc
    46801 ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg agactgcaat gccaaaccga
    46861 acgcgtcagc gccacgtaaa gcaaacgcag atcttccgcc agacgttcga cctccgcgag
    46921 gtcgacgctt tctggcgcag cattaagatc caaaactgcc tcaaacgagt ggcgatcgtg
    46981 ataaaacgcc tgatcctgga cgcggaaatt ggtaataaat ggcaaccaga ccagcggata
    47041 ttccagccct ttcgatttat ggatcgtgac aatctgcacc agatgtttat cactttcgag
    47101 gcgcatttgt tggctggagg cattactgtc tggctcgagg atatgttgcg ataaccagcg
    47161 taccagcgca tgttcacttt ccagctgcgt tccggcttct tgaagcagtt cgctgatatg
    47221 caagatatcg gtaagacgcc gctcaccgcc tgccgttgcc agcaagtttt ctgcaatgtt
    47281 acgcgccgac atcagcgccc gcagcatcgg cataacgcca cgtttgcgcc agatttgccg
    47341 ataaccatcg aactcttcga ctaccgcatc ccacgcatgt tcgtcattat tcagcgtttc
    47401 aatatccagc gcattcagcc ccatcattga cgttgccagc gcactgcgca gggtgttctc
    47461 acgttcgggc gtcatcaccg cctgcaacag ccaaagcatt tcctgcgctt ccagagtttc
    47521 aaaaacacta tcgcggttcg aaaggtaaac ggaagggatt tccagcaacg ttaaggcatc
    47581 gcgcacctgg gcggcctcct ggcggctgcg caccagcacg ctgatgtccg aagcacgcac
    47641 cggacgcgcg tcgtcgccgt tcatcagcaa cgcttcgccc cgctgtccgg cttgtagcca
    47701 gtcgcggatt tgcgcagcac atacctgcgc catggtactt tgatagtcgc caacgccgca
    47761 gctttcgcct tccatcagcc acattttcat cgcaggctgc gtttcacctt taaagacaaa
    47821 acgtaacgcc tgatttttcc cggctgattt cactggaata aacggtattt cgcgaaacat
    47881 gaacgcgtca tcagtctggc tgaaaagctt gttcacactg ttcaccattc caggtgcgga
    47941 gcgccagttg gtgtctaaag tgtagtgggc gtgaacttca ctacgcgcct tcatataagt
    48001 gaagatatcc gcaccccgga atgcatatat ggcctgcttc gggtcgccaa tcagcaacaa
    48061 tgcggtttct ggctgatggt gccagatacg gcgaaaaatt cggtactgct gggggtcggt
    48121 atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta cggatcgccg ctgccaacac
    48181 ctcgccgctt tcgctacgca gcgcggaatc gagccgactt aacatgtcat caaaacccaa
    48241 ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg atctcagcca atgcgcgggt
    48301 gatcaccaga tcacggatcg acaatggttc tgcaagcagt tgatcgatcg cctcaaacag
    48361 tggatgtcgc ggggtttccc ccccggcttt cgtgcgatct tctaagaaac gctgggagaa
    48421 tttttccagc gactccggca actgataact gtttgtctct tcttctgccc aggcgctgat
    48481 cttgtcgatc catttagcct gattgctacg gttaaacttg cgtcgatcaa taccagaaga
    48541 ttcgatcagc gcatccagtt cacccactgc gtcgcgccac tgctgtttta ccgtatcaat
    48601 acgcgccaca atttgcgcgt gacgggaagc tagcgtttca tcatcgggcg gcggtgcttt
    48661 gataaccggc gcttcgcctt gcagataacg attaatatcg cgcagcaacg cctgcggccc
    48721 tttccaggtt tcaaagacga cctgggctat ttcacgcggc agcgggtagc agtggcgacg
    48781 ccagaaatcg gcgcaggcct ggtagcgtag cagagactca tcttcaatca gctgctgctc
    48841 aaacagcatg ccggattcaa aggcattcag gttgagcatg cgctggcaaa agccgtgaat
    48901 agtaaagact gccgcttcat ccatctgccg ttcggctaac aacaaccact gcgcggcttg
    48961 cgctttatcg tcgacctctt ccagcaggtg ttcgtacaat ggattgtcgg tggtttcacg
    49021 cagacaggcg atgcgcaact cgtggatatt gctacggata cgaccgcgca attctgccgt
    49081 ggcagcctcg gtaaaggtga ccaccagcag ttcttcaacg gtcagcgggc ggggaaaggc
    49141 ggcggaaccg cctagtccaa gtaacaggcg caaataaagc gccgcaatcg taaaggtttt
    49201 gcctgtgccg gcagaggctt caatcaggcg ctcgccctgt aagggcaagc gcaaaggatc
    49261 tagtgtctcg gcgacatcac tcattctttt cactcatcag gggcattgtt tgctgcaacg
    49321 cgctgacgtt ctcccacact ttccagcctt caggatgcac atattcggct ttcccgtttt
    49381 ggctgccgga aatctgcgac aggatagcca tgccttgcgg ctcgactacc gcctgatgga
    49441 agaaatcggc cagtttttgc ggcgtcagca gtttaatctg ggccacgatt ttatcacgcg
    49501 aatcgaagcg catattgccg cgatcgaaat ctttacttaa cttcgatgct tcttcgccga
    49561 gcgtttgcgg tgcctgcagc atctgggtaa ttaccgcctg ctggatttgc gcaaactcat
    49621 ctggcttcat cgctcgcaat tttgcctctg cggttgggaa aaacgccttg taacgctccc
    49681 acaagaatga aggctgttta tcattgcttt gcaaaaggaa gcccatgccc cactgacgcc
    49741 ccacgctcat tggaaacgca aacacggcat agcccaattg ttcttcggta cgcaactgat
    49801 tgtagaacca cggctgtacg atctgcccca acagagagct ataggctgag ctggtgtatt
    49861 catcgtagcc agtcggtaca aataccgctg ccagtgcgga gtcggtgctg ttaccggctt
    49921 tttcaaagat gacggattgt tttttatcga ccactacatc tttgtttcga caccactctg
    49981 aaccatcagc gcccaactgt ttttgcacat cgcgtgccag cgttgttgcc tgggcctcgg
    50041 tcatgttgcc gataaccata aactctggtc gagcccctga ttttaaggcg tcgcgataag
    50101 ccagcacctc tttcaacgta atggagggca aaattttacg ccgttcatct cgcgagaagt
    50161 acggcacttg cgagagcatc tgcgcgggca taatcgcctg ctcaaacgct ttaccctttt
    50221 ctgcggaatc catcatctgg ttataccagg acttcgcctg ctcaagctga tcttccgtag
    50281 cggtatagct aaagtacccc tcgagcaatg cctggaacag ctgcggcaga cgctgggtgt
    50341 aaccattagc attaaccata aggccgttgt tagcgttggt ggaaaaactt atgccaccaa
    50401 ccgacgcctg gttgcttaac tgatcaagcg ccagccctgc gagataatca ttgagcgcaa
    50461 acatcacctg attgcgggcg ctgtccatgg ctttcggatt acgcaaaatc aggctgacat
    50521 cagctttggg ttcgctggaa aaataacggc ttggcgcata caccacgcgc agattcgact
    50581 catcaacaat cagctctgga tggtcatatt tcttctctga cttaatcagc gagaaatcgt
    50641 caggaatata ggggttaagc tccggtaagg agagcgcaat attggcagct ttttgctgcc
    50701 agtcagcgaa agtttgttcg ctgattttat cgacctgata cggcgcatcg acaaaataag
    50761 ccgttttatt gtgcggctct ttcgggctga tataccagat acgcgcattc tgcggcgtca
    50821 tcatcgccag acgttctttt actgctttag catcgtaccg atcggcaata ttgactgcat
    50881 ccagcgtatg ctcaaccgga acgcgaatca tggtatctgc cagccattcg acgtaatcca
    50941 tatcacgggt gatcgacgga taacggaagt cgatatccag cacattcgcc agttcatcga
    51001 agtattgttt atcaatgcct ttttcacgta acagattgag atagctaaaa attgccgcca
    51061 caacctgatc gcgattagcc aggcctttat cggttaaaga cgcagagatc gctaatacgc
    51121 cgctgttgcc gttgacgata ggatcggagt tggcgctaat gccctcaact aatccctgct
    51181 tttgcagcca gtcggaaagt gtacctgggc tgcgattacc aatcagatag gtaatcaatt
    51241 catccgtttt actacggaac ttcgctgagt tgttatcgat gcgaaactca acgcgcagaa
    51301 ctttacgcgg cattgccggg acgtaatgaa tgataatgcc cttttgcgcg tcggtgacta
    51361 ccggcacggt gatttccggt tttttgctct ctttgttcgg cacgcgacca aaggtgtccg
    51421 ccgccatttt tgccaactcc ggcagcggtt tattgctgta aataaccgcc ttcatcagat
    51481 tggcggaata gtacttctcg tggaaatctt tcagcgcctg ctgcaccgga ttaccaggtt
    51541 tatcgcttaa agtttcgagg ttaccaccag aaaactttga accggggtgt gccgggttaa
    51601 tggtttctgc gctgacctgt gccatgcgca tcccgtcacg cgtacgcgcc atggttaatt
    51661 cagcgttcac cgcattacgc tcacgttcgg catatttctt gtcgagcaaa ggttcagcaa
    51721 tagcatcggc caggcggtct accgcaccag gcaaggcgtc gttctcaact tccagataga
    51781 aagccgtgcg atacggcgca gtgctggcat tgtgactacc gccgtgcatt ttgagatatt
    51841 cggccagact gtcagcctgc gggtactttt tcgaccccat cagactcata tgttcaaggt
    51901 aatgtgccag cccctggtac gcctcgggat cttccagcga cccaacgggc accaccagcg
    51961 ccgagagcga tttaactgcc tgcggatcag aaaccagcaa gaccaccata ccgttatcca
    52021 gacgtatagc ctgatactgg cggttatctt tatcactttt acggatggtt tcctgaatcg
    52081 gctgccatcc cgtttctgcc tgacttaagg gtgcccaaag ggcaactaac aacaataatg
    52141 ctttgaacca ggtgctgcgg ggcattctcg gacctcataa tcaacttaat tttctgtcca
    52201 gattcaacac gttaacgcaa atcatctgcc agaatttaat cttgtgctgc acgagtcagc
    52261 ctatgtttat ataaccatca gtccgtgact ggtgcgcatc ataaagtaag cggatagatt
    52321 gcgcaatttt tatacagcac tcatgactga ttaaagcgaa acagcggtaa caggaaacgt
    52381 tgcgactgtt caacgatggc ctccattgtc tctggtgtta attgccgcca gagcctttga
    52441 taccagatat catcaccttc gccacgcacc atcatgttac cttcgtaagc ctgaaggaat
    52501 ttcgtacggg ctttttgcaa cgtggaatcg tcatccagca tggcatcgtt ttgcgcgtca
    52561 taacaggttt ttagccacgc gccgccactt tcaggtaaca ccagcaatgg cgcggacatc
    52621 ccttcacgat acccctcaat cagttgtgag aggtaatgca aagcctgttc ggctgcaagc
    52681 ggcggaaaac gccactcgcc gtctttgcgt agaaaaaggc gactttcacc attaccaccg
    52741 ctggcacagt agacaaggtg ttccagccaa agttgcattc cctgcgccac acttaataaa
    52801 gagggacgcc agcgcaacag gccatccggc tgcacctgcg gcaaccagcc agttatctgc
    52861 acaccgttgc aggcgagatc aatttccata ctctgccccg gctggcgaca ggcaatgact
    52921 ctgtcggcaa gctgctgcat ctcctggcac tgagtttccc agaaaatttc accaaaagcg
    52981 ccatacggta aatcccctgc cgcccggaag cgacggaaca agcgttcggc atcatcctgc
    53041 tcaaccagtg cattcaataa ctgctgattg atttgataac ggctaagccc ttccagaata
    53101 aatggctcgg tgtcggggat ttcgctgtct tcagtacgga agttcacctg caaacgcatc
    53161 tggaagaacg cccgcaccgg atgtgcccag aatcgttgta gcgtttccag cggcacggtt
    53221 tccggtaagg taaacggcag cggctgaaca aattcagaat gtgctttacc agcctggctg
    53281 gccgcaggta gccattcacg agcatagctt tgtcgttcgc ctggctgata gttttgtgga
    53341 tcaaacggca ttcgggtatg gaggcaagta agatgcgctt ttacccttgc ctcgctttca
    53401 tcacagttga gcgcttcatc gcccggtaga taatgacttt gcccgatgta gtcgatcagt
    53461 tcctgcacca gtaccgacgg gaaacgttca ctgttatcct gaatggaacg cccgatatag
    53521 ctgatataga gtttttgctg cgcggaaatt aacgcttcca ggaacagata gcggtcgtca
    53581 tcgcgacggc tacggtcgcc acgcttcggt ttctggctca tcaggtcaaa gcccaatggc
    53641 gcaagctgac gtggataaac gccgtcgttc attcccagca ggcaaaccac tttgaacgga
    53701 attgaacgca ttggcatcag agtacaaatg ttaaccggtc cggcgagaaa acgctggctg
    53761 atacgttctt gatccagacg ctgtgccagt tcatcacgca atagtgacag cggcaccgcg
    53821 tcgccatact gcgcacctaa accttcggcg ataatcgcct gccattgttg ttcgatcagc
    53881 gtcatcgccg cttcggtttc cgcatccggc aggaagaagg cgctgagcat atcgcgacaa
    53941 accggcaacc actcttctag cggacgctcc tgtgccagcc cgcgacgcca gatgtttagc
    54001 tgcattagca gtgaagccag atgccccacc agttctgcaa ttaagccgct cgattcatca
    54061 taaggtagaa ccgattgcca ctcgccctgc gcgctctcca tcgcgtagcc cagcaacata
    54121 cgcgtcaggc caaatcgcca ggtgtgttgt ccggtggcgg ggagttccag ctcgcgaacg
    54181 ttgtcgtcat ctatccccca acgaatgccg gattcgttga cccactggcg taaataacgc
    54241 agcccttctt cggtgatgtc aaaccgcgcc gccagcaccg gcacatccag taatgccagc
    54301 acgtcttccg acacaaagcg gctgtctggc aacgataaca ggctgataaa cgcttccagt
    54361 accggatgcg actgccgcgc ccgacggtcg gaaatggcgt aaggcaggta acgatccgca
    54421 ggtgcgctac caaacacagc ccgaataaac ggactgtagc tgtcgatatc agccaccatc
    54481 acgatgatgt cgcgcggagt aagtgtcggg tcttcttcca gcatcgccag caggcgatcg
    54541 tgcaaaactt caacttcacg ctgcgggcta tggcaaacgt ggaaggtgat actgctatcc
    54601 agtggatcaa gcgggcgttt gttatcgcta cgggaaaact cttcgatgtt cacaccagca
    54661 acggcacggt tttccagttc cagaatgtca gactgaatgt tatgcagcag gttatctggc
    54721 gtgacatcga caaaggcgtc cagctcctgg ctgctctcca ggtcagaaag gagataaatg
    54781 tagtcgcgcc caagcttacc ccatgaagcc agcagcgggt tgccgacatc ctgttcacca
    54841 tcgctgttaa agagctgccc ggcattttcg ctatcacgaa atagcggcaa ttcgcgatct
    54901 tcaaaactgt gtcgacgctg acgggtcagc agtttcgcca gataagcagg atctttaata
    54961 tcgccccagt aataacggca ggggttggta aacaggagat ggatttcaat atgtttaccc
    55021 agcgcctgta gcgcctggag ataaacaggc ggtaacgcgg aaataccgca tataaagacg
    55081 cgcgaaggta accccggcgg gcaggtcgtc gcggactcca gcgtttcgat aaagcgctga
    55141 tagagattgg cgcggtgcca gcgcggttgc ccgagttgat gggtatattc caccagcgcc
    55201 ttccacaacg gggcttgcca ggcctgtgct tctcccagcc cttcaaccaa atgtcctgtt
    55261 tcccactgtg ccagccagtc cggacgatag accagatact ggtcaaacag gtccgccgct
    55321 tttgaggaaa gctggaacag ttttcgcttg tcgctatcgt cagtcagata atgccgcaac
    55381 agggtaaagt cttcgcgctc cagcaattgc ggcagcagag tcatcagttt ccagctcatg
    55441 ctctgtttgt taaaggcgct ctctttgggg atttccggta acacccggac gaacatatcc
    55501 cagataaagc tcgctggcag cggaaaatca atgtttgccg caatactaaa cttttgcgac
    55561 agggtcattt gcagccactg cgccataccg gtactttgca ccagaatcat ctctggttcg
    55621 aaaggatcgt ccagccgttc gcgttcgaca ataaactcca tcaacgcttc cagcacgtcc
    55681 agacgattgg aatggtagac ccttaacata gcggctcctg actactgacg attcgggcaa
    55741 tgcaggcggg tcatctcgcc ttctctgccc ccgggtgaaa ctagcgtaac gctgatgctg
    55801 acacatcccg cctgcgatgt ctgcattcgg ttgacctgcc agttggcagg tggcgaaatc
    55861 gcgcgcagtt gcgtttgctg ccaaccatgc cgccagagct gttggtactg gtttctgctg
    55921 gcgagactgt tcattaatgt tcgctggata cccgataacg cagtgacaat catcaccatc
    55981 agcaccatcg caaccattac ctccggcagg ctaaagcctt gttgattctt cagggaagct
    56041 gacataacgc cctctctttc agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa
    56101 tgtttccatc aatgacttcg cctgttcgcc acaacgaaac gccttcataa ccggcaacca
    56161 ataaggcttc attatctgcc agtaaacgca aacaaacccg ggcatcggtt ccagcgtact
    56221 gcgagcactg aactgcgggc tgcgtctgcc aggagtgcat tttcccccac gccagcgccg
    56281 actgaacgat ggcctggcgg cgcaatgact gactttctat gctcacgcga gaggcaaaac
    56341 tgcgatcctg ctgactcatt ccttgcaata gcaagctacc caaaaccagc agcatcagga
    56401 ccagagccag tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg tcacgctata
    56461 gctggcatcc accacggttt gcggttcaga cttactggca gcacgcatat taaccgtcaa
    56521 caccggcgag aagccgctga catcctgacg tacgacctga aaagtgtcga taatgatggc
    56581 atccggatta gtgactttat cccagccctt accttcacag gatgtcgcac cgcgtagcgt
    56641 ttccagcaca tgctccttca gacgaaatcc aatctggtca gactctttta ccggttcgcg
    56701 atcccagata ccgttactgt tcgcatccca ctgcacaatg atacagtcac cctgtccgac
    56761 aatttccagc ccttcgccgg tacagctgcc atgacaataa cccgccctct ggagatgctt
    56821 cgcgacggta aatacccgca gccagatttc atcttccagc gccagcttac gggtgctcgt
    56881 taaactttca cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca atacgctact
    56941 gatcgccata gcaatcaaca cttccagcag agaaaaacct tgctctttta caggcatcct
    57001 tctgtttctc cttgctgaca aagccggagt cttccccacg gcgaaaccac cagccaccac
    57061 tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg cggtattgcg caggccaaag
    57121 aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc
    57181 aatggtgaac tgccatgaca ggtattggcc ccagcagcgg aactcacaag gcaccataac
    57241 gtcccctccc tgataacgct gatactgtgg tcgcggttat gccagttggc atcttcacgt
    57301 aaatagagca aatagtcccg cgcctggctg gcggtttgcc atagccgttg cgactgctgc
    57361 cagtattgcc agccatagag tccacttgcg cttagcatga ccaaaatcag catcgcgacc
    57421 agcgtttcaa tcagcgtata accacgttgt gttttcatgc cggcagtatg gagcgaggag
    57481 aaaaaaagac gagggccagt ttctatttct tcggcgcatc ttccggacta tttacgccgt
    57541 tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag ggtaaaaaaa
    57601 accgacgcac actggcgtcg gctctggcag gatgtttcgt aattagatag ccaccggcgc
    57661 tttaatgccc ggatgtggat cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa
    57721 gatggattcg ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa
    57781 ttgcagatga gtttgatcca tatgattgct gtacaggtgc gtatcgccac cggtccagac
    57841 aaaatcaccc acttccagat cgcactgctg cgccatcata tgcaccagta acgcgtagct
    57901 ggcaatgttg aacggcaggc cgaggaagac gtcacaggag cgctgataaa gctggcaaga
    57961 gagtttgccg tctgccacat agaactggaa gaatgcatgg cacggtgcca gcgccatttt
    58021 atccagttcg cctacgttcc acgctgaaac aataatgcgg cgggaatccg ggtcgttttt
    58081 cagctggttc agtaccgtag tgatctggtc aatatgacga ccatctggcg ttggccaggc
    58141 gcgccactgt ttaccataca ctggtccgag gtcgccgttt tcatcggccc attcgtccca
    58201 gatggtgaca ttgttttcgt gtagataagc aatgttagtg tcgccctgca gaaaccacag
    58261 cagttcgtgg atgatggaac gcaggtggca acgtttagtt gtcaccagcg ggaatccatc
    58321 ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc
    58381 gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt
    58441 tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc
    58501 cgcgacaatc atcgggatgg aaagaatttg ccccatgctg atgtactgca cccaggcacc
    58561 ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc
    58621 aatcaggaac aagccagaga cagctcccat tgggcgcggt ttacgaatat acaggttgag
    58681 gataataaac agcaccacac cttccagcag cagctcgtaa agctgtgatg ggtggcgcgg
    58741 cagcacaccg taagtgtcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat
    58801 atcttctgta cgggagcctg ggaacagcat ggcaaacggg aagttcgggt caacgcggcc
    58861 ccacaattca ccgttaataa agttgcccag acgcccggca ccaagaccaa acggaatgag
    58921 tggtgcgata aaatcagaaa cctggaagaa ggaacgttta gtacggcggg caaagataat
    58981 catcaccacg ataacgccaa tcaggccgcc gtggaaagac atgccgccgt cccagacacg
    59041 gaacagatac agcggatcgg ccataaactg cgggaaattg tagaacagaa cataaccaat
    59101 acgtcccccg aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt
    59161 ggtccagccg ctgcccggac gattcgcccg tcgtgttgcc agccacattg caaaaatgaa
    59221 acccaccaga tacatcaggc cgtaccagtg aagcgccacg ggtcctattg agaaaatgac
    59281 cggatcaaac tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt
    59341 atttcgctga aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg
    59401 tgcgaataat agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata
    59461 tgatccgcgc tataaccctc cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc
    59521 tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt
    59581 ttctgcttcg gcataatcaa tgcgccgcag caggtatttt acccgcgcta cagaacgtcc
    59641 gttcatagac agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc
    59701 cgccatttca ccgcacaaac ggagatcgat tccatgtatt tccgcttccc gggcgatcat
    59761 cgccagagct cgtaacattg caggatgaag actgtcataa atgttcgcca cccgggtatt
    59821 gttgcgatca acggccagaa tgtattgagt cagatcgttg gtgccaacag agatgaaatc
    59881 gacccgcttt gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc
    59941 gatacgtggt ttgggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc
    60001 aatcaggcgg cgtgcttcat caacttcatc gaggcttgtg accatcggca acagaatatt
    60061 caggttgccc gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc
    60121 cggctgatcg agcgtaatgc gaatcccacg ccaacccagg catggattct cttcgctgat
    60181 cggcatgtaa ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt
    60241 atcattgaac atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacgggaa
    60301 accactttgc agcatgaatg ggatttcagt gcgataaaga ccgatgccat caatacggct
    60361 gcccagtttt tcttcatgtt ccgggcttaa accagcattg agcatgactt ttatacgctc
    60421 accgcttttt aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat
    60481 ctcttcacta attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa
    60541 ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc agcaccgaag gctgaatatc
    60601 cgcgcccatc acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc
    60661 tgcgccatct cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagagttgt
    60721 cgctgacagt tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt
    60781 agcgtcatca agatgaaaca gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc
    60841 tttgagatag ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt
    60901 taccgcccac tctgccaccg agcctttatc aacctcggca aacaattcgc gacgcagccg
    60961 ggtgtccgaa agtaggtgcg agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc
    61021 ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt
    61081 cagtcgttcg cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa
    61141 aggtaacgtg gcatcctgcc agccttcggc aatcgccaca ccaggtgctg ccggtaatgc
    61201 gcggattcgc gtctggcgat attgcccaaa caaggcagtc aactgcgact gagaaagaat
    61261 agctgccatc tgggtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg
    61321 caactctcgt tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac
    61381 gcctaaaaac gcgcggaaac gttcttcttt tacggagggg atgtatttga agctggggtg
    61441 cttttgcgca tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc
    61501 ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat
    61561 caggtagtaa caacgtcgat catgatcggc caggtagacc gaacagacct cggtatccat
    61621 cgcaagacag atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc
    61681 taccttttcg actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt
    61741 acgtcgataa gcagatgtgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac
    61801 actcgcgaac tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct
    61861 gaccggatac cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg
    61921 catattgatt tctgcatcgc cgctcaccag ctgcaagaga aaccattttt gtttttggcc
    61981 gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca
    62041 gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa
    62101 ttcacggtac atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg
    62161 ccaggagtgc tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac
    62221 gatacctacg tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc
    62281 tttatatacg aatgattgtt tcatactcca agaagacggt aaaccactct ctgcagggca
    62341 ttacacacta ataacaattg aataactcac agttatgtgc agagttataa acagaggaag
    62401 gggtggatag ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat
    62461 tactacggga aaacccggag cattctgaat aagcccgata tagacatttt aaaatattca
    62521 ttattagtca atgcattaca tgtaatttga gtaatgaaaa agagtaattc gtgacccagg
    62581 tcacacctct catttacggg ttggcgaaag atcaaccaat gccgtattta tccacagaat
    62641 gtgccactaa gttaagcact gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg
    62701 taaatggaaa cagtagtgga ggtttttcac agttatccca gctttctgtg gataacatgg
    62761 tgtaagatcc tgtttatttt cagtgaccag atttggaaaa cctattgcag tgttgcgcaa
    62821 ctcgattacc ggcagcctaa aaagcaatat aaatcagaga attgaacaac acatgtggaa
    62881 aaagttacac tgcgaatatt cggcacataa ttgctgtttg ttttttaacc aaggtatcat
    62941 gacatgtccc aacctcgccc actcctctct cctcccgaaa ctgaagaaca gttgttagcg
    63001 caagcacagc aactttctgg ttatacattg ggagaactgg cggcacttgc cgggctggtt
    63061 acgccagaga atttaaaacg cgataagggc tggattggcg tgttactgga gatctggcta
    63121 ggtgccagcg cagggagtaa acctgagcaa gattttgctg ctctgggcgt ggaacttaaa
    63181 actatccctg tggatagtct tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg
    63241 ttaacgggta atagcggggt gacctgggaa accagccatg tgcgccacaa gctcaaacgc
    63301 gtactgtgga taccggttga aggtgagcgc agcatcccgc tggcgcagcg tcgcgtagga
    63361 tcaccgttgc tgtggagccc gaatgaagag gaagaccgac agctacgcga agactgggaa
    63421 gaattaatgg atatgattgt tctcggtcag gttgagcgga tcaccgctcg tcacggggag
    63481 tatttacaga tacgaccgaa agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc
    63541 cggggcgaac ggattctgac gctgccacgc ggcttttatt tgaagaagaa tttcaccagt
    63601 gcactactgg cccgtcattt tctgatccag tagccatcgc tttgacctgc cgctttccgg
    63661 gcatataatt accgcttcat ttttttggca gggcttttta gatgttattt gcatggataa
    63721 ccgatcctaa cgcctggctt gcgctcggta cgctgacgct gctggagatc gttcttggga
    63781 tcgacaatat tattttcctt tctctggtgg tggcaaagct ccccacagca caacgtgctc
    63841 atgcgcgccg tctggggttg gcgggagcca tggttatgcg tctggcgctg ctggcatcaa
    63901 tcgcctgggt tacgcgcctg acaaatccgc tttttacaat attcagtcag gaaatttccg
    63961 cccgtgattt gattctgctt ctgggtggct tgttccttat ctggaaagcc agcaaggaaa
    64021 tccacgaatc cattgaaggt gaagaagaag ggctgaaaac acgcgtttca tcattcctcg
    64081 gcgctatcgt gcagattatg ctgctggata ttatcttcag cctcgactcg gtgattaccg
    64141 ctgtgggtct gtcagatcac ctgtttatta tgatggccgc cgtggtgatt gccgtaggcg
    64201 tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga acgccatcct tcggtaaaaa
    64261 tgctggcgct ctctttcctg attctggtcg gctttaccct gattctggaa agtttcgaca
    64321 tccacgtacc gaaaggttac atctacttcg cgatgttctt ctctattgcg gt
//
LOCUS       DAJTVJ010000005        63004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000005 DAJTVJ010000000
VERSION     DAJTVJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.005"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..414)
                     /locus_tag="OO448_000293"
                     /pseudo
     CDS             complement(1..414)
                     /locus_tag="OO448_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(520..3081)
                     /gene="mutS"
                     /locus_tag="OO448_000294"
     CDS             complement(520..3081)
                     /gene="mutS"
                     /locus_tag="OO448_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6335618.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3157..3291
                     /locus_tag="OO448_000295"
     CDS             3157..3291
                     /locus_tag="OO448_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335619.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3368..3721
                     /locus_tag="OO448_000296"
     CDS             3368..3721
                     /locus_tag="OO448_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6335620.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3758..5835)
                     /gene="flhA"
                     /locus_tag="OO448_000297"
                     /pseudo
     CDS             complement(3758..5835)
                     /gene="flhA"
                     /locus_tag="OO448_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5909..6919)
                     /gene="hypE"
                     /locus_tag="OO448_000298"
     CDS             complement(5909..6919)
                     /gene="hypE"
                     /locus_tag="OO448_000298"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6335621.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6916..8037)
                     /gene="hypD"
                     /locus_tag="OO448_000299"
     CDS             complement(6916..8037)
                     /gene="hypD"
                     /locus_tag="OO448_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6335622.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8037..8309)
                     /gene="hypC"
                     /locus_tag="OO448_000300"
     CDS             complement(8037..8309)
                     /gene="hypC"
                     /locus_tag="OO448_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6335623.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8300..9172)
                     /gene="hypB"
                     /locus_tag="OO448_000301"
     CDS             complement(8300..9172)
                     /gene="hypB"
                     /locus_tag="OO448_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6335624.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9176..9526)
                     /gene="hypA"
                     /locus_tag="OO448_000302"
     CDS             complement(9176..9526)
                     /gene="hypA"
                     /locus_tag="OO448_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6335625.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9738..10199
                     /gene="hycA"
                     /locus_tag="OO448_000303"
     CDS             9738..10199
                     /gene="hycA"
                     /locus_tag="OO448_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6335626.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10324..10935
                     /gene="hycB"
                     /locus_tag="OO448_000304"
     CDS             10324..10935
                     /gene="hycB"
                     /locus_tag="OO448_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6335627.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10932..12758
                     /gene="hycC"
                     /locus_tag="OO448_000305"
     CDS             10932..12758
                     /gene="hycC"
                     /locus_tag="OO448_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6335628.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12761..13684
                     /gene="hycD"
                     /locus_tag="OO448_000306"
     CDS             12761..13684
                     /gene="hycD"
                     /locus_tag="OO448_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6335629.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13702..15399
                     /gene="hycE"
                     /locus_tag="OO448_000307"
     CDS             13702..15399
                     /gene="hycE"
                     /locus_tag="OO448_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6335630.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15409..15951
                     /gene="hycF"
                     /locus_tag="OO448_000308"
     CDS             15409..15951
                     /gene="hycF"
                     /locus_tag="OO448_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6335631.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15951..16718
                     /gene="hycG"
                     /locus_tag="OO448_000309"
     CDS             15951..16718
                     /gene="hycG"
                     /locus_tag="OO448_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6335632.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16715..17125
                     /gene="hycH"
                     /locus_tag="OO448_000310"
     CDS             16715..17125
                     /gene="hycH"
                     /locus_tag="OO448_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6335633.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17118..17588
                     /gene="hycI"
                     /locus_tag="OO448_000311"
     CDS             17118..17588
                     /gene="hycI"
                     /locus_tag="OO448_000311"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6335634.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17613..18386
                     /locus_tag="OO448_000312"
     CDS             17613..18386
                     /locus_tag="OO448_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335635.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWRFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18548..18859
                     /locus_tag="OO448_000313"
     CDS             18548..18859
                     /locus_tag="OO448_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6335636.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18856..19275
                     /locus_tag="OO448_000314"
     CDS             18856..19275
                     /locus_tag="OO448_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6335637.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19389..20813)
                     /gene="ascB"
                     /locus_tag="OO448_000315"
     CDS             complement(19389..20813)
                     /gene="ascB"
                     /locus_tag="OO448_000315"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6335638.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20822..22279)
                     /gene="ascF"
                     /locus_tag="OO448_000316"
     CDS             complement(20822..22279)
                     /gene="ascF"
                     /locus_tag="OO448_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6335639.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22539..23549
                     /gene="ascG"
                     /locus_tag="OO448_000317"
     CDS             22539..23549
                     /gene="ascG"
                     /locus_tag="OO448_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6335640.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIT
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23697..24224
                     /gene="hydN"
                     /locus_tag="OO448_000318"
     CDS             23697..24224
                     /gene="hydN"
                     /locus_tag="OO448_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6335641.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24377..26629
                     /gene="hypF"
                     /locus_tag="OO448_000319"
     CDS             24377..26629
                     /gene="hypF"
                     /locus_tag="OO448_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6335642.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26757..27890)
                     /gene="norW"
                     /locus_tag="OO448_000320"
     CDS             complement(26757..27890)
                     /gene="norW"
                     /locus_tag="OO448_000320"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6335643.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27887..29326)
                     /gene="norV"
                     /locus_tag="OO448_000321"
                     /pseudo
     CDS             complement(27887..29326)
                     /gene="norV"
                     /locus_tag="OO448_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
     gene            29513..31027
                     /gene="norR"
                     /locus_tag="OO448_000322"
     CDS             29513..31027
                     /gene="norR"
                     /locus_tag="OO448_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6335644.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31024..31989)
                     /gene="gutQ"
                     /locus_tag="OO448_000323"
     CDS             complement(31024..31989)
                     /gene="gutQ"
                     /locus_tag="OO448_000323"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6335645.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31982..32755)
                     /gene="srlR"
                     /locus_tag="OO448_000324"
                     /gene_synonym="gutR"
     CDS             complement(31982..32755)
                     /gene="srlR"
                     /locus_tag="OO448_000324"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6335646.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32822..33181)
                     /gene="gutM"
                     /locus_tag="OO448_000325"
     CDS             complement(32822..33181)
                     /gene="gutM"
                     /locus_tag="OO448_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6335647.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33287..34055)
                     /gene="srlD"
                     /locus_tag="OO448_000326"
                     /pseudo
     CDS             complement(33287..34055)
                     /gene="srlD"
                     /locus_tag="OO448_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34059..34430)
                     /gene="srlB"
                     /locus_tag="OO448_000327"
                     /gene_synonym="gutB"
     CDS             complement(34059..34430)
                     /gene="srlB"
                     /locus_tag="OO448_000327"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6335648.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34441..35400)
                     /gene="srlE"
                     /locus_tag="OO448_000328"
                     /gene_synonym="gutE"
     CDS             complement(34441..35400)
                     /gene="srlE"
                     /locus_tag="OO448_000328"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6335649.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35397..35960)
                     /gene="srlA"
                     /locus_tag="OO448_000329"
                     /gene_synonym="gutA"
     CDS             complement(35397..35960)
                     /gene="srlA"
                     /locus_tag="OO448_000329"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6335650.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36216..37301
                     /gene="mltB"
                     /locus_tag="OO448_000330"
     CDS             36216..37301
                     /gene="mltB"
                     /locus_tag="OO448_000330"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6335651.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37446..37943
                     /gene="pncC"
                     /locus_tag="OO448_000331"
     CDS             37446..37943
                     /gene="pncC"
                     /locus_tag="OO448_000331"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6335652.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38023..39084
                     /gene="recA"
                     /locus_tag="OO448_000332"
     CDS             38023..39084
                     /gene="recA"
                     /locus_tag="OO448_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6335653.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39312..39812
                     /gene="recX"
                     /locus_tag="OO448_000333"
     CDS             39312..39812
                     /gene="recX"
                     /locus_tag="OO448_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6335654.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39940..42570
                     /gene="alaS"
                     /locus_tag="OO448_000334"
     CDS             39940..42570
                     /gene="alaS"
                     /locus_tag="OO448_000334"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6335655.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42805..42990
                     /gene="csrA"
                     /locus_tag="OO448_000335"
     CDS             42805..42990
                     /gene="csrA"
                     /locus_tag="OO448_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6335656.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43306..43398
                     /locus_tag="OO448_000336"
     tRNA            43306..43398
                     /locus_tag="OO448_000336"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43340..43342,aa:Ser,seq:gct)
     gene            43402..43478
                     /locus_tag="OO448_000337"
     tRNA            43402..43478
                     /locus_tag="OO448_000337"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43436..43438,aa:Arg,seq:acg)
     gene            43542..43618
                     /locus_tag="OO448_000338"
     tRNA            43542..43618
                     /locus_tag="OO448_000338"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43576..43578,aa:Arg,seq:acg)
     gene            43817..43893
                     /locus_tag="OO448_000339"
     tRNA            43817..43893
                     /locus_tag="OO448_000339"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43851..43853,aa:Arg,seq:acg)
     gene            44174..44740
                     /gene="yqaB"
                     /locus_tag="OO448_000340"
     CDS             44174..44740
                     /gene="yqaB"
                     /locus_tag="OO448_000340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6335657.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44737..45165
                     /locus_tag="OO448_000341"
     CDS             44737..45165
                     /locus_tag="OO448_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6335658.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45238..46794
                     /gene="gshA"
                     /locus_tag="OO448_000342"
     CDS             45238..46794
                     /gene="gshA"
                     /locus_tag="OO448_000342"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6335659.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            46944..47459
                     /gene="luxS"
                     /locus_tag="OO448_000343"
     CDS             46944..47459
                     /gene="luxS"
                     /locus_tag="OO448_000343"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6335660.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47523..49061)
                     /gene="emrB"
                     /locus_tag="OO448_000344"
     CDS             complement(47523..49061)
                     /gene="emrB"
                     /locus_tag="OO448_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6335661.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49078..50250)
                     /gene="emrA"
                     /locus_tag="OO448_000345"
     CDS             complement(49078..50250)
                     /gene="emrA"
                     /locus_tag="OO448_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR6335662.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50377..50907)
                     /gene="emrR"
                     /locus_tag="OO448_000346"
     CDS             complement(50377..50907)
                     /gene="emrR"
                     /locus_tag="OO448_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6335663.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(50998..51333)
                     /gene="ygaH"
                     /locus_tag="OO448_000347"
     CDS             complement(50998..51333)
                     /gene="ygaH"
                     /locus_tag="OO448_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6335664.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51323..52060)
                     /gene="ygaZ"
                     /locus_tag="OO448_000348"
     CDS             complement(51323..52060)
                     /gene="ygaZ"
                     /locus_tag="OO448_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6335665.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52184..53368)
                     /locus_tag="OO448_000349"
     CDS             complement(52184..53368)
                     /locus_tag="OO448_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6335666.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53659..54651)
                     /gene="proX"
                     /locus_tag="OO448_000350"
     CDS             complement(53659..54651)
                     /gene="proX"
                     /locus_tag="OO448_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6335667.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54709..55773)
                     /gene="proW"
                     /locus_tag="OO448_000351"
     CDS             complement(54709..55773)
                     /gene="proW"
                     /locus_tag="OO448_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6335668.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55766..56968)
                     /gene="proV"
                     /locus_tag="OO448_000352"
     CDS             complement(55766..56968)
                     /gene="proV"
                     /locus_tag="OO448_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6335669.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57324..58283)
                     /gene="nrdF"
                     /locus_tag="OO448_000353"
     CDS             complement(57324..58283)
                     /gene="nrdF"
                     /locus_tag="OO448_000353"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6335670.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58293..60437)
                     /gene="nrdE"
                     /locus_tag="OO448_000354"
     CDS             complement(58293..60437)
                     /gene="nrdE"
                     /locus_tag="OO448_000354"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6335671.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60410..60820)
                     /gene="nrdI"
                     /locus_tag="OO448_000355"
                     /pseudo
     CDS             complement(60410..60820)
                     /gene="nrdI"
                     /locus_tag="OO448_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60817..61062)
                     /gene="nrdH"
                     /locus_tag="OO448_000356"
     CDS             complement(60817..61062)
                     /gene="nrdH"
                     /locus_tag="OO448_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6335672.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61310..61639)
                     /locus_tag="OO448_000357"
     CDS             complement(61310..61639)
                     /locus_tag="OO448_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6335673.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61791..62135
                     /locus_tag="OO448_000358"
     CDS             61791..62135
                     /locus_tag="OO448_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6335674.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62172..62621)
                     /gene="alaE"
                     /locus_tag="OO448_000359"
     CDS             complement(62172..62621)
                     /gene="alaE"
                     /locus_tag="OO448_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6335675.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14827 a        16244 c        16982 g        14951 t
ORIGIN      
        1 gatggcatat tttatgttgt cattaatgat ttcaataata tgtggaaggt gatggaattt
       61 cagcaacaga tctattgcct cttgttgctc tgaatcattt aaatcgaaaa accagtcacc
      121 accgctggca agccacatat ttccatcgcc agtttcgaat gcatccaacg ccatcgcttc
      181 gtggttgcct ttaaccgacg taaaccaggg ttggtttagc aggcgcagca cgttaagact
      241 cttcggccca cgatcaatat tatcgcctgt ggaaataagt aagtcggttt cagggtaaaa
      301 agagagttga tgttaacggg attgtaataa ctgatattca ccatgaatat caccaacgac
      361 ccatatatgg cgatagtgat gggcattgat tttttgatag cgtgtagatg gcatggtttt
      421 accctgtaaa ataagctttc ctattataca gggtattttt atttgattcg tcagttgtcg
      481 ttaatattcc cgatagcaaa agactatcgg gaattgttat tacaccagac tcttcaagcg
      541 ataaatccac tccagcgcct gacgcggagt cagtgaatcc gggtcgagat tttccagtgc
      601 ttcgaccgca ggcgaagttt cttctggcac tgacagcaaa gacatctgcg taccatccac
      661 ttgcgtcgcg gcggcgttcg gcgaaatgct ttccagctca cgcagttttt gccgtgcgcg
      721 cttaataacc tctttcggta cgcccgccag agcagcaacc gccagaccgt agcttttgct
      781 cgccgcgccg tcctgcacgc tgtgcataaa ggcgatagtg tcgccgtgct ccagcgcatc
      841 gagatgtacg ttggcgacgc cttccatttt ctccggtagc tgggtcagct cgaaatagtg
      901 ggtggcaaac agcgtcaatg ccttaatctt attcgccaga ttttctgcgc acgcccacgc
      961 cagcgacaga ccatcgtagg tggacgttcc acgcccgatc tcatccatta acaccagact
     1021 gtattcggtg gcgttatgta aaatattggc ggtttcagtc atctccacca taaaggttga
     1081 gcgcccggaa gccaggtcat ctgccgcgcc tacgcgggta aagatgcgat cgataggtcc
     1141 aatctcgact ttttgtgccg gtacatagct gccgatgtag gccatcagcg caatcagtgc
     1201 ggtctggcgc atataggtac ttttaccgcc catgttcgga ccggtgatga tcaacatacg
     1261 gcgctgcggc gacagattca gcgggttagc gataaatggc tcattcagta cttgttcaac
     1321 taccggatgg cgaccttcgg taatgcgaat gcccggttta tcaatgaagg tcgggcaggt
     1381 gtagttcagg gtataggccc gttccgccag gttcaccagc acgtcgagtt ccgccagcgc
     1441 gctcgcgctc tgttgcaacg cttccagatg cggcaacagc aggtcgaaca actcttcata
     1501 aagctgtttt tccagtgcca gtgctttgcc ttttgaggtg agaactttat cttcgtactc
     1561 ttttagctct ggaatgatgt agcgctcggc gtttttcagc gtctggcgac gcatgtagtt
     1621 gatgggtgcc agatggcttt gcccacggct gatttgaatg tagtagccgt gcaccgcatt
     1681 aaagccaact ttcagcgtgt ccaggccggt acgttcacgc tcgcggactt ccagacgctc
     1741 cagataatcg gtcgcgccgt cagccagcgc gcgccactca tccagctctt cgttatagcc
     1801 cgatgcgata acaccaccgt cgcgtaccag caccggcggt gtgtcgatga ttgctcgctc
     1861 cagcagatcg cgcagctcgg caaactcgcc catcttctca cgtagcgcct gtaccggtgc
     1921 actatcgaca gtttctaact gcgcacgcag ctccggcagt tgctggaaag cgtggcgcat
     1981 acgggccaga tcgcgtgggc gagcagttcg taaagccaga cgtgccagaa tacgttccag
     2041 gtcgccgacc tgacgcagta ccggctgcaa ctcggcggtg aaatcctgca atgcgccaat
     2101 agtttgctgg cgctcaagca acacgcgggt atcgcgcact ggcatatgca gccagcgttt
     2161 cagcatacga ctacccatcg gcgtgacggt gcagtcgagc acagaagcca gcgtattttc
     2221 cgcaccgccc gccaggttct gagtaatttc caggttacga cgtgtcgcgg catccataat
     2281 gatgctgtcc tgctcacgtt ccatggtgat agaacgaata tgcggcaggg tcgtgcgttg
     2341 ggtatctttc gcatactgca acagacaacc ggcagcacaa agtccgcgcg gcgcgttctc
     2401 gacgccaaaa ccgaccagat cgcgggtgcc aaattgcaga ttcaactgct ggcgcgcggt
     2461 gtcgatttca aactcccaca gcgggcgacg gcgcaggccg cgacggcctt caatcagcga
     2521 catctcggcg aaatcttctg catacagcag ttccgccgga ttagtgcgtt gcagctctgc
     2581 cgccatcgtt tcgcggtcgg ccggttcgct caggcgaaaa cgcccggagc tgatatccag
     2641 cgtcgcgtag ccgaaacctt tgctgtcctg ccagatagcc gccagcaggt tgtcctgacg
     2701 ctcctgtaac agggcttcat cgctgatggt gcctggcgta acgatacgca caactttgcg
     2761 ctcaaccgga cctttgctgg tcgccggatc gccaatttgt tcgcagatgg caacggactc
     2821 tccctgattc accagtttgg cgagatagtt ttccaccgca tggtagggaa tccccgccat
     2881 cgggatcggc tctcccgccg aagcaccgcg tttggtcagt gaaatatcca gcagttgcga
     2941 cgcgcgtttt gcgtcgtcat aaaacagttc ataaaaatca cccatccggt aaaacagcag
     3001 gatctcggga tgctgggctt tcagcctgag atactgctgc atcatgggcg tatgggcgtc
     3061 gaaattttct attgcactca tggggttatg tccggttccc tgatattaaa tggggtgtga
     3121 tggtttttat tttcgttgta atcacataag gcgcacatga taccggagtt aatcatttca
     3181 ggggaagaag cgaacggcga gttcttgatg ctggttccgg gaaagatgct gttttgcagc
     3241 ttttgcgtga tgcttatgaa gcaagtacgc aaaattgcgt cggttgaata aacctcctcc
     3301 ttcagagcca tagtcccctt tcaaattaag ttgtaatata aatacacctt aagagtatgg
     3361 aggcagcatg tccggcaagc gtatctctcg tgaaaaactg acgattaaaa aaatgatcga
     3421 tctttatcaa gcgaaatgcc cgcaggcgtc agcggagccg gagcattacg aggcgttgtt
     3481 tgtttacgcg caaaagcggc tggataaatg tgtgttcggc gaagagaaac cagcctgtaa
     3541 gcagtgtccg gtgcactgtt accagcctgc aaagcgtgaa gagatgaaac agattatgcg
     3601 ttgggccggg ccgagaatgc tgtggcgtca tccgatctta accgtgcgtc atctcatcga
     3661 tgacaagcgt ccggtaccag aactgccgga aaaatatcgt ccgaaaaaac ctcgtgaata
     3721 gtatgccgga caggcagatc tgtccggcaa tttgcagtta aatcaatgcc gatttatcaa
     3781 ttcccagccg cttcatccgt gacagcaggg tcgtgcgttt cagccccaga cgttgcgcag
     3841 cgcctttagg cccggcaacc acgccgttgg tttctttcag cacgcgcaca atcaactgat
     3901 attcatcttc gccctcctgg gcgacaaccg ttgcggcagg cggcgtttca ggttccggta
     3961 aaacaatatc tggcaatgac agctgcagca cgttaccgcg tgttagcaat accgcgcgct
     4021 caatgacgtt ttccagttcg cgtacgttac ccggccactc catgttgctc aaggtgcgca
     4081 gcgtctcggc aggaatgctg tcgatattgc gccccagacg acgggcaatt ttgaaggtaa
     4141 aggctttcgc cagcagcgga atatcttccg gacgctcgcg tagtggcggc aggtgaatcg
     4201 ggaatacgtt caggcggtaa tagagatcgc tacggaactc tcggtcggcg accatttttt
     4261 tcagatcgcg gttagtcgcg gcaattaaac gtacgtccgt ctgaatgatt ttgttgctgc
     4321 cgagacgttc aaactcctgt tcctgcaata cacgcagcaa cttcggctgt aactccagtg
     4381 gcatatcgcc cacttcgtcg aggaacagag agcttttatc cgccagttca aaacgaccaa
     4441 tgcgctgggc gctggcaccg gtaaaagccc cacgctcatg accaaacaga tcgctttcca
     4501 gcaatccggc aggcatcgcc gcgcagttca ttttgaccat gcggcgatta ttacgcccac
     4561 tgagattatg gatcgcgcgg gcaatcagct ctttacccgt gccagtttca ccgaggatca
     4621 gcacggtact gtcactttgc gccaccattt caacttgttt aagcacgctg tacatggctt
     4681 cgctgcggcc aataatctcg ccaaattcac tatcaacatt gttgagctgc tcggtcaggg
     4741 cgaggttttc atcaaccagc cgttctttca gacgatggat ttcctgatag gcgagggcgt
     4801 tatcgacagc gattgccaca cgttcggcaa tctggcgcag caaattcaga ttggtagtgg
     4861 taaacacttt ctcttcgcat tgcgccagtt tcagcacgcc cagcatggtg tcgccagaca
     4921 tcagcggtaa caggcacaag gtttgaatct ggttgcccta ggtgtcgaac aacatgcgtt
     4981 catagggggc taaatcgtcc cgctcgtgga gattgatcag cagcatctct ttacttttga
     5041 acacgcgttc ggtgagggtt ccggcttcat cgacttcgct ctgttcgtgg gcgggatgtt
     5101 gtttatcaag atagtgagtg gagtagatgt tgagtttgtt tttacggtgg ctgcgtaaga
     5161 cgatactgat atcgtcaatg tcgaaatagt aatggatttc tttggcgact tcgctgacca
     5221 gttcgtccat atccaggcgg gaaagcaccg cgttggtaat ggcaaccagg atgcggaagt
     5281 tatcgcgctc ccggcataac aactcatagt cgacattgtt gacgacgcgg ctctggattt
     5341 gttcggtgac gacagaaacg atctgcgtaa atgtttgcag acgattgaac tctttttcgc
     5401 tccagggacg atcgtcataa cgaataaatt cacagccacc aaaaatatgc ccttccgccg
     5461 ccagtggcat caggcaatag tgaccaaatt tggggtatag cccacctgcg accagctgcg
     5521 gccaggtttc acaaaattct tcgtaactgc aatgcagcgt atcagggcgc gacaaaatgc
     5581 ggcgtaccgg accgtgtgcc agaacagttt cgtcttcata tttaatgggg tgtctttttc
     5641 acgcgatgcg taataagacg cacgttgagt ctgcgcttgc cacaacacaa tagccgcgtt
     5701 gtcggcgagc gcagaacgct ttaccagttg cgaaagagcc tcacacagcg aggccagatc
     5761 gggctgctgc aatagtgtcc gagtgatgtc gaacaaccct tgctgtccga gatcactcat
     5821 cggtgtatat gacattttgt tcttccagga aagcaccgca aagcggtgca aaaggtcatt
     5881 gactaactat aggagattta gaattttatt agcatatacg cggaagaggt tcggcgtgtg
     5941 gtaaatcgag ggttcgtttc acaccataca gaccggcaag acgaacacct ttacgttcca
     6001 ccacttcacc aattagcgcc gcgtctttcc ccagtggatg ggaatgtaac gctgccagca
     6061 cttgctctgc cgcgttgcgt tcaacggcga tcaccagttt gccttcgttg gcaaagttaa
     6121 gggcgtccag tcccagcagt tcgcaaacgc cgcgcacggc aggtttaacc ggcagtgcca
     6181 cttctgaaat ttcaatacca caaccgcagg ctgccgcgaa ctcatgaact accgcgttca
     6241 caccaccacg ggtggcatca cgcagcgctt tcacgccggg aatgtcacgc agcgtctgaa
     6301 taagcggcgt cagtaccgcg cagtcgctga ccagttcgcc atccagcccc agctgctcac
     6361 gcaggttaag gatagtcgcc ccgtggtccc cgagcgtgcc gctaaccagt aatacatcgc
     6421 ctgcggttag cgtctgtgcg ccccagtgaa tattcgccgg aattgcgccc atgccagcgg
     6481 tgttgataaa cagtttatct gccgcgccgc gctgcaccac tttagtatcg ccagtaacga
     6541 tggcaatgcc tgccgcgcgc gcggtttctg ccatgctggt cactacggct ttcagtgtct
     6601 ccatcggcaa tccttcttcg aggataaagc cacaggagag atagcgcgga atagcgccac
     6661 tgaccgcaac gtcattggct gtgccgcaaa tcgccagctt gccgatatta ccgccaggaa
     6721 agaacagcgg gtcaatgacg taactgtcgg tggagaacgc cagacggtcg ccttctgcta
     6781 ccagctgcgc cagatcaaga cgtgcctggt cttcctgctc tgccagccac gggttggcaa
     6841 aggcttccat aaacaggctg ttgattaatt gctgcatcgc ctggccgccg ctgccgtggg
     6901 cgagttggat attattcacg cttcattctc ctgctgacga tactgatacc acgcggcgca
     6961 cgctccttcg gaggagacca tcagtgcacc aaatgcggtt tgaggattac aggtgttacc
     7021 aaacagcggg cattgatgcg gcttacattt gcccgttaat acctcaccac aacgcgcgcg
     7081 cgggtcatcg cagacctgct gcggtgctgg gcggaaatgt gcttcggcat cgaatcgttg
     7141 ataatccggc gtcaggtgca cgccagaaga ttcaatcacg cctaagccgc gccattcgct
     7201 gtcgccgttg acacagaaca catcagcaat tgcctgttgc gccagcaggt taccggcatc
     7261 cggtaccacc cgacgatact gattctctac cttgctgtgg gccgctattt tctgctccac
     7321 cagcatgacc acgccttgca gtagatcaag gggttcgaaa ccagcgacca ccagcggacg
     7381 atgaaaatcg ctggcgataa aattataggc atcagtgccg ataaccatac taacgtggcc
     7441 cggcgcgagg aacgcgtcga taccgttatc cggctgttcc agcaaactgc gcagcgtcgg
     7501 gataagcgta atatgctggc agaagaagta aaaattctgc acatcgcggg ctttcgcctg
     7561 ttgcagggtg atggcggtgg tcggcatggt ggtttcaaaa cctaagccga agaatacgac
     7621 tttacgggtt ggattctcct gcgccagttt caacgcatcc atcggcgagt aaacgatgcg
     7681 cacatcggca ccgcgtgctt ttgcctgcaa cagcgatccc tgtttccccg gcacgcgcat
     7741 ggcgtcgcca aaggtacaga agatgacttc cggatggctg gcaatctcca cgcaggtgtc
     7801 gattctaccc atcggcagta cgcacaccgg gcaacccgga ccgtggataa actcaacgtt
     7861 ttcaggcagt aactggtcga ggccgaattt aaaaatggcg tgggtatgac caccgcacac
     7921 ttccataatc cgcagagggc gttcggcggt gtaagagaga tgtgaagcac gttcgcgcag
     7981 atgctcaatt aactgcatca cctgttccgg cgcgcgatat tcatcaacaa aacgcattat
     8041 ttttcctcgc catacaacag cgcgccgaca tccggctcaa cgtcaaacat gttttgtaag
     8101 gcgtcgagag tgtcgcgtgc ttcggcttca ttaattacgc tcatggcaaa gccaacgtgt
     8161 accagtaccc actggcccac gcgcggctga ccgttttcat cgcagctgcc gactaacgtt
     8221 aaatcgacat cgcgctgaat gccgcagacg tcgacttttg cctggttgcc gtcaatggtg
     8281 cggatctggc cgggaacgcc tatgcacatc gctgtgtctc cagccagttc agccactggt
     8341 ccatcccttc gccgctggtg gcggaaataa ggatgatttc aatttctgga ttgacttcgc
     8401 gggcgcaggc gatgcacttc tcaacgtcaa agttgagata cggcaacagg tcaactttgt
     8461 tgagcagcat cagcgaggcg gcggcaaaca tatgcggata tttcagcggt ttgtcttcac
     8521 cttcggtaac ggaaagcacc gccactttgt gtttttcacc gagatcgaag ctggccgggc
     8581 atacgaggtt gccaacgttt tcgataaaca gaataccgtt atcgtccagt ggcagacgcg
     8641 gtgcggcgtc ggcaatcatc tgtgcgtcaa gatggcagcc tttaccggtg ttcacctgaa
     8701 tcgctggtgt gccggtagcg cgaatgcgtg cggcatcgtt cacggtttgc tggtcacctt
     8761 caataaccgc gcacggaacg ctgtctttca ggcgcattag ggtttccgtc agcagggtgg
     8821 ttttaccgga accagggctg gaaaccaggt tgagcaccag ttgcttgcgg gcagcaaagc
     8881 gcgcgcggtt gcgttcagcc agacggttat ttttgtccag cacgtcaatt tcgacttcca
     8941 gcatccgacg ctggctcatg cccggcgcgt gagtgcccgc ttcgccatga ccgtaatgca
     9001 ggtcgccttc ttcagtctgg ctgggggtaa attcaggcgc tttaatgccg gtgattttca
     9061 tcttcgggcg tgccgccggg gcaaatggcg cgctacgaaa cgcggaatga gggttatgtt
     9121 catcaccctc gatatacagg ttgccttcac cgcaaccgca tgttgtacac atcgctcact
     9181 cctggtctat ttctatccgc cgaatctgta aaccgtcgtc tgccacaatc tgtagcatgt
     9241 caccatgaca ctgtggacag cggcggacgc gctgggtcag tagcgtcaca tactgttggc
     9301 atgtttcaca ccagcattcg gcctcttgtt cttcgaggtg cagtttacaa ccttccgcca
     9361 cgctgccgcg gcaaaccaga tcaaaacaaa aggcaagaga gctggtttcg acacaagaaa
     9421 atgcgccaat tttgagccag accccagtta cgcgttttgc gccgtgtttt gcggcctgct
     9481 gttcgatcaa ttccagtgcc cgttggcaga gggttatttc gtgcatatcg cctcccatta
     9541 actattgcca gctacaagca ataattgtgc cagtgttgat tatccctgcg gtgaataatg
     9601 tcgatgatgt cgaaatgaca cgtcgacacg gcgacgaaat tcatctttag cttaaaaatc
     9661 tatttaataa caataaatta aaagttggca tagaaaatgc ttaaagctgg catctctgtt
     9721 aaacgggtaa cctgacaatg actatttggg aaataagcga gaaagccgat tacatcgcac
     9781 agcggcattg tcgcctacag gaccagtggc acatctactg caattcgctg gttcagggga
     9841 tcacgttatc gaaagcgcgc ctgcatcacg ccatgagctg cgcgccggac aaagaactct
     9901 gtttcgtcct ttttgaacat tttcgtattt acgtcaccct ggcggatggc tttaacagcc
     9961 acaccatcga gtattacgtc gaaacaaaag atggcgaaga caaacagcgg attgcgcagg
    10021 cgcaactgag cattgacggc atgattgatg gcaaggtcaa catccgcgat cgcgaacagg
    10081 ttctggaaca ctatctcgaa aaaatcgctg gcgtttacga cagcttatac accgctattg
    10141 aaaacaatgt gccggtgaat ttaagccaac tggtaaaggg acaaagcccg gcagcatgag
    10201 ctgaggcttt gcccgttttg caggcgttac gcctgtttgg ggatgggcgt gtcgatgagt
    10261 gtcgaaaatg acatttcatc ggcatgtttt cgtcaaaaat gacaatcacc tgaggaatgc
    10321 ctggtgaatc gttttgtaat tgttgactcc acgctctgta tcggctgcca cacttgtgag
    10381 gccgcctgtt cagagacgca tcgccagcac ggcctgcaat caatgccgcg cctgagagtg
    10441 atgctgaatg aaaaagaatc tgcgccgcag ctctgtcacc actgtgaaga tgcaccctgt
    10501 gcggtggttt gcccggttaa cgccatcacc cgcattgatg gggccgtgca gttgaatgaa
    10561 agtctgtgcg taagctgcaa actgtgcggc atcgcctgcc cgtttggcgc aattgaattt
    10621 tccggcagcc gtccgctgga tattccggca aacgccaata ccccgaaagc gccaccggca
    10681 ccgcctgctc cggcgcgtat cagtacgctg cttgactggg tgccgggtat tcgcgcgatc
    10741 gccgtcaaat gtgacctttg cagttttgat gaacaaggtc cggcctgcgt gcggatgtgc
    10801 ccgactaaag ccctgcatct ggtggataac accgatatcg cccgcgtcag caaacgtaag
    10861 cgtgagctga cctttaacac ggactttggc gatctcacct tgtttcagca ggctcaaagt
    10921 ggagaggcta aatgagcgca atttccctga tcaatagcgg cgtggcatgg tttgtcgccg
    10981 ccgctgttct ggcatttctc ttttcttttc aaaaagcgtt aagtggctgg atagctggaa
    11041 ttggcggcgc ggttggtagt ctgtatacgg cagccgcggg cttcactgta ctgactggcg
    11101 cggttggcgt gagcggtgcg ctgtcgctgg taagctacga tgtgcaaatc tctccgctta
    11161 acgcgatttg gctgattacg ctcggtctgt gcggtctgtt tgtcagcctc tacaacattg
    11221 actggcatcg ccacgcgcag gtgaagtgca acggcttgca gatcaatatg ttgatggctg
    11281 ccgccgtctg cgccgtcatc gccagcaacc tcggcatgtt cgtggtaatg gccgaagtca
    11341 tggccttgtg tgcggtgttc ctcaccagca acagcaaaga gggcaaactg tggtttgcgc
    11401 tggggcgtct tggcactctg ctgctggtga ttgcttgctg gctgctgtgg cagcgttatg
    11461 gcacgctgga tctgtgccta ctggatatgc gtatgcaaca gctgccgctc ggttccgata
    11521 tctggctgct cggtgtgatt ggctttggcc tgctggccgg gattattccg ctgcacggct
    11581 gggtgccgca ggcacatgct aacgcctctg cgccagctgc cgcgttgttt tctacggtag
    11641 tcatgaaaat tggcctgctg ggcatgttaa ccctgtcact gctgggcggt aatgcaccgc
    11701 tgtggtgggg gatcgcgctg ctggtgctcg gcatgatcac cgcgttcgtc ggtggtctgt
    11761 atgcgttgat ggaacacaac atccagcgcc tgctggctta ccacaccctg gaaaatatcg
    11821 gcattatcct gctggggctg ggcgctggcg taacgggtat cgcgctcgaa caaccggcgc
    11881 tgattgctct tggcctggtc ggtggtctgt accatctgct taaccatagc ctgttcaaaa
    11941 gcgtactgtt cctcggggcg gggagcgtct ggttccgtac cggtcatcgc gatatcgaaa
    12001 aactcggtgg tattggcaag aaaatgccgg ttatctccat cgccatgtta gtcgggttga
    12061 tggcaatggc tgcgctgccg ccactgaacg ggtttgccgg ggaatgggtt atctatcaat
    12121 ccttcttcaa actgagcaat agcggcgcgt ttgttgcccg tctgctgggg ccgctgctcg
    12181 ctgtgggtct ggcaattacc ggtgcgctgg cggtgatgtg tatggcgaaa gtctatggcg
    12241 tcacgttcct cggcgcgccg cgtaccaaag aagccgaaaa cgccacctgt gcgccgctcc
    12301 tgatgagcgt aagcgtagtg gcactggcga tttgctgcgt aattggcggt gttgctgcgc
    12361 cgtggctgct gccgatgctc tctgctgctg tacctctgcc tctggagcct gctaacacca
    12421 ccgtttctca accaatgatc acattgctgc tgattgcctg cccgctgctg ccattcatca
    12481 ttatggcgat ttgcaaaggc gaccgcctgc cgtcacgttc ccgcggtgcg gcctgggtgt
    12541 gcggctacga ccatgaaaaa tcaatggtga ttaccgctca cggttttgcc atgccggtta
    12601 aacaggcgtt tgcgccagtg ctgaaactgc gcaaatggct gaatccggtg tcgctggtgc
    12661 cgggctggca gtgcgaaggt agtgcgttgc tgttccgccg gatggcgctg gttgaactgg
    12721 cggtactggt ggtgattatt gtttcacgag gagcctgaga atgagtgttt tatatccgtt
    12781 aattcaggcg ctggtgttat ttgccgttgc gccgctgctc tccggtatta cccgcgtggc
    12841 gcgcgcccgt ctgcataacc gtcgtgggcc gggtgtgttg caggagtatc gtgacatttt
    12901 caaactgctg ggccgccaga gcgttggccc ggatgcctcc ggctgggtgt tccgcctgac
    12961 gccgtatgtg atggtgggcg tcatgctgac tatcgctact gcgctgccgg tggtgaccgt
    13021 cggttctccg ctgccgcaac tgggtgattt aatcacctta ctgtatctct ttgccatcgc
    13081 gcgtttcttc tttgccattt ctggtctgga taccggtagc ccgtttaccg ctatcggcgc
    13141 gagccgtgaa gcgatgcttg gcgtgctggt cgaaccgatg ctgctgcttg gtctgtgggt
    13201 tgccgcacag gttgccggtt ccaccaacat cagcaacatc accgacaccg tttatcactg
    13261 gccgctgagc cagagcatcc cgctggtact ggcgctttgt gcctgtgcgt tcgccacctt
    13321 tatcgaaatg ggcaaactgc cgttcgacct ggcggaagcc gagcaggagc tgcaggaagg
    13381 cccgctctct gaatacagcg gcagcggctt tggcgtcatg aaatggggta tcagcctgaa
    13441 acagctggtg gtgctgcaga tgttcgtcgg cgtatttatc ccgtggggac agatggaaac
    13501 cttcaccgcc ggtgggctgc tgctggcgct ggtgattgcc atcgtcaaac tggtcgtcgg
    13561 cgtcctggtt atcgcgctgt tcgaaaacag catggcccgt ctgcgtcttg atattactcc
    13621 gcgcattacc tggactgggt ttggctttgc atttttagcg ttcgtctcct tgctggcggc
    13681 gtgattaaag agagtttgag catgtctgaa gaaaaattag gtcaacatta tctcgccgcg
    13741 ctgaatgagg catttccggg cgtcgtgctg gaccacgcct ggcagaccaa agatcagctg
    13801 actgtcaccg taaaggtgaa ctatctgccg gaagtggtgg agtttctcta ctacaagcag
    13861 gggggctggc tgtcggtgct ttttggtaac gacgaacgca aactgaatgg tcattacgcc
    13921 gtttattacg tgctgtcgat ggagaagggg actaagtgct gggtaacggt tcgcgtcgaa
    13981 gttgatgcca acaaaccgga gtatccgtcc gtgacgccgc gcgttccggc ggcggtgtgg
    14041 ggcgagcgcg aagtgcgcga tatgtacggt ttgattccgg ttggtctgcc ggatgaacgc
    14101 cgtctggtgc tgccggatga actttatccg ctgcgtaaag acagcatgga ttatcgtcag
    14161 cgtccggcac cgaccaccga tgctgaaacc tacgagttca tcaacgaact gggcgacaag
    14221 aaaaacaacg tcgtgccgat tggtccgctg cacgtcactt ctgacgaacc gggccacttc
    14281 cgtctgttcg tcgatggcga aaacattatc gacgccgact accgtctgtt ctacgttcat
    14341 cgcggcatgg aaaaactggc ggaaacccgt atgggttaca acgaagtgac cttcctctct
    14401 gaccgtgtgt gcgggatctg cggctttgcc cacagcaccg cctacaccac gtcggtagaa
    14461 aacgcgatgg gtattcaggt gccagaacgt gcgcagatga tccgcgccat tctgctggag
    14521 gtggaacgcc tgcactcgca tctgctcaac ctcggtctcg cctgccactt taccggcttt
    14581 gactccggct ttatgcagtt cttccgcgtg cgtgaaacct ccatgaaaat ggcagagatc
    14641 ctcaccggtg cgcgtaaaac ctacggcctg aacctgatcg gcgggattcg tcgcgatctg
    14701 ttgaaagatg acatgatcca gacccgccag ctggcgcaac agatgcgtcg tgaagtgcag
    14761 gagctggtgg atgtgctgct gagtacgccg aacatggaac agcgcaccgt cggcattggt
    14821 cgtctggacc cggaaatggc ccgcgatttc agtaacgttg gcccgatggt tcgcgccagc
    14881 ggtcacgccc gcgatactcg cgccgatcac ccgtttgtcg gttatggcct gctgccaatg
    14941 gaagtccaca gcgagcaggg ctgcgacgtt atttcccgtc tgaaagtgcg tatcaacgaa
    15001 gtttataccg cgctgaacat gatcgactac ggtctggata acctgccggg cggtccgctg
    15061 atggtggaag gctttaccta cattccgcac cgtttcgcgc tgggctttgc cgaagcgccg
    15121 cgcggcgacg atatccactg gagcatgacc ggcgacaacc agaagctgta ccgctggcgc
    15181 tgccgtgccg cgacctacgc gaactggccg accctgcgct acatgctgcg cggtaacacc
    15241 gtttccgatg cgccgctgat tatcggtagc ttggaccctt gctactcctg taccgaccgc
    15301 atgaccgtgg tcgatgtgcg taagaaaaag agcaaagtgg tgccgtacaa agaactcgag
    15361 cgttacagca ttgagcgtaa aaactcgccg ctgaaataag gaatcgccat gtttaccttt
    15421 atcaaaaaag tcatcaaaac cggcacggcg acctcgtctt atccgctgga gccgattgcg
    15481 gttgataaaa acttccgtgg taagccggag cagaacccgc agcagtgcat cggctgtgcg
    15541 gcctgcgtca atgcctgccc gtcaaacgcc ttaacggttg aaactgacct cgccaccggt
    15601 gagctggcgt gggagtttaa tctcgggcgc tgcatcttct gtggacgctg cgaagaagtt
    15661 tgcccgacgg cggcgatcaa actgtcgcaa gagtacgaac tggcagtgtg gaagaaagaa
    15721 gacttcctgc aacagtcccg cttcgcgctg tgcaactgcc gcgtctgcaa tcgtcctttc
    15781 gccgtccaga aagagatcga ctacgccatt gcgctgctta agcacaacgg cgacagccgc
    15841 gcggaaaacc accgcgaaag ctttgagact tgcccggaat gtaagcgcca gaaatgcctg
    15901 gtgccgtccg accgtattga actgactcgc catatgaaag aggccatctg atgagcaatt
    15961 tattaggccc ccgtgacgcc aacggcattc cggtcccgat gacggtggat gaatccatcg
    16021 ccagcatgaa ggcgtcgtta ctgaaaaaaa tcaaacgttc tgcctacgtt tatcgcgtgg
    16081 actgcggcgg ctgcaacggc tgcgaaatcg aaattttcgc cacgctttcg ccgctgtttg
    16141 atgcagaacg cttcggcatt aaagtcgttc cttcaccgcg tcatgcggat attttactgt
    16201 ttaccggcgc ggtcacccgt gcaatgcgat cccctgcgct gcgtgcgtgg cagtccgcgc
    16261 cggacccgaa aatttgtatc tcctacggtg cctgcggtaa cagcggtggt attttccacg
    16321 atctctactg cgtgtggggc ggtacggata aaatcgtccc ggtagatgtc tacattcctg
    16381 gttgcccgcc aacgcctgcc gccacgctgt acggctttgc aatggcgctc ggcctgctgg
    16441 agcagaaaat tcacgcccgt ggcccgggtg agcaggatga acaacaggcg gaaatcctgc
    16501 atggcgatat ggtgcagccg ctgcgtgtga aagtggatcg cgaagcacgc cgcctggcgg
    16561 gttatcgtta cggtcgtcag attgccgatg acttcctgac acagttaggg cagggcgaag
    16621 aacaggttgc acgctggctg gaagcggaaa acgatccgcg tctgaacgag attgtcagcc
    16681 atctgaatca tgttgttgaa gaggcgcgta tccgatgagt gaaaaggtgg tgttcagtca
    16741 actgagccgt aaatttattg atgagaacga tgccacgccc gccgaggcgc agcaggtggt
    16801 ctattacagc ctggcgattg gtcaccacct tggggttatc gattgcctgg aagcggcgct
    16861 cacctgcccg tgggatgaat atctggcatg gattgccact ctggaggcgg gcagtgaagc
    16921 ccgccgcaaa atggaaggcg tgccgaaata tggtgagatc gtcatcgaca ttaaccatgt
    16981 gccgatgctg gccaacgcat tcgataaagc ccgggcagcg caaacttcgc agcagaagga
    17041 atggagtaca atgctgttaa gtatgctgca tgatattcat caggaaaacg ccatctattt
    17101 gatggtgagg agactgcgtg actgacgttt tactctgtgt tggcaatagc atgatgggcg
    17161 atgatggcgc tggcccgctg ctggcggaaa agtgcgccgc cgcgccgaaa ggtaactggg
    17221 tggtgattga tggcggtagt gcaccggaaa acgacatcgt cgccatccgt gaactgcgcc
    17281 ctacgcggtt actgattgtt gacgctactg atatggggct aaaccccggc gaaatccgca
    17341 tcatcgaccc ggatgatatc gccgagatgt ttatgatgac tacccataac atgccgctga
    17401 actatcttat cgaccagttg aaagaagaca ttggcgaagt gattttcctc ggcatccagc
    17461 cggatatcgt cggcttttac tacccgatga ctcagccgat taaagatgcg gtagaaaccg
    17521 tttatcaacg actggaaggc tgggaaggga atggcggctt cgcgcagtta gcggtggaag
    17581 aagaataatt ctccatcagg gaattcagcg aaatgaatat cagaacaggg atgtgcgctt
    17641 ttttattgag cttagtgctg cctgcacagg ccaccagctt taccgaatat ctgccaatga
    17701 gtgacagcga atacgcgcaa aagcgggcgc tgaaacctct cctgaccatg ccttatgatg
    17761 ccgagcaaac ctggcgtttt agaaaagtgg gcgttgctgg cgtgacgctt gaaaagatgc
    17821 caaatgatga tagtgaatgg caattgaacg gtaaagatcg cgccggtaaa tcctggtctg
    17881 tgcctgtggg tatgctggca aacatagcgg gcaaagggca actctatcgc gccgatcttg
    17941 atcgtaacgg cattcaggat ctggttatct ggttaccaag taccggtaat ggtctggctc
    18001 cttatgcaca tttaattctt atgacgttca cgcgcgaagg ccgtccctgt gtctttgaac
    18061 ccaggggatt ctacaccgca agcaaaactg gcgtagatga tttgctggat ttgcaaggca
    18121 atggtcacac gcaattgctg gatatgcagt ttgattccgg ttactggata accagtctgt
    18181 atcaggtaaa agacgccaaa tggcagcgtg ttcacggctg gtttggcaag ttaagctatc
    18241 ccgcgctcac gcgttttact tataccccca accgtaaact cgtcctcaaa cccatcgccg
    18301 ggcgcgaccc gcaaacggaa gatctggcgc agacgcagcg ttgcttgata aagggggatg
    18361 ttcttgatgg ggtgaaccag aactgacttt ttgcagagtg gctatgagga ttttgggggt
    18421 aattatatcg cgctcaagtg tttacatatt gtgaacttgg atttgatgag ctatagtttc
    18481 cgaacacata aggatagaac tcgcagatgt ggctgtttac ttactataag gaagttaaaa
    18541 cttaaaaatg cacatcattt ctaaagcgcc atttgaagag tctgcgcgta aatatcctaa
    18601 cgacgctctg gctcttcagg cattgtatcg ggttattaag gaaacggatt tttctacgcc
    18661 agaagagatg cgaacggcgt ttcccaacct cgacaatttt aaatatcgca acaaatggta
    18721 cgttctggat gtaggtggga acaacctaag ggtgattgcc tacataaact ttgttaataa
    18781 gcggttcttt gtgaagcaca taactaatca cgccgagtat gacaaactga cacgttacta
    18841 cagagagaac aaagaatgac tgctaatgct gcacgcgctg ttaaagcaac aagagaactg
    18901 gtcaatgcag tcccctttct gggaggtagt gactcagaag atgattatcg tgaagcactg
    18961 gaactggtcg aatatttgat tgaagaagat gacaccaatc cacttatcga ctttctggcg
    19021 agccgcattg cagaatatga aaacaacaac caagagtttg cagagttcga caaagccgtt
    19081 gcggcaatgc ctgttggcgt ggcgttactt cgcacgctga ttgatcaaca taatctgacc
    19141 tatgcggatt tgaagaacga aatcggctct aaatccctgg tcagtcagat tttgtctgga
    19201 caacgatccc tgacgatatc ccacattaag gcactttcag cccgatttgg cgttaagccg
    19261 gaatggtttc tttgattttg actgatgcca aaccccagat tgcccgatgc gctgcgctta
    19321 tcgggccttc ctggttcgtg cgacatgtag gccggataag gcgttcacgc cgcatccggc
    19381 actgttacct actctaaatc ttccccatta ctggcaatca cttttttata ccaccagaac
    19441 gatttcttac gtgtgcgcgt cagcgtgccg ttgcctgcgt cgtcacggtc tacgtagaca
    19501 aaaccgtagc gtttgctcat ttcacccgta gaggcggaga ctaaatcaat acagccccag
    19561 gtggtgtagc ccatcagcgg aatgccatct gcaatcgctt cgcccattgc gcggatatgt
    19621 tcgcgtaaat agctgatgcg atagtcatcg ttaatctcgc cattggcagc aagttcatct
    19681 tttgcgccga ggccgttttc caccagaaac agcggcttct gataacggtc gtacatcatg
    19741 ttcatggtga tacgcagacc gagtggatca ataccccagc cccagtcgct cacttgcaga
    19801 tacgggttac gcagcgattt cactacgttc gccgcactgc tattgttggc gttcatctcc
    19861 gccgaggcgc agcgcgaggc gtaatagctg aaagagacaa aatcgacggt gtttttcagg
    19921 atttcatcat cgcccggtgc tttgtcgatg gttacccctt tttcgcggaa tacgcgggca
    19981 gagtaagccg gatacgtgcc ccgcgcctgc acatcgataa aaaacaggtt ttcccggtct
    20041 ttctccagcg ccgcccagac atcttccggc ttgcaactgt aagggtagaa gttaccgccc
    20101 gccagcatac agcccacctg attttgcggg ttaacctcat gggcgatttt ggtggctagc
    20161 gcactggcaa ccagctggtg atgcgcggcc tgatatttca cctgatcctg attttcacct
    20221 tcttcaaaca ccagacccgc gccggagaac gggctatgca acataatgtt gatttcattg
    20281 aaggtaagcc agtatttcac cagaccatca aatgcttcaa agcaggtccg ggcgtagcgg
    20341 ctgaaaaact ccaccagctt gcggttacgc caggagccat attcggtgac cagatgcatc
    20401 ggcacatcga agtggcacaa cgtgaccagc ggttcgatac cgtacttttt acactcttca
    20461 aagacagaac gataaaaagc aatgccctgc tgattgggcg tgatttcatc gccctgcgga
    20521 aagagacggc tccaggcaat tgaggtacgg aaaaccttga atcccatctc tgccatcagg
    20581 gcgatatctt ctttataacg atgataaaaa tccgtcgcct catggctggg ataaaactcg
    20641 tcatcgcgca actgaaaacg tttttccagc cccagtttca ccgccattcg atgctcgccg
    20701 tgtgggatca tatcgacagt ggtcagccct ttgccacctt cacggaacgc accttcagac
    20761 tggttggcgg caagcgcgcc gccccataaa aaaccttctg gaaatactga cattttcatc
    20821 ctcaattaag acttacttct ttggcgacgg tcggttgtac gctctgatgc tttcgcgcct
    20881 cggcagccgc ttcctcaaca ggaatatcct caaagccgag caacagtgtg aggataaacg
    20941 acagcaccac cgccagcgcc atgaccgcga acacccagac gatgctcatt ggattcgccg
    21001 gatcgaagaa ttgcacgctg gtaaataacc ctggcgctgc cattgagtgg ctggcaagcc
    21061 ccgccatacc ggcaaccgcg ccgcaaataa aaccgctgat aagactggcg ataagcggac
    21121 gtttcaggcg gatcgccacg ccgtataacg ccggttcgga aatccccgcc ataatggctg
    21181 atgccgccgc agccagcgcc gtctggcgca gttccgggtt tttcgttttc cacgccaccg
    21241 ccagtgatga accgcccagc gacaggttag cgccgatctc tgacggcatg accatccctt
    21301 ctttgccggt ttcggcaatg gtctgaatga tggttggcgt aaagacgcgg tgcatcccgg
    21361 tcattaccag cagaggccac agcgcgccca taatggcgac tgaaagccag cccagataac
    21421 catgaatggt gtaaaccagc gccgaaatgg cgctaccgat ccagatacca atcgggccaa
    21481 tcagcaggat tgccagcggt gcggcaatca acacaatcag cattggcttg aggaagtttt
    21541 tggtcaccgc cggtgtaatg cgatccaccc agcgttcgat atatgacagg caccaggtca
    21601 tcaccagcgc cgggatcact gtgtaggtgt atttcaccgc ggtgaccgga atcagggcaa
    21661 attcgacatg ttcaccctgg gccgctttcg ccatcagttc aataaaactc ggatgtacca
    21721 gcacacccgc gatggcaatc gccagcgaca tattggtttt aaatttgatg gcggcagatg
    21781 ccgcgaccat cagcggcagg aagaagaaag caccgtcacc aatcacattc agaatggtta
    21841 aggtcggcga tccttttgtc agcacgccgc tcatctcgag gatcatcgcc agcagtttga
    21901 ccatcgatcc gccgataatc gccgggatca gcggtgacat ggtgccgatc agcgcatcga
    21961 ggatccccgc accaatgcga cgcagcgtga gtttgggttt acccacgggc tgtgcgggct
    22021 gcatatctcc cggcagcagg ctgacgactt cctgaaaggc ttgtgaaacg gtattgccga
    22081 taatcacctg acactggttg tcactacgta ccacgccgag cacgccgggg atggttttta
    22141 acgtcgggct gtcgataagc tggtcatctt tgataacaac gcgcaaacgc gtcatacagt
    22201 gcgtgaccgc cgagatgtta tcaacgccgc ccagtgccgc tatcaccgag cgtgccagcg
    22261 ccgcataatt tttggccatc ggattttatc ctgttatcag tagggtagat gtagttgtta
    22321 tcagtcgacg taacggaaaa atgccgatgc tgaaacggaa ttaggaaacc ggtttcacaa
    22381 aatcatgaat agttgtcact aaataagcaa gagagttaat tgttcatttt ttatatttgt
    22441 gaaaccggtc acctgaatat ttttcccgcc gattgcgcaa ggacaatcgg caaacggtgc
    22501 agtacactgc gttgattgcg tgtaaaggga tgaaaatgat gacgacgatg ctggaagtgg
    22561 cgaagcgcgc cggggtttca aaagcgaccg tttcccgcgt gctttcaggt aatggctacg
    22621 tcagccagga gactaaagat cgcgtgtttc aggcggtaga agagagcggt taccgcccaa
    22681 acttgctggc gcgcaatctg tcggcgaaga gtactcagac gctggggttg gtagtgacca
    22741 acacgcttta ccacggcatt tattttagtg aattactctt tcatgccgcg cgaatggcgg
    22801 aagagaaagg gcggcagttg ctattggcag atggtaaaca cagcgccgaa gaagagcgcc
    22861 aggcgattca gtatctgctg gatttgcgct gcgacgccat tatgatttac ccgcgttttt
    22921 taagcgtgga tgagatcgat gacatcattg acgcgcacag tcagccgata atggtgctta
    22981 atcgccgcct gcgcaaaaac agcagccata gcgtctggtg cgatcataaa cagaccagct
    23041 ttaatgccgt ggcagagttg ataaacgccg gacatcagga gattgctttc cttaccggct
    23101 cgatggattc ccccaccagc attgaacgtc ttgctgggta taaagacgcg ctggcgcagc
    23161 atggtattgc gctcaatgaa aaacttatca ctaacggtaa atggacgccc gccagcgggg
    23221 ccgaaggggt agaaacgttg ctcgaacgtg gggctaaatt tagcgcgtta gttgccagta
    23281 acgacgatat ggcgataggt gcgatgaaag cgttgcacga gcgcggcgta gcggtgccag
    23341 agcaggtgtc agttatcgga ttcgatgata tcgcaattgc cccctatatc gttccggcgc
    23401 tctccagcgt aaaaattccg gtgactgaga tgattcagga aattattgga cggctgattt
    23461 ttatgctcga tggcggggat ttctcaccgc cgaaaacctt cagcggaaaa ctgatccgcc
    23521 gcgactcact cattgctctt tcgcgataaa cattgtcatc acatgtcatc gtcactagtg
    23581 tcgatgacat cttttttcct gcattacaga aattaatttc cttaaataac agtgaattaa
    23641 aactggcatg atttgtgaat gtatcggcgc attaactgtc attgctggag aatttgatga
    23701 accgtttcat cattgctgac gcgagtaaat gtattggttg ctgtacctgt gaagtggcgt
    23761 gcgtggtttc tcatcaggaa aatcaggact gtgcatcgct gaccccggaa acatttttac
    23821 cgcgtatcca tgtcattaaa ggtgtgaaca tttccacggc gacagtttgc cgtcagtgtg
    23881 aagatgcacc gtgcgctaac gtctgcccga atggtgctat cagccgtgat aaagggtttg
    23941 ttcatgtgat gcaggaacgt tgcattggtt gcaaaacctg cgttgtggct tgcccgtatg
    24001 gcgcgatgga agtggtggta cgtccggtga ttcgcaacag cggcgcgggg ctgaatgtac
    24061 gggctgacaa agccgaagcc aataaatgtg acctgtgcaa ccatcgtgaa gacggcccgg
    24121 cgtgtatggc ggcttgcccg actcatgcgc tgatttgtgt cgatcgtaat aaacttgaac
    24181 aactgagtgc agaaaaacgc cgccgtacgg cgctgatgtt ctaaaaatct tacagaactg
    24241 ttctggcatc aaaggaattc ccggaaatat tcgtagaata cggagctggc ggtatcgccg
    24301 acctgagact gccggatacg acgcagtcgt gtccggcagg acgggttacg caattcatga
    24361 tggcgggaat aactcaatgg caaaaaacac atcttgcggt gtccaactgc gtattcgtgg
    24421 caaagtgcag ggcgtcggtt ttcgtccgtt tgtctggcag ctggcacagc aattaaatct
    24481 tcacggcgat gtctgtaatg acggcgatgg cgtagaagtc cggctgcggg aagacccgga
    24541 aacgttactt gttcaattgc atcagcactg cccgccgctg gcgcgtattg atagcgtcga
    24601 gcgtgagccg tacatctggt cacaactgcc cactgagttc accatccgcc agagcgcggg
    24661 aggtgccatg aatacgcaaa ttgtcccgga tgccgctact tgccctgctt gccttgccga
    24721 aatgaatacc ccaggcgaac ggcgttatcg ttatccgttt atcaactgta cccactgcgg
    24781 cccgcgtttc accattattc gcgccatgcc ttacgaccgc ccgtttaccg tgatggcggc
    24841 gtttccgttg tgtccggctt gtgacaaaga gtaccgtgac ccgctcgatc gtcgcttcca
    24901 cgcccagccg gtggcctgcc cggagtgtgg cccacatctt gaatgggtaa gtcatggtga
    24961 acatgcagaa caagaggcgg cattacaggc ggctatcgca cagttaaaaa tgggcaacat
    25021 tgtcgccatc aaagggattg gcggatttca tcttgcctgc gatgcacgta acagtaacgc
    25081 ggtggcgaca cttcgggcgc gcaaacatcg cccggcgaaa ccgctggcgg tcatgttgcc
    25141 agtggctgac ggtttaccag acgctgcgcg ccagttgctt accacgcccg ccgcgccgat
    25201 tgtgctggtg gataaaaaat acgttcctga gctttgtgat gatatcgccc ctgaccttaa
    25261 cgaagtcggg gtaatgttgc ctgcgaaccc gctccagcat ttgctgttac aggaactgca
    25321 atgcccgctg gtgatgacct ccggcaacct gagcggtaaa ccaccagcta tcagcaacga
    25381 acaggcgctg gcggatttgc agggcattgc cgacggattc ttgatacata accgcgacat
    25441 cgtgcagcgg atggatgatt cggtggtgcg cgaaagcggc gaaatgctgc gccgttcgcg
    25501 ggggtatgtg ccggatgcgc tggctttgcc tccgggcttt aaaaatgttc cgcctgtgct
    25561 gtgtctcggc gcggatctga aaaatacctt ctgcctggtg cgcggtgaac aagcggtgtt
    25621 gagtcagcat ctgggcgatt taagtgacga tggcatccag atgcagtggc gcgaagcgtt
    25681 acgcctgatg caaaacatct acgattttac cccgcaatac gttgtgcatg acgcgcatcc
    25741 gggctatgtc tccagccagt gggcgcgtga aatgaatctg ccgacgcaaa cggtactgca
    25801 tcatcatgcc catgcagcgg cgtgtctggc agagcatcag tggccgttgg atggcggtga
    25861 tgtcattgct ttgacgctcg acggtatcgg tatgggggag aacggcgctt tgtggggcgg
    25921 cgagtgcctg cgggtgaact atcgcgaatg tgagcacctg ggcggcttgc ctgcagtggc
    25981 gcttccgggt ggcgatttgg cagcgaagca gccgtggcga aacctgctgg cgcagtgcct
    26041 gcgctttgtg ccggagtggc agaattaccc tgaaacagca agtgtgcaac agcaaaactg
    26101 gagcgtgctg gcgcgggcca ttgagcgtgg aattaacgcg ccgctggcgt catcgtgtgg
    26161 gcggttgttc gatgcagtgg cggcggcact gggctgtgcg ccagccacgt taagttatga
    26221 aggtgaagcg gcttgtgctc tggaggcgct cgcagcctca tgccacggag tgacgcatcc
    26281 ggtgacgatg ccgctggtgg acaatcaact ggatctcgcc gctttctggc agcagtggct
    26341 gaactggcag gcaccggtta atcaacgcgc gtgggcgttt catgatgcgc tggcgcaggg
    26401 ttttgccgcg ttgatgcgtg agcaggccac gatgcgtggt atcactacgc tggtatttag
    26461 cggcggggtt attcataacc gtttgctgcg tgcacgtctg gcgcattatc tcgctgattt
    26521 cacattgctc tttccacaga gtttaccggc gggtgatggc ggtttgtctc tggggcaggg
    26581 ggttattgct gcggcgcgtt ggttagcggg tgaagtccag aacggataaa acgtgttgca
    26641 tttggacacc attgccggat gcggcgtaaa cgccttatct ggcctacaca tgcgtccccg
    26701 taggtcggat aagacgcgcc agcgtcgcat ccgacatttt aggcacagta gcccacctac
    26761 atcggcaatg ttttcaacaa tccaaatgcc tctttcatcc gatcctcact gaccacaaag
    26821 gcgcgaagct ggtcagcatc gtcaacaccg cgagccacca ttccctggcg ttcggtatta
    26881 atttgccagc gtaaatcctg acgctgggtt tcgcctgcca gatgcagcgg taattccggc
    26941 gttttgattt tcaccagcat cgccggtagt ttcagcggcg tgttattgcc gagaagattt
    27001 tttgccagca ccatcgcgct aagttgaatc ggctgcagga atggcagtac ctgaccgttg
    27061 atttccgcgc aatcgcccag cgcataaata tcgtcattac tggtttgcag ataactatcg
    27121 acgcaaacgc cgcgattaat cgtcagcccg gcgcgtcgtg ccagggctgt ttccgggcgc
    27181 agtccggtgg cggcaattac cgcatccact tcgatactac gctggtggtc cagcgttgcc
    27241 agaatgccag aatccgtttt ttccagcccc tgcaactgag atttcaacag cagatgaacg
    27301 cccatctccg tcaaccgatg ctgcaagcgg ctgcttactt ctggtggcat taatgacgcc
    27361 agaatactgg cagcgttgtc gattagcgtg accgctttgc ctgcacgaca aaaatccatc
    27421 gccagttcgc taccaatcaa accaccgccg acaatcaaca cgcgtcgggc atcccgcagt
    27481 tgcgtttcac aggcgcgata ctcttgctga ctatttaacg tcagcattaa ctcacgccca
    27541 ggcactggcg ggacaaaggc actggcacca gttgccagca ccagcttgtc gtattgccac
    27601 tgattattct ggcttttcac cacatgggct tcggcatcga tatccgtcac ccaggtgtgc
    27661 ggaaacaggt gcagattaaa ctgcccggca aattcacccg ccgtctggcg ggtaaggtcg
    27721 tcggcacgtt gcccctggct gataacatgg ctgaggtccg gtttgttgta ctcatccatg
    27781 ctgtcggcgg cgatcagggt taatggaata ctggcgtcct gtttgcgaat atttttcacc
    27841 agttggcggg cggcgaagcc cgaaccaatg atcacaatgc cgttgctcat tttgcctccg
    27901 atgccagttc gtcaaagacg tctttgccga gagagcattc cgggcagagg aagttatccg
    27961 gaacttcact ccacggcgtt cctggcgcaa cgtcctgcat tggctcgcct tttgccggat
    28021 cgtaaatcca ctggcagacg ctgcactgca tccgtgggcc gaggtcggac ttcgtggtgg
    28081 cagaggtttc ttctttaacc accgtattca ccgtgctctg cggcagcggc gcgagcgccc
    28141 actgacgggc gatttcgcga ccatgttcac ggcataactc cagagcgtcc tggtctggtc
    28201 gccatttggc tttcaggcta agcgacattt cgaaacccgc atcctgcagg cgcgtggaaa
    28261 gacgatccac cgcaccgccg ctccagccgt gagagccgaa agcactggcg cgtttgttac
    28321 ggaagcgtaa tccggtcatc tcctccacca gaccggcgat tttcggcatc atcacgttat
    28381 tcatcgtcga agtgccgacc agcacgcctt ttgagcggaa gacattggtc aggatttcgt
    28441 ttttatcgct tcgggcgacg ttgaaaattt tcaccgccac gcgtgggtcg gtttccgcaa
    28501 tcccctgggc gatagcgtca gccatcatgc gggtgttatt cgacatggtg tcgtagaaaa
    28561 tggtgattct gtcttcctga taatcagccg cccatttcag gtacagctcg acaatttgcg
    28621 tcgggttatc gcgccatacc acgccgtgag aggtcgctat catatcgact ggtaagttaa
    28681 agcccaggat ctcggtaatt ttcggtgtta ccaggcggct gaacggcgtc aggatattgg
    28741 cgtagtaacg ctggcactgc tcgaaaagct ccgtctgatc cacttcatcg ttgaacagat
    28801 gctcgtcgca gtagtgctga ccgaaagcat cgttactgaa cagcaccgcg tcgcctgtca
    28861 ggtaagtcat catgctgtcc ggccagtgca gcattggcgt ttcgacaaaa atgagctgtt
    28921 tgccgttgcc gatatccagc gtgtcaccag ttttcaccac attaaaattc cactccggat
    28981 gatgatgatg accatttatc gagtcgatag cgttggcagt acagtaaatc ggcgtatcgg
    29041 gaatttgcgc catcagttcg gtcagcgccc cagcgtggtc ctcttctgca tggttaatca
    29101 cgatgtaatc gatatccgcc agatcgattt cattacgcag gttctgcaca aattcgcggc
    29161 tgaatttatg gtcgacggtg tcgatcagca cgtttttttc ttcgcggatg aggtagctgt
    29221 tgtaactgct gccgcgtagc gttttatatt ccgtaccgtg aaaatcacgc acttcccagt
    29281 cacgttgacc aacctaatga atgttatttt tcaccacaat agacatagca acctcaattt
    29341 attcagcgtg ttctaaaaag atgtcttgct aattgcagat tacgtgccag ttttttatct
    29401 gattgttttt actctataaa taaaattatg ctcattaatg agtagtcaaa atgactatca
    29461 atcaaatggt cgatatgaca atatctatag tcaaattgac agtgaggcaa agatgagttt
    29521 ttccgttgat gtgctggcga atatcgccat cgaattgcag cgtgggattg gtcatcagga
    29581 tcgttttcag cgcctgatca ccacgctacg tcaggtgctg gagtgcgatg cgtctgcgtt
    29641 gctacgttac gattcgcggc agtttattcc gcttgccatc gacggtctgg caaaggatgt
    29701 actcggtaga cgctttgcgc tggaagggca tccacggctg gaagcgattg cccgcgccgg
    29761 ggatgtggtg cgctttcccg cagacagcga attgcccgat ccctatgacg gtttgattcc
    29821 tgggcaggag agtctgaagg ttcacgcctg cgttggtctg ccattgtttg ccgggcaaaa
    29881 cctgatcggc gcactgacgc tcgacgggat gcagcccgat cagttcgatg ttttcagcga
    29941 cgaagagcta cggctgattg ctgcgctggc ggcgggagcg ttaagcaatg cgttgctgat
    30001 tgaacaactg gaaagccaga atatgatgcc aggcgatgcc acgccgtttg aagcggtgaa
    30061 acagacgcag atgattggct tgtcccctgg catgacgcaa ctgaaaaaag agattgagat
    30121 tgtggcggcg tccgatctca acgtcctgat cagcggtgag acgggaaccg gtaaggagct
    30181 ggtggcgaaa gcgattcatg aagcctcgcc acgggcggtg aatccgctgg tctatctcaa
    30241 ctgtgctgca ctgccggaaa gtgtggcgga aagtgagttg ttcgggcatg tgaaaggagc
    30301 gtttactggc gctatcagta accgcagcgg gaagtttgaa atggcggata acggcactct
    30361 gtttctggat gagatcggcg agttgtcgtt ggcattgcag gccaagctgc tgagggtgtt
    30421 gcagtatggc gatattcagc gcgttggcga tgaccgtagt ttgcgggtcg atgtgcgcgt
    30481 gctggcggcg actaaccgcg acttacgcga agaggtgatg gcagggcgat ttcgcgctga
    30541 cttgtttcat cgcctgagcg tgtttccact ttcggtgccg ccgctgcgtg agcggggcga
    30601 tgatgtcatt ctgctggcgg ggtatttctg cgagcagtgt cgtttgcggc tggggctctc
    30661 ccgcgtggta ttaagtgccg gagcgcgaaa tttactgcaa cactatcgtt ttccggggaa
    30721 cgtgcgcgaa ctggaacatg ctattcatcg ggcggtagtg ctggcgagag ccacccgcaa
    30781 cggcgatgaa gtgattcttg aggcgcaaca ttttgctttt cctgaggtga cgttgccgcc
    30841 gccagaagcg gcggcggtgc ccgttgttaa gcaaaacctg cgtgaagcga cagaagcgtt
    30901 ccagcgtgaa actattcgcc aggcactggc acaaaatcat cataactggg ctgcctgcgc
    30961 gcggatgctg gaaaccgacg tcgccaacct gcatcggctg gcgaaacgtc tgggaatgaa
    31021 ggattaaata atcccggcct gatagaaatc ctgcaggtta attgcgccgg tgagtttgcc
    31081 gttttcatcc accaccggtg cggcagtgat tttgcgcttc atcaggatct ctttggcgtc
    31141 gatggcgcga ctttgcgcct gtaacgtggt gccgcctgtc gtcatcgctt cattaactgg
    31201 cgtggtgagt gcgccgccgc ctaccagcca gcgacgtaaa tcgccgtcgg taaagacgcc
    31261 ttgtacctgt tgttgagcgt cacataccgc caccagcccc agaccggtgc ggctgagttc
    31321 cagcatcgca tccatcacgc tggcggttaa cgccacctgt gggatggcat cgtcacggcg
    31381 catcagatga tgcactttat tcagcaagcg agcgcccagt gccccggctg ggtgggagcg
    31441 ggcaaaatct tcttcattaa atccgcgcgc ctgcatgacc gccatcgcca gcgcgtcacc
    31501 catcatcagg gtattgacgg tgctggaggt cggcgcaagg tgcatcgggc aggcttcgcg
    31561 ttctacggag atatccagca ccgcttttgc cgccaggccc agcggtgacg tcggtttgcc
    31621 ggtcatcgcc agcagcgcga tagatttatc ttccagacgc ggaataatca gatccagttc
    31681 cttcgcgcca ccggagtaag agataaacag catcacatcg cggctttcaa tcatccccag
    31741 atcgccgtgc agtgcttctg ccggatggac aaaaaaagcc gatgtgccgg tactggcaag
    31801 tgttgcggcg attttcttac caatgtggcc cgatttgcca attcccgaaa ccaccacttt
    31861 gccttcgcag tgcaggatga tattggcggc gcgaacaaaa tcatcgccca gacgttccgg
    31921 taaacggctt gcttcctgca actccagcat taacgtctga cgtcccgcgt tcagtagtgc
    31981 ttcactcatt gctctctccg gttatgatca catcgatccc tttctcttcc agcgcctgac
    32041 gaaacgccgg atcgatacct gcgtcggtaa tcagcttatc gacgctttca agactgcaaa
    32101 ctacgttggg gcttttacgg ccaaactttg atgagtccgc catcaaaatc acttcgcgcg
    32161 cggcattgca cattgcctta ctgacggtat aaacctcgtt aaaggtggtt acgcccgcat
    32221 tgagatcgat gccgtcggtg cccataaaca atttatcgaa actgaaatgt tcgaaggcat
    32281 tctctgccag ctgtccgtga aatgaggccg attttttacg aaacgttccg cctggcatca
    32341 ggatagtttg ttcgttatcc agttcagata gcgcattgac gatatgcaga ctgttggtca
    32401 tcaccgtgat gttattaaag cgcgagagca ggggaaccat ctgcaaaacg gtactgccag
    32461 catcaagaat gatcgaatcg ccatcatgaa taaaactaac ggcagcttct gctatcagct
    32521 ctttcttgtg ggtgttgatg agtgttttat gatcgatagg cggatcggat tcctctttat
    32581 tcaacaccac tccgccataa gtacgaatga cggttcctgc atgttccaga atgaccagat
    32641 ctttgcgaat ggttgtgcct gtggtgtcaa agtattgcgc caattcttca accgagcatt
    32701 taccctgctt ttgcagatac tccagaatgg cggcctgacg ctgacgaggt ttcatgggcg
    32761 tgattacctg atttaggttt caaaatgata acttcgcaag caagtaacat tcacaacgaa
    32821 attatccacg tttcagttta agcgccaatg atagagcatt ctgtgatagt ggatcatggg
    32881 gaaagatcac atcgggctgt aaatcacccg catgcatacc ggtaattgcg ggaatttttt
    32941 gcggtcgtgc gaagacggtc agtcctttca taaacaaggt gtcgacgatg cgctgctgat
    33001 cgtccagggc gatggcgacc acgacccgtg gtttaaagcg cccgctggaa cggcccacgc
    33061 caacccgccc ttgctggcat agtgtgtcga aggcacggtt aaaacgagaa atttgccagc
    33121 cgcctaaggc cagttgcgca caccaggcga taacggcgac ggtgatgagt gcggatacca
    33181 taacttttcc ttaatgtagt ggcggaagtc ctgtatgcct gatgagcgta gcgcatcagg
    33241 cattctatta gtctcacgga gatccttaac ggatctccgc tgttgattac aacatcacct
    33301 gaccgccggt gacattgatc gactgtccgg tgcagtacga cgccttagga ctggcataga
    33361 gcagcagcat attcagcaca tcctgataat cgcagccgcg tttgagcggt actttgtcga
    33421 tgtaatactg ctcgacttga tccggtttga tacccagctt ggtcgcgtat tggggtaaca
    33481 gtgactggaa cataggcgat ttcagcaggt taccgagcat cagtgaatgc accgtaatgc
    33541 cgtactccgc cagatccagc gccagtgatt gagtcagccc gacgccacca aatttcgctg
    33601 cgctgtagcc agagttgtgt ttgctgccca ctttgccgga tttcgagttg atctgaataa
    33661 tgcgaccctg aatcccgtcg cggatcatca aacgagaaaa ttcacgggca cacaggaaat
    33721 aacccaccag attcacggtc aaaatcgccg agctggaagt cgctgataaa ggctgctttt
    33781 gctattccgg cgctgtagac cagcaaatcc acgcgcccaa agatttcatc taccccacga
    33841 gagagcgcca ggacgctttg ctcgctggtg gcgtcagcac caaaactata cgctgttcct
    33901 tcaccatatt cggcgttaat ttcttgtgcc acatttgcgg ctttgtcgct ctgaatatcg
    33961 acaaccgcga cgcgataccc ctcggcagcc agaccgtggc acaggaacgc gcctaaggtt
    34021 tgcccaccac cgatgacaac ggcaacctga ttcatttttt actccttaac agattcaaac
    34081 ttcaaaaccg atcccggcgc gatatcgtcg ggtacagggc ctgccacatg gacagtgccc
    34141 ggaaattccg cttcgtttaa accatcgaag cgcagggtga catgacccag ttcgcgaagg
    34201 ttgtcttccg ccacgctgcc aacagcggtc accggatagc gatgctgccc gagcgtaaat
    34261 tgcaaaccgg gatggagtgc acctttcaac tcgccgtggc aatgaatgaa gcaatactct
    34321 tcgaggtccg ctggcgcgcc ttcacgaaag gtgatgagca tctggtcgct gagggcgtca
    34381 gtggcactcg cgccgatacg ggtgatggtg gtctgataaa taacggtcat gtttcagcct
    34441 ctattgatag ataaaaccgg agacaaacca ggcgatcagt acagtcggtg cgccggttaa
    34501 aaagtggctc accagtacag aagggacacc gacgcgaacc gtgtcctgac gggcttccgc
    34561 cagcgacaaa ccgaccggga tgaagtcgca ggccgcctgc gcgttgatgg caaacagtgc
    34621 cggtaaagcc agatgcggcg gaatattgcc gagaccaatc tgcacgccaa tcaatacgcc
    34681 gataacctgt gcgataactg cgcctgggcc gaggaaaggt gaaagcagcg ggaaggagca
    34741 gatgagcgcc agcatgacca gacctagtgg atggctcgcc agcggagcaa gaccgtgggc
    34801 aatccagtca ccaaggccag aagccataat gatgccaatg agcgccgaga cgaacgccat
    34861 aaacggcaga atggttttta atacagtgtc gatggtgtca cgaccagatt gaaacaacac
    34921 ggcaacggca gaacccatac ccattcccac cttcgccagt aaaccatcgc tttgttcggt
    34981 gattttcttg ctggtgtcat agtcacggcc cacggaagag ggttgtggtg tcgcatcacc
    35041 cactacagtg atgttttctt cttttacgcc agagacataa atatcttcca caatgtactg
    35101 cgccagcgga ctggacttgc ccgtcgagtg gatattaatg gtgggaatac gtcgtttcgg
    35161 atagatgccg cagcgtaatg tgccgccaca gtcgattacc gcgacaccaa tttccgcctc
    35221 cgcgggttca ccttctttaa atccgtcaat agcctgccag ccagtaagtt gtgccagttt
    35281 gtcaacaatc gcaggccggg taccggcggt gatatagacg atttttttgc ccggcgtggc
    35341 ttccagctca agcgggccgc cccagccacc cgttcctttt tcgatccgaa tacgcgtcat
    35401 gatgttgctc ctgccaggtg aactttttgt tcaagttgaa tgcccatctt tttctcaaaa
    35461 atggcggtgg tcagatcggt cacccagccg cgaaagaagt tggtgaccag accaaccagc
    35521 agatagctca ccgccagtgg gccaagtggc aggttcagcg ttgtcagacc gctggcaatg
    35581 ccaagataaa caaacagttc gccagggttg atatggggaa agaggccatt cattgagtgg
    35641 cagctataag aggctgccgc gtagtagctg ggtttgtact tttccggcat aaagcgaccc
    35701 aggcttaagg tcatcggatt gcaaaagaca aacgtgccaa tgcacggtag cagcaggtaa
    35761 cgggaaacag ggttaccggc gcaacgttga gcaaaacgtt caatacgatg ctgaccgata
    35821 aaattaatca gtgcgttcat gataaccagc aggctaatca acagcggaag aatgccggtc
    35881 accatcccgg taaacacctc tccgcccttt tggaacagcc cgataaacca ctctgcacca
    35941 tgagtaatgg tttctatcat tgttctctcc ttcaggattt attgttttat taccaaacgg
    36001 caacctaatc taatcagatt gaaagatttg aaagtgttat tttgatcgca aaatgaaaga
    36061 taaatatttt aatttgaaag tttgaataaa aggatagcga ggggaatgag ttgagttatg
    36121 taaagtccgt atcgggcagt gactaccgct tccttgtgcg gggcgtgatg ctttaccata
    36181 cttgcccctg gttgaatctg ttaaatggac ccctcatgtt caagcgtcgt tatgtaacat
    36241 tgcttcccct ttttgtgttg cttgccgcct gtagcagcaa gccaaaacct actgagactg
    36301 aaacgaccac cggaacgccg tctggcggct tcctgcttga gcctcagcac aatgtgatgc
    36361 agatgggcgg cgatttcgct aataacccga atgcccagca gttcatcgac agaatggtga
    36421 acaaacacgg tttcgatcgt cagcagttgc aggaaattct ctcccaggcg aagcgtctgg
    36481 attcggtact gcggctgatg gataaccagg caccaaccac atcggtgaaa ccgccatcag
    36541 gtccgaacgg cgcatggctg cgttatcgca aaaaatttat tacgccggac aacgtgcaga
    36601 acggtgtggt tttctggaat cagtatgaag atgcgttgaa tcgcgcgtgg caggtgtatg
    36661 gcgtaccgcc ggaaattatc gtcgggatta tcggcgttga aacccgctgg gggcgcgtga
    36721 tggggaaaac tcgcatcctc gatgcgctgg caacgctgtc atttaactac ccacgccgcg
    36781 cggagtattt ctctggcgag ctggaaacct tcctgttgat ggcgcgcgac gagcaggacg
    36841 atccgctcaa tctgaaaggt tcctttgccg gggcgatggg ctacggacag tttatgccgt
    36901 cgtcttacaa acaatatgcg gtagatttca gcggcgacgg gcatatcaac ctgtgggatc
    36961 cggttgatgc gatcggtagt gtggcgaact atttcaaagc gcacggctgg gtgaaaggcg
    37021 atcaggtcgc ggtaatggca aacggtcagg ctccaggctt gccaaatggc ttcaaaacca
    37081 ggtacagcat ttcgcagctt gccacggcag gtttaacgcc acagcagccg ctgggcaacc
    37141 atcaacaagc cagcctgctg cgtctggatg tcggtaccgg ttaccagtac tggtacggtc
    37201 tgccgaactt ctacaccatt acccgttata accacagcac ccattacgca atggcggtct
    37261 ggcagttagg acaagccgtg gcgctggcgc gcgtgcagta actatttgca agaggggggc
    37321 gtgagcttcc ctctggctaa tcagcttttt tctgaatcct cctcgtaaaa ttgcaacgcc
    37381 aacaccatct tcctgacgaa agtgctatct tgtccggcat aaatttttga ctgactgagg
    37441 ttgtgatgac tgacagtgaa ctgatgcagt taagcgaaca ggttgggcag gcgctgaaag
    37501 cccgtggcgc aaccgtaaca actgccgagt cttgtaccgg tggttgggta gcgaaagtga
    37561 ttaccgatat tgccggtagc tccgcctggt ttgaacgcgg atttgtcacc tacagtaacg
    37621 aagccaaagc gcagatgatc ggcgtacgcg aagagacgct ggcgcagcat ggcgcggtga
    37681 gtgaacccgt cgtggtggaa atggcgatag gtgcactgaa agcggctcgt gctgattatg
    37741 ccgtgtctat tagtggtatc gccgggccgg atggcggcag tgaagagaag cctgtcggca
    37801 ccgtctggtt tgcttttgcc actgcccgcg gtgaaggcat tacccggcgg gaatgcttca
    37861 gcggcgaccg tgatgcggtg cgtcgtcagg ctactgcgta tgcattgcag accttgtggc
    37921 aacaatttct acaaaacact tgatactgta tgagtataca gtataattgc ttcaacagaa
    37981 catattgact atccggtatt acccggcatg acaggagtaa aaatggctat cgacgaaaac
    38041 aaacagaaag cgttggcggc agcactgggc cagattgaga aacaatttgg taaaggctcc
    38101 atcatgcgcc tgggtgaaga ccgttccatg gatgtggaaa ccatctctac cggttcgctt
    38161 tcactggata tcgcgcttgg ggcaggtggt ctgccgatgg gccgtatcgt cgaaatctac
    38221 ggaccggaat cttccggtaa aaccacgctg acgctgcagg tgatcgccgc agcgcagcgt
    38281 gaaggtaaaa cctgtgcgtt tatcgatgct gaacacgcgc tggacccaat ctacgcacgt
    38341 aaactgggcg tcgatatcga taacctgctg tgctcccagc cggacaccgg cgagcaggca
    38401 ctggaaatct gtgacgccct ggcgcgttct ggcgcagtag acgttatcgt cgttgactcc
    38461 gtggcggcac tgacgccgaa agcggaaatc gaaggcgaaa tcggcgactc tcacatgggc
    38521 cttgcggcac gtatgatgag ccaggcgatg cgtaagctgg cgggtaacct gaagcagtcc
    38581 aacacgctgc tgatcttcat caaccagatc cgtatgaaaa ttggtgtgat gttcggtaac
    38641 ccggaaacca ctaccggtgg taacgcgctg aaattctacg cctctgttcg tctcgacatc
    38701 cgtcgtatcg gcgcggtgaa agagggcgaa aacgtggtgg gtagcgaaac ccgcgtgaaa
    38761 gtggtgaaga acaaaatcgc tgcaccgttt aaacaggctg aatttcagat cctctacggc
    38821 gaaggtatca acttctacgg cgaactggtt gacctgggcg taaaagagaa gctgatcgag
    38881 aaagcaggcg cgtggtacag ctacaaaggt gagaagatcg gtcagggtaa agcgaatgca
    38941 actgcctggc tgaaagataa cccggaaacc gcgaaagaga tcgagaagaa agtacgtgag
    39001 ttgctgctga gcaacccgaa ctcaacgccg gatttctctg tagatgacag cgaaggcgta
    39061 gcagaaacta acgaagattt ttaatcgttc gttattgatg ataatagata atgtgtgcac
    39121 ggacggtccc ctcgcccctt tggggagagg gttagggtga ggggaacagg ccaacactgg
    39181 cgtgaaaaat tactctcacc tcgatcctct aacgaattgg ctgcacactc attgactatt
    39241 aaatctgttt atcagcaaaa cctgggtcgc atctgcggcc cttttgcttt tttaagttgt
    39301 aaggatatgc catgacagaa tcaacatccc gtcgcccggc atatgctcgc ctgttggatc
    39361 gtgcggtacg cattctggcg gtgcgcgatc acagtgagca agaactgcga cgtaaactcg
    39421 cggcaccgat tatgggcaaa aatggcccag aagagattga tgctacggca gaagattacg
    39481 agcgcgttat tgcctggtgc catgaacatg gctatctcga tgacagccga tttgttgcgc
    39541 gctttatcgc cagccgtagc cgcaaaggtt atggacctgc acgtattcgt caggaactga
    39601 atcagaaagg tatttcccgc gaagcgacag aaaaagcgat gcgtgaatgt gacatcgact
    39661 ggtgcgcact ggtgcgcgat caggcgacgc gaaaatatgg tgaacctttg ccaactgtct
    39721 tttcagaaaa agttaagatc cagcgttttc tgctctatcg tggctatctg atggaagata
    39781 tccaggatat ttggctaaat tttgccgact gaacgcatac ggtattttac ttcccactca
    39841 agaaaactta tcttattccc acttttcagt taccagcccg gcggttaaga cacgctggag
    39901 ctggtggcga tatttcgtta gcttgatttc aggataatta tgagcaagag caccgctgag
    39961 atccgtcagg cgtttctcga ctttttccat agtaagggac atcaggtagt tgccagcagc
    40021 tccctggtac cccataacga cccaactttg ttgtttacca acgccgggat gaaccagttc
    40081 aaggatgtgt tccttggact cgacaagcgt aattattccc gcgctaccac ttcccaacgc
    40141 tgcgtgcgtg cgggtggtaa acacaacgac ctggaaaatg tcggttacac cgcgcgtcac
    40201 cataccttct tcgaaatgct gggcaacttc agcttcggcg actatttcaa acacgatgcc
    40261 attcagtttg catgggaact gctgaccagc gaaaaatggt ttgccctgcc gaaagagcgt
    40321 ctgtgggtta ccgtctatga aagcgacgac gaagcctacg aaatctggga aaaagaagta
    40381 gggatcccgc gcgaacgtat tattcgcatc ggcgataaca aaggtgcgcc atacgcatct
    40441 gacaacttct ggcagatggg tgacactggt ccgtgcggcc cgtgcaccga aatcttctac
    40501 gatcacggcg accacatttg gggtggccct ccgggaagtc cggaagaaga cggcgaccgc
    40561 tacattgaga tctggaacat cgtcttcatg cagttcaacc gccaggcaga tggcacgatg
    40621 gaaccgctgc cgaagccgtc tgtagatacc ggtatgggtc tggagcgtat tgctgcggtg
    40681 ctgcaacacg ttaactctaa ctatgacatc gacctgttcc gcacgttgat ccaggcggta
    40741 gcgaaagtca ctggcgcgac cgatctgagc aataaatcgc tgcgcgtaat cgctgaccac
    40801 attcgttctt gtgcgttcct gatcgcggat ggcgtaatgc cgtccaatga aaaccgtggt
    40861 tatgtactgc gtcgtatcat tcgtcgcgca gtgcgtcacg gcaatatgct cggcgcgaaa
    40921 gaaaccttct tctacaaact ggttggtccg ctgatcgacg ttatgggctc tgcgggtgaa
    40981 gacctgaaac gccagcaggc gcaggttgag caggtgctga agactgaaga agagcagttt
    41041 gcccgtactc tggagcgcgg tctggcgttg ctggatgaag agctggcaaa actttctggt
    41101 gatacgctgg atggtgaaac ggctttccgt ctgtacgaca cctatggctt cccggttgac
    41161 ctgacggctg atgtttgtcg tgagcgcaac atcaaagttg acgaagctgg ttttgaagca
    41221 gcaatggaag agcagcgtcg tcgcgcgcgc gaagccagcg gctttggtgc cgattacaac
    41281 gcaatgatcc gtgttgatag tgcatctgaa tttaaaggct atgaccatct ggaactgaac
    41341 ggcaaagtga ccgcgctgtt tgttgatggt aaagcggttg atgccatcaa tgcaggccag
    41401 gaagctgtgg tcgtgctgga tcaaacgcca ttctatgcgg aatccggcgg tcaggttggt
    41461 gataaaggcg aactgaaagg cgctaacttc tccttcgcgg tggaagatac gcagaaatac
    41521 ggccaggcga ttggtcacat cggtaaactt gctgcgggtt ctctgaaagt gggcgacgct
    41581 gtgcaggctg atgttgatga ggctcgtcgc gcccgtattc gtctgaatca ctccgcaacg
    41641 cacctgatgc acgctgcgct gcgccaggtt ctggggactc atgtatcgca gaaaggttca
    41701 ctggttaacg acaaggtgct gcgcttcgac ttctcacaca acgaagcgat gaaaccggaa
    41761 gagattcgtg cggtcgaaga cctggtgaac gcacagattc gtcgcaattt gccgatcgaa
    41821 accaacatca tggatctcga agcggcgaaa gcgaaaggtg cgatggcgct gttcggcgag
    41881 aagtatgatg agcgtgtacg cgtgctgagc atgggcgatt tctccaccga gctgtgtggc
    41941 ggtactcacg ccagccgcac tggtgatatt ggtctgttcc gcatcatctc tgaatcgggt
    42001 actgctgcag gcgttcgtcg tatcgaagcg gtaaccggag aaggcgctat caccaccgtt
    42061 catgcagaca gcgatcgctt aagcgaagtc gcgcatctgc tgaaaggcga tagcaataat
    42121 ctggcggata aagtgcgctc agtactggaa cgtacgcgtc agttagaaaa agaactacaa
    42181 cagcttaaag aacaagctgc cgcacaggag agcgcaaatc tttccagtaa ggcaattgat
    42241 gttaatggtg ttaagctgtt ggttagcgag cttagcggtg ttgagccgaa aatgttgcgt
    42301 accatggttg acgatttaaa aaatcagctg gggtcgacaa ttatcgtgct ggcaacggta
    42361 gccgaaggta aggtttctct gattgcaggc gtatctaagg acgtcacaga tcgtgtgaaa
    42421 gcaggggaac tgattggtat ggtcgctcag caggtgggcg gcaagggtgg tggacgtcct
    42481 gacatggcgc aagccggtgg tacggatgct gcggccttac ctgcagcgtt agccagtgtg
    42541 aaaggctggg tcagcgcgaa attgcaataa tataagcgtc aggcaatgcc gtggactcgc
    42601 ttcacggcat tcgcattaac gctatcgaca acgataaagt caggttgaag ttgtgtatat
    42661 cggctaaact taggtttaac agaatgtaat gccatgactg cttacatgta atgtgtttgt
    42721 cattgcttac tttttggcgt tatatgatgg ataatgccgg gatacagaga gacccgactc
    42781 ttttaatctt tcaaggagca aagaatgctg attctgactc gtcgagttgg tgagaccctc
    42841 atgattgggg atgaggtcac cgtgacagtt ttaggggtaa agggcaacca ggtacgtatt
    42901 ggcgtaaatg ccccgaagga agtttctgtt caccgtgaag agatctacca gcgtatccag
    42961 gctgaaaaat cccagcagtc cagttactaa tctttccgcg tctcatcttt atcggtgaga
    43021 cgcaccctca aaatttctcc ttcactctat agtcttttcg ctttactccc gttcattcaa
    43081 cttaagtctc catttttttg cattactgct atctgccaga cctccgtttt tctgtagata
    43141 aaacactctt tttgacgttt ttacagacta attgagcgtg aagtgtgcaa acgataaaag
    43201 tgtgggaaaa attgtttgac ttataagtct cagaaagtaa tatgtgcgcc acgcagcgac
    43261 gatgagcgat aaacaagttc ttcgaagcac tcgtaagagg cgtgtggtga ggtggccgag
    43321 aggctgaagg cgctcccctg ctaagggagt atgcggtcaa aagctgcatc cggggttcga
    43381 atccccgcct caccgccatt tgcatccgta gctcagctgg atagagtact cggctacgaa
    43441 ccgagcggtc ggaggttcga atcctcccgg atgcaccatc tcttacttga tatggcttta
    43501 gtagcggtat caatatcagc agtaaaataa attttcccga tgcatccgta gctcagctgg
    43561 atagagtact cggctacgaa ccgagcggtc ggaggttcga atcctcccgg atgcaccata
    43621 ttctccgaaa tcttcagcaa tgaaggtatc gaagaggtag cggaattaac cgcaggcacc
    43681 agggatgata acgttgcttt agcaacggcc cgaagggcga gccgcaaggc gagtaatcct
    43741 cccggatgca ccatctctta attgatatgg ctttagtagc ggtatcaata tcagcagtag
    43801 aataagtttt cccgatgcat ccgtagctca gctggataga gtactcggct acgaaccgag
    43861 cggtcggagg ttcgaatcct cccggatgca ccatattctc cgtaaccttc agcaatgaag
    43921 gtatcgaaga gatagcggta ttaaccgcag gcaccaggaa ggataacgtt gcgccagcaa
    43981 cggcccgaag ggcgaacgtc agtgagtcat cctcccggat gcaccatctt ctccaaaaaa
    44041 tttccttcaa aaatatctca tttctaaata actcctttat ccatttcctg aattttcttc
    44101 tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa gtagcgtcat atttttcgac
    44161 ttgcgagaag accatgtacg agcgttatgc aggtttaatt tttgatatgg atggcacaat
    44221 cctggatacg gagcctacgc accgtaaagc gtggcgcgaa gtattagggc actacggtct
    44281 tcagtacgat attcaggcga tgattgcgct taatggatcg cccacctggc gtattgctca
    44341 ggcaattatt gagctgaatc aggccgatct cgacccgcat gcgttagcgc gtgaaaaaac
    44401 agaagcagta agaagtatgc tgctggatag cgtcgaaccg cttcctcttg ttgatgtggt
    44461 gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg gggagtgaaa gcgccatcgc
    44521 tgaggcattg ctggcgcacc tgggattacg ccattatttt gacgccgtcg tcgctgccga
    44581 tcacgtcaaa caccataaac ccgcgccaga cacatttttg ttgtgcgcgc agcgtatggg
    44641 cgtgcaaccg acgcagtgtg tggtctttga agatgccgat ttcggtattc aggcggcccg
    44701 tgccgcaggt atggacgctg tggacgttcg cttgctgtga gtgaagcgtt atcgcttttc
    44761 tcgttgtttg ccagtagttt tctcagcgct acactattac ccggcaactc cgaagtcgtg
    44821 ctggtggcaa tgttgctttc cgggatcagt catccctggg ttttagtttt aacagcaaca
    44881 atgggtaata gccttggagg gttaactaac gttatccttg ggcgtttctt tccattgcgt
    44941 aaaacatcgc gctggcaaga gaaagctatc ggctggctga aacgctatgg tgcagtcacg
    45001 ctattattaa gctggatgcc cgtggttggc gatttactgt gtctgttagc gggatggatg
    45061 cgcatctcgt ggggaccggt aatctttttt ttgtgccttg gtaaagcgtt acgctatgtt
    45121 gcagttgcag cagcgaccgt tcagggcatg atgtggtggc actaattgta ggcctgcaca
    45181 tatggtcacc attacagtta tgctaattaa aacgattttg acaggcggga ggtcaatttg
    45241 atcccggacg tatcacaggc gctggcctgg ctggaaaaac atcctcaggc gttaaagggg
    45301 atacagcgtg ggctggagcg cgaaactttg cgtgttaatg ctgatggcac actggcaaca
    45361 acaggtcatc ctgaagcatt aggttccgca ctgacgcaca aatggattac taccgatttt
    45421 gcggaagcat tgctggaatt cattacacca gtggatggtg atattgaaca tatgctgacc
    45481 tttatgcgcg atctgcatcg ttatacggcg cgcaatatgg gcgatgagcg gatgtggccg
    45541 ttaagtatgc catgctacat cgcagaaggt caggacatcg aactggcaca gtacggcact
    45601 tctaacaccg gacgctttaa aacgctgtat cgtgaagggc tgaaaaatcg ctacggcgcg
    45661 ctgatgcaaa ccatttccgg cgtgcactac aatttctctt tgccaatggc attctggcaa
    45721 gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga aaatttctgc gggctatttc
    45781 cgcgttatcc gcaattacta tcgtttcggt tgggtcattc cttatctgtt tggtgcatct
    45841 ccggcgattt gttcttcttt cctgcaagga aaaccaacgt cgctgccgtt tgagaaaacc
    45901 gagtgcggta tgtattacct gccgtatgcg acctctcttc gtttgagcga tctcggctat
    45961 accaataaat cgcaaagcaa tcttggtatt accttcaacg atctttacga gtacgtagcg
    46021 ggccttaaac aggcaatcaa aacgccatcg gaagagtacg cgaagattgg tattgagaaa
    46081 gacggtaaga ggctgcaaat caatagcaac gtgctgcaga ttgaaaacga actgtatgcg
    46141 ccgattcgtc caaaacgcgt tacccgcagc ggcgagtcac cttctgatgc gctgttacgt
    46201 ggcggcattg aatatattga agtgcgttcg ctggacatca acccgttctc gccgattggt
    46261 gtagatgaac agcaggtgcg attcctcgac ctgtttatgg tctggtgtgc gctggctgat
    46321 gcaccggaaa tgagcagtag cgaacttgcc tgtacgcgcg ttaactggaa ccgggtgatc
    46381 cttgaaggtc gcaaaccggg cctgacgctg ggtatcggct gcgaaaccgc gcagttcccg
    46441 ttactgcagg tgggtaaaga tctgttccgc gatctgaaac gcgtcgcgca aacgctggat
    46501 agcattaacg gcggcgacgc gtatcagaaa gtgtgtgatg aactggttgc ctgcttcgat
    46561 aatcccgatc tgactttctc tgcccgtatc ttaaggtcta tgattgatac tggtattggc
    46621 ggaacaggca aagcgtttgc agaagcctac cgtaatctgc tgcgtgaaga gccgctggaa
    46681 attctgcgcg aagaggattt tgtagccgag cgcgaggcat ctgaacgccg tcagcaggaa
    46741 atggaaaccg ctgataccga accgtttgcg gtgtggctgg aaaaacacac ctgacagaaa
    46801 agaaaaaggc cactcgtgag tggccaaaat ttcatctctg aattcaggga tgatgataac
    46861 aaatgcgcgt ctttcatata ctcagactcg cctgggaaga aagagttcag aaaattttta
    46921 aaaaaattac cggaggtggc taaatgccgt tgttagatag cttcacagtc gatcataccc
    46981 ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa caccccgcat ggcgacgcaa
    47041 tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca gaaagaggga
    47101 tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac ggtaatggtg
    47161 tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg agtctgattg
    47221 gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa gacgtgctga
    47281 aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc acttaccaga
    47341 tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt gacgtacgca
    47401 tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg cacatctagt
    47461 cagtaaacta tcttcacaat taattgaaaa aagccagttc aaatgaactg gcttagttgt
    47521 acttagtgcg caccgccgcc accgccacct gcgccaaatg gcggtttagc aaaccacacc
    47581 agccccagca gtacgaggaa tatcccggct gacatccaga agatctcatt ggcggaaata
    47641 atcagcccct gattggtgat ctgctgggca atccagcctg atgcctgctg ttgcgtcatc
    47701 ccaagccctt ccagttgact gtacatcgcc tgggcattcg ggttgaacgg gtttaccgac
    47761 tcagtcaact gcgcatggtg catcgactcg cggttggtcc acatggtcgt ggttatcgac
    47821 gtgccgatag accccgccag cgttcgcgta aagttagaga ggctcgatgc cgccgccagt
    47881 cgttccggtg gcaaaccaga cagcgtaatg gtggtcagcg gcataaagaa gcaggccacc
    47941 gcaaacccct ggataaactg cggccaggcc gacgcgccaa aatccatacc tggttcaaag
    48001 gtataggcac gccagtagaa gcagacggca tacataataa agctgaaggt taccagccgc
    48061 cgcatatcca gtttatgcgc gaagcggccg ataatcggcg acaggatcac cggaataatc
    48121 cctaccggcg cagaggccaa acctgcccag gtcgccgtgt aaccgtagac ctcctgcaac
    48181 aactgcggca gcagaacaat agcgccgaag tagagcatat acgcgaggct gatacacaag
    48241 cagccgatgg tgaagttgcg cgacttaaac aacgacagat cgactatcgg gttatcgtcg
    48301 gtcagctccc agacaatcag gaagcagata gccaccaccg ccaccacggt aaggatgata
    48361 atttcctgtg atgaaaacca gtccagctct ttaccgcggt cgagcataat ctgcaggctg
    48421 ccgataccaa taaccagcag tgccagcccc acggcatcaa tccgccgccg tttggtgcgg
    48481 gtttcacgtc cgcgcagagt ttgcagtgtc atcaacacca ccgccacgcc aatcggcacg
    48541 ttgatgaaga atatccagcc ccagtggtaa ttatcgctga tataaccgcc gaggatcggg
    48601 ccgcaaattg gcgcgacaat caccgtcatc gaccacaacg ccagcgcgat cgagcgtttg
    48661 gctggcgggt agttattcag caatagactt tgcgaaagcg ggatcaacgg cccggcgaca
    48721 atcccctgaa tcacgcggaa gaagatcagc atattcaggc tgctggagac accacacgcc
    48781 cacgacgcaa tagcaaaggc gatggtggac caaaggaaca gtttcacttc cccgacgcgc
    48841 tttgccagcc agccggtaag cgggatcgag atggcattcg ccaccccgaa agaagtgatt
    48901 acccacgttc cctggctgag cgatgagccc agattcccgg cgatagtggg gatcgccacg
    48961 ttagcaatgg tggagtccag cacctgcatg aatgtcgcca gtgacagcgc aatcgtcata
    49021 atgaccagtt gcgcgccttc cagcggtttt tgctgttgca tcacacgcac ctctggatta
    49081 gccagcgtta gcttttacga tatcgtcgat cagtttattg acaggtgcca ggctgatttc
    49141 acgcgcggta cttaccgcga ccggtgttga acgtacttta tttgccagta cctgaccgtc
    49201 acggttagtg gtattgacgc tcaccagtgt ggacaaacca atgcgcagcg ggtattgctc
    49261 cagctgtttc tggtccagtt cgatacgcac aggtagacgc tgaacgactt tgatccagtt
    49321 accggtcgca ttttgcgccg gaagcagtga gaatgcgcta cctgtgccca tatccagacc
    49381 aaccacttta ccggtgtatt tcacatcatc gccgtaaata tccgtggtga tagtgaccgg
    49441 ctgaccgata cgcatattgg caatctgcgt ctctttaaag ttggcatcca cccacatatt
    49501 ggtggctgga acgaccgcca tcagcggcgt cgttgggcta atttgcgccc caggctgtac
    49561 cgcacggcgg gagacataac cggtcatcgg actgacaata cgggtacgct ccagcgccag
    49621 ccaggcgtta cgtacttcgg tggcagcctg ttgcacggca ggctgatctt ccagtttagt
    49681 ccccagaatc atcgcctgat tggcattgta ttgttgaata gcgacgtcca gttgcgcctg
    49741 ggcactggtg acggcgtcgc gggcgtgttg cagctcttcg cgaccaatca ggttggcatt
    49801 gcccagcggc acacggcggt tgtagtcgct ttgtgctttt gcgagggcga ttttctgcac
    49861 ctcaatattc gcctgcaact gtttgctgtt aatcatcagc tggtgggttt ggcgaacgct
    49921 ggaagccagt gcagttttgg ctttttcaaa cgcctggcga gcatctgtcg ggtcgagagt
    49981 gaccagcacg tcgccttctt ttacaaaatc ggtgttatcg gcccagactt tcgtcacgct
    50041 gccagacacc tgagacataa tttgaatctg attccctgcc acgtatgcgt catcggtttc
    50101 ttcgaaatga cgcagtacca aaaaccaata aatccctatc gctacggcaa taattataaa
    50161 gagcaaggtg agaaggagga gcagacgctt acgtttgccg ctcttcttta ccggttgctg
    50221 cggggtttga gtctccgcat ttgcgctcat attgttctcc acgatcttct taactcatcg
    50281 gctgagccga cctgcttgcc aaaaaggcca gcatgttgcg atgctggccg gtcatttttt
    50341 cttttataaa tctggatttt tgagcgagat gacgcgttag ctcatcgctt cgagaaccac
    50401 accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt gctcgagctg
    50461 atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat gctgcggtgg
    50521 taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca ggcagcggcg
    50581 atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat cggcaatacg
    50641 cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct gaatactgtg
    50701 gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa tcccctgagc
    50761 cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc aaagacgagt
    50821 cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt ttagcatttg
    50881 ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc agccgatata
    50941 gtaacgacag tgacaaataa agtaaatgta ttgttttaga aaaatgattc ttgtgggtta
    51001 tataatcgcc atcactttcc aggcgagccc ataggccagc gcactaagca gtgttgggat
    51061 gataatgctg cgtgttttat agaaactggc acccagtacc gcgaagccga ccagcgtggg
    51121 cacgaaacgg cgtgtatcgt gcatcacttc tggtgcggta gagacaacca gcagagcgca
    51181 tatcgaggcg atgccaatgg tgtcgagcaa aatacctacc gcgccacgtt tggttgggcg
    51241 ggcattaccc acacgcaggc gcagcggcaa atagcggaag caataattcg ccgcgccaac
    51301 cagtaaccca ggcagcagaa cctcatagct catcgggcgc tccttgccag aatgcttgga
    51361 ttaacgcagt gaggcagcca cagacaatgc ctgccagaat ggcgacggga atagaaaata
    51421 gcgttacgcc tgcaagggca ccaactaacg ctgcggtaac gcaaagagat tgtttgcgct
    51481 ggaaagaggc gagcaggaaa ctcataaaga gtgccggaag cataaaacct aatgctgctt
    51541 caacggcggg ataaccttgc agcaagccgc tgccagagaa tgcccctatt accgtaccaa
    51601 atacccacga tgaccatgaa ctgaaggcaa tgccgatcat ccagttctcg ctccagcggc
    51661 gattattgcg taccagtttt gctgttgcgg cggcaaaaac ctcatccgtc aggccaaacg
    51721 cccacagggc ggttttcgat ttttgcagac gctgaataat acggctacgc agtgacgggc
    51781 catacaacac atggcgaaca tccattgcca tgacggtcag tgcagcaacc cacaaactac
    51841 tcccggctgc cagcatcgcg gtaatgacga actggctcgc gcctgcataa atgatgcagg
    51901 agaaaaaaac gctttcgaga ggagagaatc ccagacgggt cgcattcaga ccgaacgcaa
    51961 aggccaccgg aatataacta ataacaatcg gtaaactgtc tttgcatcct tccatgaagg
    52021 tcgccgaacc aggagcaggc tgtggagtag ggctttccat agagtgtacg cttaacgatt
    52081 gttaatatta taaatagact gaatgaatat cttaacctta tcagactgat gggcttctta
    52141 acacccttat aagtgtaaag ccacgaaaac ggttgctgat tgcttaattt gcggcttcct
    52201 gacgatgaaa acctcgccac cagaccagta agttaactag ggcaatcgtc gcgccagcca
    52261 gacaaacgcc agcccaaccg ccatgttgcc aggctgaggc tgaaattagc gaaccggcgg
    52321 caccgccaat aaagtagctg gtcatgtaac ctgcggtcag gcgattacgc gcatcagggt
    52381 gtattcgata aattaccgtc tggttagtga tatgcacgcc ctgtacggtg agatccagca
    52441 ccaggatgcc gataatcagc gccagtacgg aagtgtgacc aaaccagatc gccagccatg
    52501 aaagtaatag cagcagcaga ccgaaagttg tggtgtggtg cgatttaccc ttatcggcaa
    52561 aaccgcccgc cggacgagcg cccaacgctc cggcagctcc cgcaagtcca aacagaccaa
    52621 tgacaccatc gctgtagtta aaaggtggag cggcaagcaa aaaggccatt gaggtccaga
    52681 gaatgctgaa gttggcaaag gtcaggcagc ccagcaatgc gcgggtgcgg aggatcttat
    52741 cgctgataaa catgctgaaa acggaaccta acaactgtgg gtagttgagg tgggtttctg
    52801 atttcatttg tggcagacca cgccataatg ctagcgccat cagtgccatt aacaccgagg
    52861 caacccaaaa gacggtgcgc cagccgccga ggtttgccag taacccggca acggttcgtg
    52921 ccagcaagat ccccaacagc agaccgctca taatggtgcc aaccactttg ccgcgtttat
    52981 ccggtgaagc cagcgtcgct gccagcggaa ccagaatttg tgccacgact gagaataaac
    53041 cggttaatgc cgtaccgagg atcatcatcg ccagcgactg actgctggca gtgattaaca
    53101 tgccgcctgc cgccagtaag gtcatcgaga caatcaggcg gcggcgttca aacatatcac
    53161 cgaggggaac aagaaacagc agacctgctg catagcccaa ctgcgcggcg gtaacaataa
    53221 agcctgccga actggcggaa agggaaaagt tacgcgcgat ggtgtcgagc aatggctggg
    53281 cgtaatagtt actggctacc gcctgaccgg tggcgataga catcagcacg atcagcgccg
    53341 ggctaagctc atgattaggt ttagtcatta tttttgttca tcaggtgatg attaagctgg
    53401 aaatcaataa tagcggatgt tgatgaatgg gggcgaaaga tagcttgttg aaatgtgcga
    53461 tgtggtttgt aggcatgata agacgcgcca gcgtcgcatc aggcattgtg cacagaacgg
    53521 cggatgcggc gaaaacgcct tatccgccat acggaatgag tgtattttgt aggcatgata
    53581 agacgcgcca gcgtcgcatc aggcattgtg cacagaatgg cggatgaggc aaaaacgact
    53641 tatccgcccg aataaaaatt acttctgcgc tgccagcgcc tcattcaccc agccatcgaa
    53701 ctgctgctgg tgggctttga tccaaccatc aacatggccc tgaatatcgc cttctgaggc
    53761 tttgccgtca tgcataatgg cgttctgggc gttaatatct gccactggca actgcataat
    53821 ggcaaaaagt ttcgctgctg ccgggttttt ctcggcccag gctttgttgg caacgatatg
    53881 catggtgctg accgggaagc cataattcgc accattcggc agtttggtat cggcgttttt
    53941 atcgcccggc agtgcggaga acggcacctg caaccagacc acatctttcc ctggcttcag
    54001 ctcattactc acccagtacg gcgtccaggt gtaataaaac accggtttgc cctctttgta
    54061 gcgactgatg gtgtcggcca tcatcgctgc gtagttcccc tgattatgcg tcacggtgtt
    54121 ggtcagttca tacgcggcaa gctggtggtt gatcgcacct tcgcagcccc agcctgggtt
    54181 acagccggtt aaatccgctt taccgtcgcc gttggtatcg aacagtttgg cgatcttcgg
    54241 atctttcagt tgtgcgatgt tggtgatttt gtactggtcg gcggttttct tatcgatcag
    54301 gtaaccctgt gccgcgccgt taacgaatat cccttcacaa taaaatttct tatcgccacc
    54361 ggcagcttcg tacatgttgt catgcagcgg cgtccagttc acggcggtga aggttgcatc
    54421 gccggaagca agtgaggtgt agccaacgtt gtaatccact tcgctgggtt tattgacggt
    54481 ataacctaat ttctccagtg cacgactgac cagcagcgtc tggaaggttt cttcagtgat
    54541 ggtgctctga actggattaa cagtaatgcc tttgcccggc agatcggctg caaaagtttg
    54601 tgtagagata agcgtggcaa acgctgtcgc aaaaagtacg ctatgtcgca ttgttattcc
    54661 tttttttggc agggtgataa tgcccggcgt taccgggcaa gtgcagagtt acttaatgaa
    54721 tgggcgggtc agcagaccga cagggccagt ggtgtaccag cgacggttgc cgcgactgcg
    54781 tgagtcgcgc ccaacggcct gcgtcaggcg gtcgaggata atggcgagga tcacaatccc
    54841 gacgccgcca acggtggcaa ggcccatatc cagacgaccg ataccgcgaa gtaccatctg
    54901 acccagcccg ccgacggcaa tcatcgaggc gatgaccacc atagaaagag ccagcatcag
    54961 cgtctggtta acgcccgcca taatggtcgg catcgccagc ggtaactgaa ctttgaacag
    55021 catctggcgc gggctggcac cgaatgagcg cgaggcttca atcagatccg ccggaacctg
    55081 gttaatcccc agaatcgtca gacggataat cggcggcaga gcaaagatga tcgtcaccac
    55141 tacgcccggc acgttaccga taccaaacag catgacgatt ggtaccagat aaacaaacgc
    55201 tggcgtggtc tgcatggcat caagcagtgg acgaataatt ttcgccgctc gcggacttct
    55261 cgccagccat atccccaacg gcaaaccgat gacgatacag aacagcaggg cggttaacac
    55321 cagcgccaga gtcaccattg cctgcgacca ggcaccgatt gcgccgatgg caatcagcga
    55381 aaccagcgtc gccacgccca ttccgacccc ggaaatctgc caggcgatga gagcgaaaac
    55441 gataatcgcc actggcgctg gcatacccag cagcaattgc tggaaactgt tgaggatata
    55501 atcaaccgga acgcgcacgc cctggaagac gggacggaaa tgggtaacaa cccagtcgat
    55561 cccttcagtg acccaactgt cgagcgggat cagcgtttta tggaacggat cgagaatatt
    55621 aaaatgctcg acgtttggcg caggcgtact ggtcagccag tcagcaccac cgccgtcagt
    55681 cggtgcagtc gtcggtgtac cccaggcgtc tgcggattgc gcggcactgt ccgccgctgg
    55741 cgtggtatcc cacggattat tttgatcagc cattatttac cccctcacga tctaaagcgc
    55801 gcagcagcat tcctttcgaa atgatgccga catactgttg gtcctcgtcg accacgggca
    55861 ccgcacaggg tgcctgtccg acatgagaga gcaactcgct aagaggcgtt tgtgcatcga
    55921 ctgctaacgg cgcatcaatc agcgccgcat caagaccttg ctgctgcgtt aacgcggttt
    55981 taagcgaatc gatggagact gcgccgacaa acttattacc gcgttcgata acgtagccat
    56041 attcgcgatc ttcatcctgc aataatttca gtgccgaacg tgggccgaag ccaggggttt
    56101 tacgaattaa gccattcggt gtccggcggg caatatcttt cgcactgaat acctgactaa
    56161 tatcaacgcc acggaagaag gtacggacat aatcattcgc cggattattg agaatttcat
    56221 ccggtgtgcc gacctgtacc acttcaccat tttgcataat ggcaattcgg tcgccaatac
    56281 gcatggcttc atcgagatcg tgggaaataa agacaatggt gcgctgatgt ttcgcctgta
    56341 attttaccag ctcatcctgc atctcggtgc gaattaatgg atcgagcgcc gagaaggctt
    56401 cgtccattaa taaaatatcc ggattaatcg ctaacgcgcg ggctaatccc acacgttgac
    56461 gcatcccgcc agagagttca tccgggtagc tgtgggcata attttccagc ccgacctgac
    56521 gcagtgcatc aagggctttt tcccggcgtt cttcggcatt aattccggcc aattccatac
    56581 cgaacgcagt attgtccagc acggtcatat gcggcattaa ggcaaaggac tggaagacca
    56641 tcgcaatctt ttttctgcgc acctcacgga gtttggcatc agatattttg gcaatatcca
    56701 caccatcaat cagcacttgc ccgcgggtgg gttcaatcag gcgattgaga aggcgtacca
    56761 ttgtggattt acccgagccg gataatccca tgatgacaaa tatctcgcct tcttcaatgg
    56821 ccagactggc gtcttttacg ccaagcgata gcccagtttt ttccagaatt tgttcttttg
    56881 aaagtccttg ttcgatatat ttgaatgctc gctgtggatg ctcaccaaat attttataaa
    56941 ggtttttaat ttctaattta attgccatgc aatagaaaga ttcctgttat ttgtttatgt
    57001 cgatatatta cccaatggaa atagttactt ttttctaccc taacatactg agaatctgag
    57061 gcaacccctg atggcaaatg tggcatgaat attgatgcga gcaaatcgcc ggaatttccc
    57121 gtgatataag ggctgagagc aaatcgagaa aagttgatat tttttgtcaa agatagcgaa
    57181 aaaacgcctg aatattgtgg tttcaggcga ataatgcaac agagtatcag tgtagatcac
    57241 cacaaaatat ttgcgtgata aaaagctatt tggcgggaat tatttccctg tcgcgggtag
    57301 tgatattttt gaaaataaca ccctcagaaa ttccagtctt catcttctgt ttcaaccgct
    57361 ttccccatca cataagagga gcctgaaccg gaaaaaaaat cgtgattttc atcggcattc
    57421 ggcgaaagcg cggcgaggat tgccggattc acttccgcca tttctgcggg aaataaaggt
    57481 tcgtagccca gattcatcaa agccttattg gcgttgtaac agagaaacgc tttcacatcg
    57541 tcagcccacg gggtttcggc gtacagctca tcggtgtatt gcagctcgtt gtcgtagagt
    57601 tccagcaaca aatcgaaggc gaaactcttc aactcttcac gttgtcccag agatatcttt
    57661 tccatgtttt tctgatattt atagcctatg tagtaaccgt ggactgcttc atcacggata
    57721 atcagacgga tcaggtctgc ggtattggtc agctttccgc ggctggaaaa atacatcggc
    57781 agccagaaac cggaatagaa caaaaaagat tcaagaaaca cactggcgat tttctttttc
    57841 agcggatcat caccgcgata atgttgctga ataatctgag cttttcgctg caacggtgcg
    57901 ttttcttcac tccaggcgta ggcggcatcg acatctttgg tctggcacag cgtcgagaaa
    57961 atcgaactgt aagagcgggc atgaaccgct tccataaagc tgatattcga taataccgct
    58021 tcttcatgag gcgtgagtgc atcgggcatc agcgaaggcg cgccgataac attttgcagc
    58081 gtgtcgagca gcgtcaggcc agtaaaaacg cgcatcgtca gttgttgttc tacgacagtt
    58141 aatgtctgcc aggcaggaat atcgttcgac agcggcacct tttctggtag ccagaaattg
    58201 ctggtcaggc gattccacac ctccagatct ttatcgtcag atatcttgtt ccagttgatg
    58261 gcgctgatac gtgagagttt catagatatt ccttaaagtg cacaggagac gcagccttca
    58321 atttcagtgc cttccagcgc catctgacgc aggcgaatgt aatagagcgt tttgatacct
    58381 ttgcgccagg cgtaaatctg cgctttgttg atatcgcgag tggtggcggt atcggggaaa
    58441 aacagcgtca gcgacaaacc ctgatcgaca tggcgagtcg cttccgcgta ggtgtcgatg
    58501 atcttttctg cgccgatttc gtaagcatcc tgatacagcg ccagattttc gttagtcata
    58561 aacggggcag ggtagtaaac gcgtcctgtt ttgccctctt tgcgtatctc tactttcgcc
    58621 acaatcggat gaatactcga cgtagcatgg ttgatataag agatagaacc ggttggcggc
    58681 accgcctgaa gattctggtt gtaaatgccg tagcgcatca cgtcgtcgcg cagttgcgcc
    58741 cacatctcac gggtaggtaa cgtaataccg ctgcgggcaa acagttcgcc aactttcgcc
    58801 gttttcggct gccagttacc ttgcagatat tggctaaaat attcaccact ggcataacgt
    58861 gactgtttga acccggcgaa ggtttcaccg cgttcgcgcg ccagcaacat cgaggtacgc
    58921 agtgcgtgcc aggtgatggc atagaaatag agattggtga aatccagtgc ttccggcgaa
    58981 ccataagcga tgccttctcg cgccagatag ccgtgtaaat tcatctgccc cagtccgatg
    59041 gcgtgtgagg cggcatttcc ggcttcgatg gacggcacgc tgcggatatg actcatatct
    59101 gacaccgccg ttaaaccgcg cacggcagtc tctaccgtgc gggcaaaatc gggggaatcc
    59161 atggtgtgcg caatattcaa cgaacctaaa ttgcaggaga tatcatggcc tgtgcgggca
    59221 tagtcgagat tctcgtcata ctctgaggca cagttgacct gcaaaatctc tgagcagaga
    59281 ttactcatat ttatgcgccc ggcgacaggg ttagcacggt ttaccgtgtc ttcatacatg
    59341 atgtagggat agccggactc aaactggatt tctgccagcc gctggaagaa atcacgggcg
    59401 ttgaggtatt ttttgcggat gcgttcatcg gcaaccagtt cgtcatagtg ttcgctgatg
    59461 gcgatatcgg caaacggctt gccataaact cgctcaacgt catagggcga aaacagcgcc
    59521 atctgcgcat tctcttttgc cagatggaaa gtgatatccg ggatcaccac gcccagcgac
    59581 agtgttttaa tgcggatttt ttcgtcggca ttttcccgtt ttgtatcgag aaaacgcaga
    59641 atatcgggat gatgagcatg taaatagact gcacctgccc cctgacgcgc gccgagttgg
    59701 ttggcatagg aaaatgcgtc ttccagcatt ttcatcaccg gaattacgcc agaagattga
    59761 ttttcaatac gtttaattgg cgcgcccgct tctcgcagat tcgacagcaa aaatgccacg
    59821 ccgccgccgc gtttcgacag ttgcagtgcg gaatttaccg cccggccaat cgactccata
    59881 ttgtcttcaa tacgcagcag aaaacaggaa accagttcgc cgcgctgctg cttaccgcag
    59941 ttgaggaatg tcggcgtggc tggctgaaag cgtcctgaca gcatttcatc ggtcagttgc
    60001 aacgccagcg tcgcatcgcc ttgtgccagc gtcagcgcca ccatcgtgac tcgatcggca
    60061 aaatcttcca gataacgttt accgtcgaat gtcttcaacg tatagctggt gtaaaacttc
    60121 catgccccga ggaatgtctg gaaacgaaaa ccgctggtgt gtgcgtgggc aaacagcgta
    60181 atgacaaagt cgcgagagta gcgattaaga acgctttcat catagtaacc ttcgttgacc
    60241 agccagttca ggcgctgctg ctggctactg aaggtcacac tgttcggacg cacatgcgtc
    60301 gccataaagg cgtcaacggc ctggcggtct ttatcgaact gaatgcgacc tgcgctatcg
    60361 tagaggttaa gcatcgcatt cagcgcgtgg taatccatcg tttcctgcgt caggcattct
    60421 gcggttgtcg ttgccaaaat tcggttactc ctttacgaac gttttcgata tcgctttgcg
    60481 tacccatgag ttcaaaacgg tacagccacg gcacgccgca tttccgggca atcacatctc
    60541 cggcgcggcc atacgcctca ccaaagttgc gattaccaga agcaataacg ccgcgaagca
    60601 acgcccggtt gtgctcgtcg tttaaaaagc gaattaccta tcgtggcacc gcgccagccg
    60661 taccgccgcc gccgtaagag ggcacgatca ggatgtaagg ctcgtctacc tgaatccgtt
    60721 cccgctcatt gagcgggatg cgcaccgcgg gcagacctaa acgttcgata aaacgctgcg
    60781 tgttttcgga gctgctggag aagtagacga gctggctcat gcactggccg cgtgtggcgc
    60841 tggatgcaga cggttaatca tgtccggacg gaaaccagac cagctaagat cgccagcaat
    60901 cactaccggc aactgacgaa agccctgagc acgcaacgct tccgctgctt caggaacgcg
    60961 atcgacatta atcatttcaa aatcaaagcc ccggttttcc atcgcccgtt tggtggcgtg
    61021 gcactgaacg caatcgttac gagtgtaaat agttatgcgc atgattcgta tttccgttta
    61081 aaatgaagat acggcgcgat gatacgcgtc gggttgtctc tctgttgata cagagatact
    61141 agatgtagtt gaaaaaagat tcaaccacac aatatatagc aaatagtgat cgaaattacc
    61201 ctagatatga gcgtgacggg ggagggggat ttttgtgatt caccaggcaa aaagaaaccc
    61261 cgaagacagg cttcggggtt aaagacgcgt atttattatc atttttgcac tacgatttgc
    61321 gcatgcttaa cagtgcgccg ataaaaatac ctaccgcagc tgctgtaccc acgctacacc
    61381 agggtctttc acgaacaaaa gaatccgcgc agccaacggc atcgcgtgcg gcttgctgga
    61441 cgcgagtacg accatgcatt cgcgccctgg tttctttcag caatgcctga gctttactgc
    61501 gtgcggcttc agcttcccct ttggcgtcgc tgccccagga cttcaataca gattcgaggc
    61561 tgtcagctaa ttgattgaca tcattctgga tatcctgcac gccatcatca acgtcgttgc
    61621 ggttcggtcg gttaaacata tgatccccca ttagaattcg atgtaatttt cagtgtagac
    61681 cataattttc ttaactgagg ttcatcgcgg ctttatggac ttttctgaaa gcgttgtgaa
    61741 tgcgcaatca ggtaaggtag gcccgcggtt gataataacg aggatcaaat atgtatttac
    61801 gaccagacga ggtggcgcgc gtacttgaaa aagtcggttt tactgtcgat gtggtaaccc
    61861 agaaagctta tggttaccgc cgtggggaaa attatgtcta tgttaatcgc gaagcgcgaa
    61921 tgggacgtac cgcactggta attcatccaa cattaaaaga acgtagttcg acactggctg
    61981 aacccgcttc cgatattaaa acttgcgatc attatcaaca atttccgctc tatttagcag
    62041 gcgagcgaca cgagcattac ggtatcccgc atggcttcag ttcgcgtgtt gcgcttgaac
    62101 gttatttgaa tggtttattt ggcgaagcca gttaaagacg cgactggcga tgccagtcgc
    62161 gaaaagaaga gtcaggcttt tacctgctgg taatggctga ctttaaacag tcggcggcaa
    62221 taatcgagga agtagccata aaccgccccc atcaacatcg aaacaacgat gtttgaactg
    62281 accgccgcca taatctgatg ccagtctgcg cccaccacta acaagatcgc cacatacacc
    62341 ggtgactgga acgtcacata agccaggata tcagccagat tttttatcca gcccgacggg
    62401 ctaactttgc gtgccgcgcg cataaataga tcgcggtaca taccgtatgg ccatgcaatt
    62461 aagatgttca ccggaatcgc taccaatctg gaataaaaag actgttcgaa gctcattccg
    62521 gagaggaaaa cttcaataca catgttcacg acagaacagt aaacaaccat cgcgaacgta
    62581 tctgcaactg catgacgcaa gcgtgactgc ggtgagaaca tgctaatgct ccttaataaa
    62641 agcgtaatgg gatgttaatg gagatgaaag cagtggatct cattgccatc tgcattttcg
    62701 catagcgtat atcgctggct atgactattc aattagtgaa aaatttttat ttagtcagtt
    62761 tttgtagata aaatattccg taacggctcg ttttttgtac attgtggatt tttacatgct
    62821 gatatgattt attttcaatt aaatcaatta gatatgacct tgcctttaat ttcactaatg
    62881 aaagtttggg ggtgaggtaa cgctataagc gtagagaatt cggtgtatgt cgtgaccgtg
    62941 tcatttacag catttttgat gccagaaaat gtacattaac agcagaaagc accagtgagg
    63001 gggg
//
LOCUS       DAJTVJ010000006        60881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000006 DAJTVJ010000000
VERSION     DAJTVJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.006"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..468
                     /locus_tag="OO448_000360"
     CDS             <1..468
                     /locus_tag="OO448_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6335676.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="OO448_000361"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="OO448_000361"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6335677.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="OO448_000362"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="OO448_000362"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6335678.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3349)
                     /locus_tag="OO448_000363"
                     /pseudo
     CDS             complement(2530..3349)
                     /locus_tag="OO448_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            3623..4003
                     /gene="panD"
                     /locus_tag="OO448_000364"
     CDS             3623..4003
                     /gene="panD"
                     /locus_tag="OO448_000364"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6335679.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4007..5236)
                     /locus_tag="OO448_000365"
     CDS             complement(4007..5236)
                     /locus_tag="OO448_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6335680.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5300..5740)
                     /locus_tag="OO448_000366"
     CDS             complement(5300..5740)
                     /locus_tag="OO448_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6335681.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5845..6615)
                     /locus_tag="OO448_000367"
     CDS             complement(5845..6615)
                     /locus_tag="OO448_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6335682.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6612..7538)
                     /locus_tag="OO448_000368"
     CDS             complement(6612..7538)
                     /locus_tag="OO448_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6335683.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7647..8309
                     /gene="can"
                     /locus_tag="OO448_000369"
     CDS             7647..8309
                     /gene="can"
                     /locus_tag="OO448_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6335684.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8350..8886)
                     /gene="hpt"
                     /locus_tag="OO448_000370"
     CDS             complement(8350..8886)
                     /gene="hpt"
                     /locus_tag="OO448_000370"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6335685.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9092..11482
                     /gene="gcd"
                     /locus_tag="OO448_000371"
     CDS             9092..11482
                     /gene="gcd"
                     /locus_tag="OO448_000371"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6335686.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VSAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11529..13079)
                     /gene="cueO"
                     /locus_tag="OO448_000372"
     CDS             complement(11529..13079)
                     /gene="cueO"
                     /locus_tag="OO448_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6335687.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13245..13592
                     /locus_tag="OO448_000373"
     CDS             13245..13592
                     /locus_tag="OO448_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6335688.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13698..14564
                     /gene="speE"
                     /locus_tag="OO448_000374"
     CDS             13698..14564
                     /gene="speE"
                     /locus_tag="OO448_000374"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6335689.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14580..15374
                     /gene="speD"
                     /locus_tag="OO448_000375"
     CDS             14580..15374
                     /gene="speD"
                     /locus_tag="OO448_000375"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6335690.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15412..15774)
                     /locus_tag="OO448_000376"
     CDS             complement(15412..15774)
                     /locus_tag="OO448_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6335691.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15949..18546)
                     /gene="acnB"
                     /locus_tag="OO448_000377"
     CDS             complement(15949..18546)
                     /gene="acnB"
                     /locus_tag="OO448_000377"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6335692.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18901..20658
                     /locus_tag="OO448_000378"
     CDS             18901..20658
                     /locus_tag="OO448_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6335693.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20729..22153)
                     /gene="lpdA"
                     /locus_tag="OO448_000379"
     CDS             complement(20729..22153)
                     /gene="lpdA"
                     /locus_tag="OO448_000379"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6335694.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22478..24370)
                     /gene="aceF"
                     /locus_tag="OO448_000380"
     CDS             complement(22478..24370)
                     /gene="aceF"
                     /locus_tag="OO448_000380"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6335695.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24385..27048)
                     /gene="aceE"
                     /locus_tag="OO448_000381"
     CDS             complement(24385..27048)
                     /gene="aceE"
                     /locus_tag="OO448_000381"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6335696.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27209..27973)
                     /gene="pdhR"
                     /locus_tag="OO448_000382"
     CDS             complement(27209..27973)
                     /gene="pdhR"
                     /locus_tag="OO448_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6335697.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28514..29887
                     /gene="aroP"
                     /locus_tag="OO448_000383"
     CDS             28514..29887
                     /gene="aroP"
                     /locus_tag="OO448_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6335698.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29930..30784)
                     /gene="ampE"
                     /locus_tag="OO448_000384"
     CDS             complement(29930..30784)
                     /gene="ampE"
                     /locus_tag="OO448_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6335699.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30781..31332)
                     /gene="ampD"
                     /locus_tag="OO448_000385"
     CDS             complement(30781..31332)
                     /gene="ampD"
                     /locus_tag="OO448_000385"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6335700.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31420..32313
                     /gene="nadC"
                     /locus_tag="OO448_000386"
     CDS             31420..32313
                     /gene="nadC"
                     /locus_tag="OO448_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6335701.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32516..32956
                     /gene="ppdD"
                     /locus_tag="OO448_000387"
     CDS             32516..32956
                     /gene="ppdD"
                     /locus_tag="OO448_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6335702.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32966..34352
                     /gene="gspE"
                     /locus_tag="OO448_000388"
                     /pseudo
     CDS             32966..34352
                     /gene="gspE"
                     /locus_tag="OO448_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34342..35544
                     /gene="hofC"
                     /locus_tag="OO448_000389"
                     /pseudo
     CDS             34342..35544
                     /gene="hofC"
                     /locus_tag="OO448_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35579..36622)
                     /gene="guaC"
                     /locus_tag="OO448_000390"
     CDS             complement(35579..36622)
                     /gene="guaC"
                     /locus_tag="OO448_000390"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6335703.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36778..36822)
                     /locus_tag="OO448_000391"
     CDS             complement(36778..36822)
                     /locus_tag="OO448_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335704.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36847..37467
                     /gene="coaE"
                     /locus_tag="OO448_000392"
     CDS             36847..37467
                     /gene="coaE"
                     /locus_tag="OO448_000392"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6335705.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37467..38210
                     /gene="zapD"
                     /locus_tag="OO448_000393"
     CDS             37467..38210
                     /gene="zapD"
                     /locus_tag="OO448_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6335706.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38220..38417
                     /gene="yacG"
                     /locus_tag="OO448_000394"
     CDS             38220..38417
                     /gene="yacG"
                     /locus_tag="OO448_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6335707.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38517..38906)
                     /gene="mutT"
                     /locus_tag="OO448_000395"
     CDS             complement(38517..38906)
                     /gene="mutT"
                     /locus_tag="OO448_000395"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6335708.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38966..41671)
                     /gene="secA"
                     /locus_tag="OO448_000396"
     CDS             complement(38966..41671)
                     /gene="secA"
                     /locus_tag="OO448_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6335709.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41733..42245)
                     /gene="secM"
                     /locus_tag="OO448_000397"
     CDS             complement(41733..42245)
                     /gene="secM"
                     /locus_tag="OO448_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6335710.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42476..43393)
                     /gene="lpxC"
                     /locus_tag="OO448_000398"
     CDS             complement(42476..43393)
                     /gene="lpxC"
                     /locus_tag="OO448_000398"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6335711.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43494..44645)
                     /gene="ftsZ"
                     /locus_tag="OO448_000399"
     CDS             complement(43494..44645)
                     /gene="ftsZ"
                     /locus_tag="OO448_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6335712.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44706..45968)
                     /gene="ftsA"
                     /locus_tag="OO448_000400"
     CDS             complement(44706..45968)
                     /gene="ftsA"
                     /locus_tag="OO448_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6335713.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45965..46795)
                     /gene="ftsQ"
                     /locus_tag="OO448_000401"
     CDS             complement(45965..46795)
                     /gene="ftsQ"
                     /locus_tag="OO448_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6335714.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46797..47717)
                     /gene="ddlB"
                     /locus_tag="OO448_000402"
     CDS             complement(46797..47717)
                     /gene="ddlB"
                     /locus_tag="OO448_000402"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6335715.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47710..49185)
                     /gene="murC"
                     /locus_tag="OO448_000403"
     CDS             complement(47710..49185)
                     /gene="murC"
                     /locus_tag="OO448_000403"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6335716.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49239..50306)
                     /gene="murG"
                     /locus_tag="OO448_000404"
     CDS             complement(49239..50306)
                     /gene="murG"
                     /locus_tag="OO448_000404"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6335717.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50303..51547)
                     /gene="ftsW"
                     /locus_tag="OO448_000405"
     CDS             complement(50303..51547)
                     /gene="ftsW"
                     /locus_tag="OO448_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6335718.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51547..52863)
                     /gene="murD"
                     /locus_tag="OO448_000406"
     CDS             complement(51547..52863)
                     /gene="murD"
                     /locus_tag="OO448_000406"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6335719.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52866..53948)
                     /gene="mraY"
                     /locus_tag="OO448_000407"
     CDS             complement(52866..53948)
                     /gene="mraY"
                     /locus_tag="OO448_000407"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6335720.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53942..55300)
                     /gene="murF"
                     /locus_tag="OO448_000408"
     CDS             complement(53942..55300)
                     /gene="murF"
                     /locus_tag="OO448_000408"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6335721.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55297..56790)
                     /gene="murE"
                     /locus_tag="OO448_000409"
     CDS             complement(55297..56790)
                     /gene="murE"
                     /locus_tag="OO448_000409"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6335722.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56777..58543)
                     /gene="ftsI"
                     /locus_tag="OO448_000410"
     CDS             complement(56777..58543)
                     /gene="ftsI"
                     /locus_tag="OO448_000410"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6335723.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58559..58924)
                     /gene="ftsL"
                     /locus_tag="OO448_000411"
     CDS             complement(58559..58924)
                     /gene="ftsL"
                     /locus_tag="OO448_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6335724.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58921..59862)
                     /gene="rsmH"
                     /locus_tag="OO448_000412"
     CDS             complement(58921..59862)
                     /gene="rsmH"
                     /locus_tag="OO448_000412"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6335725.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59864..60322)
                     /gene="mraZ"
                     /locus_tag="OO448_000413"
     CDS             complement(59864..60322)
                     /gene="mraZ"
                     /locus_tag="OO448_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6335726.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14583 a        16821 c        14990 g        14487 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ccgtttccct gtctctcagc acaccataaa acaaatccgc ttgttcagta tgaccgcgtt
     2701 gcagcatata gttttgcatg gcaactaact gacttccgct agtgtaccct tcgtcaatca
     2761 gcgtgaccag ttgctccagc aataacatta agtcgcgctg gcgaatatgt ttttgcagta
     2821 gttcgagaat cgcaatccgc cgatgttgca tgatttcgtc atccggtgtg atggtgatat
     2881 ccaccagcgg gaaaggactg ttatagacgc gtcgtgccag ctccggcgag taaaatatat
     2941 caaaccagca cattgagagc ggataaggtg tggcctcgcc ctgataaaac agtatcggca
     3001 ccaccagcgg cagcttatcg tgatccgcct ccagatgccg gtgcatggca gcaatagaat
     3061 aacgcatcat gcgaaagacc attttcttat ccggcttgct ttggtgttca atcacaacat
     3121 gcagataacc gggattgccc tgcatttgca cggaatagag cacgtccgtg ctgtgtcctt
     3181 tcaggctctc ttcaatgaaa ctccccgact ctaaatgaag cgtgttgagg tcacaaagtt
     3241 ctcgtaattc cactggcaaa tgtatctcca gaaagtcgcg agccgtctcc gcgtgcatta
     3301 aaaattgttt aaacactgca tcatgcggtg tggtactcgg tgcatccatc ttccctgcct
     3361 tccgtgttgt cttgcgtgaa ggcagtatcc agtcaaggcg tgccatcgtc agccacctct
     3421 ttagtgttta ccgagcagcg ttcagagaaa tttcaattgg ttacagccgt tacatggcac
     3481 gcttcggcac ggtacgcaaa ctgctgatgt aaatgccgtc agatgagtag acactaaaca
     3541 aaaatcgggc aatactgcgt gagaatttcc tcaaagcagg catcgcctgc ttcgttaacg
     3601 acagggtaga aaggtagaag ttatgattcg cacgatgctg cagggcaaac tccaccgcgt
     3661 gaaagtgact catgcggacc tgcactatga aggttcttgc gccattgacc aggattttct
     3721 tgacgcagcc ggtattctcg aaaacgaagc cattgatatc tggaatgtca ccaacggcaa
     3781 gcgtttctcc acttatgcca tcgcggcaga acgcggttcg agaattattt ctgttaacgg
     3841 tgcggcggcc cactgcgcca gtgtcggcga tattgtcatc atcgccagct tcgttaccat
     3901 gccagatgaa gaagctcgca cctggcgacc caacgtcgcc tattttgaag gcgacaatga
     3961 aatgaaacgt accgcgaaag cgattccggt acaggttgct tgattgttat ccctgcggct
     4021 ggttactcac cagccgcgac atcgtctcca gcgaatccgt tcttaagata taaagtcgtt
     4081 ttagtaacaa cggattatcc cccggtttta ctttgccttt catggttgtc accgccaggt
     4141 gaaatccggc atcgtttgct gccttcacgg ctttgtcatt aaatccgcca aacggatacg
     4201 aaagatacca gacatgcgga ttaaattgcg ccagagcgcg gcgtgaacgt gcaaaatcaa
     4261 acagaatatt gtgctcacta cggctcagta atatgggtcg gcgatagcca tccacccgat
     4321 gcaaaaaatg ggtatgtgac tgaaaatcaa aaacatcgcg aatttcgtta agctcagaaa
     4381 cgctcataaa ttgcagcgat tttgggttcc acttctgcgg gtgacgtttg atgcgcgagg
     4441 taacaacaaa cgccgtcgcc ttcatgccat attgtttcaa tacaggatac gcatagcggc
     4501 tcaccgactt gaggccatca tcaaaggtaa tcaccaccgc tcgcgcaggg agattgatct
     4561 tattcttcac gtaaccttcc agctgcgcca tgctcagtgt cgcgtatccc ctgtcgcgca
     4621 gccaggccat ttggttattg aaagcgcgta ccgaggtggt cgtcgaagta tggcgaaaac
     4681 gggtgttttc ttcgtcgcgc agaatatggt gataagttag caccggaagg ccattatcgg
     4741 gttgggcatc cagcgcgctg atataggcca gtcgatcgcc aatacggacc tgataccagg
     4801 tttgatttaa cctgtctttc agtttatgca aaatcgggta gcgcaaattg tccgccaata
     4861 ccccaaatgg cgcactgccc acactcggcg cgttatagac cggcgtatct ttccaggtaa
     4921 ctaagttctg attactcagc ggcttgttga ggtcacccaa accgtcttca actttttgtc
     4981 gcccctgaac cggctcgaga tgccctttat cgataaaccc tttaccaaag ccaaaattaa
     5041 atgcgtaata acttgcggca gtgggctcca cggcaatgat ttgtccggcc cgaatattcc
     5101 ccacggtcac catcttgtcg ccaatttgcg cccagaccgc agcattttcg atggtttgca
     5161 tataacgggc aggtaacgcg gcactgacac ttgcggtaaa cagcatcagc aggagaataa
     5221 cagcttgttt gtacatgaaa aaataaccga gggtgaaaca acgcttttat tgtagcaaaa
     5281 gcgctataag aatcgtgggt taatactcat acaaattatg tttgaggacg aaaggcggat
     5341 ttttttgttg ttggtgccaa agactactca cctccggccc acccagttcg acaatacgct
     5401 cgcgaaactc tgaactggtc atggtttcac ggcaagccat catctgttca attaacggta
     5461 acgtgacacc agaaatcact tcgcaacggg agtgtttgtg gcttaataat gaagccacgc
     5521 ggtacggtgg cgctccggct atatcggtta agaagatgac accctcacca gagtccgctt
     5581 catgcagagc atcgcacatc atgcggctga gcatattaga gcttaaccca cgccagaaat
     5641 tcacggcccg gcactgaaga agcgccccat gttttttctc cagcgcatcc agtatctctt
     5701 gcgcccggtc atcgtgacag gtaattaccc aacctaacat cgccttcctc cttagccgaa
     5761 aatcagtcta ccctgaccat tgttgataaa gggtgatagc cgtcaaaact cctctccccc
     5821 aaatccagag gagaggaaat agccttagct acgcaaacca cgtccacgtt ggatcagcga
     5881 ccaacagatc aaataaaacg ccacaataaa gaccaccagt acgccaaagg tagtgaccag
     5941 cggaacatca ttgataccga ggaagccgta gcggaaacca ctgatcatat aaacgattgg
     6001 gttcaggtgc gacagccctt gccagaacgg cggcaacaaa gtcagtgagt aaaagacccc
     6061 acccaaatac gtgagtggcg ttaacacaaa ggttggcacc aggctgatgt catcgaacgt
     6121 tttggcaaac acaccgttca gcaaacccgc aagggagaac aacaccgccg tgagcaccag
     6181 ggttaaggca acgaataccc acgaatgcac ctgaaacggc acaaaaaaca gtgaaattgc
     6241 cgtcaccaga atgccaacaa acagaccacg cgccacgcca ccgccgacat atccggcaat
     6301 aatgacgtga gtcggaaccg gcgctaccag cagctcttca atattacgct ggaacttggc
     6361 accaaaaaat gatgacgcaa cgttggcgta ggcattggtg atcaccgaca tcatgatcag
     6421 ccccggtacg atgaactgca tatagctgaa gccatgcata tcgccaatac gcgaaccaat
     6481 caggttaccg aagataataa agtaaagggt catggtgatg actggcggca ccagcgtctg
     6541 cacccagata cgcataaagc gatggatctc tttcgcccag atgcttttta gcgccaccca
     6601 gtaaagatgc atcatgcgcg atctccttgt ttttcattaa ccagtgaaac aaacagctct
     6661 tccagacggt tagctttgtt acgcatactt aatacctgaa tgccctgctc acttaactgc
     6721 gtaaatacgc tgttgatccc ctgctcacgc agcacttcaa cttccagcgt cgcggtatcg
     6781 accagtcgat actgatagcc atcgagcttc ggtaacggac ttttcggtgc gagatcgaga
     6841 ataaaggttt ccgatttcag cttcgccagc agcgccttca tcgaggtatt ttccaccagc
     6901 tcaccgtgtt gaataatgcc gatattgcgg cacagcattt ctgcttcttc caggtagtgt
     6961 gtggtgagaa tgatggtggt gcctttgtcg tttaaatcct tcaaaaagcc ccacattgag
     7021 cggcgaagtt caatatccac gcctgcggtc ggttcgtcga gaatcagtag tttaggttca
     7081 tgcattaacg cacgggcaat cattaaacgg cgcttcatcc cgccagataa catacgcgca
     7141 cgttcgttgc gttttcccca tagatcgagt tgtttaagat atttttcgct gcggatgtac
     7201 gcctctttgc gctccacacc gtagtaccct gcctgattca ccacaatttg ctgcacggtt
     7261 tcaaacgggt tgaagttaaa ttcctgcggc accagtccca actgacgttt agcgttcacg
     7321 acatctttct cgagatcgta accaaatacg ctgacccgcc cggaggtttt atttaccaga
     7381 gagctgataa taccgatagt ggtcgatttc ccggccccgt tcggcccgag aagcgcataa
     7441 aaatcacccg cttcgacctg caaatctatt ccacgaagcg cctgaacgcc gcctggatag
     7501 gtttttttaa gctgttgaag ttccagtgca atggtcataa atttttactt accttacgtt
     7561 cttacacttt atctgtggtt taaatcgtcc cggagttgcc ctatattagc caaacgtaat
     7621 tatttggtta caggtcgtta acctccatga aagacataga tacactcatc agcaacaatg
     7681 cactatggtc aaaaatgctg gtggaagagg atcccgggtt ttttgagaaa ctggcacaag
     7741 cgcaaaaacc gcgctttcta tggattggat gttccgacag tcgcgttcct gcagaacgtt
     7801 taaccggtct tgagccgggc gaactctttg ttcaccgtaa tgttgctaac ctggtcattc
     7861 acaccgacct gaactgcctt tccgtggttc agtatgcagt ggatgtactc gaagttgaac
     7921 acattattat ctgtggccac tacggttgcg gcggcgtaca agccgccgtt gaaaacccgg
     7981 aactggggct tatcaacaac tggctgctgc atatccgcga tatctggttc aaacatagct
     8041 cattgctcgg cgaaatgccg caagagcgcc gtctggatac cttgtgtgaa ctgaacgtca
     8101 tggaacaggt ttataaccta ggccactcca ccattatgca atcagcgtgg aaacgcgggc
     8161 agaaagttac cattcacggc tgggcctacg gcattcacga cggcttgctg cgtgatctgg
     8221 atgtcacagc gaccaaccgc gaaacccttg agcaacgtta ccgtcacggg atttccaacc
     8281 tcaagctgaa acacgccaac cacaaataaa aatgccatgc cggatgcaac acatccggca
     8341 acttcacact tactcgtccc gcagaatcac tttgccgata tacggcagat gacggtaacg
     8401 ctgtgcgtaa tcaatgccgt aacccaccac aaactcatcc gggatcgaga aaccgataaa
     8461 ttctaccggg acgttcactt cacgacggga cggtttatcc agcagcgtac aaatcgccag
     8521 cgacttcggt tcgcgcaggc ttaagatctc acgcactttc gacagtgtat tccccgagtc
     8581 gatgatatct tcaacaatca gcacgtcctt gccacggata tcttcatcca gatctttgag
     8641 gattttcaca tcacgggtgg tggacatgcc gctaccgtag ctggaggcgg tcataaagtc
     8701 gacttcatga gatacctgaa cttcacggca caggtccgcc ataaacataa atgagccacg
     8761 cagcagaccc accagcacca tatcgctgcc gctgtctttg taacgctcag taatctgacg
     8821 acccagttcg gcgatacgcg ctttaatctc cgcttcgggg atcattactt ctacagtatg
     8881 tttcatatct ctaaccatat gatttaaaaa caaatcactt cgcacgggca ggttttggca
     8941 ccgcaacaaa gcgcaagaca cgcagtatac cagtaaaact attcattcgc ggaatctgtt
     9001 aataaagctc gaattgtgat gacggtcaca catgttaaaa cctataatta attgctacta
     9061 aaatattaat gaattgaaat ggtgtctctt tatggcaatt aacaatacag gctcgcgacg
     9121 attactcgtc acgctaacag ccctttttgc agcgctttgc gggctgtatc tactcattgg
     9181 cggaggctgg ctggtcgcga ttggcggctc ctggtactac cctatcgctg gccttgtgat
     9241 gctcggcgtc gcctggatgc tgtggtgcag taaacgcgcc gcgctttggc tatacgcagc
     9301 cctgctgctc ggcaccatga tttggggcgt ctgggaagtt ggtttcgact tctgggcgct
     9361 gactccgcgc agcgatattc tggtcttctt cggcatctgg ctgatcctgc cgtttgtctg
     9421 gcgtcgcctg gtcattcctg ccagcggcgc agttgccgca ctggtggtgg cactgctgat
     9481 tagcggtagt atcctgacct gggccggatt taacgatccg caggaaatca acggcacctt
     9541 aagcgccgat gccacacctg cggaagctat ctcacccgtt gccgatcagg actggcctgc
     9601 ctatggtcgc aatcaggaag gtcaacgctt ttcacctctg aaacaaatta acgccgataa
     9661 cgtccataat ctgaaagaag cctgggtgtt ccgtactggc gatgtgaagc agccgaacga
     9721 tccgggcgaa atcaccaatg aagtgacgcc gattaaagtg ggcgacaccc tttacctgtg
     9781 taccgctcac cagcgcctgt ttgcgctcga tgccgccagc ggcaaagaga aatggcatta
     9841 cgatcctgag ctgaaaacta acgagtcttt ccagcacgta acctgccgtg gcgtctctta
     9901 tcatgaagcc aaagcagaaa cggcttcgcc ggaagtgatg gcggattgcc cgcgtcgtat
     9961 cattcttccg gtcaatgatg gtcgactgat tgcgattaac gctgaaaacg gcaaactgtg
    10021 cgaaaccttc gccaataaag gcgtgctcaa tctgcaaagc aatatgccag acaccaaacc
    10081 gggtctgtat gaaccgactt cgccaccgat tatcaccgat aaaaccatcg tgatggccgg
    10141 ttcagttacc gataacttct caacccgcga aacgtctggc gtgatccgtg gttttgatgt
    10201 caacaccggg gagctgctgt gggcttttga tcccggcgcg aaagatccga acgcaatccc
    10261 gtctgacgaa cacaccttta cctttaactc gccaaactcc tgggcaccag cggcctatga
    10321 cgcgaagctg gatctggtct atctgccgat gggcgtgacc acgccggata tctggggcgg
    10381 taaccgcaca ccggaacagg aacgttatgc cagctcgatt ctggcgctga atgccactac
    10441 cgggaaactg gcgtggagct accagaccgt tcaccacgac ctgtgggaca tggatcttcc
    10501 ggcacagccg acgctggcgg acatcaccgt taatggtcag aaagtgccag ttatttacgc
    10561 tccggcgaaa accggcaaca tttttgtgct cgatcgtcgc aatggcgaac tggtggtttc
    10621 ggccccggaa aaaccggtgc cgcaaggtgc tgcgaaaggc gattacgtta ctccgaccca
    10681 gccgttctct gaactgagct tccgtccgaa gaaagatttg agcggtgcgg atatgtgggg
    10741 agccaccatg tttgaccaac tggtgtgccg cgtgatgttc caccagatgc gctatgaagg
    10801 cattttcacg ccgccatctg aacagggcac gctggtcttc ccgggtaacc tgggaatgtt
    10861 tgaatggggc gggatttccg ttgatccaaa tcgtgaagtg gcaattgcca acccaatggc
    10921 actgccgttt gtttcgaaac tgatcccacg cggtccgggt aacccgatgg agcagccgaa
    10981 agatgccaaa ggcacgggta cggaatccgg cattcagcca cagtacggtg taccgtatgg
    11041 tgtcacgctc aacccgttcc tctcaccgtt tggtctgccg tgtaaacagc cagcatgggg
    11101 ttatatctcg gcgctggatc tgaaaatcaa tgaagtggtg tggaagaaac gtattggtac
    11161 gccgcaagac agtatgccgt tcccgatgcc tgtaccggtg ccgttcaata tgggtatgcc
    11221 gatgctgggc gggccaatct ccacggcggg taacgtgctg tttatcgctg ctacggcaga
    11281 taactacctg cgcgcttaca acatgagcaa cggtgaaaaa ctgtggcagg gccgtttacc
    11341 agcgggtggt caggcgacgc caatgaccta tgaagtgaat ggcaagcagt atgtggtgat
    11401 ctccgcaggc ggtcacggtt catttggtac gaagatgggc gactatattg tggcttatgc
    11461 cctgccggat gatgtgaaat aaccataatg cccggagaga tccgggcata tttccgaata
    11521 cggtcttttt ataccgtaaa ccctaacatc atccccgtat cttcatgctc cagcagatgg
    11581 cagtgcgcca tataagcatg ttctttcggt gcgttgtgat taaacttcac cagcacttca
    11641 ctgacattgc cttctacctt aacggtatct ttccagcccg cgcgatgagc cgctggcggt
    11701 ttgccatttt ctgacaagat acggaactgc atgccgtgaa tatggaacgg atgcagcatc
    11761 atgtcgccca cgccagagat aacccaacgt tcgtactgcc ctttcgccgc cgcaaacatc
    11821 ggcttattca tatcaaacgc ctgaccgttg attttattgg catggtggaa atcgaacttc
    11881 ccgccgtggt tcatatgatt catattgccg tgccccatat ggcccatcat ctggctgtga
    11941 tccattccgg ccatcgcctg atcgccatat ttctccatta gcatctgcat ccccatcata
    12001 tcgagcatcg ggtccataga aagttgcagc ttgcgtaccg tcagcccttc cagcgaaggt
    12061 aacgcaggca ggctacttaa tgtgtcaggc aaagcaccgg aggcactaat agcaatcggc
    12121 tgaatccgca ttaccggatg cggcttgtca aacggcgcaa tcgccatccc catctggctg
    12181 accggtagcg tcaccagatc aaagggtttg ttgtcgttaa cctccaccag cacttcaaaa
    12241 cgctcgccca tcagcaccgg cagctcgctc accttcaccg gttcaggtag cagaccaccg
    12301 tcgctggcaa tcacatacag cgggcgattg tcgctggtgg cgaaattgag cgagcgggcg
    12361 ttacagccat tgagcaaacg caggcgcagc cagccacgcg gggcagcgtg ttgtgggtag
    12421 attgcaccgt tggtcagcag cgtatcgcca aaccagccca cggcggcggt catcacatcc
    12481 agttgataat caatctgccc atcagcgtta aattttttat cctgaacaat caccggaaca
    12541 tcatcgatac cccactgttt tggcagcatt aatttcagga tctcgtcatc ttcaattacc
    12601 accagcccag ccaaccccat cgccacctgt cgcccggtct tgccatgttg atgtggatgg
    12661 aaccagcagg tagcggcagg ttgatcaacg ttcaacgtca ccgtacgttt gccgcctggc
    12721 ggaataattc cctgcgggcc gccgtcgacg tcacccggta cttccagccc gtgccagtgc
    12781 aacgtcgtct cttccgccag ttggttatag atatcaaccg ttaccgcttt gccgcgctgt
    12841 aatttcaccg ccggccccag cagattgccg ttatagcccc aggtagttgc agttttctcg
    12901 ccaaaggtag actgacctgc gccaatagtt aactgaatgc gattacgggc atcggtcgtg
    12961 agcaaatcag ggatcggtaa cgttgggcgt tccgccgcaa agactgcgcg gctccacagc
    13021 ggcaaggctg aagccacacc cagcgcgacg gaatatttta agaaatcacg acgttgcata
    13081 gttatttcct tattcttaag caggcgaaac aaaatcaaac gttgagcata gtccttcccc
    13141 ttacgggaag gtcaagccgc agacacaatt taatcgacaa taatggtcgt cgcctcgctc
    13201 gcagtgtgct aacgtttatc ttctttaagc ccctggtaga agcaatgaag acgtttttca
    13261 gaacagtgtt attcggcagc ctgatggccg tctgcgcaaa cagttacgcg ctcagcgagt
    13321 ctgaagccga agatatggcc gatttaacgg cagtttttgt ctttctgaag aacgattgtg
    13381 gttaccagaa cttacctaac gggcaaattc gtcgcgcact ggtctttttc gcccagcaaa
    13441 accagtggga tctcagtaat tacgacacct tcgacatgaa agccctcggt gaagacagct
    13501 accgcgatct cagcggcatt ggcattcccg tcgctaaaaa gtgcaaagcc ctggcccgcg
    13561 attccttaag cctgcttgcc tacgtcaaat aatccctgat acctttttgt agaaatattg
    13621 accgtgcatc cgcggtcaat gttagctatt atgttgcgcc ctttttttac gggtgttaac
    13681 aaaggaggta tcaacccatg gccgaaaaaa aacagtggca tgaaacgcta cacgaccagt
    13741 ttgggcagta ctttgcggta gataacgttc tgtatcatga aaagaccgat caccaagatc
    13801 tgatcatttt tgagaacgct gcatttggtc gcgtaatggc gctggatggc gtagtacaaa
    13861 ccaccgagcg cgacgagttt atctatcatg agatgatgac ccatgttccg ctactggccc
    13921 acggtcacgc gaaacatgtg ctgattatcg gcggcggcga cggtgccatg ctgcgtgaag
    13981 taacccgaca taaaaacgtt gagtcaatca cgatggtgga aatcgatgcg ggtatcgtgt
    14041 cgttctgccg tcagtatctg cccaaccata acgccggtag ctacgacgat ccgcgcttta
    14101 agctggtgat cgacgatggc gtcaatttcg ttaatcaaac cagccagacc tttgatgtca
    14161 ttatctccga ctgcaccgat cctatcggtc ccggcgaaag tctgttcacc tcagcatttt
    14221 atgaaggctg caaacgttgc ctgaatcctg gcggtatctt cgtcgcacaa aacggcgtct
    14281 gctttttaca gcaggaagaa gccatcgaca gctatcgcaa actcagccat tacttcagcg
    14341 acgttggctt ttatcaggcg gcgatcccga cctattacgg cggtatcatg acttttgcat
    14401 gggcgacaga taacgacgcc ttacgccatc tctcaaccga aattattcag gcgcgttttc
    14461 tcgcttctgg cctgaaatgc cgttattaca atccggcaat ccatacggca gcttttgcct
    14521 tacctcagta tctgcaagac gcactggctt cacagccgtc ctaaggagaa gataagaaat
    14581 tgaaaaaact gaaactgcat ggctttaata atctgaccaa aagtctgagt ttttgtattt
    14641 acgatatctg ctacgccaaa actgccgaag agcgcgacgg ttatattgct tatatcgatg
    14701 aactctataa tgccaaccgt ctgaccgaaa tcctgtcaga aacctgttcc attatcgggg
    14761 ccaatattct taacatcgcc cgccaggatt acgaaccaca gggtgccagc gtcactattc
    14821 tggtgagtga agaaccggtt gacccgaaac tcatcgacaa aacagaacac cccggcccac
    14881 tgccagaaac ggtcgttgcc catctcgata aaagtcatat ttgcgtacat acctacccgg
    14941 aaagtcatcc tgaaggcggt ttatgtacct tccgcgccga tattgaagtc tctacctgcg
    15001 gcgtgatttc tccgctgaag gcgctgaatt acctgatcca ccagcttgag tccgatatcg
    15061 taaccattga ttatcgcgtg cgcggtttta cccgcgacat taacggtatg aagcacttta
    15121 tcgaccatga gattaattcg attcagaact ttatgtctga cgatatgaag gcgctgtatg
    15181 acatggtgga tgtgaacgtc tatcaggaaa atatcttcca taccaagatg ttgcttaaag
    15241 agttcgacct taagcactac atgttccaca ccaaaccgga agatttaacc gacagcgagc
    15301 gccaggaaat taccgctgcg ctgtggaaag aaatgcgcga gatttattac gggcgcaata
    15361 tgccagctgt ttaacggctc tggcggagct cccaggctcc gccagattta tttacttctg
    15421 ctgcacgaaa ttgcggtaag ccgccacgac ctgcagaaaa tcctcaacgc cgcatagcga
    15481 caggctttct tcgtcgtagt agttcatccc ttcttccatt tcatcgccag cgaattccag
    15541 ttgattggcg cgaaccatca cctcttcacc gtccatccac agggtgtatt catgccctgc
    15601 ccgttgccag gaccgttcgc tacctttcag tgcgtgcgct gcttgttcca cttcatcaag
    15661 caaagccagg ttttctttca cctcttcatt aaaccagtgc ccgaccactt catgccccat
    15721 ggacatacgc acctttacca ctccggtaat atcgcgcaga aattcgtaat ccatagtgtg
    15781 ttcctctgct cccggcaatg cgccgaaatc gcattgcgct attgatgtaa ttattgcagc
    15841 cttaccggag aaaaacagcg gaacggcgat ggtttagaaa taaaaaatgc ccgggaatct
    15901 cattcaccgg gcattgtgtc gtttatgcgc agcgcgtgcg cttacttatt acaccgcagt
    15961 ctggaaaatc accccatcgg ctttttcggt gtactgagaa agctggttga agttcagata
    16021 acgataagta tcaacggcag ttttatctac ttgcgcaacg taggtctggt actcttccgg
    16081 cgtcggcagt ttgccaatca gcgccgcaac agccgccagt tctgcagaag ccaggaagac
    16141 attcgcgcca gtccccagac ggttcgggaa gttacgggta gaggtggaaa ccaccgtcgc
    16201 accgtctgct acacgcgcct ggttacccat acacagagaa cagcccggga tctcgatacg
    16261 agccccgctc ttaccgaaga cgctgtagta gccttcttca gtcaactgtg cggcgtccat
    16321 gcgggttggc ggtgccaccc acaggcgggt cggcaactgg cctttgtgcg catccagcag
    16381 tttacccgca gcacggaagt gaccgatgtt agtcatgcaa gaaccgataa acacttcgtc
    16441 gatcttctcg ccctgtaccg cagacagcgg acgtgcgtcg tccgggtcgt tcggtgcaca
    16501 gaggattggc tctttaatat ccgccagatc gatgtcgatc actgccgcgt attcagcatc
    16561 tgcatcggct tccagcagct caggattcgc cagccatttt tccatgccct gaatacgacg
    16621 ttcgagggta cgacgatcgc cataaccttc cgcgatcatc cacttcagca ggacgatgtt
    16681 agagttcagg tattcgatga tcggttcttt gttcagcttg atagtacaac cagcggcaga
    16741 acgctcagcg gacgcatcgg tcagttcaaa tgcctgctca actttcagat ccggcagacc
    16801 ttcaatttcc agaatgcgac cagagaagat gtttttcttg cctttcttct caacggtcag
    16861 cagaccttgt ttgatcgcat acagcgggat cgcgtgtacc agatcgcgca gggtgatgcc
    16921 cggctgcatt ttgcctttga agcgcaccag aacggattcc ggcatatcca gcggcattac
    16981 gcctgttgcg gctgcaaatg ccaccagacc agaacccgcc gggaaagaga taccgatcgg
    17041 gaaacgggta tgggagtcac caccggtacc gacggtatcc ggcagcagca tacggttcag
    17101 ccaggagtgg attacgccgt cacccggacg cagcgacaca ccaccacggt tcataatgaa
    17161 gtccggcagc gtgtggtgcg tgttcacgtc aactggcttc ggatacgccg cggtatggca
    17221 gaaagactgc atcaccaggt cagccgagaa gcccaggcac gccaggtctt tcagttcatc
    17281 acgggtcatc gggccggtgg tgtcctgtga acctacagaa gtcattttcg gttcgcagta
    17341 cgcgcccgga cgaatacctt tcacaccgca ggcacgacct accattttct gagccagcga
    17401 gaagccgcga tcgctctcag cgacatcttt cgcctgacgg aacacatcac tgtgcggcag
    17461 accaagtgct tcacgcgctt tggtggtcag gccacgcccg ataatcagcg gaatacggcc
    17521 accagcacgc acttcatcaa tcagcacgtc ggttttcagt tcgaaggtcg ccagcagttc
    17581 gccggtttcg tggttacgca cttcaccttt gtacgggtaa acgtcaatca cgtcgcccat
    17641 gttcaggtta gagacgtcga cttcgattgg cagtgcaccc gcgtcttcca tcgtgttaaa
    17701 gaagatgggt gcaattttac cgccgaggca caaaccaccg ccgcgtttgt tcggcacatg
    17761 tggaatatca tcgcccataa accacagaac ggagttcgtg gcggatttac gcgatgaacc
    17821 cgtacccaca acgtcaccga cgtacgccag cgggaaacct ttctgttgca gagcttcgat
    17881 ttgcttgatc gggccaacaa cacctggctg gtctggctca ataccttcac gggcgttttt
    17941 cagcatcgcc agcgcgtgca gtgggatatc cgggcgcgac cacgcatccg gtgccggaga
    18001 aaggtcatca gtgttggttt cgccagtgac tttgaagacg gtaacggtca gtttttcagc
    18061 cagcgccggg cgattcagga accattcggc atccgcccag gactgcatta cctgcttcgc
    18121 atattcgttg cctgctttgg ctttctcttc tacgtcatag aagttatcga acatcagcag
    18181 tgtgtgagaa agtgctttgg cagcgatcgg tgccagtttg gcatcatcca gcgcgtcgat
    18241 cagcggatga atgttgtaac caccctgcat ggtacccagc agttcgatgg ctttttccgg
    18301 agtcagcaga ggggatttgg cttcgccttt agcgatagca gccaggaagc ctgctttgac
    18361 ataggcggct tcatcgacgc cagggggaac acggttggtt aatagatcta acaggaattc
    18421 ttcttcgccc gcgggcgggt ttttcagcag ctctacaagt gcggccattt ggtttgcatc
    18481 caggggtttg ggcgcaatcc cctcagcggc acgctcagct acgtgcttac ggtattcttc
    18541 tagcacgacg gttctcctcg ctctcattgt catagtgcgg caggcgattc tcttcacgct
    18601 cctgtgagac agcagtttgt agggtaaatg cccagtaccg caacgggcag aatagcagga
    18661 ttttgacgac gtgttaatct gtttacaaaa aagcaacata aaaaagtggc tgaatcgtta
    18721 aggatggtct agagattgtt tccctccgta aaaacttcgt taattcgcat ggtgataatc
    18781 acctttctca acgaatccca agattagtca aaatttaaac taccgcctct ttatactcgg
    18841 attaacagca cctgcgggtg gcagttcgcc gaccattgcg atttccttga gatccgaatt
    18901 atgaaaatga ctttgccgtt taaaccccat gtgctggcac taatttgcag tgccgggctt
    18961 tgtgccgcct ctgccgggct atatataaaa agccgcacag tggaagcgcc tgtggaaacg
    19021 caatcgacac aacagactgc gcctgacatc accgcagtta cgcttcctgc aacggtttcc
    19081 gcaccgcccg taacgcccgc cgtcgtcaaa tccgcattca gcactgcaca aatcgatcaa
    19141 tgggttgcgc ctgtcgcgct gtaccccgat tctctgcttt cacaagtgtt aatggcatca
    19201 acctatccgg caaacgttgc tcaagcagtg caatggtcgc acgataatcc acttaaacaa
    19261 ggcgatgctg ctattcaggc ggtatctgac cagccgtggg acgccagcgt taaatcactg
    19321 gtggcctttc cacaattgat ggcattgatg ggcgaaaacc cgcaatgggt gcaaaacctg
    19381 ggcgatgctt ttctggcgca gccgcaggac gtgatggact cggtacagcg attgcggcaa
    19441 ctggcgcaac aaaccggctc gctgaagtca tcaaccgaac agaaagttat taccacaacg
    19501 aagaaagctg taccggtaaa acagacagtc acggcacccg tcataccatc caataccgtt
    19561 ttaactgcca accccgtcat tacagagccg gcaacaaccg tcatttccat tgagcccgcc
    19621 aatcctgatg tggtctatat tcccaactac aacccaaccg tggtttacgg gaactgggcc
    19681 aatactgcgt atccgccggt ttatctgcca ccaccagccg gagaaccgtt tgttgacagc
    19741 tttgtgcgcg gattcggcta tagcatgggc gttgctacca cgtacgcact atttagcagc
    19801 atcgactggg atgacgacga tcatgaccat catcatcatg acgatgatga ttatcatcac
    19861 cacgatggcg gtcatcgtga cggtaatggc tggcaacaca acggcgacaa catcaatatc
    19921 gacgtcaaca atttcaaccg tatcaccggt gagcatctta ctgataagaa tatggcatgg
    19981 cggcacaatc caaactaccg taatggtgtg ccctatcatg atcaggatat ggcaaagcgg
    20041 tttcatcaaa ccgatgtcaa cggcggaatg agtgccacgc agctacctgc tccaacacgc
    20101 gacagccagc gtcaggcggc agcaagtcag tttcagcaac gaacacacgc cgcccccgtc
    20161 attacacgag atacccaacg tcaggcagcg gcacagcggt ttaatgaagc tgaacactat
    20221 gggagctatg acgacttccg cgacttcagc cgtcgccaac cactgaccca gcaacaaaag
    20281 gacgccgctc gtcagcgtta tcaatcggcc tctcctgagc agcgccaggc ggttcgcgag
    20341 aaaatgcaga ctaacccaca gaaccagcag cgaagagagg cagcgcgtga gcgtattcag
    20401 tctgcaactc ccgaacagcg ccaggtgttt aaggaaaaag tacagcagcg cccactgaac
    20461 caacagcaac gtgataacgc ccgccagcgt atacaatcgg catcacctga acaacgtcag
    20521 gtttttcggg aaaaagttca ggagagccgc ccacaacgtc taaacgacag taaccatact
    20581 gtcaggctga ataacgagca acggtcagca gtacgcgaac gtctctctga gcgcggagca
    20641 aggcgacagg aaaggtaaat tgcagacgta aaaaaagcgg cgtggttagc cgctttttta
    20701 attgccggat gttccggcaa acgaaaaatt acttcttctt cgctttcggg ttcggcaggt
    20761 cggtaatgct accttcgaac acttctgccg ccaggcccac agactcgtgc agagtcgggt
    20821 gcgcgtggat ggtcagtgcg atgtcttcag catcacaacc catttcgatt gccagaccga
    20881 tttcacccag cagctcaccg ccgttagtac cgacaatcgc accaccgatc acacggtgag
    20941 attctttgtc gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg atagcacgac
    21001 cagaagcagc ccacgggaag gtggcggttt catagctgat gcctttctct ttcgcttctt
    21061 tctcagtcag acctacccat gcaacttctg gttcggtata ggcgatagac gggataactt
    21121 tcggatcgaa gtagtgtttt ttaccggcga taacttcagc ggcaacgtga ccttcgtgaa
    21181 cacctttgtg tgccagcatc ggctgaccaa cgatatcgcc gatagcaaag atgtgcggta
    21241 cgttggtacg cagctgtttg tcaacgcgga tgaaaccacg gtcgtccact tccacgccag
    21301 ctttgcctgc gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc agcacggcgt
    21361 cgtaacgctg cggttcagcg ggtgcttttt tgccttccat cgtcacataa ataccgtctt
    21421 ctttcgcttc aacggcggta actttggttt ccagcatcag gttgaatttc ttgctgatac
    21481 gcttggtgaa gactttaacg atgtctttgt cagctgccgg gataacctgg tcgaacattt
    21541 caaccacgtc aatctgtgaa cccagcgcgt ggtatacggt gcccatttcc agaccgatga
    21601 taccgccacc cattaccagc aggcgttctg gtacttcttt cagttccagc gcgtcagtgg
    21661 agtcccagat acgcggatct tcatgcggaa taaacggcag ttggatcggg cgagaacccg
    21721 ctgcaatgat cgcattgtcg aagttgatca cggttttgcc gttctcacct tcaacttcca
    21781 gggtgttagc cccggtgaat ttacccagac cgttgaccac tttgactttg cggcctttcg
    21841 ccataccagc cagaccaccg gtcagctgat tgatcacttt ctctttccaa gtacgaatct
    21901 tgtcaatatc ggttttcggt tcgccgaaga cgataccgtg ttcagccagc gctttggctt
    21961 cttcgataac ttttgctacg tgcagcagtg ctttagaagg gatacagccg acgttcaggc
    22021 aaacaccgcc aagggtgttg taacgttcta cgattacggt ttccagacct aaatcagcgc
    22081 aacggaaggc agcggagtaa cctgcggggc ctgccccaag taccacgacc tgagttttga
    22141 tttcagtact catcatgacc tctatatatt tatctccggc ggtcataccc gtcgtctttc
    22201 aggccgcagc ggcgttagct tcacgccctc accccagtca cttacttatg taagctcctg
    22261 gggattctgg cgcttgctac cttgctgcac ccagaaatcc atagggtatg tgaagtaata
    22321 ccctaaccac caccgggtcg ttctatccgt cggtatttta caaaattgtt aacaattttt
    22381 aaacaacaaa cggcaaccga tttgtctatt cgctaataac ctcaatacat tcatgagatt
    22441 accagaaaaa agccggccgt tgggccggct cttttactta catcaccaga cggcgaatgt
    22501 cagacagcgt gttgttaatg atggtaatga aacgggcacc atcagcaccg tcgatcacgc
    22561 ggtggtcgaa ggagagagaa atcggcagca tcagacgcgg cacgaactct ttaccattcc
    22621 acaccggctc catcgcggac ttggaaacgc cgaggatagc cacttccggc gcgttcacaa
    22681 tcggcgcgaa gtgggtagta cccaggccgc cgatgctgga gatggtgaag caaccgccct
    22741 gcatttcgcc cgcagtcagc ttaccgtcac gcgctttctt agaaatagtc atcagctcgc
    22801 gagacagctc gatgatgcct ttcttgttga cgtctttgaa taccggaaca accagaccgt
    22861 tcggggtatc caccgccaca ccgatgttga tgtatttctt cagggtcaga cgctgaccgt
    22921 cttccgacag cgaactattg aagcgaggca tctgctcaag agctgcagca acggctttca
    22981 tgatgaagac aaccggggtg agcttcacat ccagcttacg tttcgccgct tcttcgttct
    23041 gctgtttacg gaacgcttcc agctcggtga tatcggtttt gtcgaagtgg gtaacatgcg
    23101 ggatcatcac ccagttacgg ctcaggttag caccagaaat tttctggata cggcccagtt
    23161 ccacttcttc gatttcgcca aacttgctga agtccacctt cggccacggc agcatgccag
    23221 ggataccacc gccagtcgcc gccggagctg cttctgcacg tttgatagct tctttcacgt
    23281 aagcctgaac gtcttcgcgc aggatacgac ctttacgacc agtgcccttc actttcgcca
    23341 ggttaacgcc aaactcgcgt gccagacggc ggatcagcgg agtcgcgtga acgtaagcgt
    23401 cgttttcagc aaattcagat ttgccttcgg ctttcgcagc cggagctgct gccggggctt
    23461 cagctttcgc tgccggagcc ggcgctgccg cttcctgttt cgcaggagct gccgcaggcg
    23521 ctgcgccttc aacttcgaag atcataatca gcgagccagt tttcacttta tcgccaacgt
    23581 tgactttcag ttccttcacg acgcctgcaa acggagccgg gacctccata gaagctttat
    23641 cgccttctac ggtgataagc gattgttcag cggcaacttt gtcgcccact ttcaccatca
    23701 cttcggtcac ttcaacttcg tcaccgccga tatccggaac gttaacttct ttcacgccag
    23761 ccgctgatgc aggggccgct gccggagctg cttcctgttt agccgccgga gctgccgcgc
    23821 ctgcttcacc cgcgacttcg aagaccataa tcagcgagcc ggtagacact ttgtcaccca
    23881 cgttcacttt gatctctttc acggtgccag caaacggagc cggaacttcc atagaagctt
    23941 tatcgccttc tacggtgatc agcgactgtt cagcttcaac tttatcgcca actttcacca
    24001 ggatttcggt cacttcaact tcgtcgctgc cgatatccgg aacgttaacg tcttttgccg
    24061 ccgcagccgc gggtgctgct gccggagctg cttctttctt ctcttctgcc tgagcaggtg
    24121 cagcgtctgc tgcaccgtcg gcggaatcga aaatcataat cagtgcgccg gtctgggttt
    24181 tatcgccaac agagactttg atctctttaa cgatacccgc ctgcggagac ggaacttcca
    24241 tagaggcttt gtcgccttct acggtgatca gcgactgttc ggcttcaact ttgtcgccca
    24301 ctttgaccag gatctcggtg atttcaactt catcagcccc gatgtccggt actttgattt
    24361 cgatagccat tattctttta cctcttacgc cagacgcggg ttaactttat ctgcatcgat
    24421 gttgaatttg gcgattgcgt cagcaaccac tttcttatcg atttcgccac gtttagccag
    24481 ttcgcccagc gccgcaacca cgacataaga agcatcaact tcgaagtggt gacgcaggtt
    24541 ctcacggctg tcggaacgac cgaagccatc agtacccagt acgcggtagt cgtcagccgg
    24601 tacgtaagta cggacctgct cagcgaacag tttcatatag tcggtagatg ccactgccgg
    24661 agcgtcgttc atcacctgag cgatatacgg aacgcgcgga gtttccagcg gatgaagcat
    24721 gttccagcgt tcacaatcct gaccatcacg cgccagctcg gtgaaggagg tcacgctata
    24781 aacgtcagaa cctacgccgt aatctttcgc caggatctct gccgcttcac ggacgtgacg
    24841 caggatagaa ccggagccca gcagctgaac tttacctttg ctaccttcaa tggtttcgag
    24901 tttgtagata cctttacgga taccttcctc agcaccttcc ggcattgccg gcatgtggta
    24961 gttttcgttc agcgtagtga tgtagtagta aacgttctct tgtttttcac cgtacatacg
    25021 ctccagaccg tcatgcatga tgacagcaac ttcgtaagcg taagccgggt cgtaagagat
    25081 acagttcggg atagtcagcg actgaatgtg gctgtgacca tcttcgtgct gcagaccttc
    25141 accgttcagg gtggtacgac cggaagtacc gccgatcagg aagccacgcg cttgctggtc
    25201 gccagccgcc cagcacagat cgccgatacg ctggaagccg aacatcgagt aatagatgta
    25261 gaacggaatc atcggcagat tgttggtgct gtaagaggtc gccgctgcca gccaggaaca
    25321 ccctgcgccc agctcgttga tcccttcctg cagaatctga cctttctcgt cttctttata
    25381 gtaagcaacc tgctcgcggt cctgcgggat gtactgctga ccgttcgggc tgtaaatacc
    25441 aatctgacgg aacagacctt ccataccgaa agtacgcgct tcgtcggcga tgatcggtac
    25501 cagacgatct ttgatcgact tgttcttcag cattacgttc agggcacgaa cgaaagcgat
    25561 agtggtagag atctctttgc tctgctcttc cagcagcgcg ccgaagtctt gcaggctcgg
    25621 cagctcaagc ttctcggtga agttcggctg acggcttggc agataaccgt gcagtttctg
    25681 acgctgtgcg tgcagatagg tatgctcttc agaaccttcc gggaaggtga tgtacggcag
    25741 tttttcgata tctgcatcag acaccggcac attgaaacgg tcgcggatgt gacgcacgcc
    25801 gtccatgttc attttcttaa cctggtgcgc gatgttttta ccttcagccg cgtcgcccat
    25861 gccgtaacct ttaatggtat gagcaaggat tactgtcgct ttgcctttgg tttcctgcgc
    25921 tttcttgaat gcagcgtaga ttttcttcgg atcgtgacca ccacggttca gtgcccagat
    25981 ctgctcgtca gtccagtctg caaccagtgc tgcggtttca ggatatttac cgaagaagtg
    26041 ttcacgaacg tacgcaccat ctttcgattt gaaggtctgg tagtcgccgt caacggtttc
    26101 gttcatcagc tggatcagtt taccgctggt atctttacgc agcagttcat cccaacggct
    26161 accccacatc actttgataa cgttccagcc agcaccttcg aagatgcctt ccagttcgtt
    26221 gatgatcttg ccgttaccgg tgaccgggcc gtcaagacgc tgcaggttac agttgataac
    26281 gaagaccagg ttatccagtt tttcacgggt agcgatggtg atcgcacctt tggattccgg
    26341 ttcgtccatt tcaccgtcgc cgaggaacgc gtaaacggtc tgtttagagg tatctttcag
    26401 gccacggtgt tccagatatt tcaggaattt agcctggtaa atagcaccaa tcggccccag
    26461 acccatagat acggtcggga actgccagaa ttccggcatc agtttcgggt gcggatagga
    26521 agagaggcca ttgccgtgaa cttcctgacg gaagttatcc agctgctctt gagtcagacg
    26581 accttccagg aaagcacgag cgtacacgcc cggggagatg tggccctgga agtaaaccag
    26641 gtcgccgcca tcctgctcgt tgcgtgcacg gaagaagtgg ttaaagcaca catcataaat
    26701 ggttgcggaa gactggaagg acgccatatg gccgcccagt tcgaggtctt ttttcgacgc
    26761 acgcagcacc gtcatgatgg cgttccagcg gatagctgaa cgaatacggc gttccagttc
    26821 cagattaccc ggatactccg gttgttcttc aacggggatg gtgttgatgt agttgctgat
    26881 acctgtgcct gcggctacgt ttacaccgcc tttgcgggct tcagcaagca gttggtcgat
    26941 cagatactga gcacgctcaa caccttcttc acggatgacc gattcgatcg cctggagcca
    27001 gtcgcgagtt tcgatcggat ccacgtcatt tgggaaacgt tctgacatgg gttattcctt
    27061 atctatctaa taacgttgag ttttctggaa cctgtcccat tgaactctcg ccggaaagct
    27121 caataagaca ggttctacgt ttagttgccg cgcttttata tacgcttgat ttacaacatc
    27181 ttctggataa tttttaccag aaaaatcact aattctttcg ttgctccaga cgacgcagag
    27241 aacgctcacg gcggctctct tcacgacttc tgtcgagcaa aatttcttcg ataaaggcca
    27301 gatggcgatg cgatgcttcg cgcgcttctt ccggcttacc ggccataatc gcttcaaata
    27361 tgcgggtgcg gtgactactc accagcggca gcatctcgcg acgcgaatag agcaattcga
    27421 agttctggcg gacattctgg gccaacatcg gctccataca ccttagcaga tgaagcagaa
    27481 ccacattgtg ggccgcttcg gtgacggcaa tctgatactg gagtacggcg tttgattccg
    27541 cgtccagatc gccagactgc tgcgccagct ctatggcgtg gtggagttca cggatgcgtt
    27601 ccttgtcttc atcggtacta cgcagcgcgg cgtaataagc ggcgatacct tccagggcgt
    27661 gtcgtgtttc gagcaagtca tactgtgatt caggatggtc ggagagcagc tccaccagcg
    27721 gatcgctgaa gctttgccat aggctgctct ggacaaaagt gccgccaccc tggcgacgaa
    27781 gcaacaagcc cttcgcttcg agacgttgaa tcgcctcacg caaggaggga cgggagacgt
    27841 cgaactgttt tgccagttcg cgttccggtg ggagtttttc gcccgggcgg agagtgcctt
    27901 cgagaatcaa aaactccagt tgctgctcaa tcacatcgga gagttttggt tggcggattt
    27961 tgctgtaggc catgagttcc tgtcttaagc cacttgccga agtcaattgg tcttaccaat
    28021 ttcatgtctg tgacgctaaa gtaacaaagt attcacctta tgtccataca ggttttgatt
    28081 gaaatcatga aactgtgcac attttaacaa cttgacatat ataacgtttc aaaattgtaa
    28141 ctatgcacaa atgtagactt tacgtaggaa agggattttt taacgattat taaactataa
    28201 aaatccgaat tgaaccgatt cacttaccaa ttttttgatt tttaagtcaa taaaaacgaa
    28261 tttaaattca ttctgcatat tgagaggggt tgaggctgag ctttacaaac ggtttctttt
    28321 taagcaactc atcttcaacc atgcataaag cgggtgcatt cgctgccgca taccattatt
    28381 cttgatctga cggaagtctt tttgtaacaa ttcaaacttc tttgatgtaa aaaaattaat
    28441 acaacaaacg gaattgcaaa cttacacacg catcactgcg tagatcaaaa aaacaatcac
    28501 cgcacgaggt ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc gcggccttaa
    28561 aaaccgccat attcagctta tcgcgctggg tggcgcgata gggacagggc tattcctggg
    28621 tagcgcctcc gtaatacagt ccgcagggcc agggattatc ctgggttacg ccattgctgg
    28681 ttttatcgcc tttctgatca tgcgtcagct gggtgaaatg gtggtcgaag aacctgtcgc
    28741 aggctccttt agccactttg cttataaata ctggggcagt tttgccggtt tcgcctctgg
    28801 ctggaactac tgggtactgt acgttttagt tgccatggca gagctgactg ccgtgggtaa
    28861 atacattcag ttctggtatc cggaaatccc aacctgggtt tctgccgccg tattctttgt
    28921 ggtgattaac gccatcaacc tgaccaacgt taaagtgttt ggcgagatgg agttctggtt
    28981 tgccattatc aaagttattg cgatagtagc gatgatcatc ttcggcggct ggctgctgtt
    29041 cagtggcaac ggcggcccgc aggcgagcgt tagcaacctg tgggatcaag gtggtttcct
    29101 gccgcacggc ttcaccggac tggtgatgat gatggcgatt atcatgttct cgttcggtgg
    29161 tctggaactg gtggggatca ccgcagcaga agctgataac ccggagcaaa gtatcccgaa
    29221 agcgactaac caggttatct accgcatcct gattttctat attggttcgt tagccgttct
    29281 gctctcactg atgccgtgga cccgcgttac cgccgatacc agtccgtttg tgctgatctt
    29341 ccacgagtta ggcgatacct ttgtggcgaa tgcgctgaac atcgtggtac tgactgcggc
    29401 gctctccgtg tacaacagct gcgtatattg caacagccgt atgctgtttg gtctggcaca
    29461 acagggtaat gcgccaaaag cgctggcgtc tgtcgataaa cgcggcgtac cggttaacac
    29521 cattctggtg tctgcgctgg ttacagcatt gtgcgtattg attaactatc ttgctccgga
    29581 atccgcattt ggcctgttaa tggcactggt ggtatccgca ctggtgatca actgggcgat
    29641 gatcagtctg gcgcatatga agttccgtcg cgccaagcag gaacaaggcg tggtaactcg
    29701 cttccctgct ctgctttatc cgctgggtaa ctggatctgc ctgctgttta tggcggcggt
    29761 actggtgatt atgctgatga ccccaggaat ggcgatttcg gtatacctga tcccggtatg
    29821 gctgatcgtg ttaggtatcg gctatctgtt taaagagaaa accgccaaag ccgtaaaagc
    29881 gcattaatct ctctacgccc tcacccatgc agggtgaggg caataatctt tacaccaacg
    29941 ctccgtaaat cgtcagtagc gcaatcacca ccacgaccac gaacgaggtt ttcttcgcca
    30001 ttgaaaccgc tgccttcggc gtctccacct tatcgacatg cggttcacgc gccagagaga
    30061 actgcgccag acgcgttaac acctgatact gcgaagtatg gaagtcaccc agcgaagcaa
    30121 accaggccgg taacgctttc tccccatgac cgatcaaggc atataccaca cccgcaagac
    30181 gaaccggcac ccaatccagt acatgaagca cggcatcaat gccggactgt aaacgatgat
    30241 gcggcgtctg gtatcgcgcc agccagtatt gccatgcacg caaaaacgca taccccatca
    30301 gcgtaacggg tccccaggtt ccccccacaa tcaaccagaa cagcggtgca agataaaaac
    30361 gaaagttaat ccacagcaat gcattttgca gctcacgcaa atactcacgt tcgtcgcagc
    30421 ctgccgggac gccgtgaatc atggtgagtt cgccagccat cgtggcacgg gcatggctat
    30481 cattacgtga agcagctgtc agataagcat gataatgaag acgaacttta cctgcgccaa
    30541 tacacagcaa accaatcagc agccacacca gcagcgtggg aacgttgaac aatactccct
    30601 gcaatgcgcg taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg cctaacgtgc
    30661 gcccgagaga aaaatgtttc acccgccgaa agaacgcttc aagacgatga tcaagctgcc
    30721 agtgctcgcc caacttaaac aggcgctcga aaattaacac cagtaaggtt gtaaatagcg
    30781 tcatgttgtc tccttgctga ccagcgcacg aaaccgtgcc caatcaaatg caggaccggg
    30841 atcggttttc cgatccggcg caatatcaca atggcccgtc atgttattag cgatatcggg
    30901 atagcgatct atcagtgccc gcgtaaccgc cgcaagctgt tgatactgcg catcggtata
    30961 cgccagagta tcggtgcctt caagctcaat cccaatagaa aaatcattgc agcgttcacg
    31021 cccctgatac tgagagactc ccgcatgcca ggcacgttta tcgaaaggaa catactggac
    31081 tatttcacca tcacggcgaa tcaaacagtg agcggagacg cgcaaatggg cgatctcagc
    31141 aaagaaagga tgtgcctgcg gatcaatagt tccagtgaat aatgcgtcga tccacggacc
    31201 gccaaactcg cctggcggca ggctaatatt gtgcaccacc agcagggtgg gtgtttcgtc
    31261 atccgggcgg caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca gccacccctg
    31321 ttctaacaac atgcaggatc tccttatatg tggtgctaat acccggttca gagtagcatg
    31381 tttctacctt atgattcgtt agctatctgg agttttaaca tgccgcctcg ccgctataac
    31441 cctgacaccc gacgtgacga gctgctggaa cgcattaatc tcgatatccc cggcgcggtg
    31501 gcccaggcgc tgcgggaaga tttaggcgga acagtcgatg ccaacaatga cattacggca
    31561 aaacttttac cggaaaactc tcgctctcat gccacggtga gcacccgcga gaatggcgta
    31621 ttttgcggca aacgctgggt tgaagaggtg tttattcaac tggcaggcga cgatgtcacc
    31681 ataatctggc atgtggatga cggcgatgtc atcaacgcca atcaaccctt gttcgaactt
    31741 gaaggcccat cccgcgtgct gttaacgggc gaacgcactg cgcttaattt tgtgcaaacc
    31801 ctttcaggag ttgccagtaa ggtacgccac tatgtcgaat tactggaagg caccaacacg
    31861 cagttgttgg atacgcgcaa aaccttacct ggcctgcgtt cagctctgaa atacgcggta
    31921 ctttgcggcg gcggagcgaa tcaccgtctg gggctttctg atgccttcct gatcaaagaa
    31981 aatcatatta ttgcctccgg ctcagtgcgc caggccgtcg aaaaagcgtc ctggctgcac
    32041 ccggatgcgc cagtagaagt cgaagtagag aatctggaag aacttgatga agccctgaaa
    32101 gcaggagccg atatcatcat gctggataac ttcgaaacag aacagatgcg cgaagccgtc
    32161 aaacgcacca acggcaaggc gctactggaa gtgtctggca acgtcactga caaaacactg
    32221 cgcgaatttg ccgaaacggg cgtggacttt atctccgtcg gtgcgctaac taaacacgta
    32281 caagcactcg acctttcaat gcgttttcgc taattgctga cagatttatc cgctccatcg
    32341 cgggcggata atctcccctt ccccaacttt cttcgtaacg cctcgcaaat ttcttgtctt
    32401 ttgctgcaac gaaataaaaa acctcggaag ctgctttccc gtttcgtttt ttgcccctcg
    32461 cttgtgctga tggcgtttgc caaagtagcg ccaaccaaat caaggagcga aacagatgga
    32521 caagcaacgc ggttttacac ttatcgaact gatggtggtt attggcatca ttgccatttt
    32581 aagcgccatt ggtattcccg cttatcaaaa ctacctgcgc aaagctgcac tcaccgacat
    32641 gctacaaacc tttgtgcctt accgtacagc cgtagagttg tgcgcgctgg aacatggtgg
    32701 attagatacc tgcgacggtg gcagcaatgg cattccctcg cctaccacca cccgctatgt
    32761 ttcagccatg agtgtggcaa aaggcgtggt gtcgctgacc gggcaagaaa gtctcaatgg
    32821 actaagcgtc gtcatgacac cgggttggga taacgcaaac ggcgtcaccg gctgggcgcg
    32881 caactgcaat attcaaagtg acagcgcatt gcagcaagcc tgcgaagatg tcttccgctt
    32941 tgatgacgcc aactaaggag cggcaatgaa tattccacag ctcaccgccc tgtgtctgcg
    33001 ttatcaggga gtcttgctgg atgccagcga agaggtggtt catgttgcgg tagtcgatgc
    33061 accttcgcat gagttgctgg acgcattgca tttcgctacc accaaacgta ttgagatcac
    33121 ctgctggacg cgccaacaaa tggaaggcca cgccagtcgc acacaacaga cattgcccgt
    33181 agctgttcag gagaagcatc agcccaaagc agagttgctg actcgaacgt tacaatctgc
    33241 gctggaacaa cgtgcgtctg atattcatat cgaaccagcg gacaatgcct accgcatccg
    33301 cttgcgtatc gacggcgtat tgcatccttt accggatgtt tcaccggatg ccggagtcgc
    33361 attaaccgcc agattaaaag tgctgggaaa cctggatatt gcggaacatc gcctgccgca
    33421 ggacgggcaa ttcactgtcg aactggcagg aaacgccgtc tcatttcgta ttgcgacctt
    33481 accatgtcgg ggtggtgaaa aggtggtatt aaggttgtta cagcaggtga accaggcact
    33541 ggatgttaac acgctgggaa tgcagccgtt acaactggcg gactttgctc atgccttgca
    33601 acaaccacag ggactggtgc tggtaactgg ccctacaggc agcggcaaaa cggtcacgct
    33661 ttatagtgcc ctgcaaaagc tgaataccgc tgacattaat atttgtagcg tcgaagatcc
    33721 ggttgagatc cccatagccg gactaaacca gacgcaaatc catccgcgtg ccgggctcac
    33781 ctttcagggc gttttgcgtg cgttattgcg ccaggatcct gacgtcatca tgatcggaga
    33841 gatccgctat ggctttaacg gcagaaattg ccattaaagc cgcgcaaacc ggtcacctgg
    33901 tgttgtctac cctacacact aattccacct gcgaaacgct ggtacgttta cagcaaatgg
    33961 gagtcgcccg ctggatgctc tcatcagcgc ttacgctggt aatagcccag cgtctggtac
    34021 gtaaactttg cccacattgt cgccagcagc aaggggagcc catccatatt ccagtcaatg
    34081 tatggccgtc gccgctgccc cactggcagg cacccggttg tgtacattgc taccacggtt
    34141 tttatggtcg tacggcctta tttgaagttc tgcccataac gccggtcatt cgtcagctta
    34201 tttccgctaa taccgacgtt gaatcgctgg aaacgcacgc acgacaggcg ggtatgcgta
    34261 cgctttttga aaacggctgc ctggccgtgg agcaaggctt aaccaccttt gaagagttaa
    34321 tccgcgtact ggggatgccg catggcgagt aagcaactct ggcgctggca tggcataacc
    34381 ggcgacggca atgcgcaaga tgggatgcta tgagcagaga gccgtgcttt gctgctcatg
    34441 gcactacagc aacagatggt taccccactt agcctgaagc gaatcgccat caattctgcg
    34501 cagtggcgag gagataaaag cgcggaagtc attcatcaac tggcgacgct actcaaagcc
    34561 gggttaacgc tttctgaagg gctggcactg ctggcggaac agcatcccag taagcaatgg
    34621 caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt ttccaatgcc
    34681 ttattaccct ggtcagaggt atttccgcca ctctatcagg cgatgatccg cacgggtgaa
    34741 ctgaccggta agctggatga atgctgcttt gaactggcgc gtcagcaaaa agcccagcgt
    34801 cagttgaccg acaaagtgaa atcagcgtta cgttatccca tcatcatttt agcgatggca
    34861 atcatggtgg ttgtggcaat gctgcatttt gttctgccgg agtttgccgc tatctataag
    34921 accttcaaca ccccactacc ggcactaacg caggggatca tgacgctggc agactttagt
    34981 ggcgaatgga gctggctgct ggtgttgttc ggctttctgc tggcgatagc caataagttg
    35041 ctgatgcgcc gaccgacctg gcttatagtg cggcagaaat tgctgttacg catcccgatt
    35101 atgggttcac tgatgcgggg acaaaaactc acgcagatct ttacgattct ggcgctgaca
    35161 caaagtgcag gcattacttt tttgcagggc gtagagagcg tcagagaaac aatgcgctgc
    35221 ccgtactggg tgcaacttct gacacaaatc cagcacgata tcagtaacgg tcatcccatc
    35281 tggctggcgc taaaaaatac cggggagttt agcccgctct gtttgcaatt agtgagaaca
    35341 ggagaggcat ccggctcgct ggacctcatg ttagacaacc tcgcccatca tcatcgggat
    35401 aacacaatgg cgctggcgga taacctcgca gccttactgg aaccgacgtt gctgatcata
    35461 acgggaggaa ttatcggtac gctggtggtg gcgatgtatc tgccaatttt ccatttaggc
    35521 gatgcgatga gtgggatggg ataacgctgg cgtgttgctc cacaccagcg ttggaagatt
    35581 acaggttgtt gaagatgcgg ttttcttgtt cctgcacacg gataaatgtg gtgcgcttgg
    35641 tcagttcttt caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg ccgcccaaaa
    35701 tatcacgcgc ggtattttca accgggcctc gcagcggcag cttaacggtt ttaccttctg
    35761 ctgcgcgata ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg gagctcatgc
    35821 cgtagaacag cataaatttc tcgccgttct cctcaacgat gcgaccgccg ctctcttcgt
    35881 ggcccgccag catgccgcca agcatgacga aatcggcacc gccgccaaag gcttttgcga
    35941 catcgcccgg cgtggtgcag ccaccgtcgc tgacgatcat tccgcccaga ccgtgcgccg
    36001 catcggcaca ttcgattacc gcagaaagct gcggataacc gacgcctgtt ttgacgcgag
    36061 ttgtacaaac agaacctggg ccaatgccaa ctttaacgat atcagcaccg gagaggataa
    36121 gctcctcaca catttcacca gtcactacgt tgccagcaca aatggttttg gtcggccacg
    36181 cttcacgcgc tttcgcaacg aactgcacga agtgttcgga ataaccattc gccacatcaa
    36241 tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg tttagttttt tcgaaatccg
    36301 catcagacgt accggtagaa accatcacat gtttcagcac atcagcggaa gaattgttga
    36361 taaacgcttg ccactcttcg acagaatagt gtttatgcac agcagtcaaa atatcaaaag
    36421 aagccagcgc agaggccata gaaaatgtac ctacggtgtc catatttgcg gcgataatcg
    36481 gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa ttgacgttcc agttcaacat
    36541 cggaacggct tttaagagtg gagcgtttag ggcggatgag aacgtcttta aaacctaact
    36601 tcagatcttc ttcaatacgc atgtgcggat tcctggggtt aatggctata agggtaagag
    36661 cgcaactcca gtgacgctat catacgcact aatcaatgcg gcgcaagact gcgaaattgt
    36721 ctttttttac gctacaatcc cataaattta cctggtaaat agtgagcgaa cgtttcatca
    36781 atttcgcttt cgtgcactca tttttaacgt tttgataatc atgatgaatt cccgggaata
    36841 taaattatga ggtatatagt tgccttaacg ggaggcattg gcagtggcaa gagtaccgtt
    36901 gccaatgcgt ttgctgatct cggaattaac gtcattgatg ccgatattat tgcgcgtcag
    36961 gtggttgaac caggtgcacc tgcgctacat gccattgctg atcactttgg cgctaacatg
    37021 attgctgctg atggaacatt gcagcgccgg gccttgcgcg agcggatctt cgccaacccg
    37081 gaagagaaaa actggcttaa cgccctgctg catccgctga ttcagcaaga gacgcaacac
    37141 cagatccagc aagctacttc cccctatgta ctgtgggttg tgccattgct ggtagaaaac
    37201 tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga aacgcaactt
    37261 aagcgcacca tgcagcgcga tgatgtaact cgcgagcatg tcgaacaaat ccttgctgct
    37321 caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg tcattgataa taacggcgca
    37381 ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac actatttgca gcttgcgtcg
    37441 cagtttgtct cacaggaaaa accgtaatgc agacccaggt cctttttgaa catccactaa
    37501 atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat tcagcaactc accgttaatt
    37561 tacccatcgt tgaccacgct ggcgcgctgc atttcttccg taatgtcagt gaattactgg
    37621 atgttttcga gcgcggcgaa gtccgcactg agctgttgaa agaacttgac cggcagcaac
    37681 gtaaactcca gacctggatt ggcgtgcctg gcgtggacca gagccgtatt gaagcattaa
    37741 ttcagcagtt aaaagcggcg gggagcgtat taatttccgc gccgcgtatc gggcaatttc
    37801 tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact gagcatccca ggcggctgtt
    37861 gcagctttga tttacctaca ttgcacattt ggctgcatct accccaggcg cagcgcgaca
    37921 gccaggtaga aacctggatt gccagcctga acccgctcac ccaggcactt accatggtgc
    37981 tggatttaat tcgccagtcg gcccccttcc gtaaacaaac cagtctgaat ggtttttatc
    38041 aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat tcacagcttt
    38101 atccgcaaat ttccggacat aagagccgtt ttgccattcg ttttatgccg ctggacactg
    38161 aaaacggaca ggtaccggaa cgtctggatt tcgaactggc ctgttgctaa ggagtaaaaa
    38221 tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggtg tggggtgaaa
    38281 tcagcccgtt tcggccattt tgctccaaac gttgtcagct gatcgacctc ggagaatggg
    38341 ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat gactggagcg
    38401 aagagccaaa gcagtgacat ttgtctgatg ccgcacgtag gcctgataag acgcggacag
    38461 cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta tccgacctac
    38521 agacgcttaa gcttcgcaat taccggttca ttggctggcg gaaaatcatc ggcattaaga
    38581 ccgactagcg acatccactc accgggttgc ccttctttac cccacggctc cccttcccag
    38641 ctttcgacca gccagaacca cagagttata tgcctgtccg ggaattcata ttccagtttt
    38701 tcaaatagcg aaaaatgttg tggggtaatc ccgacttctt cctgaagttc acgcactacc
    38761 gcctgttccg gcgtttcgcc catttcaatt ttaccgccgg gaaactccag tttattcgcc
    38821 atgtgcgcat ctgctgcgcg acgcgttata aagatttcat tgttctcgtt gcgaataata
    38881 cctaccgcaa tttgcagctt tttcattgtc ttaaacctat aaaaaaggcg cagaatcctg
    38941 cgccttttac ttcaactgtt agcttttatt gcaggcgacc atggcactgc ttgtattttt
    39001 taccagaacc gcacgggcaa ggatcgttac gtcctacttt gcgctcaccg gtttgcgccg
    39061 ccagtgcagc tgcggctgca gagtcgtcat cctgatggct aagctgctgc atttgcgcta
    39121 aacgctcggc ttccatacga cgctgttgtt ccagctcctc aacctcttca ggcatacgta
    39181 cctggacttt gctcagcgtg ctgataactt catattttaa cgactccagc atcgctgcaa
    39241 acatggagaa cgattcacgt ttgtattcct gcttcggatc tttctgtgca tagccacgca
    39301 ggtggatacc ctgacgcaga tagtccatcg ccgctaggtg ctctttccac agagagtcga
    39361 gagtttgcag catgacgcct ttttcgaagt gacgcatcat ctcagcacca accacttctt
    39421 ctttacgctg atacacttcg atggactgcg ccagaattcg ctcacgcagc gtctcttcat
    39481 gcagttctgg ttctttatcc agccactcgg caattggcaa atcgaggtcg aaatcgttct
    39541 tcagacgttc ctgcagcccc ggaatatccc acatttcttc cagtgactgt ggcggaatgt
    39601 aggcatcaat ggtcgctttg aacacatctt cacgaatgct gttgatggtt tcgctcacat
    39661 cgctgacatc cagcagttcg ttacgctggg agtaaatggc gcgacgctga tcgttagcca
    39721 cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt acggctttca actttacgct
    39781 gggcgttggc aatcgcttta gtcacccacg ggtgttcaat ggcttcgcct ggcttcatac
    39841 ccagtttacg catcatgccg gatactcggt cggaagcaaa aatacgcatc agcgcatctt
    39901 ccatcgacag gtagaaacgg gaagaaccag catccccctg acgaccagaa cgaccgcgca
    39961 actggttatc gatacgacgg gattcgtgac gctcggtacc gatgatatgc aggccacctg
    40021 cttccagtac cgcatcgtga cgtacctgcc agtcggcttt aattttttca atttgctctg
    40081 cggtcggatt ttccagcgcg gcaacttctg cctgccagct accaccgagc acaatatctg
    40141 taccacgacc cgccatattg gtcgcgatag tcaccgcagc cggataacct gcctgagcaa
    40201 caatcgccgc ttcgttggcg tggaatttgg cgttcaggac gttgtgctta ataccggctt
    40261 tggtcagttc gtttgacacc agctccgatt tttcgatgga gatagtaccc accagcaccg
    40321 gctggccttt cgcagtacgt tctttgatat cttcaatgat cgcctgaatt ttttccgctt
    40381 cagtcatgta gaccaggtcc ggcagatctt tacgaatcat tggacggttg gtcggaacaa
    40441 cgacggtatc cagcttgtag atggagctaa attcgaaagc ttcggtatca gcagtaccgg
    40501 tcatccccgc cagtttttca tacagacgga agtagttctg gaaggtgatc gaagccagcg
    40561 tttggttttc gttctggatc tgcacacctt ctttcgcttc cacagcctgg tgcagaccat
    40621 cggaccagcg acggccctgc atggtacgac cggtgtgttc gtcaacgatg ataacttcac
    40681 catctttaac gatgtagtcg acgtcgcggg taaacagcgc atgagcgcgc agcgccgccg
    40741 ttacgtggtg catcagcatg atgttggccg gagagtacag agactcccct tcatccatga
    40801 tgccctcttt caccagcagt tcttcaatca gcaccagacc acgttcggtc aggttcacct
    40861 ggcgagattt ttcgtccacc gagaagtggc cttcgccctg gaaggtttcg gagtcttctt
    40921 tttcctgacg gatcaggtgc ggaataattt tattcacgcg tttatacatt tccgagctgt
    40981 cttctgccgg gccggaaatg atcagcggtg tacgcgcttc atcgatcagg atggagtcca
    41041 cttcgtccac cagcgcatag tgcagtttac gctgtacacg ttcttcaggg ctgaacgcca
    41101 tattgtcgcg caggtagtca aagccgtatt cgttgttcgt accgtaagtg atgtcagctg
    41161 cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag gttgataccg acagtcaggc
    41221 caaggaattc aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg tagtcgttga
    41281 cggtaactac gtgcacgcct ttaccggtta gtgcgttcag gtaagcaggc agcgttgcgg
    41341 tcagggtttt accttcaccg gtacgcattt cggcgatgca gcgttcgtta agaaccatac
    41401 cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac gcgcttactt gcctcacgta
    41461 ccacggcgaa agcttccggg atcagatttt ccagcacttc gcctttttcc agacgtgcac
    41521 gaaactctgc ggttttccct ttcagttctt cgtcggagag tttttccatc tccggttcca
    41581 tggcattgat gatgttgacc actttgcgca tccggcgcag ggtgcgatcg ttacgactac
    41641 cgaaaacttt agttaacaat ttgattagca taataaaatc tcaaacgccc cgcgttgcgg
    41701 agttaataaa atgaagtaaa ggtttattgt tgttaggtga ggcgttgagg gccagcacgg
    41761 atgccttgcg cctggcttat ccagacgggc gtgctgaatt ttgcttgtgg ggtaaaatgc
    41821 gcataatcaa tgcgataacc cttttcagac ggcgtgcctt cctgggtcag cagcgcgctg
    41881 agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca aagactcttc agcaacgggc
    41941 agtgtttgcg gtgccattgc gaaagaaaga tgacggatta ccgtgcgaat ggcatgttga
    42001 tgccagtaat caacggaata gttcgaattc gggcggcgtg tgttcgcttc cagcaaggcc
    42061 aattgaccaa agttaacttt ggctgaaggc tcgtggttgc gggttgtcgc ttttgcgggc
    42121 gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta aactcgccgc aaccatccct
    42181 aataagagat gcggccagaa gtagcgttta ccaaactgtc gccagcgcgt cagtattcca
    42241 ctcacgttat tgccatcccg atcgtattca aacgtattta gcgcacaaat cttatcatta
    42301 aagcctgcag tccacagcac gaatgaagat aagttgtgcg gaaaagtgct taagaaaaca
    42361 gcgttcgcac cgttttttat agctcttata aaattcaaaa aaaaatgact ggttcacctg
    42421 gccggagaga gtgccagatt tgccagtcga attttatacg acagtataaa tgtcgttatg
    42481 ccagtacagc tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc tggaaggtca
    42541 catattccca ggcttcctgt ttcgccagga cagcctgcag cagtttgtta ttcagtgcat
    42601 gaccggattt ataagcggta aatgcaccaa taatattgtg accacacatg aacaagtcac
    42661 cgatcgcatc gagcattttg tgacgcacaa attcgtcttc aaaacgcagg ccgtcttcgt
    42721 tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa gctgccgccc aggcacaaac
    42781 cacgggactg cagatattcg atatcacgca tgaaaccgaa cgtacgcgca cggctgatct
    42841 ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg ctggttgctg gaatcaatag
    42901 ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc ttaaattcag
    42961 cccacttatc gccatcttcg acacgaacag tctctttgat gcgaacaaat tttttggcgc
    43021 agttcaactc gtcgataccg gcgtcaagca gcaggtatac aaacggagcg gcgctgccgt
    43081 ccatgatcgg gatttccggc gcgttaactt cgataacaat gttatcgatg cccaggcccg
    43141 cgagagcagc attgaggtgc tctacggttg aaatccgtac atcatgctcg ttgaccagac
    43201 acgtacagag catggtatca cgcacagatt tggcatcggc cgggaaatct accggtggat
    43261 tcaagtcggt gcgacgatag atgaccccgg tgttggccgg cgcagggcgt aacgtcagag
    43321 tgactttctt gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt ttaagtgtcc
    43381 tttgtttgat catcgtatta tctcgccaaa ttacctatcc aaccgaagtg tactatacat
    43441 tcggcgggcc agtttagcac aaagagcctc gaaacccaaa ttccagtcaa ttcttaatca
    43501 gcttgcttac gcaggaatgc tgggatatcc agataatccg gctctttcgc agtttgcggc
    43561 gcattgtcat tcacgacttt agcaaccggc ttctgctcct gggtcagcgg agccatccca
    43621 tgctgctggt agcgatccat cactggctgc tgaacctgct tattggtcac cagagtgatt
    43681 tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg ttacgcgcag ctcgtcattc
    43741 atatccgggt caagagaagt accgataacc acagtcgcgt tgtcggaagc aaatgcacgg
    43801 atggtgttac ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc cgtgatgtta
    43861 accagcacgc cgcgcgcgcc agacaggtcg atatcttcca gcagcggaga agagatagcc
    43921 atttcagcag cttcttccgc acggtcttca ccgctcgcca cgccagaacc catcattgcg
    43981 tagcccatct cagacattac ggtgcgtacg tctgcaaagt ccacgttcat caaacccgga
    44041 cgagtaatca gttcagcgat accttgcaca gcgcctttca gtacatcgtt cgctgcgcca
    44101 aacgcatcca gcagggagat accgcggccc agaactttca gcagtttgtc gttcgggata
    44161 gtgatcagag agtccacatg cttggacagt tcagtgatcc cctgctccgc gaatgccata
    44221 cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag caacggtcag gatacccaaa
    44281 tcttttgcca cttcagcgac gactggtgct gcacctgtac cggtaccacc acccataccc
    44341 gcagcaataa agaccatgtc tgcaccttcc agcgccgcac gcaatgcatc gcgatcctca
    44401 tcagccgcat tgcgaccaac ttctggatta gcgccagcgc ccagtccttt ggtgataccg
    44461 ctaccgattt gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc atcggtattt
    44521 accgcgaaga attcaacacc ttcaatacgc tcgcgcacca tgtgttcaac agcattaccg
    44581 ccgccgccgc cgacgccgat gactttaatc accgcgtcat tggtaagttc cattggttca
    44641 aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc gtaatcatcg tcggcctcat
    44701 aaaaattaaa actcttttcg cagccaacta ttgagtcgct tgatccacga gccaactgat
    44761 gctgtaacac gtttttctac ttcagcttca ccgttaagat gtgactcttt cccatagtga
    44821 agcaatccca ccgccgtcga ataatacggc tcctgagcat aatccgttaa accggtaatg
    44881 ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc gctgagcaca ggctgcaaga
    44941 ccttcgatct gcgctgcgcc accggttaat acaatgcctg ccgccaggtg atgtttaacc
    45001 ccttgttggc gaagcttttc ctgcaactgc aatatctctt cgttgaccag gttgagcagc
    45061 tcggtatagc gcggctcgat cacctctgcc agggtctgac gttgcagact ccgtggcgga
    45121 cgaccgccta cgctcggcac ttccacgctc tcatcttttc caacgatgga acccagcgca
    45181 caaccgtggc gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa ggcgtaagcg
    45241 atatcactgg tcacgacatt gccagcataa ggaattacct tagtgtggcg caatgcccca
    45301 ccggtataaa cggcgatatc cattgtacca ccaccgatat cgacgacgca gacacccagt
    45361 tcacgttcat cttccgtcaa taccgaataa cttgatgcca gtccggcaaa tatcagttgg
    45421 tcaactttca gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat atcgttgtga
    45481 catgtgatca ggtgcacttt tgcctgcatc cgcacgcccg aaagtcctac cggattcttg
    45541 atcccttcct gatagtcaat cgcatactct tgcgggatca catgcagcac acgatgttca
    45601 tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt ccacatcttc ttgcgtcact
    45661 tcttcttcag aaataggcac cataccaatt tcattctggc agctgatgtg cttaccagaa
    45721 agcgccagat ataccgaaga gatctgacaa tctgccatca attctgcctg gtcaatggcg
    45781 cgttgtacgc acttgaccac ggattcgagg tcgttcaccc cgcctttatc cataccacgt
    45841 gacgggcagc tgcccacgcc aatgatattg accataccgt cgggcagaac ttcccctact
    45901 aaagcggcaa ccttcgcggt accaatctcc agtcctacta ccagttttct gtccgtcgcc
    45961 ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg agtagattcc tctggcggca
    46021 agggcgccca gcctactgcc gctccagagt cataacgcaa atcaacgtag ctaatccgtt
    46081 tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag cgagccaaac
    46141 gtttcatcgt atcgccccgg ccaagattga gcttaatatc gttattcagc gtcaactgcc
    46201 aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt gccagcatct
    46261 gccccatttc gcgatagccc tgcaacactt cattggcgct gccttccggg ccatacagca
    46321 ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct gaaggtgttt ccttccgcgt
    46381 ctaccatatg ttgatcattc caccgcgcaa tcggcacata ttcaaccaga tgaatcttca
    46441 attcatcagg ccactgcttt ctgacgctca cctgcttaat ccacggcagg cgttgttcta
    46501 tttgcgtctg gatgatgttg acatcctggg tcataaaggt acccggctca cccaatgcca
    46561 ggatcgactg ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc aacaccagct
    46621 ttgagagcgg caggcgttgc gcatcttcca tccagcccaa cacgacccag ccgctcacca
    46681 acactgtcgt taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt ccattattgc
    46741 ggcgagaaga aacctcttct tcgctatttc gcgtgttcag agcagcctgc gacatattag
    46801 tccgccagtt ccagaattcg tactaccaac tgcgagaagc tcatacctgc ctgacgtgcc
    46861 gccatcggca ccaggctgtg gctggtcata cccggtgagg tattggcttc cagcagataa
    46921 aactgtccat cgctgtccag cataacgtca atacgtcccc atcctttgca acctaacgtc
    46981 gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttc cagacctgcg
    47041 gggcagaaat actgtgtctc atcagagaga tacttcgcct cataatcata gaaggttccg
    47101 gacggttgaa tacgtattga cggtaaaatt tcttcaccga gtatcgcaac cgtgaactcc
    47161 ggcccactta gccatttttc aatcaatact tcttcatcgt gctgaaatgc caatcttaat
    47221 gcatcttgta gagcattttc tgctactact tttgacattc ccacactgga accttcgcgg
    47281 ctcggcttaa cgataaccgg caaacccaga gcagaaattt ctgctaactg cttatcgctc
    47341 aggccttttt caaactctgc gcgggttaac gctacccacg gcgcgaccgg taaaccggca
    47401 ccttgccaca gcagtttgct gcgtagttta tccattgaaa gcgcagatgc catcactccg
    47461 ctgccggtat aaggcaagcc catcagctca agcatgccct gcagcgtacc atcttcaccg
    47521 ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca tcgacttcag ttgcgtcacg
    47581 tcgacttctt tcgggtcgac aggatacgca tcaataccgc cttcacgcag cccggctaac
    47641 accgctgcgc cagaattcag agaaacttcc cgctcagcgg aagtcccgcc caacaggacc
    47701 gcaattttat cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt tgatttcagc
    47761 taaagaacgg gcaatttttc cgatattacc agccccctga acgagaatca ggtcgttacc
    47821 gattaatacc ggtgccagca tctcggctac ctgcgcaggg tccggcacca gaatgggatc
    47881 aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc ccggaattgg
    47941 cgcttcgcca gccggataca cttccagcat caacagggta tcaacctgcg tcagcacatt
    48001 ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa acagcattac
    48061 taggttttta tccggccagc ctgcgcgcgc cgctttaatg gtggcgtcaa cttccgtcgg
    48121 gtggtggccg tagtcatcga ccagcattgc cgtaccgctt ttaccattca ctggctccag
    48181 cgggaattca ccgaggaaat caaaacggcg accagtcccc tggaagctct ccagcgcacg
    48241 caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag ctgcggcgtt
    48301 cagcgcgtta tgacggcctg gcgcattcag ggtgacgcgc atcggctctt tgtcctggcg
    48361 cagcagcgta aagtgcccct gcgggccaat ctgctgataa tcttctacac gcacgtcggc
    48421 atcttcgctg aagccgtaag tcgtggtctg acgtcccaca cgcggtaaca attcgcggat
    48481 caccggatca tcaacacaca tcaccgcacg accgtaaaac ggcaggttgt gcagaaaatt
    48541 aataaaagtc tgttttaaat tctcaaagtc gccctggtag gtatccatgt ggtcggcttc
    48601 gatattggtg acaatcgcca ccatcggttg cagatgcagg aacgatgcat cactctcatc
    48661 tgcttcggca atcaggtacc gaccatgccc caaacgcgca tgaacccccg ccgcttttac
    48721 cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct gcgtagatgc tggaaaccat
    48781 cgcggtggtt gtcgttttgc cgtgcgttcc tgcaatggcg atgccatgac gaaaacgcat
    48841 taactcagcc agcatttcgg cacgacggat caccggaata cgcgcttcat gagctgcgac
    48901 aatttccggg ttatcggcag aaatggcgct ggaaacaacg accacgctgg catcacgtac
    48961 gttttccggg cgatggttga aataaatcgt cgctcccaga ttcattaact gctgcgtgac
    49021 cggatttggc gctaaatcgg aaccactgat ctgataacct tcattggcca gaacttcggc
    49081 aataccgccc ataccggcac caccgatgcc gacaaaatgt atgtgccgaa cgcgacgcat
    49141 ttcgggcacg atggaacgca gttttgccaa ttgttgtgta ttcattcttt acgccattaa
    49201 cttcttaaat tcatacgatg caaaaggcat cgctacaatt acgcccgggc agcccggctc
    49261 acttcatttg ccactcgctc ggtggcatcc ggaatggatg cagcgcgggc gcgttctgcc
    49321 atggttaata aggtttctcg cgaccacccg gccagggtgt tggcgacagc atccacgcta
    49381 agctgtggct gctcgataat tttggctgcg cccgcttttt ccagcggtag cgcattccag
    49441 tattgctggc ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc tgccgcggcg
    49501 atttcactca ccgttaaagc accggagcgg caaacgacga catccgccca cgcatacgcc
    49561 gccgccatat catcaataaa ttccgtcact ttatgctgcg gttgccccgc ttcggcatac
    49621 gcctgttcaa cggattgttg cgaaccttta ccgctctgat gccagatagt gactgaatca
    49681 cccagtttcg cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc ctgggaacca
    49741 cccactacca gcacacgaac cggaccttca cgtccagcca aacgttgctg cggcaacggc
    49801 agcgccagca catcggtacg caccgggtta cccaccactt ccgcattagg gaaagcgcct
    49861 ggaaacgcct gcatcacttt ggtggcaatc ttcgccagcc atttattggt taagcccgca
    49921 ataccgtttt gttcatgaag tacaaccgga atgcctaacg accacgcggc cagaccacct
    49981 ggacctgata catagccgcc cataccgagt accacgtcag gtttgtacgc tttcataatc
    50041 gcccgcgcct gacgccaggc gttgaagata cgcagcggcg cagctatcag tgcttttata
    50101 ccttttccac gcagaccaga gatacgaatg aaatcaattt cgatgccatg ttttggcact
    50161 aagtccgctt ccatacggtc ggcagtcccc agccagcgaa cttgccaacc ctgagccatt
    50221 agatggtgcg caaccgccag tcccgggaat acatgtccac cggttccgcc tgccatcacc
    50281 attaatcgct ttccttgacc actcatcgtg aacctcgtac aaacgcctgc gctttctcca
    50341 gacgcgtttc ataatcaata cgcaacagca tcatgatggc tgtcgacata atcagtaagc
    50401 tcgaaccacc gtaactgatc agcggcaatg tcagaccttt ggtcggtaac atccccgccg
    50461 ccgcgcctac gttaaccagc gcctggaagc taaaccagat gccaatagaa caggcgagaa
    50521 aaccggaaaa acggtggtca atttctaacg ctttgcggcc aatcgacatc gcgcgaaaag
    50581 cgacgaagaa taccattaaa agtgccagca ccacaccgac ataccccagt tcttcgccga
    50641 taatggcgaa aataaagtca gtgtgcgctt ccggcagata ctccagtttt tgtaccgagt
    50701 tacctaaacc ttgtccccaa agttcgccgc gaccaaacgc catcagcgat tgcgttaact
    50761 gatagccgct gccaaaggga tcttcccacg ggttccagaa tgcggtaaca cggcggatac
    50821 ggtacggttc ggcgagtatc agcaacacaa ccgctgaaat gcccataccg ataatggcaa
    50881 tgaactgcca caatttcgct cccgccagga acaacatcgc cagcgtagtc acaaacaaca
    50941 ccaccaccgt accaaggtct ggctgtgcca gcagtaacac tgccaacacc agaatcacgc
    51001 ccatcggttt caggaagccg cgcaggttat tacgtacttc gtcgccttta cgcaccagat
    51061 agttggcgat ataacaaaac agcgacagtt ttgtcagctc cgcaggctgg atacgcagca
    51121 aaccgagatc gatccaacgc gatgcccctt taaccgagct acccactacc agcacgatca
    51181 tcagcaggat gatagatccg agcagcatcg tggcactgta gcgttgccag aactccatcg
    51241 gcagacgcag cgtaatgatc gccagaatga atgccaggat cagatagacg ccatcacgct
    51301 tcgcgaagaa gaacggatcg ttggttaagc gttgccctat gggcattgac gccgaggtca
    51361 ccatgataaa gccaatcgcc gcaaggccga aggtcagcca cagtaaggtg cgatcgtaca
    51421 tgatcaggct gtcggtatct ttttcccgcg agcccatcac ccagcctttt agcgccgtgg
    51481 agatccagac caggatactg aatcctggca ggcgcggcat tttcaggcga gggagagata
    51541 aacgcatcaa cctaactcct tcgccagacg ggcaaactca ttgcctcgtt gttcaaagtt
    51601 cttgaactga tcaaggctgg cacaggccgg ggagagcaga accatatcgc ccagctgaac
    51661 acgcggagcc agcaagcgca tcgcttgttc catggtttcg gtttgttctg ccacttccgg
    51721 gcgtagcgcc gccagctgcg caccgtcacg accaaaacag tacaaacgca cgttgtcgcc
    51781 attcagatag cgcgccagtg gactgaagtc cgccgattta ccatcgccgc ccagcaacaa
    51841 atgcagcgtg ccgtctacct gcaagccatt caacgccgct tccgtactgc cgacgttagt
    51901 ggctttcgaa tcgttaatcc agcgtacgcc attgtgctcc agcaccactt caaagcgatg
    51961 cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg gcgcgcggca aaccagctgc
    52021 gtctgccagt gccagtgctg ccagcgcatt ggtgtagtta tgctgcccgg aaagtttcat
    52081 ctctttcaca ttcagcactt tctcgccttt aacccgcagc caggtttcgc cctgctgatg
    52141 attcaggtga tagtcaccca tgttgacgcc aaagctgacg cagcgttcat cagcaccgcg
    52201 aatcggcatt gttaaggcat catcagcatt gacgacacaa actttcgcat tttcgtaaat
    52261 acgcaatttt gccgcacggt attgctgcaa accaaacgga tagcggtcca tatgatcttc
    52321 agtcacgttc agaatggtcg ccgccaccgc ctgtaagctg gaagtggttt ccagctggaa
    52381 gctcgacagt tccagcacgt acagttcgca ctcagcatca agcagcatca acgctggcag
    52441 gccaatattg ccacccacac caacgttaac ccccgccgct ttcgccattt cacccactag
    52501 cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg cttgtgcttc
    52561 gcgacagaac agctcgatat cgccaacgat ttcgattccg gcatcagcgg cagcgcttaa
    52621 ggatggatgc gcctgtgcaa taccgggact ggcgacaatc agatctgccg ccatcagcca
    52681 ttcatcattc agactgcccg tgtggcgttc tacggcttcg ggtaatttat ccaggccagg
    52741 cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga aaaagtccac
    52801 gcaggaaagc ccggtgaggc ccaggccgat aatgacgaca tttttaccct gataatcagc
    52861 catgattaac gtaccttcag cgttgccaga ccaatcagaa ccagcatcag cgaaataatc
    52921 cagaaacgca caatgacgcg cggttccggc cagcctttca gttcatagtg gtgatgaatc
    52981 ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg caggatgaca
    53041 gaaagcgttt ctaccacgaa cacgcccccc ataatcacca gcaggaattc ctgacgtagc
    53101 agtacggcga taatgcctaa cgcaccacct aacgccagcg aacctacatc gcccataaag
    53161 acctgcgccg gataggtgtt aaaccacagg aagcccagtc ctgccccgac tatcgccgtg
    53221 cagacaatga ccagttcccc tgcgtgtcgc agatacggta tatgcaagta gctggcaaag
    53281 ttcatattgc cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc
    53341 ataattgcca ggccgtcgag accatcggtc aggtttaccg cgttgccagt acccacaatg
    53401 acgaagtaag ccagcagaat gtagaacagc cccagctgcg gcatcacatc tttaaagaat
    53461 gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa ggtacagggc gaaggcgaca
    53521 cccagcgcaa tgaccgacat ccagaaatac ttccaacgag cgatcaaccc tttggtgtct
    53581 ttacgcacca ctttgcgata atcatcaaca aagccaataa caccgtaacc taccagcacc
    53641 accaacacgc accagacgta cggattggac gggtaagccc acagcagtac ggagatcaca
    53701 atcgccgtca ggatcataat cccgcccatg gtcggcgtac cgcgcttgct gaagtgtgat
    53761 tcaggaccgt cgttacgcac cacctgacca aaggaaagtt tttgcaaatg agctatcata
    53821 cgcgggccca tccacaatga gatgaacagc gcggtcagca ggctgacgat ggcgcgaaac
    53881 gtcagatagg aaaagacgtt aaagccggaa taatatttga ccaaatgttc ggccagccaa
    53941 actaacatgt cccattctcc tgtaaagcgc gtactacctc ttccatggcg gcactacgtg
    54001 aacccttaac taaaatcgta attacctgtt gctcagcaat cagtgattta agacgcgtaa
    54061 ttaacgcagt tttatcagca aaatgttcgc caacgccgct ggcggtgctg atagcatggc
    54121 tttgtttacc cacgcttaac acgcggtcaa taccagcagc ttttgccgcc tcgcccacct
    54181 gtacatggca ggcttcgctt tcagcgccca gttccgccat atcgcccacc accagcacgc
    54241 ggtagcccgg catttcagcc agtacctgaa cagcggcggt cattgaaccg acattggcgt
    54301 tgtaggagtc gtcgagcagc aactggtttt ctgccagttg gatggggaac agacggcctg
    54361 gaacagcttt cagatttgcc agccccgctt tgatagcatc aagcgttgcg cccacggaca
    54421 tggagagcgc agcggctgcc agcgcattcg caatattgtg acgccccggc aacggcagca
    54481 gaacatcgac gctaccggtt ggggtttgta gggtaaattc cgtaccgtgc gaggtcacat
    54541 ggatattggt ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc cacactttgc
    54601 gtgagccaat tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc ataatggcga
    54661 taccgttttc cggcaggccg ctaaagattt cacctttcgc tttcgcgaca cccgcaagcg
    54721 agccaaaacc ttccagatgc gccgctgcca ggttgttgac cagcgcagct tccgggcgag
    54781 tcagactcac agtccaggct atttcgccct gatggttcgc gccaagttca ataactgcgt
    54841 aatcgtattc cggcgtcaag cgcaacaacg tcatcggtac accgatgtcg ttgttgagat
    54901 tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc atctctttaa
    54961 cggaggtttt gccggaggaa cctgtcagag caaccacgcg cgccggaact tgctggcgaa
    55021 cccatgcagc cagttcacca aacgccagac gcgtatcctt gacgattaac tgcggcaggt
    55081 cgatatccag cggacggctg accagtagtg cgcccgcgcc gccagctttc gcctggtcgg
    55141 caaaatcatg agcatcgaaa cgttcgcctt tcagggcaac aaacaggcag cccggcgtca
    55201 gttttcgggt atcagtggtt acagcatcaa gggtgatatc tgcaccttgc agttcaccgt
    55261 tgagaatgtc ggtaagttgg ctaagggtta cgctaatcat gcaatcaccc ccagcagacg
    55321 cgccaccgtg acgcgatcgg agtagtccag acgctgattg ccaacaatct ggtaatcttc
    55381 atggcctttg cccgcgacca gtaccacatc attctcttta gcctgcataa cggcgcaagt
    55441 caccgcttca gcacggcctt ccatcacttt ggcatgtccg gcatctaaca ttcccgccag
    55501 aatatcgttg atgatggcac gcggttcttc ggtacgcggg ttatcgtccg tcaccaccgc
    55561 cacgtcagca aactcttcgg caattgcgcc catcagtgga cgcttacctt tatcgcgatc
    55621 gccaccacag ccaaagacgc accacagctt gcccgcacag tgcaggcgcg ccgcctgtaa
    55681 ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg gtttgcctgg
    55741 cgcagtgaac acttccatac gtccgcaaac cggttgcaga cgcgcggcgg ttttcagcag
    55801 atcagccagt ggatagccga gtgccagcag tgtcgccagc gcgagcagca ggttgctgac
    55861 gttaaaagcg cccatcagat ggctttcaat ttcgccatcg ccccaacttg agctaaagcg
    55921 aatcgtcgca ccgctgtcgt gatagttcac gtcgatcgct tttaaccagc gtccgtgaca
    55981 gttcggatta atatgatctt ccattgatac cgcaaccgcg tccggcagtt ttgccagcca
    56041 gcggcggccc acttcatcgt cggcgttaat aatcgcctga ccgcaatgat gttcagaata
    56101 aagcagccat ttcgcagctt cgtagtgttc catatcacca tgataatcaa ggtgatcgcg
    56161 gcttaagttg gtaaagaccg acgccgcaaa tttcaatgcc gccacacggt gctgtaccag
    56221 cccgtgggag gaaacttcca ttgcgcaaaa cgtcgcgccc tgctccacca gccccgccag
    56281 ctcatgctga acatcgactg cggaaccggt agtattttct gtcgggatca ctttcccaag
    56341 cagaccgtta ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt ggctccactg
    56401 cgccagcagc tgggtagtcg tggttttgcc gttggtgccc gttacgccta cgagacgtaa
    56461 attgtcagag ggttcatggt aaaagcggcc cgccagtgca gataaacgct cattgagctg
    56521 gctgagatag atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg tcgcctcatc
    56581 tttcgctttc gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata
    56641 tcgacgcccg tccgcctgat gacctactac agctacaaag agatcgcccg ccgcagccac
    56701 acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc
    56761 aagaaggtcg cgcaaattac gatctgccac ctgtcccctc gccttgatta atcacaaatt
    56821 catttttatc gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc
    56881 ccatgatggc accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg
    56941 gatcgttgat aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg
    57001 tataagcaat atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg
    57061 ttttaatggc gatacgatag cctttaatcg ccgccttcac gccgccgccg cctggtagcg
    57121 ccacgctttc catcatatgc accacagtgc ggacaatgga tcccgggaag acacgttcac
    57181 cgggaaccgg ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga
    57241 tagttgcgta gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga
    57301 aggtggccct ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc
    57361 cgaccaaccc caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg
    57421 ctgaggacgg catcgctaac gccagcttgg aaacaccgac gttactcgac ttctgtaata
    57481 ccccggtcag ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc
    57541 gataaggaat ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca
    57601 ttaccaccat cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac
    57661 gcatcgcctc tttcggcgtg ccgctcagat tgttagggtt gtatgacggg ctgttagcca
    57721 tcgccagcac ttcaccggtg ttgacatcca ccagcacggc gctaccagat tcagccttgt
    57781 taaaggccac cgcgttgttc agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac
    57841 tcagcgccag gttgtgcgct gcctggctgt cagtagaaga aatatcttca attacgcgac
    57901 catagcggtc tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac
    57961 tcttctcaac gccctcaatc ccttgactat cgacgttagt aaagccgatg aggtgagcag
    58021 tcacttcgcc ggacggatag taacggcgag actcttcacg cagatgaatc cccggcagtt
    58081 tcagtttttt gatgtagtcc gccatgtcag ggttcacctg acgcgccaga taaataaaac
    58141 gccctttcgg gttggcgtta atgcgggctg aaagctgatc cagcggaata ttgagcgcgt
    58201 tagccagcgc cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg
    58261 ggtcagccca aatcgctttt accggcacgc tcaccgctaa cgggcgacca gaacggtcag
    58321 taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt
    58381 tcaccagcat atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca
    58441 gcgccaggag aatacagccg cataacaacg caaaacgcca actgataaag ttggcatgtt
    58501 cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt
    58561 atttttgcac tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg
    58621 tggcgatcct ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc
    58681 gccattcaat gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta
    58741 aacgggtatg gtgcgccgtg gttaccacag tcaccgccgt caaaataatg caaatgaaca
    58801 ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat
    58861 ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga
    58921 tcatgcattc gtcctctctg caatacgcag aactgaacta cgggcacgag ggttctcagc
    58981 cacctcttct tcgcccggca ttaacttgcc tagtgctcgc agctgacggc cacccagttt
    59041 tttgagctgc tcttcagtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc
    59101 acgcataaaa cgtttcacaa tacggtcttc cagcgagtgg aagctgatga tcgaaagccg
    59161 cccacccggg gccagcacgt tgagcgagct ttttagcgcc tgctctatct cctccagttc
    59221 actgtttacc caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc
    59281 tttcaccggc gttgcaacag ccacgacttc cgccagttct ttggtgcggg tcatcggctg
    59341 ttcgcggtta cgctcgacaa tggcgcgggc aatgcgtttg gcaaaacgct cttcaccata
    59401 ggttttcaat acccaagcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga
    59461 ctgcccacgg gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc
    59521 gcgctcagca tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat
    59581 cttgccgata agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat
    59641 gatggagaag cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc
    59701 gatcgccagc aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtgaccacc
    59761 gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc
    59821 ttcatccagc agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct
    59881 gcagtcgctc cgataagtct ccggtagcca actgctctgc gtcgatatct tccttgacct
    59941 gttgatgcca ggttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca
    60001 cttcttttgt cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc
    60061 catccatctg acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaaccg
    60121 ggttcatgct cgacagacgc gataattttt gctcgataat ttcccattca ggcagggggt
    60181 aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg acagcgttct
    60241 caagcagctg ttcccggtaa cgggtaggca ctgataagcg ccctttgctg tcgagattga
    60301 ctaacgttgc tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt
    60361 tcccactgaa aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata
    60421 ccgaggtaaa aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg
    60481 tgcgcagcaa attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcaccgt
    60541 tttaaaatga ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg
    60601 cattttagga catatcttcc ccggttaaca gtccctgttg cgtctgtgtg gcgcttgatg
    60661 ccagcgggtt tgcgcaaaag caaagaaaag tgcttgttaa atcggcaatt catctctgca
    60721 tgtaacaaaa taatgcaaaa acgcgctgtc gatatcactt aattattgtg aatttcagct
    60781 ggtgagttaa ttaacgggcg ggtttaaaag aacttatcaa taatagtcag gaatagtctt
    60841 atttacttta aggatattga tgtccattcc cgtactctac g
//
LOCUS       DAJTVJ010000007        59579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000007 DAJTVJ010000000
VERSION     DAJTVJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.007"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..262)
                     /locus_tag="OO448_000414"
     CDS             complement(<1..262)
                     /locus_tag="OO448_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6335727.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="OO448_000415"
     CDS             369..665
                     /locus_tag="OO448_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6335728.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OO448_000416"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OO448_000416"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6335729.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OO448_000417"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OO448_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6335730.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OO448_000418"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OO448_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6335731.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="OO448_000419"
     CDS             complement(3472..3645)
                     /locus_tag="OO448_000419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335732.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="OO448_000420"
     CDS             3673..3756
                     /locus_tag="OO448_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6335733.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OO448_000421"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OO448_000421"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6335734.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OO448_000422"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OO448_000422"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6335735.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="OO448_000423"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="OO448_000423"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6335736.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="OO448_000424"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="OO448_000424"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6335737.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OO448_000425"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OO448_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR6335738.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OO448_000426"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OO448_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6335739.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="OO448_000427"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="OO448_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6335740.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OO448_000428"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OO448_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6335741.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="OO448_000429"
     CDS             14052..15254
                     /locus_tag="OO448_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6335742.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="OO448_000430"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="OO448_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16953..18560
                     /locus_tag="OO448_000431"
     CDS             16953..18560
                     /locus_tag="OO448_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6335743.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18822..20444
                     /locus_tag="OO448_000432"
     CDS             18822..20444
                     /locus_tag="OO448_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6335744.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20810..21877
                     /locus_tag="OO448_000433"
     CDS             20810..21877
                     /locus_tag="OO448_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6335745.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21874..24609
                     /gene="rbbA"
                     /locus_tag="OO448_000434"
     CDS             21874..24609
                     /gene="rbbA"
                     /locus_tag="OO448_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6335746.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24609..25733
                     /locus_tag="OO448_000435"
     CDS             24609..25733
                     /locus_tag="OO448_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6335747.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25806..26079
                     /locus_tag="OO448_000436"
                     /pseudo
     CDS             25806..26079
                     /locus_tag="OO448_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26076..26435
                     /locus_tag="OO448_000437"
     CDS             26076..26435
                     /locus_tag="OO448_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6335748.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OO448_000438"
     CDS             complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OO448_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6335749.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OO448_000439"
     CDS             complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OO448_000439"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6335750.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OO448_000440"
     CDS             complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OO448_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6335751.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OO448_000441"
     CDS             complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OO448_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6335752.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OO448_000442"
     CDS             complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OO448_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6335753.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OO448_000443"
     CDS             complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OO448_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6335754.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OO448_000444"
     CDS             complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OO448_000444"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6335755.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32630..33679)
                     /locus_tag="OO448_000445"
     CDS             complement(32630..33679)
                     /locus_tag="OO448_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6335756.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33811..35028
                     /locus_tag="OO448_000446"
     CDS             33811..35028
                     /locus_tag="OO448_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6335757.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OO448_000447"
     CDS             complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OO448_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6335758.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35662..36327)
                     /locus_tag="OO448_000448"
     CDS             complement(35662..36327)
                     /locus_tag="OO448_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6335759.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36548..36793
                     /gene="tusA"
                     /locus_tag="OO448_000449"
     CDS             36548..36793
                     /gene="tusA"
                     /locus_tag="OO448_000449"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6335760.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OO448_000450"
     CDS             complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OO448_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6335761.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39167..39793)
                     /locus_tag="OO448_000451"
     CDS             complement(39167..39793)
                     /locus_tag="OO448_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6335762.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39934..40293
                     /locus_tag="OO448_000452"
     CDS             39934..40293
                     /locus_tag="OO448_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6335763.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40296..40565)
                     /locus_tag="OO448_000453"
     CDS             complement(40296..40565)
                     /locus_tag="OO448_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6335764.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OO448_000454"
     CDS             complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OO448_000454"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6335765.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41301..42797
                     /gene="ftsY"
                     /locus_tag="OO448_000455"
     CDS             41301..42797
                     /gene="ftsY"
                     /locus_tag="OO448_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6335766.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42800..43468
                     /gene="ftsE"
                     /locus_tag="OO448_000456"
     CDS             42800..43468
                     /gene="ftsE"
                     /locus_tag="OO448_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6335767.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43461..44519
                     /gene="ftsX"
                     /locus_tag="OO448_000457"
     CDS             43461..44519
                     /gene="ftsX"
                     /locus_tag="OO448_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6335768.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44764..45618
                     /gene="rpoH"
                     /locus_tag="OO448_000458"
     CDS             44764..45618
                     /gene="rpoH"
                     /locus_tag="OO448_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6335769.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45889..46992
                     /gene="livJ"
                     /locus_tag="OO448_000459"
     CDS             45889..46992
                     /gene="livJ"
                     /locus_tag="OO448_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6335770.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OO448_000460"
     CDS             complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OO448_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6335771.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47998..49107
                     /gene="livK"
                     /locus_tag="OO448_000461"
     CDS             47998..49107
                     /gene="livK"
                     /locus_tag="OO448_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6335772.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49155..50081
                     /gene="livH"
                     /locus_tag="OO448_000462"
     CDS             49155..50081
                     /gene="livH"
                     /locus_tag="OO448_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6335773.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50078..51355
                     /gene="livM"
                     /locus_tag="OO448_000463"
     CDS             50078..51355
                     /gene="livM"
                     /locus_tag="OO448_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6335774.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51352..52119
                     /gene="livG"
                     /locus_tag="OO448_000464"
     CDS             51352..52119
                     /gene="livG"
                     /locus_tag="OO448_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6335775.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52121..52834
                     /gene="livF"
                     /locus_tag="OO448_000465"
     CDS             52121..52834
                     /gene="livF"
                     /locus_tag="OO448_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6335776.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53118..54182
                     /gene="ugpB"
                     /locus_tag="OO448_000466"
                     /pseudo
     CDS             53118..54182
                     /gene="ugpB"
                     /locus_tag="OO448_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54280..55167
                     /gene="ugpA"
                     /locus_tag="OO448_000467"
     CDS             54280..55167
                     /gene="ugpA"
                     /locus_tag="OO448_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6335777.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55164..56009
                     /gene="ugpE"
                     /locus_tag="OO448_000468"
     CDS             55164..56009
                     /gene="ugpE"
                     /locus_tag="OO448_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6335778.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56011..57081
                     /gene="ugpC"
                     /locus_tag="OO448_000469"
     CDS             56011..57081
                     /gene="ugpC"
                     /locus_tag="OO448_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR6335779.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            57078..57821
                     /gene="ugpQ"
                     /locus_tag="OO448_000470"
     CDS             57078..57821
                     /gene="ugpQ"
                     /locus_tag="OO448_000470"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6335780.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57808..58248)
                     /locus_tag="OO448_000471"
     CDS             complement(57808..58248)
                     /locus_tag="OO448_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6335781.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58368..>59579
                     /gene="ggt"
                     /locus_tag="OO448_000472"
     CDS             58368..>59579
                     /gene="ggt"
                     /locus_tag="OO448_000472"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6335782.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAV"
BASE COUNT        14537 a        15046 c        16060 g        13936 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata
    16741 tttatcctaa gattaattct tattctgttg ttttttttac aattatcttc cagggatgga
    16801 ttgttttcca ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata
    16861 taagtattat tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg
    16921 ttactaacaa gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc
    16981 acttctcttc aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg
    17041 gcaaggttga taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt
    17101 cgcaaataga taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga
    17161 gggtaaatga acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta
    17221 tagatattca agaatataat agtttcgccg ataatactac taacacgttt atctttacaa
    17281 ttataccaga caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt
    17341 gtaaggatgg ttattatttt attaatttct ccggagataa atcagacata atttataaat
    17401 cagacataac ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc
    17461 ttagcggtaa ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg
    17521 ataagtttga tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg
    17581 gtgtgattaa cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag
    17641 gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga
    17701 gtttattatg tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa
    17761 ataataaatt gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc
    17821 aacatgtttt tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag
    17881 gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc
    17941 gaaatatgga tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa
    18001 atcatgaaat caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg
    18061 ccggtgatac gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga
    18121 aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg
    18181 gtgctttgca ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa
    18241 atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa
    18301 gcaatgctgt agcatccaca cctgtatatg aaaattattt taataacact actgctttca
    18361 gaccaaaacc tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat
    18421 atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc
    18481 taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta
    18541 gttgttttca acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca
    18601 gtatttcata ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc
    18661 tttagggact ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga
    18721 tttgtgcaaa atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg
    18781 ttaactatgt attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg
    18841 caggcactaa cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta
    18901 ccaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa
    18961 gtattgccag gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac
    19021 ggttccaaca atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag
    19081 aaaaatatag tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc
    19141 agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta
    19201 attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg
    19261 ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat
    19321 ttgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca
    19381 attgttcacc tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct
    19441 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag
    19501 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
    19561 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
    19621 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
    19681 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
    19741 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
    19801 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
    19861 cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca
    19921 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
    19981 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
    20041 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
    20101 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
    20161 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
    20221 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
    20281 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
    20341 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
    20401 gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
    20461 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
    20521 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
    20581 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
    20641 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
    20701 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
    20761 gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag
    20821 taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg
    20881 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
    20941 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
    21001 agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca
    21061 ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc
    21121 gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca
    21181 acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca
    21241 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
    21301 agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc
    21361 ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg
    21421 tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta
    21481 tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
    21541 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
    21601 tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
    21661 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
    21721 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
    21781 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc
    21841 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
    21901 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac
    21961 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
    22021 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
    22081 atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc
    22141 gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac
    22201 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
    22261 gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
    22321 ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg
    22381 atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg
    22441 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
    22501 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
    22561 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
    22621 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
    22681 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
    22741 gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt
    22801 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
    22861 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
    22921 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
    22981 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
    23041 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
    23101 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
    23161 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
    23221 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac
    23281 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
    23341 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
    23401 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
    23461 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
    23521 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
    23581 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
    23641 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
    23701 cgttacttta tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt
    23761 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
    23821 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
    23881 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
    23941 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg
    24001 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
    24061 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac
    24121 ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc
    24181 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
    24241 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
    24301 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
    24361 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
    24421 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
    24481 atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
    24541 ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag
    24601 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
    24661 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
    24721 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
    24781 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
    24841 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
    24901 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
    24961 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
    25021 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
    25081 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
    25141 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
    25201 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
    25261 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
    25321 tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
    25381 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
    25441 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
    25501 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
    25561 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
    25621 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
    25681 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
    25741 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
    25801 atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata
    25861 tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca
    25921 ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc
    25981 gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc
    26041 gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact
    26101 ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc
    26161 gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc
    26221 tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta
    26281 ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc
    26341 tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
    26401 ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga
    26461 atatctgcct ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag
    26521 ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca
    26581 aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc
    26641 ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca
    26701 gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt
    26761 cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct
    26821 gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt
    26881 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
    26941 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
    27001 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc
    27061 caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg
    27121 ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
    27181 ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc
    27241 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
    27301 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
    27361 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
    27421 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
    27481 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
    27541 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
    27601 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
    27661 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
    27721 accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc
    27781 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg
    27841 ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg
    27901 tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct
    27961 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
    28021 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
    28081 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
    28141 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
    28201 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg
    28261 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc
    28321 gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
    28381 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
    28441 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
    28501 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat
    28561 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
    28621 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
    28681 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
    28741 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
    28801 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
    28861 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc
    28921 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
    28981 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
    29041 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
    29101 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
    29161 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
    29221 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
    29281 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
    29341 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
    29401 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
    29461 cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
    29521 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
    29581 gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc
    29641 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac
    29701 gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga
    29761 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
    29821 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
    29881 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
    29941 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
    30001 cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat
    30061 accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg
    30121 atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
    30181 taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
    30241 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
    30301 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
    30361 gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga
    30421 taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt
    30481 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat
    30541 ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt
    30601 ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
    30661 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
    30721 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac
    30781 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
    30841 caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg
    30901 ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca
    30961 tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
    31021 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
    31081 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
    31141 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
    31201 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
    31261 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
    31321 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
    31381 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
    31441 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
    31501 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
    31561 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
    31621 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
    31681 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
    31741 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca
    31801 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
    31861 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
    31921 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
    31981 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg
    32041 tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg
    32101 agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
    32161 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
    32221 cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg
    32281 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag
    32341 cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag
    32401 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
    32461 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
    32521 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
    32581 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
    32641 accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg
    32701 tttccatcca gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc
    32761 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    32821 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    32881 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    32941 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
    33001 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
    33061 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
    33121 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    33181 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    33241 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    33301 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    33361 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    33421 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    33481 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga
    33541 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    33601 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    33661 cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    33721 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    33781 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    33841 aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac
    33901 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    33961 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    34021 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    34081 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    34141 gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt
    34201 gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga
    34261 gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc
    34321 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
    34381 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca
    34441 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct
    34501 tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
    34561 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    34621 ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt
    34681 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc
    34741 gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta
    34801 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
    34861 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg
    34921 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    34981 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    35041 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    35101 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    35161 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    35221 ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
    35281 cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    35341 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    35401 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    35461 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    35521 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    35581 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    35641 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    35701 cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    35761 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    35821 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    35881 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    35941 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    36001 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    36061 tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg
    36121 acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg
    36181 gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    36241 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    36301 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    36361 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    36421 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    36481 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    36541 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    36601 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac
    36661 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    36721 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    36781 taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc
    36841 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    36901 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    36961 caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    37021 ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    37081 catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc
    37141 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    37201 gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt
    37261 gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc
    37321 cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag
    37381 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    37441 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    37501 caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    37561 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat
    37621 ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc
    37681 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    37741 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    37801 agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    37861 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    37921 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag
    37981 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg
    38041 gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag
    38101 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    38161 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat
    38221 ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    38281 ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat
    38341 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    38401 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    38461 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    38521 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    38581 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    38641 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat
    38701 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    38761 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc
    38821 atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg
    38881 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca
    38941 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc
    39001 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    39061 ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca
    39121 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg
    39181 agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    39241 ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag
    39301 cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    39361 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    39421 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    39481 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    39541 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    39601 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    39661 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    39721 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    39781 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    39841 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    39901 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    39961 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    40021 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    40081 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    40141 atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    40201 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    40261 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    40321 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc
    40381 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    40441 agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc
    40501 accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc
    40561 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    40621 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    40681 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    40741 ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    40801 tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    40861 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    40921 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    40981 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    41041 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    41101 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    41161 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    41221 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    41281 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    41341 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    41401 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag
    41461 agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    41521 tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc
    41581 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    41641 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    41701 ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    41761 agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc
    41821 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag
    41881 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    41941 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    42001 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    42061 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    42121 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    42181 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    42241 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    42301 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    42361 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    42421 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    42481 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    42541 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    42601 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    42661 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    42721 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    42781 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    42841 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    42901 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    42961 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    43021 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    43081 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    43141 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    43201 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    43261 taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga
    43321 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    43381 gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca
    43441 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    43501 gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    43561 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    43621 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    43681 cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    43741 tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    43801 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    43861 agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    43921 actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    43981 cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc
    44041 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    44101 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    44161 cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact
    44221 ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt
    44281 atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc
    44341 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    44401 atatcaatga tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    44461 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    44521 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg
    44581 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    44641 attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct
    44701 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    44761 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    44821 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    44881 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    44941 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    45001 caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg
    45061 cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt
    45121 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    45181 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    45241 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    45301 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    45361 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    45421 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    45481 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    45541 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    45601 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    45661 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    45721 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    45781 tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac
    45841 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    45901 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    45961 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa
    46021 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    46081 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    46141 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    46201 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    46261 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    46321 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    46381 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    46441 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    46501 ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt
    46561 taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    46621 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    46681 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt
    46741 gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    46801 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    46861 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa
    46921 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca
    46981 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    47041 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat
    47101 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    47161 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    47221 cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc
    47281 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    47341 ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt
    47401 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    47461 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    47521 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    47581 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    47641 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    47701 attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt
    47761 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    47821 aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat
    47881 actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    47941 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    48001 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    48061 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    48121 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    48181 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    48241 gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt
    48301 tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg
    48361 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    48421 gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc
    48481 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    48541 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    48601 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac
    48661 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    48721 aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc
    48781 gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    48841 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    48901 atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag
    48961 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    49021 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    49081 gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt
    49141 agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg
    49201 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    49261 tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta
    49321 tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    49381 gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct
    49441 atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca
    49501 tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc
    49561 tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca
    49621 tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc
    49681 gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc
    49741 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    49801 cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct
    49861 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    49921 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    49981 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    50041 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    50101 ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact
    50161 ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt
    50221 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg
    50281 gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa
    50341 gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc
    50401 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    50461 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    50521 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    50581 gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg
    50641 tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt
    50701 gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc
    50761 gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag
    50821 taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc
    50881 gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    50941 gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc
    51001 gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac
    51061 gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    51121 gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc
    51181 ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    51241 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat
    51301 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    51361 ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    51421 gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga
    51481 aaaaccacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta
    51541 ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    51601 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    51661 gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    51721 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg
    51781 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    51841 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    51901 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    51961 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    52021 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    52081 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    52141 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    52201 tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt
    52261 gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa
    52321 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    52381 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt
    52441 tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct
    52501 gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc
    52561 gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg
    52621 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    52681 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    52741 ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa
    52801 tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    52861 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    52921 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    52981 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    53041 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    53101 tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga
    53161 aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc
    53221 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
    53281 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
    53341 ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac
    53401 tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
    53461 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
    53521 cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag
    53581 agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt
    53641 tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg
    53701 agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
    53761 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag
    53821 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
    53881 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
    53941 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
    54001 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
    54061 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
    54121 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
    54181 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
    54241 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
    54301 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
    54361 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt
    54421 ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac
    54481 tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
    54541 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat
    54601 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
    54661 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
    54721 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg
    54781 aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg
    54841 ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct
    54901 ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc
    54961 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
    55021 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
    55081 gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat
    55141 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
    55201 ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg
    55261 cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac
    55321 atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt
    55381 tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta
    55441 ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct
    55501 tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg
    55561 tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga
    55621 tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat
    55681 tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt
    55741 ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga
    55801 atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag
    55861 ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg
    55921 cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg
    55981 tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc
    56041 aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc
    56101 gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt
    56161 gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa
    56221 atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat
    56281 atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
    56341 attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt
    56401 cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg
    56461 cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc
    56521 gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac
    56581 gttactcacg atcaggttga agcgatgacg ctcgcccagc gagtaatggt gatgaacggt
    56641 ggcgttgccg aacagattgg cacaccagtt gaagtctacg aaaagcccgc cagcctgttt
    56701 gtggcgagtt ttatcggcag cccggcgatg aacctgctga caggccgcgt gaataatgaa
    56761 ggcacgcatt tcgaactgga cggcggtatc gtgctaccgc taaacggtgg ctaccgtcag
    56821 tatgccgggc gtaaaatgac tctcggcatt cgcccggaac atatagcgct aagctcgcag
    56881 gcagaaggtg gtgtgccgct ggtgatggac acgctggaga tcctcggcgc agataacctg
    56941 gcgcacggac gctggggcga gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg
    57001 acggcaggca gcacgctgtg gctgcatttg gcggaaaatc agctgcatct ttttgatggt
    57061 gaaacaggac aacgagtatg agtaactggc cttatccccg catcgtcgct catcgtggcg
    57121 gcggtaagct ggccccggaa aacaccctgg cggcaatcga cgtcggggca aaatacggtc
    57181 ataagatgat cgaatttgac gcgaagttat cgaaagatgg cgagatcttc ctgctccatg
    57241 acgacaatct cgaacgcacc agcaacggct ggggcgtcgc gggtgaactg aactggcagg
    57301 atttactgcg cgtggatgcg ggcagttggt acagcaaagc gtttaaaggt gagccgctgc
    57361 cgttgctttc gcaggtggcg gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa
    57421 tcaaacccac cactggcact ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc
    57481 aactgtgggc cggtatgacg ccgccgctgc tgtcatcgtt tgagattgat gctttagaag
    57541 ctgcacaaca ggcggcaccg gaactgccgc gtggtttatt gcttgatgag tggcgcgacg
    57601 actggcgcga actgaccgcg cggctgggct gcgtctctat tcatctcaat cataagttac
    57661 tcgataaagc gcgagtgatg cagttgaaag acgccggatt acggattctg gtttataccg
    57721 tcaataaacc ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc atctgtaccg
    57781 atgcgattga cgtgattggt ccgaacttta cggcccaata gttttcaacg gaatgtcagg
    57841 ctgcggcgtg ctgggctgat tcagcatgtc gccgtttctc ctttccggca gcatatgctg
    57901 ctgattcaac gaactatccg gattacggtt gctgtttaac atcccgccgt tggtgttggg
    57961 cagcatttgc tgccgggcgg gatttcgctc ccccggctgt gactgcaaca cccgctgaga
    58021 attattgttt atctggtttt ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg
    58081 ctgattcagc atcccttttt gctggatttg ctgagtctgc atctgggttt gcatccgctg
    58141 ctggctgggt atctgatacc ccggctggtt agggttgttc agagtattaa tgggctgtgc
    58201 aaagccgaca aacggcagga gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc
    58261 tctgaagata tcctttaagt ttactcgctt cccgacaaaa cgatgattaa ttcagagtta
    58321 tataccaggc ttagctgggg ttgcccctta atctctggag aataacgatg ataaaaccga
    58381 cgtttttacg ccgggtggcc attgctgctc tgctctcagg aagttgtttt agcgccgccg
    58441 ccgcgcctcc tgcgccgccc gtctcgtatg gtgtggagga agatgtcttc cacccggtac
    58501 gcgcgaaaca gggaatggta gcgtctgtgg acgccactgc cactcaggtg ggggtggata
    58561 tcctcaagga gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg
    58621 taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgttaatc cgctcgaaaa
    58681 atggcaatac cacggctatc gatttccgcg aaatggcacc cgccaaagcg acccgcgata
    58741 tgttcctcga tgatcagggc aacccggaca gcaaaaaatc actcacttcg catctggctt
    58801 ccggcacacc gggtacggta gcaggtttct cgctggcgct ggataaatac ggcaccatgc
    58861 cgctgaacaa agtcgtgcag cccgcgttta aactggcacg cgatggtttt atcgttaacg
    58921 acgcgctggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca
    58981 gtaaagctat cttctggaaa gagggcgagc cgctgaaaaa gggcgacacg ctggtgcagg
    59041 cgaacctggc aaagagcctg gagatgattg ctgaaaacgg cccggacgaa ttctataaag
    59101 gcacgattgc ggaacagatc gcccaggaga tgcagaaaaa cggtggcttg atcactaaag
    59161 aagatttagc ggcctataaa gcggtcgaac gcactccgat aagcggcgat tatcgcgggt
    59221 atcaggttta ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca
    59281 atattctgga aaacttcgat atgaagaaat acggctttgg cagcgccgat gcgatgcaaa
    59341 tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg
    59401 attttgtcaa agtcccgtgg caggcactga ccaataaagc ctatgccaaa tccattgccg
    59461 atcaaattga tatcaacaaa gcgaagccgt ccagtgagat tcgccctggc aagcttgcgc
    59521 cttatgagag taatcaaact acccattact ctgtagtgga taaagacggt aacgcggtg
//
LOCUS       DAJTVJ010000008        58830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000008 DAJTVJ010000000
VERSION     DAJTVJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.008"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(85..1032)
                     /gene="dusC"
                     /locus_tag="OO448_000473"
     CDS             complement(85..1032)
                     /gene="dusC"
                     /locus_tag="OO448_000473"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6335783.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1271..1669
                     /locus_tag="OO448_000474"
     CDS             1271..1669
                     /locus_tag="OO448_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6335784.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1666..2361
                     /locus_tag="OO448_000475"
     CDS             1666..2361
                     /locus_tag="OO448_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6335785.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2491..3375
                     /gene="cdd"
                     /locus_tag="OO448_000476"
     CDS             2491..3375
                     /gene="cdd"
                     /locus_tag="OO448_000476"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6335786.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3525..4244
                     /gene="sanA"
                     /locus_tag="OO448_000477"
     CDS             3525..4244
                     /gene="sanA"
                     /locus_tag="OO448_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6335787.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4247..4486
                     /locus_tag="OO448_000478"
     CDS             4247..4486
                     /locus_tag="OO448_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6335788.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4680..5918
                     /gene="preT"
                     /locus_tag="OO448_000479"
                     /pseudo
     CDS             4680..5918
                     /gene="preT"
                     /locus_tag="OO448_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            5912..7147
                     /gene="preA"
                     /locus_tag="OO448_000480"
     CDS             5912..7147
                     /gene="preA"
                     /locus_tag="OO448_000480"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6335789.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7274..8284)
                     /gene="mglC"
                     /locus_tag="OO448_000481"
     CDS             complement(7274..8284)
                     /gene="mglC"
                     /locus_tag="OO448_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6335790.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8300..9820)
                     /gene="mglA"
                     /locus_tag="OO448_000482"
     CDS             complement(8300..9820)
                     /gene="mglA"
                     /locus_tag="OO448_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6335791.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9881..10879)
                     /gene="mglB"
                     /locus_tag="OO448_000483"
     CDS             complement(9881..10879)
                     /gene="mglB"
                     /locus_tag="OO448_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6335792.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11159..12199)
                     /gene="galS"
                     /locus_tag="OO448_000484"
                     /pseudo
     CDS             complement(11159..12199)
                     /gene="galS"
                     /locus_tag="OO448_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(12341..13498)
                     /locus_tag="OO448_000485"
     CDS             complement(12341..13498)
                     /locus_tag="OO448_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6335793.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13515..14183)
                     /gene="folE"
                     /locus_tag="OO448_000486"
     CDS             complement(13515..14183)
                     /gene="folE"
                     /locus_tag="OO448_000486"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6335794.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14441..15277
                     /gene="yeiG"
                     /locus_tag="OO448_000487"
     CDS             14441..15277
                     /gene="yeiG"
                     /locus_tag="OO448_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6335795.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15309..17300)
                     /gene="cirA"
                     /locus_tag="OO448_000488"
     CDS             complement(15309..17300)
                     /gene="cirA"
                     /locus_tag="OO448_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR6335796.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17592..19061)
                     /gene="lysP"
                     /locus_tag="OO448_000489"
     CDS             complement(17592..19061)
                     /gene="lysP"
                     /locus_tag="OO448_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6335797.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19266..20147)
                     /locus_tag="OO448_000490"
     CDS             complement(19266..20147)
                     /locus_tag="OO448_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6335798.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20246..21295
                     /locus_tag="OO448_000491"
     CDS             20246..21295
                     /locus_tag="OO448_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6335799.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21369..22226
                     /gene="nfo"
                     /locus_tag="OO448_000492"
     CDS             21369..22226
                     /gene="nfo"
                     /locus_tag="OO448_000492"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6335800.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22230..23318
                     /locus_tag="OO448_000493"
     CDS             22230..23318
                     /locus_tag="OO448_000493"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6335801.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23374..24624)
                     /locus_tag="OO448_000494"
     CDS             complement(23374..24624)
                     /locus_tag="OO448_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6335802.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24724..25665)
                     /gene="rihB"
                     /locus_tag="OO448_000495"
     CDS             complement(24724..25665)
                     /gene="rihB"
                     /locus_tag="OO448_000495"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6335803.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25795..26493
                     /gene="yeiL"
                     /locus_tag="OO448_000496"
     CDS             25795..26493
                     /gene="yeiL"
                     /locus_tag="OO448_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6335804.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26560..27810)
                     /locus_tag="OO448_000497"
     CDS             complement(26560..27810)
                     /locus_tag="OO448_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6335805.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27904..28842)
                     /gene="psuG"
                     /locus_tag="OO448_000498"
     CDS             complement(27904..28842)
                     /gene="psuG"
                     /locus_tag="OO448_000498"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6335806.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28830..29771)
                     /locus_tag="OO448_000499"
     CDS             complement(28830..29771)
                     /locus_tag="OO448_000499"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6335807.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30229..31920)
                     /gene="fruA"
                     /locus_tag="OO448_000500"
     CDS             complement(30229..31920)
                     /gene="fruA"
                     /locus_tag="OO448_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6335808.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(31937..32875)
                     /gene="fruK"
                     /locus_tag="OO448_000501"
     CDS             complement(31937..32875)
                     /gene="fruK"
                     /locus_tag="OO448_000501"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6335809.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32875..34005)
                     /gene="fruB"
                     /locus_tag="OO448_000502"
     CDS             complement(32875..34005)
                     /gene="fruB"
                     /locus_tag="OO448_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6335810.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34373..35554
                     /gene="setB"
                     /locus_tag="OO448_000503"
     CDS             34373..35554
                     /gene="setB"
                     /locus_tag="OO448_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6335811.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35551..35805)
                     /locus_tag="OO448_000504"
     CDS             complement(35551..35805)
                     /locus_tag="OO448_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6335812.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            35960..36532
                     /gene="yeiP"
                     /locus_tag="OO448_000505"
     CDS             35960..36532
                     /gene="yeiP"
                     /locus_tag="OO448_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6335813.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36755..38221
                     /locus_tag="OO448_000506"
     CDS             36755..38221
                     /locus_tag="OO448_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6335814.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38339..39325
                     /gene="yeiR"
                     /locus_tag="OO448_000507"
     CDS             38339..39325
                     /gene="yeiR"
                     /locus_tag="OO448_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6335815.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SKQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39364..40077
                     /gene="lpxT"
                     /locus_tag="OO448_000508"
     CDS             39364..40077
                     /gene="lpxT"
                     /locus_tag="OO448_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6335816.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40489..41055
                     /gene="mepS"
                     /locus_tag="OO448_000509"
     CDS             40489..41055
                     /gene="mepS"
                     /locus_tag="OO448_000509"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6335817.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41236..42792
                     /locus_tag="OO448_000510"
     CDS             41236..42792
                     /locus_tag="OO448_000510"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6335818.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42874..44688
                     /locus_tag="OO448_000511"
     CDS             42874..44688
                     /locus_tag="OO448_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6335819.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44689..45783
                     /locus_tag="OO448_000512"
     CDS             44689..45783
                     /locus_tag="OO448_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6335820.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45783..46808
                     /locus_tag="OO448_000513"
     CDS             45783..46808
                     /locus_tag="OO448_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6335821.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46810..48399
                     /gene="yejF"
                     /locus_tag="OO448_000514"
     CDS             46810..48399
                     /gene="yejF"
                     /locus_tag="OO448_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6335822.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48403..48747)
                     /locus_tag="OO448_000515"
     CDS             complement(48403..48747)
                     /locus_tag="OO448_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335823.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49080..50200)
                     /locus_tag="OO448_000516"
                     /pseudo
     CDS             complement(49080..50200)
                     /locus_tag="OO448_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50228..50923)
                     /gene="rsuA"
                     /locus_tag="OO448_000517"
     CDS             complement(50228..50923)
                     /gene="rsuA"
                     /locus_tag="OO448_000517"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6335824.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51073..52833
                     /locus_tag="OO448_000518"
     CDS             51073..52833
                     /locus_tag="OO448_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6335825.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            52958..53242
                     /gene="rplY"
                     /locus_tag="OO448_000519"
     CDS             52958..53242
                     /gene="rplY"
                     /locus_tag="OO448_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6335826.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53381..54388)
                     /gene="yejK"
                     /locus_tag="OO448_000520"
     CDS             complement(53381..54388)
                     /gene="yejK"
                     /locus_tag="OO448_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6335827.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54570..54797
                     /locus_tag="OO448_000521"
     CDS             54570..54797
                     /locus_tag="OO448_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6335828.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54817..56577
                     /gene="pbgA"
                     /locus_tag="OO448_000522"
     CDS             54817..56577
                     /gene="pbgA"
                     /locus_tag="OO448_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6335829.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56652..56728
                     /locus_tag="OO448_000523"
     tRNA            56652..56728
                     /locus_tag="OO448_000523"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56686..56688,aa:Pro,seq:ggg)
     gene            complement(56831..58830)
                     /gene="yejO"
                     /locus_tag="OO448_000524"
                     /pseudo
     CDS             complement(56831..58830)
                     /gene="yejO"
                     /locus_tag="OO448_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14492 a        15338 c        14358 g        14642 t
ORIGIN      
        1 ctcggcaatt ttgacgccac caaacaaccg tgcggcctat tgataaagag ctaacacatt
       61 gtcaaaaaac atcactatgg ttttttagag tttctcgata tcaattgcct gaatagccct
      121 tgcaatatca ggggaattat tcaacacccg aacatgctga aataattccg ttgcttcatc
      181 gtattcttta cgcaaataac tcaaccactg tttaatccgc gcaacgtgat ataacccggt
      241 atcgccctgc ttttccagac gggtatattt ttgcagcaaa gcaaccacct ccggccacgg
      301 cattcgcggt tcgttatatt ttactacccg gctcaggttg ggaatattga gcgccccgcg
      361 accaatcatc actgcgtcgc agccgctgat cgcaatgcat tgttgcgcgc tctgccagtc
      421 ccagatttcg ccgttggcaa tcaccggaat attcagccgc tggcgaatct cgccaatcgc
      481 ctgccagtca atatgctccg cgcggtaacc ctgctctttt gtccgtccat gcaccaccag
      541 ctccgtagca ccagcctgtt gaaccgcatc ggcgatttca aatttcttct caccgctgtc
      601 ccagcccaga cgcactttca cgctgacggg caaatgcgcc ggtacagctt cacgcatcgc
      661 ttttgcaccc tggtagatga gttcaggatc tttgagtaac gtcgccccgc cgccgctgcc
      721 gttaaccgtt ttcgacgggc agccgcaatt gagatccacg ccccaggaac ctaattccac
      781 cgcacgggcg gcgttctctg ccagccattg tgggaactga cctaacaact gaacgcgcac
      841 cagcgtaccg gatggtgtcc ggctggcgtt ttgtagctca gggcaaatgc gatgaaagac
      901 ttttaccggc agcagttgat ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc
      961 gttaacttcg gtcagcaatt ctcgcaccag agagtcaagc acaccctcca tcggagccag
     1021 taacacacgc atatcttcac ccggaaaaaa atgaggcgct atgttagcgc ctatggtcaa
     1081 cggattaaag gtctgcaaag agggattgct gcaaactgta atccctctct atgcttgatg
     1141 tatgcctgag ttcgttgcgc attgcgtaat gccggggagc gaaaaaataa gcgctgattt
     1201 cttaatgtga tcggtagcac gttttacgaa ttaattgtat gatgaatcca tctcatctgg
     1261 ggtgttgatt atgagtaaga cactgaacat tatctggcaa tatttacgcg ctttcgtcct
     1321 gatttatgcc tgcctgtatg caggcatttt cattgcttcc ctgctaccgg taaccattcc
     1381 gggcagcatc atcgggatgc tgatcctgtt tgtcctgctg gccttgcaaa ttcttccggc
     1441 aaaatgggtc aatccggggt gctacgtact gattcgctat atggcgctat tgtttgtgcc
     1501 gattggcgta ggcgtcatgc aatattttga tttgctccgc gcacagtttg gcccggtagt
     1561 ggtttcctgt gcagtcagta cgctggtggt ttttctggtg gtgagctgga gttcacaact
     1621 ggtacacggt gaacgtaaag tcgtaggtca gaaaggatca gaagaatgat ggcgaatatc
     1681 tggtggtcat taccgctgac gttgattgtc ttttttgccg cccgcaaact ggcggcacgg
     1741 tataagtttc ccctgcttaa cccgttgctg gtagcaatgg tggtgatcat tcctttttta
     1801 atgctgaccg gcatctctta cgacagctac tttaaaggta gcgaagtgct aaacgatctg
     1861 ctgcaaccgg cggtcgtcgc gctggcctat cctttatatg agcagctaca ccagatccgc
     1921 gcgcgctgga aatcgatcat caccatctgt tttatcggca gcgtggttgc gatggtgacg
     1981 gggacttccg tggcattgtt gatgggcgct tcaccggaaa tcgccgcgtc aatcctgccg
     2041 aaatcagtca ccacgcctat tgcaatggcg gttggcggca gtattggcgg tattccggca
     2101 atcagcgcag tttgcgtgat tttcgtcggc atcctcggcg cggtatttgg ccatacattg
     2161 cttaatgcga tgcgtattcg taccaaagct gcgcgcggtc tggcaatggg gacagcctcg
     2221 cacgccctcg gtacggcgcg ctgcgccgag ctggattatc aggaaggtgc atttagttcg
     2281 ctggcgctgg tgttatgcgg gataattact tcgctgatcg caccgttcct tttcccgatt
     2341 attctggcag taatgggcta aaatttgcga tgcgtcgcgc atttttgatg taagtttcac
     2401 gcgttgcata attaatgaga ttcagatcac atataaagcc ataacgggtt cgtaaactgt
     2461 tatcccatta catgattatg aggcaacgcc atgcatccac gttttcaaac cgcttttgcc
     2521 caacttgcgg ataacttgca atctgcactg gaacctattc tggcagacaa gtacttcccc
     2581 gctttgttga ccggggagca agtctcatcg ctgaagagcg caacggggct ggacgaagac
     2641 gcgctggcat tcgcactact tccactggcg gcggcctgtg cgcgtacgcc attgtcgaat
     2701 tttaatgttg gcgcaattgc gcgcggtgtg agcggaacct ggtatttcgg tgccaatatg
     2761 gaatttattg gtgcaacaat gcagcaaacc gttcatgccg aacaaagcgc gatcagccac
     2821 gcctggttga gtggtgaaaa agcgcttgca gccatcaccg ttaactatac gccttgtggt
     2881 cactgccgtc agtttatgaa tgaactgaac agcggtctgg atctgcgtat tcatctgccg
     2941 ggccgcgagg cacacgcgct gcgtgactat ctgccggatg cctttgggcc gaaagatctg
     3001 gagattaaaa cgctgctgat ggacgaacag gatcacggct atgcgctgac gggtgatgcg
     3061 ctttctcagg cagcgattgc ggcggcaaac cgttcgcaca tgccttacag taagtcgcca
     3121 agcggtgtcg cgctggaatg taaagacggt cgtattttca gcggcagcta cgctgaaaac
     3181 gccgcattca acccgactct gccaccgttg cagggggcgt taattctgtt gaatctcaag
     3241 ggttatgatt acccggatat ccagcgcgcg gttctggcag aaaaagccga tgcgccgttg
     3301 attcagtggg atgccacctc cgcaacgctg aaagctctcg gctgtcacag tatcgaccgc
     3361 gtgcttctcg cttaagcctg gtgccggatg cggcgtgaac gccttgtccg gcttgccagc
     3421 cctctcctgg tgtcgaaatt cccggcaaac agtttgccgt ttcttgcgca aaaccagcgg
     3481 gtaaagtagc ctgatggaaa ttttccttag atcgagtctc ctgcatgtta aagcgcgtgt
     3541 tcctcagcct gttagtcctg atcggcttgc tgctgttgac tgtgctcggc ctcgatcgct
     3601 ggatgagctg gaaaaccgcg ccttatatct acgacgaatt gcaggatctc ccctaccgcc
     3661 aggtcggcgt ggtgctcggc acagcaaaat attatcgtac tggcgtaatt aatcagtatt
     3721 atcgctaccg cattcaagga gcgattaatg cctataacag cggtaaggta aattatctat
     3781 tactgagcgg cgataacgca ttgcaaagtt ataatgagcc gatgaccatg cgcaaagatt
     3841 taatcgctgc tggtgtcgac ccatcagata ttgttctcga ttacgcaggc tttcgtacgc
     3901 tggattccat cgtgcgtaca cgcaaagtgt tcgatactaa tgatttcatt attatcaccc
     3961 aacgtttcca ctgtgagcgg gcattattta ttgcgctgca tatggggatt caggctcagt
     4021 gttatgccgt accgtcaccg aaagatatgc tgtcagtacg tattcgcgaa tttgccgccc
     4081 gtttcggtgc gctggctgac ctttatattt ttagacgtga accgcgtttt ttagggccgc
     4141 tggtccctat tccggctatg caccaggtgc cggaagatgc gcaggggtat cccgccgtca
     4201 cacccgaaca gttacttgaa ttacaaaaga aacaaggaaa gtagttatgg atgtacagca
     4261 gttttttgtc gttgccgttt ttttccttat cccgatattt tgtttccgcg aagcatggaa
     4321 aggctggcgc gcaggcgcga ttgataaacg ggttaaaaat gcaccggaac cggtgtatgt
     4381 ctggcgagca aaaaatcccg gactcttttt cgcttatatg gtggcatata tcggcttcgg
     4441 aattttatct atcggcatga ttgtttatct tattttctat cgttaatatt ttctcttttc
     4501 tgttaattat tgagaataat tattacttca ctcaataagc tgcggatata acattctgaa
     4561 ccgcagctat ttgtgaatcc tttcacagtt taaatttccc cacacgctta gccttaatat
     4621 cagcacgcaa tatttattta gcgttcgcct taattactca tagcattaag gaagatcaca
     4681 tgccgcaaca aaattatctg gatgaactca ctccggcttt tacgccttta ctggcgatta
     4741 aagaagcctc tcgctgttta ttatgtcatg acgctccctg tagtcaggct tgcccggcac
     4801 agaccgatcc ggggaaattt attcgctcaa tctactttcg taattttaaa ggcgctgccg
     4861 agacaattcg cgaaaataat gccctcggtg ccgtttgtgc cagagtgtgc ccgacggaga
     4921 aattatgcca aagcggttgt acccgtgccg gtgttgatgc tcccattgat atcggccgct
     4981 tacagcgttt tgttactgat tttgaacaac aaaccgggat ggaaatttat cagcccggta
     5041 ctaaaacgct cggcaaagtc gcgattattg gcgcaggtcc tgccggattg caggccagtg
     5101 tgacactgac aaaccagggt tatgacgtca cgatctatga gaaagaagca caacccggtg
     5161 gctgattgcg taacggcatt ccgcaattcc ggttaccgca atcagtactg gatgcagaga
     5221 tcgcccgtat tgaaaaaatg ggcgtgacca ttaagtgcaa caacgaagtc ggtaacacac
     5281 tcacccttga gcagctgaaa gcagaaaacc gcgcggtact ggtcaccgtg gggttatcaa
     5341 gcggttccgg gctaccgctg tttgagcata gtgacgttga gattgccgtc gacttcttgc
     5401 aacgtgcacg acaggcgcaa ggcgatatca gcattccaca aagcgcatta attatcggcg
     5461 gcggtgatgt cgcgatggac gtagccagca cgctgaaagt tctcggctgt caggcggtaa
     5521 cttgcgtagc gcgtgaagag ttagatgagt ttccggcaag cgaaaaagag tttaccagcg
     5581 cccgggagct gggcgtttcg atcatcgatg gattcacgcc agtagccgtg gaaggcaata
     5641 aagtcacgtt taagcatgta cggttatcgg gcgaactgac gatggcggca gataaaatta
     5701 ttctcgccgt cggtcagcat gccagactag atgcctttgc ggagttagag ccgcagcgta
     5761 acaccatcaa aacacaaaat taccagaccc gcgacccgca agtctttgct gctggcgata
     5821 ttgttgaggg agacaaaacc gtggtctatg ccgtgaaaac cgggaaagaa gccgccgagg
     5881 cgattcatca ctatttagag ggagcttgct catgttaacg aaagatcttt cgattacttt
     5941 ttgcggcgtg aagtttccca acccgttctg cctctcttct tcgccggtag gcaactgcta
     6001 tgagatgtgt gccaaagcct acgacacagg ttggggcggt gtggtgttta aaacgatcgg
     6061 cttttttatc gccaacgaag tctcgccgcg ttttgatcat ctggtgaaag aagataccgg
     6121 ttttatcggc ttcaaaaata tggagcagat tgccgagcat ccgctggagg agaatctggc
     6181 cgccctgcgt cggctgaaag aagattaccc ggacaaagta ttgatcgctt cgatcatggg
     6241 ggaaaatgag cagcaatggg aggagctggc gcgcctggtg caagaagctg gcgcggatat
     6301 gatcgagtgt aacttctcct gtccgcaaat gacttctcat gcgatgggta gcgatgtcgg
     6361 gcaaagcccg gagctggtag aaaaatattg tcgggcagtg aaacggggtt ccacgctgcc
     6421 aatgctggcg aagatgacgc cgaatatcgg tgatatgtgc gaagtggcgc tggcggcgaa
     6481 gcgcggcggc gcagatggca ttgcggcgat taacaccgtt aaatccatca ccaatatcga
     6541 tcttaatcag aaaatcggta tgccgatcgt taacggaaaa tcaagtattt ccggatattc
     6601 cggtaaagcg gtaaaaccga tcgccctgcg cttcattcag caaatgcgca cccatccaga
     6661 actgcgcgat ttcccaatca gcggtatcgg cggcattgaa acctgggagg atgcggcgga
     6721 gtttttattg ctcggcgcag caacgttaca ggtcaccacc ggcattatgc agtacggcta
     6781 tcgcatcgta gaagatatgg cgagcgggtt atcgcattat ctcgccgatc agggatttga
     6841 ttcgttgcag gagatggtag gtctggcgaa taacaatatt gtcccggcgg aagatttaga
     6901 ccgcagttat attgtctatc cccgtatcaa tcttgacaaa tgtgttggct gtggacgctg
     6961 ttatatttcc tgttacgacg gcggtcacca ggcgatggaa tggagcgaga aaacccgcac
     7021 accgcattgt aataccgaga aatgtgtggg ttgtctgctt tgtggtcacg tctgcccggt
     7081 gggttgtatt gagctcgggg aagtgaagtt taagaaaggc gagaaagaac acccggtaac
     7141 gttgtagaac ccgcgtcaca tccggcattt ggttcctgtt gccggatgcg gcgtgaacgc
     7201 cttatccggc ctacaaaacc acacagatca tgatgttatg tgcgtttatg accgaatgcg
     7261 gaccacattc acatcatttc ttacgcgcgt atttcagtga atccagcgct acggcgaaga
     7321 taataatcgc ccctttgatg atgtactgcc agtatgggtt tacgccgata tacgtcaggc
     7381 catagttgat gacggtaaaa ataatcaccc cggtcaccac gccaatcacc gtccccacac
     7441 cgccgctgaa cgatacaccg cctaccacgc acgccgcgat agcatccagc tcatacataa
     7501 agccgaggtt gttggtggca gagccgatac gtccggcttc taacatcccg ccaaaggcat
     7561 agaacacgcc agacaacgcg tagatcatca gcaggttcag gccgacgttg acaccagata
     7621 cttttgccgc ttccgggtta ccgccaatgg caaaaatgtt cttaccgaag cgggttttgt
     7681 tccacaacac ccagacgaac gccaccgcaa tcaacgcgta gaaggtgatg taagagagac
     7741 ggaaactccc cagcgcgaca aagccctgag caaaggtaga gaagccactg tcaaaaccag
     7801 aaattggcga cgccccgaca aagtcgtaat agagcgagtt gatgccatag acgatgatca
     7861 tcgtgccgag cgtggtaatg aacggcgtca cgttgagata agcgataatc agaccgttga
     7921 tcaaaccgat caccgcacca atggcacaga caatcagaat aaccagcgca atcggcatcg
     7981 tcgccatttc cgggaacact ttgttggcgt tatccatgga ctgcaataat gtcgcagcca
     8041 ccactgccgc cagccctacc tgacgaccag cagaaagatc ggtcccctgg gtgacaatta
     8101 accctgccac accgagcgcg ataataatac gcaccgatga ctgggtgaga atattactta
     8161 agttcaacag acttaaaaat gttgggtcct ggaaaataat aatcgccagc aaaactaaaa
     8221 gaacgacgta aataccgccc tctttcaggt aagtaagaaa acttttctta tttaacgcac
     8281 tcatgggaag cccctaatct taaaggtgca aagacgcaag acgcagaatt tcgttttgcg
     8341 ttgttgtttt tgtatcgaca attccggaaa cgagaccatt gctcatgacc agaatacggt
     8401 ctgttatccc taacaactca ggcatttcag aggagataat aataatcccc ttgcctttct
     8461 tcgccagttc ggcaattaac tgataaattt caaacttcgc cccgacatca ataccgcgcg
     8521 tcggttcatc gagcattaat atttctggtt gcgttagtag ccagcgacca ataatcacct
     8581 tttgctgatt accaccggag agcgaaccaa tttgcgtccg atgacccggc gtttttaccc
     8641 gcatcgaatc aatcacccac tgggtatcgc ttttcatccg cgagttatcc agtaaaccaa
     8701 ctttattttt gtagttgcga atattggaaa ttaacgagtt aaaaccaata tccagatagg
     8761 cataaattcc cgttgagcgg cgctcctcag ttaccagtgc aaatccgtgg tttatggctt
     8821 cgttggcatt atggttattg atctgtttgc cgtgcaacgt aatggtgcca gccgatttct
     8881 cgcgaatacc aaataacgtc tcaacaatat cggtacgttt cgcccccacc agcccggcaa
     8941 taccgaggat ctccccttta tgcagatcaa acgagacatc gcgaatcgac ggctggcgca
     9001 gtgacgttag gttacgcacc tcgaggatga cttcgcccgg cttgttttct ttgtcaggaa
     9061 aacgctgatt aagagaacgc ccaaccatca tggcgatgat cttgtccatc gtcagtcctg
     9121 ccagcggttc ggtggcgatc cactgaccgt cgcgcaatac ggtaacttca tcacataact
     9181 ggaagatttc ttccatttta tgcgagatat aaacaatacc gcagccgcgc tcttttaatt
     9241 tacgaataat agtgaacaga tgattgacct ctttttcggt taacgaagaa gtcggttcat
     9301 ccataatcac aattttcgcg ttataggaaa acgctttggc gatttcgatc atctgcattt
     9361 gcgaaacgga taatgtgccg acgcgcgcac gcggatcgat atcaatatcc agttcatcaa
     9421 aaattgcttt ggtttcgcgg tacattttgt cctgatcgac aaacatgcct ttggtgggat
     9481 atcgccccag ccacatgtta tccatcaccg aacgttgtaa taccaggttt aactcctggt
     9541 gtaccatcga aataccattt tccagtgcct ctttggcgga atggaaatcg atctctttac
     9601 cctggaataa aatggtgccg gagtcttttt gataaatacc aaacaggcat tttaataatg
     9661 tcgattttcc tgcaccgttt tcccccatta atgcatggat agaatgtggc cggactttta
     9721 aattaacgtt atcaagtgcc ttaacaccag gaaaggactt gttgataccg ctcatttcca
     9781 acaagtattc cccggaagac ggagtcgttg agctgaccat ataattttac cttgttggcc
     9841 atacaataag ggcgcagtaa acgactgcgc ccaatcagtc ttatttcttg ctgaattcag
     9901 ccaggttgtc tttatctacg ccaacataag gtacgcggac cactttgttg tcgattttcc
     9961 agttggtgcc atcagccgca cctttgccat cggccaggtt tttcgccaga tcaaaggtcg
    10021 ctttcgcctg gttgttagca tcgttcagta ctgtgcccgc cagtgcaccg gatttcacca
    10081 gcgccagcgc ttctggcagc gcatcgacgc caaacaccgg aatgctggac ttgttgtgtg
    10141 ctttcagtgc ttctaccgcg cccattgcca tcgcatcgtt gttggcgata accacttcga
    10201 ttttgttggc gttcgggcca gacagccagg cgtccatctt atctttcgcc tgagcggtgt
    10261 cccacattgc ggtatctaac tgtaactgtt cagttttgat gcctttatcg ttcaattctt
    10321 taatcacgta agtggtacgt gcttctgcat ccggatggcc cggttcacct ttcagcagta
    10381 cgaactgaat ctgaccgtct ttgttcagat cccaaccctg attcgccgcc cagtgtttag
    10441 caatcaaatc gccctgaata atgccggact ctttggagtc agtgccaacg tagtaggctt
    10501 tgtcgtagct atccagcgcc ttacgagacg gttctttgtt gaagaaaacc accggcacgt
    10561 tttgcccacg cgctttctca atcaccgtac ccgcagctgc cgggtcaacc aggttgattg
    10621 ccagtgcctt cacccctttc gccagcaata cgtcgatctg atcgttctgc ttggactggt
    10681 cattctgaga atcattcatc agcagctgaa catctggcgc ggctttcgca tcttgctcaa
    10741 tagccttgcg cactacagac ataaagttat cgtcgtactt atagattgtt acaccaatgc
    10801 gagtatcagc agcgtgtgca gcggcaccga ataacatgct ggccatcaca gcagacaggg
    10861 ttaacacctt cttattcatg gtatctccgg tttttcttat gcagggtagt gcttgagata
    10921 aatgctcggc ggggcagtag agttaatgaa gtgttactga acgccgaagc tcactttttt
    10981 aaattcgttc ttccatgccc ggtaacgctc cagaaaacgg ctttaattgt tgttatgacg
    11041 ctattacctc ggtgaaagtg attattcact gttacatacg ggttacaacg ttaaaacggt
    11101 gcaatcatag ctatcacatt gttaagatac tgtgaaatca ctcacagatt gaaagcggtt
    11161 acatcgcctg atttgttgag ttagtgatcg ccgccgcatt ctggcgcgtt gcgacagaat
    11221 ggcgacgcac taacgtcggc ataaaacagt ggctggcacg aggatcaata ttgcctgctg
    11281 ccccctgcaa ggccagttcg gtggctaatt tagccattga agcaatggga taacgcacgg
    11341 tcgttaattg cgggtcggtg taacgggcga tgggaatatc atcgaaaccg atgattgaga
    11401 gatgtaacgg aatcgcaatg ccattatctt ttaatgctgt cagtgcgcca gcggccatat
    11461 tgtcgttata agcaaatata gcggtaagtt gtagattgcg ccccagcagt tcaaccatcg
    11521 ccgcctcacc gcccggcatg tccggcgtac cagtgccaat ccagctttcc ggcggaataa
    11581 tatcctgctc tttcaacgca ctcatccagc ctgctttacg catggcgtca tcttcaatgc
    11641 cgtgactgga agaaagataa ccaatacgtt gatgaccgtt attcagcaac atgcgcgtcg
    11701 ccattcgggc accgctgaga ttatccaggc aaacgcaacg atgggcgtac cccggcacaa
    11761 cgcggttgat taacaccata ccgggaatgt tatccataaa ttgcgccagt tcatcgtcac
    11821 tcaatgcttt tgagtgaaca atcaacgcat tacaacgctg gcgaattaac acctcaatgg
    11881 cgtgacgctc tttttccgct tcatgatagc tattgccgat tagcacgtat ttctgatgct
    11941 gctgagcgac tagatccacc gcttttacca gcgcgccgaa aaacgcatca gaaacgtcca
    12001 tcaccaccac gccaatggtg tcgctaacct gagttgccag cgcctgggca ttggcgtttg
    12061 gccgataatc cagctcactc acggctttca ttactgcttc acgcgtgtcg gcactgacca
    12121 gcgtgctgtt attgagcacc cgggaaaccg ttgccacaga gacgccagcc taacgcgcta
    12181 catcacgaat ggtgatcata ttcactacct gttatgagat tgcagtaaat gactgcttgc
    12241 tggcggctat tttgtcagca ctgaatacag gacttcgtga atcgagtcac agcaatggaa
    12301 acggttacag ccgttttgtt aacgaatgtg atccagatcg ttatcttgat gttttagata
    12361 ttgccgttcc ggcagcacgc aaagttaact gacgccagag ccattccacc ggcccctggc
    12421 ggaagtaacg cagccagata acagagaaga ggatattcgc cagccatacc ggaataacaa
    12481 acgccagcag ctccaggcgg tcaaaatgca taaacaaacc gaggtggtaa aaaagcatgg
    12541 tacaaatcag cgtttgcaat agatagttgg tcagcgccat ccgtccgacg caggcgatcg
    12601 caagcaccag cttaaagcgg ctcaattgcg gccagaagcc ataaaacagc gacgcatagc
    12661 caatcgcctg aaacggcgca ctcagttccc gcggcatttg aagtaagaag gcacaccagc
    12721 gatacgccca gtccagctgc cattgcaggg cgatggcagg aaggttaatg gtcaccccaa
    12781 tcgccaccag cacaaaacca gtacgacgat agtgacgtaa gctgaactgc cctttcagcc
    12841 agccgctgcg catcagcgcc gcaccaatga gcatcatccc tgccagttgc cagccatact
    12901 gtgcgcccag tgccagtaaa ctgttgccaa caccatcggc acgattactg atcgcttcaa
    12961 cgccgccgtg aagcttccag tatttttcat acaaaatagc cgatgcatcc ggcgtccagg
    13021 tgcggctggt ctggctgtcg gaaatcagcc ccaataacag caaaacgcca agccccacca
    13081 gataaagcat gacgcctgta ttaaacaggc tttttaccga tggcgcatcg cgcaccagcc
    13141 gccagcagat taagcccacc agcccgtaag ccagcagaat atcgccgtcc cagaacaata
    13201 aaccgtgaat aaaacccagc aagactaaca gcgttaaccg cgactggatc cagcgtctgc
    13261 cacggggcag caacatttgc aggcccgcac caaacagcag cgcaaaaagc gtgaggaatt
    13321 tcacctggcc gatgagatcg agaaatgccc aggtccatgc atcctgcggc gtaatagcgc
    13381 cgtaccaggc gggattgaga taagccgcct ttggtagccc aaaggcgctg atgtttaata
    13441 gcaggatccc cagaatggcg acgccgcgaa caaaatcgag cgttacgttg cgctccatgg
    13501 ttcctgcctt ttaatcagtt gtgatgacgc acagcgcgca gaaactcgtg gcgcgtattc
    13561 tgactggatt tgaacaatcc accaagagag gtcgttgttg tggcactggt tgcatcgcgg
    13621 atgccacgcg ctttcacgca gtaatgcacc gcatcaatgg agacagccac gttattggtg
    13681 cccagcagcg tttgtagcgc aataagaatt tgctgcgtca gacgttcctg cacctgcgga
    13741 cgctgggcaa agaactgcac gatacggtta atttttgaca gaccgatcac cgaatctttc
    13801 gggatataag ccaccgtcgc tttgccatcg atggtaacaa aatggtgttc acaggtactg
    13861 gtcagagtga tatcgcgcac ggtgaccatt tcatcgacct tcattttgtt ttcaatgagg
    13921 gtgattttcg ggaagttggc gtaatccaga ccggagaaaa tttcatcgac atacatttta
    13981 gcgatgcgat gcggcgtttc catcaaactg tcatcagcca ggtcgagatt cagcagctgc
    14041 atgatttcgg tcatatgacc agcaataagg cttttgcgcg tttcgttatc catttcatgc
    14101 acgggcgggc gcagcggtgt ttccagtcct cgcgcaacta acgcttcatg aaccagggcc
    14161 gcttctttac tgagtgatgg catttatgat ttctcctgca ggtgtgacgc ctccgccctg
    14221 cgtgggggca aagttattaa gctgatttac agcctgatta ttgtgcgtga ggcggcgcac
    14281 ataatccagt attcacagcg ataattattg taattgccgc tgactttcat cagcagatgt
    14341 taaaacatcg ttatgcaaat acggaagtga aagttactca cagcacattg aataaacggt
    14401 atgatgaaga aattgcaaac aacacaacaa ggagccacgc atggaaatgc tcgaagagca
    14461 ccgctgtttt gaaggctggc agcaacgctg gcgacacgac tccagtacct taaactgccc
    14521 gatgacgttc agtatctttc tcccaccccc tcgtgatcac accccgccac cagtgctgta
    14581 ctggctttcc ggattaacct gcaatgacga gaacttcacc accaaggcgg gtgcccagcg
    14641 cgtggcggca gaactgggga ttgtactggt gatgccagac accagcccgc gcggcgaaaa
    14701 ggttgccaac gacgatggct acgatttagg ccagggcgca ggcttttatc ttaatgccac
    14761 gcaaccgccg tgggcgacgc attaccggat gtatgattat ctgcgcgatg aattaccggc
    14821 gctggttcag tcgcaattta atgtcagcga ccgctgcgcc attagcggtc actcaatggg
    14881 tggtcacggt gcgctgatta tggcgctgaa aaatccgggt aaatacacca gcgtttcggc
    14941 ctttgcgcca attgtgaatc cgtgcagcgt cccgtgggga atcaaagcgt ttagcaccta
    15001 tttaggtgag gacaaaaatg catggctgga atgggatagt tgcgcgctga tgtatgccag
    15061 taacgcgcag gatgcgatcc cgacgcttat cgatcagggc gataatgatc agtttcttgc
    15121 cgaccagttg caacctgcgg tattggcaga agccgcgcgc cagaaagcgt ggccgatgac
    15181 gctgcgtatt cagccgggat atgatcacag ttactacttc atcgcctctt ttatagagga
    15241 tcacctgcgc ttccatgcgc agtatttact gaagtgaaag tccgcccggt tcgccgggca
    15301 tcttctcatc agaagcgata atccactgcc ataaagtaac gacgtccgtc ttcgttatag
    15361 ctgtagtcgt cacgactgag atccttgtcg ccaaggttca gcacgcctgc gcgcagtttg
    15421 acgtctttag tcacctgcca ggccgcgccg gtattccaga tggtataacc gcccggtgtt
    15481 ttagccgtcg cgctgtcggc gcgtttctgc ccggtatagt gaccagaaac atagaatgac
    15541 cagtcttcca gcgccagcgg tttccagtcc agcgtaccgt tagcagtatg gaacggcaga
    15601 tcggatagcg gtttgttttc gccgttgctg acatcacgac catcgttgta ggtgtagttg
    15661 atcgacagtt tccattcatc gttgaacgga attttcagtt cggtttccac gccctgaata
    15721 cgagctttgt taacgttgta gtagctaaat accggtatgc gccgtccgtt agcgcccgtc
    15781 tcaaaaccaa caaagttttg gtagcccggt gcagcgttga cgtcagacgt acggctgatg
    15841 ctgatacgat ctttcacatc gttacggaaa acggtaacgc tggattcaac cccttccagc
    15901 cagccttctt cacccatgta gtaaagcccc agctcccaac tttcgctggt ttctggtttc
    15961 agatccgggc tacccacaat cttacatgcg ccacggcagg aattgctcgt ccagtcaggg
    16021 ctaagttgca acagagaagg tgctttaaat gccgtcgccc agcccccttt caccgttacg
    16081 gtgtcggtgg cgttataaac caggtaggca cgcggactcc agtgttcacc gtaggtttcg
    16141 tgatcgtcca tacgcacgcc ggtcgtcagc gccagcggct cgaagatccg ccattcatct
    16201 tccacaaaca gcgcgtactg gctggcagac gttttggagc tggttccccc ggtcaggttc
    16261 accgcatcgc taagtttgtc gtgacgccat tcaccgccaa ccgtgagaaa ctgattaatc
    16321 gccgtcagcg gcaacgtgta tttgccgtcg accgtattgc tttcggaagt tatcgggctg
    16381 ctgttgccag ggtttttgtt ctcgactttc tcaccgtagt atttcagttc gctggtgccg
    16441 taatcccaac gcccattatg gctgacggag tagttctggc gttccaggcg gtttttgtcc
    16501 agcgagtcgg aatcacgatc ctgacggtcg aaaccgtatc cggcagtaaa atcgtgattt
    16561 tgattcggtg tccaggcaaa ttcgacattg ccgtcgcggc tggagaatcc ttcaatacgc
    16621 ggcgtttctc cggtatcggt ggtcgttgag ttttgcgggt catccttttc acgttttgcc
    16681 aggctgccgt aagctttcat tcccagcaca ccatcaatta atggtccact ggtaaagaac
    16741 tgaccgttat aggtatcacc gcgatcgcga tgttcctgaa tggtggtatc gacggtaacg
    16801 gtacccgacc atttctgacc gatttttttg gtgatgatat tcactacacc gccgagcgca
    16861 tcggaaccgt acagcgacga catcgggcca cggaccactt caatacgttc gatggaatcg
    16921 accgggatcc agttcagatc gaaatcattg tggcggaaga cggcattgcg ggagttcacg
    16981 cgtttaccgt cgacgagaat cagggtatag ctgctgtcca gaccacgaat actaacgccc
    17041 ttacggttat ccccttcgtt cgtcagttgt acgccaggca cttctttgag gacatccttc
    17101 agattctgta ccggttttcg ctgcagatct tcctgggtaa tgacgctgat actggcgggt
    17161 gcatctttaa ggttttgttc aacggaagat gcagtgacaa ccatcgtttc gccatcgtca
    17221 tcgaccgcta acacaggcca tacacaagaa atagcggaca aacacagccc gacccgtacg
    17281 aaagggttca acctaaacat tccatatctc catgaggtaa ctacgaaaat aaaatgggta
    17341 tcgctcacat cttctttacg tccccttgcg tacggcagca tcgcggtgga cttatttgta
    17401 tgcaggtgat catcccagaa agccggacca gcctctgatt tgtcagcctt ttttgattgt
    17461 agcgtaacga taatgcaaac gataacaatt atcaatccaa atgttaaatt ttatatccgc
    17521 atgatttgtc agggaaatgg aattgaaaaa gccctctcgg ttgagagggc ttagcaagga
    17581 agggaggaaa cttatttctt atcgttctgc gggaacttca tttcgctgta gcgtacgaag
    17641 tgagttcctt taatcagctt gtagccgaac caaataatca ggaacagcgg gataccaata
    17701 tacgttgccg ctacgccgcc ccagtcaatg gtatctttca ggaacgcttc gtagttctgg
    17761 cccaaagtga taatcagaca cagaatgaat gcgaagatcg gccccagtgg gaagaaacct
    17821 gaacggtacg gcagatcgtt aatgtcgtgt ccctgcaata cgtaaccgcg acggaagcga
    17881 tagtggctaa tggcaatccc cagccaggcg ataaaacccg tcatcccgga ggtgttcagc
    17941 agccacaggt ataccgtctg gttgccaaac atggaggtca ggaagcacaa accggcaatc
    18001 accgtcgtcg catacagcgc attacgcggc acgccaccac gcgacaattt agcgaaaatg
    18061 cgcggcgctt taccgtcaca cgccagggtg tacagcatac gagtagacgc atacataccg
    18121 gagttacctg ctgacagcac cgccgtcaga ataactgcgt tcatcaccgc cgccgcagag
    18181 agcagacccg cgtgctggaa caccaaggtg aacggactga cgctgatgtc tttaacatcg
    18241 ttacgcagca ggctcggatc ggtatacgga ataatcaggc tgataatcag gatcgcgaac
    18301 acatagaaca acaggattcg ccagaatacc tgacgtaccg cgcgtggaat gtttttcgcc
    18361 ggatcttcgg actcgcctgc agcaataccg atcagctcgg ttccctggaa agagaagccg
    18421 acaatcatag ctacgccgat catcgccgca aaaccaccag caaacggcgc ttcgccgatt
    18481 gtccagttgc tccagcccgc aggctgcgcg cctttgaaga taccgataat catcagcacg
    18541 ccaacgatga taaagacaat aactgtcgtg actttgatca gtgaaaacca gtattccgct
    18601 tcaccaaagc cacgaactga gatgtagttc agcaggaaga taacgccgag gaacaacgca
    18661 ctccagatcc agcccggtgt atccgggaac caccagctca tgaccagctg tgctgcaacc
    18721 aagtcaacgg caatagtcac cgcccagttg taccagtagt tccagcccag cgcgaagcca
    18781 aagccttctt caacatagtt ctgaccgtaa gtggcaaacg aaccggaaac cggcatatat
    18841 gcagccaatt caccgagact ggtcatcagg aagtaaacca tcaggccaat cagcatatac
    18901 gagagcaatg ccccgcccgg acctgcctga gaaatcgttg cgccagaggc aacaaaaaga
    18961 cctgtaccga tggaaccgcc aatggcaatc atcgtcaggt gacgcgcctt taattcacgg
    19021 cgtaagcccg gcgcttctgt agttttagtt tcggaaccca tacaaaaatg ctatccatct
    19081 taaaaatgag gcgcgattgt agcagacgat tggcaatcct tccggcagaa atacacggtt
    19141 ataagacacc ttcatgatcg cccagggatt ataagtaaag cagccaatct cttttctggc
    19201 gaatgcatgc tgagggcgct gcgtcacgaa atcgacacag caccagcatg ttcttgtaca
    19261 gcaccttaac gcggtacatt cgcgggatcg caatagtcca gaaagcgccg tagcgcgttg
    19321 gaaaggtgtt tttgccgatg atgtatacgc cacaacgtac gcatcaggcg cgggagaggg
    19381 accgcaactt cacttaatgt gcctgcctgc aattgatctt caatcacacg tcgcgacagg
    19441 caactaattc ccaacccatg acgcaccgca tgtttgattg cctcggagtt acctaattcc
    19501 atcgccatct caaacttcgg taaatgtgac agcaacagat aatcgacaat ctcccgcgtg
    19561 ccggaaccgc gttcacgcag gatccacggc gcagcggcca gctgttctaa ggtgaccgga
    19621 ccacgagcca acggcgaagt cggcgcggcg aaaaccacca gctcgtcttc cagccacggt
    19681 tcagaaatga tttcagtgct gtggcacggt ccttcaataa agccaatatc aacgcggaaa
    19741 tccagcaccg cttgcatcac gtcctggctg ttcccaacgc taagttcaat cggcaactgc
    19801 ggataatcat ggcgataacg ggcgataact gcaggcagaa tgtagttacc gatggtacta
    19861 ctggcataga tacgaatcgc gccgttgtct tcgcgaaaca gttgttcgat ttcaaccgcc
    19921 tgttcaagca atgccaacgc acgcggatag agcagccgcc cgtgttcatt aacaaccagt
    19981 cttttcccca cgcgatcaaa cagttgcacg ccaagctgcc cttccaggtc ggtcaaggct
    20041 gcgctcactg ctgattgcga caacgccagc atcaccgacg cctgggtggt tgatccactt
    20101 ttcaatactt ctgcaaaaac ttccaactgc cggagggtga tgtgcatagt cgcttaccac
    20161 ttataaagat taattataaa tatataatca attttatttt taaaccagtt agtcgtaacc
    20221 ttatgcccgg ttaaaggaga gggttatgac gaatatcacc ttacagaaac aacatcgtac
    20281 actgtggcat tttattccgg ggttagccct gagtgcagtt atcaccgggg tcgccctgtg
    20341 gggtggttcc attcccgcgg ttgcgggtgc cgggtttagt gccctcaccc tcgcaatctt
    20401 gttggggatg gttttaggca acaccatcta tccgcacatc tggaaaagct gtgacggtgg
    20461 tgtgctgttt gccaagcaat atttattacg tctgggtatc attctttatg gcttccgtct
    20521 gacgttctcg caaattgccg atgtcggtat cagtgggatc atcattgacg tgttgacgct
    20581 gtccagtacc ttcctgctgg cttgcttcct ggggcagaaa gtgtttggtc tggataagca
    20641 caccagctgg ttgatcggtg ccggtagcag tatctgtggt gctgccgcgg tactggcgac
    20701 tgagtcggta gtgaaagcgg aagccagtaa agtaaccgtg gctgttgcaa ccgttgttat
    20761 cttcgggacc gtcgcgattt tcctctaccc ggcgatatat ccgctgatgt ctcaatggtt
    20821 tagtccggaa actttcggta tctacattgg ttctactgtg cacgaagtgg cgcaggtggt
    20881 ggcggcaggt catgccatca gcccggatgc ggaaaacgca gcagttattt ccaaaatgct
    20941 gcgcgtgatg atgctggctc cgttcctcat cctgctggcg gcgcgtgtta aacagctgtc
    21001 tggggcgaac agcggcgaga aaagcaaaat cactattccg tggtttgcca tcttgttcat
    21061 cgtcgttgcc atctttaact cgttccacct gttaccgcag agcgtggtga acatgctggt
    21121 aacgctggat accttcctgc tggcaatggc gatggcggcg ctgggtctga ccactcacgt
    21181 cagcgcgctg aaaaaagctg gggcgaaacc tctgctgatg gcactggtac tgtttgcctg
    21241 gctgattgtt ggtggtggtg cgattaacta tgtgattcaa agcgtcatcg cataaaccac
    21301 tacatcttgc tcctgttaac ccgctatcat taccgttttc ctccagcggg tttaacagga
    21361 gtcctcgcat gaaatacatt ggagcgcacg ttagtgctgc tggcggtctg gcaaatgccg
    21421 caattcgcgc cgtcgaaatc gacgcaaccg cgtttgcctt gttcaccaaa aaccaacgtc
    21481 agtggcgtgc cgcaccgctc acgacgcaaa ccatcgatga attcaaagcc gcctgtgaaa
    21541 aatatcacta cacatcggcg caaattcttc cccacgacag ttatctgatt aacctcggac
    21601 atccggtcac tgaagctctg gaaaaatcgc gcgatgcctt tatagatgaa atgcagcgtt
    21661 gcgaacaact ggggctttct ttgctcaact tccaccctgg cagccatctg atgcagattt
    21721 cagaagagga ttgccttgcg cgtattgccg aatccatcaa cattgcgctg gataaaactc
    21781 aaggtgtaac agcggtgatt gaaaacaccg ccggtcaggg cagtaactta gggtttaaat
    21841 tcgaacatct cgcggcgatt atcgacggcg tggaagataa attccgcgtc ggcgtctgca
    21901 ttgatacctg ccatgctttc gctgccgggt atgatttgcg tactccagcc gaatgcgaaa
    21961 aaacgttcgc tgattttgcc cgtattgtcg gctttaagta tctgcgcggg atgcacctta
    22021 acgatgcgaa aagtaccttt ggcagccgcg ttgaccgcca tcatagcctc ggtgaaggca
    22081 atatcggtca tgatgcgttc cgctggatca tgcaagacga ccgtttcgac ggcattccgc
    22141 tgatccttga aaccatcaac ccggatatct gggcagaaga gatcgcctgg ctgaaagcgc
    22201 aacaaactga aaaagcggtg gcctgatcga tgaacgacag ggaaaagcag attcttaaaa
    22261 ttttacggcg taacccgctg attcaacaga atgaaatcgc cgatattctg caaatcagcc
    22321 gctcacgtgt tgccgctcat attatggatt tgatgcgcaa gggtttgatt aaaggcaaag
    22381 gatatatcct taccgagcag gattactgtg tggttgtggg tgcaatcaat atggatattc
    22441 gcggaatggc ggatatccgt tacccgcaag cagcctctca tccaggttct gttcactgtt
    22501 ccgcaggcgg cgtcggacgt aatatcgcgc ataacctggc tctgcttggt cgggatgtgc
    22561 atttaatatc agctattggc aacgattttt acggcgagac attactggaa gaaacgcgcc
    22621 gcgcgggcgt gaacgtttca aactgcattc gtctgcatgg acacagcact gcaacttatc
    22681 tcgctatcgc taataagcag gaagaaacta tcctggcaat caatgacaca catattctgc
    22741 aacagctaac gcctcaactg ctcaatactt cacgggactt gattcgccat gctggtgtgg
    22801 tgctggctga ttgtaatctc acacctgaag ccctggagtg ggtctttacc attgctgatg
    22861 aaattccgat gtttgtcgat accgtttccg aatttaaagc gaacaaagta aaaaactggt
    22921 acagccggat ccacactctg aaaccaacac aaaatgaact ggaaattttg tgggggcaac
    22981 cgattaaaga cgacaacgat cgcatcagag ctgtaaattc gctgcatcaa caaggtgtta
    23041 aacgcatttt tgtttatctg aaagatgaat ctgtattttg cagcgataag gatggagaac
    23101 aatttttgct gaccgcgcca gcgcatacga cagtagacag ttttggtgct gacgatggtt
    23161 ttatggcggg cctggtatat agctttcttg aaggatacag tttccgcgac agcgcccgtt
    23221 ttgcggtagc ctgcgcggca atttcgcgcg ccagcggcag cttaaacaac cctaccctgt
    23281 ctgccgataa cgcgctttca ttagtgccaa tggtgtaaca atgttgccgg attcgacgct
    23341 tgaccgcatc ttatccggct cataaattgc gcactacgct aaaccaataa agaatcctgc
    23401 aatagtagca ctcatcaggt tagaaagtgt cgccgccgcc agcgcgcgta aaccaagttg
    23461 ggcgatttcc ggcgcacggt gtggcgcaac cgcagaaaac gcccccacca ccaccccgat
    23521 agaaccaaag ttagcgaaac cgcacaacgc gaaagaaata atcgccacgg ttttagcatc
    23581 cagagtgcca cccgtttgca gatatggcga gaaattgaga taagcgacaa attcattgat
    23641 cgccagcttc tgcccaatca aactcccggc aagatttgca tcactccagt caacccccat
    23701 cacccacgcc aatggggcca acaggtaacc taaaatcgac tccagcgaag catgttcaaa
    23761 accaaaccag ccgccaacac cgccgataat accgttaatc aacgcaatta ttgcaacaaa
    23821 tgccataaca actgtcgcta caccggcggc gattttcagc ccggtcattg cccctgtcgc
    23881 agcggcttca ataatgcttt ttggcggtgt ttcggtgaaa gagaggttat taaaagaaac
    23941 ctgcgaagat tccgtagccg ggcttaacag gcgggcaaac aagatcccgc cagggatcgc
    24001 cattaacgat gccgccagca aatattccac aggtacgccc agtgcggcgt aaccaatcat
    24061 tgtcgaacca gcaatcgagg ccatgccact acaaatcgct gtaaataatt cattgcgatt
    24121 cagacgatcg ataaagggtt tgacgatcgc cggaatttcg ttttgcccga ggaaaatggt
    24181 ggtgaccgcg acgaatgact cgatcttgct gatatttaaa gctttctgga agataccgcc
    24241 gagaatgcga attaagatcc ccatcacacc gatgtagtag agaatactca ccagtgcagt
    24301 gacgaagata attgccggta ataccctgaa accaaagata aatcctgcgc catcaaataa
    24361 ggtatccatt ttcggtccga ccagagaacc gaagataaat gcgctacctg cgtcgctgta
    24421 cgccatcact ttgtggacgc caaaagcgac tttttctgcg acccaacgcc ctggcggtaa
    24481 ccaaagcata atcccgccaa tcacgacctg taacactaac gccgcgccaa cggtacgcag
    24541 gctgatcttc ttcttgttta ctgacagtaa aaacgcaatt gccagcagta ccaccattcc
    24601 cagaacactt ctcatgacat ccatagtgat tatctcttca tgtcattttg ggcgacggct
    24661 ttctgcccat cagaaatagc cgggcctgca tcttgcgata ctgtcccggc ttgttatcaa
    24721 cacttaatgg gtttttatgt agccgcgtac gcactcttcg actaatcccc agaaccagtc
    24781 ggtatcaata gtgatgccga ctttagtatt ggcgggcttg ccaagaacgc ccagctcgtc
    24841 gcagacggta cgcccatagc aagggccact gttgacgtcc acttcgacgt acatctcctg
    24901 ggttttaatg ccatcagggt taatcagata accgatgcag gtggcgtcgt gcaccgggcc
    24961 gccagccagg ccgtagtttt cgaactgcgt tttgagagtg aagttcatga tgtcgctgaa
    25021 catctctccg gcagggccgc ctgccctttc cattcgtgca attacgtccg gggtgcaaac
    25081 ggtctggttg gtaagatcga ggcccatcat cactaatgga acgccggagg tgaacaccac
    25141 gcgtgcggct tccgggtcgg caaagatatt gaactcggca gatggggtga agttgcctgt
    25201 accgtaagcg ccgcccatta gcacaatttc acggattttg ggcaggattg cgggttgcat
    25261 acgcattgcc accgcgatat ttgaaagcgg accaaccggc accagagtga tatcgccatc
    25321 gcttgccata agggtatcaa tgatgtattt caccgcgtgg gtactttctg cctggcgggt
    25381 cagcggctcg aataccgggc catccagtcc ggtttcaccg tgaatattat cggcaacgat
    25441 ttgttgacgc ataatgggtt gcggcatccc cgcataaacc ggaacattaa tctccagttt
    25501 ctggcaaaca ttcaggccat taattaatgt tttatcaagc gtctgattac ccgcaacaat
    25561 ggtgatacct aataaatcaa tcgccggatg tttcgccgcc atcattatag caatagcatc
    25621 atcatgaccc ggatcacaat ccagaataat ttttctcttt tccattgttt atttcctctg
    25681 tttccagttg cgttattttt tctacagcaa cgaaatttcg atcgggccga tgattgaatc
    25741 ttaacaacag cgtacgaatg ctaaatatga gaaatctcat agcggataaa catcgtgaaa
    25801 gaaatccaca ataatgatct taagcagcaa ttgatgagtg aatccgcgtt taaggattgc
    25861 tttttaacgg atgtttcagc cgatacgcgg ctgtttcatt ttttagcgcg tgactacatt
    25921 gtgcaggaag ggcaacagcc gtcctggctg ttttacctga cgcgaggccg cgccaggctt
    25981 tacgccacgc tagctaatgg tcgcgtgtcg ctgatcgatt tctttgccgc cccctgtttt
    26041 attggcgaga ttgagttaat cgacaaagac catgaaccgc gtgcggtgca ggctattgaa
    26101 gagtgttggt gccttgcgct ccctatgaaa cattaccgcc cgctgttatt aaacgacacg
    26161 ctatttttac gaaaactctg cgtcacctta agtcataaaa attatcgtaa tattgtttct
    26221 ttaactcaga atcaatcatt tccgttagtg aatcgcctgg cagcatttat attactctcg
    26281 caggaaggtg atctttatca cgaaaagcat acgcaagcgg cagagtattt aggcgtttct
    26341 tatcgacatc ttttatatgt tctcgcgcaa ttcattcacg acggtttatt aattaaaagc
    26401 aagaaagggt atctaattaa aaacagaaag cagttgtcag gactggcgct ggagatggac
    26461 ccggagaata aattctccgg gatgatgcag taaaaattat tggcaatagc acgattgccg
    26521 gatgcaacgc ttaataaggc gtacatccgg caatgaaact caagctaaac caataaagaa
    26581 cccggcaatg gtcgcgctca tcaggttgga aagcgtcgcc gccgccagcg cccgtaaacc
    26641 aagctgggcg atttccggcg cgcgttttgg cgaaatagcc gaaaacgctc caacgacaac
    26701 accgatagaa ccaaagtttg caaacccaca gagggcgaac gagataatgg caatggtctt
    26761 gacatccagc gtgccgctcg tttgcagata gggcgagaaa ttgagataag caacaaactc
    26821 attaatcgcc agtttctgcc caatcaaact tccggcaaga tttgcatcac tccagtccac
    26881 ccccataatc catgccaacg gggccagcac atagccgaaa atgctttcca gagagacatt
    26941 ggcaaaacca aaccagccgc caactccacc aataatgccg ttgatcagcg caatgatcgc
    27001 tacaaatgcc attaccaccg tcgccacacc ggcggcgatt ttcagcccgg tcattgcacc
    27061 gttcgccgca gcttcaataa tgctttttgg cggtgtttcg ctaaacgaca gattttcaaa
    27121 cgtaacttgc gaaggttccg tcgccggact aagaatacgt gcaaacagga taccgccagg
    27181 aatagccatc agcgaagcgg ctaacaaata gtcaatcggc acccccattc cggcatagcc
    27241 aatcatcatc gaacccgcaa tagacgccat gccgctacaa atagccgtaa atagctcgtt
    27301 gcgattcatg cgatcaataa aaggtttaac gatcgccggg atctcatttt gcccgaggaa
    27361 aatagtggtg actgcgacaa aagattcgat tttgctgatg ttgagggctt tctgaaaaat
    27421 actgccaaga atacgaatca gcagccccat tacgccaatg tagtacagca agctgataag
    27481 cgcggtaacg aaaataatcg ccgggagtac gcgaaaggcg aagataaagc ccgcaccgtc
    27541 aaacagaaca tccattttgg gaccgaccag cgaaccaaaa ataaaggcac ttcctgcgtc
    27601 actgtaggac atcactttat ggacgcctaa tgccgcctgc tctactgccc attttcccgg
    27661 gggaaaatag agcatgatgc caccaatggc aatttgcagc agcagcgcag cgccaaccgt
    27721 gcgcaagctg atgctctttt tatttaccga caacacaaag gcaattgcca gcaataccac
    27781 catccccgca acacttctca ttatatccat aatgattttc ccttcatgcc agtaaacccg
    27841 gcgtcagcgc caggttttgg tatgtttgat gagtacgggc gacggctttc tgcccgccag
    27901 aaattaaccc acgagacgct gatattcttt ggcaatttcg ctcgccagaa tggcgttatt
    27961 aaagaccagt tgaatattgg atttcaggct atcaccgccg gttagctccg caacacgcgc
    28021 cagcaggaac ggtgtactct ctttcccaat aacaccctgt tcttcggctt cagcaacggc
    28081 ttgatcaatt gctgcattga tggtttcttc tggcatcgca aactgttccg ggatcgggtt
    28141 cgctaccacc aggccgccgt tcaaaccggt ttgccacttc actgccatcg cgcgagcaat
    28201 ttcgcgggca ctgtcaagac gaatgctgac ttcaaatggg ctggttcggc agaaaaacgc
    28261 aggcagcgct tttgtctgat aaccaattaa cggtacaccg aaggtttcta aatattctgt
    28321 tgttaatcct aaatcgagaa tagattttgc ccccgcacaa acaacagtga cattggtatt
    28381 tgccagttcc tgtaaatcgg cagaaatatc gaaggtatgt tccgctccgc gatgcacacc
    28441 accaatacct ccggttgcaa aaactttaat cccagccagt gctgcaataa tcatggttga
    28501 tgcaacggta gtcgcgccat ttttccctgc agcaacgaca aaaggtaaat cacgacgact
    28561 aactttcgtc acgttatgtc cttcacggcc cagcaattcg atttcttctt tacttaagcc
    28621 cactttcatc acaccgccaa taatggcgat agtggctggt actgcgccct gtttacgaat
    28681 tgtttcttct acttcaattg cggtttgggc attttgtggg aatggcattc cgtgagaaat
    28741 tatggtcgat tccagcgcaa caaccggttt tttatttttt aaagcatcct gtacttcagc
    28801 ggatatttgt aataattcag gggacagagt taattcagac attctgtgtt ctccactaac
    28861 gatctaacat tggcaattga taattcaggg ttgttggtat attcacaggc aagtgccatt
    28921 gacgaacatc cctgcgcaaa tcgaacagaa tcgataaacg gcataccgtc tacccaacag
    28981 gaggccagcc ccgccatcat ggcgtcgcct gccccagtaa cattgatgac attcgtttta
    29041 ataggggccg accacccact ttcaccgttg atatcactgt agtaaacgcc atcccctccc
    29101 atgctcagca ccaagcggtt caggccgtgt tgatgaaacc aggcggcgac ctttgcgaca
    29161 tcttcacgtc cggaaagtgc aattccgctc aacgtttcgg cttcaagacg gttaggtttt
    29221 aacgtatgga ttttgctcag atgatcgcgg attttgacgc atttccatgc cgataccgga
    29281 tcaataaata caggcgtttc cccgctattt tccagaaccc agaccagcac ctcttcacta
    29341 aggttgcaat ccgcgacaat aacctttgca ccgcgaataa actcctgatg ctgggcaaga
    29401 aattctgcag aaatacagtc actaatactc atatcattta tcgcgacaag catttctcct
    29461 gtattatcga gcagtgataa atagctggat gtattctcac ccggaacaat taagcattta
    29521 tcgacataaa caccggattg attagtttgc gccagtaatg attgcccata aaaatcacca
    29581 cctactgcac ttaaaagcca ggcatttttc cccagtaacg ccagattatg agcgatattc
    29641 cttcccacgc cacctggggt aaatttaatt tttccgggat tcgaatccgc ataattcaat
    29701 ggatgatgtg aatacccagc cacatcaata ttcgccgaac caataattac tatgtagtcc
    29761 ttttcgcgca tagcacgccc cctggcaata aattgccaac gaagaaattg ggtaaaatag
    29821 ccattacagt caataagact attagagcac gtgttcagat ttaaacacat gttcataata
    29881 agcatattta acgtaatgta aatcgatcag aaaaaacgaa atcggccaga tatgaaggcg
    29941 gtcacaaagt ttgtctattt atgcagcaac aaaagagaaa gttgtgatgt gtaacacaca
    30001 atatggcagg caagaaaaag ggcagagatg tttctgccct ccagattgtt gacaacgcgc
    30061 gctttgttca tcctggatgt ggcgtaaacg ccttatccga cctacaaaag cgcaaaaaat
    30121 tcaatatatt gcagggttca tgtaggtctg ataagcatag cgcatcaggc aattttgcgt
    30181 ttgtcatcat cggggcagag atgtctcatc cccgataatg aagataaatt atgcgttttt
    30241 ttctactatt tgtgtttccg ggcgtttcag gaaggcatag gccaaacccg ccaccagcgt
    30301 accggcaata attgctacca ggtaacccaa taccggcgta atagcgccag ggatcagcag
    30361 aacaaacaga ccaccgtgtg gtgccatcag tttcgcacca atcgccattg agattgcgcc
    30421 agtcagcgcc ccaccaacga tacagcacgg tagcacacgc atcggatcac gagcagcaaa
    30481 cggaattgca ccttccgaaa taaagcacag accaagtacc agagcggctt tgccaccttc
    30541 ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt gccagaccca ttgccagcgg
    30601 tggcaccata cctgccgcca taatcgccgc catcgggcca taggtttgag tactcagcag
    30661 acccacaccg aatgcgtacg ctgctttgtt taccggaccg cccatgtcag tacacatcat
    30721 gccaccgagg atcgccccca gcagaaccgc attcgcagtc cccatggtct gcagccagtg
    30781 agtcaaccca tcgagaatgc cagcaaccgg tttaccgatt aggtagatca tcgccagacc
    30841 gaccaccaga ctggaaatta gcggaatgat caggatcggt ttcagcgcct ccatactctg
    30901 tggcagtttc aattgcgtac tgattaactt cgcaatgtaa ccagccagga agccagcaat
    30961 tataccgcca ataaagccag aaccggtgct gaccgccagc ataccgccaa tcagacccgg
    31021 tgtgaggccc ggacgatcgg caatggagaa ggcaatataa cctgccagta ccggcaccat
    31081 cagcgcaaag gctgaaccac caccaatctg catcagcgcc gctgccaacg tacccggctc
    31141 tttaaacgct tcgataccaa aagcaaaaga aagcgcgata cacagaccac ctgcaacaac
    31201 catcggcagc atgtaagaga cgcccgtcag caagtgacgg taagcgcctg cactctcttt
    31261 cttaccttca gtggtcgctg tttgagcttt gcccgctggt tcatacggcg ttgcttcagc
    31321 aaccgcttta tccagttcct gcgcagtttt cttcagcgcc agaccggtag aggtgcgata
    31381 catcggttta ccagcaaatt tcgccagatc cacttcgata tctgccgcca caatcaccag
    31441 atccgctgct gcgacttctt caggggtgat tgcattgccc gcgccaacag aaccacgggt
    31501 ttcaactttc acccaccagc cacgtttttt cgcttcggtt tcaatggctt cagccgccat
    31561 aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca actacgcgtt tcggaccgct
    31621 ggcagcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa ggtttcgcat ggcctttggc
    31681 ttcactcagg aacagctcag ggtgcgcaac tgcccgggaa atatcaccca gccagacatt
    31741 tttaccgttc agcgcgctgt cattcgggat ggaatcaccg agaacaatcg ccatttctgc
    31801 gtcgttcgga ttgtcgatga tttccagttt tgcttttcgc gccgccgcgc ccagcagggt
    31861 cttcgccata taggcgcgtg cctgaccgag attagcgtca ataatcagca gcgttttcat
    31921 tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac gcgcgccatc attgcggcca
    31981 actgcggacg atcggtaata cccacattgc tttgacttac cgccagggct gcaacagctg
    32041 tcgccagacg cagtgtgtgt tcactggatt cacgcatcag caagccataa atcaggccac
    32101 caaccataga atcccctgcg ccaacggtgc ttacgacatc gactgacggt ggtttggcga
    32161 tccattcgcc ggaggcatta acccaaagcg cgccttcggc acccagtgaa ataacaacat
    32221 gcgcaatacc ttgttcacgt agcgcatgtg cagcttcaat cacatctttc atttcaggca
    32281 gtttacggcc tgcccagatt tccaactcgc ggcggttagg tttcaccagc cacggtgccg
    32341 ctttcaaacc tgctactaac gcttcacggc tactatcaaa gataatgcaa ggacactgac
    32401 tacgcaggcg agtcatccag tcggtgaacg cttccgggct gacgcctgac ggtaagcttc
    32461 cgctgacaca gaccatatcg aactgaccga gccagctcag agaatcagtc acaaagcgtt
    32521 cccagtcggc gagggtgact tcaaaacccg agaagttgaa gtcggtcact tcgccgtctt
    32581 tttccgtcag cttaacgtta attcgggtgc gcccctgtac aacctggaaa cggttggcaa
    32641 tgcccagctc gctgaacagt tgctgaaaac catcctgatt gtctttaccc aggaagccgc
    32701 caacggtgac atcaattccc aggtctttta atactttggc cacgttgatg cctttacccg
    32761 ccgcatgcag accggtggtt ttcaccaggt tcacttcgcc gcgttcaatt tccgggcaga
    32821 aaccaacaag gtcataagcc ggattaaggg tgatcgtagc aacacgtctg ctcattatgc
    32881 gccctcccca agaccagcag cgatagcgtc gccaatggct ttcagcgcct gttcagcatc
    32941 tgcaccctgg gcggtaaagc gtaggcgatg acctttctta acgccaagtg ccacaacttt
    33001 catcagacta cgtccgtttg ccggtttgcc ggtgccatca aggtttgtca cggtaatatc
    33061 actgttaaat tgtttaatgg tattgaccag catggtacct ggacgagcat gcaggccgtg
    33121 ttcattgcgc accacaaact ccgcgcttaa cacgtcgtcg gtcggcgcat catcgctggt
    33181 cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag cggtcagctt tattgtcgag
    33241 caataaatca gcgagacgct taagaaccgc gatgggctga tcgtcattca tcgccacact
    33301 caccagcatg gctgccgttt cgccgtccac atcaaaagca tttgccgcac ggcttaccgc
    33361 aatcgcgcta cgcagattgc cttcggcgct atcgctcagc cagataccct gtccgagatt
    33421 cagcggttgt tcattgatgg ctttggtgac gaaagtggcg tcaactgccc ccacctcttt
    33481 cagacgcgca gcgttcagcg cctgaagagt cagcagatcg ctggcgacga tatccagcgt
    33541 cagcatttcg ttgtcgagct tcagctgctc actctgcttt tcgcccatca gtaatgcgcg
    33601 aagttcttct gctgttgttg ctgacttcag ttgttcagca acggaatcat cgctcagtac
    33661 gtgggtcagc tggcgtagca ggcccagatg ttcatccgag ctggcagcaa taccgattgc
    33721 cacgtacgct acctgaccgt caccccaggt gacgccttcc gggaactgaa atacctgaac
    33781 gccggttttc agcacctgat cgcgggtgtc ggtagtgccg tgtggaatag caataccatt
    33841 gccgaggaac gttgaggttt gctgttcgcg cgccagcatg ccattgacgt agccttctgc
    33901 tacattaccg gcctgcacca gcgccgcagc gacctggcga atcgcctctt ctttgtctcc
    33961 ggccttttcg cccggatgga tgtcctgtac ggataactgg aacatagttc tcctctcttg
    34021 ctgaattgaa acgattcagc ctctatgaga aaaaaagcgc caacctggct tagggttaaa
    34081 gacaagatcg cgctgaaacg tttcaagaaa gcataatact tctgtttcag cacgcaagga
    34141 aagccgtaaa gttatgagca aaagtttgat gtgctgcaca attttttgtc atttttccta
    34201 attgctgatg ggaaaatccg ttatcagcaa tttcatttca gcagcgtcag cagccaatca
    34261 gcaacagtga aatggcattt tgattattcg tggcgctttt gatttcgctg tctgtttatt
    34321 ttctgacaag cagcgtaaac tccgcgtctt cctcttccag tgatcgacct gcatgcataa
    34381 ctcccccgca gtctccagcg cgaaatcgtt tgacctgacc tcgacggcgt ttttaatcgt
    34441 tgcctttctc accggtattg cgggtgctct gcaaaccccg acactcagta ttttccttac
    34501 cgatgaagta catgcccgtc cggcgatggt gggattcttc tttaccggca gcgctgtcat
    34561 tgggattctg gtaagtcagt ttctcgctgg gcgctctgat aagcgcggcg atcgcaaatc
    34621 gctgattgtc ttttgctgcc tgttaggcgt gctggcctgc accctttttg cctggaatcg
    34681 caactacttt gttttgctat tcgttggcgt ctttcttagc agctttggct cgaccgctaa
    34741 cccgcaaatg tttgcccttg cccgtgaaca tgccgacaaa accggacgtg aggcggtgat
    34801 gttcagctct tttttacgcg ctcaggtttc actggcatgg gtcattggcc caccgctggc
    34861 ttatgcgtta gcgatgggtt tcagctttac ggtaatgtat ctgagcgcag cggtagcgtt
    34921 tattgtttgc ggcgtgatgg tgtggctgtt tttaccgtcg atgcaaaaag agcttccgct
    34981 ggcgaccggc acggttgaag cgccgcgccg taaccgtcgc gatacgctac tgctgtttgt
    35041 catttgtaca ttgatgtggg gctcgaacag cctgtacatc atcaacatgc cgctatttat
    35101 tatcaacgaa ctgcatcttc ccgagaaact ggccgatgtg atgatgggga ccgccgccgg
    35161 gctggaaatc ccgacgatgt tgattgccgg atatttcgcc aaacgtctgg gtaagcgttt
    35221 cttaatgcgc gttgctgccg tgggtggcgt ctgtttttac gcaggaatgc tgatggcgca
    35281 ttcacctgtc attctgttgg gcttgcagct gctaaatgct atttttattg gcattctggg
    35341 cggcatcggg atgctctatt ttcaggatct gatgcccggt caggcgggtt cagccaccac
    35401 gctctatacc aacacttcgc gcgtgggctg gatcatcgca ggatcagtgg cgggcatcgt
    35461 cgccgaaatc tggaattatc acgctgtgtt ctggtttgcg atggtgatga ttatcgccac
    35521 tctgttttgc ttactgcgga ttaaagatgt ttaaggcgcg gtcagcattt ccagatcgat
    35581 aagccaggtc atggcctgct gacgcgagtt accgcacatt tcagcgctgg cttgtaagcc
    35641 tgcacagact ttcggtcgca gtggcgaggt gaaaatttta cagcgttgct gttcatcaag
    35701 ctgaatgcag ggcgtattgg cgggcttgcc atctggcata ccgggaatag ggctggaaat
    35761 tgaaggggca gtgcaacacg ccccgcaacc cggacggcat tccatacgtt gttctcttaa
    35821 ggttaaccac cggattgatg agcgcgcaca gtaccacctt ttttgcacca gcaaaagtgc
    35881 gaataccact tgcccgaaag gcccgtcgcg agtactttgt cgcgatattt ttgacatttt
    35941 cgactacagg aatttttcga tgccaagagc gaacgaaatt aaaaaaggta tggtactgaa
    36001 ttacaacggc aaactgctgc tggttaagga tattgatatt cagtcgccca ctgcccgcgg
    36061 tgccgctacg ctgtacaaaa tgcgtttttc tgatgtccgt accgggctga aagtagaaga
    36121 gcgtttcaaa ggtgatgata tcgttgacac cgtgacgctg acccgccgtt acgttgactt
    36181 ctcctatgtc gatggcaacg aatatgtctt tatggataaa gaagactata ccccgtatac
    36241 cttcaccaaa gatcagattg aagaagagtt gctgtttatg ccagagggcg gcatgccgga
    36301 catgcaggtg ctgacctggg acggtcaact gctggcgctt gagctgccgc agaccgttga
    36361 tctggaaatc gttgaaaccg caccaggcat caaaggggca tctgccagcg cccgtaacaa
    36421 accggcgaca ttgagcactg gtctggtgat tcaggtacca gaatacttaa gcccgggcga
    36481 aaaaattcgt atccatatcg aagaacgccg ttatatgggg cgtgctgact aacttcagcc
    36541 gcatgcagaa aagggatagc tcaggctgtc ccttttttaa tttattatac caatcttcta
    36601 ttttgcgctt cctgaacacc ttacgccctc aattttcact cgttgattga tcgccctcac
    36661 acttcatcgc attaacaatc cagaccaatt tcaattgctg tcatataact ttacactgtc
    36721 attgttaatt tatcgttact aagacgtgac tcctatgaag acaattgcct ccgttacgct
    36781 cccgcatcat gtacacgctc cacgctacga tcgccagcag ttgcaatcac gtatcgttca
    36841 ttttggcttt ggcgcctttc accgcgctca tcaggcgtta ctgaccgatc gtgtgctgaa
    36901 tgcccagggc ggcgactggg ggatctgtga aatcagcttg ttcagcggtg atcaactgat
    36961 gagccagctc cgcgcacaga accatttata taccgtgctg gagaaaggtg cggacggcaa
    37021 tcaggtgata attgtcggtg ccgttcacga atgccttaat gcaaaactgg attccttagc
    37081 ggcaattatt gagaaatttt gcgagccaca ggtggcaatt gtttccctga cgattaccga
    37141 aaaaggctat tgtattgacc cggccaccgg tgcactcgac accagtaatc cgcggattat
    37201 tcacgatcta caaacccctg aagaacctca ctccgcaccg ggtattctcg tcgaagcact
    37261 gaaacgccgc cgtgagcgcg gccttacacc gtttaccgtg ctctcctgcg acaatattcc
    37321 cgacaatggc catgtggtga aaaacgcggt gctgggaatg gcagaaaaat gttcgccaga
    37381 actcgccggg tggataaaaa aacacgtcag ttttccggga accatggtcg accgcattgt
    37441 tccggctgca accgacgaat cactggcaga aatcagccag catctggggg taaatgatcc
    37501 ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtt gaagataact tcatcgctgg
    37561 gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat gatgtcctgc catgggaaga
    37621 gatgaaactg cgtatgctta atggcagcca ctcttttctc gcttatctgg gttacctctc
    37681 aggattcgtc catatcagtg attgcatgca ggatcgcgta tttcgccatg ctgccagaac
    37741 attaatgctg gatgagcaag cgccgacact gcaaattaaa gatgtcgatt taacacaata
    37801 tgcggataag ttaattgcac gttttgctaa tccgtcgctg aaacataaga cctggcaaat
    37861 cgcgatggat ggcagccaga aattaccgca acgtatgctg gcaggtattc gcatacatct
    37921 ggggcgtgaa acggactggt cgttgctggc attaggcgtt gctggctgga tgcgttacgt
    37981 cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc gatccgctta gcgataaaat
    38041 tcgcgaactt gttgcgggca gcagcagtga acaacgcgta accgccctgc tttccctgcg
    38101 tgaagttttc ggtgatgatc tgccagataa cccgcatttt gtgcaggcca tcgaacaagc
    38161 ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta ttaaacaccc tcaaaattta
    38221 acgatttctg cggttaaagc ggatgaagct caccttcgtc cgctctcccc ttctcttttc
    38281 tgcctttttt agccaggatt aacgctcagt taacttacca gagtattgcg gagccgttgt
    38341 gaccaggacc aacctcatca ccggttttct cggcagcggg aaaaccacgt cgattcttca
    38401 tctgttagcc cataaagatc ccaacgaaaa atgggcggta ctggttaatg aatttgggga
    38461 agtcggaatt gatggtgctt tgctcgccga tagcggcgca ttgctgaaag agatccccgg
    38521 cggctgcatg tgctgcgtta atggtttacc catgcaggta gggttgaata ccttactgcg
    38581 tcagggaaaa ccagaccgct tgttgataga gccgaccggg ctgggccatc cgaaacagat
    38641 cctcgatctg ttaaccgcac cagtctatga actgtggata gatctgcgcg ccaccttgtg
    38701 cattctcgat ccacgcctgc tgctggacga aaaaagcgcc agcaatgaaa acttccgtga
    38761 ccagctggct gccgcagaca tcattgtcgc caataaatcc gaccgtgcga cgcccgaaag
    38821 taagcaagcg ctacagcgtt ggtggcagca aaatggtggc gatcgacaat taattcacag
    38881 tgagcatggg aaagttgacg gtcatcttct ggatttgccg cgtcgcaatt tagccgagtt
    38941 gcccgccagc gccgcgcatt ctcatcagca tgtcgtgaaa aaagggttag cagcgttaag
    39001 cctgccagag catcaacgct ggcgtcgcag tctgaacagc gggcaaggat atcaggcctg
    39061 cggctggata ttcgacgctg atacggtatt cgacaccatt ggcattctgg aatgggcgcg
    39121 acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt cccgaagggc tggtacgaat
    39181 caaccgtcag ggcgatgacc tgcacattga aacgcaaaac gttgcgccac cggacagccg
    39241 tattgagctg atttccagca gcgaagctga ctggaatgct ttgcagagcg cgctgttgaa
    39301 gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt tcagtaagat aattagagaa
    39361 aatatgatta aaaatttgcc gcaaatagtg ttgttgaata ttgtcggcct cgcgctgttt
    39421 ctttcctggt atattcccgt taatcatgga ttctggttgc cgattgatgc ggatattttt
    39481 tatttcttta atcagaaact ggtcgaaagt aaggcctttt tgtggctggt tgcattgacc
    39541 aacaatcgcg ctttcgacgg ttgttctctg ctggcgatgg gaatgttgat gctgagtttc
    39601 tggctgaaag aaaacgcccc tggcagacga cgtatcgtga ttatgggtct ggtcatgcta
    39661 ttaactgcag tggtattaaa ccagctgggt caggcattaa ttcctgtaaa acgggccagc
    39721 ccaacattga cttttaccga tattaaccgc gtcagcgaac tgctctctgt tcccacgaaa
    39781 gatgcctcac gagatagctt tcccggcgat cacggcatga tgctgcttat tttttcggca
    39841 ttcatgtggc gttatttcgg caaagttgca ggccttatcg cccttattat ttttgtggtt
    39901 tttgcatttc ccagagtaat gattggcgca cactggttta ctgacatcat tgtcggttcg
    39961 atgaccgtga tattgatcgg tttgccctgg gtgttgctga cgccattaag tgatcgatta
    40021 atcacctttt ttgacaaatc actaccggga aaaaacaaac atttccaaaa caaataactc
    40081 acagtaatta acatcatcag gcttattttt atagtgagga taatcctgat gatgcgcacc
    40141 gtgctttcat ccatcgcgcg caaaaatcat tctctaagta aatgaatgga ttgcatgcgt
    40201 ttcactcaat cgtactttaa ttgaccaatc ccgcttatta actttctgta tcactttttc
    40261 ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc aatcgggtaa tctagaactc
    40321 gttttgcctc ggcggtagat tatcctcaca gcatataatt ttgtgcgtta gtccacagat
    40381 ttggccttaa ggaattgttt caacatgccc aggtaattag tctcgtgtcg cttggcattt
    40441 ttttataacg atatttgtcg ttaaggactt caagggaaaa caaacaacat ggtcaaatct
    40501 caaccgattt tgagatatat cttgcgcggg atccccgcga ttgcagtagc ggttctgctt
    40561 tctgcatgta gtgcaaataa caccgcaaag aatatgcatc ctgagacacg tgcagtgggt
    40621 agtgaaacat catcactgca agcttctcag gatgaatttg aaaacctggt tcgtaatgtc
    40681 gacgtaaaat cgcgaattat ggatcagtat gctgactgga aaggcgtacg ttatcgtctg
    40741 ggcggcagca ctaaaaaagg tatcgattgt tctggttttg tacaacgtac attccgtgag
    40801 caatttggct tagaacttcc gcgttcgact tacgaacagc aggaaatggg taaatctgtt
    40861 tcccgcagta atttgcgtac gggtgattta gttctgttcc gtgccggttc aacgggacgc
    40921 catgtcggta tttatatcgg caacaatcag tttgtccatg cttccaccag cagtggtgtt
    40981 attatttcca gcatgaatga accgtactgg aagaagcgtt acaacgaagc acgccgggtt
    41041 ctcagccgca gctaataaac cgtttggatg caatcccttg gctatcctga cgagttaact
    41101 gaaagcactg cttaggcagt gcttttttgt tttcattcat cagagaaaat gatgtttccg
    41161 cgtcttgatc caggctatag tccggtcatt gttatctttt aaatgttgtc gtaatttcag
    41221 gaaattaacg gaatcatgtt catacgcgct cccaattctg gacgtaagct cctgcttacc
    41281 tgcattgtag caggcgtgat gattgcgata ctggttagct gccttcagtt tttagtggcc
    41341 tggcataagc acgaagtcaa atacgacaca ctgattatcg acgtacaaaa gtatctcgat
    41401 acctattttg ccgacctgaa atccactact gaccggcttc agccgctgac cttagatacc
    41461 tgccagcagg ctaaccccga actgaccgct cgcgcggcgt ttagcatgaa tgtccgtacg
    41521 tttgtgctgg tgaaagataa aaaaacattc tgttcatctg cgactggtga gatggacatt
    41581 ccactcaatg aattgattcc ggcgctcaac attaataaaa acgtcgatat ggcgatctta
    41641 cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca tctggtatcg caaccctttg
    41701 ctgaaaaata gcggtgtgtt tgccgctctg aatctcaacc tgacgccttc tctcttttat
    41761 agttcacggc aggaagatta cgatggcgtc gccctcatta ttggcaatac tgcgctatct
    41821 accttttctt cacgtttgat gaacgttaat gaattaaccg acatgccggt ccgtgaaact
    41881 aaaattgcgg gcattcctct gaccgttcgg ctttatgcgg atgactggac atggaacgat
    41941 gtgtggtacg catttttact gggcggcatg agtggaactg tcgttggcct gctctgctat
    42001 tacctgatga gcgtacgtat gcgccccggc agagaaatca tgaccgccat caagcgcgaa
    42061 caattttacg tggcgtatca accggtggtg gatacacaag ctttgcgagt aacgggcctg
    42121 gaagtactgc tacgctggcg gcatcctgtc gcgggagaaa ttcccccgga tgccttcatt
    42181 aactttgccg aatcgcaaaa gatgattgtg ccgctgactc agcacctgtt tgagttaatt
    42241 gcccgcgatg ccgcagaatt agaaaaagtg ctgccagtag gcgtcaaatt tggtattaac
    42301 attgcgccgg accatctgca cagcgaaagc tttaaagcag atatccagaa actgctcact
    42361 tccctgcccg cacaccattt ccagattgtg ctggaaatta ccgagcgcga tatgctgaaa
    42421 gagcaagaag ccacacaact cttcgcctgg ctgcactcgg tcggcgtaga aattgctatt
    42481 gatgacttcg gcaccgggca cagcgcgctt atctatcttg agcgttttac gctcgattat
    42541 ctgaaaattg accgtggatt tatcaacgcc atcggtacgg aaacgatcac ttcacccgta
    42601 cttgacgcgg tgctgacgct ggcgaaacgc ctcaatatgc tgacggttgc tgaaggggtc
    42661 gaaacgccgg aacaggcgcg atggctaagc gaacgcggcg ttaatttcat gcaaggctac
    42721 tggattagtc gccctttacc gctggacgat tttgtccgct ggctgaagaa accgtatacg
    42781 ccgcagtggt aaggtgtgct tacgtccctt attattcata gtgaaagcat gccggattgc
    42841 ggctaatgat gagtaaaagg aaatccattg cagatgattg tgcgcatact gctgctgttt
    42901 atcgctctgt tcacctttgg tgtgcaggcg caggctatca aggaaagcta tgcctttgcc
    42961 gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt ttgattatgt gaaccccgcc
    43021 gcgccaaaag gtgggcagat aacgttgtca gccctcggca ccttcgataa tttcaaccgc
    43081 tatgcactgc gcggcaaccc gggcgcacgc accgagcagc tgtacgacac gctatttacg
    43141 acttccgatg acgaaccagg cagttattac ccgctgattg ctgaaagcgc acgctatgct
    43201 gacgattatt cctgggtgga ggtcgctatt aatccgcgcg cccgttttca tgatggttcg
    43261 cccattactg cccgcgatgt agagtttact tttcaaaaat ttatgaccga aggcgtgccg
    43321 caatttcgtc tggtctacaa aggcaccacc gtcaaagcca ttgcaccgtt aaccgtgcgc
    43381 attgagttag ctaaacccag caaagaagat atgctgagtc tgttttcgct gccgatattt
    43441 ccagaaaagt actggaagga tcacaaactt agcgacccgc tcgccacgcc tccgcttgcc
    43501 agtggtccgt accgcgttac gtcctggaaa atggggcaaa atattgtcta ttcccgcgtg
    43561 aaagattact gggcagcaaa cttaccggta aaccgtggac gctggaattt cgacaccatt
    43621 cgctacgatt attacctcga tgataatgtc gcctttgaag cgtttaaagc aggtgccttt
    43681 gatttgcgta tggaaaacga cgctaaaaac tgggccacgc gttataccgg taaaaatttc
    43741 gataaaaaat acatcatcaa agatgagcaa aagaacgaat cagcccagga tacgcgctgg
    43801 ctggcgttta atatccaacg tccggtattc agcgatcgcc gggtccggga agcaatcact
    43861 ctcgcctttg actttgaatg gatgaacaag gcgttgtttt acaacgcctg gagtcgcacg
    43921 aacagttatt ttcagaatac cgaatacgcg gccagaaatt accccgacgc cgcggagctg
    43981 gtgcttctgg caccaatgaa aaaagatcta ccgccagaag tcttcacaca aatctaccag
    44041 ccgccggtat ctaaaggcga tggctacgat cgtgacaacc tgttaaaagc cgacaaactt
    44101 ctcaacgaaa cgggctgggt gctgaagggt cagcaacgcg ttaatgctac aacgggtcag
    44161 ccgctcagct ttgaattatt gcttcccgca agcagcaata gtcagtgggt attgccgttc
    44221 cagcacagcc tgcaacggct gggtatcaat atggacattc gcaaggtgga taactcgcaa
    44281 atcactaacc gcatgcgcag tcgcgactat gacatgatgc cgcgcctatg gcgggcgatg
    44341 ccgtggccca gttccgattt acagatttcc tggtcatcgg aatatatcaa ttccacttat
    44401 aatgcccccg gcgtgcaaag cccggttatc gactcgctga tcaaccaaat tattgccgcg
    44461 cagggaaata aagaaaaatt actgccgttg gggcgagcac tggatcgcgt attaacgtgg
    44521 aattattaca tgctgccaat gtggtacatg gcggaagacc gtctcgcctg gtgggataaa
    44581 ttctcccaac ccgctgtacg ccctgtttac agcctgggta tcgatacctg gtggtatgac
    44641 gttaataaag cggccaaact gccgtcagcc agacaacagg gagagtagat gggcgcttac
    44701 ctgattcgcc gtctgttgct ggtgatccca acgttatggg ctattatcac tatcaacttt
    44761 ttcatcgtgc aaattgcgcc tggcggtccg gtcgatcagg ccatcgccgc cattgagttt
    44821 ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg ttcgtgccag ccatgcgcaa
    44881 acgggtgtcg gcaatatcag cgacagtaat taccgtggcg gacgcggatt agacccagaa
    44941 gtgatcgctg agatcactca tcgctacggt ttcgataagc cgatccacga acgttacttc
    45001 aaaatgctct gggactacat ccgctttgat tttggtgata gcctgtttcg cagcgcctcg
    45061 gtgctgacgc tgattaaaga cagtctgccg gtttccatca ccctcggatt gtggagcacg
    45121 ctgattatct atctggtgtc gattccgtta ggcattcgca aagctgttta taatgggagt
    45181 cgctttgacg tctggagtag cgcatttatc atcatcggct acgccattcc ggcctttttg
    45241 tttgccatcc tgctgattgt cttcttcgcg ggcggcagct atttcgacct gttccctcta
    45301 cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt atcagaaaat caccgattat
    45361 ctgtggcata tcacgctacc ggtgctggcg acagtgattg gtggctttgc ggcgctgacc
    45421 atgctgacca aaaactcatt ccttgatgaa gtgcgcaagc aatacgtggt gaccgcgcgt
    45481 gcgaaagggg taagtgagaa aaacattctc tggaaacatg tgttccgcaa cgccatgctg
    45541 ctggtgattg ccggttttcc ggcgacgttt atcagcatgt tttttaccgg ctcgctgctg
    45601 attgaggtga tgttttcact caatggtctt ggcttactgg gctacgaagc gaccgtctcg
    45661 cgcgattatc ctgtaatgtt tggtaccttg tatattttca ccctgattgg cctgctgctg
    45721 aatattgtca gtgatatcag ctatacgctg gttgatccgc gtatagattt tgagggacgt
    45781 taatgtcgcg actcagcccc gtcaatcagg cccgttgggc gcgttttcgt cataaccgtc
    45841 gcggctactg gtcgttatgg attttcctcg ttttgtttgg tttgagtttg tgttctgaac
    45901 ttatcgctaa cgataaaccg ttgctggtgc gttatgacgg cagttggtat ttcccgttgt
    45961 tgaaaaacta cagcgaaagc gattttggcg gcccgctggc aagtcaggct gattatcagg
    46021 acccgtggct gaaacaacgg ctggaaaata acggctgggt actgtgggca ccgattcgct
    46081 ttggtgctac cagtatcaac tttgctaccg ataagccctt cccttctcca ccctcccggc
    46141 aaaactggct gggaacggat gccaacggcg gcgatgtgct ggcacgcatt ctctatggca
    46201 cgcggatctc ggttctgttt ggcctgatgc tgactctctg ttccagcgtg atgggcgtgc
    46261 tggcgggggc gctacaaggc tattacggcg gtaaagtcga tctctgggga caacgcttta
    46321 ttgaagtatg gtcggggatg ccgacgctgt ttttgattat tttactttcc agcgtcgtac
    46381 agcctaactt ctggtggctg ctggcaatta ctgtcttgtt tggctggatg agtctggtcg
    46441 gcgtggtgcg ggcggagttt ttacgtactc gtaatttcga ctacattcgt gcggcacagg
    46501 cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat gttgcctaat gccatggtcg
    46561 cgaccctcac ctttttaccg tttattttat gtagttcgat aaccaccctg acctcgctcg
    46621 atttcctcgg cttcggtcta ccgctcggtt caccgtcact cggtgaactg ctgttacaag
    46681 ggaaaaataa ccttcaggcc ccgtggcttg ggatcaccgc cttcttgtcg gtggcgatat
    46741 tattgtcttt gctgatcttt attggtgaag ccgtccgcga cgcatttgat cctaataagg
    46801 cggtgtagca tgacgcaaac tctgttagcg attgaaaatt tgtcggtggg ttttcgccat
    46861 cagcaaaccg tacgtacagt agtcaatgat gtttcactac agattgaggc tggcgaaacg
    46921 ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta ccgcgctgtc aattttacgc
    46981 ctgctccctt ccccgccggt tgaatatctc tccggcgata ttcgttttca tggcgaatcg
    47041 ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg gtaataagat cgccatgatt
    47101 tttcaggaac cgatggtgtc gttaaatcca ttgcataccc tggaaaaaca gctttatgaa
    47161 gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc gtggcgaaat tcttaactgc
    47221 cttgatcgcg tcggtatccg ccaggcggca aaacggctga cagattatcc gcatcagctc
    47281 tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc tgttaacgcg accggaatta
    47341 ttaattgccg atgaaccgac caccgcactg gacgtctctg tccaggcgca gattttacag
    47401 ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc tgtttattac tcataacctc
    47461 agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc aaaacggtcg ctgtgtcgag
    47521 caaaataacg ccgctacgct atttgcctca cccactcatc cttacacaca aaagctactc
    47581 aacagtgaac cgtcaggcga tccggtaccg ttgccagaac ctgcctcaac gttgctggat
    47641 gttgaacagc ttcaggttgc cttccccatt cgcaaaggga ttttgaagcg cattgtggat
    47701 cataatgtgg tggtgaaaaa catcagtttt acgctacggg cgggtgaaac actgggttta
    47761 gtgggcgagt ctggttccgg gaaaagcacg acgggactgg cgctgctgcg actgattacc
    47821 tctcagggca gcatcgtctt tgacggtcag ccactgcaag atttaaatcg ccgccagctg
    47881 ttacctactc gtcatcgtat tcaggtggta tttcaggatc caaactcctc gctcaaccca
    47941 cgactcaacg ttttgcagat tattgaggaa ggcttacggg ttcaccagcc gacgctttct
    48001 gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg aagtgggatt agatcctgaa
    48061 acacgccacc gttatccggc ggagttctct ggtggtcagc gacaacgtat tgcgattgcc
    48121 agggcattaa ttcttaagcc ctcactgatc atacttgatg aaccgacatc atcactcgac
    48181 aaaacggtac aggcgcaaat attgacgcta ttgaaatcat tgcaacaaaa gcatcaactg
    48241 gcctatttgt ttatcagcca cgatttgcac gttgtccgcg cgttatgtca tcaggttatc
    48301 gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg cgcgcgtgtt tgccgcaccg
    48361 cagcaggagt atacgcgtca gctactggcg ttgagctgac gcttaaaaag gattgtagtt
    48421 tgaaaagggt tcggcaattg ccacaccaaa atttttcaat cggcatgttg cagcaaactc
    48481 gtcctggcga tttacaaaca ggcacggctc accttcacac tccagcaccg aacatggaac
    48541 ttcgatgtcg cttaacgcct ggcttagttt atacatcacc gaccatgcat tatcaccatc
    48601 cgggctaagc aatttaagcg ccaccaggct gttgtcaccg cacggtccat tttgcggaat
    48661 cggttcaacc ttcgaacctg cgaaaccagc agaccagcga aagttctgcg gtagacgatg
    48721 gacgattgag agttgtaatg aagtcactta ttttccccgg aagcacattt acttcacaat
    48781 ttatttacat caattttaac acatcatcaa caatccgtct ttatacagat cgtaaagatt
    48841 gatgcctcgc ctgcatggcc tctatggctc tgatttaagt aatatctgcg ggcgtggtca
    48901 cgtttgcggg gctatatgta cccgtttctg cgatctaact caaccttttt aactacaatg
    48961 atgtgacttt ttacataaat tgattttaca taaaataaac atatatcggg ggaatgatag
    49021 atttgtggtt gacatgcatc aacaaaggaa gccttttagc ttcctcgttg tgcaatagat
    49081 caccgttttt tcggtcgact ggcgtacaga cagaagagaa tggagctggt tgcgcagaat
    49141 gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag caatgcgcca
    49201 acaattgccc ctatgccaaa acggaaggtt cctgccagcg aagatgccgt tcccgccata
    49261 tggggaaact catcaagaat gaccgccatc gcattggatg acaccataga cacgcagccc
    49321 acaaacgccg caacgccaac caccagcgac cagaatccca gccccagcag cgcactgatg
    49381 accatccacg ctgccataat aaattgtatc cacaaccccg agcggaacat atttaacgcg
    49441 ccaatgcggc ggacgaagcg gctgttaaag atggtcatca cgaacagaaa aacaatgttt
    49501 agcgcaaagt aataaccaaa gttttccggt gcaacgtggt tgatttcaat gtagacaaac
    49561 ggtccggcgc tcaggaacga gaacattccg gcaaagctga aaccgctggc cagcatataa
    49621 ctcaggacac gcttatgacg gaacagcgcc gcaaagttac caatagtggt acgaatgtga
    49681 aaaggctggc gaggatccag aagatgtaat gccagctcag ccacaccagc acccagccgc
    49741 caactatcgg tgccatcagc ggtgcaatgg tcgtcaccag catgacaaac gacatcatcc
    49801 gcgagaactc ttctttcggg taaatatcac gcatcaaagc gttgataacc acgctggccg
    49861 cagccgcagc cagcccgtgg aagaaacgca tcacaatcag ctgatcgatg gtttgtgcca
    49921 acgcacacgc caccgcggcg gcggcaaaca ccagcgtacc gccaagaacc accggcttac
    49981 gcccgaagct gtctgccatc ggcccgtaga ttaactgccc caacgcaaag cccagaatat
    50041 aagtactcag ggtcatctgc gtactgcccg ccggtacgcc aaactgcgct gaaattaccg
    50101 gtagcgcggg cagatacata tcaatcgaca gcggcatcaa catggccagc aggccaagga
    50161 taaaaacaat agcaaacgac gaatgctgtc gggtggtcac aacgggctcc tgaaagtcat
    50221 tgaaaagtta gacgacgctg gcaatttctt cttcagttaa cggacgatat tcaccggggg
    50281 ctaaatcagc atccagcgta atgccgccaa tacgttcacg atgcagctca accacatggt
    50341 tacccacggc ggcgaacatg cgtttcacct gatgataacg cccttcgctg atggtcagac
    50401 gaacctgcgt tggggtaatc acttccagca ccgccggctt agtgagatct ttttcgttat
    50461 gcagctgcac gcctttagca aattgttctg ccgtatcgtc agctacgggt gataccagcg
    50521 tcaccagata ggtcttctcg caatgatggc gcggagaagt aatgcggtgc gaccactgac
    50581 catcatctgt cattagtacc agaccggtgg tatcgatatc cagccgccct gcggcgtgca
    50641 gtttccacgc taccggttca tctagaaaat agagcaccgt tgggtgatca gggtcgtccg
    50701 tggagcaaac ataaccctga ggcttattga gcatgaagta acgtggaccg tgttgctgcg
    50761 ccagcgggtt gccatcgtaa gcgacatcat gttcaggaag cagtttgaac gctgcattac
    50821 ggacgatttc gccatcgacg gtgacacgat tgccgcggat ttcacgcccg gcaatagcac
    50881 ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg catgtgtgtg attttgcctg
    50941 taaaaaacgg aggtcgggta atgacccgaa tatctgaact gttgtctgcc cagtatagcg
    51001 gtctaataac atccctcaag ggaaaaagat ccgtggcata ctattagcag aataatctac
    51061 ctacgcgaga ccatgatttt tacacttcgc ccatatcagc aagaagccgt ggatgccacg
    51121 ctcaaccatt ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggtgcaggt
    51181 aaaagcctgg tgatagcgga actggcacgg ctggctcgtg gtcgcgtact ggtgctggca
    51241 cacgttaaag aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc
    51301 gatatttttg ccgccgggct gaagcgtaaa gagagccacg gtaaagtggt atttggcagt
    51361 gtgcagtcgg tcgcccgtaa tattgatgcc tttcagggtg aattttcgct gttgattgtc
    51421 gatgaatgtc accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac
    51481 ctgacaaaag tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg
    51541 ggcaaaggtt ggatctacca gttccattat cacggcatgg tacgcagcga tgagaaagcc
    51601 cttttccgtg actgcattta tgagctgccc ctgcgttata tgattaaaca cagctatctg
    51661 acgccgccag aacgactgga tatgccagta gtgcaatatg atttcagccg cttgcaggca
    51721 cagagcaacg ggctgttcag cgaagccgat ctcaaccgtg agctgaaaaa acagcaacgt
    51781 attaccccgc acatcatcag ccagattatg gagttcgccg aaaagcgtaa aggagtgatg
    51841 attttcgccg cgacggtcga acacgcaaaa gagattgtgg gattactgcc tgccgaagat
    51901 gcagcactga ttactggcga cacccccggc gctgagcgcg atgtgttaat tgaaaatttt
    51961 aaagcccagc gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc
    52021 ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat cagtgagtct ttaccaacaa
    52081 attgtcgggc gcggtctgcg tctcgctccg ggcaagactg attgtttaat tcttgattat
    52141 gcgggtaatc ctcacgatct ctacgcgccg gaagttggta caccgaaagg caaaagtgac
    52201 aacgttccgg tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa
    52261 acgaccgccg acgggacatt gattgaacac tttggtcgcc gctgtcaggg atggtttgaa
    52321 gatgacgacg gtcatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa
    52381 tgtaacgcag aaaatgatat tgccgctcgc cgctgccgcg aatgtgacac ggtactggtt
    52441 gatccggatg atatgttaaa agcggcgcta cgactgaaag atgcgctggt attacgctgt
    52501 agcggcatgt ctttgcagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat
    52561 tacgatgaag acggtgcgga tgtgagtgag cgtttccgtc tgcaaacacc tgcccagcgt
    52621 accgccttcg agcagctttt tatccgcccg catacgcgca caccgggcat cccgctgcgc
    52681 tggatcaccg ccgccgatat cctcgcccag caagccttat tgcgaccccc ggattttgtc
    52741 gtcgcccgca tgaaaggcca gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt
    52801 cgttttcgtc gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg
    52861 attgagtata gaatctcacc cgcttttgca tacgcaaagc agatcactta cctgttgctg
    52921 ggtcgcctgt agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt
    52981 acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc
    53041 aatcatctac ggtggcaaag aagcgccgct ggctatcgag ctggatcacg acaaagtcat
    53101 gaacatgcaa gctaaagctg aattctacag cgaagttctg accatcgttg ttgacggtaa
    53161 agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca
    53221 catcgacttc gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tcccgcgggg
    53281 ttttttgtat ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa
    53341 ggcgttcacg ccgcatctgg cattcagaac acaaaaccga ttaattgccg ccagacgtcc
    53401 ggcgctgcaa ttggtcgcgc aaattcggcg gcgtgccttt aatggtcagg gtatcggtcg
    53461 ccggctccca gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac
    53521 cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct ccggtctgcc gggaagctct
    53581 cttccagttc gtagcctttt tcagccgcaa actccgtgaa gctcacttcg cttacgcctg
    53641 ccagctcttt cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac
    53701 agtagctata aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg
    53761 cagtgaaatc atcaaccgct tgcaacagcc cacggttttg tgctttcgtg ttcaatcctt
    53821 cgctggcccc gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca
    53881 ggaaagtgag atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg
    53941 caacaatatc cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt
    54001 taacacgcat actgctcagg ttgctcagaa ctgccaccag cagatactcc accgccagat
    54061 aacggtagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt
    54121 cacgcaggcg accagttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct
    54181 gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt
    54241 tggcgctata gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca
    54301 gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca
    54361 gagcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc
    54421 aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc attttacaga aggcagcgta
    54481 acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg
    54541 ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac agtgatgaac
    54601 aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg
    54661 acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc
    54721 cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc tctatcaacg
    54781 aagacaaagc gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac
    54841 cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg
    54901 ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct
    54961 ggtcgcattt attcctacgt aagcattatc ggccatttca gcttcctggt gttcgccacc
    55021 tacttgctga tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt
    55081 ttgtccgtca ttctggcaac ggcgggaatg acgctattac tgatcgatag cgaagtcttt
    55141 actcgtttcc atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa
    55201 aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt
    55261 gaactagtgt ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc
    55321 ttcgcgcgcc cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat
    55381 atctgggccg atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt
    55441 tcgtacccga tgacggcgcg acgttttctt gagaagcatg gtctgcttga tgcgcaggag
    55501 tatcaacgcc gtcttattga gcaaggtaat ccagacgccg tttccgttca gtatccgtta
    55561 agcgaactgc gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat
    55621 ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa
    55681 aatatttcgt tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc
    55741 ctgttctatg gtatctcgcc gagctatatg gacggcattc tgtcgacccg tacgcctgcg
    55801 gcattaatta ctgcgcttaa tcagcaaggc tatcagctgg ggttattctc atcagatggc
    55861 tttaccagcc cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc
    55921 acccaatccg acgagcagac cgccacgcag tggatcaact ggctgggcca ctacgcacaa
    55981 gaagataacc gctggttctc gtgggtttct ttcaatggca ctaacattga cgacagcaat
    56041 cagcaggcat ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaac
    56101 cgcgtgctca atgcactgcg tgattctggc aaactggaca atacggttgt gattatcact
    56161 gccggtcggg gtattccgct gagcgaagac gaagaaacct ttgactggtc ccacggtcat
    56221 ctgcaggtgc cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgcgctg
    56281 actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctacatgt cagcacacct
    56341 gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtt
    56401 actgcagcgg ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac
    56461 aataacggta aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca
    56521 cagttaagtt tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta
    56581 attataaatc agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag
    56641 cagccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc
    56701 ggaggttcaa atcctctcgt gccgaccaaa aatcccaaga aaaaaccaac ctttacggtt
    56761 ggttttttta tatctgcaat taatttgata aacagaccgt gacacatcac agcctgttta
    56821 ttttctgtta tcagaacgtc cagaccactc ccgcctgagt attccacggc gattctaccc
    56881 ctgcgccatt gccatagctg actgacaaat gaccgcttaa cgtcggggta aacgatgacc
    56941 ttatcccagc ctgataaacg ccacgggttc cagccacatc attattaaat ttgccatcgt
    57001 cattaacttt cacatggtta gaatcggcgt attcctgacg cacggcggct ttcagccagg
    57061 gttccaacgt cgtaccgttt tgcaggtcca tgtgatagct taccgccgtt cccgcttcag
    57121 cgcgtaatat ccgggtattt cccacatccg cgcgcatgcc gtttgataac gtgtagtcct
    57181 gaccatctgt ggtaaagccg gtaaaggcga gatagggtct aacactccac aatccgtcaa
    57241 cccaacggaa cccgctctca acatgagcac ccgcgccgtt actattgtaa tcgccaaacg
    57301 ctgttgcccc attactcatc ttgccatgga tggtgttggc aaaacggtca actttcacca
    57361 ccccatcaac ataggcaccg ttctgatgct cccaaccggc ataagccccc agggtgtagc
    57421 tatcaatatt gcctttgccg ccgcgatcaa aaccaatatc agaatgagag taatcaaaga
    57481 acaagccgcg aattgtgctg ctttcttcac gggagaaacg gctatcgata ccgagcgtca
    57541 ggcccgtcaa tgtttgctca aagccagctc ccgcatcagt ggtcacgttg ttgcgggtgt
    57601 taattgccga actccacatc gccgtatcgt aattaacgcc ttttacgcta ccaagacgct
    57661 cacgcacggt gtccagttct gcatcaaata ccagcggttg tgctgctgcc atgttcagca
    57721 catcagtggt tgaaggggta atttgcgcgc gattctctgc gaggctccag ctatggttgc
    57781 cattatccag caaggtatat tcatacgtac cgatatcaac aacgcctccg gcattgccca
    57841 acgtgaatgc agcatcaccg ccgcccgtcg ttaccagtgt aaggctatct cctgctgccg
    57901 ggctggcccc cgtgtccgtc acgaatattt tgaaatcacc tgttgcctga ccgatgacgt
    57961 tgagctgatc gctctgatga ttagccatat cggtacgcat ataaaattgc cgtttcccga
    58021 cagggtattg gtggtcaacg taatgaaatt ttccgcactt gccgtcacag atgaacgagt
    58081 cactggctca gccatcataa tcacatttgc gccggacatc gcaaggttgg ctaagttgag
    58141 gcgagtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatgcgc tattttcgag
    58201 cgaaacgtcc gcttggctgg tatccacggc accatgcgtt ctaaaagaag cgccctcaga
    58261 aaccactacc ttacccacca acatagcggg tgcataattt attttcggct tcatgacctc
    58321 aagaatgcca tcattccctc tgactgacac gttaaccatt gtgccagccc agacattagc
    58381 gcggccattg ttgataacca tattttcaga gatggcttta gaggaatacg tgatacttcc
    58441 attctgatct gatcgaccga gatcatatac agcatttgcc tgcactttcg taccagtatc
    58501 ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg cctgagtgtc
    58561 ctccataaca atgagcccgg agccatcatc cagttcataa ttttttgaca caccatcctt
    58621 tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg ttgagacaat
    58681 taatttcccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca taacctccag
    58741 taatcctccc ttttcgatag cggcaaagac ggcaatccca tctttttcaa caattaagcg
    58801 cccgccttta atcgtgggat cattcgccgc
//
LOCUS       DAJTVJ010000009        58386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000009 DAJTVJ010000000
VERSION     DAJTVJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.009"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..161
                     /locus_tag="OO448_000525"
     CDS             <1..161
                     /locus_tag="OO448_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6335830.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="OO448_000526"
     CDS             complement(199..1260)
                     /locus_tag="OO448_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6335831.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="OO448_000527"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="OO448_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6335832.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="OO448_000528"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="OO448_000528"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6335833.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4200)
                     /gene="hda"
                     /locus_tag="OO448_000529"
     CDS             complement(3454..4200)
                     /gene="hda"
                     /locus_tag="OO448_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6335834.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="OO448_000530"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="OO448_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6335835.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="OO448_000531"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="OO448_000531"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6335836.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="OO448_000532"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="OO448_000532"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6335837.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="OO448_000533"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="OO448_000533"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6335838.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="OO448_000534"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="OO448_000534"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6335839.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="OO448_000535"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="OO448_000535"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6335840.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="OO448_000536"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="OO448_000536"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6335841.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14498..14650)
                     /locus_tag="OO448_000537"
     CDS             complement(14498..14650)
                     /locus_tag="OO448_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335842.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14668..14859
                     /locus_tag="OO448_000538"
     CDS             14668..14859
                     /locus_tag="OO448_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6335843.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15170..15688
                     /locus_tag="OO448_000539"
     CDS             15170..15688
                     /locus_tag="OO448_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6335844.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15704..16243
                     /locus_tag="OO448_000540"
     CDS             15704..16243
                     /locus_tag="OO448_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6335845.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="OO448_000541"
     CDS             complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="OO448_000541"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6335846.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="OO448_000542"
     CDS             complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="OO448_000542"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6335847.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19612..20982
                     /gene="xseA"
                     /locus_tag="OO448_000543"
     CDS             19612..20982
                     /gene="xseA"
                     /locus_tag="OO448_000543"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6335848.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20979..21194)
                     /locus_tag="OO448_000544"
     CDS             complement(20979..21194)
                     /locus_tag="OO448_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6335849.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21263..22735)
                     /gene="der"
                     /locus_tag="OO448_000545"
     CDS             complement(21263..22735)
                     /gene="der"
                     /locus_tag="OO448_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6335850.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="OO448_000546"
     CDS             complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="OO448_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6335851.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="OO448_000547"
     CDS             complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="OO448_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6335852.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="OO448_000548"
     CDS             complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="OO448_000548"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6335853.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="OO448_000549"
                     /gene_synonym="gcpE"
     CDS             complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="OO448_000549"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6335854.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="OO448_000550"
     CDS             complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="OO448_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6335855.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28508..29662)
                     /gene="trmG/rlmN"
                     /locus_tag="OO448_000551"
     CDS             complement(28508..29662)
                     /gene="trmG/rlmN"
                     /locus_tag="OO448_000551"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6335856.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="OO448_000552"
     CDS             complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="OO448_000552"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6335857.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="OO448_000553"
     CDS             complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="OO448_000553"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6335858.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="OO448_000554"
     CDS             complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="OO448_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR6335859.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37873..38718
                     /gene="sseA"
                     /locus_tag="OO448_000555"
     CDS             37873..38718
                     /gene="sseA"
                     /locus_tag="OO448_000555"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6335860.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="OO448_000556"
     CDS             complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="OO448_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6335861.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="OO448_000557"
     CDS             complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="OO448_000557"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6335862.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="OO448_000558"
     CDS             complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="OO448_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6335863.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="OO448_000559"
     CDS             complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="OO448_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6335864.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="OO448_000560"
     CDS             complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="OO448_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6335865.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="OO448_000561"
     CDS             complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="OO448_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6335866.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="OO448_000562"
     CDS             complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="OO448_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6335867.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="OO448_000563"
     CDS             complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="OO448_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6335868.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="OO448_000564"
     CDS             complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="OO448_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6335869.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="OO448_000565"
     CDS             complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="OO448_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6335870.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="OO448_000566"
     CDS             complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="OO448_000566"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6335871.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48471..49274
                     /gene="suhB"
                     /locus_tag="OO448_000567"
     CDS             48471..49274
                     /gene="suhB"
                     /locus_tag="OO448_000567"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6335872.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49419..50273
                     /locus_tag="OO448_000568"
     CDS             49419..50273
                     /locus_tag="OO448_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6335873.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50464..51744
                     /gene="csiE"
                     /locus_tag="OO448_000569"
     CDS             50464..51744
                     /gene="csiE"
                     /locus_tag="OO448_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6335874.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51736..52875)
                     /locus_tag="OO448_000570"
     CDS             complement(51736..52875)
                     /locus_tag="OO448_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6335875.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="OO448_000571"
                     /pseudo
     CDS             complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="OO448_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
     gene            54061..55422
                     /locus_tag="OO448_000572"
     CDS             54061..55422
                     /locus_tag="OO448_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6335876.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55419..55937
                     /locus_tag="OO448_000573"
     CDS             55419..55937
                     /locus_tag="OO448_000573"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6335877.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55937..56257
                     /locus_tag="OO448_000574"
     CDS             55937..56257
                     /locus_tag="OO448_000574"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6335878.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56254..57066
                     /gene="hcaB"
                     /locus_tag="OO448_000575"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56254..57066
                     /gene="hcaB"
                     /locus_tag="OO448_000575"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6335879.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57076..58278
                     /locus_tag="OO448_000576"
     CDS             57076..58278
                     /locus_tag="OO448_000576"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6335880.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14271 a        15784 c        14566 g        13765 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgtggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caattgatat atatcaaaca
    14461 aaataaccct gattaatgaa ttattacgtt tatcatgtta attcatcatt attacatcat
    14521 cattgtaaat aattaaatta acttccataa cattaaaata tgtatccact gacgtttttt
    14581 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
    14641 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
    14701 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
    14761 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
    14821 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc ttcactttca
    14881 cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat caaagcggca
    14941 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttggct
    15001 gcggaataaa tataaaatta atatatatgt tgtaatgata tatttttata aattattccc
    15061 tgcgtgaatt ttaataaatt taatctatcc ctttatacgc aatacattta ctttcctctt
    15121 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
    15181 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
    15241 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
    15301 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
    15361 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
    15421 cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg
    15481 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
    15541 tttaacatat aaggaaggca ccaaagtgta tacctccacc caggtgggta aagagtgcca
    15601 gtttacgaca ggtttagccg ttgttattac cacaacgtat aacgaaacgc gtattcagcc
    15661 aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
    15721 ttgcgccatc ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
    15781 ctctgctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
    15841 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
    15901 ggccgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
    15961 actggacgcc gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
    16021 cagcttagag attcagaaac aaaaactgac gctggcgaaa gaagaagccc gcgttaagcg
    16081 agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca
    16141 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
    16201 gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc
    16261 aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
    16321 tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca
    16381 tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg
    16441 aaatcgtacg gcagacgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc
    16501 agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga acgtaccggc
    16561 aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca
    16621 atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccagc
    16681 acacgaacgc caaggcctgg tcccgggaat gggtgacggt acaacatgtc gtacggcagg
    16741 cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc
    16801 aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt
    16861 gctttaccgg ttgcagacgc cgcagattcg ataacgtcag ggtagatggt gccctgcgcc
    16921 agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac gaaaacgcga
    16981 ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga caggaagcga
    17041 tcttctgccg gaacgtgaac aatgttcaga ccaaagtgat cgccaaacat atccagaacc
    17101 tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta
    17161 ccgatagcgc ggtgcagtag cattgcggtt acggaggaat ccacgccacc agagaggccg
    17221 aggatgactt tatcgtcgcc tacctgctca cggatgcggg ctacagcatc gtcgataatt
    17281 ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg
    17341 cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt
    17401 tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt gacgaagtcg
    17461 gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggtttaccg
    17521 tctgcggtca gcgcatcttc gataccgcga accagtgcac tgtcgtttac gacttcaacc
    17581 tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc
    17641 atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc
    17701 ggcgcacgcg ggctgttttc ttcagtagta ctttccgggc cgccggaaag aataatgccg
    17761 cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa
    17821 acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtctaga
    17881 atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa
    17941 acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt
    18001 agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac
    18061 cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca
    18121 gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt
    18181 tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct
    18241 ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag
    18301 atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca
    18361 gcatggaacc taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg
    18421 agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg
    18481 cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga
    18541 tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg
    18601 cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg
    18661 tttcacggat acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa
    18721 cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa
    18781 ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg
    18841 ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca
    18901 gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt
    18961 cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt
    19021 cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa
    19081 cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg cgcagcgtcg
    19141 tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc
    19201 gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt
    19261 cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca
    19321 tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca
    19381 gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga
    19441 tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac
    19501 agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc
    19561 gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct
    19621 tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt
    19681 gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct
    19741 tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc
    19801 cgcaacagca accgccgggt gaccttccgt ccacagcatg gacaacaagt tttagttcgc
    19861 gccaatatta cgctctacga gccgcgcggt gactaccaga taatcgttga gagtatgcag
    19921 ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct
    19981 gaaggtttgt tcgatcagca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt
    20041 gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc
    20101 gatccttctc taccggtgat catctacccc accgccgttc agggcgatga cgcaccgggg
    20161 caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt
    20221 gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg
    20281 gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc
    20341 attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg
    20401 agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg gctggagatg
    20461 gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca tcaccgatta
    20521 cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa
    20581 aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg
    20641 ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc
    20701 attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag cgccacgcgt
    20761 gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg
    20821 cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt
    20881 aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag tgaagttaaa
    20941 aacatccagc cagtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg
    21001 cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct
    21061 tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc aataatttcc
    21121 ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg acattgtgga
    21181 caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata gagggaatgg
    21241 cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc agacgcttac
    21301 gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt
    21361 ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg aagtagttca
    21421 tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga atcaccacaa
    21481 tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga ccgcgtacca
    21541 gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata gaggtcccca
    21601 cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta ccaacaccac
    21661 tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc agacggaagt
    21721 ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac ttattcacca
    21781 caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga tcctgatcgg
    21841 aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggtatcttca attgcctgca
    21901 acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt ttacgtacgc
    21961 cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg tagatactgt
    22021 cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc ttcaccaaga atacggttag
    22081 tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc ggcagacttt
    22141 gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca aattgcgccc
    22201 agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc cacggcagca
    22261 gcacacgctc cagcagactt aatacgccac gaccgtgaga tgcggcgatc gggtagattt
    22321 cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga ccgtcagttt
    22381 tgtttgccac caggaaggtc ggtttttcac gagagcgcag atgtttggca atcgcttcat
    22441 ctgccggcat caagcccgcg cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa
    22501 tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg ccatcaatcc
    22561 cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg tacttacggt
    22621 cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa
    22681 ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt accatgttta
    22741 aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac
    22801 aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac gattaacgtg
    22861 taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca gcggcaaccg
    22921 gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga ccatcttcga
    22981 cgttaatcca gtgcagataa ccttcactgt cacctaccac caggttgcca ttatacagca
    23041 ccggcgaagt cagcaggcga tgcagcagat cgctttgtgt ccacagcgta acgccgccat
    23101 caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga ttgccgtcga
    23161 cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca ctgcgcagat
    23221 caagcgccgt caggttacca ttataggcca gagcgaaaac aacgccgtta acaacgacgg
    23281 gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc tgggaaatac
    23341 gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga ccattatcgc
    23401 cccccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg
    23461 aaggcatatc gaggttaact gtccatttga cagcgccgtc agcttcgttc agcgcttgta
    23521 actgaccgtt actagtgtgg attaacacca gaccgtcgct gaccaccggg cgagaaagtg
    23581 cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc agcgcgtaaa
    23641 cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaca ccgccagaaa
    23701 gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca gaccagattt
    23761 ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct gcatagacaa
    23821 cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg ctaccaacgg
    23881 aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat ggggacatct
    23941 ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta acggaaagca
    24001 gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga caaattatta
    24061 attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc
    24121 catgcactac gcgcaccttg cttatcacct ttgctcaaca atgctttacc acgcaggtcg
    24181 gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag cgcggcatca
    24241 gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc tttgagattt
    24301 tcatcactcg tgtctgccag cccctgttgt aactgggcgg cagctttctc tagttcattt
    24361 ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt atttttattt
    24421 tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct cactgcggta
    24481 accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg gctgttccag
    24541 tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag tgctttgcca
    24601 ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc gtaaatttcc
    24661 acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct acgctatcct
    24721 gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg ccgttagcca
    24781 cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca cgggcaaact
    24841 gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt aattcatcac
    24901 gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa gccaccaggt
    24961 atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact aacaatacaa
    25021 gacgttcgag gcccatcgca aaaccgactg ccggtgttgc acgaccgccc agttgttcca
    25081 ccagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgagg ctgttagtca
    25141 cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc tggtttacgg
    25201 tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt
    25261 cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc acttccggat
    25321 tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca
    25381 gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt
    25441 ccagcgaacc aatagagttc agctcaagag ttacgtgttc gaagataccc agcgcacgcc
    25501 accagcgggc agtgagcata atcagttcag cgtcgatatc cggaccttgc agaccgaaaa
    25561 cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc tcgtgacgga
    25621 acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca tgctcgatgc
    25681 cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg ccattgcgat
    25741 cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga
    25801 atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag ctgccgagca
    25861 cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt
    25921 tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg aatataaaaa
    25981 tgaaccctca acgcttccct caatgtttcg ggagccatgc gggttcaatc atacacggga
    26041 agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc
    26101 ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt
    26161 gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct tgttgccgcc
    26221 ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc agccgataat
    26281 cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt taaccgtacc
    26341 gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttaa tccctcgcga
    26401 acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga ccggatcggc
    26461 cgccagcgat acgcgcagcg tgtcgccgat gccttccgac agcagcagac ctaaaccaat
    26521 ggcggatttt actgccccgc tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg
    26581 ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga cgtcagacgc
    26641 tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa catgacgcat
    26701 ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca gatctttttc
    26761 cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg cacagtcaac
    26821 caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca gacaatcgac
    26881 gccgtattcc gctactttca gcgcaatgcg atagtcgaag tggatgtcag ccaccagcgg
    26941 cacgttaacc cgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga
    27001 gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga ccgttgcttc
    27061 gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac catcgccaat
    27121 cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag cctggttatg
    27181 catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc ggcattgagg
    27241 gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg tttcccttga
    27301 tactggatct gtactgcggc tggcgcacca attttcagtt tatacggtgc ctggccggtt
    27361 aaattcaaat taccgtcttt acgctgcatc ccgctaaaca attttttacc ggtagcatca
    27421 gtgacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc
    27481 ggcgtggtca cgccagcctg atcggttggc aacggcgcag caccatctgg cgttgttgct
    27541 gccgttggta ccggggtcgc ggcggtatca acatttgcct gcgaaggcga aacaaccgca
    27601 ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg cgtgttggtt
    27661 gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac
    27721 gtatttaacg ggacactctg cccctgctcg ctattactgc tcagttctgc cgaagattga
    27781 tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca ccaggcaccg
    27841 ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca gccatcacgt
    27901 tttttgcggc gtttaccgag ggaaaaactc tgcattggcg caacttttgc agcccgtagt
    27961 ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac cagacgcgca
    28021 taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc cttatcttct
    28081 tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc ctgctgacta
    28141 agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc ttcattttgg
    28201 tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat tgagatgccg
    28261 gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt tatcatacag
    28321 tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa cttactgttg
    28381 catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga ccagacaatt
    28441 gcaacttaat acttcattca taaaacatta cgccacggta cataaagtaa ccgtggcgta
    28501 atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta cgcagggtac
    28561 gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatcg atatcatcac
    28621 cacgagtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt gagaaacggt
    28681 cgatacggct gttcgagctg cgtccatacg gcgcacccgg gaacgggttc cacgggatca
    28741 ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg tgttcagtgc
    28801 cgtcgttaac gtggtcaagc atcacgtatt caatagtgac tcggccctga ttggcgttgg
    28861 atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac tttttgttga
    28921 tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa
    28981 cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta gaaagcgtga
    29041 cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc gccggaacga
    29101 cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta gtgattggcc
    29161 gctgaccggt gacttttgct gcgccgacga ttttcgccgc ccgccacacc tggccgataa
    29221 tttccgacac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag aatttacact
    29281 ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct tccgggatat
    29341 acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg
    29401 aacgctgttc ttcaaccact tccggtgcgc ggatctccgc cacctctttc agtttgccgc
    29461 gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag tgatacatcc
    29521 acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa aactcccgca
    29581 tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg acgttttcag
    29641 gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt tatggcccct
    29701 ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc gctgcattgt
    29761 acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt attaacgggt
    29821 gcgcgggcac acttcgcctt cgccgaagaa ataagcgatt tcgcgagcgg cagattcgac
    29881 ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag cgcgcagagt
    29941 accggccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac gctgaacggc
    30001 gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt caaccagacc
    30061 atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgagcctgtt caacggtcag
    30121 gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg caaagatatt
    30181 accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc
    30241 catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag atggcgcgga
    30301 ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt aaaataaaat
    30361 gagttttagc aacaatcagg gacaaaacag actattgcat gacaaatttc actgtcgcga
    30421 tttgccccac gtcatccatc accagcaatt gataatcacc tttatccgtc aaatgcaggg
    30481 tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag cgttcacctg
    30541 cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa
    30601 tcgcgccatc gcgaacgcct gtcagctgca gagggagctg ggcatcgtga ccgtatggcg
    30661 ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg
    30721 gttccagcgg tagcggccag acatttacca tttcttgctt cgcctgcggg cagtcagcgg
    30781 cgacgcgctg ccctttgtca tccagccaga tggggaagcg aatgccatta atcccttcct
    30841 gctctgacag taacagcgtt ggcggctggc tgccatccag cagccaggtt gccaggcggc
    30901 ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag ataacgccac
    30961 gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac agtaagatat
    31021 tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca acaacgggcg
    31081 tgccgtcagg tctgccagtc cagatcccaa tgacatagcg agcgttaacc ccaatcgccc
    31141 aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg acgcgcggca
    31201 aggcactatc cggcaagggt tgcgcttcat cagccataat ccggcgaatg atccacgccg
    31261 cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc
    31321 ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc agttttgcac
    31381 cagcgccgcc gagaatgagt gaaagattcg gcgcagcgcc gttgggcaaa tataacggca
    31441 atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc agcacctgca
    31501 cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac
    31561 cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct tgcagcagtg
    31621 atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt ttgagcaccg
    31681 atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa tcatcgttga
    31741 gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc acgatcatcg
    31801 ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct tccagacgtc
    31861 gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta ccgagcatca
    31921 tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag atgggttctt
    31981 cccttgactc ttttacctgc tcacgggacc acacaccttg cgcagccatc cgttcgagca
    32041 ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga cggctgggcg
    32101 cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca ggcgactttc
    32161 cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt
    32221 taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc aacgcgcgcc
    32281 agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga cgagcaacct
    32341 gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct tgccatgctg
    32401 cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga tcttcataat
    32461 tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga taacgccaga
    32521 tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg accactcgtg
    32581 cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac gctgcaagaa
    32641 aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt aacaagcgag
    32701 gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc cattggggaa
    32761 catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc accgcccgcg
    32821 ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc acaaagcgat
    32881 cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt aagttctgca
    32941 cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctgg ttttccagtt
    33001 ccagacccac aggcagcaga tccacgacta acgcatccgg cacgctgtta ctggctttta
    33061 cctgcaacca caccagcacc agatcgccgc tacgtaacga gtccagcgat ttgctcttac
    33121 catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg
    33181 attgcggata accgctggca tccatacgca accataacgg ctgatcgcca ctgttgctca
    33241 cctgcaaggt gacaagttga tcgctattca gattgctgtt ttgcgctttc tcgcctgtca
    33301 gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac
    33361 gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca ccaaacgcct
    33421 gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg tagcagttta ttttcttcca
    33481 gcaaggagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc agccatatcc
    33541 gctcatcact atttctcggc gttttcagcg ccagcgcaat cgcttcttca ccacgcgtcg
    33601 catcacccat ggttttcagc gcaatgccaa gttgcagcag cggtaaacca gaagcggcat
    33661 ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc tgacgggcca
    33721 gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta tccgcgtacg
    33781 ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta ccccggttaa
    33841 tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt
    33901 aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat
    33961 cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat
    34021 cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa agtgacggga
    34081 acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac tctttgatat
    34141 aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag
    34201 gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg
    34261 taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc cactgcttat
    34321 gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc tgaatttctc
    34381 catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc acacctggcg
    34441 ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg gcggtcaggg
    34501 caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc agacgcgagg
    34561 tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt gcggcaacta
    34621 tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca
    34681 attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg
    34741 ttaccgacag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt ttaccaccac
    34801 gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa
    34861 taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca aagaacgcct
    34921 gccacggatc tggcgtaacg tagtcagtaa tattcagcac accactatca acggcagaca
    34981 ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta attttcacgg
    35041 ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg aggcgacggt
    35101 tttcatcacc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag
    35161 atttatcgcc aggacgcacc accagcgtac tcaaataaag atcatgacga ttccaggttt
    35221 tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct tgccaccaca
    35281 gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc gcggcaatat
    35341 gcaacttaat ggtatcgccg gggcgataac tggctttatc tagtttcagc gtgacacggt
    35401 cgggtcgcac tgcgccgctc ccgtcgctgt tgtcctgcca gctatagcca gcccaaaaac
    35461 gcacgctact gaccgcttca ttcggcgctt tgacttccag acgataagcg ccccactcta
    35521 ccgggaaact gaccttaccg gtttcatccg cctgaagatc cagagtttgt tcattttcga
    35581 tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag ttccagtagt
    35641 aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt ttcacgcctt
    35701 gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt
    35761 tcaccgtgct gtcagtgcga taatcgtaaa ccgatttcaa ggcgaactgc ggacggagcc
    35821 ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc
    35881 ccgattccag caaactgccc tggaaaataa cctgcaatgg ggaatgcgtt tccttccact
    35941 ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga acttcatcca
    36001 gcgtgcggga aaggttttcg gcagctatat cgccgaattc aaaaccaggt aaggctgaca
    36061 cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca ttagcaggcg
    36121 caccgtacag gtagtatccc accacggaga atttcacttc atcattcggc gtaagcgggg
    36181 ttttctcacc ggtcagattc agcgccatac gctctggcat aaaatcttcg acgtggaaat
    36241 cccacatccg gtactggtta tcgcccgtgt tggcgcgaat atgccacata ccggttgccg
    36301 cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga ctaacgacgt
    36361 tcctgagtac ctgcccgtca ggtttaatca cgtctaactt gatgggttga tcgggcaatg
    36421 ctttaccgtc tgcatcgcgc agtaaaccat tgaggattac cgtttcacct gggcgataaa
    36481 gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa
    36541 attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga ccgtctttac
    36601 gtgccagcag taatgccgca tttttatcat tttccagctg cacatgcccc tgagcgtcac
    36661 tggttgcctg agtcagagtc tgtcctttct catttaataa agagacttca attccttgct
    36721 gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga taacggtgag
    36781 ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc
    36841 cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg atatcgccca
    36901 gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt
    36961 agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc gaattgcggt
    37021 attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag aagttaacat
    37081 cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg ccaggcagca
    37141 gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt atagtttttt
    37201 cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca atagtaacga
    37261 tcaaatcacg tttcggttcg aggtggcgta aacgtagctc tttaagatta tctgacagct
    37321 cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg cgtgagaaat
    37381 cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca ccatcaagtt
    37441 ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg gccagttttt
    37501 gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc gctttagtaa
    37561 cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg
    37621 ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt catccctgcc
    37681 acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg ttatgtaatt
    37741 cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac agccaaatca
    37801 gcctctttcg cttgtcgctt ttcactaaac atccgacaat ttagcctaac cccggcaaaa
    37861 atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc cgaacatatt
    37921 gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca ggaagatcgt
    37981 aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt tgatatcgaa
    38041 gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc
    38101 gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta tgacgaaggt
    38161 aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt agagaaagtg
    38221 tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt
    38281 gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc cgtggtgaaa
    38341 gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga tgcccgcccg
    38401 gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg cggacatatt
    38461 cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc
    38521 gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc
    38581 agctgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac gctggatgtg
    38641 ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg
    38701 gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt tggcggtttt
    38761 ttattgctgg tctggttcgc ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg
    38821 cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa agagagttat
    38881 tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca agcgggcgct
    38941 ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg
    39001 cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga
    39061 tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa gtaaataata
    39121 accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc agcaagtaag
    39181 cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt taaagtcacg
    39241 caaaaaacca ccccagcgac gttcatagaa tggcacaata tgttcggtaa taaagtggct
    39301 aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc caggtaatgt
    39361 atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat cggcttcaat
    39421 gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa taaaagcacg
    39481 cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg
    39541 ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct ggctgctcag
    39601 cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt ctttaccggt
    39661 cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca atgtgcgaac
    39721 gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt
    39781 gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat caagcgcact
    39841 atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg tggattccag
    39901 tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc
    39961 ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt tcggggcgca
    40021 aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt tgaatttgca
    40081 gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt acgccgttaa
    40141 cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact gttcaaccgg
    40201 cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat agttctcaac
    40261 aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt
    40321 attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg ccagcggcag
    40381 acgccagaat ggttcgttct cttgtgaggc actcgccagc aaacgaccgg caagcgcatc
    40441 gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg ccccggtgag
    40501 ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca gaccatcggc
    40561 aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac cgttgcgata
    40621 ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg cacagcagag
    40681 gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg ccccggtaac
    40741 cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct gtttgatgct
    40801 gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca ctggcgcttc
    40861 tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg
    40921 tccgacggtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt tggtgatccg
    40981 ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct gtgccagttg
    41041 cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat
    41101 caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact ccactttacg
    41161 cgtgcctttc ggggctttgt aaccttgcca gaatgcccag cagcgatccg catcccagcc
    41221 ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg
    41281 gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat tattaatgct
    41341 gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat
    41401 cgcttctgtc attttagtta tccttctttg taaaagggcc gccagttggc agccccagga
    41461 tttttccgct aaatgattcg cgttgcagaa aggcggcaag tgagtgaagc cccaggagca
    41521 tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc gtgaagcatg
    41581 acgcggaaat tattcggcct cgtccagcca gactaacaaa atcgcttcga ggattttctc
    41641 gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact gatgcatatc
    41701 ggtgaatcga accgttttcg gatcaagatc aggatacgca tcgtacagtg cttcgccaat
    41761 ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc acgcgcatgg
    41821 ttgatagtgt aacgcgggat ttcgactact aaatcttcgt cggtaacacg tgcctggcag
    41881 cttaaacggc tttcgggctc cagtccccag gctttgtcca gcatgtcgtc ttcctgctct
    41941 gagctttctg gcagcgagtc aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca
    42001 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc gtcgagaatg
    42061 gtttcaccgc tattagcttc cagaacagcg ccatcaggac agagatcctg atgaggcaaa
    42121 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
    42181 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
    42241 ttgttcgatg gcgtcaacgt catcgccctg cgccacttca ctcaggtgag cggcagcatc
    42301 gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg
    42361 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc gggcttttac
    42421 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
    42481 accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
    42541 cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg caccaccagc
    42601 cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag
    42661 ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt taaaggtggt
    42721 gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt tctccaccag
    42781 gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca gcatttcgct
    42841 gtctggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
    42901 atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc gttcacgcac
    42961 cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcgt cagcttctac
    43021 acccgcgtct ttaagcgcgc gacgacaagc cagtaaggtt cgttttacca gtggcgcgat
    43081 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt taacggtcac
    43141 ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
    43201 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag
    43261 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca cttcaaacac
    43321 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt cataaacagc
    43381 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
    43441 aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc cctgacgctg
    43501 ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc ctgccagggc
    43561 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
    43621 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
    43681 cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc gtttaacaga
    43741 actaatggtg ttggcggtgt caagcgctgc gttagtacgc gcgtcataac ccaccgaatg
    43801 cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat ggtcggctaa
    43861 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
    43921 accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
    43981 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
    44041 cactgctccg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc
    44101 acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
    44161 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagct
    44221 cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta tgctgctcgc
    44281 tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac ggatgacgca
    44341 gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
    44401 ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
    44461 tcagcgcctg ggtatcgagt tgataacggg caggcaagcc aaagaaggtg aagtaatcca
    44521 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
    44581 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
    44641 accacactca tctttgacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
    44701 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
    44761 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
    44821 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
    44881 gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg taatcgacat
    44941 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
    45001 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
    45061 cagcaatatc ggtatttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
    45121 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
    45181 cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg ccacaggccg
    45241 gtgcccccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac
    45301 gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat tctcctgatt
    45361 ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc tgcttgtaca
    45421 tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact aactcgatgg
    45481 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
    45541 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
    45601 tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc gactcacctt
    45661 caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca
    45721 ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg ccgcgcagac
    45781 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcctcgccc attccgacga
    45841 tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt
    45901 gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag cgcaccgata cctttcgggc
    45961 catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc aggtcgatag
    46021 gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccacga gcacggcaca
    46081 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
    46141 aaacaaggat ggtgtcatca cgcatcgctg cttcaagttc tttcagatcg ataatgccgt
    46201 tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga cggcaggtat
    46261 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
    46321 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
    46381 tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata tctaccgctt
    46441 cttcagcctg ccagccgaaa cggtgagaac gggaggccgg gttaccaaag gttccgtcca
    46501 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
    46561 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
    46621 aaccaggcta ccggctggat gtacgaccgt gtttacggag cgcgatacgc cccggcctga
    46681 ttctaatatc ttgttatctt taacctaact gtttcgggtt tcagcaagtt gacaaactta
    46741 cgaatctccg ggagcttaca taagtaagtg accggagtga gtatgtgcag tcaacgcaga
    46801 tgcaacacga aacaggacgg ttaaattaag cgcgtaactt aacgtcgatc gcgtcttgta
    46861 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
    46921 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
    46981 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcaaccgcct ttaccctgac
    47041 aacgggtagc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
    47101 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
    47161 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
    47221 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
    47281 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
    47341 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
    47401 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
    47461 ccattttttt atcgtggcgt ttgtacctgt tgtccttcca gaccatgggc gggttgaggg
    47521 caattattcg cattagcgct ggcctcttcc cctcacccta accctctccc cgaaggggcg
    47581 aggggaccgt ccgtgcattt atctggctta gccctcaggc cttattacct ttattctgct
    47641 gctctataga agccagaatc ccgcgcagga tattcaattc ctggctttcc gggcgcgcac
    47701 gggtaaacag acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac
    47761 cggttgccag cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg
    47821 gatacggcgt ctcttcatgc tcgacctgct cgccattttc ctgagttgcc agccaggcca
    47881 tacgcacttc ataggcaata acctgaaccg ccattgccaa gttcagcgaa ctgtattccg
    47941 ggtttgccgc aatcgcgaca tgataatggc atttctgcaa ctcttcattg gtcaagccga
    48001 cgcgctcacg accaaacacc agcgccaccg gggtatttgc cgcttcagcg acgcttttca
    48061 gaccgcattc gcgcgggtcg agcatcggcc acggcagcgt gcgggagcgt gcgctggtgc
    48121 ccaccaccag gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac
    48181 caatcacatc gctggcccct gctgccaggg caatcgcctg ggagtcgggt ttcaccagtg
    48241 gattaaccag ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca
    48301 tattgccggt gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc
    48361 ttcatctaaa gattattcac gcatcttatc ataaaacgaa gacagatgcc gatctcgctg
    48421 ctatactctg cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga
    48481 tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc aaaaactatg
    48541 aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg accaacgtag
    48601 ataaagctgc cgaagcggtg attatcgaca cgattcgtaa atcttactca cagcacacca
    48661 tcatcaccga agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg
    48721 atccactgga tggcactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg
    48781 ccgttcgtat caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac
    48841 tgttcaccgc cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggtagca
    48901 ccgctcgcga tctcgacggt accattctgg cgaccggctt cccgttcaaa gcaaaacagt
    48961 acgccactac ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc
    49021 gtaccggttc tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct
    49081 ttgaaatcgg tctgcgtccg tgggatttcg cggcaggcga gctgctggtt cgtgaagcag
    49141 gcggcatcgt cagcgacttc accggtggtc ataactacat gctgaccggt aacatcgttg
    49201 ctggtaaccc gcgcgttgtg aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg
    49261 ctctgaagcg ttaatgactc aggcgggtga tgtcactcac ccgccctcgc ctttcaggcg
    49321 ctattctgaa atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc
    49381 ccgattaatg gatgataaag aagtcacaag gaaaggttat ggcgctgcca gtgaacaaac
    49441 gcgttctcaa aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc
    49501 gcgttgccat caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt
    49561 cggcggaatc cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc
    49621 cttcttcgac gggccctgct gacaatgcca tcgcaaccat cattcatgct cacggcaatg
    49681 ccggaaatat gtccgcccac tggccgctgg tcagttggtt acccgagcgt aatttcaacg
    49741 tttttatgtt tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caggccggat
    49801 tgctggacga tacgcaaagt gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac
    49861 aacggctggt gctgttcggg cagagtattg gcggggcgaa tattcttgct gttattggtc
    49921 aggatgatcg tgaaggcata cgtgcggtga tcctcgactc cacatttgcc tcttatgcaa
    49981 ccatcgccaa tcagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg
    50041 aaaattatat cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc
    50101 acgttatccc atggcagcac agcgaaaagt tgtatagcct ggcaaaagag acaaaacggt
    50161 tgatcctaat cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgatgttt
    50221 atcgcgaaca gatggtggac tttatcctta gcgcgttgaa tccgcagaac taacccatga
    50281 tcgctagcac gataatcatt cacaaaacca ccttaagaca tgctaatcca ctggtcagaa
    50341 cagtttaaga tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc
    50401 ttcgccattt ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta
    50461 cagatgatgc ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag
    50521 atcttgctga cgctctttca gccggggttg accgccacca tggcaacctt cagcgagctt
    50581 aatggtgtgg atgatgatat tgccagtctt gatatcagcg aaacaggacg ggagatcctg
    50641 cgctatcatc aactcacact gacgactggt tatgacggta gctaccgggt tgaaggtaca
    50701 gtgcttaacc aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtccg
    50761 tcatttatta ccagccaatt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt
    50821 gcgcgtaact tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacgg
    50881 ctgcaaaaac gctttgaaac gcgcgatatt catttcctgt gtctgtatct gcaatattgt
    50941 ttgctgcaac accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg
    51001 gaatcctgcc ttgaatttca ggtagcgcag gaaattggac gccactggca gcgtcgggcg
    51061 ctccagcctg taccgcctga tgagccactg tttatggcat tacttttttc catgttgcgg
    51121 gttcccgatc cattgcgtga tgcgcatcag cgagacagac aattgcgtca gtctatcaaa
    51181 cgtctggtaa accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta
    51241 tgcgatcagc tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt
    51301 attgataata ccctgccgga agagttcgcc agactgtacc cacgcctggt gcgcaccacc
    51361 cgcgcggcgc tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagc
    51421 ggtctggtcg cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa
    51481 cagattattc tactgaccgg gaatgatagc gagcgagaag cgcagattga gcagcagtta
    51541 cgcgaactaa cgttactgcc gctcaacatt aagcatatgt cggtaaaggc atttttgcag
    51601 acaggcgctc cgcgtggcgc ggcactgatt attgcgcctt ataccatgcc attaccgctc
    51661 ttttcaccac cgctgatcta tacggacctg acgctgacaa cacatcaaca ggagcagatc
    51721 cgcaaaatgc tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggaag
    51781 cgccaccagc gccattaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc
    51841 gaaaacggtc atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa
    51901 acggatgact tcgctaccct ggcgaacagc aatatagcgc atggcggcca ggtggcagac
    51961 cgtgaaggtg ccgcaatgca aaatttgcac cactatcaac cacggcaacg ccgtagttgc
    52021 tcccataatg ccccagcgca ctacgccgca aatcgccgag atcaacagca tatcgcgagc
    52081 actacaacgg cggaaaagtt tattactcag cgcaaagata atgacttccg ccaccacgcc
    52141 cagcgaccac aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc
    52201 gctaaaaccg taataggccg catgtgcccc ctgcaataaa caaacgcagg ccagaaagcg
    52261 ccagttctgg cgaaccagcg ccaaccacgc agaccaaccg gtgctctcct gctggcgact
    52321 tgccccttgt ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc
    52381 caacgtcaac agcgcgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag
    52441 cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg
    52501 gaactgcttt tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa
    52561 gaggttaaag ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc
    52621 gacagcaaag agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgggaagg
    52681 atcgctgacg cggggcgcga tgagcaaact cccgagaaaa cgggcaacca gacctgcccc
    52741 caataacagg ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa
    52801 aggtagaaaa atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt
    52861 ggattgcaaa accatgagtc cctcccgtca ggaggcgtta gtctggggtt aatcgaatgt
    52921 tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt
    52981 cacaagcact aaaaaactgc gatccgcgac gcatgttgca aaaataatgc gtaattatgc
    53041 cgttacgctt gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat
    53101 ctcccctttc ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg
    53161 aaaaacaacc tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag
    53221 ccccatgccc accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg
    53281 ctggctgttt tcttgcgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc
    53341 cggatcggta ctgacgaaat tcacgccatc cagttacgcg gcggtgatct ctttttcatg
    53401 tgctaaaggg tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa gctccagata
    53461 atcaatctcc gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg
    53521 aattttttcc tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg
    53581 tctgagacga aacattggta atacttttgg caataaattc acttccgccg atggcacaaa
    53641 gccaatggtt aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc
    53701 cgcttgttcg aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc
    53761 gactttgcgc ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat
    53821 ctggctgctt aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag
    53881 tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt
    53941 atcgaaaaaa cgtctcagct ggtagaaatt aactatttca catattagca accaaccaac
    54001 aacatcctta tgacacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat
    54061 atgaccacac cctcagattt aaacatttac caactgattg atacccaaaa tggtcgggtc
    54121 actccgcgta tttataccga cccagatatt taccaactgg agcttgaacg tattttcggc
    54181 cgttgctggt tatttctcgc ccacgaaagc cagatcccaa aacctggtga tttctttaac
    54241 acctacatgg gggaagatgc ggttgtcgta gtgcgtcaga aagacggcag cattaaggcg
    54301 tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact
    54361 cgcgccttta cctgtccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat
    54421 gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta aatcccactg gggactaaac
    54481 gaagttcctt gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca
    54541 ccgggcctgc gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt
    54601 cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgtaactgg
    54661 aaattcccgg cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct
    54721 gccgttcagg tattaggggc gaaagatgat ggcagcgata agcgcctcgg tgatggacaa
    54781 accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc aatttggtca ggacggtcac
    54841 ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt
    54901 tcaagctatt accgcgaaac ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc
    54961 ctgcgcctgg cgggtcataa caatattttc cccacgcttt catggctcaa cggcactgcc
    55021 acgctccgcg tctggcatcc gcgcggccct gatcaagttg aagtgtgggc gttctgtatt
    55081 actgacaaag ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct
    55141 tttggtcctg ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa
    55201 ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag
    55261 gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc agaaactgcc
    55321 gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg
    55381 ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag
    55441 agttacatca ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga
    55501 aatttcgtga ctggctggag cagctcgaca aagagattcg ttacaccatg cgcaccacag
    55561 ttaacgcgca aacacgcgac cgccgcaaag gcgtccagcc accgacaacc tggattttta
    55621 atgacaccaa agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc atggcctggg
    55681 cagaagagcc gccgtcacgc acccgtcact taatcagcaa ctgccagata agcgaaaccg
    55741 acatcccaaa cgtatttgct gtgcgggtaa attatctgct ttatcgggca caaaaagagc
    55801 gcgatgaaac attctatgtt ggaacgcgtt tcgacaaagt tcgccgtctg gaagatgaca
    55861 actggcgctt gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc
    55921 tgagtgtact gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg
    55981 tgaggctctc cggatcgata cctcgcccgt catcgccctg ttcaacgttg gcggcgagtt
    56041 ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag ggtatctgga
    56101 agatgacgcc acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa
    56161 agcgttatgc ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg
    56221 tgacattttc atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt
    56281 tttattaccg gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa
    56341 ggcgcgcaag ttgccacgct ggaactgtcg gcggcaaaag tcgccagtct gcgtcagcga
    56401 tttggcgaac atattctggc ggtggaaggt aacgtgacct gttatgccga ttatcaacgc
    56461 gcggtcgatc agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcaggc
    56521 atctgggatc acaatgcctc actggttaat actcccgcag agacgctcga aaccggcttc
    56581 cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg gcgcaaaagc ctgcgctccg
    56641 gcgttaatcg ccagtgaagg cagcatgatt ttcacactgt caaatgccgc caggtatcct
    56701 ggcggcggtg gcccgctgta caccgccagt aaacatgccg caaccggact tattcgccaa
    56761 ctggcttatg aactggcacc gaaagtgcgg gtgaatggcg tcggcccgtg tggtatggcc
    56821 agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa cctcgataat gcagtctctg
    56881 acgccggaga aaattgccgc cattttaccg ctgcaatttt tcccgcaacc ggcggatttt
    56941 acggggccgt atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg
    57001 atcaacgctg atgcgggttt agcgattcgc ggcattcgcc acgtagcggc tgggctggat
    57061 ctttaaggaa gcacgatgaa agaaaaaacg atcattattg tcggtggcgg gcaagcggcg
    57121 gcaatggctg cggcctcgct acgccagcaa gggttcaccg gtgagctgca tctgttttcc
    57181 gatgagcgac atcttcctta tgaacgacct ccactctcga aatccatgtt gctggaagat
    57241 tccccgcagt tacagcaggt gttacccgct aactggtggc aggaaaacaa tgttcatctg
    57301 cattccggtg taaccatcaa aacgctgggt cgcgacacac gagagttagt gttaaccaac
    57361 ggcgaaagct ggcactggga tcagcttttt atagcaatcg gcgcggcagc tcgaccgctg
    57421 ccgttgcttg atgcactggg agaacgctgc tttaccctac gccatgccgg tgatgccgcc
    57481 agactgcgag aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt
    57541 ggtctggaac tggctgccag cgccacgcag cgcagatgta aggtgacagt gattgaactg
    57601 gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc aacgctatct tttacagcgc
    57661 caccagcagg ctggtgtgcg cattctgctc aataatgcca ttgaacatgt ggtcgatggt
    57721 gaaaaagtag aactgacgct gcaaagtggg gaaacgcttc aggctgatgt ggtgatttac
    57781 ggtattggta tcagcgccaa cgagcaactg gctcgcgagg ccaaccttga tactgccaat
    57841 ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat
    57901 gtggcaatca ctcgtcttga taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc
    57961 aataaccagg cgcaaattgc cgctgccgca atgttagggc taccgctacc gctactgccg
    58021 ccgccgtggt tctggagcga tcagtacagt gataacttac agtttattgg cgatatgcgt
    58081 ggcgatgact ggctttgtcg tggcaacccg gaaactcaga aggcgatttg gtttaatctg
    58141 caaaacggcg tgcttatcgg tgcggtcacg ctgaatcagg ggcgtgagat tcgcccaatt
    58201 cgcaaatgga tccagagcgg caaaacgttt gatgcgaaac tgctgataga tgagaacatc
    58261 gcgcttaaat cactgtaacc aggataatta gcgaatatct caacgcctgg ggcgtggcga
    58321 ggtgcaagag tgtgtattac gtttaaatca cattatcttg caaagggatt ggttatgaac
    58381 acacta
//
LOCUS       DAJTVJ010000010        57819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000010 DAJTVJ010000000
VERSION     DAJTVJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.010"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..326
                     /gene="istB"
                     /locus_tag="OO448_000577"
     CDS             <1..326
                     /gene="istB"
                     /locus_tag="OO448_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCR6335881.1"
                     /translation="ARVLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFA
                     DWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVE
                     SGQHQ"
     gene            533..1876
                     /gene="argG"
                     /locus_tag="OO448_000578"
     CDS             533..1876
                     /gene="argG"
                     /locus_tag="OO448_000578"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6335882.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1884..3491)
                     /locus_tag="OO448_000579"
     CDS             complement(1884..3491)
                     /locus_tag="OO448_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6335883.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3969..4055)
                     /locus_tag="OO448_000580"
     tRNA            complement(3969..4055)
                     /locus_tag="OO448_000580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4019..4021),aa:Leu,seq:gag)
     gene            complement(4070..4402)
                     /gene="secG"
                     /locus_tag="OO448_000581"
     CDS             complement(4070..4402)
                     /gene="secG"
                     /locus_tag="OO448_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6335884.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4630..5967)
                     /gene="glmM"
                     /locus_tag="OO448_000582"
     CDS             complement(4630..5967)
                     /gene="glmM"
                     /locus_tag="OO448_000582"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6335885.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5960..6808)
                     /gene="folP"
                     /locus_tag="OO448_000583"
     CDS             complement(5960..6808)
                     /gene="folP"
                     /locus_tag="OO448_000583"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6335886.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6898..8832)
                     /gene="ftsH"
                     /locus_tag="OO448_000584"
     CDS             complement(6898..8832)
                     /gene="ftsH"
                     /locus_tag="OO448_000584"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6335887.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8932..9561)
                     /gene="rlmE"
                     /locus_tag="OO448_000585"
     CDS             complement(8932..9561)
                     /gene="rlmE"
                     /locus_tag="OO448_000585"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6335888.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9687..9980
                     /gene="yhbY"
                     /locus_tag="OO448_000586"
     CDS             9687..9980
                     /gene="yhbY"
                     /locus_tag="OO448_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6335889.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(10136..10612)
                     /gene="greA"
                     /locus_tag="OO448_000587"
     CDS             complement(10136..10612)
                     /gene="greA"
                     /locus_tag="OO448_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6335890.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10860..12293
                     /gene="dacB"
                     /locus_tag="OO448_000588"
     CDS             10860..12293
                     /gene="dacB"
                     /locus_tag="OO448_000588"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6335891.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12479..13651)
                     /gene="cgtA"
                     /locus_tag="OO448_000589"
     CDS             complement(12479..13651)
                     /gene="cgtA"
                     /locus_tag="OO448_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6335892.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13667..14632)
                     /locus_tag="OO448_000590"
     CDS             complement(13667..14632)
                     /locus_tag="OO448_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6335893.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14759..15016)
                     /gene="rpmA"
                     /locus_tag="OO448_000591"
     CDS             complement(14759..15016)
                     /gene="rpmA"
                     /locus_tag="OO448_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6335894.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(15037..15348)
                     /gene="rplU"
                     /locus_tag="OO448_000592"
     CDS             complement(15037..15348)
                     /gene="rplU"
                     /locus_tag="OO448_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6335895.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15607..16578
                     /gene="ispB"
                     /locus_tag="OO448_000593"
     CDS             15607..16578
                     /gene="ispB"
                     /locus_tag="OO448_000593"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6335896.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16807..17085
                     /gene="sfsB"
                     /locus_tag="OO448_000594"
     CDS             16807..17085
                     /gene="sfsB"
                     /locus_tag="OO448_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6335897.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(17133..18392)
                     /gene="murA"
                     /locus_tag="OO448_000595"
     CDS             complement(17133..18392)
                     /gene="murA"
                     /locus_tag="OO448_000595"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6335898.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18447..18701)
                     /gene="ibaG"
                     /locus_tag="OO448_000596"
     CDS             complement(18447..18701)
                     /gene="ibaG"
                     /locus_tag="OO448_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6335899.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18861..19154)
                     /gene="mlaB"
                     /locus_tag="OO448_000597"
     CDS             complement(18861..19154)
                     /gene="mlaB"
                     /locus_tag="OO448_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6335900.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(19154..19789)
                     /gene="mlaC"
                     /locus_tag="OO448_000598"
     CDS             complement(19154..19789)
                     /gene="mlaC"
                     /locus_tag="OO448_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6335901.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19808..20359)
                     /gene="mlaD"
                     /locus_tag="OO448_000599"
     CDS             complement(19808..20359)
                     /gene="mlaD"
                     /locus_tag="OO448_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6335902.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(20364..21146)
                     /gene="mlaE"
                     /locus_tag="OO448_000600"
     CDS             complement(20364..21146)
                     /gene="mlaE"
                     /locus_tag="OO448_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6335903.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(21154..21963)
                     /gene="mlaF"
                     /locus_tag="OO448_000601"
     CDS             complement(21154..21963)
                     /gene="mlaF"
                     /locus_tag="OO448_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6335904.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            22173..23150
                     /locus_tag="OO448_000602"
     CDS             22173..23150
                     /locus_tag="OO448_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6335905.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            23164..24150
                     /gene="kdsD"
                     /locus_tag="OO448_000603"
     CDS             23164..24150
                     /gene="kdsD"
                     /locus_tag="OO448_000603"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6335906.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            24171..24737
                     /gene="kdsC"
                     /locus_tag="OO448_000604"
     CDS             24171..24737
                     /gene="kdsC"
                     /locus_tag="OO448_000604"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6335907.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24734..25309
                     /gene="lptC"
                     /locus_tag="OO448_000605"
     CDS             24734..25309
                     /gene="lptC"
                     /locus_tag="OO448_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6335908.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            25278..25835
                     /gene="lptA"
                     /locus_tag="OO448_000606"
     CDS             25278..25835
                     /gene="lptA"
                     /locus_tag="OO448_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6335909.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25842..26567
                     /gene="lptB"
                     /locus_tag="OO448_000607"
     CDS             25842..26567
                     /gene="lptB"
                     /locus_tag="OO448_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6335910.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26615..28048
                     /gene="rpoN"
                     /locus_tag="OO448_000608"
     CDS             26615..28048
                     /gene="rpoN"
                     /locus_tag="OO448_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6335911.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            28071..28358
                     /gene="hpf"
                     /locus_tag="OO448_000609"
     CDS             28071..28358
                     /gene="hpf"
                     /locus_tag="OO448_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6335912.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28476..28967
                     /gene="ptsN"
                     /locus_tag="OO448_000610"
     CDS             28476..28967
                     /gene="ptsN"
                     /locus_tag="OO448_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6335913.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            29013..29867
                     /gene="rapZ"
                     /locus_tag="OO448_000611"
     CDS             29013..29867
                     /gene="rapZ"
                     /locus_tag="OO448_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6335914.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29864..30136
                     /gene="npr"
                     /locus_tag="OO448_000612"
     CDS             29864..30136
                     /gene="npr"
                     /locus_tag="OO448_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6335915.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            30350..30982
                     /gene="yrbL"
                     /locus_tag="OO448_000613"
     CDS             30350..30982
                     /gene="yrbL"
                     /locus_tag="OO448_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6335916.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30979..31707)
                     /gene="mtgA"
                     /locus_tag="OO448_000614"
     CDS             complement(30979..31707)
                     /gene="mtgA"
                     /locus_tag="OO448_000614"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6335917.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31704..32357)
                     /gene="elbB"
                     /locus_tag="OO448_000615"
     CDS             complement(31704..32357)
                     /gene="elbB"
                     /locus_tag="OO448_000615"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6335918.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32587..34923)
                     /gene="arcB"
                     /locus_tag="OO448_000616"
     CDS             complement(32587..34923)
                     /gene="arcB"
                     /locus_tag="OO448_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6335919.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(35019..35948)
                     /locus_tag="OO448_000617"
     CDS             complement(35019..35948)
                     /locus_tag="OO448_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6335920.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36623..41083
                     /gene="gltB"
                     /locus_tag="OO448_000618"
     CDS             36623..41083
                     /gene="gltB"
                     /locus_tag="OO448_000618"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6335921.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            41096..42514
                     /gene="gltD"
                     /locus_tag="OO448_000619"
     CDS             41096..42514
                     /gene="gltD"
                     /locus_tag="OO448_000619"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6335922.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42698..43177
                     /locus_tag="OO448_000620"
     CDS             42698..43177
                     /locus_tag="OO448_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6335923.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            43194..43499
                     /locus_tag="OO448_000621"
     CDS             43194..43499
                     /locus_tag="OO448_000621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6335924.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43575..44039)
                     /locus_tag="OO448_000622"
     CDS             complement(43575..44039)
                     /locus_tag="OO448_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6335925.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(44036..44911)
                     /gene="nanK"
                     /locus_tag="OO448_000623"
     CDS             complement(44036..44911)
                     /gene="nanK"
                     /locus_tag="OO448_000623"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6335926.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44908..45597)
                     /locus_tag="OO448_000624"
     CDS             complement(44908..45597)
                     /locus_tag="OO448_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6335927.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45645..47135)
                     /gene="nanT"
                     /locus_tag="OO448_000625"
     CDS             complement(45645..47135)
                     /gene="nanT"
                     /locus_tag="OO448_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6335928.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(47245..48138)
                     /gene="nanA"
                     /locus_tag="OO448_000626"
     CDS             complement(47245..48138)
                     /gene="nanA"
                     /locus_tag="OO448_000626"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6335929.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(48260..49051)
                     /gene="nanR"
                     /locus_tag="OO448_000627"
     CDS             complement(48260..49051)
                     /gene="nanR"
                     /locus_tag="OO448_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6335930.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49431..50753
                     /gene="dcuC"
                     /locus_tag="OO448_000628"
     CDS             49431..50753
                     /gene="dcuC"
                     /locus_tag="OO448_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6335931.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50796..51293)
                     /gene="sspB"
                     /locus_tag="OO448_000629"
     CDS             complement(50796..51293)
                     /gene="sspB"
                     /locus_tag="OO448_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6335932.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(51299..51937)
                     /gene="sspA"
                     /locus_tag="OO448_000630"
     CDS             complement(51299..51937)
                     /gene="sspA"
                     /locus_tag="OO448_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6335933.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(52332..52724)
                     /gene="rpsI"
                     /locus_tag="OO448_000631"
     CDS             complement(52332..52724)
                     /gene="rpsI"
                     /locus_tag="OO448_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6335934.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52740..53168)
                     /gene="rplM"
                     /locus_tag="OO448_000632"
     CDS             complement(52740..53168)
                     /gene="rplM"
                     /locus_tag="OO448_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6335935.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(53387..54514)
                     /gene="zapE"
                     /locus_tag="OO448_000633"
     CDS             complement(53387..54514)
                     /gene="zapE"
                     /locus_tag="OO448_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6335936.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54708..55106
                     /locus_tag="OO448_000634"
     CDS             54708..55106
                     /locus_tag="OO448_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6335937.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            55260..56627
                     /gene="degQ"
                     /locus_tag="OO448_000635"
     CDS             55260..56627
                     /gene="degQ"
                     /locus_tag="OO448_000635"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6335938.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56717..57784
                     /gene="degS"
                     /locus_tag="OO448_000636"
     CDS             56717..57784
                     /gene="degS"
                     /locus_tag="OO448_000636"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6335939.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14393 a        15382 c        14359 g        13685 t
ORIGIN      
        1 atgcccgggt gttgatcctg gatgaaatag gctatctgcc gatgaacaga gaggaagcca
       61 gtctgttctt ccggctactg aaccgtcgat atgaaaaagc gagcatcata ctgacgtcaa
      121 acaaagggtt cgcagactgg ggagaaatgt tcggagatca cgtactggca acagcgatcc
      181 tggatcggct gctacatcac tcaaccacgc tgaatatcaa aggagagagt taccggttaa
      241 aagagaaacg taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa
      301 tggtggaaag cggacagcat cagtaacgaa agtatttagc gggcatgaaa atggcaaata
      361 acggtcaaac atcgtggcgt tgacataagc gcattatttt acaaaaaaca cactaaactc
      421 ttcctgtctc cgataaaaga tgattaaatg aaaactcatt tattttgcat aaaaattcag
      481 tgagagcgga aatccaggct catcatcagt taattaagca gggtgttatt ttatgacgac
      541 gattctcaag catctcccgg taggtcaacg tattggtatc gctttttccg gcggtctgga
      601 caccagtgcc gcactgctgt ggatgcgaca aaagggagcg gttccttatg catatactgc
      661 aaacctgggc cagccagacg aagaggatta tgatgcgatc cctcgtcgtg ccatggaata
      721 cggcgcggag aacgcacgtc tgatcgactg ccgcaaacaa ctggtggccg aaggtattgc
      781 cgctattcag tgtggcgcat ttcataacac caccggcggc ctgacctatt tcaacacgac
      841 gccgctgggc cgcgccgtga ctggtaccat gctggttgct gcgatgaaag aagatggcgt
      901 gaatatctgg ggtgacggta gcacctataa aggaaacgat atcgaacgtt tctatcgtta
      961 tggtctgctg accaatgctg aactgcagat ttacaaaccg tggcttgata ctgactttat
     1021 tgatgaactg ggcggccgtc atgagatgtc tgaatttatg attgcctgcg gtttcgacta
     1081 caaaatgtct gtcgaaaaag cctactccac agactccaac atgcttggtg caacgcacga
     1141 agcgaaggat ctggaatacc tcaactccag cgtcaaaatc gtcaacccaa ttatgggcgt
     1201 gaaattctgg gatgagagcg tgaagatccc ggcagaagaa gtcacagtac gctttgagca
     1261 aggtcatccg gtagcgctga acggtaaaac ctttagcgac gacgtagaaa tgatgctgga
     1321 agctaaccgc atcggcggtc gtcacggcct gggcatgagc gaccagattg aaaaccgtat
     1381 catcgaagcg aaaagccgtg gtatttacga agctccgggg atggcactgc tacatatcgc
     1441 ttatgaacgt ctgttgaccg gtattcacaa cgaagacacc attgagcagt atcatgcaca
     1501 tggtcgtcag ttgggccgtc tgctgtacca ggggcgttgg tttgattccc aggcgctgat
     1561 gctgcgtgac tctctgcaac gctgggttgc cagccagatc actggtgaag ttaccctgga
     1621 gctgcgccgt gggaacgatt attcaatcct gaataccgtc tcagagaacc tgacctacaa
     1681 gccagagcgt ctgacgatgg aaaaaggcga ctcggtgttc tcgccagatg atcgtattgg
     1741 tcaattgacc atgcgtaacc tggatatcac tgatacccga gagaaacttt ttggttatgc
     1801 caaaactggc ctgctctcct cctctgccac ttcaggcgtg ccgcaggtgg agaatctgga
     1861 aaacaaaggc cagtaattcg acatcaaccc tgcccttcag gggtggggtt accttgctta
     1921 tcctctgtaa actcctttct gagcatcaca taatcgaaaa catcatggtt tgcatccaca
     1981 acccgtgagt ctcctttata gtgtgcaatc acttcctcca gcgttttcgg ttgttccggc
     2041 tttgttaccc ccatccacgc attaataagg taattattgt aagctgtcga aaagatgttg
     2101 ttttccgttg tacgatccac gccatcccca agaacggggc tataccagat aaacatcggg
     2161 acatgatatg cctgctggct agcctccctg cctccatgaa aatagacgtt cttcttcgta
     2221 gggtcacgtt ccaggccgtg gtcggcaaaa tacatgaccg aggcgcggcg atcttttaat
     2281 aattcaaaaa cctgacctag caaactatct gtgtaatgaa tggagttgtc atagcaggca
     2341 tcctgatcgt cctgcggttg aaacacggcg ctggattgcg gataggcgct acaagccggt
     2401 tcatggcttc catttaaatg aagaacaatc agtttctttt gctgcgtatt ttgctgtaat
     2461 gcttgcgata aatgcggcaa caacaattca tcaaatcctc tgacataaac tgtttccatg
     2521 gcgcgcatgg cgatactggt aactgctgta ccattctgcc gaaaagcgga ttgcgagctt
     2581 agccagaaag tctgaaatcc tgcttgatta gccatattaa taatgttgtc ggggtaatta
     2641 tgaatgtcat gactcaaaac agaatcagca gttaaagaaa ggggaaccga cagcgcagtg
     2701 taaggtgcgc cgcttattga ttgattaaac agtttgatct gttttctttg tgcttcaact
     2761 tgcggtgtcg tagagcgtgt atatccatac aaagacatat tgtcgacacg tacagactcc
     2821 cccacaatca acacgtaggt atcaatacct gtatccctga ctgataattg aaaatacggc
     2881 accgtgtttg caattgagag taatctttga tgctctttcg ctgctaaagc aaaataattg
     2941 agattgaaaa acggcgtata ggtagcaaat cgcgacgcta gtatatatgg actgaatgct
     3001 tttttatttt ttgcatcttt ataagcaaat tgacaagcag aaaataaact gcccgaaatg
     3061 acaatcagca ataatattcc tgtcactttt tttgtcggca aggaaacatc atattttatt
     3121 attactgcca aaaaaagaag ggataaaaaa gcaaaagcac atagataagg gatatacatc
     3181 cccagcattt tgacaacttc atccggatca ctctgcagca cactaatcgc aaatccatca
     3241 ttgaaagttg taccaaaggt acaccatgaa tagaggctaa tactcatgtc tgccgcagtc
     3301 agtaacacaa atggtgcggc aacaagcagg cgaagcggaa gggatgatat acgttttaca
     3361 accaggatgg tcaggacgaa gaacagcaat cgctgaacca tatgcgcgcc ggaagaggcg
     3421 actaagttcg taataccaaa aacaataaca caaagataca acaaccaatg agatttaaaa
     3481 tcttctagca aatttgttga atactgtcat ccagaaatcc aagttcacaa ccaatataaa
     3541 caattatcaa caatgttcaa ttttatatca gacatgtttt tcaactctga tccatatcag
     3601 accgaaacac tgttagtttt ttctttttaa atgatgcggc cattgcgttc tcagtaatta
     3661 aataacatta tcaatgcgtc tctaaacatt acgcagaacg tagccatcag tagttatact
     3721 gacaggagca ggatggttta tcgagaagta aacatatccg tattcgtacc ggaactggag
     3781 ttgacacaat aaagtgccaa ttatgtcagc agaagggaaa agtttataac taaggcgtac
     3841 cggcaccatc gtttcaaggt accagctacg agtaaagcaa ctggacgaga tacagatacc
     3901 tgacaaccat tcctcagacc aggaccaaaa cgaaaaaaga cgcttttcag cgtctctttt
     3961 ctggaatttg gtaccgagga cgggacttga acccgtaagc cctattgggc actaccacct
     4021 caaggtagcg tgtctaccaa ttccaccacc tcggcacgga tactactttt tagttcggga
     4081 tatcgctggt cggcttagcc ggagcagctg gctgagtttg ttcggttttc gccggtgcac
     4141 tcagattttc ccattcgcta cctttattgg ttttgttgct attgatgtta cccagcacca
     4201 gactgatgat aaagaataac gttgccagca gcgccgtcat gcgggtcatg aagttaccag
     4261 aaccacttga accaaacagc gtagcggaag cgcctgctcc gaaggaggct cccatatcag
     4321 cgcctttacc ttgctgcagc atgatcagac caacaaggcc aattgccaca ataaggaaaa
     4381 ctaccaaaag agcttcgtac ataatcaacc tgttccttgc ggagttgccg cgtaccaatg
     4441 cttcaaccaa taaagcggga gtttttaatc tttcccactg aagtgggtgt gaatactaac
     4501 caaagcgaat gaccttcgca agggcaattt tatcgcattg tatcaactgc ggaaaaaaac
     4561 agcaaaaccg atgtgttcgc tgagaaaaaa ggcgacaatc gccgcctttt tagccagtta
     4621 tttaccgttt taaacggctt ttactgcatc ggcgatacgg tgtgcaaatt cagttacctg
     4681 cgcttcgtct tcaccttcta ccatcacgcg aattaacggt tcggtgccgg atttacgcaa
     4741 cagcacacgt ccacggctac ccagcgccgc ttcaacttcg gcggtcacag ctttaactga
     4801 ctcatgctca agcggatcgc cgctacctgc ggtgtaacgc acgttaacca gaatctgcgg
     4861 gaacattttc atgccgctgc aaaggtcgtg caggctcata tggttacgtg ccatcgccgc
     4921 cagcacctgc aagccagcaa cgatgccgtc accggtagtg gttttatcca gcaggatcac
     4981 atgaccggaa ttctctgcac cgatacgcca gcctttctcc tgcatttttt ccagtacgta
     5041 gcggtcaccc actttcgcgc gcgcaaatgg aattcctaac tgtttcaacg ccagctcaag
     5101 ccccatgtta ctcatcaatg tacccacagc accaccacgc agctggccct gacgaagacc
     5161 ttcacgcgcg atgatataca tgatctgatc accatcgact ttattgcctt catggtcaac
     5221 cataatcacg cgatcgccgt cgccgtcgaa ggcaataccg agatccgctt tttcagccag
     5281 cacacgagcc tggagcgcgc gaacgtcggt agcccccact tcggcattga tgtttacacc
     5341 gttcggctca caaccgatag cgataacgtt cgcccccagt tcacgcagca cgttcggcgc
     5401 gatgtgataa gtcgcaccgt ttgcgcaatc caccacaatc ttcagttcgc tgaggctaag
     5461 ttcgttcggg aacgtggctt tgcaaaactc gatatagcga cccgcagcat caacgatacg
     5521 gctggcttta cccagttctg ccgaatcaac acagctgatc tctttttcca tttccgcttc
     5581 gatggcctct tctaccgcat ccggtagttt ggtgccgtcg atagagaaaa atttaatgcc
     5641 attatcgtag aacgggttat gcgatgcaga tatcacaatt ccggcctctg cgcggaaggt
     5701 acgcgtcaga taagccacgg ccggtgttgg catcgggcca gtgaagagtg cggaaaggcc
     5761 cgctgccgcc agacccgctt ccagtgctga ctccagcata tagccagaaa tacgcgtgtc
     5821 tttaccaata atgatcttac gggagccgtg gcgcgccagc actttacccg cggcccaacc
     5881 cagcttaagc acaaaatcag gtgtgatcgg cgcatcccct acacgaccac gaatcccatc
     5941 ggtaccgaaa tatttacgat tactcatagc gtttgttttc ctttgcagac agagtggctt
     6001 ccaccacccg catcgcttct acggtttctt tgacgtcatg aacacgaata atgtgcgcgc
     6061 cttgcattgc ggcaatgacc gcacaggcca gactaccgct caggcgctcg gacggcccca
     6121 cgttcagcag ctgcccaatc atcgattttc gtgacatacc taccaacagc ggcaggttga
     6181 aatggtgaaa ttcagccagg cgcgccagta atgaatagtt atgggagaga tttttaccga
     6241 aaccgaatcc ggggtcgagc aacaatttct cttttgcgat acccgcctgc tcgcaacgtg
     6301 ctatttgctc aataaagtag cgattcactt ctgcaaagac atcgtcatac ttcggagctt
     6361 cctgcatggt ttttggattt ccctgcatat gcatcagaca aaccggtaaa ccggtttctg
     6421 cagccgcctc cagagcgcca ggttcggaaa gggagcggat atcgttaata atgtgagcgc
     6481 caactttcgc tgactcacgg atgacttctg gtttggatgt atctaccgag atccagactt
     6541 cgaagcgttg agcaattgcc tcaaccacag gaataacacg ttgcaactct tcttcaacgc
     6601 taacttccgc cgcccctggg cgcgtggact cgccaccaac gtcaatgatc gtcgcgccag
     6661 cgttgatcat cagattcgca tgtttcaccg catctatcag cgagttatgc gtgccaccat
     6721 ccgaaaagga atcaggcgtg acgttgagga tccccattac gtgaggatgg ctaaggtcca
     6781 gtgaagtgcc ctgggcaaag agtttcatgg tgttatccct ggtatgaatt gataagaaaa
     6841 aaccccggag cacgccccgg ggttttcggt acaaatacag tcatctgatg cgggaactta
     6901 cttgtcgcct aactgctctg acatggtgtt acccgggttc ggcgtacgcg gttcatcaac
     6961 cggacgagga gcctttggac taccattgtc gccagaattg ttagaagcgc ctggttcttc
     7021 ccagcccgct ggcggacgta catcgcgacg tgccatcagg tcatcaatct gcggtgcgtc
     7081 gatagtctca tatttcatga gagcatcttt catcgcatgc agaatatcca tattgtcggt
     7141 cagaagctga cgcgcacgat tatagttacg ctcaatcagt gctttcactt cctggtcgat
     7201 gatacgtgca gtttcatcgg acatatgttt cgctttcgct acgctacggc cgaggaacac
     7261 ttcaccttct tcttccgcgt acagcagtgg acccaatttc tcagagaagc cccactgagt
     7321 caccatgtta cgcgccaggt tggtcgcaac tttaatatcg ttggacgcac cggtagaaac
     7381 atgttccggc ccgtagatga tctcttctgc cagacgacca ccgtacagcg tagaaatctg
     7441 gctttccagt ttctgacggc tggcgctgat tgcgtcgccc tcaggcaaga agaaagtcac
     7501 acccagcgca cgaccgcgtg ggataatcgt cactttgtgc accggatcgt gttccggcac
     7561 caggcgaccg ataatcgcat gacccgcttc gtggtaagcc gtcgattctt tctgcgcttc
     7621 cgtcatcacc atggagcgac gttccgcacc catcatgatt ttatctttcg ctttctcgaa
     7681 ctcaaccatc gacacaacgc gtttgttgcc acgagcagcg aacagtgccg cttcgtttac
     7741 caggttcgcc aggtcagcac cggagaaacc aggagtacca cgggcaatga ttgccgcgtc
     7801 gatatcgggt gccaatggta cgcgacgcat gtgaactttc aggatctgct cacgaccgcg
     7861 aacatctggc aagccaacca caacctgacg gtcgaaacgg ccaggacgca gcagggccgg
     7921 gtcaagaacg tccggacggt tagtcgcggc gataacgatg ataccttcgt taccttcgaa
     7981 gccatccatc tcaaccagca tctggttcag agtctgttca cgttcatcgt gaccaccgcc
     8041 cagaccagcg ccacgctggc ggcctacggc gtcgatttca tcgataaaga tgatgcacgg
     8101 tgccgctttc ttcgcctgtt cgaacatgtc acgaacacgg gatgcaccca caccgacgaa
     8161 catttctacg aagtcagaac cggagatagt aaagaacgga actttcgctt cgcctgcaat
     8221 cgctttcgcc agcagcgttt taccggtacc cggaggaccg accatcagga cgcctttcgg
     8281 gatcttaccg ccgagtttct ggaagcggct cggctcgcgc agatattcaa ccagttcagc
     8341 aacttcttct tttgcttcgt cgcagcccgc aacgtcagca aaggtcgttt tgatctgatc
     8401 ttccgtcagc atgcgcgctt tgctcttacc aaacgacatg gcacctttgc caccgccgcc
     8461 ctgcatttga cgcatgaaga agatccacac accaatcagc aacagcatcg ggaaccagga
     8521 gatgaagata gaagccagca ggcttggttc ttcaggcggt tcaccgacaa ccttgacgtt
     8581 cttggtcaac aggttatcca gtaatttcgg atcctgaacc ggaatgtaag tggtataacg
     8641 gttactatct ttcttggtaa cgttgatttc acgtccgttg atacgcgctt cacgaacctg
     8701 gtcgttattg acctcttgta ggaaggtaga gtaatccacc ttacggccat tagactcgct
     8761 gggcccaaag ctctggaata ctgacatcag cacaacggca atgaccagcc agagtattag
     8821 gtttttcgcc atgtcactca agggattaac ctcttattac aactgtgtta aaaacagcgt
     8881 caggatactc tatatccagc atctttcaaa ctttcgtctg aaatctcccg gttagggttt
     8941 acgcccggtc gctacaatat acacttcccg cgaacgtgca cgagaagagt ccggcttacg
     9001 aactttgacc ttcgtaaaca gggagcgaat ttcccttaga tactcatcga aaccttcgcc
     9061 ctggaacacc ttcactacaa aactgccacc tggcgctaat acatcacgac acatttctag
     9121 cgccagttcc accagataca tggcacgggg gatatccacc gccggtgttc cgctcatgtt
     9181 tggtgccata tcggacatga caacctggac tttgctgtcg ccaacgcgct ccagcagtgc
     9241 tttcatcacc agttcatcac gaaaatcgcc ctgaagaaag tctacaccaa cgataggatc
     9301 cataggtaaa agatcgcaag cgatgatgcg gcctttgccg ccaatttggg tgaccacata
     9361 ttgtgaccaa ccacccggag cagcaccaag gtcgacaacc gtcattcccg gtttaaagag
     9421 tttgtcactt tgctgtattt catcaagttt aaaccaggca cgggaacgta accccttttt
     9481 ctgtgcctgt tgaacatatt tatcgctaaa gtgttcctga agccagcggc tggagctggc
     9541 agaacgcttc ttacctgtca tttaactttc ccatggggat aactcatcgt aaccaattgc
     9601 gtaaattttt acgcgcctat ttggtgatat atgggagatg gcggtagaat gacccatttt
     9661 caatcccaac gtaagcaaaa aatacgatga atctgagtac taaacaaaaa cagcacctga
     9721 aaggtctggc acatccgctc aagccagttg ttctgcttgg cagtaatggt ttgaccgaag
     9781 gggtgctggc cgagattgaa caagcgttag agcaccatga actcatcaag gtgaaaatcg
     9841 ccaccgaaga tcgcgaaaca aaaaccttga tcgtggaagc tatcgtgcgc gaaaccggcg
     9901 cctgtaatgt acaggtcatc ggtaaaacgc tggtgcttta tcgcccaact aaagaacgta
     9961 aaatctcgct gccacgctaa gattatccta aagttacaca catttgctgt gtaaaacgag
    10021 gggttttccg caggcaggag agcaaaatgc cacgctctgt tcgttgataa aaggccgcat
    10081 agcggccttt ttcctttctt tacaatacat caacatcttg agtattgggt aattcttaca
    10141 ggtattccac cttaattact tcaaattcta cttcgccgcc cggcgttttg atgaccacaa
    10201 catcatcttc ttctttgccg atcaggccac gagcaatagg cgagtttaca gaaatcaggt
    10261 tttgtttaaa gtcagcttcg tcatcgccaa cgatgcgata agtctgttct tcgtcagaat
    10321 ccagattcag caccgttacg gtagcaccaa aaataacgcg cccattgtta ggcattttgg
    10381 tgacatcaat cacctgcgcg tttgacagct tggcttcgat gtctttgata cggccttcgc
    10441 agaagccctg ctgttcacga gctgcgtggt attcggcgtt ttctttcagg tcgccatgct
    10501 cacgcgcttc cgcgatagca gcaatgattt caggacggcg cacagatttc agaaaatcca
    10561 gctcttcgcg taatttttca gcgccgcgta aggtcatcgg aatagcttgc atttgttata
    10621 cctcttgaat attcctgatg gggcaagtct tcacccaccc cggctgttcg gcctgcccgg
    10681 cataactcct caccgggacc agaagcaaaa aaataccgac ccaggtacaa gtcccaggtc
    10741 agctacaatt cacattttga tagtcatttt accctgaagt tcccgaaggg tcatcgttta
    10801 ctttataggg cgttgcgccg tagtatgacg gctcgattcc aggttgttag cgcgagatta
    10861 tgcgattttc cagatttatc atcggattga ccagctgtat agcgttcagt gttcaggccg
    10921 caaatgttga tgagtacatt actcaactcc ccgctggtgc caaccttgcc ctgatggtgc
    10981 aaaaagtcgg cgcgtcggcc cccgctattg attaccacag tcagcagatg gcgctgcctg
    11041 ccagtaccca gaaagtgatt actgcgctgg cggcgttgat tcaactcggc cccgattttc
    11101 gttttaccac gacgcttgaa accaaaggca atgtggaaaa cggcgtactt aagggtgact
    11161 tagtggcgcg atttggtgcc gatccgacgt taaaacgtca ggatattcgc aatatggtcg
    11221 cgactttgaa aaaatctggc gtcaaccaaa tcgatggcaa tgtgttgata gatacctcca
    11281 ttttcgccag ccacgataaa gcccccggct ggccatggaa tgacatgaca caatgcttta
    11341 gcgctccgcc tgccgccgcc atagttgacc gcaactgttt ctccgtctcg ctctacagtg
    11401 cccaaaaacc cggtgatatg gcctttatac gcgtggcatc ttattacccc gttacgatgt
    11461 tcagccaggt acgcaccctc ccccgtggtt ctgccgaagc gcaatactgc gaactggatg
    11521 tcgtaccagg cgacctgaac cgctttacgc tgacgggatg cctgccacaa cgttctgagc
    11581 cgctcccgtt ggcttttgcc gtgcaggatg gagccagtta tgccggtgca attctgaaag
    11641 atgagttaaa acaggcgggt atcacctgga gcggaacact gctgcgccag actcaggtta
    11701 acgaacctgg aacggtagtt gccagtaaac agtcggcccc gctgcacgat ctgcttaaga
    11761 ttatgctgaa aaagtcggat aacatgatcg ccgatacggt tttccgcatg ataggccatg
    11821 cgcgcttcaa tgtacctggt acatggcggg ccgggtcgga cgctgtgcgt cagatcctgc
    11881 gccagcaagc cggtgtcgat attggaaaca ccattattgc cgatggttca gggctttcgc
    11941 ggcataacct gattgccccc gccaccatga tgcaggtgct gcaatacatt gcccaacacg
    12001 acaatgaact taactttatc tccatgctgc cgctggcggg ctatgacggc tctttgcagt
    12061 accgtgcagg tctgcatcag gcgggcgtgg atggaaaagt ctcagcgaaa accggttcgt
    12121 tgcagggggt atataacctg gcgggattca ttaccacagc gagcgggcaa cgaatggcgt
    12181 ttgtgcaata tctttctggc tatgcagtag aacctgcgga tcagcgtaat cgccgtattc
    12241 cgttagtgcg ttttgaaagc cgtttgtata aagatattta tcagaacaat tagtcaaaaa
    12301 gaaaccccgg cacatggccg gggcttcaga ttattgacaa agtgcgcttt gtttatgccg
    12361 gatgcggcgt aaacgcctta tccggcctac aaaatcgtgc aaattcaaca tattgcaatt
    12421 ctcttgtagg cctgataagc gtagcgcatc aggctgattt ggcgtttatc atcagtgatt
    12481 aacgcttgta aatgaactca acgccttctt cgtcgtcttc gtcccagtcg tcatcccagt
    12541 cttcatcatc ctcttcagca atctcttcaa gctgctggcg atgataatcg tcccacatga
    12601 attcgacttt ctctggctgt ttcgcttctt cagcctgcac gaccgggttt tcaaggataa
    12661 aggtcatcac atcccagcag agatctttca cgcccagtcc gctcgccgca gagatcagat
    12721 aatatttatc ttcccagccc agcgcttcag caatcgcttt cgctttctct tcagcttctg
    12781 ccttatccag cagatcgatc ttattgaaca ctaaccaacg cggtttcgcc gccagatcct
    12841 ggctgtattt ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga tcggtgccgt
    12901 caatcggatc gatatcgatg aggtgcaaca ggacgcggca acgttccagg tgcttcagga
    12961 agcggatacc cagacctgcg ccttccgcag cgccttcaat cagtcctgga atatcggcaa
    13021 caacgaagct cttttcgttg tccattcgta ccacacccag acttggcacc agagtggtaa
    13081 acggataatc cgccactttc ggtttagccg ccgataccgc gcgaataaag gtcgatttac
    13141 ccgcgtttgg catccccaac ataccgacgt cagccagcag catcagctcc agcagcagct
    13201 cacgcttatc acccggcgta ccgttggttt tctgccgtgg tgtacggtta acggacgatt
    13261 tgaaacgggt attgcccaga ccgtgccagc cgcccttagc aaccagcaga cgctgaccgt
    13321 gtttagtcat atcgcccatg gtttcaccag taccctggtc gattacacgc gtacctaccg
    13381 gcactttaat cgtcacgtct ttaccgcgct taccggtaca atcgcggctt gcgccgttct
    13441 gaccgcgctc tgcacggaaa gatttttcaa aacgataatc gataagcgtg ttcaggtttt
    13501 cgtcggcttc catccatacg tcaccaccat caccaccgtc gccgccatcc gggccgcctt
    13561 tcggaatata cttttcgcgg cggaagctca cgcaaccatt accgccatca cctgcaacga
    13621 ccagaatcga tgcttcatca acaaacttca ttttattctc cataaatcat tcgcctgcgc
    13681 ggggttgcga taccaccgtt gtatgcttac gtaatccgcc ccaaatacga tgaccaatgg
    13741 cggaatacat cgcgcccgca accacgacaa acgcaccgag ataacctaaa aggtttaaca
    13801 tcggtctggc gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa aacagcgtaa
    13861 acagtggggt gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga gccatcgctt
    13921 ccgccagggc gccatatcct accaatgtat tcagtccgca aaaaattaaa catgcgagct
    13981 gccagtggct aagctgcgct atcactccag gctttgccag agggaagagc gcaattgtac
    14041 ataaagtgta cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat aaaacctttt
    14101 gcgccacgcc ataactcacc caaaccgtcg ccgcaccgac cccaaagata actccccagg
    14161 tgtaatcggt gagctttgta aatatctcga ccagactggt gttaaaaaac atcaccaggc
    14221 cgctcaggag catcaatgcc cctacaacct gagtgctgcg cattttctct ttcaggataa
    14281 atacgctggc aaccatcatg ccaactggcg agagttgccc aatcacctgc gaagcggtcg
    14341 gactcaggta ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc ccggcggtcg
    14401 ccactgccaa caaaatcaac cagcgtggct tacgaaacac gcgtaatggc ggcaacctct
    14461 tcttcaccgc aagaatggca ccaaggccaa tactcgccat caagaaacgg taaaacacga
    14521 ttgtcggagg ttccatcacc tccagcacct gcttcattgc gattggcaac gccccccagc
    14581 aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc ataccgtatt
    14641 ccctgcaaag cgcatttgcg gattaacgtc gcaccatgcg catttttacc ggttatcgaa
    14701 tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt taccgggacg cgaaaaactt
    14761 attcagcttc gatgctgata aatttacggt ttttcgggcc tttaacttcg aatttcactt
    14821 taccgtctgc tttagcaaac agagtgtggt cacgaccgca acctacgtta gcgccagcgt
    14881 ggaatttggt accacgttga cgaacgatga tgctacccgc cagaacggat tcgccaccga
    14941 aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg gagccgccag
    15001 cctttttatg tgccatttga aatctctcct caggtcttag gcgctgatgc cagtaatttt
    15061 cacatcagtg aaccactgac gatgaccctg ctgcttacga tagtgtttac gacgacgaaa
    15121 cttaacgatt ttaactttct cgccacgacc gtgagcaaca acttcagctt tgattacgcc
    15181 gccatcaacg aaaggaacgc cgattttgac ttcttcaccg tttgcgatca tcagcacttc
    15241 agcgaactca acagtttcgc cagttgcgat gtccagcttt tccaggcgaa cggtctgacc
    15301 ttcgcttact cggtgttgtt taccaccact ttggaaaacc gcgtacatat aaaactccgc
    15361 ttccgcgcac accttttcaa tgattcagag tgcgctataa atattcacaa tagggcgcga
    15421 atattacgca aaacgcacgc ctttgacaag tgctacagtc aatacacgaa gaaaaaaaac
    15481 acaacttgta cggtaacgtt tatctgtgcc attttttcag tacaatcacc ctatattcct
    15541 aaccataaac cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa aagcccggct
    15601 tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc aagatatggc gggtgttaat
    15661 gcggcaatcc ttgagcagct taattccgac gtccaactga tcaatcagtt aggctattac
    15721 atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg ctgtactggc tgcacgagct
    15781 gttggctatg agggaaatgc gcatgtcacc attgctgccc tgatcgagtt tatccacacg
    15841 gcgactctgc tacacgacga cgttgtggat gaatcagata tgcgcagggg taaagctacc
    15901 gccaacgccg catttggcaa tgccgccagc gtgctggtag gcgattttat ttatacccgc
    15961 gcgttccaga tgatgaccag cctcggttca ctcaaagtgc tggaagtcat gtcagaagct
    16021 gtaaacgtca tcgcagaagg tgaagttctg caactgatga acgttaacga tccggacatc
    16081 actgaagaaa actacatgcg cgttatctat agcaaaaccg cgcgtctgtt tgaggctgcc
    16141 gcgcagtgtt ccgggattct ggctggctgt acgccggagg aggagaaagg cctgcaggat
    16201 tatgggcgct atctcggcac tgctttccag ttgatcgacg atttactcga ttacaatgcc
    16261 gatggcgaac agttaggtaa aaatgtcggc gacgatctga acgaaggtaa accgacgctg
    16321 ccgctgctgc atgcgatgca tcatggcaca ccagaacagg cacagatgat ccgtaccgcc
    16381 atcgaacagg gtaacggtcg ccatcttctg gaaccggttc tggaagcaat gaacgcttgt
    16441 ggatctcttg aatggacgcg tcagcgtgcc gaggaagaag cagacaaagc catcgcagca
    16501 ttacaggtgc tcccggacac cccttggcga gaagcactca tcggcctcgc gcacatcgct
    16561 gttcaacgcg atcgttaatc ccctcccttc atcccgcgca gtgcgcggga tgattccagt
    16621 aaattccata aaaacactaa ttcagcgcta taacacctga aaaaaaaggg cgatgaatct
    16681 ttatatattc tgaatattca cactctttac aggaactttt tagagcaata ggccatcagg
    16741 agtatagtga tgcccgacag aagaagtgtt ctgaatgaaa gcgactaact taaggagtga
    16801 ggaaagatgg aaagtaattt cattgactgg catcccgctg acatcattgc gggtttgcgc
    16861 aagaagggaa cgtcaatggc ggcggagtct cgcagaaatg gtttgagttc ctcaacgctg
    16921 gcaaacgcat tatcgcgccc atggccgaaa ggagagatga ttattgcgaa agccctggga
    16981 actgacccct gggttatctg gccatcacgc taccatgatc cgcagaccca tgagtttatc
    17041 gacagaacgc agttgatgcg tagctacact aaaccgaaaa aatgagtggt ctggcggtag
    17101 ccccgcgaac ggggctgcca gctctcagac gattactcgc ctttcacacg ctcaatattt
    17161 gcacctaaag cgcgcagttt gtcttcaatg cgttcgtagc cacgatcgat gtgataaata
    17221 cgatcaacca ccgtcgtccc ttccgcaata cagccagcca gcaccaggct tgctgatgca
    17281 cgcagatcgg ttgccataac ctgtgcgcca gaaagttttt caacaccgtg acaaataacg
    17341 gtattgcttt cgatttcggc atgcgcgccc atacggctaa gctccggcac atgcataaag
    17401 cggttttcaa agaccgtttc ggtgatgaat ccggtccctt ctgccaccag gttcaacagc
    17461 gtgaactggg cctgcatatc ggtcgggaat gccggatgcg gcgcggtacg tacgttaaca
    17521 gccttcggac gtttgccatg catatccagg ctaatccagt cttcgccgac ttcgatgtcc
    17581 gctccagcgt cacgcagttt cgccagcacg gcgtcgagag tatctggctg cgcgttacgg
    17641 cagataattt tgccgcgaga aatcgccgcc gccaccagga aagtaccggt ttcgatacga
    17701 tccggtagca cgcgatagac accgccgcct aaacgttcca caccttcgat gacgatacga
    17761 tcggtgccct gaccgctaat tttcgcaccc agcgtaatca ggaagttcgc ggtatcgacg
    17821 atttccggtt cacgcgctgc attttcaata atcgtggtac cttccgccag ggttgcagca
    17881 cacatgatgg tcaccgttgc gccaacgctg actttatcca tcacgatatg tgcgcctttc
    17941 agacgaccat cgacggaagc tttaacgtaa ccttcttcca gtttgatggt cgcgcctaat
    18001 tgttcgaggc cagaaatgtg tagatcaacc ggacgcgcac cgatcgtaca accgccaggc
    18061 agtgaaactt gcccctgacc aaagcgcgct accagcggcc ccagtgccca gatagaagca
    18121 cgcatggttt taaccagatc gtaaggtgcg cagaatacat taacgtcgcg ggcatcaata
    18181 tgcacagaac cattacgttc tactttcgca cccagctggc ttagcagctt cattgatgta
    18241 tcgacgtctt tcagttttgg gacgttctgg atctctaccg gttcttccgc cagtagagcg
    18301 gcaaaaagga taggcagagc agcattttta gcgccggaaa ttgtgacttc gccctggagc
    18361 ttcgttggcc cctgaacacg aaatttatcc atttagtttg ttctcagtta acaattcata
    18421 tccgctaccg gcgaatcgcc catagctcaa aagccgttca gtttgcgatc gcgcgcccac
    18481 tccgcagggg tatacgcttt gatcgacaca gcatgaatgc ggttatccgc aatatattcc
    18541 atcagcgggc catagaccgt ctgctgtttt ttaacccgac tcatgccgtc aaacaactca
    18601 cccacggcaa taacctgaaa gtggctgcca tcgccggaaa cgtggacttc ctggagggag
    18661 agagcgttca tcaacacgct ctgaatttca ttattttcca tgggatcttc aatcatcagt
    18721 taataaacca gcgaaacatc ttagagcaaa gttgcgctgg cataaataag caaaaagcct
    18781 cgctgataaa tcagacaagg ctcgacttgc aggcaggttt gccggacagg cggttaacgc
    18841 catatccggc ctgaaaaaat ttaacgaggc agaacatcag caggcaaatt atacaatttc
    18901 gcgagggtat acactttgtc gtttaccccc tgaagcgtca cgttgttgcc ctgctttttc
    18961 gccagatcga taagatggag cagcagtgcc agtccacccg tatccacgcg ggacacacgg
    19021 ctaagatcga tgcaggtaat ccccttcacc gcttcctcac gcatttccca aagcggtagc
    19081 agaacgtcct gatccagctc tccggataac gccagcgtgt cacccgtctg catccagctc
    19141 agtgactcgc tcattatttt ttctcttcca gagtgatttt ctgttgagaa atcgatttca
    19201 gttgcgcagt caggccgtcg atacctttgg tacgcagcag cgttccccac tcgttttgtt
    19261 tggtggtgat catactgacg ccttcagcaa tcatgtcgta agcctgccag tttcccgtct
    19321 gggagttttt acgccactgg aagtccagac gcaccggcgg acggccattc gggtcaataa
    19381 tggtaacgcg aataggcaca atggttttat cgcccagcgg ctgttctggc gcaatctgat
    19441 aggtttgacc gtgatacatc gccagcgcct gaccgtaagc ctgcttcagg tactcacgga
    19501 aagcggcaaa gtaggcttca cgttgagcag gggtcgcact cttgtaatac tggcccagca
    19561 ccagcgcacc ggcgtatttc acctgtacgt atggcagcag ttcctgatca acaatggtac
    19621 gcagataatc cgggttggcc cgaatttgcg gttgctcatt cttcaggcga tcgaacgttt
    19681 tctgcgccgc ctcgtccatc agcttataag gattggtctg gtctgccgcg gttgccgcac
    19741 tcagaggtgc aatcaccagc aaagcgacca tcattaaacg tttaaacatg cgtcggttct
    19801 cctgaaatta tttcgttgta cccacaggtt cagtggtttc attattacct ggcgcagcag
    19861 ctggcgcatc gccactattc ttattgtcat cgcctttact accgtaaagg aactgaccaa
    19921 tgagatcttc cagcaccatc gcagacttag tgtcctgaat tgtatcgcca tccttcagga
    19981 tagcagtccc cagttccggg tcttcaaaac cgacgtttaa tgccagatat tgttccccca
    20041 gcaggccgga agtacgaatg ctcagcgaac tggtatctgg aatgtggtta taacgttgtt
    20101 caatttccag cgttacgcgc ggcagatagg ttttcgggtc cagcgtaata tccgccaccc
    20161 gacccacaac aacgccacca atactgaccg gagagcgcgc tttcaggccg ccaatgttat
    20221 cgaacgtcgc ataaagcgtg taggtcggtt cagtacgtat ggacgtcacg ttcgccgcct
    20281 tcaggcaaac aaacagcgcc gccagcagtg ctgctaataa aaagataccc acccaaattt
    20341 catttttttt cgtttgcatg aactcaattc ccaaacatca atgcggtcag cacaaaatcc
    20401 agccccagaa cagccagaga cgagtggaca acggtgcgag tggttgcccg gctaatcccg
    20461 gcagacgtcg ggatggtgtc gtagccgtta aacaacgaaa tccacgtcac cgtgatggcg
    20521 aacaccacgc tcttaatcag acagttgacc agatccatac gccagtcgac ggcattttgc
    20581 attgccgacc agaagaaccc gctatcaatg cctttccaac tgacgccgac cagagatccg
    20641 ccccagatcc ccacggcgac gaaaataacc gtcaacagtg gtaatgaaat aaccccagcc
    20701 cagaaacggg gagaaataac ccgacgcagc ggatccaccg ccatcatctc catactggag
    20761 agttgctctg tagcgcgcat caggccgatt tctgcggtta gcgccgaacc agcacgcccg
    20821 gcaaacaaca acgcggcaac caccggcccc agttcacgca gtagcgataa cgccaccagc
    20881 atacccagac tggtttccgc actataagtg gtcagaacca gataaccttg cagccccaac
    20941 accattccga tgaacacgcc agaaaccaca ataatcagca tcgacaggac gccgacatta
    21001 tagagctggc gcaccagcag cggcgcatgt ttgcgaaatt ccggtttgcc gaccagcgca
    21061 ttgaataaca ttaacccggc ccgcccgaac gttctcaggg ttttaatccc tttatgtccg
    21121 agcgacgcca gcgcatttaa cagcatgagt ggcttaactc cctggtaaaa gatcagcgtg
    21181 ataatcgccg gcaggatagc ggaacggaac aggcccgtca gctatcccgt ccagaaactg
    21241 acgtacgcgc ggatcaggat tcgcctgcaa cgcctgggca ctgccatgag cgacaatttt
    21301 tttgtccgcc aggatccagg cgtgatccgc aatacttaac acttccggca catcgtgaga
    21361 aaccaccaca caagtcacgc ccagcgcgct gttcagctca gaaatcagct tcaccagtac
    21421 gcccatggtg atgggatctt gcccaacaaa aggttcatca aacatgatga gatccggctc
    21481 cagcgcaatc gcacgcgcca gcgctgcacg ccgcgccatc ccaccggaaa gttcagaagg
    21541 cattagttta gccgctccac gcagccccac ggcctccagc ttcatcatca ccgtactatg
    21601 caacaatggc gcgggtagtt gggtatgttc gcgcagtgga taggcgacgt tgtcaaatac
    21661 gttcatatca gtgaacaacg ccccggactg aaataacatg ctcatccgtt tgcgcactgt
    21721 atacaggcgc gaacgagaca tcgccggaat attctcacca tcgaaaagga tctcaccatg
    21781 atctggtgcg atttgcccgc caatcagacg gagtagcgtc gttttaccga tgcccgatgg
    21841 ccccatgatc gccgtgatct tccctcgcgg cacggtcagg gaaatattat cgaagatgca
    21901 gcgattgcca cgcgtaaaac tgacatcgcg catatcgact aaattcgcca cagactgctc
    21961 cataattcac ccttcgtctt gcgttgattt tctaagcatg gcgctcaatt taaccttgaa
    22021 cccaacatat ttacagaata ttacccgccg tggttagcga aagctggcat ttgttttact
    22081 ttttagccgc ataaagtcaa aattaagaat ccgttacggc tttctgaaaa tcttcagcgg
    22141 accgacgagt atacctgaag aaaggacgtt agatgctttt agctacggca ctgttaattg
    22201 ttggtttact tttggtcgtt tacagtgccg accgcctggt ttttgccgcg tctattcttt
    22261 gccgaacctt tggcatcccg ccgctgatca tcggcatgac ggtggtcagt attggtacat
    22321 cgttaccaga aatcatcgtc tcgcttgccg cgtctctgca cgaacaacgc gatttagccg
    22381 ttggtacagc cctcggctca aacattatca atattttgct gatcctcggt ctggctgcgc
    22441 tggttcgtcc ttttaccgtc cattccgatg ttctgcgtcg tgaattaccc ttaatgttgt
    22501 tggtcagcgt agtggccggt tccgtactct atgacggaca acttagtcgc agcgatggta
    22561 tctttctcct ctttctggct gtgctatggc tgctgttcat tgttaaactt gcacgtcagg
    22621 ctgaacgtca ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg
    22681 gcggattgcc cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca
    22741 cgcggatggt ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga
    22801 cgatgggtct gacggcaatt gctatcggaa ccagcctgcc ggaactggca accgcaatag
    22861 cgggggttcg caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt
    22921 ttaatattgt catcgtgttg ggtttacccg cgctgataac gccaggagag attgagccat
    22981 tggcgtacag tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt
    23041 gctggcggcg ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta
    23101 tcgtatggct ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc
    23161 attatgtcgc acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc
    23221 ctggcgattg aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg
    23281 cttgcctgtg aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa
    23341 tcggggcaca tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt
    23401 ttcgtccatc ctggtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg
    23461 gtgattgcta tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt
    23521 aagcgtcttc acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc
    23581 gccgcagatg tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca
    23641 ccgaccagca gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta
    23701 aaagcacgcg gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt
    23761 cgtaaacttc tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt
    23821 aagaaaacgg ccagtctgcg tgacgcgttg ctggaagtta cccgcaaaaa tcttggtatg
    23881 actgtcattt gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc
    23941 cgtgtcttcg atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg
    24001 gggggaatac gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc
    24061 cgccatatca cctccgtgat ggttgccgat ggcgaccatt tactcggagt gttacatatg
    24121 catgatttac tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag
    24181 caggtgcgtc gcttgcgacc tgttacggcc ctgtcagcgc cgacgttata gcaaaagcag
    24241 agaacattcg tctgctgatc ctcgatgtcg atggcgtact gtcagatggc ctgatttata
    24301 tgggcaataa tggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt
    24361 gtgcgctcac ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag
    24421 aagatcgttg tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga
    24481 tcgcctttag cgatctgctg gaaaaactgg cgattgcccc ggaaaatgtg gcttatgtcg
    24541 gcgatgatct catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg
    24601 atgcgcatcc actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg
    24661 gcgcagtgcg cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca
    24721 aagggcaatc gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt
    24781 tctggtgatg atcggcattg atatggccga aaaagacgat accgcccagg tggtcgtcaa
    24841 caacaatgat cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg
    24901 ggcactaagc tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc
    24961 gtggtttacg cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt
    25021 aaaagcagat aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga
    25081 agtcaacgca cttgtgccaa actctcaact tcgcagaatc acgacggata acgcgcagat
    25141 caatctggtg acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt
    25201 taactccagc ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga
    25261 aaaggttaga acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg
    25321 ccagctcact tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc
    25381 cgatccacat tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta
    25441 ccggtaatgt catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta
    25501 cccgtccggg cggcgaacaa ggtaaagaag tgattgacgg ctacggtaaa ccggcaacgt
    25561 tctaccagat gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg
    25621 aactggcaaa agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca
    25681 acattaaggg cgataagatc acttacctgg taaaagagca gaaaatgcag gctttcagcg
    25741 acaaaggcaa gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca
    25801 aaggccagac cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa
    25861 agaaccttgc aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca
    25921 actccgggga aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct
    25981 acatggttgt aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata
    26041 tcagtctgct gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag
    26101 cctccatttt ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg
    26161 acgacttgtc tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca
    26221 ttgagcacct gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag
    26281 aaattgcccg cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg
    26341 gggttgaccc gatctcggtt atcgacatta aacgcatcat tgagcacctg cgcgacagcg
    26401 gcctgggcgt gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg
    26461 cttatatcgt cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag
    26521 acgaacacgt taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt
    26581 tgcgacgttt tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct
    26641 tagccaacaa ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc
    26701 gacgctggaa cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca
    26761 aatcgacact catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac
    26821 cgccgacgcg ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga
    26881 caccatttac accgctggta caccatccgg caccagcggt gactacattg acgatgagct
    26941 gccggtctat cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga
    27001 gctgacaccg ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga
    27061 tgacatcggt tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga
    27121 gatcgacatc gacgaggttg aagccgtcct taagcggatc caacggtttg atccggtcgg
    27181 tgtagcggca aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataaaac
    27241 cacgccgtgg ctggaagagg ccagactgat cattagcgat catctcgatc tgttagccaa
    27301 tcacgacttc cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc
    27361 cgtcaatctg atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc
    27421 tgagtatgtc attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact
    27481 caacagtgac agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa
    27541 cgcgcgcaac gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt
    27601 gatcaagagt ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga
    27661 acagcagcaa gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga
    27721 tatcgcccag gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata
    27781 cctgcatagt ccacgaggca tttttgaact gaagtatttc ttttccagtc acatcaatac
    27841 cgagggcggc ggcgaagctt cctccacggc gattcgtgcg ctggtgaaga aattaatcgc
    27901 ggcggaaaac ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca
    27961 aggtatcatg gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc
    28021 gtcaaaccag cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca
    28081 acattaccgg aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagccaaat
    28141 ttgccaaact tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg
    28201 agaaagtcac ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca
    28261 gcgcggaagg tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc
    28321 agttgaccaa acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg
    28381 catggcggcc tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt
    28441 ccactgtttg agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac
    28501 agcttagcag tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa
    28561 aacgcgccct ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg
    28621 tggtttttga agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta
    28681 ttgccattcc gcatggcaaa ctggaagaag atactctgcg cgccgttggc gttttcgttc
    28741 agctcgaaac gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg
    28801 ccctgctggt gtcggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga
    28861 aacgtctggc ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc
    28921 tgtatcaaat cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg
    28981 tctcttttag acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt
    29041 caggttcagg taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg
    29101 ataaccttcc cgtagtgttg ttacccgatc tggctcgaac cctggccgat cgcgagattt
    29161 ctgccgccgt cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg
    29221 cgatgagtaa cctgcctgac gctttctcac cgcaactcct gttcctggat gccgaccgta
    29281 ataccttaat tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc
    29341 tgtcgctgga aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag
    29401 cggatctgat tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta
    29461 cccgtctgct gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca
    29521 aacacggtat ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc
    29581 actgggatcc gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg
    29641 accgccacac agaagtacac aattttatct accagacgcg aagctatctt gagctatggt
    29701 tacctatgct ggaaaccaac aaccgtagct acttgacggt cgccattggt tgtaccggcg
    29761 ggaagcaccg ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta
    29821 aaaacgtcca gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac
    29881 tgttgaaatc acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt
    29941 aatgcagggt tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggccgaagc
    30001 caacagcgtt attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga
    30061 agcgaccggc ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaactctgg
    30121 ttttgatgaa gattaatctt catcactttg acatacaact cccttcaaac tccccccgac
    30181 aataagaaaa tcacgtactg aaatcgttct caatcaacgt catttgtaca ttttgtgcgc
    30241 ttttcacttt cagaagaacc ttaagaaaac cttaagaggc attgtttagg ttttgtttaa
    30301 gttaatcgac catactggag atcgtcagaa aatatttcca ggagatggca tgattcgctt
    30361 atctgaacaa agtcccctgg gcaccggaag acatcggaaa tgttatgcgc atccggaaga
    30421 tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata aagagatccg
    30481 ccgtgagtta aagtactacg cgcatcttgg tcgccggtta aaagactgga gtggaatacc
    30541 gcgctatcac ggtaccgttg agacggattg cggaaccggt tatgtctacg atgtgattgc
    30601 agattttgac ggcaaactct ccattacgct gaccgaattt gctgaacaat gccgttacga
    30661 agaagacatt gcacaactgc gccagctact gaaacagctg aagcgttatt tgcaggataa
    30721 ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc
    30781 agaggttatc ccggtcgtct gcgacaacat tggtgaaagc acattgattc cactggcgac
    30841 ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta tggaaacgat ttatcgccca
    30901 accggcgctg gctattgccc tgcagaaaga tctgcaacct cgtgagagca aaacgctggc
    30961 cctcacttcg cgcgaagctt aatccagctg gtgttgttgc ataaacggct caccgcctaa
    31021 ctgatacatc tgccgtaaaa tccacgcctg acggctacgc acgtagcccg atggcgagga
    31081 gactttgaaa cgaagtggat taggtaatac agctgccagt aatgcagctt ccgaccgggt
    31141 aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc caaacacacc
    31201 gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata cgctttttgc tccagactgt
    31261 ttctatcccc agcgttaatc cagcttccag cccttttcgt acccagctac gcccatccca
    31321 taaaaagaga tttttggccg tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc
    31381 attgcgctcg ttgtgcgcca gggctttctc aatggaagcg acatcaaagc cccagtgctc
    31441 agaaaatttc tgatcttctg cggcaatcac cgccagtccc atccacggcg agatttgatc
    31501 catactgacc cagtcagaat gtgccacgta acgaaaattg ccatgcagcc aggcgctgac
    31561 ctgtcgctcg accattaccg ctgagaaggg aacaggcgca acactaaaca acgcgatgcc
    31621 cccgccccag aaaacggcga ggacaaccat taacagcaaa agaaaacggc gaacgaaact
    31681 aaacaccgtt aagcggcttt tactcattca gccagaacca gcacgcggga aaccagctta
    31741 tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca
    31801 atcttattgt cttcatcaac cacgatatca tcgacaggac agggcacatg ctccgcgccc
    31861 atctcttcca gcacttctgc ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa
    31921 tcgaaaattt tcggcagcat cgccggggca atacacataa aaccaagcgg ttttccggcc
    31981 tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa cggtgcattc gctaccaaga
    32041 gtggcaaaat tgcttaaatt cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc
    32101 agttcagcgg catcggcctg ggccagagga cggatttcac cgcgcgttat tcgtgccgct
    32161 tcaatcagca cgttgcgcgt ttccgtcatc gcttcgccag ttaaatggtt gataacatca
    32221 acctgctgct tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga aatagctaac
    32281 aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc gctcagaatt
    32341 acgccaattt tcttcattgt gatcatcctt tcgcaattga ctgaaacaca ttaatttttt
    32401 taataaaaat ggtacgcatc acacatttaa ctgattcatg taacaaatca tttaagtttt
    32461 gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact catcataata
    32521 atttacggcg cagccaagat ttccctggtg ttggcgcagt attcgcgcac cccggtctag
    32581 ccggggtcat tttttagtgg cttttgccac ccacgctttc agcacttcta cgtcgtgacg
    32641 ccactcttct ttcatctctt caatccattc accgacgtta tcttcccagg caggaaggtc
    32701 aggagactga atttgctgac ccagctgttg cagatggcgt aaccccactg accccgccgc
    32761 acctttaatt ttatgtcctt cctcaacaat gcctttttta tcctgcgccg tcagattcga
    32821 ctccagcacg ctgacatagc ccggcatcat cttctcaaac accgctaacc cgtcggtgat
    32881 cagcttcggt cctacaagtt cgagatactg ttccagcatg ggaatatcga gcaatgcttc
    32941 tgatttactg ttctcttctg tcgtcaccgt actctcctca tcatcctggg tatcccagaa
    33001 tttcttgatc atcgcggtta gcgccggaac agaaagcggc ttactcagca catcatccat
    33061 tccagcattg aggtactctt gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg
    33121 cggtaaatcc tctcgcggat aacgcttcgt cagttcacga gagatatcca gcccggtcat
    33181 atctggcaac tgaatatcca gtaacaccag gtcgtattcg cccggtttaa acatctctag
    33241 cgccgccttg ccggtcatgg cgacatcaac gctgttacct aatttttcca gcacagaacg
    33301 cgcaacaatc acgttcagtt caatgtcttc caccagcagc acattcagcg ccggtaaagg
    33361 catatcgtct tcatcaaacg catcatcgac ctcttctgct accgacggtg cgtggatcgt
    33421 caacgtaaag gttgaacctt tgccctgttc gctggtaacc gtaatatcgc cgcccatatt
    33481 tttcgccaga cgacgagaaa cggccagacc aataccggtg ccggtggcag gtttaccgcc
    33541 atgactgtct ttcacctggt aatacatggc gaaaatttta tccagctcat cctgcggaat
    33601 gccgatacca gagtcttcca cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg
    33661 cacggtaacc tggccttgct gggtgaattt gacggcgtta ctgatgaggt tccacaggat
    33721 ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg tcggctccag
    33781 gttaaagcgc aatccttttt gttgcgcctg caatgcggag agattttcca gatcggcaag
    33841 gaagctggtg aaatcaaccg gttgattatc aagctggacc ttgcgccgtt ccatcttatc
    33901 catgtcgata atatcgttaa agatattccc cagcgtgacg gccgaaacat ggatggtctt
    33961 gagatatttt tcctgctcgg cggtgagttc ggtatccagc agaatgcggc tcagaccgac
    34021 gataccgttc agcggtgtac gcaattcgtg actgatggtg gagataaacg tcgttttgtc
    34081 gcggctggcc cgttcaagcg catcctgata ccgcttacgc tcggtaatgt ctcgaccaaa
    34141 gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac ggatttcaaa
    34201 gcaggctttg cgcccgtccg ggtaatccag ccactgttca taggtcagtg acacattatg
    34261 acggaacact ttttcatcgg tttcaatgac ttttgcggcg gcttccggtg agtaaacatc
    34321 aacaggtttc aggtgaacca gttgtttttc gctttttccg gtcagcagct ccatcgcgcg
    34381 gttacagccg gaaaactctt tatcttcgtt acgataaaaa accaggtcgg gtgaagcatc
    34441 aaggaaggaa cgtaagaatg aggattgctg ctcgagctga atttgtgtct cttcgcgctc
    34501 tttgatttca attttcagtt ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg
    34561 aacggcgatt tcctgattta gctgggcaat attatctttt aactgaacgt tgaggctcaa
    34621 atcgcgctcg cgcatctcct ccagtttttg caccagccgt gacagacgtt gtcgtgactc
    34681 ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa tcaacaaacc
    34741 aaagaagata gaacgaataa catcaatgct ttcgacctga ccatgcagca ccatggttac
    34801 cgccatttgt accacaatgg caagaacgac gagggccagc gccagcaaca ttgagaagcg
    34861 caccagacct aacttcatca tcaggtcaac ataatactgc gccagcagac gaatttgctt
    34921 cattagggaa ttccttcacg acaacctgcc ccaataatac ccaattctga cagttacgtt
    34981 ataaattgtg tgagaaatgc ggaattcccc tcaacgacct actccgttgg aggtagccac
    35041 ggacgtccca gcgccgatcc ctgtacgcca tgttcattca gatagcgatc cagctcgacc
    35101 attcccgtcc atcgattttc gcaccacaga ggagcaagca gcgtcgggcg acgggcgctg
    35161 gcggaaatgc ggtggtagat cacttccggt ggcgtatggc gaatcatttc tccggcagtg
    35221 agcgtgtaat cctccagttc aataccgttc aaacgccccg cttcccaggc tttcgccata
    35281 atgctgcctt tcacaatatg caacggatgt agctttatac cgtcaacacc ggtttccact
    35341 acccgctcca gcgtttgcag acattcagcc agcccttcgc ctggcagacc gacaattaaa
    35401 tgcgagcaca ccttcagccc gcgctggcgt gccagctgcg tcgtgcgctg ataacaggcg
    35461 aaatcatgac cgcgattaat gcgatgtagc gttttgtcgt gcacggtttg cagccccagc
    35521 tccagccaca cttcgtagcc ctggtcttta tattcgcaaa gcagatccag caccgcatcc
    35581 ggcacgcagt ccgggcgcgt gccgacacac aaaccgacaa tattggcctg gctcaccgcc
    35641 tgttgataca tcgaacgcag cacctgaact tcggcaaagg tgctggtata cgcctggaaa
    35701 taggcgaggt aacgtttcgc gcgattaatt aaattcgcct gatgcgccag ctgttcggca
    35761 atggaacgat gctgctgcgc ttcgtcggca aacgaggcaa cattacagaa ggtgcagccg
    35821 ccgcgcccga tggtgccgtc gcgattcggg caactgaaac cgccgtgcag cgtcagctta
    35881 tgcacctttt gcccataacg acgggtgaga tcaccaccaa acatattgac taatttctgt
    35941 aactgcataa tctgatagac cgcgccttga aaagaggcca aagcctgcca tttttagctc
    36001 aattcggcga tgacctggat caatcgtccc ggcccgcttt tatcaactgc ataatcaatc
    36061 aaaattaccg aaatttcatg cataatcaca taaatcactt ttgcttatct tgtgtcagat
    36121 ttttttatct ccttatggat tttaggcaaa aacagtagca tgaaacgtca ttaccaatta
    36181 atgcagtata aaatgctggt tttctcgtca attcaaggca ggataagggt taacacgcct
    36241 ttatgacagt caggaattga ctgtttctct aacgacttcc cttttagcct taaagataaa
    36301 atctatttta atttcagcca tttaataaag aattttgcgc taaagcacat ttctgtacca
    36361 ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct
    36421 ttctggatga gcagcctgct catcatattt atgcagtaat tgagatcccc tcttcaccgt
    36481 attaaccgat gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa
    36541 cccccgtcgc cacgctcttt ctgtacccgt gcgcagcggt tcggtagtgg ggttcccgca
    36601 gagcctgggg gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg
    36661 tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat
    36721 acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggtga
    36781 cggttgcggc ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg
    36841 cggctggcgt ttagcgaaaa actacgccgt cgggatgcta ttcctgaata aagatcctga
    36901 actcgccgct gccgcacgcc gcatcgttga agaagaactg caacgcgaaa ccttgtcgat
    36961 tgtgggctgg cgtgatgtcc ccactaacga aggcgtactg ggtgaaatcg ccctctcctc
    37021 tctgccccgc attgagcaaa tttttgtgaa cgccccggca ggctggcgtc cgcgcgatat
    37081 ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga
    37141 cttctacgtc tgtagcctat cgaatctggt gaacatctat aaaggtctgt gtatgccggc
    37201 ggatctgccg cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg ccatttgcct
    37261 gttccaccag cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc aaccgttccg
    37321 ctatctggcg cataacggtg aaatcaacac catcactggt aaccgccaat gggcgcgtgc
    37381 gcgtacctat aaattccaga cgccgcttat ccctgatctg cacgacgccg caccgttcgt
    37441 caacgaaacc ggctctgact ccagttcgat ggataacatg ctggaactcc tgctggcagg
    37501 cgggatggat atcatccgcg ccatgcgtct gttagtgcca cccgcctggc agaacaaccc
    37561 ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg
    37621 ggatggcccg gcgggcatcg tgatgtccga cggtcgtttt gccgcctgta acctcgaccg
    37681 taacggtctg cgtccggcgc gctacgtcat caccaaagat aagctcatca cctgcgcctc
    37741 tgaagtcggt atctgggatt accagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc
    37801 aggcgaactg atggttatcg acacccgcag tgggcatatt ctgcactcgg cagaaaccga
    37861 tgacgatctg aaaagccgcc atccatataa agagtggatg gagaaaaacg tccgccgact
    37921 ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac
    37981 gcttgccagc taccagaaac agtttaacta cagcgcggaa gagctggact ccgtaattcg
    38041 cgtactgggc gaaaacggtc aggaagcggt cggttcgatg ggcgatgata ccccattcgc
    38101 cgtgctctcc agtcagccgc gcattattta cgactacttc cgccagcagt ttgcccaggt
    38161 gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat
    38221 cggtcgtgaa atgaacgtct tttgcgaagc agagggccag gcgcaccgtt taagctttaa
    38281 atcgccgatt ctgctctact ccgatttcaa acagctcacg acgatgaaag aggagcacta
    38341 ccgcgcagat acgctggata tcacctttga cgtcactaaa accacgctcg aagcgacagt
    38401 caaagagctg tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgc tgctggtgct
    38461 ctccgaccgg aatatcgcta aagatcgcct gccggttcca gccccgatgg cggttggcgc
    38521 gatccagacc cgtctggtcg atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac
    38581 cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg ggcttcggcg cgacggctat
    38641 ttatccatac cttgcctatg aaacgctggg ccgcctggta gacacccatg cgattgccaa
    38701 agattatcgt accgtgatgc tcaactaccg taacggcatc aacaaaggct tgtacaaaat
    38761 catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc
    38821 ggtcggtcta cacgatgatg tagtgggcct gtgcttccag ggggcggtca gccgcattgg
    38881 tggggcaagc tttgaagact tccagcagga tctgctgaat ctgtcgaaac gtgcctggct
    38941 ggcgcgtaag cccatcagcc agggcggcct gctgaaatac gtccacggcg gcgaatacca
    39001 cgcctacaac ccggacgtgg tgcgcacgct gcaacaagcg gtacaaagcg gcgagtacag
    39061 cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct
    39121 gctggcaatt acgccgggtg aaaacgcggt caacattgct gatgttgaac cggcaagcga
    39181 actgtttaaa cgcttcgata ccgctgcgat gtctatcggc gcgttaagcc cggaagccca
    39241 cgaggcgctg gcggaagcga tgaacagcat cggcggtaac tcaaactccg gtgaaggcgg
    39301 tgaagatccg gcgcgctacg gcaccaacaa agtgtcgcgc atcaagcagg tggcttccgg
    39361 tcgctttggg gttactccgg cgtatctggt caatgccgac gtcattcaga ttaaagtcgc
    39421 ccagggcgcg aagccgggcg aaggcggtca gttgccgggt gataaagtca ctccttacat
    39481 cgccaaactg cgctattcgg tgcccggagt gacgctgatc tccccgccgc cgcaccacga
    39541 tatctactct atcgaggact tagcgcagct cattttcgac ctcaagcagg ttaacccgaa
    39601 agcgatgatc tccgtgaagc tggtttccga accgggcgta ggcaccatcg cgactggcgt
    39661 ggcaaaagct tatgccgatt taatcaccat cgcaggctat gacggtggca ccggcgcaag
    39721 cccgctgtcg tcagtgaaat acgcaggttg cccgtgggag ctggggcttg ttgaaaccca
    39781 gcaggcgctg gttgccaatg gtctgcgtca taagatccgt ttgcaggtcg atggcggcct
    39841 gaaaaccggt gttgatatca tcaaagcggc gattctcggc gcagaaagct tcggcttcgg
    39901 cactggccca atggtcgcgc tcggctgtaa atatctgcgt atttgccacc tgaacaactg
    39961 cgcaacgggt gtggcaactc aggatgacaa actgcgtaag aatcactatc acggcctgcc
    40021 gttcaaggtg acgaattact ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca
    40081 gctgggggta acgcggctgg tggatctgat tggtcgcacc gacctgctga aagagctgga
    40141 cggtttcacc gccaaacagc agaaactggc gctgtcgaag ctgctggaga ccgccgaacc
    40201 gcatccaggt aaggcactct actgcaccga aaacaacccg ccgtttgata acggcctgct
    40261 gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt
    40321 ctggttcgat atccgcaata ccgatcgctc cgtgggcgcg tcgctttcag gctatatcgc
    40381 ccagacgcac ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac
    40441 cgcaggccag agtttcggtg tatggaacgc gggcggcgtg gaactgtacc tgaccgggga
    40501 tgccaacgac tatgtcggta aaggcatggc gggcggctta atcgccattc gtcctccggt
    40561 tggttccgcc ttccgcagcc atgaagcaag tattatcggc aacacctgcc tgtatggcgc
    40621 gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc
    40681 cggtgctatc accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggcgg
    40741 tattgtctgt attctgggta aaaccggcgt taacttcggt gcgggcatga ccggcggctt
    40801 cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg aactggtcga
    40861 ggtcttaagc gttgacgatc tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga
    40921 gcatgttcag catactggct ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt
    40981 cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca
    41041 tcgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag caacaatgag
    41101 tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa
    41161 gatccgcaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc
    41221 gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc gagtggaaat gtccggtaca
    41281 caactacatc ccgaactggc tgaagctcgc caacgagggg cgtatttttg aagcggcgga
    41341 actgtcgcac cagaccaaca ccctgccgga agtttgcgga cgagtctgcc cgcaagaccg
    41401 tctgtgcgaa ggttcctgca ctctgaacga tgagtttggc gcggtgacca tcggcaacat
    41461 tgagcgttat atcaatgata aagcgttcga aatgggctgg cgtccggata tgtccggcgt
    41521 gaagcaaacc ggtaaaaaag tggcgattat cggcgcaggc ccggcaggtc tggcgtgtgc
    41581 ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg
    41641 cgggctgctg accttcggta ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg
    41701 ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag tgggccgcga
    41761 cgtgcagctg gacgatctgc tgagtgatta cgatgccgtc ttccttggcg tcgggactta
    41821 tcagtcaatg cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc
    41881 gttccttatc gccaacacca aacagttaat gggctttggt gaaacccgcg acgaaccgtt
    41941 cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt ggcgacactg cgatggactg
    42001 cgtgcgtacg tccgtgcgcc agggagcgaa gcacgttacc tgtgcctatc gtcgtgatga
    42061 agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt
    42121 caaattcaac gtccagccac tggggattga agtgaacggt aacggcaaag tcagcggcgt
    42181 aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat
    42241 cgttgcaggt tccgaacata tcgttccggc agatgcagtg atcatggcgt ttggtttccg
    42301 tccacacaac atggaatggc tggcaaaaca cagcgtcgag ctggattcac aaggccgcat
    42361 catcgccccg gaaggcagcg acaacgcctt ccagaccagc aacccgaaaa tctttgctgg
    42421 cggcgatatc gtccgtggtt ccgatctggt ggtaaccgct attgccgaag gtcgtaaggc
    42481 ggcagacggt attatgaact ggctggaagt ttaagctctg tcccagcgcc gcctcctcag
    42541 aaaagagtcg gcgctgaaat tctcctttct ttctggaaag cgcctcgcat aatctgaaga
    42601 aaaccaatgt aatacgctca tctggattgt taccggtaga tgcccggcgt atagctgatt
    42661 ccattattca acgcagcgcc atataaaggg acagataatg gaatcgctct cagagagaac
    42721 ctcaacaggc taccagcaaa tccacgacgg tattattcac ctggtcgata gcgcccggac
    42781 ggaaacggta cgtagcgtta acgcgctgat gaccgcgaca tactgggaaa ttggccgacg
    42841 aattgtcgaa tttgaacaag gtggcgaggc aagggctgcg tatggtgcgc agctaatcaa
    42901 gcgactatca aaggatttaa gtctaaggta taagcgtggt ttctctgcaa aaaacttacg
    42961 ccaaatgagg cttttttacc tcttttttca acatgttgaa attcgccaga cagtgtctgg
    43021 cgaattaaca ccattgggaa ttccgcagac accatctgca gaattcccat cggcaaagat
    43081 ttggcagacg ctgtctgcca aattgagagg ctgtttgata tttccacagc ttttgtattg
    43141 catctcctat tttcggtttg tatattcatg caaataaacg aaggaaagtt gccgtgattt
    43201 acattgaaca gcttgaatta atccataaaa gtggtgatgt gttgtatcca gtaaaaataa
    43261 ccagaaaatc atcaggaaaa actgccttcc accttgttcc attcggctta aataagactc
    43321 acgaccttct agaggtggaa gatgcttcag aagcaattcg gctggtgatt gatgaacgtc
    43381 attccattcg ctgcagtaca cttacagcaa ctattacgaa taaaaagggc aagcgaatta
    43441 agcggacagg tatatacagt atcaagggtg ttaacataaa agaatacaac gtcaggtaat
    43501 ggcacagagc ggtccgtcag ttcaggtttg gctctgtgcc aaagatgggt caggtcacac
    43561 ctgatctaat aaatttaagc cattaaatca gccttaacct tcaccaccac ctttttaatc
    43621 tcacccggct cgccaacaac gcatcccggc ttatgcggct ctcctagcat aaacacggcg
    43681 aacattcccg gtttcaggat gatggcttgc tcgttttcaa tggtgctgca aagctggtaa
    43741 tcatcctcat ggtggaactc ttcacactga cgcgcagtgc ctgccatgcc aaacagaatc
    43801 cgttcttcac cgtttaataa cagctggata tcaatgtatt gctcgtgcag ctccgctttt
    43861 ttctcaacgg gcgattgagt gttaaacgtc atgacattca taaaaatatt gtcgccctgt
    43921 aattcgtaac gacccggcgc tttttcttgc ggtctggcag ctaatgccag cgttaacgcg
    43981 tcctgtaacg caggatgtaa cccagcagac ggtaatgacc gtacttcacc catcatcata
    44041 atttttctcc ctgggccaac agcgctgccc caagtaaacc tgcatcatgg cggtagtgcg
    44101 ccgccagtaa atcaacatga aataccgctg gctcctgcgc cagatacgtt tccaccaacg
    44161 ccagataccc ttctgccaga ccaacgctgc caccgaccac cacgcactgg caatcagttg
    44221 tggctttaat atcagcgatc agccttgcca gcacatgtgc ggagcggtga atcagttgct
    44281 gcgcctgctc gtcgccctgc ccggcgcgtg tgaaaatagt tctcgcatcc gcgcccatga
    44341 gttcaccttg cgctgccgct gcaataccgc gaccagaagc aatcgcttcc acgcaacctg
    44401 tgcgtccaca gccgcagact gggccgtgtg gatcggcaag cgtatgcccg atatgccccg
    44461 ccagaccgcc agggccggta agcagtttac cgccgctcac tacaccgccg ccgacgccag
    44521 tggaaacggt gataaacacc atctcggtta catcgccttc cagcgcctga tactctgccc
    44581 atgctgcggc ctgcgcgtcg ttaatggcaa tggtcggcaa attggtaagt tgttccagcg
    44641 ttttgactaa cggaaagtgt agcaatccac cgagattatg cggattcagc gccagcaaac
    44701 tgccgtcgcg aataattccg gttgaagcga tggcaacccg ctgcgcatga gcttgcaacg
    44761 gagagactaa tgcggataag gcatcacgca aggcttctgg tgtctggctg gctggcgtag
    44821 gaagttcacg acgatcgcgg atctgcccgt cagcgccaat cagcgcggcg gcaagtttag
    44881 taccgccgat atcaatcgcc agtgtggtca tagcaccgcc tttttcatcg ctgtgttgta
    44941 ccactgacaa atgtgttcaa gacgcgtaat ggcagaaccg accgtcaccg cccacgcgcc
    45001 gtggcgcatc gcatctgccg cctgagcagg cgtgttgtaa cgcccttcgg caatcacccg
    45061 acatccggct tcgctcaacg ttttcaccag cgccagatcc ggctcttctg gcgtttcagg
    45121 cgtggtatag ccagaaagcg tagtgccaat aatttcggct cccagctttt ggcatgccag
    45181 gccgtcttcc ggcgttgagc agtcggtcat cgccagtaaa ccgtgatgat gtatacgtgc
    45241 cagcagcgtt tcaacaggca ccggacgcgg gcggtcggtg ccgtcaatgg cgataatgtc
    45301 cgcgcccgcc tgcgccagcg catcgacatc ttcaatatag gccgtgatgc gtaccggaga
    45361 atcctccaga tcgcgtttca caattccaat aatcggcacg ctcaccaccg cacgcgtggc
    45421 ttgcagattt gccacacctt cgatgcgaat ggcaaccgcg cccgcctgct ctgccgctaa
    45481 tgccatggct gcgacgattt cgggtttatc gagcgggctg tcaggaatcg gctggcagga
    45541 gacaatcagg ccaccgttag cagcgatttt ttgatccagt tgtgcaagta acgacataca
    45601 tcttccctta gcgaaaggcc cggtacatag accgggcaac aggattaact tttggttttg
    45661 actaaatcgt ttttggcgct gccaaatggc acggcaccgc taaatggttt gccgtcgata
    45721 gcgtcatgag tacgcaatgc ttccgggcgc agccaacgct gaacgcggga aggcatatcc
    45781 agaccaatca gcaggatcac cacaaatgtc aggctgaacg agagcgatgc cagcgcagta
    45841 cccagatcca gacgttgagc gatcaacgcg ccgatgattg gggccagtgc gccacccaat
    45901 gcgccaacgt tgtaggtaaa gcccaggccc gctgcacgct ggtcggtatc gaaataaccg
    45961 ccaatcagtt tcggtaagat cccggcgatc ccttgtccaa gcatttgctg gaagaacagt
    46021 aacagaccga gcacccagac gtttgcgccg ccaatcgcaa ataccggaat aatcagcagc
    46081 tgcgaggcca gcaggctaca aacgtacgct ttgcgggttc ccagccagtc accgaggaag
    46141 cctcctacgc agcatcccac ggccgcgcca aagccactaa agaacagcac attggcgaca
    46201 gtatgcgggt tataagccag atcggttttc agatacgttg gcagcagcgc ctgaatcggc
    46261 catgagtaga ggaaagcaaa caacacgacc accatcagca ttacgcccgt tggccagcgt
    46321 ttgcctgtac tctgcaccat aaagctgata aagattgcgg cgcataacag cccaagaaca
    46381 gcgacgatcg cggcattttg caggttaccg gcgaagcaga accacagcgc agtagccgcc
    46441 gccagtgtca ttacgatatt ggcaatgcga tgttcaccac ggtagagaat atccaccatt
    46501 gtgcgtactg gtgctttacc tgcgtgtttc tctttccagt cttccgcttc cgggatgttt
    46561 ttacgcagcc agagagcaaa gatgattggc aaaatgccga taaagaacag cgcacgccaa
    46621 ccccagaccg gaaccaccag gctatagacc tgagcggcaa cgacggcccc cacagagaag
    46681 cctgaaatca aaaaaccact ggctttgtta cgcagatgtt ttggccagct ttcaatgaca
    46741 taggtggcgc tggaaccgta ttcacccgcc atccccatgc cgatgaccag acgagcgata
    46801 aacatggtga tgtagcctgg cgcaaagccg caggccagcg tcccggccga gaagagaacg
    46861 atgctggtga ccattgccag acgacgcccg tagcggtcac ccatagcgcc gagcatcagg
    46921 ccgccgaacc agcgagagat aaaggctgcg gagatcagac ttgccgcctg caccgtcgtc
    46981 agcccgaatt caccctgtac ttcggtgagt accagggcga ttaaaacgaa atcaaaaccg
    47041 tcaagcagat atcccaacca ggcagcggaa aatgcgcgcc attgtgcacg gttgagatgg
    47101 cgataccacg ggatattctg ggttgtagta ctcattgtga gtctcccgcg gtgggcgatg
    47161 cccacacgct ttggtatgaa aattgtaggg tacagatgcg tttatttccc ctcacccggt
    47221 aggggcgagc gaggggaaaa caactcaccc gcgctcttgc atcaactgct gggccagcgc
    47281 cttcagttct ggcagatatt tttcatctac cggtccaaac ggtttgcggc acagcggcac
    47341 agaaacgaca tccatataat gcaggacagt tttcaggccg cggaatacgc cagttttgat
    47401 cagtaaatca atgactttgt tgcactcagt ttgcagtttc tgcgcggtct ggatatcgcc
    47461 ttctttcagc gccttcacga tcccctgata tcgccagccc atgatgttgt aagtactgcc
    47521 gataccacca tcagcgcctg ccagcaagcc ggaagcgaag atttcgtcgt aaccgttata
    47581 gagcaccaga tcaggatgtt cacgacggat ctgttccatc tgatagagat cgccagaagt
    47641 ctgtttcagc gcaccgacgc caggcaacgt aaccagggtg ttgatctgat ccagggtcag
    47701 ttttacccca ctcagggctg gaatgttgta cacaaccatc ggcaaaccat ccgctgaatc
    47761 aataattgcc cgatagtgat cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt
    47821 gacggcggag acggcatcga agccataacg tttagccgat gccgcaagtt gttggctttc
    47881 ggcggtgctg acgcaaccga cgtgggcgat gagtttaatc ttacctttcg cctcttcggc
    47941 gacgatttcc agtacctgtt cacgctcgga aaggctttgt acaaaggcct cgccggtcga
    48001 accacccacg tataaaccgt cgatgccctg ctgaatattg aactgaacca ggcgacgcag
    48061 actcgcttta tccagtgctt gttgttggtc aaaaggagtc aggagtgcag ccattacgcc
    48121 acgtaaattc gttgccataa atacctctga agtgatactt gtctgataaa cgatatacct
    48181 ttatacctgt tataccagat caattaagca acaccccata cagaaagctt ataatgcgat
    48241 ctgcttcact aaagtggcat tatttctttt tgttggtggt ctgaccgaaa gcgtgccagg
    48301 tagcagagac gctgttgaga tgcgattgca acgcacgatc ggcttcgtca ggatcatgac
    48361 ggcggatcgc atcaacgatc gcaatatgct gttgatagct aacgttgtta tgctcgtgca
    48421 gagcctgatc agccacggtt ggacgtgcgg cgatcagcca gtcgagcagg gcaacgtgga
    48481 tcgccatgaa gattgggtta ccagggatct ccgccagcac gcggtggaaa tcaacgtctg
    48541 aacgaatgaa tgccgcgttg ttatccagcg actgactgtt gatttccagt gcttttgcca
    48601 gcaaatcgat ttgctcatcg gtggcatgtt cagccgcata gcgcaccaga ctggattcaa
    48661 agaacagacg taattgttcg aaatgggcaa tcccaccggg atgagaaagg aaatctttcg
    48721 ccatgccgga aaactcaccg atgatagtgt ccgcagaagg acgcgagacg cgagcgcgtt
    48781 cgccgttgtt tatttgcacc agacctttgc gttttaacgc tgccagcgct tcgcgcacag
    48841 aaggacgccc gacgttaaag aacgccatca gttcgcgttc agacggtaat tgttcacctt
    48901 cgccaaattc acgacggcgg atcatctgtt ccagctcttc ttccaccatt tcggagagtt
    48961 ttttacgcgc cagcgggcgg ctacgcaagt tgcgaccaat tgcaggtgaa gaatcttcgg
    49021 tttgcgaatc aaatgcgttc ataaggccca ttctgtaagg tcagtgtgat taacatcatc
    49081 agtgacatcc tatcacagga ttgaaagtag gggaaaatgg caggtttttc tctttgtgcc
    49141 tcatcattac cataattaac ggaataatta actattgcga aaaattaatg taacgcagat
    49201 aaacacatcc cgattgaatt attcataaga ctattcacaa gcattatgaa tattatgaat
    49261 gtgttcttac aaaataatca taagcgcata ttttttaatg aaaaatcaca tcacctacaa
    49321 ttaaaaacac gacatccgca ccataaatag acttgcaaaa aatataacat cattgttttc
    49381 aatctgccgt ttatgggatt gaccgttttc ttttgacacg gagttcaaca atgttcggca
    49441 taattatatc tgtcatcgta ttaattacga tgggctattt gatcctgaaa aactacaaac
    49501 ctcaggtggt gctggctgcc gcaggtgtct tcctgatgat gtgcggagtc tggttagggt
    49561 tcggtggtgt actcgatccc gccaaaagca gcggctactt gatcgtcgac atttataatg
    49621 aaatcctgcg catgctgtcc aaccgcattg ccggattggg gctgtcgatt atggcggtgg
    49681 gcggttatgc ccgctacatg gagcgcatag gagccagtcg cgcgatggtg agcttgctaa
    49741 gccgcccgtt aaaactcatt cgctcgccgt atattattct gtcggcaact tacgtcatcg
    49801 gccaaatcat ggcgcagttt attaccagcg cctccggtct gggtatgttg ctgatggtca
    49861 ccttatttcc gacgctggtg agtctgggag taagtcgcct ctctgcggtg gcggttatcg
    49921 caaccacgat gtccattgag tgggggattc tggaaacgaa ctccattttt gcagcccagg
    49981 tcgcgggaat gaaaattgcc acatacttct tccactacca gcttccggtc gcctcttgcg
    50041 tcattatctc ggtggcgatc tcccactttt tcgtgcaacg cgcttttgac aaaaaagata
    50101 aaaatatcaa tcacgaacag gcagagcaaa aaactctcga taatgtcccg ccgctctatt
    50161 acgccatttt acctgtgatg ccgttaatcc tgatgctcgg ctcgctgttc ctcgcccacg
    50221 tcgggctgat gaagtcagaa ctgcatctgg tggtggtgat gttactaagt ttgacggtga
    50281 cgatgtttgt tgagttcttc cgcaagcata acttgcgcga aacaatggac gatgtgcagg
    50341 cgttttttga cggcatgggt acgcagtttg ccaacgtggt aacgctggtg gtcgcgggtg
    50401 aaatatttgc gaaaggctta acgacgattg gcactgttga tgcggttatc aggggtgcgg
    50461 agcattctgg tctgggcggt attggcgtga tgattattat ggcgctagtc attgccattt
    50521 gtgccattgt gatgggctct ggcaatgcgc cgtttatgtc atttgccagt cttattccga
    50581 atatcgcagc cggactacat gtaccagcgg ttgtaatgat tatgccgatg cattttgcca
    50641 cgacgctagc gcgcgcggtt tcgccgatta ctgcggtggt gaagcggaca gcgatcccca
    50701 tggcagtcgg tttcgtggtg aatatgattg ccacaatcac gctattttat taagtcatta
    50761 aaaagacaaa acaggccgcc tgggcctgtt ttgtattact tcacaacacg taatgccggt
    50821 cgaccaccgc gtggtggctg cggaggttca tcgtcaggat gagtgtcatc atcgtgatct
    50881 ggcttgtcgc catcaataac cgacataacg gtttcgttgt ctgccgatgc ctcttcatca
    50941 ttcatgatgc tggtatcttc atcgtaggca gcttcaggct caaacatcgt gcctgcgcca
    51001 ttttcacggg cgtagatagc cagcacggca gccagcggca cagaaacctg acgcggaatg
    51061 ccaccaaagc gcgcgttaaa gcgcacctca tcattcgcca gttccagatt gccgacagca
    51121 cgcggcgcaa tgttgagtac gatttgcccg tcacgcgcat attccatagg aacctgcacg
    51181 ccagggagcg tcacatccac caccaggtgc ggcgtgagct ggttatccag caaccactca
    51241 tagaatgcac gcagcagata gggacgacgt ggtgttagct gtgacaaatc catacagatt
    51301 aactccggcc cagacgcatt tcacgttctg cttcagttaa agaagcaagg aaagagtcac
    51361 gctcaaagac gcgggtcata tagcctttca gctctttcgc acccgggccg ctgagctcga
    51421 tacccagttg cggcagacgc cacagcagcg gagcaagata gcaatcgacc aggctgaact
    51481 catcgctcag gaagtacggc ttctgaccga agaccggcgc aatcgccagc agttcttcgc
    51541 gcagttgctt acgtgcggca tctgcttcag aagctgaacc gttgatgatg gtgttcatca
    51601 gcgtgtacca gtctttttcg atgcgatgca tgtacagacg gctttcaccg cgagctaccg
    51661 ggtaaacagg catcagtggc ggatgcggga aacgctcatc cagatattcc ataatgatgc
    51721 gagattccca cagggtcagc tcacgatcca ccagggtcgg aacgctctga ttcgggttga
    51781 ggtcaatcag atcctgaggc ggattgtcct tttccacgtg ttcgatctcg aaacttacac
    51841 ctttctcagc cagcacaatg cggacctgat ggctatagat gtcagtagga ccggaaaaca
    51901 gcgtcattac cgaacgtttg ttggcagcga cagccatgaa aacctccagg tatagtcaga
    51961 atttttactg ctaccagcca ccaggtggcc agtcagaagt tgtgttaccc aataaggaac
    52021 gactctcttt gttcgaaaat caaacaaaaa atgagcaata cccgacattt gggcagaaaa
    52081 ttggatgata gtttaccaga ttttgcgacc attgtggtga gtggatgccg gaaatgggga
    52141 aaaagagatg cgctttagtc tgaaatagtt gacttagacc cttattggcg atgtggtttt
    52201 tgttttgcct gtctttcaga cggcagcaaa aagcaacttc ccatttttta cgctgattca
    52261 gaatttagcc ataaaaaaac ccgccgaagc gggttttttc gtaaattgtt ttctgccgga
    52321 gcagaagcca attaacgttt ggagaactgc ggacgacgac gtgctttacg cagaccgact
    52381 ttcttacgtt caacctgacg agcgtcacga gtaacgaagc cagctttacg cagttcagaa
    52441 cgcagggact cgtcgtattc catcagagcg cgggtgatac cgtgacggat cgcaccagcc
    52501 tgaccagaga taccaccacc tttaacggtg atgtacaggt ccagtttctc aaccatgtcg
    52561 accagttcca gcggctgacg aactaccatg cgggcagttt cacgaccgaa gtactgttcc
    52621 agagaacgtt ggttgattac gattttaccg ttgcccggtt tgatgaaaac gcgagctgcg
    52681 gaatttttgc ggcgaccagt gccgtagtat tgattttcag ccattgccta taatcccgat
    52741 tagatgtcaa gaacttgcgg ttgctgtgcc gcgtggttgt gctcgttacc cgcgtaaact
    52801 ttcagtttac ggaacatagc acgacccagc gggccttttg gcaacatgcc tttaaccgcg
    52861 atttcaatca cacgctcagg acggcgagca atcatctctt caaagctcac ttgtttgata
    52921 ccaccgatgt ggccggtgtg gtgatagtac actttgtcag tacgcttgtt gccggttaca
    52981 gcaactttgt cagcgttcag aacgatgatg taatcaccgg tatctacgtg cggagtgtat
    53041 tccgctttgt gcttaccgcg taggcgacga gccagttcag tagccagacg gcccagagtt
    53101 ttaccggtcg cgtcaacaac ataccagtcg cgttttacgg tttctggttt agctgtaaaa
    53161 gttttcatta aaagcttacc caataaatag ttacacgttg gtgaacaccc aaacgtcttc
    53221 aattgttgag gttcacacga caaagtccgg caaacctacc ccttcgaata gcctatgcca
    53281 gcacacaaaa agttttggga aaaaaacttt cttgtaacgt ggggtcgcag gattatagag
    53341 aagtcggggt caaagatcga cccctttttg tgatttgtga caggttttaa cccgccaaat
    53401 gctcgcgctt cagatactct tcgctttgca tctcttgcag acgtgacagg caacgctgga
    53461 actcaaactt cagccgatcg ccctgataaa tttcatacag cggcacttct gcactcacca
    53521 ctaatttgac atggcgctcg taaaactcat ccaccagcgc aataaagcgc cgcgcttcgc
    53581 tctccatcaa ccgcgtcata actggtacat caaacaacat gaccgtatga aagagacgtg
    53641 agagcgcaat atagtcatgc tgactgcggg cgtcgacgca cagcgtagta aaagagaccg
    53701 ccagcgtctg gttctcgacg cccattgtcg ctaatggccg atggttgatt tctaacgtcg
    53761 gtgaattttc tcgtttcccc cccgccagcg ccaaccatag tttatccatt tgcgcccggg
    53821 tttcatcgtg gagtggcgaa agccacagat gcgcctgagt gagtgtacgc agtcgataat
    53881 caacaccagc gtccacgttc attacatcac aatgctgttt aatggcatcg attgcaggca
    53941 gaaaacgcgc acgttgcaag ccatttcgat aaagttcgtc cggcggaata tttgacgtcg
    54001 ctaccagggt aataccgcgg gcgaacaagg ctttcatcag accgccaagt agcatggcat
    54061 cggtaatatc agaaacaaaa aattcgtcaa aacagagcac gtcagtttcg gctttaaagc
    54121 gatcggcaat aatttccagc ggatcggtct gcccctgtaa ggcagttagc tcttcatgca
    54181 cccgcagcat aaaacggtga aagtgcaggc gctgtttccg ctctcccggc aggctttgat
    54241 agaaaaggtc catcagccag gttttcccgc gtcctacacc gccccacata tataagccac
    54301 gcactggcgt atgctttgtg tcttcgcgtt taccccacag cttaccgacc cgcgccatta
    54361 gcccactcgt cctgggggct ggtggcgtgc tattgatgag ttcctgataa ataatttcca
    54421 ggcggctgac ggcctctttt tgaacgtcgt cgggttgatg gctgccttca ttgagcgcct
    54481 tcaggtattg cgatgttggg gtaacgcttt gcatgatctt attgttattc cttgaataat
    54541 cggtgcgccg ttgttcacgg ttgacgaaaa aaaggccgtt ctacactacg cgatatgcag
    54601 tcgggattcc acttctgtgg aattaacggt tatagtggca taatcagccg caggcatgga
    54661 gcctgaagcc aacaccctac ggaaacaaaa gacaacggga gatgttcatg acctgggaat
    54721 atgcgctaat tgggttagtc gtcggcatca ttattggtgc tgtggccatg cgttttggta
    54781 atcgtaaact acgccagcaa caggcgttgc agtacgaact ggaaaagaat aaagctgaac
    54841 tggacgagta tcgcgaagag ctggttagcc actttgcccg cagcgcggaa ttactggata
    54901 ccatggcgca cgactatcgc cagctgtatc agcacatggc gaaaagttcc agcagcctgc
    54961 tgccggaact gtctgctgaa gcaaacccgt tccgtaatcg tctggcagag tctgaagcca
    55021 gcaacgatca ggcaccggtg cagatgcctc gcgactattc tgaaggcgca tccggcctgc
    55081 tgcgtactgg cgcgaagcgc gactaattta tttttcgggc gcagccattg cgccctcctc
    55141 ttctctccct ccccgactat catttaatct ggtgtctcat tgttagccgt ctgaaaattc
    55201 aataacatca aactgttttg aatctctttt cttatcattc aggtacgaga gcaggaataa
    55261 tgaaaaaaca accccagctg ttgagtgcat tagcgttaag tgtcgggtta actctctcgg
    55321 cgtcatttca ggccgtcgcg tcgattccag gccaggttgc cgatcaggcc cctctcccca
    55381 gtctggctcc aatgctggaa aaagtgcttc cggcagtggt gagcgtacgg gtggaaggaa
    55441 cggccagtca gggacagaaa atcccggaag aattcaaaaa gttttttggt gatgatttac
    55501 cggagcaacc tgcacaaccc ttcgaaggtt taggctccgg tgtcatcatc aacgccagta
    55561 aaggctatgt gctgaccaac aaccatgtga ttaatcaggc acagaaaatc agtattcagc
    55621 tcaatgatgg gcgcgagttt gatgcaaaac tgattggtag cgatgaccag agcgatatcg
    55681 ccctgttaca aattcaaaac ccgagcaaat taacgcaaat cgctattgcc gactccgata
    55741 aattgcgcgt cggtgatttt gccgtagcgg tcggtaaccc atttggcctt gggcaaaccg
    55801 ccacctctgg cattgtttcc gcattaggac gcagcgggtt gaatcttgaa ggtctggaaa
    55861 actttatcca gacagatgct tccattaacc gcggtaactc cggcggtgca ctgttaaacc
    55921 ttaacggtga gttaattggc atcaacactg caatccttgc gcctggcggc gggagcgtcg
    55981 ggattggatt tgccatcccc agtaatatgg cgcgaacact ggcgcagcag cttatcgact
    56041 ttggtgaaat caaacgcggt ttgttaggca tcaaaggcac cgagatgagt gccgatatcg
    56101 ccaaagcctt caaccttgac gtgcagcgtg gcgcgtttgt cagcgaagtg ttgccaggtt
    56161 ctggctcggc gaaagcgggc gtcaaagcgg gcgatattat taccagcctc aacggcaaac
    56221 cgctgaatag ctttgctgag ttgcgctctc gtatcgcgac caccgagccg ggcacaaaag
    56281 tgaaacttgg cctgctgcgt aacggcaaac cactggaagt agaagtgacg ctcgatacca
    56341 gcacctcttc gtcggccagc gctgaaatga tcgcgccagc gctggaaggt gcaacgttga
    56401 gcgatggtca gctaaaagat ggcggcaaag gtattaagat cgatgaagtt gtcaaaggaa
    56461 gcccagctgc tcaggctggc ttgcaaaaag acgatgtgat cattggcgtc aaccgcgatc
    56521 gggtgaactc gattgctgaa atgcgtaaag tgctggcggc aaaatcggcc atcatcgccc
    56581 tgcaaattgt acgcggcaat gaaagcatct atctgctgat gcgttaatgt cgtaaaccgg
    56641 gcatcaggct tacgtgtgat gtccggttaa ctcgtggtat gctgctgccg ttcccttttt
    56701 taatgacgcc tccatcatgt ttgtgaagct cttacgttcc gttgcgattg gattaattgt
    56761 cggcgctatt ctgctggttg ccatgccttc gctgcgcagc cttaacccgc tttccactcc
    56821 gcaatttgac agtaccgatg agacgcctgc cagctataat ctggcggttc gccgcgccgc
    56881 gccagcggtg gttaacgttt acaaccgtgg tttgaacacc aactctcaca accagcttga
    56941 gatccgcacc ctgggatccg gtgtaatcat ggatcaacgc ggttatatca tcaccaataa
    57001 acacgtcatc aacgacgccg atcagatcat cgtcgcctta caggatggtc gtgtatttga
    57061 agcattgctg gtgggatctg actctctaac cgatctggcg gtacttaaaa ttaatgccac
    57121 tggcggttta cctaccattc caatcaatgc acgtcgcgta ccgcatattg gcgacgtggt
    57181 actggcgatc ggtaacccgt acaacctcgg gcagaccatt acccagggga ttattagtgc
    57241 cacgggtcga attggtctga acccgaccgg gcggcaaaac ttcctgcaaa ccgatgcttc
    57301 cattaaccac ggtaactctg gcggcgcgct ggtgaactcg ctgggcgaac tgatgggcat
    57361 taacacgctg tcgtttgata agagtaacga tggcgaaacg ccggaaggta tcggctttgc
    57421 gattcctttc cagttagcaa ccaaaattat ggataagctg atccgcgatg gtcgcgtgat
    57481 ccgcggctac attggcattg gcggacgtga gatcgcaccg ctgcacgcac agggcggtgg
    57541 tatagatcaa ctgcaaggga tcgtggttaa tgaagtgtca cctgacggcc cggcggcgaa
    57601 tgcgggtatt caggtcaacg atctgattat ttcggtggat aacaaaccgg ccatctctgc
    57661 tctggagacg atggatcagg tggcggaaat tcgccctggt tcggtgatcc cggttgtagt
    57721 gatgcgtgat gataagcagt taacgctgca ggtcaccatt caggaatatc cggcaaccaa
    57781 ttaagtcgag tgctcaaaac aaaaaaccgg agtctgtgc
//
LOCUS       DAJTVJ010000011        55503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000011 DAJTVJ010000000
VERSION     DAJTVJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.011"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="OO448_000637"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="OO448_000637"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6335940.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO448_000638"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO448_000638"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6335941.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO448_000639"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO448_000639"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6335942.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO448_000640"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO448_000640"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6335943.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO448_000641"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO448_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6335944.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO448_000642"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO448_000642"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6335945.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="OO448_000643"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="OO448_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6335946.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO448_000644"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO448_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6335947.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="OO448_000645"
     CDS             6537..6773
                     /locus_tag="OO448_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6335948.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="OO448_000646"
     CDS             6776..7633
                     /locus_tag="OO448_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6335949.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="OO448_000647"
     CDS             7654..9645
                     /locus_tag="OO448_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6335950.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO448_000648"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO448_000648"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6335951.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="OO448_000649"
     CDS             complement(10248..11144)
                     /locus_tag="OO448_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6335952.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="OO448_000650"
     CDS             complement(11193..11432)
                     /locus_tag="OO448_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6335953.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="OO448_000651"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="OO448_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6335954.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="OO448_000652"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="OO448_000652"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6335955.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="OO448_000653"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="OO448_000653"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6335956.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="OO448_000654"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="OO448_000654"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6335957.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="OO448_000655"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="OO448_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO448_000656"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO448_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6335958.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="OO448_000657"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="OO448_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6335959.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="OO448_000658"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="OO448_000658"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6335960.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="OO448_000659"
     CDS             complement(21570..22739)
                     /locus_tag="OO448_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6335961.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO448_000660"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO448_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6335962.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="OO448_000661"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="OO448_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6335963.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="OO448_000662"
     CDS             complement(24315..25247)
                     /locus_tag="OO448_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6335964.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPIHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="OO448_000663"
     CDS             25360..26571
                     /locus_tag="OO448_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6335965.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="OO448_000664"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="OO448_000664"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6335966.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO448_000665"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO448_000665"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6335967.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="OO448_000666"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="OO448_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6335968.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="OO448_000667"
     CDS             complement(30320..31576)
                     /locus_tag="OO448_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6335969.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="OO448_000668"
     tRNA            31884..31960
                     /locus_tag="OO448_000668"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="OO448_000669"
     tRNA            31965..32041
                     /locus_tag="OO448_000669"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32046..32122
                     /locus_tag="OO448_000670"
     tRNA            32046..32122
                     /locus_tag="OO448_000670"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32080..32082,aa:Val,seq:gac)
     gene            32230..32535
                     /locus_tag="OO448_000671"
     CDS             32230..32535
                     /locus_tag="OO448_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6335970.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32661..34265
                     /locus_tag="OO448_000672"
     CDS             32661..34265
                     /locus_tag="OO448_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6335971.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34277..35089)
                     /locus_tag="OO448_000673"
     CDS             complement(34277..35089)
                     /locus_tag="OO448_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6335972.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35093..35878)
                     /locus_tag="OO448_000674"
     CDS             complement(35093..35878)
                     /locus_tag="OO448_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6335973.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35875..36543)
                     /locus_tag="OO448_000675"
     CDS             complement(35875..36543)
                     /locus_tag="OO448_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6335974.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36607..37245)
                     /locus_tag="OO448_000676"
     CDS             complement(36607..37245)
                     /locus_tag="OO448_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6335975.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37258..39366)
                     /locus_tag="OO448_000677"
     CDS             complement(37258..39366)
                     /locus_tag="OO448_000677"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6335976.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
     gene            complement(39387..40013)
                     /locus_tag="OO448_000678"
     CDS             complement(39387..40013)
                     /locus_tag="OO448_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6335977.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40469..40678)
                     /gene="fumD"
                     /locus_tag="OO448_000679"
     CDS             complement(40469..40678)
                     /gene="fumD"
                     /locus_tag="OO448_000679"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6335978.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            41235..42647
                     /gene="pykF"
                     /locus_tag="OO448_000680"
     CDS             41235..42647
                     /gene="pykF"
                     /locus_tag="OO448_000680"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6335979.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42957..43193
                     /gene="lpp"
                     /locus_tag="OO448_000681"
     CDS             42957..43193
                     /gene="lpp"
                     /locus_tag="OO448_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6335980.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43256..44260)
                     /gene="ldtE"
                     /locus_tag="OO448_000682"
     CDS             complement(43256..44260)
                     /gene="ldtE"
                     /locus_tag="OO448_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6335981.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44409..44825)
                     /gene="sufE"
                     /locus_tag="OO448_000683"
     CDS             complement(44409..44825)
                     /gene="sufE"
                     /locus_tag="OO448_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6335982.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44838..46058)
                     /gene="sufS"
                     /locus_tag="OO448_000684"
     CDS             complement(44838..46058)
                     /gene="sufS"
                     /locus_tag="OO448_000684"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6335983.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(46055..47326)
                     /gene="sufD"
                     /locus_tag="OO448_000685"
     CDS             complement(46055..47326)
                     /gene="sufD"
                     /locus_tag="OO448_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6335984.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47301..48047)
                     /gene="sufC"
                     /locus_tag="OO448_000686"
     CDS             complement(47301..48047)
                     /gene="sufC"
                     /locus_tag="OO448_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6335985.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(48057..49544)
                     /gene="sufB"
                     /locus_tag="OO448_000687"
     CDS             complement(48057..49544)
                     /gene="sufB"
                     /locus_tag="OO448_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6335986.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49553..49921)
                     /gene="sufA"
                     /locus_tag="OO448_000688"
     CDS             complement(49553..49921)
                     /gene="sufA"
                     /locus_tag="OO448_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6335987.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50469..50657)
                     /locus_tag="OO448_000689"
     CDS             complement(50469..50657)
                     /locus_tag="OO448_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6335988.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50757..51167)
                     /gene="menI"
                     /locus_tag="OO448_000690"
     CDS             complement(50757..51167)
                     /gene="menI"
                     /locus_tag="OO448_000690"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6335989.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51164..54220)
                     /locus_tag="OO448_000691"
     CDS             complement(51164..54220)
                     /locus_tag="OO448_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6335990.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHQADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54609..>55503
                     /gene="ydiK"
                     /locus_tag="OO448_000692"
     CDS             54609..>55503
                     /gene="ydiK"
                     /locus_tag="OO448_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6335991.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPM"
BASE COUNT        13376 a        14598 c        13584 g        13945 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtattggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcgttggtt cgagtccaat tgaacgcacc
    32041 atcctgcgtc cgtagctcag ttggttagag caccaccttg acatggtggg ggtcggtggt
    32101 tcgagtccac tcggacgcac cagattttct taatctggtc ttttcctttt tccctctgtt
    32161 tattctctgt atccaatacg ttaaaagatt tacactgtct tcgtatgcgt tatcagaagg
    32221 agaatcgcta tggcaacttt gttacaactt cattttgctt ttaatggccc atttggcgac
    32281 gcaatggctg agcagcttaa accacttgct gagtcgatta atcaggaacc tggttttctg
    32341 tggaaggtat ggacagaaag tgaaaagaac cacgaagccg gtgggatcta ccttttcact
    32401 gatgaaaaaa gcgcccttgc ctatctggaa aaacatactg cccgactgaa aaatctcggc
    32461 gttgaggaag ttgtcgccaa agttttcgat gtcaatgagc cacttagtca aatcaatcag
    32521 gcaaaactcg cctgacagaa tttaatcaag ggcggttagc gcccttttca tccctgtctg
    32581 aaatttctca aattctaaaa atctcaacca aacttatctg ataacactaa attcgaaaga
    32641 atgcgtacag gtaagtaaca atgaaaaaaa ttgctattgt gggtgccggg cctacgggga
    32701 tctacacctt attctcgctt ctacagcaac aaactccact ttctatttct atcttcgagc
    32761 aggctgacga ggccggtgtc gggatgccat acagtgatga ggaaaactca aaaatgatgc
    32821 tggcaaatat tgccagtatt gaaataccgc cgattaattg tacgtatctc gaatggctac
    32881 aaaagcaaga agccagccat ctccagcgtt atggcgttaa aaaagaaacc ttgcacgatc
    32941 gtcagttttt accgcgaatt ctgctgggcg aatatttccg cgatcaattt ttacgattag
    33001 tagaccaggc acgacagcaa aaatttgcag tggctgttta tgaatcatgc caggttaccg
    33061 atctgcaaat tacaaatgct ggcgtcatgc tcgctacaaa tcaggattta cccagcgaga
    33121 cgtttgattt agcggtgatt gccacgggtc acgtctggcc tgatgaagaa gaagcaacca
    33181 gaacgtattt tcccagcccg tggtcaggcc tgatggaagc aaaggtcgat gcgtgtaacg
    33241 tgggtattat gggaacatcc ttgagcggac tggatgcggc aatggcagtg gctattcagc
    33301 atggttcgtt cattgaagat gataaacaac acgtcgtttt tcaccgcgat aacgcaagtg
    33361 aaaagctaaa tatcacgttg atgtcgcgca cgggtatttt acccgaagcc gatttctatt
    33421 gccctattcc ctacgagccc ttacacatcg tcaccgatca ggcattaaat gctgagattc
    33481 aaaaaggcga atatggcctt ttggatcggg tatttagatt gatagtagag gaaatcaagt
    33541 ttgctgatcc agactggagc caacgcatag ccttagagag cctgaatgtc gattcctttg
    33601 ctcaagcctg gtttgccgag cgcaaacaac gcgacccatt tgactgggca gaaaaaaatc
    33661 tccaggaagt cgaacgcaat aaacgagaaa aacatactgt tccctggcgt tatgtcattc
    33721 tgcgcctgca tgaagccgta caggaaattg ttccacatct gaatgaacac gaccataaac
    33781 ggttcagtaa aggccttgcc cgggttttca tcgataatta tgcggcaatc ccttcagagt
    33841 ctattcgtcg gctgctggcc ttacgtgaag cggggatcat tcatattctc gccctcggtg
    33901 aagactacaa aatggaaatt aatgagtcgc gcaccgtcct gaaaacggaa gacaacagct
    33961 actcgtttga cgtttttatt gatgcccgcg gacagcgtcc gcttaaagtg aaagatattc
    34021 ctttccctgg gctacgcgag caattacaga aaacagggga tgaaatccct gatgttggcg
    34081 aagattatac gttacagcaa cccgaagata ttcgtggacg cgtagcgttc ggcgcgttgc
    34141 cctggttgat gcacgaccag cctttcgttc agggacttac ggcatgtgca gaaattggtg
    34201 aggcgatggc tcgggcggtc gtaaagcctg catcccgtgc tcgtcggcgt ctttcgtttg
    34261 attaaaggta aagctatcag cactcaacaa actgatgctg ttcattaaga cgatagcgga
    34321 ctccggttcg aatattgttt tcaccttcaa tggcgacggc gaagcgaact ctttcgccgt
    34381 catgatatgc cagtgcggcg cttccccctg gcccgagaat aattgagtcg accacgcccg
    34441 tgctcactac cgttgaattt tcaccgctgg caatgatgtg tacattgtcg ccgctgttgg
    34501 caattctggc gtttgcgcca aagctggcga tttgcaccag gtcaccatta ctggcgacat
    34561 gacaccgttc acccaatgta caaacgcgta ctcgcattcc ggtattcgca atccgactac
    34621 tatctccagc gctgcttatc cgcgaagaat taccggttac agcaacccgt gcgcgttcac
    34681 cggagctgcc aatgtgactg ttaaagccga cacttccaat ccgcacgcta taacccatac
    34741 tggcaatttg cgcggcatac ccagcggtta cgattcgggc gttatcctca caacagccga
    34801 tgcgagcatg atcgtcgctg cgggcaaact gactgcaaaa tggtatctct ccggggtctg
    34861 tggccagttt ttgcatagag gaaacttcct gatgcgcgaa gttttcatcc gccagccatt
    34921 ttttccacgc atactcaacc agactctccg cccagtccgt atatccttcc tcaatcagtg
    34981 catgatgaat atcggcataa ctaccgccgc gtgggaaatg gcgcaggaac cagcgataca
    35041 tcaccgctcc gatacgccat tctcgcaggt ctgctcgtgt aatgatcatt cgttagtgcc
    35101 ggtgatagcc atcgaccatg cttttaaagg tttcgtgtgg cgtacgcccc gtggtgcaaa
    35161 gataaagatg tccgaagata aaaaagaggc ttataaatgc cagagcaaaa tgcgcctgca
    35221 ataaccagta tcttacgcca ggaaacacat ctcccacggc ttgcggatag agacacaaca
    35281 gccccgtcaa tagtaacaac ggcagcaatc cacacatgac accaacatag gcgacctgct
    35341 gtaagggatt aaatttagac tgtgttgttg ccgggaaagg atgttcttcc ccctgcataa
    35401 tgccaaacaa ataaaatcgt gtttgttttg ccgctcgttc cagccacccc tgacgacgaa
    35461 tgcgatagtg gtgaccatta tccccaacgg cattgatcag cacaaagccg agccagcatg
    35521 ccagtaacaa aaatccgcaa acttcatgca ccgcaaccag acttttaacc gcagttgcgc
    35581 ccaccatcgc aaagtgattt atcagcccac ttgccagcaa caatacaaag agtaacgcat
    35641 tcgaccagtg ccataatctg accgctttgc tgtaaagata aaccttctca ccatgatcgg
    35701 tggctgattt cttcacgcca cgagcacgaa gcaaggcgtg cagccccaac accaaccaca
    35761 tcccaaccag caatactccg gcaatgatca accacaccgg ccaaaattcg ggagtgaact
    35821 gtggcacata gttcgctaac tggctctgaa actgttcagc atgttgcgac gggttcatac
    35881 attttccttt ttaatcaaat gttgaccgaa ccggcgatac agatgcggtt tcccggcacc
    35941 aagtagctga tattgatagt atttattttc ctgcaaccac gcctgaattt ctgggctatc
    36001 ttcgcgacca aagatcaatg cgtgttccgg acaggcgctg acgcaaatgg gcggaaagcc
    36061 ttttgccagt cgggactcag cgcagaaatc acatttatct gccactttag tgacagggtt
    36121 aaggtagcga acctggtacg gacaagcgcc aatgcaataa ctgcagccga tgcactgcga
    36181 tttttctacc cgcacgatcc cctgctcatc acgccacgac gcccctgtcg ggcatacgtc
    36241 gatgcacgga gcatcttcgc agtgctgaca tgactggcga aaaaagtgat actgcgtttc
    36301 gttgtcgtta tcggtgactg gaatgtgtgc tatcgataaa cggcttccct gagcggggac
    36361 gtggttagtt ttacggcatg cgcgggcgca aatattacag ccattgcacc gtgactcgtc
    36421 atgaatcatg gcataccgaa cttccttttc ttgcctcgtg ttcgctaaca gagaagacgc
    36481 agaaccagta aaaaagatta ctgttcccat ccccagaaca aatttgcgtc gagtgaagga
    36541 catctgatta tcccctaatg cagtggctgc aactgtccac cctatccatg acatctccct
    36601 gaaatttcag gggttctgaa actgctctac ctcgaaccat tcgcataagc cataagcctt
    36661 gccataggtc ataaatcgct cgctaaacaa gtacaacacc gccgtcgatg actccacctg
    36721 ccgtatatcg gcatattcag gtgcaacatc catcgcggcg atcgcggctt caatctgcgc
    36781 ttcctggaag taatatggcg cttgcatcag catggcgact ttgtaaggac gaggatacgt
    36841 ctggcactca aaacgaacgg cgtgtgcaat ggcgcgacaa atgtcctgct ccacaacttg
    36901 caaagacatc gccgcgtagt tttcactcat ggcctgcgtt gagtaatagt aatcatcctg
    36961 acttcccgtc atacaagcga tatccgcgta gtctccgttc tgcttaatgc tttccagcaa
    37021 aactgacagt tcctcttctg caaccgaata gggtggcgag agaaacacgg tgcgcgccac
    37081 cagctgccca gccgcagaat gctggcgaat gaactcagcc aggatttcag caggttccgg
    37141 ttcggttacc gtatcaacgg ggtgacgcat cccctgcaac cattgccgct tcgcctcgtc
    37201 aacttcgctg actttcgcta acggcaattc atcctgatgg ttcatcttac ccattcctta
    37261 cgcaggcagt agattgtgtg ctgccagatc ggcggcaata tcttccagcc ccagccgctg
    37321 caatgtttcg cgggttggac aaccaagctg tgggtcccag cccatttctt tgtagaacat
    37381 cgtgagcgaa gcatgcatat cgtcacgatc cattttgtca gtaccttcag taaagaccgg
    37441 gatctgcgga tccttgtcga aaacccagga acagataaga tcgtgttcat tacgcatatc
    37501 tttggtttgc atcagtttta ccgtgtaggc acgatgcaac gtaaagatac gctctgcagc
    37561 taaatctaat ttttcctgag tcatctcttc gccggtgatc gctttgaaga atttggcttc
    37621 aagcgccaga tcgccccggt aattacggct tttcagcggc gaaacggtca ttggccagac
    37681 ccagttgcac agggtgacgg cgttatgcaa acagacccgc aacagcgacc actttgcata
    37741 tttaattttt gcgtcattga ttggcgtgta gtttttggtt tcatcgtaag catcttcaga
    37801 accaaaaagt tctttcgcca cttcacgttg cagtttcaat ggcaagccgg aaccaataaa
    37861 gttgatatgg gtatgcgtca tgcagtcacg gttgaacata cagttaacaa tggaaccgac
    37921 ctgcgctgac gcttcattgg catggtgaac cggatagcca aacggcgacc agagtttatt
    37981 tttcgcgtag ccccagtact cttcacccaa attccagcgt tctgcgatgg catatgaacc
    38041 atcagccagg tgactcagct cacccacacg atgcgccaga cggtagtaaa aatctttaat
    38101 tttaatgaag ttaacgtcac ccgcttccag ttgatcccag cgaatttccg catactcttc
    38161 agcaggcaga acacgcttga acacaccttt gctgtaacaa taggtaaagt cgcgatgcaa
    38221 ctgcccgtag ttacaccata ggccgtagtc atcgaacaga ttcagaccca ccaggttacc
    38281 aatcacacga ccatcgtctt tatcttcaaa atctttcgga ccgttcggga agatggtggt
    38341 atggacaaag ttggctacac aagtgttacc acctgtgctg ggcacgccaa actctttcac
    38401 ccgaggaata ttcatttggg tcatacaacg gatcgggcaa gagtggcagc cactcatttt
    38461 tactgtgtat ttctctgccg ccggacctaa gtcaaaaacg gatttatagg tacgaaagcc
    38521 gaccgtgttc tgattgcctg gcggaatttc ccccgtttca atcggaccac cttcagccgc
    38581 gccccaaaac agccctttac gtgctgtcca acgtgacttg ggatctgaat actctgccca
    38641 cgattgcggc gtacttggca cgacatggtt gttattcgca ccaataagtt cagtcatcat
    38701 gtaatcgttg agacgcttca tctcctgacg atcggcaatg ttgacccctt tcgtcccttc
    38761 aaccgcaatc gctttcaggt ttttcgaacc cattattgcg ccagttcccg caccgccgct
    38821 gtggttacgg ctattcaaca tgccagagag aggaacaagg ttttccccag cctgaccaat
    38881 agccgccaca caggtttccg gactggtcaa tcgacaaatt tcttccgtcg ttgcgcgcgt
    38941 cccttttccc cataagaaat cggctttttc caggctaact ttgtcatctt taatcttcag
    39001 ccataccggt gatttcgcct tcccttcgat aataatgacg tcgtatccag cgaatttcat
    39061 ttgcgctgca aaaaagccac ccatatgggc atcgacgact aaatttcctt tggtaaaagt
    39121 agaaagtgag gtgatattta cgcgagaact acagggggca ccagatccag ttaatgggcc
    39181 ggtagcaaag actaatttat tcgcttcatc gaaaggtttc gtgcctggcg gtacttcgtc
    39241 atacataatt ttgtagccga agcccatgcc accgacaaaa cttttaaact tactggaatc
    39301 ttcgagggta atatttcctg tcgtgagatt gactcttaat atattacctg tccaaccgtt
    39361 agccatgatt ttttcctttg caagattcac acagtaatat ctttccactc gataattttt
    39421 aacgcccctg ttgggcaggc gtttgcgcat tcaccgcata acacacattt cgaggatttt
    39481 ttactttcgg tatttacggt ggccatcatc cacgggcatg ccgtagtaca ggcgctacag
    39541 ccaatacagc gtttatgatc gacggtaata cagccttctt tctgctgcca ggtaatcgca
    39601 ccaatcgggc agacgttcat gcattgcggc tctttgcatt gacggcaggt gtccgcggta
    39661 tagttgagat cgccatacag gccgccgcca gagccaaccc cgttgtcgcc aaagaaataa
    39721 ttgcgatgga ttttgatacg ggagaagaat gtccctactg agccatcgtt gaagttggta
    39781 caagagattt cacagcggtg gcaacccgtg catcgcgctc gctgggtcac cagcacccct
    39841 ttgggtgtat ttatcaaccc tactgtgccg ctatcaatat cttcctgttt gcaacccaaa
    39901 agtgacagta acgcaggggc aattgttaga ccagcaaggc cttttcctga tatacgcaag
    39961 aattctaatc gcgttaagcc aatatctaat agtggacgat caaccgggtt catttatttc
    40021 gcatcctctt cgcaaacaca gtgctgacaa ttcagcagcc tgatattaag cattcaacta
    40081 atacgctgct gactctgtcc ccaacaccag atattttccc taacccgata gtgtatgaga
    40141 aaagatttaa cgccgataaa ccaatccttt atggttagga gaaaaaataa tgtgattatt
    40201 ctgagccctt aacattgatc gttatcaatt aaaattacaa agcaagaaat tataagtgaa
    40261 ctgatattta ttatcaattg aaataaattt aacttaagcg acaattattt gtgacgttca
    40321 ttgcagaaaa taaaaataat attatcgtta tagattaaaa tacataccgc aattcgtata
    40381 atttcagcag ttgttattta gaatgattaa ctggaccgca actgaagcaa gaatatcagg
    40441 atgaagctta ccgcctcatc ctgaatggtc attttaccag tgcgttaatg acggtttcca
    40501 tcgcctgctt ttccagctca ctgcgctgaa tgcgtttatt ggctaatgca gtacgtaata
    40561 cgcctgcgat aacaatatgc tttggttcct gccccagatc gcgcatttcc agcacaactt
    40621 taccgacaac tctgcacatt tctcggtaca gttcatcctc ttttgtccga tttcccattc
    40681 tttataccta tttatcatgc caactatcag catatatcaa tctaaccaat taaacaaaaa
    40741 tcaaacaaaa tcagacaaat aacgcgataa attattttaa ctgtagcaat tgagcgatga
    40801 tatatttata caccggatga actttcactt atcctcacac tgacaacttc ggcaccagac
    40861 gttgcgcaaa cagcgaagtt tttgcgtaac cttttccctg gaacgttaaa tctttgataa
    40921 caatttattg tctaacaagt tgtatatttt ttgaaacgct gtttttgttt tccttttgga
    40981 ttaatttcag cgtataatgc gcgccaattg actcttgaat ggtttcagca ctttggactg
    41041 tagaactcaa cgactcaaaa acaggcactc acgttgggct gagacacaag cacacattcc
    41101 tctgcacgct ttttcgatgt cacctatcct tagagcaagg caccaccact ttcgtaatac
    41161 cggattcgct ttccggcagt gcgcccagaa agcaagtttc tcccatcctt ctcaacttaa
    41221 agactaagac tgtcatgaaa aagaccaaaa ttgtttgcac catcggaccg aaaaccgaat
    41281 ctgaagagat gttagctaaa atgctggacg ctggcatgaa cgttatgcgt ctgaacttct
    41341 ctcatggtga ctatgcagaa cacggtcagc gcattcagaa tctgcgcaac gtgatgagca
    41401 aaactggtaa aaccgccgct atcctgcttg ataccaaagg tccggaaatc cgcaccatga
    41461 aactggaagg cggtaacgac gtttctctga aagcgggtca gacctttact ttcaccactg
    41521 ataaatctgt tatcggcaac agcgaaatgg ttgcggtaac gtatgaaggt ttcactactg
    41581 acctgtctgt tggcaacacc gtactggttg acgatggtct gatcggtatg gaagttactg
    41641 ccattgaagg taacaaagtt atctgtaaag tgctgaacaa cggcgacctg ggcgaaaaca
    41701 aaggtgtgaa cctgcctggc gtttccattg ccctgccagc actggctgaa aaagacaaac
    41761 aggacctgat ctttggttgc gaacaaggcg tagactttgt tgctgcttcc tttattcgta
    41821 agcgttctga cgttatcgaa atccgtgagc acctgaaagc gcacggcggc gaaaacatcc
    41881 acatcatctc caaaatcgaa aaccaggaag gcctcaacaa cttcgacgaa atcctcgaag
    41941 cctctgacgg catcatggtt gcgcgtggcg acctgggtgt agaaattccg gtagaagaag
    42001 ttatcttcgc ccagaagatg atgatcgaaa aatgtatccg tgcacgtaaa gtcgttatca
    42061 ctgcgaccca gatgctggat tccatgatca aaaacccacg cccgactcgc gcagaagccg
    42121 gtgacgttgc aaacgccatc ctcgacggta ctgacgcagt gatgctgtct ggtgaatccg
    42181 caaaaggtaa atacccgctg gaagcggttt ctatcatggc gaccatctgc gaacgtaccg
    42241 accgcgtgat gaacagccgt ctcgagttca acaatgacaa ccgtaaactg cgcattaccg
    42301 aagcggtatg ccgtggtgcc gttgaaactg ctgaaaaact ggatgctccg ctgatcgtgg
    42361 ttgctaccca gggcggtaaa tctgctcgcg cagtacgtaa atacttcccg gatgccacca
    42421 tactggcact gaccaccaac gaaaaaacgg ctcatcagtt ggtactgagc aaaggcgttg
    42481 tgccgcagct ggttaaagag atcacttcta ctgatgattt ctaccgtctg ggtaaagaac
    42541 tggctctgca gagcggtctg gcacacaaag gtgacgtcgt agttatggtt tctggtgcac
    42601 tggtaccgag cggcactact aacaccgcat ctgttcacgt cctgtaatat tgcttttgtg
    42661 aattaatttg tatatcgaag cgccctgatg ggcgcttttt tatttaatcg ataaccagaa
    42721 gcaataaaaa atcaaatcgg atttcactat ataatctcac tttatctaag atgaatccga
    42781 tggaagcatc ctgttttctc tcagtttttt tatctaaaac ccagcgttcg atgcttcttt
    42841 gagcgaacga tcaaaaataa gtgccttccc atcaaaaaaa tattctcaac ataaaaaact
    42901 ttgtgtaata cttgtaacgc tacatggaga ttaactcaat ctagagggta ttaataatga
    42961 aagctactaa actggtactg ggcgcggtaa tcctgggttc tactctgctg gcaggttgct
    43021 ccagcaacgc taaaatcgat cagctgtctt ctgacgttca gactctgaac gctaaagttg
    43081 accagctgag caacgacgtg aacgcaatgc gttccgacgt tcaggctgct aaagatgacg
    43141 cagctcgtgc taaccagcgt ctggacaaca tggctactaa ataccgcaag taatagtacc
    43201 tgtgaagtga aaaatggcgc acattgtgcg ccattttttt gtctgctatt taccactact
    43261 gcgtcgcgcg taacatattc ccttgctctg gttcaccatt ctgcgctgac tctactgaag
    43321 gcgcattgct ggctgcggga gttgctccac tgctcaccga aaccggatac cctgcccgac
    43381 gatacaacgc tttatcgact aacttctgat ctacagcctt attgtcttta aattgcgtaa
    43441 agcctgctgg cagtgtgtat ggcattgtct gaacgttttg ctgttcttct gctgatagtg
    43501 gtcgatgtac ttcaacataa cgcatcccgt taggttccac ggaatatttc accggttcgt
    43561 tgatcacttt caccggcgtt cccgtccgca cgctggagaa caaggcttta atatccggtg
    43621 cattcatgcg aatacaccct gaactgacgc gcaaaccaac gctgtccggc gcactggtgc
    43681 catgaatgag gtattcgcca ttaccatgcg cgaggcgcag tgcgtaacgt cctagcgggt
    43741 tatttggtcc ggcaggaacg actggcggta atttaatgcc acgctccagc gaacgctgac
    43801 gaatgcctgc cgtaggcgtc caggttgggt tagggatttt ctgcccaaca cgcgtttcca
    43861 tcaccggcgt ttccagcccc tgcaatccaa tacctattgg ataaacctgc acaatatttt
    43921 ctcccggcgg ataataataa aggcgcagct ctgcaaggtt aacgataatc ccctgacgcg
    43981 gtgcatcagg taataacagt tgtgaaggaa tagttatcgt cgtaccaggt tttggcaccg
    44041 ggacgatagt gttattggct tcaaggatca acattgccgc agtatcaaaa cgtcgggcaa
    44101 tagcctgaag gtttttatcc ccttcttgca ccgtatacgt ttgattttgc ccaaccagtc
    44161 ggcttccggt tggtggtagc ggataatcaa ccgcccaggc agcctggatg gcgctaaaag
    44221 cgccgataag cgtgagtgta agcaaagacg cgcgtttcat tgtaaacctc ctgtatttgc
    44281 cggagactca cgctgaaacg tcggatggcg cttatgttca cctgaaacca aaacactcct
    44341 gtgcaggtca gtgtaaacat tgaccatccg gcaatgtgag ccaaccggat gaaagctgtc
    44401 cctttagttt agctaagtgc agcggctttg gcgcgaattg cgcgaatcat cgcttccaga
    44461 ccttgtgaac gagatggggt gagatgttgg gtgagtgcca ttttttcaaa ccacggacgc
    44521 acatcgaaac tgacaatatc ctgcggcgtc atctgatcgt agagaataaa gactaccgca
    44581 ataagccctt tcacaatcgc cgcatcgctg tcgccctgca attcaataat tccgtgcgca
    44641 ttctggcgca tgacaatcca cacctgactc tggcagccct gaatgctatt ttgtgggctt
    44701 cggtcttcgt cgcgtaattc agtcagacgc tggcccagct caataatgta gagatatttc
    44761 tcttcccagt tggcgcagcg taaaaaatta cgcagcaatt tttctttatc cggcaataaa
    44821 gccatagtgc ctccctgtta tcccagcaaa cggtgaatac gttgcaggcc ggtcaccaga
    44881 cgatccactt cttcatgggt gttatacatg gccagcgacg cccgacacat cgcagggacg
    44941 ttgtaatagg ccattaatgg catagcgcag tgatgtccgg tacgcacagc aatgccgtaa
    45001 ttatcgagaa aactgccaac atcataggcg tggtgtttac cgagattaaa agcaataacg
    45061 ccaagcctgt tttgtgggcc atagagagtg agatccggta cagattccag ctgtgatagc
    45121 gcataatgca tcagattctg ttcatactcg gctatgttat taagccccag cgccgaaaca
    45181 tactccagcg ccgcgccaag accaataatg cccccggtat tgggtgtacc ggcttcaaac
    45241 cgccatggtg ctttggtcca ggtagtgcct tcactcaggc tgacggtggc gatcatagaa
    45301 ccgccccctt cccacggcgg catctcctgc aacaaggctt ctttcacata aagaatgcca
    45361 attccggtgg ggccatacag tttatgcccg gagaacacgt aaaagtcgca atccagcgcc
    45421 tgaacatcca ccggatgatg catcaccgcc tgagcgccat ccaccagcac ttttgcgcca
    45481 tgctggtgcg caagcgtgat tatttccgcc agcggatttt ctgttcccag cacgttggag
    45541 acatgagtaa ttgccagcag gcgagttttc tcatcaaaca gcgtaggcag cgtctccagt
    45601 tgcagcgtac cgtcggggtt gagagggatc acacgcagct ctgcgccaac gcgtgcgcaa
    45661 agcatctgcc agggaacaat gttagcgtgg tgctccatct gactgatgat gatgttatcg
    45721 cccgcccgca cgttgctgtt gccccaacta ttggcgacca gattgatccc ttccgtcgtg
    45781 ccgcggacga acaccagctc ttccgccgaa cgggcattaa taaacagcga tgcccgcttg
    45841 cgcacgttct ccattttctc ggtcgcctgg gcgcttaagg tatgaatacc gcgatgcacc
    45901 gccgcgtagc cgtgacgata aaactcagcc tcggcgtcaa tcacctggct tggtttctgc
    45961 gcactggcgg cgctatcgag ataagccagc ggcaaaccgt tcacctcacg agaaagcacc
    46021 ggaaagtcgg cccgcacttt gtcgacggaa aaagtcatct tgcacctcct ggcagccgtt
    46081 gaccgattcg ggccagcacc tgctgtttaa gcccctcatc acgcagtgct tccgtcagtt
    46141 cggcagcaaa ggcgtaaatg atcatctgct gggcatcctg ctgattgatc ccgcgcgagc
    46201 gcagatagaa catctgttca tcatcaatac gccccaccgt cgcgccgtgg ctgcatttca
    46261 catcatctgc atagatttcc agctgcggtt ttgtatccac ttccgccatt ttgcccatca
    46321 gcagattatt gttggtcatc tgaccatccg ttttgatggc gtgctgcgcg acgttgatca
    46381 aaccgttaaa taccgcgcgg cctttgtcgc tgacgatagt tttgtgcaac tgtcggctgt
    46441 tacaaaaacc tttattgtgt tccagccagg tacgggtatc acacacctcg tttttcaccg
    46501 gcatcgccag gctattgatc cgcagcgtgc tgttttcgcc attgagttgc gtactggtgt
    46561 tgtgtcgtaa cactgcgcca cccagcagga aactatggct aaatgcggtg gcatcctcag
    46621 ccagcaacaa atcgttatga gcaaagtggt gactgagcgg gttttcaaac gccagcttga
    46681 tatgctgcaa gtgggcgttc gctgcgacat tgatagtgaa ccgtgccccg gtaaaatgac
    46741 gagcatcatt aaggctgaca aaatgttcga tcaccgttgc ttcggcacct tccgccagat
    46801 ccagatgatg ccggtaatgg gcagtgttca cctcttcacc tgccacgccc tgggtgatat
    46861 gcattaacag caatggcttt gccggccgtt gaccgcgctt cacggcgata tgcgtcacgc
    46921 tttgtgccag gctttccgtc aaatgcagaa acacttccgc ctgaatagcg tcgggtaacc
    46981 cctgacggtc gtcgttaatg ctcacttcat atccgctgcc ttcaattgca tcgctcagcg
    47041 cgggcacgta acgtccatcg acaaacacca gccgcacggc gtctaacgtt aacgctaagg
    47101 catcacgctg ctgtggggat atatctcccg caatgctgac aaactgacta ttggtcagcc
    47161 cttccagcgg cgtatatttc cagttttcat gtttacgtgt cggcagtccg gtacgcagca
    47221 attgttgtaa atgctgctgt gcttgcggtg agcgttttgt cccttcagct tcaaacaagt
    47281 gatgccactg ttgcagcgca ttactgctgt tcggtaagcc agccataacc ctgctcctcc
    47341 agttgtttga ccaacgtgaa atcgccggat ttcacaattc gtccctgata tagcacatgg
    47401 acgtaatcag gcttgatgta gtcgagaatg cgttggtagt gcgtaacaat gatgaatgag
    47461 cgcttgccgt cacgcagcga gttcacgcca tcggcaacca cttttaatgc gtcaatatcc
    47521 agcccggagt ccgattcatc aagaatgcat aactccggtt ccagcaccgc catttgcaaa
    47581 atatcgttgc gctttttctc gccgccggaa aaaccaacgt ttaccgaacg ggttaataaa
    47641 tcttccggca tcttcaggag agcgattttc tcttccatca aatcctgaaa atcaaagcgg
    47701 tcgagcgttt cctggccgcg atagctgcgc accgcattaa gtgccgtttg caggaaaaac
    47761 tggttactga cgccgggaat ctccaccgga tactggaagg ccataaagat gccttcgcct
    47821 gcacgatctt ccggcgacag cgcaagcaaa tctttgcctt tgaactcaac cgtgccgccc
    47881 gtcacttcat aatcttctcg cccggcaagc gttgccgata aggtactttt gcccgaaccg
    47941 tttggcccca taatggcgtg aacttcgccg ggacgaacgt cgaggcttaa tccacgcagg
    48001 atagctttat cttccacgct gacgtgtaaa tctttaatac ttaacatgtt tattccttat
    48061 ccgacgctgt gttcaagact gatggcgagg agtttttgtg cttcaacggc aaattccagc
    48121 ggcagttctg agaacacgtc tttgcagaaa ccgttaacaa tcatcgagat ggcgtcttct
    48181 tcgctaatcc cgcgttgcag gcagtaaaac agttgatctt caccaatacg tgatgtcgtt
    48241 gcctcgtgct ccagttgtgc gctgttattg cgacattcga catacgggaa ggtatgcgcc
    48301 ccacaattag cgccaatcag cattgagtcg cactgagtga aattgcgcgc attggttgcc
    48361 gtcggcatga ttttcactaa gccgcgataa ctgttctgac tatgtccggc agagatccct
    48421 ttcgagataa tggtcgattt ggtgttttta ccgatgtgga tcatcttggt gccggtatcc
    48481 gcttgctgat gaccgctggt cagcgccact gagtaaaact caccaatgga gttatcgccg
    48541 cgcaaaatgc agctgggata tttccacgta atcgctgacc cggtttctga ttgcgtccat
    48601 gacattttgc tgttttcgcc ttcgcacaaa gcacgcttgg tgacgaaatt gagaataccg
    48661 ccggtgttgt tatcgccagg gaaccagttt tgtaccgtgg aatatttcac ctcggcgttt
    48721 ttatggatga tgacttccac caccgccgcg tgtaactgat agctgtcacg caccggagcg
    48781 gaacagcctt caatgtagct gacgtagctg tcttcgtcgg ccaccagaat ggtgcgctca
    48841 aactgcccgg ttttttccgc gttaatgcga aaataggtgg aaagttccat cgggcagcgc
    48901 acgcctttag gcacataaat aaacgtaccg tcagaggcta ccgccgcatt aagcgcggca
    48961 aagaagttgt catttcccgg caccacagtg ccgagatatt tacgcaccag ttccggatga
    49021 tcgtggatcg cctcaccaaa ggaacagaaa ataattccct gctccgccag tttttcgcga
    49081 taagtagtgg caaccgaaac agagtcgaaa atggcatcca ccgccacctc tttgccttcc
    49141 cgcacgggaa cgcccaactg ctcaaacgcc gcctccacct ctttacttaa aaaggcgttc
    49201 gcgccagttt gctgcaccgc gccaggttca gacgcgcaag tgtcgtcaca attaccgcac
    49261 gatggtgctg agtagtaact gtaatcctga taattcagct tgtcgtagtg cgctttcaac
    49321 cagtgcggtt cttccatctc cagccatgcg cgataggcgt ttagacgaaa ctccagcatc
    49381 cactccggct cattacgctt cgccgaaatt gcgcgcacca cctcttcgtt tatccccttt
    49441 gccagctcat cggtggctaa ctgggtgaag aatccttctt tataattcag cgggccgccg
    49501 gtccaggttt tgacatcgtc agttgcttca gtattacgag acatagtacc gcctataccc
    49561 caaagctttc gccacagcca cattcattct gggctttagg gttgtgaaat ttgaatatct
    49621 gattaagtcc ttcacgaacg aaatcgactt ccgtgccatc aataaacggc atcgcttgca
    49681 gcgggacaaa cagcttcgcg ccgtcgtgtt caaacagcag atcgtctttg tccggctcgc
    49741 taacactgtc gagcacatag ccaaagcccg cgcagcccgt ttgcttcacg cctaagcgca
    49801 cgccgaccat acccggctgc tttgccacca gctcacggat gtgtatcgcc gctgcgggtg
    49861 tcagcgttaa gccttgccag gcgaaatctt gtgggttaaa ggttcctgaa tgcatgtcca
    49921 tcgatttacc tcacttcatc gctttcagcg tataacagca tgctagtgat aatgattatc
    49981 agttcaaccc agcaaacgca ggggctttac cgtaaaacat gctttttgcc tgcttttaat
    50041 aagcatagac cctgatgtgt gggttaacag gcacgctaag aattaggtat ctcattgtta
    50101 gataatggtt attatctaag gagcattaag gctgtgatga aaaagaaaaa gttgtattga
    50161 aaatgactat ttaagagata ggtaaaaaag tgcagcgttc agaaataaga aaacccttaa
    50221 gtctgtgcga cacaggctta agggtttcta ccccatccgg cgcttatctc cggcactctc
    50281 agtggcttag ctcttgaagg ggcgataaga ataatctcat aaagctaacc cgccgtttta
    50341 acacaaactg cgattagtat tatttttgaa caatatcagg cggtagataa gcagtattaa
    50401 gaaggtcatc gaacctggac ggaggttaat ccaggtcgat ttggcgaact tgcggcatta
    50461 agtcaggatc aatgcacgcc cagacgccag gcaaagtaga tttcttcttt taattcagta
    50521 gaagagagag taagcaggtc agcaaattca agttctagtt gtttcagacg tttgagatat
    50581 tgggcaggtg aaagattgct ctggtcacgg cgtaaaaatt caatggccag ctgggtggga
    50641 tcaagttgag tagacatagc atcctcgctt ttagacaaga cgtgcacagt ataccaccgt
    50701 ttactgtgca gataatgacc aaaagcaata tgcgtcacac ttttctggtg acaacgtcac
    50761 aaaatggcgg tcgtcaatcg tgacgaacag cacaaacgcc ctttctcatc gaagatttca
    50821 atctgccaga cctggtgacg cgaaccgaga tgcaacggtt tacatatgcc gcgcacccgc
    50881 ccttctcgtg ccgagcggac gtgattagca ttgatttcca gaccaaccac tttttgctca
    50941 ccttcggtac ataaataacc ggcaacggaa ccgatacttt cggccagtac cacggatgct
    51001 cctccatgca gcaacccgaa aggctgcttt gtccgcgagt ctactggcat tgtcgcttca
    51061 agggtgtcat caccaatatg ttcaaagcga atatccagga accccaccat gtttccttca
    51121 cccatagcat tcagtgcttc cagggtgatt ttccgtttcc atatcattta ataatctcca
    51181 gtaaagcctg cacaggatgg cgtacccccg tgccttcaac ccgttttacc tggctacggc
    51241 aggaatatcc ggtcgccaga cagcggttac gcggcagtcg ctgcatagcc tgatgccagg
    51301 ataactcata gatcccgagc gaattttcat ggtttttcgc ttcatgtccg taagtccctg
    51361 ccatgccgca gcaacccacg ctgacatttt ccagtttcgc gccaaaacgg gcaaatatcg
    51421 cggcccattg tgctggcgcg cccggcaaag cggtaacctc ggtacagtga ccaaagaaat
    51481 accatgattc accgctgact gtagccactg gatgtgactc aagtgcgctt gccagccatt
    51541 cattcgcaag taagacgtta aactcgccac gttcctcgcc cagcgccagt ttatattcat
    51601 cgcgataaca aagtaccagc gccggatcga cgcccaccat tggcatccca agcttagcca
    51661 tacggttgag gaaatccgcc gtctttttcg ccgtcttcgc aaaacgatta agaaagcctt
    51721 tgatatgctg ggctttgcca tttggcgaaa atggcagtaa cacaggctgg aaacctaatt
    51781 tttcaaccag acggacaaaa tccgccacca cttgcgcatc gtaatagctg gtaaaggggt
    51841 cctgcaccac caacaccgtg cgcgctttct gctctgcatt gagagcttca agctgttcca
    51901 gcgtcatgtt tgccgagcga tgccccacca tttgttgttg tagcgagggg accgacagca
    51961 gtggcaaatc aaccatgccg atatgttttt ccgagagttt gcgcaccagc ggctggttaa
    52021 tgaagaagtt aaaggttttc ggcgcgcgtg ccatcagcgg tgcgtagctc tcgaccgtag
    52081 cgacgaggtg gtcgcgcagc gggcgtaaat aacgggtgtg atagagctgc aggaaacgag
    52141 agcgaaactc cggcacatca attttgatgg ggcactgggt cgaacacgct ttacaggcca
    52201 gacagcccga catcgcctct ttgacttcgt gtgagaagtc atattcgcct ttattcgcat
    52261 gccagctatt gcgcgtgcgg gcaattaacg tccgtaaact gacgccggat tcaggcagtt
    52321 cttgttccag tttgagtgga tcaacgccgc gatccgccaa caaacgcagc cattcacgca
    52381 ccagcgttgc gcgccctttc ggtgaatgaa tccggttctg ggtgatcttc atcgacggac
    52441 acatcggact acgggcatca aagttgaagc ataaaccgtt gccgttacac tccatcgcac
    52501 cgcgccactg ctggcgtacc gcaatgggga tctgccgatc gaatgtaccg cgcttcaccg
    52561 cgtccacttt catcatcggc gcatcgagac cttctggcgg gcaaatcttc cctgggttga
    52621 gtcggttatg cgggtcaaat gccgctttca ctttgcgcag ttctgcaaaa agttcctcac
    52681 cgaaaaacgc cgggctgtat tcagcgcgaa aacctttgcc gtgctcgccc cacaacaaac
    52741 caccgtattt cgcagtcagc gccaccacgt catcagagat ttgcttcatc aaaatctctt
    52801 gttgcggatc gcacatatcc agtgccggac ggacgtgcaa aacacctgcg tcgacgtgac
    52861 cgaacatacc gtagcttaag ccgtggctgt cgagcagcgc gcgaaattca gcaatataat
    52921 cggcctgatg ttccggcggt acgcaggtat cctcagcaaa cggaattggc ttagcggcac
    52981 ctttggcatt gccaagcaga ccaacggctt ttttgcgcat tgcatagata cgttcaaccc
    53041 ccgccagctc gcggcacacc tgccagccaa tcacacctgc ctgttggctg gcaatcagct
    53101 catcaagccg cacacagagt gcattaactc gctcgtcaat cagcgcctca tcatcaccag
    53161 caaattccac aatgttcagc ccgagcatct ctttgtcagg cacatcggta atcaactcgc
    53221 tgacggaatg ccagacaata tcttcccgcg ccagattcag cacttttgag tccaccgtct
    53281 ctaccgaaag cgcccgcgcc tcaaccataa acggcgcgtt acgcagcgcg gagtcaaaag
    53341 agtcatattt gacgttcacc agacggcgca ctttaggcaa gggcgtaata tccagccgcg
    53401 cttcggtaat aaaggccagc gtcccttctg aacccgtcag aatgcgcgtc aggtcgaact
    53461 cggtcatctc atcgttaaag acatgacgca gatcgtaacc ggtaagaaag cggttaagtt
    53521 tggggaagtt gtcgatgatt aactggcgtt gcagacggca acgttgataa accgtgttat
    53581 aaattcgccc gattgtggta ttggttttac ccagcgtttc cgccagttcg acgggtaaag
    53641 gttgcgtatc gagaatatcg ccccccaaca acaccgcgcg tacgccaagt acgtgatctg
    53701 acgttttgcc atagaccagc gatccctgac cggatgcatc ggtattgatc atcccgccga
    53761 gcgttgcccg gttgctggtc gaaagttctg gtgcaaaaaa gtagccgaac ggtttcaggt
    53821 actgattgag ttgatctttt atcaccccgg cctcaacgcg cacccagccc tcttcagggt
    53881 taatttcgat gatgcggttc atatggcggg acatatcaac aataatcccc tggttgagcg
    53941 cctgaccgtt agtgccggtg ccgccgccgc ggggggtaaa gatcagcgat gaatagcgtt
    54001 cctgcgcggc aagacgggcg atcagcgcca catctgcggt tgaacgcgga aataccaccg
    54061 catcggggag aagttggtaa atactgttgt cggtcgacat tgtcagacga tcggcataac
    54121 ttgtcgccgt atcgccggta aaaccttgtt gctccagctc ttgcaaaaaa ttaagcacca
    54181 gttgaacgac gccgggtgcc tgggaaatct gtggaatcat tatattgacc ctttcctgcg
    54241 gtctgtgatg taggtcgata cactattctt tcaggcggct gcaatagcgc actgaaaggt
    54301 gatgtttatt tactctatgg atttcgagtt gcaggaaggc ggcaagcgag tgaactccag
    54361 gagcttacaa tagtaagtga ctggggtgaa cgaacgtagc cgcagcgcat gcaacttgaa
    54421 atacgacgag taaatcgttt gcgtgttgcc tgagttgttg taccacattt ttttctaaca
    54481 cgcccatcag aattaagggc agaatcggcc tgttaaaaac cgctgaaatt gctcatcatt
    54541 atgcaggtga gtttcgcgtg ttcacgtcgc gtcgacgatt tgacgcacaa aaaaggtgaa
    54601 aagtagttat ggtaaatgtt cgtcagccca gggatgtcgc acaaattctg ctttcggtgc
    54661 tgtttttagc catcatgatt gtggcatgtc tgtggattgt tcaacccttt attctcggct
    54721 ttgcatgggc cggtacggtg gttatcgcca cctggccggt attgttacgt ttgcaaaaga
    54781 tcatgtttgg ccgccgctct ctcgccgttc tggtgatgac gctgttatta gtgatggtgt
    54841 ttatcatccc tattgctttg ctggttaaca gtatcgtcga cggcagcggc ccgctaatta
    54901 aagccatttc cagcggcgac atgacgttac ccgatctggc gtggcttaat accattccgg
    54961 tgattggcgc gaagctgtat gcaggctggc acaacttgct ggatatgggg ggtacggcga
    55021 tcatggcgaa agtccgccct tatattggca ccaccaccac ctggttcgtt gggcaggcgg
    55081 cgcatatcgg gcgctttatg gtgcattgtg cgctgatgct tctcttcagt gccctgctgt
    55141 actggcgcgg tgaacaggtg gcacaaggca ttcgccattt tgcaacccgt ctggcaggcg
    55201 ttcgcggaga tgccgccgtg ctgctggcgg cacaggctat ccgcgcggtg gcgctgggtg
    55261 tggtggtgac ggcgttagta caggcagtgc ttggcagtat cggcctcgcc gtatccggcg
    55321 taccttatgc aacgttgcta acggtgttaa tgatcctctc ctgccttgtc cagcttggcc
    55381 cgttgccggt actgattccg gcgattatct ggctctactg gaccggcgat accacctggg
    55441 gaacggtatt gttagtgtgg agcggtgtgg ttggcacgct ggataacgtc atccgcccaa
    55501 tgt
//
LOCUS       DAJTVJ010000012        49819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000012 DAJTVJ010000000
VERSION     DAJTVJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.012"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..455
                     /locus_tag="OO448_000693"
     CDS             <1..455
                     /locus_tag="OO448_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6335992.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OO448_000694"
     CDS             complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OO448_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6335993.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OO448_000695"
     CDS             complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OO448_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6335994.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4359..5225
                     /gene="yghU"
                     /locus_tag="OO448_000696"
     CDS             4359..5225
                     /gene="yghU"
                     /locus_tag="OO448_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6335995.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OO448_000697"
     CDS             complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OO448_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6335996.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OO448_000698"
     CDS             complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OO448_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6335997.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OO448_000699"
     CDS             complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OO448_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6335998.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6424..6918)
                     /locus_tag="OO448_000700"
     CDS             complement(6424..6918)
                     /locus_tag="OO448_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6335999.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OO448_000701"
     CDS             complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OO448_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6336000.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OO448_000702"
     CDS             complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OO448_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6336001.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OO448_000703"
     CDS             complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OO448_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6336002.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OO448_000704"
     CDS             complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OO448_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6336003.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12082..12369)
                     /locus_tag="OO448_000705"
     CDS             complement(12082..12369)
                     /locus_tag="OO448_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6336004.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12488..13375)
                     /locus_tag="OO448_000706"
     CDS             complement(12488..13375)
                     /locus_tag="OO448_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6336005.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13532..14572
                     /gene="gpr"
                     /locus_tag="OO448_000707"
     CDS             13532..14572
                     /gene="gpr"
                     /locus_tag="OO448_000707"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6336006.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14612..15106)
                     /locus_tag="OO448_000708"
     CDS             complement(14612..15106)
                     /locus_tag="OO448_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6336007.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15297..16181
                     /gene="yghA"
                     /locus_tag="OO448_000709"
     CDS             15297..16181
                     /gene="yghA"
                     /locus_tag="OO448_000709"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6336008.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OO448_000710"
     CDS             complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OO448_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6336009.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OO448_000711"
     CDS             complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OO448_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6336010.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17759..18946
                     /gene="metC"
                     /locus_tag="OO448_000712"
     CDS             17759..18946
                     /gene="metC"
                     /locus_tag="OO448_000712"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6336011.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19086..19745
                     /locus_tag="OO448_000713"
     CDS             19086..19745
                     /locus_tag="OO448_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6336012.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OO448_000714"
     CDS             complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OO448_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6336013.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20878..22041
                     /gene="yqhD"
                     /locus_tag="OO448_000715"
     CDS             20878..22041
                     /gene="yqhD"
                     /locus_tag="OO448_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6336014.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22146..22973
                     /gene="dkgA"
                     /locus_tag="OO448_000716"
     CDS             22146..22973
                     /gene="dkgA"
                     /locus_tag="OO448_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6336015.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23173..24099
                     /locus_tag="OO448_000717"
     CDS             23173..24099
                     /locus_tag="OO448_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6336016.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24150..24407
                     /gene="yqhH"
                     /locus_tag="OO448_000718"
     CDS             24150..24407
                     /gene="yqhH"
                     /locus_tag="OO448_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6336017.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24450..26669)
                     /locus_tag="OO448_000719"
     CDS             complement(24450..26669)
                     /locus_tag="OO448_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6336018.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNACIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OO448_000720"
     CDS             complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OO448_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6336019.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OO448_000721"
                     /gene_synonym="parF"
     CDS             complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OO448_000721"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6336020.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OO448_000722"
     CDS             complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OO448_000722"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6336021.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(32041..32523)
                     /locus_tag="OO448_000723"
     CDS             complement(32041..32523)
                     /locus_tag="OO448_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6336022.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OO448_000724"
     CDS             complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OO448_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6336023.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33120..33779
                     /gene="qseB"
                     /locus_tag="OO448_000725"
     CDS             33120..33779
                     /gene="qseB"
                     /locus_tag="OO448_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6336024.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33776..35125
                     /gene="qseC"
                     /locus_tag="OO448_000726"
     CDS             33776..35125
                     /gene="qseC"
                     /locus_tag="OO448_000726"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6336025.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35235..35816
                     /gene="mdaB"
                     /locus_tag="OO448_000727"
     CDS             35235..35816
                     /gene="mdaB"
                     /locus_tag="OO448_000727"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6336026.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35847..36161
                     /locus_tag="OO448_000728"
     CDS             35847..36161
                     /locus_tag="OO448_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6336027.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OO448_000729"
     CDS             complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OO448_000729"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6336028.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OO448_000730"
     CDS             complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OO448_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6336029.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OO448_000731"
     CDS             complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OO448_000731"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6336030.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39563..39985)
                     /locus_tag="OO448_000732"
     CDS             complement(39563..39985)
                     /locus_tag="OO448_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6336031.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OO448_000733"
     CDS             complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OO448_000733"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6336032.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40820..42301
                     /gene="tolC"
                     /locus_tag="OO448_000734"
     CDS             40820..42301
                     /gene="tolC"
                     /locus_tag="OO448_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6336033.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42449..43120
                     /locus_tag="OO448_000735"
     CDS             42449..43120
                     /locus_tag="OO448_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6336034.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43126..44286
                     /locus_tag="OO448_000736"
     CDS             43126..44286
                     /locus_tag="OO448_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6336035.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OO448_000737"
     CDS             complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OO448_000737"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6336036.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45255..46028
                     /gene="zupT"
                     /locus_tag="OO448_000738"
     CDS             45255..46028
                     /gene="zupT"
                     /locus_tag="OO448_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6336037.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46086..46256)
                     /locus_tag="OO448_000739"
     CDS             complement(46086..46256)
                     /locus_tag="OO448_000739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336038.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OO448_000740"
     CDS             complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OO448_000740"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6336039.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47274..47422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47544..47834
                     /gene="ubiK"
                     /locus_tag="OO448_000741"
     CDS             47544..47834
                     /gene="ubiK"
                     /locus_tag="OO448_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6336040.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48117..48668
                     /locus_tag="OO448_000742"
     CDS             48117..48668
                     /locus_tag="OO448_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR6336041.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48728..49735
                     /locus_tag="OO448_000743"
                     /pseudo
     CDS             48728..49735
                     /locus_tag="OO448_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT        12493 a        13204 c        12355 g        11767 t
ORIGIN      
        1 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
       61 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
      121 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
      181 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
      241 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
      301 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
      361 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
      421 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
      481 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
      541 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
      601 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
      661 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
      721 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
      781 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
      841 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
      901 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
      961 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
     1021 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
     1081 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
     1141 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
     1201 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
     1261 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg
     1321 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
     1381 aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
     1441 tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg
     1501 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
     1561 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
     1621 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg
     1681 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
     1741 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
     1801 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc
     1861 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
     1921 gcttcgtaga acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca
     1981 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
     2041 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
     2101 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
     2161 aacacaggta ttccactggt taatatattc acatatgaaa taaataaata ttagaacata
     2221 taaatattga atattttgat taatccccgt actgattatt cttcataatc agtacggggt
     2281 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
     2341 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag
     2401 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
     2461 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
     2521 aggtcgatat tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc
     2581 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
     2641 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact
     2701 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
     2761 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
     2821 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
     2881 ccggcagcat cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc
     2941 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
     3001 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc
     3061 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
     3121 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg
     3181 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     3241 caggagagac gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg
     3301 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
     3361 ttaattagct cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg
     3421 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
     3481 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
     3541 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     3601 atggtggtgt catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc
     3661 agctcacgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     3721 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     3781 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     3841 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     3901 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     3961 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     4021 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     4081 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     4141 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     4201 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     4261 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     4321 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     4381 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     4441 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt
     4501 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
     4561 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     4621 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     4681 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     4741 aatttggcta cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt
     4801 tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg
     4861 cgccgatgaa aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg
     4921 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     4981 cggatatggc gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg
     5041 cagagtttct tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg
     5101 aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc
     5161 agttgcatga gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg
     5221 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     5281 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag
     5341 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     5401 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     5461 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
     5521 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     5581 gaacgccaat acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg
     5641 aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct
     5701 gatgaatctc caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga
     5761 tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga
     5821 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
     5881 taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca
     5941 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
     6001 acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
     6061 tggccctctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat
     6121 tgcgaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
     6181 ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact
     6241 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa
     6301 tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
     6361 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
     6421 tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc
     6481 cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg
     6541 tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt
     6601 gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt
     6661 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt
     6721 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
     6781 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
     6841 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
     6901 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc
     6961 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
     7021 agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg
     7081 tcatcattga agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta
     7141 ctgataatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
     7201 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga
     7261 tcgcctttgc cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat
     7321 tcgcagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact
     7381 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
     7441 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
     7501 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
     7561 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
     7621 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
     7681 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
     7741 tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
     7801 gtcagccatt ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca
     7861 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
     7921 acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg
     7981 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
     8041 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
     8101 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
     8161 ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc
     8221 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
     8281 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
     8341 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
     8401 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
     8461 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
     8521 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
     8581 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
     8641 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
     8701 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
     8761 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
     8821 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
     8881 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
     8941 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
     9001 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
     9061 gactcttttc atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga
     9121 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
     9181 agctctgcca caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag
     9241 actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca
     9301 gacgcttgag agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg
     9361 ccatcacgcc gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
     9421 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
     9481 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
     9541 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
     9601 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
     9661 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga
     9721 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
     9781 gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
     9841 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
     9901 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
     9961 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    10021 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    10081 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    10141 atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    10201 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    10261 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    10321 cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    10381 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    10441 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    10501 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    10561 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    10621 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    10681 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    10741 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    10801 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    10861 caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt
    10921 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    10981 ctttatggaa gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg
    11041 gccacacgcc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    11101 ctttacagcc gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt
    11161 tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    11221 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    11281 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    11341 cggttttgcc tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg
    11401 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg
    11461 ccgctttgcg gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat
    11521 cttttaatgg gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg
    11581 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    11641 ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    11701 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    11761 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    11821 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    11881 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    11941 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    12001 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    12061 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    12121 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    12181 gcggcgcgtc agaatacccg cttcccaggc actaagctgg cgatctccgg tcttttcgta
    12241 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    12301 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    12361 gttattcatc gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa
    12421 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat
    12481 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    12541 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    12601 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc
    12661 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    12721 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    12781 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    12841 tgcctgccgc ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    12901 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac
    12961 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    13021 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    13081 gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    13141 ttggcgaagg atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    13201 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    13261 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat
    13321 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    13381 cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc
    13441 gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg
    13501 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    13561 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    13621 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg
    13681 taaagcgttt gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    13741 aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga
    13801 tgaactgatt atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg
    13861 cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga
    13921 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    13981 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    14041 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt
    14101 aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga
    14161 taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct
    14221 gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa
    14281 agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt
    14341 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    14401 gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga
    14461 gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca
    14521 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa
    14581 taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    14641 gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca
    14701 gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    14761 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc
    14821 agttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg
    14881 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    14941 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    15001 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    15061 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    15121 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac
    15181 atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat
    15241 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    15301 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    15361 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    15421 ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag
    15481 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    15541 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    15601 ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag
    15661 cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    15721 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    15781 tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca
    15841 tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta
    15901 cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta
    15961 ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac
    16021 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    16081 cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    16141 cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    16201 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    16261 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    16321 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    16381 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    16441 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    16501 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    16561 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    16621 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    16681 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    16741 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    16801 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    16861 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    16921 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    16981 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    17041 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    17101 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    17161 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    17221 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    17281 gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc
    17341 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg
    17401 actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga
    17461 tacatacccc agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag
    17521 gacgcaagtc acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt
    17581 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    17641 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    17701 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat
    17761 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    17821 cggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc
    17881 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    17941 gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt
    18001 gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg
    18061 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    18121 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    18181 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat
    18241 ggaagtccac gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat
    18301 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat
    18361 cgatgtttct attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    18421 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg
    18481 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    18541 gcgtttgcgt caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc
    18601 gcaagtagcg cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa
    18661 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa
    18721 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    18781 cgggtatgaa tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca
    18841 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    18901 cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg
    18961 gacaattttg cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    19021 gtcccggacg aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    19081 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    19141 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    19201 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    19261 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    19321 caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg
    19381 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    19441 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    19501 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    19561 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    19621 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    19681 tgttaaccgc tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg
    19741 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    19801 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    19861 ctaaattgcg atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg
    19921 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    19981 gaggtgctgg tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct
    20041 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg
    20101 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    20161 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    20221 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    20281 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    20341 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc
    20401 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    20461 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    20521 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    20581 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    20641 ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta
    20701 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    20761 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    20821 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    20881 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    20941 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg
    21001 aaaaaaatcg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    21061 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    21121 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    21181 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    21241 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    21301 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    21361 gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    21421 accctgccgc cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa
    21481 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    21541 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    21601 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg
    21661 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    21721 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    21781 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    21841 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    21901 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    21961 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    22021 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    22081 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    22141 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    22201 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    22261 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    22321 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    22381 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    22441 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    22501 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    22561 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    22621 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    22681 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    22741 agaaagtcat tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc
    22801 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    22861 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa
    22921 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa
    22981 attctcccgg tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    23041 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    23101 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    23161 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    23221 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    23281 aaatcagatg ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    23341 cgtattcaac aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg
    23401 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt
    23461 gcgataaccc aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    23521 gatgataaag aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    23581 attgacgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    23641 aaaacgctgg ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa
    23701 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    23761 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    23821 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    23881 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    23941 catgttcgtt ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg
    24001 cgcgaaggca agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    24061 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    24121 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    24181 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    24241 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac
    24301 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc
    24361 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    24421 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    24481 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    24541 gtctgacgct gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    24601 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    24661 ctgccaatca gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt
    24721 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    24781 acgaagacgt cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    24841 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    24901 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    24961 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    25021 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    25081 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    25141 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg
    25201 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    25261 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    25321 tcagcgcgtg gcgatttgca gtgcaacata tacatgttgg cagttggccc accgagatcg
    25381 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    25441 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    25501 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    25561 atacaggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    25621 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    25681 tgtttttgca tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa
    25741 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag
    25801 gttttttccc acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt
    25861 ttcggtgcca ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt
    25921 gggtcgattt ttccaggggt atcaagacgg ggggaatcca cgccgctcca gccaggcagc
    25981 gcctctttca cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    26041 atcgccagac ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg
    26101 tcggctttcg aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt
    26161 gcggtacggc gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc
    26221 tctttacaac gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta
    26281 tccggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc
    26341 gaatccatgt tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag
    26401 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc
    26461 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    26521 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    26581 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    26641 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    26701 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct
    26761 cctcgggcga gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac
    26821 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    26881 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    26941 tgtcttccgg gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa
    27001 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    27061 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    27121 gactgatatc gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca
    27181 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg
    27241 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    27301 cttcgccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    27361 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    27421 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    27481 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    27541 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    27601 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat
    27661 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    27721 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    27781 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    27841 gtgccggacc gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat
    27901 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    27961 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    28021 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    28081 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    28141 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    28201 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    28261 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    28321 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    28381 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    28441 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    28501 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    28561 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    28621 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    28681 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    28741 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    28801 gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    28861 cagcggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    28921 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    28981 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    29041 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    29101 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    29161 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca
    29221 ggccctcccc ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga
    29281 tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac
    29341 cagtgacttt ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg
    29401 tcagcgtgct ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg
    29461 cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat
    29521 cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag
    29581 catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct
    29641 tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat
    29701 cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc
    29761 caggcggcaa cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg
    29821 taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc
    29881 tcttaccttt caccgccact ttgaagctat cacccgcttt ataattcagg cccggcgcgt
    29941 cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag
    30001 gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg
    30061 gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt
    30121 tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct
    30181 cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt
    30241 cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg
    30301 gttcatcttc gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca
    30361 aaccttccag gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc
    30421 gcacggtatc gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg
    30481 ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg
    30541 tggtagcgaa gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa
    30601 tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca
    30661 gctttttgtt gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat
    30721 gcggtaatgc gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgta
    30781 ctgaaccacg tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg
    30841 cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg
    30901 gctggtcgat taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat
    30961 cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag
    31021 caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc
    31081 cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac
    31141 gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca
    31201 gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat
    31261 cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt
    31321 tggcgctggc attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt
    31381 tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta
    31441 agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt
    31501 ctgattcctc aactgattcg ccagccttca aaggggcaat attgccggag atactacatt
    31561 atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg
    31621 gcgataaagg catcaatgtg ccttgatata caacgctttg ctgtagactt gatcggcata
    31681 gagggcagca caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg
    31741 attcgagaag ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgacta
    31801 taagtatatg gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca
    31861 taagtgcggc ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt
    31921 tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg
    31981 gcattgttcg ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca
    32041 tcacgctaac ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag
    32101 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    32161 gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc
    32221 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    32281 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    32341 gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    32401 acgccacatg ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg
    32461 caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    32521 cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg
    32581 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    32641 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt
    32701 cgatatcaac attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc
    32761 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    32821 ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg
    32881 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta
    32941 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    33001 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta
    33061 cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa
    33121 tgcgaatttt actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta
    33181 gtaaaatggg ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    33241 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    33301 atattttgcg cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc
    33361 gtgatgcgtt agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    33421 aaccttttgc gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg
    33481 gccaggccag caacgagctg cgccacggta acgttatgct cgaccccggc aaacgtatcg
    33541 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    33601 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    33661 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    33721 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    33781 atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc
    33841 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    33901 attgttcgac acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga
    33961 aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga
    34021 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg
    34081 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    34141 ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    34201 catcgttgtt ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    34261 gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact
    34321 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    34381 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc
    34441 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc
    34501 cgatgcagct cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc
    34561 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    34621 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    34681 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat
    34741 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca
    34801 cagcatcaaa cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga
    34861 taacgcagtg cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa
    34921 tttcatcgtg agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga
    34981 acgcttctat cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt
    35041 ccagcgaatc gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg
    35101 atttgaggcg aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg
    35161 cacattttgc taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac
    35221 cttgaggtta aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact
    35281 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
    35341 ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa
    35401 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
    35461 ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca
    35521 gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg
    35581 gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag
    35641 atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc
    35701 aattccttgg catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
    35761 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
    35821 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
    35881 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
    35941 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
    36001 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc
    36061 ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg
    36121 tgctggagat gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg
    36181 ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct
    36241 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
    36301 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
    36361 gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct
    36421 ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt
    36481 ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga
    36541 cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca
    36601 gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac
    36661 gaagctggct cagatcgtcg ctgttaggat cgataccgat cgctaccgaa atatcatgca
    36721 cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt
    36781 tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg
    36841 agtcaccttc cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca
    36901 gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac
    36961 gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt
    37021 tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt
    37081 gacgcgaaga gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg
    37141 acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca
    37201 gaatgttgcg atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat
    37261 taacatgggt accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt
    37321 caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat
    37381 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
    37441 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg
    37501 taatctcaac gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa
    37561 cagaaaatcg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
    37621 tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa
    37681 aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg
    37741 cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
    37801 agaatttacc gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt
    37861 ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag
    37921 catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat
    37981 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
    38041 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
    38101 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
    38161 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
    38221 tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
    38281 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
    38341 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga
    38401 ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc
    38461 agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
    38521 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa
    38581 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
    38641 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
    38701 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc
    38761 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
    38821 cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg
    38881 ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca
    38941 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
    39001 gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca
    39061 acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
    39121 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
    39181 caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg
    39241 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
    39301 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
    39361 caatcaggtc gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt
    39421 cccaggtgtt tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa
    39481 tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
    39541 ggtgtcctta ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac
    39601 agtatcgcaa ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca
    39661 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
    39721 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
    39781 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
    39841 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
    39901 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
    39961 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
    40021 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
    40081 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
    40141 gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
    40201 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
    40261 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta
    40321 tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc
    40381 gaatctgctc aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg
    40441 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
    40501 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
    40561 taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta
    40621 atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
    40681 gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
    40741 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
    40801 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc
    40861 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
    40921 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
    40981 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
    41041 cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac
    41101 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg
    41161 gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc
    41221 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc
    41281 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
    41341 cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg
    41401 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
    41461 actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct
    41521 gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct
    41581 ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc
    41641 ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac
    41701 ccagtatgac gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat
    41761 ttatcagggc ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc
    41821 cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa
    41881 caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca
    41941 aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt
    42001 gttggatgca accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa
    42061 ctacctgatt aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct
    42121 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc
    42181 gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt
    42241 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
    42301 acgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
    42361 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
    42421 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
    42481 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
    42541 ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga
    42601 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
    42661 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
    42721 atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca
    42781 ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
    42841 ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
    42901 cccagccagc ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc
    42961 ccagccaggc cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac
    43021 tactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
    43081 tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
    43141 attaccgagc gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc
    43201 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt
    43261 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
    43321 gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt
    43381 gtgcgtcagt catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg
    43441 gatggcactg gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac
    43501 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
    43561 gagggcagcg accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg
    43621 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
    43681 cgcggaacca ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc
    43741 ctctacattg acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag
    43801 gtaatttcca acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc
    43861 accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
    43921 aacaaagcgc ttctaccgct actatgggag atgttcccga atcacccgaa cctgctgccc
    43981 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
    44041 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
    44101 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
    44161 gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt
    44221 cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt
    44281 gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg
    44341 acagcatact caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc
    44401 catcccacgc acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata
    44461 acgcgccacc ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc
    44521 acgtcagatt cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat
    44581 caccgtgcca cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta
    44641 ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt
    44701 tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca
    44761 gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg
    44821 acgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg
    44881 tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg
    44941 cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc
    45001 gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt
    45061 tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat
    45121 ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac
    45181 ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt
    45241 ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt
    45301 ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt
    45361 cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg
    45421 cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc
    45481 ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa
    45541 aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca
    45601 tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg
    45661 agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag
    45721 tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga
    45781 tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga
    45841 tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct
    45901 cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg
    45961 gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa
    46021 ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga
    46081 aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc
    46141 gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg
    46201 aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt
    46261 ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc
    46321 ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa
    46381 aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc
    46441 atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt
    46501 aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt
    46561 cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac
    46621 gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg
    46681 ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg
    46741 cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat
    46801 cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac
    46861 cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta
    46921 ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac
    46981 cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga
    47041 tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac
    47101 gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg
    47161 tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta
    47221 ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact
    47281 ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga
    47341 gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt
    47401 tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc
    47461 atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca
    47521 cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc
    47581 aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa
    47641 tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg
    47701 acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa
    47761 tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc
    47821 ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt
    47881 tacagtgctt tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa
    47941 tgagtgcaat tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta
    48001 gataaactaa tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc
    48061 ttaaataaca gttactaatc attcattttc tacttatatt tatataaagg aatattatgt
    48121 ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat
    48181 ttgctgacga aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt
    48241 caattaaacc aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc
    48301 tgaaaagcga ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt
    48361 tgagccaggg tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta
    48421 tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag
    48481 gcgttggcgt acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag
    48541 taattacatt caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat
    48601 ccccgggcaa agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt
    48661 acgagtaatc acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt
    48721 ggaacagatg tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta
    48781 ttgttcatta tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa
    48841 agatttaatc gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg
    48901 ccaattaccc gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat
    48961 ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga
    49021 gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat
    49081 cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa
    49141 aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac
    49201 gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc
    49261 ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc
    49321 tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa
    49381 caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat
    49441 cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga
    49501 ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct
    49561 acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc
    49621 acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa
    49681 tttattcaat acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggccagt
    49741 ctatatatca atgacactaa ccattacatg aattatcaga atgaaaaaga gagttatatt
    49801 gtcacgttta accagtatc
//
LOCUS       DAJTVJ010000013        49616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000013 DAJTVJ010000000
VERSION     DAJTVJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.013"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..1274
                     /locus_tag="OO448_000744"
                     /pseudo
     CDS             54..1274
                     /locus_tag="OO448_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1247..1876
                     /locus_tag="OO448_000745"
     CDS             1247..1876
                     /locus_tag="OO448_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6336042.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO448_000746"
     CDS             complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO448_000746"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6336043.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3146..4234
                     /gene="hcxA"
                     /locus_tag="OO448_000747"
     CDS             3146..4234
                     /gene="hcxA"
                     /locus_tag="OO448_000747"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6336044.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4244..4441)
                     /locus_tag="OO448_000748"
     CDS             complement(4244..4441)
                     /locus_tag="OO448_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6336045.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OO448_000749"
     CDS             complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OO448_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6336046.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OO448_000750"
     CDS             complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OO448_000750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6336047.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OO448_000751"
     CDS             complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OO448_000751"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6336048.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OO448_000752"
     CDS             complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OO448_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6336049.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OO448_000753"
     CDS             complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OO448_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6336050.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OO448_000754"
     CDS             complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OO448_000754"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR6336051.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12112..13077
                     /gene="fepB"
                     /locus_tag="OO448_000755"
     CDS             12112..13077
                     /gene="fepB"
                     /locus_tag="OO448_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6336052.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OO448_000756"
     CDS             complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OO448_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6336053.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14519..15523
                     /gene="fepD"
                     /locus_tag="OO448_000757"
     CDS             14519..15523
                     /gene="fepD"
                     /locus_tag="OO448_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6336054.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15520..16512
                     /gene="fepG"
                     /locus_tag="OO448_000758"
     CDS             15520..16512
                     /gene="fepG"
                     /locus_tag="OO448_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6336055.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16509..17324
                     /gene="fepC"
                     /locus_tag="OO448_000759"
     CDS             16509..17324
                     /gene="fepC"
                     /locus_tag="OO448_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6336056.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OO448_000760"
     CDS             complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OO448_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6336057.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OO448_000761"
     CDS             complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OO448_000761"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6336058.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OO448_000762"
     CDS             complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OO448_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6336059.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OO448_000763"
     CDS             complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OO448_000763"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6336060.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24214..26454
                     /gene="fepA"
                     /locus_tag="OO448_000764"
     CDS             24214..26454
                     /gene="fepA"
                     /locus_tag="OO448_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6336061.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26629..27249
                     /gene="entD"
                     /locus_tag="OO448_000765"
     CDS             26629..27249
                     /gene="entD"
                     /locus_tag="OO448_000765"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6336062.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OO448_000766"
     CDS             complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OO448_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6336063.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="OO448_000767"
     CDS             complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="OO448_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6336064.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28466..29584
                     /locus_tag="OO448_000768"
     CDS             28466..29584
                     /locus_tag="OO448_000768"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6336065.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            29650..29898
                     /locus_tag="OO448_000769"
                     /pseudo
     CDS             29650..29898
                     /locus_tag="OO448_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            29963..30331
                     /locus_tag="OO448_000770"
     CDS             29963..30331
                     /locus_tag="OO448_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6336066.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30425..31078
                     /gene="nfsB"
                     /locus_tag="OO448_000771"
     CDS             30425..31078
                     /gene="nfsB"
                     /locus_tag="OO448_000771"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6336067.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31186..32433
                     /gene="ybdG"
                     /locus_tag="OO448_000772"
     CDS             31186..32433
                     /gene="ybdG"
                     /locus_tag="OO448_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6336068.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="OO448_000773"
     CDS             complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="OO448_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6336069.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="OO448_000774"
     CDS             complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="OO448_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6336070.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="OO448_000775"
     CDS             complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="OO448_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR6336071.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="OO448_000776"
     CDS             complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="OO448_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6336072.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="OO448_000777"
     CDS             complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="OO448_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6336073.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40268..40951
                     /gene="cusR"
                     /locus_tag="OO448_000778"
     CDS             40268..40951
                     /gene="cusR"
                     /locus_tag="OO448_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6336074.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40941..42389
                     /gene="cusS"
                     /locus_tag="OO448_000779"
     CDS             40941..42389
                     /gene="cusS"
                     /locus_tag="OO448_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6336075.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            43126..45027
                     /locus_tag="OO448_000780"
     CDS             43126..45027
                     /locus_tag="OO448_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6336076.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            45055..45516
                     /locus_tag="OO448_000781"
     CDS             45055..45516
                     /locus_tag="OO448_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR6336077.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            45536..49616
                     /locus_tag="OO448_000782"
                     /pseudo
     CDS             45536..49616
                     /locus_tag="OO448_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
BASE COUNT        11927 a        13655 c        12938 g        11096 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
     1261 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
     1321 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1381 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1441 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1501 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1561 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1621 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1681 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1741 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1801 gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc
     1861 ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     1921 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     1981 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
     2041 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2101 tctccagccg gctttcattt aaggcattta ccagaatatc gcgcttctgg cgataaaagt
     2161 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2221 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2281 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
     2341 aagaaaccgc cactgcccgc tcacgcagct gcgaatgcgc cagcacactg gcatggccct
     2401 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2461 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggagttat
     2521 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2581 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
     2641 cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
     2701 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
     2761 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
     2821 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
     2881 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
     2941 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
     3001 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
     3061 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
     3121 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
     3181 ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga
     3241 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
     3301 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
     3361 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg
     3421 cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
     3481 atttgttgcc gttccgacga tcgccgctac ctgcgccgcc tggacaccgc tctccgtctg
     3541 gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
     3601 gctggtggaa ccagagatta tcctcaacgc gccgcaacaa tatctgctgg cggggatcgg
     3661 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
     3721 gctaaccgtg cgactgggga tcaataatgc gcaggccatt cgcgacgtat tgttaaacag
     3781 tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt
     3841 ggtggatgct attattgctg gtggtgggat ggtcggtgat ctgggcgatc gttttacgcg
     3901 tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt
     3961 tctccacggc accaaagtcg cttatggcat tctggtacaa agcgccttgc taggtcagga
     4021 tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga ccacgctggc
     4081 ggagctggaa atggatatca ataatcatgc ggagatcgac aaagtgatta cccacaccct
     4141 gcgtccggtg gaatacattc attacctgcc agtcacgctg acaccagata cgttgcgtgc
     4201 agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc
     4261 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg
     4321 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
     4381 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
     4441 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc
     4501 cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccatcgg ggagagggtt
     4561 agggtgagga ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga
     4621 ctttagtgtg cgccttttgc ctgcgccacg atctcctcga cattttccgg cattggctca
     4681 tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgccagtgc cgacttaatc
     4741 gagaacagcg ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga
     4801 cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg
     4861 ctgtctatca ttgcctggaa cttattagca atggccagga agccgacttt cgcatccggg
     4921 ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gcagccaggc cgttggtacc
     4981 agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc
     5041 agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaatg
     5101 ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc
     5161 gctgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg
     5221 cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga
     5281 ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca
     5341 cccagcgcac cgtgcaggat gtaagccatc cccaccgcca gcgtcggcgc accgcccgca
     5401 cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt atccggagta
     5461 atcgcaaagc cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct
     5521 gccggagcca gcaccgccat cgggctgttc atggcgaagt acacgcccgg atcgatgata
     5581 caggcggaaa ccagtgccat aatcgccacg aaggattcca ttaacattcc accgtagccg
     5641 ataaagcacg cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc
     5701 gcatggaagc ctgacaccgc gccacaggcg atggtgataa acaggaacgg gaacagatta
     5761 ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc
     5821 ggacgcataa tcaaaatgcc taccgccaga ccaacgatag tcccgatttt caggaaggta
     5881 gagaggtagt cacgcggggc cagcagtaac cacaccggca gcaccgccgc cacaaaaccg
     5941 tagcccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc
     6001 gggctttctg ccacccagcc gccagagata atggcaaaaa taaggaacac caggccaatc
     6061 actgacactt cgccaatacg ccccggacgc agatagcgca ggtagattcc cataaaaatc
     6121 gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc
     6181 accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca ggctaccagc
     6241 gcaatcaccc cggcagttgg ccccatctct tctttgacca gctcacccag cgagcgaccg
     6301 tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc
     6361 ccggcgagca gccagatcat ccccggcagg tagcccattt gcgctgccag caccggcccc
     6421 accagcggcc ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc
     6481 gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca
     6541 accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata
     6601 cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg
     6661 tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat
     6721 ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg tttctataac
     6781 atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa
     6841 cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc
     6901 tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt
     6961 tcatcgaaaa cgatgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag
     7021 acgccgcgta ccttgccttc agacatcggg cgatgatggg tggcgttaag ttccgtaccc
     7081 accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc
     7141 agcgccgccg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc
     7201 ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc
     7261 accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc
     7321 atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc
     7381 tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt
     7441 tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg
     7501 cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca
     7561 cattacagcg cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca
     7621 gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg
     7681 acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa
     7741 acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttactga
     7801 gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg tccagtcgct
     7861 ccgtttcagc taacagtcgc tgacatacct gcgcgacctg cgcagcatcg gcaacatcca
     7921 tcacttcggt cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa ccggtaactt
     7981 tcgctcccgc ttcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg
     8041 ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca
     8101 ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcgc catgcacttt
     8161 gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt
     8221 gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc
     8281 cgctttcctg gcggggacag gtgctggcag taatccttca gtcatcacca cccggccaga
     8341 ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc
     8401 atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca
     8461 gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat
     8521 ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc
     8581 ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg
     8641 cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt
     8701 gtaataaacc gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat
     8761 cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc
     8821 atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat
     8881 atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc
     8941 ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga
     9001 ccgccgtcag cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa
     9061 taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca
     9121 gataagcaca gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga
     9181 tcaccgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc
     9241 cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag
     9301 agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg
     9361 gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc
     9421 cttgcggcag tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg
     9481 ggtaaccctg ggtatggata attttctccg cgctatcatc aagtcgggtg tagttcacca
     9541 gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg gaaatacgcg
     9601 ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct
     9661 gcgcccggct ttcgccttca gccagcgcct gcaaccacag gctaactgcc ggcggcacca
     9721 gcgcggtaac gttaacctga tgttttgcaa tcaacgggaa gcaaagcgtg gcgctgggat
     9781 cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct ggcgaactca
     9841 tagcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac
     9901 aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagct
     9961 tcggtgtacc ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg
    10021 gcgtagcagt aaaaccgtct gccggatggt taatcgcatc ctgcaagtta tgctcaccgc
    10081 tgtcgttgag cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga
    10141 aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagtaacgcc ggttcaatct
    10201 ggctggcata ggcgttcagt tcactacgct gatggctgaa caacgccagc accggcgcaa
    10261 cgcccagttt cagcagtgcg aaaaaggtaa tgtaaagttc agcgacgtta cccagttgta
    10321 ccagcgcggt ttcaccgggt ttaatgccct gacggcgtaa actacacgcg aggttatccg
    10381 ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt
    10441 cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt
    10501 cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc
    10561 cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact
    10621 cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt
    10681 cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc
    10741 aacccacaat gccgccaaac agttcgcggt caaacggctc cagttcagca ataacctggg
    10801 tcgcggcctg atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga caggccagag
    10861 tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg
    10921 tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca
    10981 gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca
    11041 gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg
    11101 ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc
    11161 ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg
    11221 caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca
    11281 atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa
    11341 ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact
    11401 ggctgcgggt aaaacagtgg gctgaggtct gtttttcctg acgggagaac gactgccagg
    11461 attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca
    11521 ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga
    11581 agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg
    11641 acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca
    11701 tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa
    11761 ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact
    11821 atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa
    11881 tgaaaatgag aagcattatt gatgaattcg cataagcgca atgtgatgtc ctgcgccgtt
    11941 ttgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct
    12001 ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt
    12061 gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc
    12121 gccccgctct accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta
    12181 gttcaggccg ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa
    12241 agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt
    12301 gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag
    12361 ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc
    12421 ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca
    12481 accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta
    12541 atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc
    12601 gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg
    12661 aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca
    12721 cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc
    12781 tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat
    12841 gacatcattc agcttggtgg ggaaaatctg gctgctgggt taaacggtga atcgctattc
    12901 ctgttcgccg gtgatcagaa agacgccgat gcgatttatg ccaatccact gctcgcgcac
    12961 ctgcctgcag tacaaaacaa gcaggtttat acgctgggaa ccgagacgtt ccgcctggat
    13021 tactacagcg ccatgcaagt gctggacaga cttaattcac tgttaaaaaa agattgataa
    13081 aacatacaat tagagcacta ataatcccct cgcccctttg gggagagggt tagggtgagg
    13141 ggaaaatgca cctttagcta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    13201 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    13261 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccaa cagcgccgcg
    13321 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    13381 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    13441 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    13501 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    13561 tgtgccagct tcccgctggt taacgcacca atagccgcac cgagcgggat cgccgcatag
    13621 agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc
    13681 cgcaccgcgc tcgccatcgt caataaacca cccagcaacg caatccctcc taccagcggg
    13741 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc
    13801 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    13861 gccgccagcc cgtagttcca ggctacgccg ccggtcgcca gcaataaacc gccaatcatg
    13921 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    13981 ttttcacgcc ctaccagtgc aggtgttgcc gccagtagcg ccgtaacacc aagtgatgcg
    14041 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc
    14101 gcgttcaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    14161 tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    14221 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    14281 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    14341 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    14401 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    14461 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    14521 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    14581 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgcgt cagtagtgct
    14641 ggaggccttc tcaggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    14701 gccgcgcacc cttgccggtt tacttgcagg cggtgcgctt gggcttgctg gggcgttaat
    14761 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga acgccggagc
    14821 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    14881 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta tcggcagtca
    14941 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    15001 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    15061 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    15121 cccggttctg atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag
    15181 cctcggcagt gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg
    15241 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    15301 tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc
    15361 gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg
    15421 ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    15481 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga
    15541 ttactcatca cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta
    15601 cgcagcggtg ccgtcacgct ggaaatctcg caggtattcg ccgcgttgat gggcgatgcg
    15661 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    15721 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    15781 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg
    15841 atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt
    15901 gtcacttctc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    15961 attattatcg gcatcggcgt tcgcgccatg ctggtggcct ttaatacttg gctgttgctg
    16021 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc
    16081 ctgacgtggg caaaaacttc gccttccgca cccatcatca tattgatgct cattgccgcc
    16141 gccttgctgg tgcggcgaat gcgcttgctg gagatgggcg atgataccgc ctgcgcgctg
    16201 ggcgtcagcg tcgagcgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    16261 gcggcaacgg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    16321 cgacgcatta gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggagcgctg
    16381 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    16441 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    16501 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg
    16561 gcaaatatac cgttgcggaa aatctgactg tagaaattcc tgatggtcac ttcacggcaa
    16621 ttatcggacc aaatggctgt ggtaaatcca cgttactgcg taccttaagc cgcctgatga
    16681 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    16741 aagttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg
    16801 tgcaggagct ggtggcgcgt ggacgttatc cgcatcaacc gctgtttacc cgctggcgca
    16861 aagaggatga agaagcggta acgaaagcga tgcaggccac gagaataact catctggcag
    16921 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc
    16981 tagcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    17041 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    17101 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    17161 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    17221 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    17281 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    17341 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca
    17401 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    17461 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgacat
    17521 ttgcttttgt taattgctcg acaagatact gccgattacg taattcaccg ttcagttccg
    17581 caacgtcagt aaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    17641 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    17701 ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    17761 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    17821 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    17881 tatacccgct caaaacggtc tgcgcctctt tactggttgg cgcggtaaaa cttagcgtcc
    17941 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    18001 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    18061 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaacagact
    18121 gaaacttctt gataaacagg ttaaatgcac cgccacggtc gatattaaca tcgagatcaa
    18181 gaacgcggag tttggtaaag gttttctcca ggtcctgcca ctgaatggct tcggcgggag
    18241 taataacggc tgagctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc
    18301 aggcaaatgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcgatta
    18361 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca gaaaaatgag
    18421 cgtcacttcc ctgtttaata ttcaatgatg acatggggta taacctgaaa atgagtccaa
    18481 taggggaaaa tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag
    18541 tttgtaaatt ccttccactg aatgcaatat tcactctatt taagagaaat attcatcaaa
    18601 cctataaatc aaacaaataa aaaacccata attattttct gtaattatgg gtttatagat
    18661 aatattaatt tacctgttta gcgttgcgcg aataatcggc ccaattttta caaacgcccc
    18721 tggagagata atatccacat gcgcacaatc ctgacgatag atatccagct ccgcaatcca
    18781 cggcgaccag gcgcgttcag gactcatacc ttcctgaagc gtgcgttcag caacaaacaa
    18841 cgtcgcttta ccgtcaaacg gtacgctatg agcagtcgtc agcaggcgca cagcatcagc
    18901 gtagttgcct tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag
    18961 gaaggcctcg cgctcgcggt taatctccgc cagcacttcc gggtccagac cattagcttc
    19021 tttttcctgc cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac
    19081 ctgttcgcca cgggcacgca gtcgcgccgc aataccctgc gccagcgtac cgccaaggga
    19141 ataccccagc aggtaataag ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc
    19201 ttcgcagact tcatccaggt ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg
    19261 aatgccaata atcgaccatt gtggatcgag ataacgcgag agtacgctaa actgccaggc
    19321 aaaaccggac gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag
    19381 aatggtttcg aatcccatgc gccgggagct gtcctcttca ccatcaataa tcgttgccag
    19441 tttggcgacg gttgacgcga ccatcacctg ccccggcgtc acctgacgag caaactgtcg
    19501 acttaactgc gctgccagtt tcattgccag tagcgaatga ccgcccagcg caaagaaatc
    19561 agcatcggca tcctgcacgt cacaacccag caacgacgcg aatgccgcgg cgataatcgt
    19621 ttcactgccc gctttcggtg cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa
    19681 ggctttgcga tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac
    19741 caccggtacc atatgtggtg gcaatgtttc acgaagctgc gcctgcaatg cgctggtatc
    19801 caacggcagg cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt
    19861 ggctgccgcc tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc
    19921 ctgcatcacg cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc
    19981 gtcactgcgc ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt
    20041 acggtacatc cgttcgccag gagcaaaagg atcggcaata aagcggctgg cggtcagatc
    20101 ggggcgtccg agatagccct gcgccagttg aatgccggta agatagagat cacccgccac
    20161 acccggcggc accggatgca tcatcgcatc aagaatacgc agccccatgt tccacaccgg
    20221 ataaccaatc ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata
    20281 ccagctgaca tctaccgccg cttccgtcgg gccatacaga ttatgtaacg gcgcaccggt
    20341 taactgttgc cattcacggc ataaatcagc cggtaaggct tcaccactac agaaaacctg
    20401 tttcaacgtc gcgcaactct ggcgagcggt ttgcggagtc agcgaggcaa caaatgccgc
    20461 cagcatcgac ggcacaaagt gcgtggtcgt tacgccatat tcggcaaaga attgctgcat
    20521 agcgagcggg tcacggtgcg cttccggttc agccatcacc agttttgccc ctgcgataaa
    20581 cggccagaaa aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac
    20641 atcttcgcct gtaagcgggt agtgattttg catccataac aggcggttaa cgatagccgt
    20701 ctgcccgacc attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc
    20761 cgtgtggtgc ggttgtgaaa gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt
    20821 atagcaaagg cttgttaaat tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt
    20881 aattaacagc gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc
    20941 ggtatccagc ggtagccagg ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa
    21001 aaagaccgag cgcggtagtg ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg
    21061 cagcagattc gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg
    21121 cgcatctgcc agcgccggag catccggtgt ttttgccgct tgttctgcca ccagcgcgct
    21181 cagcgtggtt tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatactc
    21241 acctggcagc ataatatcga catcgccgca caacagcgcc ggatccgcgg cgaactgggc
    21301 aatcagcatt ttcaggcgtt cagcatgctg gattaacgtt ggctcatcgt aacgctgttt
    21361 attggcgagg atctcaatac tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag
    21421 gtcattaacc ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag
    21481 ttggtaatca aataccttaa tattgagtac cggaccaaac agcggttcat cacctgccgc
    21541 tcgcccgctg tcacggacaa tttgttcggc atcgtaacgt tgatggcgac gcattttttt
    21601 cagttgagcc gccagtcggg ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat
    21661 acccaacggt aaaacgttga gcacgggtcc gctagccgtc agcgccgccg agcccagtcg
    21721 acgcataaag ataaatccgg cggcatagtc catgcgattg cacaatcgcc ccagccacaa
    21781 ggctgccagc gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg
    21841 gcggaattcc ccgtcggtaa attccagttt caggcgcaga atatctgccg aagcgctgcg
    21901 ccccggtaaa ggtgccggag aaagtgacgc gggcggcggc agttgacgac gctgttctgc
    21961 ccagaatgcc gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac
    22021 tacatcagcg aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgtgca
    22081 gtaaatattg gcgatctggc gagtaatggc cgggaaactg aagccatcga ccagcaaatg
    22141 gtgataacgc tgataccagt accagcggtt atccgcaaca tgtatcagct gatgaaagac
    22201 cagtggttta ccgctatcga cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg
    22261 cgcagtaccg tgcggatcaa tattggttcg caggtcgata atttctggct gttcgaatat
    22321 cagcgcatca tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcaa
    22381 cgtatctgct tgcgctagtc cggcaaccac cgcgcgggcc agtaatggcg catcaacatc
    22441 tccagttaac tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc
    22501 tgccatccag atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc
    22561 tgcaactggg tgaaattggc cggtgtcaga gtacgccagt ggacttccag ccactgctgg
    22621 caggacgcct gtgactgcgg ctgacacaca atgtcccagc ctgccgataa gaggcattgt
    22681 tgcggccaca gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga
    22741 tcatcgaagg gattactgaa tgccatactc aactcctgtc atggaaaagt ggttgccaga
    22801 ggtcgattaa cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa
    22861 cctgacgcca gaaaatggat tcttttatag ggtgcaattg cgcatacagc gcctgattgg
    22921 cccgcatgat catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcgc
    22981 taacttcacc agcttttagc ttttcaagta acacgccctc ttgctgcccg tcccgatgcg
    23041 gccaccagta cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc
    23101 cggcatacag cgcggacagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc
    23161 gatcgctaaa aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc
    23221 agaaatccgc attacacggc agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca
    23281 acacatacac ggcgggagga agttgctgac gatgggtcag cgaagtcagc acaggccaga
    23341 cgggcatact ttgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttcttctg
    23401 ctgttacatc gccggtggta aaaatccata cacgacgtga atttttcaac cgttcacttt
    23461 tccagataat ttctttggct gctatttctg gcacttgcgg acaatcccat cccggttgca
    23521 gaggcgcttg cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt
    23581 gtggattcag cggatcggct attgcctggg gtaatagttt tcgccagcct tcacgtaatt
    23641 cgaggcgatc gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt
    23701 agctgccgcg ccagttggca ttgagttgtg tcgtccactg ccagacatca gtacctgcaa
    23761 ttcgctgcat cgactggggc tggctgttct gatgatgatc ggtcacacca gtgatgtaga
    23821 tccatacgcg ctttatcgtc gagtattctt cagaaccttg gggatcacgc caccagaaag
    23881 tgacctcaaa catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc
    23941 agctctcact tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt
    24001 ttcattgatt gcagaaatat attgataata ttattgataa ccatttgcat ttgcaatagc
    24061 gtaatggcgc gccgtgggaa gcgcggacat ttattaacca actgcactgc gtgtctttca
    24121 ggatcaaagg ttttcgcggt agcgggatgc gtcgtgttga ggacgaccat gcccgacagt
    24181 tgcaattcgt ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc
    24241 ttgttggtca atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct
    24301 gtttcacatg acgataccat tgtcgttacc gccgccgagc agaacttaca ggcgcctggc
    24361 gtttcgacca tcaccgcaga tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag
    24421 atcatccgta ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg
    24481 aataaccgac agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac
    24541 ggcaagccgg taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc
    24601 cgtggtgata cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt
    24661 ccggcagctg cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa
    24721 aaaggcagcg gtgagtggca cggctcctgg gacgcttatt tcaatgcacc agaacataaa
    24781 gaggaaggtg ccactaaacg caccaacttc agcctgaccg gtccgctggg cgacgaattc
    24841 agcttccgct tgtatggcaa cctcgacaaa acccaggctg acgcgtggga tatcaaccag
    24901 ggccatcagt ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg
    24961 gtgatcaaca aagatattaa tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg
    25021 gaactggaag caggttacag ccgccagggc aacctgtatg cgggcgacac ccagaacacc
    25081 aactccgatt cctatacccg ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag
    25141 aactactcgc tgacctggaa cggcggctgg gataacggag tgaccaccag caactgggtg
    25201 cagtatgaac acacccgtaa ctcgcgtatt ccggaaggtc tggcaggcgg taccgaaggg
    25261 aaatttaacg aaaaagcgac acaggatttc gtcgatatcg atcttgatga cgtgatgctg
    25321 cacagcgaag ttaacctgcc gattgatttc ctcgttaacc aaacgctgac gctgggtacg
    25381 gagtggaatc agcaacggat gaaggacttg agttccaata cccaggcgct gaccggggcg
    25441 aacaccggcg gcgctattga tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca
    25501 gaaattttct cgctgtttgc cgaaaacaac atggagctga ctgacagcac catcgtaacg
    25561 ccggggctgc gtttcgatca tcacagtatt gtcggcaata actggagccc ggcgctgaac
    25621 atatcgcaag gtttaggcga tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa
    25681 gcaccgagcc tgtaccagac taacccgaac tacattctct acagtaaagg tcagggctgc
    25741 tatgccagcg cgggcggctg ctatcagcaa ggtaacgatg acctgaaagc agaaaccagc
    25801 atcaacaaag agattggtct ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg
    25861 ttccgtaacg attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca
    25921 gtcggcaccg atctctatca gtgggataac gtgccgaaag cagtggttga aggtctggaa
    25981 ggatcgttaa acgtaccagt tagcgaaatg gtgatgtgga ccaataacat cacttatatg
    26041 ctgaagagtg aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtacacgttg
    26101 aactcaacgc tgagctggca ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg
    26161 tacggtaagc agcagccgaa aaaatacaac tacaaaggtc agccagcggt tggcccggaa
    26221 accaaagaaa tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag
    26281 aatgtcagtc tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt
    26341 aatgcccaga ccacgggcga tctggcaggg gccaactata tcgcgggtgc cggggcgtat
    26401 acctataacg agccgggacg tacgtggtac atgagcgtaa acactcactt ctgatgctaa
    26461 cgtcagattg ttgacaaagt gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc
    26521 ggcctacaaa aatcttgcca attcaatata ttgcaggacc atgtaggcct gataagcgta
    26581 gcgcatcagg cagttttgcg tttgtcatca gtctcgaata tggtcgatat gaaaactacg
    26641 catacctccc tcccctttgc cggacatacg ctgcattttg ttgagttcga tccggcgaat
    26701 ttttgtgagc aggatttact ctggctgccg cactacgcac aattgcaaca cgctggacgt
    26761 aaacgtaaaa cagagcattt agccggacgg atcgctgctg tttatgcctt acgggaatat
    26821 ggctataaat gtgtgcccgc aatcggcgag ctacgccaac ctgtctggcc tgcggaggta
    26881 tacggcagta ttagccactg tgggactacg gcattagccg tggtatctcg tcaaccgatt
    26941 ggcattgata tagaagaaat tttttctgta caaaccgcaa gagaattgac agacaatatt
    27001 attacaccag cagaacacga gcgactcgcg gactgtggtt tagccttttc tctggcgctg
    27061 acactggcat tttccgccaa agagagcgca tttaaggcat cggagagaca aactgaagca
    27121 ggttttctgg attatcagat aattagctgg aataaacagc aggtcatcat tcatcgtgag
    27181 aatgagatgt ttgctgtgca ctggcagata aaagaaaaaa tagtcataac tctgtgccaa
    27241 cacgattaat tgacaacatc tggtacgatt cgcccgcagc catcactgac tgcgggcgaa
    27301 agtgtaaagc aggtgcctta ccactctgac ctgacaaccg gatatgcagg gattgctccc
    27361 cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt
    27421 cctttacggt gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca
    27481 aacacagcac tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat
    27541 gcgaagaagg gactaccatc cgagttgttg aggttcggag tggcagcccc aggattgata
    27601 gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct
    27661 tcaggcaccc gccgttatct tacccttcct cagcaaaata tctgtttatt tatacagtaa
    27721 gtcctgtttc ggacttctgt tggcaacact cgttattgct cgtaaaacga aatcgcccgc
    27781 agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc ctaccatcct gacctgacaa
    27841 ccggatatgc ggggattact ccccgcacaa cggctacttc ttcggttcgt aagcgagaac
    27901 agccttaaac tcaatattac gttccttcac cgtaaactca cacagcgagt ctccgaccag
    27961 aagcgtaaat cccagcaccg tcaaacacag cactatgact gccgcaaggg catatttcgt
    28021 cagcatgttt atattgcctc catgcgaaga agggactacc atccaaattg ttcgggtttg
    28081 gagtggcagc cccagggttg atagaaatat ctcctggggc ttcatctttc cagacctcag
    28141 agtaaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt cctcaacaaa
    28201 atggctgttt atttatacag taaatcctgt ttcggacttc tcactcctca tttaatcaac
    28261 tgaagctgag cccgtttttt ctgcatgcct caatcgtcaa aattcttcgc catcagccag
    28321 ctctgccata acttgccaca ttgaaaaagt agccaaattg cttcagtcac catcatcaaa
    28381 aaaagaaaca ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata
    28441 gaaaacgcca acataagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt
    28501 taccctcggt attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca
    28561 ggactcttca atgctgattg acgcggttaa aaataagatc acggccggag aggtaaagca
    28621 cgatatcacc gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc
    28681 tgccgggcaa ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct
    28741 ggaaatttgc ggcggtggca cacacccgtt tcagaaatgg cagcgtcagg aggtatgcga
    28801 taacgagcgc tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt
    28861 ttttggtcag catgtccatg ttggctgcgc cagtggcgat gacgccattt atttgctgca
    28921 cggcttgtca cggtttgtgc cgcactttat cgccctttcc gccgcgtcgc catatatgca
    28981 aggaacggat acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcccgataa
    29041 tggcccgatg ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgtttgag
    29101 ttacaccacg atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca
    29161 ttttggcacg gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa
    29221 tatggcggga ttaattcagg ctaccgccca ctggttactg acagaacgcc cgttcaaaca
    29281 tcaggagaaa gattacctgc tgtataaatt caaccgtttc caggcctgtc gctatgggct
    29341 ggaaggcgtc attaccgatc cgcacactgg cgatcgtcga ccgctaacgg aagacacctt
    29401 gcgattgctg gaaaaaatcg ccccttccgc acataaaatt ggtgcatcga gcgcgattga
    29461 ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt
    29521 cgccgatggt ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccgatga
    29581 ctaagtaggg aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt
    29641 aacaatgtta tgaaacaccc tttagaaacc ttgactaccg cagcaggcat tttgctgatg
    29701 gctttcctct cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa
    29761 ctggtgacca tgttccatct gatggatctt agctagcttt acactttatt gttttgtctg
    29821 tggtttttag tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg
    29881 ttctcgctgg cggattaaac gtcgattatc gccgtacttc gcggcatact ttgcttattc
    29941 tccttcgcct aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct
    30001 ggcccttgag ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt
    30061 taaaattggc ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa
    30121 tctgaagtcc gatccacaaa aatccctgtt aaatcagcag atatacccga gcattaagcc
    30181 agggtatcac atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga
    30241 agcgttactt cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa
    30301 acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat ctatagcgca tttttctcgc
    30361 ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattacttt cacatggagt
    30421 ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag
    30481 taaaaaactt accccggaac aggccgagca gatcaaaact ctcctgcaat acagcccatc
    30541 cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg
    30601 tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac ttgatgcctc
    30661 gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt
    30721 tgaccaggaa gatgctgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa
    30781 aggtcgcaag ttcttcgctg atatgcaccg taaagatctg catgatgatg cagagtggat
    30841 ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg ctctgggtct
    30901 ggacgcggta cccatcgaag gttttgacgc cgccatcctc gatgcagaat ttggtctgaa
    30961 agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt
    31021 taacgctacg ctgccgaaat ctcgtctgcc gcaaaacatc accttaaccg aagtgtaatt
    31081 ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc
    31141 tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata
    31201 tcccaggttg aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt
    31261 ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg
    31321 cggaccttcg aaaaacgagc catcgccagt tcacggcttt ggttgcaaat cataacccag
    31381 aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg
    31441 gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg
    31501 tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg
    31561 gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa
    31621 ctgatcggcg cgatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca
    31681 gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat
    31741 ccgattcttg gtctggtggc cggtattcag ctttccgcga acgatatgct gaaactgggc
    31801 gactggctgg agatgccgaa atacggcgca gatggcgcgg tgatcgatat tgggttaacc
    31861 accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt
    31921 tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc
    31981 agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg
    32041 aataaagcgc atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg
    32101 aatcgccagc aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga
    32161 acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg
    32221 accttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg
    32281 tttactaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac
    32341 atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat
    32401 attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga
    32461 tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt
    32521 taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat
    32581 catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcag agatagtttc
    32641 cgaacggata gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgctgcac
    32701 gaaaacgcag atgcgccaga cagatcataa tccagttcaa cagcagcgtt gctaccacca
    32761 gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg
    32821 aagtgatcgc tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag
    32881 tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt
    32941 aaaccccgct gttatacact gacagcgatg ctaccagaat gacgaagttc agcgcagaag
    33001 ctaccacgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca
    33061 cttccaccca cggatagagc gccagtaaaa ccaccagtga accgatgtaa aacagcagga
    33121 tgcgatacac cacctgattt accgcttttg gaatgctttt atccggatcg cgcgcttcag
    33181 cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca
    33241 aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac
    33301 tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga
    33361 tgattgccag cactttaatc aacgcaaacc agaactcggt ttcgccatat aagcgcacgt
    33421 tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa
    33481 catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca
    33541 gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt
    33601 tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccaagct
    33661 ggcgcataat caggaaagcg atgatcccgg cgacgccgta acccagcaat acagccggac
    33721 ccgccatctg aatcgccgga ccaatgccaa gaaacagacc agtaccaatt gcgccaccca
    33781 gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg
    33841 acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt
    33901 tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt
    33961 aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata
    34021 cgccgccggg ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg
    34081 tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag
    34141 accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagta ccgcgccgtg
    34201 atacaacgcc tcatccagct tctgctcact gaatgtttgc ggattattca acgacggctc
    34261 ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgacccc
    34321 ggcaagggca ataaaaccgg tgcccgttgc cacagaaaga tgaaagccca tccaccacag
    34381 caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc
    34441 cacccgacgg aatgccagat acaacagcac gaagatgatc atcagtgtca tcggcaccat
    34501 gagtttcagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct
    34561 ggtgcccggt tttaactgca ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga
    34621 caccatgtca cgatcgcggg catcaatata aatccagctc gtcgggcgcg cattctcggt
    34681 tttcagcatc gacggtccgg tagagacttt aacgtcggcc acgtctgcca gggtgatttg
    34741 ctgcttcatc ggcgtcagga tcggtagctg gcgcagtgcc tgcgggctat cgcgccagct
    34801 ttgcggataa cgcagattaa tgggataacg ggcaatccct tccaccgttt cgccaaccat
    34861 cgccccgccc accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg
    34921 cgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gacgctcggc
    34981 tagagcagaa gccacgcttg gcaccgttcg cgctacttct tcaatttgct cagccatcgc
    35041 gtcgatatcc gccagcacag tgccggaaac tttaatgccg atggggcttt taatgccggt
    35101 tgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag
    35161 ccgcacggtg ttatccagtt cctcaatgat tttgtccatc gtcatgccag gccgccactg
    35221 atcctgcggc ttaagctgga tggtcgtttc aaccatctcg agcggagcag aatcggtggc
    35281 ggtttccgct ttcccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag
    35341 cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga
    35401 tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt
    35461 gagcggccag agaaccgtca gcaccgaaag cgctgccacc aacagcgtgg ttttcggcca
    35521 gtgcagtact ttcagcaata gaggatgata aacacgaatc aaaaagcgat tgagcgggtt
    35581 actgctttcc ggcggaattt tgccacggat ccagtacccc atcaggatag ggatcactac
    35641 gatcgccagc agcgccgcgc ccgccatcgc ataggtttta gtgaacgcca acgggccaaa
    35701 cagacgccct tcctgccctt ccagggtgaa gatcgggata aacgacaacg tgataatcag
    35761 cagactgata aatagcgccg gacccacttc aacagatgca tcggtgatca cctgccagcg
    35821 cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg
    35881 tgcattttcg atcatgacga tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc
    35941 gcccaacgac ataatattgg cattcagtcc ctggaagtgc atgacaataa aagcaataca
    36001 caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag
    36061 cgcacagact accgccacca cgataaactc ttccagcaac ttgccgctga ggttgtcgat
    36121 ggcgcggtca atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag
    36181 actacttttc agcgtttcca gtttgtcctt cacggaggcg atcacttctc gggcgttttt
    36241 gccggagcgc aggatcacca ccccgcccgc cacttcgcct tcgccattca gttcggcaat
    36301 gccccggcgc atctccgggc caatctggac cttcgcaaca tcacgcagat aaacgggcac
    36361 gccattttct ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc
    36421 gctggcgcgc accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg
    36481 gtttgaagca tccagcgcgc tttttacttc ggctagactg atgccatatt gcgccaggcg
    36541 ctggggatcg ataaccacct gatactcttt caccacgccg cccaccgacg ccacttccgc
    36601 aacgtcaggg atggttttta gctcatattt gagaaaccag tcctgcaatg agcgtaaatc
    36661 ggccagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
    36721 cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt
    36781 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtaccatctt cgaaaatgac
    36841 atacacataa gagtctccaa actgcgagaa accgcgcaca gtcttcgcgc caggcaccga
    36901 caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg gtgcctgacc
    36961 gggatagctg gttttaataa tcacctgcac atcggagaga tccggcagcg catccactgg
    37021 cgtgttaatg atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa
    37081 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
    37141 gcattggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
    37201 tcaatcagga acaggccgct ggaaaccacc ttttcacctt ccgccagacc agagtgtaac
    37261 gcggtgacgc cttgcgatgc ctggaaaacg gcaacgcgtt tcggtacaaa gcgtccgtcg
    37321 gcatcaacgg taatcacccg ctgttcgttg ccggtatcaa tcagcgcctg tgacggaatg
    37381 agcagcatcg gttcgctggc ggtgttgagt tgcaaccagg cgttcatgcc cggttttagc
    37441 gcctcgtcgg cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg
    37501 ccaggtagca gcgtccattt gcggatggtg agtgttttat ccggtcgcgc cggaacggtg
    37561 agcgtaaact gcgaggcatc tttcaccagc caggcgatag actccgggat cgcagcagtg
    37621 acccacaccg ggtccatacc ctgaattttc gctaccacgt tatctttggc gatattcatt
    37681 cccgcgcgca gatcaaacgc ggtgatcacg ccatcaatgg gtgctttgag cgtaaagcga
    37741 gtctggattt tttgcgtggc gatcaggcgg cgaatatccg cctccggcat tcccgccagt
    37801 cgcagccgct caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt
    37861 aaatactcac tctgcgcttc cacccagtca ggaatggtca ggtcgagaag cggtgtgccc
    37921 ttttgtactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga
    37981 gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca
    38041 aaagtcagcg gtccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc
    38101 gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc
    38161 atatccataa acggcgattt acctggttta tcgaaccgcg tattggggta catcgggtcg
    38221 taccagaata agattttacg ttctgcggtc gacgtttttt ctgcgggtgg ttcagccttt
    38281 gcaaaccagg taaaacctgc cgcagaaata ataccgcccg cgatcatgct gccgataata
    38341 agcgcgattt ttttcatctc attaaacctg ggttactggc tgactttaat atcctgtaat
    38401 aaagaaaggt tgccctgctg gacaaaatta aacgccactt tgtcgccggt tttaatttca
    38461 ctcattttcg tctgcggggt gatggtaaag cgcatagtca tctccggcca gttcacggca
    38521 gcaatcggat cgtgatggat ggtgattttt ttgctttcca gatcaatacc ctttaccacg
    38581 ccagtggcgc taataacctg cggttgtact tcgctcatgg tttcatgatg atgttcgtta
    38641 gcctgggcat taaagccaat aacggtaaac agactgaaca ttgcgacttg cagtgctttt
    38701 ttcatttatt ctctcctgga gttaaaaatt attgctgcca accgccccct aaggcggtat
    38761 acaaagaaat ttcgttaacc tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg
    38821 caaataaaga acgctcggca tccagcactt ccagataact taccgcgccg tgctgatata
    38881 atgcccgcgc ccgttgcaaa gtaatttgca gcgacgccag ataacgctgc tgggcgctga
    38941 tttgatcgtt caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct
    39001 ggattttctg ttcataattc actaccgact gctgctggcg aatttcggcg atatccagat
    39061 tggcctggtt gcgtccggca ttaaaaatgg ggatctcaat tttaggaata aaattccaca
    39121 tcccgctgct agcgttaaat aatgaagaca gatcgctgct ggcggttgat attccgctgg
    39181 tcaggctgat agacgggaaa aatgccgcac gcgcagcacc aatattagca ttagccgcca
    39241 ttaacgcgtg ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaagc
    39301 ccgccggtaa tttaacgctt tgcaggctgc cgctgtttac tgtctgcgct tgcggcagtt
    39361 tgccgtagct tcccaataac agttgcaatg cattattcgc ctgcgccagt tccccctgac
    39421 gtttagcgat gtcactgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa
    39481 cattgctgct accggtcaac agttgttttt cgacaaacgc atatgactgc tgataattac
    39541 gcagcgtttc ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt
    39601 gcgcgacatt agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat
    39661 aattttgtcg ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc
    39721 tggcgttaag gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc
    39781 tccagctgcc gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat
    39841 attgcgcccg tgcctcctgc actttcagcg tcgccatgcg caaatcccgg ttattcacca
    39901 gcgcctcgct aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccacgt
    39961 tctggtagtt atctgctgcg ttaaccaagc cgttctggct gagtgagaac tgctgcggca
    40021 cgggcattgc cgtacgctga taatccggtg ccagtgaaca accggttagc gcaagggcca
    40081 cacaaaatgg cagaagttta caaggagaca taggctcata atttctggtg attttatacc
    40141 gccaacttta ctcgccaggc tctgattttc cggtgacagg aaaatgacaa aattgtcatt
    40201 ttgccaataa gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg
    40261 aggaaaaatg aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa
    40321 agggttaacc gaagccggtt ttgtggtcga tttggccgac aacgggctga atggctacca
    40381 tctggcgatg accggtgatt atgacctgat aatcctcgat attatgctgc cggacgtgaa
    40441 cggctgggat atcgtgcgca tgttgcgctc cgccaataaa gggatgccga ttctgttgct
    40501 taccgcgctt ggcaccattg aacatcgcgt caaggggctg gagttggggg ccgatgacta
    40561 tctggtgaag ccgttcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg
    40621 cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt
    40681 cagccgcaaa gtcacccgca gcggcacgcg catcactctg accagtaaag agtttactct
    40741 gctggagttt tttctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca
    40801 ggtgtgggac atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct
    40861 acgcggcaaa atcgacaacg actttgagcc gaagctgatt cagaccgtgc gcggcgtggg
    40921 ctatatgctt gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa
    40981 cccgtctgac cttttttatc agcctggcca ccatcgcggc gtttttcgcc tttgcatgga
    41041 tcatgatcca ctcagtgaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa
    41101 ttagcgccac ccttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa
    41161 tgacgctgga agatatcgtc agtggttatt ccaacgtgtt gatttccctg gcagatagcc
    41221 acggcaaaac ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg
    41281 ccatacccga taaagacgct cagggtggcg aggtgtatct cctttccggc ccgacgatta
    41341 tgatgccagg ccacggtcac gggcatatgg aacacagcaa ctggcggatg attaacttgc
    41401 cggttggccc gttggtggac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg
    41461 attttcatct tcattacata aatgatttga tgaataaact tattatgacc gcatctgtca
    41521 tcagcatcct gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc
    41581 gcagcgtcag ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc
    41641 cgcagaccga gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc
    41701 gtattgagga tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc
    41761 gcacgccgat taccaatctc attacgcaaa cggaaatcgc cctcagccag tctcgcagcc
    41821 agaaggagct ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa
    41881 tggtcagcga tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga
    41941 aaatgctcaa cctggcggat gaagtcggca aagtgttcga ttttttcgag gcgttagcgg
    42001 aagatcgcgg cgtggagttg cgatttgttg gcgacaaatg tcaggttgcg ggcgatccgc
    42061 tgatgctgcg tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccaacca
    42121 gagagacaat tgtagtgcgc tgtcagacgg tcgatcacca ggtgcaagtt accgtcgaaa
    42181 accccggtac gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctaccgcg
    42241 ttgacccttc ccgccagcga aaaggcgaag gtagcggcat tgggctggcg atagtgaaat
    42301 cgattgttgt cgcgcataaa ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt
    42361 ttgtgattat gctgccggag agagagtgat tattaagttc aaattcaggg cgtggtgcat
    42421 cacctcctgg tattgttgat caacaaatta gagaaaaact tattaaacat atgaaatatc
    42481 gaaggctttt atttttcaaa gtttcacgag atgtttgtat atgaaactta gttaaatctg
    42541 tgtttcttcc aattgtagat cacaatgccc tttctaaatt ttcatcgtta taaacaagcc
    42601 atctatcata taaagcaatg aataatttaa tcgcgattaa tcatcattaa ttgttatatt
    42661 attcactcaa tctatttgtc aagtgaattt gataaattcc taatttaaaa tatccactat
    42721 tgagccagta tttaacaacg ttaagatgtg caatgtgcag atcatgttat cagggtggag
    42781 cggtagcata tatttcttat gcaatcattc attttaataa tgttaattgg attatttatt
    42841 gctgtgaata aaaatatatt atgaatattg gtttaacttt gcgtttttgt tactcgttgt
    42901 gatatgcgta cctgtcctga ataacgccaa gaaacaagag gttgagcttg tgattatttt
    42961 tttgactgaa cagacacccg tttctgtaaa gtaatattta aacaaaattc tcacataacc
    43021 atagcatttt ctgttccttc cctaataacg tctgaagtcg tcccgcttat cacattatcc
    43081 gcagttgtac ggtacggatc attattcatt ttactggagg ttgctatgtc aaccggatta
    43141 cgtttcacac tggaagtgga cggcctgccg ccggacgctt ttgcggtggt ttcctttcat
    43201 ctgaaccagt cactctcttc gctcttttcc ctcgatctct ccctggtcag ccagcagttt
    43261 ctctcccttg aatttgcgca ggtgctggac aaaatggcct acctgacgat atggcagggc
    43321 gatgaagtac agcgccgggt gaaaggcgtg gtgacctggt ttgaactcgg ggagaacgac
    43381 aaaaaccaga tgctgtacag catgaaggtg cacccgccgc tgtggcgtgc cggtctgcgc
    43441 cagaacttcc gtatcttcca gaacgaggac atcaaaagca tcctcggcac gatgttgcag
    43501 gaaaacgggg tgaccgaatg gagtccgctg ttcagcgagc cgcatccttc ccgtgagttt
    43561 tgtgtccagt acggtgagac tgattacgat ttcctgtgcc ggatggcggc ggaggaaggc
    43621 atcttctttt atgaggagca tgcttacaaa agtaccgacc agagcctggt gctgtgcgac
    43681 acagtccgcc atctgcccga atcttttgaa atcccatgga acccgaacac ccgtaccgag
    43741 gtgagcaccc tctgcatcag ccagttccgc tacagcgcac aaatccgccc ttcttccgtg
    43801 gtgaccaaag actacacctt taaacgcccc ggctgggccg gacgttttga acaggaaggc
    43861 cagcaccagg actaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag
    43921 ggtgctcacg ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caatgcagaa
    43981 accgcgcggg gaatgagtcg ctcgccggag atatggccgg gacgacgaat tgtgctgacg
    44041 gggcatccgc aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc
    44101 gaacagccac aggcggtgcc aggacggcag ggagcgggga cggcgctgga gaaccatttt
    44161 gcggtgatcc cggcagacag aacatggcga ccacagccgt tgctgaaacc gctggtcgac
    44221 ggcccgcaga gcgctgtcgt gacaggaccg gcaggcgagg aaatcttctg cgacgaacat
    44281 ggtcgcgtgc gggtgaagtt caactgggac cgttataacc cggcagacca agacagttcg
    44341 tgctggatcc gtgtggcaca ggcgtgggca ggcaccggtt ttggccacct ggcgataccg
    44401 cgtgtgggtc aggaagtgat tgtagacttc ctcaacggcg atccggacca gccgatcatt
    44461 atggggcgca cctaccacca ggaaaaccgc ccccccggca gcctgccggg aacaaagacg
    44521 cagatgacca tccgctccaa aacgtacatg ggcagcggat ttaatgagct gaagtttgat
    44581 gatgcgacag ggagagaaca ggtctacatc cacgcgcaga agaacatgga taccgaagtg
    44641 ctcaacgacc gtaccaccac cgtaaaacac gatcaccgcg aaaccgtaaa aaatgaccag
    44701 acggtcacga tccaggaagg taaccgcctt cttacggtgg aaaaaggcca caagatcacc
    44761 ggagtactga aagggtcttt atctgaggat gtctttcagg acagaggcac gattgccggt
    44821 tcggtgcatg ttgacgctgt aaacaatggt ggcgaaggcg acggtataca ggcttatacg
    44881 gcgattaagg aaattttgct ggccgtggag gaaagcaaaa ttgcgctgac gccggatagc
    44941 attcagctac aggtcgggga atcgacggta atcaggctgt cgaaggatgg catcaccatc
    45001 gtgggcggtt ctgttttcat caactgattc actcagatta tcagggagta ttttatgcag
    45061 ttcactttta atgaaggcca cattcaactg ccgtctcaat ggcaggatca gtcaatgcag
    45121 gtcctggtgt ccacagataa cagtggcatc aacctggtca ttacccgcga agccgttcca
    45181 cagggaaccc tgacaccaga gctgtaccag gaaacgctgg cgctgtacca gggcaaactt
    45241 gatgggtaca ccgaacatgc ctgccgggaa atcactctgg cagaggcccc ggcctggctg
    45301 ctggattatt cctggaacgg tccggaagat gaggggaatc aggggcggat tagccagata
    45361 gccgtattcc agcggcgtgg agatacgctg ctgaccttca ctttcagcac ctccttatcc
    45421 ctgaagaaca gtcaaaagac gatgctgctg gaggtgataa agagcttcac cccgttgcca
    45481 ccggagaatg acatccaaaa ggaccagccg cgctgacata aacgagggat aacacatgag
    45541 tgaaggacca ggcgggccac agggagcgac cgcaggcggt acgctggcaa tgcgaatgct
    45601 gtcacagcag gcgatggtcg ccagccagat gaaacgggca gccaacgaca aagccattgc
    45661 acagatgttg gcatcaaaaa agtctggccc ccccgccgcc aggctgggcg atgaaattca
    45721 gcacaagagt tttttggggg cgctggcagg ggccgtactg ggggcgatag tgaccatcgc
    45781 agaaggttgc ctgattatgg ccgcctgtgc caccggccct tatgcgctgg ttctggtacc
    45841 tgcgctgatg tatgccagct ataaggcgag tgattatgtg gaggagaaac agaaccagct
    45901 tgaatcatgg ataaacagct tttgtgacac ggacggcgcc atcaataccg gttctgaaaa
    45961 cgtaaaaatt aacggcgagc tggccgcacg tgcagcggtc acccttcccc ctcctccccc
    46021 acctggagca atacctgaag tcccacaggg ggaaccctca tggggtgata ttgccactga
    46081 cctgcttgaa tcggcagcgg aaaaagcagt accactggcg aaggcctggg ggaacgctgt
    46141 tatcaccctg acggacagca atgccggttt tatggatcgc gtcagcgccg gcgcatcgct
    46201 tctgtttccc gccggtccgg tattaatgga gtttgccacc atggtgggcg ggcgtggcga
    46261 aatcaaaaaa gatgtggatt tcccggaagc cggtgaggac acggcgctct gcgacaagga
    46321 gaacaaacca ccgaggatag cccagggcag cagcaacgtc tttatcaaca atcagcctgc
    46381 cgcgcgcaag ggcgacaaac tggagtgcag tgcggcgatc gtgggaggtt cgccggacgt
    46441 ctttattggc ggtgagcagg tcacatatct ggatatccag ccggagttcc cgccgtggca
    46501 gagaatgatc ctggggggaa taacgatagc cagctatctt ctgccgccag cgggactact
    46561 gggaaaactg aagaatctgg cgagactcgg caaactggga aactgctggg gaaaagcggg
    46621 aagctgctgg gcgcaaagct cggcgcgttg ctggggaaaa caggtaagtc gttaaaaagt
    46681 attgccaata aagtcatcag atgggtaaca gatcctgtcg atccggtaac cggcgcatac
    46741 tgcgacgaac gtaccgactt caccctgggc cagaccctcc ccctctcctt cacccgtttc
    46801 cacagttctg tactgccgct gcatggcctg accggcgtgg gctggagcga ctcctggagc
    46861 gaatacgcct gggtgcgtga acagggaaac cgggtggata tcatcagcca gggagccacg
    46921 ctgagatttg ccttcgacgg tgacagtgat acggcggtta acccgtatca cgcccagtac
    46981 attctgcgcc gccgcgatga ttatctggag ctgttcgaca gggatgcact gagcagccgc
    47041 ttcttttatg acgcctttcc gggaatgcgt ctgcgccacc cggtgactga cgataccagc
    47101 gatgaccgcc tggcacacag ccccaatgac cggatgtaca tgctgggcgg gatgagcgac
    47161 accgccagca accgcatcac gtttgagcgc gacagccagt accggatcac gggtgtcagt
    47221 cacaccgacg ggatccggct taaactgacg taccacgcca gcggctacct gaaagccatt
    47281 caccgcacgg ataacgacat acagacgctg gcgacctacg aacaggatgc gcgggggcgg
    47341 ctgacagaag cggatgcgcg gctggactac cacctgtttt atgagtacga cgctgcggac
    47401 cggatcatcc gctggtccga taacgaccag acgtggagcc gtttcaccta cgatgaacag
    47461 ggccggtgcg tgaatgtcac cggggcggag ggctattaca acgccacgct ggactatggt
    47521 gacggctgca ccaccgtgac ggacggcaag ggcattcacc gttattacta tgatcctgac
    47581 ggcaatattc tgcgggaaga agcgccggac ggcagcacca ccatgtatga atgggatgaa
    47641 ttccatcacc tgctggcccg ccactcccct gccgggcggg tggagaaatt tgaatacaac
    47701 gccgcacacg gtcagttaag ccgttacacg gcggcagacg gcgcggagtg gcagtaccgc
    47761 tatgatgagc gcggcctgct cagcaacatc accgaccctg ccgggcagac gtggacacag
    47821 cagtgtgatg aacgcggcct gccggtaagt ctggtgtcgc cacagggcga agagacccgg
    47881 ctggcataca ccgctcaggg gctgctgtcg ggaatattcc gccaggatga acggcgtctg
    47941 ggcatagagt acgaccacca caaccggccg gaaacactca ccgacgtgat gggccgtgaa
    48001 caccacaccg aatacagcgg tcacgacctg ccagtgaaga tgcgcggccc cggcggtcag
    48061 tcagtgcggt tacagtggca gcagcaccat aaactgagcg gcattgaacg tgctggaacc
    48121 ggcgcggaag gatttcgcta tgaccgccac ggcaacctgc tggcctggac ggacggcaac
    48181 ggtgttgtct ggacgatgga atacggtccg ttcgattttc cggtggcgcg aacggacggt
    48241 gaaggccacc gctggcagta ccgctacgat aaagacacgc tgcaactgac agaagtcatc
    48301 aacccgcagg gtgagtcata tctttatgtt ctggataact gcggccgggt gacggaagag
    48361 cgtgactggg gcggcgtggt ctggcgttac cgctatgacg ccgatggcct ctgtaccgcc
    48421 aaggttaacg gcctggagga aaccatcctc tacagccggg acgccgcagg ccgcctggca
    48481 gaaatcatct cgccggaagg caaaacgcag tatgcctatg acaaatccgg caggctgacg
    48541 ggtatcttca gcccggacgg cacatcacag cgcaccggct atgacgaacg cgggcgggtg
    48601 aatgtcacca ctcagggccg acgggccatt gaatacaact accccgatga acacaccgtc
    48661 atccgctgta tcctgccacc ggaagatgaa cgcgacagac accccggtga atccctgctg
    48721 aaaaccacgt accgctacaa cgccgccgga gaactgacag aggtcattct gccgggggat
    48781 gagacgctga cgttcagccg tgatgaggcg ggacgtgaag tgctccggca cagtaaccgg
    48841 ggttttgcct gtgaacaggg ctggaatgca gccggtcagc ctgtcagtca gcgcgccgga
    48901 ttttttccgg cagaagccac atgggacggg ctggttccct cactggtacg ggagtaccgt
    48961 tacgacagcg cgggtaacgt gtcaggcgtc accagccggg aagattacgg acgggaaaca
    49021 cggcgggagt atcggctgga ccggaacggc caggtcacgg cggtgacagc ctcaggcacc
    49081 gggctgggct atggcgaagg cgatgagtcc tatggctatg acagctgcgg ctacctgaag
    49141 gcgcagtctg cgggcaggca ccggataagt gaagagactg accagtatgc cggaggccac
    49201 cggctgaaac aggccggaaa cacgcagtat gactatgacg ccgcaggccg gatggtcagc
    49261 cgcaccagac accgtgacgg ctaccgaccg gaaacagagc ggttccggtg ggacagccgg
    49321 gaccagctga ccgggtattg cagcgcacag ggtgagctgt gggaataccg ccacgacgcc
    49381 agcggcagac gaacagagaa acgctgcgac cggaagaaaa tccgcttcac gtatctgtgg
    49441 gacggcgaca gtattgcgga aatccgggaa taccgcgatg ataaactgta cagcgtaagg
    49501 cacctggtgt ttaacggctt tgagctgata agccagcagt tcagccgggt acggcaggcg
    49561 catccgtcgg tggccccgca gtgggtgacg cggacgaatc atgcggtgag cgacct
//
LOCUS       DAJTVJ010000014        46686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000014 DAJTVJ010000000
VERSION     DAJTVJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.014"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..136)
                     /gene="tabA"
                     /locus_tag="OO448_000783"
     CDS             complement(<1..136)
                     /gene="tabA"
                     /locus_tag="OO448_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6336078.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(281..874)
                     /locus_tag="OO448_000784"
     CDS             complement(281..874)
                     /locus_tag="OO448_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6336079.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            945..1658
                     /gene="bdcA"
                     /locus_tag="OO448_000785"
     CDS             945..1658
                     /gene="bdcA"
                     /locus_tag="OO448_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6336080.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1789..2184
                     /locus_tag="OO448_000786"
     CDS             1789..2184
                     /locus_tag="OO448_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6336081.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2465..2599
                     /gene="pyrL"
                     /locus_tag="OO448_000787"
     CDS             2465..2599
                     /gene="pyrL"
                     /locus_tag="OO448_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6336082.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2603..3538
                     /gene="pyrB"
                     /locus_tag="OO448_000788"
     CDS             2603..3538
                     /gene="pyrB"
                     /locus_tag="OO448_000788"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6336083.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3551..4012
                     /gene="pyrI"
                     /locus_tag="OO448_000789"
     CDS             3551..4012
                     /gene="pyrI"
                     /locus_tag="OO448_000789"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6336084.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4085..4471
                     /gene="ridA"
                     /locus_tag="OO448_000790"
     CDS             4085..4471
                     /gene="ridA"
                     /locus_tag="OO448_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6336085.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4677..7373)
                     /gene="mgtA"
                     /locus_tag="OO448_000791"
     CDS             complement(4677..7373)
                     /gene="mgtA"
                     /locus_tag="OO448_000791"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6336086.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7514..7567)
                     /gene="mgtL"
                     /locus_tag="OO448_000792"
     CDS             complement(7514..7567)
                     /gene="mgtL"
                     /locus_tag="OO448_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6336087.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7752..8699
                     /gene="treR"
                     /locus_tag="OO448_000793"
     CDS             7752..8699
                     /gene="treR"
                     /locus_tag="OO448_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6336088.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8818..10239
                     /gene="treB"
                     /locus_tag="OO448_000794"
     CDS             8818..10239
                     /gene="treB"
                     /locus_tag="OO448_000794"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6336089.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10289..11944
                     /gene="treC"
                     /locus_tag="OO448_000795"
     CDS             10289..11944
                     /gene="treC"
                     /locus_tag="OO448_000795"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6336090.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVQHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12338..14476
                     /gene="nrdD"
                     /locus_tag="OO448_000796"
     CDS             12338..14476
                     /gene="nrdD"
                     /locus_tag="OO448_000796"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6336091.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14634..15098
                     /gene="nrdG"
                     /locus_tag="OO448_000797"
     CDS             14634..15098
                     /gene="nrdG"
                     /locus_tag="OO448_000797"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6336092.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15143..15529)
                     /gene="cybC"
                     /locus_tag="OO448_000798"
     CDS             complement(15143..15529)
                     /gene="cybC"
                     /locus_tag="OO448_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6336093.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15713..17065)
                     /gene="pmbA"
                     /locus_tag="OO448_000799"
     CDS             complement(15713..17065)
                     /gene="pmbA"
                     /locus_tag="OO448_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6336094.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17159..17710
                     /locus_tag="OO448_000800"
     CDS             17159..17710
                     /locus_tag="OO448_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6336095.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17793..19166)
                     /gene="mpl"
                     /locus_tag="OO448_000801"
     CDS             complement(17793..19166)
                     /gene="mpl"
                     /locus_tag="OO448_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6336096.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19342..20340
                     /gene="fbp"
                     /locus_tag="OO448_000802"
     CDS             19342..20340
                     /gene="fbp"
                     /locus_tag="OO448_000802"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6336097.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20373..21368)
                     /gene="yjfF"
                     /locus_tag="OO448_000803"
     CDS             complement(20373..21368)
                     /gene="yjfF"
                     /locus_tag="OO448_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6336098.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21355..22377)
                     /locus_tag="OO448_000804"
     CDS             complement(21355..22377)
                     /locus_tag="OO448_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6336099.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22391..23893)
                     /locus_tag="OO448_000805"
     CDS             complement(22391..23893)
                     /locus_tag="OO448_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6336100.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24033..24989)
                     /gene="ytfQ"
                     /locus_tag="OO448_000806"
     CDS             complement(24033..24989)
                     /gene="ytfQ"
                     /locus_tag="OO448_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR6336101.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25299..25829
                     /gene="ppa"
                     /locus_tag="OO448_000807"
     CDS             25299..25829
                     /gene="ppa"
                     /locus_tag="OO448_000807"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6336102.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26208..26549)
                     /locus_tag="OO448_000808"
     CDS             complement(26208..26549)
                     /locus_tag="OO448_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6336103.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26552..30331)
                     /gene="tamB"
                     /locus_tag="OO448_000809"
     CDS             complement(26552..30331)
                     /gene="tamB"
                     /locus_tag="OO448_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6336104.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30328..32061)
                     /gene="tamA"
                     /locus_tag="OO448_000810"
     CDS             complement(30328..32061)
                     /gene="tamA"
                     /locus_tag="OO448_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6336105.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32267..32905
                     /gene="msrA"
                     /locus_tag="OO448_000811"
     CDS             32267..32905
                     /gene="msrA"
                     /locus_tag="OO448_000811"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6336106.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33228..34571
                     /locus_tag="OO448_000812"
     CDS             33228..34571
                     /locus_tag="OO448_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6336107.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34651..34857)
                     /locus_tag="OO448_000813"
     CDS             complement(34651..34857)
                     /locus_tag="OO448_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6336108.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35182..35736
                     /locus_tag="OO448_000814"
     CDS             35182..35736
                     /locus_tag="OO448_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6336109.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35826..36737)
                     /locus_tag="OO448_000815"
     CDS             complement(35826..36737)
                     /locus_tag="OO448_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336110.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36949..37689)
                     /gene="cysQ"
                     /locus_tag="OO448_000816"
     CDS             complement(36949..37689)
                     /gene="cysQ"
                     /locus_tag="OO448_000816"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6336111.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37879..39822
                     /gene="cpdB"
                     /locus_tag="OO448_000817"
     CDS             37879..39822
                     /gene="cpdB"
                     /locus_tag="OO448_000817"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6336112.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39940..40320)
                     /locus_tag="OO448_000818"
     CDS             complement(39940..40320)
                     /locus_tag="OO448_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6336113.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40409..41269
                     /gene="qorB"
                     /locus_tag="OO448_000819"
     CDS             40409..41269
                     /gene="qorB"
                     /locus_tag="OO448_000819"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6336114.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41377..42342
                     /locus_tag="OO448_000820"
     CDS             41377..42342
                     /locus_tag="OO448_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6336115.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42450..43112
                     /gene="ytfE"
                     /locus_tag="OO448_000821"
     CDS             42450..43112
                     /gene="ytfE"
                     /locus_tag="OO448_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6336116.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43157..44569)
                     /gene="cycA"
                     /locus_tag="OO448_000822"
                     /gene_synonym="dagA"
     CDS             complement(43157..44569)
                     /gene="cycA"
                     /locus_tag="OO448_000822"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6336117.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44878..45498)
                     /gene="fklB"
                     /locus_tag="OO448_000823"
     CDS             complement(44878..45498)
                     /gene="fklB"
                     /locus_tag="OO448_000823"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6336118.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45717..46355
                     /gene="ytfB"
                     /locus_tag="OO448_000824"
     CDS             45717..46355
                     /gene="ytfB"
                     /locus_tag="OO448_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR6336119.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46339..>46686)
                     /locus_tag="OO448_000825"
     CDS             complement(46339..>46686)
                     /locus_tag="OO448_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6336120.1"
                     /translation="NPWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGK
                     KHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETIL
                     SWLLVDPSSTAHE"
BASE COUNT        11456 a        12415 c        11632 g        11183 t
ORIGIN      
        1 aaaacagacg attgccttcg atatcgtgct tgccctttgg cgtttctgcc gtaacgtgtg
       61 ctttgatatg ctcaatcgcc aggcgtaact cctggggtag ccacggctga agattatgaa
      121 tatttccgat gatcatcttt tccactcctt aattaaaaca atgtttcatt tccatcgtta
      181 tacgaaaaga tgatcaattc cgctagcctt aatgcgtgaa ttattgtctt atcacaactt
      241 atcggcaggc gcgttcccgc ctttcgggtc aaggcccgca tcactccttg agaatagttt
      301 ttatcgcttc ccccgccagt gcagctgttg cacagagttg ttcgatggag tgcccctccc
      361 gagccttcgc agaaagccct gacatcacgg tactcataaa atcagtcaca cattgtgcgc
      421 tttgtggatg ccgcctggcg atatagtcat aaatggccgt ttctgcggcg tgataatatt
      481 gaacggcaat atcacgcgct tgtggatcat gactatgaat gccttcaaga accatacagc
      541 cagcgcagcc gccgttttgg ctatatctgc gcgccgcttc ttttaatacc tcagccaggc
      601 actcccctac cggacgatca tcacgaagaa tatcggcaag cggaatagct tccgtaccga
      661 catattcatt gagtacacgg ctaaataacc cagccttact gccaaaagcc gcgtagaggc
      721 tcggggggtt aataccaaga taatcagtaa cttcagcaac actgacagca tcgaaacctt
      781 tttggtgaaa aagcactttt gccgcagaga ctgcctgctc aggagcgaaa cgtctgggac
      841 gacctggaac gcgagattgt tttttagtga ccattacaaa acttgttgac agaaagttaa
      901 aacagttttg taatgcatgt tacataataa atcaaggagt ccttatgggc gcttttacag
      961 gtaagacagt tctcatcctc ggtggcagtc gtggtatcgg tgccgctatc gtacgtcgtt
     1021 tcgtcaccga tggggccaat gtacgattca cctatgcggg gtcgaaagat gccgctgaac
     1081 acctggcaca aaagactgga gcgacagcag tattcacaga tagtgctgac agagacgctg
     1141 tcattgatgt cgttcgtaag agcggcgcat tggatatcct ggtggtaaat gcaggtattg
     1201 gcgtctttgg cgatgccctg gaattgaatg ccgacgatat tgatcgcctt ttcaaaatca
     1261 atattcatgc tccttatcat gcctctgttg aagccgcccg gcagatgccc gaaggcgggc
     1321 gcatcttaat catcggctcc gtgaatggcg atcgtatgcc tgttgcaggc atggctgctt
     1381 atgccgccag taaatctgcc ctgcaaggca tggcgcgcga gctggcccgt gatttcggac
     1441 cgcgtgggat caccatcaac gtcgtccagc cagggccaat tgataccgac gctaatcccg
     1501 ccaacgggcc aatgcgcgat atgttgcata gttttatggc tatcaaaaga catgggcaac
     1561 cggaagaggt cgctggtatg gtcgcatggt tagcagggcc agaagccagt tttgttaccg
     1621 gcgcgatgca taccattgat ggcgcgtttg gcgcataacc gactacgctc aattaagccc
     1681 agccatttcc catgatgtct gggttttgtt tactcacgtc gtccgctaaa agcggctcct
     1741 ggtaaatata aatcttctgt tcatgtcgta aatcttgagg ataaccaaat ggtagaaaga
     1801 accgctgttt tccctgcagg ccgacattca ctgtatgctg aacatcgtta ttctgcggct
     1861 attcgttccg gcgatttgct gtttgtttcc gggcaagtag gaagtcgaga ggacggaaca
     1921 ccagaacccg attttcagca acaagtcaga ctggcatttg ataatttgca tgcgaccctg
     1981 gctgctgcgg gatgcacttt tgacgatatc attgatgtta cgagcttcca taccgatcca
     2041 gaaaaccaat ttgaagacat catgacggtg aaaaatgaaa tatttagcgc cccaccttat
     2101 ccaaactgga cggcggtagg tgttacatgg ctggcaggct ttgattttga aattaaagtg
     2161 atagcgcgca tccctgagca gtaagcaata gtgttagccg ttcgttttca cactccgccc
     2221 tataagtcgg atgaatggaa taaaatgcat atctgattgc gtgaaagtga aaaaggaaaa
     2281 agcaggaaat gtctgcaatt attgataccg aacgaccgtt cccctgcaga atcacaacaa
     2341 ataaaaatgc atgtattttg acttataatt caaataaacc gtttgcgctg acaaaatatt
     2401 gcatcaaatg cttgcaccgc ttctgacgat gagtataatg ccggacaatt tgccgggagg
     2461 atgtatggtt cagtgtgttc gacattttgt cttaccgcgt ctgaaaaaag acgctggcct
     2521 gccgtttttc ttcccgttga tcacccattc ccagcccctc aatcgagggg cttttttttg
     2581 cccaggcgtc aggagataaa agatggctaa tccgctatat cagaaacata tcatttccat
     2641 aaacgacctt agtcgcgatg accttaatct ggtgctggcg acagcggcga aactgaaagc
     2701 aaacccgcaa ccagagctgt tgaagcacaa agtcattgcc agctgtttct tcgaagcctc
     2761 tacccgtacc cgcctctctt tcgaaacttc catgcaccgc ctgggtgcca gcgtggtggg
     2821 cttctccgac agcgccaata catcactggg taaaaagggc gaaacgctgg ccgataccat
     2881 ttcggttatc agcacttacg tcgatgcgat agtgatgcgt catccgcagg aaggtgcggc
     2941 gcgcctggcc accgagtttt ccggcaatgt accggtactg aatgccggtg atggctccaa
     3001 ccaacatccg acgcaaacct tgctggactt attcactatt caggaaaccc aggggcgtct
     3061 ggacaatctc cacgtcgcaa tggttggtga cctgaaatat ggccgcaccg ttcactccct
     3121 gactcaggcg ttagcgaagt tcgacggcaa ccgtttttac ttcatcgcgc cggacgcgct
     3181 ggcaatgccg caatacattc tggatatgct tgatgaaaaa gggatcgcct ggagtctgca
     3241 cagctctatt gaagaagtga tggcggaagt agacatcctg tacatgaccc gcgtgcaaaa
     3301 agagcgtctg gacccgtccg agtacgccaa cgtgaaagcg cagtttgttc ttcgcgccag
     3361 cgatctccac aacgccaaag ccaatatgaa agtgctgcat ccgctgccgc gtgttgatga
     3421 gattgcgacg gatgttgata aaacgccaca cgcctggtac ttccagcagg caggcaacgg
     3481 gattttcgct cgccaggcgt tactggcact ggttctgaat cgcgatctgg tactgtaagg
     3541 ggaaatagag atgacacacg ataataaatt gcaggttgaa gctattaaac gcggcacggt
     3601 aattgaccat atccccgccc agatcggttt taagctgttg agtctgttca agctgaccga
     3661 aacggatcag cgcatcacca ttggtctgaa cctgccttct ggcgagatgg gccgcaaaga
     3721 tctgatcaaa atcgaaaata cctttttgag tgaagatcag gtagatcaac tggcattgta
     3781 tgcgccgcaa gccacggtta accgtatcga caactatgaa gtggtgggta aatcacgccc
     3841 cagtctgccg gagcgcatcg acaatgtgct ggtctgcccg aacagcaact gtatcagcca
     3901 tgccgaaccg gtttcttcca gctttgccgt gcgaaaacgc gccaatgaca tcgcgctcaa
     3961 atgcaaatac tgtgaaaaag agttttccca taatgtggtg ctggccaatt aattgcggtt
     4021 ggtaataaaa gtctggctcc ctataatgag ccagactttt taccgctgta ataaaggaga
     4081 aatcatgagc aaaactatcg cgacggaaaa tgcaccggca gctatcggtc cttacgtaca
     4141 gggcgttgat ctgggcaata tgatcatcac ctccggtcag atcccggtaa atccgaaaac
     4201 gggcgaagta ccggcagacg tcgctgcaca ggcacgtcag tcgctggata acgtaaaagc
     4261 gatcgtcgaa gccgctggcc tgaaagtggg cgacatcgtt aaaaccaccg tgtttgtgaa
     4321 agatctgaac gacttcgcaa ccgttaatgc tacctacgaa gccttcttca ccgaacacaa
     4381 tgccaccttc ccggcacgtt cttgcgttga agttgcccgt ctgccgaaag acgtgaaaat
     4441 tgagatcgaa gcgatcgctg ttcgtcgcta atcttgatgg aaatccgggc tatcatgccc
     4501 ggattaagtc tgatgacaaa cacaaaatcg cctgatgcgc tacgcttatc aggcctacgt
     4561 gattcctgca atttattgaa tttgttggcc ggataaggca tttacgccgc atccggcatg
     4621 aacaaaactc actttgtctg caatctgaat ccaggccacc atgcccagtt gattctttat
     4681 tgccagccgt aacgacggct ataaaatcct ttcaccagtt gggttaacgt catataccct
     4741 gccagaatcg caaccagcca cgggaaatag cttaacggca gcgcctgcag ttgcagataa
     4801 ctggccagcg gtgaaaacgg caatgcgatc ccgacaatca tcacgatcac ggtcatgatc
     4861 attaacggcc acgatgcaca gctctgaata aacggcacac ggcgggtgcg gatcatatgc
     4921 acaatcagcg tttgcgacag caagcccacc acaaaccatc ccgactggaa cagcgtttgc
     4981 gtttccggcg tgttggcatg gaatacccac cacatcaggc aaaacgtcag aatatcgaag
     5041 atcgagctga tcggtccgaa gaagatcata aagcgcccca gatccgccgg attccaacgc
     5101 tgcggctttt gaatttgctc gtcgtcgacg ttatcaaacg ggatcgccac ctgtgacaca
     5161 tcatacagca ggttctgaat aagcaagtgt aacggcaaca tcggcaggaa gggcaagaaa
     5221 gcactcgcca ccagcacgct gaacacatta ccgaagttgg agctcgccgt cattttgatg
     5281 tatttcagca tgttggcgaa agtgcgacgt ccttcaataa ccccctcttc cagcaccatc
     5341 aggctttttt ccagcaggat gatatcagcc gcttcacggg caatatctac cgcgccgtcc
     5401 acagaaatgc cgatatccgc agcgcgtaag gcgggcgcat cattaatacc atcgcccata
     5461 aagccaacca catgcccttc gcgcttcagt aaggtcacaa tgcgttcttt atgcatcggc
     5521 gtcagacgag caaacagcgt ggtgcgctgt gcgagatttg ccagttcgtc gtcagatagc
     5581 gtttcaatat cactaccaat gaccacctct cccgcgtcca gccccacttc atggcacact
     5641 ttcgctgcaa ctaactcact gtcgccagta aggattttta cggtaattcc actcgctttt
     5701 aatgccttca gtgccggagc agttgtctct tttggcggat caaggaaagc aatatatcct
     5761 tcgaggatca ggtcggattc atccgcccgc tggtaatctc cttcacgcgc tggcaggtat
     5821 ttcgtcgcca ccgcaaccac gcgcagcccc tgacgattca gcgtatcagt aacccgctta
     5881 atcttacgca gcatgatgtc atcgagcggc acaatctcgc cattgtgacg cacctgcgaa
     5941 cacacattga ggatttcctg caatgcacct ttgcaaacca gctgatggtg ctcggtattt
     6001 tctgccacta ccaccgacat ccggcgacgc tcgaaatcaa acggaatctc atcaattttt
     6061 tgccaacggc tggccagaga gcgcgctgac tcttcatccg taccttcgag caccgctgta
     6121 tcgagtaggt ttttaagtcc ggtctgataa tgactgttca accacgcgct atgcagcacg
     6181 cgttcgctgg ttttaccgga gatatcggta tgattctcca gcacaatttt gtcctgtgtc
     6241 agggtgccag ttttatcagt gcacagaata tccattgcgc caaagttctg aatagcatcc
     6301 agatgtttga cgatcacttt ctgtttcgac agctttactg cgccgcgcgc cagcgtcgag
     6361 gtaacaatca tcggcaacat ttccggcgtt aagcctaccg cgaccgaaag cgcaaacagt
     6421 gccgcttccc accagtcgcc tttggtgtaa ccgttgataa gcaacaccac cggagccatc
     6481 accagcataa agcgaatcag cagcatactg acgcggctga tcccctgctg aaaggcattc
     6541 ggctcgcttt cctgctcact aacacgcccc gccagttgac caaaccaggt gttggctcct
     6601 gtagcaatca ccattgcttg tgccgtgccg ctcaccacgg tggtgcccat aaaacacagg
     6661 gtgtcacact ccagcggatt gctgtgctcc ggctggcgag tggtagcggc tttttccacg
     6721 ggcagagact caccggttaa cgacgcctga gcgacgaaca gatcccgcgc ctgcaagata
     6781 cgtaaatctg ccgggatcat atcccccgcc gccagtttta taatatcgcc gggcaccagc
     6841 tggtcgatcg ggatctccag ccagccattt tcgcctttgt cgttaattac gcgcagcacc
     6901 gtcgcggtat tgctgaccat cgctttcagg gcatctgccg ctttagtgga acgtgcttcc
     6961 tgaataaagt tcagcaacgt agaaatagcg accattagcg cgataacgcc cgcggcaaat
     7021 aaatcttcag tggcgtaaga aatagcgccg agaatggtga gtaaaatatt aaatgggttg
     7081 cgatagcaga cccataaatg tacccaccac ggcgacggtt gttgtgcggg taatttattt
     7141 tcaccatgtt gttcgcgggc agattccact tccgcctgat ttaacccttc cggatgggtg
     7201 tcgaacgttt tccacaattc ttcttcgggc atcaccgcca ttttcaggca atgcgcactt
     7261 aaggacggcg ggaccgccgt attcactgtc tgctgcgcgc caggcaatgg atcgcgatga
     7321 accagacggg aaggtaaatg gcgaatgagc cgggtaaaaa tttctttaaa cataaggagt
     7381 ccctccgcac tgtctgaaac agtacagaaa tgtcttcagg cgtggcgaag ccttacctga
     7441 aggtaataaa gctgagcagg gacgttaaaa cgtcacggcc ttacgcatcc ggtagcgccg
     7501 tgaaaagacg ggcttaccgg aaaagtttta atctccgtcg agggagaggc gtgggatcag
     7561 gttccatata acctccggta agtgaaaaaa gcgccgcaaa taataaggag aatccaataa
     7621 tattcgtagc atttatggcg atataatact tgctggcaac taaaccaacg ataaaccaga
     7681 ctttacccat tgctgaatgc acgggtaaag ttaggctcaa ataattaaac aacacgttac
     7741 aggacaacag gatgcaaaat cggctgacta tcaaagacat cgcacgctta agcggcgtgg
     7801 ggaaatctac agtttcccgg gtgctgaata acgaaagcgg cgtgagccag cgcacccgcg
     7861 agcgtgttga agcagtgatg aatcagcatg gattttcccc ttcccgctct gcgcgcgcta
     7921 tgcgtgggca aagcgataaa gtggtcgcca tcattgttac ccgtctggat tcgttgtcag
     7981 aaaatctcgc cgttcaaacc atgctgccag cgttctatga acaaggttac gacccaatca
     8041 tgatggaaag ccagttttcc ccgcaattag ttgccgaaca tttgggggtg ctgaaacggc
     8101 gtaatatcga cggcgtagtg ctgttcggtt ttactggcat aacagaagaa atgttagccc
     8161 actggcagtc atcgctggtt ctgctggcgc gtgacgcaaa aggctttgct tcggtctgtt
     8221 atgacgacga aggggcaatc aagatcctga tgcaacggct gtatgaccag gggcatcgta
     8281 atatcagtta tctcggcgtg ccgcatagtg acgtgacaac cggtaagcga cgtcacgaag
     8341 cctacctggc gttctgcaaa gcgcataaac tgcatcccgt tgccgctctg ccagggcttg
     8401 ctatgaagca aggctatgag aacgtggcaa aagtgattac gcctgaaact actgcgttac
     8461 tgtgcgcaac cgacacgctg gcacttggcg caagtaaata cctgcaagag caacgcatcg
     8521 acaccttgca actggcgagc gtcggtaata caccgttaat gaaattcctc catccggaga
     8581 tcgtcaccgt agatcccggt tacgccgaag ctggacgcca ggcggcctgc caattgatcg
     8641 cacaggtaac cgggcgcagc gaaccgcaac aaatcatcat ccccgccacc ctgtcctgat
     8701 cgtttcctga acgataaatt gtgatcttcg ctgcatttcg ggaacgttcc cgtttttaaa
     8761 tttttccgcg caatatattc tgcagccaac caaaaatgtc atctgccatg gggctttatg
     8821 atgagcaaaa taaaccaaac ggatatcgat cggttgattg aactggtcgg cgggcgcggc
     8881 aatattgcga cggtgagcca ctgtattact cgcctgcgct ttgtcctcaa ccaaccggcc
     8941 aatgccagac cgaaagaaat tgagcaactc cccatggtga aaggctgttt caccaatgcc
     9001 gggcaatttc aggtggtgat tggcaccaac gtgggtgatt actatcaagc actaatagcg
     9061 tcaaccggac aggcgcaggt tgataaagag caggtaaaaa aagccgcccg gcagaatatg
     9121 aaatggcatg agcagttgat ctctcatttc gcggagatct tcttcccgtt gctgcccgcg
     9181 ttgattagcg gcggtttgat cctcggtttt cgcaatgtga tcggcgattt gcccatgagc
     9241 aacggtcaga cgctggcgca aatgtaccct tccctgcaaa cgatctacga ttttctgtgg
     9301 ttgatcggtg aagcgatttt cttctacctg ccggtcggga tttgctggtc agcggtgaaa
     9361 aaaatgggcg gcacgccgat ccttggtatc gtgcttggcg tgacactggt ttccccccag
     9421 ctgatgaacg cttatctgct cgggcagcag ctgccggaag tgtgggactt tggcatgttc
     9481 agcatcgcca aagtgggcta tcaggcgcag gtgatcccgg cactgttagc cgggctggcg
     9541 ctgggcgtta ttgaaactcg ccttaaacgc atcgtaccgg attacctcta tctggtggtg
     9601 gtgcccgtct gttcgctgat cctcgcggtg ttcctcgccc atgcgctgat tggtccgttt
     9661 ggtcgcatga ttggcgatgg cgttgccttt gcggtacgtc acctgatgac cggcagcttt
     9721 gctccgattg gtgcggcatt gtttggcttc ctgtacgcgc cgctggtgat caccggcgta
     9781 caccagacca cccttgctat tgatttgcag atgattcaaa gcatgggtgg cacgccagtg
     9841 tggccgctga ttgcgctgtc gaatatcgct cagggctccg ccgtgatagg cattatcatt
     9901 tccagccgca agcacaatga acgcgagatc tccgtgcctg ccgctatctc cgcctggctt
     9961 ggggtcactg agcctgcaat gtacggcatc aacctgaaat atcgcttccc gatgctgtgc
    10021 gcgatgattg gttctggtct ggcaggattg ctatgcggcc tgaacggcgt tatggcgaat
    10081 ggtatcggcg tagggggcct gccgggaatt ctctcgattc aaccgagcta ctggcaggta
    10141 tttgcgctgg caatggctat cgccatcatc atcccgattg tactcacctc gtttatctat
    10201 cagcggaaat accgcttggg cacgctggac attgtttaat tttcttcggg gcgcaattgc
    10261 gccccctcgc atttgcagga ataacgtaat gactaatctt ccccactggt ggcaaaacgg
    10321 cgttatctac cagatttatc caaagagttt tcaggacacc acgggtagcg gcaccggcga
    10381 tttacgtggc gttatccaac gcctggacta tctgcataaa ctgggcgttg atgccatctg
    10441 gctaaccccc ttttatgtct ctccccaggt cgataacggt tacgatgtag cgaactatac
    10501 ggcgattgat cccacctacg gcacgctgga cgattttgac gaactggtga cgcaggcaaa
    10561 atcgcgcggg attcgtatca ttctcgatat ggtgtttaac catacctcta cccaacatgc
    10621 ctggtttcgc gaggcgctga acaaagaaag cccttaccgc cagttttata tctggcgcga
    10681 tggcgaacca gaaacgccac cgaacaactg gcgttcaaaa tttggcggta gtgcgtggcg
    10741 ctggcatgcg gaaagcgaac agtactattt gcatctcttt gcaccagaac aggcggatct
    10801 caactgggag aatccagcgg tacgcgcaga gctgaaaaaa gtctgtgaat tctgggccga
    10861 tcgtggggtc gacgggttgc gcctggatgt ggtgaatctg atctccaaag acccgcgttt
    10921 ccctgaagac ctggatggcg acgggcgtcg cttctacacc gacgggccac gagcacacga
    10981 gtttttgcac gagatgaacc gcgatgtgtt tacgccacgc gggttaatga ccgtaggtga
    11041 aatgtcctcc accagccttg agcattgcca gcgatacgcg gcactgacag gcagtgaatt
    11101 gtcgatgacc tttaattttc atcacctgaa ggtcgattat cccggtggtg aaaaatggac
    11161 gctggctaaa cctgactttg tggcgttgaa aacattgttc cgccactggc aacaaggaat
    11221 gcacaacgtg gcatggaatg ccttgttctg gtgtaaccac gatcagccgc gcattgtttc
    11281 tcgctttggt gatgaaggtg aataccgcgt gcctgcggca aaaatgctgg cgatggtgca
    11341 gcatggcatg cagggaacgc cgtatatcta ccagggcgaa gagattggca tgaccaaccc
    11401 gcatttcacg cgcattactg actatcgcga cgtggagagc ctcaatatgt ttgccgagct
    11461 gcgcaacgat ggtcgtgatg ccgacgagtt attggcaatc ctcgccagta aatcccgtga
    11521 caacagccgc acgcccatgc aatggagcaa cggcgataat gccgggttta cggctggcga
    11581 accgtggatt ggcctgggcg ataactatca acaaatcaac gtagaagccg cgctggccga
    11641 tgactcctcg gtgttttaca cctaccaaaa gttaatcgca ctgcgtaagc aagaagccat
    11701 cctgacatgg ggcaattacc aggatctgct gccaaacagc cctgtattgt ggtgctatcg
    11761 ccgtgaatgg aaggggcaaa ccttgctggt cattgccaac cttagccgtg agatccaacc
    11821 ctggcagcca gggcaaatgc gcggcaactg gcagcttgtg atgcataact acgaagaagc
    11881 ctcaccacaa ccctgtgcca tgaatttacg gccttttgag gctgtctggt ggttacagaa
    11941 gtaaatcttc cctaagcccc ggtaatgccg gtcattccgg ggttttgtct gtcagcatgt
    12001 tgtttttgtt gatttttcaa ccagcaaatt cattaaaaaa tttacataac gctgtagcgc
    12061 ccgtcatccg tacgctctgc tttttacttt gagctacatc aaaaaaagtt aaaacatcct
    12121 tgatgcaaag cactatatat agactttaaa atgcgtccca acccaatatg ttgtattaat
    12181 cgactataat tgctactaca gctccccacg aaaaaggtgc ggcgttgtgg ataagcggat
    12241 ggcgattgcg gaaagcaccg gaaaacgaaa cgaaaaaacc ggaaaacgcc tttcccaatt
    12301 tctgtggata acctgttctt aaaaatatgg agcgatcatg acaccgcatg tgatgaaacg
    12361 agacggctgc aaagtgccgt ttaaatcaga gcgcatcaaa gaagcgattc tgcgtgcagc
    12421 taaagcagcg gaagtcgatg atgccgatta ttgcgccact gttgccgcgg ttgtcagcga
    12481 gcagatgcag ggccgcaacc aggtggatat caacgagatc cagaccgcag ttgaaaacca
    12541 gctgatgtcg ggtccataca aacaactggc tcgtgcttac atcgagtacc gtcacgatcg
    12601 cgacattgaa cgtgaaaaac gcggtcgcct gaaccaggag atccgtggtc tggtcgagca
    12661 gaccaacgcc tcgttactca acgaaaacgc caacaaagac agcaaggtga ttccaaccca
    12721 gcgcgacctg ctggccggga tcgtggctaa acactatgca cgccagcacc tgctgccgcg
    12781 tgacgtggtg caggcacatg agcgtggcga tattcactat cacgatctcg attactcgcc
    12841 gttcttcccg atgttcaact gcatgttgat cgacctgaaa ggcatgctga cccagggctt
    12901 taaaatgggg aacgccgaga ttgaaccgcc gaagtcgatc tctacggcaa ccgcggtaac
    12961 tgcgcagatt attgctcagg tcgccagcca tatttatggc ggcaccacca ttaaccgtat
    13021 cgatgaagtg ctggcgccgt ttgttaccgc cagctacaac aaacatcgca agaccgcaga
    13081 agagtggaac atcccggacg ccgaaggcta cgctaactct cgtaccatca aagagtgcta
    13141 cgatgccttc cagtcgctgg agtacgaagt aaacaccctg cacaccgcca acggtcagac
    13201 gccgtttgta acctttggtt ttggtctggg caccagcagg gaatcgcgcc tgattcagga
    13261 atcgatcctg cgtaaccgta tcgcaggcct cggtaaaaac cgtaaaactg cggtgttccc
    13321 gaaactggtg tttgcgattc gcgatggcct gaaccataaa aaaggcgatc cgaactacga
    13381 catcaaacag ctggcgctgg agtgcgcaag caagcgcatg tatccggata tcctgaacta
    13441 cgatcaggta gtgaaagtca ccggttcgtt taaaaccccg atgggctgcc gcagcttcct
    13501 cggcgtgtgg gaaaatgaaa acggcgagca gatccacgat ggtcgtaaca acctcggcgt
    13561 gatcagcctg aacctgccgc gtattgctct ggaagcaaaa ggcgatgaag ccaccttctg
    13621 gaagctgctg gatgaacgtc tggtgctggc acgtaaggca ctgatgaccc gtatcgctcg
    13681 tctcgaaggc gtgaaagcgc gcgtcgcccc gatcctctat atggaaggtg cttgtggcgt
    13741 acgtctgaat gctgacgatg atgtttctga aatcttcaaa aacggtcgtg cgtctatttc
    13801 gctgggttac atcggcatcc acgaaaccat taacgcgctg ttcggcggcg agcatgttta
    13861 cgacaacgag cagcttcgcg cgaaaggtat cgccattgtt gaacgtctgc gtcaggcagt
    13921 ggatcagtgg aaagaagaaa cgggttatgg tttcagtctc tacagcacgc cgagtgaaaa
    13981 cctgtgcgac cgcttctgcc gtctcgatac tgctgagttt ggcgtggtgc cgggcgtgac
    14041 cgataaaggt tactacacca acagtttcca ccttgatgtg gagaagaagg tgaacccgta
    14101 cgacaagatc gactttgaag cgccttaccc gccgctggcg aacggtggtt tcatttgcta
    14161 cggcgagtat ccaaacattc agcacaacct gaaagcgctg gaagatgtct gggattacag
    14221 ctatcagcat gtaccgtatt acggcaccaa tacaccgatt gatgagtgct acgagtgtgg
    14281 ctttaccggt gagttcgagt gcaccagcaa aggcttcact tgcccgaaat gtggtaacca
    14341 tgacgcctcc cgtgtgtcgg taactcgccg cgtgtgcgga tatttaggta gcccggatgc
    14401 gcgtccgttt aacgccggta agcaggaaga agttaagcgc cgcgttaaac atttggggaa
    14461 tgggcagata ggttaatctt cactctgcgt taagcaaatg ccggatgcgg cgtaaacgcc
    14521 ttatccggcc tacatttggc aacggctgta ggcctgataa gacgcgacag cgtcgcatca
    14581 ggcattgatt accggatgca gcctacacgc ggactgataa gccaggcgaa ataatgaatt
    14641 atcatcagta ctatcctgtc gacatcgtca acggccccgg cactcgctgc accctgtttg
    14701 tctccgggtg tgttcatgaa tgccccggtt gctataacaa aagcacatgg cgggtaaatt
    14761 ctggtcagcc atttaccaaa gcaatggaag acaagatcat taaagatctg aatgacactc
    14821 gtatcaaacg ccaggggatt tccctctccg gcggcgatcc gctgcatccg caaaacgtgc
    14881 cggatattct gaaactggta aaacgcatcc gcgccgagtg tccgggtaaa gacatctggg
    14941 tgtggacagg ctataaactc gacgaactca acgctgcgca aatgcaggtt gttgatttga
    15001 ttaacgtgct ggtcgacggc aaatttgtgc aggatttaaa agacccttcc ctgatctggc
    15061 gcggcagcag caatcaggtg gtgcatcatt tgcgatgata agttccctgt tacctgagtg
    15121 caggcaacag ggaaatgagg aattaacgat acttctggtg ataggcgttg cgggtcgttt
    15181 tcagttgctc tgcagcagcc tgcgcttctt ttactttacc ttcatttgcc agcttcagcg
    15241 cgtcgtcaat ctgaccgacc agaatgtcga aaccgtggcg gaaatctttc atttccgggc
    15301 tgtccggtga tttatcttcg agcttcggcg gcgttgcttt ttgcgcatcc agcgctgcgg
    15361 cgcgcatctt cgttaacgcg tctttgactt gcgtcgcgtt atcggctttt tcgaccactt
    15421 ttaaattgtc gttgagggtt tccatattgt cttcaagatc agcggcaaac gacgccgaac
    15481 tgaataccaa cgaggagact gcaagaatag ctaacaggct tttacgcatt gctcacttcc
    15541 ttttttattt acacttcatc cttcacactg cctcatcgac agctacgctc attcgaccgg
    15601 gtcacgtact acgagtacgc tccccggctc tcattcactt tccgtctcga tgcagtgcga
    15661 atgattttgt gtaatttttt agtttatagc gcggcaggtc gcgccagttt ttttactgtc
    15721 cggcgatttt catctccggc aacagcacag aaccacactg tatattactg cgtgtttcaa
    15781 tatcgttacc gacggtgaca atattgcgcc acatatcttt taaattacct gcgatggtga
    15841 tttcgctcac cggatactga atttcgccat tctctaccca gaagcccgca gcaccacggg
    15901 aataatcacc ggtaatggca ctcacgccct ggcccatcag ctccgtcacc accagcccgg
    15961 tgcccatctc tttgagcatt tgttcgaagc ttagcccttg tccggcaatc cgccagttgt
    16021 gaataccgcc cgcatgtccg gtgcttttca gcccaagttt tcgcgccgaa tagctggtca
    16081 gcagccactg agtcaggatg ccatctttaa taatatcgcg acgctcggtg cgcacacctt
    16141 cgctgtcgaa tggcgtcgac gccagccctt tcagcagatg cggatgctct tcaatggtca
    16201 gccagtccgg cagaatttgt ttacccagcg aatccagcag gaaggtagat ttacgataaa
    16261 ccgatccacc cgctatcgcc cccaccagat gcccaaaaag cccagttgcc acttcattgg
    16321 caaaaatgac tggcgctttc atggtggaga gtttacgcgg taacaggcgc gataaagtac
    16381 ggcgagcaca gtcggcccca acccactctg gcgtttgcag atcgctcatc gcgcgcccaa
    16441 tggtgtaggc gtaatcacgc tccatatcgc cattttcttc ggcaattaca cagctggaga
    16501 gcgaatgacg cgttgagcag taaccctgca acatgccgtg gctgttgcca aaaactttga
    16561 caccgtagtg gctgttaaag ctgccacctt cggtattggt gatacgttta tccgcctgta
    16621 atgctgcctg ttctgcgcgg gccgccagtt caatggcttc atccggggaa acttccgcag
    16681 ggtggaacaa gtcgagatct ggtgcgtcaa aggccagcag ctctttgtct gccaggccgg
    16741 cacagggatc tggcgaggta taacgggcaa tatccagcgc cgcctgtaca gtacgggcaa
    16801 tggcctgcgg gcttaaatcg gtggatgatg cgctaccttt gcggttctgg tgataaacag
    16861 tgatccccag cgcgccatcg ctattgaatt caacattctc cacttcacca taacgcgtgc
    16921 ttacgctaat gccggtggtc ttgctgacgg caacttccgc accgtccgat ttgcctgagg
    16981 ccaactccag cgcagtggaa actgcttctt ccagaatctt gcgctgcgct tcaacttgag
    17041 agattacttt cattgcaagt gccataatgt agagagtttc tctgaagtct aacagagaac
    17101 cgtttttcag tgcgcatctt aactggtaac attagcctct ttttttaagg agcctgagat
    17161 gactaagcag cccgaagact ggctcgacga cgttcccggt gatgacatcg aagacgaaga
    17221 cgatgaaatt atctgggtca gtaaaagtga aattaaacgt gatgccgagg agctaaaacg
    17281 ccttggcgcg gaaattgttg atctggggaa aaacgcgctg gataagatcc cgttagatgc
    17341 ggatctgcgc gcggctattg agctcgccca gcgtattaag atggaaggtc gccgccgcca
    17401 gttgcagctc atcggtaaga tgctgcgcca gcgcgacgta gagcctattc gtcaggcgct
    17461 ggataagctg aaaaaccgtc acaaccagca ggtggtgctg ttccataaac tggaaaacct
    17521 gcgcgatcgt ctgatcgatc aaggtgatga cgccatcgct gaagtattga atctgtggcc
    17581 cgatgccgat cgtcagcaac tgcgtacact gatccgcaac gcgaagaaag agaaagaagg
    17641 gaataagccg ccgaaatccg cacgccagat tttccagtat ctgcgcgaac tggcggagaa
    17701 tgaagggtaa ttttcgttgt tgatgtgagt atggtttgcc ggatgcggcg taaacgcctt
    17761 atccggcaat gctatatcag gctgaagccg aattactgcg cagcttccgc cttcttcgcc
    17821 agaccatcca gcagtttctg atggatccca ccaaaaccgc cgttgctcat caccagaata
    17881 tggtcgccag gctgagcggt tttcaccacc atatctgcca gcgtatccac atcgccactc
    17941 cagtgtgcag gctgaacgca ggcttctgcc acttctgcca cctgccacgg aatatgcgcc
    18001 ggttgcagca ggaagacttc atcggcacga cctaatgaag gtgccagatc gtctttgcag
    18061 atccccattt tcatggtatt cgagcgcggt tccagcacag caataatgcg cgccgtacca
    18121 ccaactttgc cacgcagcgc cgccagcgtt gccagaatcg ccgtcgggtg atgggcaaaa
    18181 tcgtcatata ccgtgacgcc attcgcttca ccacgcaact ccagacggcg acgagcatta
    18241 ataaacgaac ccagcgcgtt agcggcatct gccgacgcta caccaacatg gcgagccgct
    18301 gcaatcgcca tcaggccatt gtgcatatta tgttcgccta ccagcgacca tttcacttcg
    18361 cccacttttt cgccatccag caaaacttcc cattcggaag catcggtggt cagctttttc
    18421 gcctgccagt gaccctgctc acccaccagc tcctgctcgc tccagcagcc catcgccatg
    18481 gtctgtttca ggttgatgtc attttccggc cagataatac ggccctgccc cggaacgata
    18541 cgcaccagat ggtggaactg tttctggatc gctttcaggt cgtcaaagat atcggcgtga
    18601 tcgaactcaa ggttgttgag gatcagcgta cgcgggcagt aatggacaaa tttagagcgt
    18661 ttgtcgaaga aggcgcagtc gtactcatcc gcttcgataa caaagaagtc gctttcgccc
    18721 agacgcgccg aaacctcaaa gttccccggc acaccgccga tcacaaatcc cggtttgtaa
    18781 ccacactgtt ccagaatcca ggtcgccatt cccgcggtgg tggttttgcc gtgtgtaccg
    18841 gcaacggcca gcacccagcg gtcgcgcagc acaaaatcgt gcagccactg tggacctgac
    18901 atataaggga tgtttttttc cagtaccgct tccacacacg gatttccacg ggtcatggcg
    18961 ttgccaataa tcaccagatc cggctgcggc tcgagctggc tggcatcgta accctgaatc
    19021 agctcaatgc cttgcttctc aagtaaggtg ctcatcggcg gatacacatt ggcgtccgaa
    19081 cccgttactt catggcctaa ctggcgcgcc agcatcgcca gaccgcccat aaatgtgcca
    19141 caaattccta aaatatgaat gcgcatacgt cactatcctt ttttaatctg ccgctcattt
    19201 tacgcatatg tcgggcaagt gagaaacgca tttcaggata atccgcaatt tgctggcgcg
    19261 attcacctga gcgcaacatt gagattattt gttaagattg ttgcggtcgc tttactccat
    19321 aaacattgca gggaaagttt tatgaaaacg ttaggtgaat ttattgtcga aaagcagcac
    19381 gagttttctc atgctaccgg tgagctcact gctttgctgt cggcaataaa actgggcgcc
    19441 aagattatcc atcgcgatat caacaaagca ggactggttg atatcctggg tgccagcggt
    19501 gctgagaacg cgcagggcga ggttcagcaa aaactcgact tgttcgctaa tgaaaaactg
    19561 aaagccgcac tgaaagcacg cgatatcgtt gcggggattg cctctgaaga agaagatgag
    19621 attgtcgtct ttgaaggctg tgaatacgca aaatacgtgg tgctgatgga ccccctggat
    19681 ggctcgtcca acatcgatgt taacgtctct gtcggtacca ttttctccat ctaccgccgc
    19741 gttacgcctg ttggcacgcc ggtaacggaa gaagatttcc tccagcctgg taacaaacag
    19801 gttgcggcag gttacgtggt atacggctcc tctaccatgc tggtttacac caccggatgc
    19861 ggtgttcacg cctttactta cgatccttcg ctgggcgttt tctgcctgtg ccaggaacgg
    19921 atgcgcttcc cggagaaagg caaaacctac tccatcaacg aaggaaacta cattaagttt
    19981 ccgaacgggg tgaagaagta cattaaattc tgccaggaag aagataaatc caccaatcgc
    20041 ccgtatacct cgcgttatat cggttcactg gttgcggatt tccaccgtaa cctgctgaaa
    20101 ggcggtattt atctctaccc aagcaccgcc agccacccgg acggcaaact gcgtttgctt
    20161 tatgagtgca acccgatggc attcctggcg gaacaagcgg gcggtaaagc gagcgatggc
    20221 aaagagcgta ttctggatat catcccggaa accctgcacc agcgccgttc gttctttgtc
    20281 ggcaacgacc atatggttga agatgtcgaa cgctttatcc gtgagttccc ggacgcgtaa
    20341 tctcctgcgc ctctccacac ggagaggcgt ttttattgct gcgcaatgtt gactcttgtc
    20401 actggcgaac tctgacgatt ctcccacagc accgttaatc cacgctgtaa tgctataaaa
    20461 ataaacaaca aaataccgat ggcgattttc gtccaccagg aactcagcgt gccatcaaag
    20521 tttatgtaag tctgaatcag tccctgaatc gccacgccaa aaagcgtccc taataccgtt
    20581 ccaacgccac cgctcaaaag cgtaccgcca attaccactg aggcgatagc atccagctcc
    20641 acacctacgc ccgccagcgc atatccggcc tgggtataaa tcgagaagac aatccccgcc
    20701 agcgttgcca gtccggtgga gagcatataa atgcgaatag tggtgctgcg agtggaaatc
    20761 cccatcagat tcgccgacgt tgcgttgccg ccaatggcgt atacctgatt accaaaacgg
    20821 gtacgatgcg cgaggaatat gccgataacc accaccgcca acatcagcag tcccatcgca
    20881 cttaagcgac cgccgccagg gattttccac gcaaggcttg agagcgtgtc ataaatggga
    20941 tggtttatcg gaatcgactc ttccgaaacg agatagctga cgccgcgcaa aaagaacatc
    21001 cccgcaagcg taatgatgaa cgccgggatc ttcagggcgt cgatcagaag ccccataaat
    21061 gcgccgaagg cacagcccat caccagcacc agcggaaacg ccagcagcgg cgagaggccg
    21121 aaatcgccaa tcacttttgc cagaaacacg ccggtaaagg cgatcaccga accgacggag
    21181 agatcgatcc caccggagag gatcacaaag gtcatgccaa cggcaatgat cccaagaaag
    21241 gcgttatcgg tcaggatatt gcagatcact cttgtggaag caaaaccagg aaactgcgtc
    21301 aggcagtaga gatagcccag cacaaagacg ccgatggtga tcataagcgg caagttacgt
    21361 tttatcacgg ctacgcactc ctttaatcag actgataaag cgttgcgact gaacaatcag
    21421 cacacaaagc accaccaccg ctttcaccac ctggttcatc tctggcggaa agcccgaaag
    21481 caaaattccg gtattcattc cctgaataat cagcgcccca actcccgaaa gcagcaggtt
    21541 aaagcgaccg cccatcagcg atccaccgcc aatcaccacc gcgagaatgg catccagctc
    21601 cagccataat ccggcgttgt tggcatcggc accgcgaata tccgccgcca cgataatgcc
    21661 cgcaatcgcc gcacatagcc cgctcaacac gtaggtgagc atgacgatga ttcgtgtgtt
    21721 gactccggca ttttttgccg cccgaatgtt gataccaacg gcttcgataa acatccccag
    21781 cgccgttttg cgggtcaaca gccagaacag gagaagcgtc agcaccgcga taatgaccgg
    21841 cgttggcagg aacaacagcg atccgctgcc aaaccatgag agatccggcg agttaaacgt
    21901 gacgatctgc ccggcggtga tcagttgcgc cacgccgcgc ccggcgacca tcaggatcag
    21961 ggtggcgaca aacggctgaa ttttgaggat cgctaccagt atgccgttcc acaatcccgc
    22021 taggatgccg gtgcccaggg cgcttaacaa aacaatcggc aggctgaatc ccgcgaccgt
    22081 catcgcagcc gttgtggctc cggcgatagc catcaccgcc cccacagaga gatcgatacc
    22141 accggtggcg atcaccagcg tcataccaat cgccagtaac gcaacgggag ctgcacggtt
    22201 aagaatgtcg atagggctac cgaacaaacg cccatcctga agcaccacct gccagaaatg
    22261 cggggctacc aggctatcga ccagcagcac cagcaacagt gccgccagct gcggcatccc
    22321 cgtcggccag tgaaaacgcc tttttggcgg cgtagtgtca gggagagatt gaggcatcac
    22381 actgttctcc ttacgccgca atggcgttca tgatcgccgg aacggaaagc tgtgccagcg
    22441 ggatctccgc cacctgtttg cgatcgcgca tgatgatcac ccggtcggca tagcccacca
    22501 gctcttccag ttcggaggag atcaccagca gcgccagacc gtcagcacac agcgtttcaa
    22561 tcaggcggat gatctcggcg tgggcaccaa catcaatgcc acgggttggc tcatcaagga
    22621 tcagaaattg cggtcgggtc agtagccaac gtgaaagcag cactttttgc tgattgccac
    22681 cggagagaaa ttcaatcggt tgttcagttg aaggtgtgcg aatgccaagc tggcggataa
    22741 agcgttcggc aatctcttgc tgctctttgc gggaaatggg tcgtagccag ccgcgctggg
    22801 cctggagagc gaggatgata ttttcccgca ccgaggcggc ggcgatgatg ccatcggttt
    22861 tcctgtcttc cgggcagaat ccgatgccca gtactgaagc ctgatgtggc gatcgcaggg
    22921 tttgcggttt gcctttgatc aacgccgtgc cgctgtcagc aggtttgata ccgaagatca
    22981 cttcggcggt ttcggtacgt ccggatccca gcaagccagc cagaccgacg atctcgccgg
    23041 ggcgcacttc gagatcaaac ggtgctatcg ttcctttttt gccgtaattt ttgaacgcgg
    23101 caacgggttt gtcgctcaac aatgttcgcc cggcacgctg tagcacgtgg gtatccagct
    23161 cgcgccccag catcattttt accagttcga tctgcggtag ctcgcgcgtt tcccgacagc
    23221 ctacgaaact gccgttgcgt aagacagtga tccgatcgct gacctgatag acctgatcga
    23281 gaaagtgagt gacaaagatc aggctgacgc cgcgatcgcg caactgacgc atcaggccaa
    23341 ataatagctc cacttcctgg gtgtcgaggc tggcggtggg ttcatcgagg atcagcactt
    23401 tggcggagag atcgatagcc cggcaaatgg cgacgatttg ctgcatcgcg actgaaaagc
    23461 ggttaagcgg ttcgcgcacg tcgaggaaga aaccgtaaga tgccatcagt tcggtggcgc
    23521 gcttttccat ctctttacgg cgtagaaggc cgaaacgttt gggttcgcga cctatgaata
    23581 gattatcagc aaccgacata ttgggtagca ggttgacttc ctgatagacg gtgccaatac
    23641 cgagttgttg tgcatgggcg gtatttttcg gtgagatagc ctggccttcc agccagatgg
    23701 tgccgcgatc ggcgtggtat acaccagtta atgctttgat tagcgttgat tttcccgccc
    23761 cgttttcacc aagcagcgcc atgatttcgc cacggcgcag gctgaaatca acgttgtcta
    23821 acgctttgac gccggggaaa aatttactta atccttcggt gcggaggatc tcctggtgtt
    23881 ggtcggtcgt catggtttcc cccggtgtaa agcgtgctcc cagtaggcat gataagacgc
    23941 ggtaagcgtc gcatcaggct tcagtgcacc atcgccggat gcggcgcgag cgccttatcc
    24001 ggcctacgaa tcggcatcag gcatagcaac cctcaatacc ccatattttt cttcttctct
    24061 aactcttctt ttgcggtatc aggcaggtaa agggtggatt tggttaacgt cagcttttca
    24121 ggcatggtgc cgtctttttt gtatttctcc agcgcatcga aagcgggtcc cgccatgttc
    24181 ggcgtcagtt caacactggc gttcgcttcg ccatccatca tcgctttgta gatgtccggt
    24241 acaccgtcaa tggaacccgt gaggatatct ttgcccggtt tcaggcccgc ttctttaatt
    24301 gcctgaattg caccaatcac catgtcgtcg ttatgggcgt aaaccatgca gatgtttttg
    24361 ccgttgtttt ccgctttgat aaagctctcc atgacttctt tgcctttact gcgggtgaag
    24421 tcacctgact gcgagcggat aattttgata tttggcgcat tcttaatggc ttcggcaaag
    24481 cctttcttac ggtcaatggc gacgctggcc ccaacggtgc cctgcagctc caccacgttg
    24541 catggtttgc cattcacttc ttttaccagc cagtcaccga tcaacttacc ttcgaggatg
    24601 ttgtcggcag taacggtggt catatagaga gatttgtctt tcacatcgat ggaacggtcg
    24661 agcaagaaga ccgggatttc ggcatctttc gcctctttta ataccggttc ccaacccgtc
    24721 gcaaccactg gagcgataaa gatcgcatcc accccttgtg caacgaagga acgtaccgct
    24781 ttaatctggt tttcctgctt ttgctgacca tcggcaattt tcaacgtaat tccgcgtttt
    24841 tcggcctcac ttttcgccac attggtttct gcggcgcgcc agccggattc cgatccgacc
    24901 tgcgaaaatc ctccggttaa tggagctgcc aacgccatag acgacatggc tgccgagact
    24961 gcagagacta caagtaagcg tttccacata gggttgtcct cgtcggggta attattattg
    25021 gtgaaaagat gttcgcgaaa aaactataga caattcgtta tgtaacggat tgcgttacat
    25081 cacacttcaa aaaagtaaat aaaacgttaa tcacaagttt gtaatcgctt tcatctcact
    25141 atgaaaaatg cggctacggt tatggatttt cctgctctgt ataccgtctt aaaactggcg
    25201 aaaaaggaaa atgaagacga aaacaagcaa agacattcgg cgcgagttgg ctataatact
    25261 cggcacttgt ttgccacata tttttaaagg aaacagacat gagcttactc aacgtccctg
    25321 cgggtaaaga tctgccggaa gacatctacg ttgttattga gatcccggct aacgcagatc
    25381 cgatcaaata cgaaatcgac aaagagagcg gcgcactgtt cgttgaccgc ttcatgtcca
    25441 ccgcgatgtt ctatccgtgc aactacggtt acatcaacca caccctgtct ctggacggtg
    25501 acccggttga cgtactggtc ccgactccgt acccgctgca gcctggttct gtgatccgtt
    25561 gccgtccggt tggcgttctg aaaatgaccg acgaagccgg tgaagatgcg aaactggttg
    25621 cggttccgca cagcaagctg agcaaagaat acgatcacat taaagacgta aacgatctgc
    25681 ctgaactgct gaaagcgcaa atcgctcact tcttcgagca ctacaaagac ctcgaaaaag
    25741 gcaagtgggt gaaagttgaa ggttgggaaa acgcagaagc cgctaaagct gaaatcgttg
    25801 cttccttcga gcgcgcaaag aataaataag ttcttctagc gcaataaccc tgaacgccgg
    25861 gcttcggtta gtaagggttt tttatgcccg cgataaataa actctctatt ccaccatcat
    25921 tattctcagc ggttgcaagg cttgaacggt aagaacaagc aaacccgacc accattttgc
    25981 tgttcatagc cacttgctgg aagttagccg acctcactca tactcaccgt tcaaagcgtg
    26041 cccccgtttt acccctgcac atttgtgaac gccagcagag ttttttatgc gattgcagat
    26101 atggaaagcg gctcggagag aaaagcaaaa gatggaaaag ttacaaaaaa aaatcgcgag
    26161 atacatcgca taaaacaacg ccacccgaag gtggcgtgct tttattatta cttatccctg
    26221 tctaaccagt cgccgctttc aattagcttt aatccatcga cgggtcgttg ataaacgtac
    26281 atccatgcac tcccgtacgg cgtctgaatc aactggcgcg cgtattcacc gcccctggtg
    26341 cgcaaggcat caagttcggc cagcgtggcg ttgtcaatac gataaacttc accgtgtacc
    26401 gttccgttcc ccggaactgc gcctggatag tggcccaggc tatacaactg gtagttatcg
    26461 atactgaaat cgcccagtaa ctgggcattg gtcatccagt gactgttgcc ttgtttgtgg
    26521 cgtaaactgc cgtagacaaa tattcgcatt gctaaaactc gaactgatag agcaaatcca
    26581 gtgcctggtc tacaccagac acggcttcca gatatagctt aggcatcagg cgataacgta
    26641 acgtgagtgt tgctatagag tcaaatatac ccacgccgta tttcacttgc agacctggca
    26701 atacatagcc gctgaccact acctgggagg agtcgcctac tccctgggtg tcgagcgcta
    26761 aattgcttac gccaaacgtc tcgccgattt tacccacaat ctggccactt tgcgcaaccc
    26821 ccagaccaat cagcatcgag gtcattgccg cactgtcgct ctgatcgctc tccagccctt
    26881 gtccacgtag caaataagac aatgcagctt gttgcgacat cgccgggtca gagaagatct
    26941 ccgctttcgg ttcgtccgcc agaccagtga cgcgaactcc ggcaattacg tcgtcttctg
    27001 tagcatccgg gttacgaata gcttcaatat taagatacgg ttgatccggc ggaccagaga
    27061 acagtaactc acccttacgc acaatcagat cctgaccata ggcatggaag cgcccttcag
    27121 ggatgttgat ctgcccgttc aggcccagcc cttgtttgtc ctgtacgacg ttaagatcgc
    27181 ccgtcagccg cgctttcagg ccaaaggcgt caatgcgcac attgttgcca acgtggacaa
    27241 tcaggttact gttaatcgga atcgacgccg ttttcggctc ttccggttgc aggttatcgt
    27301 taagcatcac cacatcgctg gagacgccta ctgcgctttc cggcagatcg tgcaccacga
    27361 tgcgcgccca cgggacatcc acgcgaccat cgagggtaaa caggtttggt gtagcctcga
    27421 atacaacatc tggcgataca tccattcgta ccatcggcgg cacggtgatc cgcactttac
    27481 tgcctttcgc cgttactcgt gcccgccagt tttcaatttg gctccagtcg gcatcaccgt
    27541 tcaggtagat ttcaccctgc tgggtccgta ctgtaccggc aagcgtcgag cgcataccgt
    27601 taaagttgac cgcaagctgg ctcggctgca tatcaaacgg cataaagttg ccgtcgatat
    27661 ccacacccgt aacctgaagc tgaccaaaca actgcgggct ttgcacatca ccacccagac
    27721 gcaagttggc actcaccatc cccgctgctt tttccccacg ggtaaagatg gggtttatca
    27781 tcgcaaggtt gaagttacgg atattgacgt tgccaccaag attacggcgg ccttgcggat
    27841 cggtcacctg cacctgtcca tcaaactggc cgttattggt caggcggatg gtccagccca
    27901 attcggcacg gttgttacgc aattccgccg tcagattcag tgtctgaaac gccaccggca
    27961 gcgccgcatc gttgacggtt tgcgttacct gcacgttacg ccccgaaagg gtgatactgc
    28021 cctgcggcag cccctctttc gtggtgtccc aggcaacatc cgctttaccc gtgaagatac
    28081 cgctggcctg agtggtttct ggcataaacg gtttcagcat ggcgaggtcg aagcggttga
    28141 gattcaccac cgcacgccct tcggcccccg catcgatagt ttgcggcacg cacagttccg
    28201 cattcgggtt aagccagcag tgtggcccga tgctgatttt ttgctcctta ttgcggtaat
    28261 ccagcgcaat atcgcgggtc agcgaccacg ggccgaccgg cgtctggaaa cgggtattgc
    28321 taagagttcc cttccagcgt tcttctttgc gatcaaaact tcctgccaga ttaagctgcc
    28381 cggagacagg ctcgccctga atccgcaact gtagctcgtg ctgcttttcg ctgcctttgg
    28441 cattcagggt gacgaggttg atatttacat ccggctgcga aatttgctca acgcgtacgt
    28501 cgagtttccc ggcgatctga tcggtggatt taatgtcgcc ttccacgcga acctgcgcca
    28561 cggaaagttc ctgccagcgc aggccgcgcg cggtgatatc tgccagtagt tgtggcgctt
    28621 ccaccgtgcc gcgtactttc accagccctt tcgctgtacc gccaagcccc ggcagcgcgt
    28681 tatccagccc cggcgcgttg atggtggcat caagattgag atcttttacc cccagctcgc
    28741 ctttcacttc ggcactgttt ggcccgagtt ccagatgaag cccagggatc atccactgca
    28801 tataactgtt gcctttcagc gtgccgtcaa cgttcacttt gttctgtttg acgttaccgg
    28861 tcagcttcag ttctggcacc tccatctgcc aggtgccgcc gtacaggcta ccgcgggttt
    28921 taatcaagcc attgagtttc gacggccact ccgggaactc tttggcggtg ttaatgccat
    28981 taagcgttag ctcaccgcgc caactaatgg cctgctgcca gtcgagcaac gccttgagtt
    29041 cagttttccc ttccagcgcc gcgacggtga gtttgtcgag attgacctgc tgttcattac
    29101 ctttggcatc aagggtaatg gtggctggcg ggatctcctg tcccttcact gccgtacgca
    29161 tagagagcgt gtaatcggtc attttgccgg taagtttcag tttcagatca tccgcctgat
    29221 actgcttctc accagtgaac ggccagtaaa gctgtttgct gttcacttcc acgttgagtg
    29281 gcaatccggc ttccgccagt cgcgtctggg cgcgtaaatc catatccacc ggaccggaaa
    29341 ggttaacgcc tatctccagc tgttcgcgca gcgcgccgcc cactttcagc ttcacctttt
    29401 cacctttcaa cggctccacg ttcagtgtgc tgttgaggat gatatccacc ggccagttgt
    29461 ctgacagctg cgccgtgccg ctggcgttga cgatcccctg gttggaatcg atatccaggg
    29521 cgtccagttt agtattgccg tcaatgctgc tcactttcag cagcatggtg cgcacggtga
    29581 tgtccgtgtc gcccgtcacg cgcagctgct cgcccttaaa ctcttcaatg ttcaggttaa
    29641 gcggcaaatg cacgtcggtc atttccggca ataccgggcg agaaaagaga tctttcagcg
    29701 tttcgccgag cggcttttca tccggctgcg gattttcaat cttcggttcg acgacctctt
    29761 cctgcgccac ttccgccact ttcggcagag caatcagcag gcctttcagc gacgtcggtt
    29821 tcagggtcag ggttttctcc tgccagttca ggccggaggt gaagtccatc accgacaccg
    29881 tggtgtcatc aatcttgatg ttgacgttat ccagcgccac ccgtgtcagg gtgatgggat
    29941 acggcgtgga gagatccagc ggaccgctat cttcctcttc ttcaacctgt tcagaaggag
    30001 gcattttttt actgtcgatg ttgacctgaa tgtctttcag cgccaggtca ttaatacaaa
    30061 cgctactgtt ccacaggcac tcaagaccga ccgccagatg cagattaccc gcttttaccg
    30121 ccacgcctgg ctgctcataa cgaacgtcag acaaggtgag atcgcgccag ccgccggtga
    30181 cgttgccaat atccagtcct ggcacccagc gatccgccgc tttaaataca agatgcaggc
    30241 cgctggtggt gcccaccaga aacgccaccg atcccagcaa cagtaagata acgataacca
    30301 cgccgaggct gatttttttc cataaactca taattctggc cccagaccga tgtaaaactg
    30361 taacccgtgt tcgtctttat ccgcgaccgg tacggcaaaa tcgagtttga ttggcccgac
    30421 cggcgattcc cagcgtacgc cgcccccggt accggtttta aagtcgctgc ggcgaatatc
    30481 gcttaccgct tcgccactat cgacaaacac cgcgccccac cattttccgg tcacgttgta
    30541 ctggtattcc agcgatccgg ttatcagctt cgaggcccct ttcaggtcac cgttggcgta
    30601 tttcggggcg atagatttat atttgtagcc gcgaatacta cgatcgcctc cggcgaagaa
    30661 acgcagatcc ggcggtactt tgtcgaaatc accggtttca atccagccca gcgtgccgcg
    30721 tgtcacaaaa cgatggcgat cgtacagtgt gcggatccag acgttctgcg cctggaaaac
    30781 ggagaaatca acatctgaac cccaggccgt gttggagtag tcgatagagt agcgttgcga
    30841 gtcgccccag gttggcatca ggccaccacg agaacgcgtg cggctaatca tcaccccagg
    30901 ataaaacagc atcgtggtgt tggtaatttc accctgggta aagtggtcga ggctccagcg
    30961 caggttaatg gcacgctgcc agtcgctgga gagatcccag tagcgagaag ccaccagcgt
    31021 agtggagtca gattcggtat cgttcaggtc agtgcgctta aaaccgccct gcaccaaata
    31081 atattgttcc agtggattct tcagcagcgg cattttatag ctgaagtcga ggatctgttc
    31141 cggcgcggaa atactggtac tggtggtcag actgtgacca taagagttca tccacggctt
    31201 tttccacgtc gctttcacgc gcggtcccac gtccgtagag taaccgaccc cggtttcgat
    31261 ggtgttttct gttcgcggcg aaaccacgcc cgtcaatggt aatactttcg tttcgcgcgc
    31321 tttatcaaat tgcggtgcca ctaccaccga attaaaccag ccggtagcag aaagtcggcg
    31381 gttcagttcc gccagatctt tcgattcata ctcatcgccc tctttaaacg gcaccagatt
    31441 ttgcaggtat tcatcgcgga tttgtgatcc ttcaaaggtc acatgcccaa agcggtaacg
    31501 ttcgccactg ttataatcaa tatcccagaa ggctttatgc aggccgagcg caatgcccag
    31561 ctgcgctttg gtaaattcgc tatcgaaata acctttacgc aacgcaatac tggttaagga
    31621 ctttttgaaa ttttcataat cgccctggtt cagtaccgta ccaatagccg ggcgagtatc
    31681 gagcaatttc aaatagtctt tatcggtccg cgcgccgccg cgcaatacca catcggtgcc
    31741 gccaattaac accggtacac ctggcgtgac tttggcgatc aatacctgcc gccctttctt
    31801 tggcggtgga cggagatcaa attcaatggt cggctggtaa taacccagcg ctttcagacc
    31861 ttcacggatg gcatcatcga cgcgtgcgcg aaagcgacgg tctggcgtca cttcatcact
    31921 ttcaatcgta gaaagctgcg cacgaacgtt cttttccagc tgtcccgata acccctcgac
    31981 ctgtagacgg acgttcgcgg cgacggcaga tccgcttaag cagagtaagc ttacacagca
    32041 taactgtcgg atatagcgca cattttctcc tgaatatcct tttttcctac ccctggaacg
    32101 ccgttaaacg gcttaacaaa aatccagtaa tatggattaa aaagcagact aaaccccaaa
    32161 tatttcttat gttttacttt agacctattc acggtggtta ttgtgtgcaa atacgcctct
    32221 tgttacaacc ttaaccccaa tgaccgattt tcgggagagc gacaccatga gtttatttga
    32281 taaaaagcat ctggtttccc ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt
    32341 agccacgctg catgcggtca acggtcactc aatgaccaat gtacctgacg gaatggagat
    32401 tgccattttt gcgatgggtt gtttctgggg tgtggagcgt ctgttctggc agttacccgg
    32461 cgtttacagc accgccgcag gctataccgg cggctatacg ccaaatccga cttatcggga
    32521 agtgtgctcc ggtgatacgg gtcatgccga agcggtacgc attgtttacg atccttccgt
    32581 catcagctat gagcagttgc tacaggtatt ttgggagaat cacgatcccg cccagggaat
    32641 gcgtcagggc aatgaccacg gcacgcagta tcgttcagcg atttatccgc tgacccctga
    32701 acaggatgcc gcagctcgcg ccagtctgga acgttttcag gcggcgatgc ttgccgccga
    32761 tgacgatcgt cacatcacca cggaaatcgc taacgccaca ccgttttatt atgccgaaga
    32821 tgaccaccag caatatctgc ataaaaaccc atatggttac tgtggaattg gcggaattgg
    32881 cgtctgtctg ccgccggaag catagcgtta cgggtacaaa tgtagtttgt tgataaagtg
    32941 ctctttgtct atgcctgatg cggcttaaac gacttaccag gcctacaaaa tcgtccaaat
    33001 tcaatatatt gcaaggactg cgtaggcctg atcggcatag cgcatcaggc agtattgcat
    33061 ttatcagcag tctgaatttt aaccctctgg cgactttaca gtaccttacg ctatactagc
    33121 cactgaaaat gtcggttcac tttcttcgaa tcggctttca atgtgtattt cacacaaatt
    33181 aatcaacttt cccttccgag gatctggcct gaaaggtcgg ataagatatg ttaaacagta
    33241 ttttagtcat actctgcttg atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcgc
    33301 ttgccgcctc acgcaaaatc aaacttaaac tgctggctga tgaaggcaat ataaatgccc
    33361 aacgcgttct gaatatgcag gaaaatcccg gcatgttctt taccgtggtc caaatcggtc
    33421 tgaacgcagt ggcgattctc ggcggtatcg tcggtgatgc ggcattttct ccagcttttc
    33481 acagcctgtt ctcccgctat atgtcggcag agctctctga gcaactgagc tttattctct
    33541 ctttctcgtt agtgactggc atgtttatcc tgtttgcgga tttaaccccg aaacgcatcg
    33601 gtatgattgc gccagaagcg gtggctttgc gtatcatcaa cccgatgcgc ttctgcctgt
    33661 acgtttgcac cccactggtg tggttcttca acggcctggc gaacatgatc ttccgtattt
    33721 tcaaactgcc aatggtacgt aaagatgaca tcacttctga tgacatctac gcggtagtgg
    33781 aagccggtgc gctggcgggc gtgttacgta aacaggaaca cgagctgatt gaaaacgtct
    33841 ttgagctgga atcccgtacc gttccgtctt caatgacacc gcgtgaaaac gtgatttggt
    33901 ttgatctcca cgaagatgag caaagcctga agaataaggt ggcggaacat ccgcactcta
    33961 agttcctcgt ctgtaatgaa gatattgacc acatcatcgg ttatgtcgat tctaaagacc
    34021 tgctgaaccg cgtgctggct aaccaaagcc tggcactgaa cagcggcgta caaattcgca
    34081 acacgctgat tgtgccggat acgttaaccc tttcagaggc gttggaaagt tttaaaaccg
    34141 caggtgaaga cttcgcggtg atcatgaacg agtacgcgct ggtggtgggg atcatcaccc
    34201 tcaacgacgt gatgaccacg ctgatgggcg atctggtcgg tcaggggctg gaagagcaga
    34261 ttgtcgcccg tgatgagaac tcatggctga ttgacggcgg cactccgatt gacgacgtca
    34321 tgcgcgtgct ggatattgac gaattcccgc agtcgggcaa ctacgaaacc atcggcggct
    34381 ttatgatgtt tatgctgcgt aagatcccga aacgcaccga ttcggtgaaa ttcgccggct
    34441 acaaatttga agtggtggat atcgataact accgcatcga ccagctgctg gtgacccgga
    34501 tcgacagcaa ggccaccgcc ctttcgccaa aactgcctga cgctaaagat aaagaagaaa
    34561 gcgtcgcgta accccccaga aacatcaacg gctcctgaat caggagccgt cttattactg
    34621 catagcactt tttactgcat agcactttgg ttaagccatc tctgtttgca gacgcataac
    34681 ctgacggtta acttcggaca tcactgacaa atgcagctta tccttcactt ttgggataag
    34741 aatcttaccc ttatcaaatt caaaagcgcc aacgtccttg atgtataacc gtccacggaa
    34801 caggatcttt acgtacttcg ccacctgaag tgggttgtac cgttggaaaa ttttcattgt
    34861 tatctcctgc tgagtattac gcctgtgcgg tgccacaatc ggcccaacta ttatgaggcg
    34921 caaattttaa tgcttagtga ctatagacta tccgggcaat gtttccaccg cgtataactt
    34981 tttttaccta aaggttacaa ttattcagaa ttatcttttt accgaagcgc ggtcttcagt
    35041 ataagcattc atttttcata tgatttgtgc gcttgaccgc aaactggcat cacacttgcg
    35101 ggaaattcga taaatagcac atatgattaa aactcagacc caagtggtcg gatcacctgc
    35161 atatcataag aaggaaacac catgacccta cgcaagattc tggcactcac ctgcctgctg
    35221 ttgccgatga tggcttccgc acatcagttc gaaaccggtc agcgagtgcc gccgattggc
    35281 attaccgatc ggggcgagtt ggtgcttgat aaagatcagt ttagctacaa aacctggaac
    35341 agcgcgcagt tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg cacctctgca
    35401 aaagagaaaa acgcgacgct gattgaagcg attaaatcag cgaagttacc gcacgatcgt
    35461 taccagacca ccactattgt taacaccgac gacgcgattc cgggttcagg gatgtttgtg
    35521 cgcagcagtc tggagagtaa taaaaagctt tatccctggt cgcagtttat tgtcgatagc
    35581 aatggcgtcg cgcgcggtgc ctggcagctg gatgaagaaa gttccgctgt agtggtgctg
    35641 gataaggatg gtcgcgtgca atgggccaaa gacggggcgc tcactcagga agaggtgcag
    35701 caagtgatgg acctgctgca aaagttgctc aaataatggc aacaacatag attctgcaaa
    35761 aataattttc ccctatcgtt atctatggga taggggaatt aagtctctga gttcttacgc
    35821 cgaactcata ctggtaacgc tagttgctct tttttaaacg cctcaatggt cgacttatat
    35881 ttcttgcaaa tcttttcttt ggtaatatta ctatctgcgg ccagattcgc acttactatc
    35941 gcatccactt gttcggctaa ttccacttgt tggcaacctg cattaaccag aaaattataa
    36001 ataagggagt aatcttcatc ctccaaaaca acagaggaac cattacgaaa acgttcatcc
    36061 cctccataga ttttaagcgc aaagcccaaa gattctatga ctatggcaaa attatcatca
    36121 attatttttt cgtgaatata ccatccatcg cattctttaa ttccacctcc tggtatttgg
    36181 agttcaaatt taccccgttt taatttgctt actatatcct ccaataaggc ataacctaaa
    36241 gacacgtcac caatcgcctg gtgaatataa cctttaatcc ttttcggtat gtcagactgt
    36301 tttttctgca gaatatcaca ggcatacaat cttctgtttt gttctaacgg aaactcgcgg
    36361 tttttgaaat ccatgaatga gttatactca tttattgtta tgggaatttc ttcgtattca
    36421 aatccactac ctcgcaatgt cattgtatat tcggcaccat tatctttaag gctgaaactg
    36481 ataaggaact tatccatggc gttaacagtt ccattttttg atgaaagtac ctgataacta
    36541 tttttatcaa tctgagttgc gcaacgaaag aactccctca aagttaacag gctatcataa
    36601 ttcgaaatcc aatctttttt cttaaatgca tgctggatat tgttaaaaaa attgtttgaa
    36661 tgtgtttttg tatacgatga ctttacaaat aactccaccg caccaataaa attattgtgt
    36721 ctgatcctgg gtaacatatg acaaccttat tttatttggt tattttatgt cgccattaaa
    36781 tatctcatta gagaagtaaa ctatcaaaaa acgctcatta ttaaaatgta acttattagc
    36841 aattgaattt ttgcacttta aatacaatct ctttttattt ataacttttt aggttgggag
    36901 aaatcaggat ggcagaatca ggaaatacac tgtttctgcc atctgaattt agtaaataga
    36961 cactctgaac cccggattca ggaacgactc gcgcggagtg taatccagcg gtttaccctg
    37021 ccagtcgtga acgtgcgctc cggcagctgc agccacagca tgtccagcgg cggtgtccca
    37081 aatattcgtt ggtccgaagc gcgggtacag ctgcgcctgt ccttccgcca ccaggcagaa
    37141 tttcagcgaa gagccgatgg acgtggtctg atgttcgcca agctgttgca gatactcttt
    37201 cagctccgca tctgcatggg aacggctgat aaccaccagc ggcgggcgcg catcgcggac
    37261 ctgaatctgc ttgcgcacac cgcactcttc tttccaggcc ttgccttctg ccgcgctgta
    37321 cattacgttc attaccggcg catacaccac acctaaaatc ggtttgccat ggtcaatgag
    37381 cgcaatgtta acggtgaatt cgccattacg tttaataaac tctttagtac catccagcgg
    37441 gtctaccagc cagtaacgct gccagtgctg acggacttcc caaccgggag gatcttcttc
    37501 agaaaggacc ggaatatccg gtgtcagcgt acgtaaaccg tccatgataa cggtgtgtgc
    37561 ggcaatatcc gctgccgtta ccggagaatt gtccgctttg ctgacgacgt ccatcggttt
    37621 cgtcccgtcg tagacctgca taatggcatc gcctgcattc cgtgcaagct ggcatacttg
    37681 atctaacatt tctccacctc gtctctgtga gcggtgttaa cttattgttt tacttatacc
    37741 ctatcgttaa tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg taaaattgtg
    37801 gcattcttca ctgttctata agtaagacgt ttattctccc ttttctttcg tattcccgat
    37861 gataaaagga tgtccctgat gattaagttt agcgcaacgc tcctggccac gctgattgcc
    37921 gccagtgtga atgcagcgac ggtcgatctg cgtatcatgg aaaccactga tctgcatagc
    37981 aacatgatgg atttcgatta ttacaaagac accgccacgg aaaaattcgg actggtacgt
    38041 acggcaagcc tgattaacga tgcccgcaat gaagtgaaaa acagcgtact ggtcgataac
    38101 ggcgatttga ttcaggggag tccgctggca gattacatgt cggcgaaagg attaaaaaca
    38161 ggtgatgttc atccggttta taaggcgctg aatacgctgg attatacggt cggtacactc
    38221 ggcaatcatg aatttaacta cggtctggat tacctgaaaa atgcgctggc gggagcgaaa
    38281 ttcccttatg taaatgccaa cgtcattgac gccagaacca aacagccaat gtttacaccg
    38341 tatttaatta aagataccga agtggtcgat aaagacggaa aaaaacagac gctgaagatt
    38401 ggctatattg gcgtcgtgcc gccgcaaatc atgggctggg ataaagctaa tttatccggg
    38461 aaagtgacgg tgaatgatat taccgaaacc gtgcgcaaat acgtgcctga aatgcgcgag
    38521 aaaggtgccg atgtcgttgt cgttctggca cattccgggc tgtctgccga tccgtataaa
    38581 gtgatggcgg aaaactcagt ttattacctc agtgaaattc cgggcgttaa cgccattatg
    38641 tttggtcatg ctcacgccgt tttcccgggt aaagattttg ctgatatcga aggggctgat
    38701 atcaccaaag gcacgctgaa tggtgttccg gcggtaatgc cgggcatgtg gggcgatcat
    38761 cttggggtgg tcgacttaca actcagtaat gacagcggta aatggcaggt gacgcaggcg
    38821 aaagcggaag ctcggccgat ttacgacatc gccaataaaa aatccctcgc ggcggaagac
    38881 agcaagctgg tagaaacact caaagccgat cacgatgcca cacgccagtt cgtcagcaag
    38941 ccaatcggta aatctgccga caatatgtat agctatctgg cgctggtgca ggacgatccg
    39001 accgtgcaag tcgtgaacaa cgcgcaaaaa gcgtatgtcg aacattacat tcagggcgat
    39061 ccggatctgg caaaactgcc ggtgctttca gctgccgcac cgtttaaagt tggtggtcgc
    39121 aaaaatgacc cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt ccgtaatgcc
    39181 gccgatcttt atctctaccc caatacgctg attgtagtga aagccagcgg taaagaggtg
    39241 aaagagtggc tggagtgctc cgccggacag tttaaccaga ttgatcctaa cagcacgaaa
    39301 ccacagtcac tcatcaactg ggatggtttc cgcacttata actttgatgt tattgatggt
    39361 gtgaattatc agattgatgt tacccagccc gcccgttatg acggcgagtg ccagatgatt
    39421 aataccaatg cggaaaggat taagaacctg acctttaatg gcaagccgat tgatccgaac
    39481 gccatgttcc tcgttgccac caataactat cgcgcttacg gcggcaaatt tgccggtacg
    39541 ggcgacagcc atatcgcttt tgcttcaccg gatgagaacc gctcggtgct ggcagcgtgg
    39601 attgctgatg agtcgaaacg tgcgggggaa attcacccgg cggcagataa caactggcgt
    39661 ttagcaccga tagctgccga taagaaactg gatatccgtt tcgaaacttc cccgtcagat
    39721 aaagccgcag cgtttattaa agagaaaggg cagtatccga tgaataaagt cgcgaccgat
    39781 gatatcgggt ttgcgattta tcaggtggat ttgagtaagt aaaacacttc tttttcggcc
    39841 tataaatcat caatcgcatc cggcatttat tggcggatgc gatgctggcg catcttatcc
    39901 gccctacaag tcatacaccg taggccgcgc atccggcagt tatgccgcac gttcatcccg
    39961 caccgccaac acctcgggca aattcaactc aatccagtcc gccagtgcag caaccttttc
    40021 gctcacctgc tcgcccagcg gcgtgaggct gtattcaaca tgcggtggca ccaccggata
    40081 cgcgatacgg ttaagaaaac catcctgttc taacgcctgt aacgactgcg caagcatctt
    40141 ttcgctcacc ccgccgattt tgcgccgcag gtcgctaaag cgatgagtac cttcgcgtag
    40201 cgccaccaga atcaacaccc cccaacggct ggtgacgtgt ttcaacacct cgcgcgacgg
    40261 gcactgttcc gcaaagagat taccctcttt cagttgttgc gacaggctaa cctgactcat
    40321 ttcatactta cctttttgta cgtacttact aaaagtaagt ttaggtgtta gcgtatttaa
    40381 acacaagaca aaacgatgga gacttcccat gatcgctatt actggtgcca ctggccaact
    40441 tggtcactat attattgaat ccttgatgaa aacggttcct gccagccaaa tagtggctat
    40501 cgttcgtaat ccggcaaaag cccaggctct agcagcacaa ggcattaccg tgcgtcaggc
    40561 tgactacggc gatgaagccg cactgacatc tgcacttcag ggagtggaaa aactactgct
    40621 gatctcttcc agcgaagtgg gtcaacgtgc cccgcagcat cgtaatgtta ttaatgccgc
    40681 aaaggcggct ggcgtgaaat ttatcgctta taccagcctg ctacatgcag atacctcccc
    40741 gctcggcctc gccgatgagc acattgagac ggagaaaatg ctggctgatt ctggcatcgt
    40801 ttacaccctg ctgcgcaacg gctggtacag cgaaaactac ctcgccagcg ccccggcagc
    40861 actggaacac ggcgtattta tcggtgcggc gggcgatggc aaaatcgcct cagcaacgcg
    40921 ggcagattat gcggcagctg cggcacgcgt gattagcgaa gccggtcacg aaggcaaggt
    40981 ttacgaactg gcgggcgata gtgcctggac gttgacacag ttagcggcag agctgaccaa
    41041 acagagcggc aaacaggtta cctatcaaaa tctgagcgaa gccgatttcg ccgcggcact
    41101 gaaaagcgtc ggactgcccg acggactggc ggatatgctg gcggattctg acgttggcgc
    41161 atcgaaaggt ggtctgtttg ataacagcaa aacgcttagc aaattgattg gccgcccaac
    41221 gacaacgtta gccgaaagcg taagccatct ttttaatgtt aataactagt taattaaagt
    41281 ggcatcctcc cgcatcctct ctgataatga cgggatgccg ggagcaatca tgtctgcttc
    41341 ctgaactttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    41401 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt accggaatac
    41461 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctgcccatt
    41521 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gcgactggct taccgccttg
    41581 atgctcacca tgatgggcaa ccttatctat tacttctgcc ttgccagtgc cattcaacgt
    41641 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcccgttttt
    41701 gccaatctgc tttatagcca gcgcgacggc aaactcgcat ggggaaaact cgccccggca
    41761 ctggtttgta ttggcatcgg cctggcgtgt gtgaatattg ctgagttaaa ccacggactc
    41821 cccgattttg actgggcacg ttatacctct ggcatcgtgc tggcgttagt ttccgtggtc
    41881 tgctgggcgt ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    41941 ccgatgatgt gggcaacggc gcaggcgctg gtcacactgc cggtttctct catcggctat
    42001 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    42061 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    42121 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    42181 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    42241 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcattgc ggtcagagca
    42301 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacacgctgg cagtgagtta
    42361 aataagcctc tgctacgtaa gggttatagc ttttgcctta aagatgcatt taaaatacat
    42421 cttatattat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    42481 tggcgctctc tattcctcgc gcttcagctc tgtttcgtaa atatgatatg gattactgct
    42541 gtggcggtaa gcaaacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    42601 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    42661 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcaac
    42721 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    42781 tgccaaaagg gctgacaaaa tacctgacta tgctgcatga agagctatcc agccacatga
    42841 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    42901 gaccaatcag cgtgatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatta
    42961 aacacaccac caataacgtc acaccgccgc cggaagcctg caccacctgg aaagcgatgt
    43021 ataacggtat taatgaactg attgatgacc tgatggatca catcagcctg gaaaacaatg
    43081 tactgttccc acgcgcgctg gcgggtgagt aatctaaaag ctggatggca ttgcgccatc
    43141 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacaacc
    43201 agcccagccc cagtgcgata aaccacagcg gggtgaccaa cagcgcctgg cgagtgtcat
    43261 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    43321 gcttgccgag cggcatcttg tagatcgact tctcatgcag atgaggacgc tgtttgcggt
    43381 aaaccaggta cgagcaaagg ataattgtcc agacgaacat aaacagaatc gcggaaacgg
    43441 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    43501 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    43561 tagcgaacgc tttcggtgcc acaccttcct gcgccagacc aaacagcata cggctggtag
    43621 agaagacgcc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    43681 tggcagccgc aggcaaacct accaacacaa acagttcaac aaacgggctc ttctccggga
    43741 ctaccgaact ccacggcgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    43801 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    43861 ccgcagcggt tgtacctacc agctcaatcc ctacgaaagc gaaaaccgct atctggaatc
    43921 cggcaaagaa gccacttaaa cctttcggga accagccgcc atcattccac aaatgcgcga
    43981 atgacgcttc cacaccagtc ggtgactgaa agtgcatcgc caccataacc aggccgacga
    44041 caatcagcga cacaatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    44101 acattttcac ggtggcgaga ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    44161 cccagtcgga gagatcgggg aaccagaact gagcataagc agtgatcgcc accacgtctg
    44221 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cctgcccacg
    44281 gcccgagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    44341 attcccccat tgcccgcatc acgaaaaaga gcataaaacc gatgatcata taaacgaaaa
    44401 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac cccgtaccaa
    44461 tggcaccgcc aatggcaata agctgaatat gtcggtttgt gagattgcgc cgtagcgact
    44521 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg tttttttctt
    44581 cctgtacctg tctgtgttgt tcaggctcta cggcctttag tcagtctatg atacgacgaa
    44641 tctgtatcgg gactcatcga tattaggtaa caatacgcgg gatgaatact aagatttaga
    44701 tctaatgtta attttatgtt taaagtgagt gtcatatcac ggtgataacg cgaaacagct
    44761 cacaaaaata cacgcttacg gggtctttgc aacacaaaat agcgccgttt ggcaaactga
    44821 agggtttatt gctgaatgcc tgctcccctc tggttcgagg ggaacagaat gcgctgctta
    44881 gaggatttcc agcagttcga cttcaaacac cagggtgctg aacggaggga tggatgcgcc
    44941 tgcgccgcgc tcgccatatg ccagttcctg cgggatagtc agttcccatt tagaacctac
    45001 cggcatcaga gtcagtgctt caatccagcc agggatcacg ccattaaccg ggaattcagc
    45061 gggttcacca cgagcaacgg agctgtcaaa cacggtgccg tcgatcagtt taccggtgta
    45121 atgaacacga acgcggtcgg tacgtgccgg aattgcgcct tcaccctggt tgatcacgcg
    45181 gaattgcagg ccagattcgg tgctattcac accttctttt ttggcgtttt cttccaggta
    45241 tttcacacct tcagcagcca tcgcctggaa acgctgacga cgaacggcat cggcgcgttc
    45301 gtggatttca cgcagcgcgc gatgcaccac atcaaccggc acagccggat gtttgccttc
    45361 cagcgcatcg gcaatacctg caaccagtgc ttctggcagc agcccttcca ggccagattc
    45421 actcagttgt tgccctacct gcaaaccaat gccgtagctt gcttgcgctt cgatggtgtc
    45481 aaaagttggg gtggtcatcg tatttccttt cttagggtat aaaagtagcg ggcagcatat
    45541 cagccacact tctttgggta aaactttgtc gcaggaatga tgacaaatcc cggggaaaaa
    45601 gaaacaatag caatatcccg tatgaatata ataaaggcgg gtttaccacg gacatcatag
    45661 cgttagctta ttcataggct atgattgagg aacaagacgc ggagcaggag gaaaccatgc
    45721 ccgggcgctt tgaactaaaa ccaaccctgg agaaagtctg gcacgcgccg gacaattttc
    45781 gcttcatgga cccgctgccg ccgatgcatc gtcgggggat cattattgcc gccattgtat
    45841 tggtggtcgg atttctgctt ccatctgatg atacgcccaa cgcgccggtg gtgacgcgtg
    45901 aagcgcagtt agatattcag tcgcaatcac aaccgccgac agaagaacag ctgcgggcgc
    45961 aattagtcac accacaaaat gatcctgatc aggtggctcc ggtcgctcca gaacctatac
    46021 aggaaggcca gccagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    46081 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    46141 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    46201 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    46261 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    46321 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    46381 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctggggtg aaaccatact
    46441 gatgtgataa accaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    46501 aatattaata ttcacctgca atgggtcgat atctggctta aaaatattgt gtttcttccc
    46561 ttcatccaat aaggattcca ttaactgcaa gtgcgcatgg ttaatttcta agagacgcag
    46621 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    46681 agggtt
//
LOCUS       DAJTVJ010000015        45564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000015 DAJTVJ010000000
VERSION     DAJTVJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.015"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..355)
                     /locus_tag="OO448_000826"
     CDS             complement(<1..355)
                     /locus_tag="OO448_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6336121.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWA"
     gene            complement(369..1028)
                     /gene="pgmB"
                     /locus_tag="OO448_000827"
     CDS             complement(369..1028)
                     /gene="pgmB"
                     /locus_tag="OO448_000827"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6336122.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1025..3292)
                     /gene="ycjT"
                     /locus_tag="OO448_000828"
     CDS             complement(1025..3292)
                     /gene="ycjT"
                     /locus_tag="OO448_000828"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6336123.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGGSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3289..4344)
                     /locus_tag="OO448_000829"
     CDS             complement(3289..4344)
                     /locus_tag="OO448_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6336124.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4354..5142)
                     /locus_tag="OO448_000830"
     CDS             complement(4354..5142)
                     /locus_tag="OO448_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6336125.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5160..6212)
                     /locus_tag="OO448_000831"
     CDS             complement(5160..6212)
                     /locus_tag="OO448_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6336126.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6243..6846)
                     /locus_tag="OO448_000832"
                     /pseudo
     CDS             complement(6243..6846)
                     /locus_tag="OO448_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(6833..7714)
                     /locus_tag="OO448_000833"
     CDS             complement(6833..7714)
                     /locus_tag="OO448_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6336127.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7735..9045)
                     /locus_tag="OO448_000834"
     CDS             complement(7735..9045)
                     /locus_tag="OO448_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6336128.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9080..10590)
                     /locus_tag="OO448_000835"
                     /pseudo
     CDS             complement(9080..10590)
                     /locus_tag="OO448_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(10802..11116)
                     /gene="pspE"
                     /locus_tag="OO448_000836"
     CDS             complement(10802..11116)
                     /gene="pspE"
                     /locus_tag="OO448_000836"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6336129.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11191..11412)
                     /gene="pspD"
                     /locus_tag="OO448_000837"
     CDS             complement(11191..11412)
                     /gene="pspD"
                     /locus_tag="OO448_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6336130.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11421..11780)
                     /gene="pspC"
                     /locus_tag="OO448_000838"
     CDS             complement(11421..11780)
                     /gene="pspC"
                     /locus_tag="OO448_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6336131.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(11780..12004)
                     /gene="pspB"
                     /locus_tag="OO448_000839"
     CDS             complement(11780..12004)
                     /gene="pspB"
                     /locus_tag="OO448_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6336132.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12058..12726)
                     /gene="pspA"
                     /locus_tag="OO448_000840"
     CDS             complement(12058..12726)
                     /gene="pspA"
                     /locus_tag="OO448_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6336133.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            12893..13870
                     /gene="pspF"
                     /locus_tag="OO448_000841"
     CDS             12893..13870
                     /gene="pspF"
                     /locus_tag="OO448_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6336134.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(13988..15253)
                     /gene="puuE"
                     /locus_tag="OO448_000842"
                     /pseudo
     CDS             complement(13988..15253)
                     /gene="puuE"
                     /locus_tag="OO448_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15291..16571)
                     /gene="puuB"
                     /locus_tag="OO448_000843"
     CDS             complement(15291..16571)
                     /gene="puuB"
                     /locus_tag="OO448_000843"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6336135.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16573..18063)
                     /gene="puuC"
                     /locus_tag="OO448_000844"
                     /pseudo
     CDS             complement(16573..18063)
                     /gene="puuC"
                     /locus_tag="OO448_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18213..18770)
                     /gene="puuR"
                     /locus_tag="OO448_000845"
     CDS             complement(18213..18770)
                     /gene="puuR"
                     /locus_tag="OO448_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6336136.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(18797..19561)
                     /gene="puuD"
                     /locus_tag="OO448_000846"
     CDS             complement(18797..19561)
                     /gene="puuD"
                     /locus_tag="OO448_000846"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6336137.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            19773..21134
                     /gene="puuA"
                     /locus_tag="OO448_000847"
     CDS             19773..21134
                     /gene="puuA"
                     /locus_tag="OO448_000847"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6336138.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21283..21447
                     /locus_tag="OO448_000848"
     CDS             21283..21447
                     /locus_tag="OO448_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336139.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21437..22822
                     /gene="puuP"
                     /locus_tag="OO448_000849"
     CDS             21437..22822
                     /gene="puuP"
                     /locus_tag="OO448_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6336140.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            22956..23201
                     /locus_tag="OO448_000850"
     CDS             22956..23201
                     /locus_tag="OO448_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6336141.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23514..25157
                     /gene="sapA"
                     /locus_tag="OO448_000851"
     CDS             23514..25157
                     /gene="sapA"
                     /locus_tag="OO448_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6336142.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25154..26119
                     /gene="sapB"
                     /locus_tag="OO448_000852"
     CDS             25154..26119
                     /gene="sapB"
                     /locus_tag="OO448_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6336143.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26106..26996
                     /gene="sapC"
                     /locus_tag="OO448_000853"
     CDS             26106..26996
                     /gene="sapC"
                     /locus_tag="OO448_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6336144.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            26996..27988
                     /gene="sapD"
                     /locus_tag="OO448_000854"
     CDS             26996..27988
                     /gene="sapD"
                     /locus_tag="OO448_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6336145.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            27990..28796
                     /gene="sapF"
                     /locus_tag="OO448_000855"
     CDS             27990..28796
                     /gene="sapF"
                     /locus_tag="OO448_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6336146.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            28864..29217
                     /locus_tag="OO448_000856"
     CDS             28864..29217
                     /locus_tag="OO448_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6336147.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29585..30373
                     /gene="fabI"
                     /locus_tag="OO448_000857"
     CDS             29585..30373
                     /gene="fabI"
                     /locus_tag="OO448_000857"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6336148.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30518..31644
                     /locus_tag="OO448_000858"
                     /pseudo
     CDS             30518..31644
                     /locus_tag="OO448_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            31712..33646
                     /gene="rnb"
                     /locus_tag="OO448_000859"
     CDS             31712..33646
                     /gene="rnb"
                     /locus_tag="OO448_000859"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6336149.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(33724..33827)
                     /locus_tag="OO448_000860"
                     /pseudo
     CDS             complement(33724..33827)
                     /locus_tag="OO448_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33881..35871
                     /gene="pdeR"
                     /locus_tag="OO448_000861"
                     /pseudo
     CDS             33881..35871
                     /gene="pdeR"
                     /locus_tag="OO448_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36018..36191
                     /locus_tag="OO448_000862"
     CDS             36018..36191
                     /locus_tag="OO448_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336150.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36281..37030
                     /locus_tag="OO448_000863"
     CDS             36281..37030
                     /locus_tag="OO448_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6336151.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37299..37517
                     /gene="osmB"
                     /locus_tag="OO448_000864"
     CDS             37299..37517
                     /gene="osmB"
                     /locus_tag="OO448_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6336152.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(37643..37969)
                     /gene="yciH"
                     /locus_tag="OO448_000865"
     CDS             complement(37643..37969)
                     /gene="yciH"
                     /locus_tag="OO448_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6336153.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(37969..38706)
                     /gene="pyrF"
                     /locus_tag="OO448_000866"
     CDS             complement(37969..38706)
                     /gene="pyrF"
                     /locus_tag="OO448_000866"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6336154.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(38899..40068)
                     /gene="lapB"
                     /locus_tag="OO448_000867"
     CDS             complement(38899..40068)
                     /gene="lapB"
                     /locus_tag="OO448_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6336155.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40075..40383)
                     /gene="lapA"
                     /locus_tag="OO448_000868"
     CDS             complement(40075..40383)
                     /gene="lapA"
                     /locus_tag="OO448_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6336156.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40532..41296)
                     /gene="pgpB"
                     /locus_tag="OO448_000869"
     CDS             complement(40532..41296)
                     /gene="pgpB"
                     /locus_tag="OO448_000869"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6336157.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41466..42056
                     /gene="ribA"
                     /locus_tag="OO448_000870"
     CDS             41466..42056
                     /gene="ribA"
                     /locus_tag="OO448_000870"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6336158.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42120..44795)
                     /gene="acnA"
                     /locus_tag="OO448_000871"
     CDS             complement(42120..44795)
                     /gene="acnA"
                     /locus_tag="OO448_000871"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6336159.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(44959..45054)
                     /locus_tag="OO448_000872"
     CDS             complement(44959..45054)
                     /locus_tag="OO448_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336160.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45168..45335)
                     /locus_tag="OO448_000873"
     CDS             complement(45168..45335)
                     /locus_tag="OO448_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336161.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45338..45502)
                     /locus_tag="OO448_000874"
     CDS             complement(45338..45502)
                     /locus_tag="OO448_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6336162.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10770 a        12032 c        11471 g        11291 t
ORIGIN      
        1 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
       61 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
      121 ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
      181 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta ccgcagcctg
      241 acggaccgac aaacacgatg aactctttat cggcaatttc caggttgaag tccttcacca
      301 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
      361 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
      421 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      481 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      541 ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt cgagttttta
      601 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
      661 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
      721 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
      781 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
      841 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
      901 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
      961 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat tacctcttgc
     1021 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
     1081 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
     1141 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
     1201 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
     1261 gcaattcacc gtcacgcacg ctccccccgg caaaaccctg aatcgccccc agccagatag
     1321 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1381 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1441 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1501 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1561 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1621 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     1681 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     1741 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     1801 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     1861 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     1921 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     1981 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     2041 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     2101 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     2161 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     2221 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     2281 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     2341 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2401 ctaacgcttg ctgatcgtgc acttcgccgc catttaccgt gatacgacgt ttctgccacc
     2461 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2521 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2581 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     2641 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     2701 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     2761 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     2821 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     2881 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     2941 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     3001 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     3061 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     3121 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     3181 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     3241 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     3301 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3361 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3421 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3481 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3541 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3601 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     3661 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     3721 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     3781 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     3841 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     3901 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     3961 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     4021 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     4081 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     4141 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     4201 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     4261 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     4321 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4381 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4441 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4501 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4561 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4621 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     4681 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     4741 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     4801 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     4861 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     4921 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     4981 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     5041 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     5101 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     5161 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     5221 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     5281 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     5341 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5401 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5461 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5521 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5581 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     5641 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     5701 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     5761 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     5821 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     5881 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     5941 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     6001 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     6061 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     6121 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     6181 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     6241 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     6301 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6361 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6421 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6481 acggatatca gcccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6541 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6601 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     6661 tcagtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     6721 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     6781 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     6841 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     6901 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     6961 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7021 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7081 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7141 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7201 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7261 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7321 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7381 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     7441 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     7501 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     7561 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     7621 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     7681 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     7741 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     7801 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     7861 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     7921 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     7981 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8041 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8101 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8161 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8221 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8281 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8341 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     8401 gttaaagaca ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     8461 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     8521 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     8581 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     8641 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     8701 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     8761 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     8821 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     8881 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     8941 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9001 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9061 atttaatcca cataacctgt taggggcgca gcgttatttc attacccaat agatcaactt
     9121 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9181 cattaaacaa tccagtcaga caatcaccat cagcgttact tctttgaata cgcattacgg
     9241 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9301 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9361 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
     9421 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
     9481 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
     9541 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
     9601 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
     9661 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
     9721 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
     9781 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
     9841 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
     9901 ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
     9961 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10021 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10081 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10141 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10201 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10261 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10321 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10381 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    10441 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    10501 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    10561 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    10621 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    10681 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    10741 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    10801 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    10861 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    10921 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    10981 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11041 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11101 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11161 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11221 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11281 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11341 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    11401 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    11461 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    11521 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    11581 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    11641 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    11701 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    11761 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    11821 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    11881 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    11941 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12001 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12061 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12121 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12181 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12241 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12301 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12361 aatctctttc ttcatgcgtg ccagcgtatc gttcaccagc gtcacttcat gttccaggga
    12421 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    12481 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    12541 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    12601 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    12661 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    12721 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    12781 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    12841 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    12901 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    12961 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13021 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13081 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13141 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13201 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13261 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13321 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13381 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    13441 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    13501 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    13561 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    13621 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    13681 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    13741 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    13801 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    13861 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    13921 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    13981 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14041 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14101 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14161 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14221 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14281 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14341 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    14401 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    14461 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    14521 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    14581 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    14641 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    14701 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    14761 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    14821 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    14881 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    14941 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15001 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15061 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15121 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15181 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15241 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15301 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15361 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    15421 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    15481 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    15541 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    15601 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    15661 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    15721 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    15781 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    15841 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    15901 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    15961 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16021 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16081 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16141 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16201 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16261 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16321 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16381 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    16441 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    16501 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    16561 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    16621 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    16681 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    16741 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    16801 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    16861 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    16921 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    16981 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17041 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17101 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17161 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17221 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17281 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17341 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    17401 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    17461 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    17521 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    17581 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    17641 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    17701 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    17761 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    17821 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    17881 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct aggtgaccgg
    17941 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18001 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18061 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18121 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18181 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18241 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18301 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18361 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    18421 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    18481 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    18541 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    18601 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    18661 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    18721 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    18781 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    18841 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    18901 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    18961 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19021 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19081 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19141 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19201 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19261 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19321 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19381 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    19441 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    19501 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    19561 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    19621 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    19681 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    19741 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    19801 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    19861 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    19921 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    19981 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20041 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20101 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20161 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20221 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20281 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20341 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    20401 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    20461 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    20521 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    20581 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    20641 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    20701 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    20761 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    20821 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    20881 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    20941 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21001 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21061 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21121 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21181 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21241 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21301 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21361 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    21421 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    21481 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    21541 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    21601 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    21661 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    21721 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    21781 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    21841 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    21901 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    21961 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22021 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22081 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22141 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22201 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22261 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22321 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22381 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    22441 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    22501 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    22561 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    22621 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    22681 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    22741 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    22801 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    22861 cgcgccgcgc cattaactat aaaaagatgt ataacccgga tcacgagtgc tatcatccgc
    22921 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    22981 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23041 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23101 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23161 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23221 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23281 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23341 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    23401 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    23461 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    23521 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    23581 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    23641 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    23701 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    23761 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    23821 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    23881 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    23941 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24001 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24061 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24121 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24181 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24241 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24301 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24361 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    24421 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    24481 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    24541 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    24601 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    24661 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    24721 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    24781 cgcggttgat ggatatgagc catgatctga cattatccgg ttgggcgacg gacagtaacg
    24841 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    24901 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    24961 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25021 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25081 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25141 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25201 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25261 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25321 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25381 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    25441 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    25501 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    25561 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    25621 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    25681 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    25741 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    25801 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    25861 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    25921 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    25981 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26041 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26101 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26161 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26221 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26281 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26341 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    26401 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    26461 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    26521 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    26581 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    26641 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    26701 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    26761 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    26821 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    26881 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    26941 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27001 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27061 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27121 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27181 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27241 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27301 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27361 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    27421 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    27481 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    27541 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    27601 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    27661 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    27721 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    27781 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    27841 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    27901 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    27961 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28021 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28081 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28141 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28201 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28261 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28321 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28381 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    28441 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    28501 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    28561 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    28621 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    28681 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    28741 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    28801 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    28861 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    28921 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    28981 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29041 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29101 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29161 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29221 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29281 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29341 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    29401 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    29461 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    29521 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    29581 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    29641 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    29701 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    29761 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    29821 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    29881 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    29941 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30001 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30061 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30121 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30181 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30241 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30301 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30361 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    30421 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    30481 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    30541 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    30601 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    30661 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    30721 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    30781 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    30841 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    30901 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    30961 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31021 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31081 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31141 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31201 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31261 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31321 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31381 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    31441 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    31501 gcacccgatc gctttagcgc cgcccagttc agccctttac tcggacacga aaaatcatca
    31561 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    31621 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    31681 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    31741 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    31801 aaaaggcttt ggcttcctgg aagtcgacgc gaaaaaaagt tatttcattc cgccgccgca
    31861 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    31921 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    31981 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32041 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32101 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32161 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32221 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32281 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32341 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    32401 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    32461 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    32521 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    32581 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    32641 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    32701 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    32761 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    32821 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    32881 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    32941 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33001 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33061 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33121 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33181 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33241 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33301 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33361 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    33421 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    33481 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    33541 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    33601 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    33661 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    33721 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    33781 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    33841 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    33901 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    33961 cgatgttttg cgctttttta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34021 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34081 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34141 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34201 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34261 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34321 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34381 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    34441 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    34501 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    34561 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    34621 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc tgataacaat
    34681 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    34741 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    34801 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    34861 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    34921 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    34981 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35041 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35101 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35161 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35221 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35281 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35341 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    35401 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    35461 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    35521 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    35581 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    35641 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    35701 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    35761 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    35821 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    35881 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    35941 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36001 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36061 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36121 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36181 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36241 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36301 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36361 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    36421 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    36481 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    36541 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    36601 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    36661 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    36721 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    36781 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    36841 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    36901 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    36961 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37021 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37081 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37141 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37201 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37261 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37321 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37381 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    37441 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    37501 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    37561 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    37621 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    37681 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    37741 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    37801 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    37861 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    37921 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    37981 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38041 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38101 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38161 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38221 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38281 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38341 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    38401 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    38461 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    38521 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    38581 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    38641 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    38701 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    38761 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    38821 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    38881 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    38941 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39001 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39061 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39121 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39181 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39241 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39301 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39361 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    39421 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    39481 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    39541 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    39601 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    39661 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    39721 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    39781 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    39841 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    39901 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    39961 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40021 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40081 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40141 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40201 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40261 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40321 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40381 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    40441 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    40501 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    40561 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    40621 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    40681 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    40741 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    40801 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    40861 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    40921 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    40981 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41041 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcaaaaa cgcagacacc
    41101 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41161 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41221 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41281 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41341 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    41401 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    41461 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    41521 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    41581 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    41641 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    41701 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    41761 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    41821 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    41881 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    41941 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42001 acgaacatta tctcgatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42061 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42121 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42181 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42241 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42301 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42361 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    42421 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    42481 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    42541 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    42601 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    42661 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    42721 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    42781 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    42841 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    42901 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    42961 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43021 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43081 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43141 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43201 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43261 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43321 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43381 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    43441 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    43501 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    43561 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    43621 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    43681 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    43741 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    43801 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    43861 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    43921 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    43981 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44041 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44101 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44161 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44221 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44281 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44341 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    44401 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    44461 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    44521 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    44581 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    44641 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    44701 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    44761 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    44821 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    44881 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    44941 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45001 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45061 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45121 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45181 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45241 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45301 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45361 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    45421 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    45481 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    45541 caaatcctaa agaaacttta gtca
//
LOCUS       DAJTVJ010000016        44768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000016 DAJTVJ010000000
VERSION     DAJTVJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.016"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..666
                     /locus_tag="OO448_000875"
     CDS             <1..666
                     /locus_tag="OO448_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCR6336163.1"
                     /translation="LSQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQ
                     MIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVI
                     LFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADII
                     MACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDV
                     SQE"
     gene            736..1512
                     /gene="yaaA"
                     /locus_tag="OO448_000876"
     CDS             736..1512
                     /gene="yaaA"
                     /locus_tag="OO448_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6336164.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1665..1961)
                     /locus_tag="OO448_000877"
     CDS             complement(1665..1961)
                     /locus_tag="OO448_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6336165.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2175..3461)
                     /gene="thrC"
                     /locus_tag="OO448_000878"
     CDS             complement(2175..3461)
                     /gene="thrC"
                     /locus_tag="OO448_000878"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6336166.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(3462..4394)
                     /gene="thrB"
                     /locus_tag="OO448_000879"
     CDS             complement(3462..4394)
                     /gene="thrB"
                     /locus_tag="OO448_000879"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6336167.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4396..6858)
                     /gene="thrA"
                     /locus_tag="OO448_000880"
     CDS             complement(4396..6858)
                     /gene="thrA"
                     /locus_tag="OO448_000880"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6336168.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6885..7004)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6940..7005)
                     /gene="thrL"
                     /locus_tag="OO448_000881"
     CDS             complement(6940..7005)
                     /gene="thrL"
                     /locus_tag="OO448_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6336169.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7219..7905)
                     /locus_tag="OO448_000882"
     CDS             complement(7219..7905)
                     /locus_tag="OO448_000882"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6336170.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8305..8445)
                     /locus_tag="OO448_000883"
     CDS             complement(8305..8445)
                     /locus_tag="OO448_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336171.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8541..9257
                     /gene="arcA"
                     /locus_tag="OO448_000884"
     CDS             8541..9257
                     /gene="arcA"
                     /locus_tag="OO448_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6336172.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9317..10669)
                     /gene="creD"
                     /locus_tag="OO448_000885"
     CDS             complement(9317..10669)
                     /gene="creD"
                     /locus_tag="OO448_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6336173.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10727..12151)
                     /gene="creC"
                     /locus_tag="OO448_000886"
     CDS             complement(10727..12151)
                     /gene="creC"
                     /locus_tag="OO448_000886"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6336174.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(12151..12840)
                     /gene="creB"
                     /locus_tag="OO448_000887"
     CDS             complement(12151..12840)
                     /gene="creB"
                     /locus_tag="OO448_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6336175.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12853..13326)
                     /gene="creA"
                     /locus_tag="OO448_000888"
     CDS             complement(12853..13326)
                     /gene="creA"
                     /locus_tag="OO448_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6336176.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13537..14406
                     /gene="robA"
                     /locus_tag="OO448_000889"
     CDS             13537..14406
                     /gene="robA"
                     /locus_tag="OO448_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6336177.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14403..15050)
                     /gene="gpmB"
                     /locus_tag="OO448_000890"
     CDS             complement(14403..15050)
                     /gene="gpmB"
                     /locus_tag="OO448_000890"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6336178.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15102..15623
                     /gene="yjjX"
                     /locus_tag="OO448_000891"
     CDS             15102..15623
                     /gene="yjjX"
                     /locus_tag="OO448_000891"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6336179.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15708..16034)
                     /gene="trpR"
                     /locus_tag="OO448_000892"
     CDS             complement(15708..16034)
                     /gene="trpR"
                     /locus_tag="OO448_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6336180.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16124..18061)
                     /gene="sltY"
                     /locus_tag="OO448_000893"
     CDS             complement(16124..18061)
                     /gene="sltY"
                     /locus_tag="OO448_000893"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6336181.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18272..19939
                     /gene="ettA"
                     /locus_tag="OO448_000894"
     CDS             18272..19939
                     /gene="ettA"
                     /locus_tag="OO448_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6336182.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20246..21478)
                     /gene="nadR"
                     /locus_tag="OO448_000895"
     CDS             complement(20246..21478)
                     /gene="nadR"
                     /locus_tag="OO448_000895"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6336183.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21499..22881)
                     /gene="radA"
                     /locus_tag="OO448_000896"
     CDS             complement(21499..22881)
                     /gene="radA"
                     /locus_tag="OO448_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6336184.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22930..23898)
                     /gene="serB"
                     /locus_tag="OO448_000897"
     CDS             complement(22930..23898)
                     /gene="serB"
                     /locus_tag="OO448_000897"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6336185.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            24004..24648
                     /locus_tag="OO448_000898"
     CDS             24004..24648
                     /locus_tag="OO448_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6336186.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24676..25692
                     /gene="lplA"
                     /locus_tag="OO448_000899"
     CDS             24676..25692
                     /gene="lplA"
                     /locus_tag="OO448_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR6336187.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25724..25873
                     /locus_tag="OO448_000900"
     CDS             25724..25873
                     /locus_tag="OO448_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336188.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(26148..26867)
                     /gene="deoD"
                     /locus_tag="OO448_000901"
     CDS             complement(26148..26867)
                     /gene="deoD"
                     /locus_tag="OO448_000901"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6336189.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26947..28170)
                     /gene="deoB"
                     /locus_tag="OO448_000902"
     CDS             complement(26947..28170)
                     /gene="deoB"
                     /locus_tag="OO448_000902"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6336190.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(28222..29544)
                     /gene="deoA"
                     /locus_tag="OO448_000903"
     CDS             complement(28222..29544)
                     /gene="deoA"
                     /locus_tag="OO448_000903"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6336191.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(29671..30450)
                     /gene="deoC"
                     /locus_tag="OO448_000904"
     CDS             complement(29671..30450)
                     /gene="deoC"
                     /locus_tag="OO448_000904"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6336192.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30708..32258
                     /locus_tag="OO448_000905"
     CDS             30708..32258
                     /locus_tag="OO448_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6336193.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            32230..33093
                     /locus_tag="OO448_000906"
     CDS             32230..33093
                     /locus_tag="OO448_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6336194.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(33508..34290)
                     /locus_tag="OO448_000907"
     CDS             complement(33508..34290)
                     /locus_tag="OO448_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6336195.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(34287..35360)
                     /locus_tag="OO448_000908"
     CDS             complement(34287..35360)
                     /locus_tag="OO448_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6336196.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(35482..35643)
                     /locus_tag="OO448_000909"
     CDS             complement(35482..35643)
                     /locus_tag="OO448_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6336197.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(35770..36375)
                     /gene="osmY"
                     /locus_tag="OO448_000910"
     CDS             complement(35770..36375)
                     /gene="osmY"
                     /locus_tag="OO448_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6336198.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(36768..38354)
                     /gene="prfC"
                     /locus_tag="OO448_000911"
     CDS             complement(36768..38354)
                     /gene="prfC"
                     /locus_tag="OO448_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6336199.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVS
                     RLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            38574..38793
                     /locus_tag="OO448_000912"
                     /pseudo
     CDS             38574..38793
                     /locus_tag="OO448_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            39038..39589
                     /locus_tag="OO448_000913"
     CDS             39038..39589
                     /locus_tag="OO448_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HCR6336200.1"
                     /translation="MHENIWFTYKARIQAHHRLEWLEKHSQFILVWYAILSAVLSIIT
                     LRFPKFLGDNTDIVAAILSVALLGISLIVSNLDYRGRAIAMRRNYIALQRLYFDINNG
                     QQLTLEQKEKYFNLLNEVENHRDIDDKVSRVTQVGLTTRLPTQKEKIIVKLWILRRMF
                     ITVGLYILPLICLWIDYECKQSF"
     gene            39567..40937
                     /locus_tag="OO448_000914"
     CDS             39567..40937
                     /locus_tag="OO448_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR6336201.1"
                     /translation="MNASRVFKKSFSKKNLLKVYNEKIKESGAIGIDRIRPSKLDLTI
                     KNEITFIFEKVNCGNYKFTAYKEKLISKGANSIPRQISIPTARDRITLRALCECLTEI
                     YPNSRLKLPHTVIDLLKNALNSDLYAEYAKIDLKSFYPSIEHKLIFNAIKNKIRKKEI
                     RQLITSSLIVPTVSGSTGSKGVPNNTRGVPQGLAISNILAEISLSDFDNEINKMHDIW
                     YMRYVDDILILTQKDQATKIASHIIDKLQSLNLNPHPLNEENSKSKVGSLDESFNFLG
                     YHIKNRELLIKHESILRFESSLAKIFTAYRHALLQAKNKRDKERAVAYCQWKLNLRIT
                     GCVFEGKRLGWVSYFSQITSTAQLRAVNHTINNLIRRFGLSSEIKPKSLIKTFYELRR
                     GRAETFKYIPNFDNLHISQKREIVSMWIGKENEKKLSNSEIERKFKFKIAKSVKELEE
                     DISGIS"
     gene            complement(41050..41634)
                     /locus_tag="OO448_000915"
     CDS             complement(41050..41634)
                     /locus_tag="OO448_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6336202.1"
                     /translation="MAFRMSEQARTIKIYNLLSGTNEFIGDGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEYQKWNGTAWLKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAVDLEIATEEE
                     NSLLEAWKKYRVLLNRVDTSTAPDIEWPAVPVME"
     gene            complement(41634..>44768)
                     /locus_tag="OO448_000916"
     CDS             complement(41634..>44768)
                     /locus_tag="OO448_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6336203.1"
                     /translation="TEDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSASDASTS
                     AREAATHATDAAGSARAASTSAGQAASSAQSASSSAGTASTKASEASKSAAAAESSKS
                     AAATSAAAAKTSETNAAASQQSAATSASAATTKASEAATSARDAAASKEAAKSSETNA
                     SSSASSAASSATAAGNSAKAAKTSETNARSSETAAGQSASAAAGSKTAAASSASAAST
                     SAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASE
                     TSAESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATA
                     AAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSETLAAT
                     PKAVKSAYDNAEKRLQKDQNGADIPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAG
                     ATSGKYYPVVVMRSAGSVSELASRVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRY
                     AYGMFWQYQNNERAIHSIMMSNKGDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLV
                     VNDTTYKFGATNPATECIAADVILDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVAR
                     GGNQIRMIGGEYGALWRNDGAKTYLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKL
                     SASLNGNALTATKLQTPRRVSGVEFDGSKDITLTAAHVAAFARRATDTYADADGGVPW
                     NAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWA
                     EVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDM
                     RGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAH
                     THSLSGSTGSAGAHTHGNGIRWPGGGGSALAFYDGGGFTYVHDSQYQVSPGTSSSRSY
                     YQRIQTQSAGAHTHSLSGTAASSGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAEN
                     TVKNIAFNYIVRLA"
BASE COUNT        10861 a        12022 c        11061 g        10824 t
ORIGIN      
        1 ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga ggcgggaatg
       61 ggttcgacgc caaacgcggc agcggcagcg gcgtcctggc ctccgcatcc ggcagcgcaa
      121 gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc
      181 atgctgatat tactggcggg taacgacaca acctacatgc cgctggaagg tattcagctt
      241 atccagaagg cgatgcgggt gctaatgggt tcctggggtg ctgagtttgt taccctcgtg
      301 gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc
      361 ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg caccttcgca
      421 acggtcatcg gcggcacctt gctaagtctt ccgctgatgt ggcaactggc agatatcata
      481 atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc tgtggttcat
      541 accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg
      601 ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga tgtttcgcag
      661 gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc
      721 ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga ttaccaaagc
      781 ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca gcacttgatc
      841 catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat cagcgacaaa
      901 ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac gccggagaat
      961 gcccgccagg ctattttggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaact
     1021 ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat
     1081 ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt
     1141 gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg
     1201 aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc agatgagtat
     1261 tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat
     1321 gagaagaacg gcaaatttaa gataatcagc ttctacgcta agaaagcacg cggtctgatg
     1381 agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc
     1441 gaaggttact tctttgatga agcttcctcc agcaatggcg aactggtgtt taaacgctac
     1501 gagcagcgtt aagtttgcag tcaatgccgg atgcggcgtg aacgccttat ccggcctaca
     1561 aaagcatgca gattcaatat attgcagaat tcacgtaggc ctgataagac gcgccagcgt
     1621 cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg gtgatgtttg
     1681 cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg cggcggtggc
     1741 ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt ccaccagccg
     1801 tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc gcctatctgt
     1861 aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg agcgaccaga
     1921 accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc cttttatcat
     1981 tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg ttattctccg
     2041 caatcctaat ctctaagtta ccgcatttat tgagaatttc tcctttttcc ctgtcgtttt
     2101 tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg cctgattgag
     2161 ataatgaata gattttactg atgattcatc atcaatttac gcaacgcagc aaaatcggcg
     2221 ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt tggcagatcc
     2281 aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg cgcggtgccg
     2341 aggaacaagc catattcgcc tggattcaac tggtcacaca gcgcacgata cgctacggca
     2401 gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg ttgcgtggtt
     2461 tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg gcggaacaac
     2521 tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga taacgtcgcc
     2581 tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt atcgttcacg
     2641 ttggtcgcag caataaaacg tttcaccggc agaccgagtg acttcgccag cagacccgcc
     2701 gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg cgcttcctgc
     2761 ggcagctgcg caactgcttc aaagtagtag caaatctgcg ccagcaaacg gctgatgtta
     2821 atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc aaacgcctgc
     2881 ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc gatattgccg
     2941 cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg atagaggata
     3001 accactttca cattcggcaa accgtagaaa gcatgagcca ctgccgctcc ggtatcaccg
     3061 gaggtcgcgg tcagaatggt cactggctta tcgcccgcaa tatgggtcag catttgtgcc
     3121 ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa ttccagacaa
     3181 ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc gcgcacgcgc
     3241 tcttccagga tttcctgcgg gatttcatca ccaataaacg ccgagaggat cttcgcactg
     3301 cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa ttccggcagg
     3361 tcatgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc ttgcgcaaag
     3421 ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc cagtactcgt
     3481 gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg caggtagttc
     3541 ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag agcgaacaag
     3601 gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac cgcctgccgc
     3661 gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac atctttcatc
     3721 agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc agccagatgt
     3781 cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc ccgggcttct
     3841 gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc aaaccctggc
     3901 acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc aagaaaacac
     3961 ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc gcccatcaaa
     4021 gccagcaaac gggtgtcatt aagtggcttg ccgcagtgtt cattcatcgc catcagcgcc
     4081 gcgaccaccg aacaggcgct ggagcctaag cccgagccga tcggcatatt cttttccaga
     4141 gtcatcgcca ctggaatttg cttgcccagc tccaggcaaa aacgctccca gcactgataa
     4201 acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag gttgttgaga
     4261 ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc atcaacgggt
     4321 gtcaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga agccggggca
     4381 taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca gatcggcaaa
     4441 gacaccagca gcggtaacgt cattgcccgc accatatccg cgcagcacca acggcagcgg
     4501 ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt tgaacagcgg
     4561 atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat caatattgcc
     4621 aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg caaagagatc
     4681 gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt taaactctgc
     4741 gggcagcaca ggttcaattt caatatccgc cagttccagt tcacgtcccg tttcacgagc
     4801 gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg ggtccggttc
     4861 ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc cttcgtctaa
     4921 cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca ccaattcatc
     4981 acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc caacgttggt
     5041 gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat gatagtaatc
     5101 catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt cgcgcaggaa
     5161 gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga ccgggttcag
     5221 cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct ctttggcttg
     5281 tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga gcagagcctt
     5341 cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc agctttgctg
     5401 acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca caaacacttc
     5461 gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag tggtcgcatc
     5521 atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa tggcgacaat
     5581 gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac gcaaggtgcg
     5641 cataccatca cctaccaccg agataatggc cagccgttcc atcaccgcca gcggctccag
     5701 taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag cccgcacaca
     5761 gtcgctttgc ggaacgcaga aactgatact gtattcggaa gatgattgcg taatcagcac
     5821 cacggaaata cgggcgcgtg acatcgcggc aaagacgcgc gccgccatgc cgaccattcc
     5881 tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg aaatgccctt
     5941 gaccggtaat tcgccttcat cacgactggc accaatgagc gtacctggtg cttgaggatt
     6001 tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa tggtgcgggg
     6061 gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg acatcgactt
     6121 caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt caacgtccgt
     6181 ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg agtagtcgga
     6241 accgttgcgt ccaagtacca ccagttcgcc tttttcatta ccggcggtga agcctgccat
     6301 cagcaccatg tgatcagccg gaatacgact tgccgcaata cggcgggtgg actcagcaat
     6361 atcgacggta gattcgaggt aatgccccac tgccagtagt ttttcgaccg gatcgataac
     6421 ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg acattttctc
     6481 gccacggcaa atcagcgcag cgttaatgct atccgggcac tgccctaaca aactaatgcc
     6541 atgcaggaca tgttttattt gggcaaattc ctggtcgacg aaagttttca attgcgccag
     6601 cgggaatccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa tacgttcggc
     6661 atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca tcgccaccag
     6721 gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct gcctggcatt
     6781 gctttccaga atatcggcaa cccgcagaaa acgttctgca tttgccactg atgtaccgcc
     6841 gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa aaagcccgca
     6901 ctgtcaggtg cgggcttttt tctgtgtttc ctgtacgcgt cagcccgcac cgttacctgt
     6961 ggtaatggtg atggtggtgg taatggtggt gctaatgcgt ttcatggatg ttgtgtactc
     7021 tgtaattttt atctgtctgt gcgctatgcc tatattggtt aaagtattta gtgacctaag
     7081 tcaataaaat tttaatttac tcatggcagg taaccagttc agaagctgct atcagacact
     7141 ctttttttaa tccacacaga gacatattgc ccgttgcagt cagaatgaaa agctgaaaaa
     7201 tacttactaa ggcgtttttt atttggtgat atttttttca atatcatgca gcaaacggtg
     7261 caacattgcc gtgtctcgtt gctctaaaag ccccaggcgt tgctgtaacc agtcgaccag
     7321 ttttatgtca tctgccactg ccagagtcgt cagcaatgcc atgactcgtt cgcgtaaagc
     7381 ttgcagttga tgttggtctg ccgttgtgtc acttttcgcc ggttgttgta ttaatgttgc
     7441 taattgatag caatagacca tcaccgcctg ccccagattg agcgaaggat aatccgccac
     7501 catcggcaca ccagtaagaa cgtcagccaa cgctaactct tcgttagtca gcccggaatc
     7561 ttcgcgacca aacaccagcg cggcatggct catccatgaa gatttttcct ctaacagcgg
     7621 caccagttct actggcgtgg cgtagtaatg atatttcgcc cgactgcgcg cagtggtggc
     7681 gacagtgaaa tcgacatcgt gtaacgattc agccagtgtt gggaaaactt taatattatc
     7741 aataacatca ccagatccat gtgcgaccca gcgggtggct ggctccaggt gtgcctgact
     7801 atcgacaatc cgcagttcgc taaaacccat cgttttcatt gcccgcgccg ctgccccaat
     7861 attttctgct ctggcgggtg cgaccaggat aatcgttata cgcatattgc cactcttctt
     7921 gaccaaataa ccgcgaacag ggtgatcact gtcaacttat tacgcggtgc gaatttacaa
     7981 attcttaacg taagtcgcag aaaaagccct ttacttagct taaaaaaggc taaactattt
     8041 cctgactgta ctaacggttt aattgttaaa aaatgctaca tatccttctg tttacttagg
     8101 ataattttat aaaaaataaa tctcgacaat tggattcacc acgtttatta gttgtatgat
     8161 gcaactagtt ggattattaa aataatgtga cgaaagctag catttagata cgatgatttc
     8221 atcaaactgt taacgtgcta caattgaact tgatatatgt caacgaagcg tagttttatt
     8281 gggtgtccgg cccctcttag cctgttatgt tgctgttaaa atggttagga tgacagccgt
     8341 ttttgacact gtcgggtcct gagggaaagt acccacgacc aagctaatga tgttgttgac
     8401 gttgatggaa agtgcatcaa gaacgcaatt acgtacttta gtcatgttac gccgatcatg
     8461 ttaatttgca gcatgcatca ggcaggtcag ggacttttgt acttcctgtt tcgatttagt
     8521 tggcaattta ggtagcaaac atgcagaccc cgcacattct tatcgttgaa gacgagttgg
     8581 taacacgcaa cacgttgaaa agtattttcg aagcggaagg ctatgatgtt ttcgaagcga
     8641 cagatggcgc ggaaatgcat cagatcctct ctgaatatga catcaacctg gtgatcatgg
     8701 atatcaatct gccgggtaag aacggtcttc tgttagcgcg tgaactgcgc gagcaggcga
     8761 atgttgcgtt gatgttcctg actggccgtg acaacgaagt cgataaaatt ctcggcctcg
     8821 aaatcggtgc agatgactac atcaccaaac cgttcaaccc gcgtgaactg acgattcgtg
     8881 cacgcaacct gctgtcccgt accatgaatc tgggtactgt cagcgaagaa cgtcgtagcg
     8941 ttgaaagcta caagttcaat ggttgggaac tggacatcaa cagccgttcg ttgatcggcc
     9001 ctgatggcga gcagtacaag ctgccgcgca gcgagttccg cgccatgctt cacttctgtg
     9061 aaaacccagg caaaattcag tcccgtgctg aactgctgaa gaaaatgacc ggccgtgagc
     9121 tgaaaccaca cgaccgtact gtagacgtga cgatccgccg tattcgtaaa catttcgaat
     9181 ctacgccgga tacgccggaa atcatcgcca ccatccacgg tgaaggttat cgcttctgtg
     9241 gtgatctgga agattaatcg gctttaccac cgtcaaaaaa aacggcgctt tttagcgccg
     9301 tttttatttt tcaaccttat ttccagatac gtaattcatc gtccgttgta acttctttac
     9361 tggctttcat tttcggcagt gaaaacgcat accagtcgat attacgagtc acaaacatca
     9421 tgccggccag cgccaccacc agcacactgg ttcccaacaa cagcgcgcta tcgacagagt
     9481 tgagcagtcc ccacatcact ccatccagca acaacagcgc gagggtaaac aacatgctat
     9541 tgcgccagcc tttcaatacc gcctgcaaat aaataccgtt cattaacgcc ccaatcagac
     9601 tggcgattat ccatgccacg gtaaaaccga tatgttcaga aagcgccagc aagagcaaat
     9661 aaaacatcac caatgaaagc cccaccagca aatattgcat tgggtgtaaa cgttgcgcgg
     9721 tgagcgtttc aaaaacaaag aacgccataa aagtcagtgc aatcagcaga atggcgtact
     9781 tagtcgcccg gtccgttaat tggtattgat cggctggcgt cgtcactgcg acgctaaacg
     9841 cgaggaagtt ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg agattattag
     9901 caaaccagct gctttgccat tgcgcctgaa aacctgactc gctaacttcc cgtttggctg
     9961 gcaaaaaatc acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc atttcgctat
    10021 tacgcccggc aggcaccaca gaaagatcgc cggtaccgct taaattcagg gccatattca
    10081 gcttcaggtt ctgcttccgc cagtcccctt cgggtaaagg gatatgcacg ccctgcccgc
    10141 cttgctctaa cccggtgccg ggttcaatgg tcagcgccgt tccgttaact tcaggcgctt
    10201 tcaccacacc aataccacgc gcatccccga cgctaatcac aataaatggc ttgccaaagg
    10261 tgatatttgg cgcgtcgagt tcgctaagac gcgaaacatc gaaatcggct tttagcgtta
    10321 aatcactgtg ccagacctga ccggtataaa tccctatctt gcgttcttcc acgttctgat
    10381 tgccatcaac catcaatgac tcaggtaacc aaaaatggat aaaacttcgt ttccgctcca
    10441 cggttttatc ctcttcctgt accgtataaa gctcggtcac aggaatagcg ataagcggcc
    10501 caacaagttt ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca tcgctacggt
    10561 aatcggcacg ttcgacaatc acctgccgaa tcagcattat cggaatcaac aacagcaata
    10621 ctgcaccaaa cagggtggtc attttccaga acagggagga tttcaacata gtcttctcct
    10681 ttgcaatggc agcaggatac gaaggctatg tgggaagaat ttgaagctat gtgaagtgac
    10741 ggtgaagtcg aagcgaggcc agcacgccac cttcctgcac gttgcgcagc gtgacttcgc
    10801 cgttaaacaa acgggcgacc tcactgacga acgccaaccc cagaccgctg cttttttgcc
    10861 catttgcacg cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg tagtcaggaa
    10921 taccactacc ggtatccagc accttaagcg tgacgtgttc ctgatccact tcggcgctta
    10981 gcgttatacg accgctctcg ggggtaaaat cgatggcgtt atccagtaaa ttccccagcg
    11041 cctgatccag taacgccggt tcagcagcaa cattaacctc ggtgggcgta acatgtagag
    11101 tgatgttttt ttctgccaac tgcacggtgc gcgcttcgct gacgcggcgg aataatgccg
    11161 ccacatcaac agcagtcaaa acgacttcct gacgattctc cagtcttgcc tggcgtagta
    11221 acgtttccac cagtgcctgc attcgcgcat tttgcgtcag aatgttgtca gtaaaacgag
    11281 ccactacttc aggcggcgga ccttcgcgta aaatttccgc cgcgccacga atcgccgcca
    11341 gtgggctttt tagctcatgg gttaacgcat aaacatactg ctcaatatag tttttccctt
    11401 ccagcttcac gcgcatactt tccagcgcct gcgcgagttt acgcagctcg ctactaccga
    11461 gatcgggaag aggaacgggc ttattgtcag tgacggaatc agcatagcga gtgagccggg
    11521 caatggagcg gttgatccac caaaccatgc ctgcgccaat caccagtgcg atccccaaca
    11581 aaatggcgct ggcccataaa attcgccgct cgctgcgttt aatgaccgga gccatcgccg
    11641 cgttcggttt gcctacgctc aaaacgccaa taagccgcga gccgtccata atcggcgcgg
    11701 caacatacat caccgaactt tcgggatcgg caggattttg caacgtgcta cgcgcaccat
    11761 actgaccacg caacgttagc cagacgtcat tccagcgcga ataatcctgc ccaacggctt
    11821 tatttgccga atcgaacaat actttgccct gcgcatcggt catatagaca tgatattcat
    11881 tgcgcacttt gttaatgcca ccgatattgg cgcgaaacgg gcgatgttgt agctgattaa
    11941 acgcctgagc cagttgccca tgcgttgggt ccccagagag caaatcggga cgcgccagct
    12001 ccgccagcaa cgttgcggta tcgattaacg tcccctccgt tgctcttcgc acgcccggtt
    12061 taacttcttt gacaaaaata gccagtacga accaggccgc caccgccacc agtaaaaaat
    12121 agcccagcag caaccgcatg ccgatacgca ttacaggccc ctcaggctat atcccatgcc
    12181 gcgatgagta ttaatcggag aaagatcggg gttgatggcg cgcagcttgg cacgcaacgt
    12241 tttaatgtgg gtatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc acacgctatc
    12301 cattagttgc tggcgggacc agacgcggcc tggcgacttg agtaacgtct tcagcaataa
    12361 aaactcatac cgcgtcagcg ttaatggcgt gtcaaaccag ctgatctgcg ccgcgggttc
    12421 attcaattca aaatgaccaa tacggatgac gggagacggc gacgagaact tcttcacccg
    12481 acgcagtaag gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt tagccacata
    12541 gtcgtcagcg ccaatttcca gcccaagcag gcgatcgacc tcttcacttc gggccgtcag
    12601 gaacagtaca ggtaacgccg gatggagcgc cagtaactgg cggcacaatt caaagccgct
    12661 gatatcaggc aaaccaacat cgagaatcat gacgtcggga acctgctggc gagctttatc
    12721 cagcaccggc aagcctcgct caaagacctc gacggcaaaa ccttcctgct gcaacatgta
    12781 gaccagcgtg tcggctatcc cttgctcatc ttccactaac cagaccgttt cccgttgcat
    12841 aaatcgcctc tgttattgcc gccacggcat gacaggaacc gcgctaatcg cgtttttcgg
    12901 cgaaccttct acaactttgt cggagtaagc cagataagcg agcgcgttgc gtttggcgtc
    12961 ataaaagcgc acgacctgta acgacttaaa gaccagggac gtgcgttttt tgaatactac
    13021 ctcgccctga gctttgccgt ttttaatacg atccgacagt tcaatcggcc cgacttgctg
    13081 acaagaaata gccgcatcgg aggtatcttc cgccagaccc aatccccctt taataccacc
    13141 tgttttcgcc cggctcacat aacaggtgac atttttcaca tcgggatcgt caaaggcttc
    13201 cacaacaatt ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg aaccaatctc
    13261 ttctgcatga gccaatggcc ccagcattat caggcttaaa gacaggatca aatgcttgta
    13321 tttcatattg ttaccatttt tatctattac acgagcaatt agttcgtcac ggtaaataac
    13381 atattgatta agaacaaaat ctcaatactt ttatttccgt caagccctaa aacatactct
    13441 actaagaaaa aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca attacctgat
    13501 gtcaggtgct cgttgttgaa aggatgagga tattttatgg atcaggccgg cattattcgc
    13561 gaccttttaa tctggctgga aggtcatctg gatcagcccc tgtcgctcga caatgtagcg
    13621 gcgaaagcag gttattccaa gtggcactta cagagaatgt ttaaagatgt cactggccat
    13681 gctattggcg cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc actacgcctg
    13741 actgcgcgtc cgattctgga catcgcgctg caataccgct tcgactctca acagacattt
    13801 acccgcgcat tcaagaagca gtttgcccag actcctgcac tttaccgccg ttctcctgaa
    13861 tggagcgcct ttggtattcg cccgccgcta cgtctgggtg aattcacaat gccagagcac
    13921 aaatttgtca ccctggaaga tacgccgctg attggtgtta cccagagcta ctcctgttcg
    13981 ctggagcaaa tctctgattt ccgccatgaa atgcgttatc agttctggca cgattttctc
    14041 ggcaacgcgc cgaccattcc gccagtgctc tacggcctga atgaaacgcg tccgagtcag
    14101 gataaagacg acgaacaaga ggtattctat accaccgcgt tagcccagga tcaggcagat
    14161 ggctatgtac tgacggggca tccggtgatg ctgcagggcg gcgaatatgt gatgtttacc
    14221 tatgaaggtc tgggaaccgg cgtgcaggag tttatcctga cggtatacgg aacgtgcatg
    14281 ccaatgctca acctgacgcg ccgtaaaggt caggatattg agcgatacta cccggcagaa
    14341 gacgccaaag cgggagatcg cccaattaat ctgcgctgtg aactgctgat tcctatccgc
    14401 cgttaacgct gcagctcatc taatgcaggg gcgtccagat gcgagatgtc ccctgcggtt
    14461 tcaacgaccc agccagacgc cagccacagg ctttcctgat aatccacgcg cgaaatagaa
    14521 cagttacgca gacgtaagcg gcgctctgcc catgctggta atccgagaat cgtactcacc
    14581 agacatccca gtgcaatacc atggctcacc agcagcggtc ggcttccctg cggtaagtcc
    14641 cggcaagatt ccagtgccgc attgacgcga tcgctgagct cctgcattga ctcgccttca
    14701 ggaatacgcc cgtcaacggt gccattgacc agctgccgac gccagttctc ttcttcttcg
    14761 gtcagagaat cgatatgtct tttctccagc acacccatgt ttaattcacg caggcgagaa
    14821 tcaaagatga tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg ccgggtgcgt
    14881 cctaaatcgc tactgataat atgcgtaatg ccaagctctt tggcacgggt tgccacctgc
    14941 atcgcctgtt gctcaccttt ggcggtcagc gggctgtcag actggccctg aatacgtcgc
    15001 tcggcgttcc actgcgtttc accgtggcgg actaggtata cctgtaacat gctttttttc
    15061 cgtatactgc tgtaacgtta attttctgag taatgctgat tatgcaccaa gttatctgtg
    15121 cgaccaccaa tcccgctaaa attcaggcca ttctgcaggc atttcacgag atcttcggcg
    15181 aaggatcctg ccatattgca tccgttgccg tcgagagcgg tgtaccggaa cagccctttg
    15241 gcagtgagga aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc cgtttacttc
    15301 cagaggctga tttttgggtg gcgattgaag ctggcatcga tggtgacagc actttcagtt
    15361 gggtggtgat tgaaaacacc agccagcgcg gcgaggcgcg ttcggctact ttgccgttac
    15421 ctgcggtgat tctgcagaaa gtgcgtgaag gtgaggcgct tggtcccgtg atgtcgcgtt
    15481 ataccggtat tgatgagatt ggtcgtaagg aaggggcgat cggcgtgttt accgccggga
    15541 aactcactcg cgccagcgtc tatcaccagg cggtgatcct ggcgctgagt ccgtttcaca
    15601 atgccgtgta tcaggcattg taggacggat aaggcgttca cgccgcatcc ggcattcggt
    15661 gcacgatgcc tgatgcgacg ctgccgcgtc ttatcaggcc tacaaaatca atcgcttttc
    15721 agcaacacct cttccagcca ctggcgcagc tccacgggcg cggctttcag gctgttagat
    15781 ccacgcgtaa tcgtcgcgat gcccgcgccg agttcatttt ttaactcacg ctgactcatt
    15841 tcgccacgca acagctcttc gacaatacgc acgcgagtcc ccaacgcttc gcgctcatct
    15901 ggcgtcagca tcaggtttaa caacggtaaa tggagatcgt tttggtaggc attcttaagc
    15961 aggtcgacaa aacgtaacca ctcctggtga cgctgttctg ccatcgctgc tgaatagggt
    16021 gattgttggg ccataatatg tcgccattgt tagcggggga agcaaaatgc ctcccccggt
    16081 tgtactcgct aaagagtacg atagcatatc ataaacgtgc ggatcagtaa cgacgtcccc
    16141 attccgtggc gctcatcaac gtcggtttat cccccatgaa ataacggtag taagcgtcat
    16201 aagccagcac gttcttcaca taaccgcgcg tctcggagaa cggaatactc tcgacaaatg
    16261 ccacagcgtc gatacgcccg gcgctgttgc caagccaggt tcgcacccgt ccaggtccgg
    16321 cgttataagc tgctgaggag aaaatacggt tattgccaaa ctgctgataa acatattgca
    16381 ggtaactggt gccaatgttg atattcgttt ccggatccag caattgccca gggctgctat
    16441 aaccaggaat agagaacatc ttcaccgtat gggtcgctgt accaggcata atctgcatca
    16501 ggccgctggc ccctaccggt gatttcactt tcggattcca ggcgctctcc tgacgggcaa
    16561 tcgccatcgc atagctttgc gggatctctt tcccactggt gtaacgtttg aaaagatcgt
    16621 tgtaagccag cgggaatcgc tcttccagat gatcccaaag cttcccggcg atcgttgcct
    16681 gaacgctaag atcccaccat tggttattga aagcataccg agccagttga gcctgctctg
    16741 tttttgactt gctcttcacc agattggccc actcgctacg cgcggtgtta tcgagattcc
    16801 agtacatcaa ctcgcgcacg cgcgccatct ccggcccctg agtcagggcg ctgtcaacat
    16861 tctgcggcgc tttatcaatc ttcagctcat actcttctcc gatgcgttgt gctgcaacca
    16921 tcgggtagaa accacgctgt tgcatgagtt gatgcaaaat ctctttcgct tcagcttcac
    16981 gtccgcgttc cagcaataaa tccacctgcc agtaacgcca ttcatctttc tcttttgctt
    17041 ccatcggcag acgcgccagc caggtattca ggccgcgacg atcgccggtg ccaagtgcca
    17101 ttcgcacacg gcgttcaatc aacgacgtgg attgcgagcg cataatgacg tcatcgcgcc
    17161 atttcgcctg ctcgtcggtg acatcgttgc ccatcaaacg ccaggcgacg atatcgcgca
    17221 gctcctgaat ctgatcttca ttaagctgct gcacctgggc aagcgacggg atcatcagcc
    17281 gtgcattttc agcatcctgc cgcgccacac tggcaaacgc caccgctgcc atttgacggg
    17341 taaaatcggt cgctccggtt gtacgcgcga aggtcagtac cgtattaggg ttgttcgcca
    17401 gtgaaatgat tgccgaggcg atagtctggt aatcggcagg catctgccct gccagcactg
    17461 ttaccaggcc agtgttaccc gctttcatcg ccagacggat acgctctaaa tacgccagcg
    17521 gatcttgttt acctgacgca cgccagacgc taaataactt gtcacaggcg ttaggctggc
    17581 tcttgccggt tagccacagc tctttcgccc cttgccaggc ttcttcactc tgcccggtgt
    17641 tccatttcgc atagtagtaa ttacattgcg cttcggtagt tccgggcttt tccgggctaa
    17701 aggctaacaa gccacgccag tcttcacgcc gcgccagttc attgacgaaa cgagattgca
    17761 gcgtgcgagc gggaggaagc gtggggttag cgcgaacaaa gttagtgacc gtcaccgccg
    17821 gttgattcat cagatcatcg gtgatctggc ggtattccag gtagggataa agcggataat
    17881 ccttcagtcc aggcatcatt tgttcgacca catccatttg tcgattatcc caggcctgct
    17941 tgatttgcgc gtaacggcta cgctgctcat ccagtgagtc ggctcgcgcc acgctgctga
    18001 ccgtcagcag acagacaccg gcagccaaca gccgccaggt aacttgtttg gctttttcca
    18061 caagcgcatc ctctaagtgt aaatacatca atgcagcgtc atgccgcgta atagatttat
    18121 gctaaccagt cattgccgtt tacgccacgt tacggacact tttttacttt tactgcgaag
    18181 gtgatcgacc aactgggatt aggcggggaa aaaggctaca cttcgccttt gaaaactcat
    18241 catcacgata aacatagagg cgaagtccaa cgtggctcaa ttcgtttata ccatgcatcg
    18301 tgtcggcaaa gttgttccgc cgaaacgtca tattttgaaa aacatctctc tgagtttctt
    18361 ccctggggca aaaattggtg tcctgggtct gaacggcgcg ggtaagtcta ccctgctgcg
    18421 cattatggcg ggcattgata aagacatcga aggtgaagcg cgtccgcagc cagacatcaa
    18481 gattggttac ctgccgcagg aaccgcagct gaacccggaa cacaccgtgc gtgagtccat
    18541 tgaagaagcg gtttctgaag tggttaacgc cctgaaacgc ctggatgaag tgtatgcgct
    18601 gtacgccgat ccggatgccg attttgacaa gctggccgct gaacaaggcc gtctggaaga
    18661 gatcattcag gctcacgacg gtcataacct gaacgtacag ctggagcgtg cggcggatgc
    18721 gctacgtctg ccggactggg acgcgaaaat cgctaacctc tccggtggtg agcgtcgtcg
    18781 cgtagcgttg tgccgcctgc tgctggaaaa accagacatg ctgctgctcg acgaaccgac
    18841 caaccacctg gatgccgaat ccgtggcctg gctggaacgc ttcctgcacg acttcgaggg
    18901 caccgtggtg gcgattaccc acgaccgtta cttcctcgat aacgttgcag gctggatcct
    18961 cgaacttgac cgcggtgaag gtattccgtg ggaaggcaac tactcctcct ggctggagca
    19021 gaaagatcag cgcctggcgc aggaagcttc acaagaagcg gcgcgtcgta agtcgatcga
    19081 gaaagagctg gagtgggtac gtcagggaac taaaggccgc cagtcgaaag gtaaagcacg
    19141 tctggcacgc tttgaagagc tgaacagcac cgaatatcag aaacgtaacg aaaccaacga
    19201 actgtttatt ccacctggac cgcgtctggg cgataaagtg ctggaagtca gcaacctgcg
    19261 taaatcctac ggtgatcgcc tgctgattga tgacctgagc ttctcgatcc cgaaaggggc
    19321 aatcgtcggg atcatcggtc cgaacggcgc gggtaaatcg accctgttcc gtatgatctc
    19381 tggtcaggaa cagccggaca gcggcaccat cactttaggt gaaacggtga aactggcgtc
    19441 ggttgatcag ttccgtgact caatggataa cagcaaaacc gtttgggaag aagtttccgg
    19501 cgggctggat attatgaaga tcggcaacac cgagatgcca agccgcgcct acgttggccg
    19561 ctttaacttt aaaggggttg atcagggtaa acgcgttggt gaactttccg gtggtgagcg
    19621 cggtcgtctg catctggcga agctgctgca ggttggcggc aacatgctgc tgctcgacga
    19681 accaaccaac gacctggata tcgaaaccct gcgcgcgctg gaaaacgccc tgctggagtt
    19741 cccgggctgt gcgatggtta tctcgcacga ccgttggttc ctcgaccgta tcgccacgca
    19801 catcctggac taccaggatg aaggtaaagt tgagttcttc gaaggtaact ttactgagta
    19861 cgaagagtac aagaaacgca cgctgggcgc agacgcactg gagccgaagc gtatcaagta
    19921 caagcgtatt gcgaagtaat gcgtaaaatg ccggatgcgg cgtgaacgcc ttatccggcc
    19981 tacaaaccaa gaaatttcaa tacgttgtga atctccaggc ctgataagac gcggtaagcg
    20041 tcgcatcagg cattgagcac cgaatgccgg atacggcgtg aacgcctttt ccggcctaca
    20101 acagccttag tatccgcaat ttcgtagcgg gtcacccgtg caggtttttt caaaccttgt
    20161 ttaatctcga cagcctcttc cagagaggtt ttcaactcat caaaaaactt attttatacc
    20221 tcgcctttga gccgtttcat cgcggttatc tctgctcccc catcatctcc cgcaccagct
    20281 ccacgcagcg caggaaacgg ctgtcataat cgtcctcttc aacccgcacg aattcgatat
    20341 tgttctcttc cagcatctcc accagcaagt tctggaactc tttgcgatcc accgaactgc
    20401 cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt ctccagcagg atcaccagat
    20461 cgaaacggta ttcatcaatc aacgcctgta cgaacggatg ctcacgccct tcgtactttt
    20521 tgcagaacgc ctgggtagtg acaaaatcag tgtcaataaa cgccacttta ttggcatatt
    20581 tcactgcaaa atcaatgtat tgtgcgtggc ccagcgcgat tttatcgtaa tcggaatact
    20641 gcaatgcgat ctcatcaccg ccgaggtgtg aaaagacata atcgcgacca tattcccacg
    20701 cactggtggt attgaagata ttggcaagtt tgtttaccag ggtggattta ccgctcgact
    20761 cgccaccaag gatcgccacg gtacgtacaa agaacggctt cacttccgta ggaatatatt
    20821 cccagtagcg gaacgggttt tcgcggatct gcgcaccgct gatactcata aaggtacgtt
    20881 tcggatcgac cagcaccgta tcgatcccca gatgttccat atactgtggc gcatcggctt
    20941 cttccgaggt gtagatcagg tccggctgaa tccccttttc agccataaac tttttgatgc
    21001 cgttgctcca cacatcccag ccgtgcggat acggctccat gccctcttcg ttgaaagcat
    21061 gaatgcgaat atttttctga tatttaaaag tttgtaataa ccagcgcaga cgatccggca
    21121 ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg gtatcgtcaa
    21181 aacccataat gatatgcagc tcgtcaacct ggctacaggc gcgctggata aggtagatat
    21241 gtccggtatg cagcgggtag aacttaccga atacgacacc aatggttttc ttctgtcggg
    21301 gaaattcaag ccccaaaaaa cggtgcaacg cctccagctt ttgcgcgctg gggcttttaa
    21361 ttttggcatt cagtaactgg cttaaatacc ctttggtcat accgctggca tcagctacct
    21421 gctgtagcgt gcagccctgt tgcttgatgg cagttttcag gtaatcaaat gacgacatat
    21481 ctccctccgt atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg agagtttttt
    21541 aacgccaaaa atctgcatcc cttccggcgc ttttttcggt acgttagccg ccggaacaat
    21601 cgcccggcga aaaccgtgtt tcgccgcttc tgaaattcgt tcctgaccgc tgggcaccgg
    21661 gcggatctcc cctgccagcc cgacttcacc aaacaccacc agatcctgtg gcagcggtct
    21721 gtcacgcagg ctggaaacca tcgccagcag taacgctaaa tcggcactgg tttcggttac
    21781 cttcacgccg ccgaccacgt tcacaaatac atcctgatcg gccatttgca gaccaccgtg
    21841 acggtgcaag acagccagca ggattgccag acggttttgt tccagcccca ctgccacgcg
    21901 gcgcggattc gccatcatcg agtgatcgac cagcgcctga atctccacca gcaacggacg
    21961 cgttccttcc cacaccacca tcacggagct gccggaggtc acttcatctc cgcgacttaa
    22021 gaaaattgcc gaagggttgc tgacttcacg cagcccctgt tcggtcatcg cgaagacgcc
    22081 cagctcattc accgcgccga agcggttttt atggctgcgc aaggtgcgaa aacgggagtc
    22141 ggcatcacca tccagaagca ccgaacagtc gatacagtgt tccagcactt tagggccagc
    22201 cagcgagcca tctttggtta cgtgcccaac catgacaatc gccacaccgc gcgttttggc
    22261 gaagcgcgtc agataagccg ccgtttcacg cacctgcgcc acgctaccag gcgatgattg
    22321 tacatccgcc atatgcatca cctggatcga gtcaattacc atcagtttcg gttgctcttc
    22381 ttcggcaatc aggcagatct gttcgatgct ggtttccgac aacatattga gattgtcagt
    22441 cggcaggcca aggcgatgag cgcgcattgc cacctgttgc agcgactctt cgccagtgac
    22501 atacagcgtt ttcatctgct gggccagttt gcacagcgtt tgcagtagca gcgtggattt
    22561 ccccgcacct gggttaccgc caatcagaat ggcgcttccc ggcaccacgc cgccgcctag
    22621 tacgcggtcg aactctttaa atccggtgga aaaacgcggc agctcttcaa ggctgatatc
    22681 ggagagtttc tggacttttg ccaccccggc gctaccggca tagccgctga gacgctcgtt
    22741 acgcgccacc gttggcgacg cagcaagacg cacctcggtg atggtgttcc aggcatgaca
    22801 ggcactgcac tgcccctgcc agcgcggata atcggccccg cattcattac aaacaaaggc
    22861 gcgttttgga gcttttgcca cggtgtacct cgttaatgct gtgccgcccg caggatggcg
    22921 ggcgagcaat tacttctgat tcaggctgcc agagaggatg cagaataccc ccatcaggtc
    22981 agcgtgacgg atggtgactt ccgccttttc attcactttt ggcttggcat ggtaggcaat
    23041 ccccagccct gctgctttga tcatcggcag gtcattagct ccatcgccaa tcgccacggt
    23101 ctgcgccagc gggatttcat actcctgcgc gaggcgagtc aaagttttcg ctttgtactg
    23161 cgcgtctacg atgtcgccaa tcacattgcc ggtaaattta ccgtccatga tctccagttc
    23221 attggctacc acggcggtca ggcgcagctt gtcgcgcaga tattcagcaa agaaagtaaa
    23281 gccgccggag gcaatcgcca ctttccagcc cagcgtttcc agcttgagca ccagttgcgt
    23341 taagcctggc atcagcggca gattttcacg cacctgttgc agaatattgg cgtcagcgcc
    23401 tttcagcgtc gccacacggc tgcgcaggct ggcggtaaaa tcgagttcgc cgcgcatcgc
    23461 ccgttcggtt acttccgcca ccatctcgcc cgttccggcc agtttggcaa tttcatcaat
    23521 acattcaatc tggatggcgg tggaatccat gtccatcacc agcaaacccg gcgtgcgcag
    23581 gtgcgggatt ttccccagcg gggcgacatc cagttgcgct tcatgggcca ggcgcgtagc
    23641 ccgtgcggtg agtgaacctg ccagacgaat cacctgataa tcttccacgc accaggcggc
    23701 aacaatcacc atcgccgcac ccagtttgct ctggtattgg gtcagacgct gtttatccag
    23761 cccacgacca tacagcagcc agccgctacg acctgcgtgg taatccagtg gcatcacttc
    23821 atcaccactt aatgaaagag gcagacccgg ccataaagag acatcttcag gcaggtcgca
    23881 ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc gaagcaggga aaataacgca
    23941 tgaggctacc ttgtatccat tgcttctggc aacattaagt ctcaaatttt caaagggtgg
    24001 aagatggctc gcacaaaact gaaattccgg ctgcatcggg cagtgattgt cctgttctgt
    24061 cttgccttgt tagtggcact gatgcagggg gcgtcatggt ttagtcaaaa ccaccagcga
    24121 cagcgtaatc cacagctgga agaactggcc cgcaccctgg cgcgtcaggt gacgctgaac
    24181 gttgcaccgc tgatgcgtac cgactcacca gatgaaaaac gcattcaggc gatcctcgat
    24241 cagttaacgg atgaaagccg catcctcgac gcgggtgtgt atgacgaaca aggcgatctt
    24301 atcgcccgtt caggcgaaag cgtcgaagtg cgcgaccggc tggcgctcga cggtaaaaaa
    24361 gcaggcggct attttaacca gcagattgtc gagccaattg cgggtaaaaa cggaccgctc
    24421 ggctatctgc gcctgacact cgacacccat acgctcgcca ccgaagccca acaggtggat
    24481 aacaccacta acattttacg cctgatgttg ctgctctcac tggcaatcgg cgtagtgctg
    24541 acccgcacgc tgctacaggg taaacgcacc cgctggcagc aatcgccctt cctgttaacc
    24601 gccagcaaac cggtgccgga agaggaagaa agcgagaaaa aagagtgacc cattactaca
    24661 agaaaggaaa tcgttatgtc cacattacgc ctgctcatct ctgactctta cgacccgtgg
    24721 tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca gcgcgttctg
    24781 tttctctggc gcaatgccga cacggtagtg attggtcgcg cgcagaaccc gtggaaagag
    24841 tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag tagcggtggc
    24901 ggcgcggtgt tccacgatct cggcaatacc tgctttacct ttatggctgg caagccggag
    24961 tacgataaaa ccatctctac gtcgattgtg ctcaatgcgc tgaacgcgct cggcgtcagc
    25021 gccgaagcgt ccggacgtaa cgatctggtg gtgaaaacag tcgaaggcga ccgcaaagtc
    25081 tcaggctcgg cctatcgcga aaccaaagat cgtggcttcc accacggcac tttgctgctc
    25141 aatgccgacc ttagccgcct ggcaaactat ctcaatccgg ataaaaagaa actggcggcg
    25201 aaaggcatta cctcagtgcg ttcccgcgtg accaacctca ccgagctgct gccggggatc
    25261 acccatgagc aggtttgcga ggccataacc aaggcctttt tcgcccatta tggcgagcgt
    25321 gtagaagcgg aaatcatctc cccggacaaa acgccagact tgccaaactt cgccgaaacc
    25381 tttgcccgtc agagtagctg ggaatggaac ttcggtcagg ctccggcatt ctcgcatctg
    25441 ctggatgaac gctttagctg gggcggcgtg gaactgcatt tcgacgttga aaaaggccat
    25501 atcacccgcg cccaggtgtt taccgacagc ctcaaccccg cgccgctgga agccctcgcc
    25561 gggcgactgc aaggctgcct gtaccgcgcg gatatgctgc aacaagagtg cgaagcgctg
    25621 ttggttgact tcccggacca ggaaaaagag ctacgggagt tgtcgacgtg gatagcgggg
    25681 gcggtaaggt aactcgctga ttaaaatacc aaaggaaggg aatatgatcg atctggaaaa
    25741 ccaggagcgg gagattatca atttaatgtt aagccagcgt atttcctggc tagccgccgt
    25801 gcgtatcagg cataaattat cacttgctga agtaagtaaa atgttaggta tctcgattaa
    25861 ttctctaaaa tagatcgaaa agacggaacg gcttagctca aacatcaaaa gcaaaatggg
    25921 agaaatttat ggttgtccac cagagttgct gatatgccct tcctggatga cggctgaaca
    25981 taaatgaata gattcgtgaa tagatacttg aatgtatttt attttttatc catatgaaaa
    26041 ataatagtaa atctataaat cagttgctat tgatgaatag atactaaaaa gccggagcag
    26101 tcccccactc cggcttaaca cgacaatcgc ctttcagcga aacacaatta ctctttatcg
    26161 cccagcagaa cggattccag tgcgattttg atcatgtcgt tgaaggtggt ctgacgctca
    26221 gcggcagtgg tctgctcgtg agtgcggatg tggtcagaca cagtgcagat ggtcagggct
    26281 ttcgcgccaa attctgcagc gacgccgtag ataccagccg cttccatttc cacgccgagg
    26341 atgccgtatt tttccatcac gtcgaacatt tcgccgtccg gagagtagaa caggtcagcg
    26401 gagaacaggt taccaacgcg agcatcaatg cccagtgctt tagccgcgtc taccgcgtta
    26461 cgcaccatgt caaagtcagc gatagcggca aagtcatgat ctttaaaacg gatgcggtta
    26521 actttggaat cggtgcaggc acccataccg ataacgacgt cgcgcagttt tacgtgcggc
    26581 agaactgcgc cacaggaacc cacgcggata attttcttca cgccgaaatc ggtgatcagt
    26641 tctttggtgt agatggagca ggacgggata cccataccgt gacccattac ggaaattttg
    26701 cggcctttgt aagtaccggt gaagcccagc ataccgcgaa cgttgttcac ttcacgggca
    26761 tcttcaagga aagtttcagc aatatacttc gcacgcagcg ggtcgcctgg catcaaaact
    26821 acgtcagcga aatcgcccat ttctgcatta atgtgtgggg tagccattgt tttatcctta
    26881 tcaacatgtt ttcaccctct ccctgaggga gagggccggg gtgagggcat caagccgcac
    26941 ccattatcag aacatggctt tgccatattc catatcagaa gtaccaaaat attttgccag
    27001 agtctggccg atatccgcga aggtttcacg gtgacccagt gagcccggtt ttactttcgg
    27061 gccgtaaacc agtaccggaa tgtgttcacg cgtgtggtca gtaccggtcc aggtcggatc
    27121 acaaccgtgg tcagcagtga ggatcaggat gtcgtcatcg cgcagcagag acatcagctc
    27181 cggcagacgg cggtcgaaca gctccagacc cacagcataa ccggcgacgt cgcgacggtg
    27241 gccccaggaa gagtcgaagt caacgaagtt ggtgaagacg atggtgttat cacccgcttc
    27301 tttcatctct ttgatggtgg cgtcaaacag cgcgtccagg ccagttgctt tcactttctt
    27361 ggtgataccg cagttggcgt agatgtctgc aattttaccg acagaaacca cctgaccgtg
    27421 tttttcatca accagtttct gcagcacggt cggtgctggc ggctcaacag ccaggtcgtg
    27481 acggttaccg gtacgctgga agttaccggc tttgtcaccg ataaacggac gagcgataac
    27541 acgaccgatg ttgtagccgc cgttggtcag ctcttcacgg gcgatttcgc acagttcgta
    27601 gagtttatcc aggccaaagg tttcttcgtg gcaggcaatc tggaacacag agtcagcgga
    27661 ggtatagaaa atcggcttgc cggttttcat gtgctcttcg cccagctgat ccagaatgac
    27721 cgtaccggaa gagtggcagt taccgaggta acccggcaga ttagcgcgtt caaccagttt
    27781 atccagcagc tcttgcggga agctgttttc gtgatcggag aaatatcccc actcaaatag
    27841 aaccgggaca ccggctattt cccagtgacc agacggggta tctttaccgg atgacatttc
    27901 gtgcgcccat gcgtacgcgc cgataacttc agcgttgccg tccattcccg ccggaatgaa
    27961 accggtagaa ccttcgtgtg ctttcgccag ccccagacgg gtcagatttg gcagattgag
    28021 cggaccttta cgaccgttat cagcttcgcc tttggcacaa gcttctgcga tatgacccag
    28081 ggtgtcagcc ccgacgtcac caaagcgttc tgcatcttct gtagcgccga tgccgaatga
    28141 gtccagcacc ataataaatg cacgtttcat atgttctccg tactcagtgc ttcaaattta
    28201 aatgatcaga tcagtatacc gttattcgct gatacggcga tagacagttg gtgtgctttc
    28261 cggtgcttta tcggcaagtt taattgccgc tttcaccgct ttcgccgctt cctgccagct
    28321 gttttcgtct ttcgcgtgga taaccgccag cggacgctga ccgtctacct ggtcgcccaa
    28381 acgcgccata tcagtaaagc cgacgctgta atcaatggtg tcagatgcct gacgacgacc
    28441 gccgcccatt gcaaccactg ccatccccag cgcgcgggta tccatttcac tgacaaaacc
    28501 ttcggtatca gcatagactg ctttcgtcag catcgctgtc ggcagatact tcgcgtagtt
    28561 ctcaacgaag tcggttgggc ctttttgtgc cgctaccata cgaccaaaga cttctgccgc
    28621 tttaccgttg tccagcactg cttgcaattt cgcgcgcgct tcggcgtcat ctttcgccag
    28681 tttgccggag ataagcatct ccacgcacag cgccatcgtg acatcaaaca gacgcgggtt
    28741 acgatactca cccgtcagga actgcaccgc ttcacgaact tcaaccgcgt tacctgcact
    28801 ggaggccagt acctgattca tgtcggtgag cagcgcggtg gtgcgcacgc cagcgccgtt
    28861 agccacgcca acaatcgctt cggcaagggc ttcagagagt tcgtaggtcg gcataaacgc
    28921 gccgctaccc actttcacgt ccatcaccag cgcgtccaga ccttccgcaa gtttcttcgc
    28981 cagaatagag gcggtgatca gcgggatgga gtccacggtt gcggtaatat cacgggtcgc
    29041 gtagaaacgt ttatccgccg gagccagtga gctggtctga ccgataatcg ccacgccgac
    29101 gtctttaata atttcgcgga aacggttgtc atccgggaaa atgtcgaagc cagggatgga
    29161 ttccagtttg tcgagcgtac cgccagtatg accgaggccg cgaccagaga tcatcggaat
    29221 atagccgccg caggctgcga ccatcggccc caacatcagc gaagtcacat cgccgacgcc
    29281 accagtggaa tgtttatcaa caatcgggcc attcagatgc aggcttttcc agtcgagaac
    29341 ggttcctgaa tctcgcatcg ccatggtcag cgagacacgc tcaggcattg tcatatcgtg
    29401 gaagaaaatg gtcatcgcga gggcggcaat ctgcccttcg gagatagtgt tgtcgcgaat
    29461 gccgttgata aagaaacgaa tttcttcatc gctcagtgca tgaccatcac gttttttacg
    29521 aataatttct tgtgcgagaa acaaggtaac ctcccgagga aaagagtatg aggcggcttt
    29581 tgccggatgc gtctacgcct tatccggcct acgggtagat acgtctcgta ggcctgataa
    29641 gcgcagcgca tcaggcgtaa agcatcttac ttagtagctg ctggcgctct taccgtcacc
    29701 gtggcccagc gctttcaaca ggcttgccag caggctggaa gcaccaaagc ggtagtgacg
    29761 cgcatctgcc cagtcagcac cgaacagttc atctgcgatg gcgagatatt tctgcgcatc
    29821 ttccgcagta cgcacgccgc ccgctggttt gaaaccaacg gttttttcta cgcccatatc
    29881 acggatcact tccatcatga tgcgcgcgct ttccggcgtc gcgttcacag ccactttacc
    29941 ggtagaggtt ttgatgaagt ccgcacccgc tttgatggag atttcagacg ctttacggat
    30001 cagcgcttcg tctttcagtt cgccggtttc gatgatcact ttcagcagta cattcgctgc
    30061 cgcgcaagcc tctttacagg ctttcaccag gtcaaaacca acctgctcgt tacccgccat
    30121 cagcgcgcgg tacgggaaca caacgtcaac ttcatcagca ccgtaggcga ttgccgcacg
    30181 ggtttctgcc agcgcgattt cgatgtcatc gttaccgtgt gggaagttgg ttaccgtagc
    30241 gattcggatt tccggggtgc cctgctcttt cagcgttttg cgagcaatcg ggataaagcg
    30301 aggatagata cagatagcgg cggtattgcc gaccggggtt ttggcctgat gacacagagc
    30361 aattactttc tcgtcggtgt cgtcgtcatt cagggtggtc aggtccatca atttcagtgc
    30421 acgcaggctg cttgctttca gatcagtcat ttcattctcc tggcttgtcg ccaataaaat
    30481 tcaccttgcg agtttgttag tattctaaca tctactccgc aacacacttc gatatacatc
    30541 acaattaagg aaatctactt acaagtttgc atcactgtaa tgcgatctgg ttcaaataat
    30601 tcactttcaa atgaatgcgt cagtggtggc aaacgcatca ggatcaaagt gaacatcacg
    30661 aaacttctta caatggcgca tcaccttcgg cataaaagga aaagaagatg cccacttctc
    30721 atgaaaatgc actgcaacaa cgttgccagc aaattgtcac cagcccagtg cttagcccgg
    30781 agcagaagcg ccattttctg gcactggaag cagaaaacaa tctgccttat ccgcagcttc
    30841 ctgccgaagc ccgccgcgcg ctggatgagg gtgtaatctg cgatatgttt gaaggtcatg
    30901 cgccgtacaa accgcgctat gtcttacccg attacgcccg ttttctggcg aacggttccg
    30961 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
    31021 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctggggcaa ctggatgcgt
    31081 tgttgcaacc atatgttaga attctaacac aagacgagat cgatgttcga ataaaacgtt
    31141 tctggcgtta cctcgacaga accctgccag acgcctttat gcacgccaat atcggcccgt
    31201 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc agagttgaag caggtttcac
    31261 cgaacctgac ctttatctac gatcctgaaa tcacccctga tgacctgctg ctggaagtgg
    31321 cgaagaacat ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa
    31381 ttttcacaaa agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg
    31441 gtggcagcac gctggtacgc cttaacctga aagccattgc cgagcgcagc gaatcgctgg
    31501 atgacttctt tacgcgcact ctaccgcact actgccagca gcagatcgcc atcatcgatg
    31561 cgcggtgtga attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag
    31621 aagggctgat taaccctgaa cgttttgtgc caatgtttgg catgtatggg ctggcggaag
    31681 cggttaactt gctgtgtaag aaagaaggga ttgccgcgcg ctacggtaaa gaagccgccg
    31741 caaatgaagt gggttatcgc atcagcgcgc aactggcgga gtttgtcgcc aatacccccg
    31801 tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca gtcggggagc agttccgata
    31861 tcggcaccac gccgggcgcg cgtttgccgt atggtgatga gccagatccg atcacccatc
    31921 tgcaaactgt cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc
    31981 tcgacgaaac catcaaacgt aatccgcagg cgctggtaca gctttgcctc ggtgccttta
    32041 aagccggaat gcgtgaattt actgccaatg tcagcggtaa cgatctggtt cgcgttaccg
    32101 gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca
    32161 ccacctggct gggcgaagaa gccgcacgca acactcgtat tctggaacgc cagccgcgcg
    32221 tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt
    32281 gacgggccag gcagtcgtct ggctctgttt ttgcagggct gcaatctgcg ctgcaaaaac
    32341 tgtcacaatc cgtggacgat gggacgttgc aatgactgtg gggagtgcgt gccacagtgt
    32401 ccgcatcagg cgttgcagat tgttgacggc aaagtggtgt ggaacgctgt ggtttgcgag
    32461 cagtgtgata cctgcctgaa gaggtgtccg caacatgcca cgccaatggc gcaatccatg
    32521 agcgtggacg aagtgcttag ccatgtccgc aaagcggtgc tgtttattga agggataacg
    32581 gtaagcggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc
    32641 aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg acagtaacgg catgttgagc
    32701 gaaaccggct gggaaaaatt actcccggtg tgtgacggcg caatgctcga tctcaaagcg
    32761 tgggggagcg aatgtcatca acacttaacc ggacgcgata atcagcagat taagcgcagc
    32821 atctgtttgc ttgcagagcg cggcaagctg gcggaactgc gtttgctggt aattccagac
    32881 caggtggatt atttgcatca catcgatgag ctggcgacgt ttatcaagag acttggcgat
    32941 gttccggttc gcctgaatgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg
    33001 gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttaaaggt gcgcggggtg
    33061 agccggttga tatttccggc gctctatttg tgacatgccg gatacggcct acggttcggc
    33121 acagacttgt aggcatgata agacgcgaca agcgtcgcat caggcattat gcgccgactg
    33181 ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacagacttg taggcatgat
    33241 aagacgcgtc agcgtcgcat caggcattgt gcgccaactg ccggatgcgg cgtgaacgcc
    33301 ttatccggcc tacggttcgg cacaaaccag taggcatgat aagacgcgtc aagcgtcgca
    33361 tcaggcattg tgcgccaact gccggatgcg gcgtgaacgc cttatccggc ctacggttcg
    33421 gcacaaacct gtaggcatga taagacgcgt taagcgtcgc atcaggcatt atgcgccgac
    33481 tgccggatgc tgcctacgaa gggtaggcta cccactaacg ctaaacaacg catacgtatt
    33541 attaagcaac acttcggcaa tctcatcttc tggttccggg cgcaactcgc aaagcacgtc
    33601 aaacacccgc acagcctgtt ccggtcggtt aggctgcccc tgaaagccgt tgagcggcat
    33661 atccggcgcg tcggtttcca gcaacaacga tgccagcggt aattttgcga tgacatcgcg
    33721 ggttttactg gcgcgtggat aggtgatagt accgcctacg ccgattttgt agcccagctg
    33781 tacaaaccgc tcggcctgtt gcaggctgcc ggaaaaaccg tgaaccacgc cagtgcacgg
    33841 taaatcgtga cgtttaagat gcatcgccag tttgtcgtgt atacgccggg aatgaaggat
    33901 caccggcaga tcgtagcgtt tcgccagttt cagctgttcg tcgagtaacc actgctgcct
    33961 ctcaaattgc ggatcgtcgc cgaagagatc cagaccgatc tcccccaccg ccaccacctt
    34021 cgccggacgc ctttccagcg cctgctgtag ctgatcaaga gacacatcgc tatgtttttc
    34081 caacatcccc ggatgcaacc ccaatgcggc atacagcggt tgatattttt ccgctaacgc
    34141 ctgcacgcgg gcaaaatttt ccgcctctgt cgccggaaca atgatcttgc ctacgcccgc
    34201 ttgtgccgcg cgttgcaggc tggcctcttc atcgccacta aacggcggga aatcaaaatg
    34261 gcagtgggta tcgataaaac ggcaaatcaa gccagatcct cattgttaaa tgtcgggtcg
    34321 ttcgcctgcg gagcatcgct cacttcggca accagcgtat cgttggcgac tggcgcaggc
    34381 ggaataacga tcgattccgg cgtcaccact ggcaccagat ggcgggtaag cggcgctttt
    34441 tcagttaata gcttgccaac cgtggcgagg aaataacgac cgcataaacg cccaagttta
    34501 tagtcttcac gcagcgccgg aatccgactg ccaagcgcga tactatgtaa tggcttcggc
    34561 ggataaattt cgaatatccg cagcttgccc ggtggtttct caataaattg ctgaatgtca
    34621 cgatagctgg tttcatgatg ctgcaccaga ttgaccagcg gctgcaggct actgtcaccc
    34681 agccagcgtt ccatgcgttt gaaccactgc ggcgtgtagt acatttgtga cggcacagtg
    34741 cgaatgacga ccaacgtttt agcgccctgc cttgccgcct ctttaaccgg aatcgcatca
    34801 ctgatcccgc catccaggta gttaatgcct tccagcgaca ctccgctacg ataaaagcca
    34861 ggtatcgcac tggaggcgcg aatcacatcc agccagtttt gtttggttgg taaaaagtaa
    34921 ttaggcgcgt agtcatcctg acgacaggcg cacatataaa acgatttgcc gctgtcaaac
    34981 aaccgcgcgg cggtatccat ttgcaacggc atctggcttg cggtggcctc caccagccag
    35041 tcgagatcga taagatttcc tccacggaca aagcgcaatg gatcgaaaaa ttcgcgtttt
    35101 gtggtatagc gcatgatgac tttgcgcgcg taaccgggct gattgcagat aaacgccgag
    35161 aggttctgcg ccccggcaga tgtgccgaga taaagatcga aaggattaaa ctgcgcgcgc
    35221 ataaactcat ccagcacgcc agccgtgaaa attccacgct gtccgccgcc ttcgcacacc
    35281 agagctattc gtccaggctg gaacggcctt agcgacaacg gcgcaatatt accaagcgtt
    35341 acaggtattc gctgccccac ctctgccttc ctgtttttgt tgtgaaagaa tgacaaagta
    35401 acgcaattta ttattcgcta aaaccgcaaa agccagtccg ctggactggc tttggataaa
    35461 attttcttaa tggtctgaaa tctagggtcg ttttcggccc atgaacaaac tcaccaggaa
    35521 cagaataatc ccgacgacaa agacaatttt agctgcgcct gcagcggtac cggccagacc
    35581 accaaaccca agtgcggcgg cgattaacgc gataaccaga aatatgatgc cccaacgaaa
    35641 cataagcgtc tcctttacca tagttaatgt caccgcttga tatgagcgtg aaaatcactc
    35701 aatgcgatat ttttagtgtg gtgcacatcg cgcctcccga caaagttcgg gaggacgaat
    35761 tacgacgaat tacttagttt tcagatcatt tttaacgctt ttcacaccat ctaccgcttt
    35821 ggcgatactt tcagcacggt cgctctgtgc ctgggaatcg acggtaccgg agagctgaac
    35881 cacgccgtcg gtggtttcaa ctttcacatg acgggaaggg acgatatcgt ccgccagcag
    35941 tttggctttg atttcactgg tggtggcggt gtcacccgcg tagcccttca cagagccttc
    36001 tttagcgtcg cgaacgtgca gtttgtcgct gacagaggtc accccttcaa cgcctttcgc
    36061 cactttcact gcctcttcgg cctgagcctg gctttcaacg aaaccgctca gggtcacgac
    36121 tttttgatcg gttttgacag agatatcggt gctcttgatg ttgtcatgat ccaccagcgc
    36181 cgccttcact ttcgcggtga tggcgctgtc atccatgaaa ttaccgactt tattcataga
    36241 gctatcgact ttttgccctg cgctttcatt ggtagtctgt gcgttgtttt ccgcgtaggc
    36301 agagccggtc gcgacggcag aggtcaacat tacagccagc agagttttcg aaatcttcag
    36361 tcttgtcata gtcatcgatt tattcctgta tgtttgctcg taatttgagc ctggcaacac
    36421 gaggttgcat tgctgaatag ggagagactt caccctctac agaagtcaat ggtcgccatc
    36481 acaaaagcga tgggtgatga ataacgacca ttacagcctc tgaatcagtt attaatatcg
    36541 gtagaatgac aatcgacggc tttagatact gatatctacg cattgaacgg tatttaacgc
    36601 cgtcagaaat gtcattactt tgttaaatat agatcacaat tttgaaaccg ctcgggatat
    36661 cagcgaaaac ataagcaaaa gtgaatgttt taagaacatt ccgtaagcgg ctaataagga
    36721 agggaaattg acagggcgca gcggctaccg cgccctggag gcaagaatta atgctcgcgg
    36781 gtctggtgga actgaacgtc gggataacgt tcctgcgcca ggcgcaggtt gaccatgctg
    36841 gtagcgatgt aagcgaggtt atcgccgcca tcaagcgcca gttggctttc gttcttacgc
    36901 ttgaactctt cgaatttctt cgcgtccgca cattctaccc agcgggcagt ggcaacgttg
    36961 actgattcat atactgcttc aacgctgtat tcgctcttca ggcgcgatac caccacatca
    37021 aactgcagca caccaacagc accaacgatc aaatcgttgt tggagatcgg acggaacacc
    37081 tgcaccgcgc cctcttcgga aagctgtacc agccctttga gcagctgttt ttgcttcagc
    37141 ggatctttca ggcggatacg acggaacagt tctggtgcga agttcggaat accggtgaac
    37201 ttcatcatct caccctgggt aaaggtgtcg ccgatctgaa tagtgccgtg gttgtgcagg
    37261 ccgaggatat cgcccggata cgcttcttca acgtgcgaac ggtcgcccgc cataaaggtc
    37321 agcgcgtcgg agataaccac atctttcgca gtacgcacct ggcgcagctt catgcctttt
    37381 tcatatttac cggatactac gcgcataaac gccacgcggt cgcggtgttt cgggtccatg
    37441 ttggcctgaa ttttaaatac gaagccggta aatttgtctt cgctcgcctc tacggtacgg
    37501 gtatcagtct gacgcggcat cggcgcagga gcccactcca ccaggccatc cagcatatga
    37561 tcaacgccga agttacccag cgcagtaccg aagaataccg gagtgatttc gcccgcaagg
    37621 aacaattctt tgtcgaactc gttagacgcg cctttcacca gttccagttc gtcacgtagc
    37681 tgctgtgcca gatcttcacc aaccgcagcg tcgagatccg ggttattcag ccctttaaca
    37741 atacggactt cctgaatcgt gtggccttta ccgctctgat agagataggt ttcgtcttta
    37801 taaaggtggt aaacgccttt aaacagcttg ccgcagccaa tcggccaggt gatcggtgcg
    37861 cagccgattt tcagctcgtt ctcaacttca tcgagcagtt ccatcgggtc gcggatatca
    37921 cggtcaagtt tgttcataaa ggtgaggatc ggcgtgtcgc gcagacgggt aacttccatc
    37981 agcttacggg tacgatcttc aacacctttt gcagcgtcga taaccatcag gcagcagtcc
    38041 accgccgtca gggtacgata ggtatcttcc gagaagtctt cgtgccccgg ggtgtcgagc
    38101 aggttaacca ggcaatcgtg atacggaaac tgcatcacag acgtagtaat ggagatccca
    38161 cgctgctttt ccatctccat ccagtccgac ttagcgtgct ggttggaacc acggcctttt
    38221 actgtaccgg cggtctgaat ggcctgtccg aacagcagca ccttctcggt gatggtagtc
    38281 ttaccggcat ccgggtgaga aataattgca aaggttcttc ttttcgctac ctctgcggcc
    38341 aaaaggcttg tcataattgt tgtctcgttg attatgtaca atcatatgta caaagaaaaa
    38401 ttcatgccgc tgattgtaca ttttggaggt atcagagcat agcagaacat gatcagtagg
    38461 tgtatttgta gccaaagctc atttgtacat gccgcaatct tcgttctatt gacactgttt
    38521 attcacccag ttaaaaataa gactgtatac aaacacagta tagagggaat tttatgcgta
    38581 ttgagatctg catagccaaa gagaaaatga ctaaactgcc aaacagcgct gtggatgcgt
    38641 tagaaggaag aattaacccg acgcaccggt aagcgttatg aagatgtaga ggtgatcgta
    38701 aaagccacca gcaatgatga cctttctgtt acacgcaccg ccgataaaga ttcagttaaa
    38761 atttttgttc aggacactct gaaagatacc taggaatttg ctgacgagtg atttagttca
    38821 tcatctctgg acacaattca cttgtattga ctttaagatc attaaattgt aataattcaa
    38881 aaggatgggg cagttttttc ctcttgcgta tgcaagggcg gctctgataa tgaacagtaa
    38941 tattttactg ttgagcttat gctcactgat cctgccgagc aggatgttca acggttccaa
    39001 tatatcctgc cccttccgct ttttaaaagg atattttatg catgaaaata tttggtttac
    39061 atataaagcc cgtattcaag cgcaccatag actagaatgg cttgaaaaac actctcaatt
    39121 tatcctcgtt tggtatgcta tattgagtgc ggtactttca attataacgt tacgatttcc
    39181 aaaatttcta ggagataata cagatatcgt tgcggcaata ctttcagtgg ctctactggg
    39241 tatttctctg atcgtatcta acctagatta tcgtggtcga gcaatagcca tgagaaggaa
    39301 ttatattgca ctacagagac tctattttga tattaataac ggccaacagt taactcttga
    39361 acagaaagaa aaatatttta atttgctcaa tgaggttgag aatcatcgtg acatagatga
    39421 taaagtttca agggtaactc aagttggact tacgactaga ctccccacac aaaaagaaaa
    39481 aataattgtt aaattatgga tattacgtcg aatgtttatt actgtcggcc tttatatcct
    39541 cccattaata tgtctttgga ttgactatga atgcaagcag agtttttaaa aagtcattct
    39601 cgaaaaaaaa ccttctaaaa gtatacaatg aaaaaatcaa agaatcagga gcgattggca
    39661 tagatcggat tcgtccatca aaacttgatt taacaataaa gaatgagatc acttttattt
    39721 ttgaaaaggt taattgtgga aattataaat ttacagcata taaagaaaaa ttaatatcta
    39781 aaggggctaa ctctatcccc agacagattt ccataccaac tgccagggac agaattaccc
    39841 ttagggctct ctgtgaatgc cttacagaaa tatatcctaa ctccagatta aaactaccac
    39901 atacagtaat tgacttattg aaaaatgcat taaacagcga tctatatgct gaatatgcaa
    39961 aaatagatct caaaagtttc tatccttcaa ttgaacataa attgatattt aatgcaataa
    40021 aaaataagat tagaaaaaaa gaaattaggc agttaataac atcatcatta atcgtgccta
    40081 ctgtaagtgg aagtacagga agcaaaggtg tccctaataa taccagagga gtacctcagg
    40141 ggttagcaat atcaaacatt ttagctgaaa tatcactatc tgatttcgac aatgaaatca
    40201 ataaaatgca tgacatatgg tacatgcgat acgttgatga cattcttatt ttaacgcaaa
    40261 aagatcaagc aacaaaaatt gcatctcata tcattgataa gcttcaatca ttaaatttaa
    40321 acccacatcc attaaatgaa gagaactcaa aatccaaagt aggcagcttg gatgaaagtt
    40381 tcaacttttt gggctaccac ataaaaaatc gagaattatt gataaaacat gagagcattc
    40441 ttagatttga gtcatcctta gcaaaaattt ttactgcata tagacacgct ctactacaag
    40501 ctaaaaataa gcgcgataaa gaacgagctg ttgcatattg tcagtggaaa ctaaatctta
    40561 gaattacggg atgtgtgttt gaaggtaaac gattgggatg ggtatcgtac ttctcacaaa
    40621 taacgtcaac agctcaactt cgagctgtta atcatacgat caataatctt atccgccgat
    40681 tcggcctttc atcagaaata aaaccaaagt ctttgattaa aactttctat gaactccgca
    40741 ggggtagagc ggagactttt aaatacatac ctaactttga caatctacat atatctcaga
    40801 aacgagaaat tgtttctatg tggataggga aagagaatga aaaaaaactt agcaatagtg
    40861 aaatagagag aaaatttaaa tttaaaattg cgaaatcagt aaaagagctt gaagaggaca
    40921 tttcagggat atcatagata tgtaatccat taaatccttt atatataaag caataaacac
    40981 actatttaaa ttcacaaatc agccgcagta tcctgccatg acaagttgct gcggcatatc
    41041 acaaaacgat tactccataa cagggaccgc aggccactca atatcaggtg cagttgatgt
    41101 atcaacacgg ttcagcaaca cccgatactt tttccaggct tccagcaatg agttttcttc
    41161 ctccgttgcg atttccagat ctacagcatc ctgaagtggc gcaatatgct cactggctac
    41221 ctgcatcagg ttgttttttg tttcttccgc ctcccggatc cggaacagtt tttctgcttc
    41281 cgtatccttc aaccaggctg tgccgttcca cttctgatat tccccttccg gcgataacca
    41341 ggtaacattt tccggtaacg gaccgagttc agaaataaat aacgcgtcgc cggaagccac
    41401 gtcataaacc gttttacccc gatgatcttc aacgagatgc cacgatgact catcactgtt
    41461 gaaaacagcc acgaagccag ccggaatatc tggcggtgca atatcggtac tgtttgcagg
    41521 cagacctgta tgaggtggaa tatatgcgtc accgtcacca ataaattcat tagttccgga
    41581 caacagatta taaattttta tggtccgtgc ttgttcactc attctgaatg ccattatgca
    41641 agcctcacaa tatagttaaa tgcgatgttt ttgacggtgt tttccgcgtt accagcagcg
    41701 ttaacggtga tggtgtgtcc atgtgagcca atcgcaaccg agtgcgtatg cgcaccaata
    41761 cctacagtat gtgcatgtgc gccagaactt gctgcagtac cagacagcga gtgggtatga
    41821 gcacctgctg actgtgtctg aatacgttga taatacgatc tactggaaga agtccccggg
    41881 cttacttgat actgtgaatc atggacataa gtgaacccac cgccatcata aaatgctaac
    41941 gcagaaccgc cgcctcctgg ccaacgaata ccattaccat gagtatgagc accggcagac
    42001 cccgtagagc cactcagact gtgcgtatgc gccccggtgt tattcgtgga tttagtgccg
    42061 taatcaaacg acgatgtggt tttcgtcccc aaatccgtac tggatgcgct ggcgctgtgg
    42121 gtatgcgatt ttatgccgtc ctgttcctga gacaatacgg cacgaccact ggcgggcttg
    42181 cccttaatcg tccagccacg catatcaggg atcacgcctg acggataagc ggctgcaagt
    42241 ttcgggtagg cagatttgtc aaaagtctgc ccctgcatca gggcataacc agacggaacg
    42301 gtatctgatg gccacgggat tggtgcgccg actgggtagc tttctggtgg aagatttttc
    42361 gaggtataaa cttctgccca gtcttcctca aaaccataac cgtctcttga agaacggtag
    42421 aacagaccac catttctgta atgcgccttc atctgcaagg tccggcaact tccgactccg
    42481 gtatagaagt taaccagaat atagctgtcg ccagagcggg tgacattata agcgcctgat
    42541 tcggcattcc agggaacgcc accatccgca tcggcatatg tatccgttgc ccttctggca
    42601 aaagcagcca catgcgcggc ggttaaagta atatctttgg aaccatcaaa ctcaacacca
    42661 gaaacccgtc ttggcgtttg cagctttgtt gctgttaatg cattaccgtt cagacttgcg
    42721 gacagtttgg ttccaataac cagttcgccg gttgcgttat caatagcaaa cggtcttaat
    42781 gtattccagc caccataaac atcaccttga ttggtaagca gcaggtaagt tttagcgcca
    42841 tcattacgcc ataatgcccc atactcccca cctatcattc gaatctgatt accaccacgc
    42901 gctacaattt cgtctgtggc aaaaagtttt ttgcacgaca agttatcgtt aacgattaac
    42961 gaatgagact cataaaaacc acgcccactc ttaaaatcaa ggataacgtc cgccgcgata
    43021 cattcagtcg ccggatttgt tgccccaaac ttataggtcg tatcattaac aacgagatca
    43081 gcaccaggtg cggatattga caggccatct tcgataaacg caaaaacagg gaaagcagcg
    43141 ccatcaacat agaacacaga gcgcaaatca tcgcccttat tactcatcat tattgagtgg
    43201 atggctcgtt cattgttttg atattgccag aacattccat aagcataacg ccccctgtca
    43261 gtccagccac caggcataac aaatccgtta aactcgcagt tattcatcgg atcgcctgcg
    43321 gttcgcgttg ccgtggtgat aatgaccctt gatgccagtt cgcttactga gccagcagaa
    43381 cgcataacaa caacagggta atattttcca gatgttgcac ctgcaggagc gttaacccgc
    43441 acataacgca taccacgctt atcagcaaag tctgttttac tgaccgcgtt aatgttgttc
    43501 aggaagcgtc ccttatcggg tatatcagcg ccgttctggt ctttctgcag acgtttctct
    43561 gcattgtcat aggctgattt tactgccttt ggcgttgccg ccagcgtttc agacgtactg
    43621 ttggtcgcac tgctgagctg tactatcccc tttttcgtcg tacttgcatc ctcaagcgcc
    43681 acggcggatg caatatcctc tgcccgtttt gccgctgtct cggcgcgcgt tgccgcggat
    43741 tccgccgtac ttttgctctg tgctgccgcc gtcgcactgc cagctgcctc tgtcgccttc
    43801 gtggatgctg tcgtggcgct gcccttcgct gcggacgctt gtctggtcgc ctcatctttt
    43861 gaagcagacg ccgatgatgc cgatgacgcc gccgaactgg cggacgatgc ggcagccgtt
    43921 tttgaggatt ctgcgctggt ttccgacgct ttcgcgttcg tttcggatgt cttcgctgcg
    43981 gaagcagacc tcgctgctgc gctggcctgt tcagtggctt cgccagcctt cgttgtggct
    44041 gttgaagcag acgatgcggc gctttctgcc gattttccgg cagcggtggc actggctgag
    44101 gcctgtccgg cacttgttga cgcggcgctg gcagatgatg cagccgctgt ttttgagcct
    44161 gccgcagccg aggcgctctg tcccgctgcc gtttcagaag acctggcgtt cgtctcggac
    44221 gtttttgccg ccttcgcgga attgcctgcc gccgttgccg aggaagcggc actactggcg
    44281 cttgatgatg cgttcgtttc tgatgatttc gctgcctctt ttgaggccgc cgcatcccgg
    44341 gctgaggtgg ctgcttctga cgccttcgtg gtcgcggcgg atgcagaagt ggctgctgat
    44401 tgttgtgacg ctgccgcatt cgtttctgac gttttcgccg cagcggcact ggtagctgcc
    44461 gcgctttttg aggactctgc agcagcagca cttttcgatg cttcactggc ctttgttgat
    44521 gccgttcctg cgctggaaga tgctgactga gccgacgacg cggcctgtcc ggctgacgtg
    44581 ctggctgcgc gtgctgagcc tgcagcatca gtcgcatggg ttgccgcctc acgggctgat
    44641 gtgctggcat cgctggctga cttcttcgcg gctgccgtgt tctgtgccac cgcggacgcg
    44701 ttacgcgcca cctcttccac catcagttca aaacggcgca gtgcctcagg acgggcatca
    44761 tcctccgt
//
LOCUS       DAJTVJ010000017        44658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000017 DAJTVJ010000000
VERSION     DAJTVJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.017"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2161
                     /gene="torZ"
                     /locus_tag="OO448_000917"
     CDS             <1..2161
                     /gene="torZ"
                     /locus_tag="OO448_000917"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6336204.1"
                     /translation="RKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANG
                     PSAIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVE
                     VYEQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAID
                     PIRSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEE
                     YLRGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHW
                     MLVTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGM
                     RAIPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKP
                     EMIVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARN
                     DFDVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQN
                     KLIEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWL
                     APDEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSI
                     ANGDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIP
                     SSQLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(2326..3297)
                     /gene="cmoB"
                     /locus_tag="OO448_000918"
     CDS             complement(2326..3297)
                     /gene="cmoB"
                     /locus_tag="OO448_000918"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6336205.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3294..4037)
                     /gene="cmoA"
                     /locus_tag="OO448_000919"
     CDS             complement(3294..4037)
                     /gene="cmoA"
                     /locus_tag="OO448_000919"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6336206.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(4078..4473)
                     /locus_tag="OO448_000920"
     CDS             complement(4078..4473)
                     /locus_tag="OO448_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6336207.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(4526..4765)
                     /locus_tag="OO448_000921"
                     /pseudo
     CDS             complement(4526..4765)
                     /locus_tag="OO448_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5015..5545)
                     /locus_tag="OO448_000922"
     CDS             complement(5015..5545)
                     /locus_tag="OO448_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336208.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGELPNDIGIYGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLNKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            5736..5984
                     /locus_tag="OO448_000923"
     CDS             5736..5984
                     /locus_tag="OO448_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6336209.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            5986..8076
                     /locus_tag="OO448_000924"
     CDS             5986..8076
                     /locus_tag="OO448_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6336210.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRKVAEARIAIVSEVLKIAQQPGFSCAKAIRFLVNSLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKESDPLA"
     gene            8159..9091
                     /locus_tag="OO448_000925"
     CDS             8159..9091
                     /locus_tag="OO448_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6336211.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIAMAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            9094..9315
                     /locus_tag="OO448_000926"
     CDS             9094..9315
                     /locus_tag="OO448_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336212.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            9328..9582
                     /locus_tag="OO448_000927"
     CDS             9328..9582
                     /locus_tag="OO448_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336213.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            9584..9865
                     /locus_tag="OO448_000928"
     CDS             9584..9865
                     /locus_tag="OO448_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HCR6336214.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            9862..10134
                     /locus_tag="OO448_000929"
     CDS             9862..10134
                     /locus_tag="OO448_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336215.1"
                     /translation="MSKVVRIIFEYKEDVICKNSDGSTHRGVSLDIRSTGIKKKDNGP
                     AMIFGVVMLAESRNFAEFVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            10139..10432
                     /locus_tag="OO448_000930"
     CDS             10139..10432
                     /locus_tag="OO448_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336216.1"
                     /translation="MSKVRVIFEFNHVSHDEKLAGNDCVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPAIIQFLSAEFQASAQSFGADAIIKRHRVHKASGTLQ"
     gene            10444..10974
                     /locus_tag="OO448_000931"
     CDS             10444..10974
                     /locus_tag="OO448_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HCR6336217.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKQIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            11120..11653
                     /locus_tag="OO448_000932"
     CDS             11120..11653
                     /locus_tag="OO448_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336218.1"
                     /translation="MIDAKVLEGVKNWLCIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCGFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            11653..12168
                     /locus_tag="OO448_000933"
     CDS             11653..12168
                     /locus_tag="OO448_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336219.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFYFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVKWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            12172..12723
                     /locus_tag="OO448_000934"
     CDS             12172..12723
                     /locus_tag="OO448_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb domain-containing protein"
                     /protein_id="HCR6336220.1"
                     /translation="MMKLQPMGLKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTHISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            12720..13049
                     /locus_tag="OO448_000935"
     CDS             12720..13049
                     /locus_tag="OO448_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336221.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAAVLIVQYGHRQELRIGHMGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELREVNA"
     gene            13046..13396
                     /locus_tag="OO448_000936"
     CDS             13046..13396
                     /locus_tag="OO448_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336222.1"
                     /translation="MRSYIDNEKLETISDCLSLLAKIKETIEEIKFQLEYAPCGDDAW
                     RNAARKALAVFQKQRRAVEYRLAVLRQEEKERNIRRHERVNDFLVRELKERVPESVFF
                     ECEAIARSKALETD"
     gene            13412..13720
                     /locus_tag="OO448_000937"
     CDS             13412..13720
                     /locus_tag="OO448_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336223.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKFVSFMDKECDKRDMPEI
                     IMDDMILDSAVALSPLWIVHTLAEIAKRSDKQAAVAALRLLAELSCSVNPNTFFDEPG
                     "
     gene            complement(13682..14092)
                     /locus_tag="OO448_000938"
     CDS             complement(13682..14092)
                     /locus_tag="OO448_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HCR6336224.1"
                     /translation="MKIDYDEVLSILTTFQDAETPFLTLQDLGMAEAEGEEKDKKVFH
                     LMLLADNGSIVNGDMQSETPKYIGFFFHSLGVGFRNTPIMLTQQGHDLANALRKKPIL
                     ERIKKEFTDAPFDLIKEVTKSMLTRFVKERIGID"
     gene            14157..14669
                     /locus_tag="OO448_000939"
     CDS             14157..14669
                     /locus_tag="OO448_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HCR6336225.1"
                     /translation="MNRASLITLIHVAKRDLQLDHETYTSVLLAATGKPSCRDMSPGE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGGETALNRWV
                     KSQTAAQNDGEGVANWQWLEQQPALASDVLERLKRWHRRKMLAALDMSERAVMSYERV
                     CRLYEKSFPR"
     gene            14740..15162
                     /locus_tag="OO448_000940"
     CDS             14740..15162
                     /locus_tag="OO448_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6336226.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYQMSETQIYSVIRNQRRLHQERTQPRLF"
     gene            15234..15734
                     /locus_tag="OO448_000941"
     CDS             15234..15734
                     /locus_tag="OO448_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6336227.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENTTYIDP
                     TGTPTICYGHTGPDVKPGMTKTDEECLELLEKDMKWAFAAIDRHVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            15769..16197
                     /locus_tag="OO448_000942"
     CDS             15769..16197
                     /locus_tag="OO448_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336228.1"
                     /translation="MCRCAAAGCAVSAVNNPLPALIAGGCMAALGVMSVLAAVVWGMH
                     QKTQRLEDNNHVLVRERDEARLVLANQQRTLQLISQISKAATNEKQQNMQHSEEQQSI
                     VRRSLATVPAAAVPVPDDVADRVRRAVCEIRAGEAGADPR"
     gene            16181..16399
                     /locus_tag="OO448_000943"
     CDS             16181..16399
                     /locus_tag="OO448_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336229.1"
                     /translation="MPIPAEWLADCLVPPAPQPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETQRQHEFTDMAGTTAVPAGKTK"
     gene            16410..16637
                     /locus_tag="OO448_000944"
     CDS             16410..16637
                     /locus_tag="OO448_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCR6336230.1"
                     /translation="MDDSDCAQAVMERATERALCDRLTRKRRVAETPGRRVCADCGGD
                     IPAARLVAVPDAIRCVNCQNIMEAGHVGQHR"
     gene            16618..16929
                     /locus_tag="OO448_000945"
     CDS             16618..16929
                     /locus_tag="OO448_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HCR6336231.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VERVESRISGLPTQQEVNRLNIEITTLRGELKATNATLKSVSHQNELLLEQAVRKKHN
                     E"
     gene            16922..17212
                     /locus_tag="OO448_000946"
     CDS             16922..17212
                     /locus_tag="OO448_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6336232.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            17215..17796
                     /locus_tag="OO448_000947"
     CDS             17215..17796
                     /locus_tag="OO448_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HCR6336233.1"
                     /translation="MDKPARGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQIEFRIRQMLLGKA"
     gene            17807..18055
                     /locus_tag="OO448_000948"
     CDS             17807..18055
                     /locus_tag="OO448_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336234.1"
                     /translation="MATDNRSLMSSAHQKAFSLATQLPPETIKVCRELTELSIAGEIS
                     LEQLHSLLAEAATDELSGVDVMTEELESRIKSILTGKA"
     gene            18055..19719
                     /locus_tag="OO448_000949"
     CDS             18055..19719
                     /locus_tag="OO448_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336235.1"
                     /translation="MNKARETSPSLMQMTPPRKIDLSGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFADESQVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTDQEWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDNFISRAAWLRESEVLTWCEEH
                     LKPLLEKLNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALQERSRAMEGGYRDDDEAYHEFDRGGW"
     gene            19719..21308
                     /locus_tag="OO448_000950"
     CDS             19719..21308
                     /locus_tag="OO448_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HCR6336236.1"
                     /translation="MGRIIDTDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHSGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTTHTIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDRETPPEKQEKTEQTALSARLPEAKS
                     TPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLADALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDDTGD"
     gene            21292..22623
                     /locus_tag="OO448_000951"
     CDS             21292..22623
                     /locus_tag="OO448_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCR6336237.1"
                     /translation="MTQGIDLGYAATLPSKEAVAYFRVKGAHISWNWFETAADVHARS
                     FTAAKAARLDVVTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSDGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPSHSAL
                     NGLVFRYDDPFWKTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGEDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMQTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALMPEDFGRLPAMLAKPKAVLWDKRHKNLMYIVENKDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGQID"
     gene            complement(22625..22704)
                     /locus_tag="OO448_000952"
     tRNA            complement(22625..22704)
                     /locus_tag="OO448_000952"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            22745..23218
                     /locus_tag="OO448_000953"
     CDS             22745..23218
                     /locus_tag="OO448_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR6336238.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYTPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            23396..24520
                     /locus_tag="OO448_000954"
     CDS             23396..24520
                     /locus_tag="OO448_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HCR6336239.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHPAPAAGFVMASPENFRFS
                     EERGFEVRPDWNPPAIEHLRNNEFPWFSPVIGYDEITGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDTLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTLQTD
                     TVTVPDKKTATAALSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            24520..25467
                     /locus_tag="OO448_000955"
     CDS             24520..25467
                     /locus_tag="OO448_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HCR6336240.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGLNAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMRDFKADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNTTVSNEMKGKLELVVADYL"
     gene            25511..25864
                     /locus_tag="OO448_000956"
     CDS             25511..25864
                     /locus_tag="OO448_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336241.1"
                     /translation="MNEHHTVAATDDSGLQVSGDSPVTVLAEVRCSRAQFRRAGFLFM
                     RGRQQVDVTPEQLARLEAEPCLTVRILQTPGDDAGAVAGVVHAETGADLAEAGQDAPR
                     KKTGNNARQAKKARA"
     gene            25861..26280
                     /locus_tag="OO448_000957"
     CDS             25861..26280
                     /locus_tag="OO448_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HCR6336242.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDARKLNRALTDASA
                     LIDSYLSARYTLPLAVVPAVLVQHCCTIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTDGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            26277..26840
                     /locus_tag="OO448_000958"
     CDS             26277..26840
                     /locus_tag="OO448_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HCR6336243.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLISAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREVSRPGLYQIVARLITVLDGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFTEP
                     EGTPEFKAHISLPGATAAGEKAGPSEE"
     gene            26844..27131
                     /locus_tag="OO448_000959"
     CDS             26844..27131
                     /locus_tag="OO448_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6336244.1"
                     /translation="MTTIFIKPAPGCLIRDPDTMKPLAQEGEEKPFTPFWCRRLNDGD
                     VIVVEKAAEAAPAAASATTDAAGKPAGAAAEKPATEKPATEKPAASDKGTS"
     gene            27131..28633
                     /locus_tag="OO448_000960"
     CDS             27131..28633
                     /locus_tag="OO448_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6336245.1"
                     /translation="MINFDYIGDNNRIPLVQIEINNSMAVTGTPPQRQAVLLFGQAAM
                     KDTTIQGRGQLDVPVRITRASQARELWGRGSMIALMVEEFIAINPDTELYAIAQGVGT
                     GQSRACVMNIIGTALADGVLSVYIGGRRYLLPVSKGKKGKELVEELAKVINADTDAPF
                     TAIAAEVSGDNAEGLKGSMGVNARFIGECSAHDLRVNYYDGETTPAGLTLELVAPTQK
                     ATNPDITRSVSGMGNRQYNYIVMPYKDAANLKILADELLKRWGPVKMSDGMVWMAHTG
                     TFGTATSFGESRNDFLFTCTSIPKAPEPDYVWAASICAVCAPSLSTDPARPLQTLALP
                     ARMAPEAPARLTREERNSLLYTGMSTVTVAAADVVQIERQVTMYRQNAYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADNGTPVRPGQNIVTPEIMTLQLIALGEEWV
                     ELGLVENLDTFKKNLLVERNTKDRNRLDVMCTPDLVNQFRFMAAQIRFIL"
     gene            28642..29007
                     /locus_tag="OO448_000961"
     CDS             28642..29007
                     /locus_tag="OO448_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6336246.1"
                     /translation="MSGKQHQGTATIRVNGQEYATLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            29022..29501
                     /locus_tag="OO448_000962"
     CDS             29022..29501
                     /locus_tag="OO448_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6336247.1"
                     /translation="MTTRKKKTAVSDEAVMEAIRDVLEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAETEAERLMETRNGGPVLIASPALRGIALLRR
                     QIAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            29629..31686
                     /locus_tag="OO448_000963"
     CDS             29629..31686
                     /locus_tag="OO448_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6336248.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDKAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSEAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFARDVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGTGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLTAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            31673..33031
                     /locus_tag="OO448_000964"
     CDS             31673..33031
                     /locus_tag="OO448_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCR6336249.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPAVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINAPRRMAE
                     SLAGMFAVLTGLPGDPSLSLTGKAGTPSGSLTTNRDSMAEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTLGKLLDQVIRQNTTHVQDTDR
                     SGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGYTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSSRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            33015..34139
                     /locus_tag="OO448_000965"
     CDS             33015..34139
                     /locus_tag="OO448_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6336250.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRSKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEHNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            34129..34743
                     /locus_tag="OO448_000966"
     CDS             34129..34743
                     /locus_tag="OO448_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR6336251.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     VLDGTGKSDGLFTMSDAVIAGITYSGHVHQDNGKGSKTGAPENG"
     gene            34736..35173
                     /locus_tag="OO448_000967"
     CDS             34736..35173
                     /locus_tag="OO448_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6336252.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLSREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGE"
     gene            35173..36255
                     /locus_tag="OO448_000968"
     CDS             35173..36255
                     /locus_tag="OO448_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6336253.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIK
                     EGVLLQRSDGVVYRITTSLTGKAGTLNVSVEAESAGRAGNAPAGTKLTFITPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGDGDVQRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
     gene            36246..36806
                     /locus_tag="OO448_000969"
     CDS             36246..36806
                     /locus_tag="OO448_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6336254.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLASLCLGLSHSTARV
                     SWTGKQLLAERFPDTSRLLLEDWERYLGLPECDMAGATLTERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYQIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            36806..37687
                     /locus_tag="OO448_000970"
     CDS             36806..37687
                     /locus_tag="OO448_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6336255.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNRAANALPSDGTAVAANRLANAHTINGVPFDGTQD
                     ITISSGTVTAIRLGSVTAHMPGTWESWDLNLGGGNVLTGIKVQDVGKNTADNVGGVYY
                     RPLQYLLNGAWVTAASI"
     gene            complement(37719..38240)
                     /locus_tag="OO448_000971"
     CDS             complement(37719..38240)
                     /locus_tag="OO448_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6336256.1"
                     /translation="MELKNITRYYPENMPYGNNVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGVICSISQDASALYPAGFSVVEVDELPEGTDISGNWKFDNGIISRIPVNYAR
                     KLEAMRQSYLNQAYEKINDWRTELQLGTISDEDRAALTQWMAYISQVKKMELPAIKTE
                     AEFNAIKWPEQPQ"
     gene            complement(38292..38615)
                     /locus_tag="OO448_000972"
     CDS             complement(38292..38615)
                     /locus_tag="OO448_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6336257.1"
                     /translation="MPSDGTAVAANRLATPRNINGVPFDGTQDINITSGITQQDADSR
                     YIQDIRLGAENRQVMYGGAYYSAYGNVMAGLQIDSKVDASNDFIAFRPLQKLVGGTWI
                     TVSQL"
     gene            38731..39303
                     /locus_tag="OO448_000973"
     CDS             38731..39303
                     /locus_tag="OO448_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6336258.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALECAGCELFFEDRISGKTRERPG
                     LKRALKRLRPGDTLLVWKLDRLGRSMQHLVLLTEELRQRGVNFRSITDNIDTSTPMGR
                     FFFHIMGALAEMERELIIERTRAGLAAARAQGCHGGRPQKLTEKEREQIARLLANGES
                     RKQIALIFDIGLSTLYRYFPASPAPSPKVP"
     gene            39389..41443
                     /locus_tag="OO448_000974"
     CDS             39389..41443
                     /locus_tag="OO448_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6336259.1"
                     /translation="MSDSKWKMLSAMPGEFSVKVAGGTVAFIESPFRPSGNKGGITFA
                     DCVIRFSTKEPLWVMPVSGNPSAEITSSGVTGIIPITSDVAGTLTPSDWNAPDNAGPS
                     GSTNGSSSEPEYYFVVILAGQSNGMAYGEGLPLPDSFDRPDPRIKQLARRSTVTPNGT
                     SCTYNDIIPADHCLHDVQDMSGINHPKADLAKGQYGTVGQGLHIAKKLLPYIPQNAGI
                     LLVPCCRGGSAFTTGADGSFSEASGASADSSRWGAGKPLYQDLVSRTKVALAKNPKNK
                     LLAVVWMQGEADLASGSQQHNSLFTAMVQQFRTDLAPLAAQCVSGNATTVPWICGDTT
                     YYWKNAGTDKYEAVYGGYKGKEAQKIFFVPFLTDENGQSTPTNAPAEDPDIVAVGYYG
                     AASRTQGSFVSTQRDSHFSSWARRGVISDRLASAIMLHAGRTAELMRGQTVTPPDEKP
                     SPDTPSKPSTPPADTTTMSTLFAYRASESEGRLTPQGWAAGGGKAQIVDDAGASGGKA
                     MKLTKETGRAPWYLEHDAGNGADLLGKGGLVSCRFKLDGALTANQYALALYWPVSALP
                     QGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNTAQNTKLGSFGAFNNEWHTLGFRF
                     AGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITKNATYPVLIDSIVVEVN
                     NA"
     gene            41588..41770
                     /locus_tag="OO448_000975"
     CDS             41588..41770
                     /locus_tag="OO448_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR6336260.1"
                     /translation="MTFLQLILLYFCTAVCALYLVSGGYKAIRNYVRRKIEDAAAEKL
                     SKTAQVTPLPKDPTQP"
     gene            41806..42060
                     /locus_tag="OO448_000976"
     CDS             41806..42060
                     /locus_tag="OO448_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR6336261.1"
                     /translation="MPEIKGTVTEDLVKQALYSGEVNKLLKAQVRKDFEAQIDTYVDE
                     VLAKLIGPAVAASDADNEPKTDAQPEQPDPAQPGTDSTMM"
     gene            complement(42228..42488)
                     /locus_tag="OO448_000977"
     CDS             complement(42228..42488)
                     /locus_tag="OO448_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336262.1"
                     /translation="MIENAEIYNDYFSDYSDACKYSSSAGEFICFNFFIKSYVFPPQQ
                     EDGGVPPPEITLHRIASVSMMEQQANELYSILGTILQKRESD"
     gene            42778..42978
                     /locus_tag="OO448_000978"
     CDS             42778..42978
                     /locus_tag="OO448_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6336263.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQKASYIPYHQHA"
     gene            42932..43669
                     /locus_tag="OO448_000979"
     CDS             42932..43669
                     /locus_tag="OO448_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6336264.1"
                     /translation="MGNRKQVTSRIISTPELIHYNDNIVGYGSRELRVETISCWLARL
                     VVVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNNGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHETTFYELDGEWYHEICKNAIKRGGQRGEHLRTNIDRASVHKFRQFRYIRFLDKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            44498..>44658
                     /locus_tag="OO448_000980"
     CDS             44498..>44658
                     /locus_tag="OO448_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6336265.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSS"
BASE COUNT        10972 a        11142 c        12551 g         9993 t
ORIGIN      
        1 gagaaaaagc tatctcgata atccactgca accgacgaaa ggtcgtggcg aagataccta
       61 tgtacaggtg agctgggaac aagcattaaa actgattcat gagcaacacg atcgcattcg
      121 caaagctaac ggaccatcgg ctatttttgc cggatcatac ggctggcgct cttctggcgt
      181 gttgcataaa gcgcaaacct tactgcaacg ttatatgaac ctggcgggcg gttattccgg
      241 gcatagcggc gattattcaa ccggggcggc acaggtgatc atgccgcatg tggtcggttc
      301 tgtagaggtg tatgaacagc agacctcctg gccgctgatt ctggaaaaca gccaggttgt
      361 ggtgctgtgg gggatgaacc cgctgaatac cttaaaaatt gcctggagca gtaccgatga
      421 gcaagggctg gaatacttcc atcagctgaa aaaatctggc aaaccagtga ttgccattga
      481 tcctatccgc tccgaaacca tcgacttctt tggcgataat gccacctgga tcgcgccgaa
      541 tatgggcacc gacgtggcac tgatgttagg gattgcgcat accctgatga cacaaggcaa
      601 acacgataaa gtgttccttg aaaaatacac taccgggtat ccgcagtttg aagagtatct
      661 gagaggtaag agcgataata cgccgaagag cgcggcctgg gcggcggaaa ttactggcgt
      721 accggaagcg cagatagtca aactcgctga actgatggcg gcgaatcgca caatgctgat
      781 ggcaggctgg ggaattcagc gccagcaata tggtgaacag aaacactgga tgctggtgac
      841 gctggcggcg atgttagggc agattggtac accgggcggc ggttttggtt tctcttatca
      901 ttactccaat ggtggcaacc cgacacgtgt tggcggcgtg ctgccggaaa tgtctgcggc
      961 gattgccgga caggccagcg aggccgctga tgatggcgga atgagggcga ttcctgttgc
     1021 ccgtatcgtc gatgcgctgg aaaatcctgg cggcaaatat cagcataacg gtaaagagca
     1081 aacgtatccg aatatcaaaa tgatctggtg ggcgggtggc ggtaacttta cccatcacca
     1141 ggatactaat cgtctgatta aagcatggca gaaaccggag atgatcgtcg tttctgaatg
     1201 ctactggacc gcagctgcta aacacgcaga tatcgtatta ccgatcacca catcgtttga
     1261 gcgcaatgac ttgacgatga ccggtgatta cagcaaccag catattgtgc cgatgaagca
     1321 ggctgttgct ccgcaatttg aagcgcgaaa cgattttgat gtgtttgccg atcttgcgga
     1381 attactcaaa cctggcggaa aagagatcta taccgaaggt aaagatgaaa tggcgtggct
     1441 gaaatttttc tatgatgccg ctcagaaagg ggcccgtgcg caacgcgtca ctatgccaat
     1501 gtttaatgcc ttctggcagc aaaataaact gatcgaaatg cgccgcagcg agaagaacga
     1561 acagtacgtt cgttatggtg atttccgcgc cgatccggtg aaaaatgcgc tgggtacgcc
     1621 aagtggcaaa attgagattt actccaaaac gctggaaaaa tttggctata aggattgccc
     1681 ggcacaccca acctggcttg cgcctgatga gtggaagggt accgccgacg agaagcagtt
     1741 gcagcttctg accgcacatc cggcacaccg tttacacagt cagcttaact atgcggaact
     1801 gcgtaaaaaa tatgcggttg ccgatcgtga accgatcact attcacaccg aagatgctgc
     1861 tcgatttagt attgcgaatg gcgatctggt gcgtgtctgg aacaaacgtg gtcagatttt
     1921 gacaggcgcg gtggtgactg acgggatcaa aaaaggcgtg gtatgcgtgc atgaaggtgc
     1981 atggccagat ctggaaaacg gcttgtgtaa aaacggcagt gcgaacgtgt taacggcgga
     2041 tatccccagc tcgcagctgg caaatgcctg tgccggtaac tctgcgctgg tgtatatcga
     2101 aaaatatacg ggtaacgcac cgaagttaac ggcatttgat aagcctgctg ttcaggcgta
     2161 agaagtgaat agcgcccgga aacgggcgct gaggttatgc aaacgtaaaa ctgcctgatg
     2221 cgctatgctt atcaggccta catagtccct gcaatagatt gaattagcaa gggtttgtag
     2281 gccggataag gcattcacgc cgcatccggc agtaatacca gacctttacg gcttgcgcgc
     2341 aatcagcacc gcgcgtttag gcgcaggata accttcaact gtcttactcg gatcatgtgg
     2401 gtcgaggaaa tcggacagag attcggtaac catccattca gtgcgtcgct gctcttcagt
     2461 agtggtaacg cacacatcca caatgcggat atctacaaaa ccacacttct tcagccagtt
     2521 tttcagcgcc agcgcggaag ggatgaaata gacattacgc atttgcgcgt aacgatcgcc
     2581 tggtaccagc accgtgttct catcgccatc aataaccagc gtttccagca ccagttcacc
     2641 ctcattcacc agttgatctt tcaactgcca gagatgctcc agcggcgaac ggcgatggta
     2701 aagcacgccc atcgaaaaga cggtatcaaa ggctttcagt gccggaagct gttcaatacc
     2761 taacggtaac aaatgtgcgc gctgatcgtt acccagtagt ttacgcaccg cttcaaactg
     2821 gcacagaaac agttgcgtag ggtcgatccc caccgcgagg tgcgcgcctg cgccaatcat
     2881 gcgccacatg tgataaccgc tgccgcagcc gacatccaga atggtgcgtc cggttaaatc
     2941 agaaagatgg ggcagaacac gatcccattt ccagtcggaa cgccattcgg tatcgatgtt
     3001 gacgccatac agtgagaacg gccctttgcg ccatggcatc aggttccgca taagcgtttc
     3061 aatgcgttta atttgcccgg tgctcagtgg ctcttcgctt tcggcggtta cgctatgcaa
     3121 taaatccaga cgatacggtt taatttcagg cagaaattcc accgcgttgg accactgctt
     3181 aaacagcccg tgctgctgct cgcgctgcca gttagcaatc tgcgcgggca gcgtttcgag
     3241 ccagtgtgaa agatgatttt tggcaatcag agaataaaag ttaccaaagt cgatcatgca
     3301 gcgtcctctg cttttaatgc caccagtgaa ccaaagttaa agcactggaa ccacagctcg
     3361 ctatgctcaa aaccggcttt atgcaggcgt gctttatggg tttccacgga atcggtcagc
     3421 atcacgtttt ccagcatgct gcgcttctgg ctgatctcca gttcgctgta accgttggca
     3481 cgtttaaagt cgtggtgcat gttgaacaac agttcaccaa ctttggcatc ttcgaaactg
     3541 aatttttccg aaagcaccag cgcgccgccc gggttcagtc cttgataaat tttatccagt
     3601 aatgcctggc gctcggaagg ttccaggaat tgcagggtaa aattcagcac caccatcgat
     3661 gcgttttcaa tggcgatatc gcgaatatca ccttcaatga cgtctactgg cgtaggggct
     3721 ttataggcgt caatatgacg actgcagcgt tcaatcatcg ccggggagtt gtcgatggca
     3781 ataattttgc aattatcatg atgaatgttg cgacgcaccg agagcgtcgc cgcgcccaga
     3841 gaacaaccca gatcgtaaac ctgcgtacca ggttgaacga agcgctcggc taacatacca
     3901 atcatggaaa taatattgga atagccggga acggaacgct ggatcatatc cgggaagact
     3961 tcagctaccc gttcatcaaa ggtccagtcg cccagtctgg cgataggggc agaaaatagc
     4021 gtgtcgcggt gagacataac gtaaaaatcc gggaaaaaga aagtggcgta ttgtgcgcta
     4081 acgcagggag aaaaccaact cccagggcat ataccaaaga ttcgccagca ccatcagcaa
     4141 cagcgcacac caggtggcgc tcatgccgga acgtcgccag cgaaacagac ggtgatgaaa
     4201 accgtaataa tgcatcagac gaccagcaag caaaacgatg ccgcaaatat gcaccatcca
     4261 ggtttctgcg ccattcattt ccataaacag catcagcaca atcgcgatgg gaatatattc
     4321 caccgcgtta ccatgaatgc gaatagcgct ttgcagttcg ctaaaaccac cgtcaccata
     4381 ggcaacgcgg tactgcattc gcaggcgaac gacatcaaaa gagaacttca ttaataacaa
     4441 cgcacttaaa acggcatata gcgcgcttac catacaaact ccctttaaaa tggccgatgg
     4501 cacctgtcta tgataggtgg caaattcaga aaagagaaga ttgctgtgga atagccggaa
     4561 ctgctccgat ctctggaagc gttttacgta agtcttccca cagggtatgt accagttccg
     4621 gggcctgggc aatgtctggc gtatgtaaaa aaagataagg cgtagtggtc tgatgccact
     4681 gcgctaattt ctgtaaccag acctgaaata attcccggtt ttgcgtcata tcatcactac
     4741 cgataaaacg aatcagcgga tttgtgtaac caggtgaatc atttgatacc aacgagggtt
     4801 ttagtgaaag gaggtgaaat cacctgtaag atactgaaat aaaacaggtg atgtctggat
     4861 gagcgcttaa agtctatttg atatcgattg tgcaataatt aatcgtttat tctaaaatcg
     4921 gcaaaacttt tgatactgcg atctcaactt tttaaagcgt ttaaaacttc attaaatcta
     4981 tatggcaact aacatacggg ccagcctcgc caaactaacc cgctttagat tctaccggat
     5041 ttgattgcct acttgtctcg gttttttcca aaatctctct gacgcaatcg tctggcatat
     5101 ctacaatcat caacgcagcc aggcgcttcc tgccttctag ttgtaataaa tcctggtttt
     5161 ctttattcag cagtgtcaga accgtctcta cacctttgcg agcaaggaga ttggttaact
     5221 gttctcgttc gtctttcgta aggaatgaaa gcatctgtgt aagctgatct cccttatcac
     5281 cagaacaacc aaaattacca taaataccaa tatcgtttgg taattcaccc ttgccctctt
     5341 taagccactc aagactaacg ccttcacttt cagaaattcg tataaggata ctttctctgg
     5401 gggctggttg tgtttgtcct cccttgaaat agctgtttaa tgtgttgata ttgattcccc
     5461 acgctctggc agctgcactt cttgaagggt agcgctttgt aagcattcca atacgttcaa
     5521 taacaggttc ttttccctgc gacataaaga tacctcttgg tgcttttcta aggaaaggtt
     5581 tctttctatt tatccaattg aaaaataagg ataaaagcaa aaattagcca aaataccaaa
     5641 aataccttga tttggtattt ttggtgatgc attatttacc cgtacagata tccgcacgaa
     5701 tatccgcgcg ggtaactttt tagaatagag gaagtatgag tagaagagaa gtttcagatc
     5761 acgattggcc gcctgaaatc atcaaggcgc gcttacacat ggctggctta tcactgcgct
     5821 ctttatcttt aaaggctggt tacagcaggg attcgctgaa aagcgtatta cgtactcctt
     5881 gccgaccgta tcagcaaata attgctgatg ctcttggggt atcacctgaa gaaatctggc
     5941 ccagtcgata ccaggttaag agctatatga gaaaggcgtc atgatatgta tgtcattgca
     6001 aaagaactga ttggcgcgcc cggaatgcct gctacaacaa aaggtattcg ccaggcatta
     6061 caacgttacg tacaagggaa aagctgttgt tcccgtcgtc gctcaggctc taaagcaact
     6121 gaatacagca tcgactgttt acctgaagtg acgcagcagg cattacgtga acgctacgcc
     6181 ctgcaactga tgacgcaaaa agccgatgaa tcaccggctc cggtggtgac aaaggccaga
     6241 cgctcacctg ccgtggttga tgcggtggag gcatatcgcg gatcacccca actgatggtc
     6301 gaacgcctca atgccctgac tgaaaaccaa cgtaaagtgg cagaggcacg aatagcgatc
     6361 gtcagcgagg tgctgaaaat cgcgcaacaa cccggcttta gttgcgctaa ggctatccgg
     6421 ttcctcgtta acagcctggc acgatcacaa ctggacgagc gcattgtggc aatggttgag
     6481 acggcgaacg ccaaaaaggg aaacagccgc gcgttgagtg aaatcacgct gaaacgctgg
     6541 attgcggcct ttaacaaggc acagaacgcc gctgaacgcc tgcttttact ggcaccgggt
     6601 aaacgccagg aaataaaagc cgaagatatt aactggctgc ccgaatttct ggcgcagtat
     6661 cgccagtcaa acggccgacc aatgaccgag gcttacgagg attttgttgc tgaatggcag
     6721 caccggcacg ctgatgagcc ttatatgctc gatatcatgc cctcttatga caccattcgc
     6781 cgcgcaatga agaaactgcc ggaagtggtg aaacaaaaag gccgggtaac tggcagtgaa
     6841 taccgtcagc ttgagggatt cacgcgccgc gactggtcca gaatgccggt gaattatgtc
     6901 tggattggtg acggtcacgg catgaagctg aaatgcgcgc acccggttca cgggcggcca
     6961 ttcgcaccgg aagtgacctt tgttatcgac ggtggcacgc gctttgtggt gggctggagc
     7021 cttgacctgg ctgaaaatgt tttcgccgta gccggtgcca tacagcacgg tattcgccat
     7081 catggcaaac cgtttctgta ttactcggat aatggctccg gggaaaccgc cgacgtcctg
     7141 gataaggaga ttgtggggat actgccacga ctggggatta atcacccgac agggattgcc
     7201 ggtaatccgc agggacgggg cattatcgaa cggcttaacc gcacattacc gatgcgcata
     7261 gcccgtaaat accgcaccta tttcgggaaa ggagcagatc gcgagacgtt acgtaaaacc
     7321 aaccgcgatt tacgctcggc attcagtgcc ctgcaacagg gcaaacggct gaacgcccgg
     7381 cagcagtcag cgatgcgtga tttaccgtcc tggtctgaac tgattgatgc cattcgtgac
     7441 ggtgttgagt ggtacaacaa ccggccgcac gatgaattac ccatgaagcc gaacggtaaa
     7501 cattacagcc cggcggagtt cagaaaaaaa cgcctggcgg aagaggacac ggaaattgaa
     7561 tggctgtccg atgttgaact gcgggatatg ttccggccga tggtggagcg ccctgtaaga
     7621 cgctgtgaaa tacgctggct gaataatatt tactacgcgc ccgagctgcg tgacgagcat
     7681 ggccgtaagg tacttatcag ctatgacatt catgatgccg aacgaattac cgtgcgccgc
     7741 ccggatggca gcgtgatttg cgaggcggta tgggacggca ataaacgcga agccttccct
     7801 gtcagcgcgg aatactacaa acagcagcag cgccttaaag gcatgcgtaa acgcgcagag
     7861 gaaaaaatcc gtgatgccga ggatgaggtt gtcaacgtgc tggagcacaa gccgcaggag
     7921 ccctggctgg aaaacatata ccgccctgtg ggtaatacgg tgaccgttca gcaaccggtc
     7981 gctgacgatg aacctgatga agaatacgag cgtaatttcc agcggggatt gcaactgctc
     8041 gaagcgaaat taaaagaaag tgatccgctg gcctgaaata aaaaaataac ccgaggggtg
     8101 actcaggtta tttgaggttt cagtctcgga gaatgctgtc tcaagcgagg tgaataatat
     8161 gaccgatatt aacgatgtaa tcaagaccat tgatgaactt attgatggcg gcgtactgac
     8221 gcagtatgcc atcgccagag aggcgggaat ttccgacggc acattatcgg ctttccgtaa
     8281 gggaaaatat aaaggcgata acgctgctgt ggctgcttcc ctgcgctcct ggtatgagaa
     8341 ctggaataaa caaagcgcac tgccggaacc gccgcagttt gtggaaactc agacagtcca
     8401 ggagctgcgc gcactgtttc aggcggttcg cctgatgggc tgtattaacg ttattgtggg
     8461 cgtaccgggt gtgggtaaaa cggccactgc ccgtaattac tgccaggagc aaccaaacac
     8521 ctggatgatc accctgtcac ccgcgcactc cagcgtcacg gagtgtctgc tggagctggc
     8581 cgatgcgctg gggattgatt acacccgcgc gaacaaaggg gcattatccc gcgccatccg
     8641 ccgtcgcctg atgggaacgc gtggactggt gattgtggat gaggcggatc atcttggtat
     8701 tgacggtctg gagcaactcc gggcaattca ggacgccacg gggatcggga tggtgcttat
     8761 tggtaacccg cgcggattgt ttaaaggtgg acgccgcgcc tttgatgatt tatcgcgcct
     8821 gttcagccgt cttgcccgta caaaacaact tcgcaaggcc aaaaaggcgg atgtgctggc
     8881 cattgccatg gcgtggggga tcagtggtga ggccgagctg gccgtcatgc aggctatcgc
     8941 tgaaaagccg ggagcgttac gcgttctgac acatacgctt aaccaggcgt ggctcaccgc
     9001 cagcggtgaa ggcgcggcgc tgacagaaaa acatattaat gcggccttta aagaggttta
     9061 taccaaccct gaattactct cacaggtgtg attatggcgg tatttaatat tcctgatatt
     9121 tacggacgct tttacctggt taatttcgat aacgtgaagg tgatttcact ggccgaaaat
     9181 aaagaatgtg gcgatttact ttttgaattt aatgaccgca cacgaatggt gatatctgcc
     9241 ggacttgatc gcgaaggtgc gacagaagtt tacagcggaa tatgccgttc tgttggtgcg
     9301 aaacaagtca gctaaatgag gtgttatatg aatatgcaat cctgcggtaa caaaatgaat
     9361 ttattcgact ccctgaacag cgcgcgccgt ctgaccgaac ttgccggtgc ggtactggaa
     9421 cgcagtaagc gctatccgca acgttttgca ctgaaaacca cgccgccggt aggcaacgtg
     9481 cagggaaccg gtgaaattga aatcaccata cagaccaacg gcctgcgccg ccgtgtgaag
     9541 gccacccgca tcagcggctg cacggtttac tgggaggtgt gaggtgaaaa aaaatctcat
     9601 tgcatgggcg tggtcgagtg gtcttattga gtttggttac gtcctgccgg aaggtgcatt
     9661 gccgatagtt gccggaaagc ctgccacggt acggcatgtg attgaggtta tggcgcgtca
     9721 tggacgtgat gaacaggagc agttactggt tccggggata ccggaagcgg tgacggagga
     9781 agaagccttt aatgccatga ttcggttctg ccgtgaggtt agacgccggg tcagttatcc
     9841 aaacagaacg aggaccagag gatgagtaaa gtcgtacgca ttattttcga atacaaggaa
     9901 gatgttatct gcaaaaactc tgatggcagc acgcacagag gggtaagtct ggatatacgt
     9961 tcaaccggaa taaagaagaa agataatgga cccgccatga tttttggggt ggttatgctg
    10021 gcagaaagca gaaacttcgc agagtttgtg gcaatgaaag ccagtgcatt catgaaagac
    10081 agaggcatgg cttccggggt tattaacggt aatgaattta atcaacaggg gtaattccat
    10141 gagcaaagta cgcgttattt ttgaatttaa tcatgtttcg catgacgaaa agttggcagg
    10201 caatgactgt gttgaagtgc atgaaaagat tggagtggat gtgaaaacag aacgtgatac
    10261 ggataacagg ccgacgtcac tctgtgacgt ttatgcaagt attctccagt atcacagtcc
    10321 tgcaattatt cagtttctct cagcggaatt tcaggcatct gcacagtctt ttggagcgga
    10381 tgccatcatt aaacgccacc gcgtgcataa agcatcaggc acactgcaat aaggaaaaac
    10441 aaaatggcta aacgcgttac aaaattaaag gccgcagccg aggcggcacc gcagacccgg
    10501 gaagaagtca gccgtgatat ccgcaccctg ggagatattc agcgagaggc gctgcgcctg
    10561 gaaacggcga tgaatgacga agtggcagaa atcaccgccc gttatacgcc gcagattgaa
    10621 aatcttaaaa agcaaatcaa agtgcttttt aaagggattc aggactggtg caaaaccaac
    10681 cgtgatgagc tgacgaacgg cggcaaaacc aaaacagcca atctgaccac cggaacggtg
    10741 tcatggcggc tgggaacgcc atcatgcagc gtcagccgtg atgtggaagg tgtgattgaa
    10801 atgctgcgcc gtatggggct tgagcgtttc atccgcacga aagaggaagt gaacaaggaa
    10861 gccgtcctgg cggagccgga tgctgtgaaa ggcattgccg gtatcaaggt gaataaaggc
    10921 gctgaaagtt tttatgtcga gccttttgaa caggacgccg gactgaataa ataacaccgc
    10981 attaatcaat taaatatcac ttcattttaa ttatggcgct cgcgtcaggg gactgcttgc
    11041 gcctgaaaac agaaaataag ggttaagaaa tatgaaatac gtttatatcg tcattaataa
    11101 tgtggtgggg aaataaatca tgattgatgc aaaagtgctt gaaggggtta aaaactggct
    11161 gtgtatttat ggccgcctga cctgcggtgt actggctgaa aaaatgaata tgccgccatc
    11221 ctccatggtt tattttttgc gtgatgcggt tgatgcaggc gtgctgacgg aatgtaacgg
    11281 tttttatgat attccgcgtc cccgcccggt gcagccggtt cgtcgcaaat gcagccagga
    11341 atctgcggct gatgatgttc agtggtgcgg cttcagaaaa tccctgccat ggattgaggg
    11401 gcatgatatt ccgtcgatgg cgtgggaatt tgctcagggc gttctgacct gtgaaaccgt
    11461 ttatgtggtg gctgaagttg atgagcaggc catgaaagaa ggcgtgcccc agtttgtgat
    11521 ggcgtatatc gacatacgcc tgggcgtcat tatctgcggt ttaagcggct ggaatatcac
    11581 tgaacatgtt ctgcgctacc tgattgttga ccggacagca gcgcctgccg ggatatctgc
    11641 ggaggtggcg taatgttctt taaaacatca aacccttccg cgctggctgc gtggcaaaaa
    11701 taccagcagg actgccagaa agttaaggat gaggcaaaac gccttgaggc cgtgctgaat
    11761 gttgcgtgcc ggtcggtatt tgtatccggt atcagtggat tttactttaa gggactgcgt
    11821 tttacggagg acaaatatcc ttttcatcgc gacttatggc gaaaaccgac tgcgtcgaat
    11881 ggctggagct gcacaccgcg cacatcacgt atccctaaaa ccctgcgtgt tgcctctgat
    11941 gaactgaaca gtctgtggcg tgaatattcg cccgtcacgt atgccagaac cgatgcgctg
    12001 ttgttctggt tgggtattga tttctcggca atcctgtttg gccctgtgaa gtggttctgc
    12061 gttgacgatg tgatttacct tcagtgcggc gtaaaacccg caaaacagaa aatgaccgaa
    12121 atactgtctg atgagtttta tgctgctgaa aagcgagtca gggggtgatg catgatgaaa
    12181 ttacaaccca tgggactaaa aggtcgcgcc cccgctcatg tccgtccgtg gacacctgaa
    12241 gaagatgcgc tgctgattgc gctttattca tccaccccgg ttaaggatat tgctgccaga
    12301 ataaaaagaa ctgtctgggc tgtatataac cggactggtg tattgcgcag ttcatacccg
    12361 gagttactga aatataaaca cccaagattt acacctgatg aagataagtt tattcgaaaa
    12421 aatgccagaa caatgacctg ccagcaaatg ggagaatatc tcggacgtaa taaagattct
    12481 gtcagatgtc gggcaggaat gattggtgct ggattaacaa agtgcggaga gttacgcccc
    12541 ggaacgcaca tatctgatga tgatgtacgt cttatacgtg cgctgcgtga ttccgattac
    12601 ccacgccgtc tgtcattccg ggaaattggc gaaaagtttg gaatatctga acattctgct
    12661 cacgcagttt attaccgtcg ccggactgcc gaggacgctg tattacggga gttaacgcca
    12721 tgataacgac cttatttgtt gaatcagatg aacctcttgt gtgtgccgcc ggaatgccgc
    12781 tctgtggcgg aacgctgacc ggtgtttatt tcggggattt acgcggttat ccctggcatt
    12841 cactgaatga tgctttccca cctgatatgg aggctgccgt gctgattgtt cagtatggtc
    12901 accgtcagga gctgcgcatc ggccatatgg ggtatgaagg cttttttgtt gatgaagaaa
    12961 ccggagcctg ccttgaagat gaagacggac aggtgacgca ctggtgtcat atttcggctt
    13021 taccggaatt acgggaggtg aatgcgtgag aagttatatt gataatgaaa agctggagac
    13081 aataagcgac tgtcttagct tgctggcaaa aatcaaagaa actattgaag agattaaatt
    13141 tcagcttgaa tacgcaccat gtggcgatga tgcatggcgt aatgctgcca gaaaagcact
    13201 ggctgtcttt cagaaacaac gccgggctgt tgaataccgc cttgctgtat tgcgccagga
    13261 ggaaaaggaa agaaatataa gacgtcatga acgcgttaat gatttccttg ttcgtgaatt
    13321 aaaagagcgc gtgccggagt cggtgttttt tgaatgtgag gctatagccc gtagcaaagc
    13381 actggaaaca gattaagcag gcaggtgaat aatgagtatt gaattaagat cgtcttatga
    13441 atatcgcaaa atccttatcg ccggaggcat gaaaccggaa gatgcagaga aattcgtttc
    13501 ttttatggat aaagaatgtg acaagcggga tatgccagaa attattatgg atgacatgat
    13561 tctggattca gccgtggcgt taagtccgtt atggattgtg catactcttg cggaaatagc
    13621 taaaagaagt gacaagcagg cggctgtcgc cgccctgcga ttactggctg aattaagttg
    13681 ttcagtcaat cccaatacgt tctttgacga acctggttaa cattgatttg gtaacttctt
    13741 taattagatc aaatggtgcg tctgtaaact cctttttgat tctttcaagg attggttttt
    13801 ttctcagggc gttagctaaa tcatgcccct gctgggtaag cataatgggc gtgtttctga
    13861 aaccaacgcc cagagaatgg aaaaagaaac caatatattt gggtgtttct gactgcatat
    13921 caccattaac aattgagcca ttatcagcga gaagcataag atgaaaaact tttttatcct
    13981 tttcctctcc ttctgcctca gccatgccca gatcctgtag tgtaaggaaa ggtgtttcag
    14041 catcctgaaa agttgtgaga atacttaata cttcatcgta atctattttc atatcgatac
    14101 ctttagtttt atccgggcgg cgttgccatg ccgcctgttg ttacggagac acgataatga
    14161 atcgcgcttc cctgattacc ttaatacatg tcgcaaaacg cgatctacag cttgaccatg
    14221 agacctacac atccgtacta ctggcagcca ccggcaaacc cagttgccgt gatatgtcac
    14281 ccggtgagtt atcccgcgtg ctggatgttt tcaaaaaacg cggttttaaa gtgcgtcaga
    14341 agccggttaa ccgggcctta aaaccgggta cggtgaccgc caaaattcgc gccatctgga
    14401 aagtgatgca ccggcaggga tttatctctg atggcgggga aacggccctt aaccgctggg
    14461 tgaaatcgca gacggccgcg cagaacgacg gcgaaggtgt ggctaactgg cagtggctgg
    14521 agcaacaacc cgcgctggcc tctgatgtgc tggagcgtct caaacgctgg caccgccgca
    14581 aaatgctggc agccctggat atgtcggaac gtgcagtgat gagttatgag cgtgtttgca
    14641 ggctgtatga aaaatcattc ccccgttaac cccaaatccc gccacagcgc gggattttta
    14701 ttttaaactt gccctgtgta atgatgaccg gaggcgggta tggctgaaac acagatgagc
    14761 atgtttggtg acagcgaaca gctgcacgcg ctgattgacc gtctggatga tattcctgat
    14821 gatgagctga aaaagaactg gcccggcacg ctgcgggacc tggttgatgt gatcagtgct
    14881 gaactgcacc gtcaggggat tgaacctgct caggcgggaa tgcttgcccg taaggttgca
    14941 gccgcacagg ccgggtacat gggcgggcgt ggttattatc tcccggtcgg tgaatccctt
    15001 ttcaccgaac tgcgcaataa tgaaattttt tcccgctgga gccagggcga aaaaattgaa
    15061 accctgcgcc gccactacca gatgtcagag acgcagattt actccgttat ccgcaaccag
    15121 cgtcgcctgc atcaggagcg cacgcagccc cggcttttct gatattccgc aaatcctgcc
    15181 cccttttcct cacggttacg ctgactcaga gaacaccatg agacagcgta acaatgccaa
    15241 aactccccgc acccctgcgt aaaaagctga ttgcccttgt tctggccgga gccgggacgt
    15301 tcaccattgc cacgcactac accggctact gggaagggaa agaaaacacc acgtacattg
    15361 atcccaccgg cacccccacc atctgttacg gccataccgg cccggatgtg aaaccgggga
    15421 tgacaaaaac tgatgaagag tgtctggagc tgctggaaaa ggacatgaaa tgggcctttg
    15481 cggctatcga tcgtcatgtt caggtgccgc ttacccgtgg tcagacggtg gcgctggcct
    15541 cctggatttt ctgggccggt gaaacgaact ttcgcaactc cacgctcctg cgccttatca
    15601 atgccgggca gatgcccgcg tcctgtaagc agtatatccg ctggatttat tcaaaggggg
    15661 taaaactccc cggtcttgag gcccgccgtt cggcggatga atggttatgt cgttacgatt
    15721 tgccgaaagt ctgaaccgct tctggcgacc gctcatgatt gcgctgctgt gtgccgttgt
    15781 gctgctgcgg ggtgtgctgt gtctgcggtg aacaatccct taccggccct gattgccgga
    15841 ggctgtatgg cggcgctggg ggtgatgagc gtgctggctg ccgtggtatg gggaatgcac
    15901 cagaagacgc agcgccttga ggacaataat catgtgcttg tgcgtgaacg ggacgaagcc
    15961 cgtctggtgc tggcaaacca gcaacgcacc ctgcaactca tttcacaaat cagcaaggcg
    16021 gccacgaatg aaaaacagca gaacatgcag cacagcgagg aacagcagag cattgtccgc
    16081 cggtcactgg caacggtgcc tgctgccgct gtgcctgtgc ctgatgatgt ggctgaccgg
    16141 gtgcggcggg ccgtctgtga aatacgtgcc ggtgaagccg gtgccgatcc ccgctgaatg
    16201 gctggctgac tgcctggtcc ctcccgcgcc gcagccgttc acgtttggtg catcggtcac
    16261 ttacaacctg caactgctgg cggtgattaa aaactgcaac gtggacaagg ccagcatccg
    16321 ccgtctggag acacagcgac agcatgaatt tactgatatg gccggaacga ctgctgttcc
    16381 ggcaggaaag acgaagtaag gaaaaaggta tggatgattc agattgtgct caggcagtca
    16441 tggagcgtgc aacagaacgc gccctttgtg accggctgac acgtaagcgc cgggtggcgg
    16501 agacaccggg caggcgtgta tgcgcggact gcgggggtga cattccggct gcccgtctgg
    16561 ttgccgtgcc ggatgccatc cgttgtgtga actgccagaa catcatggag gccggtcatg
    16621 tgggtcagca tcgttaaaga ttatgtcgtg ccgatcctgt cggcaacggc cacagccggg
    16681 gggatcttca tggcgctgat gcgcaaaacc tttgtccccc gcgaggcctt tgaaaaactc
    16741 tccgaccggg ttgagcgggt ggagagccgg atttccggcc tgcccacgca gcaggaggtg
    16801 aacagactca atatcgaaat caccaccctg cgcggtgagc ttaaggccac caatgccacc
    16861 ctgaaatccg tctcccatca gaacgaactg ctgctggagc aggctgtaag gaaaaaacac
    16921 aatgagtgat ttcattactg aagatcagcg tcttgtcatt ctgcggtcgc tggcggatta
    16981 caacggcgaa ctgggcgaat ccgtgcttca ggactgcctg gatgattacg gtcaccgcgt
    17041 gtcccgcgac accgttcata cccacattgc ctggcttgcc gagcagggac tggtgcgtaa
    17101 gcgcacgctt gttaacggtt atttcatcgc tgaactgacc ggccggggtc aggatgtggc
    17161 ggaaggccgc gccaccgttc cgggtgtgaa gaaaccacgc gcaagggggt aacgatggat
    17221 aagccagcgc gtggccgtgt acgcaaggtg gatttgctcc ccgacagcat ccgtaagccg
    17281 ctgctggaaa tgctgcgtga aaaacgtctg acgcaggtcc agatccgcga ggaaatcaac
    17341 cgtctgatcc gtgaagcagg tctgccggaa gagcagcagc tctcaccggc ggccatcagc
    17401 cgggaagcct cccgtaatga gctgattgcc cgtaatctgc gtgatttacg cgagcagaca
    17461 aaagccatga tggccgaact gggggataag ccgaccggtg aaaccacggc actgattctg
    17521 gagatgtccc gtgccctgat gtaccgccgt ctgcgtgccg ccaccgaatc cctgaacagc
    17581 gacagcgatg tggatatgcg ccttattaag gacatcctcc tgtcggcaca gcgtgcagaa
    17641 agtgccgccg agcgcagcat taagcgtgaa aaagaaatcc gggccgcatt tgctgaagag
    17701 atggcgaatg cggtcaccga tgagttgcgc ggcgtggatg gcatgagcga gcagatcgaa
    17761 ttccgtatca ggcagatgct gctgggtaag gcgtaggagg tgaatcatgg caacagataa
    17821 ccgctcactg atgagcagtg cccatcagaa agcattcagt cttgcaacac agttacctcc
    17881 tgaaaccata aaggtatgtc gtgagttgac tgaactcagc attgccgggg aaatatccct
    17941 ggaacaactg cattcgctgc tggcagaggc tgccactgat gagttgagcg gcgtggacgt
    18001 gatgactgaa gagcttgagt cccgaatcaa aagcattctg acaggtaaag catgatgaac
    18061 aaagccagag aaacgtcgcc ttcactgatg caaatgacgc ctccccgcaa aattgatctg
    18121 tcaggtgaaa aagccctgct gggcgtggat gtgccggaca gccttgattt gcccggtgat
    18181 atgccggtgt ttctggatta tcaggcccgc tggtttgcag atgaaagtca ggtctgcatc
    18241 gcggagaaat cccgccgcac cggtcttacc tgggcggaag ccgggcgtaa cgtcattact
    18301 gctgcaaaac cgaagcgtcg tggtggccgc aatgtgtttt atgtgggatc aaagcaggag
    18361 atggcgctgg aatatatttc tgcctgcgcg ctgttctccc gtgcctttaa ccagctggca
    18421 gatgctgatg tgtatgagca gacattctgg gatcgggata aaaaagaaga aattttgacc
    18481 tacatgatcc gctttccgaa cagtggattc aaaattcagg cattgtcttc ccgtccgtca
    18541 aacctgcgcg gccttcaggg ggatgtggtg attgatgaag ccgcgttcca cgagtcactg
    18601 gatgagcttc tcaaggcggc aatggcgctc actatgtggg gcgcgcgcgt gcgtattatc
    18661 tccacgcata acggcgtcga taatctgttt aatcagtaca ttcaggaagc ccgcgagggg
    18721 cgcaaggatt acagcgtcca ccgcataacc ctggatgatg ccattgcgga cgggctgtac
    18781 cgtcgtatct gttacgtcac cgatcaggaa tggtcaccgg aaagcgagca gaaatggcgt
    18841 gatgatctct acaaaaacgc cccgacccgt gaggatgccg acgaggaata cggctgtatc
    18901 ccgaaaaaat ccggcggtgc ctatatcccc cacgcgctta ttgaaatggc gatgatccgc
    18961 gacatcccga ttctgacgtt tgaagccccg gacaacttca tcagccgtgc cgcatggctg
    19021 cgtgaatcgg aagttttaac ctggtgtgaa gaacatttaa aaccgctttt agagaaatta
    19081 aatccccgtt cccgcttcag cttcggggaa gactttgccc gcaccggtga cctgtcctgc
    19141 tttgtgctgc tggaaatcac cgaatccctg gcaaagcgcg aggtgtttcg cgtggagctg
    19201 cgcaacctgc cgtatgccca gcaggaacag gtgatgatgt acatcctgac ccgcgttccg
    19261 gcgctggtcg gtgcggcgtt cgacgccacc ggtaacggcg gctatctggc agaggccgca
    19321 ctgctggcct ttggcccgga catcatcgac tgcgtgatgt tgtcgccgaa gtggtacggc
    19381 gagtggatgc caaaactgaa agccgagttt gaagatcaga atatcctcgt cgcccgccat
    19441 cagaccacgc ttgatgattt gcgccatgtg aaggtagtga atggtatccc gcaaatcgac
    19501 aaggggcgca cgaaggacca gaacgcaacg gctgcgaacg cccgccgcca tggtgatttt
    19561 gccgtggcgc tctgtatggc aaaccgggcg tcatacatgg agggcttcat cctggatgaa
    19621 tcagcctgtc aggcgctaca ggagcggtcg cgggcaatgg agggcggtta tcgtgatgac
    19681 gatgaggcat atcatgaatt tgatcggggg ggctggtagt gggacgcatc attgatactg
    19741 acggaaaacc tttctccttt gacccggaga tgcagagtgc cgcgctggat attccgcaga
    19801 ttgccagccg ttatattgaa catccggctt cgggtatcac cccgaaccgg gcggcgcagt
    19861 gccttcgcgg ggctgaacgt ggcgatctga ttgcccagtc cgatctggcc gctgatattg
    19921 aagaaaagga cacccacctt tttgcggagc tgggcaagcg acgtcttgcc attcaggggg
    19981 tgccatggag tattgagccg ccaccgaacg ccagcgcgaa tgagaaaaag gacgcggaaa
    20041 tgctcgacga atatctgcat tccgcagact ggtttgatgc catgctgttt gacgccacgg
    20101 atgccatcct gaagggctat tcctgcatgg aaattgagca cgggatgctc ggtaaaatgc
    20161 acatcatccg cgccatccgc tggcgtgaca gcgggcattt ctgccttaac ccggatgatt
    20221 tgagcgaact gcggctgcgt gacggcagcc attccggggt ggcgtttcag ccctttggct
    20281 ggatagtgca tcagtcgcgt tcacgcaccg gttacggtgg tgcaaccggg cttgtccgga
    20341 cgcttatctg gccgttcatt ttcaaaaact attccgtgcg cgatctggcg gaatttctgg
    20401 aggtgtacgg cctgccgatg aaggtcggga aatacccgtc cggggcaaca ccggagcaga
    20461 aaagtgccct gatgcgggcg gtgatggata tcgggcgacg tacaggcggg atcatcccgg
    20521 ccgggatgtc gctggagttt caggcggcag cgaacggtca ggccgatccg tttgaaacca
    20581 tgatttcgtg gggggagcgt tccatctcca aagcaatact cggcggcacg ctgaccacgg
    20641 aagccggaga caaaggcgcg cgctcactgg gtgaggtaca taacgaggtg cgccgggaaa
    20701 tcagggattc tgatttacga cagctggctg caaccctgaa ccgcgatctg gtgtatccgc
    20761 tgtacgccct gaacaccacg cacactattg atatccgccg tctgccacgt atctgcttcc
    20821 agacaaaaga accaggtgat atcaccaaaa tcaccagtgc ggtgatgcag ctcagtacgg
    20881 ggatggatat ttctgatccc tggatacgtg agcagaccgg tattccgcag cccacgcccg
    20941 gtgaagccat attccgtgtc cgtcagagtg gcaatgaacc ggctcagacc gacagggaaa
    21001 caccaccgga aaaacaggaa aaaacagagc agacggcgct gtcagccaga ctgccggaag
    21061 cgaaaagcac gccgcgtgat gaactggatg atatggggga cgcggtgcct gcccgccgct
    21121 tacaggaggc catcgacccg gtactggagc cggtcattga tgccatcaga acgcgggggc
    21181 tggcggatgc gctggcagac ctgcctgccc tttatcgtga aatggatgat tcccgcctga
    21241 tgacgctgct cagtgatgcc atgtttgctg cggaaatgaa ggggatgctg gatgacacag
    21301 gggattgatt taggttatgc cgccacgctt ccctcaaaag aggcggtagc atacttccgt
    21361 gtcaaagggg cgcatatcag ctggaactgg ttcgaaacag ccgcggatgt tcatgcccgc
    21421 tcattcacgg cagcaaaagc ggcacgcctg gatgtggtga ccacgttaca ggccgaagtg
    21481 cagcgggcca ttgatgaggg aatttcacag aaagcattta tccgcacgct gacgccacgc
    21541 ctgcaaaagc tgggatggtg gggaaagcag attgtggtgg acagcgacgg taacgcggaa
    21601 gaagtgcaac tgggcagtcc ccgtcgtctg gcgctgattt acaacgtgaa cacccgggtg
    21661 gcttacaatg ccgggcgtta cacgcagatg atgaacaaca cggacacgca tccgttctgg
    21721 cagtatgtgg cggtcatgga cagccgtacc cgcccgtcgc attccgccct taacggtctg
    21781 gtattccgct atgacgatcc gttctggaaa acacactacc cacctaatgg ctggaactgc
    21841 cgctgccgtg tgcggccatt gtctcaggct cgtctggatg caatggggtt atccgtttca
    21901 tccggtgagg atcatctctc cacccgcaat gttgaggctg gcgtggataa acagaccgga
    21961 gaagtcagag aaatgccggt gaccacatat tcagatggca ccagaaccat gacaccggat
    22021 gtgggctggt catataaccc cggctcggca gcgttcggca cagaccaggc gctgatccgc
    22081 aaactgatcg aggtgaaaag cccggcgtta cgggaaatgg tggttcagga gatgaacaac
    22141 agcccggagc ggcagctggc gttccgcatc tgggcaaaaa acatcatgca gacccggcga
    22201 ggtggtaacg atatccgcac gctgggcttt atgactgaaa gcattgcgca ggcagtggaa
    22261 agccggacgg gaacgccacc ggcccgcctg ctggcgatga gcggtaaaaa tgtgctccat
    22321 gcagacagtg tgaaacatca gaatgacggc atcgccctga tgccagagga cttcgggcgc
    22381 ttaccggcaa tgctggcaaa gccaaaagcg gtgctgtggg acaaacggca caaaaatctg
    22441 atgtatatcg tggaaaataa ggatggcagt gtgcagattg ccgttaatgc accgtacagt
    22501 ctgaaacgtc agcccgacaa actggatgtg attgtgaatg cttaccgggt gatcaatatg
    22561 gataagctga aatcggatat ccggggcgga atgcttgagg tgctggaagg acaaattgat
    22621 taacggtcag tggcgggggt cgaacccgca tacatgatcc acccggaggc aggcaccgac
    22681 tttaccagtt aagcgtacac tgaccgcctg tttattttat attcatcctt tatgcggagg
    22741 caatatgtca tccattgatg ccgctgtcgt tgttgacgtt acgcgtctcc agcgggtgtt
    22801 tgcccggctt cagtttgtgg gcggcggaaa agacctggcc cgcagtgtgg cggcgagtct
    22861 tctgtcatcg tcagaaatgg cgtttgaaca ggaaaaagag ccggacggcg aacgctggca
    22921 tgactggtca gatccttacc gcaagtggcg tacccgtaag ggatacacgc ccggcaaaat
    22981 cctgacactg aacggcgatc tggcccgccg cctgaccacg gattatggcg atacctgggc
    23041 gctgattgga tcaaatgaac cttatgcggc cattcatcag tggggtggcc tgcctggtat
    23101 gccaccggga ccggcggcca ttggcgcacg tccgtatatg ggctttgatc aggtggcaga
    23161 acaggagatc atggacgaaa tcagaaaacg ctttaaaaag gccacagaaa cgccgtaatg
    23221 gtttaaggta tacaaacgca ttaccttacc ctcttcaggc gcgtgtcgtg attttctaac
    23281 ctgtatttaa cgggctttaa aatctgcgtg aagcgccttt ctcatttttc ccctgtcagc
    23341 actttctgaa gtcccgcaaa accggccccg aaaaacaccg gcttatgctg ccggaatgaa
    23401 gacgaaaaac acgcccaaac ttgcttatgc cattctgaat gccatcagcc tgtccgcaga
    23461 cggggacggt gactggtgtc agatcatgcc agccggtcgg gtaaaggccc gtgacggtcg
    23521 cccggaaaaa ccggcggaag gctggctgat taaccatgcg gccgttgagc gcatggtatc
    23581 ccgggttgtg gcgctcaatc agccggtaaa aattgactac aaccaccaga ccctgattaa
    23641 ggggcatccg gcaccggctg ccggttttgt catggcctca ccggaaaact tccgcttcag
    23701 tgaagagcgg ggttttgaag tgcgtccgga ctggaaccct ccggccattg aacacctgcg
    23761 caataacgaa tttccctggt tttccccggt gattggttat gacgaaatca ccggtgaacc
    23821 tgttgagctt cggatgctgg ctattaccgg tgaccccggt ctgactggca tgaatcctgt
    23881 cgccgcactg tcggcggatg acctttataa cgccttaaac cctcctttaa aggacacctc
    23941 catgaatgag caattacgcc agttgctgac ggcgctcggt ctgaccgtgg ccgacggtga
    24001 cgaatttaca ccggaactgg gcacggcggc gctgtctgcc cttaccggga tcaaaacccg
    24061 tgcggatgcg cacgacaccc tgaaaacgca ggttgccagc ctgtcggcag agctggaaac
    24121 cgcaaaaggc acaccaactg gcggcactat tgatctgacg aaatacgtgc ccgttgagac
    24181 gtataacgcc ctgcgtaccg aatatgtcgc gttgtcggca caacacggca gcaccacgct
    24241 ggagcagttg ctggataagg ccgagtccga aggacgcatt ttcaaaagcg aacgcggata
    24301 catggaagga ctcggacaac agattggtgt tgcggcactt tctgcacagc ttgacgcccg
    24361 ccagccggtt gcggctctga ccactcttca gaccgacacc gtaaccgtgc cggataaaaa
    24421 gaccgccacc gctgcgctgt cggctgaaga tatcgccgcc gcccatctgc tgggtaaaac
    24481 cgaagccgag ttcctgaaaa tgaaagagga aatgcaataa tgcctacccc gatcacaccg
    24541 gcgatgatca ccgccctgat gacgggctac cgttctgatt ttcaggccgg gatgtccatg
    24601 gctccgtctc agtaccagaa aattgcgatg accgtaccgt ccacgtcgaa atccaacacc
    24661 tacggctggc tggggcagtt cccgcagttc cgtgagtgga tcggctcccg cgtcatcgag
    24721 aagatgaaag cctatggcta cgccatcgtg aacaaaacct ttgaaggcac cgttgccatt
    24781 agccgcgatg actttgaaga cgacaatctt ggcatctact ccccgttgtt ccaggagatg
    24841 gggcgcgctg cggcggcaca accggatgaa ctggtctttg ctgcactgcg tgatggtctg
    24901 aatgcggcct gctatgacgg ccagaacttc tttgataccg aacaccctgt ttaccccaaa
    24961 gtggatggct ccggtgatgc acagatggtc agcaatatgt ttgtggcaaa aaccggctct
    25021 gtcggtgcac aggcggatta cagcggcccg gcctggtatc tgcttgactg ctcccgcgcg
    25081 gtaaaaccgc tgatttatca ggatcgccgc aaggccgaac tggttgccca gacaaaagtc
    25141 gatgaaggcc gtgcgtttac tgataacgag tttgtgttcg gtgcttccgc ccgtcgcaat
    25201 gtgggctacg gcttctggca gatggcctac atgatgcagt caccgctgac gctggatgcg
    25261 ctgtggcacg gctggtcagc catgcgtgac tttaaggctg acggtggccg caaactcggc
    25321 atcaaaccca cccatattgt tgtccccacc tcgctggaaa aacaggcggt gcagctgctg
    25381 gagcgcgagc tgttcgcgga cggtaacacc accgtctcta acgagatgaa gggcaagctg
    25441 gagctggttg tcgcggatta tctgtaaacg gtgagccggg cttacggccc ggcccctgcg
    25501 gagggcaaat atgaatgaac atcacactgt ggcagcaaca gatgattcag gccttcaggt
    25561 cagtggtgac agtcctgtca cggtactggc agaagtgcgt tgcagtcgtg cgcagtttcg
    25621 tcgtgcgggg ttcctgttca tgcggggacg ccagcaggtt gacgtcaccc cggagcagct
    25681 tgcccgactg gaagcggagc catgcctcac tgtacgaata ctacagacgc ctggtgatga
    25741 tgcgggagcc gtggcgggtg tggttcatgc agagaccggt gcagatttag ccgaagccgg
    25801 acaggatgct ccccggaaaa agaccggcaa taacgccaga caggcgaaaa aggctcgcgc
    25861 atgaattacg ccaccgaaac cgatatgcgg gcacgttacc gcgaggattt gctcaggcca
    25921 ttactggccg tgccccgttc ggatgagccg gacgcgcgca agctgaaccg ggcactgacg
    25981 gacgcgtctg cccttatcga cagttacctg tctgcccgtt acacgctgcc gctggcggtg
    26041 gttccggcgg tccttgttca gcattgctgc acgattgcct tttattacct gtgcgatcag
    26101 cgggcatccg atcaggcgcg tgaccgttac cgtgaggcgc tggcctggct gaaagatgtc
    26161 atgaacggta acgtgccggt cggcgtggat acagacggtg cggcccctga atccggggat
    26221 ttaccgcagg tgcagtctga tgcggcggtg ttcgggcgca accagaaggg ctttatatga
    26281 ttacggaaac cgagcaagcg tacatcgccc gtatccgtga gtatttcggg aatgaactgg
    26341 tgtcagttga cacgcatccc ggcgactgga gcgacagtgt gctgcgcacc atgctgatta
    26401 gcgccccggc gatctacgtt gcctggctgg gtgccggtga aggccgtacc cgtgggcgtc
    26461 tggtcagcca ctgggtgttc tacgtcatcg gcgacatgct caatggccgt gaggtcagcc
    26521 gtcccgggct ttatcagatt gtggcccgcc tgattaccgt gctggacggc ttcagaaccg
    26581 aaaaaacctc accgctttac tttgaaaagg cggtcaacgg ttacaccgaa acgcaggccg
    26641 acagtggcgc ggtgatgtat gcgctttatt tctcctgcga ggaaatgatc gcaccgctga
    26701 ctgatatcag ctcgctggat gatttcctgc gccattacga aaccttcacc gaaccggaag
    26761 gcacaccgga atttaaggcg cacatcagcc tgccaggtgc aaccgccgcc ggtgaaaaag
    26821 ccggaccgtc agaggaataa cagatgacaa ccatttttat caaacccgcg ccgggatgcc
    26881 tgatccgcga tccggacacc atgaaaccgc tggcgcagga gggagaggaa aaacccttca
    26941 ccccgttctg gtgccgccgt cttaatgacg gggacgtcat tgtggtggaa aaagcggctg
    27001 aagcagcacc ggcagcagcg tctgcaacga ctgatgccgc cggaaaaccc gcgggagcgg
    27061 cagcagaaaa acctgcgact gaaaaacccg cgactgaaaa acccgcagca tcagacaagg
    27121 ggacgtcctg atgattaact ttgattatat cggcgataac aatcgcattc cgctggtaca
    27181 gattgaaatc aataattcaa tggccgtgac cggcactcca ccacagcgtc aggcggtact
    27241 gttgtttggt caggctgcaa tgaaagacac caccattcag gggcgcggtc agcttgatgt
    27301 accagtacgt attacccgag cttcacaagc aagagaactc tgggggcgag gttccatgat
    27361 tgcactgatg gtagaggaat ttatcgccat taaccctgac acagagttat atgccattgc
    27421 acagggggtt ggaacaggtc agtccagggc atgcgtgatg aatatcattg gaacagcatt
    27481 ggcagacggg gtgttaagtg tttacatcgg cggtcgccgt tacctcctgc ccgtcagtaa
    27541 ggggaaaaaa ggtaaagagc ttgtggaaga gctggctaag gtcattaacg ctgacactga
    27601 tgcgccattc actgctatcg cagctgaagt cagtggtgat aatgctgaag gacttaaagg
    27661 gagcatgggg gttaacgccc gctttatcgg tgaatgctca gctcatgatt tacgggtgaa
    27721 ctattatgac ggtgaaacta caccggccgg tcttacgctg gagctcgtcg cgccaacaca
    27781 gaaggcaaca aaccccgata tcacccgtag cgtctcaggg atggggaatc gtcagtacaa
    27841 ctacatcgtc atgccctata aggatgcggc aaatctcaaa attctggcgg atgaactgct
    27901 gaaacgctgg ggaccggtaa aaatgtcgga cggtatggtc tggatggcgc ataccggcac
    27961 cttcggtacg gcaaccagtt ttggtgaatc ccgtaatgac ttcctgttta cctgcacttc
    28021 tattcctaaa gcaccagagc cggattatgt ctgggcggcc tccatttgtg cggtctgtgc
    28081 gccctccctg tcaacggatc cggcccgacc attacagaca ctggcgctgc ctgcccgtat
    28141 ggcacctgaa gcaccggctc gtctgacgcg ggaagagcga aacagcctgc tttataccgg
    28201 catgtccacg gtgaccgttg cggccgctga tgtggtgcag attgaacgtc aggtgacgat
    28261 gtaccgtcag aatgcatacg gcgaaagcga tcccagctat ctggatgtag aaaccatcta
    28321 caccctgtca tatctgcgtt attccctgcg cacctttatc acgcagcgtt tcccgcgcca
    28381 caaactggcc gataacggta ctcccgtccg gcccggtcag aatatcgtga cgccggagat
    28441 tatgacgctc cagctgattg cactggggga agaatgggtg gaactcgggc tggtcgaaaa
    28501 tctggacacc tttaaaaaga atctgctcgt ggagcgcaac acaaaagacc gtaaccgtct
    28561 ggatgtgatg tgtacaccgg atctggttaa ccagttccgc ttcatggccg cgcagatccg
    28621 tttcattttg tgaggtaagg catgagcgga aaacagcatc agggcacggc caccatccgt
    28681 gtgaacggac aggagtacgc cacccttgag ggagccacgt tctccccgtc cggctttgag
    28741 cgtgaagtgg tgaaaggcgc gaaagtctat ggctatcgtg agaaaccccg tgaggcgacg
    28801 ctggactgta aatttccggc tggcggagaa ggttcaccgg ctgccgatga aatcaacacc
    28861 tggactgcgg tcacgattga gttcgtggcc gataccggtg aagtccatat gatgacgaag
    28921 gcctggagca gtgaaccggc ctcgcttgac ggtggcggtg atatctccgc gaagttcgcc
    28981 agcgccacca gtacccgtgt ccagtaatca ggaaaaatgc aatgaccaca cgtaagaaaa
    29041 aaacggcggt ttctgacgag gccgtgatgg aggctatccg ggatgtactt gaagacggcg
    29101 atccccgcac cgccgggctg gctgaacagc tggcgaacgg ctatgtggat ctgctcgacg
    29161 gtctgccgtt cggggaatcc cgtgaatacc gcgtcacctt ccgcgaactg acggcgaaag
    29221 acagcattga cgcggaaacc gaggccgagc gtcttatgga aacccgaaat ggcggcccgg
    29281 ttctgatagc ctcgccggca ctgcgcggca ttgcactgct gcgccgtcag attgccgctg
    29341 tcggccagat tgaggggcca ttgtctccgc gtcagatcgg acagctcagc gagcgcgatc
    29401 tctcccgtct gatggcggcg gtaagcctgc ttgattccgc gatggcagga aagctggtcg
    29461 ctgaacgggg gcgaccagat gcagtgccgg gatcagattg aagaagccgc aattgtgctg
    29521 gggatggtga caaaaagcgg cccggagtgg gcgcttaacc tccccttatc gcagctttac
    29581 cggcactgcc gacagaccga aaaaattctc agaacgaagc agtaacccat ggcaaaaaac
    29641 ctgaaagcct ccctgattgt cgatttactc ggcaatattt ctgccaaatc ccgccagtgg
    29701 tcacaggagc tgggggcatt ctcccgttcc ggcaggacgg ggctgggtgg tcttggaaat
    29761 gcagcacgtc gtgccggtca ggagacagaa cttgtcggct cccgtatgca gcgaacgctt
    29821 gccggtgttc gtggcagtat ccgcacggtg acgtcagatt ttgaccgcct tcagggcagc
    29881 attaccggca ccatcgggcg gataagcaac ctttacggga tgctggcggg aggggctgcg
    29941 gtctacgggt ttaataaagc ctttatccgc ccggccgcag agatggagaa ctatattctg
    30001 cgtctcaacg ccattaatca tggcgataag gcaaaaacgg aagcagtgaa agcctgggcg
    30061 gtacagaacg ccaaagacac cacctggggg ctggcggggg ttatgcagga atacgcctcc
    30121 agtcgcgggt ttggcatgag tgacagggaa gcccgtcgct ttattaccat gcttcaggat
    30181 cagggcggct atcacggctg gtcactgtca gaggcacagg gtgcgtccct gcaactcaaa
    30241 cagatgtttg cccgccagag tatccaggca gcggacgcca atattctgac cggatacggg
    30301 attaacgttt accagttact ggccgataag ctgggtgtaa accagaagat catccgtgag
    30361 aaaggcgaga aaggaaaact gggaccggac agcattcgcc tgttatttca ggtgatggcc
    30421 gaacaggcaa aaggtgccca gaaaaacgcc atgaactcat ggacgggaat gacgtccatg
    30481 atgggtgacg tctgggatca gtttgcccgt gacgtgatgg cgaagggacc gtttgacagc
    30541 ctgaaaaaga gcctgaaagg ctttctggat tatgccgatg cggcacagaa aagcggctta
    30601 caggataagc tggccacaca gacggcctca gcactgaatc agggatttga gtatgccaga
    30661 gatgctgcga ccggctttta ccgggccatt cagaaagtca gggaaacgct tcaggcactg
    30721 cgcgacgccg gttatggcga tgcgctggat cgtattggtc agggtgcgca gaccgccgca
    30781 aagtacctga tgtatatgta tctcgcctcc cgtgcgctga aggtgctcag gtttgcaggg
    30841 acgggggcat tacgtctcgg cgcaaccccc ttacgctatg gcatggcgat gacctccgtg
    30901 ctgacgtcgc ccttccgcaa accgcagacc actgtaccgg gaacgcagcc cggacgcgct
    30961 ggccggttcc tgaatttcct taccggggta aatccggctg ccgttcagcc cgtgctggtc
    31021 accaactggc ctgcgggtgg cctggcctca ggtggcgggg atgtggtggt cagcggtgac
    31081 ggcaaaaccg tgcgcggccg taaaaaacgc ggccccggac gtggacgggg tgtcacgacc
    31141 gtcgtgactg ccggtgagca actggcggag agtgccggta aacagggctt cttcggtcgt
    31201 atgatgagcc gtgccggtgg tctgctaacg gcggccggaa accgcatggg gctggggcgt
    31261 tttgccgggc tgttccgtgg tgccggtcgg ctgggtggtg gtgccctgtg ggccggtgcc
    31321 atggcggctc ctgtactgct ggacagcagc gccagtgcag ccgataaagc cggtgccgtg
    31381 ggttctcttg ccggaagtat cgccggtggc gcgctggggg cggctgccgg tccggtcggg
    31441 gttgccattg gttccacggt gggcagctat ctcggtgatt atctgggggg ctggctgacg
    31501 caggcctggc agaaactgcg tggcagcagt gacgaaaacg gcggacaggc caccgcgaaa
    31561 accgccgcac gggtggagct tgtggcccct gaaggctggc gggcgcgcag tattgatgtg
    31621 gatgataccg cacagcatgg cctggatgtg aacgtctgga acggagggaa ctatggcctc
    31681 tactgacacg ccggggcgtg gctcctttcg tggcgtaccg ttcctggttt atcaggagca
    31741 gcgggaacgg ggcggacgta acattgtgcg ccgtgaatac ccactgcgtg aaagcggcgg
    31801 tgcggatgat ctggggccaa aactcccgga gttcacattc acggttctgg tgaccggaga
    31861 cgatcttcag acacagcgaa gccgcctgcg tgatgcgctg cgtgcacccg gtgccggtga
    31921 actgatgcac ccggattacg gcacgttaaa cgtgctgata aacagctttg aaagccgtta
    31981 taacgccagc gagcagggaa cggttgagtt cacgatcaac gtgatccccg ccagtgacga
    32041 taccgcccct gcggtggcag aagatacggc agctgcgctg gagcagaaaa gcggctcagc
    32101 aatgaatcag ctgtttaaca cgctttcaga cggctggacg gtgatttcag acggtctgca
    32161 tgatgtacag gccatgaccg acaccatcag cgataagatt gatgcactgg agaatgccgt
    32221 ttcggggatg gggattgtgc aggatatcag tgcctttacg gccagtttta ctgccctgaa
    32281 aggcaatgcc gccgccctga ttaatgcgcc ccgtcgcatg gctgaatcgc tggccggaat
    32341 gtttgccgtg ctgaccgggc tgcccggcga tccttccctg tctctgaccg gaaaagccgg
    32401 aacgccgtcc ggcagcctga ccacaaaccg tgacagcatg gcagagcagg ccatgccgca
    32461 gctgtaccgc acactttcct ccctgcgtta caccctcagc gaacaggatg atccacagcg
    32521 cctgattggc ctgacgcctg cggcacagaa aaatatccgt ctgctgcgga gcgtgatgca
    32581 gagtgcaacc ctggtgtcgc aggcacagac gctcggaaaa ctgctggatc aggtcatccg
    32641 gcagaatacg acgcacgttc aggatactga ccgttcgggc ggactggcct ggctggaaag
    32701 ttctgctgat gtgcagcgta tcaaccggga tttgagcgat gccatggaac aacaggtgct
    32761 ggatctgtcc gcacagggct ataccgggac tgcccttgca ttacgtgatg cagggcttgc
    32821 gctgacagaa gatttaacca cacggagcgt gcatcttccc ggtgcttttc aggtcatggt
    32881 tcgcaccacc gaaccggcac tggtcacgct gtaccgtgcc accggcaaca gcagtcgctg
    32941 gcagtatttt gtccgcagaa acaatatccc cgatccggtt tttgttccgg gtggccgcag
    33001 cgtggaggtg atcagtgagc agcaggattg aactgtatat cggtggcagt attttttccg
    33061 gctggctgac ggtcagcgtc cgtcgctcgc tggagcatct ggccgggtcg tttgaactgg
    33121 ggctgatgct gcccggtgag cgcatcccct cagccctgcg aaccggtcag tccctgacgc
    33181 tcagaattaa cgggcagacg gtcatcagtg gctggctgga tcaggtcagt cagcggatca
    33241 gtgcaacccg tcatcagatc agcatcagcg gtcgggataa aaccggcgac ctggtggact
    33301 gtgccgccat ccatcccggc agccagtggc gcaaccggac gctggcacag attgccgcag
    33361 atttatgtgc gcccttcggg atagccgtgc gctggcaggt taacgacgac acggctgcac
    33421 gccccttcag ctcttttacg ctggaaaact cagaaaccgt ggcggatgcg ctgacccgtg
    33481 ctgcccggca tcgcggggtg ctggtgacca gtaatgctga cggcgatctg gtgttcaccc
    33541 aggcaggaag ccagcagacg gacagactgg tgctgggaga taacctgctt gatgccgatt
    33601 acaacacgga ctggcgagga cgatacagcg aataccgtgt ccggggacac gggcgcggtg
    33661 gtggcaaacg gggagacagc gaatccgccg cccgtctggc agcacctgtg ggcgtcatca
    33721 gtgatgagca gatcggccgc taccggtcga aaatcatcct cgccgatcaa cagacagaca
    33781 ccaccggtgc acggcagcgt gccctgcgtg aaatgcgccg tgcgattgcc cgttcagaac
    33841 ggttttctgc caccgtgcgt ggctggttcc gggatgatgg ccggttatgg gatgtcaatc
    33901 tgctgaccgg tgtttcagcc ctgcgtttcg gtatagaaca gactgaactg ctggtctgtc
    33961 aggtggagtt tttacttgat gaacataacg gggaagtcac ccggctggta ctggcaccgc
    34021 gtgacggctt tatcgttccg gcagagccgg acagtaaggg ccggggtggt tccggtgacg
    34081 atgttgatgc ctttattcgc cagcagatga aaaaacaggg gatcagcttt gatgaatgat
    34141 gaagtgttca gccgcctgat tgccccggta acgcgcggta ttcgcctgtt gtttggccgg
    34201 ggcgttctga ccggcacaca tgacgaactg aaaatgcaga atgtgcagct caccggcatg
    34261 gacggcgaaa cctttgatga tgtggagcgt ccccagcagt acgggcagat cagcgttccc
    34321 ctgccgggtg cggaaacctt ttttgcctgt ctgggcggac agcgggatca gactgtggtg
    34381 cttgtggtgg aagaccggcg cagccgcccg accggactca cagccggaga tacgggggtg
    34441 tatcaccatg aggggcaccg gatacggtta acaaaggatg gtcgcattat tgtgacatgc
    34501 aaaatgctgg agatttacgc cgatgagggg atgcgggtgg atacgccgga ggccaccttt
    34561 acgggcaatg tgacggtgga taaaaacctg catgttaagg gtaatttcgt gcttgatggt
    34621 acaggtaaat cagacgggct gttcacgatg tctgatgccg ttattgccgg gatcacctat
    34681 tccggccatg tgcatcagga taacggcaaa ggcagtaaga caggagcgcc ggagaatggc
    34741 tgatattgca attgtatggg atcaggggtg cggttcgctg caactgaacg gtgccgactt
    34801 gctgacggat gacagtctgc tgacggccgt tctgatttcg ctgtttactg accgccgggc
    34861 gctggcatcg gatgaaatcc ctgacggtac gcgcgaccgc cggggatggt ggggagacag
    34921 ttttcgcccg cgccccattg gctcccgtct gtggcttctc agccgggaaa aaacgctggc
    34981 ctcggtgata agccgtgccc gtgcttacgc ggatgaggcg ctgggctggc tcagtcagga
    35041 tggcgtggcg tcatccgtgg tctgtcatgc agaacgtgtg ggacacgcgc ggctggcgct
    35101 ttcggtgcgc ataaccctgc ctgatggttc agtgaggccc atgatttttt atgctgatct
    35161 taagggggag taatgcctta tcagccttta ccgctggcac agttaatcac gcagacacag
    35221 caggatatca gccagcgcct gcccggctcg cagccgggtg tgaatgaaac caccctgaat
    35281 gccattgctt acgctcaggc agggttatct gctcaggagc atgagcatct ggcctggatt
    35341 gcgcgtcaga tcatcccgac cgaagccgat gaagccgaac tgctgaaaca ctgtgcattc
    35401 tggggcgtca tgcgtaagcc tgcctctcgg ggggacgggc cggttcagct gatgctgacc
    35461 acggatgcgg ggatcaagga aggtgtgctt ctccagcgaa gtgatggcgt ggtttaccgt
    35521 atcaccacct ccctgaccgg taaggccggt acgctgaatg tcagtgtgga agccgaaagt
    35581 gccggtcgtg cggggaatgc cccggcaggg acaaaactga catttatcac accgcaggcg
    35641 gggatcaacc agacggccac ggtgaccgga acggggatca ccggtggtgc ggatgtggaa
    35701 accgtgccgg agctgctttc ccgtctggtt ttccgggtgc agaatccacc gtccggcggc
    35761 acgcagtatg attttgaacg ctgggcgcgt gaagtgccgg gcgtgacgcg ggcatggtgc
    35821 cgcccggaat ggccgcaggc gggcagcgtg ggggtaacgt ttgttcagga taataacccg
    35881 gatattttcc ccggtgacgg tgatgttcag cgggtggcgg attatatccg cagtcatgat
    35941 gatccggcga ccggccagcc tgtcggacag cctctggggc cgacggtgac cgtgtttaaa
    36001 ctgaccaata agccggtgcc cttcagcatc aggatcatcc cgaaaacacc ggagaatcag
    36061 gcggccgtaa aacaggcgct gaccgacctg ttgtataacg aatcccggcc cggcggtctg
    36121 gttctgccgt cgtctttctg gcgggcggtg gcaggggtga aaaatctgga ggattttgaa
    36181 gtgcgcagtc cgctgacgtc agttcaggcg ggagacagtg agctgctgac ggtaggagaa
    36241 atcacatggc tgtaaccctg accccgcatc agcgcgccct gttgcagtta ctgcctgacg
    36301 ggctggcatg ggacaagcgt ccgtcatccg tactggcatc attatgtctg ggcctcagtc
    36361 attcgacagc gcgtgtatcc tggacgggta aacagctgct tgccgagcgt ttccctgaca
    36421 cgtcgcgcct gctgctggaa gactgggagc gttatctcgg gcttcccgag tgtgatatgg
    36481 ccggagccac cctcacggag cgacagcgtt atgccggaaa taaataccgg atgaagccct
    36541 cgctgaaccg tgaattttat atccggtttg cggcggagtt tggttatcag attgacattc
    36601 agccctcacc ggattcgcag tgggtcagta ttgtcacgat taacagtgaa accggctacc
    36661 ggaatatgaa tgtgctggat gatattctca cgccgctgcg tatttatgaa ggcggtgcgc
    36721 tggaatgtat tctgaaccgt tataagcctg catggcagac gtttatttac gtttatgcaa
    36781 acagccatga agaggagact atttaatgtt tcatgttgat aataattccg gcgtggcgaa
    36841 tatgcctgcg ctggcaccgg cgcagagtaa taccaccacc tggtttaccg aaggtgacgg
    36901 acaaaaaggt atcagctgga ttggtcagga ctggctgaat attctccagg ccgaactgct
    36961 gaatattctg gctgaagcca gtattcagcc ggataaggcg cagttaaacc agcttacgct
    37021 gtccattaaa gccattatcg ctgcgaatgc cttttcccgg aaaaataacc tgaaagaaat
    37081 tgctgatgcc ggtgcggagg cccagcgtct ggcccgtggt tatcttggtc tggggacgct
    37141 tgccacaaaa aacagtcttg gtcccgctga cgttaatgcc ctggcgaagg atcagaatct
    37201 cgccgacctg gagaataagg gaaccgcccg taataatctg gatgtttaca gcaaaagcga
    37261 aggtgataac cgttacctgc gcagggagca gaacggcgca gacattccgg ataaaggggc
    37321 ttttatcgat aacgtcggtt tacgggaaac ggtaaacagg gcggcgaatg ccctgccatc
    37381 ggacggcacc gccgttgccg cgaacaggct ggcaaatgcc cacacaatta atggtgttcc
    37441 ctttgacggg acgcaggata tcactatctc atcaggaaca gtaaccgcca taagactggg
    37501 gtcggtgacc gcacatatgc ccggaacatg ggaaagctgg gatctgaatc tggggggcgg
    37561 caatgttctt accggaatta aagtacagga tgtcggtaag aatacggcag ataacgtcgg
    37621 cggcgtgtat taccggccgt tgcagtatct gttaaatgga gcatgggtga cggctgcaag
    37681 tatataatga tattcagaag gccttccggc cttctttatt actgtggctg ctcaggccat
    37741 ttaatggcgt taaattcagc ttctgtttta atggcgggta gttccatttt cttcacctga
    37801 ctgatgtatg ccatccattg ggtaagtgct gctctgtctt catcgctgat ggtgcccagt
    37861 tgtagctccg ttctccagtc attaattttt tcatatgcct gattaagata tgactggcgc
    37921 attgcttcca gttttcttgc gtagttaacc ggaatacggg agattatgcc attatcaaac
    37981 ttccagtttc ctgaaatatc cgtgccttca gggagttcat cgacctcaac cacagaaaac
    38041 cccgcaggat acagcgccga ggcgtcctgt gatattgagc agataacgcc gctgtcaggt
    38101 gttatgcaaa gtttatattt tttggtgaat aatggcaggg attcgtagaa atccttacca
    38161 tcttcactct ggaaatactg aacattatta ccatatggca tattttcagg ataataacgg
    38221 gttatatttt ttagttccat aaaatcctcc tggaaataaa tctgttttaa aatatattat
    38281 ttactgacta tttataactg agataccgta atccatgtac ctccgactaa tttttgcagg
    38341 ggtctgaatg ctataaaatc gtttgaagca tcaactttac tgtcaatctg caggcctgcc
    38401 ataacattac cgtacgcaga gtaatatgcc ccaccgtaca ttacctgcct gttctctgct
    38461 cccagcctga tatcctggat atatcgggaa tccgcatcct gctgtgttat accagaagtg
    38521 atgtttatgt cctgcgtccc gtcaaaggga accccattaa tatttcttgg ggtggcgagt
    38581 ctattcgcgg caacggcggt gccgtccgat ggcagagcat tcgccgccct gtttaccgtt
    38641 tcccgtaaac cgaggttttc aggaaagcca ggattaactc tgttttattc ctcatgcgac
    38701 aattccggct ttttataata aggaaatcgt atgctgattg ggtatatcag ggtgtcaaca
    38761 aatgaccaga atacggcgct acaaagaaat gcgctggaat gtgcaggatg tgagctgttt
    38821 ttcgaagaca gaataagcgg taagacaagg gagcgtcccg gacttaaacg cgcattaaag
    38881 cgactccggc caggggatac cctgctggtc tggaaactgg atcgccttgg acgcagtatg
    38941 cagcaccttg tccttttaac agaagaactc cggcaacgcg gcgttaactt ccgcagcatt
    39001 acggacaata ttgatacatc cactcccatg ggacgtttct ttttccatat aatgggcgcg
    39061 ctggcggaaa tggagcggga actcattatt gaacgtacac gcgcgggtct tgctgctgcc
    39121 cgtgctcagg ggtgtcatgg cggcagacct cagaaactga cagagaaaga gcgggagcaa
    39181 atcgcccgtt tgctggctaa tggcgagtcc cgtaaacaaa tcgcactgat atttgatatt
    39241 ggcctttcca ccctttatcg ttattttcct gcctcaccag ctccgtcacc aaaagtaccg
    39301 tgacgctttt ttgaatttcc gcaaaacaca tatttttcag cggtcgctat tctgactctg
    39361 tttaattcag aaacaggcag aaaagacgat gtcagactcc aagtggaaaa tgctctccgc
    39421 catgcccgga gagttttcag tcaaggttgc cggtggcacg gtggcattta ttgaaagccc
    39481 tttccgccct tcgggtaata aaggggggat caccttcgca gactgcgtga tccgcttcag
    39541 cacgaaagag ccattatggg taatgccggt atccggtaat cccagtgcag aaatcaccag
    39601 ttcaggcgtg actggcatca ttccaatcac atctgacgtg gccggaacgc tcacgccctc
    39661 cgactggaat gcaccggata acgccgggcc ttccggtagc acgaatggca gcagttcaga
    39721 gccggaatat tactttgttg tcattctcgc gggtcagtca aatggcatgg cctacggcga
    39781 ggggctgccg ttaccggatt cgtttgaccg cccggaccca cgcattaagc aactggcacg
    39841 ccgcagtacg gtaacgccca acggtacgtc gtgtacatac aacgacatca ttccggcaga
    39901 ccactgcctg catgatgttc aggatatgag cgggataaac cacccgaaag ctgatctggc
    39961 aaaaggccag tacgggactg tcggtcaggg gttgcatatt gccaaaaaac tgcttcccta
    40021 tatcccacag aatgccggga tacttctggt gccctgttgt cgtggcgggt cagcattcac
    40081 cactggagcc gacggctcat tcagtgaggc cagcggtgct tccgctgatt cttcacgctg
    40141 gggggccggt aaacctctgt atcaggacct ggtaagccgc accaaagtcg cgctggcgaa
    40201 aaacccgaaa aacaaacttc tggctgtggt ctggatgcag ggagaggcag accttgcctc
    40261 aggaagccag cagcataata gtttattcac ggccatggtt cagcagttca gaactgacct
    40321 ggctccgctt gctgcgcagt gcgtgagtgg aaatgccacc acggtgccgt ggatttgcgg
    40381 tgataccacg tattactgga aaaacgctgg caccgataaa tatgaggcag tatacggtgg
    40441 ctacaaaggc aaggaagcac agaaaatttt ctttgtaccg ttcctgacgg atgagaatgg
    40501 acagagcacg ccaacgaatg ctccggcgga agacccggat attgtggctg tcgggtatta
    40561 cggtgcagca tcccgaaccc agggcagttt tgtttcgaca cagcgtgaca gccatttcag
    40621 ctcatgggca cgcaggggcg tcatttcaga ccgtctggcc tcagctatta tgctccatgc
    40681 agggcgcacg gctgaactga tgcgcgggca gaccgtgaca ccaccggatg agaagccgtc
    40741 acctgataca ccatcaaaac cgtccacgcc acccgcagac accacgacga tgagtacgct
    40801 atttgcttac cgggcatctg agtctgaagg acggttgaca ccacagggtt gggctgccgg
    40861 aggtggcaag gctcaaatcg tggatgatgc cggagccagc gggggtaagg caatgaaact
    40921 gaccaaggaa acaggaagag ccccctggta ccttgagcat gatgctggta atggtgcgga
    40981 cctgctgggt aaaggtggtc ttgtcagttg tcgttttaaa ctcgatggcg cgcttacggc
    41041 taatcagtac gcactggcgc tgtactggcc ggtttctgcc ctgccgcaag gggttactct
    41101 ggaaggaaat gccggtcata acctgctggc atcgttctac gtacagagcg atgccacaga
    41161 ccttaacgtg atgtatcaca aaggaaacac ggcgcagaac acgaagctgg ggtcattcgg
    41221 cgcatttaat aacgaatggc atacgctggg cttccgtttt gccggtaaca acagtattga
    41281 ggtgacgccg gtcattgatg gtaaggacgg gacaccattc atgctgtcgc agtctccggt
    41341 cggcacgttt acggcagaca agttgcgcgt gaccgatatc actaaaaatg cgacttaccc
    41401 ggtactgatt gacagtatcg tggtggaagt gaataacgcg taagcaggat aaaaaaatgc
    41461 cgccggtaag ggaaaacaag aaaccggcgg caaaaagatc gttatatcca aagcaaaaaa
    41521 tgcagtgcac ctttttacct aacagatatt accggtgtca acaccatgtc aataattggg
    41581 agggataatg acatttttac agcttatttt attgtatttc tgcacggcag tatgtgcgtt
    41641 atatctggtg agtggtggtt ataaggccat cagaaattat gttcgccgca aaattgagga
    41701 cgcagcagct gaaaaactca gtaaaacagc gcaggtaact ccgctcccaa aagacccgac
    41761 ccagccctga ttgcagggct ttattcccgc ataaggagga tatacatgcc agaaattaaa
    41821 ggcaccgtca ctgaagacct ggtaaaacag gcactgtatt ccggtgaagt aaataagttg
    41881 ctgaaagcgc aggtccgcaa ggattttgag gcacaaatcg acacttacgt tgatgaagtg
    41941 ctggcaaaac ttattgggcc agcagttgcg gccagtgatg ctgataatga gccgaaaacc
    42001 gacgcgcagc cagagcaacc agacccggct cagcccggaa ctgacagtac catgatgtaa
    42061 caatacaaac cggtggtgtc ctctgccggt ttgacactaa tgaaaacatc tttgccgcta
    42121 tccagccata tgtaatcgct ggatagtatt tcgtattcag aaatatagct gcttatggag
    42181 cagtcaatcc tgcttcccca tccgggcagg caaatgaacc ggtatcatta gtccgattca
    42241 cgcttttgta gaatcgttcc cagaatgcta tacagctcat tagcctgttg ctccatcatt
    42301 gagacggagg ctatacggtg caaagtgatt tcaggaggtg gcacacctcc atcttcttgc
    42361 tgtggaggaa aaacataaga tttaataaaa aagttgaagc atatgaactc tcccgcactc
    42421 gaactgtatt tgcaggcatc tgaataatcg gaaaaataat cattatagat ttccgcattt
    42481 tcaatcattt ttcgatttct cgtcgttaca tgattgtcca tagcagcctc ttacgctggc
    42541 aattaaccaa ttgacttcta taaattagtt gatttgtttg ctttgcggta ttcaaatgta
    42601 caatcattca gcacttatgc gttacagcac cgtttaaaat tagtgatcaa actcataaat
    42661 tttcgctaat gacatttcgt gttatttgtt gtatcaatta aataaatcaa atcgatcgtt
    42721 tttgtcgatc gattgagtga taaagatatc aacaacaaag caggaggtta tgcggccatg
    42781 tatcgaaaca tacggtgccg acattgtaac aagctactgg cacgagccag cttcagttat
    42841 ctggaagtta aatgtccgcg ctgtaaaacc ctcaatcaaa tcacatctca gagcgccaca
    42901 gagcacccca catacacaag gaaatcctgt cgtggggaac agaaagcaag ttacatcccg
    42961 tatcatcagc acgcctgaac ttattcacta taacgacaat atcgtcggtt atggctcccg
    43021 tgagttacgg gttgagacaa taagttgctg gctggcccgg ctggtcgtcg tcaataaaca
    43081 ttacagccat cgtttcgtaa ataattccta ccttcatctg ggcatatttt cagaacggga
    43141 gctggttggc gtaatgcaat ggggttatgc ccttaatcca aacaatggtg cacgcgtcgt
    43201 aactggcacg cagaaccgcg aatatatgga gcttaaccgg ttatggctgc atgactgtat
    43261 gccgagaaac tccgaatcaa gggcaatcag ttacgcgctc aaactaatca gacaacttta
    43321 tccgcaggtg cagtgggttc agtcgtttgc agatgagcgt tgtggctgtc ttggtgtcgt
    43381 gtaccaggca agcaattttg attatgtagg cagtcatgaa acaacattct atgaacttga
    43441 tggcgagtgg tatcacgaga tttgcaagaa tgcaatcaaa cgaggaggac agcgaggtga
    43501 acatctgcgg actaatatcg acagagccag tgttcataaa ttccgtcagt ttcgttacat
    43561 ccggtttctg gacaagagag ccagaaaacg ccttaacaca aagctattca aagtccagcc
    43621 ttacccaaaa ccacaaacag ttaaaactgg tttgaaagag agtgaatgaa cgcttaaaaa
    43681 ctcattctaa aactcaaaag aaaagcgccg atttagtatc aaataagttg cgtatcggcg
    43741 tttttcagcg gtatcaaatg aaatgcaaat cggtatcaaa aaaatcgccg cgctataatt
    43801 tgtcgccgtc agtacagcat gtaccggaac tttaggtttt tttcgttgag cgtcgcgaat
    43861 agcttcactg tgtggacgtg ctgcatgaac agggcggctg tctaaaatca tcaaggtcta
    43921 aatacactat gaggttgatt aggggttggt acaagcccaa ctaaccgaat caactatgag
    43981 gatgattcct agaacatccg aatcaactac ctgtaaaaac aggacaatat caggtgaatg
    44041 actatgcaat ttaacggtgt ttttccaagt atctatgcaa tcctacacaa attctatgca
    44101 gtatcggtat agtctctttt tagcataact tattgaaata tataaagaat ctgtgaagtc
    44161 tcttttcagg gtaaactgta tcgtgttagt cgtccctctc cagacactac atatagtgct
    44221 atatacaggg ctatataacg atatatagat agttggtcac atggtgtaac tatcaagaaa
    44281 cttaacgatt tcctacgtga aagttagaaa gtgatggtta tagttaccct cctgaatctt
    44341 catagcgctc tattaaccag tctcataagg aagtcctttt gagacacttt caacgtaacg
    44401 gtagaatgca ccctcaagta gtctcaaaag gttgtttttg gattatgaga cagctaagct
    44461 tatgagacta acttttgaga cttctaaggg gaacattatg gcgatacttg gatattgcag
    44521 ggtgagtaca gatgaccaat ccatcactaa ccagcaaatg caaattgaag aggcagggta
    44581 caacattgct aaatggttcg ctgatgaagc tgtcagtggt tctgtcaaag cttctttaag
    44641 aaacggtttc agcagttt
//
LOCUS       DAJTVJ010000018        42785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000018 DAJTVJ010000000
VERSION     DAJTVJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.018"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..559)
                     /locus_tag="OO448_000981"
     CDS             complement(<1..559)
                     /locus_tag="OO448_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6336266.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="OO448_000982"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="OO448_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6336267.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OO448_000983"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="OO448_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6336268.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OO448_000984"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="OO448_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6336269.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="OO448_000985"
     CDS             complement(4001..6322)
                     /locus_tag="OO448_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6336270.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OO448_000986"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="OO448_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6336271.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="OO448_000987"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="OO448_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6336272.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="OO448_000988"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="OO448_000988"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6336273.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OO448_000989"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="OO448_000989"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6336274.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..11582
                     /locus_tag="OO448_000990"
     CDS             11268..11582
                     /locus_tag="OO448_000990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6336275.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11595..12968
                     /locus_tag="OO448_000991"
     CDS             11595..12968
                     /locus_tag="OO448_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6336276.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OO448_000992"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="OO448_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6336277.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OO448_000993"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="OO448_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6336278.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OO448_000994"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="OO448_000994"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6336279.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OO448_000995"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="OO448_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6336280.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="OO448_000996"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="OO448_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6336281.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OO448_000997"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="OO448_000997"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6336282.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="OO448_000998"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="OO448_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6336283.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="OO448_000999"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="OO448_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="OO448_001000"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="OO448_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6336284.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="OO448_001001"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="OO448_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6336285.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="OO448_001002"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="OO448_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6336286.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="OO448_001003"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="OO448_001003"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6336287.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="OO448_001004"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="OO448_001004"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6336288.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="OO448_001005"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="OO448_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6336289.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="OO448_001006"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="OO448_001006"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6336290.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="OO448_001007"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="OO448_001007"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6336291.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="OO448_001008"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="OO448_001008"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6336292.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="OO448_001009"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="OO448_001009"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6336293.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /locus_tag="OO448_001010"
     CDS             31280..32185
                     /locus_tag="OO448_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336294.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="OO448_001011"
     CDS             32202..32564
                     /locus_tag="OO448_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6336295.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="OO448_001012"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="OO448_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="OO448_001013"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="OO448_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="OO448_001014"
     CDS             35034..35912
                     /locus_tag="OO448_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6336296.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="OO448_001015"
     CDS             35923..36276
                     /locus_tag="OO448_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6336297.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="OO448_001016"
     CDS             36288..37592
                     /locus_tag="OO448_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6336298.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVMRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="OO448_001017"
     CDS             37604..38689
                     /locus_tag="OO448_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6336299.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OO448_001018"
     CDS             complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="OO448_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6336300.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OO448_001019"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="OO448_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6336301.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OO448_001020"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="OO448_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OO448_001021"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="OO448_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6336302.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>42785)
                     /gene="frlA"
                     /locus_tag="OO448_001022"
     CDS             complement(42378..>42785)
                     /gene="frlA"
                     /locus_tag="OO448_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6336303.1"
                     /translation="AKDNLFFKCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLL
                     GYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWA
                     PIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT         9913 a        10878 c        11727 g        10267 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcatgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg tagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgc
//
LOCUS       DAJTVJ010000019        41012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000019 DAJTVJ010000000
VERSION     DAJTVJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.019"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            217..1881
                     /gene="asnB"
                     /locus_tag="OO448_001023"
     CDS             217..1881
                     /gene="asnB"
                     /locus_tag="OO448_001023"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6336304.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2261..2337
                     /locus_tag="OO448_001024"
     tRNA            2261..2337
                     /locus_tag="OO448_001024"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2295..2297,aa:Met,seq:cat)
     gene            2346..2430
                     /locus_tag="OO448_001025"
     tRNA            2346..2430
                     /locus_tag="OO448_001025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2380..2382,aa:Leu,seq:tag)
     gene            2454..2528
                     /locus_tag="OO448_001026"
     tRNA            2454..2528
                     /locus_tag="OO448_001026"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2486..2488,aa:Gln,seq:ttg)
     gene            2563..2637
                     /locus_tag="OO448_001027"
     tRNA            2563..2637
                     /locus_tag="OO448_001027"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2595..2597,aa:Gln,seq:ttg)
     gene            2653..2729
                     /locus_tag="OO448_001028"
     tRNA            2653..2729
                     /locus_tag="OO448_001028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2687..2689,aa:Met,seq:cat)
     gene            2778..2852
                     /locus_tag="OO448_001029"
     tRNA            2778..2852
                     /locus_tag="OO448_001029"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2810..2812,aa:Gln,seq:ctg)
     gene            2889..2963
                     /locus_tag="OO448_001030"
     tRNA            2889..2963
                     /locus_tag="OO448_001030"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2921..2923,aa:Gln,seq:ctg)
     gene            complement(3117..4292)
                     /gene="ubiF"
                     /locus_tag="OO448_001031"
     CDS             complement(3117..4292)
                     /gene="ubiF"
                     /locus_tag="OO448_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6336305.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4438..5862
                     /gene="miaB"
                     /locus_tag="OO448_001032"
     CDS             4438..5862
                     /gene="miaB"
                     /locus_tag="OO448_001032"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6336306.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDMAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6015..7055
                     /locus_tag="OO448_001033"
     CDS             6015..7055
                     /locus_tag="OO448_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6336307.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7052..7519
                     /gene="ybeY"
                     /locus_tag="OO448_001034"
     CDS             7052..7519
                     /gene="ybeY"
                     /locus_tag="OO448_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6336308.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7609..8487
                     /gene="corC"
                     /locus_tag="OO448_001035"
     CDS             7609..8487
                     /gene="corC"
                     /locus_tag="OO448_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6336309.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8512..10050
                     /gene="lnt"
                     /locus_tag="OO448_001036"
     CDS             8512..10050
                     /gene="lnt"
                     /locus_tag="OO448_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6336310.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10128..12005)
                     /locus_tag="OO448_001037"
     CDS             complement(10128..12005)
                     /locus_tag="OO448_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR6336311.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12460..13368
                     /gene="gltI"
                     /locus_tag="OO448_001038"
     CDS             12460..13368
                     /gene="gltI"
                     /locus_tag="OO448_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6336312.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13538..14278
                     /gene="gltJ"
                     /locus_tag="OO448_001039"
     CDS             13538..14278
                     /gene="gltJ"
                     /locus_tag="OO448_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6336313.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14278..14952
                     /gene="gltK"
                     /locus_tag="OO448_001040"
     CDS             14278..14952
                     /gene="gltK"
                     /locus_tag="OO448_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6336314.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14952..15677
                     /gene="gltL"
                     /locus_tag="OO448_001041"
     CDS             14952..15677
                     /gene="gltL"
                     /locus_tag="OO448_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6336315.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15795..16730
                     /gene="rihA"
                     /locus_tag="OO448_001042"
     CDS             15795..16730
                     /gene="rihA"
                     /locus_tag="OO448_001042"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6336316.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16822..18492
                     /gene="hscC"
                     /locus_tag="OO448_001043"
     CDS             16822..18492
                     /gene="hscC"
                     /locus_tag="OO448_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6336317.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18552..20003)
                     /gene="djlC"
                     /locus_tag="OO448_001044"
     CDS             complement(18552..20003)
                     /gene="djlC"
                     /locus_tag="OO448_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6336318.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(20000..20707)
                     /locus_tag="OO448_001045"
     CDS             complement(20000..20707)
                     /locus_tag="OO448_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6336319.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20809..21363
                     /locus_tag="OO448_001046"
     CDS             20809..21363
                     /locus_tag="OO448_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6336320.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21373..22800)
                     /locus_tag="OO448_001047"
     CDS             complement(21373..22800)
                     /locus_tag="OO448_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6336321.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22797..23504)
                     /locus_tag="OO448_001048"
     CDS             complement(22797..23504)
                     /locus_tag="OO448_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6336322.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23668..24645
                     /locus_tag="OO448_001049"
     CDS             23668..24645
                     /locus_tag="OO448_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6336323.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24715..25197)
                     /locus_tag="OO448_001050"
     CDS             complement(24715..25197)
                     /locus_tag="OO448_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6336324.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25432..28014
                     /gene="leuS"
                     /locus_tag="OO448_001051"
     CDS             25432..28014
                     /gene="leuS"
                     /locus_tag="OO448_001051"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6336325.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28029..28610
                     /gene="lptE"
                     /locus_tag="OO448_001052"
     CDS             28029..28610
                     /gene="lptE"
                     /locus_tag="OO448_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6336326.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28610..29641
                     /gene="holA"
                     /locus_tag="OO448_001053"
     CDS             28610..29641
                     /gene="holA"
                     /locus_tag="OO448_001053"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6336327.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29643..30284
                     /gene="nadD"
                     /locus_tag="OO448_001054"
     CDS             29643..30284
                     /gene="nadD"
                     /locus_tag="OO448_001054"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6336328.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30308..30919
                     /locus_tag="OO448_001055"
                     /pseudo
     CDS             30308..30919
                     /locus_tag="OO448_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31179..31496
                     /gene="rsfS"
                     /locus_tag="OO448_001056"
                     /gene_synonym="rsfA"
     CDS             31179..31496
                     /gene="rsfS"
                     /locus_tag="OO448_001056"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6336329.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31500..31967
                     /gene="rlmH"
                     /locus_tag="OO448_001057"
     CDS             31500..31967
                     /gene="rlmH"
                     /locus_tag="OO448_001057"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6336330.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31998..33899
                     /gene="mrdA"
                     /locus_tag="OO448_001058"
     CDS             31998..33899
                     /gene="mrdA"
                     /locus_tag="OO448_001058"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6336331.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33902..35014
                     /gene="mrdB"
                     /locus_tag="OO448_001059"
                     /gene_synonym="rodA"
     CDS             33902..35014
                     /gene="mrdB"
                     /locus_tag="OO448_001059"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6336332.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35025..36113
                     /gene="rlpA"
                     /locus_tag="OO448_001060"
     CDS             35025..36113
                     /gene="rlpA"
                     /locus_tag="OO448_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6336333.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDSVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36252..37463
                     /gene="dacA"
                     /locus_tag="OO448_001061"
     CDS             36252..37463
                     /gene="dacA"
                     /locus_tag="OO448_001061"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6336334.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37573..37836
                     /locus_tag="OO448_001062"
     CDS             37573..37836
                     /locus_tag="OO448_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6336335.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37937..38578
                     /gene="lipB"
                     /locus_tag="OO448_001063"
     CDS             37937..38578
                     /gene="lipB"
                     /locus_tag="OO448_001063"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6336336.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38838..39791
                     /locus_tag="OO448_001064"
     CDS             38838..39791
                     /locus_tag="OO448_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6336337.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            40000..40965
                     /gene="lipA"
                     /locus_tag="OO448_001065"
     CDS             40000..40965
                     /gene="lipA"
                     /locus_tag="OO448_001065"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6336338.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10300 a        10449 c        10291 g         9972 t
ORIGIN      
        1 cccccgtaaa atataaccat tcaataaaac ccgctaaaaa ttatcgattc atcaataaat
       61 acatcctcac accagaccct ttttcaccaa tcgacaatca ccattacgtt ttttattttt
      121 tcaccgccaa atcgcttgcg cacagtgctg ctttgcggca tttttttaaa caagcaaaca
      181 caacaagcaa caaataccag gttaacggag aaggttatgt gttcaatttt tggcgtattc
      241 gatatcaaaa cagacgcagt tgagcagcgt aagaaagcac tcgagctgtc acgcctgatg
      301 cgtcatcgtg gcccggactg gtccggtatt tatgccagcg ataacgccat tctcgctcac
      361 gaacgtctgt caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa
      421 acccacgtac tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg cgctgaatat
      481 ggcgatcgtt atcagttcca gacagggtct gactgtgaag tgatcctcgc gctgtatcag
      541 gaaaaagggc cggaatttct cgacgacttg cagggcatgt ttgcctttgc cctgtacgac
      601 agcgaaaaag atgcctacct gattggtcgc gaccatctgg ggatcatccc actgtatatg
      661 gggtatgacg aacacggtca gctgtatgtg gcctcagaaa tgaaatcgct ggtgccagtt
      721 tgccgcacga ttaaagagtt cccggcgggg agctatttgt ggagccagga cggcgaaatc
      781 cgttcttatt atcatcgcga ctggttcgac tttgatgcag tgaaagataa cgtgaccgac
      841 aaaaacgagc tgcgtcaggc tctggaagat tccgttaaaa gccatctgat gtctgatgtg
      901 ccttacggtg tgctgctttc tggtggtctg gattcctcaa ttatttccgc tatcaccaag
      961 aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccccagtta
     1021 cactcctttg ctgtaggtct gccgggttca ccggatctta aagcagccca ggaagtggca
     1081 aaccatctgg gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc
     1141 atccgcgacg tgatttacca catcgaaact tatgatgtga ccactattcg cgcttcaaca
     1201 ccgatgtatt taatgtcgcg taagatcaag gcgatgggca ttaaaatggt gctgtccggt
     1261 gaaggttctg atgaagtgtt tggcggttat ctttacttcc ataaagcgcc caacgctaaa
     1321 gaactgcatg aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgc
     1381 gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa
     1441 ttccttgatg tggcgatgcg cattaacccg caggataaaa tgtgcggtaa cggcaaaatg
     1501 gaaaaacaca tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt ggcctggcgg
     1561 cagaaagagc agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg
     1621 gcggcgcagc aggtttctga tcagcaactg gaaactgccc gcttccgctt cccgtacaac
     1681 acgccaacct caaaagaagc gtatctgtac cgggagatct ttgaagaact gttcccgctt
     1741 ccgagcgccg ctgagtgcgt gccgggcggc ccgtccgtcg cgtgttcttc cgctaaagcg
     1801 attgagtggg atgaggcgtt caagaaaatg gacgatccgt ctggtcgtgc ggttggcgtt
     1861 caccagtcgg cgtataagta agaaatgcga aacagccccg gtgaaatccg gggctgtttc
     1921 agttattgag gtgcttgttg attctgatat gcagtaagga aatgttccgg gtttgccaca
     1981 tcactgtttg acaactcatc actgtaggcc agtatttcat agattaattt cccaccacca
     2041 ttgttgaatt ccgaatttca tcctgtatga caaaagaatg gctgtagggt ggggaaaaaa
     2101 cacaaagtaa acaataattg acgaatatag cgccacgctg ttcgcaacct aaccaaacag
     2161 tcactttcga gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct atacgcataa
     2221 tgcgccccgc aacgccgata aggtatcgcg aaaaaaagat ggctacgtag ctcagttggt
     2281 tagagcacat cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccatct
     2341 tttttgcggg agtggcgaaa ttggtagacg caccagattt aggttctggc gccgcaaggt
     2401 gtgcgagttc aagtctcgcc tcccgcacca ttcaccagaa agcgttgtac ggatggggta
     2461 tcgccaagcg gtaaggcacc ggtttttgat accggcattc cctggttcga atccaggtac
     2521 cccagccatc ttcttcgagt aagcggttca ccgaccggtt attggggtat cgccaagcgg
     2581 taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc ccagccatcg
     2641 aagaaacaat ctggctacgt agctcagttg gttagagcac atcactcata atgatggggt
     2701 cacaggttcg aatcccgtcg tagccaccaa attctgaatg tgtcgaatac gttcggcaaa
     2761 ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg caccggattc tgattccggc
     2821 attccgaggt tcgaatcctc gtaccccagc caatttattc aagacgctta catcgtaagt
     2881 gcaccagttg gggtatcgcc aagcggtaag gcaccggatt ctgattccgg cattccgagg
     2941 ttcgaatcct cgtaccccag ccacattaaa aaagctcgct tcggcgagct ttttgctttt
     3001 ctgcgttcat tcaatgtcga atgcgatgtt gacacgtctt atccttcaat gtcggatgcg
     3061 acgctgccgc gtcttatccg acctacggtt ggcacgcatc cggcaatgtt gtaaggctac
     3121 aaccctaacg catatttcag cgcctgacgt ttcaacacgc cagcacgctc tgccgccatt
     3181 aacccgagat tacgcacaaa acgcagcggc ggcagattat tactgaatcc ggcataaaac
     3241 agatccatac cactttgcat aatgaagtta tccgccatgc gccgcatctg gtaacgcttg
     3301 aggacaggat aactggccca cgcttcaccg tagctgcggg cattcaccag aacatcaatc
     3361 agggcatcga catcacgata accaagattc accccctgcc ccgccagcgg atggatggta
     3421 tgcgcggcat cgcccaccag cgcaagcccc ggctgcacat actgcaacgc atgtcggcgc
     3481 gtcagcggaa acgcaccagc ggcaagcggt gtaacgtaac ccagacgcga cgggaaatgc
     3541 ttcgcgattt ccgcctggaa ttgcgccata ttcatattct gcaactggcg aatacgcgcc
     3601 ggagagtcat accacaccag cgatgcccag ttatcaaaca acggcagaaa cgcacgcggt
     3661 ccgtccgggg taaattgctg ccaggtgctg tcgccgggat cgttctcaca ctgtacgcta
     3721 atcaacatgc acgactgcgc atactgccag gcatgaacgc caattcccgc catctgccgc
     3781 acctgagaat ttgcgccgtc ggcaccaatc accagtttcg cgcggatcgt ttcaccgccc
     3841 ttcagttcca gctcctgaag atcattatct cgatgtaatg caatcagcga gcctggcaca
     3901 cgtaacgtta ctttcggatg cgcttccagc gcctgccaca gcgcctgttg caggacggtg
     3961 ttttccacca tatagccaag caacggtagc ttaagttcag cggcgtcaaa caccacatgc
     4021 gccgtttccc actcccacgt ttccagtctg cggtaaggat ggcaacgcat agcctgtact
     4081 gcatcccaga cccctaaccc tttaagcaat gataccgatg cggcgctgat cgccgagatc
     4141 cgcacgtccg gttggctatc agcgacaaac ggtgcaggtt ctgcgtgctc gatcaccgtt
     4201 accgaaaatc cgtgctgtgc cagccccagc gccagcgcgc cgccgaccat tcctccgccg
     4261 acaatggcaa tttccgttgg ttgatttgtc atggtcaatc atcctgttag caatatgctt
     4321 taagtttaca ggatttttac ccccaagggc tgataacgct catactggtc acaacggcag
     4381 caaagcatta cactatacgc cctgcattcc tggctactat ttcgcaagag caagtcgatg
     4441 accaaaaaac tccatattaa aacctggggc tgtcagatga acgagtacga ttcatcgaag
     4501 atggccgatc tgctggatgc cacccacggc tatcaactga ccgacatggc ggaagaagcg
     4561 gatgtgctgc tgctgaacac ctgctcaatc cgcgagaagg ctcaggaaaa agtcttccat
     4621 cagttgggtc gctggaaact gttaaaagag aagaatccag acctgattat cggcgtcggt
     4681 ggctgcgtgg catcgcaaga aggcgagcac attcgccagc gcgcccacta tgtcgatatt
     4741 atttttgggc cgcaaacgct gcaccgtctg ccggagatga tcaactccgt gcgcggcgac
     4801 cgcagcccgg ttgtagatat cagcttcccg gaaatcgaga agtttgaccg tctaccggaa
     4861 ccgcgcgccg aagggccgac cgcgtttgtc tccatcatgg aaggctgcaa taaatattgc
     4921 acctactgcg tggtgcctta cacccgtggt gaagaggtaa gccgtccgtc tgacgatatt
     4981 ctgtttgaga ttgcccagct tgcggctcag ggcgtgcgtg aagtcaacct gctcggtcag
     5041 aacgtgaacg cctggcgtgg tgaaaactac gacggctcca ccggatcgtt tgccgatctg
     5101 ctgcgtctgg ttgctgcgat cgacgggatc gatcgtattc gctttaccac cagccatccg
     5161 atcgaattca ccgacgatat catcgaagtg tatcgcgaca cgccggagct ggtgagcttc
     5221 ctgcatctgc cagtacaaag cggttccgat cgcattctga acctgatggg ccgtactcac
     5281 acggcgctgg agtacaaagc gatcatccgt aaactgcgtg cggcgcgtcc ggatattcag
     5341 atcagttctg acttcattgt tggcttccct ggcgaaacca ccgaagactt cgagaaaacg
     5401 atgaagctga ttgccgacgt caatttcgac atgagctaca gctttatctt ctctgcacgt
     5461 ccgggtacac cagccgccga tatggttgat gatgttccgg aagaagagaa gaagcaacgt
     5521 ctgtatattc tgcaagaacg tatcaatcag caagcgatgg catggagccg ccggatgctc
     5581 ggcaccaccc agcgtattct ggtagaaggg acttcgcgta agagcatcat ggagctttcc
     5641 gggcgtaccg aaaataaccg cgtggtcaac ttcgagggca caccggatat gatcggtaaa
     5701 ttcgtcgatg tagaaattac cgacgtctac ccgaactctc tgcgcggtaa agtggtgcgt
     5761 actgaagatg aaatgggtct gcgcgtggca gaaacaccgg aatcggtgat tgcccgtacc
     5821 cgcaaagaaa acgaccttgg cgtgggttat tatcagccgt aattctcagg cccgccgttc
     5881 cggtgggcct tgttttttct ctctttatcc ccatctttat cgcaatgctt gcgccttttt
     5941 cctgtggcgt gaataatttc cttataggcc ggtgaatgtt cagcttccgg cggcatacag
     6001 ataagaggaa cggtttgaac atagacactc gcgaaatcac cctggagcca gcagacaacg
     6061 cgcgtctgtt gagcctgtgc ggcccgtttg atgacaacat caagcagctc gaacgccgtc
     6121 tcggcatcga gatcaatcgc cgcgacaacc actttaaact gaccggccgt ccgatttgcg
     6181 tcaccgctgc ggcagacatt ctgcgtagcc tgtatgtcga tacagccccg atgcgcggtc
     6241 agattcagga tatcgaaccg gaacagatcc accttgcgat taaagaagcg cgagtactgg
     6301 agcaaagtgc ggagagcgtg ccggagtacg gcaaagcggt caatatcaaa accaaacgcg
     6361 gcgtaattaa gccgcgcacg ccaaaccagg cgcagtacat cgccaatatt ctcgaccatg
     6421 acattacctt cggcgttggc ccggcgggta cgggtaaaac ctacctggca gtggctgcgg
     6481 cagttgatgc cctggagcgt caggagattc gccgtattct gctgactcgt ccggcggtcg
     6541 aagccggtga gaaactgggc ttcctgcctg gcgatttaag ccagaaagta gacccgtatc
     6601 tgcgcccact gtacgacgcg ctgtttgaaa tgctgggctt tgagaaagtt gagaaactga
     6661 ttgagcgcaa cgttattgaa gtcgcgccgc tggcctatat gcgtggtcgt acgctgaacg
     6721 acgcgtttat cattctcgat gagagccaga acaccaccat cgaacagatg aagatgttcc
     6781 tgacccgtat cggttttaac tcaaaagcgg ttatcaccgg cgacgtcaca cagatcgacc
     6841 tgccgcgtaa tactaaatca ggcttacgtc acgccatcga agtgctggcc gatgtcgaag
     6901 agatcagctt taacttcttc cacagcgaag acgtggttcg tcaccctgtg gtggcgcgta
     6961 tcgttaacgc ctatgaagcc tgggaagaag ccgaacaaaa acgaaaagcg gcgctggcgg
     7021 cagaacgcaa gcgcgaagaa caggaacaaa aatgagtcag gtgatcctcg atttacaact
     7081 ggcatgtgaa gataattccg ggttaccgga agagagccag tttcaaacat ggctgaatgc
     7141 ggtgatcccg cagtttcagg aagaatcgga agtgactatt cgcgtggtcg ataccgccga
     7201 aagccacagt ctgaatctga cctatcgcgg taaggataag ccgaccaacg tgctctcctt
     7261 cccgtttgaa gtgccgcctg gcatggaaat gtcgctactg ggcgatctgg ttatctgccg
     7321 tcaggtggtt gagaaggaag ctcaggagca aggcaaacca ctggaggcgc actgggcgca
     7381 tatggtggtg cacggcagtc tgcatttgtt aggttacgat cacatcgaag atgacgaagc
     7441 agaagaaatg gaagccctcg aaacagagat tatgcttgct ctgggctatg aggatccgta
     7501 cattgccgag aaagaataat ttgccaggca tttgactggc agacgtcccc gccgttggtg
     7561 attaacgacg acgttttgat taactaacat gagaacccat acgacgccat gagcgacgac
     7621 aattcacaca gtagtgacac gataagcaac aagaagggat ttttctccct gttactcagc
     7681 caacttttcc acggtgaacc gaaaaaccgt gacgaactgc tggcgctgat ccgtgattcc
     7741 gggcagaacg accttatcga cgaagatacg cgcgatatgc tcgaaggggt gatggacatc
     7801 gcagaccaac gcgttcgcga catcatgatc ccccgctccc agatgattac cctgaaacgc
     7861 aaccagacgc tggacgaatg ccttgatgtc atcatcgagt ccgcccactc acgtttcccg
     7921 gtgattagcg aagacaaaga tcacattgaa gggattctga tggcgaaaga cttgctgccg
     7981 tttatgcgca gcgatgctga agccttcagc atggacaaag tgttacgtca ggcggttgtc
     8041 gttcctgaaa gtaagcgcgt agaccggatg ctgaaagagt ttcgctctca gcgttaccac
     8101 atggcgatcg tcattgacga attcggtggg gtttccggtc tggtgaccat tgaagacatc
     8161 ctggaactga ttgttggtga gattgaagac gagtatgacg aagaagatga tatcgacttc
     8221 cgtcagctga gtcgtcatac ctggaccgtg cgtgcactgg cttccattga agacttcaac
     8281 gaagcgttcg gcacccactt tagcgatgaa gaggttgaca ctatcggtgg tctggtgatg
     8341 caggcatttg ggcatcttcc ggcgcgtggc gaaactatcg acatcgacgg ttaccagttc
     8401 aaagtggcga tggccgacag tcggcgtatt attcaggttc atgtcaaaat cccggatgac
     8461 tcaccccagc cgaagctgga tgaataaaac cgaaactgga tagataacta catggctttt
     8521 gcctcattaa ttgaacgcca gcgcattcgc ctgctgctgg cgttattatt cggtgcctgc
     8581 ggaacgctgg ccttctctcc ttacgacgtc tggcctgcgg cgattatttc gttgatgggg
     8641 cttcaggcgt tgacctttaa ccgccgtcca ctccagtctg ccgctattgg cttttgctgg
     8701 ggatttggcc tctttggcag cggtattaac tgggtctatg tcagcatcgc gacctttggc
     8761 ggaatgcctg gcccggttaa catcttcctg gtggtgctgc tggcggcgta tttgtcgctg
     8821 tataccggac tgtttgctgg cgtgctgtcg cgtctgtggc cgaaaaccac ctggctgcgc
     8881 gtggcgattg ccgcccctgc tctctggcaa gtgaccgagt ttctgcgcgg ttgggtactg
     8941 acaggcttcc cgtggttaca gttcggctat agccagattg atggcccgtt aaaagggctg
     9001 gcaccgataa tgggcgtgga agccattaac ttcctgctga tgatggttag tggcctgctg
     9061 gcactggcgt tggtcaaacg caactggcgt ccgctggtgg tggccgtcgt gctgtttgcc
     9121 ctacccttcc cgctgcgtta catccaatgg tttaccccgc aaccggagaa aaccattcag
     9181 gtttcgatgg ttcagggcga tattccgcaa tcactgaaat gggatggaga tcagcttctt
     9241 aatacgctga agatttacta caacgcaacg gctccgctga tgggcaaatc atcgttgatt
     9301 atctggccgg agtcggcgat aaccgatctg gaaattaatc agcaaccgtt cctcaaagca
     9361 ctggacggtg agttgcgcga taaaggtagc tcgctggtaa ccgggattgt cgacgcgcgt
     9421 ctcaataagc agaaccgcta cgatacctac aacaccatca tcacactggg taaaggtgca
     9481 ccatacagct acgaatcagc cgatcgctat aacaaaaacc atctagtgcc gtttggcgag
     9541 tttgtcccgc tggagtcgat tctgcgtccg ttagcaccgt tctttgatct gccgatgtcg
     9601 tcgttcagcc gtgggccata tatccagccg ccgctgtcgg taaatggtat tgagcttact
     9661 gcggctattt gctacgagat cattctcggc gagcaagtgc gcgataactt ccgcccggat
     9721 accgactatc tgctgactat ctccaacgat gcgtggtttg gtaagtctat tggtccatgg
     9781 cagcacttcc agatggcgcg aatgcgtgcg ctggagctgg cgcgcccact gttgcgcagc
     9841 accaacaacg gcattacggc ggtgattggc ccgcaggggg aggttcaggc gatgatcccg
     9901 cagttcaccc gcgaggtgtt gaccactaat gtgacaccga ccaccggact cacaccgtat
     9961 gcacgtaccg gcaactggcc actgtgggtg ctgacggcgc tgtttggttt tgccgctgtg
    10021 ttgatgagtc tgcgagcacg taaacgttga tgtgcctgat gtatcctgca tgagcaaaac
    10081 gtactttctc attgagcttt ggggcatggt tatcatgccc ctttttctta cggttcaggg
    10141 atacaagcca cattgccata ttggggatga cgaataattg cctgcggatc aagcgagcgc
    10201 gtcctgtcca tttggatcaa cccgttcttc gccatgacaa atatttttct accatccgct
    10261 gaaaaacgaa ggtgattaac gtagctggca ggataaacgc caataatctg cccggcatta
    10321 atattgagca ggatgacata accttgcagg ccatatgaca ccaacgctaa atattgaccg
    10381 tccgggctaa taccctcaac ggaataaacg ctcctgctac accattgcgt cataatgctg
    10441 gcttgttgat cccctacgct tatacgtgaa atcccctgac tatcggcaaa aataacctcg
    10501 ccgttttcag gtcgaaaacg catcgccgat atcgttccac gcgcatcacc tgcgggaaag
    10561 tcttctttct gatgcgtgat ggtattgatc cgcacgatgt tacctctgcc tgctcccgga
    10621 accagtaatg ttctttcatc cggggaaagt gccataaccc aggcgcctgg tttaccgctc
    10681 gtccccgtcc cgtcgtattt ctccagcggg atatccgcca catgctgata agtcaccaga
    10741 tcaatcgctg gcacactatt actggtgcga ttcatcaccc aggcttttgt gccatcacgc
    10801 gagaggataa ggctgtcagg atgtgcaccg gtggtaatcg actgaataat ctcccctgtc
    10861 gccacgttaa tgaccgataa cgcattcgct tcaaatgaag tcgcataaat caacttttca
    10921 tccgggctta atgccagcga tctgacgccc tgatctttac agccaccaca atgtttgact
    10981 gacttttctt tcgctatggg cgctgtaaag gtatgcagga ttttcttttg tccgaggtcg
    11041 aaaacaacaa tgctgttcgt gttttcaatt gcggcataca acatattacc atcccgactg
    11101 atcgccatat catggatatc ggcaatctca gcgagcaaat caccactggc ctgttcacga
    11161 cttaccggag cggataatgt ttccctgagg gcgttgcgtt cctccgctaa ctgctgcttt
    11221 ttttgccgtc gctcttgttc aatctcctgt ggataaaacg cttcacgcag gctttgcctg
    11281 acctgtaaaa ctgactcatc catctgctgc tgcgcaagcc agatagcccc ggtcagaaga
    11341 agactgcccg caacaataat cccgccttcc gtaacgagtc ggttttgccc agataaacgc
    11401 gcgctcagcc aacccaacgc gccacccgca atcaggccgc caatgtggca agcgttatct
    11461 attcctgttt gccgggcacc gtacaacagt gtcagcgcca ccatacccaa cagaggaaat
    11521 actcgccgct ggtttttatg gtatgtgccc gtacctgaag caaactgtgt tgctatcgct
    11581 gcaccagcta ttcccagaat cgccccggat gccccaacgc tgatgactat ctgctcactt
    11641 tcgcgtaacg cataacaggc gctgatgagc ccaccaaaga cgccggagaa taaccaggta
    11701 atcaggagtc gccatttccc cagaatctgt tccgccacgc ctcccacgac caggaatgca
    11761 aacatattca tcagcagatg agaaacacta ctgtgcagaa agatactgct caataagcgc
    11821 cagggttcac ccgacaaagt aagaggcgca acgtttgcgc cgagatggat aagtgcggat
    11881 tgatcgacct cttcataact catccattgt gcgagataac aaaatatccc aatacagagt
    11941 gcaaaaagga taagggttat tgcgggtaat tgaacattta acggcttaac cgaagatgca
    12001 gacataaggt gtttcttcct tgaaaagaca ggtaaaaagc atttttcccg tgtgcagtta
    12061 tcatcccggc aaacgtccca tgccgaaaag ataattctgt ccgaaatatg actttttcag
    12121 cctaaaatta caatcgtaat taattcgccc ctaagtattt tttggcatgt aagttgcttt
    12181 atttactgtg taagcgatga aatcagccgc gacgcaacca ggttgcacca ccacaaatca
    12241 gcggtagcac caaaaaggtg cggtttaaga attttgcttc ggaatggtgc ggcgcatctc
    12301 gcaaaaacaa acaataaaac agcaatatct ttacatttag ccaaactaaa tgttacctaa
    12361 caggcacaac actgcacaat aaagttgcag acgataacaa cacaaacact cacaacgggt
    12421 atccatgcgt tcttaacgca gaagataaag gagttggata tgcaattacg taaacctgcc
    12481 acagcaatcc tcgccctggc gctttccgca ggactggcac aggcagatga cgccgccccg
    12541 gcagcaggca gtacgctgga caaaatcgcc aaaaacggtg tgattgtcgt cggtcaccgt
    12601 gaatcttcag tacctttctc ttattacgac aatcagcaaa aagtggtggg ttactcgcag
    12661 gattactcca acgccattgt tgaagcagtg aaaaagaaac tcaacaaacc ggacttgcag
    12721 gtaaaactga ttccgattac ctcacaaaac cgtattccac tgctgcaaaa cggcactttc
    12781 gattttgaat gtggttctac caccaacaac gtcgaacgcc aaaaacaggc ggctttctct
    12841 gatactattt tcgtggtcgg tacgcgcctg ctgaccaaaa agggcggcga tatcaaagat
    12901 tttgccgacc tgaaagacaa agccgtagtc gtcacttccg gcactacctc tgaagttttg
    12961 ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca tcatcagcgc caaagatcac
    13021 agtgactctt tccgcaccct ggaaagcggt cgtgccgttg cctttatgat ggatgatgcc
    13081 ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca actgggaaat cgtcggcaag
    13141 ccgcagtctc aggaagccta cggttgcatg ttgcgtaaag acgatccgca gttcaaaaag
    13201 ctgatggatg acaccaccgc gcaggtgcag acctccggtg aagcggaaaa atggtttgat
    13261 aaatggttca aaaatcccat tccgccgaaa aacctgaaca tgaatttcga actgtcagac
    13321 gaaatgaaag cactgttcaa agaaccgaat gacaaggcac tgaactaatt acaagaacca
    13381 ggggcggaaa ttccagccct ctcgattgtt acgtagcacg gacagactat acgcctgatg
    13441 gtcgttcccc atcgggcctg aaaaccgcaa tacgctgggt aacaatcttc gagggtagca
    13501 gttaacgctg ctaccctctt ttttctggag tagatttatg tctatagact ggaactgggg
    13561 tattttttta caacaagccc cgttcggcaa caccacctat cttggttgga tctggagtgg
    13621 ttttcaggtg acgatcgctt tgtcgatctg cgcctggatt atcgctttcc tcgtcggttc
    13681 atttttcggc attttacgta ccgttccaaa ccgttttctc tccggtctgg gtacgttgta
    13741 tgtcgaactg ttccgcaacg tgccgttgat tgtgcaattc tttacctggt atctggtgat
    13801 cccggagctg ttgccggaga aaatcggcat gtggtttaag gccgagctgg atcccaatat
    13861 tcagtttttc ctttcatcca tgctctgcct ggggctgttt accgccgccc gtgtttgcga
    13921 acaggtacgc gccgccattc agtcgttgcc gcgtgggcaa aaaaatgccg cgctggcgat
    13981 gggcctgacg ctaccgcagg cttatcgata cgtactgctg cctaatgctt atcgcgttat
    14041 cgtcccgccg atgacttccg agatgatgaa cctggtgaaa aactccgcca tcgcctcgac
    14101 tattggtctg gtggatatgg cggcgcaggc gggtaaattg cttgattact cagcccacgc
    14161 ctgggagtcg tttaccgcta ttacgctggc gtatgtgttg attaacgcgg ttatcatgct
    14221 ggtaatgacg ctggttgaac gtaaagttcg cctgcctggc aacatggggg gcaaataatg
    14281 tacgagtttg actggagttc cattgtccct tccctgccat atctgctcga cgggctggtg
    14341 atcacgctga aaatcaccgt cacggcagtc gtgattggta ttttgtgggg caccatgctg
    14401 gcggtgatgc gcttgtccag ctttgcgccc gtagcgtggt ttgccaaagc ctacgttaac
    14461 gtattccgct cgattccttt agttatggtt ttgctgtggt tttacctgat cgtgccaggt
    14521 tttctgcaaa acgtgctggg attatcacca aaaaatgata tccgtttaat ttcggcgatg
    14581 gtggcgtttt cgatgtttga agcggcctac tattcagaga ttatccgtgc tggtattcaa
    14641 agtatttctc gtggtcagtc gagcgccgcg ctggcgttgg gaatgactca ctggcagtcg
    14701 atgaaactga ttattctgcc gcaggcgttc cgcgcgatgg tgccgcttct gctcactcag
    14761 ggcatcgtac tgttccagga tacctcactg gtgtatgtgt tatctctggc cgatttcttc
    14821 cgtaccgcct caaccattgg tgagcgtgat ggtacgcagg ttgagatgat cctgtttgcc
    14881 ggatttgttt atttcgttat tagtcttagc gcgtcgttgt tggtcagcta cttgaaaaga
    14941 aggacagcat aatgattacc ctgaaaaatg tttcaaaatg gtatggtcac tttcaggtgc
    15001 tgaccgactg ctcaaccgaa gtgaaaaaag gcgaagtggt ggtggtttgc ggcccgtctg
    15061 gttccggcaa atcaacgctg attaaaaccg tcaacggcct cgaaccagtg cagcaaggtg
    15121 aaatcaccgt cgatggtatc gtggttaatg acaagaaaac cgatctggca aagctgcgtt
    15181 cccgcgtcgg gatggtattc cagcatttcg agctgttccc tcatctgtcg attatcgaaa
    15241 acctgaccct ggcgcaggtg aaagtgctta aacgcgataa agcgcccgct cgtgaaaaag
    15301 cattgaaatt gctggagcgc gtgggacttt ccgcacatgc caataaattt ccggcgcagc
    15361 tttccggcgg tcagcagcag cgtgtggcaa tcgctcgcgc attgtgtatg gatcctattg
    15421 cgatgctgtt cgacgaaccg acatcggcgc tggatccgga gatgatcaac gaagtgctgg
    15481 acgtgatggt ggaactggcg aacgaaggaa tgaccatgat ggtggtgacc cacgaaatgg
    15541 gctttgcccg caaagtggcg aatcgggtga tctttatgga cgagggtaaa attgtcgaag
    15601 actcgccgaa agacgctttc ttcgatgatc cgaaatcgga ccgcgcgaaa gacttcctcg
    15661 cgaaaatcct gcattaatca ctctggcgcg catacccaag tgcgcgccat ctcacgcttt
    15721 atcgtttcgc ccctcgtatc tgttacatcc tggcgttacc cttgtcgcaa agaagcacac
    15781 aacaaggagc aacaatggca ctgccaattc tgttagattg cgacccaggt catgacgacg
    15841 ctatcgcaat agttctcgcc ctcgcctcac cagagcttga tgtcaaagca attacgtctt
    15901 ccgccggaaa ccagacacca gaaaaaacct tacgcaatgt tctgcgtatg ctgaccttgc
    15961 ttaatcgcac cgatattccg gtagcaggcg gcgcggtaaa accgttaatg cgtgagttga
    16021 ttatcgcgga caatgtgcac ggcgaaagcg gtctggatgg cccggcatta ccggaaccga
    16081 cgttcgcacc gcaaaactgt acggcggtag agctgatggc gaaaacgctg cgcgaaagtg
    16141 cggaacctgt cacgattgtg tctaccggac cgcaaactaa cgttgccttg ctgctcaata
    16201 gccatccgga actgcatagc aaaattgccc gtatcgtgat tatgggcggc gcaatggggc
    16261 ttggtaactg gacgcctgcg gctgaattta atatttacgt tgacccggaa gcggcagaaa
    16321 ttgtcttcca gtcagggatc ccagtggtga tggccggtct ggatgttact cacaaagcac
    16381 aaatccacgt tgaagacacc gagcgtttcc gcgcgattgg caaccctgtt tcaaccattg
    16441 ttgccgaact gctggatttc ttcctcgaat atcataaaga cgaaaaatgg ggctttgtcg
    16501 gtgcaccgct gcatgaccca tgcaccatcg cctggctgtt gaaacccgag ttgtttacca
    16561 ctgttgagcg ctgggttggc gtggaaacac aggggaaata tagccagggt atgacggttg
    16621 ttgattatta ttatctgact ggcaataaac cgaatgccac tgtaatggtc gatgttgatc
    16681 gtcagggctt tgttgattta ctggctgatc gtctgaaatt ttacgcttaa cacacgtcat
    16741 tgattaagat caccacgcgt tacctgggtg gtcttaatct gtttatctgt ttatctcttt
    16801 ttctttcaag atataaatgc aatggataat gcagaactcg ccattggtat cgatctcggc
    16861 actaccaata gtttaattgc cgtctggaaa gacggtgccg cgcaattaat tccaaataag
    16921 ttcggtgaat atttaacacc atccataatt agcatggatg aaaataatca tattttagtc
    16981 ggaaaaccgg ctgtatcacg gcgtacttcg catccggata aaacggcagc gttatttaaa
    17041 cgtgcaatgg gcagtaatac caactggcgg ttaggcagcg acacatttaa cgcgccagaa
    17101 ctgtcctctt tggtattacg ctcattaaaa gaagatgccg aagaatttct gcaacgtccg
    17161 attaaagatg tggtgatctc cgttccggct tatttcagcg atgaacaacg caagcatacc
    17221 cgtttagcag cggagttagc cgggttaaat gcggtacgct taattaatga acccacagca
    17281 gctgcgatgg catatggcct gcatacgcaa caaaataccc gctcgctggt ttttgatctc
    17341 ggtggcggca cgtttgacgt cacggtgctc gaatacgcca cgccggtgat tgaagttcac
    17401 gcctccgctg gcgataactt cctcggtggc gaagatttta cccatatgtt ggtcgatgac
    17461 gttttaaagc gcgcggatgt cgccaggacc acgcttaacg agagtgaact ggcagccttg
    17521 tacgcctgtg tggaagcggc aaaatgtagc aatcaatcgc cattgcacat tcgctggcag
    17581 taccaggatg aaacgcggga gtgcgaattt tacgagaacg aactggaaga tttgtggttg
    17641 ccgctgctca atcgcttgcg agtgccgatt gaacaggcgt tgcgcgatgc acgtctgaag
    17701 ccgagtcaaa tcgacagtct ggtgctggtt ggcggcgcgt cacaaatgcc gctggtgcag
    17761 cgaatcgccg tgcgtctgtt tggcaaatta ccgtatcaaa gttacgaccc gagcaccatt
    17821 gtcgcgctgg gcgcagcaat ccaggccgcc tgccgcttac gcagtgaaga tattgaagag
    17881 gtgatcctca ctgatatttg cccttactcg ttgggcgttg aagttaaccg ccagggcgtt
    17941 tccggcattt tctcgccaat tattgaacga aacaccacag tacccgtgtc gcgtgtagaa
    18001 acttactcga ccatgcaccc ggaacaggat tctattacgg ttaacgtcta tcagggagaa
    18061 aaccacaaag ttaaaaataa cattctggtt gaatccttcg atgtgccgtt gaagaaaacc
    18121 ggggcttatc agtcgattga tattcgcttt agttatgata tcaacggctt gcttgaagtt
    18181 gacgtgcttc tggaagatgg cagcgttaag tccagagtca ttaatcatag cccggtaaca
    18241 ttgagcgcgc agcagattga agagagtcgg acgcggttat ccgcattgaa aatttatccg
    18301 cgcgatatgc taatcaaccg cacctttaag gccaaactgg aagagttgtg ggcgcgggcg
    18361 ctgggtgacg agcgagaaga gattggccgg gtgatcaccg attttgatgc ggcgttgcag
    18421 tcaaacgata tggcccgcgt cgatgaagtt cgtcgacggg cgagcgatta tttggctatt
    18481 gagatccctt aaacacggct gccggatgcg gcataaacgc tgcatccggc atcggcgcaa
    18541 aaaacacatc ctcagctaaa caacatcgcg atttttccga gcgcataacc tatcatcagc
    18601 accgttaagc tgattttgat aatactggta atgctctgtg gtttttcatc ctcatcattt
    18661 ttttcttcat ggttttcagg tgttggctca tcgacatctt catcctccgg ctcgagtttc
    18721 actttcgtca acagcatctg ggtgataatg gcctctaatg gatgagcatc tccaccttcc
    18781 agccttgtat taagacgggc cagcatctct tgtttcggga acgcttccag cacaacctgt
    18841 tccgctaccc gatacaaatc cagtaacggt gaatcttgct ctgtctcgct accgactctc
    18901 gatttccacc acgcccatgc tttgcgcttg aagaataacg actcaataaa cgattgacct
    18961 aacggcgata attttccgct atgtaaaatc tccgcccata acagccgcgt ccgggcgctc
    19021 tcaccaggaa catatttcaa cggtttacct tgcgcatcta ccatgcttcg cgcttcgata
    19081 accatcacta ataacgcgaa ataatccggg caatgacgct tgctccactg caacaacaaa
    19141 ttatcagctt gtgtagatgg gtaactttcc cagtacgcgc agagatcggc aagcaggctt
    19201 tccccctcgc cgcagtacaa acgttgtgca cactggtaat accgggcatc ttcgctttct
    19261 ggttcttccg cttgccactg tttggctaca ggaatgagtt ccgccatacc tgtttgcacc
    19321 gagctgtacc agcgcaacag tttgcgatgt aactttgcat catcaggaat gaaccacggt
    19381 ccttccatcg ccaaaaacgc agtgacatag tcaggatggt aacggcagca acgttccagc
    19441 gcgaagtaat attcaacagt ctggttctgt aacgcgacgg gatattccag aaggctaagg
    19501 aaatcgaaac aatcaccagc ctgaatggcc tcaagaaaag cctccctttc ttcctcatcc
    19561 attccttcgc tgtcattaaa cgaatgccag ttcaaacgct ctgccaacaa agaagcacaa
    19621 tttaatgaaa gatatcgcgc ttctatagcg attgcacaca gcggccagcg taattgatcg
    19681 acatcctcca tgttccaggt atttaattgc tgaataaatt tctgccaggc ggagggctga
    19741 aaacgatcac tttctgaatc cagtaatgtc ctgaatgcac gtagtatttc atgttcagcg
    19801 gtggcatctt tttcttcatc atcggcttcc tcgacaggat ttaccgccag tcgcagcgcc
    19861 tcttcatagg cctggcgtaa tgctttaaac ccctgcggat cggtttcggg atgacacaac
    19921 ggtaagcgag caagataagc ctggcggata atgtctattt gcgtcgtgct ttcaatttcg
    19981 aggatttgcc aacatgtttt cataggtcgc tcacatcctt aagcgttacc gggcattcag
    20041 gctggacagt taatggttgc cagggaagcg tggcatagaa acggtcgctt cccgctgcca
    20101 gctcttcgaa catccgactc ccggcgctat cgtatttgta cagggttagc gcctcgcgta
    20161 gcgaagcgtt gtcttcgcac tgagaagatt gccagtagag acgccctagt gaataggcag
    20221 aaaaatagtg catccagctg tgatagtaat ggtgagctcg tgcatagacg cgcgattgca
    20281 gccataaact ctcttcttcc gtcagccagt tgttgagcac accgacccgt gacagaaacc
    20341 ccattcgaac ataatcccag gctttgattc ccccttctcc acagcacata gcggtttggg
    20401 caacgaattg ggtataaatc aaccctctct ccgagccacc agcaattaat gctttcctct
    20461 cttcagggga agagcgttgc catgtcagat ataagccagc taaatcattg gcatgaccat
    20521 catcagtcat ctgataaata gtctgaatta aatcatcacg attatttatt ccccaggatg
    20581 atgagagatc gaattctttt atcccagtat acatttttgg cgagtcatgc gcgggtttgc
    20641 aactctgatt aagtatttcc atgggcgcag aaagcgcaaa aagaagatat tgttcttcct
    20701 tattcatttt ttaataatcc cttaagtgca ttaactgact acttttttat tattgatgag
    20761 tataaacttg acgctttcaa aataaaaaga aaatcgaagc attcacacat gaataaaaaa
    20821 tcaatgtata tattcgcaat ttttatagtt gcagcaatta cctgtattag ccaacccaag
    20881 aaaacgacgt tgcgtgataa agccatggtg aattatgcct ttgattattt aagatcacca
    20941 ggcagtctac cgttaaccac ggcagccacg gagctttccg cgattcatgg tcactcaaca
    21001 tcgcaatatc gccttggcga attttatctt catggtagcg acggtaaacc actggattat
    21061 acacaggcga gatactggta tgagcaatca gcagaacagg aaaatccgcg cgcacaaagc
    21121 aaactggggt ggatctacct gaaaggcctg ggagtcaaac ccgatacccg taaagcaatt
    21181 ctctggtata aggaagcagc tgaacaaggg tatgctcatg ctcaatatac tttgggtttg
    21241 atctacagaa atggcactgg tattaatgtt aaccattatg aatctcaaaa atggttaaaa
    21301 ctggccgcca aacaacatta caaaaatgcg gaaagattac ttgccggact tcccgcacat
    21361 taacgtcgac tgtcacccga agagatggag tatttttcct atcaaaccgg caaagataaa
    21421 aatgtagaaa ataattttga tgacgcccag acagcccagc ccgccatcgt caacgggcat
    21481 tggctgcggt tgcggctccg gctcgacggt ttcctgaccg gtaaaattcg ccgcctgaat
    21541 taataatcga cgaataatta atgcttccag tggacaacga tcttctgcat caaggcggct
    21601 cacaatcggc attaatgatt cttctgtcgg ttggctggcc tgcactaatt gtttggcaac
    21661 taaatataat ggccataagg gatgttctgt atcacgcgtg agtgagatcc gcggcttacg
    21721 tcctttgtgc aaaaccatct cgaccagtga acgccccagc gcagagagtt tttcatcgct
    21781 gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg ccggggacat aaagtagctg
    21841 tttaccctga tcgttaacca gttcacgagc ttcaatcatc atcaccacta acgggtaata
    21901 atcgacccga tgctgacggc accattgcaa tattaaagca tccgcctggg tggagggata
    21961 ctcgcgccag tagtcacaaa gttctgccag caagctttcg ccttctccac aatagacgcg
    22021 ttgcgcgtat tcataataac gcgcatcttc attatcaggt tcagcttccc gccacgaccg
    22081 ggcgtaatcc agaagttcgg ggatatccag gcgacctgcg ctataccagc ggagtaaatt
    22141 tctgtgcagt tttgcatcat cggggatgat cactgcgcga tgttgcgcta aatacaatgt
    22201 cagccattct ggatgcgatg accaaatccg agcgaacatt tggtaaaaat cgatggtgtc
    22261 attttgcacg gcaacgggca gatgcagaat ggtctggaag ttaaaaacat tccccttcgc
    22321 aatggcatat aaaaaggatt ccagttcttc ttcgtctatt tccccgacgt tttcctcctg
    22381 caaccaccgc aatctttctg ctaataacac cacgcactcg aaggataaat gggcagtgtt
    22441 catggcgatt gtgcacagcg accagcgtaa ttcatcaatc tcttccatcg agcaataatt
    22501 taattgctga atgaatcgct gccaggcgga gggcagaaaa cgttcgctat cagaggcaag
    22561 taacgcgcga aaggcgagca gaatttcatg ttctgctacc tcatattctt ctggttgcca
    22621 aacagattta gcaggcgact gcgcaatccg gagcgcctcc tcatacgcct gacgaagttg
    22681 tttaaaaccc tgcggatcgg tttctggatg aaaggacagt aacagcgcca gataagcgcg
    22741 gcggataata tcgacgtcag tcgtttcctc tatttcgagg atcttccagc aatttttcat
    22801 aggtcgctca tatccttaag cgtttccggg catgtggggt agtcagccag tggttgccag
    22861 gacaaggcag ggtaatacgc atcatcactg gcaaataatt gataaaacat atcattgccg
    22921 gaatcgtcgt attctttacg agctaagaat tcgcgtaata aaggcagatg atcatcttcg
    22981 ggagactgcc aatattgccg accaaaggta taaccggcaa agtattgccg ccagttacta
    23041 taataacgta gagcccgtag atgaatacgc gactggatcc atagactttc ttcttcactc
    23101 aaccagttat taagtacgcc cattcgactt aaaaaaccca tccggacata atcccatgct
    23161 ttaattcctc cctcaccaca gcattcggct gtgctggcga caaattgcgc ataagcctga
    23221 ccttgctcat ttagttcagc aagatagtca cgccattcac atggcgaata acgaaaccag
    23281 cggtgataaa accctgccaa ccgagcggca tgaccgttat cgatcattcg gtgaattgtg
    23341 cgcagcaggt catcacggtc atcaattccc catgaggaag aaaggtcaaa aaagcatgta
    23401 cctaaataca ttttggggga tgaatgcgaa ggtaaacagc attcattgcg tatttccatc
    23461 ggagtggaaa gtgcaaacaa tattttttgc gaccccatgt ccatataatt tatccttaaa
    23521 cattcattta atgaatgtaa actacatttc gagactcagt tatttatact tttctgacgc
    23581 aacataatac gcataaaata aggcttacgc aaaaatggaa cacataaaaa tataatatgt
    23641 tttatctaca accataagga aatgataatg gtttttacgt caagttgctg cgataattta
    23701 tcaatagatg agattatcga acgtgctgaa aaaggcgatt gcgaggctca atatattgtt
    23761 gggttttatt ataatcgcga tagcgcaatt gactctccgg acgacgaaaa ggccttttac
    23821 tggctgaagc tggccgctga gcaaggtcat tgtgaagcac agtattcctt agggcaaaag
    23881 tataccgagg ataaaagccg tcataaagat aatgagcaag ccatcttctg gctgaaaaaa
    23941 gctgccctac aaggccatac tttcgcttcc aacgcccttg gctggacact ggatcgtgga
    24001 gaagccccca attataaaga agcggttgtc tggtatcaga tagccgcgga gagcggaatg
    24061 tcttatgcgc aaaataatct tgggtggatg tacagaaatg gcaacggagt cgcaaaagac
    24121 tatgcgctgg catttttctg gtacaaacaa gctgcattac aaggccatag tgacgcgcaa
    24181 aacaatctgg ccgatcttta tgaagacgga aaaggcgttg ctcaaaacaa gacactcgcc
    24241 gcattctggt atttgaaaag cgcacagcag ggtaatcggc acgcccagtt tcaaattgca
    24301 tgggattata acgctggcga aggggtggac caggactata agcaagcgat gtactggtat
    24361 ctgaaggctg ccgctcagga aagcgtcggc gcttacgtca acatcggtta tatgtataaa
    24421 cacggacaag gcgttgagaa agattatcag gctgcctttg aatggtttac gaaagccgct
    24481 gaatgcaatg acgccactgc ctggtataac ctggccatta tgtatcacta cggagaagga
    24541 agacctgtcg atctccgaca ggctctcgac ctgtatcgta aagttcagtc atccggaacc
    24601 agagatgtca gtcaggaaat tcgtgagact gaagatttac tgtagagata aaaacaaggg
    24661 ccggggaatt tcattcggcc catattctct ttattgctgt gcgaaatcag actattacgg
    24721 ctcaaacggg cgtctctgga actggtcatg accacatttc gggcatagcg tcagcacttc
    24781 cggtgtgtag atcgggagat ggaagtgaca tttctcgcag accagatttc ccagcccgac
    24841 cacttctccg ctgtgataaa ccccatgatg attgaggtcc tggaaaactt cgcgccattc
    24901 aagctgcgtt ttatcggtga tatctgccag ctcctgccat aagctttctt taatcacccg
    24961 cataaagacg ctgtcagatt cttctttcag gctctcttca tagctcatgg cgaactcttc
    25021 caggtcacgt cttacagctc gcgtcagctc atcgacctcg gttcgcgtta actcccctgt
    25081 ttttattacg cgctcgcgcg cctgttccac cagtgcgtcg atatcacgtt cgccattgcg
    25141 caggcgttcg ctcagtgacg cgaccagttc acggtaatat tgagcaacct tgttcatcgt
    25201 ttcatctccc gggtcgttaa ctgactataa taatagacgt tctttgttcc ttgctttgct
    25261 aatacgccca caaactctgt aaattaatgt atgaataatc cgggcatcat ttcaacgtcg
    25321 agtgcgcgaa aggctgtttt gacgcggacg tttgggctat gctatgcgga tctgaaaaac
    25381 cacctcaacg ctacatttgt agccgtattg aaaacaggac cactggctgc catgcaagag
    25441 caataccgcc cggaagagat agaatccaaa gtacagcttc attgggatga gaagcgcaca
    25501 tttgaagtaa ccgaagacga gagcaaagag aagtattact gcctgtctat gcttccctat
    25561 ccttctggtc gactacacat gggccacgta cgtaactaca ccatcggtga cgtgatcgcc
    25621 cgctaccagc gtatgctggg caaaaacgtc ctgcagccga tcggctggga cgcgtttggt
    25681 ctgcctgcgg aaggcgcggc ggtgaaaaac aacaccgctc cggcaccgtg gacgtacgac
    25741 aacatcgcgt atatgaaaaa ccagctcaaa atgctgggct ttggttatga ctggagccgc
    25801 gagctggcaa cctgtacgcc ggaatactac cgttgggaac agaaattctt caccgagctg
    25861 tataaaaaag gcctggtata taagaagact tctgcggtca actggtgccc gaacgaccag
    25921 accgtactgg cgaacgaaca agttatcgac ggctgctgct ggcgctgcga taccaaagtt
    25981 gaacgtaaag agatcccgca gtggtttatc aaaatcactg cttacgctga cgagctgctc
    26041 aacgatctgg ataaactgga tcactggcca gacaccgtta aaaccatgca gcgtaactgg
    26101 atcggtcgtt ccgaaggcgt ggagatcacc ttcaacgtta acgactatga caacatgctg
    26161 accgtttaca ctacccgccc ggacaccttt atgggttgta cctacctggc ggtagctgcg
    26221 ggtcatccgc tggcgcagaa agcggcggaa aataatcctg aactggcggc ctttattgac
    26281 gaatgccgta acaccaaagt tgccgaagct gaaatggcga cgatggagaa aaaaggcgtc
    26341 gatactggct ttaaagctgt tcacccatta acgggcgaag aaattcccgt ttgggcagca
    26401 aacttcgtat tgatggagta cggcacgggc gcagttatgg cggttccggg tcacgaccag
    26461 cgcgactacg agtttgcctc taaatacggc ctgaacatca aaccagttat cctggctgct
    26521 gacggctctg agccagatct ttctcagcaa gccctgactg aaaaaggcgt gctgttcaac
    26581 tctggcgaat tcaacggtct tgaccatgaa gcggccttca acgccatcgc cgataaactg
    26641 actgcgatgg gcgttggcga gcgtaaagtg aactaccgcc tgcgcgactg gggtgtttct
    26701 cgtcagcgtt actggggcgc gccgattccg atggtgacgc tggaagacgg taccgtaatg
    26761 ccgaccccgg acgaccagct gccggtgatc ctgccggaag atgtggtgat ggacggcatt
    26821 accagcccga ttaaagcaga tccggagtgg gcaaaaacta ccgttaacgg tatgccagcg
    26881 ctgcgtgaaa ccgacacttt cgacaccttt atggagtcct cctggtacta tgcgcgctac
    26941 acttgcccag agtacaaaga aggtatgctg gattccgaag cggctaacta ctggctgccg
    27001 gtggatatct acattggtgg tatcgaacac gccattatgc acctgctcta cttccgcttc
    27061 ttccacaaac tgatgcgtga tgcaggcatg gtgaactctg acgaaccagc gaaacagttg
    27121 ctgtgtcagg gtatggtgct ggcagatgcc ttctactatg ttggcgaaaa cggcgaacgt
    27181 aactgggttt ccccggttga tgctatcgtt gaacgtgacg agaaaggccg tatcgtgaaa
    27241 gcgaaagatg cggcaggcca tgaactggtt tataccggca tgagcaaaat gtccaagtcg
    27301 aagaacaacg gtatcgaccc gcaggtgatg gttgaacgtt acggcgcgga caccgttcgt
    27361 ctgtttatga tgtttgcttc tccggctgat atgactctcg aatggcagga atccggtgtg
    27421 gaaggggcta accgcttcct gaaacgtgtc tggaaactgg tttacgagca cacagcaaaa
    27481 ggtgatgttg cggcactgaa cgttgatgcg ctgactgaag atcagaaagc gctgcgtcgc
    27541 gatgtgcata aaacgatcgc taaagtgacc gatgatatcg gccgtcgtca gaccttcaac
    27601 accgcaattg cggcgattat ggagctgatg aacaaactgg cgaaagcacc aaccgatggc
    27661 gagcaggatc gcgctctgat gcaggaagcg ctgctggccg ttgtccgtat gcttaacccg
    27721 ttcaccccgc acatctgctt cacgctgtgg caggaactga aaggcgaagg cgatatcgac
    27781 aacgcgccgt ggccggttgc tgacgaaaaa gcgatggtgg aagactccac gctggtcgtg
    27841 gtgcaggtta acggtaaagt ccgtgccaaa atcaccgttc tggtggacgc aacggaagaa
    27901 caggttcgcg aacgtgctgg ccaggaacat ctggtagcaa aatatcttga tggcgttacc
    27961 gtgcgtaaag tgatttacgt accaggtaaa ctcctcaatc tggtcgttgg ctaagcgcgg
    28021 gaggaagcgt gcgatatctg gcaacattgt tgttatctct ggcggtgtta atcaccgccg
    28081 ggtgtggctg gcatctgcgt gataccacgc aggttccttc cactatgaag gtcatgatcc
    28141 tggactcagg cgatccgaac gggccattaa gccgtgcagt gcgtaaccag ttacgtctga
    28201 atggtgtcga gttgcttgat aaagaaacca cgcgtaagga cgttccatcc ctgcgtttgg
    28261 gtaaagtgag catcgcgaaa gataccgcat cggtattccg taacggtcaa acagcagagt
    28321 atcagatgat catgacggtg aatgcgaccg tgttgatccc cggccgtgat atctacccga
    28381 ttagcgccaa agtcttccgt tcgttctttg ataacccgca aatggcgtta gcgaaagata
    28441 acgaacaaga catgatcgta aaagagatgt acgaccgtgc tgccgaacag ctgattcgta
    28501 agctgccaag catccgtgct gcggatattc gttccgacga agaacagacg tcgaccacaa
    28561 cggatactcc ggcaacgcct gcacgcgtct ccaccacgct gggtaactga tgattcggtt
    28621 gtacccggaa caactccgcg cgcagctcaa tgaagggctg cgcgcggcgt atcttttact
    28681 tggtaacgat cctctgttat tgcaggaaag ccaggacgct gttcgtcagg tagctgcggc
    28741 acaaggattc gaagaacacc acactttttc cattgatccc aacactgact ggaatgcgat
    28801 cttttcgtta tgccaggcta tgagtctgtt tgccagtcga caaacgctat tgctgttgtt
    28861 accagaaaac ggaccgaatg cggcgatcaa tgaacaactt ctcacactca ccggacttct
    28921 gcatgacgac ctgctgttga tcgtccgcgg taataaatta agcaaagcgc aagaaaatgc
    28981 cgcctggttt actgctcttg cgaatcgcag cgtgcaggtg acctgtcaga caccggagca
    29041 ggctcagctt ccccgctggg ttactgcgcg cgcaaaacag ctcaacttag aactggatga
    29101 cgcggcaaat caggtgctct gctactgtta tgaaggtaac ctgctggcgc tggctcaggc
    29161 actggagcgt ttatcgctgc tctggccaga cggcaaattg acattaccgc gcgttgaaca
    29221 ggcggtgaat gatgccgcgc atttcacccc ttttcattgg gttgatgctt tgttgatggg
    29281 aaaaagtaag cgcgcattgc atattcttca gcaactgcgt ctggaaggca gcgaaccggt
    29341 tattttgttg cgcacattac aacgtgaact gttgttactg gttaacctga aacgccagtc
    29401 tgcccatacg ccactgcgtg cgttgtttga taagcatcgg gtatggcaga accgccgggg
    29461 catgatgggc gaggcgttaa atcgcttaag tcagacgcag ttacgtcagg ccgtgcaact
    29521 cctgacacga acggaactca ccctcaaaca agattacggt cagtcagtgt gggcagagct
    29581 gaaagggtta tctcttctgt tgtgccataa acccctggcg gacgtattta tcgacggttg
    29641 atatgaaatc tttacaggct ctgtttggcg gcacctttga tccggtgcac tatggtcatc
    29701 taaaacccgt ggaaacgctg gcgaatttga ttggtctgac gcgggtcaca atcatcccta
    29761 ataatgttcc tccgcatcgt ccccagccgg aagcgaacag cgtgcagcgt aaacacatgc
    29821 ttgaactggc gattgccgac aagccattat ttactcttga tgaacgcgag ctaaagcgca
    29881 atgccccctc ttacactgcg caaacactga aagagtggcg gcaggaacaa ggaccggacg
    29941 tgccgctggc gtttattatt ggtcaggatt cactgctgac ctttccgacc tggtacgaat
    30001 acgaaacgat actcgacaat gcacatttga tcgtctgtcg gcgtccaggt tacccacttg
    30061 aaatggcgca accgcaatac cagcaatggc tggaagatca tttgacacat aacccggaag
    30121 atcttcacct tcagcctgcc ggtaaaattt atctggctga aacgccgtgg tttaacatct
    30181 cggcgaccat catccgcgaa cgtttgcaaa acggtgaatc gtgtgaggat ttattgccgg
    30241 aaccggtact gacttacatt aaccaacaag gcttgtatcg ctgatacctg tcgttacgga
    30301 gggcatgatg cgactgtggt taattcgtca tggtgaaacg caagcgaata tcgatggtct
    30361 ttacagcggt catgcgccca cccccctgac cgcgcgcggt attgagcaag cgcaaaatct
    30421 gcatacgctg ctacatggtg tttcctttga tctggtttta tgcagtgaac tggaacgggc
    30481 acagcatacc gcacgactgg ttctcagtga ccgccagctc cccgtgcaaa tcatccctga
    30541 actcaacgaa atgttttttg gcgactggga gatgcgacat catcgcgacc tcatgcaaga
    30601 agatgccgaa aactatagcg cgtggtgcaa tgactggcag catgcaatcc ccacgaacgg
    30661 tgaaggattt caggcatttt cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaatt
    30721 tcagcactat cagaatattt tagtcgtcag ccatcagggt gtactgagtc tgttaatcgc
    30781 ccgtttaatt ggcatgcctg ccgaggctat gtggcatttt cgcgttgact aggggtgttg
    30841 gagcgccatt gatatcaacc aaaaattcgc gacgctacgc gtcctcaata gccgtgccat
    30901 cggggtcgag aatgcatgac ttttctgttt ttttacgggt aagccgcaac gaccattgac
    30961 agacccgggc aggctgatat tctccgcagc cagacttttt tcgccagaca cgactttgta
    31021 gaaattgttt tacaaaaatg gcgatgcaat ctgcggcgcg gggtgggatg atagcccact
    31081 ttcgaaagcc gattcggcga caattgtccc gaaatcgcct ctggttcagg tatactgaca
    31141 gaccattttt atctatttga ttcacccagg gggaaaactt gcagggtaaa gcactccagg
    31201 attttgttat cgacaaaatt gatgacctca aaggtcagga catcatcgcc ttagacgttc
    31261 aaggcaaatc cagcatcacc gactgcatga tcatctgtac gggtacgtcc agccgtcatg
    31321 ttatgtccat tgctgaccac gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg
    31381 gcgtagaagg tgaaaacagc gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc
    31441 atgtcatgca ggaagagagc cgtcgcctgt atgaactgga aaaactctgg agttaatgcg
    31501 tgaagctgca acttgtcgcc gtgggaacga aaatgccgga ctgggtacaa accggtttta
    31561 ccgagtacct gcgtcgtttt ccgaaagata tgcccttcga gctgattgaa attccggccg
    31621 gaaaacgcgg caagaatgcg gacatcaagc gcatactcga caaagagggt gagcagatgt
    31681 tggcggccgc aggcaaaaac cgcattgtca ccctcgatat tccaggcaag ccctgggata
    31741 cgccgcagtt agccgctgag ctggaacgct ggaagctgga tggtcgcgac gtcagtctac
    31801 tgattggcgg gcctgaaggg ttgtcgcctg cctgtaaagc ggcggcggag cagagctggt
    31861 cgctgtcggc gcttaccctc ccccatccgc tggttcgcgt gctggtcgca gagagtctgt
    31921 accgggcgtg gagcatcacc accaaccatc cttatcaccg tgagtgataa gggagctttg
    31981 agtagaaaac gcagcggatg aaactacaga actcttttcg cgactatacg gcagagtccg
    32041 cgctgtttgt gcgccgggcg ctggtcgcct ttttggggat tttgctgctg accggcgtgc
    32101 ttatcgccaa cctgtataat ctgcaaattg ttcgctttac cgactaccag actcgctcta
    32161 atgaaaaccg cattaagctg gtgcctatcg cgcccagccg cggcattatc tacgaccgta
    32221 atggtatccc tctggccctc aaccgcacta tctaccagat agaaatgatg ccagagaaag
    32281 tcgataacgt gcagcaaacg ctggacgctt tgcgcagcgt ggttgatctg accgatgacg
    32341 atattgctgc attccgaaaa gagcgcgcac gttcacaccg tttcacctct attccggtga
    32401 aaactaacct gaccgaagta caagtagctc gctttgccgt caatcagtac cgttttccgg
    32461 gtgtcgaagt taaaggctat aaacgtcgtt actatcctta cggttcggcg ttgacccacg
    32521 tcatcggcta tgtgtcgaaa atcaacgata aagacgtcga acgcctgaat aatgacggca
    32581 aactggccaa ctatgcggca acgcatgata tcggtaagct gggcattgag cgttactatg
    32641 aagacgtgct gcacggtcag accggttatg aagaggttga agttaacaac cgtgggcgtg
    32701 ttatccgcca gttaaaagaa gtaccaccgc aagccggaca cgatatttac ctgacgctgg
    32761 atctcaaact ccagcaatat attgaaacgc tgctggcggg tagccgcgca gctgtggttg
    32821 tcaccgatcc gcgtacaggt ggggtgctgg cgctggtttc cacgcctagt tatgacccaa
    32881 acctgtttgt tgacggtatc tccagcaaag attattccgc cttgttgaac gccccgaata
    32941 caccgctggt gaaccgcgcc acacaggggg tttatcctcc cgcgtctaca gttaaaccct
    33001 atgtggcggt ttcggcattg agcgccgggg tgatcacgcg caatacgacg ctgtttgacc
    33061 caggctggtg gcaactgcca ggttcggaaa aacgttatcg tgactggaaa aaatggggcc
    33121 acgggcgtct gaatgtcaca agatcgctgg aagaatctgc ggataccttc ttctatcagg
    33181 tggcctacga tatggggatc gatcgcctct ccgaatggat gggtaaattc ggttatggtc
    33241 attacaccgg tatcgacctg gcggaagaac gttccggcaa catgcctacc cgcgaatgga
    33301 aacagaaacg ctttaaaaaa ccgtggtatc agggtgacac cattccggtt ggtatcggtc
    33361 agggttactg gacagcgacc ccaatccaga tgagtaaggc actgatgatc ctgattaatg
    33421 acggtatcgt gaaggttcct catttgctga tgagcaccgc cgaagacggc aaacaggtgc
    33481 catgggtaca gccgcatgaa ccgcccgtcg gcgatattca ttccggttac tgggagctgg
    33541 cgaaagacgg tatgtacggt gttgctaacc gccctaacgg tacggcgcat aaatactttg
    33601 ccagcgcacc gtacaaaatt gcggcgaaat ccggtaccgc tcaggtcttc ggtctgaaag
    33661 cgaacgaaac ctataatgcg cacaaaattg ccgagcgttt acgtgaccac aaactgatga
    33721 ccgcctttgc gccatacaac aatccgcaag tggctgtcgc catgattctg gagaacggtg
    33781 gtgcgggtcc ggcggttggt acactgatgc gccagatcct cgaccacatt atgctgggtg
    33841 ataacaacac cgatctgcct gcggaaaatc cagcggttgc cgcagcggag gaccattaat
    33901 catgacggat aatccgaata aaaaaacatt ctgggataaa gtccatctcg atcccacaat
    33961 gctgctgatc ttactggcat tgctggttta cagcgccctg gttatctgga gcgccagcgg
    34021 tcaggatatt ggcatgatgg agcgtaaaat cggccaaatc gcgatgggtc tagtcatcat
    34081 ggtggtgatg gcgcaaattc ctccacgcgt ttatgaaggc tgggccccct atctctatat
    34141 catctgtatt attttgctgg tggcggtaga tgctttcggt gccatctcta aaggtgctca
    34201 acgctggctg gacctcggta ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt
    34261 accactgatg gttgcgcgct ttatcaaccg cgacgtttgc ccgccatcgt tgaagaacac
    34321 tggcatcgcg ctggtgctga tatttatgcc cacgctgctg gtggctgcac agcctgacct
    34381 gggaacatca atcctcgttg cgctttccgg tctgtttgta ctgttcctct ctggccttag
    34441 ctggcgtctg attggcgtcg cagtagtgct ggtggcggcg ttcattccga ttctgtggtt
    34501 cttcctgatg catgattacc agcgccagcg cgtaatgatg ctcctagacc cggaatcaga
    34561 cccactcggc gcgggctatc acattattca gtctaaaatt gctattggct ccggcggatt
    34621 acgcggcaaa ggctggctgc acggcactca gtcacagctt gaatttctcc ccgaacgcca
    34681 taccgacttt atcttcgcgg tactggcgga agagctggga ttagtgggca ttctgattct
    34741 gctcgctctc tacattctgc tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac
    34801 cacctttggt cgcgtcatgg ctggtggctt aatgctgata ttattcgttt atgtcttcgt
    34861 aaatattggt atggtaagcg gtattctgcc ggttgtaggg gttccgctcc cactggtcag
    34921 ttatggagga tcggcgctaa ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca
    34981 cacccacagg aaaatgttgt cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc
    35041 tcgggatctg catcgcggca ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac
    35101 agacggtaag tgtaccgcag cctgcggtat gtaacggccc catagttgaa attagcgggg
    35161 cggacccgcg tttcgaacca ctgaacgcga cggcaaatca ggattaccag cgcgacggta
    35221 aaagctacaa aatcgtgcag gatccgtctc gatttagcca ggcgggactg gcggcaatct
    35281 atgatgccga acccggcagt aacctgacgg cctctggcga agctttcgat ccgacacagc
    35341 tgacggcggc ccatccaacg cttccgatcc ccagctacgc cagaatcact aacctggcta
    35401 acgggcgaat gatcgtggtg cgcattaatg atcgcggccc ttacggcaac gacagcgtta
    35461 tttcactttc tcgcgcggca gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta
    35521 tcgatccgat tattgtcgcc caggatggtt cgctttctgg tcctggtatg gcttgtacca
    35581 cagtcgccaa acagacttac gccctgccag cacctcccga tttaagcggt ggcgcgggaa
    35641 caagttcagt gtctggcccg cagggtgaca ttcttccggt cagtaattcg acgctaaaaa
    35701 gcgaagatcc gaccggcgcg ccggtaacca gtagcggttt cctcggcgct ccaacgacct
    35761 tagcgcctgg tgtactggaa ggcagcgaac cgacgcctgc gccacagccc gttgttacag
    35821 ctccgtcgac aacgcctgca acctcgcctg caatggtgac accgcaagcc gcctcgcaaa
    35881 gcgccagcgg caactttatg gtgcaagtcg gggccgtaag cgatcaggct cgtgcgcaac
    35941 agtaccaaca gcaactggga cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg
    36001 cggtctggcg gatccagctt ggcccattcg ccagcaaagc cgaagccagt accttgcagc
    36061 aacgtttgca aaccgaagcc caattacagt catttattac taccgcgcag tagcgtaaag
    36121 caggcatctg atgtgtcaat ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga
    36181 tgcctgccgg tagtgcattt gctatagtag ggcacttttt taattccatc acggatgtcg
    36241 tagttcagac catgaatacc attttttccg ctcgtatcat gaagcgcctg gcgctcacca
    36301 cggctctttg cacagccttt atctctgctg cacatgccga tgacctgaat atcaaaacta
    36361 tgatcccggg tgtaccgcag atcgatgcgg agtcctacat cctgattgac tataactccg
    36421 gcaaagtgct cgccgaacag aacgcagatg tccgccgcga tcctgccagc ctgaccaaaa
    36481 tgatgaccag ttacgttatc ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt
    36541 tagtcactat cggcaacgac gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc
    36601 tgatgttcct caaaccgggc atgcaggttc cggtttctca gctgatccgc ggtattaacc
    36661 tgcaatcggg taacgatgct tgtgtcgcca tggccgattt tgccgctggt agccaggacg
    36721 cttttgttgg cttgatgaac agctacgtta acgcactggg cctgaaaaat acccacttcc
    36781 agacggtaca tggtctggat gctgatggtc agtacagctc cgcgcgagat atggcgctga
    36841 tcggccaggc attgatccgt gacgtaccga atgaatactc gatctataaa gaaaaagaat
    36901 ttacgtttaa cggtattcgc cagctgaacc gtaacggcct gttatgggat aacagcctga
    36961 atgtcgacgg catcaaaacc ggacacactg acaaagcagg ttacaacctt gttgcttctg
    37021 cgactgaagg ccagatgcgc ttgatttctg cggtgatggg cggacgtact tttaaaggcc
    37081 gtgaagccga aagtaaaaaa ctgctaacct ggggcttccg tttcttcgaa accgttaacc
    37141 cactgaaagt aggtaaagag ttcgcctctg aaccggtttg gtttggtgat tctgatcgcg
    37201 cttcgttagg ggttgataaa gacgtgtacc tgaccattcc acgtggtcgc atgaaagatc
    37261 tgaaagccag ctatgtgctg aacagcagtg aattgcatgc gccgctgcaa aagaatcagg
    37321 tcgtcggaac tatcaacttc cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg
    37381 tgttgcaaga aatcccggaa ggtaacttct tcggcaaaat cattgattac attaaattaa
    37441 tgttccatca ctggtttggt taaaaattga acacttgaaa gtgtaatttc cgtccccata
    37501 tactaagcat cagtaaaaaa ctcccgcctt ctggcgggag ttgctattta attacgttac
    37561 gccggagctg acatgaaaac caaacttaac gaactgcttg aattccctac tccttttact
    37621 tacaaagtta tggggcaggc gttacctgag ctggttgatc aggtggttga agtggtacag
    37681 cgccatgcgc caggtgacta caccccaacg gtaaaaccaa gcagcaaagg caactaccac
    37741 tcggtatcta tcactatcaa cgccactcat atcgagcagg ttgaaacact gtatgaagaa
    37801 ctaggcaaaa tcgatattgt ccgcatggtt ctgtaactcg cttctccgtt acccggcccc
    37861 tggtcgggta actcccctct cctcgctgtg atatactttt cccccacttt tactcattct
    37921 ccacggagat gcagttttgt atcaggataa aattcttgtc cgccagctcg gtcttcagcc
    37981 ttacgagcca atctcccagg ctatgcatga attcaccgat acccgcgatg atagtaccct
    38041 tgatgaaatc tggctggtcg agcactatcc ggtattcacc caaggtcagg caggaaaagc
    38101 ggagcacatt ttaatgccgg gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt
    38161 gacttatcac gggccggggc aacaggtgat gtatgtgttg cttaacctga aacgccgtaa
    38221 actcggtgtg cgtgaactgg tgaccttgct tgagcaaaca gtggtgaata ccctggctga
    38281 actgggtata gaagcgcatc ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa
    38341 aatttgctca ctgggtttac gtattcgacg cggttgttca ttccacggtc tggcattaaa
    38401 cgtcaatatg gatctttcac catttttacg tattaatcct tgtgggtatg ccggaatgga
    38461 aatggctaaa atatcacaat ggaaacccga agcgacgact aataatattg caccacgttt
    38521 actggaaaat attttagcgc tactaaacaa tcctgacttc gaatatatta ccgcttaatt
    38581 gcatacctca atggcccaat ttacactggg tcattactca taatacctgc taatgcttta
    38641 catctccttc gcgtttgtat actttcttta tcacttgcag aaatgcggtg ctacgccttg
    38701 ctccgtaact caccccattg attaacctat tgaaatagtt gattatggtg agcaaaagcg
    38761 ttccgttcca attagacata agattattgt aattttttta aatattattt ttccatgaat
    38821 aaaattggag taagtgcgtg gatagtaata atcaaattga accctgttta agcagaaagt
    38881 cgtcagaagg taaaccacaa atatttacaa ccttacgaaa tattgatctt aaccttctga
    38941 ctatttttga agctgtatat gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc
    39001 tgaccccctc ggcaatcagt cagtctattc agaaactgcg cgttatattc cctgacccat
    39061 tgtttattcg caaaggccag ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt
    39121 atatcagtca gggccttgag tccattcttg gcgcgctgga tatcgaagga agctatgata
    39181 agcaacgaac gataactatt gcgaccactc cctcggtcgg agccctggtc cttcctgtca
    39241 tctatcgggc gattaaaact cactatccgc agcttttact gcgcaacccg cccatcagcg
    39301 acgcggaaaa ccaactcagt cagtttcaaa ccgatctcat catcgataac atgttttgca
    39361 ccaatcgtac ggtgcaacat catgttctgt tcaccgacaa tatggtgtta atttgccgcg
    39421 agggaaatcc actactctct ttagaagatg acagagagac tatcgacaac gctgcgcatg
    39481 tactcctgtt accggaaggg caaaatttca gcggtctgcg gcagagagtt caagagatgt
    39541 ttccggaccg gcaaatcaat ttcaccagct acaacatttt gacaatcgct gcactggttg
    39601 ccaacagtga catgttagcg attattccca gccgttttta taacctgttt agtcgttgct
    39661 ggccgctgga aaaactgcct tttccgtcct taaatgagga gcaaatagat ttctctatcc
    39721 actacaacaa attcagcctg cgtgatccca tcctgcacgg ggtaatagat gttatccgta
    39781 atgcattttg aaaaagtatt gttacagcca gatcacagcg cagaacgata aaatggcaca
    39841 atccaacaac aattttacat tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg
    39901 atatactgcc agtcgttaat tcaaaaatag ttgataatta caacaatcta ttgaattgaa
    39961 acgctttcct tcgtaattcg caactggaac acgcacgcta tgagtaaacc cattgtgatg
    40021 gaacgcggtg ttaaataccg cgatgccgat aagatggccc ttatcccggt taaaaacgtg
    40081 gcaacagagc gcgaagccct gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg
    40141 gactctacac gtatccaggg catcaaagcc gcaatgcgca aaaatggcct gcattctgtc
    40201 tgcgaggaag cctcctgccc taacctggcg gaatgcttca accacggcac agcaacgttt
    40261 atgatcctcg gcgctatttg tacccgccgt tgtccgttct gtgatgttgc ccacggtcgc
    40321 ccggtagctc ctgatgccaa tgaaccagtg aaactggcgc agaccattgc cgatatggcg
    40381 ctgcgttatg tggttatcac ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccag
    40441 cactttgcgg attgcattac tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact
    40501 ctggtgccgg atttccgtgg tcgtatggat cgtgctctgg atattctgac tgcaacgcca
    40561 ccagatgtgt tcaaccataa cctggaaaac gtaccgcgta tttaccgtca ggtacggcct
    40621 ggtgcagatt acaactggtc gctgaagctg ctggaacgct ttaaagaagc gcatccggaa
    40681 atcccgacca agtctggtct gatggtggga ctgggtgaaa ccaatgaaga aattattgag
    40741 gtaatgcgcg acctgcgccg tcatggtgtg acgatgttaa cgctggggca atatttgcag
    40801 ccaagccgcc atcacctgcc ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg
    40861 aaagccgaag cgctggcgat gggctttacc catgctgcat gcggtccgtt tgtccgctct
    40921 tcttaccacg ccgatttgca ggcgaaaggg atggaagtta agtaatcctg taataattac
    40981 ttacatctgt ctgataaaaa acccgctttt tg
//
LOCUS       DAJTVJ010000020        40830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000020 DAJTVJ010000000
VERSION     DAJTVJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.020"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            74..754
                     /locus_tag="OO448_001066"
                     /pseudo
     CDS             74..754
                     /locus_tag="OO448_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
     gene            756..992
                     /locus_tag="OO448_001067"
     CDS             756..992
                     /locus_tag="OO448_001067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336339.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1165..1840)
                     /locus_tag="OO448_001068"
                     /pseudo
     CDS             complement(1165..1840)
                     /locus_tag="OO448_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1985..2593)
                     /locus_tag="OO448_001069"
     CDS             complement(1985..2593)
                     /locus_tag="OO448_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6336340.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2777..3094)
                     /gene="metJ"
                     /locus_tag="OO448_001070"
     CDS             complement(2777..3094)
                     /gene="metJ"
                     /locus_tag="OO448_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6336341.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3371..4531
                     /gene="metB"
                     /locus_tag="OO448_001071"
     CDS             3371..4531
                     /gene="metB"
                     /locus_tag="OO448_001071"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6336342.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4534..6966
                     /gene="metL"
                     /locus_tag="OO448_001072"
     CDS             4534..6966
                     /gene="metL"
                     /locus_tag="OO448_001072"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6336343.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7315..8205
                     /gene="metF"
                     /locus_tag="OO448_001073"
     CDS             7315..8205
                     /gene="metF"
                     /locus_tag="OO448_001073"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6336344.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8534..10714
                     /gene="katG"
                     /locus_tag="OO448_001074"
     CDS             8534..10714
                     /gene="katG"
                     /locus_tag="OO448_001074"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6336345.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10808..11713
                     /gene="yijE"
                     /locus_tag="OO448_001075"
     CDS             10808..11713
                     /gene="yijE"
                     /locus_tag="OO448_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6336346.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11740..12357)
                     /locus_tag="OO448_001076"
     CDS             complement(11740..12357)
                     /locus_tag="OO448_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6336347.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12631..13734)
                     /gene="gldA"
                     /locus_tag="OO448_001077"
     CDS             complement(12631..13734)
                     /gene="gldA"
                     /locus_tag="OO448_001077"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR6336348.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13745..14407)
                     /gene="fsa"
                     /locus_tag="OO448_001078"
     CDS             complement(13745..14407)
                     /gene="fsa"
                     /locus_tag="OO448_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6336349.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14419..16920)
                     /gene="ptsP"
                     /locus_tag="OO448_001079"
     CDS             complement(14419..16920)
                     /gene="ptsP"
                     /locus_tag="OO448_001079"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6336350.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLWALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17229..18308
                     /locus_tag="OO448_001080"
     CDS             17229..18308
                     /locus_tag="OO448_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6336351.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTLVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18323..18643
                     /locus_tag="OO448_001081"
                     /pseudo
     CDS             18323..18643
                     /locus_tag="OO448_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18694..20990
                     /locus_tag="OO448_001082"
                     /pseudo
     CDS             18694..20990
                     /locus_tag="OO448_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            20956..21834
                     /locus_tag="OO448_001083"
     CDS             20956..21834
                     /locus_tag="OO448_001083"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6336352.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21836..22177
                     /locus_tag="OO448_001084"
     CDS             21836..22177
                     /locus_tag="OO448_001084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6336353.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22164..23015)
                     /locus_tag="OO448_001085"
     CDS             complement(22164..23015)
                     /locus_tag="OO448_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6336354.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23230..24963)
                     /locus_tag="OO448_001086"
     CDS             complement(23230..24963)
                     /locus_tag="OO448_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6336355.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25146..27797)
                     /gene="ppc"
                     /locus_tag="OO448_001087"
     CDS             complement(25146..27797)
                     /gene="ppc"
                     /locus_tag="OO448_001087"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6336356.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28149..29300)
                     /gene="argE"
                     /locus_tag="OO448_001088"
     CDS             complement(28149..29300)
                     /gene="argE"
                     /locus_tag="OO448_001088"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6336357.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29454..30458
                     /gene="argC"
                     /locus_tag="OO448_001089"
     CDS             29454..30458
                     /gene="argC"
                     /locus_tag="OO448_001089"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6336358.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30466..31242
                     /gene="argB"
                     /locus_tag="OO448_001090"
     CDS             30466..31242
                     /gene="argB"
                     /locus_tag="OO448_001090"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6336359.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31303..32676
                     /gene="argH"
                     /locus_tag="OO448_001091"
     CDS             31303..32676
                     /gene="argH"
                     /locus_tag="OO448_001091"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6336360.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32943..33860
                     /gene="oxyR"
                     /locus_tag="OO448_001092"
     CDS             32943..33860
                     /gene="oxyR"
                     /locus_tag="OO448_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6336361.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(33843..35243)
                     /gene="sthA"
                     /locus_tag="OO448_001093"
     CDS             complement(33843..35243)
                     /gene="sthA"
                     /locus_tag="OO448_001093"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6336362.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35564..36214
                     /gene="fabR"
                     /locus_tag="OO448_001094"
     CDS             35564..36214
                     /gene="fabR"
                     /locus_tag="OO448_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6336363.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36214..36573
                     /locus_tag="OO448_001095"
     CDS             36214..36573
                     /locus_tag="OO448_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6336364.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36613..37713)
                     /gene="trmA"
                     /locus_tag="OO448_001096"
     CDS             complement(36613..37713)
                     /gene="trmA"
                     /locus_tag="OO448_001096"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6336365.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37827..38017
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38082..39926
                     /gene="btuB"
                     /locus_tag="OO448_001097"
     CDS             38082..39926
                     /gene="btuB"
                     /locus_tag="OO448_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6336366.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39871..40728
                     /gene="murI"
                     /locus_tag="OO448_001098"
     CDS             39871..40728
                     /gene="murI"
                     /locus_tag="OO448_001098"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6336367.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9431 a        10170 c        11212 g        10017 t
ORIGIN      
        1 atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct
       61 ggaaatacgc cgggaatatg atgaatgggg caacctgctg aatgaagaga acccgcatca
      121 gctgcagcag cttattcgtt tgccggggca gcagtatgat gaggagtccg gcctgtatta
      181 taaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg atccgattgg
      241 gctgaagggg ggatggaacc tgtatacata tccgctgagc ccggtgaata gcatggctcc
      301 attaggatta tatgaattta aatcaaaaaa tattgatgat attggaatat ttgcattggc
      361 aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa tatgtaagaa
      421 gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata gtgtagactt
      481 gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca ctcacggttt
      541 ttactctgac gataaaggaa ataaagtaac aaaagaaaat gatgtttatg atagtctaaa
      601 tttttcaagc aaagatttaa cgaattctta tatgaatgga atgggaaaaa aagaatacag
      661 tagttacttg ggaacaccaa ataacaccta tctaaaatat aatcccaaag ctaaagggaa
      721 tggagttaca attatcaggc aagggagtaa ttaaaatgaa ggcaatacaa tgttgttatt
      781 tccttgcttt agtctccagt catttaatgg ctggagttat gggtgatgat aatatatttt
      841 taagtaattc ggattcagct gctttatatt gtgtggctaa aatatttcca gatggagaaa
      901 atattttaat atataaagta aatataatta atgctagttc aaaaaaagat gtttttttta
      961 tgaaagccca acatcaaccc acaatgacat aatggggggc gggggaatgt catatgccta
     1021 tgaatatgat gtaaaatata atatatgtca tttgagaaaa aagagcagaa tgaggtaaaa
     1081 atctttggct gtgtattcag tctggtaatc atccaaggaa agcttatcag aagggtgatg
     1141 actccaatta gtagaacata tattttcgat aaaagcaccg atgacttttt catgaagttc
     1201 tacagaacgc gagaaacaga tggttctgcg ggccagacgt ttgattcggg ttctcagggt
     1261 cagattgtta cgctcaatgt gctgagtaaa aatcttacca gtcagatgct tctccttcgg
     1321 taactctcta gcgtaactac cccattcgtc agtggtcaac atgccgatgg taaaagggta
     1381 agcagcgccc gcaattcacg acaggtttcg tcagtccggg ggccaaaagt gtaggccaga
     1441 acaccaccag ttttcgtgtt gtacacgtac cagagccagt gctgtcgcgc tttactgccg
     1501 acaaagctcc attgctgact aagtttgcag atgagtgcca catcagcatg agcgaccggg
     1561 gatgacgtta ttcttcgtgg cgagagtttt ttaaagttcg gatgactgta ttaatgccga
     1621 ttttcagcgt acgggcagta tcgcgaaccc cggcaccgtt gaaagtcatt tcagcgattt
     1681 ggtctttaac tcccggttta cggggctcgt aacagtaact gagctgaaat acccggtgac
     1741 agtcacggcc gcggaatcgc tcatgtcttt ttgggttctg cccgtgacgg taaacctgaa
     1801 cggactggca acgggggcaa tgaaggttaa ctgtgaccat gaaacaacct taaaagcgag
     1861 tattatatcc ttaattcaac taattggagg catcaccgtt taccggagcg acagcttgcg
     1921 gaaaggtcgt gcaactacaa agcaacaaac agactacggc gggaattatt tttttcatca
     1981 tccattacca cgaaaacagc ggccactctt tggcgactgt ggtgagttgt ggcgcgtcga
     2041 aaacggcttg tggtgaaact accgtttcct caagcggaat atttttgccg tagcgttctt
     2101 tgagcttagc ctggaccagc gggttgctga gatcgaactc tttcaacttc tgctgctcgc
     2161 ccacgcgcat tcccagcgcg ttctggtaaa ccttccacac cacttccgaa cagtactggc
     2221 gatcgtcgct ccatgagaag ctgaaatcat acggtttacc gagataacgt tttgccgttt
     2281 gcgccagttt ttgctgttgt tcaacactca gtccgccttc aacgcggcga acaacgtatt
     2341 tgcccttttc accatgggcg atccactgct tgagcggggg gtatttcacc gggccgaccg
     2401 cttcaaaaac gtagggcttt ttgttgcgta tcaccagcat accggtgtgg ctataatcgg
     2461 aatgggtcgc cagttggatc gctttacttt gcgatgagcg agagatctga aagatgatgt
     2521 cgccggtttg tggctgccag gcaaaggcag gtacagaaac cagcaggctg aggatcagca
     2581 gcctgttttt catagttaaa cgtccatgta taaaaagcgg tgggtcgcag acaacgtgct
     2641 cgttgtttat gccggatgcg gcgtgaacgc cttatccggc ctacaagttc gtgcaaattc
     2701 aataaattgc aatatgacgt aggcctgata agcgtagcgc atcaggcgat tccactccgc
     2761 gccgctcttt tttgctttag tattcccacg tctccgggtt aatccccatc tcacgcatga
     2821 tctcttttgc cgcttccggg atttcgtcgc tgcgctcttt acgcagatcg gcatcatccg
     2881 gcaaaggttg cccggtaaag gcatgcagaa acgcttcgca cagcagctcg ctgttggtag
     2941 cgtgacgcag gttgttcacc tgacgacgcg tgcgttcatc ggtgaggatt tttaacacct
     3001 taagaggaat ggaaaccgta atctttttga cttgttcact cttcttgccg tgctcagcgt
     3061 atgggctgat atattcgccg ctccattcag ccatgagata cttaatcctc ttcgtcaata
     3121 aattgagacc agaccacaca atttatgtgg gtactgaccg taaacccgca tagtttaccg
     3181 tacaggcgtt accgtgacat cgtgtaatgc acctgtcggc gtgataatgc atataatttt
     3241 aacggctatt tgggatttgc tcaatctata cgcaaagaag tttagatgtc cagatgtatt
     3301 gacgtccatt aacacaatgt ttactctggt gcctgacatt tcaccgacaa agcccaggaa
     3361 acttcatcac atgacgcgta aacaggccac catcgcagtg cgtagcgggt taaatgacga
     3421 cgaacagtat ggttgcgttg tccctcccat ccatctttcc agcacctata actttaccgg
     3481 atttaatgaa ccgcgcgcgc atgattactc gcgtcgcggc aacccaacgc gcgatgtggt
     3541 tcagcgtgcg ctggcagaac tggaaggcgg tgctggcgca gtacttacta ataccggcat
     3601 gtccgcgatt cacctggtaa cgaccgtctt tttgaaacct ggcgatctgc tggttgcgcc
     3661 gcacgactgc tacggcggta gctatcgcct gttcgacagt ctggcgaaac gcggttgcta
     3721 tcgcgtgttg tttgttgatc aaggcgatga acaggcatta cgggcagcgc tggcagaaaa
     3781 acccaaactg gtactggtag aaagcccaag taatccattg ttacgcgtcg tggatattgc
     3841 gaaaatctgc catctggcaa gggaagtcgg ggcggtgagc gtggtggata acaccttctt
     3901 aagcccggca ttacaaaatc cgctggcatt aggtgccgat ctggtgttgc attcatgcac
     3961 gaaatatctg aacggtcact ctgacgtggt ggctggcgtg gtgattgcta aagacccgga
     4021 cgttgtcact gaacttgcct ggtgggcaaa caatattggc gtgacgggcg gcgcgtttga
     4081 cagctatctg ctgctacgcg ggttgcgaac gctggtgccg cgtatggagc tggcgcagcg
     4141 caacgcgcag gcgattgtga aatatctgca aacccagccg ttggtgaaaa aactgtatca
     4201 cccgtcgctg ccggaaaatc aggggcatga aattgccgcg cgtcagcaaa aaggctttgg
     4261 cgcaatgttg agttttgaac tggatggcga tgagcagacg ctgcgtcgtt tcctgagcgg
     4321 gctgtcgttg tttacgctgg cggaatcatt agggggagtg gaaagtttaa tctctcacgc
     4381 cgcaaccatg acacatgcag gcatggcacc agaagcgcgt gctgccgccg ggatctccga
     4441 gacgctgctg cgtatctcca ccggtattga agatggcgaa gatttaattg ccgacctgga
     4501 aaatggcttc cgggctgcaa acaaggggta aaaatgagtg tgattgcgca ggcaggggcg
     4561 aaaggtcgtc agctgcataa atttggtggc agtagtctgg ctgatgtgaa gtgttatttg
     4621 cgtgtcgcgg gcattatggc ggagtactct cagcctgacg atatgatggt ggtttccgcc
     4681 gccggtagca ccactaacca gttgattaac tggttgaaac taagccagac cgatcgtctc
     4741 tctgcgcatc aggttcaaca aacgctgcgt cgctatcagt gcgatctgat tagcggtctg
     4801 ctacccgctg aagaagccga tagcctcatt agcgcttttg tcagcgatct ggaacgcctg
     4861 gcggcgctgc tcgacagcgg tattaacgac gcagtgtatg cggaagtggt gggccacggg
     4921 gaagtatggt cggcacgcct gatgtctgcg gtacttaatc aacaagggct gccagcggcc
     4981 tggcttgatg cccgcgagtt tttacgcgct gaacgcgccg cacaaccgca ggttgatgaa
     5041 gggctttctt acccgttgct gcaacagcta ctggtgcaac atccgggcaa acgtctggtg
     5101 gtgaccgggt ttatcagccg taacaacgcc ggtgaaacgg tgctgctggg gcgtaacggt
     5161 tccgactatt ccgcgacaca aatcggtgcg ctggcgggtg tttctcgtgt aaccatctgg
     5221 agcgacgtcg ccggggtata cagtgccgac ccgcgtaaag tgaaagatgc ctgtctgctg
     5281 ccgttgctgc gtctggatga agccagcgaa ctggctcgtc tggcggctcc cgttcttcac
     5341 gcccgtactt tacagccggt ttctggcagc gaaatcgacc tgcaactgcg ctgtagctac
     5401 acgccggatc aaggttccac gcgcattgaa cgcgtgctgg cctccggtac tggtgcgcgt
     5461 attgtcacca gccacgatga tgtctgtttg attgagtttc aggtgcccgc cagtcaggat
     5521 ttcaaactgg cgcataaaga gatcgaccaa atcctgaaac gcgcgcaggt acgcccgctg
     5581 gcggttggcg tacataacga tcgccagttg ctgcaatttt gctacacctc agaagtggcc
     5641 gacagtgcgc tgaaaatcct cgacgaagcg gggttacctg gcgaactgcg cctgcgtcag
     5701 gggctggcgc tggtggcgat ggtcggtgca ggcgtcaccc gtaacccgct gcattgccac
     5761 cgcttctggc agcaactgaa aggccagccg gtcgaattta cctggcagtc cgatgacggc
     5821 atcagcctgg tggcagtact gcgcaccggc ccgaccgaaa gcctgattca ggggctgcat
     5881 cagtccgtct tccgcgcaga aaaacgcatc ggcctggtat tgttcggtaa gggcaatatc
     5941 ggttcccgtt ggctggaact gttcgcccgt gagcagagca cgctttcggc acgtaccgac
     6001 tttgaatttg tgctggcagg cgtggtggac agccgccgca gtctgttgag ctatgacggg
     6061 ctggacgcca gccgcgcgtt agccttcttc aacgatgaag cggttgagca ggatgaagag
     6121 tcgttgttcc tgtggatgcg cgcccatccg tatgatgatt tagtggtgct ggacgttacc
     6181 gccagccagc agcttgctga tcagtatctt gatttcgcca gccacggttt ccacgtcatc
     6241 agcgccaaca aactggcggg agccagcgac agcaataaat atcgccagat ccacgacgcc
     6301 ttcgaaaaaa ccgggcgtca ctggctgtac aatgccaccg tcggtgcggg cttaccgatc
     6361 aaccacaccg tgcgcgatct gatcgacagc ggcgatacta ttttgtcgat cagcgggatc
     6421 ttctccggca cgctctcctg gttgttcctg caattcgacg gtagcgtgcc gtttaccgag
     6481 ctggtggatc aggcgtggca gcagggctta accgaacctg acccgcgtga cgatctctct
     6541 ggcaaagacg tgatgcgcaa gctggtgatt ctggcgcgtg aagcaggtta caacatcgaa
     6601 ccggatcagg tacgcgtgga gtcgctggtg cctgcccatt gcgaaggcgg cagcatcgac
     6661 catttctttg aaaatggcga tgaacttaac gagcagatgg tgcaacggct ggaagcggcc
     6721 cgcgaaatgg ggctggtgct gcgctacgtg gcgcgtttcg atgccaatgg caaagcgcgt
     6781 gtgggcgtgg aagcggtgcg tgaagaccat ccgctggcat cactgctgcc gtgcgataac
     6841 gtctttgcca tcgaaagccg ctggtatcgc gataatccgc tggtgatccg tgggcctggc
     6901 gctgggcgcg acgtcaccgc cggggcgatt cagtcggata tcaaccggct ggcacagttg
     6961 ttgtaatttc agaaatttaa taatgcccgg tactcatgtt ttcgggttta tggtttctaa
     7021 tgaaatatat tgaattatca taggattagg ccggattaag cgtttacgac gaatccggca
     7081 agaagcaata agtacatggt tagtttatat ttgcagtccg gtttgctttg cataccggat
     7141 tttctttttc ttaccatcct gaagtttttt catcttccct gatttttcct caccatcatt
     7201 ggtcattttt cggttgacgc ccttcggctt ttccttcatc tttacatctg gacgtctaaa
     7261 cggatagatg tgcacaacac aacatataac tacaagcgat tgatgaggta aggtatgagc
     7321 ttttttcacg ccagccagcg ggatgccctg aatcagagcc tggcagaagt ccaggggcag
     7381 attaacgttt cgttcgagtt tttcccgccg cgtaccagtg aaatggagca gaccctgtgg
     7441 aactccatcg atcgccttag cagcctgaaa ccgaagtttg tatcggtgac ctatggcgcg
     7501 aactccggcg agcgcgaccg tacgcacagc attattaaag gcattaaaga tcgcactggt
     7561 ctggaagcgg caccgcatct tacttgcatt gatgcgacgc ccgacgagtt gcgcaccatt
     7621 gcacgcgact actggaataa cggtattcgt catatcgtgg cgctgcgtgg cgatctgccg
     7681 ccgggaagtg gtaagccaga aatgtatgct tctgacctgg tgacgctatt aaaagaagtg
     7741 gcagatttcg atatctccgt ggcggcgtat ccggaagttc acccggaagc aaaaagcgct
     7801 caggcggatt tgcttaatct gaaacgcaaa gtcgatgccg gagccaaccg cgcgattact
     7861 cagttcttct tcgatgtcga aagctacctg cgttttcgtg accgctgtgt atcggcgggc
     7921 attgatgtgg aaattattcc gggaattttg ccggtatcta actttaaaca ggcgaagaaa
     7981 tttgccgata tgaccaacgt gcgtattccg gcgtggatgg cgcaaatgtt cgacggcctg
     8041 gatgatgatg ccgaaacccg caaactggtg ggcgcgaata ttgccatgga tatggtgaag
     8101 attttaagcc gtgaaggagt gaaagatttc cacttctata cgcttaaccg cgctgaaatg
     8161 agttacgcga tttgccatac gctgggtgtt cgacctggtt tataaatagt gtggcttttg
     8221 tgaaaatcac acagtgatca caaattttaa acagagcaca aaatgctgcc tcgaaatgag
     8281 ggcgggaaaa taaggttgtc agccttgttt tctccctcat tacttgaagg atatgaagct
     8341 aaaacccttt tttataaagc atttgtccga attcggacat aatcaaaaaa gcttaattac
     8401 gatcaatttg acctacatct ctttaaccaa caatatgtaa gatctcaact atcgcatccg
     8461 tggattaatt caattataac ttctctctaa cgctgtgtat cgtaacggta acactgtaga
     8521 ggggagcaca ttgatgagca cgtcagacga tatccataac acgacagcca ctggcaaatg
     8581 cccgttccat cagggcggtc acgaccagag cgcgggggcg ggcacaacca ctcgcgactg
     8641 gtggccaaat caactccgtg ttgacctgtt aaaccaacat tctaatcgtt ctaacccgct
     8701 gggtgaggac tttgactacc gcaaagaatt cagcaaatta gattactacg gcctgaaaaa
     8761 agatctgaaa gccctgctga cagaatctca gccgtggtgg ccagccgact ggggcagcta
     8821 cgccggtctg tttattcgta tggcctggca cggcgcaggt acttaccgtt caatcgatgg
     8881 acgcggcggc gcgggtcgtg gtcagcaacg ttttgcaccg ctgaactcct ggccggataa
     8941 cgtaagcctc gataaagcgc gtcgcctgtt gtggccaatc aaacagaaat atggtcagaa
     9001 aatctcctgg gccgacctgt ttatcctcgc aggtaacgtg gcgctggaaa actccggctt
     9061 ccgtaccttc ggttttggtg ccggtcgtga agacgtctgg gaaccggatc tggatgttaa
     9121 ctggggtgat gaaaaagcct ggctgactca ccgtcatccg gaagcgctgg cgaaagcacc
     9181 gctgggtgca accgagatgg gcctgattta cgttaacccg gaaggcccgg atcacagcgg
     9241 cgaaccgctt tctgcggcag cagctatccg cgcgaccttc ggcaacatgg gcatgaacga
     9301 cgaagaaacc gtggcgctga ttgcgggtgg tcatacgctg ggtaaaaccc acggtgccgg
     9361 tccgacatca aacgtaggtc ctgatccaga agctgcaccg attgaagaac aaggtttagg
     9421 ctgggcgagc acttacggca gcggcgttgg cgcagatgcc attacctctg gtctggaagt
     9481 tgtctggacc cagacgccga cccagtggag caactatttc ttcgaaaacc tgttcaaata
     9541 tgagtgggta cagacccgca gtccggctgg cgcaatccag ttcgaagcgg tagacgcacc
     9601 ggaaattatc ccggatccat tcgatccgtc gaagaaacgt aaaccgacga tgctggtgac
     9661 cgacctgacg ctgcgttttg atcctgagtt cgagaagatc tctcgtcgtt tcctcaacga
     9721 tccgcaggcg ttcaacgaag cctttgcccg tgcctggttc aaactgacgc acagggatat
     9781 ggggccgaaa tctcgctaca tcgggccgga agtgccgaaa gaagatctga tctggcaaga
     9841 tccgctgccg cagccgatct acaacccgac cgagcaggac attatcgatc tgaaattcgc
     9901 gattgcggat tctggtctgt ctgttagtga gcgggtatcg gtggcctggg catctgcttc
     9961 taccttccgt ggtggcgaca aacgcggtgg tgccaacggt gcgcgtctgg cattaatgcc
    10021 gcagcgcgac tgggatgtga acgccgcagc cgttcgtgct ctgcctgttc tggagaaaat
    10081 ccagaaagag tctggtaaag cctcgctggc ggatatcatc gtgctggctg gtgtggttgg
    10141 tgttgagaaa gccgcaagcg ccgcaggttt gagcattcat gtaccgtttg cgccgggccg
    10201 cgttgatgcg cgtcaggatc agactgacat tgagatgttc gagctgctgg agccaattgc
    10261 tgacggtttc cgtaactatc gcgctcgtct ggatgtttcc accaccgagt cactgctgat
    10321 cgacaaagcc cagcaactga ccctgaccgc gccggaaatg accgcgctgg tgggcgggat
    10381 gcgcgtactg ggtgccaact tcgatggcag caaaaacggc gtcttcactg accgcgttgg
    10441 cgtattgagc aatgacttct tcgtgaactt gctggatatg cgttacgagt ggaaagcgac
    10501 cgacgaatcg aaagagctgt ttgaaggccg tgaccgcgaa accggcgaag tgaaatacac
    10561 tgccagccgt gcggatctgg tatttggttc taactctgtc ctgcgtgcgg tggctgaagt
    10621 ttacgccagc agcgatgctc acgagaagtt tgttaaagac ttcgtggcgg catgggtgaa
    10681 agtgatgaac ctcgaccgtt tcgacctgct gtaatctgac cttcttcagc gactgccttt
    10741 caggcagtcg ctgaagtttc tttaccggcg tatagtgtcc acaggaaaac tacacactgg
    10801 atctctcatg tctgccgcag gaaagagcaa cccactggca atcagtggcc tggttgtgct
    10861 cacacttatc tggagttata gctggatttt catgaagcaa gtcaccagct acatcggtgc
    10921 cttcgacttt accgccttac gctgcatttt cggcgctctc gttttattca tcgtcctttt
    10981 attacgtggt cgcggaatgc gcccgacacc gtttaaatac accttagcca ttgccctgtt
    11041 acaaacctgc gggatggttg gcctggcgca gtgggcgctg gtcagcggcg gagcggggaa
    11101 ggtggcgatc ctaagctata ccatgccgtt ctgggtggtg attttcgccg cgttgtttct
    11161 cggtgaacgc ctgcgacgtg ggcaatattt cgcgatactg attgccgctt tcggcttatt
    11221 tttggtgttg cagccgtggc aactcgattt ctcttcgatg aaaagtgcca tgctggcaat
    11281 cctctccggc gtcagttggg gggcgagcgc gattgttgct aaacgtctgt atgcccgtca
    11341 tccgcgcgtg gatttattgt cgttaacatc ctggcagatg ctctatgcgg cgctggtgat
    11401 gagtgtggtc gctttactgg tgccgcaacg tgaaattgac tggcagccca ccgtgttctg
    11461 ggcgctggcc tacagtgcga ttctggcgac ggcactggcg tggagcttat ggttgtttgt
    11521 attgaaaaac ttgcctgcca gtattgccag cttaagcaca ctggccgttc ccgtttgcgg
    11581 cgtactcttt tcctggtggc tgctcggcga gaatccgggg gccgttgaag gtagcggtat
    11641 tgtgctgatt gtgatggcac tggcgctggt gagccgtaag aaaaaagaag ccgttagtgt
    11701 aaaaaggatc tgaatttttt cttcatgtgg ggcgatcgct tatttaacaa aataacgata
    11761 atgccccacc atgcgccagc taaacagcac atcttcttcc tgcgcacctg cgccaatgtt
    11821 atgtatcacc agcggcgtac cgtcgcgggc gaagccatct gaaaccaccc caatatgtgc
    11881 cagcccgtta tctaatcgcc aggagacaat atcgcccgct tgatagtcac tggggttctt
    11941 gctgatgggg cgtgttttat cgtggtgggt aaaccaggtt tccagattag gcacccgacg
    12001 gtgatcgatg ttgctgtccg ggcgctttaa ctgccatttt tgcgggtact cggcaaaatt
    12061 cttcgccata tcttcgtgca ccagtttctg caaatcgacc ttctggctgc gcaatgcacg
    12121 gatcaccaca tcggaacata ccccgcgttt ttgcgggaca tcaccgccag gataggtaag
    12181 ctgcatatac gccggatcgt aaaatagcgt gctgccgatt tgctgtctgg caccgtctgc
    12241 gatggcaagg ttggtattgg cctggatttg taccacggtt ggtggaacgg cgggagattt
    12301 taaggagtgg ctggtaaatg ccgttagcag gctgagcagc gccagtgaag ctttcatctg
    12361 gttcgtccgt gaaaataaga gtcacagatt cgaccttccg ggataacgat tgttctgtag
    12421 agaaaatgtc cagaagcgga taaaaaactc ctgaacaacg tgggagtttc agattactgg
    12481 cacagagtgc cggatgcggc gtgaacgcct tatctgtcct aaaaaaaaag aaaattcaat
    12541 atattgcaga gattgcgtag gcctgataag cgtagcgcat caggcaattt tgcgttcaaa
    12601 ctcccggaca agccgggagt ttggagtagg ttattcccac tcttgcagga aacgctgacc
    12661 gtactggtcg gctaccagca gagcggcgta aacctgatct ggcgtcgcgc cgccaggcat
    12721 gttgtggatg gtttcacatt ctgcacatgc cgcttcagcc acaattcgca ttttcgccgg
    12781 gacatcttct ttaatatcca gttgagcgag agttattggc aaacctaccg catggctaag
    12841 tgcagctacg gtttcgattt cctcaaccgg tgcgttttcc agaaccagct gcgtcagcgt
    12901 accaaatgcc actttttcac cgtgataata gtgatgcgcg tccgggatag cggtcaggcc
    12961 gttatgcact gcgtgcgccg cagccagacc accactttca aaaccaacac cgctcaaata
    13021 ggtgttcgct tcaatcacgc gctccagcgc cggagtcact acatgctgtt cggcagcaag
    13081 catcgctttt tcgccttctt ccagcagggt gttgtagcac agttcagcca gtgccagcgc
    13141 agcctgggtg cacttgccgc ccgccatggt ggtcgcgccg ctacgagagc aggcacgcgc
    13201 ttcaaaccag gttgccagcg catcgccgat acccgccgct aacagacgtg caggtgcgcc
    13261 agcgacgatt ttggtgtcga caatgaccat attcgggtta tttggcaaca gcagatagcg
    13321 gtcaaactca ccctcatcgg tgtagataac agacaatgcg ctgcacggtg catcggtaga
    13381 ggcgatagtc ggtgcgatcg ctaccggaac acccatgaaa tgtgccagtg ctttggctgt
    13441 atcgagggtt tttccgccac cgataccgag aattgcgcca cactgcgcag tctccgcgat
    13501 gccacgcaga cggtcgatct cattttgcga acatccaccg ccaaacggcg caatttctac
    13561 taccagtcca gcatctttaa agcttttctc gacagtggat tgagcaaaac ctaaaacaaa
    13621 tttgtcaccc accactaacc agcgttctgc cagcggcttc aggtattcgc ccagacgatt
    13681 aatcacatca gcgccctgga tgtatttacc cggtgattga ataatgcggt ccataattgc
    13741 tcctttagag atgagtagtg ccaaatgcgg cattccagtc gtgttcgaac ttctctatag
    13801 ctgactctac cgcaggggtg ttgagcattt gttgcgctac atctaagggc agggtgatgg
    13861 attcacatcc tgccagtaaa cagtccagcg cctgacgcgg cgttttaaag ctggctgcca
    13921 gcaccatgct ttctggcgcg tgcatttcta acagcgcttg cagctcctga accgtacgaa
    13981 tgccgtctcc gccctgggca tctacgcggt taacatacgg agcaacgtat tttgcccctg
    14041 ccagcgcggc cagtaaccct tgtgcagcac tgtacactgc ggtgccaagc gtagtaatac
    14101 cctctttttt cagtatttta attgctgcca gaccttcgga agtcaccggg attttcacca
    14161 caatacccgg aatagcgtcg cgcaggcgct tcgcttcttc caccatcccc tgcgcgtcgc
    14221 ggctcatggt ctgagcaaac agaatgccct catcaccaat cgctttttgc agacgcggca
    14281 gcacttccca tatggactcc ttgctggcag cgataatgct cgggttagtt gtcaccccgg
    14341 caatggggaa tatgcgtgcc agacgttcga cttctgcgac gttagcggtg tccagataca
    14401 gttccatgat gtttcctttt acagttccag ttcatgttgc agcaggctgg cgatagcgtc
    14461 ggcagaggcg gcgttaacca gcgcgttacg gaattcttcg tgcatgatgc ggcgagcgag
    14521 gcgcgagaaa atgcgcatgt gctgatcgcc cgcagcgtgt ttgttcaggg ttaacatgat
    14581 gatgaattgc gcttcatcat caccccagcg caccggcgct tgcagacgcg caacgctgat
    14641 ggtggattgc tcaatgtgtt ctgatttgct gtgtggaatg gcaaaactaa agcccagacc
    14701 ggtagagaaa acggcctcgc gcgcccacaa gtcggcttcc agtttacgcg gatagcggca
    14761 gcgccctgcc agcagcaggt tatcggtcat gcctttgagc acttcttctt tgctgcgcca
    14821 gtcgctttcc agtgtgatgc actcggcggt gaccagcggt gcgtcttgtt gggtcatgcg
    14881 gaattgcgcc agcaggtgct ccacttccag cgaagtacgg caggccattg cctggttgag
    14941 caacttgcgg cactcacggc tatcaagttg cgccatccgc gctttcgccg ccggaattga
    15001 tggtgcgctc atgctgagtt catccagccc taagccgacc agcaacggca gcacggaacc
    15061 tttcgctccc agctcaccgc acagaccaat ccatttaccc tggcgatgca ccgcctgcac
    15121 ggcgtaatcg agcgcccaca agaatgccgg attcaggctg ttgtagtgac gagtaacctt
    15181 agcgttatcg cgatccactg ccagcagata ttgcgtcagg tcattactac caatactaaa
    15241 gaaatcaatc tcttcgcagc attgatcgat gatgaacatc accgacggca cttccagcat
    15301 aatgccgagc tggatcttct catcaaacgg aatgtgttcg ttacgtagtt gctgtttggc
    15361 ttctgccagt ttttctttca cccataagat ctcttccatt gaggagatca tcgggatcat
    15421 gattttcagg ctgccgtgag cggaggcgcg caggatcgag cgtagttgtg tggtaaataa
    15481 cgacgcgtat tcttcataaa tacgcacggc gcgataaccg aggaacgggt ttgcctctgc
    15541 gggaatgttt agataatcaa cgggtttgtc gccgccaatg tccatagtgc gcacaataat
    15601 gctgcgtccg ttggcagact ccagcgcctg acaaaaaatg ttgtacagct cgctttcgcc
    15661 cggtgcactg gtgcgatcca tatagagcat ttcagtgcgg aacaaaccaa cgccttccgc
    15721 accattgccg aatgcggcct gcgcttccac ggagtgagcg atgttagcgg caatttcaat
    15781 gcggataccg tcagcggtac gggcttgttg ggtcagccag acacgctgtt gctcacgcag
    15841 ggcgtcctgt acgcgggctt cttgctgata ataacgagct acggcttctc ctggctcaac
    15901 cacaatcgcc ccggcgttgc cgtcgatata aatcgtttga tgctgccacg gagtgagggc
    15961 atcaatatcc acaccaacca gcgtcggaat gttgaacgaa cgggcaagga tcaccgtatg
    16021 tgaggtggtg ccgccacttt tcagcaacag acctttgagg tgatttttat ccagttcgag
    16081 gaactggctg ggggtcagtt catcagccat acaaatggcg gactgcgtca gtttgcccgg
    16141 tgccgggaag cgttgctcac cgtagatttg ctggagtaac tggaagcaga cgtcgcgtac
    16201 gtccagggca cgttcttgca ggtagctgct gctggaacgg gcgaactctt cgcaaaagtg
    16261 attcgcactg gcgacaatcg cttcggcgca gcttaatccc tcgctgacgc ctgccagtaa
    16321 gtgctgacgt aaagatgtat cgccagccag ggatcggtga gcttccagta tcgcgctggt
    16381 agcaccgtcg ctatccagca gacgaaactc aatgtttttc agtaccagcg tcaggccgtt
    16441 ttccagtgtg gattgctcgg cgtcaacgtc tttggctgcg ggaagattac tcagcgcatt
    16501 gagatctaaa gaagagatcg gcgtcagaat gccgcctgca ctaccgctgc acacggtgcg
    16561 ggcgcggata atttgcggat tcagattggt cagtgaaacc ggcagtggtt ccagttcgtc
    16621 agatttaact tccgccagcg gcgcatcgca gtgggggaat tcatcgcgca gccattggct
    16681 taaacgctgg tgcgcttcct gttcgtcggc cccggaaatc agtaactggc agttatcgcc
    16741 cgccagcgta tcggtgccaa tcagcgcaag ggcgcttttg gcgttgccct tgcggtcagt
    16801 gtgcaggtta tgccactcaa tttgtgatga aaaagtatta cacagcgttt caacgtggct
    16861 tgccggacgc gcatgtacgc cgttaggtag ctcacaaata aattccacaa tcagggccat
    16921 tgcctctctc ccggtacggt tttctgttta caggataaga gcgcacggca acggcctgcc
    16981 atgtgacaaa tctgccaaaa gctggacaaa tgtaatgtaa ccgtcaattt gcgacgcgtc
    17041 tcacaagacg ccgttttgcg gcatgcttcc ggtttatcgc aagttatgag gcggatcgca
    17101 tttttgtact gatattacaa aagtccagta aatggccttt ttatccactg tttgctccgc
    17161 ctgcgattgc ctattgtttc ctccatcaaa aatatggaca tgggctttcg caagcccggg
    17221 agcagcttat gaatgagttg gtgcagatcc tgaaaaatac ccgtcagcat ttaatgacgg
    17281 gcgtttcaca catgattccc ttcgtggtat cgggcggtat tttgctggcg gtttccgtca
    17341 tgttgtatgg caaaggcgca gtgccggatg ccgtagccga tccaaatctg aaaaaactgt
    17401 ttgatatcgg cgttgcgggc ttgacgctga tggtgccttt cctcgccgct tacattggtt
    17461 attccattgc agaacgttct gcgctggctc catgcgctat cggtaccttg gttggtaaca
    17521 gctttggtgc gggcttcttt ggtgcactga tcgccgggat tatcggcggc atcgtggtgc
    17581 attacctgaa gaaaattccg gtgcataaag ttctgcgctc ggtgatgcct atcttcatta
    17641 ttcctatcgt cggcacactg attaccgcag gcatcatgat gtggggcttg ggcgagcctg
    17701 taggggcgtt gaccaacagc ctgactcagt ggcttcaggg gatgcagcag ggcagcattg
    17761 ttatgctggc ggtgatcatg ggtctgatgc tggcgttcga tatgggcggt ccggttaaca
    17821 aagtggccta tgccttcatg ctgatttgcg ttgctcaggg tgtttatacc gtggtggcca
    17881 tcgctgccgt gggtatttgt atcccgccgc tggggatggg gctggcgacg ctgattggtc
    17941 gtaaaaattt ctccgcagaa gagcgcgaaa ccggcaaagc ggcactggtg atgggctgtg
    18001 ttggcgttac tgaaggggcg attcctttcg ccgctgccga tccgctgcgt gtcattcctt
    18061 ccatcatggt cggttcagtt tgtggtgcag taactgcggc gctggtcggt gcgcagtgct
    18121 atgcaggctg gggtggtctg attgtgctgc cggtggttga aggcaagctg ggttatatcg
    18181 cagcagtggc tgtcggagca gtggtgacgg ctgtttgtgt gaacgtgctg aaaagtctgg
    18241 cgcgtaaaaa tgggtcttcg actgatgaaa aagaagacga cctggatttg gattttgaaa
    18301 ttaattaatt gaggaacgag ccatgacgaa aattattgca gtaaccgcat gtccttcagg
    18361 tgttgcccat acctatatgg cagcagaggc gctggaaagt gccgcgaaag cgaaaggctg
    18421 ggaagtgaaa gtagaaacct aggggtcaat tggtctggaa aatgaactga ctgcggaaga
    18481 tgtggcgagc gctgatatgg tgattctgac caaagatatc ggcatcaagt ttgaagagcg
    18541 ttttgctggc aagaccatcg tgcgcgtcaa catcagcgat gcagtaaaac gtgctgacgc
    18601 cattatgagc aaaattgaag ctcacctggc gcaaaccgct taagttcttc tcccgctcgc
    18661 aagggcgggt tcgctttccc acaggagttc ctcatgacga atcgtatctc tcgcctcaaa
    18721 actgcactgt ttgccaatac ccgtgaaatc tcgctggagc gggcgctgct ttataccgcc
    18781 agccatcggc aaaccgaagg cgaaccggtg atattgcgcc gggcgaaagc aacagcgtat
    18841 atccttgaac atgttgaaat ttcgattcgt gatgaagaac tgattgccgg taaccgcacc
    18901 gtaaaaccgc gcgccgggat tatgtcgccg gaaatggacc cttactggct gctgaaagag
    18961 ctggatcaat tcccgacgcg tccgcaggac cgctttgcta tcagcgaaga agataaacgt
    19021 atctaccgtg aagagttgtt cccgtactgg gaaaaacgtt cgatgaaaga tttcatcaac
    19081 gggcagatga cagatgaagt aaaagccgcg accaacacgc agattttcag catcaaccag
    19141 acggataaag gccaggggca cattattatt gattacccac gcctgttgaa tcacggtctg
    19201 ggtgagctgg tggcacagat gcagcaacat tgtcagcaac agccggagaa tcacttttat
    19261 caggccgcgt tgttactgct ggaagcctcg cagaaacaca ttttgcgtta cgccgaactg
    19321 gcggaaacga tggcggcaaa ctgcacagat gcacagcgtc gcgaagaact gctgactatt
    19381 gcggagatct ctcgccataa cgccgaacat aagccgcaga cgttctggca ggcgtgccag
    19441 ttattctggt acatgaacat cattctgcaa tacgaatcca acgccagttc gctgtcttta
    19501 gggcgcttcg accagtatat gttgccgttc tatcaggcat ctttaaccca gggcgaagat
    19561 ccggcgttcc tgaaagaact gctcgaatct ttatgggtga aatgcaacga catcgtgctg
    19621 ttgcgctcca ccagcagcgc gcgttatttc gcaggtttcc cgaccggcta taccgcactg
    19681 ctcggcgggt taaccgagaa cggacgtagc gcggtgaacg tgctttcgtt cctttgcctt
    19741 gacgcctatc aaagcgtgca attaccgcaa ccgaacctcg gcgtgcgcac taacgccttg
    19801 atcgacacgc cgttcctgat gaaaaccgcc gaaaccattc gcctcggcac cggtattccg
    19861 caaatcttta acgatgaagt ggtggtacca gcgttcctca accgtggcgt ttcgctggaa
    19921 gatgcgcgcg actattccgt agtgggctgt gtggaattat ctattcccgg cagaacctac
    19981 ggcttgcatg acatcgcgat gtttaatctg ctgaaagtga tggaaatctg cctgcatgaa
    20041 aatgaaggca atgctgcgct gacttatgaa ggtttactgg agcagatccg cgccaagatc
    20101 agccactaca tcaccctgat ggttgagggc agcaatattt gtgatatcgg ccatcgcgac
    20161 tgggcacctg taccgctgct ctcgtctttt atcagcgatt gtctggaaaa aggtcgcgat
    20221 attaccgatg gcggcgcgcg ttataacttc tccggcgtac aggggatcgg tatcgccaac
    20281 ctgagcgatt ctctccatgc gttgaaaggg atggtttttg agcaacagcg tttaagtttt
    20341 gacgaattgc tgtcggtatt aaaagccaac tttgcaacgc cagaaggcga aaaagtccgt
    20401 gctcgcttaa ttaaccgctt cgagaaatac ggtaacgata tcgacgaggt ggataacatc
    20461 agcgccgaac tgttacgcca ctactgcaaa gaagtggaaa aataccagaa cccgcgcgcg
    20521 gttacttcac gccgggatcg tataccgttt ctgctcacgt cccgttggga tcggtggttg
    20581 gcgcgacgcc agacggtcgt tttgccgggg aacaactggc agacggcggc ttgtcaccta
    20641 tgctgggcca ggacgcacaa gggccaacag cggtactgaa gtcagtcagt aagctcgata
    20701 atacgctgct gtctaacggt acgttgctga acgtgaaatt cactccggcg accctggaag
    20761 gtgaagcggg attacgcaaa ctggccgact tcttacgggc gtttacccag cttaagttgc
    20821 agcatattca gtttaacgtg gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac
    20881 aagattatgc cgggctggtg gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt
    20941 cgaaggagat ccaggatgac atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa
    21001 acgcgccgcg atgatgtggc gcgcattttc aacattcagc gttattcact gaatgacggc
    21061 gagggcattc gtacggtggt cttttttaaa ggctgtccgc atctttgccc gtggtgtgct
    21121 aatccggagt cgatctccgg caaaatccag acggtacgca gagaggcgaa atgtctgcac
    21181 tgtgcgaaat gtttgcgtga tgcggatgaa tgcccctccg gggcgtttga acggattggt
    21241 cgcgatatca gccttgacgc tctggaacgg gaagtgatga aagatgacat tttctttcgc
    21301 acgtccggcg gcggcgtcac gctttctggc ggcgaagtgt taatgcaggc ggagtttgct
    21361 acccgttttt tacagcgact gcggctgtgg ggtgtgtcat gcgccattga aactgccgga
    21421 gacgcgccag caagcaagct gttaccgctg gcgaaattgt gcgatgaagt gctgttcgat
    21481 ttaaaaatta tggacgcgac tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg
    21541 ctggagaatc tgcgtttgct ggtgagtgag ggtgtcaacg tgatcccgcg tttaccgttg
    21601 atccctggtt tcacgctcag cagggagaat atgcagcagg cgctggatgt actgatcccg
    21661 ctgaaaatca ggcagatcca tctgttaccg tttcatcagt acggcgaacc gaaataccgc
    21721 ctgctgggga aaacatggtc gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca
    21781 acgatgcgcg aaatggcaga acgggccgga tttcaggtta ccgtgggagg ttaaaatggc
    21841 atacctggtg gcagtaaccg cctgcgtaag cggcgtggcg catacttata tggcggcgga
    21901 acggctggaa aagttgtgcc agttagagaa gtggggcgtc agcattgaaa ctcagggcgc
    21961 gctgggaacg gagaatcgtt tagcggacga ggatatccgt cgggcggatg ttgctctgtt
    22021 gattacggat atcgagcttg ccggtgccga gcgatttgaa cattgccgct atgtgcaatg
    22081 cagcatctac gcattcctgc gtgagccgca gcgggtaatg agcgcggtgc gcaaagtgct
    22141 ttctgcgccg cagcaaaccc atcttattct ggagtagtcg gtttttctgt cagttggctg
    22201 tggtactgcc ggcgatattc cgacggcgag cgttcggtgt ttttacgaaa cagacggcag
    22261 aagtagttgc tgtcgacaaa gccgcaggcg tgcgccactt cttttacctt caggtcatag
    22321 cctttcaata acgtcttagc gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca
    22381 atggcccccg ttttttgaaa caggtgcgag agatagtttg gtgaaatata gaatgcctgt
    22441 gcgacagatt cgcgggtaag cgcggaggca tagcgttcgt cgatataatc gcgaatagct
    22501 tcaaatagtg cctggctgcg tgaggcggtc tggatttggc tgccgagcaa atcgcggcag
    22561 tggctaagca ggctggctac gataagcctt gccgtttgct gctcctgcgg ctgcatttgc
    22621 atttcattga gcgtttgtag cagaaaagaa cctatacgtg ggccgcgtcg ggcgacgtgt
    22681 tgcttcgcca gattttgata ttgtttgcca tcccattgca cgacgctgaa gccgagttgc
    22741 tgtttgccaa acagcacgct aaaggtggta gcgggtgttt tccattgtgg gaaattccag
    22801 ccaccagcgg caacgtacag cacatcgccg ggaactaacg ttgctccagc gccggtatca
    22861 acaaactcac cttccagcac aatttccagc cgtggaaaat cgacctgata cgcaagatcg
    22921 ggcaccgggc cgttactgct ggcaaagtaa atctggcgca gggacagcgg gccgttgatc
    22981 aggcgggaga gcagatagct gacgccgtga tacatgttat tcccttaggt atgtagacgg
    23041 gaaaacagta actggcatta ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt
    23101 cgatgtaaac attgcggcag ttgtattgcc ggatgtggcg taaacgcctt atccagccta
    23161 ccgccgcgat ctgtaggccg gataagacgc gtcaagcgtc gcatccggca aatagcgcct
    23221 ggctgataat tactgattac ctacctggtc gccatacggc agagtgtcgt aatcgatcag
    23281 cgcgtttttc ttgtacgggt taccaatcca gcgggtagtt tctttgaact gcggattcac
    23341 cactgaacgg gttggatcgt aaccgtcgta agataagccc gccaaatctg accaggtgtg
    23401 gatcagttcc gccaggctgt atttacgatc gacatcctgt gagaaatcac ggggatgagt
    23461 cgcttgccat ttttccgacg tccacagcag gaacggaatg gtgtacatgt ggcgcgtcgg
    23521 gttgtcttcg ttacgcccct gagttttgtg cggcggcgtg tcgtaaacct cttcaccgtg
    23581 gtcagagaag taaaccagga atccgttcgg gtctgccgct ttaaagtctt taatcaggct
    23641 ggcaaccaca tgatcgttat acaggttagc gttgtcataa tcgttatatg attccagctc
    23701 ttctgcgctt aatcctggcg gaacatgatc ggtattgcca tcaaacttgc cctgattttc
    23761 cgggtagcgg tatttgtatt tgatatgcgt acccagcaga tgaacgatga tcagtttctt
    23821 cggcgcaggg tcattcagca catcctggaa cggcttcagc acgttggtgt cgtattcacg
    23881 cgcactctgc gtacgttgct ggttcatgta gtactgcttg tcggtctggc gcgaaaatac
    23941 cgtcagcatg gtattgcggg cggtcatcgt ctgctggttg gtgatccaga aggttttata
    24001 acccgcctgt ttcatcatgt tcatcagcga cggctgcgtc agatacagat ccgggttctt
    24061 ttcattggca aaggtcagcg cctgttgcag gatttcaatg gtgtacggac gagacgtaac
    24121 tacgttatta aacacggtca gattcggatc ggttttatgc agcgcatcca gctccggcgt
    24181 ggtttcacgc ggataaccgt acagactcat gcgtccgcgc tgggtcgact cgccaatcac
    24241 cagcactaaa gtgcgcggtt cgttacccga ttcatctttg aaattagcca gtggcggcaa
    24301 ggcattattt tcattcagta actttgttag cgagtttagt tgctgacgat actgataata
    24361 gccggtcagg aattgccacg gtgcggcagg ctccatacgc gaggccaggt tatccaacgt
    24421 tttctcaaac ggcttgtttt tgataaacgt attcatggcg atcggatgca gaatcaagcc
    24481 ataaagcagg gcaaaagaga caacataacg ccacggcttt ggaatataga ccgggcgcag
    24541 gcgtgtccac agcagaactg ccaccgccgt ataggccagc gcgataagca caattttcag
    24601 gctgaaatac tggcttaaat actcgctggc ttcgttggtg ttggtttcga acatcacaaa
    24661 cagaacgctc tgcgagaact cctgcccgta gatgacgtag tagcatagcg ccgccagaga
    24721 ggccgcccat agcaccacgc cgattactgc ggcaataatt ttaatccgct tcggaaagag
    24781 gaataccggg atcaaccaca gcgagctgaa taacagcgag tcgcgaatgc cgttggtgcc
    24841 actataacca ctgatgtaaa taatggcctg tagcagagta gagaaaaacc aaaagtagag
    24901 cagtgcccaa cccagggctt tccagctaaa aaggggttta gcctggactt ctgtggaatg
    24961 catagtaaga acctgtcttg aaaaaaatat cgccgaatgt aacgacaatt ccttaaggat
    25021 atctgaaggt atattcagaa tttgaataaa atgcagacag aaatatattg aaaacgaggg
    25081 tgttagaaca gaagtatttc agaaaaccct cgcgcaaaag cacgagggtt tgcagaagag
    25141 gaagattagc cggtattacg catacctgcc gcaatcccgg caatagtgac cattaacgct
    25201 tgttcgacgc gaggatccgg ttcctggcct tctttttctg cctggcggga gcggtgcagc
    25261 aactcggcct gcaatacgtt cagcgggtcg gtgtaaatat tccgtagctg aatagactct
    25321 gcaatccacg gcagatcggc catcagatgg gaatcgttgg caatcgccag caccactttg
    25381 atgtcttctt cttgcaggtt gcgtaactct ttacctaacg gccacagtgc tttgtctacc
    25441 agacgttgat catagtattc cgccaaccac aggtctgctt tggcgaagac catctccagc
    25501 atgccgagac gcgtcgagaa gaatggccaa tcgcggcaca tggcttccag ctcgctctgt
    25561 ttgccatctt cgaccacttt ttgcagcgcc gtacctgcac ccagccaggc ggggagcatc
    25621 aggcggtttt gcgtccaggc aaaaatccac ggtatggcgc gcagcgactc gacgccgccg
    25681 gttgggcgac gtttcgccgg acgtgaaccc aacggcagtt tgcccaattc ttgttccggc
    25741 gtagcggagc ggaagtaagg cacaaaatct ttgttttcac gtacgtagcc acggtagaga
    25801 tcgcaggaga tgactgacag ttcatccata atgcgacgcc agctctcttt cggctcaggt
    25861 ggtggcagca ggttggcttc cagaatcgcc ccggtgtaaa gcgacaggct gctgacggtg
    25921 atttctggca gaccatattt aaagcggatc atctcgccct gttcagtcac gcgcaggcca
    25981 cctttcaggc ttcctggcgg ttgtgacagc agcgccgcat gggcaggtgc gccgccgcga
    26041 ccaatggaac cgccgcgacc gtggaacaac gtcagctcaa tacccgcttt ttcgcaggtt
    26101 ttgattaatg catcctgtgc ctgatattgc gcccaggaag ctgccatcac gcccgcatct
    26161 tttgctgagt cggaatagcc aatcatcacc atctgtttgc cctgaatcag gccgcgatac
    26221 cagtcgatat tgagcagctg ggtcatgaca tcgttggcgt tgttcaggtc atcgagggtt
    26281 tcaaacagcg gagcaaccgg catcgcaaac ccgatacccg cttctttcag cagcaggtgg
    26341 acagccagta cgtcggacgg cgttttcgcc atcgagatca cgtaggcggc aatggagcct
    26401 tgcggcgctt cggcaatcac ctggcaggta tcgagcactt cgcgcgtttc ggcgcttggt
    26461 tgccagttgc gcggcagaag cggacgtttg gagttcagtt cgcggatcag gaacgcctgt
    26521 ttgtcggcct ctgaccagct ttcgtagtcg ccgataccga ggtagcgggt cagctcgccc
    26581 agcgcttcgg tatggcgggt gctctcctga cggatatcaa tacggaccag cggtacgccg
    26641 aaacatttca cgcggcgcag ggtgtcgagc agatcgccgt tggcgataat acccatgcca
    26701 cacgcctgaa gtgactggta gcaagcgtag agcggttccc acagttcttc gttttgtgtc
    26761 agcagccctt ctggttttgg cagttcttcg cctttcaggc gcgcttccag ccatgcctgt
    26821 gtcgccatca ggcgagaacg caggtttttc atcagatagc gatacggttc tgcggcacct
    26881 tcttcgccaa ccagcgccag cagttcaggg gtcgcttcaa ccatcgacag ttcagaaacc
    26941 agaacctgaa tatctttcag gaacagatcg gtggctttcc agcgactgag tagcaggacg
    27001 tggcgggtga tatcggcagt gacgttcggg ttgccgtcgc ggtcaccgcc catccacgaa
    27061 gtaaaacgga ccggaacaaa ttcgacgggc agtttgtagc cgaggttctc ttccagttgt
    27121 tcgttcagtt cgcgcaagta attcggtacg ccttgccaca ggctgttttc tactacggca
    27181 aagccccatt tggcttcatc taccgggctt ggacgcagct tacggatttc atcagtatgc
    27241 catgactggg cgattaactg gcgcaggcga cgcatcagct ggttgtgttc gtagtcagcg
    27301 atatctttgt tatcgagctg ttttaagcag gcgttcactt ccaccatttt gtggatcagt
    27361 gtacgacggg taatttcggt tgggtgagcc gtgaggacca gttccagcga cagcgattcc
    27421 actgcttttt tgatggtgtc ttcgctcagt tccggctggt ttttcagttt gcgcagggtg
    27481 cgggcgatca cttccgggtt gctggcagct tcgcctttcg gcgaaatgct gtggtattgc
    27541 tcggcggtgt tggccaggtt caggaactga ctaaacgcac gcgcaacggg cagcagctcg
    27601 tcgttcgaca aattttgtaa ggtggtgagc aactcctggc ggttagcatc attgccagcg
    27661 cgtgaagatt tcgacaactt acggatagtt tctacgcgtt caagaatgtg ttctcccaac
    27721 gcatccttga tggtttctcc cagcactttg ccgagcatac tgacattact acgcaatgcg
    27781 gaatattgtt cgttcatatt accccagaca ccccatctta tcgtttgata gccctgtatc
    27841 cttcacgtcg cattggcgcg aatatgctcg ggctttgctt ttcgtcgtct tttataaagc
    27901 cacgtaaaag cggtgacgtc aaatgctgcg aaatcgcttc agcaaacgaa taaatagcag
    27961 gaatttacgc cattaaattc acgacgcttt aaataagcgt aacttatgga aatgttaaaa
    28021 aatcgccata cataacacca aaggtgtagg tcggataaga tgcgcaagca tcgcatccga
    28081 cattattgcg gcaccggagt ttggcaacgg tgccggatgc ggcgcgagcg ccttatccgg
    28141 cctacgtttt aatgccagca aaaatggtga attacctgga taatcaactc acgggtgggt
    28201 ttaataaacc gcgtctccag atattcatca ggctgatgag cttgattgat ggaacccgga
    28261 ccgagcacca gcgtcgggca taacgtttga ataaacggcg cttcggtaca gtaattcacc
    28321 acttcggttt ttgctccgag caatttctca accacttcaa ccagttgatg attcggtggg
    28381 cattcatagc cagggatcgg cggatgcagc tcgtcgaccg tcagacgacc cggccagcgt
    28441 tcgctcaccg gagccaatgc atcgttgagc aaaccattaa gttcattgag tgtcatgcta
    28501 ggcagcggac gaatatccat atgcaactca cagcaagcgc aaatacggtt agaagcgtcg
    28561 ccaccgtgaa tatgcccgag gttgagcgta gggtatggca cggtaaatgc ttcgtagtga
    28621 taacgttctt tcaggttatc gcgcaattgc aaaatatgcc cgatggcgtc gtgcattagt
    28681 tcgatagcgt taactccgcg tgctggatcg ctggagtgcc ccgactggcc ctgaatacgg
    28741 atggcgttag agatatgacc tttatgtgcg cgtaccggtt gtagtgacgt cggttcgcca
    28801 atgatggcgc aatccgggcg cagggcggta gtttcggcaa aataacgcgc tccggccata
    28861 ctggtttctt catcagcagt cgccagaatg tagagcggtt ttttcagttt cgtgacgtcg
    28921 acatcgcgta gcgtatcaag gataaacgca aaaaagcctt tcatgtcggc ggtgcctaag
    28981 ccgtaaagct tgccgtcatg ctccgtcagt gtaaacggat cgcgcgtcca gcgaccgtca
    29041 tcaaatggca ccgtatcggt atgccccgcc agcaacaagc cgccagcccc ctgtccgata
    29101 cttgccagca tattgaattt gttgcgagtt cctggcacag gctgcacttc cacattgaag
    29161 cctaaatctt taaaccagtc cgccagcaga gtgattaaat ctgcattgct ttgatcgagt
    29221 gcctcttccg tggcgcttat tgaaggtgtg gcaatcagag cacggtaaat ctcgataaat
    29281 ggcggtaatt tgtttttcat tgttgacaca cctctggtca tgatagtatc aatattcatg
    29341 cagtatttat gaataaaaat acactaacgt tgagcgtaat aaaacccacc agccgtaagg
    29401 tgaatgtttt acgtttaacc tggcaaccag acataagaag gtgaatagcc ccgatgttga
    29461 atacgctgat tgtgggtgcc agcggctacg ctggcgcaga gctagtgacc tatgtaaatc
    29521 gccatccgca tatgaacata accgctttga ctgtttcagc gcaaagcaat gatgcgggaa
    29581 agttaatctc cgatttgcat ccgcagctaa aaggcatcgt tgagctgccg ttgcagccga
    29641 tgtcggatat cagcgagttt agcccagggg tggacgtagt gtttctcgcc accgcccatg
    29701 aagttagcca cgatttagcg ccgcagtttc ttgaagcggg ctgcgtggtg ttcgaccttt
    29761 ccggtgcgtt tcgtgttaac gacgccacct tctatgaaaa atattacggc tttacccatc
    29821 aatacccgga actgctggaa caggcagcct acggtctggc ggagtggtgc ggtaataaat
    29881 taaaagaagc gaatttgatt gcggtgccag gctgttatcc gacggcggca cagctggcgc
    29941 tgaaaccgtt gattgatgcc gatcttcttg acctcaatca gtggccggtg atcaacgcca
    30001 ccagcggcgt gagcggtgca ggacgtaagg cagcgatttc aaatagtttt tgtgaagtta
    30061 gcctgcaacc gtatggcgtc tttactcatc gccatcaacc agagatcgcc acacacctcg
    30121 gtgctgacgt tatcttcacc ccacatctgg gcaatttccc gcgcggcatt ctcgaaacca
    30181 ttacctgccg cctgaaatcg ggtgtgaccc aggcgcaagt cgcgcaagtg ttacagcagg
    30241 cgtatgccca taaaccgctg gtgcggctgt atgacaaagg cgttccggcg ctgaaaaatg
    30301 tcgttgggct gccattttgc gatatcgggt ttgccgttca gggcgagcat ctgattattg
    30361 tggcgaccga agacaactta ctgaaaggcg cggcggcaca agcggtacag tgcgccaata
    30421 ttcgtttcgg ctatgcggaa acgcagtctc ttatttaagg gtgcaatgat gaatccatta
    30481 attatcaaac tgggcggcgt actgctggat agtgaagagg cgctggaacg tctgtttagc
    30541 acactggtga attatcgtga gtcacatcag cgtccgctgg tgattgtgca cggcggcggt
    30601 tgcgtggtgg atgagctgat gaaagggctg aatctgccgg tgaaaaagaa aaacggcctg
    30661 cgggtgacgc ctgctgatca gatagacatt atcaccggag cactggcggg aacggcaaat
    30721 aaaaccctgt tggcatgggc gaagaaacat cagattgcgg ccgtaggttt gtttctcggt
    30781 gacggcgaca gcgtcaaagt gacccagctt gatgaagagt taggtcatgt tggactggcg
    30841 cagccaggtt cgcctaagct tatcaactcc ttgctggaga acggttatct gccggtggtc
    30901 agctccattg gcgtaacaga cgaagggcaa ctgatgaacg tcaatgccga ccaggcggca
    30961 acggcgctgg cggcaacgct gggcgcggat ctgattttgc tctccgacgt cagcggcatt
    31021 ctcgacggca aagggcaacg cattgccgaa atgaccgccg cgaaagcaga acaactgatt
    31081 gagcagggca ttattactga cggcatgata gtgaaagtga acgcggcgct ggatgcggcc
    31141 cgcacgctgg gccgtccggt agatatcgcc tcctggcgtc atgcggagca gcttccggca
    31201 ctgtttaacg gtatgccgat gggtacgcgg attttagctt aagttttgtt ggccggaggc
    31261 gcagctttcc ggcattgaat ttcaaaataa ggaaacagag ttatggcact ttggggcggg
    31321 cgttttaccc aggcagcaga tcaacggttc aaacaattca acgactcact gcgctttgat
    31381 taccgtctgg cggagcagga tattgttggc tctgtggcct ggtccaaagc cctggtcacg
    31441 gtaggcgtgt taaccgcaga agagcaggcg caactggaag aggcgctgag cgtgctgctg
    31501 gaagatgttc gcgccaggcc acaacaaatc cttgaaagcg acgccgaaga tatccatagc
    31561 tgggtggaag gcaaactgat cgacaaagtg ggccagttag gcaaaaagct gcataccggg
    31621 cgtagtcgta acgatcaggt agcgactgac ctgaaactgt ggtgcaaaga taccgttagc
    31681 gagttactga cggctaaccg acagctgcaa tcggcgctgg tggaaaccgc acaaaacaat
    31741 caggacgcgg tcatgccagg ttacactcac ctgcaacgcg cccagccggt gacgttcgcg
    31801 cactggtgcc tggcctatgt tgagatgctg gcgcgtgatg aaagccgttt gcaggatgcg
    31861 cttaagcgtc tggatgtcag cccattaggc tgtggcgcgc tggcgggaac ggcctatgaa
    31921 atcgaccgtg aacagttagc aggctggctg ggctttgctt cggcgacccg taacagtctg
    31981 gacagcgttt ctgaccgtga ccatgtgttg gaactgctgt cggctgccgc tatcggcatg
    32041 gtgcatctgt cgcgttttgc tgaagatctg attttcttta acaccggcga agcggggttt
    32101 gtggagcttt ctgaccgcgt gacttccggt tcatcattaa tgccgcagaa gaaaaacccg
    32161 gatgcgctgg agctgattcg cggtaaatgc ggtcgggtgc agggtgcgtt aaccggcatg
    32221 atgatgacgc tgaaaggttt gccgctggct tacaacaaag atatgcagga agacaaagaa
    32281 ggtctgttcg acgcgctcga tacctggctg gactgcctgc atatggcggc gctggtgctg
    32341 gacggcattc aggtgaaacg tccacgttgc caggaagcgg ctcagcaggg ttacgccaac
    32401 gccaccgaac tggcggatta cctggtggcg aaaggcgtac ctttccgcga ggcgcaccat
    32461 atcgtgggtg aagcggtggt ggaagccatt cgtcagggca aaccgctgga agatctgccg
    32521 ctcgacgagt tgcagaaatt cagcccggtg attggcgaag atgtctatcc gattttgtcg
    32581 ctgcaatcgt gcctcgacaa gcgtgcggca aaaggcggcg tctcaccgca gcaggtggcc
    32641 caagcgattg cttttgcgca ggcgcggttg gggtaagaac atttatatgt acaaatttga
    32701 gcctggctta tcgccgggct tttttatggc aaaaaaaagc ggatcctgga gatccgcaaa
    32761 agttcacgtt ggctttagtt attcgagttg agaaactctc gaaacgggca gtgacttcaa
    32821 gggttaaaag aggtgccgct ccgtttctgt gagcaattat cagtcagaat gcttgatagg
    32881 gataatcgtt cattgctatt ctacctatcg ccatgaacta tcgtggcgat ggaggatgga
    32941 taatgaatat tcgtgatctt gagtacctgg tggcattggc tgaacaccgc cattttcggc
    33001 gtgcggcaga ttcctgccac gttagccagc cgacgcttag cgggcaaatt cgtaagctgg
    33061 aagatgagct gggcgtgatg ttgctggagc ggaccagccg taaagtgttg ttcacccagg
    33121 cgggaatgct gctggtggat caggcgcgta ccgtgctgcg tgaggtgaaa gtccttaaag
    33181 agatggcaag ccagcagggc gagacgatgt ccggaccgct gcatattggt ttgattccca
    33241 cggttggacc gtacctgcta ccgcatatta tcccgatgct gcaccagacc tttccaaagc
    33301 tggaaatgta tctgcatgaa gcacagaccc accagttact ggcgcaactg gacagtggca
    33361 aactcgattg cgtgatcctc gcgctggtga aagagagcga agcattcatt gaagtgccgt
    33421 tgtttgatga gccaatgttg ctggctatct atgaagatca cccgtgggcg aaccgcgaat
    33481 gcgtaccgat ggccgatctg gcaggggaaa aactgctgat gctggaagat ggtcactgtt
    33541 tgcgcgatca ggcaatgggt ttctgttttg aagccggggc ggatgaagat acacacttcc
    33601 gcgcgaccag cctggagacg ctgcgcaaca tggtggcggc aggtagcggg atcactttac
    33661 tgccagcgct ggctgtgccg ccggagcgca aacgcgatgg ggttgtttat ctgccgtgca
    33721 ttaagccgga accacgccgc actattggcc tggtttatcg tcctggctca ccgctgcgca
    33781 gccgctatga gcagctggca gaggccatcc gagcaagaat ggatggctat ttcgataaag
    33841 tgttaaaaca ggcggtttaa accgtttaac gcagctaccc gataggcttc cgccatggtc
    33901 gggtagttaa aggtggtgtt gacgaagtac tcaatagtgt tgccgccacc tttctgttcc
    33961 ataatcgcct gaccgatatg aataatttcg gcagcgcgct cgccaaagca gtgaataccc
    34021 agaatctctt ttgtttcccg atggaacaaa attttcagcg tgcccacgtt catgccgacg
    34081 atttgtgcgc gtgccagatg tttaaactgg gcgcggccca cttcatatgg cactttcatc
    34141 gcggtcagct gctgttcggt tttgcccaca gagctgattt ccgggatggt gtaaatgccg
    34201 gtagggatat cttcaatcag atgcgcggtg gcttcgcctt ttaccagcgc ctgcgcggca
    34261 atgcgcccct ggtcataggc cgctgatgcc aggctcggat aaccaatcac gtcgcccacc
    34321 gcgtaaacgt gcggctgtgc ggtctgatac atgctgttga ccttcagctg tccgcgactg
    34381 tcagtttcca gcccaatgtt ctgtaacgcc agcgaatcgg tattaccggt acgaccgttg
    34441 gcatagagca ggcagtcagc tttcagtttt ttacccgact tcagatgcat gatcacaccg
    34501 tcgtcacagc cttcgatctt ctcgtactct tcgttgtgac gaatcactac gccactgttc
    34561 cagaagtgat aggagagaga atctgacatc tcttgatcga gaaatgccag caggcgatcg
    34621 cgggtgttga tcagatccac ttttacatcc ataccgcgga agatcgacgc atattcacag
    34681 ccgatcactc cagcaccata gataagtaca tggcgcggtt cgtggtgcat gctaagaatt
    34741 gagtcgctgt cgtaaatgcg tggatgggtg aaatcaacat ctgttggatg atatggacga
    34801 gagccgcagg caataacaaa tttttcagcg gttagtgttt cgacgctgcc gtccgggcaa
    34861 tccagcgcca acgtatgctc gtcaacaaag cgagcgttcc cctgcaatat ttcacagtga
    34921 ttacgttcgt aaaatccctg acgcatgcgc gtttgttgat taatcacgtt atcggcatgg
    34981 ttaaggatat cggcaaaaga agagcggagc agtcgcgaat ggtcgctgta tagtgggttt
    35041 tgattgaatt ctataatgcg gctgacggcg tgacggagag ctttcgacgg gatggtgccc
    35101 cagtgggtgc aaccgccgcc aacattttga taacgctcga taactgcgac gcgcacacct
    35161 tgcttaacca ggcccattgc agcgccttcg ccgccggggc cggaacctat tactatggca
    35221 tcgtaatcgt aggaatgtgg catggtaggg cttacctgtt cttatacata aaagcaacag
    35281 aatggtaaca ttctatcgcg ggtaagccaa ttgatccccg tcatttatct ggctatatcc
    35341 tgagcggcct ttgctttgtc tgtttcttac ttttgccctg acgttttatt ggatttttat
    35401 cgacgatact ctccgtttaa gcggcaggtt tccgctgtac gtaaaagaac cggccagtaa
    35461 atatgtttta ttgcgttacc gttcattcac aatactggag caatccagta tgttcattct
    35521 ctggtatagt gccagcagta cttttggcaa ggattcagac atcgtgatgg gcataagagc
    35581 gcaacaaaaa gaaaaaaccc gccgttcgct ggtggaagcc gcatttagcc aattaagtgc
    35641 tgaacgcagc ttcgccagcc tgagtttgcg tgaagtggcg cgtgaagcgg gcattgctcc
    35701 cacctctttt tatcggcatt tccgcgacgt agacgaactg ggtctgacca tggttgatga
    35761 gagcggttta atgctacgcc aactcatgcg ccaggcgcgt cagcgtatcg ccaaaggcgg
    35821 gagtgtgatc cgcacctcgg tctccacatt tatggagttc atcggtaata atcctaacgc
    35881 cttccggtta ttattgcggg aacgctccgg cacctccgct gcgtttcgtg ccgccgttgc
    35941 gcgtgaaatt cagcacttca ttgcggaact tgcggactat ctggaactcg aaaaccatat
    36001 gccgcgtgcg tttactgaag cgcaagccga agcaatggtg acaattgtct tcagtgcggg
    36061 tgccgaggcg ttggacgtcg gcgtcgaaca acgtcggcaa ttagaagagc gactggtact
    36121 gcaactgcga atgatttcga aaggggctta ttactggtat cgccgtgaac aagagaaaac
    36181 cgcaattatt ccgggaaatg tgaaggacga gtaatgaaac aagcaaatca agatagaggt
    36241 acgctgctgc tggcgttagt tgctggctta tcgattaatg gtactttcgc agcgctgttt
    36301 agctccattg tgccattttc tgtattcccg attatttccc tggtgctgac ggtttactgc
    36361 ctgcatcaac gttatcttaa tcgcactatg ccggtaggct tgccgggtct ggcagctgcc
    36421 tgttttattc tcggcgtact gctgtacagc acggtagttc gtgcggaata tccggatatc
    36481 ggctctaact tcttcccggc agtactctcc gtcattatgg tgttctggat tggcgcgaag
    36541 atgcgtaacc gtaagcagga agttgctgag taatcggtat tatgccggat gctggcgcat
    36601 ccggcatggg ttttacttcg cggtcagtaa tacgccgcac tccatatggt gcgtgtaggg
    36661 gaactgatca aacagagcca gacgttcgac cttgtgcgtc tggcttaatg tttccagatt
    36721 cttgcataac gtttccgggt tacaggagat gtacaaaata cacggatacg cctgcaccat
    36781 tttctcggtt tcactgtcca gaccgctgcg cggtgggtcg acaaaaatgg tttcgcactg
    36841 ataactcttt aagtcgatcc cttgcaggcg gttaaactcg cgcacaccat tcatcgcctg
    36901 agtaaattct tctgccgcca tacgaataat ttgtacgtta tcaatatggt tagctgcgat
    36961 gttgtattgc gcagcagcaa ccgacggctt agcgatttcg gtggctaata cccgatcaaa
    37021 attgcgcgcc agcgctaatg aaaagttacc gttgccgcag tacagctcca gtaaatcgcc
    37081 ttttgagcct ttggttacgt ccagcgccca ttccagcatc tgaatattca tcgccgcgtt
    37141 cggctgggta aagctgtttt ctacctgacg gtagatcatc tctttccctg cgaccggcag
    37201 acgctcgtcg atgtaatctt gatccagctc gattttggtt ttcgttgccc gaccaatcag
    37261 atgcacattc agattctgcg cgcgcagtgc atcgcgcagg gcctccgctt cctgacgcca
    37321 ctcatcatcc agcttcttat ggtatagcag ggaaaccacc gcctgattac tcagcgtggt
    37381 gaggtaatca atctggaaca acttgtggcg cagaacggga ttattacgca cacccgcaat
    37441 catcgccgtc atcaactggt tgataagttc actggcggcg gggaagctat ccacgcggat
    37501 acggcttttg gtttgttgat cgaaaatgat gtgatacagg tcatcgccat cgtgccagat
    37561 gcggaactcc gcacgcatcc ggtaatgact gaccggcgag cgaaacactt ccggaaccag
    37621 gtcagaaaac ggtgccatca tactttgcaa acgtaccact ttttcggcta actgcgcttc
    37681 atactgttct gttggaaggt gttcgggggt catgatgtat cctgaaaaat taaagtacgc
    37741 ggcgattgta gggattgctc atcagatgtc cagatcttga tgaattccta tttgtgagct
    37801 acgtctggac agtaacttgt tacaacctgt agcatccact tgtcggtcct gtgagttaat
    37861 agggaatcca gtgcgaatct ggagctgacg cgcagcggta aggaaaggtg cgatgattgc
    37921 gttatgcgga cactgccatc cggtgggaag tcatcatctc ttagtatctt agatacccct
    37981 ccaagcccga agacctgccg gccaacgtcg catctggttc tcatcatcgc gtaatattga
    38041 tgaaacctgc ggcatccttc ttctattgtg gatgctttac aatgattaaa aaagcttcgc
    38101 tgctgacggc gtgttctgtc acagcctttt ccgcttgggc acaggatacc agcccggata
    38161 ctctcgtcgt tactgctaac cgttttgaac agccgcgcag cactgtgctt gcaccaacca
    38221 ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac ctcggtcaat gatgtgctgc
    38281 gccgtcttcc gggcgtcgat atcacccaaa acggcggttc aggtcagctc tcatctattt
    38341 ttattcgcgg tacaaatgcc agtcatgtgt tggtgttaat tgatggcgta cgcctgaatc
    38401 tggcgggggt gagtggttct gccgacctta gccagttccc tattgcgctt gtccagcgtg
    38461 ttgaatatat ccgtgggccg cgctccgctg tttatggttc cgatgcaata ggcggggtgg
    38521 tgaatatcat cacgacgcgc gatgaacccg gaacggaaat ttcagcaggg tggggaagca
    38581 atagttatca gaactatgat gtctctacgc agcaacaact gggggataag acacggggaa
    38641 cgctgttggg cgattatgcc catactcatg gttatgatgt tgttgcctat ggtaatacag
    38701 gaacgcaagc gcagacagat aacgatggtt ttttaagtaa aacgctttat ggcgcgctgg
    38761 agcataactt tactgatgcc tggagcggct ttgtgcgcgg ctatggctat gataaccgta
    38821 ccaattatga cgcgtattat tctcccggtt caccgttgct cgatacccgt aaactctata
    38881 gccaaagttg ggacgccggg ctgcgctata acggcgaact gattaaatca caactcatta
    38941 ccagctatag ccatagcaaa gattacaact acgatcccca ttatggtcgt tatgattcgt
    39001 cggcgacgct cgatgagatg aagcaataca ccgtccagtg ggcaaacaat gtcatcgttg
    39061 gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca gactacgacg ccgggtacag
    39121 gttatgttga ggatggatat gatcaacgta ataccggcat ctatctgacc gggctgcaac
    39181 aagtcggcga ttttaccttt gaaggcgcag cacgcagtga cgataactca cagtttggtc
    39241 gtcatggaac ctggcaaacc agcgccggtt gggaattcat cgaaggttat cgcttcattg
    39301 cttcctacgg gacatcttat aaggcaccaa atctggggca actgtatggc ttctacggaa
    39361 atccgaatct ggacccggag aaaagcaaac agtgggaagg cgcgtttgaa ggcttaaccg
    39421 ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt cagtgacttg atcgattatg
    39481 atgatcacac cctgaaatat tacaacgaag ggaaagcgcg gattaagggc gtcgaggcga
    39541 ccgccaattt tgataccgga ccactgacgc atactgtgag ttatgattat gtcgatgcgc
    39601 gcaatgcgat taccgacacg ccgttgttac gccgtgctaa acagcaggtg aaataccagc
    39661 tcgactggca gttgtatgac ttcgactggg gtattactta tcagtattta ggcactcgct
    39721 atgataagga ttactcatct tatccttatc aaaccgttaa aatgggcggt gtgagcttgt
    39781 gggatcttgc ggttgcgtat ccggtcacct ctcacctgac agttcgtggt aaaatagcca
    39841 acctgttcga caaagattat gagacagtct atggctacca aactgcagga cgggaataca
    39901 ccttgtctgg cagctacacc ttctgaacca cgtcccaccg tgctggtgtt tgactccggc
    39961 gtcggtgggt tgtcggtcta tgacgagatc cggcatctct taccggatct ccattacatt
    40021 tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa gcgaagcgtt tattgttgag
    40081 cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc cccttgcgct ggctgtggtc
    40141 gcttgcaaca ctgccagtac cgtttcactt cctgcattac gcgaaaagtt cgacttcccg
    40201 gttgttggtg tcgtgccggc gattaaacct gctgcacgtc tgacggcaaa tggcattgtc
    40261 ggattactgg caacccgcgg aacagttaaa cgttcttata ctcatgagct gatcgcgcgt
    40321 ttcgctaatg aatgccagat agaaatgctg ggctcggcag agatggttga gttggctgaa
    40381 gcgaagctac atggcgaaga tgtttctctg gatgcactaa aacgtatcct acgcccgtgg
    40441 ttaagaatga aagagccgcc agataccgtt gtattgggtt gcacccattt ccctctacta
    40501 caagaagaac tgttacaagt gctgccagag ggaacccggc tggtggattc tggcgcagcg
    40561 attgctcgcc gaacggcctg gttgttagaa catgaagccc cggatgcaaa atctgccgat
    40621 gcgaatattg ccttttgtat ggcaatgacg ccagaagctg aacaattatt gcccgtttta
    40681 cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt taggctgatt tggttgaatg
    40741 ttgcgcggtc agaaaattat tttaaatttc ctcttgtcag gccggaataa ctccctataa
    40801 tgcgccacca ctgacacgga acaacggcaa
//
LOCUS       DAJTVJ010000021        39858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000021 DAJTVJ010000000
VERSION     DAJTVJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.021"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            5..436
                     /locus_tag="OO448_001099"
     CDS             5..436
                     /locus_tag="OO448_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6336368.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="OO448_001100"
     CDS             519..1421
                     /locus_tag="OO448_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR6336369.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="OO448_001101"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="OO448_001101"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6336370.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="OO448_001102"
     CDS             complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="OO448_001102"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6336371.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="OO448_001103"
     CDS             complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="OO448_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6336372.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5331..6038)
                     /locus_tag="OO448_001104"
     CDS             complement(5331..6038)
                     /locus_tag="OO448_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6336373.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="OO448_001105"
     CDS             complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="OO448_001105"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6336374.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7099)
                     /locus_tag="OO448_001106"
     CDS             complement(6896..7099)
                     /locus_tag="OO448_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6336375.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7667..7987
                     /gene="cyaY"
                     /locus_tag="OO448_001107"
     CDS             7667..7987
                     /gene="cyaY"
                     /locus_tag="OO448_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6336376.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8027..10573)
                     /gene="cyaA"
                     /locus_tag="OO448_001108"
     CDS             complement(8027..10573)
                     /gene="cyaA"
                     /locus_tag="OO448_001108"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6336377.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10960..11901
                     /gene="hemC"
                     /locus_tag="OO448_001109"
     CDS             10960..11901
                     /gene="hemC"
                     /locus_tag="OO448_001109"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6336378.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11898..12638
                     /gene="hemD"
                     /locus_tag="OO448_001110"
     CDS             11898..12638
                     /gene="hemD"
                     /locus_tag="OO448_001110"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6336379.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12660..13883
                     /gene="hemX"
                     /locus_tag="OO448_001111"
     CDS             12660..13883
                     /gene="hemX"
                     /locus_tag="OO448_001111"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6336380.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            13886..15082
                     /gene="hemY"
                     /locus_tag="OO448_001112"
     CDS             13886..15082
                     /gene="hemY"
                     /locus_tag="OO448_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6336381.1"
                     /translation="MLKVLLLFVLLIAGVVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15744..15905
                     /locus_tag="OO448_001113"
     CDS             15744..15905
                     /locus_tag="OO448_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336382.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15989..17224)
                     /locus_tag="OO448_001114"
     CDS             complement(15989..17224)
                     /locus_tag="OO448_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6336383.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17371..17447)
                     /locus_tag="OO448_001115"
     tRNA            complement(17371..17447)
                     /locus_tag="OO448_001115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17411..17413),aa:Pro,seq:tgg)
     gene            complement(17490..17576)
                     /locus_tag="OO448_001116"
     tRNA            complement(17490..17576)
                     /locus_tag="OO448_001116"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17540..17542),aa:Leu,seq:cag)
     gene            complement(17597..17672)
                     /locus_tag="OO448_001117"
     tRNA            complement(17597..17672)
                     /locus_tag="OO448_001117"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17637..17639),aa:His,seq:gtg)
     gene            complement(17731..17807)
                     /locus_tag="OO448_001118"
     tRNA            complement(17731..17807)
                     /locus_tag="OO448_001118"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17771..17773),aa:Arg,seq:ccg)
     gene            complement(17908..19293)
                     /locus_tag="OO448_001119"
     CDS             complement(17908..19293)
                     /locus_tag="OO448_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6336384.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19484..20224)
                     /gene="rffM"
                     /locus_tag="OO448_001120"
     CDS             complement(19484..20224)
                     /gene="rffM"
                     /locus_tag="OO448_001120"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6336385.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20227..21579)
                     /gene="wzyE"
                     /locus_tag="OO448_001121"
     CDS             complement(20227..21579)
                     /gene="wzyE"
                     /locus_tag="OO448_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6336386.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21576..22655)
                     /gene="rffT"
                     /locus_tag="OO448_001122"
                     /gene_synonym="wecF"
     CDS             complement(21576..22655)
                     /gene="rffT"
                     /locus_tag="OO448_001122"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6336387.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22652..23902)
                     /gene="wzxE"
                     /locus_tag="OO448_001123"
     CDS             complement(22652..23902)
                     /gene="wzxE"
                     /locus_tag="OO448_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6336388.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23904..25034)
                     /gene="rffA"
                     /locus_tag="OO448_001124"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23904..25034)
                     /gene="rffA"
                     /locus_tag="OO448_001124"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6336389.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(25039..25713)
                     /gene="rffC"
                     /locus_tag="OO448_001125"
                     /gene_synonym="wecD"
     CDS             complement(25039..25713)
                     /gene="rffC"
                     /locus_tag="OO448_001125"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6336390.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25691..26572)
                     /gene="rfbA"
                     /locus_tag="OO448_001126"
     CDS             complement(25691..26572)
                     /gene="rfbA"
                     /locus_tag="OO448_001126"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6336391.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26591..27658)
                     /gene="rffG"
                     /locus_tag="OO448_001127"
     CDS             complement(26591..27658)
                     /gene="rffG"
                     /locus_tag="OO448_001127"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6336392.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27658..28920)
                     /gene="wecC"
                     /locus_tag="OO448_001128"
     CDS             complement(27658..28920)
                     /gene="wecC"
                     /locus_tag="OO448_001128"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6336393.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28917..30047)
                     /gene="wecB"
                     /locus_tag="OO448_001129"
     CDS             complement(28917..30047)
                     /gene="wecB"
                     /locus_tag="OO448_001129"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6336394.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30103..31149)
                     /gene="wzzE"
                     /locus_tag="OO448_001130"
     CDS             complement(30103..31149)
                     /gene="wzzE"
                     /locus_tag="OO448_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6336395.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31161..32264)
                     /gene="wecA"
                     /locus_tag="OO448_001131"
     CDS             complement(31161..32264)
                     /gene="wecA"
                     /locus_tag="OO448_001131"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6336396.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32504..33763)
                     /gene="rho"
                     /locus_tag="OO448_001132"
     CDS             complement(32504..33763)
                     /gene="rho"
                     /locus_tag="OO448_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6336397.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33848..33949)
                     /gene="rhoL"
                     /locus_tag="OO448_001133"
     CDS             complement(33848..33949)
                     /gene="rhoL"
                     /locus_tag="OO448_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6336398.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34090..34419)
                     /gene="trxA"
                     /locus_tag="OO448_001134"
     CDS             complement(34090..34419)
                     /gene="trxA"
                     /locus_tag="OO448_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6336399.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34550..35815
                     /gene="rhlB"
                     /locus_tag="OO448_001135"
     CDS             34550..35815
                     /gene="rhlB"
                     /locus_tag="OO448_001135"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6336400.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35949..37433
                     /gene="gppA"
                     /locus_tag="OO448_001136"
     CDS             35949..37433
                     /gene="gppA"
                     /locus_tag="OO448_001136"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6336401.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37480..39501)
                     /gene="rep"
                     /locus_tag="OO448_001137"
     CDS             complement(37480..39501)
                     /gene="rep"
                     /locus_tag="OO448_001137"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6336402.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9867 a        10840 c        10054 g         9097 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcgacat cgttcgtctg gatgccttca aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagaggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctgaaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggtg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagaccgcca gttactggcc gcaaggttcg gtatttgtca
      781 gtgtggtcga tcctggtgtt ggtacagacc gtaaatcggt agtgctaaaa accaaaaacg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgcct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgcctggct tccggagcga
     1021 tcacgttcga acaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtaaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatcta aacagcttat
     1321 tgaatgtttc cgtggcgctc aatatggata atttcgcgca gaaacatcaa gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcaggatgcg
     1441 gtgtggcgca ccgcatcctg caacggagtt gtaaattaca ccgtctccag ccgggcgtaa
     1501 gccgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg gctatgttca
     1561 ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg cacgcgctgg
     1621 ccgagtttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctaacc
     1681 gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg
     1741 ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag
     1801 gtcagcgtca gtttctgcat cgcacgggtt acgccaacgt aggccagacg gcgttcttct
     1861 tccagacgtc cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg
     1921 acgataaaca cctgcgggaa ctccagccct ttcgccgagt gtagcgtcat caactgcacc
     1981 gcatcctgcc aagtatccgc ctgcccttca cctgcttcca gtgccgcatg ggagaggaac
     2041 gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc
     2101 accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac
     2161 atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg
     2221 gcagtttctt gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct
     2281 cgcccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg
     2341 cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc
     2401 gtattcacca cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac
     2461 gagagcgcgt ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac
     2521 ggcatactgg cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag
     2581 agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aagttttgat gcggttaacc
     2641 acaaaacgcg cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc
     2701 gcgccatcgg tccacagttt tttacccaga cgcccgttgt tgttttcaat cagggcgttc
     2761 gcggcgctca gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca
     2821 ccggggaaat cattgaggaa acgctgaata ttctctacct gcgccccgcg ccagccgtag
     2881 attgactggt cgtcatcgcc aacgatcatc actttgccgg tatcgcccgc cagcaggcgg
     2941 atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat gttggtaaaa
     3001 cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc
     3061 agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata cgcctgatac
     3121 accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg
     3181 ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg
     3241 tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc actgtcgagg
     3301 atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa acggtgcgcc
     3361 agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata
     3421 cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat cgagtatggc
     3481 gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca
     3541 ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc ttcgcgctgt
     3601 ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag
     3661 gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttactg
     3721 attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc
     3781 tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc
     3841 tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga
     3901 tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc
     3961 catgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct
     4021 gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt
     4081 cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg
     4141 cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa
     4201 tcgaacgaaa acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg
     4261 cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt
     4321 gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg
     4381 tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta
     4441 tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc
     4501 aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc
     4561 acgaagatcg ccgctcgact ccagcatatg cgtggcgaac gagtgacgta atttatgcgg
     4621 atgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt
     4681 acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa
     4741 caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag
     4801 gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg
     4861 tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag
     4921 cattgcacgg tcgcgtacag cgaggggatc gttgatatca atgtccagca gccgattcat
     4981 atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt
     5041 cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc
     5101 agaaagccgt aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa
     5161 attgcgcacc atcgtcacat cacattgctg ccagctttgc aggccgtttt cgctggcaaa
     5221 attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg
     5281 ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt
     5341 tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt
     5401 ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca
     5461 gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc
     5521 accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga
     5581 atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca
     5641 cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc
     5701 gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc
     5761 tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc
     5821 gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc
     5881 aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta
     5941 cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc
     6001 gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga
     6061 catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa
     6121 gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta
     6181 ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat
     6241 aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct
     6301 ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga
     6361 catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg
     6421 tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg
     6481 cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg
     6541 tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg gtcagtcctt
     6601 tcagacgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc
     6661 catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca
     6721 ccagcagttg gtcaaaccct acccccaggt gccgatcagc caggcgacga atcagctccg
     6781 gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat
     6841 gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt
     6901 aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt
     6961 gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat
     7021 agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg
     7081 ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct gctttctgct
     7141 ttctgctttc tgctttctgc tttctgcttt ctgctttctg ctttctgctt tctgctttct
     7201 gctttctgct ttctgctttc tgctttctgc tttctgcttt ctatcatcgc aggagaagac
     7261 gtaaaagcaa gcgtttagcg actctttatg aagtctgaaa agtgaggaaa tgctgcgagt
     7321 ataacgctta ctcgtgggga tttatgtttt gctggtagtt tcgtgatcgg cacctcgcgc
     7381 caggatggct ctatgcacta tcgcaatgtg gtttgtttgt tgtctttttt agttccgtca
     7441 gcgtgaggat gctcgctcgg taaacctgat cataatgttt accagaaaca gggaaaggcg
     7501 tggtgtaact gcgccctaat gaagagacaa atctttcgct acacagagac atcattattc
     7561 cggtgacgga tgaatcgtgc cggaacgcag ccgcagaggt gcaaactttg aagccctgac
     7621 cccatgccgc tatactgccg ccatcactta aaaacaggat acaacaatga acgacagtga
     7681 atttcatcgc ctggctgatc aactctggct gaccattgaa gaacgcctgg acgactggga
     7741 tggcgacagc gatatcgact gcgaaatcaa cggcggcgtg ctgaccatta cctttgaaaa
     7801 cggcagcaaa atcattatca accgccagga accgctgcac caggtatggc tggcaaccaa
     7861 acaaggcggc taccattttg atctgaaagg cgatgagtgg atttgcgatc gcagcggcga
     7921 aaccttctgg gatttgctgg aacaggcggc gacgcagcag gcgggtgaaa cagtcagttt
     7981 ccgctaagat tgcatgccgg ataagcctcg ctttccggca cgttcatcac gaaaaatatt
     8041 gctgtaatag cggcgtatcg tgatcctgat tggcaggcgg catgttaccg atagattttg
     8101 tgcggaacgg aatcacctgt tcacgaccat caaccttcac aatctgatag aactgcggca
     8161 ggttgaagtt gatgaagctt gagccgtagg tgaagcggtc gtgcgatgac gagtagaagc
     8221 gactgacgtc acgcaccagt tcctctttgc tgccttcgca gtggtgatac acctcaaccc
     8281 ggttgctttc gtcaagaatg tagatattaa agccattctc gtcttgcgtt tcttcgaaga
     8341 agaactggat gatcccttca ctagcaaagc cgtccaccac cgccggtaat ttgacgtgat
     8401 tggtttcaac ctgcactgac aggccgtgca gtttgttatg cgaaatagcg ccataaaact
     8461 cgatggcgtt ctccagcttc tgtaccgata cattcaggcg ttcgaagaac aagccccagg
     8521 tttgaccaga aacgcgcagc gccttgaaac gcccggtttc ctggcgggtg ctggaaaggc
     8581 gcagttcaat acactcagaa accagttgct gcacgcgagt acggattaac ccgcgcagat
     8641 gctggctata acagaagact tccacgctat ctggcggtgc ggcgtcctga tgcattttgc
     8701 cgagaatagt tttcagggct tcgatcatcg attgctcgcc gttgaagtgc agcgtacgca
     8761 cttcgttcca cgagttgcgg tacagcaggt caacgctacc taccaggcaa ttttgattct
     8821 cgccaaagct gaagacatcc agcttacgga aatcaaaatg caccacctga ttgcggaacg
     8881 ccgctgtcgg gtcatattcc aggttaacga taatcgccag atggcggatc tcacacgggc
     8941 tgtagagcgc cttcggtgtc ggtgcaggta agcgcagcgg gaaatggtgc gacacgtcgg
     9001 cgaccatctc ctgcaactta ggcaaatcga caatgccgtt acctttaata tacaaacggg
     9061 tgcgcgaggt cagcagaccg ttaaaccatg cccacgccac cagtttattc aggtaacggt
     9121 tatattccag cggctgatgg ctgatgatcg actcaatatt tggcgcgcgg ttatacagat
     9181 accaacctga acggttagcc cggcccggcg gcacataaat aaaggtcaga ttcggttccg
     9241 agagatcggg tgaaatctgc gggtttacca gcgtcacttt acctggtaat gcttcaaacg
     9301 cggcatacag cttacgcgtc agcacgccga tatcctgcgg actggcggag acgctaaggt
     9361 tattgcgacg cgcaaagcgg atcagattac ggtagctctg catcatcgcg tcgagcaact
     9421 cgttgtgcgc ctcacgcacc tgatcaatct tccagttagc acggttatcg agcattgcca
     9481 gacgagcttc gtcccaaccc cactcgctca ctaactggct caacactgcg cgacgccagc
     9541 ctacacaggc gcgttcacgg ctgagttttt cgcacacttt taaatagaag cagcgacgta
     9601 ctaaatccag acgggtgaaa tcttcaatcg ccgtcaggta ttcagtaaca cgctccagca
     9661 tcatgcagta tggatcgagg ccaaacgata caatctcgcc gtcgtgcaaa cgctgtttga
     9721 tatctttcgc cagcagacgt gggttcgggt attcccagga ataggcttcc agcagcagtg
     9781 ttttcagcac cgctttgtat ggggaatcga tactcttgta gagctgccaa aggctggcac
     9841 caaagtactc ttcagcagaa agcgagctta agccaccgag atccagccat tcatttggcg
     9901 tcagcacgcc ctgcgcgtaa agcgtcatca catagtcgtc gtaatgctct tcttcgtcgc
     9961 acggcaccat attccacaga atacgcttac cggcgagacg cacggcggta cgataaaatt
    10021 cgtcaagcag cagtatatgc tgggtggagc cacaatcttc gccccccagg ctgccgcttt
    10081 cattatgacg aaagcggttt tcatcaatca ggaagaagct gacttccaca cccagcgagg
    10141 cggcccagct ttccagcagg ctgcatttac gttgtagtaa ctggcgctct tcgctatcga
    10201 gccaggattg atgacagacc cagatatcca ggtcagagga acaactttgc cctacggacg
    10261 aggtgctgcc catggtgtat acaccagtaa ttggaagctc acctttcggt ggatcctgta
    10321 ctgacattcc acgatacagt tcaagctcgt tcaggtagtg gcgttgagtt tcatcaggcg
    10381 tgtaaaggca aatgcctttg ggaacgttac catcaaggta acccggcatt agcggatggt
    10441 gatagtgcaa caatgtcggc agtagactgt agacctgttg gaatgcaggc cccatagcag
    10501 caagcgcgcg atccacacgc aattgattta tggcatccag tctctgtttc agagtctcaa
    10561 tatagaggta caagacgtat cgcctgattt gctacccgtc atgactgtga ttccgccaac
    10621 atcaacggta acacgcggca ttcgggatat ttcgtatgtc aaaggtaacc gttaccactt
    10681 ttcgcgcctg gtttttttag tttcacgacg aaaaaatggt ctaaaacgtg atcaatttaa
    10741 caccttgctg attgaccgta aagaaagatg cgctacatac aagtgtagca ccgtttattc
    10801 tctgtaaatt ccttattacg acagcgtgaa acgtctgtca ggatccactg ccagacctca
    10861 ttttacggtt tgcgcaggcg tctacgtttc accacaacac tgacatcact ctggcaagga
    10921 tgttaggatg gaccacggat gataatgacg gtaacaagca tgttagacaa tgttttaaga
    10981 attgccacac gccaaagccc acttgcactc tggcaggcac actatgtcaa agacaagttg
    11041 atggcgagcc atccgggcct ggtcgttgaa ctggtaccga tggtgacgcg cggcgatgtg
    11101 attcttgata cgccgctggc gaaagtaggc ggaaaaggct tatttgtaaa agagctggaa
    11161 gtcgcgctcc tcgaaaatcg cgccgatatc gccgtacatt caatgaaaga tgtgccggtt
    11221 gaattcccgc aaggtctggg actggtcact atttgtgagc gtgaagatcc tcgcgatgcc
    11281 tttgtgtcca ataactatga cagtctggat gcgttaccag caggcagtat cgtcgggacg
    11341 tccagtttac gtcgccagtg ccaactggct gaacgccgcc cggatctgat tatccgctcc
    11401 ctgcgcggca acgtcggcac tcgcctgagc aaactagata acggcgaata cgatgccatc
    11461 attcttgccg tagccggact aaaacgttta ggtctggagt cccgcattcg cgccgcgtta
    11521 ccacccgaga tttctcttcc ggcggtagga caaggtgcag tcggtattga atgccgcctt
    11581 gatgatacgc gcactcgcga gctgcttgcc gcgctgaatc accacgaaac tgcactgcgc
    11641 gttaccgcag aacgcgccat gaatacccgt ctcgaaggcg gatgtcaggt gccaattggt
    11701 agctacgccg agcttattga tggcgaaatc tggctgcgtg cgctggtcgg cgcgccggac
    11761 ggttcgcaga ttattcgcgg tgaacgccgc ggtgcgccgc aaaatgccaa acaaatgggg
    11821 atttcgctgg cagaagagct actgaataac ggcgcgcgcg agatcctcgc tgaagtctat
    11881 aacggagacg ccccggcatg agtatccttg tcacccgccc gtctcccgct ggagaagagt
    11941 tagtgagccg tctgcgcaca ctggggcagg tggcctggca ttttccgctg attgagtttt
    12001 ctccgggtca acaattaccg caacttgctg atcaactggc agcgctgggg gagagcgatc
    12061 tgttgtttgc cctctcgcaa cacgcggttg cttttgccca atcacagcta catcagcaag
    12121 atcgtaaatg gccccgacta cctaattatt tcgccattgg acgcaccacc gcactggcac
    12181 tacataccgt aagcggacag aagattctct acccgcagga tcgggaaatc agcgaagtct
    12241 tgctacaatt acctgaatta caaaatattg cgggcaaacg tgcgctgata ttacgtggca
    12301 atggcggtcg tgagctaatt ggggataccc tgacggcgcg cggtgctgag gtcacttttt
    12361 gtgaatgtta tcaacgatgc gcaatccatt acgatggtgc agaagaagcg atgcgctggc
    12421 aatcccgcga ggtgacgacg gtcgttgtta ccagcggtga aatgttgcag caactctggt
    12481 cgctgatccc acaatggtat cgtgagcact ggttactaca ctgtcgacta ttggtcgtca
    12541 gtgagcgttt ggcgaaactc gcccgggaac tgggctggca agacattaag gtcgccgata
    12601 acgctgccaa cgatgcgctt ttacgggcat tacaataact ctcataacag gaagccataa
    12661 tgacggaaca agaaaaaacc tccgccgtgg ttgaagagac tagggaggcc gtggacacca
    12721 cgtcacaacc tgtcgcaaca gaaaaaaaga gtaagaacaa taccgcattg attctcagcg
    12781 cggcggctat cgctattgct ctggcggcgg gcgtcggttt gtatggctgg ggtaaacaac
    12841 aggccgtcaa tcagaccgcc accagcgatg ccctggctaa ccaactgacg gcattgcaaa
    12901 aagcccagga gagccaaaaa gccgaactgg aaggcattat taagcaacag gctacgcagc
    12961 ttgaacaggc gaatcgtcag caagaaacgc tggcaaaaca gctggatgaa gtccagcaaa
    13021 aggtcgccac catttccggc agcgatgcta aaacctggct gctggcccag gccgattttc
    13081 tggtgaaact cgccggacgg aagctgtgga gcgatcagga cgtcacgacc gctgcagcgt
    13141 tgctgaaaag tgcagacgcc agcctggcgg atatgaatga cccgagtctg attaccgttc
    13201 gtcgggcaat taccgatgat atcgccagcc tttctgcagt atcgcaggtg gattatgacg
    13261 gcatcatcct taagcttaat cagctttcaa atcaggtaga taacctgcgt ctggccgata
    13321 atgacagcga tggttcgccg atggattcag acggtgaaga gctttccagt tccatcagcg
    13381 aatggcgtat caatctgcaa aaaagctggc agaactttat ggacaacttc attacgattc
    13441 gccgtcgtga tgacaccgcc gtaccgctgt tagcgccaaa tcaggatatc tatctgcgcg
    13501 aaaatattcg ctctcgcctg ctggtcgcag cacaagctgt accgcgtcat caggaagaga
    13561 cttatcgcca ggcgctggag aacgtctcca cctgggtacg tgcttactac gatactgatg
    13621 atgccaccac caaagcgttc ctcgacgagg tggaccagtt aagccagcaa aatatctcga
    13681 tggatcttcc ggaaaccctg caaagccagg cgatgctgga aaaactgatg cagacccgcg
    13741 tgcgtaacct gctggcacaa ccggcagcgg ggacaacgga agctaaacct gcacctgcac
    13801 ctgcacctgc acctgcacct gcacctgcac ctgcacctgc accgcaaggt gatactccgg
    13861 cagccgcgcc gcaaggagaa taaccatgct aaaagtgtta ttactctttg ttttgctgat
    13921 tgcgggggta gtggttggcc cgatgattgc cggtcatcag ggttacgtgc tgatccagac
    13981 tgacaactac aatatcgaaa ccagcgtcac gggcctggcg attattttga ttctggcgat
    14041 ggtagtgctg tttgccattg agtggctact gcggcggatc ttccgcacag gcgcacacac
    14101 ccgtgggtgg tttgtcggac gtaagcgtcg ccgtgcccgt aagcagaccg aacaggcgct
    14161 gctgaaactg gcggaaggcg attatcagca agttgaaaag ctgatggcga aaaatgccga
    14221 tcacgcggaa caaccggtgg tgaactatct actggctgcc gaagccgcgc aacaacgtgg
    14281 tgatgaagca cgcgccaacc aacatctgga acgcgcagca gagctggccg gcaatgacac
    14341 cattccggta gaaatcaccc gtgtacgtct gcaactggcc cgtaatgaaa accatgctgc
    14401 acgccacggc gtggataagc tgctggaagt tacgccacgc catccggaag tattacgtct
    14461 ggcggaacag gcgtatatcc gcacaggtgc atggagttct ctgctggata tcatcccatc
    14521 aatggcgaaa gcccatgttg gtgatgaaga acatcgtgca atgctggaac aacaggcatg
    14581 gattggcctg atggatcagg cgcgtgccga taacggtagt gaaggtttgc gtaactggtg
    14641 gaaaaaccaa agccggaaaa cgcgtcatca ggtagcgttg caggtggcaa tggcggaaca
    14701 tcttattgaa tgtgacgatc atgatactgc ccagcaaatt atcatcgatg gcctgaaacg
    14761 ccagtacgac gatcgcctac tgctgccgat tcctcgactg aaaaccaaca atccggaaca
    14821 gctggaaaaa gtgctgcgcc agcagatcaa aaacgtcggc gatcgcccgc tgttgtggag
    14881 cacactgggc cagtcactga tgaagcacgg agaatggcag gaagcatcgc tcgccttccg
    14941 cgcagcgctg aaacaacgtc cggacgccta cgattacgca tggcttgccg acgcgctgga
    15001 cagactgcac aagccggaag aagctgcagc gatgcgtcgc gacggtttga tgttaacgtt
    15061 gcagaacaac ccaccacagt agttccttct cacccggagg caagcacctc cgggaccttt
    15121 ctgatgcata aaaaaacgcc tgctcttatt acggagcagg cgttaaaaca ggtctgtatg
    15181 acaacagtgg gtgcttcact caacgttgtg tccatggtgt ctgatgaggc ataagcgaca
    15241 tctgtcagtg gacgataagc accgtaaacg gctctgcgtc attccggagt ttatgaggca
    15301 ctaaggcgaa cataagagat ggaatgagca tctacccgtt tattatgcca cagagaatcg
    15361 ggaaataaca tcccttaaca cttgttatga gataattctg taatcctctt tgcttcctga
    15421 gtgaatattt aacctgagct tgatcctaca catacttatt atgaatgata aaattcattc
    15481 aattaataac acatatatta attgccgtta aaactaaaaa agcattaata atcaacgcaa
    15541 tataataaac ccgccttaca tatcaactgc gccagaggta ggattgaaaa cgctctcctg
    15601 attttccaat tcattttctg gataaataaa taatttattt ttgtcactat tatttatgta
    15661 atcatcctgt cagggagagg gatctcaatt atcaatgctt aattacgtca tcattttgat
    15721 gacatgaaac cggagcattt tgcatggaat tttcgttttc acccaaactt cttgttgttg
    15781 ctgtcgccgc cgctcttcct ctcatggcca gcgcagcaga taccccgtca actgccaccg
    15841 cacgcaaagg ctttgccgga tacgatcacc caaaccagta tccacagaac gctgcaattt
    15901 attgaatttg cacgatcatg taggccggat aaggcgttta cgccgcatcc ggcaatcaac
    15961 cgcaggcggc cacaaaatgg ccgctttttt acttattcac caccagcaaa tgtgcctgca
    16021 taatgtcact ggccgggcga ccgtgcgcca gcaaatcagc cattgcttta agatatggcg
    16081 gtagatggcg gaaataacgc tgatacccgg cacacaaata attcagcccc ggtttgccgc
    16141 tggcatcgag catgaagcgg tgtttcgggc agcctcccca gcacgctttt aacacgttac
    16201 aactgcgaca ctgcgccggt aattgcttaa atttatcttc accaaacacc tgctgttgcg
    16261 gggaatcgat catttctgca attgtttgct gatgcatatt ccccagccga tattgcggat
    16321 aaacatagtg atcgcaggcg taaacgtcgc cgttgtgctc aacaatcacc gagcgcccac
    16381 aggttggctg atgatggcaa accgcacccg gcgcaccgac aaaattggca aacgcccatt
    16441 cgatattcat cacgaaaatc ttgccgacgt cgcgtttgat ccagtggtcg aatatcgcca
    16501 ccagaaattc accaaattca tcggggcgca ccgaccattc cgtcagttcc ccctgaatat
    16561 caccaggcgc atgcaatttc agtccatcgc gggcagctgt ttcatcagcc agacgctcga
    16621 ccaccggaat aaactggatg aattcgactc ccgcatcgca caaaaaatca taaacctgca
    16681 acggttgctg cgcgctggtg cggttgacgc agaccagcac gttatagtcg acatgatgtt
    16741 tttgcagcag cgtcagggca cgcatcacca gcttatgcgt tggtctgcca cctttggtca
    16801 cgcgatattg attgtggatc tcagccggac cgtccagcga taacccaaca agaaaatgat
    16861 tttctgccag aaatgcacac catttatcat cgagcagcac gccgttagtc tggaagctgt
    16921 tacttatctt cctgccagca ccgtatttcg cctgtaactt tacggcacgg cggtaaaaat
    16981 ccagcccgag taacgttggt tcgccgccct gccaggtaaa agccacttcg ttttgcggtt
    17041 cgctggcagc gatatagtga cggacatacg cttccagcgt gtcatcgtcc atatgcgtga
    17101 ctggcttttc gcggtaaagg gattgttttt cgagataaaa acagtagtca cagttcagat
    17161 tacaatcgga accactcggt ttcgccatca catgaaaagc acgcgttgga acctgttgca
    17221 gcatggtcac tccttgcctg aagtcatcct tcaatacgtt aagggcgaaa tcataccgtg
    17281 gggcaatgag ttattacggg cgtttattgc gaagggaggg tgacgaaatg cacagaaaac
    17341 aaaaaacccc gccgaagcgg ggttcaaaat tggtcggcga gagaggattc gaacctccga
    17401 cccactggtc ccaaaccagt tgcgctacca agctgcgcta ctcgccgaaa tactgctttt
    17461 tgaattttta gttcaattct ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg
    17521 tccgtaagga cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcattg
    17581 tcacaacttc taataatggg gtggctaatg ggattcgaac ccacgacaac tggaatcaca
    17641 atccagggct ctaccaactg agctatagcc accactacaa atcttgttac gcggtattac
    17701 taccaccgca gctcaagcgc cgggactaaa tggcgcgccc gacaggattc gaacctgaga
    17761 cctctgcctc cggagggcag cgctctatcc agctgagcta cgggcgctta gcgccgttgc
    17821 ggggggataa tacggacttc ccacccctct gtctagtccc tttttaaata aattgcgcgt
    17881 ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt ttatgcgctt tcccgtggcg
    17941 attaaggcca aaaactttat aaatcgccgt caccgccagc ataaagatga tgccaacaaa
    18001 cagcgacata cgcgtatctt cattaaagta catgccgatc aaaacgcaaa tcaggaatgc
    18061 cattgttacg tagttggccc acgggaacag gattgagcgg aacggatggc tggcaatcgc
    18121 cgctttatgt gcacgccgaa aacgcagctg gcttatcaat atcacaaacc atggcaccat
    18181 ccccggaagc acgctggcac tgtagacgta gacaaacaca cgctgcggat tgggaatgat
    18241 gtagttcagg catgagccaa ttagcagaat agcaatagat actgccacac ccgcaactgg
    18301 tacgccgtga cgggaaactt tcgccatcgc cgccggtaac tgacggtttt tcgccagtgc
    18361 gtagagcata cgtccgcaac tgtacatgcc gctgttacag ccagagagcg cagccgtcag
    18421 caccacaaag ttgataatgc ccgccgctgc ggtaataccg attttggcaa aagtcagtac
    18481 gaacgggctg ccgttgctgc caatttcatt ccacgggaag atggtgacga taacgaaaat
    18541 cgcgcctacg tagaaaatca ggatccgcca cagcactttg cctacagcac tgcgcagcgt
    18601 cacctgcgga ttcttcgctt caccggcagt aatgccaatc agctccacgc cctggtagga
    18661 cgccaccaca atacacagcg cggtcaggaa ccctttccag ccacccgcaa agaaaccgcc
    18721 atgctctgtg agattgctaa aaccaatcga ctgcccgcca ttgccaaagc caaagaaaat
    18781 cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg actttgatca tcgcgaacca
    18841 gaactcgatt tcgccgtaca accgcaccgc cgccagattc gccaacgcca ccagcgccac
    18901 cgcgatcaat gcaggtatcc actgcgccat ctccgggaac cagaactgga cataaacgcc
    18961 aatggcggtg atttctgaga tccccaccgc catccacata aaccagtaag accaggcggt
    19021 gagataacca aaaaacgggc tcatataacg atgcgcataa acggcgaacg aaccggtaac
    19081 aggttcaagg aacaacattt cgcccattga acgcatgatg aaaaagacga acagcccggc
    19141 gatgatatag gccaacaata cggatggccc ggcccatttc agggtactgg cggcccccat
    19201 aaacaggccg acgccaatag tgccccccag ggcgatgagt tcgatatgtc gagcttccag
    19261 cccacgctgt agctctggtt tgttatctgc cataaatcct cgtgttgtgt ttgcatgctt
    19321 tccggtgtta ccggttatcg ttatgggtac atcgagtgtt gcgaatgttt tcgtaattca
    19381 ggagaaatgg caaataaagc attaaaaatt tgaatgcttt gtgtaataaa aaagcagaca
    19441 ggcgacggag tgaccactcc gtcgctttac aaagagagga aaatcagagg ttgccggtgt
    19501 agtgccagcg taaataacgc agcaaacgaa gctgacgctt aatgcggctc ggctgcgaaa
    19561 gcaggcggta gagccactcc agtcccagcg tttgccagat tttcggtgcg cgttttacgt
    19621 gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata cagcgcatct ggatgcacca
    19681 gacggcagtc gcgcatgaag atctcctgct ttggcgatcc catcgcaacg gtgacgattt
    19741 gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg ctgctcgggt ttaaaataac
    19801 catcctgact gccaacgata ttcacattcc attggttgcg cagtttagct tcagtttgcg
    19861 ccagcacttc aggtttaccg cccacaagaa ataccggcgt cccttctttg cctgcgcgcg
    19921 ccatcagctc ttcccagaga tcggcaccgg caacgcggga aacctgcgcc tgcgggtact
    19981 ttttacgtac tgaacgtaca acgctgatgc catccgcata tttaaattcg gcagcgttaa
    20041 ttaactccct gacctcggcg ttatcttcaa tggtcagcat tttttcagca ttaatggcaa
    20101 ccagcgttcc ctgcttaagc tgcccgtcag caaacagata atcgagggcg tgctgcatat
    20161 cacgccaacc aatcaactgt aagccacgca gcgtataggt cggtgccgtg gtgttgttat
    20221 tcattgttat ccttcaacct gcgtccggag cgatgatttt gtacgtttat gaatgagtcc
    20281 ggcgctttca aaaagccagt acagcagttt tgcgatcatc agacatgcgc cgaagaccac
    20341 gataaaaaag actacgcgtg agacaaacga atccagccct tcacgcgcca gcacgatcat
    20401 attgaagatc gccccaaagc agaaactgtg caatatcgca gccttatagc gattaggctc
    20461 gcggttgccc agctcataca gccagtcgaa ccatttgatg atcagaccaa ccacgatcgc
    20521 cccgagcggg atgaacaacg cgccgcccat caccaccagt gagcctataa gcgtaggcga
    20581 gatcgccagt ccggagtggt tattcagcac ttcccaggta aagtagttgg ctgagttcag
    20641 caccatactc gggcgacccg gccacagcca ggaagggata aagacataga aatcgcggac
    20701 aattggagcc aggccctgga agtcgatgtt gtcgtagttc tgcaacagca acgccagatt
    20761 ctcccacggg gagaaggtgt cgcgagtgag atagagaaac gtatagaacg cttcatcgcc
    20821 gctcacattc attccatagc gttttagcgc cagccagaac atgccaacaa tccccagcac
    20881 gcccgccgcc gccagcatcc acaacgaaat ccagccgcga ataatgccaa taaacaggaa
    20941 gatagcgaat gcgatgatga tattggcgcg agtgccgccg acaatcatat aagtcagcaa
    21001 gccaaaggcg accgtgctga cgaggaaaaa cagccacgct ttgctgtcct ggcgcagaaa
    21061 gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag cgttttaacg ccacgccgga
    21121 gacttcactg gagaagatct gactgtagga gttaagccgg aacagcaaaa agccgttgtg
    21181 catgaagaag atgccgacgc ttaccagcgc gatacccatc aggatcaccc acgtaagatt
    21241 ggtctccacg cggttcatgg taaacagcgg acggcgcggt acatcagcaa cgcgtttgcg
    21301 taggcgggtt ttgtaggtga catagtaaac cgcgtagaag cagcccgcag aaagcaacgc
    21361 ctgcaacaag atttctggcg gcgcgacacc aacatcaaag cgaaatacca gcacgctggt
    21421 cagcgggaag ccgaagaaaa aggtgagcaa aaacagcaat gaaaagaaga cattgaagtt
    21481 aaagcgcaca cggcgaaact caaaccaggt cagcgtggca ataaacagcg tgcagagcag
    21541 ccagaccaca aacaggccac tgaattgcag cagactcatg cgacctcccc ggcggcaata
    21601 gccaacgccc gctgccagcc ttgtagatag ttagggctaa agaaggcaat ggtgttttta
    21661 tccaccgacg ccaactggcg ctgcgcttca cgcacaatat cctcgttgag atcgtcggta
    21721 gtaaacagca ccggcaaatg ctgttccgtc atatcctgcc agaacggatt ttcccggtta
    21781 agcacacaag gaatgcccgc ctgaatcagt aagcaaagcg taccaatgcc ctgctggcgg
    21841 gcaaaaataa agtaaccaag atcgcactga cgaagtagcg ccagataggc gtcaaattcc
    21901 agtttttcgc tcaaaatttg cagattttct tcgctgaata actccagccc cgcctgacga
    21961 acttcctcaa tgtacgcttc gttattaggc ggatatccca tcggcaccac cacttttacc
    22021 gtatcgccaa attgctgatg aacggcgcgc aaggcagcaa tatggtcatt gctgcggtca
    22081 ccggagttcc ccaccagaat ggtcattttc ccttcacgtt gccgatcatt cgccatcgta
    22141 ttgagagaag ggtccatccg cgtcgggaag tacagcagtt cgccccgcac ctttggatgc
    22201 gttttggcaa aaaagctcaa atcaccgcgg gtggcaaata cacagccgac tcgcttttgc
    22261 gccaggcgac gtagcgggta aaaaagctta tatctcaagc cactggaaag ctcatacagg
    22321 tctgcccccc agatatgcca gaaaaactgg ctgggcttaa tgccaccact cagcagagcc
    22381 agccacagtg tgggattgaa ctgaccgtgg aaaaaaaatc gctgctgacg gttagctttt
    22441 gctttcgcga tgaccgcttc cgccagcgat tttttcccag ggaaaaattg cacagaaagc
    22501 gccggacagc tatcacttaa gccgtcgtcc ttgccaacaa ccataaactc gcgcgcgtgc
    22561 tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa cggttcggtt atggtgaggg
    22621 atatccgatc ccagtacgtg aatcagtaca gtcatgcccg cctacgccag agtaaaaaca
    22681 cgccacaaca aagagaaaaa tagacgatat aagttgccat atatgcctgc gccgcgccca
    22741 gtgcaccatg cgcagggatt agccagtggg caaataccat caataaagtg aactggctga
    22801 cttccgccag aatataaaac cgcagtgacg ctttggcgat caccagataa ccaaagacat
    22861 aagcgcccac ttttaacaca tcacccacta actgccaggc aaagagatcg cgcatagcgg
    22921 taaatttatt cgacaacagc aaccagatag caaaatcacg cagcaaccag acggtaaaac
    22981 tcgccgccgc cactgccggt aagacgaatt tcagcgattt aaccacttcc cgggtgatat
    23041 cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata tacgctgaac gatgccgtaa
    23101 taaattgcag gtaggcatcg gaaatactgc tcaccccttg ccagatcccc acttcatccc
    23161 agctatactg cgccgccagc agtttacgca tcatgatgta agcaacaggc aaggtcaccg
    23221 acgtaatcaa cgccatgagc gtaaatttgc tcaactgccc tgccagaccg ttatcccagc
    23281 tgggtttcag atagcttaac gggatgacac cacgtttgat caacatgatg gcggcaggaa
    23341 tcaccaccag cgcgggaatc agcgccagac ccagcaacgc cccttcataa ccgcccaaac
    23401 ggtaactgac gtagtacgcg agaacgccaa tcaagctgcc gacaatcagc gataacgcat
    23461 tacctgcggc atcgcgaaag cctttcatca gcgccagtaa caggttgccc caggcgatcc
    23521 ccatttgcac cagcgccact aaacgcacca gcccctgata gtcggtatta ccaaacagtc
    23581 cctggctgat tggcgcagct gccagcacaa aaaccagcgc catcagcgta gagaaaccaa
    23641 gtaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg ttgcggatta tcatggtact
    23701 gggcaacgta tttggttaca ccgttaaaga tgccagctcc ggcaagcacg ccgagcacgg
    23761 taatcaactg gcggaaattt gccgccagcc caagccccgc cggaccaaat gacacagcca
    23821 gcaacttacc gaccagtaac ccggcaccaa tcttgaccag tgtactggcc gccgtccaca
    23881 aggacgcttt tgccaacgac atatcaggag aagtagttca acaaagtcgc aattaccgta
    23941 cgctgattga cgggcgacag gttgtagaac agcggcaggc gcagcaggcg ctcgctctct
    24001 ttggtggtgt agcgatcttc accgtggaac tcaccaaagc gttcccccgc agggcaaccg
    24061 tgcagcggaa tgtaatggaa caccgccatg atttccgctt ctttcagaaa gttaatcaac
    24121 gcgctccggt catcaatatc ccgcagttta atgtagaaca tatgcgcgtt ctgcacgcag
    24181 ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg ccagaggcgc taacgcatcg
    24241 tagtagtttt gccacagcgc cagacgttgc tggttgatac gatccgctgc ttccagttgc
    24301 gcccacaggt acgcagcttg cagatcggac atcaaatagc tggagccaat atcgcgccag
    24361 gtatatttat cgacctgacc acggaagaac tggctgcggt tagtgccctt ttcacggatg
    24421 atctcggctc gttcgattaa cgctttatcg ttaatcagcg tcgcgccgcc ttcaccaccc
    24481 gccgtatagt ttttggtttc atggaagcta aagcagccaa tatgaccaat ggttcccagt
    24541 gcacgccctt tgtaagtgga catcacgccc tgagcggcat cttctaccac aaacagatta
    24601 tgctttttcg ccaacgccat aatggtgtcc atttcgcagg ccacacccgc gtaatggacc
    24661 ggcacgataa cgcgcgtttt gtcggtgatc gccgcttcaa tcagcgtttc gtcgatgttc
    24721 atggtgtccg ggcgaacatc cacaaaaacg atttttgcgc cacgcagcac aaaggcattg
    24781 gcggtggaga caaaggtgta gctcggcatg atcacttcat cgccaggctg gatatcgagc
    24841 agcagcgccg ccatctccag cgaagcggtg caggacggcg tcagtaacac tttggcgctg
    24901 ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa aaccgccatc gccacacagt
    24961 ttgccgctac ccattgccga ctgcatatag tcgagttcgg ttcccaccac cggcggtgcg
    25021 ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg gtgctttcta cattcgcacc
    25081 actttgtatg tatcgtttaa gcgcggcggt gttgcccatt tgggtcgcca cccgcaaagt
    25141 tgttttaccg cgagcatacg cccagtttag cgccgtttgc atcagctcag cacctgcacc
    25201 gcgtccagcc agcaggccaa ttcgcgcatc tgtcgcattg agttcccgta aagagacata
    25261 accgcgaata ccgccggacg ccgcacgtaa aatcagacat tgatgatcaa aggtgccgcg
    25321 cacggcattt tcaatccact gtgcataaaa gcgtccgctg gcgtcaggcg cataccacgg
    25381 cgcacgaaaa cggctttgcg caaatgcggc gctggctaac tgacgtaatg cgggaatatc
    25441 ggtctcttgt gccactacag caccactatc actgacattg ttcacgggta gtgccaaatc
    25501 aacttcacct tctaccaggg agaatcccag ctgttgcagg gcatccagtt cacccgtatt
    25561 tgatgccgca attttggcct gcacccgtga ccacggcgct aacacgtctg gcgtcaggag
    25621 ctgtgcttca gacgtaatgc gcacgatggc gctgttaaca ccaaagaagg cgttttccca
    25681 ggttagtggc tcaatactgg cgcggacggg cacgaagtaa ctccagcaga tattggccgt
    25741 agccagtttt cgctaatgaa ctggcagcac gcttcacacc ctcgtcatcg agccagccgt
    25801 tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc ctggcgtttt tctactgtct
    25861 gcacaaaggt actggcttca atcaggctgt cgtgagtgcc agtgtccagc caggcaaatc
    25921 cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag gtacatctgg ttgatggagg
    25981 taatctccag ttcaccacgc tccgacggct tcacctgctt tgcgtactcc acgactttac
    26041 tgtcgtagaa ataaagcccg gtcaccgccc agtttgactt cggctgtttt ggcttttctt
    26101 ccagcgagat agcgcggaaa ttgtcgtcaa actccaccac gccaaagcgt tccgggtcca
    26161 tcacctgata gccaaatacc gtcgcccctt ccgtgcgcgc cgcaacatga cgcagcttcg
    26221 gactgaagcc ctgaccgaag aagatgttat cgcccagcac cagacaagaa ggttcaccgt
    26281 tgaggaaggt ttcaccgatg ataaaggcct gcgccagacc gtccgggctg ggctgttcgg
    26341 cgtattccag ctggataccg aactcactac catcgcccag caggcgctgg aaataacctt
    26401 tatcttccgg cgtagtgatg atgagaattt cgcggatatc ggccaacatc agcaccgaca
    26461 gcggatagta aatcattggc ttatcgtaaa tcggcaacag ttgcttcgat acgccgcgcg
    26521 taatcggatg caatcgggtg ccggaaccgc ccgccaggat aatacctttc atgtttgcct
    26581 ccgctgaaaa ttagcctttc agacctaaac gctcgccctg atagctgccg tcctgcacct
    26641 gcttccacca gctttcatta gccagatacc actgcaccgt tttacgcatt ccactttcaa
    26701 aggtttcctg tggcagccag ccaagttcac gggcaatttt cgaagcatca atggcataac
    26761 gcagatcatg ccccggacgg tcagcgacaa aggtgatcaa gtcacgatag tgcaccacgc
    26821 cgtgcggctt gttcggagcc agttcttcca gcagctcgca aatggtttcc acaacatcga
    26881 gattcttacg ctcgttgtga ccaccaatat tataggtttc accgactttc ccggtggtcg
    26941 ccacgcaata cagcgcgcgg gcgtgatctt ccacatacag ccagtcacgg atttgctgcc
    27001 cgttgccata taccggcagc ggtttacccg ccagcgcgtt gaggatcatc agcgggatca
    27061 gtttttccgg aaagtggtaa ggaccgtagt tattcgagca gttggtgata agcgtcggca
    27121 gaccgtaggt ccgcagccag gcgcgcacca gatggtcgct gctggctttt gacgcggaat
    27181 aagggctgct cggcgcatac ggcgtggttt cggtgaagaa atcatccgtc gagtgcaggt
    27241 caccatatac ttcgtcggtg gagatatgat gaaaacggaa cgctgatttt ttatcttccg
    27301 tcagcgtatt ccagtaagcc cgcgccgctt caagcaatgt ataagtcccg acaatgttgg
    27361 tttcaataaa cgctgccggg ccgtcaatag aacggtcaac atggctttcg gctgccagat
    27421 gcatgacaca gtctggctga tgctcagtga atacgcgtgc cagttctgcc cgatcgcaga
    27481 tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac cggtgccagc gacatcaggt
    27541 ttccggcgta ggtcagctta tcgaccacta ccaccgcgtc gctcgtttcg ttgatgatat
    27601 aacgcaccag cgccgagcca ataaacccgg caccacctgt tatcagaatt tttctcatca
    27661 gcgccagact cctttggcat cgacgacata ctgctgatgg acattgtcgc cattgataac
    27721 tttaaactga ctatgatcga ccagcatcac cagcacgtct gccgttgcca gcgcctcgtc
    27781 aagctgcgcc agagtacaaa gcccggtcag ttttttcggc aactggtgga tgttaggctc
    27841 aacaaccaga gtttcgccgc tatgccactg ggcgatcagt tcagcgattt ccatcgccgg
    27901 gctttcgcgc aggtcatcaa tattcggttt aaacgccaga ccaaagcagg cgattttcag
    27961 ttcactggcg cgtttatcgg tagccgccag gcaatcagcc accgccgctt tcacctgatc
    28021 gataacccag aacggtttgt gatcgttcac ttcgcgcgcg gtacggataa gccgcgcctg
    28081 ctgggggttc tgtgccacga taaaccacgg atcaacagca atgcagtgac cgcccacgcc
    28141 agggccaggc tgaagaatat taacgcgagg gtgacgattc gccaggcgaa tcagttccca
    28201 gacgttaatc ccctgatcgg cacaaatcag cgacaattca ttagcaaaag cgatattcac
    28261 atcgcggaag ctgttttcgg tgagcttaca catttccgcc gtccgcgagt tagtgacgac
    28321 acactcacct tcgaggaaaa ttttgtacag ttcgctggcg cgggtcgaac aaaccggcgt
    28381 cataccacca atcacgcgat cgtttttaat cagctcgacc ataacctggc ccggtaacac
    28441 gcgttccggg cagtaagcaa tgttgacgtc cgcctgctcg cccacctgct gtgggaaagt
    28501 gagatccgga cgcatctctg ctaaccattc tgccatcttc tcggttgacc ccaccggcga
    28561 ggtggattca aggatcacca gcgcgccttt tttcagcact ggcgcaatgg agcgagcagc
    28621 cgattcaacg taggtcatat ctggctcatg atcgccttta aacggcgtgg gtacagcaat
    28681 cagccaggca tccgcttcaa ctggcgtcgt gctcgctcgt aaaaaaccgc cttctacggc
    28741 agtttttact acgctcgcca aatcaggctc gacgatatgg atttcgccac gattgatggt
    28801 atcaaccgcg tgttggttga tatcgacacc aatcacctgt ttttgccgtg aggcaaacgc
    28861 tgctgctgta ggcagcccga tgtaacccag tccgataaca gaaatggtcg caaaactcat
    28921 agtgatatcc gattattttt taacgcttcc agaatgcgag agcatgcctg accatcacca
    28981 tacgggttat gggcgcggct catagtttga tattcgtttt cgtcttttaa aagacgcgtc
    29041 acttcctcga caattcgctg cttatccgtg cctaccagac gcaccgtacc cgccgtcacc
    29101 gcttccggac gctcagtggt atcgcgcatc accagcaccg gtttccccag cgaaggcgct
    29161 tcttcctgaa tgccgcctga gtcggtcaaa atcagccagg cgtggttcat cagccagaca
    29221 aacggtaaat actcctgggg atcgatcaga atgacatttt tcacatgccc cagaatgcga
    29281 ttgaccggct ctctgacgtt cgggttgaga tgcaccggat agacaatctg gatgtcctgg
    29341 tgcgtggtgg caatgtctgc caacgcgtgg cagatttctt caaagccacg accgaaactt
    29401 tcacgcctgt gaccggtcac cagaatcatc tttttatcgg ggtcgataaa cgggtaattt
    29461 gccgccagtt ctgaacgcag cgtatcgctg ctcatcacct ggtcacgcac ccataacagt
    29521 gcatcaataa ctgtattacc agtaatgaag attcggctat ccgcaacgtt ttcacgcagc
    29581 aagttttgcc gggaagtttc ggttggagag aagtgataca tcgccagatg cccggtcaat
    29641 gtacggttag cctcttccgg ccacggcgaa taaagatcgc ccgtgcgcag accagcctca
    29701 acgtgaccaa caggaatacg ctgataaaac gccgccaggc tggttgccag cgtcgtcgtt
    29761 gtgtcgccgt gaaccagcac gacgtctggt ttgaactcgg caagaatagg ttttagccct
    29821 tccagaatcc gacaggttat ctctgtcagg ccctgtcctg gctgcattat gttgagatcg
    29881 tagtcaggta caatggaaaa gagtttcagc acctgatcga gcatctcccg atgctgcgca
    29941 gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca acgcatgcac caacggcgcc
    30001 atcttgatgg cttccgggcg cgtaccaaat acagtcagta ctttcacatc gattctcttc
    30061 gaataagcgg cgagcgcctt tgcgctcacc gcagcagtgt tgctatttcg agcaacggcg
    30121 ggttaatgcg acaccagccc cgatcagccc cccgacaatg ccccacataa tcatcaggaa
    30181 ggcacgacgt gggctatcgc gttttaccgg ttcttccggc gtacgcaaat agcgataggt
    30241 ctgaaaacgc ggatccaggg ttggaccaac attcagggtg tttaacatgg cccgattctg
    30301 atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa ttttccagtc gagcctggag
    30361 cattggacgc ccaagcagga acatttctga atcaggtaat tcctcggcag gcacatctgt
    30421 cgcactgcgc gaaatattat gctgctcagc aattttcagc gcctgttcaa tactgttcat
    30481 ccggcggtcg tagatggctt tcgccacctc ttcctgacgc ttcacctgtg ctttcatctg
    30541 gatggtacgc gccgcccatg cgcctttcag ctcatcattc agatggctgg ctgcacgctg
    30601 gctggcaaaa gcaacatact gacgtaacag gttattagcg tcaggcgcgg tttcagcaat
    30661 aagcttcacg ctgtcattga ccgcgcgggt aaagtctccg gggataaact ggatgttgtt
    30721 aatcatttca tccagcaacg ccgcatcggc tttgctgttg cccaccatcc gctgtttgta
    30781 atagtcggtt tgcagccaga actctctgcg ggtatcccac gaggccagtt gcataacaaa
    30841 ctctttatag gcttcgtcca tgaccgatgg ttggtcggca gaagccatgt ttgaacggac
    30901 atccaggtta cgcaaaaatt gctgctgcga gtaatatccc cccagcatat tcaccgttgg
    30961 acgatcggta atcgccgtcg agctccactc ctgacgagca aaaaaagtat acgccagcgc
    31021 gattaacgca aatgccagcc ccatgccaat aatccatagc ttcccagccc acaaggtacg
    31081 aaacaaccca cgaatatcca gttcattttc agcgtcttcg gccggtttcc caggcattgg
    31141 ttgtgtcatc acatcctcat ttatttggtt aaattggggc tgccaccacg atttctacgc
    31201 agtctgcgtt ttacgcgctt aataaagcga gcaactttcc aggcacgctt aatgcaatat
    31261 ccatagagga agaatgctag caaaaagagc accagcatga cccactccgg gacaaaatga
    31321 gaatattctg ccagcacgcc aatggaagcg agcagtgccg cagcaagggt aatcagcaca
    31381 aacgcctgac gggaagtaaa cccggcacgc atgatcaaat ggtgaatatg ctgacggtca
    31441 ggagagaatg ggctcatgcc tttacgcagg cgacggtaca taatcgccac catatccatt
    31501 agcggaatgg cgattatcca caaagcggta accgggctga tgggatgggt tttgccctgg
    31561 gtcgtttcga gcaggatcca gataacggta aaaccaatca gcgtactgcc cgcatcaccc
    31621 ataaagactt tgtagcggcg acccaggata ccaaggttaa gcatgatgta tggcaggatg
    31681 gcggcgatca tcgcaaagca ccagattgcg aggctggttt gcccgtcgaa ccacaaaatc
    31741 ataccgattg ctgcaaacga gacgcaggac aacccgccca gcaagccatc aatgccatca
    31801 accatgttga acgcattaat ggccgcccag acggcaaata gcgtcaggaa gtaaccaaac
    31861 ggtccgagca ccatctccca ggagccaaag atataaccca ggctactgag ataaagtttg
    31921 ccgaacacca tcataacaat gccaacagcg gcctgtatgg tggcacggat ttttacgctg
    31981 atatcaaaac ggtcatccag cgcgccaatg aaaacaagca caccggcaca agcgagatag
    32041 agagatgcat gcggaatata gtaatcgaca attccgaacg tgaagcaaat ccctgcgtaa
    32101 accgaaatcc ccccaacgag aggtatcaat ccctggtgac gtttgcggaa gtttggttta
    32161 tccactaaac cgactttttt tgccacctta cgggcaaaaa acagaaacag tgtcgtgaat
    32221 aaaaaaatac tgatgagatc agtactcact gtcagtaaat tcacaatgca tgctctcaga
    32281 gaaaattatt agcagaagta taaccacgaa gacctttatt cagaagggaa agtccgttcc
    32341 gaacccacca gtcctgtgca aaaattaacc atttgatatg caaattgcat aacacgccag
    32401 caaattccag gaattttcct aacatcgcca gcgcggcatt aagattacag atataaaagc
    32461 aaaacgccac gtaaacacgt ggcgtttttg gcataagaca aatttatgag cgtttcatca
    32521 tttcgaagaa atcgtcattg gtcttggtca tggccagttt attaatgagg aattccattg
    32581 catcgatttc gcccatcggg tgaatgattt tgcgcaggat ccacattttc tgcagttctt
    32641 cctgagtagt gagcagttct tctttacggg taccggaacg gttgtagtcg atagccggga
    32701 agacgcgttt ttcagcgatc ttacgagaga ggtgcagttc catgttgcct gtacctttaa
    32761 actcttcgta gataacttcg tccattttag aaccggtatc gataagcgcc gtcgcgataa
    32821 tagtcaggct gccgccctct tccacgttac gcgccgcacc aaagaagcgt ttcggacgat
    32881 gcagggcgtt ggcatccaca ccaccggtca acactttacc tgacgccgga acaacggtgt
    32941 tgtaagcgcg cgccagacga gtgatggagt cgagcagaat gataacgtct ttcttgtgct
    33001 caaccaggcg tttggccttc tcgatcacca tttccgcaac ctgaacgtgg cgagatgcgg
    33061 gttcgtcaaa ggtagaagca acaacttcac cttttaccag acgctgcatc tcggttactt
    33121 cttccggacg ttcgtcgatc agcagaacca tcagcacaca atccgggtgg ttgtaagcaa
    33181 tgctctgggc aatgttctgc agcagcatgg ttttaccggc tttcggcggt gccacaatca
    33241 gaccacgctg accacgaccg ataggtgatg ccagatccag tacgcgagca gttaaatctt
    33301 cagtagaacc gttaccacgt tccatacgca gacgagaatt tgcgtgcagc ggggttaagt
    33361 tctcaaagag gattttgttg cgggcgtttt caggtttgtc gaagttaact tcgttaactt
    33421 tcagcagcgc aaaatagcgt tcaccttctt tcggcgggcg aatcttacca gagatggtat
    33481 caccagtgcg gaggttgaaa cggcggattt ggctagggga aacgtagatg tcatcaggac
    33541 cggcgaggta ggagctgtct gcggaacgga ggaaaccaaa tccatcctgc aatatctcca
    33601 gtacgccatc accaaagata tcttcgccac tctttgcgtg ctgcttcagg atggcaaaaa
    33661 taatgtcctg cttacgcatg cgagccaggt tttccagccc catattttcg ccgagagtga
    33721 tcagctcaga aaccggcgta ttctttaatt cggtaagatt catagtggtg tgagttctta
    33781 aacttggggt aaatctcgaa cttaatgttg tgaatggtat ggcaggatca tccatgcctg
    33841 tttacggtca tcgactcatg tcttttcgct gcctggtcac aggagagtac gcagaactga
    33901 aacgacaaga cggattgagt gacgagccag aaatctgttc acttcgcatt taagataaaa
    33961 cgggaagcgt tggaaattac aagattcaaa cttaataagg tatgtttaat acgaagtcaa
    34021 cactaagtta gcatgactca cgccgggcgt ccagttttta gcgacggggc acccgaacat
    34081 gaaattccct tacgccaggt tagcgtcgag gaactctttc aactgacctt tagacagtgc
    34141 gcccactttg gttgccgcca cttcaccgtt tttgaacagc agcagagtcg ggataccacg
    34201 gatgccatat ttcggcgcag tgccagggtt ttgatcgatg ttcagttttg caacggtcag
    34261 tttgccctga tattcgtcag cgatttcatc cagaatcggg gcgatcattt tgcacggacc
    34321 gcaccactct gcccagaaat cgacgaggat cgccccgtcc gctttgagta catccgtgtc
    34381 aaaactgtcg tcagtcaggt gaataatttt atcgctcata tataactcca caggaataag
    34441 cctggcgtgt tggtttcgtt gttggtgtaa cattaaccaa ctaaaggttg actttatttc
    34501 accggatacg ctttcgtaaa gcaatagtaa gctgatattc taccacacta tgagcaaaac
    34561 acatttaaca gaacagaagt tttccgactt cgccctgcat ccgaaggttg tagaagccct
    34621 tgaaaaaaaa gggtttcata actgtacgcc cattcaggca ctggcccttc cgctgacgct
    34681 ggcgggtcgt gacgtagccg ggcaggcgca aaccggtacc gggaaaacga tggcgtttct
    34741 tacgtcaacg tttcattatc ttctctctca tcctgcgatt gccgatcgca aggtgaatca
    34801 accgcgtgcc ttaattatgg caccgacgcg tgaacttgcc gtgcagatcc atgccgacgc
    34861 agaaccgctg gcggaagcta ctggcctgaa gctgggtctg gcttacggtg gtgatggcta
    34921 cgacaaacag ctgaaagtgc tggaaagcgg cgttgacatt ctgattggca ccacgggtcg
    34981 tttaattgac tacgccaagc agaaccacat taacctcggt gccattcagg tagtggttct
    35041 ggatgaagcc gatcgcatgt acgatctggg ctttattaaa gatatccgct ggctgttccg
    35101 ccgtatgccg cctgcaaacc agcgcctcaa catgctgttc tccgccacgc tttcgtaccg
    35161 ggtacgtgaa ctggcgttcg agcagatgaa caatgccgaa tatattgaag tggaaccgga
    35221 acagaaaacg ggccaccgta ttaaagaaga gcttttctac ccttctaacg aagaaaaaat
    35281 gcgtttgctg caaacgctga tcgaagaaga gtggccagac agagcgatta ttttcgccaa
    35341 taccaaacac cgttgtgaag agatctgggg ccacctggcg gcagatggtc atcgtgtcgg
    35401 tttgttgaca ggcgatgtcg cgcagaaaaa acgtctgcgt attcttgatg aatttacccg
    35461 tggcgatctg gatattctgg ttgccaccga cgttgccgcg cgtggtttgc atattccggc
    35521 agtgacgcac gtctttaact acgatttacc cgatgactgt gaagattacg ttcaccgtat
    35581 tggtcgtaca ggtcgcgcag gcgcaagcgg tcactctatc agcctggcgt gtgaagagta
    35641 tgcattgaat ttgcctgcta ttgaaaccta tattggtcac tcaattccgg taagcaaata
    35701 caatccggac gcattgatga ccgatctgcc aaaaccgctg cgcctcacgc gcccacgtac
    35761 aggcaatggt ccgcgtcgta ctggcgctcc gcgtaatcgt cgtcgttcag gttaaataaa
    35821 atgtcatact caaaatcatt caaactgtat caagatggca agagaatgaa tcccgatgag
    35881 catactgtag taagtgattc aggtgaaaga atgcagccaa cacagagaca gattgaagga
    35941 tgaagagtat gggttccacc tcgtcgctgt atgcagccat tgatctcggt tcgaatagtt
    36001 ttcatatgct ggttgtgcgc gaggtggctg gaagcatcca gacgctgacg cgaataaaac
    36061 gcaaagtgcg tctggctgct ggcctgaata gcgaaaatgc cctgtctaat gaagcaatgg
    36121 agcgcggttg gcaatgtctg cgcctgtttg ctgaacgtct gcaagatatc cctccctcgc
    36181 aaattcgcgt tgtcgctacg gcgacattac gtcttgccgt caatgcgggt gattttattg
    36241 ccaatgcgca ggaaatcctc ggttgtccgg tacaggtgat cagcggtgaa gaggaagcac
    36301 gtctgattta tcagggcgtt gctcacacaa ctggcggtgc cgatcagcgc ctggtggtgg
    36361 atataggcgg tgccagtact gaactggtaa ccggcacggg tgcacaaacc acctcgttgt
    36421 tcagcctgtc gatgggctgc gtcacctggc tggaacgcta ttttgccgat cgtaatctgg
    36481 ggcaggaaaa ttttgatgct gcagaaaaag cggcacgcga agtgttacgt ccggttgccg
    36541 atgaattacg gtatcacggc tggaaagtgt gcgttggtgc ttccggcacc gtgcaggcgt
    36601 tacaggaaat catgatggca caggggatgg atgaacgcat taccctggaa aagttgcagc
    36661 aattgaaaca gcgagccatt cattgcggtc ggctggagga actggagatt gacgggctga
    36721 cgctggaacg tgcgttagtg ttcccgagtg gtctggcgat cctgatcgcc atttttaccg
    36781 aactgaatat tcagtgtatg accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg
    36841 gcatgttgca tctggccgtc gagcaggata ttcgcagccg tacgctgcgt aatattcagc
    36901 gccgctttat gatcgatatt gatcaggcgc agcgcgtagc caaagttgcg gctaacttct
    36961 tcgatcaggt ggaaaaggaa tggcatcttg aagcaataag ccgcgatttg ctcatcagcg
    37021 cctgtcagct tcatgaaatc ggcctgagcg ttgactttaa acaagcgccg caacacgctg
    37081 cttatctggt gcgtaatctg gatcttcccg gttttacccc cgcacagaaa aaattgctgg
    37141 cgacgctact gctcaaccag actaatccgg tcgatctctc atcgctgcat cagcaaaatg
    37201 ccgtaccgcc gcgcgtcgca gaacaactct gccgtttact ccgcctggcc atcatttttg
    37261 ccagccgtcg ccgtgacgat ctcgtgccag agatgacatt acaggctaac catgaactgt
    37321 tgaccttgac gcttccgcaa ggttggctaa cccaacatcc gctgggtaaa gagattattg
    37381 ctcaggaaag ccagtggcag agctatgtcc actggccgct ggaagtgcat taatgagtaa
    37441 gtgccggatg cgatgctgac gcatcttttc cggccttgat tatttccctc gttttgccgc
    37501 catcatcgct ttcagattcg ccagatggct ttgccctttc tgcatccgtt cttcggcgct
    37561 gaccactttg cgctcctgtt cccaaatcag atcatcctgc ggcagctcca gcaaaaagcg
    37621 gctcggctcc gggcgcacca gctcgccgta ctgacggcgt tctttacaca gcgtaaaggt
    37681 caattccttc tgggcgcggg taatgccgac ataggccagc cgccgctcct catcgatatt
    37741 atcttcatcg atgctgctct ggtgcggcaa aaacccttct tccataccga ccatgtagac
    37801 ataaggaaac tccagccctt ttgacgcgtg cagagtcatc agttgcacct gatccagctc
    37861 ttcttcactc tcaccacgct ccatcatgtc gcgcaaagta aagcgcgtca ccacctgggt
    37921 gagcgtcatc ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc agctaaacag
    37981 ttggttgacg ttcttcatgc gcatttcggc ggctttcggg ctgggcgatg tttcgtacag
    38041 ccaggattca taatccatgc cgtggatcag atcgcgcacc gcggcaatcg gctcccgctc
    38101 cgccagacgc tggatttctg ccaaccagtg agtaaagcgg gtcaatgctt cataaccacg
    38161 tccgctaagc gtctgactca ggcccatatc aaagctggcg gtaaacatgc ttttattgcg
    38221 cgtcatcgcc cactcaccca gctttttcag cgtcgccggg ccaatctctc tcttcggcgt
    38281 gttaacgata cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc gcagataagc
    38341 cagcaagtcc ttgatttcag gacgagagaa aaacgacgta ccaccagaaa ttttgtacgg
    38401 gatgcggttt tgcatcagga atttttcaaa cactcgcgac tgatggttac cgcgataaag
    38461 aatagcgtaa tctttgtact gcgttttatt gacgaagtga tgcgcaatca gttcaccagt
    38521 gacgcgctcg gcttcatgtt cttcgttatt cgcgcttaaa actttaagct ctgtgccgta
    38581 acccagttcg gagaacagac gcttctcaaa gacgtgcggg ttattggcga tcaggatgtt
    38641 cgccgctttc aaaatacgtc cggaagagcg gtagttctgc tcaagcttaa tcaccttcag
    38701 cgccggaaaa tcctgactca gcagcaccag gttttgtgga cgtgcgccgc gccaggagta
    38761 gatcgactgg tcgtcatcgc ccaccacggt aaagcgcgcg cggctgccca ccagcagttt
    38821 caccagttca tactggctgg tgttggtgtc ctgatactca tccaccagca gatagcgaat
    38881 tttgttctgc cagcgctcgc ggacttcttc attgcgttgc agcagcaacg tcggcaataa
    38941 aatcagatca tcgaagtcga gaacattgca cgctttcagg tgtgcatcat acagcccata
    39001 acaatgggca aaaatacggt cccgctcgcc aatcgcactg gctgccgcct gggacggtgt
    39061 tttgagatcg ttcttccagt tagagatggt cgaaatcaac tgttgcagga gaactttgtc
    39121 atcttcaatc agcccttcgg tcaactcttt gagcaacgcc agctgatcgg tatcgtcaaa
    39181 cagcgaaaag ttcgccttca tccccagcgc cgcatactca cgcttgatga tatccagccc
    39241 cagcgtgtgg aaggttgaga tcatcagccc acgcgcctct ttgcgaccca gcgtctgtcc
    39301 gacacgctct ttcatctcgc gcgccgcctt attggtaaag gtcaccgccg cgatatgtcg
    39361 cgcctgataa ccgcaaccgc ggatcagatg ggcgatttta ttggtgatca cacgagtttt
    39421 accggaaccc gcgcccgcca gcaccaggca ggggccggta acgaattcga cagcttgttg
    39481 ttggccgggg tttagacgca taggtgtatt gctcaatctt cgaacggggg gaggattgta
    39541 gcagaatcgc atttgaatgt gaccgcgtgt aagccgctaa ccgattggct gcggatgttt
    39601 aaatcagaga gggaagaccg aaaccggagt tcatcgtgaa tccattagac ttagaaaata
    39661 tcgggtcgta acgttgtgtt attacttttt cagagtttac taaggagagg tgtactcgtg
    39721 gcgattaatt tcagtcctaa ggttggtgaa atactggatt gcaactttgg taactatcct
    39781 gttagccaga atggacagtt cagcaccacg tattacgatg gccgcattcc tcctgaaatg
    39841 ataaaaaaca ggttagtt
//
LOCUS       DAJTVJ010000022        39584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000022 DAJTVJ010000000
VERSION     DAJTVJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.022"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(45..120)
                     /locus_tag="OO448_001138"
     tRNA            complement(45..120)
                     /locus_tag="OO448_001138"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Val,seq:tac)
     gene            complement(270..345)
                     /locus_tag="OO448_001139"
     tRNA            complement(270..345)
                     /locus_tag="OO448_001139"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(310..312),aa:Lys,seq:ttt)
     gene            complement(510..1301)
                     /gene="cpoB"
                     /locus_tag="OO448_001140"
     CDS             complement(510..1301)
                     /gene="cpoB"
                     /locus_tag="OO448_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6336403.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1311..1832)
                     /gene="pal"
                     /locus_tag="OO448_001141"
     CDS             complement(1311..1832)
                     /gene="pal"
                     /locus_tag="OO448_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6336404.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1867..3159)
                     /gene="tolB"
                     /locus_tag="OO448_001142"
     CDS             complement(1867..3159)
                     /gene="tolB"
                     /locus_tag="OO448_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6336405.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3292..4524)
                     /gene="tolA"
                     /locus_tag="OO448_001143"
     CDS             complement(3292..4524)
                     /gene="tolA"
                     /locus_tag="OO448_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6336406.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4589..5017)
                     /gene="tolR"
                     /locus_tag="OO448_001144"
     CDS             complement(4589..5017)
                     /gene="tolR"
                     /locus_tag="OO448_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6336407.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5021..5713)
                     /gene="tolQ"
                     /locus_tag="OO448_001145"
     CDS             complement(5021..5713)
                     /gene="tolQ"
                     /locus_tag="OO448_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6336408.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5710..6114)
                     /gene="ybgC"
                     /locus_tag="OO448_001146"
     CDS             complement(5710..6114)
                     /gene="ybgC"
                     /locus_tag="OO448_001146"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6336409.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYVPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6264..6557)
                     /gene="ybgE"
                     /locus_tag="OO448_001147"
     CDS             complement(6264..6557)
                     /gene="ybgE"
                     /locus_tag="OO448_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6336410.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6557..6670)
                     /gene="cydX"
                     /locus_tag="OO448_001148"
     CDS             complement(6557..6670)
                     /gene="cydX"
                     /locus_tag="OO448_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6336411.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6685..7824)
                     /gene="cydB"
                     /locus_tag="OO448_001149"
     CDS             complement(6685..7824)
                     /gene="cydB"
                     /locus_tag="OO448_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6336412.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7840..9408)
                     /gene="cydA"
                     /locus_tag="OO448_001150"
     CDS             complement(7840..9408)
                     /gene="cydA"
                     /locus_tag="OO448_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6336413.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10004..10231)
                     /locus_tag="OO448_001151"
     CDS             complement(10004..10231)
                     /locus_tag="OO448_001151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336414.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(10256..12812)
                     /locus_tag="OO448_001152"
                     /pseudo
     CDS             complement(10256..12812)
                     /locus_tag="OO448_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(12830..14746)
                     /gene="mngA"
                     /locus_tag="OO448_001153"
     CDS             complement(12830..14746)
                     /gene="mngA"
                     /locus_tag="OO448_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6336415.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14915..15637
                     /gene="mngR"
                     /locus_tag="OO448_001154"
     CDS             14915..15637
                     /gene="mngR"
                     /locus_tag="OO448_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6336416.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15741..16610)
                     /gene="sucD"
                     /locus_tag="OO448_001155"
     CDS             complement(15741..16610)
                     /gene="sucD"
                     /locus_tag="OO448_001155"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6336417.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16610..17776)
                     /gene="sucC"
                     /locus_tag="OO448_001156"
     CDS             complement(16610..17776)
                     /gene="sucC"
                     /locus_tag="OO448_001156"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6336418.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17870..19087)
                     /gene="odhB"
                     /locus_tag="OO448_001157"
     CDS             complement(17870..19087)
                     /gene="odhB"
                     /locus_tag="OO448_001157"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6336419.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19102..21903)
                     /gene="sucA"
                     /locus_tag="OO448_001158"
     CDS             complement(19102..21903)
                     /gene="sucA"
                     /locus_tag="OO448_001158"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6336420.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22204..22920)
                     /gene="sdhB"
                     /locus_tag="OO448_001159"
     CDS             complement(22204..22920)
                     /gene="sdhB"
                     /locus_tag="OO448_001159"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6336421.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22936..24702)
                     /gene="sdhA"
                     /locus_tag="OO448_001160"
     CDS             complement(22936..24702)
                     /gene="sdhA"
                     /locus_tag="OO448_001160"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6336422.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24702..25049)
                     /gene="sdhD"
                     /locus_tag="OO448_001161"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24702..25049)
                     /gene="sdhD"
                     /locus_tag="OO448_001161"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6336423.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25043..25447)
                     /gene="sdhC"
                     /locus_tag="OO448_001162"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25043..25447)
                     /gene="sdhC"
                     /locus_tag="OO448_001162"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6336424.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25828..25935
                     /locus_tag="OO448_001163"
     CDS             25828..25935
                     /locus_tag="OO448_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336425.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26141..27424
                     /gene="gltA"
                     /locus_tag="OO448_001164"
     CDS             26141..27424
                     /gene="gltA"
                     /locus_tag="OO448_001164"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6336426.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27814..28380
                     /locus_tag="OO448_001165"
     CDS             27814..28380
                     /locus_tag="OO448_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6336427.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28431..30889
                     /locus_tag="OO448_001166"
                     /pseudo
     CDS             28431..30889
                     /locus_tag="OO448_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            30904..31635
                     /locus_tag="OO448_001167"
     CDS             30904..31635
                     /locus_tag="OO448_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6336428.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31632..32693
                     /locus_tag="OO448_001168"
     CDS             31632..32693
                     /locus_tag="OO448_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6336429.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            32845..33891
                     /locus_tag="OO448_001169"
     CDS             32845..33891
                     /locus_tag="OO448_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6336430.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33888..34679)
                     /gene="nei"
                     /locus_tag="OO448_001170"
     CDS             complement(33888..34679)
                     /gene="nei"
                     /locus_tag="OO448_001170"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6336431.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34715..35449)
                     /gene="pxpA"
                     /locus_tag="OO448_001171"
     CDS             complement(34715..35449)
                     /gene="pxpA"
                     /locus_tag="OO448_001171"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6336432.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35439..36371)
                     /gene="pxpC"
                     /locus_tag="OO448_001172"
     CDS             complement(35439..36371)
                     /gene="pxpC"
                     /locus_tag="OO448_001172"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6336433.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36365..37021)
                     /gene="pxpB"
                     /locus_tag="OO448_001173"
     CDS             complement(36365..37021)
                     /gene="pxpB"
                     /locus_tag="OO448_001173"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6336434.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37044..37787)
                     /gene="ybgI"
                     /locus_tag="OO448_001174"
     CDS             complement(37044..37787)
                     /gene="ybgI"
                     /locus_tag="OO448_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6336435.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38058..39539
                     /gene="dtpD"
                     /locus_tag="OO448_001175"
     CDS             38058..39539
                     /gene="dtpD"
                     /locus_tag="OO448_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6336436.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9589 a        10650 c         9632 g         9713 t
ORIGIN      
        1 tgattaaaag tcaactgctc taccaactga gctaacgacc cgagtggtgg gtgatgacgg
       61 gatcgaaccg ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc
      121 gatactgcgc tggatactac aaactacttc caccgaaccc accgtaatta gtggtgggtc
      181 gtgcaggatg actcgcttcg ctcgccctac gggccgttgc tgacgcaacg ttatcctaca
      241 cgcttaacat ctgtcttcga tgctaaaact ggtgggtcgt gcaggattcg aacctgcgac
      301 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
      361 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
      421 caacaaaaat ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
      481 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt
      541 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
      601 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt
      661 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
      721 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
      781 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
      841 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg
      901 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgccgc accgctttga
      961 tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
     1021 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
     1081 cgcagggaat caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc
     1141 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
     1201 ccgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
     1261 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
     1321 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
     1381 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
     1441 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
     1501 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
     1561 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
     1621 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
     1681 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
     1741 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
     1801 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
     1861 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
     1921 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
     1981 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
     2041 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
     2101 accgttggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
     2161 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
     2221 ctgtggacga ccggcctggt cagaagtaaa tgccaggttc tggctgtccg ggaaccaggt
     2281 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
     2341 cattacgtac aggttcaggc taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
     2401 tggcgagaat gcaggcgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
     2461 gtttgccagc gtttggataa ctagcgcgga acgaccgctt tcgaaggtca cataagccag
     2521 tttagaaccg tctggtgacc acgccggaga catcagcggc tgtggtgaac ggtgaacgac
     2581 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
     2641 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc
     2701 aaacacttca tcactggcgg tatgaccagc atagcgcagc cactgcttgt tcactttgta
     2761 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
     2821 attgtaagaa ccatccggat tcggagtgac ctgaccgaca actacagcgt caatacccag
     2881 cgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
     2941 gcgatctaac ggattaaatt taccgctgtt acgcaagtcg gcagcaacga tgccgccaat
     3001 atcttcaggt gccgcaccag ggcccgccca ctggaaagga acaacaccaa taggacgacc
     3061 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
     3121 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
     3181 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
     3241 cccgtggcaa ccggtgcctg atgttgaccg tcggaacagt caacatcgcg attacggttt
     3301 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
     3361 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
     3421 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
     3481 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
     3541 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcagacga
     3601 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
     3661 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
     3721 ttttgccgct gccgcttttt tgtctgcggc ggctttttct gccgctgctt ttgccgctgc
     3781 cgcttttttg tctgcggcgg ctttttctgc cgctgctttc ttatcagctg ctgccttatc
     3841 agcagccgct ttttctgctg cttttttgtc ggctgcagct ttctctgctg ccgctttctt
     3901 tcttgcttca gctgcagcag cttctgccgc ttccgctttc ttcttcagtg ccgcagcggc
     3961 tgcctcggct tttttctgcg cttcggctgc ggctttggcg gcttctgctt ctgctttttt
     4021 cttcgcgtct gccgccgctt tcttcgctgc ttcttccgca gctttagcat ccgcttcggc
     4081 cttcgcttta gcatctgccg ccgctttcgc tgccgcctct tccgcttgct tctgctttaa
     4141 ctcggcctgt tttgcggctt cttcagcctg ctttttctgt tcctgagcag ctaaccgctc
     4201 tttctcaagt tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc
     4261 agcctgctgt tcctttatct tgcgctgctc atcagaacgc ttcgcgcttg attcctggct
     4321 ttgcatgcgt ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga
     4381 cgaaccaccg ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagagcag
     4441 cgccgcaaat aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc
     4501 gttttgttcg gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct
     4561 ctctttcaag caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt
     4621 tcacacccgc actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt
     4681 ttgcgccacc gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg
     4741 ccaccacctg ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact
     4801 gaccaatacc agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg
     4861 attcagtagc gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca
     4921 taaagatcag caacagcacc agcagtacgt ccagcaacgg tacaatgttg atttcggact
     4981 tgagatcgcg acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc
     5041 taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat
     5101 tcagttccag tttgtttacg cgctggttga ggcggttata agccattacc gccggaattg
     5161 cggcgaacag accaattgca gtcgcaatca acgcttctgc gataccgggc gcaaccattt
     5221 gcagtgttgc ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg
     5281 taccaaacag accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg
     5341 tttccagatt ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca
     5401 ctacggcttc cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc
     5461 tgtaaaatat ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga
     5521 ggcgagagag ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcgcgcgccg
     5581 ctgcgttaag aatacgggtc cgctggataa taatggccca ggatgcgatt gaaaaaccaa
     5641 tcaaaatcaa catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat
     5701 tcatgtcagt cactgcttaa actccgcgac aatagacttg ggaagcgcac aaggcttcat
     5761 tttgagtggg tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc
     5821 gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg
     5881 tatttcgagc atatcgtcga gccgcgcagg tacgtaatat tccaccgtca ttttacgtac
     5941 cacaaaggca acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat
     6001 ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc
     6061 atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca
     6121 tcccggtaat gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga
     6181 ggtttgggaa tgagggcaag ttaagggagc gtaaaaattt atgggcctgg agcggcccat
     6241 aattttttaa agaaaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca
     6301 atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc
     6361 acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc
     6421 agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga
     6481 agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaggtc
     6541 gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa
     6601 gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga
     6661 aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc
     6721 tttggtgata cgaccgaaca ttttccagta acaccaagcg gtgtagagca gaatgatcgg
     6781 taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc
     6841 ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc
     6901 gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc
     6961 ggctttatcc atacgtgcag tcaggatggt cagcagcgga agaaccacac ccagtgccgg
     7021 aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac
     7081 cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata
     7141 accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag
     7201 cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg tacgcatttg
     7261 cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc
     7321 gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac
     7381 gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac
     7441 gaaactacca atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt
     7501 ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc
     7561 cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc
     7621 gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat
     7681 aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc
     7741 gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa
     7801 acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt
     7861 cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc
     7921 aaacttgaac attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag
     7981 caccattgag aagatgagat cgcctgcggt cagtgacgag ttcgccacag ctgtcggcag
     8041 cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac
     8101 agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc
     8161 aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagaa gcaggaagcc
     8221 acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt
     8281 ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag
     8341 cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc
     8401 ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg
     8461 ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt
     8521 tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg
     8581 atcaggaatg ccgaacagag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc
     8641 aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag
     8701 aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt
     8761 agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat
     8821 cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt
     8881 aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga
     8941 cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg acaggtttga
     9001 accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc
     9061 gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc
     9121 cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa
     9181 ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt
     9241 gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc
     9301 cataatggcc agcaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat
     9361 cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg
     9421 ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa
     9481 tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag
     9541 aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat tacgctgcaa
     9601 aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat
     9661 ttatagctaa attaccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat
     9721 gccaatttcc aagacttata cattgataaa tatcaattcc tacaaattat gttaatcatg
     9781 tttcaataag gtaactttag tgacatttat gtttaaaatg tgtgagttat aaggctgaaa
     9841 ttagtttcgg ttggcaattt aaagataaaa agagacgaag caaaaacagc acggttgacc
     9901 ttaattcttt ttttggtcac tggctaatac gacgcagagt ttcaggatta ctaataaagg
     9961 cataaatcca ccccgccgat attaacattt aattaacttg taattatatt ataattaact
    10021 cacccagggt tattattttg ttaaaaataa agccacatat acgtggcttg agagtgaaga
    10081 taaacgcaaa attacctgat gcgctacgct tgccagtgtc tacatcatct ctgcaatata
    10141 ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg gcatgaacaa
    10201 agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt gctattcagg
    10261 caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat agatttgaac
    10321 cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa gaaatcacct
    10381 cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta aacagccgta
    10441 aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat cccactgggg
    10501 gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg agcttcatcg
    10561 catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc gcctgctgag
    10621 ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga caagaaagca
    10681 gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc ccaggcctta
    10741 aaagcagatc ttctttgccc agtaagccca cgccacgcag caacgtaatg gcaaaggttt
    10801 tcttctcttc accgatgact tcaaattcac gtaacccttc gctaaagaca gccatgccgt
    10861 tacgcccttc ctgtaaggca acatagttga gcatattcca taccggaacc ggcgcttctt
    10921 tccagccttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc gttagcgaac
    10981 caaactgcgt atctgccaga acactgtcag tgttaaaagg tgtagggacc aggacacgaa
    11041 ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatacgc ctgctgttat
    11101 gactaagagt gacaaccaac acccccccta ctctgccagg cttcatgaat aatatcgcat
    11161 tgcggtttcg cgttcgctgc ggtaattacc cactcttctt ttgcgggtga atagtcatat
    11221 tcatcaccat catcagagct ttcttcaatt tgcaataccc ggtcatagcg cacaccgctg
    11281 tctttatcta ccagttgcag agaaccatcc tcattgagcg caatctgcca gaaagcattt
    11341 tccagaatcc cttcagcgtc actttttgcc gcaatcacgt tgccaggctg attcgcttca
    11401 atataaagcg tgcgatagcc catagaaggg acaatctggt tgatctgaat atcaaactcc
    11461 ataaagggat cataattgcc ataatgaaca atttgccgat cgattaggcc aggatcgatc
    11521 tcacgggcat ggcgaataaa atacggtaca ggctgaccgc gatcgtcttg tagattaaac
    11581 tggctggcgc gcagccgcac agtggtgtta ataacttctt cccgcggcca gggcatcagg
    11641 ttaaacagca cgagtttgtc ggcatcactc tgtggcatgt tatcggcaat tttgcgcatg
    11701 tagaaacgca gcagattatc cgccatgtct tcagccagtt cgaagcgggc gacaatttcg
    11761 cgatgaactt tgtcactgca acaacaaccg atactgtcgt gggcatgatt ttttaagatc
    11821 tctttccaca ttttctccag caagccgtgg tggtattcaa aacccaacgt ccaggccagc
    11881 gttgccagcg gttccagcag attaacaatc ttattttcaa tacgtgcgtg ggcaattttg
    11941 atatccatgc gcgtggaacc gatggtgcga tgtacgcgca tatatttgcc atcgataaat
    12001 tcacctttca gggtggccag actttcacgc tgcgcttcga tcttctcaaa tacctcttca
    12061 aagcggctca tcacaaactt acgttgaggg tagatctcac gtagcttatc catcacttcg
    12121 aagatatttt gctgcaatgg catctggtca tgaccattcg gcagcaaaat ctctttggtt
    12181 acagacgctt tttccagcac gtcaaaataa ctgtcgaggc gtttacgtaa tccgttttcg
    12241 tcggcaggta agtacttacc gatggcgtag cccagcggca gcacctgcgc cgtcacttcg
    12301 ctaccgtcac tgctttgcca caaaaactcg gttttatcag taccgtggcg ctccgaacat
    12361 ccgcgccaga acatagtgcg ggtaatgcca aatccattgt aaatatgcgg cagttgcccg
    12421 gacatgccaa aggaatccgg taaataacct attttcatcg gctcgccaaa cgcgaggcag
    12481 tcacgcattc cgtacatcag attacggacg atggattccg cagaaacaat cgtggtatcg
    12541 gtctgggtat accaggggcc gataatcaac ttaccggctt ctacctgttt cttcacgcgg
    12601 tctttgtttt ccggtttcac cgcgaaataa tcttcgagga tcgccgtttg cccgtcgagt
    12661 acgtaatatt tgtattcgtt gtcctgttcc agtcggcaca ggatctcttc catattattg
    12721 accagcagaa tgcgtgactc ttcggtggtg aaataccact cccgatccca atgcatatgc
    12781 ggggtgatgt gaacgcgaga taccgctttc atcgtcgttt cctttttgtt tatggcatta
    12841 cgccatcagt cagatagttg ccatgcttaa ccgcgtgacg ccgccagatc aggagaattg
    12901 cagtcgagat tgcagccccc accaatgccg caccaaacca gccaattgcc gccataacac
    12961 cgcccgcgcc attatcatga agtaaaaaga gcgagaaaat gccggcaccg ggtgtcgaaa
    13021 ggccgatatt catcgcaccg acaatagcgc ccgttaccat agagcccagc acaaacgaac
    13081 caataacccg cagcggatct tcaatcgcca tcgggatcgc cccttcggta atacctgcca
    13141 gccctaacag ccaggtggat ttcccggtct caatttcaaa ctctttaaac aggcgcggtg
    13201 cgagcatcgt ggaagcggtt acggtaaatg ccgaaaccat tttgacggag gcgaaaatgg
    13261 catacgggcc gtaaacgccg ttcgccattg cgcccaggca gaatgcataa gcggctttgt
    13321 tcactggccc tccaaggtca aaggaacaca taaaaccgag aatggcaccc agcaacagcg
    13381 cgttacttcc tgacagaccg ttcagccagg cggtaagcga gttattgatc catgcgacag
    13441 gttctcccac cacaaacagc atcagactgc ccgctcccaa cgtaccgagc accgggtaga
    13501 gataaaaagt caggaatcca ttgaatttac tgctaagacg caagtgattt ttcacccagc
    13561 gcatcaagta accggctatc aatccgccaa cgaccgcgcc gagaaacccg gagccgatca
    13621 tgttggcggc aagtccagcc gcaaagcctg gcgctaacgc cggtttatct gccagagaat
    13681 aggtggtata ggccgcgagc accggtacca tcaaaattcc gagcagcccg ccgcccagct
    13741 tgcggtacat ccacagccag gagttttctt cattaaacag atcttgtagc ccgaagattt
    13801 gcgacagtaa taccgcgacc gccagcaccg tgccccccgc gacaatcaac ggtacggcaa
    13861 aagagattcc gctcaacagt gcctgtttca gctccgtttt gacacttttc accggttgcg
    13921 tatcttgctg tacggtacgc gtctcatcgc tacgcttgag ggtaagcgct tgttggatca
    13981 acgcttctgc atggcgaatc ggctcggcaa caggcactga aagcgcggga atgccattaa
    14041 aacgctcact ctccttgatg gcgacttcag ccgcaaaaat acaggcggtt gcactattga
    14101 gttgatccgc cgttaaacgc ccttcaatgc cgttagcgcc ttgtttttca acgtaaacat
    14161 ttacgccgag tttgcgtccg gctttttcca gatattccgc agccatatag gtgtgagcaa
    14221 tacccgccgg acaggccgtt acgcagacga tagttggcgc gttggaaaaa gaggcagaag
    14281 gttgcgtgcc tcccttgtca tccagcgccg agagcaactc atcaggcgtc gtcgccgact
    14341 gtatacgcgc ccgaatctca tcatccgcaa ggcgcgtggt cagcgctgtc agcagttgca
    14401 tatgcgtagt acccgcttca ttggggggaa tcgccagcag caccactaaa tcaactgctt
    14461 ccgggccatc aacgccttcc cactgaagcg gctcgctgag tgtggcgacc gcaaacgccg
    14521 cttcttttac cgcagcagtt ttgccatgcg gcacagccaa cccttcacct aaggcggtcg
    14581 ggccaaggct ttcacggcga tacacttctt ccagaaattg ctcagtactg gaaattttcc
    14641 ccagagcagc aagacgttga gtcaacgcgt ggatggcctc ttcacggctg gtaaagcgcg
    14701 cattcagaca caacgcatcg cggtgggtca gagtcgtcag attcatcatg atcctcactt
    14761 gatcgttttt atagtcgcgc cagtgtgccc gataaaacaa taccatcatg tgatcaattt
    14821 cacttgattg aaattatttg tattgtattt gtattattta caggatgaat ttatcgggca
    14881 taatggctaa attcatatta atgagatact aaaaatggga cacaagccct tataccggca
    14941 gattgccgat cgcattcgtg aacaaattgc gcgtggcgag cttaaacctg gcgatgcgtt
    15001 acccactgaa tcagcgctac aaacagagtt tggcgtcagc cgggttacgg tgcgtcaggc
    15061 tttgcgccag ctagtcgagc agcagatcct cgaaagcatt cagggcagcg ggacttacgt
    15121 caaagaagag cgggtcaatt acgatatttt tcagttaacc agttttgatg aaaaactgtc
    15181 ggaccgtcac gtcgatacac acagtgaaat cctgatattt gaagtgattc cggctgacga
    15241 ttttcttcag caacagctac aaatcaccgc gcaggatcgt gtgtggcatg tgaagcgcgt
    15301 gcgctatcgc aagcaaaagc caatggcgct ggaagaaacc tggatgccgc ttgcgttgtt
    15361 cccggatctc acctggcagg tcatggaaaa ttcgaaatat cactttattg aagaagtgaa
    15421 gaagatggtt atcgatcgta gcgaacagga aatcattcct ctgatgccaa ccgaagagat
    15481 gagccgtctg cttaatatca gccagacaaa gccgattctt gaaaaagtat cgcgtggata
    15541 tttagttgat ggtcgggtgt ttgaatatag ccgcaacgct tttaataccg atgactataa
    15601 atttaccctg atagcgcagc gaaaatcatc gcgataagca caaaaaaggc catcatacga
    15661 tggccaacca tgtcgaaacc gacagccctt cgccattcct gtagagggaa gcggcgaggg
    15721 ctatttctta ttacagatat ttatttcaga acagttttca gtgcttcacc gatatccgcc
    15781 aggctgcgaa cggttttcac gcctgcggct tccagagcag cgaatttctc atccgcagtc
    15841 cctttcccac cagcaatgat cgcacccgcg tggcccatac gtttgccttt cggcgcagtc
    15901 acaccagcga tgtaacctac aactggcttg gtaacgtgct ctttgatgta cgctgccgct
    15961 tcttcttcag cgctaccgcc gatctcaccg atcatcacga tcgcttcggt ctgcggatct
    16021 ttttcgaaca tttcgaggat gtcgataaag ttagagcctg ggatcgggtc accgccgata
    16081 ccaacacagg tcgactgacc gaaaccgtaa tccgtggtct gtttaaccgc ttcataggtc
    16141 agtgtaccgg aacgggaaac gatacccact ttacccggtt tgtgaatgtg acccggctgg
    16201 ataccgattt tgcattcacc cggagtgata acgcccgggc agttcgggcc gatcatacga
    16261 acgccggctt cgtccagttt cactttcaca gtcagcatat ccagcgtcgg gatgccttca
    16321 gtgatggtga taatcagttt gatgcctgca tcaatagctt ccagaatgga gtctttgcag
    16381 aacggtgctg gtacgtagat aacagaagcg gtagcgccag tagcagcaac agcttcacgc
    16441 acggtgttga acaccggcag gccgaggtgg gtagtaccgc ctttacctgg ggttacgccg
    16501 ccaaccattt tagtgccgta tgcaatggcc tgttctgagt ggaaagtccc ctggctacca
    16561 gtaaagccct ggcagataac cttggtgttt ttatcaatta aaatggacat tatttcccct
    16621 ccactgcggc aacaacctgc tgagctgcat ccgtcagacc ttttgctgca ataatattca
    16681 ggccgctgtc agccagtttc ttcgcgccga gttcggcgtt gttaccttcc agacgtacca
    16741 cgaccggtac gttaacaccc acttctgcta ccgcgccgat gataccgtca gcgatcaggt
    16801 cgcaacgaac gataccgccg aagatgttaa ccagaacggc tttcactttg tcgtcagaga
    16861 ggatgatttt gaacgcttcg gttacacgtt ctttggttgc gccgccgcca acgtcaagga
    16921 agttagccgg ttcgccgccg tgcagtttaa cgatgtccat cgtacccatc gccagacctg
    16981 cgccgttaac catacaaccg atgttaccgt ccagcgcaac gtagttcagt tcccactgtg
    17041 cagcctgtgc ttcacgcgga tcttcctgcg actggtcacg catttcgcgc agatcaggct
    17101 ggcggaacag tgcgttgccg tcagcgccca gtttgccgtc gaggcaaatc agatcgccct
    17161 gtttggtgat gaccagcggg ttgatttcga tcaacgccag gtcgcgctcc aggaaaatgg
    17221 tcgccaggcc catgaagatt ttggtgaact gctgaaccag tttaccttcc agacccagtt
    17281 tgaacgccag ctcgcgtccc tgatacggca tcgggccagt cagcggatca agcgcaactt
    17341 tgtggatcag gtgcggagtt tcttccgcca ctttttcgat ttccacgccg ccttcggtgg
    17401 aggccataaa gaccacacga cgggaactac ggtctacaac ggcaccgaga tacagctctt
    17461 tagcgatatc ggtcgctgct tcaaccagaa tctggttaac cggttggcca ttggcatctg
    17521 tttgatacgt taccagacgc ttgccgagcc agttttctgc aaaagcacgg atgtcttctt
    17581 tgctgtttac aactttcaca ccgcccgctt taccgcggcc accagcgtga acctgacatt
    17641 tcactaccca cgggccggca ccgatttttg aagcggcttc ttctgcttcg cgcggagtag
    17701 tacaggcata acccaccggt gctggtaagc catagcgggc aaaaagttgt tttgcctgat
    17761 attcatgtaa gttcatgtgt tctgtccatc cttcagtaat cgttatcttt taaaccgtag
    17821 accgacaccc tatccacgtc ggtctacagt gcaggtgaag cttaaactac tacacgtcca
    17881 gcagcagacg cgtcggatct tccagcaact ctttgatcgt taccaggaag cccacggatt
    17941 cgcgaccatc gatcagacgg tgatcgtagg acagcgccag gtacatcatc ggcaggatct
    18001 caacctgacc attcaccgcc atcggacgat ctttgatagc gtgcataccc agaattgcgc
    18061 tctgcggcgg gttgatgatc ggcgtagaca tcagggaacc gaacacacca ccgttggtga
    18121 tggtgaagtt accaccggtc agatcttcaa ccgtcagctt gccgtcacgg cctttaactg
    18181 ccagctcttt gattttcttc tcgatgtctg ccatgccgag ggtatcgaca tcacgcagta
    18241 ccggcgtcac caggccgcgc ggcgtggaaa ccgccatgct gacgtcgaaa tagttgtggt
    18301 aaaccacgtc atcaccgtcg atagaagcat tcacttccgg gtaacgtttc agggcttcaa
    18361 ccaccgcttt cacgtagaag gacataaagc ccagacggat gccgtggcgt ttttcaaacg
    18421 cttcaccgta ctgcttacgc agatccataa tcggcttcat gttgacttcg ttgaacgtgg
    18481 tcagcatggc ggtggagttt ttcgcttcca gcagacgctc tgccacacgc ttacgcaggc
    18541 gagtcatcgg gacacgtttt tcactacgtg cagccagagc cggttgcgcc gccggagcag
    18601 ccgctgccgg agcagactct ttcgccgggg ctttcgccag atgtttttcc acatcttcac
    18661 gagtcagacg accacccaca ccggtgcctt taatggcgct ggcgtcgaga ttgtgttcag
    18721 ccagcaggcg acggatcgcc gggcttaacg catcgttgtt ttgctcttcc agagacgcct
    18781 gctggcgttg cgccggagtg gacgctttct cttcagattt ggcgctggtt tctttaccgg
    18841 cgctgttgcc ttcacgcagg cgaccaagga tctgacgaga cgttaccgtt gtaccttcat
    18901 cttccagaac cgcatccaga atgccgtctg ctgatgccgg tacttccagt accactttgt
    18961 cagtttcgat ttctaccagc acttcatcac gtacgactgc gtcgccgggt tttttatgcc
    19021 aggttgcgac ggtggcatcg gctacggatt caggcaggtc agggaccaga atatctacgc
    19081 tactcattgt gtatccttta tttattcgac gttcagcgcg tcattaacca gatcttgttg
    19141 ctgtttctgg tgaacggaca tataccctac cgccggagag gcggaggccg tgcggcctgc
    19201 ataacgcaga gaagccccaa acggaatcac ttcacggaaa tgatgctggc tgcagtacca
    19261 tgcgccctgg ttgagcggct cttcctggca ccagacaaaa tccttgacgt gagcaaactg
    19321 ctgcaacact tcctgcatcg ctttatgcgg gaacgggtag agttgctcga tacgcacaat
    19381 ggcgacatcg tgttgattgt tcttacgacg ctgttccagc aggtcgtaat aaaccttacc
    19441 agaacacatc actacgcgct tcacgccctt cggatcaagc tcgtcgattt caccgatggc
    19501 tggcaggaag gtgccgttcg ccagttcttc gaggctggaa accgccagcg gatgacgcag
    19561 cagggatttc ggcgacatca ctaccagcgg acgacgcatc ccgcgcagcg cctgacgacg
    19621 cagcatgtgg taaacctgtg ccggggtaga cggaacgcaa acctgcatgt tttgctcagc
    19681 acaaagttgc agataacgtt ccagacgcgc ggaggagtgc tccggcccct gcccttcgta
    19741 accgtgcggc agcaacatca ccagaccaca catccggccc catttctgtt cgccagaaga
    19801 gatgaactgg tcgataacca cctgcgcacc gttggcgaag tcaccgaact gcgcttccca
    19861 gatggtcaga gtgcgtggtt ctgcggtggc ataaccgtat tcaaacgcca gcactgcttc
    19921 ttcagacagt acggagtccc agacgcggaa cgcgccctgc ccgttatgga tgtgttgcaa
    19981 cggcgtgtaa gtggaaccgt tagactggtt gtggatcacc gcgtggcggt ggaagaaggt
    20041 accgcgaccg gagtcttcac ccgacaggcg aaccggaatg ccttcgtcaa ccagcgtggc
    20101 gtaagcgagg ttttccgcac cgccccagtc gaacagtttc tcaccggcag ccatcgcctg
    20161 gcgatcgcca taaatcttgg caacgcgaga ctgcatttca actgcttccg gcaccgtgct
    20221 gatgcgtttc gccagctcct gcaggcgctt catctcaact ttgttcgggt actcttcgtc
    20281 ccattcgtgg ttgaggtacg gcgaccaggt gaaagagtgc atgttcatcg gacgccactc
    20341 tgccactacg caatcgccag catccagcgc atcgcggtac aggttaacca tctcggtggc
    20401 atcttccagc gtcgccactt tttcctgctc cagcttgtca gcgtagattt tgcgcggtgt
    20461 cggatgtttt ttgattttct gatacatcag cggctgggtt gcgctcggct cgtcggcttc
    20521 gttgtggccg tgacggcggt agcacaccag gtcgatgaag acatcacgtt taaaggtgtt
    20581 acggaaatcg agcgccagac gggtcacaaa ggcaacggct tccggatcat ccgcgttaac
    20641 gtggaaaatc ggtgcctgaa ccatcttacc gatatcagta cagtacggtg tagaacgagc
    20701 atccagtgga ttagaggtgg tgaaaccaac ctggttgttg ataacgatac gtaccgtacc
    20761 gccaacttca taaccacgcg ctttcgacat gttcagagtt tcctgaacca cgccctgccc
    20821 ggtcactgcg gcgtcaccgt ggatggtgat tggcagcact ttgttgctgc tcggctcatc
    20881 aagtctgtcc agacgggcac gaacagaacc gataactacc gggcttacaa tctcaaggtg
    20941 agacgggtta aacgccagcg ccaggtgcac caggccgcca tcggtctgga agtcagacga
    21001 gaagcccatg tggtatttca cgtcacccgt gccgaggtgt tctttatgtt taccggcaaa
    21061 ctcgtcgaac aagtcttgcg gttttttacc cagcacgttc accagcacgt tcagacgacc
    21121 acggtgcgcc atcccaagaa ccacttcgcg ggtgccgctg ttgccagcgt ggcggatcat
    21181 ctctttaagc atcgggatta acgcgtcacc gccttccagc gagaagcgtt ttgcgccagg
    21241 gaattttgcg ccgaggtaac gttcaaggcc ttcagcggcg gtcagttcgc ttaagaagcg
    21301 ttttttctct tcgctattga aagtcgcgcg accagactcg atacgctgtt ggatccagcg
    21361 tttttcttcg gtgctggtaa tgtgcatata ctcggcacca atcgggccac agtaggtttg
    21421 cttgagggct tccagcagct cgccgagttt catgttttct ttgccgctgg caaatgaacc
    21481 gacgttgaag gtctcctgga agtctgcttc ggtcagatcg tggaaagacg gatccagatc
    21541 ggccacttta tcttgctgcc acagtcccag cggatcgaga ttcgcatgct ggtgaccacg
    21601 gaagcggtat gcgttaatga gctgcaggac tttaacctgc ttcacattgg tgtcagggtc
    21661 ggagatcgtt gaagagtaac gtgaagcgtc tttcgccagg cggcggaaat attcacgcgt
    21721 ttgagagtgg aattgatccg gtttgactcc cgtaccaggt aactgctgga acgtcgaacg
    21781 ccagttagcg tcaaccgagt caggatcggt taagaagtct tcatagagct gttctatcca
    21841 gctctggttt gcgccagaga ggtaagaaga gtccaaccag gctttcaaag cgctgttctg
    21901 catcgtgatc ccttaagcat cttttttatg cttacttcgc cgtggatact accacgcaca
    21961 gtgatgtgcg tgccggggtt cacttgcgag ctcttgtcta tgtagtggct cgcgaaggaa
    22021 cctttaaaaa ctgtcttagt gtcaggggca gtttttaaag atttcctgca tttctttgtt
    22081 acgcctgatg cgctgcgctt atcaggccta cgggtaatga cttgtaggcc ggataaggcg
    22141 ttcacgccgc atccggcact ggttgcctga tgcgacgctt gcgcgtctta tcaggcctac
    22201 ggtttacgca ttacgttgca acaacatcga cttaatatgg ccgatggcgc gcgtcgggtt
    22261 cagccccttc ggacatacac tgacgcagtt catgatgctg tgacagcgga atacgctgaa
    22321 tgcatcgctc aaaccgtcga ggcggctgtc agtctcggta tcacggctat cgatcaggaa
    22381 acgatatgcc gctaacaagc ctgccgggcc gataaactta tcgggattcc accagaaaga
    22441 cggacaagag gttgaacaac atgcgcagag aatacattca tacagcccgt cgagtttttc
    22501 gcgctgctct ggcatctgta aatgctcgcg agctggcgga ttttgtccat tattcaacag
    22561 gtaaggctta attttctcat attgcgcata gaattgtccc atgtctacca ccaaatcgcg
    22621 gatcaccggt aaacctggca gcgggcgaat cacaatcttc ttgcccggct ggttgagtgc
    22681 cgaaatcggg gtaatacagg ccagaccatt cttgccgttc atgttcagac cgtcggaacc
    22741 gcacacacct tcacggcagg agcggcggaa cgacaggctg ggatctttct ctttcagctg
    22801 gataagcgca tccagcagca tcatgtcgcg accttcttcc gcttccaggg ggtaatcctg
    22861 catacgcgga gcatcatcaa catccgggtt atagcgataa attgaaaact cgagtctcat
    22921 tttcctgtct ccgcattagt aagtacgaat cttcggcggg aatgccgggc gcagtttcgg
    22981 ttccatgttg acgcttcggc gcgtcatgga ttccgactct ggcagataca gggagtggca
    23041 cagccagttt tcatcatcac gatccgggaa gtcgaagcgg ctatgtgcgc cacggctttc
    23101 ggtacggaag ttggcagaaa cagccgttgc atacgccgtt tccatcaggt tatccagttc
    23161 caagcactca acgcgctggg tgttgaactc gctggaagtg tcatccagac gggcattttt
    23221 cagacgctcg cggatcactt tcaactgctc aagccctttc gccatcgcat caccttcacg
    23281 gaagaccgag aagttatgct gcatacattc ttgcagcgct ttacgaatcg ccaccggatc
    23341 ttcaccgtta cgattattgt tccagcggtg cagacgatcc agagatgctt caacatcaga
    23401 ctcgctggca tcgcgcaatg cgccctgttc ggcgatagac tcttgcagat gcagacctgc
    23461 cgcacgacca aagaccacca ggtccagcag cgagttgccg cccagacggt tagcgccgtg
    23521 taccgataca caagcgattt caccaacggc aaacagcccc ggaacaacca catcttcgcc
    23581 tttctcgttc accgtcagcg cctgaccggt cactttggtc ggaataccgc ccatcatgta
    23641 gtgacaggtt gggataaccg gaatcggctc tttcaccgga tcgacgtgag caaaggtacg
    23701 ggaaagctcc aggatacccg gcagacggga ttcgagaact tctttaccca ggtgatcgag
    23761 tttcagtttc gcgtgtggcc cccacggacc atcacagccg cgaccttcac ggatttcaat
    23821 catgatggaa cgcgccacca catcacggcc cgccaggtct ttggcgttcg gcgcataacg
    23881 ctccataaag cgttcgccat gtttgttcag cagataaccg ccttcaccac ggcaaccttc
    23941 ggtgaccagt acgcccgcac cggcaatgcc ggtcgggtgg aattgccaca tttccatatc
    24001 ctgcaccggt acgcctgcgc ggatagccat gccgacaccg tcgccagtgt taatgtgggc
    24061 gttagtggtg gactgataaa tacgccctgc cccgccagtc gccagcacgg tagcgcgggc
    24121 tttgaaataa accacttcac cggtttcgat gcacagtgcg gtacaaccca ccaccgcgcc
    24181 atcctggttt ttcaccagat ccagcgcata ccactcggag aaaatggtgg tgtggttttt
    24241 caggttctgc tgataaagcg tgtgcaacag tgcgtgaccg gtacggtcag ccgccgccgc
    24301 agtgcgtgcc gcctgctcgc cgccgaagtt tttcgactga ccgccaaacg gacgttgata
    24361 gatacgacca tcatcaagac gcgagaacgg caggcccatg tgttcgagtt ccagaatcgc
    24421 ttccggcccg gttttacaca tatattcaat cgcgtcctgg tcaccgatat agtccgaccc
    24481 ttttacggtg tcgtacatat gccattccca gttatcttca tgggtattac ccagcgcaac
    24541 ggtaatgccg ccttgcgcag aaacggtatg ggaacgggtc gggaagactt tagagagcag
    24601 cgcacaggtc tggccgctct gggaaatttg cagcgccgcg cgcatacctg cgccaccggc
    24661 accaatcaca actgcatcaa attctctgac tggcaatttc atcacacacc ccacaccaca
    24721 acgaatccat aaatcacgta aaccaccagt gcaacgacaa tcaccagttg cagcatcagg
    24781 cgcaatgcca gcggtttaac gtagtcggtc aacacctgcc acatgccgat ccaggcatga
    24841 atcaagatag aaaacagcgc cagcagggtg aacactttgg tgaacgcaga ggcgaagaaa
    24901 ccgatccaga cttcatatgt cagttcgcca ctggtggcga aaaaaccgac catataaatg
    24961 atgtagagcg tcaggacgat agcggtagcg cgaacgagga tgaaatcatg tacgccattg
    25021 cgtcctaatg cggaggcgtt gcttaccata cgaggactcc tgcgagaagt gaaagcacga
    25081 cagtaataac aaaggagatt ttggcggagc gtttacccgc ttcgaatgtt tcttccagat
    25141 agccaaaatc catcatcatg tggcgaatac ctacgacgac gtgatacgcc agagcggtaa
    25201 ggatgcccca catgataaat ttgacgaaga agctgcccat aatcgcggaa gcttgctcga
    25261 aaccttcagg ggaagagagg ctggtaccca gaagccacag caggatgccc actgcaacaa
    25321 aggtgatcac accggaaacg cgatggagaa tggacgctat cgccgtgatg gggaaccgga
    25381 tggtctgtag gtccagatta acaggtcttt gttttttcac atttcttatc atgaataacg
    25441 cccacatgct gttcttatta ttccctgggg actacgggca cagaggttaa ctttctgtta
    25501 cctggagacg tcgggatttc cttcctccgg tctgcttgcg ggtcagacag cgtcctttct
    25561 ataactgcgc gtcatgcaaa acactgcttc cagatgcgaa aacgacacgt tacaacgctg
    25621 ggtggctcgg gattgcaggg tgttccggag acctggcggc agtataggct gttcacaaaa
    25681 tcattacaat taacctacat atagtttgtc gggttttatc ctgaacagtg atccaggtca
    25741 cgataacaac atttatttaa tttttaatca tctaatttga caatcattca acaaagttgt
    25801 tacaaacatt gccaggaaaa gcatataatg cgtaaaagtt atgaagtcgg tatttcacct
    25861 aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac agttattagt
    25921 ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta ttcgtcatca
    25981 atggatcctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc tctttcacag
    26041 agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc aaatttaagt
    26101 tccggcagtc ttacgcaata aggcgctaag gagaccttaa atggctgata caaaagcaaa
    26161 actcaccctc aacggggaca cagctgttga actggatgtg ctgaaaggca cgctgggtca
    26221 agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg acccaggctt
    26281 cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg aaggtatttt
    26341 gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc tggaagtttg
    26401 ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat ttaaaactac
    26461 ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccatg ctttccgtcg
    26521 cgactcgcat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg cgttctatca
    26581 cgactcgctg gatgttaaca atcctcgtca ccgtgaaatt gccgcgttcc gcctgctgtc
    26641 gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc catttgttta
    26701 tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct ccacgccgtg
    26761 cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc tgatcctgca
    26821 cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct cttcgggtgc
    26881 gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg cgcacggtgg
    26941 tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac acattccgga
    27001 atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct tcggtcaccg
    27061 cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc atgaagttct
    27121 gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg aaaacatcgc
    27181 gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt tctactctgg
    27241 tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt tcgcaatggc
    27301 acgtaccgtt ggctggatcg cccactggag cgaaatgcac agtgacggta tgaagattgc
    27361 ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg atatcaagcg
    27421 ttaatggttg attgctaagt tgtaaatatt ttaacccgcc gttcaaatgg cgggttaatt
    27481 tttatatgcc caaacacaaa aaattgtaaa aataaaatcc actatcggac attcatatat
    27541 tctaaaaaaa atatatcggt ataaattata aacaaatcga tgatttttaa tcatctatct
    27601 tcctctcagt tacatgaata aaccaccaaa tatagcgcac ccctataatt tccaatactt
    27661 tattcatcaa acatttaatt cataccatca attaattact tttacattta ataaaattaa
    27721 taatattttt aagaaaaact aaaaacattc gacaatcaaa taagatattc ttgcaccgat
    27781 atactttttt cttttctgtt aaaggactca gttatgttta aaggacaaaa aacattggcc
    27841 gcactggccg tatctctgct gttcacagca cctgtttatg cggctgatga aggatccggt
    27901 gaaattcact ttaaaggtga agttattgaa gcaccgtgtg aaatacatca ggatgatatt
    27961 gataaagagg ttgaactcgg tcaggtgacc accagccaca ttaatcagtc acatcacagc
    28021 gatgccgttg ctgtcgacct gcgcttagtc aactgtgatc tggaaaactc cagcaacggt
    28081 tccggtggca agatttctaa agttgcagtc acctttgata gctcagcgaa aaccaccggc
    28141 gcagatccga ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg cgtacgttta
    28201 atgaataaag atcaaagcaa tatcgtttta ggcaccgcta ctccagatat cgacctggct
    28261 ccgacctcca gcgaacaaac gctgaatttc tttgcctgga tggaacaaat tgatcaggct
    28321 acacctgtaa cgccaggcgc agttacggcg aatgcaacct acgtgctgga ttataaataa
    28381 gtattacttt ggcggcgcta tcgccgcctt tcataaaata tttatcaggt atggacaccg
    28441 tgaatattta tcgactctct tttgtatcct gcctggtcgt ggcgatgcct tgcgcattgg
    28501 cggtcgaatt caaccttaat gttctcgata aatcgatgcg cgaccgcatt gatatttcat
    28561 tattaaagga aaaaggagtc attgctcccg gtgagtattt tgttagcgtt gcggtaaata
    28621 ataaccaaat cagtaacggg caaaagatta actggcacaa aaatgacgat aaaaccattc
    28681 cgtgcatcaa tgatttactg gtcgataaat ttggcttaaa acctgaagtc cgtcagtcgt
    28741 taccattgat aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc ttcaatttcg
    28801 atcaagccaa tcagcaactg aatattagta ttccgcaagc ctggctggcg tggcactcag
    28861 aaaactggac cccaccctcc acatggaaag aaggtgtcgc cggtatcctg atggattaca
    28921 acttgtttgc cagcagctac cgcccacagg acggcagcag cagcactaac ctgaacgcct
    28981 acggtaccac cggaattaac gccggggcat ggcgcttacg tagtgattat cagttgaatc
    29041 agactgatag cgatgataac catgaacagt caggcggaat atcgcgcacc tatctttttc
    29101 gtccattacc gcaattaggc tctaaattaa ccctcggcga aacggatttt agttccaata
    29161 ttttcgacgg tttttcttat accggcgcgg cactggcaag tgacgagcga atgttgccat
    29221 gggaactacg cggctacgcc ccacaaatta gcggtattgc acagaccaat gccacggtga
    29281 cgatcagtca atcaggccgc gtcatttacc agaaaaaagt cccaccaggc ccatttatca
    29341 ttgacgacct taatcagtct gttcagggca cactggatgt caaagtgacg gaagaagatg
    29401 gtcgggtgaa caatttccag gtttcggcag catcgacgcc cttcctgact cgtcagggac
    29461 aggttcgcta taaactggcc gcgggtcagc cacggccctc catgtcacat caaactgaaa
    29521 atgaaacctt ttttagcaat gaagtttcct gggggatgct gtcaaacacc tcgctgtacg
    29581 gcggcctgct gctttctggt gatgactacc attctgccgc aatgggtatt gggcaaaata
    29641 tgctgtggct tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat tttgatactc
    29701 agcaggacga gcggggctta agctaccgtt ttaattacag caaacaagtg gatgccacta
    29761 acagcacgat ttccctcgcc gcttatcgtt tctccgatcg tcattttcac agctacgcca
    29821 actatctgga tcacaaatac aacgacagcg atgcgcagga cgaaaaacag acgatcagct
    29881 tattgtgggc caaccgatta ccccactaaa cctcaatctt tacgccaacc tgctacatca
    29941 aacctggtgg aatgcagacg cctccacgac cgccaacatc acggcaggtt ttaatgttga
    30001 tattggtgac tggagagata tctctatttc gacgtcattc aatacaaccc attacgaaga
    30061 taaagatcac gacaaccaga tttacctgtc gatttcgctc cccttcggta acggtggtcg
    30121 ggttggctat gacatgcaaa acagtagcca cagcaccaca caccgcatgt cgtggaacga
    30181 tacgctggat gaacgtaata gctggggcat gtctgccgga ctgcaatccg accgtcctga
    30241 caatggagcc caggtgagcg gtaactatca gcacctgagt tcagcgggtg agtgggatat
    30301 ttctggtacc tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt
    30361 caccgcaacc caatatggtg cagcgtttca tcgccgcagc tccaccaatg aaccacgcct
    30421 gatggtcagc accgatggcg tggcagatat tccggttcag ggcaatctcg actacaccaa
    30481 ccattttggc attgcggtgg tgccgttgat ttccagttac cagccttcca ccgtggcggt
    30541 gaacatgaat gacttacccg acggcgtaac agttgcagaa aacgttatca aagaaacgtg
    30601 gattgaaggc gcgataggtt acaaatcact ggcttcccgt tccggtaaag acgttaacgt
    30661 catcattcgc aacgccagcg gtcagtttcc tcccctcgga gcggatatcc gccaggatga
    30721 cagcggcatt agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc
    30781 tgaaaatcaa aagtttaccg tggtctgggg tgatagccag cattgctcgc tccatcttcc
    30841 tgaacatatg gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg
    30901 aaaatgacat ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac
    30961 gctgcactac aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt
    31021 ctgcgggtgg ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat
    31081 gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc cgccgataca acgtctggaa
    31141 ccgaaagcaa caacgcaggt acggatagta aagcaagcct cgaccacaaa attaccgggc
    31201 gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc ctcccgcccc ggaaaaaaac
    31261 agcgaccatg ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt ctggcgaccg
    31321 gctgcattac gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag
    31381 ggcaaccaac tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctacctggga
    31441 cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc ttttagtacg
    31501 gtcaacacaa acaccggaaa ttatagcggc agccagtttt atctcggtta tatggatgat
    31561 tacggcgcgt tacgcatgac gacgctcaac tgcagcggac aatgccattt acaagccgtg
    31621 gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt catctgtctg ttatttctgc
    31681 cgttgaagtc tgcgcttgcc ctgaactgct actttggttc atccggtggc tcggtagaaa
    31741 aatcagaagc gattcaacct tttgctgtac caggcaatgc caaactcggc gataagattt
    31801 gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac ggcaattttg
    31861 aaagtgagca tgtttatgcc tgggtaaacc cctacccggg ggtgcaggat cgctattatc
    31921 aactcggcgt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg
    31981 acactaacca gtgtattgac agtaagaaca ttgacattta tacccctgag cagattatcg
    32041 cgatgggatg gcagaacaaa atttgctcgg gtgatcccgc taatattcac atgtcgcgta
    32101 cctttctcgc tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg catgattatc
    32161 aaagtacgct tagcgactat atcgtcgtgc aatttgacgg tgccggtagc gttaacgaag
    32221 accctactgc ccaaaacctg aaatatcata ttactggtct ggaaaacatt cgcgtgctgg
    32281 attgcagcgt caatttttcc atttcaccgg aaacacaggt gattgatttt ggtaaattta
    32341 atttgctgga tattcgtcgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat
    32401 cacaaaatga ccaatgcacc gacggattta aagtcagttc ctccttttat accgaagaaa
    32461 cgttggttga agaagataaa gcattgttga ttggtaatgg tctgaagcta cgtttgctgg
    32521 atgaaaacgc ctcgccttat accttcaata aatatgctga atacgccgat ttcaccagcg
    32581 acatgttggt ctacgaaaaa acctatacag ctgaactttc gtccattgca ggtaccccca
    32641 tcgaggctgg gcccttcgat acagtggtgc tttttaaaat taactataac tgatttgcca
    32701 tgatgccctt tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt
    32761 ccttgattgc tgttattttt tcctggcccg gctttcttta tgctgtatcg atacaccaaa
    32821 gagaaaataa tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta
    32881 tcacttctcc tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg
    32941 ccgatgatcg ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc
    33001 gcttttctcg ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg
    33061 attctcacca cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg
    33121 ctttccagcg ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc
    33181 ggtgcctggg gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac
    33241 ggcgcagata ttcgcctggt tgcatttatg caatatctgc gagtgctgtt cgtcgcgggg
    33301 gccgctgttc tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa
    33361 atcgtctggt tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt
    33421 gccggtacgg cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg
    33481 gcgggcgcag tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg
    33541 gcgatggcgt atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta
    33601 ctgcgggcac tacgcccgct accgcaaatc ctgctgtcga tttttgctct gctggctatt
    33661 tgtgcgggta tggcgtgggg gctgacccgg tttatgcaca ttgattttat gactgcctac
    33721 ctcgccacca gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc
    33781 gatatggcgc ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacgggg
    33841 ccagccattg cgcggtttat ttcaacctat gcgccgaagc ggtcggccta gtgctggcag
    33901 ccagggcacc agtaaaacgg gcgagatgac agcgtggttt tctcaatgat gctgccacag
    33961 cgttcgcacg gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc
    34021 ttattctcat ccaccagccc cctcgtagcg taggaaaatc gaggaatatc cagtaacgcg
    34081 tgtgcgagtg catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac
    34141 ccaacctgcc agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga
    34201 tcgagcagta atcctgcaaa ctgacggtta cgaaagcgcg gtgacaataa ccgttccttt
    34261 accacctccg gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga
    34321 tgcgtggtca gttgttccgg cctcaacatc tcaatatccg aggcgctata aagcagaatg
    34381 gttttgtcag ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg
    34441 gtatcaacca cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taagtcgttg
    34501 gaaaaatgag ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata
    34561 agttgtgatt gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg
    34621 cctttgatcg ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg
    34681 gtgatatcct tttttcattc tttattaatc ggggttatgc tgcgacaaca atctcctttt
    34741 cggcaaatgt agagcgtagt cggcgggcga acgccagtgc gtgctcgccg tcgccatgca
    34801 ggcagactgt ttgcgccgtg accgttgccc attcgccggt gatacttttg actctgccgt
    34861 gttgcaccat ctctagcgtt tgcgccaacg cctgttcttc gttttcaatc aacgcgcctc
    34921 gctgacttcg cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct
    34981 cgcgcgttgt caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa
    35041 cgagaatcaa tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg
    35101 cttctttcgc cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc
    35161 cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg
    35221 tttctggcgg cagctgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt
    35281 gagcgccaat cgcgacacca ttttttattg cttcacgcac gcaagcctgc atgatttgcg
    35341 catcgcctgc atgaaatcca caggcaatat tggcagagga aaccagcgtt aatagctctg
    35401 cgtcgctggc gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc
    35461 cacactaatt gttcgaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt
    35521 gaacactgga caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca
    35581 gcctcaatga tacaggcaat acgcgggtaa ccatcggtgg tctgtgcgtc gttcatcaac
    35641 acaatcggct gcccgttatg tggcacctgc accacgcccg gtaacaaacc gtgagataac
    35701 agttcgcgat cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc catgcggtta
    35761 ctttgcgagc taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac
    35821 tcatgatatt ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg
    35881 ccctgcgctt ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc accatccttc
    35941 agtaaacggc cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt
    36001 accggcggaa catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg
    36061 cgtttaagcg ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggca
    36121 ttatcatcca gccgcgcttc gcaaccggca cccgtcagag caaaccaccc atcagtttcg
    36181 aactcaaccg ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc
    36241 accagcaggt tagcaatgcg taacgcaggc atatccagtg cgccgcagtg gctgattccc
    36301 gactggcgaa aaccgtgacg accgccatcc tgcacagtgg tatacatgcc cgcacgaata
    36361 atcttcagca tactccctcc ttctgcggta caaagcgcac gctgtctccc ggacgtaata
    36421 agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc
    36481 agccacccgg cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac
    36541 ctgccggaac gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac
    36601 tcccgagata cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg
    36661 agtgcaattc aacaacctgt ttttcgctaa acccgcaatg cgccgcgacc accgccaaat
    36721 ccggtccgcc tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca
    36781 gggcctcgct ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact
    36841 caggattacg caaaatcacc gtgatattgt tcatgccggg aatggcttca accacattcg
    36901 gcatatccac cagacgctgc gccagtcgcc agatccgttt ctggctagcc agcgtcaccg
    36961 gcggttccag ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca
    37021 ctttcgatcc ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca
    37081 agatcggtat tttcattcag ccactcgctc aatgcgcgaa taccaccacg ttcagtggca
    37141 tggtgacctg cagcataaaa atgcaacccc tgctcgcggg ctgaatgaat ggtctgttca
    37201 gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt
    37261 tgcccgccgc ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac
    37321 cataacggct tacgtcccag acgcgcttca atccaggagg ctagttccag tcccggcaca
    37381 ggcatggtca gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc
    37441 agtaacgccg ccagctgtgc gttattgccc agctcaggat gtacgtcaag cggcaaatgc
    37501 cagccataca ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg
    37561 cgaatgaccg gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg
    37621 cccagacgca ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt
    37681 tgcaccgtct ctttgccttc tacctgcaaa ccattcggcg cgtaatcact aatcgccgcg
    37741 ctgttcagtt tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc
    37801 gtattctgat agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc
    37861 ttttgacata ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg
    37921 cttcagctaa tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta
    37981 tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgcag
    38041 tcaattgtgg aattattatg aataaacacg catcacaacc gcgcactatt tactatgtcg
    38101 ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct
    38161 atctcaccaa tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact
    38221 gttcgctggt gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca
    38281 atcgcatggc ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg gtgctgggtg
    38341 ccagtgagat ccatccgtca ttcctctatc tgtccctggc gattatcgtt tgtggctatg
    38401 gtctgtttaa atccaatgtc agttgcctgc tcggcgagct gtatgagcca accgatccgc
    38461 gtcgtgatgg cggtttctcg ctgatgtatg cagcgggtaa cgtggggtct attatcgcac
    38521 ctatcgcctg tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg
    38581 cggtgggtat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcacacata
    38641 cccgcggcgt taataaaaaa gtactgcgtg caacaaactt tctcctgcct aactggggat
    38701 ggctgctggt tctgctggtg gcaacgcctg cgctgattac cgtactgttc tggaaagagt
    38761 ggtcggtata cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt
    38821 atcgcaaagc agaaaaccag aaacagcgta aggagctggg gctgattgtg acgctcacct
    38881 tcttcagtat gttgttctgg gccttcgcac aacagggcgg cagctcaatt agcctttata
    38941 tcgaccgctt cgttaaccgc gatatgtttg gttataccgt tccgactgct atgttccagt
    39001 cgattaatgc cttcgcagtt atgctatgcg gtgtgttcct ggcgtgggtg gttaaagaaa
    39061 gcgtcgcagg taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc cttggcctga
    39121 tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact
    39181 cttctctgcc actgatggtg ttaggcctcg cggtgatggg ctttgcggaa ctgtttatcg
    39241 acccggttgc catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc ggcgtattaa
    39301 ccggcatcta catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg
    39361 atcagacatc gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat
    39421 atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtactga
    39481 tgatttggct gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcataat
    39541 aggccagcac cgatgcctga tgcgacgctt gacgcgtctt atca
//
LOCUS       DAJTVJ010000023        39412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000023 DAJTVJ010000000
VERSION     DAJTVJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.023"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..266
                     /locus_tag="OO448_001176"
     CDS             <1..266
                     /locus_tag="OO448_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6336437.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="OO448_001177"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="OO448_001177"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6336438.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="OO448_001178"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="OO448_001178"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6336439.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="OO448_001179"
     CDS             complement(1570..3270)
                     /locus_tag="OO448_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR6336440.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="OO448_001180"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="OO448_001180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6336441.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="OO448_001181"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="OO448_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6336442.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="OO448_001182"
     CDS             4828..6600
                     /locus_tag="OO448_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6336443.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="OO448_001183"
     CDS             6593..8374
                     /locus_tag="OO448_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6336444.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="OO448_001184"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="OO448_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6336445.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="OO448_001185"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="OO448_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6336446.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OO448_001186"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="OO448_001186"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6336447.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="OO448_001187"
     CDS             11336..11908
                     /locus_tag="OO448_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6336448.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="OO448_001188"
     CDS             complement(11939..12250)
                     /locus_tag="OO448_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6336449.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="OO448_001189"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO448_001189"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="OO448_001190"
     CDS             12629..12982
                     /locus_tag="OO448_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6336450.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OO448_001191"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="OO448_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="OO448_001192"
     CDS             complement(14736..15206)
                     /locus_tag="OO448_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6336451.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OO448_001193"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="OO448_001193"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6336452.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OO448_001194"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="OO448_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6336453.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OO448_001195"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="OO448_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6336454.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="OO448_001196"
     CDS             complement(17208..20357)
                     /locus_tag="OO448_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6336455.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OO448_001197"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="OO448_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6336456.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="OO448_001198"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="OO448_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6336457.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="OO448_001199"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="OO448_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6336458.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OO448_001200"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="OO448_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6336459.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OO448_001201"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="OO448_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6336460.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="OO448_001202"
     CDS             26836..27213
                     /locus_tag="OO448_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6336461.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="OO448_001203"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="OO448_001203"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6336462.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="OO448_001204"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="OO448_001204"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6336463.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="OO448_001204"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="OO448_001205"
     CDS             30024..30353
                     /locus_tag="OO448_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6336464.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="OO448_001206"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="OO448_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6336465.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="OO448_001207"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="OO448_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6336466.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="OO448_001208"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="OO448_001208"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6336467.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="OO448_001209"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="OO448_001209"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6336468.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OO448_001210"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="OO448_001210"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR6336469.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="OO448_001211"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="OO448_001211"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6336470.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="OO448_001212"
     CDS             complement(37406..39082)
                     /locus_tag="OO448_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6336471.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9527 a        10050 c        10419 g         9416 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccagaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgctg gt
//
LOCUS       DAJTVJ010000024        39353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000024 DAJTVJ010000000
VERSION     DAJTVJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.024"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(109..1449)
                     /gene="fadL"
                     /locus_tag="OO448_001213"
     CDS             complement(109..1449)
                     /gene="fadL"
                     /locus_tag="OO448_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6336472.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1821..2105
                     /locus_tag="OO448_001214"
     CDS             1821..2105
                     /locus_tag="OO448_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6336473.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2285..3595
                     /gene="fadI"
                     /locus_tag="OO448_001215"
     CDS             2285..3595
                     /gene="fadI"
                     /locus_tag="OO448_001215"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6336474.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3595..5739
                     /gene="fadJ"
                     /locus_tag="OO448_001216"
     CDS             3595..5739
                     /gene="fadJ"
                     /locus_tag="OO448_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6336475.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            5942..6427
                     /gene="sixA"
                     /locus_tag="OO448_001217"
     CDS             5942..6427
                     /gene="sixA"
                     /locus_tag="OO448_001217"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6336476.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7111..7674
                     /locus_tag="OO448_001218"
     CDS             7111..7674
                     /locus_tag="OO448_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6336477.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7756..10398
                     /gene="yfcU"
                     /locus_tag="OO448_001219"
     CDS             7756..10398
                     /gene="yfcU"
                     /locus_tag="OO448_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR6336478.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10418..11169
                     /locus_tag="OO448_001220"
                     /pseudo
     CDS             10418..11169
                     /locus_tag="OO448_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11144..11692
                     /locus_tag="OO448_001221"
     CDS             11144..11692
                     /locus_tag="OO448_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6336479.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11689..12159
                     /locus_tag="OO448_001222"
     CDS             11689..12159
                     /locus_tag="OO448_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6336480.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12156..12680
                     /locus_tag="OO448_001223"
     CDS             12156..12680
                     /locus_tag="OO448_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6336481.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12667..13539
                     /locus_tag="OO448_001224"
     CDS             12667..13539
                     /locus_tag="OO448_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6336482.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13661..14212)
                     /gene="smrB"
                     /locus_tag="OO448_001225"
     CDS             complement(13661..14212)
                     /gene="smrB"
                     /locus_tag="OO448_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6336483.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14378..15310
                     /gene="prmB"
                     /locus_tag="OO448_001226"
     CDS             14378..15310
                     /gene="prmB"
                     /locus_tag="OO448_001226"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6336484.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15345..16430
                     /gene="aroC"
                     /locus_tag="OO448_001227"
     CDS             15345..16430
                     /gene="aroC"
                     /locus_tag="OO448_001227"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6336485.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16434..17258
                     /gene="mepA"
                     /locus_tag="OO448_001228"
     CDS             16434..17258
                     /gene="mepA"
                     /locus_tag="OO448_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6336486.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17258..18067
                     /locus_tag="OO448_001229"
     CDS             17258..18067
                     /locus_tag="OO448_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6336487.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18067..18615
                     /gene="epmC"
                     /locus_tag="OO448_001230"
     CDS             18067..18615
                     /gene="epmC"
                     /locus_tag="OO448_001230"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6336488.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18649..18927
                     /locus_tag="OO448_001231"
     CDS             18649..18927
                     /locus_tag="OO448_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6336489.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19048..21054)
                     /gene="mnmC"
                     /locus_tag="OO448_001232"
     CDS             complement(19048..21054)
                     /gene="mnmC"
                     /locus_tag="OO448_001232"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6336490.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21213..22433
                     /gene="fabB"
                     /locus_tag="OO448_001233"
     CDS             21213..22433
                     /gene="fabB"
                     /locus_tag="OO448_001233"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6336491.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22708..23886
                     /locus_tag="OO448_001234"
     CDS             22708..23886
                     /locus_tag="OO448_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6336492.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(23883..24878)
                     /gene="flk"
                     /locus_tag="OO448_001235"
     CDS             complement(23883..24878)
                     /gene="flk"
                     /locus_tag="OO448_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6336493.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            24977..26113
                     /gene="pdxB"
                     /locus_tag="OO448_001236"
     CDS             24977..26113
                     /gene="pdxB"
                     /locus_tag="OO448_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6336494.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26179..27192
                     /locus_tag="OO448_001237"
     CDS             26179..27192
                     /locus_tag="OO448_001237"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6336495.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27192..28004
                     /gene="truA"
                     /locus_tag="OO448_001238"
     CDS             27192..28004
                     /gene="truA"
                     /locus_tag="OO448_001238"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6336496.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28087..28746
                     /locus_tag="OO448_001239"
     CDS             28087..28746
                     /locus_tag="OO448_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6336497.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            28902..29816
                     /gene="accD"
                     /locus_tag="OO448_001240"
     CDS             28902..29816
                     /gene="accD"
                     /locus_tag="OO448_001240"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6336498.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            29886..31154
                     /gene="folC"
                     /locus_tag="OO448_001241"
     CDS             29886..31154
                     /gene="folC"
                     /locus_tag="OO448_001241"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6336499.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31144..31806
                     /gene="dedD"
                     /locus_tag="OO448_001242"
     CDS             31144..31806
                     /gene="dedD"
                     /locus_tag="OO448_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6336500.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32065..32553
                     /gene="cvpA"
                     /locus_tag="OO448_001243"
     CDS             32065..32553
                     /gene="cvpA"
                     /locus_tag="OO448_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6336501.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32590..34107
                     /gene="purF"
                     /locus_tag="OO448_001244"
     CDS             32590..34107
                     /gene="purF"
                     /locus_tag="OO448_001244"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6336502.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34202..34771
                     /gene="ubiX"
                     /locus_tag="OO448_001245"
     CDS             34202..34771
                     /gene="ubiX"
                     /locus_tag="OO448_001245"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6336503.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35037..35819
                     /gene="argT"
                     /locus_tag="OO448_001246"
     CDS             35037..35819
                     /gene="argT"
                     /locus_tag="OO448_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6336504.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36040..36822
                     /gene="hisJ"
                     /locus_tag="OO448_001247"
     CDS             36040..36822
                     /gene="hisJ"
                     /locus_tag="OO448_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6336505.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            36912..37598
                     /gene="hisQ"
                     /locus_tag="OO448_001248"
     CDS             36912..37598
                     /gene="hisQ"
                     /locus_tag="OO448_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6336506.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37595..38311
                     /locus_tag="OO448_001249"
     CDS             37595..38311
                     /locus_tag="OO448_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6336507.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38319..39092
                     /gene="hisP"
                     /locus_tag="OO448_001250"
     CDS             38319..39092
                     /gene="hisP"
                     /locus_tag="OO448_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6336508.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         9312 a         9647 c        10582 g         9812 t
ORIGIN      
        1 ggctgtttgc aaaactcagg aggtaagcaa tgaggacact actttcggtg aagtggggga
       61 gttaaagtca cctgctatgc aggtgacttt atccaggcga acgcgttatc agaacgcgta
      121 gttaaagtta gtaccgaaca gccaggcttt accttcagac tcgaactggt atgggccttc
      181 gttaattttc acgctctgac cgtgcatata agaaacacca acgtcgactg aagcatcttt
      241 attaaatgcg taagtcgtac ctgcactcag ccagaaacgg tcctggtccg gaatggagat
      301 agaacgattc tgagccggaa ctgggctgtc atcaaaggcg ataccggtac ggaaggtcca
      361 gttatcatcg tagtaataag tggtacccaa cgcgatgcgg taagcatctt taaagccttc
      421 atgtttctgg aacagcgtgt cgccactggt tgaggtcgct ttcagctgct ggaactgact
      481 ccagctggtg taagccaggc tatagtgaat cgcccactgt ggatcaacac ggttataacc
      541 tgacacttcc cacatttcag gcaggttcag cgtcagataa cccgattgcg ttgcgccacc
      601 tgtcgcggta ggaattggca aaccgtagtt attaaacgca cgattaagat cgctgctgta
      661 gttacctttg aagtcaattt tcacttcaga acggtaggtc agtgcatagc ggttattttt
      721 atccagttca tacaggattc cggcgttcca gccaaagccc cactggttac cgttcagatg
      781 agcgattttg gtattactgt cgataccgtt ggcggtagct gccaatgctt gcccttgagg
      841 agtttgcccg gcaggagatt gcataatctg accagcaacc aactgcccca gatcgcctgc
      901 gaaacgttca attttcgcgc gagcgtagac ggcgttgaaa ccaagaccaa agctccatgc
      961 attatttaag cgatacgcac cgcttaagtt caggttcatg gtttcaaggt cagttgtacc
     1021 cccgacagag ccgcctgcat aagtatcatt aaactctgtt gccagaccat agttagaggt
     1081 aatagaagcg ccccaaccaa attggtcgtt aatcggtgca acaaagtgca tgttcggaac
     1141 ccatgccgta ggcgcgatgt tatcggcttt caggctacga ccagacggag acgttccgct
     1201 gatattcaca tccgggtcaa tataaaccgc acccgcagaa aatgtcgggc ggtcaaacat
     1261 ggtaatcaat gcggggttac ggctaacgtt acctgcatca tcggcaattg cgccttcccc
     1321 tgaataagcc cggcccaggc cagaggaaga aaattcgttt aactgaaagc ctgccgacca
     1381 ggcctgggtg gagataagtg ccactgcgac tgcgagagca gactttgtaa acagggtttt
     1441 ctggctcatg accataacct caatgattta tttttataca aactatgtta cgtgctgtaa
     1501 caggagcgcg aagtgtaggg tctgaggtac atcttagaaa tcagaccagt ggcgagagta
     1561 taggtcggac cagctggaat gctgcaagta tgtttccatc ttttttcaaa tatgatctaa
     1621 gaaacgcgat ccagctggca aaatttggag gttgcgcact cgcggattcg ggtgaatttt
     1681 gttttagatc atttttaagt gtgatttcgg tcacttatcc gattttgcag aattaacaac
     1741 tggagcagca ctttccggaa gcgtaaaata tccacaacgt ttatctggca ccttgggtgc
     1801 acatacagag gaaatcaacg atgagtaaat gtagtgctga tgaaaccccg gtttgctgct
     1861 gtatggatgt tggcaccatt atggacaact ccgactgcac cgcgtcttac agccgcgtgt
     1921 ttgcaaaccg cgcagaagca gaacaaacgc tggcggcgct gactgaaaaa gcccgtagcg
     1981 tggaatccga accgtgcaaa atctccccga ctttcactga agagtctgac ggtgtgcgcc
     2041 tggatatcga tttcactttt gcctgcgaag ctgaaatgct gatcttccag ctgggtctgc
     2101 gttaattcct ctcgggtacg ttcgcccctg tcagttgctg gcaggggcgt tttcttttct
     2161 ttctcctgtc ttcactgtgt catagctccc actttccttt ttctccgctt ggctaaatgt
     2221 aaaaaaatag ttaagactat gatcaggtca gaccacttta tttatttttt tacaggggag
     2281 tgttatgggt caggttttac cgctggttac ccgccagggc gatcgtatcg ccattgttag
     2341 cggtttacgt acgccttttg cccgccaggc gacggctttt catggcattc ccgcggttga
     2401 tttagggaag atggtggtag gcgaactgct ggcacgcagc gagatccccg ctgaagtgat
     2461 tgaacagctg gtctttggtc aggtcgtaca aatgcctgaa gcccccaaca ttgcgcgtga
     2521 aattgttctc ggtaccggaa tgaatgtgca taccgatgct tacagcgtca gccgcgcttg
     2581 cgctaccagt ttccaggcgg ttgcaaacgt cgcagaaagc ctgatggcgg gaactatccg
     2641 agcggggatt gccggtgggg cagattcctc ttccgtattg ccaattggcg tcagtaaaaa
     2701 actggcgcgc gtgctggttg atgtcaacaa agctcgaacc atgagccagc gcctgaaact
     2761 cttctctcgc ctgcgtttgt gcgacttaat gcccgtaccg cctgcggtag cagaatattc
     2821 tactggcttg cggatgggcg acaccgccga acaaatggcg aaaacctacg gcatcacccg
     2881 agaacagcaa gatgcattag cgcaccgttc gcatcagcgt gccgctcagg catggtcaga
     2941 cggaaaactc aaagaagagg tgatgactgc ctttatccct ccttataaac aaccgcttgt
     3001 cgaagacaac aatattcgcg gtaattcctc gcttgccgat tacgcaaagc tgcgcccggc
     3061 gtttgatcgc aaacacggaa cggtaacggc ggcaaacagt acgccgctga ccgatggcgc
     3121 ggcagcggtg atcctgatga ctgaatcccg ggcgaaagaa ttagggctgg tgccgctggg
     3181 gtatctgcgc agctacgcat ttactgcgat tgatgtctgg caggacatgt tgctcggtcc
     3241 agcctggtca acaccgctgg cgctggaacg tgccggtttg acgatgagcg aactgacatt
     3301 gatcgatatg cacgaagcct ttgcagctca gacactggcg aatattcagt tgctgggtag
     3361 tgaacgtttt gctcgtgaag tactggggcg tgcacatgcc actggcgaag tggacgatag
     3421 caaatttaac gtgcttggcg gttcgattgc ttacgggcat cccttcgcgg cgaccggcgc
     3481 gcggatgatt acccagacat tgcatgaact tcgccgtcgc ggcggtggat ttggtttagt
     3541 taccgcctgt gctgccggtg ggcttggcgc ggcaatggtt ctggaggcgg aataatggaa
     3601 atggcatcag cgtttactct taatgttcgt ctggacaaca ttgctatcat caccatcgat
     3661 gtaccggatg agaaaatgaa taccctgaag gcggaatttg cctcgcaggt gcgcgccatt
     3721 attaagcaac tccgtgaaaa caaagagttg cgcggcgtgg tgtttatctc cgcgaaaccg
     3781 gacaatttta tcgccggagc agacatcaac atgattggca actgcaaaac ggcgcaagaa
     3841 gcggaagcac tggcgcggca gggccaacag ttgatggcgg agattcatgc tttgcccatt
     3901 ccggttatcg cggctattca tggcgcttgc ctgggcggtg ggctggagct ggcgctggcg
     3961 tgccacggtc gcgtttgtac tgacgatcct aaaacggtgc tcggtttgcc tgaagtacaa
     4021 cttggattgt tacccggttc aggcggcacc cagcgtttac cgcgtctgat aggcgtcagt
     4081 acagcattag agatgattct caccggaaaa caacttcggg cgaaacaggc attaaagctg
     4141 gggctggtgg atgacgttgt tccgcactcc attttgctgg aagccgctgt tgagctggcg
     4201 aagaaggatc gcccatcttc ccgccctcta cctgtacgcg agcgtattct ggcggggccg
     4261 ttaggtcgtg cgctgctgtt caaaatggtc ggcaagaaaa cagaacacaa aactcaaggc
     4321 aattatccgg cgacagaacg catcctggag gttgttgaaa cgggattagc gcagggcacc
     4381 agcagcggct atgacgctga agctcgcgcc tttggcgaac tggcgatgac gccgcaatcg
     4441 caagctttgc gtagtatctt ttttgccagt acggacgtga agaaagatcc cggtagtgat
     4501 gcaccgcctg cgccattaaa cagcgtggga attttaggtg gtggcttgat gggcggcggt
     4561 attgcttatg tcactgcttg taaagcgggg cttccggtca gaattaaaga tatcaacccg
     4621 cagggcataa atcatgcgct gaagtacagt tgggatcagc tggagggcaa agttcgccgt
     4681 cgtcatctca aagccagcga acgtgacaaa cagctggcat taatctccgg aacgacggac
     4741 tatcgcggct ttgcccatcg cgatctgatt attgaagcgg tgtttgaaaa tctcgaatta
     4801 aaacaacaga tggtggcgga agtggagcaa aattgcgccg ctcataccat ttttgcttcg
     4861 aacacgtcat ctttaccgat tggtgatatc gccgctcacg ccgcgcgacc tgagcaagtt
     4921 attggcctgc atttcttcag tcctgtggaa aaaatgccgc tggtagagat tattcctcat
     4981 gcgggtacat cggcgcaaac catcgccact acggtaaaac tggcgaaaaa acagggcaaa
     5041 acgcccattg tcgtgcgtga caaagccggg ttttacgtca atcgcatctt agcgccttac
     5101 attaatgaag ctatccgcat gctgaccgaa ggtgaacggg tagagcacat tgatgccgcg
     5161 ctagtgaaat ttggttttcc ggtaggccca atccaacttt tggatgaggt aggaatcgac
     5221 accgggacta aaattattcc tgtactggaa gccgcttatg gagaacgttt tagcgcgcct
     5281 gcaaatgttg tttcttcaat tttgaacgac gatcgcaaag gcagaaaaaa tggtcggggt
     5341 ttctatcttt atggtcagaa agggcgtaaa agcaaaaaac aggtcgatcc cgccatttac
     5401 ccgctcattg gcgcacaagg gcaggggcga ctctccgcac cgcaggttgc tgaacggtgt
     5461 gtgatgttga tgctgaatga agcagtacgt tgtgttgatg agcaggttat ccgtagcgtg
     5521 cgtgacgggg atattggcgc ggtatttggc attggttttc cgccatttct cggtggaccg
     5581 ttccgctata tcgattctct cggcgcgggc gaagtggttg caataatgca acgactggcc
     5641 acgcagtatg gttcccgttt taccccttgc gagcgtttgg tcgagatggg cgcgcgtggg
     5701 gaaagttttt ggaaaacaac tgcaactgac cttcaataag aaggtcaaag ctatatgaat
     5761 ccgcgctgaa tggcggagtg ttggtcaaaa tgtaaacgca tattgactat acttacgcca
     5821 ttgaggtaaa aaacagcgtt tcattcggtg aatggataag gcacaatgcc ggccaccgtt
     5881 ttctttctct ggtttcagat gaaagaaaac ggacgaatct ggttaacaaa agcggtgcaa
     5941 tatgcaagtt tttatcatgc gtcacggcga cgcagccctt gatgccgcca gtgattcggt
     6001 tcgtcctctg accactaatg gttgtgacga atctcgcctg atggcgaact ggctgaaagg
     6061 tcaaaaagtg gaaatcgaac gcgttctggt gagcccgttc ctgcgagccg agcaaacact
     6121 ggaagaagta ggggattgtc tgaacctgcc ttccagtgca gaagttctgc cggagttaac
     6181 gccctgcggc gatgttggtc tggtcagtgc ctatttgcag gcgttgacca atgaaggtgt
     6241 tgcttcggta ctggtgatct ctcacttacc gttagtcggg tatctggttg ccgagttatg
     6301 tccgggcgaa acgccgccta tgtttaccac ctcggccatt gccagcgtaa cgcttgatga
     6361 aagcggaaac ggcacattca actggcagat gagcccgtgt aatctgaaga tggcaaaagc
     6421 tatctgatga gttccaaatt atgcccaatg aggtagattc attgggcata attgctcatc
     6481 aggcagcgga tatctgtcaa tctcactact atcgaagact ctccccatct ataaataaaa
     6541 cactttctac tttcattcgg aaattattct tctgcgcgta tcttgatttt gcccaaagtt
     6601 acatgttgaa atgaataatg tctgtttgac actcattgtc atttattaat acagataata
     6661 gagaaaatag agttgaataa tcaggcagga tgtaattgaa acttgattaa attctggcta
     6721 tagtgtttat atattatatt gtcaaaagtg gataaatgcc aatcgcatta tatgttcatt
     6781 gtttgttttt taagctgttg atattcatta ttgatatact ttctattttt tctaaatact
     6841 tattccgtat tttttaagga tgtccttaag ataacctttc ttctgtcaat atattaatta
     6901 aatctacatt tactctttag taaaattaaa ttgcatcagg ggtgtggaaa catcactatg
     6961 cggccccaca ccaacgaata caattttcta cgacctgttc tttttggatc gcatgccgta
     7021 ttcaatggct gggtataaac attggttatt gactgaatca atgtattgca atgtttatat
     7081 ttttaattat ttatagttta aggacaaata atgagtaagt ttgttaaaac agctattgct
     7141 gcggcaatgg tgatgggcgt gttcacctct acggcaacaa tcgctgcggg taacaacggt
     7201 actgcacgtt tttacggcac cattgaagac tcagtgtgtt ctatcgttcc ggacgatcac
     7261 aaactggaag ttgatatggg tgatatcggt gccgaaaaac tgaaaaataa cggcaccacc
     7321 acgccgaaaa gtttccagat tcgtctgcaa gattgcgtat ttgatactca ggaaacaatg
     7381 accactacct ttactggtac cgtttcttct gcaaatagcg gcaattatta caccattttc
     7441 aataccgata cctgtgcggc atttaacaat gttagcctgg cgatcggtga ctctctgggt
     7501 acctcttaca aaagcggcat gggtattgac cagaaaatag tgaaagacac ttctaccaat
     7561 aaaggcaaag cgaagcagac actgaacttt aaagcctggc tggtcggcgc agctgatgcg
     7621 ccagatctgg gtaatttcga agcaaatacc accttccaga ttacttacct gtaaggcata
     7681 cccccatagc gacactgcta tgggggattt aaatggattt tgaccgaaca agaataaaaa
     7741 cggatagaaa cgtgtatgcc tgaccattct ctttttcgat tacggattct tccgtggtgc
     7801 attgcgctgg caatgtcagg gagttatagc agtgtctggg ctgaagacga cattcagttt
     7861 gattcccgtt ttctggaatt aaaaggcgac acaaaaattg atctgaagcg tttttccagc
     7921 cagggatatg ttgagcccgg aaaatacaat ttacaggttc aactaaataa acagccattg
     7981 gcggaagagt acgatattta ctggtatgct ggtgaagatg acgcgagcaa aagctatgct
     8041 tgtctgacac cggaactggt agcgcagttt ggtttaaaag aagacgtggc gaaaaatctg
     8101 caatggagcc acgatgctaa atgcctgaaa tccggtcaac tggaaggcat ggaaattaag
     8161 gctgatttaa gccagtccgc attagtcatt tcactgccac aggcttacct cgaatatact
     8221 tatcccgact gggatccgcc ttcacgttgg gatgacggca tctccgggat cgtcgcggac
     8281 tacagcatca acgcacaaac ccggcacgaa gaaaatggcg gtgatgatag taacgagatc
     8341 agcggcaacg ggacggtcgg ggttaacctg gggccgtggc gtatgcgtgc tgactggcag
     8401 actaactatc aacatactcg cagtaatgat gacgatgaat tcagcggcga tgaaactcaa
     8461 aaaaaatggg agtggagtcg ctactatgcc tggcgggcgt taccatcatt aaaagccaaa
     8521 ctggcgctgg gcgaggatta cctcagatcc gatatttttg atggttttaa ctatgttggt
     8581 ggcagtgtca gtactgacga tcaaatgttg cctcccaatc tgcgcggcta cgcgccagac
     8641 atttccggcg tggcacacac cacagcaaaa gtgaccgtca gccagatggg gcgtgtgatt
     8701 tacgaaacgc aggttccggc tggaccgttt cgtattcagg atcttggtga ttctgtctcc
     8761 ggtacgttgc atattcgcat tgaagaacag aacggccagg tgcaggaata tgacatcagc
     8821 accgcctcga tgccatacct cactcgtcca ggtcaggttc gctataagat catgatgggc
     8881 cgtccgcaag agtggggaca ccatgtcgag ggtgaatttt tttctggtgc tgaagcttcc
     8941 tgggggatcg ctaacggctg gtcgttatat ggcggcgcac tgggagatga aaactatcag
     9001 tctgcggcgc ttggcgtcgg tcgcgatttg tctacattcg gcgcggtcgc gtttgatgtt
     9061 actcactcgc acaccaaact ggataaagac accgcttatg gcaaaggttc gctggacggt
     9121 aactccttcc gtgtgagtta ttccaaagac tttgaccagc tcaacagccg cgttactttt
     9181 gctggatatc gcttctcgga agagaacttt atgaccatga gcgagtatct ggatgccagt
     9241 gacagcggaa tggtacgcac gggcaacgac aaagagatgt acactgccac ttataaccag
     9301 aacttccgcg atgcgggtgt ttcggtttat ctcaactata cccgccatac ctactgggat
     9361 cgcgaggagc agacaaacta caacatcatg ctctcgcact atttcaatat aggcagtatt
     9421 cgtaatgtca gcatctcgat gactggctac cgttacgagt atgacaacca ggccgacaaa
     9481 ggcatgtaca tttcgctcag tatgccgtgg ggtgacaaca gtaccgttag ctataacggt
     9541 aactatggca gtgggacgga cagcagtcag gtcggttatt tcagccgtgt cgatgacgcg
     9601 actcactatc agttgaacgt cggcaccagt gacaaacaca ccagcgttga cggctattac
     9661 agccatgatg gttcgctggc gcaggttgac ctcagtgcga actaccatga agggcaatac
     9721 acctctgcgg gcttgtcgtt acagggcggc gcaacgctta ctgcccacgg tggcgcactt
     9781 caccgtaccc agaatatggg cgggacacgc ttgttgattg atgccgatgg cgttgccgat
     9841 gttccggtag aaggtaacgg tgctgctgtt tataccaata tgtttggtaa agccgtcgtt
     9901 tctgacgtca ataactatta ccgcaatcag gcgtatatcg acctcaacaa actgccggaa
     9961 aacgccgaag caacccagtc ggtggtacaa gccacgctaa ctgaaggggc gattggctac
    10021 cgcaaatttg ccgtcatcag tggtcaaaaa gcgatggcgg tgctgcgttt acaagacggc
    10081 agccatcctc cgtttggcgc agaagtaaaa aatgataacg agcagacagt gggccttgtc
    10141 gatgatgacg gcaatgttta tctggctggg gtgaaacctg gcgaacacat gagtgtgttc
    10201 tggagtggtg ttgcgcattg cgatatcaac ctgccggacc cgctgcctgc cgatctgttt
    10261 aacggcttgt tactgccatg ccagcataaa ggcaacgtcg cgccggttgt tcctgatgac
    10321 ataaagcctg tcattcagga gcagacgcaa caggtgacac ccaccaatcc ccctgtttct
    10381 gtttcagcta accaataacg tgattaagga atgatccatg tctgatttac tttgctctgc
    10441 caaattgggt gctacgacgc tagcgttact gcttagcgct gccagtttgt cgcccaggct
    10501 tctgtaacgc cagatcgtac ccgtttgata tttaatgaaa gcgacaaatc aatcagcgtg
    10561 acgttgcgca ataacgatcc gaaactgcct tatctggccc aaagctggat cgaagatgaa
    10621 aagggtaaca agatcagttc accgttaact gttctgccac cggtgcaacg catcgattca
    10681 atgatgaacg ggcaggtaaa agtgcaggga atgccggata tcaacaaatt gcctgccgat
    10741 cgtgaaagcc tgttctactt caacgtgcgt gagatcccgc caaaatcaaa caaagccaac
    10801 acgctgcaaa tagcactaca aacgcgtatt aagttgttct ggcgaccgaa ggcgctggag
    10861 aacgtcagca tgaaaaaccc ctggcaatat aaggttactt taacccgcaa cggtcaggaa
    10921 tttaccgtca ataacccgac gccgtattac gtcattttca gtaatgccag cacacagaaa
    10981 aatggcaatc ctgcagccgg tttctcgcca atagtgatgt cgccgaaaac ctctgcaccg
    11041 ctgaatgtga agatgggtag cgtgccggta ctgacctatg tcaacgacta tggcgctcgg
    11101 atgccgctct ttttcacttg caacggtaac agttgccagg tcgatgaaga gcagagccgc
    11161 aaaggctgag gccgggagag ccagtatgaa aggatcttgt tctgtctggg cggtaactgg
    11221 gttactgata tgttgtgttg gtagcggctt tgcagttgca gcgagtaagt cagtaagtat
    11281 gaatttacgg gtgcttgtcg atgcccctcc tccttgtacg gtaaatggtg cggcagttga
    11341 gtttggtaat gtattcatta ataagatcaa tggggttgat tataaacgtc ccattgacta
    11401 ctcgcttgtt tgtaacaatc tggcaatgga tgacctgcgt ctacagatgc aggcgacaac
    11461 ggtcgtgatt aacggtgaaa cagttatcag cacgggtatt cctgggtttg gtattcgggt
    11521 tcagaaaagt agtgatcaca caatactcga cttaacctct ggctcatggt tgccatttaa
    11581 tttttcctcg ggagttcccg ttctggaagc tgtgccggtg aaacagagtg gtacaacgtt
    11641 agcggcagct gaatttaacg ccagtgccac aattgtggtg gattaccaat gaaacggatc
    11701 tcgcttattt tgctgtgggg cttttgctcg atggcactct caaacgtctc ttttcacggt
    11761 tatttagtgc agccgcctaa ctgcacgatt agcaacgctc aaaccattga aataactttt
    11821 caggatgtgc ttattgatga cattaatggc agcaattatg agcaaaccgt gccgtattcc
    11881 attacctgcg atacggcggt tcgcgaccca ttaatggaaa tgaccttgag ctggagtggc
    11941 acactaagcg attttgataa tgctgcagta agcagcaata tcactggttt aggcattcag
    12001 ctcaaacagg cagggcagag ttttacgatc aatacgccac ttatggtgaa tgaaaccgat
    12061 ttaccggtgt taacggctgt gccagtaaaa aagagtggtg tcgttctgcc agaggcggac
    12121 tttgaggcct gggccacgtt acaggtagat taccaatgaa gaaaaagcgt acgttgtttt
    12181 ttattagtag cctgatgttg ctgggcagtg gaaccacaat agcaggagat aatttgcact
    12241 ttaccggcaa tttaatcagt aaatcgtgta cgccagtgat taatggtagc cagttggcag
    12301 aagtacattt tcctgccatt gccgccagcg atttaatgaa ccttgggcag tcagagcgag
    12361 tgccattggt gtttcaattg aaagattgtc atagttctac attgtttaat gtcaaagtaa
    12421 cgttgacagg aacagaagat agtgctctac ccggctttct ggcctttgac tcctcgtcat
    12481 cagcatccgg cgctgggatt ggtattgaaa ctgcggcagg cacatcggtg ccaatcaaca
    12541 atacaacagg cgtcacgctc ccgctgaatc agggaaataa cagcctaaat tttaatacct
    12601 ggttacaggc aaagagcggg cgagatgtaa cgtccggaga ctttagtgcc acagtgacgg
    12661 caacctttga gtatttctga aatgaaaaaa tggaccataa ttttcgcgag cctaatgctg
    12721 ctcgttctga gtgttgtcgg cgcatctaag agttatgctg gagataaact tatgagtgcg
    12781 agttttgaca gtgtgagagt ttattatgcc atggacaagg taaccggtgc tgtagcgagc
    12841 agtgttttca atgttacggt gataacgcca aaggaggtta catatgggaa atatgattct
    12901 tttgcgtata aaggcgatac gttgcgggtt atttcctggt ctgggtcggg ttcagctcca
    12961 actttggtac tgactgactt tgatacaatt aataactcaa attgccctgg catagatacg
    13021 aaaatttttc gttgtgcata tatgacgttt aaaattaccg ttgcatctga tgattatggt
    13081 tgcccttgga tcgcatcctt ttattcctat acagatttac ccggatttgg aagctatacg
    13141 gcccctacag tgcataacac aatatgccca acgataccgg tcacttcata cgatatctcc
    13201 tggagtgaaa attatgtcag ccataataag gcgttacgaa ttcagtccac ggggtcaaca
    13261 gtgaccacca cgctttcgac ctacctgatg gaaggcggca gactctgtga tggcagtaat
    13321 ttttctgata atgatggcag aggagcatac tgccgcgcgg tatcagaatt gttaaccttt
    13381 acctcttacg gttgtgataa atcaacggta acggttacgc caactcgtca tccggtaacg
    13441 gataaagtac tacacgatat tgtggtgaat gtgaatacca gtagtgggca accgattgat
    13501 tcgacgtgtc gttttcagta tgtattgaat gaactgtagt tcgtagtatt gctctgatgc
    13561 gtcttatcag gcctacgaag tgaattgcaa tttattgaat tatcacatat ttgtaggccg
    13621 gataaggcgt ttacgctgct ccggcatttg ctgccagcat tcagggcaac tccggcggca
    13681 accactcttc cacttcaatc aacaccaaca aagccgcatc accgccgtac tctttcggtg
    13741 cctggtgaaa tgccatcaca tgcggatgtt gtgccagcca cagcggtgtt tgttgcttca
    13801 aaatatgctt cccgtgccca tgcatcacgc aggcacaaaa cacatgttcg cggcgacagg
    13861 cggcaatcaa cgcgcccagt tcctgcttgg cctgcagctg cgtcagaccg tgtaaatcca
    13921 aaaacaattc cggtgaataa tcgccgcggc gcagtttctt cgcctcaaaa tggctgacat
    13981 ccgggcgaac atatttcacc ggaccttcgg tatttaataa cggctgaaac tcatcggaga
    14041 aataatggct ggcatcagcc tgctccggga tcaagcgttt caccggcact tcgctgattt
    14101 ttttacgctg cggtcggtgg acaatcgtgt cctgcttaat cttgcgagtc cccgccatca
    14161 actggcgaaa cagagcctgg tcctcctcgc taagtgttgt tttctttttc atttatctgt
    14221 ctctatttct aattttccta cagtttaccc gactcgtagc acaatcgatc gttttaaacg
    14281 tatttttcct cactcaccgc gcgtgaatgc tgatttatcg tcatcttcat ggcaaactag
    14341 ccgccgaaat tattgcgatc atgccctgga ggaatacgtg gataaaattt tcgttgatga
    14401 agcagtaaat gagctgcaaa ccattcagga catgttgcgc tggtcggtaa gccgcttcag
    14461 cgcggcaaat atctggtacg gtcacggcac cgataacccg tgggatgaag ccgtacagct
    14521 ggtgttacca tcgctctacc tgccgctgga tatcccggaa gatatgcgca ccgcgcgtct
    14581 gacctccagc gaaaaacacc gtattgttga acgcgtgatc cgccgcgtca acgagcgcat
    14641 tccggtggct tacctgacca acaaagcctg gttctgcggt catgaatttt acgtcgatga
    14701 acgcgtgctg gtgccgcgct cgccgattgg tgaactgatc aacaataaat ttgccggact
    14761 cattagcaag caaccgcagc atattttaga tatgtgtacc ggtagcggct gcatcgccat
    14821 tgcctgtgct tatgccttcc cggaagcgga agtcgacgcg gtagatatct ctcccgacgc
    14881 gctggcggtt gccgaacaga acatcgaaga acacggtctg atccacaacg tcattccgat
    14941 tcgttccgat ctgttccgtg atttgccgaa agtgcagtac gatctgattg tcactaaccc
    15001 gccgtatgtc gacgcggaag atatgtccga cctgccaaac gaataccgtc acgagccgga
    15061 actgggcctg gcatctggca ccgacggcct taaactgacg cgtcgcattc ttggaaacgc
    15121 ggcagattac cttgctgatg atggcgtgtt gatttgtgaa gtcggcaaca gcatggtaca
    15181 tcttatggaa caatatccgg atgttccgtt cacctggctg gagtttgata acggcggcga
    15241 tggtgtgttt atgctcacca aagagcagct gctggcggcc cgtgaatatt tcgcgattta
    15301 taaagattaa gtaaacacgc aaacacaaca ataacggagc cgtgatggct ggaaacacaa
    15361 ttggacaact ctttcgcgta accaccttcg gcgaatcgca tgggctggcg ctcggctgca
    15421 tcgtcgatgg tgttccgcca ggcattccgc tgacggaagc ggacctgcaa catgacctcg
    15481 accgtcgtcg ccctgggaca tcgcgctata ccacccagcg ccgcgagccg gatcaggtca
    15541 aaattctctc cggtgttttt gaaggcgtta ctactggcac cagcattggc ttgttgatcg
    15601 aaaacactga ccagcgctct caggattaca gcgcaattaa agacgttttc cgcccaggcc
    15661 atgctgatta cacctacgaa caaaaatacg gtctgcgcga ttatcgcggc ggtggacgtt
    15721 cttccgcccg cgaaaccgcc atgcgcgtgg cggcaggggc gattgccaaa aaatatctcg
    15781 ccgagaaatt tggtattgaa atccgcggct gcttgaccca gatgggcgac attccgctgg
    15841 aaatcaaaga ctggtcgctg gttgaacaaa atccgttctt ctgcccggac ccggacaaaa
    15901 tcgacgcgtt agatgaactg atgcgcgcgc tgaaaaaaga gggcgactcc atcggcgcga
    15961 aagtcaccgt tgttgccagt ggtgtccccg ccggacttgg cgagccggtc tttgaccgcc
    16021 tggatgccga catcgcccat gcgctgatga gcatcaacgc ggtgaaaggc gtggaaattg
    16081 gcgacggttt tgacgtagtg gcgctgcgcg gcagccagaa ccgcgacgaa atcaccaaag
    16141 acggtttcca gagcaaccat gcgggcggca ttctcggcgg tatcagcagc gggcagcaaa
    16201 tcattgctca tatggcgctg aaaccgacct ccagcattac cgtgccgggg cgcactatta
    16261 accgctttgg cgaagaagtt gagatgatca ccaaaggccg tcacgatccc tgtgtcggga
    16321 tccgtgcagt gccgatcgct gaagcgatgc tggcgatcgt tttaatggat cacctgttac
    16381 ggcagcgggc gcaaaatgcc gatgtgaaga ctgatattcc acgctggtaa aaaatgaaca
    16441 aaaccgcgat tgcgctgctg gctctgcttg ccagtagcgc tagcctggca gcgacgccgt
    16501 ggcaaaaaat aacccaacct gtgccgggta gcgcacaatc gataggcagt ttttctaatg
    16561 gctgtattgt cggcgctgac acgttgccga tacagtccga acattatcag gtcatgcgta
    16621 ccgatcagcg tcgctatttc ggtcacccgg atctggtgat gtttatccag cgtctgagta
    16681 gccaggtgag caatctgggc atgggtacgg tgctgattgg cgatatgggg atgcccgctg
    16741 gtgggcgttt caacggcggt catgccagcc accagaccgg actggatgtc gatatctttc
    16801 tgcaactgcc gaaaactcgc tggacctccg cgcagctctt gcgcccgcaa gcactggact
    16861 tagtttcccg cgacggtaaa cacgttgtct ccacgctgtg gaagccagaa attttcagct
    16921 tgatcaaact cgctgcccag gacaaagacg tcacgcgcat ttttgttaat ccggcgatta
    16981 aacaacaact ttgccttgat gcgggcaccg atcgcgactg gttgcgcaaa gtgcgaccct
    17041 ggttccagca tcgcgcgcat atgcatgtac gattacgttg ccctgccgat agtctggagt
    17101 gtgaagatca acctttaccg ccatcaggcg atggttgcgg ggcagaactg caaagctggt
    17161 ttgcacctct aaaaccggga acaacaaagc ctgagaagaa gacaccgcct ccgttgccgc
    17221 cttcctgcca ggcgctactg gatgagcacg tgatctaatg gaaacgttta atagcctgtt
    17281 tatggtttcc ccgctgttgc tgggagttct cttttttgtc gccatgctgg cgggatttat
    17341 cgactcgatt gccggtggcg gtgggttact caccattccg gcattgatgg ctgcgggaat
    17401 gtctcccgct aacgcgctgg caaccaataa actgcaagcc tgcggcggct ctatttccgc
    17461 cactatctac tttattcgcc gcaaagtggt tagtttaagc gatcagaaac tcaatattgc
    17521 catgaccttt gtcggctcaa tgagcggcgc actgttggta cagtacgttc aggctgatgt
    17581 cttacggcag attttgccca ttctggtgat ttgtatcggc ctctatttct tgctgatgcc
    17641 aaagctgggt gaagaagatc gccagcgccg gatgtacggt ttacccttcg cgttaattgc
    17701 cggtggctgc gtcggttttt atgacggatt ctttggcccg gctgccggat cgttttacgc
    17761 gctggccttc gttacgctgt gcggattcaa cctcgccaaa gccacggctc acgccaaatt
    17821 actcaacgca acgtcaaaca ttggcggtct gctgctgttt attctcggcg gcaaagtgat
    17881 ttgggcgacg ggctttgtca tgctggtggg gcagttcctc ggcgcgcgca tgggttcacg
    17941 gctggtgttg agcaaaggac aaaagctgat ccgcccgatg attgttatcg tctcggcggt
    18001 gatgagtgcc aaattacttt acgatagcca cggacaggag atcctccact ggttggggat
    18061 gaactaatga acagtacaca ccactatgag cagttgattg aaatttttaa tagctgcttt
    18121 gccgatgaat ttaatacccg tctgattaaa ggcgacgacg aaccgatcta tcttcctgct
    18181 gatgcggaag taccgtataa ccgaattgtc tttgcccacg gcttttatgc cagcgcaatt
    18241 catgagattt cgcactggtg tattgccgga aaagcgcgcc gtgagctggt tgatttcggc
    18301 tactggtatt gcccggatgg gcgtgatgcc caaacgcaaa gccagtttga agatgttgaa
    18361 gtgaagccgc aggcactcga ctggttgttc tgtgtggcgg cgggatatcc gtttaacgtc
    18421 agttgcgaca atctggaagg cgacttcgaa ccggatcgtg ttgtgtttca acggcgggta
    18481 catgcgcagg tgatggatta tctgacaaat ggcattcccg agcgtccggc acgcttcatt
    18541 aaagcactac aaaattatta tcacacgccg gaacttacgg cggaacagtt cccgtggccg
    18601 gaagcgctca actgaagccg gtgccacgtt ttacaatgag gaaagaagat gatcgcggag
    18661 tttgaatcac gcattctggc attaatcgac ggcatggttg accatgccag tgatgatgag
    18721 ttgtttgcca gtgggtattt gcgtggccac ctgacattag ccatcgcaga actggaaagt
    18781 ggtgatgacc actccgctca ggcggtgcat acgaccgtta gccagagtct ggaaaaagcc
    18841 attggtgccg gtgaattgtc gccgcgtgac caggcgctgg tgaccgatat gtgggaaaac
    18901 ctgtttcagc aggcgtcaca gcagtaagtt gcggcgtctg gagcaccatg cctgatgcga
    18961 cgcttatcgc gtcttatcag gcctacacgt cacacgtagg tcggataagg cgttcacgcc
    19021 gcatccggca aacactgcga gagcagatta ccccgcctta accgctttac ccttcaacaa
    19081 tttccgcacc cataaccgat tcgggtttaa cgccgtcagc gtactggcat ccatcggaat
    19141 cggttcgtcg ctcatctgcg ccgccagaat ctcggcacac agcggggcag aacacaaacc
    19201 gcgagaacct aaagccgcaa acataaagag atcgtcaaaa accggcgcgc ttaccgcctc
    19261 atctttctgc tccgccaacg acgcatattc cacgagtgtt gcctcataat cgggaacatt
    19321 gcctaccatt ggcagatgat cgcgggtggc acaacgcaca ccgcagcgcg cctctttatc
    19381 actgacatca acctcttttg cccactgtgc ctgcgggaaa caatcaatca accgctggcg
    19441 attctgctgc tgatcgtcct cactgtacgc cgtatcttcg ctgccgcgat gataactggc
    19501 accaatacaa tgatgttgat tcgccggatt ttgtggcgtg agataaccgt catagcacag
    19561 cacctgcttc agctctgcca gttccggcgt tgtcggaata tggctgacct gcccggcaac
    19621 cgaatacacc gggagagtcg acgtttggct gaaacggctg atttgatgcc cgttcgccag
    19681 taccactacg ctgtgtgttg cttgctgatc tcctgcaaaa ttcaacaacc aacagtcatc
    19741 cttacgggat aaattctgta actgatattg ataataaatc tgcaaaccct gctgttgcgc
    19801 cagttccagc acattacggg tcagttctgc tgggcacagc caaccacctt gcggataagt
    19861 aatgccgctg caatttgtcg caacgcccgt aatttgttca accgcatttg cctcaacggc
    19921 tacagccagt tctgcgggta aatccattga caacatctgt gcgattttat gctggctttt
    19981 ctcatcccag cctaactgcg tgacgccgca ccagtcatga tcaaatttaa cgggtaattg
    20041 gtcgtaaaac cgacgagcaa aagtaaacgc attagagaaa aagcggttta gcgcctcatc
    20101 gtgtttgctt aataacggat acagcgcccc ctggcgattg ccggaagcac ccagcgcggg
    20161 ggcttcatcc gcgcaataaa gcgctacctg ccagccgcgc cgtaataacg ccagcgacaa
    20221 caacgcgctg gcaataccac cgccgataat cgctacttcc cgtttgctgc tgcccgtacg
    20281 attaaaccac ggcgtggagc aggggagtgg taatgtctgt tccatcaccc cacaaagcat
    20341 ttcccgtttg cgcccaaagc ccttacgttt ttgcatcgtg aatccggctt cctgcaaacc
    20401 gcggcggaca aaaccggcag acgtaaatgt cgccagcgtg ccgcctggac gcgccaacct
    20461 tgccatggcg ttaaacagat tttgcgtcca catatccggg tttttagctg gcgcaaagcc
    20521 gtccagaaac caggcatcta ctttttgatt tagcgaatcg tccagttggc tggtcagttc
    20581 gttaatatcg ccaaaccata aatccagcgt cacgcggcct tcattgagca ataaacgatg
    20641 gcaaccgggc aagggcattg gccactgcgc ctgaagttgt tctgcccacg gagccagttc
    20701 cggccagtgt tgatgcgcta aggctaaatc cgcacgggtg aggggaaatt tctcaaaact
    20761 aatgaaatgt aagcgttgta attgcgcttg cggatgcgct tcgcgaaact gatcaaatgc
    20821 ctgccatagc gtcaggaagt ttaatccggt gccgaagccg ctctctgcta ccacaaacag
    20881 aggatgtgga tgctcaggaa agcgtacctc taattggttg cctcccagaa aaacataacg
    20941 cgtctcttcc agcccgttat cgttggaaaa atagacatcg tcaaaatctc gggaaacagg
    21001 tgtaccctca gcattaaatt cgaggttggc aggttgtatg gagtagtgtt tcacgtaagt
    21061 tactcgtctt acaggcggtg gctcgatctt agcgatgtgt gtaaggctgc gcaaatttct
    21121 ctattaaatg gctgatcgga cttgttcggc gtacaagtgt acgctattgt gcattcgaaa
    21181 cttactctat gtgcgactta cagaggtatt gaatgaaacg tgcagtgatt actggcctgg
    21241 gcattgtttc cagcatcggt aataaccagc aggaagtcct ggcatctctg cgtgaaggac
    21301 gttcagggat cactttctct caggagctga aggattccgg catgcgtagc cacgtctggg
    21361 gcaacgtaaa actggatacc actggcctca ttgaccgcaa agttgtgcgc tttatgagcg
    21421 acgcatccat ttatgcattc ctttctatgg agcaggcaat cgcagatgcg ggcctttctc
    21481 cggaagctta ccagaataac ccgcgcgttg gcctgattgc aggttctggc ggcggttccc
    21541 cgcgtttcca ggtattcggc gctgacgcaa tgcgcggccc gcgcggcctg aaagcggttg
    21601 gcccgtatgt ggtcaccaaa gcgatggcat ccggcgtttc tgcctgcctc gccacgccgt
    21661 ttaaaattca tggcgttaac tactccatca gctccgcgtg tgcgacttcc gcacactgta
    21721 tcggtaacgc agtagagcag atccaactgg gcaaacagga catcgtgttt gctggcggcg
    21781 gcgaagagct gtgctgggaa atggcctgcg aattcgacgc gatgggtgcg ctgtctacta
    21841 aatacaacga caccccggaa aaagcctccc gtacttacga cgctcaccgt gacggtttcg
    21901 ttatcgctgg cggcggcggt atggtagtgg ttgaagagct ggaacacgcg ctggcgcgtg
    21961 gtgctcacat ctatgctgaa atcgttggct acggcgcaac ctctgatggt gcagacatgg
    22021 ttgctccgtc tggcgaaggc gcagtacgtt gtatgcagat ggcgatgcat ggcgttgata
    22081 ccccaatcga ttacctgaac tctcacggta cttcgactcc ggttggcgac gtgaaagagc
    22141 tggcagctat ccgtgaagtg ttcggcgata agagcccggc gatttctgca accaaagcca
    22201 tgaccggtca ctcactgggc gctgcgggtg tgcaggaagc tatctactct ctgctgatgc
    22261 tggaacacgg ctttatcgcc ccgagcatca acattgaaga gctggacgag caggctgcgg
    22321 gtctgaacat cgtgaccgaa acgaccgatc gcgaactgac cactgttatg tctaacagct
    22381 tcggcttcgg cggcaccaac gccacgctgg taatgcgcaa gctgaaagat taattcgcag
    22441 taggccggag taggtcggat aagacgcgcc agcgtcgcat ccgacgttac gcgccaatgc
    22501 ggcctccggc actaacgcaa aagggaacct gatggttccc tttttcacat cattgacaat
    22561 cgccaccagt tccaggcaaa cttcccgctt tgtcgatttc cttctgaaaa gacgtacgcg
    22621 ttaaatcctg ccaacgcacc gtaaccctga aaccagagag atgagacggg gatactcctc
    22681 gccttgcgct gcattctgga gtaatgcatg actgctgtaa gccaaaccga aacacgatct
    22741 tctgccaatt tttcgctctt ccgcatcgct tttgcggttt ttctcaccta catgaccgta
    22801 gggctgccgt tgccggttat cccgctgttt gttcatcatg aactgggcta tggcaatacc
    22861 atggtcggca ttgccgtcgg gattcagttt ctggctacgg tgctgacgcg tggctatgcc
    22921 gggcgactgg ccgatcaata tggcgcaaaa cgttcggcgc ttcaggggat gttagcttgt
    22981 ggtctggctg gcggcgcgtt gctgctggcg gcgattttgc ctgtctccgc accgttcaaa
    23041 tttgccctgt tggtcatcgg gcgtttgatt cttgggtttg gtgaaagcca gttactgaca
    23101 ggcgctctga cctgggggtt aggcatcgta gggccaaaac actctggcaa agtgatgtca
    23161 tggaatggaa tggcgattta cggtgccctc gctgttggtg ctccgcttgg cctgttgatt
    23221 catagccatt acggttttgc cgcactggcg atcaccacaa tggtattacc cttactggcg
    23281 tgggcctgta acggcacagt gcgcaaagta ccggccctgg cgggagaacg tccatcgctg
    23341 tggagcgttg tcgggtttat ctggaaacca gggttagggc tggcactaca aggcgttggt
    23401 tttgcggtta tcgggacttt cgtttcgctc tactttgcca gcaaaggatg ggcgatggcg
    23461 ggctttactc ttaccgagtt tggcggcgca tttgtcgtga tgcgcgtcat gtttggctgg
    23521 atgccggacc gttttggcgg cgtgaaagtg gcgattgtct ctctgcttgt agaaacggtg
    23581 ggcttgttgc tgctctggca agccccaggt gcgtgggtcg cattagcggg cgcggcgtta
    23641 accggagccg gatgttcgct tatctttcct gcgctgggcg tggaggtggt taaacgcgtc
    23701 ccctcacaag ttcgcggcac cgcactgggc ggttacgccg cgtttcagga tatcgccctc
    23761 ggcgtctccg ggccgctggc gggaatgctg gcgaccacgt ttggttactc ttcggtattt
    23821 cttgccgggg cgatctctgc ggtgctggga attattgtca cgatactgtc atttcgtcgg
    23881 ggctaacgaa ccagccagac cagcatcagg ataatcgcga ctaacagtat ccacagcgcg
    23941 ggacgcgttg ccatattttg caacgtgtcc atcatcggtg gaaaaggatt aagcagcggt
    24001 tgcatatcac gcggatcaat ccctttaacc cgccgttgat aaagctgatt aagaatatcc
    24061 tcggcctggg cagaagaaag cgatgattgc gcagaaagac catatttttg ctggatatac
    24121 gccgataacg ctgccagttc actggcatct aaaggttgtt ttagcgccat ctgtagtgat
    24181 tccagcgtcg gcgtattttg ctggcttaac gtctggcgcg cctgcaacca ggtcaccaga
    24241 tggttaaaca gtttcgctgg aattaactcg ccatcgttca ccccggaaag ttccagcatc
    24301 gattgccaga tctgtttgct gggttccccc gttgccgccg caagtttggt caccagctgt
    24361 tttagcgcat tgtgctccgc cggtaataaa ggacggtcgg tcgcctcgcg ctgctgtggt
    24421 tgcggaataa ccatcttccc ttcctgcaac agggtgagaa tggtttttaa ttgctccggt
    24481 gagagctgat tcagcggcgt ctgaccaaag ttatgacgga tataatccgt gaccgcctga
    24541 cgattattcc ccagacgtaa atactcccct aattgcgcta aaagctggcg ggcagaatgg
    24601 cttttttgcg cggccagcag acgttgcgcc agattatgct cagcggcagg gaagtgacgc
    24661 gaaagcagcg gtgaatctcc cgacagacca atatcgtgcc tgatacccgc ccacagttct
    24721 gctctttgtt gttgcgtcag tgaggtcact ttcgtcatta agctttccag cgaagtacgt
    24781 tgctgactgg ataaaggctg attgcccgcg ccagacggca ggttatctcc ctgacctggt
    24841 ggttgcccag gaggagggcc ggaaataggt tgtatcatta cgtatcctta tacctgaaat
    24901 cttcgcaagt atgcctggcc gcgagattat ggcacacttg tccggttaac tctcgtctca
    24961 tacaggtaac acaaacgtga aaatccttgt tgatgaaaat atgccttatg cccgcgactt
    25021 atttagccgt ttgggtgagg tgaccgcggt tcccgggcgt ccaatccccg tcgctcaact
    25081 ggcagacgcg gatgcgctga tggtgcgttc ggtcacgaaa gtgaatgaat ctttgctggc
    25141 aggaaaaccc attaaatttg ttggcactgc cactgcgggg accgaccatg tcgatgaagc
    25201 atggttgaag caggcgggaa ttggtttttc cgctgcacct ggctgtaatg cgattgcggt
    25261 ggtggaatat gttttctcct cactactgat gcttgccgaa cgcgatggat tttcactgca
    25321 cgaccgtacg gtggggatcg tgggcgttgg taacgttgga cgtcgattgc aggcgcgact
    25381 ggaagcgtta gggattaaaa cattgctttg cgatccgcct cgcgctgacc gtggggatga
    25441 gggtgatttc cgctcgctgg atgaattagt tcagcatgcg gatattctga ctttccatac
    25501 gccactcttc aaagatgggc cgtacaaaac gctgcatctg gcggatgaaa aactgatccg
    25561 tagcctgaag ccgggagcga ttctgattaa cgcctgccgt ggcgcagtcg tcgataatac
    25621 tgcgttgctg acctgcctga atgaaggcca gaagttaagc gtagtgctgg atgtctggga
    25681 aggcgaaccg gaacttaacg tggagttgct gacaaaagtg gatatcggca cgccgcatat
    25741 cgcaggttat accctggaag gtaaagcacg cggtactacg caagtgtttg aagcctatag
    25801 caagtttatt gggcatgaac agcacgttgc gctggataca ttactgcccg cgccagagtt
    25861 tggtcgcatt acgctgcatg gcccgctcga tcaaccgacg ctgaaaaggc tggtgcattt
    25921 ggtgtatgat gtgcgccgcg atgacgcacc gctgcgtaaa gtcgccggga taccgggtga
    25981 gttcgataaa ctgcgcaaaa actatcttga gcgccgtgaa tggtcatctc tgtatgtaat
    26041 ttgtgatgac gccagtgcgg catcattgct gtgtaaactg ggttttaacg ccgttcatca
    26101 tccggcacgt taatctcttc ttcatgctct ctgctgtaac attggcaggg agctttgcta
    26161 tttctggagt aaaccaccat gtctgaaggc tggaacattg ccgtcctggg cgcaactggc
    26221 gctgtgggcg aagccctgct tgaaacgctg gctgaacgtc agttcccggt tggggaaatt
    26281 tatgcactgg cacgtaacga aagcgcaggc gaacaactgc gctttggtgg taaaacaatc
    26341 actgtgcagg atgccgctga attcgactgg acgcaggcgc agctggcatt ttttgtcgca
    26401 ggcaaagaag ctaccgctgc ctgggttgaa gaagcgacca actcaggttg cctggtgatc
    26461 gacagcagcg gactgtttgc tctcgaaccc gacgtaccgc tggtggtgcc ggaagtaaac
    26521 ccgtttgtac tgaccgatta ccgcaaccgg aatgtcatcg ccgtaccaga cagtctgacc
    26581 agccagctgc tggcggcact gaaaccgtta atcgatcagg gcggtttgtc gcgtatcagt
    26641 gttaccagcc tgatttcagc ctccgcccag ggcaaaaaag cggtcgatgc gttagcgggg
    26701 cagagtgcga aattgctcaa cggcattccg attgacgaag aagatttctt cggtcgtcaa
    26761 ctggcgttca atatgctgcc gttgttgccg gatagcgaag gtagcgtgcg tgaagaacgt
    26821 cgtatcgttg acgaagtacg caaaatcctg caggacgaag ggctgatgat ttcggcaagc
    26881 gtcgtccagg caccggtatt ctacggtcat gcccagatgg tcaactttga agcactgcgt
    26941 ccgctggcgg cagaagaagc gcgtgatgcg tttgctcagg gcgaagatat tgtgctctct
    27001 gaagagaacg aattcccgac tcaggtaggg gatgcttcgg gtacgccgca tctttctgtt
    27061 ggctgcgtgc gtaatgacta cggtatgccg gagcaagtcc agttctggtc ggtggccgat
    27121 aacgttcgct ttggcggcgc gctgatggca gtaaaaatcg ccgagaaact ggtgcaggag
    27181 tatctgtact aatgtccgac cagcaacaac tgccagttta taaaattgcg ctgggcattg
    27241 agtacgacgg cagtaagtat tacggctggc aacggcagaa tgaagtccgc agtgtgcagg
    27301 agaagctgga aaaggcgctc tcccaggtgg cgaacgaacc catcaccgtc ttctgcgccg
    27361 ggcgtactga cgcaggggta cacggtaccg ggcaggtggt gcatttcgaa acaaccgcgc
    27421 agcgcaaaga cgcggcgtgg accttgggcg taaatgcgaa tttacctggt gacatcgccg
    27481 tgcgttgggt taaagctgta cctgatgatt ttcatgcccg atttagcgcc acggctcgcc
    27541 gttatcgcta catcatctac aatcatcggc tgcgcccggc ggtattgagt aaaggggtaa
    27601 cccattttta cgaaccgctg gacgctgaac ggatgcatcg ggctgcgcaa tgcttgctgg
    27661 gcgagaatga tttcacctcg ttccgtgcgg tgcagtgcca gtcccgaacc ccgtggcgca
    27721 acgttatgca tattaatgtc acgcgtcacg gtccttatgt ggtggtagat atcaaagcga
    27781 atgcctttgt acatcatatg gtcaggaata ttgtcggcag cctgatggaa gtcggtgccc
    27841 acaaccagcc ggagagatgg atagcagagt tgctggcggc aaaggacaga acgctggcgg
    27901 cagcaacggc aaaagcggaa gggctgtatc tggtcgcggt ggattaccct gaccggtatg
    27961 atcttccaaa accgccaatg ggcccgctat ttctggcgga ctaacgagaa taatgctcgg
    28021 aacatttcag gctgtagcca acgtagttac agcctgaaag atgacgagta caaggcatag
    28081 gcaaatatgg acctgattta tttcctcatc gattttatcc tgcacattga cgtgcatctg
    28141 gcagagctgg tcgcggagta cggcgtctgg gtttatgcca ttctgttttt gattctgttc
    28201 tgcgaaacgg ggctggtggt aacgccattc ctgcctggtg attcgctgct gtttgttgct
    28261 ggtgcattgg cgtcgctgga aactaacgat ctcaatgtcc atatgatggt ggtgcttatg
    28321 ttgattgccg ctattgtcgg tgatgcagtg aactatacca ttggacggtt atttggtgac
    28381 aaactgttca gtaacccgga ttcgaaaatc tttcgtcgca gttatcttga taagactcat
    28441 cagttttacg agagacatgg cggcaaaacg attattctcg cccgttttgt accgatcgtc
    28501 agaacgttcg cgccgtttgt tgcgggaatg ggccacatgt cgtaccgtca tttcgccgct
    28561 tataacgtga tcggcgcact gttgtgggta ctgattttta cctacgcggg ttatttcttc
    28621 ggcacgctcc ccttcattca gagcaacctt aagctgatga ttgttggcat tatttttgtt
    28681 tcaatattgc ctggggtttt cgaatttatt cgtcacaaac gcgctgcggc acgcgccgca
    28741 aaataaaaag taactccgcg gttcgaccac ttttttatcc aaagtttcgg gctgttatgt
    28801 tttaatgtgc aacattcatg gtctgttggg ggcaaaaatg gcattatgcg tccccaaaga
    28861 taaaactggc atcgaaccag gttcagacag aaaggtccct aatgagctgg attgaacgaa
    28921 ttaaaagcaa cattactccc acccgcaagg cgagcattcc tgaaggggtg tggactaagt
    28981 gtgatagctg cggtcaggtt ttataccgcg ctgagctgga acgtaatctt gaggtctgtc
    29041 cgaagtgtga ccatcacatg cgtatgacag cgcgtaatcg cctgcatagc ctgttagatg
    29101 aaggaagcct tgtggagctg ggtagcgagc ttgagccgaa agatgtgctg aagtttcgtg
    29161 actccaagaa gtataaagac cgtctggctt ctgcgcagaa agaaaccggc gaaaaagatg
    29221 cgctggtggt gatgaaaggc actctgtatg gaatgccggt tgtcgctgcg gcattcgagt
    29281 tcgcctttat gggcggttca atggggtctg ttgtgggtgc acgtttcgtg cgtgccgttg
    29341 agcaggcgct ggaagataac tgcccgctga tctgcttctc cgcctctggt ggcgcacgta
    29401 tgcaggaagc actgatgtcg ctgatgcaga tggcgaaaac ctctgcggca ctggcaaaaa
    29461 tgcaggagcg cggcttgccg tacatctccg tgctgaccga cccgacgatg ggcggtgttt
    29521 ctgcaagttt cgccatgctg ggcgatctca acatcgctga accgaaagcg ttaattggct
    29581 ttgccggtcc gcgtgttatc gaacagaccg ttcgcgaaaa actgccgcca ggattccagc
    29641 gcagtgaatt cctgatcgag aaaggcgcca tcgacatgat cgtccatcgt ccggaaatgc
    29701 gcctgaaact ggcgagcatt ctggcgaagt tgatgaatct gccagcgccg aatcctgaag
    29761 cgccgcgtga aggcgtagtg gtacccccgg taccggatca ggaacctgag gcctgataac
    29821 tgataagggc agggccactg gctctgccct tttgctattc tcaccgtaac gaatcagcgg
    29881 ataccatgat tatcaaacgc actcctcaag ccgcgtcgcc tctggcttcg tggctttctt
    29941 atctggaaaa cctgcacagt aaaactatcg atctcggcct tgagcgcgtg agccaggtcg
    30001 cggcgcgtct tggcgtcctg aaaccagcgc catttgtgtt taccgttgcg ggtacgaatg
    30061 gcaaaggcac cacctgccgt acgctggagt cgattctgat ggcggcaggg tacaaagtgg
    30121 gcgtctacag ttcaccgcat ctggtgcgtt ataccgagcg cgtacgtgtg caggggcagg
    30181 aattgccgga atcggcccac accgcctctt ttgcggagat tgaatcggca cgcggtgata
    30241 tttccctgac ctatttcgag tacggtacgc tgtcggcgtt gtggctgttc aaacaggcac
    30301 aacttgacgt agtgattctg gaagtagggc tgggcggtcg tctggacgca accaatattg
    30361 tcgatgccga tgtggctgta gtaaccagca ttgcgctgga tcataccgac tggctgggac
    30421 cagatcgcga aagtattggt cgcgagaaag caggcatctt tcgcagcgca aaaccggcaa
    30481 ttgtcggtga gccggaaatg ccttctacca ttgctgatgt ggcgcaggaa aaaggtgcac
    30541 tgttacaacg tcggggcgtt gagtggaact attccgtcac cgatcatgac tgggcgttta
    30601 gcgatgctca cggcacgctg gaaaatctgc cgttgccgct tgtcccgcaa ccgaatgccg
    30661 caacagcgct ggcggcactg cgtgccagcg ggctggaagt cagtgaaaat gccattcgcg
    30721 acgggattgc cagcgcaatt ttgccgggac gtttccagat tgtgagcgag tcgccacgcg
    30781 ttatttttga tgtcgcgcat aatccacatg cggcggaata tctcaccggg cgtatgaaag
    30841 cgctaccgaa aaacgggcgc gtgctggcgg ttatcggtat gctacatgat aaagatattg
    30901 ccggaactct ggcctggttg aaaagcgtgg ttgatgactg gtattgtgcg ccactggaag
    30961 ggccgcgcgg tgccacggca gaacaactgc ttgagcattt gggtaacggc aaatcatttg
    31021 atagcgttgc gcaggcatgg gatgccgcaa tggcggacgc taaagcggaa gacaccgtgc
    31081 tggtgtgtgg ttctttccac acggtcgcac atgtcatgga agtgattgac gcgaggagaa
    31141 gcggtggcaa gtaagtttca gaatcggtta gtgggcacga tcgtgctggt ggcgctgggg
    31201 gtgattgtac ttccagggct gctggacggg cagaaaaaac attatcagga tgagtttgcg
    31261 gctatcccgc tggttccaaa agcgggcgat cgcgatgagc ctgatatgat gccagccgcc
    31321 acccaggcgt tgccgacgca gccgccggaa ggcgcagcgg aagaggtgcg ggcaggtgat
    31381 gccgcagcac cgtcgctcga tccggccact attgcagcca ataacaccga gtttgaaccg
    31441 gaacctgcac cggtcgcccc accgaagccg aaaccggtgg agccgcctaa accgaaggtt
    31501 gaagcaccac ctgcgccgaa gccagaaccg aagccggtcg tggaagaaaa agcagcgcca
    31561 acgggtaaag cctatgttgt gcaactgggt gcgctgaaaa atgccgataa agtgaatgag
    31621 attgtcggta agctgcgcgg tgccggttat cgggtttata cgtcgccatc cacgccagtg
    31681 cagggtaaaa ttacccgtat tctggttggg ccggatgcct cgaaagataa gctgaaaggt
    31741 tcgctgggtg agttgaagca actttctggc ttaagtggcg tggtaatggg ctatacgccg
    31801 aattaataca gtcttgcctg atgcgacgct ggcgcgtctt atcaggcctg ggcagtgtta
    31861 gaaccgtagg tcggataagg cgtttacgcc gcatccgaca cgcattgcac gatgccgcaa
    31921 aggcataaaa agtcgatggc gttgaatatt ttttcagcgc catttttatt gatgcgtggg
    31981 aaggaaatcc ctacgcaaac gttttctttt tctgttagaa tgcgccccga acaggatgac
    32041 agggcgtaaa atcgtgggac acatatggtc tggattgatt acgccataat cgcggtgatt
    32101 gctttttcct ctctggttag cctgatccgc ggctttgttc gtgaagcgtt atcgctggtg
    32161 acatggggtt gtgctttctt tgttgccagt cattactaca cttacctgtc agtctggttt
    32221 acgggctttg aagacgaact ggttcgaaat ggaattgcca tcgcggtact gtttatcgct
    32281 accctgattg ttggtgctat cgtgaacttc gtgataggcc agttggtgga gaaaacgggg
    32341 ttgtcaggca ccgatcgggt gctgggcgtc tgtttcggtg cgttgcgcgg tgtgttgatt
    32401 gttgccgcca ttctcttctt tctcgactcc tttaccgggg tgtcgaaaag cgaagactgg
    32461 agcaaatcac agctgatccc gcagttcagt tttatcatca gatggttttt tgattatctg
    32521 caaagctcgt caagtttctt gcccagagcg taagtgctct gagatgtggc ttaacgagga
    32581 aaaagacgta tgtgcggtat tgtcggtatc gccggtgtta tgccggttaa ccagtcgatt
    32641 tatgatgcct taacggtgct tcagcatcgc ggtcaggatg ccgccggcat catcaccata
    32701 gatgccaata actgcttccg tttgcgtaaa gcgaacgggc tggtgagcga tgtatttgaa
    32761 gctcgccata tgcagcgttt gcagggcaat atgggcattg gtcatgtgcg ttaccctacg
    32821 gctggcagct ccagcgcctc tgaagcgcag ccgttttacg ttaactcccc gtatggcatt
    32881 acgcttgccc acaacggcaa tctgaccaac gctcacgagt tgcgtaaaaa actgtttgaa
    32941 gaaaaacgcc gccacatcaa caccacttcc gactcggaaa ttctgcttaa tatcttcgct
    33001 agcgagctgg acaacttccg ccactacccg ctggaagccg acaatatttt cgctgccatt
    33061 gctgccacaa accgcttaat ccgcggcgca tatgcctgtg tggcgatgat tatcggccac
    33121 ggtatggttg ctttccgcga tccaaacggg attcgtccgc tggtactggg aaaacgtgat
    33181 attgacgaga accgtacaga atatatggtc gcttccgaaa gcgtagcgct cgatacgctg
    33241 ggctttgatt tcctgcgtga cgtcgcgccg ggcgaagcga tttacatcac tgaagaaggg
    33301 cagttgttta cccgtcaatg tgctgacaat ccggtcagca atccgtgcct gtttgagtat
    33361 gtatactttg ctcgcccgga ctcgttcatc gacaaaattt ccgtttacag cgcgcgtgtg
    33421 aatatgggca cgaaactggg cgagaaaatt gcccgcgaat gggaagatct ggatatcgac
    33481 gtggtgatcc cgatcccaga aacctcgtgt gatatcgcgc tggaaattgc tcgtattctg
    33541 ggcaaaccgt accgccaggg cttcgttaaa aaccgctatg ttggccgcac ctttatcatg
    33601 ccgggccagc agctgcgtcg taagtccgtg cgccgtaaac tgaatgccaa ccgcgccgag
    33661 ttccgcgata aaaacgtcct gctggtcgac gactccatcg tccgtggcac cacttctgag
    33721 cagattatcg agatggcacg cgaagccgga gcgaagaaag tgtacctcgc ttctgcggca
    33781 ccggaaattc gcttcccgaa cgtttatggt attgatatgc cgagcgccac ggaactgatc
    33841 gctcacggtc gcgaagttga tgaaattcgc cagatcatcg gtgctgacgg gttgattttc
    33901 caggatctga acgatctgat cgacgccgtt cgcgctgaaa atccggatat ccagcagttt
    33961 gaatgctcgg tgttcaacgg cgtctacgtc accaaagatg ttgatcaggg ctacctcgat
    34021 ttcctcgata cgttacgtaa tgatgacgcc aaagcagtgc aacgtcagaa cgaagtggaa
    34081 aatctcgaaa tgcataacga aggatgatgc cttcgctgag ggtgccggtc tggcaccctg
    34141 acttgcaact cccgccgaaa tactacaaag tctgcctgca agtctgacag ggcaactatt
    34201 tatgaaacga ctcattgtag gcatcagcgg tgccagcggc gcgatttatg gcgtgcgctt
    34261 attacaggtt ctgcgcgatg tcacagatat cgaaacgcat ctggtgatga gccaggcggc
    34321 gcgccagacc ttatccctcg aaacggattt ttccctgcgc gaagtgcagg cattagctga
    34381 tgtcacgcac gatgcgcgcg atatttccgc cagcatctct tccggttctt tccagacgct
    34441 ggggatggtg attttaccct gttcaatcaa aaccctttcc ggcattgtcc atagctatac
    34501 tgatggctta ctgacccgtg cggcagatgt ggtgctgaaa gagcgtcgcc cgttggtgct
    34561 ctgcgtgcgt gaaacaccat tgcacttagg ccatctgcgt ttaatgactc aggcggcaga
    34621 aatcggtgcg gtgattatgc ctcccgttcc ggcgttttat catcgcccgc aatcccttga
    34681 tgatgtgata aatcagacgg ttaatcgtgt tcttgaccag tttgcaataa cccttcctga
    34741 agatctcttt gcccgctggc agggcgcata actccgctgt tgccctgttt cagggcaatt
    34801 ttgcaaccgc gatcaaatcc tcgacatttt gtttctgcca ttcaatcgaa acgctgcgat
    34861 tcaaccgcta tacctgctat cttcaacttc aggacaataa tgcaacgtct tattaacata
    34921 tttaacgttg aatgttactg ttgtcgtcaa gatggcataa gacctgcatg aaagagcctg
    34981 caaacacaca acacaataca caacataaaa aagccatttt cacttgaggg ttatgtatga
    35041 agaagtcgat tctcgctctg tctttgttag tcggtctctc cgcagcggct tccagctatg
    35101 cggcgctacc ggagacggta cgtatcggaa ccgataccac ctatgcgccg ttctcgtcga
    35161 aagatgctaa aggtgatttt gttggctttg atatcgatct cggtaacgag atgtgcaaac
    35221 ggatgcaggt gaaatgtacc tgggttgcca gtgactttga cgcgctgatc ccctcactga
    35281 aagcgaaaaa aatcgacgcc attatttcgt cgctttccat taccgataaa cgtcagcagg
    35341 agattgcctt ctccgacaag ctgtacgccg cagattctcg tttgattgct gccaaaggtt
    35401 caccgattca gccaacgctg gattcactga aaggtaaaca tgttggtgtg ctgcagggat
    35461 caacccagga agcttacgct aacgagacct ggcgtagcaa aggcgtggat gtggtggcct
    35521 atgccaacca ggatttggtc tattccgatc tggctgcagg acgtctggat gctgcgttac
    35581 aagatgaagt tgctgccagc gaaggattcc tcaagcaacc tgctggtaaa gctttcgcct
    35641 ttgctggctc atcagtaaaa gacaaaaaat acttcggtga tggcaccggt gtagggctac
    35701 gtaaagatga tgctgaactg acggctgcct tcaataaggc gcttggcgag ctgcgtcagg
    35761 acggcaccta cgacaagatg gcgaaaaagt atttcgactt taatgtctac ggtgactgat
    35821 acgtcgctgg gaagctgtac ctgatggaat gatcatcatg gtgcacgcca ggtttgttgc
    35881 actatcgtgg tgcattgaaa tgcatactta agcattttta atgaaaaata atacgtctaa
    35941 cggggcggaa tattttgcct tgatggtcaa ttttatggca cgataagtgt aacaaacctg
    36001 taaatattcc ctataaaaag actgtcagtt gaggacatta tgaaaaaact ggtgctatcg
    36061 ctctctctgg ttctggcctt ctccagcgca actgcggcat ttgctgcgat tccgcaaaac
    36121 atccgcatcg gtaccgaccc gacctatgcg ccatttgaat caaaaaattc acaaggcgaa
    36181 ctggttggct tcgatatcga tctggcaaag gaattatgca aacgcatcaa tacgcaatgt
    36241 acgtttgtcg aaaatccgct ggatgcgtta atcccgtcct taaaagcgaa gaagattgac
    36301 gccatcatgt catcgctttc cattacggaa aaacgtcagc aagaaatagc cttcaccgac
    36361 aaactgtacg ctgccgattc tcgtttggtg gtggcgaaaa attctgacat tcagccgaca
    36421 gtcgagtcgc tgaaaggcaa acgggtaggc gtattgcagg gcaccaccca ggagacgttc
    36481 ggtaatgaac attgggcacc aaaaggcatt gaaatcgtct cgtatcaggg gcaggacaac
    36541 atttattctg acctgactgc cggacgtatt gatgccgcgt tccaggatga ggtcgctgcc
    36601 agcgaaggtt tcctcaaaca acctgtcggt aaagattaca aattcggtgg cccgtctgtt
    36661 aaagatgaaa aactgtttgg cgtagggacc ggcatgggcc tgcgtaaaga agataacgaa
    36721 ctgcgcgaag cactgaacaa agcctttgcc gaaatgcgcg ctgacggtac ttacgagaaa
    36781 ttagcgaaaa agtacttcga ttttgatgtt tatggtggct aatcgccatc ggtgaaagaa
    36841 agcccgctcc cttcgggtaa ccggagggag acaagaggcg gtaattcacc acacacgaca
    36901 ggacaggcag catgttgtat gggttttcag gtgttatttt acagggtgcg ctcgtcacgc
    36961 tggagctggc tatcagctct gtagtgctcg ctgtaatcat cggtttaatt ggcgctggcg
    37021 gtaagctctc gcaaaatcgg ctttcggggc tgattttcga agggtacacc acgctgattc
    37081 gtggcgtgcc ggacttagtg ttgatgctgc tgattttcta cggtttgcag attgcgctaa
    37141 acacggtgac ggaggcgatg ggcgtcgggc agattgatat cgatccgatg gtcgctggta
    37201 ttatcactct cggttttatc tacggtgctt attttaccga aacgttccgt ggtgctttta
    37261 tggcagtgcc gaaaggacat atagaggcgg cgacggcgtt cggttttact cgtgggcaag
    37321 tgtttcggcg gatcatgttt ccggcgatga tgcgttacgc cctgccaggc attggcaaca
    37381 actggcaggt gatcctcaaa tctaccgcac tggtttcgtt actcggcctg gaagatgtgg
    37441 tcaaagccac ccaactggca ggcaaaagta cctgggaacc gttctatttc gccatcgtct
    37501 gtggcgtgat ttacctggtt ttcaccactg tttccaatgg tgtgctgctg ttccttgagc
    37561 gccgctactc cgtgggtgtg aagagggctg acctgtgatc gaaatcttac atgaatactg
    37621 gaaaccgctg ctgtggaccg acggttatcg ctttactggt gtggcgatca ctctgtggct
    37681 gcttattttg tcggtagtga taggcggagt cctggcgctg tttctggcga ttggtcgtgt
    37741 ctccagtaat aaatacatcc agtttccaat ctggttattt acctatattt ttcgcggtac
    37801 gccgctgtat gttcagttgc tggtgttcta ttccggcatg tacacgcttg agattgttaa
    37861 gggaaccgaa ttccttaacg ctttcttccg cagtggcctg aactgtaccg tgctggcgct
    37921 gacgcttaac acctgcgctt acactaccga gatttttgct ggggcaatcc gttcggttcc
    37981 gcatggggaa attgaagccg ccagagccta tggcttctcg acttttaaaa tgtatcgttg
    38041 cattattttg ccttctgcgc tgcgtattgc gttaccggca tacagcaacg aagtgatcct
    38101 gatgctgcac tctactgcgc tggcgtttac ggccactgtg ccggatctgc tgaaaatagc
    38161 ccgcgatatt aacgccgcca cgtatcaacc ttttaccgcc ttcggcattg ccgcggtgct
    38221 ctatttaatc atctcttatg tcctgatcag cctctttcgc agagcggaaa aacgctggtt
    38281 gcagcatgtg aaaccttctt caacgcactg agaatacgat gtccgagaat aaattaaacg
    38341 ttatcgattt gcacaaacgc tacggcgaac atgaagtgct gaaaggggtc tcactgcaag
    38401 cgaatgccgg agatgtaata agcatcatcg gatcgtcggg atcggggaaa agtacctttc
    38461 tgcgctgcat taacttcctc gaaaaaccga gtgaagggtc gatcgtggtc aatggccaga
    38521 cgatcaatct ggtgcgcgac aaagacggtc aactcaaagt cgccgataaa aatcaactgc
    38581 gcttactgcg cacacgcctg acgatggtat tccagcactt caatctctgg agccatatga
    38641 cggtgctgga aaacgtcatg gaagcgccga ttcaggtgtt gggcctgagc aagcaggaag
    38701 cgcgcgagcg ggcggtgaag tatctggcga aagtcgggat agacgaacgt gcgcagggga
    38761 aatatccggt gcatctttcc ggcggtcagc aacagcgtgt ttctatcgcg cgggcgctgg
    38821 cgatggaacc ggaagtttta ctgtttgatg aacctacctc ggcgctcgat cctgaactgg
    38881 taggcgaagt gttacgtatt atgcagcaac tggcagaaga ggggaaaacc atggtggtag
    38941 tgactcacga aatgggcttt gctcgccatg tttctactca cgtcattttc ctccatcagg
    39001 ggaaaataga agaagagggc gcgccggagc agttatttgg caacccgcaa agccctcgtc
    39061 tgcaacagtt ccttaaggga tcgctgaaat aatttcctct ccggtggcta acgctgccgg
    39121 agtattttca tggggaattt gcgagtcagc ttccgctgaa atgtaaaagt tgcagtccgc
    39181 taaaaatact tctgaattcg ctgtccaaag ataaaaactg cctgctgcat tgggtgttaa
    39241 tcgtggttat tgtcgcgtca tcttaaattt gaggggtgac ggaacgccat gacaatatcg
    39301 acaacttcca cgccgcatga tgcggtattt aaatcttttt tacgccatcc aga
//
LOCUS       DAJTVJ010000025        39306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000025 DAJTVJ010000000
VERSION     DAJTVJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.025"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(71..283)
                     /gene="rpmE"
                     /locus_tag="OO448_001251"
     CDS             complement(71..283)
                     /gene="rpmE"
                     /locus_tag="OO448_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6336509.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            486..2684
                     /gene="priA"
                     /locus_tag="OO448_001252"
     CDS             486..2684
                     /gene="priA"
                     /locus_tag="OO448_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6336510.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2840..3865
                     /gene="cytR"
                     /locus_tag="OO448_001253"
     CDS             2840..3865
                     /gene="cytR"
                     /locus_tag="OO448_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6336511.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            3957..4916
                     /gene="ftsN"
                     /locus_tag="OO448_001254"
     CDS             3957..4916
                     /gene="ftsN"
                     /locus_tag="OO448_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6336512.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5009..5539
                     /gene="hslV"
                     /locus_tag="OO448_001255"
     CDS             5009..5539
                     /gene="hslV"
                     /locus_tag="OO448_001255"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6336513.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5549..6880
                     /gene="hslU"
                     /locus_tag="OO448_001256"
     CDS             5549..6880
                     /gene="hslU"
                     /locus_tag="OO448_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6336514.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            6947..7873
                     /gene="menA"
                     /locus_tag="OO448_001257"
     CDS             6947..7873
                     /gene="menA"
                     /locus_tag="OO448_001257"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6336515.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            7966..8451
                     /gene="rraA"
                     /locus_tag="OO448_001258"
     CDS             7966..8451
                     /gene="rraA"
                     /locus_tag="OO448_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6336516.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8536..8781)
                     /gene="zapB"
                     /locus_tag="OO448_001259"
     CDS             complement(8536..8781)
                     /gene="zapB"
                     /locus_tag="OO448_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6336517.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9207..10052
                     /gene="glpF"
                     /locus_tag="OO448_001260"
     CDS             9207..10052
                     /gene="glpF"
                     /locus_tag="OO448_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR6336518.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10075..11586
                     /gene="glpK"
                     /locus_tag="OO448_001261"
     CDS             10075..11586
                     /gene="glpK"
                     /locus_tag="OO448_001261"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6336519.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11716..12726
                     /gene="glpX"
                     /locus_tag="OO448_001262"
     CDS             11716..12726
                     /gene="glpX"
                     /locus_tag="OO448_001262"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6336520.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12823..13569
                     /gene="fpr"
                     /locus_tag="OO448_001263"
     CDS             12823..13569
                     /gene="fpr"
                     /locus_tag="OO448_001263"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6336521.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13574..14002)
                     /gene="uspD"
                     /locus_tag="OO448_001264"
     CDS             complement(13574..14002)
                     /gene="uspD"
                     /locus_tag="OO448_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6336522.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14029..14328)
                     /locus_tag="OO448_001265"
     CDS             complement(14029..14328)
                     /locus_tag="OO448_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6336523.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14540..14980)
                     /locus_tag="OO448_001266"
     CDS             complement(14540..14980)
                     /locus_tag="OO448_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6336524.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15081..15680
                     /locus_tag="OO448_001267"
     CDS             15081..15680
                     /locus_tag="OO448_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6336525.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            15788..16555
                     /gene="tpiA"
                     /locus_tag="OO448_001268"
     CDS             15788..16555
                     /gene="tpiA"
                     /locus_tag="OO448_001268"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6336526.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16610..17365)
                     /gene="cdh"
                     /locus_tag="OO448_001269"
     CDS             complement(16610..17365)
                     /gene="cdh"
                     /locus_tag="OO448_001269"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6336527.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17472..18461)
                     /gene="sbp"
                     /locus_tag="OO448_001270"
     CDS             complement(17472..18461)
                     /gene="sbp"
                     /locus_tag="OO448_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6336528.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18781..19743)
                     /gene="pfkA"
                     /locus_tag="OO448_001271"
     CDS             complement(18781..19743)
                     /gene="pfkA"
                     /locus_tag="OO448_001271"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6336529.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(19924..20826)
                     /gene="fieF"
                     /locus_tag="OO448_001272"
     CDS             complement(19924..20826)
                     /gene="fieF"
                     /locus_tag="OO448_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6336530.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(20974..21474)
                     /gene="cpxP"
                     /locus_tag="OO448_001273"
     CDS             complement(20974..21474)
                     /gene="cpxP"
                     /locus_tag="OO448_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6336531.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21624..22322
                     /gene="cpxR"
                     /locus_tag="OO448_001274"
     CDS             21624..22322
                     /gene="cpxR"
                     /locus_tag="OO448_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6336532.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22319..23692
                     /gene="cpxA"
                     /locus_tag="OO448_001275"
     CDS             22319..23692
                     /gene="cpxA"
                     /locus_tag="OO448_001275"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6336533.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23798..24472)
                     /gene="yiiM"
                     /locus_tag="OO448_001276"
     CDS             complement(23798..24472)
                     /gene="yiiM"
                     /locus_tag="OO448_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6336534.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24621..25604)
                     /gene="kdgT"
                     /locus_tag="OO448_001277"
     CDS             complement(24621..25604)
                     /gene="kdgT"
                     /locus_tag="OO448_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6336535.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25864..26484)
                     /gene="sodA"
                     /locus_tag="OO448_001278"
     CDS             complement(25864..26484)
                     /gene="sodA"
                     /locus_tag="OO448_001278"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6336536.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26769..27803
                     /gene="rhaT"
                     /locus_tag="OO448_001279"
     CDS             26769..27803
                     /gene="rhaT"
                     /locus_tag="OO448_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR6336537.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGAGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27800..28648)
                     /gene="rhaR"
                     /locus_tag="OO448_001280"
                     /pseudo
     CDS             complement(27800..28648)
                     /gene="rhaR"
                     /locus_tag="OO448_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28722..29558)
                     /gene="rhaS"
                     /locus_tag="OO448_001281"
     CDS             complement(28722..29558)
                     /gene="rhaS"
                     /locus_tag="OO448_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6336538.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29846..31315
                     /gene="rhaB"
                     /locus_tag="OO448_001282"
     CDS             29846..31315
                     /gene="rhaB"
                     /locus_tag="OO448_001282"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6336539.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31312..32571
                     /gene="rhaA"
                     /locus_tag="OO448_001283"
     CDS             31312..32571
                     /gene="rhaA"
                     /locus_tag="OO448_001283"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6336540.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            32813..33637
                     /gene="rhaD"
                     /locus_tag="OO448_001284"
     CDS             32813..33637
                     /gene="rhaD"
                     /locus_tag="OO448_001284"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6336541.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33647..33961
                     /gene="rhaM"
                     /locus_tag="OO448_001285"
     CDS             33647..33961
                     /gene="rhaM"
                     /locus_tag="OO448_001285"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6336542.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34262..34708
                     /locus_tag="OO448_001286"
     CDS             34262..34708
                     /locus_tag="OO448_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6336543.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34719..36170
                     /locus_tag="OO448_001287"
     CDS             34719..36170
                     /locus_tag="OO448_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6336544.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36160..37230
                     /locus_tag="OO448_001288"
     CDS             36160..37230
                     /locus_tag="OO448_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6336545.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37230..38978
                     /locus_tag="OO448_001289"
     CDS             37230..38978
                     /locus_tag="OO448_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR6336546.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39027..>39306)
                     /locus_tag="OO448_001290"
     CDS             complement(39027..>39306)
                     /locus_tag="OO448_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR6336547.1"
                     /translation="DAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKKYV
                     RRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9547 a        10198 c        10208 g         9353 t
ORIGIN      
        1 cagaatttat aaaaggcaaa ttacaggcac aaaaaaagcg ccgtgcggcg cttttttcgg
       61 aaatccggtc ttatttgctg cccgggatgt tgaaacgctt gttgaagcgg tcaacacggc
      121 caccggtagc aacatcacgc tgtttgccag tgaagaacgg gtggcacttg ctgcacacgt
      181 cgaggttcag gtcatgacca acggtggagc ggattttcat tacgttaccg caagagcagc
      241 tagcagtaat ttcttcgtat ttcgggtgaa tatctttttt catgggaaaa cctcggttta
      301 aggccgcgtc gctcttccag ccctaacgcc agacaccacg cgatgttaaa agtatagctt
      361 caatacgatc atttcgtacg aaggcgcgaa atcatacaga aattaaccag cgtatgcaaa
      421 ctgatccgca ctcttctacg gcaatgtgta tactaaccca ccgaatttca agtcaggatg
      481 atgctatgcc cgttgcccac gttgccttgc ccgttccgct tcctcgtacc tttgactatc
      541 tgctgccaga aggcatggcg gttaaagccg ggtgtcgcgt gcgcgtgccg tttggcaaac
      601 agcaggagcg catcggggtt gtggtatcag ttagcgatgt cagtgagctg ccgcttaacg
      661 agctgaaagc ggtagttgaa gtgctggacg ttgagccggt atttactcac tccgtctggc
      721 gattactgct ctgggcggca gattactatc atcatccgat tggcgatgtg ctgtttcatg
      781 ccttgccgat tttactgcgc caggggcggc ctgcggcgaa cgcgccgatg tggtactggt
      841 ttgccactga acaaggccag gcggtggatc tgaacagcct gaaacgctca cccaagcaac
      901 aacaggcgct ggcggcgtta cggcaaggga aaatctggcg cgaccaggtt gccacgctcg
      961 aatttaatga tgccgcgttg cagacgctgc gcaaaaaagg tctgtgtgat ttagcaagtg
     1021 aaacaccaga gtttagcgac tggcgaacga actatgccgt ttctggtgag cggttgcgat
     1081 tgaataccga acaggccacc gccgttggcg caattcatag cgcggcagat actttttctg
     1141 cctggctgct ggcgggcgtt accggttccg gtaaaacgga ggtttatctc agtgtactgg
     1201 aaaacgtgct cgctcagggc aaacaggcgc tggtgatggt gccggaaatc ggcctgacac
     1261 cgcaaactat cgcccgtttt cgtgaacggt ttaatgcgcc cgtggaagtt ctgcattccg
     1321 gtctgaacga cagcgagcgt ctttcggcgt ggctgaaagc gaaaaatggc gaggcggcga
     1381 ttgtgatcgg cacccgctcc gcgctgttta cgccgtttaa aaatctcggc gtgattgtca
     1441 ttgacgaaga gcacgacagt tcctacaagc agcaggaagg ctggcgctat catgcccgcg
     1501 acctggcggt gtatcgtgcg catagcgagc aaatcccgat tattcttggc tccgcaacgc
     1561 ccgcgctgga aacgttgtgc aacgtccagc agaaaaaata ccgcctgctg cgcctgacgc
     1621 gccgggcggg gaatgcgcgt ccggcaattc aacacgtgct ggatttaaaa ggtcagaagg
     1681 tgcaggcagg tctggctccg gcgttaatca cccgtatgcg ccagcattta caggccaaca
     1741 atcaggttat cctctttctt aaccgccgtg gctttgcgcc tgcgctactg tgccacgact
     1801 gcggctggat tgccgaatgc ccacgttgcg atcactacta cacgctgcat caggcgcaac
     1861 agcatttgcg ctgtcaccac tgtgacagtc agcgtccggt gccgcgccag tgcccttcct
     1921 gcggttccac gcacctggta cccgtggggc tgggcaccga acagcttgaa cagacgcttg
     1981 cgccgctgtt ccctgatgtg cccatttctc gtatcgaccg cgataccacc agccgcaaag
     2041 gggcgctgga acagcaactg gcggaagtac atcgcggcgg tgcgaggatt ttgatcggca
     2101 cacaaatgct ggcgaaaggt caccactttc cggatgtgac gctggtggcg ttactggatg
     2161 tggacggcgc gctgttttct gccgattttc gctcggcaga gcgtttcgct cagctttaca
     2221 cccaggtcgc cggtcgtgcc gggcgtgcgg gtaaacaggg cgaagtggtg ctgcaaacgc
     2281 accatccgga acatcctctg ttgcaaacgt tgctctataa aggctacgac gcctttgccg
     2341 aacaggcgct ggctgagcga cgcatgatgc agctgccgcc gtggaccagc catgtgattg
     2401 tgcgtgcgga agatcataac aatcagcatg cgccgctgtt cctgcaacaa ctgcgtaatc
     2461 tgatcctctc cagcccgctg gcagacgaca aattgtgggt tctcggtccg gttccggcac
     2521 tggcacctaa acgtggcggt cgctggcgct ggcagatatt gttgcagcac ccttcccgcg
     2581 tgcgcttgca acacatcatt aacggtacgc tggcactcat caatacaata ccggattccc
     2641 gtaaggtgaa atgggtgctg gatgttgatc cgattgaggg ttaaaccgct cacgatgcga
     2701 ggcggatcga aaaattcaat attcatcaca cttttcatga aaattctgta accgttttca
     2761 cgcgctatct gctaaaaatg ttgccgatgt gaagtaaaca tggatgtagt acgcctgacg
     2821 tgccaggcga ggagtgagtg tgaaagcgaa gaagcaggaa actgccgcga ccatgaaaga
     2881 cgttgccctc aaggcaaaag tctctacagc gactgtctcc cgagcattaa tgaatcccga
     2941 taaagtctcc caggccaccc gtaatcgggt tgaaaaagcg gcccgggaag tgggttattt
     3001 accgcagcct atggggcgca acgtcaagcg taatgaatcc cgcaccattc tggtgattgt
     3061 cccggatatc tgcgatccct tctttagcga aattattcgc ggtatcgaag ttacggcggc
     3121 aaatcacgga tatctggtgc tgattggcga ctgtgcgcat caaaatcagc aggaaaaaac
     3181 ctttatcgat ttgatcatca ccaagcaaat tgatggcatg ttgctgctgg gttcaaggct
     3241 gccgtttgat gccagcattg aggaacagcg taatctgccg ccgatggtga tggcgaacga
     3301 attcgcaccg gaactggagc tacctacagt tcatatcgac aatctgaccg ccgcatttga
     3361 tgcagtaaat tatctatatg agcaagggca taaacggatt ggctgtatag ccggtcccga
     3421 agagatgccg ctgtgtcact accgcctgca aggttacgtt caggcgctgc gtcgctgcgg
     3481 cattatggtt gatccgcaat acatcgcccg tggcgacttc accttcgaag ccggaagcaa
     3541 agcgatgcag cagctgcttg atcttccaca accgcctact gctgtcttct gccatagcga
     3601 tgtgatggcg ctcggcgcac tttctcaggc aaaacgccag gggctgaaag tcccggaaga
     3661 cctttccata atcggttttg ataacatcga cctgacgcaa ttttgtgatc cgccgctgac
     3721 aaccatcgcg cagccgcgtt acgaaatcgg tcgggaagct atgctgttat tgcttgatca
     3781 aatgcagggg caacacgttg gcagtggctc tcgtttaatg gactgcgaac ttatcatccg
     3841 gggatcaaca cgcgcgttac cttaaagtaa acctttaaga ctgccgtgac tggtcaaagg
     3901 cccgccgctt aagtaacatg gcgggctgac gaacgaataa atacagcgaa acgatagtgg
     3961 cacaacgaga ttatgtacgc cgcagccaac cggcaccttc gcggcgaaaa aagagcacct
     4021 cacggaaaaa gcaacgaaat ctgcctgcgg tttctcccgc tatggtcgct attgctgccg
     4081 ccgtgcttgt gacctttatc ggtggtctgt acttcattac gcatcacaag aaagaagagt
     4141 ccgagacgct gcaaagccag aaagtgaccg gtaacggact accaccaaaa ccagaagaac
     4201 gctggcgcta cattaaagag ctggaaagtc gccagccggg tgtgcgtgcg cccacagaac
     4261 cttctgccgg tggtgaagtg aaaacgccgg agcaactgac accagaacaa cgtcagcttc
     4321 ttgaacaaat gcaggctgat atgcgccagc agccaacgca gctggttgaa gttccgtgga
     4381 atgaacagac gccggaacag cgtcagcaaa cgctacaacg ccagcgtcag gcgcagcaat
     4441 tagcggagca gcaacgcctg gctcagcagt cgcgtacgac tgaacaaagc tggcagcagc
     4501 agacgcgtac gtcgcaagcc gcgccggtac aggcgcaacc gcgccagtct aaaccggctt
     4561 ctactcagca gccgtaccag gatctgctgc aaacccctgc gcacacgacc gcgcagtcga
     4621 agccgcagca agctgcgccg gttgctcgtg ccgctgacgc gccaaaaccg acggcggaga
     4681 aaaaagacga acgccgctgg atggtgcagt gcggttcgtt cagaggcgcg gaacaggcag
     4741 agacggtacg tgctcagctg gcgttcgaag gatttgactc gaaaatcacc accaacaatg
     4801 gctggaatcg tgtggtcatt ggcccggtga aaggcaaaga gaacgcagac agcaccctca
     4861 atcggttgaa gatggcgggt catacaaact gcattcggct cgccgccggg ggttgaaacc
     4921 ctcaaaatcc cccccatcta taattgcatt atgccccgta cttttgtacg gggtttgtac
     4981 tctgtattcg taaccaaggg gtcagctcgt gacaactata gtaagcgtac gccgtaacgg
     5041 ccatgtggtc atcgctggtg atggtcaggc cacgttgggc aataccgtaa tgaaaggcaa
     5101 cgtgaaaaag gtccgccgtc tgtacaacga caaagtcatc gcgggctttg cgggcggtac
     5161 tgcggatgct tttacgctgt tcgaactgtt tgaacgtaaa ctggaaatgc atcagggcca
     5221 tctggtcaaa gccgccgttg agctggcaaa agactggcgt accgatcgca tgctgcgcaa
     5281 acttgaagca ctgctggcag tcgcggatga aactgcatcg cttatcatca ccggtaacgg
     5341 tgacgtggtg cagccagaaa acgatcttat tgctatcggc tccggcggcc cttacgccca
     5401 ggctgcggcg cgcgcgctgt tagaaaacac tgaacttagc gcccgtgaaa ttgctgaaaa
     5461 ggcgttggat attgcaggcg acatttgcat ctataccaac catttccaca ccatcgaaga
     5521 attaagctac aaagcgtaag gatctcccat gtctgaaatg accccacgcg aaatcgtcag
     5581 cgaactggat aagcacatca tcggccagga caacgccaag cgttctgtgg cgattgctct
     5641 gcgtaaccgc tggcgtcgca tgcagctcaa cgaagagctg cgccatgaag tgaccccgaa
     5701 aaatatcctg atgatcggcc cgaccggtgt cggtaaaact gaaatcgccc gtcgtctggc
     5761 taagctggcg aatgcgccgt tcatcaaagt tgaagcgacc aaattcaccg aagtgggcta
     5821 tgtcggtaag gaagtggatt ctattattcg cgatctgacc gatgccgccg tgaaaatggt
     5881 acgcgtccag gctatcgaga aaaaccgtta tcgcgctgaa gaactggcag aagaacgtat
     5941 tctcgacgtg ctgatcccac ctgctaaaaa caactgggga cagaccgaac agcagcagga
     6001 accgtccgct gctcgtcagg cattccgcaa aaaactgcgt gaaggccagc ttgatgacaa
     6061 agaaatcgag atcgatcttg ccgcagcacc gatgggcgtt gaaattatgg ctcctccagg
     6121 catggaagag atgaccagcc agctgcagtc catgttccag aacctgggcg gccagaagca
     6181 aaaagcgcgt aagctgaaaa tcaaagacgc catgaagctg ctgattgaag aagaagcggc
     6241 gaaactggtg aacccggaag agctgaagca agacgctatc gacgctgttg agcagcacgg
     6301 gatcgtgttt atcgacgaaa tcgacaaaat ctgtaagcgc ggcgagtctt ccggtccgga
     6361 tgtttctcgt gaaggcgttc agcgtgacct gctgccgctg gtagaaggtt gcaccgtttc
     6421 caccaaacac gggatggtca aaactgacca cattctgttt atcgcttctg gcgcgttcca
     6481 gattgcgaaa ccgtctgacc tgatcccgga actgcaaggt cggctgccaa tccgcgttga
     6541 actgcaggca ctgaccacca gcgacttcga gcgtattctg accgagccga atgcctctat
     6601 caccgtgcag tacaaagcac tgatggcgac tgaaggcgta aatatcgagt ttaccgactc
     6661 cggtattaag cgcatcgcgg aagcggcatg gcaggtgaac gaatctaccg aaaacatcgg
     6721 tgctcgtcgt ttacacactg ttctggagcg tttaatggaa gagatttcct acgacgccag
     6781 cgatttaagc ggtcaaaata tcactattga cgcagattat gtgagcaaac atctggatgc
     6841 gttggtggca gatgaagatc tgagccgttt tatcctataa tcgcgttcaa tcattttcat
     6901 cattgtttga tggggctgaa aggccccatt tttattggcg cgtattatga ctgaacaaca
     6961 aattagccga actcaggcgt ggctggaaag tttacgacct aaaaccctcc ccctcgcctt
     7021 tgctgcaatt atcgtcggga cggcgctggc atggtggcaa ggtcacttcg atccgctggt
     7081 cgccctgctg gtactaatta ccgccgggct attacagatc ctttctaacc tcgccaatga
     7141 ttacggcgat gcggtaaaag gcagcgataa acctgaccgc attgggccgc tacgcggcat
     7201 gcaaaaaggg gtcattaccc agcaagagat gaaacgggcg ctcattatta ccgtcgtgct
     7261 catctgtctc tccgggctgg cactggttgc agtggcatgc catacgctgg ccgattttgt
     7321 cggtttcctg attcttggcg ggttgtcgat cattgccgct atcacctaca ccgtgggcaa
     7381 tcgtccttat ggttatatcg gtctgggtga tatttccgta ctggttttct ttggctggtt
     7441 gagcgtcatg gggagctggt atttacaggc tcatacattg attccggcac tgatccttcc
     7501 ggcgaccgcc tgtggcctgc tggcaacggc agtactgaac attaataacc tgcgtgatat
     7561 caatagcgac cgcgaaaatg gcaaaaacac gctggtggtg cgcttaggtg aagtgaacgc
     7621 gcgtcgttat catgcctgcc tgctgatggg ctctctggta tgtctggcgc tgtttaatct
     7681 cttttcgctg catagcctgt ggggctggct gttcctgctg gcggcaccat tactggtgaa
     7741 gcaagcccgt tatgtgatgc gggaaatgga cccggtggcg atgcgaccaa tgctggaacg
     7801 tactgtcaag ggagcgttac tgactaacct gctgtttgtt ttagggatat tcctaagcca
     7861 gtgggcagca taactgacaa atatcaatta acaattgatg attttgccaa cagcctacat
     7921 agcgcgatat actgaaaatt ctcgcagcaa ctgaatgtta agcctatgaa atacgatact
     7981 tccgagcttt gtgacatcta tcaagaagat gttaacgtcg tggaaccgct gttctccaac
     8041 tttggcggac gggcgtcgtt tggcggacaa ataatcacgg taaaatgttt cgaggacaac
     8101 gggttgctgt acgatctgct cgaacagaat ggccgtggtc gtgttcttgt cgtcgatggc
     8161 ggtggttctg ttcgtcgcgc actggtcgat gctgaactgg cgcgtctggc agtacaaaat
     8221 gaatgggaag gtctggttat ttacggcgcg gtgcgtcagg tagatgacct agaagagttg
     8281 gatatcggca tccaggcgat ggcggcaatt ccggttggtg ccgctggcga aggcattggc
     8341 gaaagcgatg tccgcgtcaa ttttggcggt gtaaccttct tctccggcga ccatctttat
     8401 gccgacaata ccgggattat tctttcagaa gatccgctgg atattgaatg ataagaaagg
     8461 caccgcaagg tgcctttttt ctgcgttacc tgttggccta cacagtaaag aaattacgcg
     8521 aaagatgaag cgtaatcaga cctcttccat gcgacccagc agggcctgca gacgttcctg
     8581 ccagccgttc tgctgttctt tcagatggtt gttctcacgc tccagctctt cgcgctgatg
     8641 ctgggcattt tgaacttcct gcgacagtga gttgtttttt tctttcagct cttcgatttc
     8701 catctgcaac agagtgatgg tatcaatcgc ctgctgtact tttgcttcca gtttctcaaa
     8761 cacttctaat gacattgtca tacctctcct gaattgcaag gcgttgatgg ataaaaaatc
     8821 ctcgtcccga ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt
     8881 gtatgaagcc gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgccacact
     8941 tttcatcctt ctcctggtga cataatccac atcaatcgaa aatgttaata aatttgttgc
     9001 gcgaatgatc taacaaacat gcatcatgta caatcagatg gaataaattg cgcgataacg
     9061 ctcattttat gacgaagcac acacatttta agttcgatat ttctcgtttt tgctcgttaa
     9121 cgataagttt acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac
     9181 aaacattaac tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca
     9241 ttgctgaatt cctcggtacc gggttgttga tttttttcgg tgtgggttgc gttgcagcac
     9301 taaaagtcgc tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg
     9361 tggcaatggc catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta
     9421 ccattgcatt gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt
     9481 cacaagttgc cggcgctttc tgtgctgcgg ctttagttta cgggctttac tacaatttat
     9541 ttttcgactt cgagcagact catcacattg ttcgcggcag tgttgaaagt gttgatctgg
     9601 caggcacttt ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg
     9661 agatggtgat taccgctatt ctgatggggc tgatcctggc gttaacggac gatggcaacg
     9721 gtgtaccacg cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg
     9781 catctatggg cccattgacg ggttttgcca tgaacccagc gcgtgacttc ggtccgaaag
     9841 tctttgcctg gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt
     9901 acttcctggt gccgcttttt ggccctatcg ttggcgcgat tgtaggtgca tttgcctacc
     9961 gcaaactgat tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggagacca
    10021 caactccttc agaacaaaaa gcttcgctgt aatatgacta cgggacaatt aaacatgact
    10081 gaaaaaaaat atatcgttgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtaatg
    10141 gatcacgatg ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa
    10201 ccaggctggg tagaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta
    10261 gaagtgctgg cgaaagccga tatcagttcc gatcaaattg cagctatcgg tattacgaac
    10321 cagcgtgaaa ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt
    10381 gtctggcagt gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa
    10441 gattatatcc gcagcaatac cggtctggtg attgacccgt acttttctgg caccaaagtg
    10501 aagtggatcc tcgaccatgt ggaaggctct cgcgagcgtg cgcgtcgtgg tgaattgctg
    10561 tttggtacgg ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc
    10621 gattacacca acgcctctcg taccatgttg ttcaacatcc ataccctgga ctgggacgac
    10681 aaaatgctgg aagtgctgga tattccgcgc gagatgctgc cagaagtgcg tcgttcttcc
    10741 gaagtgtacg gtcagactaa cattggcggc aaaggcggca cgcgtattcc aatctccggg
    10801 atcgccggtg accagcaggc cgcgctgttt ggtcagttgt gcgtgaaaga agggatggcg
    10861 aagaacacct atggcactgg ctgctttatg ctgatgaaca ccggcgagaa agcggtgaaa
    10921 tcagaaaacg gcctgctgac caccatcgcc tgcggcccga ctggcgaagt gaactatgcg
    10981 ctggaaggtg cggtgtttat ggcaggcgca tccattcagt ggctgcgcga tgagatgaag
    11041 ttgattaacg acgcctacga ttccgaatat ttcgccacca aagtgcaaaa caccaatggt
    11101 gtgtatgtgg ttccggcctt taccgggctg ggtgcgccgt actgggaccc gtatgcgcgc
    11161 ggggcgattt tcggtctgac tcgtggggtg aacgctaacc acattatacg cgcgacgctg
    11221 gagtctattg cttatcagac gcgtgacgtg ctggaagcga tgcaggccga ctctggtatc
    11281 cgtctgcacg ccctgcgcgt ggatggtggc gcagtagcaa acaatttcct gatgcagttc
    11341 cagtccgata ttctcggcac ccgcgttgag cgcccggaag ttcgcgaagt gactgcattg
    11401 ggcgcggcct atcttgctgg tctggcggtc ggtttctggc agaacctcga cgagctgcaa
    11461 gagaaagcgg tgattgagcg cgagttccgt ccaggcatcg aaaccactga gcgtaattac
    11521 cgttacgcag gctggaaaaa agcggttaaa cgcgcgatgg cgtgggaaga acacgacgta
    11581 atgtaaatgc cggatgaagc gtttatgccg catccggtag tcccgaaacg tgcgggggca
    11641 accccgcaca catcaataat ccctcccttc ccctgtgcta cacttcgcgc cattccttac
    11701 tgcttagagt ttgctatgag acgagaactt gccatcgaat tttcccgcgt caccgaatct
    11761 gcggcgctgg ctggctacaa atggttagga cgcggcgata aaaacaccgc ggacggcgcg
    11821 gcggtaaacg ccatgcgtat tatgctcaac caggtcaata ttgacggcac catcgtcatt
    11881 ggtgaaggtg aaatcgacga agcaccgatg ctctacattg gtgaaaaagt cggtactggt
    11941 cgcggcgacg cggtagatat tgctgttgat ccgattgaag gcacgcgcat gacggcgatg
    12001 ggccaggcta acgcgctggc ggtgctggca gtaggcgata aaggctgctt cctcaatgcg
    12061 ccggatatgt atatggagaa gctgatcgtc ggaccgggag ccaaaggcac cattgatttg
    12121 aacctgccgc tggcggataa cctgcgcaat gttgcggcgg cgctcggcaa accgttgagc
    12181 gaactgacgg taacaattct ggctaaacca cgccacgatg ccgttatcgc tgaaatgcaa
    12241 caactcggcg tacgcgtatt tgctattccg gatggcgacg ttgcggcctc aattctcacc
    12301 tgtatgccag acagcgaagt tgacgtgctg tacggtattg gtggcgcgcc ggaaggcgta
    12361 gtttctgcgg cggtgatccg cgcattagat ggcgacatga acggtcgtct gctggcgcgt
    12421 catgacgtca aaggcgacaa cgaagagaat cgtcgcattg gcgagcagga gctggcacgc
    12481 tgcaaagcga tgggcatcga agccggtaaa gtattgcgcc tgggcgatat ggcgcgcagc
    12541 gataacgtca tcttctctgc caccggtatt accaaaggcg atctgctgga aggcattagc
    12601 cgcaaaggca atatcgcgac taccgaaacg ctgctgatcc gcggcaagtc acgcaccatt
    12661 cgccgcattc agtctatcca ctatctggat cgcaaggacc cggaaatgca ggtgcacatc
    12721 ctctgattga tttgatcgat tgagccttcc agtccttcgg gactggaatt tttttgttcg
    12781 gagaacgaag ataaggcaag tcaatcaaaa caggagaaaa acatggctga ttgggtaaca
    12841 ggcaaagtca ctaaagtgca gaactggacc gacgccctgt ttagtctcac cgttcacgcc
    12901 cccgtgcttc cgtttaccgc cgggcaattc accaagcttg gccttgaaat cgacggcgaa
    12961 cgcgtccagc gcgcctactc ctatgttaac tcgcccgata atcccgatct ggagttttac
    13021 ctggtcaccg tccccgatgg caaattaagc ccacgactgg cggcactgaa accaggcgat
    13081 gaagtgcagg tggttagcga agcagctggc ttctttgttc tggaggaagt accagattgc
    13141 gaaacgctat ggatgctggc aaccggtaca gcgattggcc cttatttatc gattctgcaa
    13201 ctaggcaaag atttagatcg cttcaaaaat ctggtcctgg ttcacgccgc acgttatgcc
    13261 gccgacttaa gctatttgcc actgatgcaa gaactggaaa aacgctacga aggcaaactg
    13321 cgcattcaga cggtggtcag tcgggaaacg gcagcggggt cgctcaccgg acggataccg
    13381 gcattaattg aaagtgggga actggaaagc gcgattggcc tgccgatgaa taaagaaacc
    13441 agccatgtga tgctgtgcgg caatccacag atggtgcgcg atacacaaca gttgctgaaa
    13501 gagacccggc agatgacgaa acatttacgt cgccgaccgg gccatatgac agcggagcat
    13561 tactggtaag cggttactta tcgataaacg gcacgatgag caaatccgca ctcatcttat
    13621 tgatcatccc gcgatatgcc ggcatcaaac ggttgataaa tgagtggtga tgaccacaga
    13681 caaggaggtc gcactgctct ttttgcataa tttccagcag tgtttccggc atttctccgc
    13741 gttcaatacg cagttttgtc ttcggccatt gaatattttt cgtcagttta tacagcttgt
    13801 tatccgactt attcttcaac aattgaagaa tatcttctgt tgcagggaag tagatacccg
    13861 gatacaactc gcttaagcca tcatcaatat gaattaacgt caggtgagcg tcattatgtc
    13921 tggcgagctc cagggcttta ttcaccagta aggcatcttc ttcattcccg gaaattgcca
    13981 cgccaatgtg tttataagcc atgtttaact ccttctaaag caactccatc aagctaacga
    14041 acgcagtgat aactcaaaaa taatcatctc ggcctggcat gagaaagtga aggccgcatc
    14101 aagctcaact tgcccctcca gatccttaat cttataggcc acgctggccc cttcatctgc
    14161 agaggatgca gcggcggcaa gctctttcag tttggtcata aaagactctg cttcttcgcg
    14221 ggtggcaaaa aagcgcgaaa acttactggt acagttgtcg ttatcaatca ccgtaccgat
    14281 atcaatcgca catcctttag tactgcattt atctacgaca tctttcatag ggcacctctg
    14341 tatattcgcc tctctgtcga ttacgactta agaataatgc gccgttaagg gaagaacaaa
    14401 caagtaatga tcttatggat taccattttt tttgatattg cttaaggtga agagttcaga
    14461 aggattttcc gggtattgtc atttgcatcg tggaaataac aatcccggaa aaaaatagca
    14521 gtaagagata tattaaaggt taattacttg atttattgtc ggctttatac ttcacatcct
    14581 gagtatcttt accatattta ttttcgcctt gtgtgccaac aaacgcacca agatcgataa
    14641 gcatcatcac cagaatcaac gtcgggacaa aacgccccac cgcccattgc cagacacccg
    14701 gtaaaatcgc ccagttaccc gccagcagca tccacgccac aatcatcaga aatgcccatg
    14761 cgccggaacg cccgcgatca tgcaagcgct taacagttac tgccgctgtt ggccaaagca
    14821 agcacacaag gcaaaacgcc gcggtctgaa tatcgagtaa atttttaccc gccagtgaga
    14881 aaagcaccag catgcctgcg aaccacaggc ctatccaaat ccagaaatca cggcgtccaa
    14941 tacgcccttt aaatgagaat aaccattgct gtatggtcat gtaagttcct tgatggttgt
    15001 cttttccagg attctaccct tttgacaagg gcgacagata tcgttttaat cggagccagt
    15061 cataacaaaa ggtactgtca atgaagccag ggtgtacgct gttttttctc ttatgttctg
    15121 cattaaccgt tacaacaacg gcgcatgcac aaacaccaga tacggcaacg accgcgcctt
    15181 atctgctggc tggagcccct actttcgatc tctccatcag ccagtttcga gaagacttta
    15241 acagccagaa tcctagcctg ccactgaacg aatttcgtgc catcgacagc agtcccgaca
    15301 aagccaatct cactcgtgct gcaagtaaaa ttaacgagaa cttgtatgct tctacagcgc
    15361 tggagcgcgg taccttaaaa atcaaaagca ttcaaatgac ctggctaccc atccaggggc
    15421 cagagcaaaa agcagcgaaa gcgaaagctc aggaatacat ggcagcagtg atccgcacac
    15481 tcaccccatt aatgaccaaa acacaaagcc agaaaaaact gcagtcgcta ctaacggcgg
    15541 ggaaaaacaa acgttattac accgagacag aaggtgcact gcgttatgtt gtcgcggaca
    15601 acagcgaaaa ggggctgacc ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc
    15661 ttgaaggttt gaataaatga caaaaagcaa agcctttgtg ccgatgaatc tctatactgt
    15721 ttcacagacc tgctgccctg cggggcggcc atcttccttt attcgcttat aagcgtggag
    15781 aattaaaatg cgacatcctt tagtgatggg taactggaaa ctgaacggca gccgccacat
    15841 ggttcacgag ctggtttcta acctgcgtaa agagctggca ggtgttgctg gctgtgcggt
    15901 tgcaatcgca ccaccggaaa tgtacatcga tatggcgaag cgcgaagctg aaggcagcca
    15961 catcatgctg ggtgcgcaaa acgtggacct gaacctgtcc ggcgcattca ccggtgaaac
    16021 ttctgccgct atgctgaaag acatcggcgc tcagtacatc atcatcggcc actctgaacg
    16081 tcgtacttac cacaaagagt ctgacgaact gatcgcgaaa aaattcgcgg tgctgaaaga
    16141 gcagggcctg actccggttc tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggcaa
    16201 aactgaagaa gtttgcgcac gtcagatcga cgcggtactg aaaactcagg gtgctgcggc
    16261 attcgaaggt gcggttatcg cttacgaacc tgtatgggca atcggtactg gcaaatctgc
    16321 aactccggct caggcacagg ctgttcacaa attcatccgt gaccacatcg ctaaagttga
    16381 cgctaacatc gctgaacaag tgatcattca gtacggcggc tctgtaaacg cgtctaacgc
    16441 tgcagaactg tttgctcagc cagatatcga cggcgcgctg gttggcggtg cttctctgaa
    16501 agctgacgct ttcgcagtga tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg
    16561 acaggtgccg gatttcatat ccggcactta ctttccttaa ctcttcgcct taacgcaaaa
    16621 tctcacactg atgatcctga atttcctcgg ctgaagcacg gttaagcgtc agtagatttc
    16681 gttgtgtcgc cagcaataca aatgagttat cactctgccg taccatcgcc agcccgtagc
    16741 ttcccatatg ttcccgcgcc tcaggtactt cttctgccag catcataaat gggctgcgtt
    16801 gtaccagttc gctttccgtt acccgacgcg ccaggtattc atgcccacgc aaaccacctg
    16861 gcagtggcaa ccagcggctg ctgatgttcg ccagattgtt atccagttgt tcgcgcacat
    16921 caggacgaat acaagagata tgaatatgaa aatggttttg cgtacgcccg gtgcgggagt
    16981 tgatcgccaa agaaaccgcg cgatcgggaa ccggctggcc gtattttttg ctcataaaat
    17041 cacgcgcctg ccaggccaac caaaagaagt tcggcgttga aggatcggtc aacaaaggac
    17101 tttcagtacc gttaatacga tacgttggca tcaacagata ttgcagtgga ccattaagat
    17161 cttttaaaac cacgtatccg gcattgggtt tgacttccgc acatggcgaa ggattttgat
    17221 tttgctgctg attgggcaaa cattcctcaa ggacaatctt acgtaatgta tccgactctt
    17281 caccggttaa tttccagtaa ccaataccgg cagccacaac ggcgataact atcatcacca
    17341 aaaaaagaag acccgctttt ttcatctttt tttccctgta cctcaaagag atgcaagggt
    17401 aacgcaaaat cgtgacaaat aaaaaacccg gtcgcgagaa accaccgggt ttgataatta
    17461 tcctggggaa atcagcgttt gctgatctga tcgaacgtac cgccgttagc aaaatgctct
    17521 ttttgcgctt tcgtccagcc gccgaactct tcatcaatgg tgaataactt cagctttgga
    17581 aacgcatttt cgtacttttt cgctacttca gcgtcgcgcg gacggtagta gtttttcgcg
    17641 gcaatttcct gaccttctgg cgagtagaga tatttcaggt aggcttccgc cacctctttg
    17701 gtgccttttt tctcgaccac tttatcgacc accgacacgg ttggctctgc gaggatagac
    17761 tcactcggcg tgacgatttc gaatttatct ttccccagtt cattcgctgc cagcagagct
    17821 tcgttttccc aggcgatcag tacatcgcca atcccgcgct cgacaaaggt gttagtggag
    17881 ccgcgcgcgc cagaatccag aacttcgacg tttttataca gtgcccgaac aaaatcctgt
    17941 gcttttgcct gatcgttgtt gttgtgatgc agcgcgtagc cccaggcagc taggtagttc
    18001 cagcgtgcgc caccagagct tttcggatta ggcgtgatca ccgaaacacc cggtttaatc
    18061 agatcgttcc agtcatggat ctgcttcgga ttgcccttac gcaccaggaa aacaatggtg
    18121 gaagtgtacg gtgcggagtt atccggcaga cgtttgatcc actctttatc aatccgccca
    18181 cgttccgcaa tcgcgtccac gtcataggcc agagccagcg tgacaacatc agcttcaata
    18241 ccgttgatta ccgacgtcgc ttgtttacct gaaccaccgt gtgactgacg aatcaccacg
    18301 ttatcaccag tttgctgttt ccagtgggcg ctgaatgcct tgttgtactg ttcgtacaat
    18361 tcgcgcgttg gatcatatga aacgttaaga agctgaatat cctttgccat aacgctggtt
    18421 gccgccagca aaaatgttaa ccctacgccc cacttgttca tcgcccgact ctcttatgtt
    18481 gtgttgtgat gagcaaagcg tgccagaagg ttaaccaaac attaaagaat aaaaaaagat
    18541 tggctataac ttgcgggtat atgttgaggg attaaaaagg cggaagaaat ccgcctcata
    18601 ttgctgacaa agtgcgcttt gtccatgccg gatgcggcgc gaacgcctta tccggcctac
    18661 aaaagtttgc aaattcaata aattgcagaa ttcatgtagg cctgataagc gaagcgcatc
    18721 aggcattttt gcttctgtca tcggtttcag gctaaaggaa tctgcctttt tccgaaatca
    18781 ttaatacagt tttttcgcgc agtccagcca gtcacctttg aacggacgct tcatgttttc
    18841 gatagcgtcg atgatgtcgt ggtgaaccag ctgttcgttc tggataccta cacaacgacc
    18901 gccgtaacct gccagcagca gatcgatagc gtaagcgccc atacgggaag ccagaatacg
    18961 gtcgtaaggc accggagaac caccgcgctg gatgtggccc agcacagttg cgcgggtttc
    19021 acgaccggtt tctttctcga tgaaatgcgc cagttcgtca acatcacaca tatgttcggt
    19081 aatcgccacg atcgcgtgtt ttttaccttt cgcgataccc gctttgattt cgtttaccag
    19141 gtcttcacgg ctgaattcaa cttccggaac cacaacgaat tcacagccac cggcaatggc
    19201 cgcagccaac gtcagatcgc cacaataacg gcccatcact tccaccacgg aaatacgctg
    19261 gtgagaagaa gaggtgtcac gcagacggtc gatcgcttct acaacggtgc tcagcgcagt
    19321 gaagaaaccg atagtgtagt cagtgccttt gatgtcgttg tcgatagtgc ccggcagacc
    19381 gatgcacggg aagcccattt cggtcagacg cattgcaccc atgtaggaac cgtcaccgcc
    19441 gataaccacc agcgcgtcga taccacgttt tttcaggttt tcgatagcca cggcgcggat
    19501 gttctcgtcg cggaattccg ggaagcgcgc agaaccgagg aacgtaccgc cacggttgat
    19561 catgtcagaa acgctgtaac ggtctagctg taccatacgg tcttcataca gacccagata
    19621 gccgtcataa atgcccatta cttccagacc ttctgtcagc gcagaacgaa caaccccgcg
    19681 aattgcggcg ttcatgcctg gcgcatcacc gccgcttgtc aacacaccga ttttcttaat
    19741 catgactacc tctgaacttt ggaatgcaaa ataaaatctg ttgccggaag tcttcttgca
    19801 catcgaagtg atccaacgaa tgtgcaaata gtataacaat cacttcctgc tgaattgatt
    19861 caggtcaggc caaatggcgg tattttatac acaaaatgcg ggtctggctc tcttttatac
    19921 tgattatgaa agcatagacc gtttaccctc cctgggtacg acggaacagg ggtcctgatg
    19981 gataattaca tccgatcccg gaaaacgccg taaaatagcc tgctctacct gatccgccac
    20041 catatgtgcc tgaaccaaag gcagagagtc ttccatttcc aaatgaatct gaataaagcg
    20101 ggtcggccct gactgccgcg tgcgaagatc gtgagcgccg ctaacacccg gccaggaagt
    20161 cacgatatca ataatttctt gccgttcctc atcaggcaat gcgcgatcca gtaatgactg
    20221 taccgcctca tatcccatgc gtaacgcgct atataaaata tagatgccga ttcccaatgc
    20281 aaacagagca tcggcgcgat gccagccgta ccaggacaac cccagcgcca gcagaattgc
    20341 gccgttcatc ataacatcag actggtaatg tagcatatca gcccgcaccg cctggctttg
    20401 cgtccggcgc accacccacc gctgaaacga gacaaggata atcgtacaaa ttagcgccac
    20461 aattgtcacg ataaccccga cgcctggatc tgtcatcggt gttggagata ccagatgttg
    20521 aatacccgtc aaaaacagga atagtgccga accggagata aacatacttt gcgccagcgc
    20581 cgcgagggac tcagctttac cgtgaccaaa cgagtgatta tcgtcggcag gttgcaggga
    20641 atatcgcacc accagtaaat tcgtcaacga cgcgccgata tccaccagcg aatccaccag
    20701 cgcggcgaga atactcaccg acccggtata ccaccatgca aaaattttaa tcagcaatag
    20761 cagcgaagcc atcgccgtcg cagcaatcgc cgcccgactg accagccgtc cataagattg
    20821 attcataaat actcccacta tcaactgacg ctagtataac ggaagcaaat catctgcaat
    20881 gcattaagca gcaggcaaat tgaggataaa aaaaacccca catcatgtgg gggaagacag
    20941 ggatggtgtc tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact
    21001 caatagcttc aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact gctccattcg
    21061 ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc gttaacaggc gatacatttg
    21121 gttgcggact tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc
    21181 ctgtgcgcgc acagcgtttt catcaaaatt ttctgcggtg acaaggcgat gcattgtctc
    21241 cagttcgcta acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat
    21301 ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg
    21361 ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct
    21421 taatgaactg actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg
    21481 ctcccaaaat ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca
    21541 tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt
    21601 tctgcctcgg aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc
    21661 tgacttccct attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg
    21721 atggggaaca ggcgcttgat cttctggacg acagcattga tttacttttg cttgacgtaa
    21781 tgatgccgaa gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc
    21841 ctgtcattat gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg
    21901 gcgcagatga ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg
    21961 cgatcctgcg ccgttcgcac tggagcgagc aacagcaaaa caacgacaac ggttcaccga
    22021 cactggaagt tgatgcctta gtgctgaatc caggccgtca ggaagccagc ttcgacgggc
    22081 aaacgctgga gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg
    22141 gtcaggtggt ttcccgtgaa catttaagcc aggaagtgtt gggcaaacgc ctgacgcctt
    22201 tcgaccgcgc tattgatatg cacatttcca acctgcgtcg taaactgccg gatcgtaaag
    22261 atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta tctgatggtt tctgcttcat
    22321 gataggcagc ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt
    22381 gatgttggtt ttgatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag
    22441 cgaacagcgt caggggctga tgattgagca gcatgtcgaa gcggagctgg cgaacgatcc
    22501 gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc
    22561 aggacagcgt ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga
    22621 aatgcagatc attcgtaact ttattggtca ggccgataac gccgatcatc cgcagaagaa
    22681 aaagtatggc cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg aagataatta
    22741 ccaactttat ctgattcgtc cggccagcag ttctcaatcc gatttcatta acttactgtt
    22801 tgaccgcccg ctattactgc tgattgtcac catgttggtc agtacgccgc tgctgttgtg
    22861 gttggcctgg agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc
    22921 ccagggaaac ttacgccagc acccggaact ggaagcgggg ccacaggaat tccttgccgc
    22981 aggtgccagt tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcagcg
    23041 tctgctttct gatatctctc acgagctgcg caccccgctg acgcgtctgc aactgggtac
    23101 ggcgttactg cgccgtcgta gcggtgaaag caaggaactc gagcgtattg aaaccgaagc
    23161 gcaacgtctg gacagcatga tcaacgatct gttggtgatg tcacgtaatc agcaaaaaaa
    23221 cgcgctggtt agcgaaacca tcaaagccaa ccagttgtgg agtgaagtgc tggataacgc
    23281 ggcgttcgaa gccgagcaaa tgggcaagtc gttgacagtt aacttcccgc ctgggccgtg
    23341 gccgctgtac ggcaatccga acgccctgga aagtgcgctg gaaaacattg ttcgtaatgc
    23401 tctgcgttat tcccatacga agattgaagt gggctttgcg gtagataaag acggtatcac
    23461 cattacggtg gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac agattttccg
    23521 tccgttctat cggaccgatg aagcacgcga tcgtgaatct ggcggtacag gattggggct
    23581 ggcgattgtt gaaaccgcca ttcagcagca tcgtggctgg gtgaaggcag aagacagccc
    23641 gctgggcggt ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaactccgca
    23701 tttgtaggca ggataaggcg tttacgccgc atccggcatt tgagcaggat gcctgatgcg
    23761 acgctgatag cgtcttatca ggcctacact ccgggggtta ttttccccac aaccggcggg
    23821 aaaaatcttc gatcttgccg ctcagtcggc gcttttgcat cgtccgcgtc cagcttttcg
    23881 ataacccggc ggcggagagt aaacggtgat actgatcgtc atcaaacggc atatgccatg
    23941 cgatggcggc agcctcctgc acggtcacat cgctgacacg ggaaaccaac tccagcggcg
    24001 cgtccgcaga cactttccct ggcgctatca cgctatacaa ccaccccacc ttacctgtgt
    24061 tttgcatcaa ctgcgctata tcgctgatat caaaatggta attgagttta tagcaaggcg
    24121 agcgcggctg gctgacctga attaatgcct ctccccagcg gaaaatatcg cccatatgaa
    24181 cattactttc cgtgagaccg tcggttgaga gattttcgcc aaacgcaggc gcaacaaaca
    24241 actccgcctg ttccggaaat tcccgcaccc agtagagata gtgctcacga ggataatgac
    24301 acagcgctct gtctggcccg ccgtgaacct ttttctccgc ctgctcgtca ccttccagcc
    24361 ccagctccgt cagcatcaac tcaccatcaa cctggatttt ggcaattgcg ctgggtttgc
    24421 cttcgggata agcctgaatc ttgcctgtgt atacatcaac cggatatcgc atctgttccc
    24481 tctccacgag aaatttcccc attagaccat atttatttta tgtcaggcgt gctgttttcg
    24541 cggtaagtat ccaggcagga actcaatagc aaaccggacg aacgttagtt catccggctg
    24601 tggggattaa ggaagcaggt ttatttcacc gtacctaaaa tttcgatttt cgctgctctg
    24661 gctttgactt tacgtgacca gatagaggta agaattggca ccagaatcga agtcacaatg
    24721 accgccgtcg ctaccagcga agttgctgcc ggagccatcg gtttaaacgc aggcaccatt
    24781 tctgcaatca gcacaggtgt cgctaccgcg gcccctgcgg aactggaagc ggcaattccg
    24841 gcagtgccat cgccaccgcc aatcaattta tcggcgataa tcaacggaat accggtcacg
    24901 ataattactg ccacacccaa caggatcccc agcaaaccag tctgggcaat cacagtcaaa
    24961 tcaatggtat tgcccagcgc gaaggcaaag aatggaatca gcgtttgcac tgctttgctg
    25021 aaaaattctc gtaattcagg gtcaagattc cccagggcaa agcccaccag gaacggtaat
    25081 actgcgccga cgaaaacatg cggttcaaac gaggcaatcc cggcagtgcc cagaataatc
    25141 atcgtcatga gcggcccgga ctccaacgac atcaacacaa atgccccagc ttcttctttc
    25201 gtaccgtact gctgcatgat ggaagcgtaa agtccgccgt tggtcatatc catcgccgcc
    25261 accagcgcca gcgttgaaag tccggcaaag aatccaactt caacaccatg ttccggaata
    25321 atgcgcgagg caattgccgc aaccacccac gcgacggcaa ttttagttac caccagcgta
    25381 ccggatttac gcagtaccgt tcccgtcgcg cttaatttta ttgacgcccc catgcaaaaa
    25441 aaccacaccg ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa tgatccaaaa
    25501 tatttccccg cccccggcga gaaggtgtgg cacagtgcgc caaggaatag cgggacgagc
    25561 atcatccccc ccgggatttt ctcaatcgag cgttttatct gcatttccat cacctgtcgg
    25621 catattttta agggctgatg cgtaattcct caatggagca tcagtgttga tggaaacgaa
    25681 gatagacatt catttaatca ataaaagtga tcaaaaccac atcaattgaa acgctgtttt
    25741 atttttatcg gatcattgtt tttattttga aattgatcac aaaaaaacca ccgccgttgg
    25801 cgatggttca ttttttaagc tgatatgcgg cctatacgcc tcattgcagc aggcggcaaa
    25861 tgattatttt ttcgccgcaa aacgtgccgc tgcttcgtcc cagttcacca cgttccagaa
    25921 ctctttaatg tagtccggac ggcggttctg gaatttcagg tagtaagcat gttcccacac
    25981 atccaggccc agaatcggga agccggaagc gccagaaata gcttcaccca tcagcggaga
    26041 atcctggtta gcagtagaaa ccaccgccag tttatcgcct ttcagcacca gccatgccca
    26101 gccggaacca aagcgggaag ctgccgcttt ttcaaattct gctttgaagt tatcaacgga
    26161 gccgaagtca cgttcgatag ccgctttcag gtcaccctgc agggtggtgc cttttttcag
    26221 acctttccag aacaggctgt ggttagcgtg accgccagcg ttgttgcgca gtacggtttt
    26281 cttgtctgct ggcagctggt ccagtttggt gatcagctct tcaaccggca ggttggcaaa
    26341 ttctggcagg ctttccagcg ccgcgttggc gttgtttacg taggtctgat ggtgtttggt
    26401 gtggtggatt tccatggtct gcttatcgaa gtgcggttcc agggcatcgt aagcatacgg
    26461 cagggatggc agggtatagc tcatattcat ctccagtatt gtcgggcggc cgattgttaa
    26521 tgccgcgtaa gcagttggtt cattatagtt aattaaatga tattgaaaat gattatcaat
    26581 gccgtacttt tcgtaagggt atggttttgc aggaaaatgc ccgagatgtg aagcaaatca
    26641 cccacttaat gccgtgattg ccagtaaatc gacaacggcg gcaacaggcg aaaggttaat
    26701 cgacagcacg atttttacac tcatctcgtc ggagatgtga agcgacgaaa aatgatgagg
    26761 ataagaagat gagtaacgcg attacgatgg ggatattttg gcatttgatc ggcgcggcca
    26821 gtgcagcctg tttttacgct ccgttcaaaa aagtaaaaaa atggtcatgg gaaaccatgt
    26881 ggtcagtcgg tgggattgtt tcgtggatta ttctgccgtg gaccatcagc gccctgttac
    26941 taccggattt ctgggcgtat tacagctcgt ttagtctctc tacgctgctg cctgtttttc
    27001 tgttcggcgc tatgtggggg atcggtaata tcaactatgg cctgaccatg cgttatctcg
    27061 gcatgtcgat gggaattggc atcgccattg gcattacgtt gattgtcggt acgctgatga
    27121 cgccaattat caacggcaat ttcgatgtgc tgattaacac cgaaggcgga cgcatgacgt
    27181 tgctcggcgt tctggtggcg ctgattggcg cagggattgt aactcgagcc gggcagttga
    27241 aagagcgcaa gatgggcatt aaagccgaag agttcaatct gaaaaaaggg ctggtgctgg
    27301 cggtgatgtg cggcattttc tctgccggga tgtcctttgc gatgaacgcc gcaaaaccga
    27361 tgcatgaagc cgctgccgca cttggcgtcg atccactgta tgtcgctctg ccaagctatg
    27421 ttgtcatcat gggcggcggc gcgatcatta acctcggttt ctgttttatt cgtctggcaa
    27481 aagtgaagga tttgtcgcta aaagccgact tctcgctggc aaaaccgctg atcattcaca
    27541 atgtgttact ctcggcactg ggcgggttga tgtggtatct gcaattcttt ttctatgcct
    27601 ggggccacgc ccgcattccg gcgcagtatg actacatcag ttggatgctg catatgagtt
    27661 tctatgtatt gtgcggcggt atcgtcgggc tggtgctgaa agagtggaac aatacaggac
    27721 gccgtccggt aacggtgttg agcctcggtt gtgtggtgat tattgtcgcc gctaacatcg
    27781 tcggcatcgg catggcgaat taatctttct gcgaattgag atgacgccac tggctgggcg
    27841 tcatcccggt ttcccgggta aacaccaccg aaaaatagtt actatcttca aagccacatt
    27901 cggtcgaaat atcactgatt aacaggcggc tatgctggag aagatattgc gcatgacaca
    27961 ctctgacctg tcgcagatat tgattgatgg tcattccagt ctgctggcga aattgctgac
    28021 gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt atccagcgca aagggacttt
    28081 tcaggctagc cgccaaccgg gtaatcagct tatccagcaa cgtttcgctg gatgttggcg
    28141 gcaacgaatc actggtgtaa cgatggcgat tcagcaacat caccaactgc ccgaacagca
    28201 actcagccat ttcgttagca aacgacacat gctgactact ttcatgctca agctgaccga
    28261 taacctgccg cgcctgcgcc atccccacgc tacctaagcg ccagtgtggt tgccctgcgc
    28321 tggcgctaaa tcccggaatc gctccctgtc agtcaagatt cagcttcagc cgctccgggc
    28381 aataaataat attctgcaaa accagatcgt taacggaagc gtaggagtgt ttatcgtcag
    28441 catgaatata aaagagatcg ccacgggtaa tgcgataagg acgatcgttg agtacatgca
    28501 gaccattacc gcgccagaca atcaccagct cacaaaaatc atgtgtatgt tcggcaaaga
    28561 catcttgcgg ataacggtca gccacagcga ctgcctgctg gtcgctggca aaaaaatcat
    28621 ctttgagaag ttttaactga tgcgccaccg tggctacctc ggccagagaa cgaagttgat
    28681 tattcgcaat atggcgtaca aatacgttga gaagattcgc gttattgcag aaagccatcc
    28741 cgtccctggc gaatatcacg cggtgaccag ttaaactctc ggcgaaaaag cgtcgaaaag
    28801 tggttactgt cgctgaatcc acagcgatag gcgatgtcag taacgctggc ctcgctgtgg
    28861 cgtagcagat gtcgggcttt catcagtcgc aggcggttca ggtatcgctg aggcgtcagt
    28921 cccgtttgct gcttaagctg ccgatgtagc gtacgcagtg aaagagaaaa ttgatccgcc
    28981 acggcatccc aattcacctc atcggcaaaa tggtcctcca gccaggccag aagcaagttg
    29041 agacgtgatg cgctgttttc caggttctcc tgcaaactgc ttttacgcag caagagcagt
    29101 aattgcataa acaagatctc gcgactggcg gtcgagggta aatcattttc cccttcctgc
    29161 tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata cgctgtggtt aacgcgccag
    29221 tgagacggat actgcccatc cagctcttgt ggcagcaact gattcagccc ggcgagaaac
    29281 tgaaatcgat ccggcgagcg atacagcaca ttggtcagac acagattatc ggtatgttca
    29341 tacagatgcc gatcatgagc gcgtacgaaa cagaccgtgc caccggtgat ggtatagggc
    29401 tgcccattaa acacatgaat acccgtgcca tgttcgacaa tcacaatttc atgaaaatca
    29461 tgatgatgtt caggaaaatc cgcctgcggg agccggggtt ctatcgccac ggacgcgtta
    29521 ccagacggaa aaaaatccac actatgtaat acggtcatac tggcctcctg atgtcgtcaa
    29581 cacggcgaaa tagtaatcac gaggtcaggt tcttacctta aattttcgac ggaaaaccac
    29641 gtaaaaaacg tcgatttttc aagatacagc gtgaattttc aggaaatgcg gtgagcatca
    29701 catcaccaca atgaagctaa ttgtgaacat catcacgttc atctttccct ggttgccaat
    29761 ggcccatttt cctgtcagta acgagaaggt cacgaattca ggcgcttttt agactggtcg
    29821 taatgaaatt cagcaggatc acattatgac ctttcgcaat tgtgtcgccg tcgatctcgg
    29881 cgcatccagt gggcgcgtga tgctggcgcg ttacgagcgt gaatgccgca gcctgacgct
    29941 gcgcgaaatc catcgtttta acaatggcct gcatagccag aacggttatg tcacctggaa
    30001 tgtggatagc ctggaaagtg ccattcgcct tggattaaac aaggtgtgcg aggaagggat
    30061 tcgtatcgat agcattggga ttgatacctg gggcgtggac tttgtgctgc tcgaccaaca
    30121 gggtcagcgt gtgggcctgc ccgttgctta tcgcgatagc cgctccaatg gcctaatggc
    30181 gcaggcacag caacaactcg gcaaacgcga tatttatcaa cgtagcggca tccagtttct
    30241 gcccttcaat acgctttatc aactgcgtgc gctgacggag caacaacctg aacttattcc
    30301 acacattgct cacgctctgc tgatgccgga ttacttcagc tatcgcctga ccggcaagat
    30361 gaactgggag tacaccaacg ccacgaccac gcaactggtc aatatcaata gcgacgactg
    30421 ggacgagtcg ctactggcgt ggagcggggc taacaaagcc tggtttggtc gcccgacgca
    30481 tccgggtaat gtcattgggc actggatttg cccgcagggt aatgagattc cggtggtcgc
    30541 cgttgccagc catgataccg ccagcgcggt tatcgcctcg ccgttaaacg gttcacgcgc
    30601 tgcttatctc tcttctggca cctggtcatt gatgggcttc gaaagccaga cgccatttac
    30661 caatgacacg gcgctggcag ccaacataac caatgaaggc ggggcggaag gtcgctatcg
    30721 ggtgctgaaa aatattatgg gcttatggct gcttcagcga gtgcttcagg agcggcaaat
    30781 caacgatctc ccggcgctta tcgccgcgac acaggcactt ccggcctgcc gcttcatcat
    30841 caatcccaat gacgatcgct ttattaatcc tgaggcgatg tgcagcgaaa ttcaggctgc
    30901 gtgtcgggaa atggcgcaac cgctcccgga aagtgatgct gaactggcgc gctgcatttt
    30961 cgacagtctg gcgctgctgt atgccgatgt gttgcatgag ctggcgcagc tacgcggtga
    31021 agatttctcg caactgcata ttgtcggcgg cggctgccag aacacgctgc tcaaccagct
    31081 atgtgccgat gcctgcggta ttcgggtgat cgccgggcct gttgaagcct cgacgctcgg
    31141 caatatcggc atccagttaa tgacgctgga tgaactcaac aatgtggatg atttccgtca
    31201 ggtcgtcagc accaccgcga atctgaccac ctttacccct aatcctgaca gtgaaattgc
    31261 ccactatgtg gcgcagcttc actcgacacg acagacaaag gagctttgcg catgaccact
    31321 caactggaac aggcctggga gctggcgaaa cagcgtttcg cggcggtggg gattgatgtc
    31381 gaggaggcgc tgcgccaact tgatcgttta cccgtttcaa tgcactgctg gcagggcgat
    31441 gatgtttccg gttttgaaaa cccggaaggt tcgctgaccg gtgggattca ggctattggt
    31501 aattatccgg gcaaagcgcg taatgccagt gagctacgag ccgatctgga acaggctatg
    31561 cggctgattc cggggccgaa acggcttaat ttacatgcca tctatctgga atcagacacg
    31621 ccagtctcgc gcgaccagat caaacctgag cacttcaaaa actgggttga atgggcgaaa
    31681 gccaatcagc tcggtctgga ttttaacccc tcctgtttct cgcatccgct aagcgccgat
    31741 ggctttacgc tctcgcatcc cgacgacagc attcgccagt tctggattga tcactgcaaa
    31801 gccagccgtc gcgtttcggc ctattttggc gagcaactcg gcacaccatc ggtgatgaac
    31861 atctggatcc cggatggtat gaaagatatc accgttgacc gtctcgcccc gcgtcagcgt
    31921 ctgctggcag cactggatga ggtgatcagc gagaagctgg atccggcgca ccatatcgac
    31981 gccgttgaga gcaaattgtt tggcattggc gcagagagct acacggttgg ctccaatgag
    32041 ttttacatgg ggtatgccac cagccgccag actgcgctgt gcctggacgc cgggcatttc
    32101 cacccgactg aagtgatttc cgacaagatt tccgccgcca tgctgtatgt accgcagttg
    32161 ctgctgcacg tcagccgtcc ggttcgctgg gacagcgatc acgtggtgct gctggatgat
    32221 gaaacccagg cgattgccag tgagattgtt cgtcacgatc tgttcgaccg ggtgcatatc
    32281 ggcctcgact tctttgatgc ctctatcaac cgcattgccg cgtgggtcat tggtacacgc
    32341 aatatgaaaa aagccctgct gcgtgcgttg ctggaaccta ccgctgagct gcgcaagctg
    32401 gaagcggcgg gcgattacac tgcgcgtctg gcgctactgg aagaacagaa atcgttgccg
    32461 tggcaggcgg tctgggaaat gtattgccaa cgtcacgata cgccagcagg tagcgaatgg
    32521 ctggagagcg tgcgggctta tgagaaagcg attttgagcc agcgtgggta aacactgccg
    32581 gatgcggcgc gagcgcctta tccggcctac aggtcgaatg accatgcgtg ctgccaccct
    32641 caccccggcc ctctccctga aagggcgagg gagaaaaaca tagcctcgtg cggcaacgga
    32701 gctgaacacc gctccggaca gtcccctcgc ccctacgggg agagggttag ggtgagggga
    32761 acaagccgca ccatgtctaa acaaggcaac ctaatttaca ggaacacaga acatgcaaaa
    32821 cattacccag tcctggtttg tccagggaat gatcaaagcc accaccgatg cctggctgaa
    32881 aggctgggac gagcgcaatg gtggtaacct gacgctgcgc ctggatgacg ccgatatcgc
    32941 gccttatcac ggcaactttc atgcacaacc gcgctatatt cccctcagcc aacccatgcc
    33001 tttactggca aatacgccgt ttattgtcac cggctccggc aaattcttcc gtaacgtcca
    33061 gcttgatcct gcagctaact taggcgtcgt aaaagtcgac agcgacggcg cgggctacca
    33121 tattctctgg gggttaacca acgaagccgt tccaacttcc gaacttccgg ctcacttcct
    33181 ttcccactgc gagcgcatta aagccaccaa cggtaaagat cgggtgatca tgcactgcca
    33241 cgccaccaac ctgatcgccc tcacctatgt acttgaaaac gacaccgcgg tctttactcg
    33301 ccaactgtgg gaaggcagca ccgagtgtct ggtggtattc ccggatggcg ttggcattct
    33361 gccgtggatg gtgcccggca cggacgaaat cggccaggcg accgcacaag agatgcaaaa
    33421 acattcgctg gtgttgtggc ccttccacgg cgtcttcggc agcgggtcga cgttggatga
    33481 aacattcggt ttaatcgaca ccgcagaaaa atcagcacaa gtattagtga aggtttattc
    33541 gatgggcggc atgaaacaga ccatcagccg tgaagagttg atagcgctcg gcaagcgctt
    33601 cggcgttaca ccactagcta gtgcgctggc gctgtaagga gcaaacatga tccgcaaagc
    33661 ctttgtcatg caggttaacc ccgacgccca cgaagagtat cagcgtcggc ataaccccat
    33721 ctggccggaa ctggaagcag tgttgaaatc tcacggtgcg cataactacg ccatttacct
    33781 cgacaaagcg cgtaatctgc tgtttgccac ggtagagatt gaatctgaag aacggtggaa
    33841 tgcggtagcc agcactgatg tttgccaacg ttggtggaaa tatatgaccg atgttatgcc
    33901 cgctaacccg gataacagcc cggtgagtag cgagctgcaa gaagtgtttt acctgccgta
    33961 attgcccaat gtgccagaga tgcctccggc acaatatttt aaatttcgat ttgaaaaatt
    34021 aaacatatta catctcccgg taagttatat ttccctgata cattgtgagt aaatcacaaa
    34081 aataatgaat aacccattaa tgattcatgt ggtttattta aataaccatt atgtgcatta
    34141 ctccgcaaat ctgacctttc actctgtctc aaattgtcat tgtttaccga catatcgcct
    34201 ctcatcactt aatcgctcca ccattacaat tgattaaaat atattttatg gagtccgatt
    34261 aatggcagct cttactgcaa gctgtattga cctgaatatt cagggcaatg gcgcttattc
    34321 cgttctgaag cagttggcga caatagcgtt acaaaacggt tttatcaccg actcacacca
    34381 gttcctgcaa accctgctgc tgcgcgaaaa aatgcactct actggatttg gttccggtgt
    34441 cgccgtgccg cacggtaaaa gcgcctgcgt taaacaaccg tttgtattat tcgcccgcaa
    34501 agcgcaggct attgactgga aagccagcga tggcgaagac gtcaattgct ggatctgcct
    34561 cggcgtgccg caaagcggcg aagaggatca ggtcaaaatc atcggcacac tgtgtcgcaa
    34621 aattatccac caggatttta ttcatcaact gcaacagggc gacgccgacc aggtgcttgc
    34681 cttgttaaat caaaccctca gctcataagg aagtggcgat ggagtcatcc ttatgtattg
    34741 tcgcgatcac caactgcccc gccgggatcg ctcacaccta catggtggcg gaagccctgg
    34801 aacagaaagc ccgttctctc ggccatacca taaaagtgga aactcaaggg tccagtggcg
    34861 ttgaaaaccg cttatccagc gaagagattg ccgctgccga ttacgtcatt ctcgctaccg
    34921 ggcgtggcct gagcggtgat gatcgcgcgc ggtttgccgg gaagaaagtt tatgagattg
    34981 ccatctccca ggcgttgaaa aatatcgacc agattttcag cgaattaccg acaaactcgc
    35041 agctttttgc cgcagatagc ggcgtgaagc ttggcaaaca ggaagtgcaa agcggcagcg
    35101 tgatgagcca cctgatggcg ggcgtctctg cggcgctgcc gtttgtcatc ggcggtggta
    35161 tcctggtggc gcttgccaac atgctggtgc agttcggttt gccgtatacc gatatgtcga
    35221 aaggcgcgcc gtcgtttacc tgggtagttg aatccatcgg ttaccttggc ttcaccttta
    35281 tgatccccat catgggcgct tatattgcct cgtcgattgc cgataaaccg gctttcgctc
    35341 cggcgtttct ggtttgctat ctggcgaacg acaaagcgct gctcggcacc cagtcgggtg
    35401 cgggattcct cggcgcggtg gtgctggggc tggcgattgg ctatttcgtc ttctggttcc
    35461 gcaaagtgcg ccttggcaaa gcattacaac cgctgctcgg ttcaatgctg atcccgttcg
    35521 ttactctgct ggttttcggc gtgctcactt actacgttat cgggccggtg atgtccgacc
    35581 tcatgggcgg gctgctccac ttcctgaata ccattcctcc atcaatgaag tttgcggcag
    35641 cgtttctggt cggtgcgatg ctggcgttcg atatgggcgg ccccatcaat aaaaccgcct
    35701 ggttcttctg cttctcactg ctggaaaaac acatttatga ctggtacgcc atcgtcggcg
    35761 ttgtcgcgtt gatgccgccc gttgcggcgg gtcttgcgac gttcattgcg cccaaactgt
    35821 ttacccgaca ggaaaaagag gctgccagta gtgccattgt ggtcggtgcc acagtcgcaa
    35881 ccgaaccggc tataccttac gctctggcag ctccgttgcc gatgattacc gccaatacgc
    35941 tggcgggtgg cattaccggc gtgctggtga tcgctttcgg aatcaaacgt ctggcacctg
    36001 gtttgggtat ctttgacccg ctgatcggcc tgatgtcgcc ggtgggttcg ttttatctgg
    36061 tgctagcgat tggcctcgcg ctgaacattt cattcattat cgtcctgaaa ggattgtggt
    36121 tacgccgcaa agcgaaagcc gcgcaacagg agcttgtcca tgaacattga gttactgcaa
    36181 cagttgtgcg aagccagcgc cgtcagcggc gatgaacagg aagttcgcga cattctgata
    36241 aacacgctgg aaccttgcgt taatgagatc acctttgatg gtctgggcag ctttgttgcc
    36301 cgtaagggga ataaaggtcc aaaagttgcc gttgtcaggc atatggatga agtcggcttt
    36361 atggtcaccc acatcgacga gagcggtttt ctgcgcttta ccaccattgg cggctggtgg
    36421 aatcagtcaa tgctcaacca ccgggtaacc atacgcacac acaagggatt taaaatccct
    36481 ggtgtgattg gttccgtcgc acctcatgcg ttaacggaaa agcaaaagca acagccgctg
    36541 tcatttgatg agatgttcat tgatattggc gcgaacagtc gcgatgaggt ggaaaaacgc
    36601 ggtgtggcga tgggcgattt tattagcccg gaagccaatt ttgcctgctg gggcgaagat
    36661 aaagtggtcg gcaaggcgct ggataaccgc atcggctgcg caatgatggc tgaactattg
    36721 cagacggtga ataatcccga aattacgctg tatggcgtcg gcagcgtgga agaagaagtt
    36781 gggctacgcg gggcacaaac ctcggcggaa cacattaaac cggatgtggt gattgtgctg
    36841 gataccgctg tcgcaggtga tgttccgggc attgataaca ttaaataccc gctgaaactg
    36901 ggccaggggc cggggctgat gctgtttgac aagcgctact tccccaacca gaaactggtg
    36961 gcggcgttaa aaagctgtgc cgcacataac gatttaccgc tgcaattttc caccatgaaa
    37021 accggtgcga cggatggcgg gcgctacaac gtgatgggcg gcgggcgtcc ggttgttgcg
    37081 ctgtgcctgc caacccgcta tctgcacgct aacagcggca tgatttcaaa agccgattac
    37141 gatgctctgc tcacgttgat acgggatttt ctgaccacct taaccgcgga gaaagtcaac
    37201 gcgtttagcc agttccgtca ggtggattaa tgctaaacga acgccagtta aagattgtcg
    37261 atctgctgga gcaacagccg cgcacgcctg gtgagctggc gcaacagact ggcgtttcag
    37321 gcaggaccat cctgcgtgat attgactatc tcaactttac ccttaacggc aaagcccgca
    37381 ttttcgccag tggcagtgcg ggctatcagc tggaaatctt cgagcgccgc agcttttttc
    37441 agttgctgca aaagcacgat aacgacgatc ggctgctggc gctgttatta ctgaatacct
    37501 ttactccccg tgcgcaactc gcctcggcgc ttaatttgcc agaaacctgg gtagcagagc
    37561 gtctgccccg gttaaaacag cgttatgaac gcacttgttg cctggccagt cgccccggtt
    37621 tgggccattt cattgatgag acagaagaga aacgcgttat cttgctggcg aacttgctgc
    37681 gcaaagatcc gtttttaatt ccgctggcgg gcgtaacacg agacaatttt cagcatttat
    37741 ccacggcctg cgacaaccaa caccgctggc cgctcatgca gggtgattat ctctccagcc
    37801 tgattctggc gatttacgcc ctgcgtaatc aactgaccga tgagtggccg caatatcccg
    37861 gtaacgagat aaaacaaatc gttgaacaga gcggcatgtt tcttggtgat aacgctgtaa
    37921 gaaccctgac aggtttgata gagaaacagc atcagcaagc gcaggtaatt tcagccgatc
    37981 atgtgctggg gttgctgcaa agggtgccag gcatcgcgtc attgaatatt attgatacgc
    38041 agctggttga gaatattacc gggcatttat tacgttgcct tgccgcaccg gtgtggattg
    38101 ccgagcaccg ccaaagcagc atgaataacc tgaaagccgc ctggcctgcg gcctttgata
    38161 tgagtctgca ctttattacg ctactgcgtg aacagctcga tattcccctt ttcgatagcg
    38221 atctgatcgg tttgtatttt gcctgtgcgc tggaacggca tcaaaacgaa cgccagccga
    38281 tcattttgct ctcggaccag aacgcgattg ccactattaa tcagctcgcc attgagcgcg
    38341 atgttttaca ttgtcgggtg attattgccc gtagcttaag cgagcttatt gccattcgcg
    38401 aagagattga gccgttattg atcattaaca acagccatta tttactggat gacgcggtaa
    38461 ataattacat caccgtaaaa aatatcatta cggctgccga tatcgaacaa ataaaacact
    38521 ttctggcgac ggcatttatt cgccaacagc cggaacgctt tttctctgcc cccggaagtt
    38581 ttcattattc gaatgtacgc ggtgaaagct ggcaacatat tacccggcaa atttgtgcgc
    38641 aattagtggc acaacaccat attaccgccg atgaagcgca acggatcatc gcccgcgaag
    38701 gcgaaggtga aaacctgatt gttaatcgcc tcgcaatccc acattgctgg agcgaacagg
    38761 agcgacgttt tcgtggattt tttattaccc tcgcccaacc agttgaggtg aataacgaag
    38821 tcattaacca tgtcttgatc gcctgcgccg ccgccgatgc gcgtcatgag ctaaaaatat
    38881 ttagctatct ggcaagcgta ttgtgtcagc atccggcaga agttattgcc gggttaacag
    38941 gatatgaggc atttatggag ttacttcaca aggggtgaac tgtgtcggat gcgccgcatc
    39001 cgacactgca ttttcatcaa aaacgtttaa cgcaggctat tatagtcatc aaccatttcg
    39061 ttgtactcgc gtaacgctcg tggaaatgag tcttcgacca tccagcttgt gtttgcatcc
    39121 tgaagatgct ctttatccca gtcagagtac gggactttat ctctgacgcg gcgaacatat
    39181 tttttagtct cgagttgata ctggtctgcc gaattaatga acgagaaatt cataccgcct
    39241 ttatccgctt ctttggcctg cgctaccagc gtttccagtt cagagacttt cttcattgcc
    39301 gcatca
//
LOCUS       DAJTVJ010000026        39249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000026 DAJTVJ010000000
VERSION     DAJTVJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.026"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO448_001291"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO448_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6336548.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO448_001292"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO448_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6336549.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OO448_001293"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OO448_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6336550.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OO448_001294"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OO448_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6336551.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OO448_001295"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OO448_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6336552.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OO448_001296"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OO448_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6336553.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OO448_001297"
     CDS             complement(6574..7338)
                     /locus_tag="OO448_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6336554.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO448_001298"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO448_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6336555.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OO448_001299"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OO448_001299"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6336556.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OO448_001300"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OO448_001300"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6336557.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OO448_001301"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OO448_001301"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6336558.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OO448_001302"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OO448_001302"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6336559.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OO448_001303"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OO448_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6336560.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OO448_001304"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OO448_001304"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6336561.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO448_001305"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO448_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6336562.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OO448_001306"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OO448_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6336563.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OO448_001307"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OO448_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6336564.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OO448_001308"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OO448_001308"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6336565.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OO448_001309"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OO448_001309"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6336566.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OO448_001310"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OO448_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6336567.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OO448_001311"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OO448_001311"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6336568.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO448_001312"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO448_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6336569.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OO448_001313"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OO448_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6336570.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OO448_001314"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OO448_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6336571.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="OO448_001315"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="OO448_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OO448_001316"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OO448_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6336572.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="OO448_001317"
     CDS             complement(31717..33003)
                     /locus_tag="OO448_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6336573.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="OO448_001318"
     CDS             complement(33054..33995)
                     /locus_tag="OO448_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6336574.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="OO448_001319"
     CDS             complement(34010..34780)
                     /locus_tag="OO448_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6336575.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35254..36768
                     /gene="caiT"
                     /locus_tag="OO448_001320"
     CDS             35254..36768
                     /gene="caiT"
                     /locus_tag="OO448_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6336576.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36799..37941
                     /gene="caiA"
                     /locus_tag="OO448_001321"
     CDS             36799..37941
                     /gene="caiA"
                     /locus_tag="OO448_001321"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6336577.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38070..39249
                     /gene="caiB"
                     /locus_tag="OO448_001322"
                     /pseudo
     CDS             38070..39249
                     /gene="caiB"
                     /locus_tag="OO448_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9393 a        10790 c        10277 g         8789 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaaatg
    35101 tgataacaat cacagaatgc agcttattga atacccatta tgagttagcc attaacgcgt
    35161 ccacgaggtt aataataatt atatcaaatg ttaacaaaaa taaaacaaac gggaacgcaa
    35221 aataaatatt cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga
    35281 atagaaccga aggttttctt tccgccgtta ataatcgtcg gcatactttg ttggcttaca
    35341 gtcagagatc tggatgcagc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat
    35401 gtatggggat gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg
    35461 gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac cacctgaatt tagtaccgcc
    35521 agttggatct ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg
    35581 attgagatct actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacaggg
    35641 gcgaaagagt tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact
    35701 tacagcttcc tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt
    35761 cgccccagct cgacactggt gccgctggta ggtgaaaaac acgccaaagg gttgttcggc
    35821 actatcgtcg acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc
    35881 cttgccacgc cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg
    35941 caactggacg ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc
    36001 ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg
    36061 ctgggttggg tgttcattgt cagcggtgcc agcttcatca tgaactactt caccgattcg
    36121 gtggggatgt tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa
    36181 ggcggcttcc cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttatgctatc
    36241 cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc
    36301 ggcatggtgc tggggctgac agcgtcaacc tggatcctgt ggactgtact cggtagtaac
    36361 actctgctgt tgatagataa aaacatcatc aacattccaa atctgatcga acagtacggt
    36421 gtggcgcgcg ccatcattga aacctgggcc gctctgccac tcagcaccgc caccatgtgg
    36481 ggcttcttca tcctctgctt tattgccacc gttacactgg ttaacgcctg ctcttatacc
    36541 ctggcgatgt ccacttgccg cgaagtacgc gatggtgaag aaccacctct gctggtgcgt
    36601 atcggttggt caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg
    36661 aaaccgattc aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg
    36721 gtgacgctct cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa
    36781 atatcaagag gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc
    36841 ggtatccgcg aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt
    36901 gacagcgtct acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg
    36961 ctgatccctg aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg
    37021 atggagctgg gacgtctggg ggcaccaacc tatgtgctgt accagttgcc gggcgggttc
    37081 aacaccttcc tgcgcgaagg cacacaagag cagatcgaca aaattatggc tttccgcggc
    37141 accggtaagc agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt
    37201 agcctgaaaa cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt
    37261 tttattacca gcagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct
    37321 ccggacaaac ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg
    37381 accaaacttg aaaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgac
    37441 gtggaactgg acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa
    37501 gaagagttcg accatgaacg tttcctggta gccctcacca actacggtac ggcgatgtgc
    37561 gcttttgaag atgcggcgcg ctacgccaat cagcgcgtgc agtttggcga ggctattggt
    37621 cgtttccagt tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa
    37681 aacatgctgt atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca
    37741 gcgatgtgca aatacttctg cgccaatgcg gcatttgaag ttgtggatag cgcaatgcag
    37801 gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca gccgcttctg gcgtgatctg
    37861 cgtgtagacc gcgtctccgg gggatctgac gaaatgcaga tcctgacgct gggtcgcgcg
    37921 gtgctgaagc aataccgcta agtccgtgcg ctgcctgatg cgacgctaat gcgtcttatc
    37981 aggcctacgg gagcacgaat gtaggccgga taaagcgttt acgccgcatc cggcagtcat
    38041 gcactacata aatttttcag gagctaataa tggatcatct acccatgccg aaattcgggc
    38101 cgttggccgg attgcgcgtt gtcttctccg gtatcgaaat cgccgggccg ttcgccgggc
    38161 aaatgttcgc agaatggggt gcggaagtta tctggatcga gaacgtcgcc taggccgaca
    38221 ccattcgcgt tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt
    38281 taaatatttt caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata
    38341 tcttcatcga agccagcaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac
    38401 tgtggcagca caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca
    38461 ccgaggagta caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc
    38521 tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact
    38581 tttctggcct gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg
    38641 gtaaaggcga aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact
    38701 tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc
    38761 cctactacgc cggttgcggt ctgtataaat gcgccgacgg ctacatcgtg atggaactgg
    38821 tgggcattac ccaaattgaa gagtgcttta aagatattga cctcgcacat ctactcggta
    38881 cgccggaaat cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac
    38941 tggtagaaga aaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac
    39001 gctttgccga actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca
    39061 atccacagta tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct
    39121 gcaaagggcc gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa
    39181 tgccctcaca tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg
    39241 acattcagg
//
LOCUS       DAJTVJ010000027        39133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000027 DAJTVJ010000000
VERSION     DAJTVJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.027"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..139
                     /locus_tag="OO448_001323"
     CDS             <1..139
                     /locus_tag="OO448_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR6336578.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
     gene            97..774
                     /gene="bglJ"
                     /locus_tag="OO448_001324"
     CDS             97..774
                     /gene="bglJ"
                     /locus_tag="OO448_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6336579.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(812..1600)
                     /gene="fhuF"
                     /locus_tag="OO448_001325"
     CDS             complement(812..1600)
                     /gene="fhuF"
                     /locus_tag="OO448_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6336580.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            1741..1977
                     /locus_tag="OO448_001326"
     CDS             1741..1977
                     /locus_tag="OO448_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6336581.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2016..2102)
                     /locus_tag="OO448_001327"
     tRNA            complement(2016..2102)
                     /locus_tag="OO448_001327"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2066..2068),aa:Leu,seq:cag)
     gene            complement(2137..2223)
                     /locus_tag="OO448_001328"
     tRNA            complement(2137..2223)
                     /locus_tag="OO448_001328"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2187..2189),aa:Leu,seq:cag)
     gene            complement(2252..2338)
                     /locus_tag="OO448_001329"
     tRNA            complement(2252..2338)
                     /locus_tag="OO448_001329"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2302..2304),aa:Leu,seq:cag)
     gene            complement(2606..3637)
                     /gene="rsmC"
                     /locus_tag="OO448_001330"
     CDS             complement(2606..3637)
                     /gene="rsmC"
                     /locus_tag="OO448_001330"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6336582.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            3740..4153
                     /gene="holD"
                     /locus_tag="OO448_001331"
     CDS             3740..4153
                     /gene="holD"
                     /locus_tag="OO448_001331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6336583.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4122..4568
                     /gene="rimI"
                     /locus_tag="OO448_001332"
     CDS             4122..4568
                     /gene="rimI"
                     /locus_tag="OO448_001332"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6336584.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4583..5260
                     /gene="yjjG"
                     /locus_tag="OO448_001333"
     CDS             4583..5260
                     /gene="yjjG"
                     /locus_tag="OO448_001333"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6336585.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5646..6863
                     /locus_tag="OO448_001334"
     CDS             5646..6863
                     /locus_tag="OO448_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029397014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6336586.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGEITGEPL
                     KRPIIEPVEELIKYAISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYLAWW
                     ERIESIITCSDSN"
     gene            7050..10877
                     /locus_tag="OO448_001335"
     CDS             7050..10877
                     /locus_tag="OO448_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HCR6336587.1"
                     /translation="MQFITNGPDIPDELLQAHEEGRVVFFCGAGISYPADLPGFEGLV
                     ERIYQLNGTTLSEIEREAFNRGQFDATLDLLERRLPGQRIAVRRALEQALKPKLHNRG
                     ATDTQTALLRLARSRDGALRLVTTNFDRLFHVAAKRTTQAFQAYVAPMLPIPKNSRWD
                     GIVYLHGLLPEKTDDTALNRLVVTSGDFGLAYLTERWAARFVSDLFRNYVVCFVGYSI
                     NDPVLRYMMDALAADRRLGEVTPQVWALGECKPGQEHRKSIEWEAKGVTPILYTVPAD
                     TNDHSAMHYTLCAWADIYRDGIQGKEAIVVKHALARPQDSTRQDDFVGRMMWALSDKS
                     GLPAKCFAELNPAPPLDWLLKAFSDERFQYSDLPRFCVSPHAEIDPKLRFSLVQRPAP
                     YELAPQMSLASGCVSVSKWDDVMSHIARWLVRYLGDPRLIIWIAARGGQIHDRWMLLI
                     ESELDRLAALVRERKTSELDEILLHSPKAIPSPPMFTLWRLLLSGRVKSPLQNPDLYR
                     WQNRLKNEGLTTTLRLELRELLSPKVMLRRPFRYREDDSSSTDEPLRIKQLVDWELVL
                     TADYVHSTLFDLADDSWKSSLPYLLEDFQQLLRDALDLLRELGEADDRHDRSHWDLPS
                     ITPHRQNRGFRDWVSLIELLRDSWLAVRAKDSDRAMRIAQNWFELPYPTFKRLALFAA
                     SQDNCIPPERWVNWLLEDGSWWLWATDTGREVFRLFVLQGRHLTGIAQERLETAILAG
                     PPREMYQDNLEADRWHYLVAHSVWLCLAKLRGAGLVLGESAATRLTEISTAYPKWQLA
                     TNERDEFSHWMSGTGDPDFEESLDVDIAPRKWQELVQWLAEPMPERLPFYEDTWSDVC
                     RTRFFHSLYALRKLSQDDVWPIGRWCEALQTWAEPGMILRSWRYAAPLVLDMPDAVLQ
                     EISHAVTWWMEEASKSILCHEKILLALCSRLLMIETSPESSTIRNGIETYNPVSTAIN
                     HPIGHVTQSLITLWFKQNPNDNDLLTAELKPLFTKLCNVQIEQFRHGRVLLGSRLIAF
                     FRVDRPWTEQYLLPLFAWSNPIEAKAVWEGFLWSPRLYEPLLIAFKSDFLESANHYSD
                     LGEHRQQFATFLTYAALGPTDGYTVEEFRTAISALPQEGLEGAAQALYQALEGAGDQR
                     EDYWKNRVQPFWQQVWPKSRNLATPRISESLTRMVIAARGEFPAALAVVRDWLQPLEH
                     PSYDVRLLLKSDICSRYPADALSLLSSVIADQQWGPRELGQCLLQIVQAAPQLEQDVR
                     YQRLNEYSRRRCV"
     gene            complement(11319..11855)
                     /gene="yfdR"
                     /locus_tag="OO448_001336"
     CDS             complement(11319..11855)
                     /gene="yfdR"
                     /locus_tag="OO448_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6336588.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDAVIR
                     EKYRLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFSVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(11983..12807)
                     /locus_tag="OO448_001337"
     CDS             complement(11983..12807)
                     /locus_tag="OO448_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HCR6336589.1"
                     /translation="MSQNLNATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(12873..13253)
                     /locus_tag="OO448_001338"
     CDS             complement(12873..13253)
                     /locus_tag="OO448_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336590.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            complement(13958..14650)
                     /locus_tag="OO448_001339"
     CDS             complement(13958..14650)
                     /locus_tag="OO448_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6336591.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDFKSNTVCKNSISEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            14748..15008
                     /locus_tag="OO448_001340"
     CDS             14748..15008
                     /locus_tag="OO448_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6336592.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEISEMQILYPARFQSSQNQNGFKPQEQEISRG"
     gene            15001..15558
                     /gene="ymfL"
                     /locus_tag="OO448_001341"
     CDS             15001..15558
                     /gene="ymfL"
                     /locus_tag="OO448_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCR6336593.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            15734..15913
                     /locus_tag="OO448_001342"
     CDS             15734..15913
                     /locus_tag="OO448_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6336594.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            15903..16844
                     /locus_tag="OO448_001343"
     CDS             15903..16844
                     /locus_tag="OO448_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6336595.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDAPQP
                     DTKTAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRNHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAGFRG"
     gene            16841..17335
                     /locus_tag="OO448_001344"
     CDS             16841..17335
                     /locus_tag="OO448_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6336596.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTCRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            17335..17988
                     /locus_tag="OO448_001345"
     CDS             17335..17988
                     /locus_tag="OO448_001345"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR6336597.1"
                     /translation="MSNKYCQALVELRNKPAHELKDVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            17985..18311
                     /locus_tag="OO448_001346"
     CDS             17985..18311
                     /locus_tag="OO448_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6336598.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAMKDDDSEAVGIIRALLAGEENSRLRAAHWLH
                     ERGLKV"
     gene            18308..18697
                     /locus_tag="OO448_001347"
     CDS             18308..18697
                     /locus_tag="OO448_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6336599.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            18717..19514
                     /locus_tag="OO448_001348"
     CDS             18717..19514
                     /locus_tag="OO448_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6336600.1"
                     /translation="MNNLMVIDGIEVRRDAFGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTTINN
                     IKKFWSLTAKGCMFGKNIISPANPRETQPHFFESRFPELLKLLDTVH"
     gene            19522..20511
                     /locus_tag="OO448_001349"
     CDS             19522..20511
                     /locus_tag="OO448_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6336601.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            20525..21277
                     /locus_tag="OO448_001350"
     CDS             20525..21277
                     /locus_tag="OO448_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6336602.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
     gene            21531..21734
                     /locus_tag="OO448_001351"
     CDS             21531..21734
                     /locus_tag="OO448_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336603.1"
                     /translation="MLKQQDMTETAAAVLHFLPADKWVTPRMMTRTTGVSEARCQLIL
                     TQLVLAGLAKDNGGYGNKFRRCQ"
     gene            21885..22937
                     /locus_tag="OO448_001352"
     CDS             21885..22937
                     /locus_tag="OO448_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR6336604.1"
                     /translation="MKNTVKINSVDLINADCLHFIQSLPDDSIDLIVTDPPYFKVKPN
                     GWDNQWKGDEDYLKWLDHCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERVLFAEHYQGPYRGKSDGYAAKERELKQHIMA
                     PLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQPHHQLVASYDSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWMHKPV
                     QFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELES
                     ERFNQTVKEINELVGK"
     gene            23004..23219
                     /gene="essD"
                     /locus_tag="OO448_001353"
     CDS             23004..23219
                     /gene="essD"
                     /locus_tag="OO448_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCR6336605.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            23219..23716
                     /gene="rrrD"
                     /locus_tag="OO448_001354"
     CDS             23219..23716
                     /gene="rrrD"
                     /locus_tag="OO448_001354"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCR6336606.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            23713..24180
                     /locus_tag="OO448_001355"
     CDS             23713..24180
                     /locus_tag="OO448_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6336607.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRSADALDAKYTKELADAKAENDALRRKLDNGGRVFVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRTLQEYIRTQCLR"
     gene            24168..24320
                     /locus_tag="OO448_001356"
     CDS             24168..24320
                     /locus_tag="OO448_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336608.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSESRLNISLLMLDIEHLA
                     RGKGLR"
     gene            24996..25490
                     /locus_tag="OO448_001357"
     CDS             24996..25490
                     /locus_tag="OO448_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HCR6336609.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            25490..27592
                     /locus_tag="OO448_001358"
     CDS             25490..27592
                     /locus_tag="OO448_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6336610.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            27589..27801
                     /locus_tag="OO448_001359"
     CDS             27589..27801
                     /locus_tag="OO448_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336611.1"
                     /translation="MNQNDIEAMIQRYMEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            27801..29309
                     /locus_tag="OO448_001360"
     CDS             27801..29309
                     /locus_tag="OO448_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6336612.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKDYEDYELTAARIAAALGMYIRKG
                     DGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSED"
     gene            29254..31281
                     /locus_tag="OO448_001361"
     CDS             29254..31281
                     /locus_tag="OO448_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR6336613.1"
                     /translation="MPQRNDRSRSTPTTSPKINSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVNENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSGYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            31368..31691
                     /locus_tag="OO448_001362"
     CDS             31368..31691
                     /locus_tag="OO448_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HCR6336614.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGLTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            31684..31959
                     /locus_tag="OO448_001363"
     CDS             31684..31959
                     /locus_tag="OO448_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HCR6336615.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQHWTVTRHERFNGKPMIFIE"
     gene            31971..32549
                     /locus_tag="OO448_001364"
     CDS             31971..32549
                     /locus_tag="OO448_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6336616.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLTRR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            32546..32947
                     /locus_tag="OO448_001365"
     CDS             32546..32947
                     /locus_tag="OO448_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6336617.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            32958..33701
                     /locus_tag="OO448_001366"
     CDS             32958..33701
                     /locus_tag="OO448_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6336618.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            33762..34148
                     /locus_tag="OO448_001367"
     CDS             33762..34148
                     /locus_tag="OO448_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6336619.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGTIDNAGDDEHEALTPEKS"
     gene            34157..34486
                     /locus_tag="OO448_001368"
     CDS             34157..34486
                     /locus_tag="OO448_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6336620.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            34458..37523
                     /locus_tag="OO448_001369"
     CDS             34458..37523
                     /locus_tag="OO448_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6336621.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEESTRQKVAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            37523..37852
                     /locus_tag="OO448_001370"
     CDS             37523..37852
                     /locus_tag="OO448_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6336622.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            37862..38560
                     /locus_tag="OO448_001371"
     CDS             37862..38560
                     /locus_tag="OO448_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6336623.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            38566..>39133
                     /locus_tag="OO448_001372"
     CDS             38566..>39133
                     /locus_tag="OO448_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6336624.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     EYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAA"
BASE COUNT         9622 a         9195 c        10941 g         9375 t
ORIGIN      
        1 ggcaattcac agcaacgctg aaatgtatgc atggataaat agcgcgcagg gtgcaagaga
       61 acttaacttg ccttctgttt atggagatgc cgcagaatgg aacacagccg aattaagaag
      121 agaaatgtcg cactcataga aaaatgcgtc atgagcagta tcggtattga gagtttattc
      181 agaaagtttg cgggtaaccc ttataagctc catacctata ccagtcagga gtcatttcag
      241 gatgccatgt cgcggatctc atttgcggcg gtcatttttt ctttttctgc catgagaagt
      301 gagcgcagag agggattatc ttgcctgact gaactggcga ttaagtttcc gcgtacccgg
      361 cgtttagtta ttgcggatga tgatattgaa gctcggctga ttggttcatt gtcgccatca
      421 ccgctggacg gtgtattaag taaagcgtca acgctggaga tttttcatca ggaactcttt
      481 ttgtcattaa atggtgtacg tcaggcgacc gaccgactga acaatcagtg gtacattaac
      541 caaagccgga cgctaagccc gacggagaga gaaatattgc gctttatgtc gcgtggctac
      601 tcaatgacac aaattgccga gcagcttaaa cgcaatatca aaacgatccg tgcacataaa
      661 tttaatgtga tgtcgaaact gggcgtcagt tctgatgcag ggttgttgga ggccgcagat
      721 attctgttat gtatgcggca ttgcgaagca agtaatgtgt tgcatcccta ttaatccgca
      781 tgatgccggg tttacttccc cggcagtgct ttcatttcag cgtacaatcg ccacattgct
      841 gcacatccgg taagcgataa cgctggcagc aggttcggcg caccagcagg ccatcgcgca
      901 gcacgacggt acgccagagt ggattatctt caccgttcgt gagcgttttc tcaaaaaaga
      961 gggcatggcg cagcgattca accgtcgcct cgccgagcag ttgcttcatc tcagtgagat
     1021 accagttgat caaataaccg gtattactcc agataagttt gccgttgatc tctccggtcg
     1081 cttctagtgc ctgcacaacc ggaaccagcg cctggctgat taacgtttcc attcgctgct
     1141 gcggcgaatg tggtgttgcg tttttatctt cacacacatc gacccagaaa caggcgacgc
     1201 gtccggtttc gtgaaactca gcatggaaat gttccggcga cacatctaat gccttttcct
     1261 gcgtcagtag cgccagcatt aatggtggca ccatcaggcc gatatacaat tgtgcccata
     1321 gtgagatcag cggtttgttc tcgcggatca tcgttggttg gttgcgatag atatgatcgg
     1381 aatagaccgc gagtagagaa cttagcgcat tcggtgatga ccattgcgcc agcgtcatgg
     1441 cgttaagcgg agcgggttca tccaggcgga taaactcgag caaatgttca cgatgtttgg
     1501 cgatcgtcgc acgtacggct tgtgcaagcc cagcatcctg cggctggaga tgcgttcgcc
     1561 agatgatatc ttcatagagc ggtgcggaac gataggccat aatcgggata gtaatctaaa
     1621 tgataatgat tgctaatcct agcgataggt ttacccgata gcaagggatt tctctggctt
     1681 gcaaatgata aaaattatca tatgatattg gttatcatta tcaatgaaag agatgaaacc
     1741 atgttgcaac gtacgctggg cagtggctgg ggagtgttgc tgccgggatt gctgattgca
     1801 gggctgatgt atgcggattt atcgccggat cagtggcgga ttgtcattct gatgggatta
     1861 gtattgacgc cggtgatgct gtatcacaaa cagttacggc attacgtttt gctaccatcg
     1921 tgcctggcgc ttattgctgc catcatgctg atgataatga atttgaatca gggatgaaaa
     1981 atcaaggaag aaacaagaaa ggaagtaaag ataattggtg cgaggggggg gacttgaacc
     2041 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
     2101 gcacagtcat cttacttttt ttgatatcgc ctcgtttggt gcgagggggg agacttgaac
     2161 ccccacgtcc gtaagaacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     2221 cgcccagtgc gagcaatatc aacgtggttt ttggtgcgag gggggggact tgaaccccca
     2281 cgtccgtaag gacactaaca cctgaagcta gcgcgtctac caattccgcc accttcgcat
     2341 accatcaatt cttaaaaaga attgctacca cggaggcgca ttctagtggt tttcagcttt
     2401 tcgtcaatag ttaattatcg ccggaggtgt aattgctgga aaaatgtcca tcaggaaact
     2461 agcgtgcagg tttggtaggc atgcgggggc agatgccaga tgcgacgctg gcgcgtctta
     2521 tctggcctac gaagggctaa cgtgcaggtt ttgtaggtcg gataaggcgt tcacgccgca
     2581 tccgacacgg tattcggcga gataattaac ctttcttcgc ctggcgggtc ataatggcgc
     2641 gatacacctt gaaacgcccg gtttgcgcaa tcacttcatg gaagccaaat gtctcatcca
     2701 gcacgtccgg gtaaggcagg aaggcgttcg ctacaattcg cagctcgccg ccgctattaa
     2761 gatgacgcac cgcgccgcga atcagcgttt gcgccgcatc caggctggtt tgcatcccat
     2821 cgtggaacgg cgggttggag atgatcatat caaaacgacc tttcacctcg gaaaagacgt
     2881 tgctggcaaa gacttcacct tcaacacagt tggccgcaag tgttgcgcgg ctggcttcta
     2941 ccgctggcgc agagacatcg cacaaggtga gacgaatttt cggcgaatgg cgcgcaaagg
     3001 caactgaaag cacccccgcg ccacagccga catccagcac tttacctttc gtgtgcggag
     3061 ttaacgtcga gagcagcaac tggctaccga catccagacc gtcgcggcta aacacgccag
     3121 gcagcgtttt gaccgtcagg ccatcgacgc tgtattcgcc ccagaattta tcggcatcaa
     3181 ataccggctg tttttccaga cgaccaaaat agaggccaca gcgacgagcg ctgtcgactt
     3241 tattcaacgg cgcataatct gccagcatct gctcggcgct gcgcacgccg ctgcggttct
     3301 cgccaacgac aaaaatatct gtccccactg gcagcagaga aagtaaattc atcaactgga
     3361 actgggcttc cggtttgttc ttcggccagt agtaaatcag catatcgcaa tctgcgacgt
     3421 catccaccgt ggcgaccaga ctgaaacggg cgttatcccc catctggcgg cttaatacct
     3481 gccagtggtg gaattgctgg gtatgagcac ggctggccgc ggtatctaaa cgcgcgggca
     3541 ggtcatcctg taagtctccg gcaaacagaa tacggctttg ttcgaaatca tcactgtgac
     3601 gcagcaagac ttcacttgcc ggggtaaatg cagacatgga atgctcctca attgatactg
     3661 gcggcgatta tagccatatg ttggcgcggt atcgacgaat ttgctatatt tgcgcccctg
     3721 acaacaggag cgattcgcta tgacatcccg acgagactgg cagttacagc aactgggcat
     3781 tacccagtgg tcgctgcgtc gccctggcgc gttgcagggg gagattgcca ttgcgatccc
     3841 ggcacacgtc cgtctggtga tggtggcaaa cgatcttccc gccctgaccg atcctttagt
     3901 gagcgatgtt ctgcgcgcat taaccgtcag ccccgatcaa gtgctgcaac tgacgccaga
     3961 aaaaatcgcg atgctgccgc aaggcagtcg ctgcaacagc tggcggttgg gtactgacga
     4021 accgctatca ctggaaggcg ctcaggtggc atcaccggcg ctcaccgaat tacgggcaaa
     4081 cccaacggca cgcgccgcgt tatggcaaca gatttgcaca tatgaacacg atttcttccc
     4141 tcgaaacgac tgatttacca gcggcttacc acattgaaca acgcgcccac gcctttccgt
     4201 ggagtgaaaa aacctttgcc agcaaccagg gcgagcgtta tctcaacttt cagttaacgc
     4261 aaaacggcaa aatggcggcg tttgcgatta cgcaagtggt gctggatgaa gctacattgt
     4321 tcaatattgc ggtcgatcct gactatcagc gtcagggatt gggaagggtg ctgctggaac
     4381 atctgatcga cgaactggaa aaacgcggcg tggcgacact atggctggaa gtccgtgcct
     4441 caaatgctgc cgccattgcc ctgtacgaaa gtttaggctt taacgaggcg acgattcgcc
     4501 gcaattacta ccccaccacg gacggtcgcg aagacgccat catcatggcg ttgccaatca
     4561 gtatgtaaga caaggtggaa taatgaagtg ggactggatt ttctttgatg ccgatgaaac
     4621 gctgtttacc tttgactcat tcaccggcct gcagcggatg tttcttgatt acagcgtcac
     4681 ctttaccgct gaagattttc aggactatca ggccgttaac aagccactgt gggtggatta
     4741 tcaaaacggc gcgatcactt cattacagct tcagcacggg cgttttgaga gctgggccga
     4801 acggctgaaa gttgaagcag gcttgcttaa cgatgccttt attaatgcga tggcggaaat
     4861 ctgcacgccg ctgccgggcg cggtttctct gcttaacgcc attcgtggca acgccaaaat
     4921 cggcatcatc accaacggct ttagcgcctt gcagcaagtg cgtctggaac gcacgggcct
     4981 gcgtgattac ttcgatttgc tggtgatttc cgaagaagtt ggcgttgcca aaccgaataa
     5041 gaaaattttc gattatgcgc tggaacaggc gggcaatcct gaccgttcac gcgtgctgat
     5101 ggttggcgac actgccgagt ccgatattct cggtggcatc aacgccgggc ttgcgacctg
     5161 ctggctgaat gcgcacaatc gcgagcaacc agaaggcatc gcgcccacct ggaccgtttc
     5221 ttcgttgcac gaactggagc agctcctgtg taaacactga ttgcctcccc cccgttgatg
     5281 ggtaaaatag ccgcaatttt tcgttttcaa caagcgcggc gcgatgccgc ttactcaaga
     5341 agaaagaatt atgacgttgt ctccttattt gcaagaggtg gcgaagcgcc gcacttttgc
     5401 cattatttct caccccaata attaagccca aattaaagct cttttactcc ttcaaaatcc
     5461 tttcagttaa ttgatgttga gctatataaa tcatttaaat acatttggtt atgtgacttc
     5521 tttaattgaa aattctttca tgtaacttta aatcgatcag gttgctttca tcaaaaatct
     5581 gtacatatgc ttgtacataa tgtacaaagc agcagaggtg ttttgcgatt tgtacaaggt
     5641 gagtaatggc gctgtctgat gcgtggttgc gttcagtcgt tggaaaggaa cgtgataagg
     5701 ttttggttaa atccgatcgt gatggtctgt ctgtcagagt atcaccgaaa ggtcgcgtag
     5761 tgttccaata tcgttatcaa tgggcaggga aaggtgagcg tcttgatatc gggacttacc
     5821 cggcaactgg attaaaagag gccagagaag aagttatccg tcttcgtggt gaactcgagt
     5881 caaaccgtaa tccacgattg gtcaagcagg ctgaaaaacg aaaagctact gaagccatga
     5941 cggtagagtc tgtgatccgt gcctggtatg aagcatattg tgtaaaaaat aaaaaaggtt
     6001 ctgaacagat actccgctcg tttgagctgc acctgttttc taaaatcggg aatatccctc
     6061 acgatgcagc tacattgcat gattggttag aagtcctgga gcctcttagc actaagactc
     6121 cagcaatagc agaccgattg ctaattaacg caaagcaggc ccatgtctgg gcgtataaga
     6181 gaaagctcat tgaaactcgc ccgctgtcgg atatcacggg taaagatatg gatatccgta
     6241 aaggtcagaa gaaacggttt ctgacacatg atgaaattaa aatcctttat gctgcgatcg
     6301 atggttctcg aatggttcct aaataccggg ccttcattaa actattgctg cattttggtt
     6361 gccgtagttc agagctaatt accgcccggg tggacgattt tgatttcatt aataaagtat
     6421 ggactgtacc accagaacga cataagactg gggagataac aggcgaaccg ctaaagcggc
     6481 ccattattga accggttgaa gagcttataa agtacgctat ctctatgaac aatggttccg
     6541 atatgctttt tactaaggaa ggaagcaggg aacccgttgg tcggacatca ttgcagtcgc
     6601 tgccttacaa tttaatgcag tacgcatggc ggcgtttggg gtatcaattc cctcattggt
     6661 ctcttcatga tttgaggcgc acagcacgaa caaacttttc tgatcttact gcgcctcata
     6721 ttgcagaaat aatgctcggt cataaactgc cgggggtatg gcaagtttat gataagagcg
     6781 attatctaga agaacagcgt aaagcttacc tggcatggtg ggagaggatt gaatcgataa
     6841 ttacttgctc agactctaac tgagccttga gtagccataa tacaatcaaa tctagccgtt
     6901 cgctttgata ggagagcgga cattgtgatt tggctagatc agggtagctt gcaaggaagc
     6961 agtgtttgca tagtcggtct aatcatctat agccccattg tcccctaatt tgctcgaatg
     7021 aacatacaca ttggatagga ggtaaaggaa tgcaatttat caccaacggc cctgatattc
     7081 cggatgaact tttgcaggcg cacgaggaag gccgcgtggt gttcttctgc ggggctggca
     7141 tttcctaccc tgctgatttg ccaggttttg agggattggt agagcggatt taccagttga
     7201 acggaacaac actttcagaa attgagcgtg aggccttcaa tcgaggtcag ttcgacgcca
     7261 cattggattt gttggaacgg cgcttaccgg gacagcgtat agccgtccgt cgcgcgctgg
     7321 aacaagccct taagccaaaa cttcacaata ggggcgcaac tgatactcag acggcgctgt
     7381 tgcgtttggc gcgtagccgc gatggtgccc ttcggttagt tactaccaac tttgaccgct
     7441 tatttcatgt ggcagctaaa cgcacgactc aagcgtttca ggcttatgta gcgccgatgt
     7501 tgccaattcc aaaaaacagt cgctgggacg gaatagtcta cctgcatggg ttgttaccgg
     7561 aaaagacaga cgatactgct ctgaatcgtc tggttgtcac tagtggcgac tttggcttag
     7621 cctatctcac ggaacgctgg gctgcccgtt ttgtaagtga cttgttccgt aactacgtgg
     7681 tctgcttcgt gggctacagc atcaacgacc cggtgctacg ctatatgatg gacgcactgg
     7741 cagcagatcg gaggctcggt gaagtcacgc cgcaagtatg ggcactagga gaatgtaagc
     7801 cggggcagga gcaccggaaa tctattgagt gggaggccaa gggggtcaca cccatccttt
     7861 acaccgtacc ggcggacacc aatgatcatt cagcgatgca ctatacattg tgcgcttggg
     7921 cagatattta tcgcgatggt atacagggca aagaagccat tgtcgtcaaa catgctctgg
     7981 cccgcccgca ggatagcaca cgacaggacg atttcgttgg tcggatgatg tgggcattat
     8041 cagataagtc aggtttacca gcaaaatgtt ttgcggaact caatcctgca ccgccgctgg
     8101 attggttatt gaaagctttc tcggacgaac gatttcaata cagcgatctg ccacgctttt
     8161 gtgtatctcc gcatgccgaa attgacccga aactccgatt cagtctggtt cagcgtcctg
     8221 cgccctatga gctagccccg cagatgtcgc tggcttctgg atgtgtcagt gttagcaaat
     8281 gggatgacgt aatgtcccat atagcccgtt ggctagttcg ttatctgggc gaccctaggt
     8341 tgatcatatg gattgctgca cgcggcggac agatacacga ccgttggatg cttctgattg
     8401 agagcgaact agatcgctta gcagcactgg tgcgggagcg caagacttct gagttggatg
     8461 aaattctctt gcattccccc aaggctattc ctagtccacc aatgtttact ttatggcggc
     8521 ttctgcttag tggtcgtgtg aaatcgccat tgcagaaccc agatctgtat cgttggcaaa
     8581 accgcttaaa gaatgaaggc ttgacgacta cattgcgctt ggagttacgt gagttacttt
     8641 ctcccaaggt gatgttgagg cggccgtttc gctatcgtga agacgattcg agcagcactg
     8701 atgagccctt gcgaatcaag caattggtgg actgggagct ggtgctgact gctgattacg
     8761 tacattcaac cctgttcgac cttgctgacg attcatggaa atcgtccttg ccatacctgt
     8821 tggaagattt tcagcagttg ttgcgtgatg cactggactt gttgcgggag ttgggagagg
     8881 ccgacgatcg tcacgaccgc tcgcattggg atttgccgtc catcactccg cacaggcaga
     8941 accgggggtt ccgcgattgg gtgagcctga ttgaattact tcgagattca tggttagccg
     9001 ttcgagctaa agacagcgat cgggctatgc gcattgctca gaattggttt gagttgccat
     9061 atcccacctt caaacgtctg gccctgtttg ccgcaagcca agacaactgt ataccacctg
     9121 agcggtgggt taattggttg ttggaggacg gttcatggtg gttgtgggcc acggatactg
     9181 ggcgagaggt attcagactg tttgttttgc aggggcgaca tctgacagga attgcacaag
     9241 agcgtctgga aactgctatc ttggcggggc ctccgcgcga gatgtaccaa gataatttgg
     9301 aagcagacag gtggcattat ttggtggctc attccgtctg gttgtgtcta gcgaagctca
     9361 ggggagcggg cctcgttttg ggagagtctg cggctacacg tttgacggaa atatccacag
     9421 catacccaaa atggcaactg gctaccaacg agcgtgatga attctctcac tggatgagcg
     9481 gaaccggtga tccagacttc gaggagagtc tagatgtcga cattgcgccc cgtaagtggc
     9541 aggaactagt acaatggctc gcagagccta tgccagaaag actgcctttc tatgaagaca
     9601 cttggagtga tgtttgccgt acgcgctttt ttcacagtct gtatgcgtta cgtaaactat
     9661 cacaagatga tgtgtggcct attggtcggt ggtgtgaagc tctgcagact tgggctgaac
     9721 cagggatgat tttgcgttcg tggcggtacg ccgcaccgtt ggtgcttgac atgcctgacg
     9781 cagtacttca ggagatttca cacgctgtca cttggtggat ggaggaggct tcgaagtcca
     9841 tcctctgcca cgagaagatt ctactggccc tttgtagtcg gcttctgatg atagaaacaa
     9901 gcccagagtc tagcactatt cgaaacggaa ttgagaccta taatcctgtt tctacagcga
     9961 tcaatcatcc aattgggcat gtcacgcaat cactgatcac cctatggttc aaacagaacc
    10021 cgaatgacaa tgatttgctt actgctgaat tgaaaccact tttcaccaaa ttgtgtaatg
    10081 tacagataga gcaattccgc catggtcggg tgttgctggg gtcgcggctg atcgcatttt
    10141 ttcgtgtaga tcgaccttgg accgaacagt acctattgcc cttgtttgct tggagtaatc
    10201 ccatcgaagc aaaagctgtg tgggaaggct tcctctggtc gccacgcctg tatgaaccgt
    10261 tgctgatagc tttcaagtca gattttttgg agagcgccaa tcactattct gatcttggcg
    10321 agcaccggca gcaattcgct actttcctga cttatgcagc attgggccct actgatggat
    10381 ataccgtgga ggagttcaga acggcaatta gtgctcttcc acaagaaggt ctggaggggg
    10441 ccgcgcaggc gttataccag gcacttgaag gtgcgggcga tcagcgcgag gactattgga
    10501 aaaatcgtgt ccagccattc tggcaacagg tctggccaaa gtcccgtaac ttggccaccc
    10561 cgcgcatatc cgaatcgttg actcgtatgg tgattgctgc ccgaggtgaa tttccggctg
    10621 ctttggctgt ggtgcgggac tggctgcaac cgctcgaaca cccgagctac gacgttcgcc
    10681 ttttgctcaa atcggatatt tgcagtcgat atcctgcgga cgctctatcc ttgctgagtt
    10741 ctgtgattgc cgaccaacag tgggggcctc gagagttggg gcaatgcttg cttcaaattg
    10801 ttcaagctgc tccacaactg gagcaagatg ttcgttacca gcgattaaat gaatattctc
    10861 gaaggcgctg cgtgtgaaag tgacgagtgt cggggcgtgc gagctgtgga ggctagcaag
    10921 gtaaaaacac tcgaactaac aaggctgcgc ctctcgtgca atgcaataca gtttttgtct
    10981 ggcggtgaat tatggcatta aaagtcagcc cctgacacag gatgggtagc ccattagatt
    11041 tggctctgtg tcatagatgg gtattagctc atatgtgaga taatacccat tactcaataa
    11101 ctccagcaaa cctgtatatc ttgcgtgatg cccatttatt tgggcaggat ttaatatcag
    11161 gatctggaaa atctggtctg tatttctggc cagtcctcct gtttacgctg ttccagcgaa
    11221 gcaccgtcga tattgaaacg ccacagaatt cggcgacttg ttttgttgtc attaagttgt
    11281 tcattgctgc ccctccagtg attgttcggt tgaaagactc atgcgcattt gcgtaactcc
    11341 gataattcgt taaagcgttc cataaacatc ccgtaggcat ggcccggtgc cagtggaatc
    11401 acgctgaaca tctctgttgc cgggatgcct tccagtacag gccagaaaga gccatcatca
    11461 agcccgagat cgcggcgttc ggttgccagc atgatgagat cggcatattt cacgggcgta
    11521 ctcataaccg ggggtaacct gtatttctca cggattacgg cgtctatttt ttcttccatc
    11581 tgtttatagt caggaagaag gcgtttcagt ggagcgggga tgtcctgaca atacgcttct
    11641 gttgcatcat gcattaacgc ttcaaaagca aattcctgcg gtaccagttg gctgcaaagc
    11701 accgcatgtt gggcgacgct gtagaagtgc gaaagatgac cggcaaagcg acagatattt
    11761 gaaagggaaa ccgcgatatc gttaatatcg atgtcgtctt tatttatcct gtcataataa
    11821 aaatgcttcc cggaaaaagt tttaataaat gacattttgt tctccatgta tatgcgctgc
    11881 accgcgctga attctggtaa aaggaagccc tcaccacccg gcgattattg agtaaattat
    11941 gtttccataa atgcccctgc aggggcattt gcagtaatga aatcaggcgg tgaaagtacc
    12001 aataaaggtt tctactttgc tgtccttgaa tttctcaaca agcagatcac gaaattcgtt
    12061 agccatttct tcctgcaccg cttccagctg aataatgcgc agaaccagta caggacgatc
    12121 gccagtgata atgctgaggc gtaatttaaa cggacgttct ttcagacctt caaacggaac
    12181 gcatttaaat tcaaatgcca ctggcataat gtctttggtc ttcgcttcga cagactccat
    12241 cagggagcgt ttgccgctga agtcattatc ttcaaaatca gcggtctggt ttgcttcaat
    12301 cgtgatttta cggacagccg cagccgcttt tgttgcctga atagcgtcac cattagcatc
    12361 aaagcccaca agatagtcgg cccagtcttc aatccattct gccagtgact tctgggagtt
    12421 acgctcgccg ttaacagaca acagagcaga gaacggtgct gtctttttca gtttgagagt
    12481 ggcggtgtta tctgcgtgac ctggttcatc aatagtaccc aggttaagca cactgacggc
    12541 acgcatatta tcggcatcga taaagcagcg ggtgccttca tctgcaagat ctttagaata
    12601 acgggtaaag tcatcgatgc tggcagtgga aagtgcacca cggaaacgga agcgatttaa
    12661 attaaatttt tccagatcat gaatgcggaa attctcaggc aatgccacag catctgcacc
    12721 aatcttactg ataatttcat taacaccctg agcagaaata agggcatgga tttgattaat
    12781 tgcggttgcg tttaagttct gagacataat aagtcctcac tatataaaga tattcagtga
    12841 tgagataaat aatcagttaa ttaagaacga tattaatgac ctgctgcgcg gagttttccg
    12901 tcaggctcac cggcaagagt cagtaattgt ccctggtctt cctgcagaat agtcaggcga
    12961 ccaccgcgat tgacatacat cggcgtttcg gtggtgtctt cttcggaaat tttcccacgg
    13021 ttagtcgggc gaacatatga gagtttgtgt ttgattttca cacggttctc atcaaatggt
    13081 tcgatttcca ggttgagtga gaccttacct ttggttttcg tgttcatcac accggaagcg
    13141 acttcactga gaactgcgcc gattttggtt tcaaatacgc cgccgtccag ctccccgata
    13201 aatgcctgca catcagtact gcgttcgcta gccattttgc tgctcctcat catatcgacc
    13261 ctgcaaggcc gattagtttc tccacaaaac agagaagaac acctgcggtg gcagccgccc
    13321 ggatggattg ggttatgagc ccgtcgtccg atgatgttct tctctgtttt gtaaaaagag
    13381 cggtaccagc cggaagcaag tgtacaaact ggtaccgcca aagcagtggc tgttgtggtg
    13441 accggtgctg atctccggct tgcggttatt tcagactctc acgggcgttt aattgccccg
    13501 ccgaacagct cttttccgca atagctgcaa tgtctttcgc gcatcagcct gcgcattcac
    13561 cacaacgctg agagcactta gccagttacg gcaccacact ttgtcgcggt tccataaatg
    13621 ccctcatcgt tgcaccctgg tctcttccca ggtgtcaaac cgaaccgcca cgctggttag
    13681 gcgtcttatc agcatcatca ttgacttgca cattccggct acctggtttg tttgcccgag
    13741 caaggagtgg attgtcccct ttaacgtccc cagaccgcta acgacgcatg tgccatacgc
    13801 cgtgttacaa ccaaattttg ttagtacctt gtttgttggt ctggaaagaa agataaaatg
    13861 aggttgcgca ttatgcaagt gtttttgttg cgagatatgc aatttgatgg gtaatgaaaa
    13921 gccaccttcg ggtggctaat tgatgaggag gtaagggtta attgtgtcgc ttaagggttt
    13981 gtgactggct gattaagacc tttccaaaga ccataaaccg gtgttcattt tcgctggtaa
    14041 ttccccattc acggtaaatc tggttatcag aaatcaccag tagtttgtca ggtatcattt
    14101 gcagtcgttt gacataaatt ttatcatcaa aaccaaatac atagatacca tctccatcaa
    14161 actgattgat actgacatca acgaagatga gatctcctgg ctcaatggtt ggacacatac
    14221 tgtccccacg aacgttgata actttaatgt gatttgctgg ccgtccgcca aacatcgata
    14281 cagcattatc agttctgtat tcaatggcat gaatcacatc aatgacatca ccgccctgga
    14341 taaggccatt tcccgcactg gcactgatat ccagcatttc aatacggaat acatccttca
    14401 cctgcgcaac atcctcacta atactgtttt tacatacagt attacttttg aagtctgagg
    14461 taaagagatc agcaatatca acacctaagc tcctggcaat attactcagg gcttgttcag
    14521 tgaattgttt ctgcttacct gtttccaggc gcgagatatt tgccgcatcc actcctattg
    14581 cttcagcgag atcggcgatt ttcatgttct tcgcttggcg aagttgtctg actcgatttc
    14641 ctatgttcat gcgtttatta catttcttta ttgcgcgtta agcaaatcaa cttgcgcaaa
    14701 atatttgcgt gaaataatat gcttatcacg caatatgtgg aggttatatg caatcaccat
    14761 tacggaatgt gcgtaaggcg cacggattta ctttgcagca tgttgctgct ggcgttcagg
    14821 tcaatccagc gacgctgagt cgtattgaaa gactggaaca aattccatct atcgagcttg
    14881 cagaacgtct ggccaatttt tttaagggtg aaatcagcga aatgcagatt ctttatccgg
    14941 cacgttttca atctagccaa aaccagaatg ggtttaaacc acaggaacag gagataagcc
    15001 gtgggtaagc atcactggaa agtggaaaaa cagcctgagt ggtacgtgaa agctgtcaga
    15061 aaaactatcg cggcgttgcc gggtggttac gctgaagcgg ctgactggct cgatgtaaca
    15121 gaaaacgctt tattcaaccg ccttcgtgca gatggcgatc agattttccc gctgggatgg
    15181 gcaatggttt tacagcgtgc tggtggcact cacttcattg ctgatgctgt ggcgcagtct
    15241 gcaaatggcg tctttgtgtc gcttcctgac gtcgaggatg tggacaacgc cgatattaac
    15301 cagcgtctgc tggaagtcat tgaacagatc ggcagttatt caaaacagat tcgttcagca
    15361 atcgaagacg gtgtagtgga accgcatgag aagacagcaa ttaacgacga gctgtatctc
    15421 tcaatttcga agctgcagga gcatgcagca cttgtctaca aaattttttg catttcagaa
    15481 agtaatgacg cccgcgagtg tgcagctccg ggcgtcgtgg cgtcgattgc ttctggttgt
    15541 ggagaaacta acgcatgaac agtttaacaa cacactaccg tcgctcgcaa ctgattgcgc
    15601 ttcctgtacc gggtggaaaa gcgaaggtgg aatattgcta tgcagtgaat gtaccaggtg
    15661 acagggaaat tgtaacccac agctttgcag agtgggctgt gggtgatttc aaccggcaga
    15721 aggagacggt cctttgcgac aagttaaccg ctggttcaaa gatcactacg gagtgcccgt
    15781 cagagtcatt cgttgggagc cggaaacaca acgggttatc tacctccgcg aaggttatga
    15841 gcatgaatgc ttcagtccgc tcgaacagtt tcgtcgtaaa ttcagggaaa tagaggtcgg
    15901 tcatgagcac taaattaacc ggctatgtat gggatggttg cgctgcatca ggcatgaagt
    15961 tatccagcgt ggcaattatg gcccgcctgg ctgatttcag taatgacgaa ggtgtgtgct
    16021 ggccatcaat tgaaaccatt gcccgtcaga ttggcgcggg gatgagtacc gtcagaacgg
    16081 ctatcgcacg gctggaagca gaaggctggt taacgcgtaa ggcgcgtcgc cagggtaacc
    16141 gcaatgcgtc gaatgtttat cagcttaacg ttgcgaagct tcaggcagcg gcattttctc
    16201 aactgtcaga ttctgacccg tcaaaatctg acgcatcaaa atctgacccg tcaaaatttg
    16261 atgcgtcgaa atctggcaaa aaagcgggtt ttcacccgtc agaatctggc ggggatccgt
    16321 cagtaaaatc aaaacatgat ccgtcagata aaaaaccttc tcgtccggac gctccgcaac
    16381 cggacacgaa gacggctgaa caggattttt taactcgtca tcctgatgcg gttgtattca
    16441 gccctaaaaa gcgccagtgg ggaacgcagg atgatttgac ctgcgcacag tggctctgga
    16501 aaaaaatcat cgccctgtac gagcaggccg ccgaatgtga cggcgaggtg gttcgtccca
    16561 aagaaccgaa ctggacagcc tgggcaaacg aaattcgcct gatgtgtgtg caggatggtc
    16621 gtaatcataa acaaatctgc gagatgtaca gccgcgtcag tcgcgatccg ttctggtgcc
    16681 gtaacgtgct cagcccgtcg aagctgcggg aaaaatggga tgagctttcc ctgcgcttat
    16741 cgccgtccgt cagcacgtac accgaaaaac gcgaagaccc gtacttcaaa tccagttacg
    16801 acaacgtgga ctacagccag atcccggcag gattcagggg gtgatcatga gtctgttaaa
    16861 tgacgttcag aaattcattg aagcccatcc tgggtgtact tccggagaca ttgcggatgc
    16921 ttttgcaggt tactcacggc agcgcgttct gcagtctgca agcaagttac gtcagagtgg
    16981 gcgtgtggct caccgttgtg aaggagatac atgcagacat ttcccgcgcc tgactgagag
    17041 agcgcaggag ccggaaccac aaccagttcg tgaaaccaga cctgtgcgca atttctatgt
    17101 cggcactaac gatccccggg tgattttgtg cctgacccgc caggctgaag aactggagtc
    17161 aaggggctta taccgtcgtg ctgcaaccgt gtggatggcg gcattccgtg aaagccactc
    17221 ccagccagaa cgaaacaatt ttctggcgcg tcgtgagcgg tgcttacgga aaagcagcaa
    17281 gcgcgctgca tcgggtgaag agtggtatct gtcagggaat tacgtggggg cttaatgagt
    17341 aataaatatt gccaggcgct ggtggaactg cggaacaaac cagcccatga actgaaggac
    17401 gtgggcgatc agtggcgcac gccggataac attttctggg gaattaacac cctgtttggc
    17461 ccgtttgttc tggatctgtt cactgacggt gataacgcca aatgtgccgc gtattacacg
    17521 gcggaagaca acgcgctggc gcatgactgg tcagaacgtc ttgcggagct taaaggtgct
    17581 gcctttggta atcccccata cagccgcgcc agtcagcatg aggggcaata catcaccggc
    17641 atgcgttaca tcatgaaaca tgccagtgcc atgcgtgata agggcgggcg ctatgttttc
    17701 ctgatcaaag ctgccaccag cgaagtgtgg tggccggaag atgcagatca tattgctttt
    17761 attcgcgggc gtattggttt tgaactgcct gtctggttta tcccgaaaga cgagaagcag
    17821 gtaccgacag gagctttttt cgctggtgct attgctgttt ttgacaagac ctggaaggga
    17881 ccggcaatca gctacatcgg gcgcgatgaa cttgaggcat gtggtgaggc gtttctggcg
    17941 caggttcgcc agcaggcgga aaaactggtc agggagatgg cggcatgacg acgttaactc
    18001 aatgccagca gcaggtgctg gatatgctga tttcttatca gaaagaacgt ggcttcccgc
    18061 caaccaatca ggaggtggca accatgctgg gataccgttc ggtgaatgca gcggtggagc
    18121 atcttcgagc actggagaaa aaaggcgtca tcacgataaa gcgtggcgtg gcccggggga
    18181 taacgcttca taccgcgatg aaggacgacg acagcgaggc ggtcgggatt atccgcgcac
    18241 tgcttgccgg tgaggaaaac agcaggctgc gtgcagccca ctggttacat gagaggggcc
    18301 tgaaagtatg aagctgattc tgccttttcc gcccagcgtg aacacgtact ggcgacaccc
    18361 caacaaaggg gcgtttgctg gtaagagcct gataagcgcg gcggggcgaa aattccagag
    18421 cgcggcgtgc gcagcaatag ttgagcagtt acgtcgtctg ccgaaaccaa cgtcggcacc
    18481 tgcttcagtg gagatcgtgt tgtttcctcc ggataaccgg atccgcgatc tggacaacta
    18541 taacaaggcg ctgtttgacg ccctgaccca cgcgggtgtg tgggaagacg acagccaggt
    18601 gaaaagaatg ctggtggagt ggggaccggt tatcccggaa gggaaggtcg agatcactat
    18661 cagtaagtac gagaaaacgg cgggtgcagc cgcctgatta agaggagaaa cgaagtatga
    18721 ataatctgat ggtcattgat ggtattgaag ttcgtcgtga tgcttttggg cgttacagcc
    18781 tgaacgatct gcacagggct gccggttctc tggataagca taagcctgca ttctggctcc
    18841 gcaatgagca aactgaacgt ttaataagcg agttgcagat ttgcaactcg gtcaatatag
    18901 agccagttaa cgttattcgt ggcggaaata accaggggac gtatgtctgc aaagaactgg
    18961 tgtatgccta tgcaatgtgg atcagcccgt cattccatct taaggtgatc cgtactttcg
    19021 acatggtaac cagcgcaccg gaaaaattat ccggacaggc tgctgacaag atgcaggctg
    19081 gtgtgattct gctggacttt atgcgccggg aattaaacct gtctaactct tcagtgcttg
    19141 gtgcctgtca gaaactccag gaggctgttg gcttaccgaa tctggcaccg cgctatgcca
    19201 ttgatgctcc tgctgacgcg cctgatggct caagccgccc cacgctgtca ctgagtgcac
    19261 tgctgaagca gtatggtatc cgcctgacag ctaatcaggc atatcaccag atggcgaagc
    19321 tggggatcgt tgaacaacgt gaacgataca gccgtaccac gattaacaac atcaaaaaat
    19381 tctggtcgct gacagcgaaa ggttgcatgt tcggcaagaa catcatcagt cccgcaaatc
    19441 cgcgcgagac gcagccgcat ttcttcgaat cccgattccc tgagctgtta aagttgctcg
    19501 ataccgttca ttgaggtgac cgtgagagca ctactgaccc ctgaaatagc cccgcgtatg
    19561 gggatcgtat tgttcagacc aggttcagaa ctgatgcccc tgtttatgca ggggcgtgta
    19621 ctgctggagc ctgagccgga acgttattca tctttcgcca gtggtgccgt tcccgcggca
    19681 tcacaaccgc tggcggatga tcctgctgtt cgggccgtgt tccgcaatga ggcagtgatc
    19741 cgtcgtgctg gtggcgtgga atgtcttgaa agctggttac ttcgtgaaaa aggctgccag
    19801 tggcctcatt ccgactggca cagcgagaac atgaccacaa tgcgacacgc gccgggcgca
    19861 atccgtctgt gctggcactg tgataaccag ctgcgcgatc agttcacgga acggctggaa
    19921 tcaatggcaa cggataactg tgcccgctgg gtgttgtctg ttgtgcgtcg ggatctcggt
    19981 tttgatgaca gtcacgttgt gacaatgccg gaactgtgct ggtggctgat tcgtaatgac
    20041 ctggcggatg ccttaccgga aagtgcagcc cgtaaggcac tgagattacc gaagcctgtt
    20101 gtgccgtctg tcacccggga gagtgacctt gttccttcgg ttcctgccac cagcattatc
    20161 caggataaag cgaaaaaggt gctggcgctg aaagtggatc cggagtcgcc ggagtctttt
    20221 atgttacgcc caaaacgtcg ccgctgggtt aatgaaaagt acacacgctg ggttaagaca
    20281 cagccgtgtg catgttgtgg aaagcctgct gatgatcccc accacctgat aggccacggt
    20341 caggggggaa tgggtacaaa agcgcatgac ctctttgtgt tgcctttgtg cagaaagcat
    20401 cacgacgagc tgcatgcgga taccgtggca tttgaagaga agtatggttc ccagctggag
    20461 ctgatatttc gttttatcga tcgtgcgctg gcaataggcg tactggcgta agtggagaac
    20521 gagcatgaac cttgaagcct taccgaaata ttactccccg aaatctccaa aattgagcga
    20581 tgacgcaccg gcgacaggct ctggtggttt aacaattacg gatgtaatgg ctgcgcaggg
    20641 gatggtgcag tcgaaagcac cgcttgggtt tgccttattc ctggcaaaag ttggtgttca
    20701 ggatcctcag tttgcgattg aaggtctgct caattacgcg atggcactgg ataacccgac
    20761 attgaacaaa ttgagtgaag aaacccggtt acagatcatc ccttaccttg tgaattttgc
    20821 ctttgctgat tattccaggt ctgcggcaag taaggctcgc tgtgagcatt gtgctggtac
    20881 tggatttcat aatgtattgc gcgaagtggt gaaacactcc agaagcgggg aatctgttat
    20941 caaggaagag tgggtgaagg aactgtgtca gcattgtcat ggtaagggag aagtcagcac
    21001 agcgtgcaga gggtgtaagg gtaaaggtat tgtcctggat gaaaaaagaa cccggcttca
    21061 tggcacgcct gtttataagg tttgtgggcg ttgcaatgga aaccggttta gccgtttacc
    21121 aaccacactg gcgcggcttc atgtccagaa gctggtacca gacctgacgg attatcagtg
    21181 gtacaaagga tatgcagatg tcattgataa actggttaca aagtgctggc aggaagaagc
    21241 atatgcagag atacaattga gaaaggtgac aagataaatg gttttcgccg aagatgacga
    21301 catgatgctt gcgtttttca aaaaatatgg ataagatttt cacaacgatg ggctttgtat
    21361 gtctaccgtt gataagattt aagaacccgc cactgagcgg gtttttttgt acctgtaaac
    21421 ttggtgcagt acagtaaaca cgctggtggt cgtgaatact gactttttat cttgctggct
    21481 ttttagacaa gagttattgg tatgtcatgt taaccagaag ggaaaaagac atgctaaaac
    21541 agcaagatat gacagaaacc gccgccgcag tccttcattt cttacctgct gacaagtggg
    21601 taacgccacg catgatgacg agaactaccg gagtaagcga agcccggtgc cagttaatac
    21661 tgactcagtt agttctggcg gggctggcga aggataacgg cgggtacggg aataaattca
    21721 gacgctgcca gtaatggcgg tttcctgctg tgaaaatggg cggctggtgg gtgttggtag
    21781 cacctgccag ccattcgctc atgcttactg gtcacaagcg aaccacggcc cactgcttta
    21841 gcgcaaaagc agagtgagcc taccagagtt acgcttactg atccatgaaa aatactgtaa
    21901 aaataaacag tgttgattta atcaacgctg attgcctgca ttttattcag tccctgcctg
    21961 atgattccat tgacctgatt gttaccgatc cgccgtactt caaggtgaaa cccaacggct
    22021 gggacaatca gtggaaaggg gacgaagatt accttaagtg gctggaccac tgtctggccc
    22081 agttctggcg ggtgttaaaa cctgccggaa gcctttacct gttctgtggg catcgcctgg
    22141 catctgatat tgagatcatg atgcgtgaac gtttcaacgt gcttaaccat atcatctggg
    22201 cgaagccgtc cggacgttgg aatgggtgta ataaagaaag tctgcgcgca tattttcctg
    22261 ccacagagcg cgttctgttt gctgaacatt accaggggcc atatcgcggc aaaagtgacg
    22321 gctatgcagc aaaagaaagg gaactcaaac agcacataat ggcaccgctg atatcgtatt
    22381 tcagggatgc tcgtgccgaa ctgggtataa cggcaaaaca aattgccgaa gccacaggta
    22441 agaaaaatat ggtttcccac tggtttggtg ccagtcagtg gcagttgccg aatgaggctg
    22501 actatcggaa gttacaggca ctgttttccc gtatagcggc agagaagttt caggaacaac
    22561 aactggaaca accacaccac cagctggtgg catcttatga ttcactgaat cgtaaatatt
    22621 ctgaattgct ggatgagttt aaatctctcc ggcgctattt ctccgtatca gtctccgtgc
    22681 cttataccga tgtctggatg cataaacccg ttcagttcta cccgggtaaa catccgtgtg
    22741 agaaaccggc ggatatgctc aggcaaataa tcaatgccag tagtcgacct ggtgatctgg
    22801 ttgctgattt ttttatggga tccggttcca caataaaagc agcaatggcg ctggggcgtc
    22861 gggccttagg tgttgagctt gagtcagagc ggtttaacca gacagtgaaa gagataaacg
    22921 agctggtggg gaaataatct ggtggccacg caggtggcct ttttatttcc attacacagc
    22981 acccgcatct gcgaggtggg gttatgaaat ccatggataa gttaacaacg ggtgtcgcct
    23041 atggcacctc agcaggtagt gccggttact ggtttttaca gctgctcgat aaagtcacgc
    23101 cctcacagtg ggcagcaata ggtgtgctgg gtagcctggt atttggcctg ctgacgtacc
    23161 tgacaaacct ttatttcaag attaaagaag ataagcgcaa ggctgcgaga ggtgaataat
    23221 gcctccatca ttacgaaaag cagttgctgc tgctattggt ggcggagcaa ttgctatagc
    23281 atcagtgtta atcactggcc caagtggtga cgatggcctg gaaggtgtca gctacatacc
    23341 atacaaagat atcgttggcg tatggactgt atgtcacgga cacaccggaa aagacatcat
    23401 gctcggtaaa acgtataccg aagcagaatg caaagccctc ctgaataaag accttgccac
    23461 ggtcgccaga caaattaacc cgtacatcaa agtcgatata ccggaaacaa cgcgcggcgc
    23521 tctttactcg ttcgtctaca acgtgggtgc tggcaatttc agaacatcga cgcttcttcg
    23581 caaaataaac cagggcgata tcaaaggcgc atgtgaccag ctacgtcgct ggacatacgc
    23641 tggcggtaag caatggaaag gcctgatgac tcgtcgtgag attgagcgtg aagtctgttt
    23701 gtgggggcaa caatgagcag agtaaccgcg attatctccg ctctggttat ctgcatcatc
    23761 gtctgcctgt cgtgggcggt caatcattac cgtgataacg ccatcgccta caaagaacag
    23821 cgagataaaa aagtcagtga gctgaagcag gcgaccgcca ccattactga catgcagcag
    23881 cgccagcgtt ctgctgatgc actcgatgct aaatacacga aggagttagc tgatgcgaaa
    23941 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgtt cgtcaaagga
    24001 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
    24061 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
    24121 agcgaccaaa cagcactgag aacgcttcag gaatacatca ggacgcaatg ccttcgatga
    24181 tagcgataat tttacttatc atccttcaca tctggctctg tagacagggt ggtgatcact
    24241 tctggagtga atccagatta aacatctcat tgctgatgct tgatattgag catcttgcgc
    24301 gcggtaaggg gctgcgttga gataagagcc agtcattaca aataccagga tttagcctcg
    24361 tattcgcggg gctttttatt gccattatca aagccactcc ctacagagtg gctttgataa
    24421 tggcttatac cctacacggg ataacttaac tgatatccct tttaacggat aaaggtattc
    24481 aagcctgaca catcatgcgc tgtatcgtcg ccgtattccc gtattaacag agaccgtagc
    24541 ccgacgggga actccttctg cgagagtgtg cgggaataat caaaaacgat gcacaccggg
    24601 tttttaccgc gctaatgatt cgcgggtttg tccctcatgc tcgccagtcc tgtgcggggg
    24661 tggaagaaac aggacactca cacagattct tgtgggtacg atgctattcc tttctggatt
    24721 atcccgatgc cattcatgca gggtgctgta tcagacgttc gtcatggctg tcaggctgac
    24781 gggtcctccc ggtggggtgg cctgccacgg ggcgggagcg tcgcggaaaa aggctagttt
    24841 ttgaaatttc attcgtcatc accactgctg taattgattg atattacagt agttttattt
    24901 ttgcagtgtc gattttgatt gttttttgtt catcactaac accgtttgcc taaagttgtt
    24961 cgcaagatgc atgtttaaaa cattctggag cgggtatgga tcgagagtta aaaaatctga
    25021 cgctgaatat cagtcaactg gcggcactgt caggtgtaca tcgccagact gctgcggcaa
    25081 ggctgcaaaa tctacccgtt gcaggggggc atgaaagcaa cctcaagctt tatcgggtgg
    25141 ttgatattgt gtcggcattt ctggcattgc caccgccggt tgcagaaggc gaaatggacg
    25201 cgcatgagcg caaagcctgg tatcagtctg aacgtgagcg tcttaagttc gaacaggaaa
    25261 cggcacaact cattccggcc agtgatgtca gacgggagtt tgccatctgg gcaaaagcgg
    25321 tcgtgcaggt gctggagaca ttaccggata ttctggaacg tgactgcggt ctgcagcctg
    25381 ccgctgtgag ccgtgttcag tccattattg atgatctgcg cgatcagata gccctgcggg
    25441 tgactgaagc aggtgcggat gatgaggagg aattacagca ggaggagtaa tgctgaatca
    25501 ggaaaccgca aaggcagcac gaaccgattc aggttatatc cttcgcgcac cgagacgaat
    25561 gcgggttgct gatgccgttg ctcagtatat gcgggtgccc atgggggcag ggaactcagt
    25621 cccgtgggat ccgctggtgg caccgtatgt tattgagccg atgaactgcc tggcctcgcg
    25681 tgaatacgac gcagtgatat ttgttggccc ggcacgaacc ggcaagacta tcggcctgat
    25741 tgacggctgg gtgatttaca acgtgatttg cgatcctgct gatatgctga tcattcagat
    25801 gacggaggaa aaagcccgcg aacactccaa aaaacgactc gccagaacgt ttcgcgtcag
    25861 cccggaagtg gtcagtcgcc tgagtccgaa caaaaatgac aacaacgttt atgacagaac
    25921 attccttgct ggtaactacc tgaaaatcgg ctggccgtca gtcaatatca tgtcctcatc
    25981 agattataaa tgcgtcgcgc tgacggatta tgaccgtttt ccggaagata ttgatggcga
    26041 gggggatgct ttctctcttg cctcaaaacg taccaccaca tttatgtcca gcggtatgac
    26101 gctggtggag agttcccccg gcagggatgt gaaggatgtg aaatggcgac ggacttcacc
    26161 gcatgaggct ccaccaacca cggggatcct gtcgctctat aaccgtggtg atcgccgtcg
    26221 ctggtactgg ccctgtccac actgtggtga gtattttcag ccctgcggcg atgtggttgc
    26281 tggtttccgt gatattgccg atcccgtgct ggcaagtgag gcggcttata ttcagtgccc
    26341 ttcctgttca ggacggatta tgcctgaaca aaaacgtgag ctgaacggac gtggggtctg
    26401 gttgcgggat ggtgaatcca tcaatgcgga tggcagtcgt tatggtgatc cccgacgctc
    26461 acgtattgcg tcattctgga tggagggtcc ggcagctgct taccagacac tctcgcaact
    26521 cgtttacaaa ctgcttactg cagaacagga atacgagaca accggaagtg aagaaacact
    26581 caagacggtt atcaataccg actggggatt accttatctt ccccgcgcca gcatggagca
    26641 acgaaaaagt gaactgcttg agcagcgggc agagccagtt ccttcccgca gtgtgccgga
    26701 tggcgttaat ttccttgtgg cgacagtgga tgtgcaggcg ggacgtcatc gccgttttgt
    26761 ggttcaggta acgggctatg gcagccgtgg cgaacgctgg attattgatc gttacaacat
    26821 cacgcagtca ttgcgcggtg acagcgacgg ggagagccag cgaattgatc cggccagcta
    26881 tccggaagac tgggatgtcc tgctgacgga tgtttttcat aaaagctggc cgctggcctc
    26941 cgatccttct caacaaatgc gactgatggc aatggcggtg gactccggcg gtgaagacgg
    27001 ggtcactgat aatgcctata aattctggcg tcgttgccgt cgtgatggcc ttggtaaacg
    27061 tatttacctg tttaagggcg acagcatccg gcgcgcaaaa ctgatcactc gtacattccc
    27121 tgataacacc ggacgaacgg gccgacgggc gcaggccgca ggtgatgtgc cgctctggct
    27181 tcttcagacg gatgcactga aagaccgggt gaataacgcg ttatggcgtg actcgccagg
    27241 tcccggctat gtgcatttcc ctgactggct ggggagctgg ttttacgacg aactgacgta
    27301 tgaagagcgg agcagtgacg ggaaatggag taagcctggt cgcggtgcca acgaagcttt
    27361 tgacctgatg gtgtatgccg aggctctggt cattctgcat ggatacgaaa agatccgctg
    27421 gccggatgca ccggagtggg cgagccggga aacctggctg gagtgtgtcc aggacagtac
    27481 cgaaccgtca tcctcaccgg aaccggtatc cacgcctgtt aaaaaacaaa aacggaagaa
    27541 aacagtaact gacgatgtta acccctggct gacttccgga ggatggttat gaaccagaat
    27601 gatatcgaag ccatgattca gcgttatatg gaagctgaaa tggcggtgct ggacggaaaa
    27661 tccgtcacct ttaatggtca gcagatgacc atggaaaact tatctgagat ccggcaggga
    27721 cggcaggagt gggagcgccg ccttgcggct ctgattacac gacgacgggg gcatcccggg
    27781 taccggctgg cgaggttctg atggcaattc ttgatgatgt gattggcgtt ttttcacctg
    27841 gatggaaagc ggcaaggctg cgttcccgtg cggtgatcca ggcttatgaa gccgtaaaaa
    27901 cgacgcggac acacaaagcc cgacgggaga accgaactgc cgaccagcta agccagtacg
    27961 gggccgtgtc gttacgtgag caggcccgtt accttgataa caaccacgat ctggttattg
    28021 gtgtatttga caagctggaa gaacgggtgg tggggaaaaa cgggattatt gtcgagccac
    28081 atccggtatt acgcaatggg gccattgccc gtgatctggc agcggagatt cgcacccgat
    28141 ggagtgaatg gtctgtcagc ccggaagtca ccgggcagtt tacccgtccg atgctggaac
    28201 gtctgatgct gcgtacctgg ctgcgcgatg gtgaggtgtt tgcccagatg gtttccgggc
    28261 gcataaacag cctgacgcct tctgccggtg ttcatttctg gctggaggcg ctcgagccgg
    28321 actttattcc catgaccagt gatgagagca acaggctgaa tcagggcgtg tttgttgatg
    28381 actgggggcg tcccgaaaaa tatctggtgt ataaaagccg tcccgtatcc ggacggcaga
    28441 tggaaaccaa agaagtggat gcagagcgaa tgctgcatct taaatttgtt cgccgtctgc
    28501 accagatgcg cgggacgtct ttgttgtccg gtgtgctgat ccgcctcagt gccctgaaag
    28561 attatgaaga ttatgagctg actgcagcaa ggatcgccgc tgctctgggg atgtacatcc
    28621 gcaaaggcga cgggcagagc tatgaaccgg atggtaatgg cagcaaggat aaggaacgcg
    28681 agcttaccat tcagccaggc attatttacg atgatctgaa acccggcgaa gaaatcggaa
    28741 tggtgaagtc ggatcgcccc aatcctaacc ttgaaacttt tcgtaatggt cagttgcgtg
    28801 ccgtggcggc gggcagtcgt ctgagttttt ccagtacagc gcgcaactat aacggcactt
    28861 acagcgccca gcgtcaggag ctggttgaat ctactgatgg ctacctgatc ctgcaggact
    28921 ggtttattgg tgccgtcacc cgcccgatgt atcgtgcatg gctgaaacag gctgtggcat
    28981 ccggtgttat caggctaccc cgcgatcttg accgttcttc actgtatacc gcggtgtatt
    29041 ccggaccagt gatgccgtgg attgaccctg ttaaggaggc tgaggcctgg aaaatccaga
    29101 ttcgtggtgg agcggcgaca gaatcagact gggtacgtgc tggtggtcgt aatccggatg
    29161 atgtcaaacg tcggcgcaag gccgaaattg atgaaaaccg caagctggat ctggtatttg
    29221 ataccgatcc ggccagtgat aaaggaggca gtagtgccgc aacgaaacga caggagccgc
    29281 agcacaccga cgaccagtcc gaagattaat tcctggttca ggatgcaggc tggtcaccag
    29341 agtgacgcgg atatttatat ttatgacgag attggtttct ggggtgttac agcgaagcag
    29401 tttatcagtg atctgaatgc actgggcgat atcacccaca ttaatctcca tatcaattca
    29461 ccgggtggcg atgtctttga aggcatcgcc atttttaatg cgctgaaaac acatggtgcg
    29521 tccattaccg tttatgtcga cggtgtggcg gcgtcaatgg cgtcggtcat tgcgatggtg
    29581 ggaaacccgg tcattatgcc ggaaaacacc ttcatgatga ttcataaacc atttggcttt
    29641 acgggcggtg atgcggagga catgcgcacc tatgccgacc tgctcgataa ggttgaggcg
    29701 gttctgttac ccgcttatgc acagaaaacc gggaaaacca ccgatgaaat tgctgccatg
    29761 ctggcggatg agacctggat gtccggtgcc gaatgtctgg cacatggatt tgctgatcag
    29821 gtgacgccag ccgttaaggc aatggcatgt attcagtcaa aacgtacaga ggaatttaaa
    29881 aagatgccgg aatccattcg aaacatgatt actccgccac gcaacagtgc tccacgcgta
    29941 caggatgatg aacctgcagc ctcccggacg ccagtgcagg cagcagcacc cgtggtgaat
    30001 gaaaacagta tccgtgcgca ggtactggca gagcaaaaag cgcgtgtaaa cggtattaat
    30061 gatctgtttg ccatgtttgg cgggcgttat cagacgctgc aggctcagtg tcttgccgat
    30121 cctgagtgtt cgctggagca ggcccgcgaa aagctgttga acgagatggg gcgcgagtcc
    30181 acgccatcca ataaaaatac accggctcat atttatgctg gtaacggtaa ttttgtgggg
    30241 gacgggattc gccaggcgct gatggcgcgt gccggatttg aaaaaaccga acgtgataat
    30301 gtctacaacg ggatgaccct gcgtgaatat gcccgtatgt cactgactga acggggtatt
    30361 ggggtttccg gttataaccc gatgcagatg gtcggtgcgg cgttcaccca cagtacgtct
    30421 gacttcggta atattctgct ggatgttgcg aacaaagcca ttctgcaggg ctgggaagat
    30481 gctcctgaaa cctatgaaca atggacgcgg aaaggtcagt tgtctgattt taaaattgcc
    30541 catcgtgtgg gtatgggggg cttcagtgct ctgcgtcagg tgcgtgaagg ggcggaatat
    30601 aaatacgtca ccaccggaga taaacaggcc actattgcac tggcgaccta tggcgagctg
    30661 ttcagtatca cccgtcaggc cattatcaat gatgatctga atatgctgac cgatgtcccg
    30721 atgaaactgg gccgtgcggc gaaatccact attgccgatc tggtttatgc cattctgacg
    30781 tctaacccga aaatctccac agataatgta agtctgttcg ataaagcgaa acatgcaaac
    30841 gtactggaga gcgctgcaat ggacgtggca tcgctggata aagcccgcca gttgatgcgc
    30901 gttcagaaag agggggagcg tcatctgaat attcgtcctg cgttcgtact ggtaccgacg
    30961 gcgatggagt ctgttgctaa ccaggtcatt cgctcctcaa gtgtcaaggg ggctgacatt
    31021 aacgccggta ttattaaccc ggtgaaagat tttgcgaccg ttattgcaga gccccgtctt
    31081 gatgataaca gccagaccac cttctacctg gctgcgtcaa aaggctccga tacgattgaa
    31141 gtggcttatc tcaacggtgt ggatacgcca tatattgatc agatggaggg cttcagtgtg
    31201 gatggcgtga caacgaaagt gcgtattgac gccggtgtcg cgccagttga tcaccgcggt
    31261 ctggtgaaat gtacggcgta aacgtcgcag acaacaactc tgatggcccg taagggcttt
    31321 ttttgtacct gaaatcagcc cctgaacggg gctgtgcgga gacagttatg gcaaagaatt
    31381 ttgtagaaga aggaaaaacg gtggcgattg ttgccagtgc agccatcagc agcggagacc
    31441 tggtgcaggt gggtgatgtt tttgcggtgg cgctgaccga tattccacag ggtgaaacag
    31501 gcgacggcct gaccgaaggt gtgtttatgc tgcctaagct gaaaacggat gacatgaaaa
    31561 cgggtaagaa ggtttatctg aagtccggaa aagttcagct gactaacagc ggctctgatc
    31621 cgctggtcgg ggttgtctgg gcagatgccg gaaccagtgc agaagaagtg ccggtaaaac
    31681 tcaatgtctg atcccttttc ccggctggca gcgcgtatgg atgcgatcac ggtcagaaag
    31741 atgggaaaga cagcctcgat taatgatgtc gatatgactg tgatcccggg agaaacactg
    31801 gcagagctga atgctctgtc cggacctgcg gtctctctgg tggtgttttc ttcgggatac
    31861 cgcccacggc gcggggatcg cgttgtttat gacggacaac attggacggt cacacggcat
    31921 gaacgcttta acggtaagcc aatgatcttt attgagtaaa gaggtgtggg atgaaggggc
    31981 ttgagaatgc catccgcaat ctgaacagcc ttgatacccg tatggtgcca caggccagcg
    32041 catgggcgat aaaccgtgtg gcacagaaag cggtctcggt tgccacccgg caggttgccg
    32101 ggaataccgt tgcgggagat aaccaggtga aagggatacc cctgaaactg gtacgtcagc
    32161 gtgtccgggt gtttaaagcc agtccgtcag gaaaaatgac ggccaggatc cgcgttaacc
    32221 ggggcaatct gcctgccatt aagctgggga ccgcccgggt cagactgacc cggcgtggtg
    32281 gaaaactgca gtaccgtggc agcgtgctga aggtgggtaa atatcttttc cgggatgcgt
    32341 ttattcagca actggcgaat ggtcgctggc atgtgatgcg gcgtattgat ggcaaaaatc
    32401 gttaccccat tgatgtggtg aaaatccctc tgtccggacc gctgacacag gcatttgaag
    32461 atgcccgcga ccgcatcatt gctgcggaaa tgccgaaaca gctggggtat gcactgaaac
    32521 aacaactgag gttatggctg acccgatgaa ccgacataca caaatccgcc aggtcgtact
    32581 ggcacgcctt cgggaacagt gtggagacag cgccacgttt tttgacgggc ttccggcatt
    32641 tgttgatgcg caggaactgc ctgccgtggc ggtgtggctg agtgatgctc agtacaccgg
    32701 aaaaatgacg gatgaagatg actggcaggc tgttctgcat attgctgtct tcatccgggc
    32761 acaggcaccg gattcagagc tggatatgtg gatggagagc accattttcc cggccctgaa
    32821 tgatgtaccg gcactttccg gactcatcga caccctgatc ccactcggtt ttaactatca
    32881 acgtgataat gagatggcca cctgggcgat ggcggaaatc acgtaccaga tcacgtacac
    32941 gaattaagga ggtggcaatg accacaccaa atccactggc aaaaacgaaa ggtgcgggaa
    33001 cgacgttctg gatgtacacc ggcaagggcg atgcgtttgc gaacccttta tcggacactg
    33061 actggctgcg tcttgcgatg gtgaaggatc tgcagcctgg cgaaatgacc gctgatgcag
    33121 aagatgacac ttatctcgat gatgaagatg cagactggaa aacgacaacc caggggcaga
    33181 aatccgtcgg tgatacttcg gcgacgctgg cctggcgtcc gggtgacagc gggcagaaaa
    33241 aactggttca gttgttcgac tccggtgaag tctgcgcgtt tcgtatcaaa tatcccaacg
    33301 gtactgttga tgttttccgt ggctggctga gttcactggg taaaaccatt gcctcaaaag
    33361 acgtgatgac ccgcacagtg aaaatcagcg gtgtggggcg tccgtatctg gcagaagaag
    33421 gcactgaaac cgtgggcgtt accgggctga cggtggcacc ggcatctgcc agtgtaaaag
    33481 tgggagcaac caccacgctg acctttacag taaaacctga cggagccagt gacaaagcga
    33541 tcagtgtgca ttcgacagat ccacagactg ccacggtgac cctgaacggg cttgtggcca
    33601 cggtgaaagg cgtgaagcag ggcagtgtca gcattgtggg catgacttct gacggcgatt
    33661 ttgtggcagt ggctacggtg gctgtcagcg ccgcaggtta acaggacgat actcatcatt
    33721 tgccccgatt atccggggct tttttgcagg tggagaacat gatgtttctg aaacagggca
    33781 cgtttaatta tgaaaagcag tccgtggtgc tcagtgagct gtccgggctg cagagaattg
    33841 aatatctggc gtttgttcag cagcgaacgg caaagtttga tgccgaagag ggagaactgc
    33901 cggaggctga acgacagatt gcttttctgc ggatggggat ggatatcaat gcctggctgg
    33961 tttcccgctc actgtggaat gcggatcagt ctaaggatgt agagacgctt tgcgcatccg
    34021 ttattacaac atggtcgtat gatgccctgg gtgcgggggc ggagatggtt ctgtcgctga
    34081 gcggtatggg gaccattgat aatgccgggg atgatgagca tgaggcgctg acgccggaaa
    34141 agtcctgacg cgggaaatgc agtttgtcat gcggcttgcc cgggagttcc ggcgggcaga
    34201 ctggcggcgg atgctgtcgg aaatgtcggc cactgagctt ggtgagtggg gcgattattt
    34261 ccggatgcag agcttcagtg atgtgtggat ggatgcgcag tttgcctcgc tgaaggcatt
    34321 gatcgtgaga atggtgtccg gcagtagtga tgctgcggtg gctgatttca gccttttacc
    34381 ggaagagaac gggataccgg agcgaacgga cgaagaactg atgcatcttg gggaaggtat
    34441 ttccggaggt gtgcgttatg gaccagatag ccaacctggt cattgatttg gggattgatg
    34501 cggcagagtt taaaaatgaa atcccccgta tcaaaaacct tctgaatggt gcagccagcg
    34561 atgcagaacg gtcttctgcc cgtatgcagc gttttatgga gcgtcagact caggccgccc
    34621 ggcagacaac gcaggcggct tcttcggctg caacagccgc atccgtccat gcgcagacgg
    34681 tggagaagaa cgcacaggct catgaacgca tggcccgcga ggtggagaaa acccgccagc
    34741 gcatggaggc gctgagccag aaaatgcgcg aggaacaggc gcaggccatg gctctggcgg
    34801 aggctcagga taaagcagct gctgcgtttt atcgtcagat tgacagtgtg aaacaggcca
    34861 gtgcggggct gcaggaatta cagcgtattc agcagcagat ccgacaggcc agaaacagtg
    34921 gcgggattgg tcagcaggat tatctggcgc tgatttctga ggttacggcg aaaacccgtg
    34981 ttcttacgca ggctgaggaa gagtctaccc gacagaaagt ggcgtttatc cgtcagctta
    35041 aagagcaggc aacccgccag aatctttctt cttctgagtt gcttcgtgcc agggctgccc
    35101 agctgggggt aagcagtgct gcagaagtgt atatccgcaa aatggagcag gcaggaaaag
    35161 ccacgcattc gctgggtctg aaaagtgcag cagcccgcca ggagataggc gttctgatag
    35221 gtgaactggc ccgtggcaat ttaggagcgc tgaggggatc cgggataacg ctggctaacc
    35281 gtgccggatg gatagacaca ctgatgtcac cgaaaggcat gatgctgggc ggggttattg
    35341 gcggtattgc cgcggccgtc tatggtctgg gtaaagcctg gtatgacggt cagaaggagg
    35401 gggaagaatt taaccgccag ctgtcactga cggggcatta tgccggagtc actgccgggc
    35461 agctgtggac gctcagtcgt gctatttccg ggaatggtat cacgcaacat gctgcagccg
    35521 gtgcgctggc tcaggtggtg gggagtggtg catttcgtgg aaacgatatc ggtatggtgg
    35581 cgagagctgc cgcacagatg gagcgatcgg ttggccagtc ggtcagcgat accataaatc
    35641 agtttaagcg gctgaaggat gatcctgtaa atgccgcgaa ggctctggac aatgagctgc
    35701 attttcttac tgccactcag cttgagcaga tacgcgtcct tggggatcag gggcggtcca
    35761 gtgatgctgc acggatagcc atgtctgcac tggcagagga aaccggtcgg cgtactgcgg
    35821 atattgataa taacctcaat gcgctgggca gtacgctgaa gtatctgtct gatttatgga
    35881 gtcgtttctg ggatgcggcc atgaatattg gtcgtgaaga ctcgctggat gaacagattg
    35941 ccgctttaca ggagaaagtg tcgcgggcga aaagactccc ctggacggca tcatcttctc
    36001 aggttgaata cgatcagcag cgtcttaacg atcttcagga gaaaaaacgc cagaaggatt
    36061 tgcaggatgc aaaagagcag gcagagcgga attatcagga gcaacagaaa cgccgtaatg
    36121 ctgaaaatgc tgcactgaac cggatgaatg aaacggaagc tgcacgacat cagcgtgaaa
    36181 ttgcgcgtat taatgccatg cagtacgccg atcaggctgt cagggatgcg gcgatacaac
    36241 gtgaaaatga acgttacgag aaagccctgg catccggtaa gaaaaaaaca cgcgaaaccc
    36301 gtaatgatga ggccacccgg ttattgctgc agtacagtca gcaacaggca caggtggaag
    36361 gacagattgc tgctgccaga cagtcagcag gcattgccac ggaaaggatg acagaagcgc
    36421 ataaacagct tctggctctg cagcagcgca tcagcgacct ggacgggaaa aaactgacgg
    36481 cagatgaaaa gagtgtgctg gcccgtaaag atgaactgat tcaggcactg acgctgctgg
    36541 atgtaaaaca gcaggagctt cagaaacaga cggcactcaa cgagctgaag aaaaaaacaa
    36601 ttcagctgac cagtcaactg gctgaagaag agcgcgctca gcgtcagcaa catgacctgg
    36661 atatcgccac ggtgggtatg ggtgatcagc agcgacagcg atatcaggta caactgagtc
    36721 ttcgccagaa ataccagcaa cagctggagc agttgaggcg ggatagtgag cagaaaggga
    36781 catataacac ggatgactac agaaaggccg agcaggcgct gacggagagc ctgaaccgac
    36841 aactgaatga gaatcgccgt tactggcaac agcttgaagt tgtgcagggt aactggaaaa
    36901 acggagtcct gcgtgcattt caggatttta ccgtggatgc agataatacg gcagaaacag
    36961 cagaacaggt gttctcgtca gccttcagca acatgggaaa tggcctggca acttttgtca
    37021 ctaccggcaa actcaatttc aaatccttca cctcttctgt gctgtcagat atggcgaaaa
    37081 tcctggcgca ggcaaccatg atgaaatcga taaaagggat tggcagtgta ctgggatttg
    37141 atctcagcag cctttccctg aatgccaatg gggggattta tcagtctgct gatttgagtc
    37201 gttacagtgg cacggtggtt aaccgtccga cgttttttgc ttttgcaaaa ggcgcgggtg
    37261 tgatggggga agcgggacct gaagccattc tgccattgcg tcgtggtgct gacggtaagc
    37321 tgggggttgt ggcggatatt gggggttcag gtatggcgat gttttccccg cagtacaaca
    37381 tcgagatcaa taacgatggc acgaacgggc agataggtcc ggctgccctg aaggcggttt
    37441 atgacctcgg gaaaaaagcg gcagcggact ttatgcaaca gcaggcccgt gatggtggtc
    37501 ggttaagtgg agcatatcgg taatggagac gtttcactgg aaagtgcgcc cggatatgaa
    37561 tgtggtatca gagccgaaag tggtgacagt gaagctgggc gatggttatg aacagcgtcg
    37621 tgcggcggga ctgaataacc agttgtcgac ttacagcgtg acgatacgtg ttcgtaaatg
    37681 tgaacaccca tctttaaaag cctttctgga acggcacggt ggcgtccgtg catttcagtg
    37741 gacgccacct tatgactgga aaccgatcag ggtggtttgt cgtaaatggt cggcaagcgt
    37801 gggggcgctg tgggtaacca taacggcaga ttttgaacag gtcgtggcat aggaggctct
    37861 gatgcaggat attccacagg aaacacatca tgagacgaca cgcctcactc agtcagccca
    37921 ggtggtgctc tgggaaatcg atctgacaga ggtcggtggt gaacgttatt ttttctgtaa
    37981 tgagcagaac gaaaaaggtg agccggttac ctggcagggg cggcagtatc aggcataccc
    38041 cattcagggg acaggatttg aactgaacgg caagggcagt gctgcccgtc cgacactgac
    38101 ggtttctaac ctgcacggta tggtcaccgg gatggcggaa gatctgcaga gtctggtcgg
    38161 cggaacggtg gtccggcgta aggtttacgc ccgttttctg gatgcggtga acttcgtcaa
    38221 cggaaacagt gacgccgacc cggagcagga ggtgatcagc cgctggcgca tcgagcagtg
    38281 cagcgaactg agcgcggtca gtgcctcttt tgtactggcc acgccgacgg aaacggacgg
    38341 cgctgttttt ccgggacgta tcatgctggc caacacctgc acctggacct atcgcggcga
    38401 tgagtgcggt tatcacggtc cggcggtcgc ggatgaatat gaccagccaa cgtccgatat
    38461 cacgaaggat aaatgcagca aatgcctgag cggttgtaag ttccgcaata acgtcggcaa
    38521 ctttggcggc ttcctttcca ttaacaaact ttcgcagtaa atcccatgac agaaacagaa
    38581 tcagcgattc tggcgcacgc ccggcgatgt gcgccagcgg agtcgtgcgg cttcgtggtg
    38641 agaacgccgg agggagaaag atattttccc tgcgtgaata tctccggtga gccggaggag
    38701 tatttccgga tgtcgccgga ggactggctg cgggcagaaa tgcagggtga gattgtggcg
    38761 ctggtccaca gccaccccgg tggtctgccc tggctgagtg aggctgaccg gcggctgcag
    38821 gtgcagagtg atttgccgtg gtggctggtc tgccggggtg agattcataa attccgctgt
    38881 gtgccgcatc tcaccgggcg gcgctttgag cacggggtga cggactgtta cacgctgttc
    38941 cgggatgctt accatctggc ggggattgag atgccggatt ttcatcgcgg ggatgactgg
    39001 tggcgtaacg gtcagaatct ctatctggat aatctggagg ccacagggct gtatcaggtg
    39061 ccgttgtcag cggcgcagcc gggcgatgtg ctgctgtgct gttttggttc atcggtgccg
    39121 aatcatgccg cta
//
LOCUS       DAJTVJ010000028        38338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000028 DAJTVJ010000000
VERSION     DAJTVJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.028"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            60..1424
                     /locus_tag="OO448_001373"
     CDS             60..1424
                     /locus_tag="OO448_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6336625.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1552..2043)
                     /gene="yajQ"
                     /locus_tag="OO448_001374"
     CDS             complement(1552..2043)
                     /gene="yajQ"
                     /locus_tag="OO448_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6336626.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2211..3122
                     /gene="panE"
                     /locus_tag="OO448_001375"
     CDS             2211..3122
                     /gene="panE"
                     /locus_tag="OO448_001375"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6336627.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3085..3675
                     /gene="yajL"
                     /locus_tag="OO448_001376"
     CDS             3085..3675
                     /gene="yajL"
                     /locus_tag="OO448_001376"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6336628.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3729..5177)
                     /gene="thiI"
                     /locus_tag="OO448_001377"
     CDS             complement(3729..5177)
                     /gene="thiI"
                     /locus_tag="OO448_001377"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6336629.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5383..5625
                     /gene="xseB"
                     /locus_tag="OO448_001378"
     CDS             5383..5625
                     /gene="xseB"
                     /locus_tag="OO448_001378"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6336630.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5625..6524
                     /gene="ispA"
                     /locus_tag="OO448_001379"
     CDS             5625..6524
                     /gene="ispA"
                     /locus_tag="OO448_001379"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6336631.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6549..8411
                     /gene="dxs"
                     /locus_tag="OO448_001380"
     CDS             6549..8411
                     /gene="dxs"
                     /locus_tag="OO448_001380"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6336632.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8466..9440
                     /gene="yajO"
                     /locus_tag="OO448_001381"
     CDS             8466..9440
                     /gene="yajO"
                     /locus_tag="OO448_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6336633.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9494..10012)
                     /gene="pgpA"
                     /locus_tag="OO448_001382"
     CDS             complement(9494..10012)
                     /gene="pgpA"
                     /locus_tag="OO448_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6336634.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9990..10967)
                     /gene="thiL"
                     /locus_tag="OO448_001383"
     CDS             complement(9990..10967)
                     /gene="thiL"
                     /locus_tag="OO448_001383"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6336635.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11045..11464)
                     /gene="nusB"
                     /locus_tag="OO448_001384"
     CDS             complement(11045..11464)
                     /gene="nusB"
                     /locus_tag="OO448_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6336636.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11484..11954)
                     /gene="ribE"
                     /locus_tag="OO448_001385"
     CDS             complement(11484..11954)
                     /gene="ribE"
                     /locus_tag="OO448_001385"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6336637.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12043..13146)
                     /gene="ribD"
                     /locus_tag="OO448_001386"
     CDS             complement(12043..13146)
                     /gene="ribD"
                     /locus_tag="OO448_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6336638.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13150..13599)
                     /gene="nrdR"
                     /locus_tag="OO448_001387"
     CDS             complement(13150..13599)
                     /gene="nrdR"
                     /locus_tag="OO448_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6336639.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13750..14289
                     /locus_tag="OO448_001388"
     CDS             13750..14289
                     /locus_tag="OO448_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6336640.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14588..15472
                     /gene="tsx"
                     /locus_tag="OO448_001389"
     CDS             14588..15472
                     /gene="tsx"
                     /locus_tag="OO448_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6336641.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15649..15996)
                     /locus_tag="OO448_001390"
     CDS             complement(15649..15996)
                     /locus_tag="OO448_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6336642.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16125..17096)
                     /gene="secF"
                     /locus_tag="OO448_001391"
     CDS             complement(16125..17096)
                     /gene="secF"
                     /locus_tag="OO448_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6336643.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17107..18954)
                     /gene="secD"
                     /locus_tag="OO448_001392"
     CDS             complement(17107..18954)
                     /gene="secD"
                     /locus_tag="OO448_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6336644.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18982..19314)
                     /gene="yajC"
                     /locus_tag="OO448_001393"
     CDS             complement(18982..19314)
                     /gene="yajC"
                     /locus_tag="OO448_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6336645.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19337..20464)
                     /gene="tgt"
                     /locus_tag="OO448_001394"
     CDS             complement(19337..20464)
                     /gene="tgt"
                     /locus_tag="OO448_001394"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6336646.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20520..21590)
                     /gene="queA"
                     /locus_tag="OO448_001395"
     CDS             complement(20520..21590)
                     /gene="queA"
                     /locus_tag="OO448_001395"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6336647.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21683..22264
                     /gene="acpH"
                     /locus_tag="OO448_001396"
     CDS             21683..22264
                     /gene="acpH"
                     /locus_tag="OO448_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR6336648.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22269..24086)
                     /gene="malZ"
                     /locus_tag="OO448_001397"
     CDS             complement(22269..24086)
                     /gene="malZ"
                     /locus_tag="OO448_001397"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6336649.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24242..25615)
                     /gene="proY"
                     /locus_tag="OO448_001398"
     CDS             complement(24242..25615)
                     /gene="proY"
                     /locus_tag="OO448_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6336650.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25691..27010)
                     /gene="brnQ"
                     /locus_tag="OO448_001399"
     CDS             complement(25691..27010)
                     /gene="brnQ"
                     /locus_tag="OO448_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6336651.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27417..28712)
                     /gene="phoR"
                     /locus_tag="OO448_001400"
     CDS             complement(27417..28712)
                     /gene="phoR"
                     /locus_tag="OO448_001400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6336652.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28770..29459)
                     /gene="phoB"
                     /locus_tag="OO448_001401"
     CDS             complement(28770..29459)
                     /gene="phoB"
                     /locus_tag="OO448_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6336653.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDCAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29649..30851
                     /gene="sbcD"
                     /locus_tag="OO448_001402"
     CDS             29649..30851
                     /gene="sbcD"
                     /locus_tag="OO448_001402"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6336654.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30848..33991
                     /gene="sbcC"
                     /locus_tag="OO448_001403"
     CDS             30848..33991
                     /gene="sbcC"
                     /locus_tag="OO448_001403"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6336655.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34117..35301
                     /gene="araJ"
                     /locus_tag="OO448_001404"
     CDS             34117..35301
                     /gene="araJ"
                     /locus_tag="OO448_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6336656.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35444..36352)
                     /gene="mak"
                     /locus_tag="OO448_001405"
     CDS             complement(35444..36352)
                     /gene="mak"
                     /locus_tag="OO448_001405"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6336657.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36477..37388
                     /gene="rdgC"
                     /locus_tag="OO448_001406"
     CDS             36477..37388
                     /gene="rdgC"
                     /locus_tag="OO448_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6336658.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37466..37921)
                     /locus_tag="OO448_001407"
     CDS             complement(37466..37921)
                     /locus_tag="OO448_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336659.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37918..38253)
                     /locus_tag="OO448_001408"
                     /pseudo
     CDS             complement(37918..38253)
                     /locus_tag="OO448_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9474 a        10315 c         9727 g         8822 t
ORIGIN      
        1 ctgtttaccc aacatgcgct ccgcactaca ctatgtgggc atttttttaa ggggtggtaa
       61 tgaacgatta taaaatgacg ccaggtgaga ggcgcgcgac ctggggttta gggaccgtat
      121 tctcgttgcg catgctgggc atgtttatgg ttctgccggt tctgaccacg tacggcatgg
      181 ctctgcaagg tgccagcgaa gcattaatcg gtattgccat tggtatttat ggtctgactc
      241 aggccgtttt tcagattccg tttggcctgc tttccgaccg cattggtcgc aaaccattaa
      301 ttgtcggtgg gctggcggtg tttgccgccg gtagcgttat cgctgcgctt tccgactcca
      361 tctggggaat tattctgggc cgggcgctac aaggctctgg agcaattgcc gccgccgtta
      421 tggcgctgct ttccgatctc acgcgcgaac aaaaccgcac caaagcgatg gcgtttatcg
      481 gcgtgagctt tggcattacc tttgccattg cgatggtgct tggcccgatc atcactcaca
      541 aacttgggct gcacgcgctg ttctggatga tcgctattct ggcaacaacc ggcattgcgt
      601 tgaccatttg ggttgtgccc aacagtagca ctcacgtact taatcgtgag tccggaatgg
      661 tgaaaggcag tttcagtaaa gtgctggcgg aaccgcggct gctgaaactc aactttggca
      721 ttatgtgtct gcacatgctt ctgatgtcta cgtttgttgc cctgcccgga cagctagctg
      781 atgcggggtt cccggcggct gaacactgga aggtctatct ggcgacgatg ttaatcgcct
      841 ttggctcgat cgtgcctttc attatctacg ctgaagttaa gcgcaaaatg aagcaagtct
      901 ttgtcttctg cgtcgggttg atcgtggttg cggaaattgt gttgtggaac gcacaaacgc
      961 agttctggca actggtggtc ggcgtgcagc ttttctttgt ggcgtttaat ttgatggaag
     1021 cccttctgcc ctcacttatc agtaaagagt cgccagcagg ttacaaaggt acggcgatgg
     1081 gtgtctactc caccagccag tttcttggcg tggcgattgg cggttcgctg ggcggctgga
     1141 ttgacggcat gtttgacggt cagggggtat ttctcgctgg cgcaatgctg gccgcagtgt
     1201 ggctggcagt cgccagtacc atgaaagaac cgccgtatgt cagcagtttg cgcattgaaa
     1261 tcccggcgga cattgccgca aacgaagcgt taaaagtgcg tttgctggaa actgaaggcg
     1321 tcaaagaagt gttgattgca gaagaagagc attcagctta tgtgaaaatc gacagcaaag
     1381 tgacaaatcg ctttgaggtt gaacaggcaa ttcgtcaggc ataaaaaacg cggcgaattg
     1441 cctgctgcgc tacgcttacc aggcctacaa agttcttgag ttacaaaccg ttgtaggccg
     1501 aatcaggcgt tcatgccgca tctgacatga acaaaacgca cattgtcgcg attaatcgcg
     1561 gaagtttttg aactggaacg gctggccgag atcgccacca cgtaccatcg ccatgacagc
     1621 ctgcaaatca tcacgagatt tgccagtcac acggatctca tcgccctgaa tttgcgcctg
     1681 cactttcagt ttgctgtctt taatcatctt gacgattttc ttctgggtcg cgctctcaat
     1741 gccctgtttc agtttcgctt ccacaaacca ggttttaccg ctatgaacga tattttccgg
     1801 tacatccagt gaactgcctt caatgccgcg cttcagcagc ttggcacgca gaatatccag
     1861 caactgattg acctggaaat cggactcgct caacactttg atggttttgc tggcgtcgtt
     1921 cagctcaaat gaggcttcaa cattacggaa gtcaaaacgg gactccactt cgcggctcgc
     1981 gttatcgacc gcgttacgtg cttcctgaag atcaacttca gagacaatat cgaaagatgg
     2041 catcttttcc tctcccttca tttttgatgc gaagcataat acccgcaaag ttaaataacc
     2101 gacagtcctg caccaggacg tatcccttaa ttgtgcatag tcacaacagc atgcacaatt
     2161 aagggatagc cttattgaac acccggagtg gttgcgggtg aggaggaaca atgaaaatta
     2221 ccgtattggg atgcggtgcc ttagggcaat tatggcttac agcactttgt aaacagggtc
     2281 acgaagttca gggctggctg cgcgtaccgc aaccttattg tagtgtgaat ctggttgaga
     2341 ctgatggttc gatatttaac gaatcgctga ccgccaacga tcccgatttt cttgccacca
     2401 gcgatctgct cctggtgacg ctgaaagcat ggcaggtttc cgatgccgtc aaaagcctcg
     2461 cgtccacact gcctgtaacc acgccaatac tgttaattca caacggcatg ggcaccatcg
     2521 aagagttgca aaacattcag cagccattac tgatgggcac caccacccat gccgcccgcc
     2581 gcgacggcaa tgtcattatt catgtggcaa acggaatcac gcatattggt ccggcacggc
     2641 aacaggacgg cgattacagc tatctggcgg atattttgca aaccgtgttg cctgacgttg
     2701 cctggcataa caatattcgc gccgagctgt ggcgcaagct ggccgtcaac tgtgtgatta
     2761 atccactgac cgccatctgg aattgcccga atggtgaatt acgtcatcat ccgcaagaaa
     2821 ttatgcagat atgcgaagaa gtcgcggcag tgatcgagcg cgaagggcat catacttcag
     2881 cagaagattt gcgtgattac gtgatgcagg tgattgatgc cacagcggaa aatatctcgt
     2941 cgatgttgca ggatatccgc gcgctgcgcc acaccgaaat cgactatatc aatggttttc
     3001 tcttacgccg cgcccgcgcg catgggattg ccgtaccgga aaacacccgc ctgtttgaaa
     3061 tggtaaaaag aaaggagagt gaatatgagc gcatcggcac tggtttgcct cgcccctggt
     3121 agtgaagaga ctgaagccgt caccactatc gatctgctgg ttcgcggcgg tatcaaagtc
     3181 accactgcca gcgtcgccag cgatggcaac ctggccatta cctgctcgcg cggcgtgaag
     3241 ctgctggcgg atgcgccgct ggtcgaggtg gctgatggcg aatatgacgt gatcgtactg
     3301 cctggtggca ttaaaggcgc agagtgtttt cgcgatagta ccctgctggt tgaaaccgtt
     3361 aaacagttcc accgttccgg gcgtatcgtc gcggctattt gcgccgcgcc agccaccgtg
     3421 ctggtaccgc acgatatctt cccgattggc aatatgaccg gcttcccgac gctgaaagac
     3481 aaaattcccg ccgaacaatg gcaggacaag cgcgtcgtct gggatgcacg ggtaaaattg
     3541 ctgaccagcc aggggccggg tacggctatt gattttggtc tgaaaattat cgacctgctg
     3601 gttgggcgcg agaaaaccca tgaagtggca tcacaactgg tgatggcggc agggatttat
     3661 aattattacg agtagtgtcg gatgcggcaa acgtcgcatc tgaccagatg cgacgtaaaa
     3721 accacaaatt atgggcgata caccttcaca ttgttaaagc cctgctcgcg cagatagagc
     3781 gcctgcagac ggctcatcac cccgcgctca caccacagta gccaggttct gttctggtcg
     3841 agatcgccaa atttggtgct cagtttatag aacggcagag aaaccacgtc gataccttca
     3901 accttcagcg gcttatcttc ctgctcatcg acagaacgga tatcgaggat cacgtcgttc
     3961 gggccaaagc cattgacggt ttccacttcc accacttcct gctcggtctg ctgggcgatt
     4021 tcgcggatat caacgttatt cgcttcctca accactttat cgagaatact gaagtcgaac
     4081 ttctcttctt ccgcttcaat cttcgattta accgctttca ccgtcgggct tttggagatc
     4141 acgccacaat attctggcat cgtgcgagca aagtcttcag tgccaatctg gcgggccagg
     4201 ttgatgatgt gctctttgtc gtaagaaatc agcggacgca ggatcagcgt atcagagacg
     4261 ttatcaatca ggcgcaggtt ggtcagcgtc tgactggaca cctggccgag cgcttcgccg
     4321 gtgaccagcg cctgtacgcc gtaacgttca gccactttag acgcggcacg caccatcata
     4381 cgtttgagga taacgcccat ctgaccgtcg tcgattttct cgagaatttc cccgacgacc
     4441 ggttcgaaat taatggcgac aaaacgcacg cggtgggagc tgccaaagcg gttccacaga
     4501 taatgcgcca cctgacgcac gccaatttca tgcgctgcgc cgccgaggtt aaagaagcag
     4561 taatgcacgc ggcagccgcg acgcatcaac atataactgg aaacaccgga gtcgaaacca
     4621 ccggaaatga gcgacagcac atcttcctgg gtgccaatcg ggaaaccgcc aataccttcg
     4681 tagcggcctt taatcagcag aagacgatcg tcttccactt ccagatggac agttacatcc
     4741 gggttagtca gcttcacgcg cgcggattca atatgctgat ttaaaccgcc gccgacgtaa
     4801 cgttccacat caatcgagct aaaatcatgt ttgccacggc gcttcacgcg tacgcagaag
     4861 gttttgcctt ccagctgatc gcgatattga accaacgctt tctcgaaaat atcgtgcatg
     4921 tcggtaaacg gcacgtcttc gacttcgaga atatggtgga tacccggaat acgggtcagg
     4981 gcgtcgcgaa tagccagacg ctgtttttca tcttttgcgc gaacttcgat gttatcccag
     5041 tggcggacga cagcgagcgt ctcatcatag tgctttaaaa cgttacgaat gttcccggta
     5101 aggattttta taaagcgcaa gcgcacagat tggcttttga tggtgatttc cgggaacaat
     5161 ttaatgataa acttcatggc ggcaatggtt cgttggcaag ccttaagcga cttgtatagg
     5221 gaaaaataca gcagcccaca cctgcggctg catccaggcg cggaagtata ccactaacat
     5281 cgctttgctg cgcacatcac cttaccattg cgcgttattt gctatttgcc ctgagtccgt
     5341 taccatgacg gggcgaaaaa tattgagagt cagacattca ttatgccgaa gaaaaatgag
     5401 gcgcccgcca gctttgaaaa ggcgctgagc gagctggaac agattgtaac ccgtctggaa
     5461 agtggcgacc tgccgctgga agaggcgctg aacgagttcg aacgcggcgt gcagctggca
     5521 cgtcaggggc aggccaaatt acaacaagcc gaacagcgcg tacaaattct gctgtctgac
     5581 aatgaagaca cctctctacc cccttttaca ccggacaatg agtaatggac tttccgcagc
     5641 aacttgaagc ctgcgttaag caggccaacc aggcgctgag ccgttttatc gccccactgc
     5701 cctttcagaa cactcccgtg gtcgaaacca tgcagtatgg cgcattatta ggtggtaagc
     5761 gcctgcgacc ttttctggtt tatgccaccg gtcatatgtt cggcgttagc acaaacacgc
     5821 tggacgcacc cgctgccgcc gttgagtgta tccacgctta ctcattaatt catgatgatt
     5881 taccggcgat ggatgatgac gatctgcgtc gcggtttgcc gacctgccat gtgaagtttg
     5941 gcgaagcaaa cgcgattctc gctggcgacg ctttacaaac gctggcgttc tcgattttaa
     6001 gcgatgccga tatgccggaa gtgtcggacc gcgacagaat ttcgatgatt tcagaactgg
     6061 caagcgccag cggtattgcc ggaatgtgcg gtggtcaggc attagattta gatgcggaag
     6121 gcaaacacgt acctctggac gcactcgagc gtattcatcg tcataaaacc ggcgcattga
     6181 ttcgcgccgc cgttcgcctt ggtgcattaa gcgccggaga taaagggcgt cgtgctctgc
     6241 cagtactcga caagtacgca gagagcatcg gccttgcctt ccaggttcag gatgacatcc
     6301 tggatgtggt gggagatact gcaacgttgg gaaaacgcca gggtgccgac cagcaacttg
     6361 gtaaaagtac ctaccctgca cttctgggtc ttgagcaagc ccggaagaaa gcccgggatc
     6421 tgatcgacga tgcccgccag tcgctgaaac aactggctga acaatcactc gatacctcgg
     6481 cactggaagc gctagcggac tacatcatcc agcgtaataa ataaacaata agtattaata
     6541 ggcccctgat gagttttgat attgccaaat acccgaccct ggcactggtc gactccaccc
     6601 aggagttacg actgttgccg aaagagagtt taccgaaact ctgcgacgaa ctgcgccgct
     6661 atttactcga cagcgtgagc cgttccagcg ggcacttcgc ctccgggctg ggcacggtcg
     6721 aactgaccgt ggcgctgcac tatgtctaca acaccccgtt tgaccaattg atttgggatg
     6781 tggggcatca ggcttatccg cataaaattt tgaccggacg ccgcgacaaa atcggcacca
     6841 tccgtcagaa aggcggtctg catccgttcc cgtggcgcgg cgaaagcgaa tatgacgtat
     6901 taagcgtcgg gcattcatca acctccatca gtgccggaat tggtattgcg gttactgccg
     6961 agaaagaagg caaaaatcgc cgcaccgtct gtgtcattgg cgatggcgcg attaccgctg
     7021 gcatggcgtt tgaagcgatg aatcacgcgg gcgatatccg tcctgatatg ctggtggtcc
     7081 tcaacgacaa tgaaatgtcg atttccgaaa atgtcggcgc gctcaataac catctggcac
     7141 agctgctttc cggtaagctt tactcttcgc tgcgcgaagg cgggaaaaaa gttttctctg
     7201 gcgttccgcc aattaaagag ctgctcaaac gtaccgaaga acatattaaa ggcatggtag
     7261 tgcctggcac gttgtttgaa gagctgggct ttaactacat cggcccggtt gacggtcacg
     7321 atgtgctggg gcttatcacc acgctgaaga acatgcgcga cctgaaaggc ccgcagttcc
     7381 tgcatatcat gaccaaaaaa ggtcgtggtt atgaaccggc agaaaaagac cccatcactt
     7441 tccacgccgt gcctaaattt gatccctcca gcggttgttt gccgaaaagt agcggcggtt
     7501 tgccgagcta ttcaaaaatc tttggcgact ggttgtgcga aacggcagcg aaagacaaca
     7561 agctgatggc gattactccg gcgatgcgtg aaggttccgg catggtcgag ttttcacgta
     7621 aattcccgga tcgttacttc gacgtggcaa tcgccgagca acacgcggtg acctttgccg
     7681 ccggtctggc gattggtggg tacaaaccca ttgtcgcgat ttactccact ttcctgcaac
     7741 gcgcctatga tcaggtgctg catgacgtgg cgattcaaaa gctcccggtc ctgttcgcca
     7801 tcgaccgcgc gggcattgtt ggtgctgacg gtcaaaccca tcagggcgct tttgacctct
     7861 cttacctgcg ctgcataccg gaaatggtca ttatgacccc gagcgatgaa aacgaatgtc
     7921 gccagatgct ctataccggc tatcactata acgacggccc gtccgcggtg cgctacccgc
     7981 gcggtaacgc ggttggcgtg gaactgacgc cgctggaaaa actgccaatt ggcaaaggca
     8041 ttgtgaagcg tcgtggcgag aaactggcga tccttaactt tggtacgctg atgccagacg
     8101 cggcgaaagt cgctgaatcg ctgaacgcta cgctggtcga tatgcgtttt gtgaaaccgc
     8161 ttgatgaagc gttaattctg gaaatggccg ccagccatga agcgctggtc accgtagaag
     8221 aaaacgccat tatgggcggc gcaggcagcg gcgtgaacga agtgctaatg gcccatcgta
     8281 aaccagtacc cgtgctgaac attggcctgc ctgacttctt tattccacaa ggaactcagg
     8341 aagaaatgcg cgccgaactc ggcctcgatg ccgccggtat ggaagccaaa atcaaggcct
     8401 ggctggcata atccctactc cactcctgct atgcttaaga aattattcat ggcaggagtg
     8461 gcagcatgca atacaacccc ttaggaaaaa ccgaccttcg cgtttcccga ctttgcctcg
     8521 gctgtatgac ctttggcgag ccagatcgtg gtaatcacgc atggacacta ccggaagaga
     8581 gtagccgtcc catcattaaa cgcgcgctgg aaggcggcat aaatttcttt gataccgcca
     8641 atagctattc cgatggcagc agcgaagaga tcgttggtcg cgcactgcgg gatttcgccc
     8701 gtcgtgaaga cgtggtcgtt gcgacgaaag tgttccatcg cgttggtgat ttaccggaag
     8761 gattatcccg tgcacaaatt ttgcgctcta tcaacgacag cctgcgccgt ctcggcatgg
     8821 attatgtcga tatcctgcaa attcatcgct gggattacaa cacgccgatc gaagagacgc
     8881 tggaagccct gaacgacgtg gtaaaagccg ggaaagcgcg ttatatcggc gcgtcatcca
     8941 tgcacgcttc gcagtttgct caggccctga agctacaaaa acagcacggc tgggcgcagt
     9001 ttatcagtat gcaggatcac tacaatctga tttatcgcga agaagagcgc gagatgctgc
     9061 ctctgtgtta tcaggagggc gtggcggtga ttccgtggag cccgctggcg cgggggcgac
     9121 tgacgcgtcc gtggggagaa actaccgcac gactggtgtc tgatgaggtg gggaaaaatc
     9181 tctataaaga aagcgatgaa aatgacgcgc agatcgcaga gcggttaacg ggcgtcagtg
     9241 aagaactcgg tgcaacacga gcacaagttg cgctggcctg gttattgagt aaaccgggca
     9301 ttgccgcacc gattatcggt acatcgcggg aagaacagct tgatgagctg ctgaacgcgg
     9361 tggatatcac tttgaagcca gaacagattg ccgaactgga aacgccgtat aaaccgcatg
     9421 cggtcgtagg atttaaataa gagtccaggc ctgatgagac gtgacaagcg tcacatcagg
     9481 catcggtgca caactacgac agaataccca gcggccagtg atgaccgata aagtacagga
     9541 tgcctgcgga aatcaccccg gcgacaatat cgtcgatcat gatccccatg ccgccatgta
     9601 cattgcgatc aaaccagcgg atcggccacg gcttccacat atccagaata cggaaaatca
     9661 caaacccggc ggcaacccac tgccagtcat tggtcggcag cgccatgagc gtgatccaca
     9721 taccaataaa ttcgtcccag acaatgctgc cgtgatcgtg cacgcccata tctttcgccg
     9781 tttgatgaca aagatagacg ccgatacaga tccccaacat caccaccagc gagtagagct
     9841 gccagggtaa aaaggtcatc agataccaga acggaatcgc tgccagcgat cccatcgtgc
     9901 caggaacgat tgggcttaat ccactcccga atccgacagc aagcagatgc cacggattac
     9961 tcatcttcag gcgacttttc gcgacatctt tatggcgtgg caaaatggtc atatcctttc
    10021 cagtcaaacg tgacaggttc gccgtcacga ataaaacaaa gcccttcgat atcggcggtc
    10081 atttgcccga tacaggtaaa cggtacgcca aggtgtccga gagcaacatc cagcgcgcca
    10141 cggttcagtt ccggcaccgt gaaacacaac tcgtaatctt caccgccaga gagcgcccag
    10201 cgcagcgcct gttccggttc aacatggcga gaaagcgcat cagaaaacgg cagcaatgcc
    10261 aggtcaatac gtgcgccgca gtcgctggct ttcacgatat gcccgagatc ggaaatcaga
    10321 ccgtcagaga gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata
    10381 cgcggcgatg gacggagatg acgtttgatc aagtagtcag catctttagc atcggcaacc
    10441 tgcaaacggt tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt accggtcaca
    10501 tagatccagt cacccggttt cgccccagag cgcgttaagg ctcgtcccat cggaacaaag
    10561 ccgtggatac ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc
    10621 atatcgtaat aattgagaag atcaaacaaa ctgtcgctga aggactcaag ccacgcttcg
    10681 tctacgtccg gtaaggttaa tgccagcgtc agccaggccg gatcggcccc catcgctgcc
    10741 agatcgctta ggttcaccgc cagtgcttta taagccagat cagcaggatc gatatcaggg
    10801 aggaaatggt tacccgccac cagcgtatca gtgctgatcg ccagggtctg tttctcgggg
    10861 atattgagaa gtgcgcaatc gtcgccgatg cccagttcga catcaagacg agaacttctt
    10921 acacggtcaa aataacgggc aatcagggag aactcgccac atgccatacg ttatgcctca
    10981 gcaggtaaaa aagaaaaggc cgacgatcat ggaacggtct tccgtgaatc taccggcctg
    11041 gatatcactt tttgttaggg cgaatcacag gtgctgcttt atcgagtacg ccgttgacga
    11101 acttatggct gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca
    11161 ctttgtatgg cacatcgcta cgtttagaca gttcgtacag cgcaatgcgc agtactgctt
    11221 tttctacctg tcccagttct tccagcaggc gggacaggta tggcttcatc agtccgtcga
    11281 ggtatgcggt attagtcgcc accccggcca gcagctcacg gaagtacagg acgtcaacgt
    11341 cttttacatc ctgttcagcc aggaactggt attcaacatc agcgatgtcg ttctgggaca
    11401 actgccagga gtagagcgcc tggacggcac actcacgagc gcggcgacga gcagcaggtt
    11461 tcacggattt ccccttacta atttcaggcc ttgatggctt tcaatacatt aatcatttca
    11521 agcgcggtca gtgcagcttc tgcacctttg ttgccagctt tggtgccagc acgttcgatc
    11581 gcttgttcaa tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg
    11641 gcaacatgcg ccaggccgtt gcttgcacca ccagcgacat attcaaagtg ggcagtgcca
    11701 ccacgaataa ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt agccagtgca
    11761 cccgccgcca gcggcagctc ataggcacca ggcacccaaa caacggtaat gttttcatct
    11821 tttacctgac cgatacgttt cagtgcgtca attgcacctt ccagcaggct gtcattgata
    11881 aagttgttga aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc aacgttagct
    11941 tcaataatgt tcattttctt ccttcgggtt cgagtatggc cccgcagggg ggcggatttt
    12001 agcataatat ttcgtgcgct gcttcccttt cgagccggga gatcatgcac ccactaaatg
    12061 caggcaaaca tccgggccta catgacgtat ctctttgaat ttaaattggg gggcgtcggc
    12121 taatttctca agccctggca gcgtgcataa tccgcgggcg tcgctgccta atagtttagg
    12181 tgcgatatag acaatcagct catcgactaa acccgcctgc agcaatgcgc cagcgagcgt
    12241 tggccccgct tccacccaga tgctgttaat ttgctgttta cccagttgca tcatcagtac
    12301 aaccagatcc agatgacctt tatgctctgg aatcagcaag gtacgcaccg tttccggcca
    12361 ctcactagaa tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgcg
    12421 atgttccggc gtcacgcgat tttggctatc aatcacaata cgtactggct ggcggagatt
    12481 ttgttgcggg tagagcgcct gagtttgttc atccagttca gaccaacgca ctgttaaggc
    12541 tggatcatcc gccagcaccg tggcgctgct ggttaaaatg gcatgacttt gtgcgcgctg
    12601 tcgttgtacg tcgcgccgcg cctgaggcga agtgatccac tggctttcgc cgctcgccat
    12661 cgccgtgcgg ccatcaagcg atgcgccaag ttttaactga atataaggaa agccggtgcg
    12721 catccgcttg agaaagcctt tattcaattg ctcggcttca ctcatcatca gcccgtggct
    12781 gacgtcaatg ccagcctgtt gcagacggta aagtccacgc ccagcgacct gcgggttagg
    12841 atcctgcatc gctgcgacca cgcgcgctac gccagcggcg atgagtgcgt cacagcacgg
    12901 tggcgtacgg ccatgatggc tacagggttc gagtgtcaca tacgccgttg cgcctttggc
    12961 tttttcaccc gccatacgca acgcgtgtac ttcggcatgt ggttcacccg ctcgttggtg
    13021 gtaaccttca ccgacaattt cgccatcttt gacaatgacg cccccgacat tcgggttggg
    13081 gtgggtggta aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt aatactcgtc
    13141 ctgcacggct tagtcctcca ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa
    13201 actgcggtag acagaggcaa aacggatata ggcgacttta tcgagctttt tcaattgctc
    13261 catcaccaga ttgccaatca tcttgctcgg tacttcgcgc tcaccggtgg cgcgcagctg
    13321 cgatttaata tgattgattg ccatttcaac gtcatcggaa ctcaccggac gtttttccag
    13381 cgcccgcagc attccgctac gcaatttctc ttcattaaac ggttcacgca cgtcgttgct
    13441 ttttacaaca cgcggcataa ccagctccgc cacttcaaag gtggtgaaac gttcattaca
    13501 caccagacac tgccgacggc ggcgtacgga tgaaccctcg cccacgagac gagagtcaat
    13561 taccttagtg tccacggcga aacagaatgg gcaatgcata cggcgtcctg accaggtggt
    13621 taacggaaat ctattttacc ctgaactgac gatacaacaa aggcgcaacg cttttgagat
    13681 aaaggatcga tgctttcgct gcttttaccc tctaccatta ggctaattgc gacgagaaag
    13741 ggacgaccta tgaatactaa cgtttttcga ctgctcctgc tgggaagcct gttcagcctt
    13801 agcgcctgtg tgcagcaaag tgaagtgcga cagatgaaac acagcgtcag cacgctgaac
    13861 caggagatga cgcaactcaa tcaagaaaca gtcaaaatca ctcagcaaaa caggctgaat
    13921 gcaaaatcca gcagtggggt ttaccttctg cctggagcga aaacaccggc aagactggaa
    13981 agccagatcg gtactttacg tatgtcgctg gtgaatatta cgcctgatac agatggcacc
    14041 acccttacac tacgtattca gggtgagtcg aatgatccct tgcccgcatt cagcggaact
    14101 gttgaatatg ggcaaattca gggaacaata gacaactttc aggaaatcaa tgtgcagaat
    14161 caattgatta atgcccctgc cagcgtcctc gcccccagcg atgttgatat tccgttacaa
    14221 ttaaagggga tttctgtgga tcagttaggc tttgtacgta tccacgacat tcaacctgtt
    14281 atgcagtaaa cgtatttcgg gacgattttg tgcgtcccgc aacatctttc cccgtcattt
    14341 tgttactctg cttacatcac ctggattgat agtaaaagtt tgcaacaagg gcgaaagtca
    14401 gtacaatccc cgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa
    14461 ctgtgaaacg aaacatattt ttgtgagcaa tgatttttat aataggctcc tctgtatacg
    14521 aaatatttag aaacgcaatt tgcgcctttt tcactcccgc aagggatttt caaacagtgg
    14581 catacatatg aaaaaaacat tactggcagc cggtgcggta ctggcgctct cttcgtcttt
    14641 tactgtcaac gcagctgaaa acgacaaacc gcagtatctt tccgactggt ggcaccagag
    14701 cgttaacgtt gtcggaagct atcacacccg tttcggaccg cagatccgca acgataccta
    14761 ccttgagtac gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc
    14821 gccggtattc ttcggcggta actccgatgc taaaggtatc tggaaccacg gttctccgct
    14881 gtttatggaa atcgaaccac gtttctccat cgacaagctg accaatactg accttagctt
    14941 cggtccgttc aaagagtggt acttcgcgaa caactacatt tacgacatgg gtcgtaataa
    15001 agatggtcgc cagagcacct ggtacatggg tctgggtacc gacatcgata ctggtctgcc
    15061 gatgagcctg tccatgaacg tctatgcgaa ataccagtgg cagaactatg acgcagcgaa
    15121 cgaaaacgag tgggacggtt accgtttcaa aattaaatac tttgtgccga ttaccgatct
    15181 gtggggcggt cagctgagct acatcggctt caccaacttc gactggggtt ccgatttagg
    15241 ggatgacagc ggtaacgcaa tcaacggtat taagacccgt actaataact ctatcgcttc
    15301 cagccatatt ctggctctga actacgatca ctggcactac tctgtcgtag ctcgttactg
    15361 gcacgacggt ggtcagtgga acgacgatgc agaactgaac ttcggcaacg gcaacttcaa
    15421 cgttcgctct accggctggg gtggttacct ggtagtaggt tacaacttct gattatgaaa
    15481 atgccgggat ttattcccgg catttctgat tgttaacaac gtgtgctttg ttcatgccgg
    15541 atgcggcgta aacgctttgt ccggcccaca aaagcacgta aattcaatat attgctgaaa
    15601 taacgtaggc ctgataagcg tagcgcatca ggcaatttta cgttttaatc acttcttctt
    15661 gttcatcatc gctttcaggt cagcgaatgg gttgtacttc gcttcaccga catctttctg
    15721 cgcatcttcc cctgcgataa cggtcgtacc atactgatcc gcttcggtat atttcgaatg
    15781 ctcatgatcg tggcaataga gacaaagcaa ttcccagtta ctgccatctt ccgggttatt
    15841 ggtatggtcg tgatcaatgt ggtgaacggt aagttcacgc aggttggaat aaacaaactc
    15901 gcgggaacag cgaccgcaga cccacggata gatttttaat gctttttcgc gatagccgct
    15961 ttctaaccgc gcatagtttt ttgggatgat agccatgaaa agttacctgg atgaaatgta
    16021 gggttacgcg cagtttccca agatgcgggg attttcgcaa tctccataca tcaaaaaaaa
    16081 tcccgatctt ctgaccggga ttttcaacat caacgggaac ttgattacgg cagaattgac
    16141 ggctgatccg ccccttcttt ttccactttc tgctgcaaca tgtgttcgcg cttcataccc
    16201 agtttcagag ccaacgcaga tgctacgtag atggaagatg cagtgccgat ggaaacaccg
    16261 ataagcatgg tcagcgagaa gccttccagt accggaccac cgaagagata cagcatcagg
    16321 ataaccatca aggtagtacc ggatgtgatc aaggtacggt gcagcgtctg ggtcaaggac
    16381 acgttaaaga tttcgtaagg cgtaccgcga cggatcttgc ggaagttttc acgaattcgg
    16441 tccgatacca cgatactgtc gttaagcgag taaccgataa ccgacatcaa cgatgccaca
    16501 atggtcaggt caatctcgat atggaataac gacaaaatac ccagcgtaat gataacgtcg
    16561 tgcgccagcg caataaccac ccctgccgcc agtcgccact caaagcggaa acctacgtac
    16621 acgaggatag acagcagcgc tgccatcaac gccatcgcac cggtttgcgc aaggtctgcc
    16681 cccacgctcg gaccgacgaa ctcaatacgc ttcactgctg cattctgatt ggtggattcg
    16741 ttaatcacct tcagaacctg gctgcccagc acctgaccgc cggtttcgcc ttcagcaggc
    16801 ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt gcagcattgg ctcttcaaaa
    16861 ccggcttttt gcaatgcatc acgcattacg tcaatttcag ccggtttttc gagcgtaatt
    16921 tcgataaccg taccaccggt gaaatccagc ccccagttaa agccgcgcac gcccataata
    16981 acgatagcag cgattaacag cagaccagag atgccgaaag cccagtagtc ccagcgcata
    17041 aagtcataga ctttacggcc gtggtttagt tgttcaacag tatattcctg tgccacatcg
    17101 cactcctcag attgacagct tcttgacgcg cttgccgcca tatagcaggt ttacgatggc
    17161 acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca ccgataccgg tagtaatcgc
    17221 gaacccttta attgccccgg tacccactgc gtacaggatg atgactttaa tcagcgtggt
    17281 gatgttcgca tcgaagatag aactgaatgc gccacgataa ccttcatcaa ttgcctgttg
    17341 aacagtacgt ccgttgctca actcttcttt aatacgttcg ttgatcagta cgttcgcatc
    17401 gaccgctacc gcaagggtta agacgatacc cgcgatacct ggcatactca gtgttgcgcc
    17461 tggcaacagc gacataatac cgacgatcaa gatcaagttg gcaatcagag cactggtcgc
    17521 aatcagacca aacttcttat agaagatgat catgaacaga atagaaacca gcagaccggc
    17581 aaggcaagcc tccagcccct gctcaatgtt ctgcataccc agggtcgggc caattgtacg
    17641 ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca cgcagcagca gtgacagctg
    17701 acgggcttcg ttcgggttgt tgatgccggt gatacggaag ctgttaccca gacgagactg
    17761 gatgttggcg atgttaatca cctcttcctg tttcaccaga accgcacgac catttgcatc
    17821 tttcttaccg ctgtctttgt actccacaaa cagggttgcc atcggtttgc cgatgttgtc
    17881 cttggtgaag ttagacataa tgttgccacc agcgctatcg agcgagatgt taacctgcgg
    17941 ctggttgtat tcgtcctggc tggaagtgga gtcggtgata tggtcaccgg tcagaattac
    18001 gcgtttgtac agcacaaccg gctgaccttc gcgggtctgt ttcacttcag agtcgcccgg
    18061 tacgcgaccg gatgccgcag cggcctgatc aacattggtg tttaccagac ggaattccag
    18121 cgttgccgtc gcgcccagaa tctctttcgc acgcgcagtg tcctgaatac caggcagttc
    18181 aacaacgata cggtcagcac cctgacgctg aaccaccggc tccgccacgc caagttggtt
    18241 tacacggtta cgcaggatat taatgttctg ctgcaccgca tattcacgcg cttcactcag
    18301 acgagcatcg ctcattaccg cacgcagctg gttgctgccc tggctgctaa tcaccaggtc
    18361 cggatggcgc ttgctcagat acgcaatggc ttcatcacga gctttagcat cgcggaaagt
    18421 gatgctcagg ccgtagttgt tttctttacg aacagtggta tacgggatgc ccttttcgcg
    18481 caggtcactg cgtaggctat cgatattttg ttcctggagt ttgccaagcg cggtgtccat
    18541 atccacttcc atcaggaagt gaacgccgcc acgcaggtca aggccgagct tcatcggctc
    18601 agcatgaata gctgccagcc agcgcggcgt tgccggggca aggttaagcg ccacgacgta
    18661 tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc aactgggtgt cagtggagtc
    18721 gaagcgcgca agaatagcgc cctcttccag tgccacagac ttagcagtta ttttttcttc
    18781 ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg gcggcgactc cgcgcgcacc
    18841 agtgatctga acagccggat cctcaccaaa caggttggga agcgcataca gcagaccgat
    18901 gacaatcacc acgatcagca tgacgtactt ccacaaagga taacggttta acacggcaat
    18961 tcccttaggg aaaaatttta attacagcgc cttcatggtg cctttcggca ggacggcagc
    19021 tacgaagtca cgtttaataa ctacttcagt ggtgtcattc agcgcgatag caatgtagcc
    19081 gttttccgct actttggtta cgcgaccaac caggccaccg ttcgtcagaa cttcatcacc
    19141 tttggcaatg gagtccatca gctttttgtg ttctttggtg cgcttctgct gtggacgcag
    19201 gatcatgaaa tagaaaatca gaccgaatac caccagcatc aaaatcaaag acatcgggct
    19261 accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa ataaaaaagc tcattaaatt
    19321 tccctcatta ttaatattaa tcaacgttca aaggtggtac ttctcgcccc tgacgctggt
    19381 aaaaatcagt tacgaagctc tctaatttac cctcttcaat agccttgcgt aaacccgcca
    19441 tcaaacgctg gtagtaacga aggttatgaa tggtgttgag tcgcgcgcct aatatttcgt
    19501 tgcaacggtc aagatgatgc aagtaagcgc gtgaataatt gcgacaggtg tagcaatcac
    19561 actcaggatc aagtgggcca gtatcgctct tatacttcgc attgcggatt ttcaccacgc
    19621 catcggtcac gaacaaatga ccatttcggg cgttgcgggt tggcattacg cagtcaaaca
    19681 tatcgatacc acgacgtacg ccttcaacca ggtcttctgg tttaccaacg cccatcaggt
    19741 aacgcggttt gtctgccgga atttgcgggc atacatgctc cagaatgcgg tgcatatctg
    19801 ctttcggctc acccacagcc agaccgccga cagcgtagcc atcaaaaccg atatctacca
    19861 gacctttaac agaaatatca cgtaaatctt cgtaaacgct gccctgaatg ataccaaaca
    19921 gcgcattttt gtttccgaga ctgtcaaaac gctcacggct acgcttcgcc caacgcagag
    19981 acatctccat ggagcgtttt gcgtaatccc agtcagcagg atacggcgta cactcatcaa
    20041 agatcatgac gatatccgaa ccaagatcgt actgaatctc cattgatttt tcaggatcga
    20101 ggaaaatcgg atcgccgttg atcgggttac ggaagtgcac gccctgttcg gtgattttac
    20161 gaatatcgcc aaggctgaag acctggaagc cgccggagtc ggtgaggatc ggccccttcc
    20221 actgcataaa atcgtgcaga tcgccgtgca gtttcatgat ttcctggccc gggcgcagcc
    20281 acaggtggaa ggtgttgccg aggataattt gcgcgccagt ggcttcaact tcttccggcg
    20341 tcatcccttt tacggtgccg taggtgccaa caggcataaa acaaggcgtt tccactacgc
    20401 cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc ggtggtgtcc agttcaaatt
    20461 tcattttttc tccactacgt cagaaaaaca gtccaacgtt ttaaaccagc gccacggaat
    20521 tactccccga cgcgctcatt aattgcctgc ggattgtacg tgataaacat cgcatcaccg
    20581 taactaaaaa agcgatattt ctcttctacc gctgctttat aggcgttcat ggtgtgttga
    20641 taaccggcaa aggccgaaac cagcataatc agcgtcgatt ccggcaggtg gaagtttgtc
    20701 accagcgcat cgaccacttt gtactgaaag cccggataga taaagatttg ggtatcgtca
    20761 aagaacggtt cgataagatc gtttttcgcc gcctgagccg cgctttccag cgaacgtact
    20821 gaggtggtgc caaccgcaat cacccggtta ccgcgcgctt tcgccgccag taccgcgtct
    20881 accacatcct gcggcacttc agcgtattcc gagtgcatga tgtgatcttc aatggtgtcg
    20941 acgcgcaccg gctggaaggt gcccgcacca acgtgcaacg tcacaaacgc catctccacg
    21001 cctttggcgc gtaatttttc cagcaaaggt tcgtcaaaat gcagacctgc ggtcggggct
    21061 gcaaccgcgc ccggtttttc gctataaacg gtttgataaa gttcgcggtc agcgtcttcg
    21121 tccggacggt cgatatacgg cggcagcggc atatggccga tgctgttgag aatatccagc
    21181 accgagcgtt catcattaaa ttcgacttca aacagtgcgc cgtggcgcgc ggtcattgtt
    21241 gcgttaatac tttcgtcatc gcccagcagc agttctgcgc caggttttgg cgctttcgag
    21301 gcgcgaatat gcgcaagaat gcgtttgtcg tcgagcatcc gttcaaccag cacttcaatc
    21361 ttgccgccgc tggctttacg cccaaacagg cgcgccggga tcacgcgggt attattaaaa
    21421 accagaagat cgccggggtt gagcttatca agtaaatcgg tgaaagtacc gtgcgtcagc
    21481 gcgcccgtcg gcccgtccag cgacagtaaa cgacagctac tgcgttcagg catgggatag
    21541 tgggcaatca gggattcggg caattcaaag gagaaatcgg taacgcgcat gacactgact
    21601 cagactaaaa taagaggcgg gtagtctagt gccggggcgc tctccctgca acctttaccc
    21661 ccttttggat agaatataat cgatgaattt tttagctcac ctgcatttag cccatctcgc
    21721 ggaaagctcg ctttccggca atttactggc tgatttcgta cgcggaaatc ctgaagaaag
    21781 ttttccgccc gacgtcgtgg ctggcattca tatgcatcga cgtatcgacg tattaaccga
    21841 caatctgccg gaagtccgcg aagcacggga atggtttcgt agtgaaactc gccgcgttgc
    21901 gcctattacg ctggatgtca tgtgggatca ctttctttcc cgccactggt cgcagctgtc
    21961 gccggacttt ccgctacagg aatttgtctg ttatgcccgc gagcaagtga tgacgatttt
    22021 gccggactca ccgccacgtt ttatcaatct gaacaattac ttgtggtcag agcagtggct
    22081 ggtgcgctat cgcgatatgg atttcatcca gaacgtgtta aacggcatgg caagccgccg
    22141 cccacgtctg gatgccctgc gtgactcctg gtacgattta gacgctcatt atgacgccct
    22201 cgaaacccgc ttctggcagt tttatccgcg gatgatggcg caggcgtcac acaaggcgtt
    22261 ataaaacgtt agttcatcca taccgtagcc gaaatggcag gcaaagccag aatcccgtca
    22321 gtcagttgcc catgcctttc tttgcattgc cattgcacgg cattgagcaa cggtgacgcg
    22381 ggtagcacga cttcacaagc ttcgccacgg ttgattgcca ccagtacacg ctgctgattc
    22441 agcacgcgga caaataccac cacgttatct tccgcataca gcacctgaca gccgccatga
    22501 cgtagcgcct gacttttctt acgcagcgca atcattcgct ggtacagcgc gaataacgcc
    22561 gtatcctgct tttccacctg ccaggggaac ggtttacggc aaaacggatc gtttttgcca
    22621 tccagtccta cttcatcacc gtaataaatg cacggtacac caggccaggt gaacagccag
    22681 accaccgcca gcggcaggcg cgcaatatcc cgaccgagca gcgttttaaa tcgcgcagta
    22741 tcgtggctgt cgagctgatt aaacatacgt aattgttgtt gatgagaaag ccctgcgcgg
    22801 taattatcca tccaggccat acaggtttgg gcatcaattt gctgcggatc gtaagagata
    22861 tcggtattgg caagaaatcc ccacaacggg aatgtgaagc cacgatagtt catggcggca
    22921 tcttccacat cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc gacaatatac
    22981 gcttccggct gggtttcttt cgccgcttcg gtgctcccag caacgtgctg catattattg
    23041 cgcgccccac ccgcctcccc tagcatatgc accacatcca gccgccagcc gtccatactc
    23101 cacggcgctt tcagccaatg gcggacaata ctgtcttccc cgcgataaat ttcattcacc
    23161 agactttccg actgataatc cagcttcggc aagctggcat agccaagcca gtcgagcgcc
    23221 gtgccatcat cactaaacga gtaccagtcg cgccagggcg attcagggtt gtgacaagct
    23281 ccccccgtgc cacgattgtg cctatcaaac caggcatggg aatcgccact gtggttaaac
    23341 acgccatcca gccccagccg cattcccagc tgctgcgtat tgtggcgtaa gcgcagcaac
    23401 gccccatcac cgccaaactg cggatcgaca tggcgataat cctcggtatc gtatttatgt
    23461 acgctgggag ctttaaacac cggattgaga tacagcgccg tcacgccaag ctttttcaga
    23521 tacggcagtt tttcgcttat cccgtccaga tcgccgccat agaacgttga tccgcccgcc
    23581 tgcgccgtga ccggttcatc ccagtcacgc aagatgatct cttgtccggc tgcatgatgg
    23641 taatagacat gatcctgttc agcttcacga ggaagactac gcgcaaaacg atcagggaag
    23701 atctgataaa aaatctgatc cgcagcccat tgtgggccga tatccggtac atcgacggca
    23761 aactgctcca gtcgtgccgg cggcattcgg ctgaagccct gcggtgtaaa ccaacgctgg
    23821 cgatcgtgcc acagcagttt gaaactgtaa cgccgccggg gttgtccgct ggagagatca
    23881 atcgccgcac gccatgcggt gacgccaggc tgctgctgac tgcgatgctt atgcatcgat
    23941 actgacattt cttcgttatc gtgttctgta cgcagcataa tgcgctgcgg tgggtcttcg
    24001 cccgtcagcc acagtgtaat gagcagttga tctttgcttt gtttaacaaa tgggggcacc
    24061 ggcaggtgcc atgcatttaa catcataatt cccctgtgat gaaaatctga tcaccttgcc
    24121 atagcctaat gcaggatcac tcatcatctg aaggatattt gggggaggag gacgggggcg
    24181 gataactttc ggaatagccg gatcaggatt tcctcctgaa ccggcttaca gaacgcaagg
    24241 attactggtt ttcagccagc tgacgatcgt ggcgacgctt aaacatccag ccaatcaaca
    24301 gcacaacaat ccacgcgaaa ccgacataca gcgagatacg cgtatccggg tgataaccaa
    24361 tcaacccgat aataaagagc aggaaaatca gcccgccgat ggtcgttgct accccacccg
    24421 gcactttaaa tttcagcgcc ttaacttctt ctggcggcaa acagcgacgg aaggcaattt
    24481 gcgacagcag gatcataatc cacacccaca ccgtggcgaa ggttgccagc gaagcgatca
    24541 ccaggaagac gttttccggc atgatgtagt tcagatacac cgcaaacagc agcgcggtag
    24601 tcatcaccag caccgtaacc cacggaatac cgcgacgcga cgttttgctg aaaattttcg
    24661 gcgcgctgcc ctgctctgcc ataccgtgga gcatacggcc tacgccaaag acgtcactgt
    24721 taattgccga tagcgaagcg gtcagcacaa caaagttaag aatgctagcg gcaaaggtaa
    24781 tgcccatatg ctggaacgtc agcacgaacg ggctaccggc agtgccaacc tgattccacg
    24841 ggtagataga cataatgacg aacagcgtac cgacgtagaa caccagaata cgcatcggca
    24901 cggagttaat cgcacgcggt atcgatttct caggatcttt cgcttcaccg gcggtaatcc
    24961 cgataatttc gatcccaccg taagcaaaca tcaccatttg caacgacatc accatcccaa
    25021 gccagccgtt actgaagaag ccgccgttgc tccacagatt atgaataccg gtcggttgcc
    25081 cgccgttgcc aatcccccag atgatgatgc cgaaaccagc gacaatcatg atgatgatgg
    25141 tggcgacttt aaagaacgag aaccagaatt ccagctcccc gaagaccttc acgctcatca
    25201 ggtttacggc gcaaatgatc agcaccacgc tcagtaccca aatccagtgc ggcaccgtcg
    25261 ggaaccagac acccatatag ataccaaaag cggtcacatc ggcgatggcg acaataagga
    25321 tttcaaagca gtaggtccag ccggtaatgt aacctgccag cgggccgagg ttttcctgcg
    25381 cataacgcga gaaagagctg gcggccgggt tatgtaccga catttccccc agcgcacgca
    25441 taatgatata cgccgcgata ccaccgataa tataggccaa caacacgctc ggaccggcca
    25501 ttttgatggc gtctgccgaa ccgtaaaaca gcccggtacc aattgctgaa cccagtgcca
    25561 taaagcgtat gtggcgggta cttagcccac gctttagctt gttcttactt tccatcaatt
    25621 cctaacccat caacacaata aaaaaccacg gggcatcgca gccccgtggt taaaacaaat
    25681 gttcagtgat ttagtgagcg ctggaggtca cctgacgacc tgccgcgcga tcccagataa
    25741 tggccagaac caccatcacc actgttggca ttaaccacgc cagaccttgt tcggccagcg
    25801 gtaaacgctg cgcccaggac ggtaagatat cgctgaatgc agatgctttg atcccgtcga
    25861 gaataccaaa aagcaggctg ataaacatcg gcggagcaat cacgcgggac gaattatgcc
    25921 accatgagcg tgtaaaactt aatacaacca gtgcgataca cggcggataa atagcggtca
    25981 gcaccggtac ggagatctgg atcagctggc ttaagccgag gttagaaacc accatcgaga
    26041 agccgccgag gataaacacc agcgtacgat aagagagcgg tacgtactgg gcaaagaatt
    26101 ctgcacaagc acaggtcagg ccaactgccg ttaccaagca ggcgatgaag attaacgccg
    26161 ccagcaggaa gctaccgccg ccgccaaagg tgtgctgaac gtaagcatgc agaatagcag
    26221 cgccgtttgc agactgatcg accagcgacg cgctgtctga acccagacgg aacagcgcca
    26281 ggtacagcag agtcagacca acacccgcca tcaggccagc ccagacggta taacgggtca
    26341 gcagacgcgc ttcggtaacg ccacgagaac gcgccgcgtt aacaataacg ataccaaaca
    26401 ccattgcgcc cagcgtatcc atggtcagat agccgttaac gaagccgtta gaaaacgcag
    26461 cgttttgata agcctcagtc gccgtgctga tagaacccgc cggccagaca atcgcggcaa
    26521 cagacaggat gaccagcgcg ataattttca gcggcgcaag gaagttgccc acggtatcca
    26581 gcagcttgcc cggatagagc gaaaccagaa taacgatagc gaaatagacc aggctgtaga
    26641 taaacagcgg cagcgcggaa tcaccagtca gcggcgcaat ccccacttca aaggaaacgg
    26701 tagctgtacg cggcgtagcg aaaagcggcc ccaccgccag gtaacaaacc gttgccagca
    26761 gcacgccagc gactttaccg attggcgtgc tgaggctgtc aacaccgccg ccaacttttg
    26821 ccagcgccac taccgttaat accggcaggc caacggcagt aatgaggaag ccgaatgccg
    26881 cagtccagac gtgttcgcca gcctgcaagc cgaccattgg agggaaaata atgttacctg
    26941 cgccgacgaa caacgcaaat gtcataaagc ccagagcgat gatatcgcgc gatcttaatt
    27001 gatgggtcat aaatcatact gcctgtggat gtggtgttgt aacgttaaat ttttgtgcca
    27061 atccctgggg atgatacagc ggtatattta aacaatttta gcgggaaata ctcccaacca
    27121 ggcgcggcga agaatagttt ttcgatttac cgcgttaatg cagtcagtat gacagcacct
    27181 gaagcgcaat ttaaacgctt ataacgtttt aaagcaagga gggatctaaa aaccagaggt
    27241 atatagcgat aagaaatcac caaccagtat aaaacactat ttccgtgaaa aagacatggc
    27301 taatcatgcg aacaaaaaat caccaaccgt tgtaaaatct aacgcccgta ttttgactgc
    27361 aaagaaaata agccagcatc gactggctta tggcaataaa agatgacaaa ggcggattaa
    27421 tcgctgtttt tggcaattaa acgttccggg ataacaaaac tgaaacgtgt tccttttcct
    27481 actgtactct caatattcag gcgactttcg tgatgattca cagcatgttt cacgatcgct
    27541 aaccctaatc cgctaccgcc ggtttgccgg gaacgcgctt tatcaacgcg ataaaaacgc
    27601 tcggtcaggc gcggaatatg ctccggtgca atgcccggtc cgttatcttc aacgctaaat
    27661 tcggcaccgt gcggcactcg ctgccagcgt acggtgatat gcgtgccttc tggagtatga
    27721 ttcacggcgt tatagaccag gttcgaaatc gcactgcgta gctgatcttc gttgccagac
    27781 actttgaggc cgttatctat ctcaaaggta aatgtctgtt ttttctgact cagagtctga
    27841 gcctcgcgct caacaacgcg cagcatcatc ggcacatcaa ccttttcatt gagcaattgc
    27901 gtcggcgcgg cttcaatttt cgacagcgtt agcaactgct tcaccagccc ttccatccgc
    27961 tgggtctgct cgcgcatggt gtgcaacgct ttttcgcgta ccgcgccttc cagcggctgc
    28021 tcatccatca tctccaggta accctgtaac acggtcaatg gcgtacgtaa ctcatggctc
    28081 acgttggcaa aaaagttacg ccgcgcccct tccagttgat gcatttgcgt gacatcacgc
    28141 gccaccatca gcaactgttt gtgggtataa ggcatgacgc gaatttccag atgccgcccg
    28201 gtgttgagca ccagattgag cgggcgagaa aaatcacgcg ttttcagata ttgcgtaaac
    28261 tccgggtaac gcagtaggtt aaggatgttc tgcccgttat cttccggcca gcgcaaacca
    28321 agaatttgtt gcgccagacc gttacaccag aaaataccgc tctcttccgt ggtcagcacc
    28381 accgcgtcgg gcagcgactc cgcgccgcta cgaaagcgtt taatcagatt gcccagttca
    28441 cggcggcgtt ttttatttcg cagctgcatc tggtgtaagc cgtatagtag cggttcccag
    28501 ctaccacgcc ccggtggcgg ggtcatactg cgatccaccc acagccacca tgaaaggcgc
    28561 aataaattcc agaaatgcca gataagcagt cctgttaccg atgccagcaa aaaccagggc
    28621 aggtaaccaa aaaatgcacc caggatgaaa gccgggaggc agcaaagtag cagctccagc
    28681 accagccttt tccacgacag ccgttccagc acgtaagata ctcccgttaa gaaatcataa
    28741 gccctgctct gcgtccgatg agcaaggcgt taaaagcggg ttgaaaaacg atatcctgta
    28801 ccgcgcacgg tctgcaccat gcggtcatgc ccgccgggct ccagtgcttt acgcaggcga
    28861 cgaatgtgga catcgaccgt gcggtcttcc acatacacgt tagttcccca gacgtggttt
    28921 aacagctgct cgcggctgta cacgcgctca ggatgcgtca taaaaaagtg cagcagttta
    28981 aattctgtcg gccccatctc cagcggctct tcgcccgcca tcactcggtg agatgtcggg
    29041 tcgagactta atccctgcat ctcaatcacc tcttccaccg ccattggcga aatacggcgc
    29101 attaccgctt tgattcgcgc caccagctcc ttcggcgaaa acggcttggt gatatagtca
    29161 tccgcgccgg tttcaaggcc gcgcacgcga tcttcttctt cccctctggc ggtcaacatc
    29221 accactggaa tatcccgggt catcgactcg cgcttaaggt gtttgatgaa ctggataccg
    29281 gagccgccag gtaacatcca gtcaaggaga attaaatccg gccagggttc attcagttga
    29341 ttcacagcac agtcataatc ttccgcttcg accggctgaa agccattttg ttcgagcacg
    29401 aagcagacca tttcacgaat tggagcttca tcttctacga ccagaatacg tctcgccatg
    29461 atttgccctg ttgtaataaa taggttgcga tcattaatgc gacgtcatta tgcgtcagat
    29521 ttatgacaga tttatgaaaa gctcgtcgca catatcttca ggttattgat ttccgtggcg
    29581 cagaaaaaag caaatggcac atctgtttgg gtataatcgc gcccatgctt tttcgccagg
    29641 gaaccgttat gcgcatcctt cacacctcag actggcatct cggccagaac ttctacagta
    29701 aaagccgcga agctgaacat caggcttttc ttgactggct gctggagaca acacaaaccc
    29761 atcaggtgga tgcgattatt gttgccggtg atgttttcga taccggctcg ccgcccagtt
    29821 acgcccgcac gttatacaac cgttttgttg tcaatttaca gcaaactggc tgtcatctgg
    29881 tggtactggc aggaaaccat gactcggtcg ccacgctgaa tgaatcgcgc gatatcatgg
    29941 cgttcctcaa tactaccgtg gtcgccagcg ccggacatgc gccgcaaatc ttgcctcgtc
    30001 gcgacgggac gccaggcgca gtgctgtgcc ccattccgtt tttacgtccg cgtgacatta
    30061 ttaccagcca ggcggggctt aacggtattg aaaaacagca gcatttactg gcagcgatta
    30121 ccgattatta ccaacaacac tatgccgatg cctgcaaact gcgcggcgat cagcctctgc
    30181 ccatcatcgc cacgggacat ttaacgaccg tgggggccag taaaagtgac gctgtgcgtg
    30241 acatttatat tggcacgctg gacgcgtttc cggcacaaaa ctttccacca gccgactaca
    30301 tcgcgctcgg gcatattcac cgcgcacaga ttattggcgg catggaacat gttcgctatt
    30361 gcggctcccc cattccactg agttttgatg aatgcggtaa gagtaaatat gtccatctgg
    30421 tgacattttc aaacggcaaa ttagagagcg tggaaaacct gaacgtaccg gtaacgcaac
    30481 ccatggcagt gctgaaaggc gatctggcgt cgattaccgc acagctggaa cagtggcgcg
    30541 atgtatcgca ggagccacca gtctggctgg atatcgaaat cactactgat gagtatctgc
    30601 atgatattca gcgcaaaatc caggcattaa ccgaatcatt gcctgtcgaa gtattgctgg
    30661 tacgtcggag tcgtgaacag cgcgagcgtg tgttagccag ccaacagcgt gaaaccctca
    30721 gcgaactcag cgtcgaagag gtgttcaatc gccgtctggc actggaagaa ctggatgaat
    30781 cgcagcagca acgtctgcag catcttttca ccacgacgtt gcataccctc gccggagaac
    30841 acgaagcatg aaaattctca gcctgcgcct gaaaaacctg aactcattaa aaggcgaatg
    30901 gaagattgat ttcacccgcg agccgttcgc cagcaacggg ctgtttgcta ttaccggccc
    30961 aacaggtgcg gggaaaacca ccctgctgga cgccatttgt ctggcgctgt atcacgaaac
    31021 tccgcgtctc tctaacgttt cacaatcgca aaatgatctc atgacccgcg ataccgccga
    31081 atgtctggcg gaggtggagt ttgaagtgaa aggtgaagcg taccgtgcat tctggagcca
    31141 gaatcgggcg cgtaaccaac ccgacggtaa tttgcaggtg ccacgcgtag agctggcgcg
    31201 ctgcgccgac ggcaaaattc tcgccgacaa agtgaaagat aagctggaac tgacagcgac
    31261 gttaaccggg ctggattacg ggcgcttcac tcgttcgatg ctgctttcgc aggggcaatt
    31321 tgctgccttc ctgaatgcca aacccaaaga acgcgcggaa ttgctcgagg agttaaccgg
    31381 cactgaaatc tacgggcaaa tctcggcgat ggtttttgag cagcacaaat cggcccgcac
    31441 agagctggag aagctgcaag cgcaggccag cggcgtcacg ttgctcacgc cggaacaagt
    31501 gcaatcgctg acagcgagtt tgcaggtact tactgacgaa gaaaaacagt taattaccgc
    31561 gcagcagcaa gaacaacaat cgctaaactg gttaacgcgt caggacgaat tgcagcaaga
    31621 agccagccgc cgtcagcagg ccttgcaaca ggcgttagcc gaagaagaaa aagcgcaacc
    31681 tcaactggcg gcgcttagtc tggcacaacc ggcacgaaat cttcgtccac actgggaacg
    31741 catcgcagaa cacagcgcgg cgctggcgca tattcgccag cagattgaag aagtaaatac
    31801 tcgcttacag agcacaatgg cgcttcgcgc gagcattcgc caccacgcgg cgaagcagtc
    31861 agcagaatta cagcagcagc aacaaagcct gaatacctgg ttacaggaac acgaccgctt
    31921 ccgtcagtgg aacaacgaac tggcgggttg gcgtgcgcag ttctcccaac aaaccagcga
    31981 tcgcgagcat ctgcggcaat ggcagcaaca gttaacccat gctgagcaaa aacttaatgc
    32041 gcttgcggcg atcacgttga cgttaaccgc cgatgaagtt gctagcgccc tggcgcaaca
    32101 ttccgagcaa cgcccactgc gtcagcgcct ggtcgcgctg catgggcaga ttgttcccca
    32161 acaaaaacgt ctggcgcagt taatggtcac tatccagaat gtcactctgg aacagacgca
    32221 acgtaatgtc gcacttaacg aaatgcgcca gcgttataaa gaaaagacgc agcaacttgc
    32281 cgatgtgaaa accatttgcg agcaggaagc gcgcatcaaa acgctggaag ctcaacgtgc
    32341 acagttacag gcgggtcaac cttgtccact ttgtggttcc accagccacc cggcggtcga
    32401 ggcgtatcag gcgctggagc ctggtgttaa tcagtctcga ttactggcgc tggaaaacga
    32461 agttaaaaag ctcggcgaag aaggtgcggc gctgcgcggg caactggatg cattaacgaa
    32521 gcagcttcag cgcgatgaaa acgaagcgca aagcctccga caagatgagc aagcacttac
    32581 tcaacaatgg caagccgtca cgaccagcct caatatcacc ttgcagccac aggacgatat
    32641 tcaaccgtgg ctggatgcac aagatgagca cgaacgccag ctgcggttac tcagccaacg
    32701 acatgaatta caagggcaga ttgccgcgca taatcagcaa attatccagt atcaacagca
    32761 aattgaacaa cgccagcagc agctcttaac ggcattgacg ggttatgcac tgacattgcc
    32821 acaggaagat gaagaagaga gctggttggc gacacgtcag caagaagcgc agagctggca
    32881 gcaacgccag aacgaattaa ccgcgctgca aaaccgtatt cagcagctga cgccgattct
    32941 ggaaacgttg ccgcaaagtg atgatctccc gcacagcgaa gaaactgtgg cgctggataa
    33001 ctggcggcag gttcatgaac aatgtctcgc attacacagc cagcagcaga cgttacagca
    33061 acaggatgtt ctggcggcgc aaagtctgca aaaagcccag gcgcagtttg acaccgcgct
    33121 acaggccagc gtctttgacg atcagcaggc gttccttgcg gcgctaatgg atgaacaaac
    33181 actaacgcag ctggaacagc tcaagcagaa tctggaaaac cagcgccgtc aggcgcaaac
    33241 gctggtcaca cagacagcag aaacgctggc acagcatcaa caacaccgac ctgacgggtt
    33301 ggctctcact gtgacggtgg agcagattca gcaagagtta gcgcaaactc accaaaagtt
    33361 gcgtgaaaac accacgagtc aaggcgagat tcgccagcag ctgaagcagg atgcagataa
    33421 ccgtcagcaa caacaaacct tactgcagca aattgctcaa atgacgcagc aggttgagga
    33481 ctggggatat ctgaattcgc taataggttc caaagagggc gataaattcc gcaagtttgc
    33541 ccaggggctg acgctggata atttagtcca tctcgctaat cagcaactta cccggctgca
    33601 cgggcgctat ctgttacagc gcaaagccag cgaggcgctg gaagtcgagg ttgttgatac
    33661 ctggcaggca gatgcagtac gcgatacccg taccctttcc ggcggcgaaa gtttcctcgt
    33721 tagtctggcg ctggcgctgg cgctttcgga tctggtcagc cataaaacac gtattgactc
    33781 gctgttcctt gatgaaggtt ttggcacgct ggatagcgaa acgctggata ccgcccttga
    33841 tgcgctggat gccctgaacg ccagtggcaa aaccatcggt gtgattagcc acgttgaagc
    33901 gatgaaagag cgtattcctg tgcagatcaa agtgaagaag atcaacggcc tgggctacag
    33961 caaactggaa agtacgtttg cagtgaaata actattcagc aggataatga atacagaggg
    34021 gcgaattatc tcttggcctt gctggtcgtt atcctgcaag ctatcacttt attggctacg
    34081 gtgattggta gccgttctgg tggttgtgat ggtggtatga aaaaagtcat tttatctttg
    34141 gctctgggca cgtttggttt ggggatggcc gaatttggca ttatgggcgt gctcacggag
    34201 ctggcgcata acgtaggaat ttcgattcct gctgccgggc atatgatctc gtattatgca
    34261 ctgggggtgg tggtcggtgc gccaatcatc gcactctttt ccagccgcta ctcactcaaa
    34321 catatcttgt tgtttctggt ggcgttgtgc gtcattggca acgccatgtt cacgctctct
    34381 tcgtcttacc tgatgcttgc cattggtcgc ctggtttccg gctttccgca tggcgcattt
    34441 tttggcgtcg gcgcgatcgt gttatcaaaa attatcaaac ccggaaaagt caccgccgcc
    34501 gtggcgggga tggtttccgg gatgacagtc gccaatttgc tgggcattcc gctgggaacg
    34561 tatttaagtc aggaatttag ctggcgttac acctttttat tgatcgctgt ttttaatatt
    34621 gcggtgatgg catcggtcta tttttgggtg ccagatattc gcgacgaggc gaaaggaaat
    34681 ctgcgcgaac aatttcactt tttgcgcagc ccggccccgt ggttaatttt cgccgccacg
    34741 atgtttggca acgcaggtgt ctttgcctgg ttcagctacg taaagccata catgatgttt
    34801 atttccgttt tttcggaaac ggcgatgacc tttattatga tgttagttgg gctagggatg
    34861 gtgctgggaa atatgctaag tggcaggatt tcaggacgtt attcaccact gcgcattgca
    34921 gcagtgactg actttatcat tgtactggca ctgctgatgc tctttttctg cggcggcatg
    34981 aaaacaacgt cgcttatttt tgcttttatt tgttgcgcgg gattatttgc cctttcagca
    35041 cctctgcaaa tattgttact gcaaaacgcc aaaggcggag agttattagg tgccgcaggt
    35101 gggcaaatag cgtttaacct cggtagcgcc gtcggcgcat attgcggagg tatgatgctg
    35161 acgctggggc tggcatataa ttacgtggcg ctgcctgccg ccctgctttc gtttgctgcg
    35221 atgtcatcgt tgctgctgta tggtcgctat aagcgccagc aagcggcgga tagtccggtg
    35281 ctggcgaaac cactggggta gattactgtc acggtgcgcg ttgcttattg ccggatgcgg
    35341 cgtgaacgcc ttatccgccc tacgcggttc tggcacattt tgtaggcctg ataagacgcg
    35401 gcaagcgtcg catcaggcat gatgcgccaa ttgcctacgt tttttactct tgtggccata
    35461 accacgcagc gccgcgtacg ccgctggaat caccgtgctt cgccttacgc accggcgttt
    35521 cacattcgcc gccgaagaca aattgtttaa tcaactgccc aaccgtttga tataaacggt
    35581 ctacattgct catcccgccc cccaggacaa tcacatccgg atcgagaata ttcacgacat
    35641 gtgccagcga ttttgccagc cgcagctcgt agcgacgcaa tgccagttcc gctaccggat
    35701 cgctttcttc aaccaggcgg ataatttcac tgcctttcag cgcatgtccg ctcaaacgac
    35761 gataatccgt cgcgaatccc gtgcccgaaa taaaggtttc aatacaacct tgtttaccgc
    35821 aataacaagg gacttcctcg cgataacgca gctcgtcttc atccatccac ggtagcggat
    35881 tgtgtcccca ctcgcctgcc gtgccattgc cgccgatatg cgcccgccca tttaatgcca
    35941 cgcccgcgcc gcatcccgtg ccgataatca cggcaaatac cgtctgcgct cccgctgccg
    36001 cgccatctac tgcttctgaa accgccagac agttggcatc atttgccagc cgcacttccc
    36061 gctgcaacct cgcgcttaag tctttatcga atggctgacc gttgagccag gttgaattgg
    36121 cattcttcac cacaccggtg tagggcgaaa tcgaacctgg aatgcccata cctaccgttc
    36181 cgcgctgccc tgtcgcctgc tccgccatat caaccaacgt ggcgatcgtt tcaatagtct
    36241 gccggtaatc atcacgcggc gtgggcagac gatggcggta caactgctcc cctgcatcgc
    36301 ccagtgcaat cacttcagtt ttggtgccgc ctaaatcgat acctatacgc actgtactct
    36361 ccttattttt ttcaatatca atagcgtaga gacggacaac cggattggca atgcaaggcc
    36421 gccgacaatt cgttatcatg cccgctaaat ttaacgacaa ggccgtggaa attatcatgc
    36481 tgtggttcaa aaatttaatg gtttaccgtc ttagccgcga gatttcgctg cgtgcagaag
    36541 agatggaaaa acagctagcc tcgatggcat ttaccccatg cggcagccag gacatggcga
    36601 agatgggctg ggttcctccg atgggatcgc acagcgatgc gttaacgcac gttgccaatg
    36661 gtcaaattgt tatctgcgcg cgcaaagaag aaaaaatcct cccgtctccg gtgattaaac
    36721 aggcgctgga agcgaaaatc gccaaactgg aagcggaaca ggcgcgtaag ctgaagaaaa
    36781 ccgaaaaaga ttcgctgaaa gacgaagtgc tgcactctct gctgccgcgt gctttcagcc
    36841 gttttagcca gacaatgatg tggatcgaca cggttaacgg tttgattatg gtggactgcg
    36901 ccagtgccaa aaaagcggaa gatacgctgg cattactgcg taaaagcctg gggtcgttac
    36961 cggttgtacc attgagcatg gaaaacccga ttgaactgac gctgaccgaa tgggttcgct
    37021 ccggtagtgc ggcacagggc ttccagctgc ttgatgaggc cgagctgaaa tcgttgctgg
    37081 aagatggcgg cgtgatccgc gcgaagaaac aagatctgac cagcgaagag atcaccaatc
    37141 acattgaagc cggaaaagtg gtgactaaac tggcgctcga ctggcagcag cgcattcagt
    37201 ttgtgatgtg cgacgatggt tcgctcaagc gtcttaagtt ctgcgacgag ctgcgcgatc
    37261 aaaacgaaga tatcgaccgt gaagatttcg ccctgcgttt tgatgccgat ttcatcctga
    37321 tgactggtga actggcagcg ttaattcaaa acctgattga aggattaggt ggcgaagcac
    37381 aacgttaatt gctgattttc ctttaatgcc ggatgcgacg cctgccgcgt cttatccggc
    37441 gtacgaagcc acaccaggca tataattatt cgctacggcg agcaataatt tttagcgcag
    37501 caatattatg cgttttacgc cgtaactgat ctaatcgcct catccctatt cgcctgtcag
    37561 cagcacgcag aatattcact aacccccatt tatcgctggt aaattgtttt gttaacagct
    37621 ccgcttttgg ggtatcaata tattcacgca ataaatcaga aattgaacaa atatccgtca
    37681 ggtaaggata ccagcaagtt tcaccgtgaa aattacgcac tccagcgtta cctgcaataa
    37741 agtctttcgg gtaatcccag atcacgtttt tatccaacgt gatccaataa cgcggtaaat
    37801 cggtacttcc accattttgg ctgcgcatga gataacgggt acagtgaatt tgcaggttga
    37861 tcgttggcgt cagtaagtca tagatctccc gctgtaactt gctccatgga tgttgtgtca
    37921 ttgtttttcc tcaagccggt gacgcgcttc actgcgaatt ttccgcgttg ccccggtttt
    37981 cactaaatca cgtaactgcg aaagatcggg ttgttcgaca aataagagta atgatagatc
    38041 ctcttttaac cacgccgttt tcacatcggc agccagctgt ggattattaa tagccagcga
    38101 ccgatcctga gactcagtca atgtcgttaa caacgacgcc ggggtatgtg cgttgcgtaa
    38161 tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat gcacgccacc attcatcatc
    38221 tgatttatct tttatcgtag ccagttccgc aaaaaatacc tggagatctt cactgatatt
    38281 tatatcaacc ttgatgaatt atgaatatat cggaaattga aaaaatcgca gcaaagga
//
LOCUS       DAJTVJ010000029        37082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000029 DAJTVJ010000000
VERSION     DAJTVJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.029"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..389)
                     /locus_tag="OO448_001409"
     CDS             complement(<1..389)
                     /locus_tag="OO448_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR6336660.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPL"
     gene            complement(567..1478)
                     /gene="gsiD"
                     /locus_tag="OO448_001410"
     CDS             complement(567..1478)
                     /gene="gsiD"
                     /locus_tag="OO448_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6336661.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1481..2401)
                     /gene="gsiC"
                     /locus_tag="OO448_001411"
     CDS             complement(1481..2401)
                     /gene="gsiC"
                     /locus_tag="OO448_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6336662.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2419..3957)
                     /gene="gsiB"
                     /locus_tag="OO448_001412"
     CDS             complement(2419..3957)
                     /gene="gsiB"
                     /locus_tag="OO448_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6336663.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3977..5848)
                     /gene="gsiA"
                     /locus_tag="OO448_001413"
     CDS             complement(3977..5848)
                     /gene="gsiA"
                     /locus_tag="OO448_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6336664.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5835..6800)
                     /gene="iaaA"
                     /locus_tag="OO448_001414"
     CDS             complement(5835..6800)
                     /gene="iaaA"
                     /locus_tag="OO448_001414"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6336665.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7004..8239
                     /gene="moeA"
                     /locus_tag="OO448_001415"
     CDS             7004..8239
                     /gene="moeA"
                     /locus_tag="OO448_001415"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6336666.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8239..8988
                     /gene="moeB"
                     /locus_tag="OO448_001416"
     CDS             8239..8988
                     /gene="moeB"
                     /locus_tag="OO448_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6336667.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9167..9829)
                     /gene="fsa"
                     /locus_tag="OO448_001417"
     CDS             complement(9167..9829)
                     /gene="fsa"
                     /locus_tag="OO448_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6336668.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9960..10859
                     /locus_tag="OO448_001418"
     CDS             9960..10859
                     /locus_tag="OO448_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6336669.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10865..13297
                     /locus_tag="OO448_001419"
     CDS             10865..13297
                     /locus_tag="OO448_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6336670.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13443..14258
                     /gene="ybiV"
                     /locus_tag="OO448_001420"
     CDS             13443..14258
                     /gene="ybiV"
                     /locus_tag="OO448_001420"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6336671.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14410..15675
                     /locus_tag="OO448_001421"
     CDS             14410..15675
                     /locus_tag="OO448_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6336672.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15642..16802)
                     /locus_tag="OO448_001422"
     CDS             complement(15642..16802)
                     /locus_tag="OO448_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6336673.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17040..18341
                     /locus_tag="OO448_001423"
     CDS             17040..18341
                     /locus_tag="OO448_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6336674.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18356..20719
                     /locus_tag="OO448_001424"
     CDS             18356..20719
                     /locus_tag="OO448_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCR6336675.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20899..22494)
                     /locus_tag="OO448_001425"
     CDS             complement(20899..22494)
                     /locus_tag="OO448_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6336676.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22713..23633
                     /gene="ldtB"
                     /locus_tag="OO448_001426"
     CDS             22713..23633
                     /gene="ldtB"
                     /locus_tag="OO448_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6336677.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23692..24810)
                     /locus_tag="OO448_001427"
     CDS             complement(23692..24810)
                     /locus_tag="OO448_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6336678.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24807..25274)
                     /gene="mntR"
                     /locus_tag="OO448_001428"
     CDS             complement(24807..25274)
                     /gene="mntR"
                     /locus_tag="OO448_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6336679.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25460..25588
                     /gene="mntS"
                     /locus_tag="OO448_001429"
     CDS             25460..25588
                     /gene="mntS"
                     /locus_tag="OO448_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6336680.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25860..27443
                     /gene="opgE"
                     /locus_tag="OO448_001430"
     CDS             25860..27443
                     /gene="opgE"
                     /locus_tag="OO448_001430"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6336681.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27492..28007)
                     /gene="ompX"
                     /locus_tag="OO448_001431"
     CDS             complement(27492..28007)
                     /gene="ompX"
                     /locus_tag="OO448_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6336682.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28060..28125)
                     /locus_tag="OO448_001432"
     CDS             complement(28060..28125)
                     /locus_tag="OO448_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336683.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28360..29247
                     /gene="rhtA"
                     /locus_tag="OO448_001433"
     CDS             28360..29247
                     /gene="rhtA"
                     /locus_tag="OO448_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6336684.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29546..30049
                     /gene="dps"
                     /locus_tag="OO448_001434"
                     /gene_synonym="pexB"
     CDS             29546..30049
                     /gene="dps"
                     /locus_tag="OO448_001434"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6336685.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30453..31199
                     /gene="glnH"
                     /locus_tag="OO448_001435"
     CDS             30453..31199
                     /gene="glnH"
                     /locus_tag="OO448_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6336686.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31338..31997
                     /gene="glnP"
                     /locus_tag="OO448_001436"
     CDS             31338..31997
                     /gene="glnP"
                     /locus_tag="OO448_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6336687.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            31994..32716
                     /gene="glnQ"
                     /locus_tag="OO448_001437"
     CDS             31994..32716
                     /gene="glnQ"
                     /locus_tag="OO448_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6336688.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            32833..35058
                     /gene="ybiO"
                     /locus_tag="OO448_001438"
     CDS             32833..35058
                     /gene="ybiO"
                     /locus_tag="OO448_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR6336689.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35055..35981)
                     /gene="rlmF"
                     /locus_tag="OO448_001439"
     CDS             complement(35055..35981)
                     /gene="rlmF"
                     /locus_tag="OO448_001439"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6336690.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36257..36517
                     /locus_tag="OO448_001440"
     CDS             36257..36517
                     /locus_tag="OO448_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6336691.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36782..>37082
                     /locus_tag="OO448_001441"
     CDS             36782..>37082
                     /locus_tag="OO448_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6336692.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         8983 a        10017 c         9300 g         8782 t
ORIGIN      
        1 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa
       61 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
      121 tccattttca gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag
      181 tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga
      241 ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta
      301 atgatgaaaa aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt
      361 atctttatct tttcgtataa actcagcatg ttaatacatt taacaaaata actatctgaa
      421 cgctgtgccg ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct
      481 acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta
      541 ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt
      601 aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa
      661 acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac
      721 tctggtgtcg gcggctgcgc accgaggccg agaaatgaca ggctggcggc agagataatc
      781 gaggtgccaa tgcgcatggt gaaaaacacc acgatagacg agacggtccc aggcaagata
      841 tgacgcaata aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt
      901 tgctgtttca acaccagcgt gttgccgcgc accagtcggg caaacgcggg gatggaaaaa
      961 atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc
     1021 gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc
     1081 caccagcctt cataatatcc agccaacaag cccagcaacg tcccgatcgc cgcaccgata
     1141 aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa
     1201 atgtcgcgcc ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga
     1261 ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc
     1321 accacaatca ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat
     1381 cgtcgccaga attcatgcca cggtgtacgc acctggtcag gtttgaccag tggcatggcg
     1441 tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg
     1501 ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga
     1561 aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttctacggag tcaacgagta
     1621 agcgtccaag tccgggccag ttgaaaactt tctccaccac gatagaacca ccgagcaaaa
     1681 agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttacgt agcccgtgtt
     1741 tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc
     1801 ttaaaacatc gacaaacgac gcgcgggtaa agcgcgccat cacggctgcc actgccgcgc
     1861 cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccacg gtaggcagcc
     1921 agcccagttc aacggagaat acctgaatta aaagcatccc cagcgcaaat gccggaaacg
     1981 agatccccga caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg
     2041 cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca
     2101 gccacagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca
     2161 tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca
     2221 gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcga
     2281 gcccggcaat caatcgcgcc ggatcgccgg gcagcatatg cacaaataaa aacaccagca
     2341 ccgagacgat aaacagcgtc ggaatcaacc ccagtaagcg tttgataacg taattaagca
     2401 ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cggtgtctgg
     2461 catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag
     2521 cgggatccac ggcgactctt gccagatgat atcctgcgcc gccttataca agcgggtctt
     2581 ttccgccgga tcattagttt tcagtgcctg agccaggaag tcgtccacct gtttattgct
     2641 gtaaaatgcg gtattaaaca gcgtcggtgg ccagttttgc gaggcgaaca gtggcgatag
     2701 cgcccagtcc gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct
     2761 ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac
     2821 ctgggcttta atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc
     2881 ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc
     2941 tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta
     3001 ggcgatactt ggcggtacca caccagtagc tggcgttgca tagcccgcaa aggcaacttt
     3061 caccagcgcc ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa
     3121 cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa
     3181 ctcgatattt ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa
     3241 ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca
     3301 ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt
     3361 cacaaaatcg gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa
     3421 accaatctcc ttgccatatt tttccagcgc tgccggtgaa atcatcgcgg tcgccggatg
     3481 ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg
     3541 atcgatcgcc tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc
     3601 cggatcgctg gcccggtcca gattcgcttt taccgccact gcgttgaaat cggtgccatc
     3661 ctggaattta atgccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt
     3721 ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg
     3781 gtaaaacgat ttcgctacgg cctgagataa cgtatcattc gcgtcatacg gatcgagcgt
     3841 ggtgaaattc gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat
     3901 caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt
     3961 attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt
     4021 aatgccccgg cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa
     4081 tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag
     4141 cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg
     4201 cgcgccgtgg accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa
     4261 tccgctctac cacggccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct
     4321 ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc gccgaaacgg
     4381 cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct
     4441 ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca
     4501 cgcgctccag caaccacgca acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta
     4561 cacgcagcgg ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag
     4621 ggtcctgaaa aataaactga atatcccggc gcaatgcctg aagtttgccg ggtgacaagg
     4681 tatcgattct ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca
     4741 acgcccgccc ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc
     4801 caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca
     4861 acaaaccgct gcgcaaaggg aaacgggtga caagattacg cactcgtaaa acaggttcgc
     4921 catccaccac cgttttctgc tcgatggggg gggcctgttt cgctggatgt tcaagcgata
     4981 tcaacgggaa acgtcgggga taatccaacc ctttcatcgc accaagttgc ggaacagcag
     5041 ctaacagcgc acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg
     5101 tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc
     5161 ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta
     5221 attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca
     5281 gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag
     5341 agagttgatg cggataacgt gaaagaatgg tttgtgcctc aggaatgcgt acctgatcca
     5401 gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg
     5461 attcggcaat ctgttcaccc acagtaaata ccgggttcag cgatgtcatc ggctcctgaa
     5521 atatcatcgc catatccgca ccgcgaacat ggcgcatttg tgcagcgctc tgctcgctaa
     5581 gttcaatcac ttcgcgactg cgccgctgca acagcatttt atcgcactgt actaaaccgc
     5641 ccgcctgttc caacaggcgc atcaatgcca acgcagtcac tgacttaccg gagccggatt
     5701 cgccaacaat tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag
     5761 ctattttctg ctggtcctgc ataaaggcaa tattcagatt ttcaaccgcc agcacattac
     5821 cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg
     5881 gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc
     5941 gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt
     6001 ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac
     6061 gcggcgatgt catatgccgc cagcgcgcgg atgaagactt cgcccgtgcc gatacaagaa
     6121 accgccacac tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact
     6181 cgtccgggta atttattggt cattccgcct gtggacgtgg ctgccgccaa attgccgtct
     6241 aaatccaacg ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg
     6301 ctatggtcga ggacggttgc cccttccttg cgcgctgcca gtagttgttc ataacgcaaa
     6361 ggcgtggaga aaatctccgg cgagacgcgc tccatgccac gagcaaacgc aaaattttct
     6421 gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga
     6481 accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc
     6541 atcacacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg
     6601 ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt
     6661 tcgcccgctt ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcaatgtag
     6721 cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt
     6781 gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata
     6841 aatatcagaa tttttatgaa ttactcattc aatttttgaa tatagaaaga tggatatggc
     6901 atgtaaaggc aggagccaac gctacgcccg aggtggcata cagtctgtgg cgtttttctg
     6961 acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg
     7021 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgtcaccc cactgaccgc
     7081 ccaggaaacg ctgccactgg tacagtgttt tggtcgtatt ctggcgagcg atgtcgtttc
     7141 gccgcttgat gttccggggt ttgataactc cgcaatggac ggctacgcgg tgcgtttagc
     7201 cgatattgcc tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta
     7261 tcatggtgaa tggcctgcgg gcacctgcat tcgtattatg accggtgcgc cggtgccgga
     7321 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
     7381 tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
     7441 tgcggttgtt ttcccggcgg gaactcgcct gactaccgca gagctgccag taattgcttc
     7501 actggggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg
     7561 tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg acaccaaccg
     7621 tctcgccgta cacctgatgt tagaacagct gggttgcgag gtaatcaact tagggattat
     7681 ccgcgacgat ccccatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt
     7741 agtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga
     7801 agagctgggg gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcgtt
     7861 cggtaaactc agcaatagct ggttctgcgg cctgccgggc aacccggttt cagcgacgct
     7921 gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg
     7981 cctgcccgcg cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct
     8041 tgatttccag cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac
     8101 cggacatcag ggttcacata tatttagctc ctttagtctc ggcaactgct ttatcgtgct
     8161 ggaacgcgat cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc
     8221 gttgttcgga ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg
     8281 caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg
     8341 ctgatcgtcg gcctcggtgg actcggctgc gcagcttcgc tgtatctggc aagcgccggt
     8401 gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag
     8461 acactgcaca gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg
     8521 acgcggatca acccacatat cgcgattacg ccagtcaatg cactgctaga tgacgcagaa
     8581 cttgcagcat tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta
     8641 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca
     8701 attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc
     8761 tgccttagcc gtttgtttgg cgagaacgca ttaacctgcg tagaagcagg cgtgatggca
     8821 ccgttgatcg gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt
     8881 tatggcaaac cgaccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc
     8941 gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca
     9001 ggcctgataa gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg
     9061 tgaacgcctt atctggccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg
     9121 cactccgcca cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc
     9181 tgccaaacgc tccctgccag tcctgctcaa acttcgccac agcggcttcc accgccggat
     9241 agctaatcat ctgttgtgcc acatccagtg gcagagtaat tgattcacat cctgccagta
     9301 agcagtccag cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg
     9361 catgcatttt caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag
     9421 catcaatacg attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcaacc
     9481 cttgtgctgc gccatatacc gcggttccca gcgttggaat cccttccgct tttaacatct
     9541 taatagctgc cagcccctcg gcggtcaccg gaactttcac cacgatatcc gcaataatag
     9601 aacgcaattt acgtgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa
     9661 acagacgccc ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt
     9721 ttttacccgc ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg
     9781 acagcgcctt caccgcaaca acgtctgaag tatccagata cagttccata accatcctca
     9841 aaatgtactt tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca
     9901 catcaatcta actttcattc gaaagtattt taatgtttga acgaaacaga gaggcgacta
     9961 tgattttcaa tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat
    10021 ttcttaaagg ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgca
    10081 cgcaggatct gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg
    10141 ccgcgccgga agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa
    10201 ccccggagca tctgacggcg ttaaccgact gctgtccgac acaggcatta accgtgtgtg
    10261 gtgaagtgaa aagcgttgag gagatcatga cgaccgttct gcgcgataaa ccgttttacg
    10321 atcgcagcgg cggcggttta acgctttcgg gtggtgagcc ctttatgcag ccggaaatgg
    10381 cgatggcgct actgcaagcc agccacgagg caggcattca tactgcggta gaaacctgtc
    10441 tgcatgtgcc gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg
    10501 atttaaaaca cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccagag
    10561 tgctggataa cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc
    10621 tgattcaggg ctttaatgcc gacgaaacct ctgtaaaagc tattaccgat tttgccgccg
    10681 acgagctgca cgttggcgaa attcatttcc tgccctacca cacgctaggc atcaacaaat
    10741 atcacttact taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagaactgc
    10801 tcgactttgc ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac
    10861 aaccatgacc acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc
    10921 gctggtgcat attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat
    10981 gtatcaacaa catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca
    11041 cctggcgaat cgcaccatct ggatcaaaca cgatgagttg atcattggca accaggcaag
    11101 cgaagttcgc gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat
    11161 tgatgatctg gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgcgt
    11221 tctgcatgaa gtgtgcccgt ggtggcgcgg tcagaccgta caggatcgct gctacggcat
    11281 gtttaccgat gagcaaaaag gtctgctggc gaccggaatc attaaagcgg aaggcaatat
    11341 gacctccggc gatgcgcacc tggcggtgaa tttcccgctg ctgctggaaa aaggtcttga
    11401 tggtctgcgc gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tactggaaga
    11461 tttgcacggc gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagcgaaca
    11521 cattgaacgt tttgcagccc tggcgcgtga aatggccgcg accgaaaccc gcgaaagccg
    11581 tcgcgatgaa ctgctggcga tagcagaaaa ctgcgatctt atcgcccacc agccgccgca
    11641 gactttctgg caggcgctgc aactgtgtta cttcatccaa ttgatattgc agatcgaatc
    11701 taacggtcac tcggtatcgt ttggtcgtat ggaccagtat ctctacccgt actatcgccg
    11761 cgacgttgaa ctcaaccaga cactggatcg cgaacacgcc atcgaattgc tgcatagctg
    11821 ctggctgaaa ctgctggaag tgaacaagat ccgctccggc tcacactcaa aagcctctgc
    11881 gggaagtcct ctgtatcaga acgtcaccat tggcggacaa actctggttg atggtcaacc
    11941 aatggacgcg gtgaatccac tctcttacgc tatcctcgaa tcctgcggtc gcctgcgttc
    12001 cactcagcct aacctcagcg tgcgttacca tgcaggaatg agtaatgatt tcctcgacgc
    12061 ctgcgtacag gtgatccgtt gcggcttcgg gatgccggcg ttcaacaacg acgaaatcgt
    12121 gatcccggaa tttattaaac tcggtattga accgcaggac gcttacgact acgcagcgat
    12181 tggttgtatc gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt
    12241 tatcaacttc gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg
    12301 caaagtgttc ctgccacaag aaaaagcgtt gtcggcaggc aactttaaca atttcgatga
    12361 agtaatggac gcgtgggata cgcaaatccg ttattacacg cgtaaatcaa tcgaaatcga
    12421 atatgtcgtc gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt
    12481 ggatgactgt attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt
    12541 ttctggcctg caggttggca ttgccaacct cggcaacagc ctggcggcag tgaagaaact
    12601 ggtatttgaa cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt
    12661 cgatggcctg actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg
    12721 caacgacgat gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga
    12781 actgaaacag taccataacc cgcgctacgg tcgtggtccg gttggcggca actattacgc
    12841 gggtacctcg tctatctccg ctaacgtacc gtttggcgcg caaactatgg caacaccgga
    12901 cgggcgtaaa gcccacaccc cgctggcaga aggcgcaagc ccggcctccg gtactgacca
    12961 tcttggccct actgcggtca ttggctcagt gggtaaactg cctacggcag cgattctcgg
    13021 cggcgtgttg ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca
    13081 gaaactgatg atcctgctgc gtaccttctt tgaagtgcat aaaggctggc atattcagta
    13141 caacatcgtt tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga
    13201 tctggtagtg cgtgtcgcgg gctattccgc cttcttcacc gcgctctctc cagacgcaca
    13261 ggacgatatc atcgcccgta ctgaacatat gctgtaatct cctcggcccg gcggcgtcgt
    13321 cgccgggcta aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt
    13381 gtgctctgcg tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac
    13441 ctatgagcgt aaaagttatc gtcacagaca tggacggtac ttttcttaac gacgccaaaa
    13501 cgtacaacca accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt
    13561 tcgttgtcgc cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg
    13621 atgagatctc ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt
    13681 tccacggcga actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata
    13741 agcaactcaa ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg
    13801 aagcatttgt cgcactgatg gcaaaacact accatcgcct gaaagctgta aaagattatc
    13861 aggagattga cgacgtactg ttcaagtttt cgctcaacct gccagatgaa caaatcccgt
    13921 tagtgatcga caaactgcac atagcgctcg atggcattat gaaacccgtc accagtggtt
    13981 ttggctttat cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac
    14041 tgaaacgctg ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg
    14101 cggagatgct gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta
    14161 aacaaatcgc ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc
    14221 aggcggtgct ggataacaca tcccctttta acagctgacc tctctaccgg agccgtgcgc
    14281 tccggttttc ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa
    14341 cttatttata acttatattg tcgcatgcaa atcagaatgg tttcgaatga aagttaatga
    14401 gaggtggtta tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct
    14461 atccgtcaga tgaagcacgc gctgcgggcg cagcttggcg acgtccagca gatctttaat
    14521 cagctaagcg atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc
    14581 gatgccgtct ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag
    14641 cagcgcgaac agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa
    14701 caagcgctag gctgggatca gtcgatgctg gactatctgg accgcaaccg ctttgacgag
    14761 gtctacaaag gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt
    14821 taccccatct actggtcgca ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat
    14881 gcgcagtcgt ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac
    14941 ccggatgtga gcgtcatcta ccctgaccgt atccgccgcc gtccgcccgg aacgacctcc
    15001 aaaggtcttg gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat
    15061 cagcgcgttt tcgccaacgt ctttaatggc aatctggcgc aatatgatcc ctggcatgcg
    15121 gcacatcgta cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt
    15181 cggacattcc aaggctggac agcgctctct aatatgctgc ctggtcaggg gctgctgcac
    15241 gttgtgccca ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg
    15301 ccggaggatg aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg
    15361 catccactgt taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc
    15421 tggtggcact gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc
    15481 aacgtgatgt acattcctgc ggcaccaatg tgcgagaaaa atcttgccta cgcgcacaag
    15541 gtgaaggccg cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa
    15601 acaaactggg aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg
    15661 ggaatcgaca gctgagtgtc gtacagccct tcccgttcca cggcggtgtt cccttttcct
    15721 gggcgaaccc gccgcactga ctcccgcacc gccaatcgtc cgtgcagcag cagcgagccc
    15781 accggagcat ccgggcgaat cagccgctgt tgcaacattt gcaccgcttc attgcctagc
    15841 tcatcgcgtg gcacatgcac agccgtcagg ggaacatcct cgattgccgc aaggttaaag
    15901 ccgtcgatac tcatcaccga gacatcctgt ggtacgcgca gaccacgacg atgtagcgca
    15961 tttaccgttc ccgccgccat aaaatccccg ccgaccagaa acgccgtcgg caacgtcttg
    16021 ccattggcgg catccagcca cgctccgacc ttttcttccg cttctttagc gctaaaactg
    16081 ctgatattca gcaaatgacg gtcatggtga aaggccagat tatgctgacg ccatgattct
    16141 ttaatccctg caaaccgctg ctccatagtg tagcggcgca ggcacatcac attcatcacc
    16201 tcccggtgac ccatttcaaa cagatagcgg gcggcgaacg cgccaattaa ccgatgatcc
    16261 ggcgcaatcg ccgggaggcg cattcgttca tcacggcagt tgatcagcac acaaggctta
    16321 gcgagatcgg cggccagatc atggatgtgc ggatcgtcaa tccccagcag gatcgctgcc
    16381 tgcgtatctg cttcattcat ccgcgagaga aagagcgcgg gatcgctgtc aaactcctcc
    16441 agcgcgcagt aacgcagccg cacttcatgc ggcgataacg ctttgctgat gctctggatc
    16501 acacggtaat aaaagatatc agatcgttgg tcaaaagcgc gttgcggcgc gaaaataatc
    16561 aggttgttca gcaacatacg acctgctgcc atgccatcca tcacgcccaa ctcacgcgcg
    16621 caggccagca cctgttcgcg agcgcgtgca ctggtattcg ctttgccagc cagcacccgc
    16681 gaaaccgtac tcggcgatag cccggtacgt gcggcgatat cggcgatttt cagcttcttg
    16741 tccattttgt gatctgcctc taattcggaa atgacagaat tttcatgagc aaaaaagccc
    16801 attctgtctg gcattaaatc catttcagta atgtatatca gtcactcatg gaaaaacgtg
    16861 cacaaaaccc gctttctttc gcttttaggg tctcttactg tgaattggta gaacaacttc
    16921 catacaaaaa taatggaaaa acgaacaata aagactttat ttatcaaaaa gttaaaagtg
    16981 gtgtaacaac tccattctga cgttgccgca caaaccctat accattctgt ggagtgcata
    17041 tgagtcaggg tatcaataac gatatgactg ccagtaaacc gcgccatatc gtcaaaaatc
    17101 tgcgttggtg gatactggtc ctttttttgc tcggtgttac tgtcaactac atcacgcgta
    17161 actcgctggg tattctcgcc cccgaactga aaagcagcct agggattacc accgaacaat
    17221 attcctggat tgtaggtgct ttccagctcg cctataccct attccaaccg ttgtgcggat
    17281 ggctgattga cgtcattggc ctcaaactgg gctttatgat ctgcgcggga ctgtgggcac
    17341 tggcctgtct gatgcatgcg ggggcgggaa actggggaca tctggcgctg ttgcgcttct
    17401 ttatgggcgg tgcggaagcc gcggcaaccc cagcaaacgc caaaaccatc ggtgaatggt
    17461 ttccaaaatc agaacgcccg atcgccgcag gctgggcagg cgtcggtttt tccatcggtg
    17521 ccatgctggc cccaccgatt atctattttg cccacgcttc attcggctgg cagggtgcat
    17581 ttatgttcac tggcatcctg gcgatgctgt gggtggtgct gtggtgggcg ttctatcaca
    17641 gcccggataa gcatcctaac ctcagcaaag ccgagctgga ctttattcgc caggataacg
    17701 aagcgccccc ggtcaagtta ccctttctga ccgcactgaa aaccgtgggc aaaaataaac
    17761 gcttttacgg tatcgctatc ccggccttta tggcagaacc ggcgtgggcg gtgatgagtt
    17821 tctgggtgcc tctatacctg tccaaaacct atggcatgga cctgaagcag atcgcattat
    17881 tcgcctggtt gccgttcctc gccgctgatc ttggcagtat tgccagcggc tatctgacca
    17941 aactttacac ccgcctgttt gggctgagtc gtgtgaactc cgttgtcgca tcatcggtca
    18001 gtggcgcatt cttgatggtt tcgctggcgg tgatggcctt taccaataac ccgtatatcg
    18061 ccatcgtcct tatctccatc ggcggcttcg gtcaccagat catctcctgc atgctgagcg
    18121 cactggtggt ggaatccttc gacaaaggtc agatggccac tgttaacggc atgcgcggtt
    18181 ctgccgcgtg gatcgcaagt ttcctgttct ccctgattat cggggttacc gctgacaaaa
    18241 tcggctttaa ccccctgttt atcgccatgg gcttctttga cctgattggc gctttcttcc
    18301 tggtcacctt tattgctgaa cgtcgcgcaa aacgcgcctg agagtggatg taaacatgaa
    18361 aacgctgaaa aactggaccc tgcacaaaca gtctgcccac cacgttgaac tgctggtcga
    18421 tggccagcat atcctgtgcc tgtacgtgct ggaagaaaat ctgttccgcg tgctgttaaa
    18481 acgcaaaggc gagctggcgc tagatcgcac ctggagcatt gctccggcgc aggatgtgcc
    18541 atgggaaggg cgcagccgcg aagatctcag cggctttagc ctgcccgcgt ggacagtgat
    18601 ccagcacgac gacaccgtgg ttatcgccac cgagtcactg cgtgcaaccg ttcaccagcc
    18661 gctgtggctg gagtggcact atcgtaacaa cgaaggcgaa tggctgccgt tggtgaatga
    18721 ccgcccgacc agcgcttatc tgctgaatgc ccacggcgat ggcgtggcac actatcagag
    18781 ccgtcgtaaa gatgaacgct tttatggcct cggagataaa gccggcgatc tgcaacgcaa
    18841 cggtaaacgt tacgagatgc gtaacctcga cgcgatgggc tacaacgccg ttagtactga
    18901 cccgctgtac aaacacattc cattcaccat cacccgccgc gatgacatta gcttcggcct
    18961 gttttatgac aacctcagca gctgctggct ggatctgggc aacgagatcg acaactacca
    19021 caccgcctat cgccgctggc aggcggaagc gggtgatatc gactactacc tgtttaccgg
    19081 cgaacgcgta cttgatatca ccaaagcctt cgtacgcctg accggaaaaa ccctgttcgg
    19141 accgaaatgg agcctgggct acagcggttc aactatgcac tacaccgatg cgccggacgc
    19201 acaaaatcag ttgatgaact ttatccgcct gtgtgaagaa catgcaattc cctgcgattc
    19261 gttccagctc tcttccggtt atacctcaat taacggcaaa cgctatgtgt tcaactggaa
    19321 ctacgacaaa gtgccgcagc cgaaagtgat gagccaggca tttcacaatg ctggtttgca
    19381 cctggccgcc aatatcaagc cgtgcctgtt gcaggatcac ccacgctata acgaagtggc
    19441 agaaagagga ctgttcatcc gtgattcaga aacagatgaa ccagaacgtt ccagcttctg
    19501 ggatgacgaa ggttctaacc tcgagtttac taatccgcag accatccagt ggtggcagga
    19561 aggcgtgacc acacagcttc tggagatggg tatcgactcc acctggaacg acaacaacga
    19621 attcgaagta tgggacggtg aagcacgctg tcacggcttt ggtcagacga tcgcgatcaa
    19681 acatattcgc ccagtgatgc cactgctgat gatgcgagcc tcgatggaag cccagcagcg
    19741 tttcgctccg gaaaaacgtc cgtacttgat ctcccgctcc ggctgcgcgg gaatgcagcg
    19801 ctacgtgcag acctggagtg gcgataaccg cactagctgg gacactctgc gctacaacac
    19861 ccgcatgggt ctggggatga gcctctccgg gctgtataac ctcggtcacg acgtcggcgg
    19921 tttctccggt gataagccgg acccggaact gtttgtacgc tgggtacaaa acggcgtgat
    19981 gcatccgcgc tttacgattc actcatggaa cgatgaccat accgtaaacg aaccgtggat
    20041 gtatccgggc gtcactccgg cgatccgcag cgctattgag ttgcgctatc gcctgctgcc
    20101 ctacttttat accctgatgt ggcaggcata cgccgatgat gaaccaatgc tacgtccgac
    20161 tttccttgac cacgagcacg acgttcagac ttttgaagag tgtgatgact tcctgcttgg
    20221 tcgcgatatt ttggttgcca gcgtcgtaga gcaaggcgag cgccagcgcc gtgtatggct
    20281 gccagataac gaaactggct ggtacgattt ctacaacggc gaatggttca gtggcggtca
    20341 gtggatcacc ctcgacgcac cgctggagaa actgccgctg atggttcgtg ccggggctgg
    20401 tctaccgctc agcaaacgca tcacctatgt gagcgcagaa caagacgata cccgcgaact
    20461 gaaactgttc ccgctgaaag gtgtgggcac aacgtccggc ctgctgtttg aagacgatgg
    20521 cgaaagctgg ggctaccaaa ctggtaatgc gctttgggtg gaatgggaaa tggtttgcga
    20581 tggggcgacc atcaacctga aagtcaacgc gcgcggggat tatcgtcctg catggagtgc
    20641 actgaaagtg tcattaccat tggaagaaaa acgcacgctg ctggtcaatg gtgttgaagg
    20701 gagtgaatgg atgcgttaaa tcattaccgg ggccgggagg cgactgcacc ttacccggtc
    20761 cagccatcga atcctttacc tcagtatttc acgctgcagg aaggcagcaa acctgtaaac
    20821 cccgggagct taaataagta agtgaccggg gtgaacaggt gaagccaatg catctgccgc
    20881 atgaaagacg gcggggaatt aagcgtcgat ccctttacta cgcaggtaat cttcgtaatt
    20941 accgctaaag tcgatcgcgc gttccggggt gatttccaga atgcgggtcg ccagggagct
    21001 tacgaactca cggtcgtgtg aaacaaagat cagcgtgccc tgatacagtt ccagcgccat
    21061 gttcagtgac tcgatggatt ccatatccag gtggttggtc ggttcgtcca tgatcagaat
    21121 gttcggcttc tgcatcatta acttaccaaa cagcatccgc cctttttccc caccggaaag
    21181 cactttagct ggctttttga tgtcgtcctg gctgaacagc aaacgaccga gaatgctgcg
    21241 taccgcctgc tcgtcatcgc cttcctgctt ccactgactc atccattcga acacggtcag
    21301 atcattttca aactcatatt cgtggtcctg ggcgtagtaa ccaatgcgcg cgttttcaga
    21361 ccattttacg gtgccgctgt ccggttgcag atcgcccacc agcgttttca gcagcgttga
    21421 tttaccgacg ccgttggtac ccagtaccgc aagtttttca ccgacctcca gcagcaggtt
    21481 gagattttta aacagtggac cgttatcaaa ccctttggtc agaccttcca cttccagcgc
    21541 gttacggaac agtttcttat cctgttcaaa acggatgaac gggttctgac ggctggaggc
    21601 tttcacctct tccagtttga ttttatcaat ctggcgcgcg cgcgaagttg cctggcgaga
    21661 tttcgaggcg ttggcgctaa agcggctaac gaaagattgc aactcagcaa tctgcgcttt
    21721 cttcttggcg ttatcggcca gcagacgttc acgcgcctgg gtcgccgccg tcatgtactc
    21781 atcgtagtta cccggataaa cgcgcagctc gccgtaatcc agatccgcca tgtgggtaca
    21841 gaccatgtta aggaagtgac ggtcgtgcga gatgatgatc atggtgctgt cacgctcgtt
    21901 cagcacctgt tccagccagc gaatggtgtc gatgtcgagg ttgttggtcg gttcgtcgag
    21961 caggagaatc tccggatcag caaacagcgc ctgcgccaga agcacacgca gtttccagcc
    22021 aggagcaact tcactcatcg ggccgtagtg ctgttccact ggaattccca cgccaagcaa
    22081 cagttcaccg gcgcgagctt ccgcagagta accgtccatt tcgccgtatt taacttccag
    22141 atcggccact ttatagccgt cttcctcact catttccggc aaagcataga tgcggtcacg
    22201 ctcctgcttc acttcccaca actctttatg ccccatgatc accgtatcca gcacagtgaa
    22261 ctcttcaaag gcaaactgat cctgacgcag cttaccaatg cgctcgttgg gatcgaggga
    22321 aacgttaccc aaagtcggct ctaagtcgcc gccgaggatc ttcataaagg tggatttacc
    22381 actaccgttc gcgccaatca ggccgtaacg gttgccgccg ccgaatttga cggaaatgtt
    22441 ttcaaacaac ggcttactgc cgaactgcat ggtgacgtta ctggaaacta acacaggcgt
    22501 atcctgaaaa gagatatgac aaaccgcgca ttatgccata agtctgaaag aagatcctgc
    22561 tcctcgctta aggtctacac tcaagccaaa ggcaaaaaat gtgatttcgt acacatctga
    22621 tttcactgtg agctggaatg aacttataat gcgcttccaa tactctaata ttctcaaccc
    22681 aatggcctgc caggcacaaa atctcgctta acatgaatat gaaattgaaa acattattcg
    22741 cagcggcctt cgctgttgtc ggcttttgca gtaccgcctc tgcggtaact tatcctctgc
    22801 caaccgacgg gagtcgcctg gttggtcaga atcaggtgat caccattcct gaaggtaaca
    22861 ctcagccgct ggagtatttt gccgcggagt accagatggg gctttccaat atgatggaag
    22921 cgaacccggg tgtggatacc ttcctgccga aaggcggtac tgtactgaac attccgcagc
    22981 agctgatcct gccggatacc gttcatgaag gcatcgtcat taacagtgca gagatgcgtc
    23041 tgtattacta tccgaaaggg accaacaccg ttatcgtgct gccgatcggc attggtcagt
    23101 taggcaaaga tacgcctatc aactggacca ccaaagttga gcgtaagaaa gcaggcccga
    23161 cctggacgcc gaccgccaaa atgcacgcag agtaccgcgc tgcgggcgaa ccgcttccgg
    23221 ctgtcgttcc ggcaggtccg gataacccga tggggctgta tgcactctac atcggtcgcc
    23281 tgtacgctat ccatggcacc aacgccaact tcggtatcgg cctgcgtgta agtcatggtt
    23341 gtgtgcgtct gcgtaacgaa gacatcaaat tcctgttcga gaaagtaccg gtcggtaccc
    23401 gcgtacagtt tattgatgag ccggtaaaag cgaccaccga gccagacggc agccgttata
    23461 ttgaagtcca taacccgctg tctaccaccg aagcccagtt tgaaggtcag gaaattgtgc
    23521 caattaccct gacgaagagc gtgcaaacag tgaccggtca gccagatgtt gaccaggttg
    23581 ttcttgatga agcgattaaa aaccgctccg ggatgccggt tcgtctgaat taatcttcaa
    23641 aacttaaagc aaaaggcgga ctcataatcc gcctttttta tttgccagac cctagttggc
    23701 cgggagtata actaacaaaa catatcccac caacgccgcc cacaacagca tcggtattga
    23761 atagagatgg aaacgccacc agatgcggcg atcgttcgcc atacgtagcg caatcaaatt
    23821 tgccagcgat ccgggtaata acccaaagcc acctacgttt accgcccata ccagtaataa
    23881 agacggcggc acatagttca gcaacaatat ggtactcggc acgttactga tcacctgcga
    23941 taaaccgatt gccgttaacc ataacccggg ttcagataga tgactcacgt tacccaacac
    24001 gccttgcaac gctggaagct gggtcagtaa atggacgtcg ataaacatcg ccataaacac
    24061 cagcagcagc gtccagtcca cactgagcac cacgcgacgt gccagcagcg caaagcctgc
    24121 cgccacaatc accagtcccc acagctcttg tttgaactcc agcgccgtca gaaagacgat
    24181 atacagcccc agacaactcc acaccagccg cggtttccac tccggtgttt gcacccccgt
    24241 atggtattgc agcgcctttc cagggaaaca acaccagcac aggagcagga gcgtcagcat
    24301 cattgcgcca gccagcggtg ccatttgggc aataaatccg gcaaacgaaa gaccagaacg
    24361 tccccagata agaatatttt gcgggttgcc aattggcgtc agtagcgaac cagcgttgac
    24421 tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc tcacacagtc tttttagcgt
    24481 gatagtcagc ggaacaacaa taaacagcgc gacatcgttg gtcagaaagg tagaaagcag
    24541 cgccgccgcc agcaccataa acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat
    24601 tttgcgcccc agcacatcaa aataaccgct taactccaca cctttggtca gcagcatcag
    24661 gccgcttaag gtgatgatgg tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa
    24721 gggcacgaaa aagcttaatc cgataccaac aagaattaat aactgaaaaa aacgatcgcc
    24781 ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt gtttctgggt aaacaaacga
    24841 aaagcgtcga gcgtctcttc actaacatgg tgctccatgc cttccgcgtc gcgacgggcg
    24901 atttccggac tgacgcccaa caccaacaag aaattttcga ctatctgatg tcgctcgcgg
    24961 ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca cgccacgcca ggggatcatt
    25021 tcaatcagcc ccatggttgc cagccgctta agcattttag ccaccgtcgg ttgcgaaact
    25081 cccagacgag cagccatgtc cacctgacga gcttccccca cttccctgat caagtcagaa
    25141 atcagctcaa cgtaatcatc aataagctcg cgccgatgcg cctctctgac ctggcggaac
    25201 ccttcaacgt gctcttccac gttcactaac tgcgtcactt tttttgctgt tggcgtacct
    25261 gcgcgacgac tcattgtgct tcctcattat ggtgacgcat acatagcgtt ctcatttcgt
    25321 gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt aacgttttag caatagctac
    25381 ataatatagc ctgtgctata tctgtatgta atgcaatcat ccctcaagga tcgacgggat
    25441 tagcaagtca ggaggtctta tgaatgagtt caagaagtgt atgcgcgtgt ttagtcattc
    25501 tccctttaaa gtacggttaa tgctgctctc tatgttgtgc gatatggtca acaacaaacc
    25561 gcagcaagat aaaccttccg ataaatagcg gcgtcgcggt acgccgcttc actcctgctt
    25621 ttatgcaggc ataacgcgtt ttggtctgaa aaaccccact ttttgtcgga tttgcaatcc
    25681 ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg gcggctcgct ctatcttctt
    25741 gcagccctac gtatattgcg gctcgcggat gcggacccct ttcccctctt cacgcactct
    25801 tgcaggtatt gacccttgac gccagggtaa gcacatggcg tttgttacga tagtggcata
    25861 tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg ggtatttagc ccgtggactg
    25921 cgttctactt tttacagtcg ctattaatta acctcggctt aggttacccc ttcagtttgc
    25981 tctacaccgc tgcgtttacg gctattttgc ttttgctatg gcgaacattg cctcgcgtac
    26041 aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg ttatttccct tttgctcagg
    26101 cctacggcgc gcctaatttc aatacattgc tggcattgca ctccaccaat atggaagagt
    26161 cgaccgaaat cctgacgatt tttccgtggt acagctacct ggtcggctta tttatttttg
    26221 cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct
    26281 tcgacagcct gtgtctggta ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc
    26341 tggcctgggg tggcgtattt aaactgaaag atactggcta tccggtattt cgttttgcta
    26401 aggatgtcat cgtcaataat aacgaggtga ttgaagagca agaacggatg gcaaaacttt
    26461 ccggaatgaa agatacctgg acggtcactg ccgttaagcc gaagtatcag acctatgtgg
    26521 tggtgatcgg cgaaagcgcg cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca
    26581 ataccccgtt tgccagcagc gttaacggtt tgatatttgc tgactacatt gccgccagtg
    26641 gctccacgca gaaatcactt ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt
    26701 ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt ccagacctgg tggttttcca
    26761 accagggcca aatcggcgaa tacgatactg ctatcgccag catcgccaaa cgtgcagatg
    26821 aagtgtactt cctgaaagaa ggtaatttcg aagcagataa aaacacgaaa gacgaagcgt
    26881 tactggatat gaccgctcaa gtgctggcgc aagagcactc gcaaccgcag ctgattgttc
    26941 tgcatctgat gggctcgcat ccgcaggcct gcgacaggac acaaggcaaa tacgaaacct
    27001 ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat gacgcaaacg gacgatttac
    27061 tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag cttctctctg gtttactttt
    27121 ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg
    27181 ataaatatca gcaaaatttc caggtgcctt ttatggtcat ttccagcgac gataaagcgc
    27241 atcgggtgat taaagcccgc cgctcagcca atgacttctt aggctttttc tcgcagtgga
    27301 cgggaattaa agcgaaggaa ataaatatca aatacccgtt tatatctgag aagaaagccg
    27361 ggccgatata catcaccaac ttccagttac agaaggtgga ttacaaccat ctcggaaccg
    27421 atattttcga cccgaaacct taaaacaaaa aatccgcccc gagaggcgga ttttttatat
    27481 caccaaagtg attagaagcg gtaaccaaca ccggcaatcc aggtgcctac gtcaacgcta
    27541 cgaatacggc tctgctcgta agagaagtcc agagcaacgt tttccatcgg gttgaactgc
    27601 agaccagcac cgtaggagaa accgtagtcg ctggtgtcgt gtttgtaggt cgggtattca
    27661 gtggtctgga atttaccata acccaccccc actacaccgt agatgcttgc ccagtcgtta
    27721 atgcggtaag ccggaccagc agtgatgccg tagtactggt ttttgttgta gtcaccagag
    27781 cttgcagtac ggcttttctc ggtgtaagtg aaagaaccga tcacacccag cgggctgttg
    27841 tcttcttcat agcggtattt caggttgaaa ccgcccattt tgttcatttg gccctgagcg
    27901 tcgctctgtg cgtaaccgcc agttacggta gaagtcgcag ctacggaagt acctgcggtg
    27961 aaagccagaa ctgcggccag tgctgaaaga catgcaattt ttttcataac cacctcaaat
    28021 gtgattcaaa taagtcctaa gttttaaata tatcaaaaat taatgggaaa ctcttcgcga
    28081 tttgtgatgt ctaacgggcc atttcatgta acagaacgtt tccatacacc gctatccatc
    28141 taaatttaaa tcactttttc agagaactgc gtaagtatta cgcatgtttt ccctgtcatt
    28201 catccagatt attcctaatc accagactaa tgattccatc aatcctggcg cattttagtc
    28261 aaaacggggg aaaatttttt caacaaatgc tcaaccagca ttgggtatat ccagtacact
    28321 ccacgcttta cttaagtcta gatatttgtg ggagaaagga tgcctggttc attacgtaaa
    28381 atgccggtct ggttaccaat agtcatattg ctcgttgcca tggcgtctat tcagggtgga
    28441 gcctcgttag ctaagtcact ttttcctctg gtgggcgcac cgggtgtcac tgcgctgcgt
    28501 ctggcattag gaacgctgat cctcatcgcg ttctttaagc catggcgact gcgctttgcc
    28561 aaagagcaac ggttaccgct gttgttttac ggcgtttcgc tgggtgggat gaattatctt
    28621 ttttatcttt ctattcagac agtaccgctg ggtattgcgg tggcgctgga gttcaccgga
    28681 ccactggcgg tggcgctgtt ctcttctcgt cgcccggtag atttcgtctg ggttgtgctg
    28741 gcggttcttg gtctgtggtt cctgctaccg ctggggcaag acgtttccca tgtcgattta
    28801 accggctgtg cgctggcact gggggccggg gcttgttggg ctatttacat tttaagtggg
    28861 caacgcgcag gagcggaaca tggccctgcg acggtggcaa ttggttcgtt gattgcagcg
    28921 ttaattttcg tgccaattgg agcgcttcag gctggtgaag cactctggca ctggtcggtt
    28981 attccattgg gtctggctgt cgctattctc tcgaccgctc tgccttattc gctggaaatg
    29041 attgccctca cccgtttgcc aacacggaca tttggtacgc tgatgagcat ggaaccggcg
    29101 ctggctgccg tttccgggat gattttcctc ggagaaacac tgacacccat acagctgctg
    29161 gcgctcggcg ctatcatcgc cgcttcaatg gggtctacgc tgacagtacg caaagagagc
    29221 aaaataaaag aattagacat taattaaatt tacatttctg catggttatg cataaccatg
    29281 cagaatttct cgctactttt cctctacacc gtctttatat atcgaattat gcaaaagcat
    29341 atttattccg aaaattcctg gcgagcagat aaataagaat tgttcttatc aatatatcta
    29401 actcattgaa tctttattag ttttgttttt cacgcttgtt accactatta gtgtgatagg
    29461 aacagccaga atagcggaac acatagccgg tgctatactt agtctcgtta attactggga
    29521 cataacatca agaggatatg aaattatgag taccgctaaa ttagttaaat caaaagcgac
    29581 caatctgctt tatacccgca acgatgtctc cgacagcgag aaaaaagcaa cagtagagtt
    29641 gctgaatcgc caggttatcc agtttattga tctttctttg attaccaaac aagcgcactg
    29701 gaacatgcgc ggcgctaact tcattgccgt acatgaaatg ctggatggct tccgcaccgc
    29761 actgatcgat catctggata ccatggcaga acgtgcagtg cagctgggcg gtgtagctct
    29821 ggggaccact caagttatca acagcaaaac cccgctgaaa agttacccgc tggacatcca
    29881 caacgttcag gatcacctga aagaactggc tgaccgttac gcaatcgtcg ctaatgacgt
    29941 acgcaaagcg attggcgaag cgaaagatga cgacaccgca gatatcctga ccgccgcgtc
    30001 tcgcgacctg gataaattcc tgtggtttat cgagtctaac atcgaataaa tccatcgctg
    30061 atggtgcaga actttagtac ccgataaaag cggcttcctg acaggaggcc gttttgtttt
    30121 gcagcccacc tcaacgcact tatttagtgc atccatctgc tatctccagc tgattaagta
    30181 aattttttgt atccacatca tcacacaatc gttacataaa gattgttttt tcatcaggtt
    30241 ttacgctaaa taatcactgt gttgagtgca caattttagc gcaccagatt ggtgccccag
    30301 aatggtgcat cttcagggta ttgccctata aatcgtgcat cacgtttttg ccgcatctcg
    30361 aaaaatcaag gagttgcaaa actggcacga ttttttcata tatgtgaatg tcacgcaggg
    30421 gatcgtcccg tggatagaaa aaaggaaatg ctatgaagtc tgtattaaaa gtttcactgg
    30481 ctgcactgac cctggctttt gcggtttctt ctcatgccgc ggataaaaaa ttagttgtcg
    30541 cgacggatac cgccttcgtt ccgtttgaat ttaaacaggg cgataaatat gtgggctttg
    30601 acgttgatct gtgggctgcc atcgctaaag agctgaagct ggattacgaa ctgaagccga
    30661 tggatttcag tgggatcatt ccggcactgc aaaccaaaaa cgtcgatctg gcgctggcgg
    30721 gcattaccat caccgacgag cgtaaaaaag cgatcgattt ctctgacggc tactacaaaa
    30781 gcggcctgtt agtgatggtg aaagctaaca ataacgatgt gaaaagcgtg aaagatctcg
    30841 acgggaaagt ggttgctgtt aagagcggta ctggctccgt tgattacgcg aaagcaaaca
    30901 tcaaaactaa agatctgcgt cagttcccga acatcgataa cgcctatatg gaactgggca
    30961 ccaaccgcgc agacgccgtt ctgcacgata cgccaaacat tctgtacttc atcaaaaccg
    31021 ccggtaacgg tcagttcaaa gcggtaggtg actctctgga agcgcagcaa tacggtattg
    31081 cgttcccgaa aggtagcgac gagctgcgtg acaaagtcaa cggcgcgttg aaaaccctgc
    31141 gcgagaacgg aacttacaac gaaatctaca aaaaatggtt cggtactgaa ccgaaataat
    31201 aacgctacac ctgtaaagcg cactggcagt tccctctccc ctctggggag aggattaggg
    31261 tgaggggcgc aaacccgctc cggggccatt aattaccctg aatttgatta tttacaccac
    31321 ggtaacagga acaacatatg cagtttgact ggagtgccat ctggcctgcc attccgcttc
    31381 tgattgaagg tgccaaaatg accctgtgga tttcggtcct cggtctggct ggcggtctgg
    31441 taatcggatt gctggcaggt tttgcacgca ccttcggtgg ttggatagcc aaccacgtcg
    31501 cgctggtctt tattgaggtg atccgcggca cacctatcgt cgtccaggtg atgtttattt
    31561 atttcgccct gccgatggcg tttaacgact tacgcatcga cccatttact gcggcggtgg
    31621 tcaccatcat gatcaactcc ggcgcgtaca ttgcggaaat cacgcgtggt gcggtgctgt
    31681 ctattcacaa aggttttcgt gaagcaggac tggcgctcgg tctttcacgt tgggaaacca
    31741 ttcgctacgt cattttaccg ctggcactgc gtcgtatgct gccgccgctg ggtaaccagt
    31801 ggatcatcag cattaaagac acctcgctgt ttattgtgat cggcgtggcg gaactgaccc
    31861 gtcaggggca agaaattatt gccggtaact tccgcgccct tgagatctgg agcgccgtgg
    31921 cggtgttcta tctgattatt accctggtgc taagctttat tttgcgtcgt ctggaaagaa
    31981 ggatgaaaat cctgtgattg aatttaaaaa cgtctccaag cactttggcc caacccaggt
    32041 gcttcacaat atcgatttga acattgccca gggcgaagtc gtggtgatta tcgggccgtc
    32101 cggttccggt aaatcgaccc tgctgcgctg catcaacaaa ctggaagaaa tcacctccgg
    32161 cgatctgatt gtcgatggcc tgaaggttaa cgatccgaaa gttgacgagc gcctgattcg
    32221 ccaggaagca ggtatggtgt tccagcagtt ttacctcttc ccgcatctga cggcgctgga
    32281 aaacgtcatg tttggtccgc tacgcgtgcg tggcgcgaac aaagaagagg cggaaaaact
    32341 ggcgcgtgag ctgctggcga aagtcggtct ggcagaacgt gcacatcact acccttccga
    32401 actttctggt ggtcaacagc agcgtgtggc gattgcccgc gcgctggcgg tgaagccgaa
    32461 aatgatgctg tttgatgaac cgacttccgc tcttgacccg gaactgcgcc atgaagtgct
    32521 gaaggttatg caggatctgg ctgaagaagg gatgacgatg gtgatcgtga cccacgaaat
    32581 cggttttgcc gagaaagtag cttcgcggct gatctttatc gacaaaggcc ggattgcgga
    32641 agatggcgat ccgcaggtat tgatcaagaa cccgccgagc cagcgcttgc aggaattttt
    32701 gcagcacgtc tcttaacttc tctgcctccc tttcacccga aagggaggca caccagattc
    32761 ctctcattta aaatcgcccc tcccccagca tctatactta tctttttgct ctgttttctc
    32821 actggaggag tcatgcggtg gatcctgttc atcctcttct gcctgctggg cgcacctgcc
    32881 cacgcggtat ccatacccgg cgttacaacc acaacgacaa cggactcaac gactgaaccg
    32941 gccccggaac cggatatcga acaaaaaaaa gcggcctatg gcgcactggc ggatgtgctg
    33001 gataacgaca cctcgcgtaa agaattgatc gaccagttgc gtactgtcgc cgcgacgccc
    33061 cctgcggaac cggtcccgaa gatcgtgccg ccgacgctgg ttgaagagca aaccgtgctg
    33121 caaaaggtca ccgaagtcag ccgccattat ggtgaagccc tttccgcccg cttcgggcaa
    33181 ctttatcgca atatcaccgg ctccccgcat aagccattta atccacaaac cttcagcaat
    33241 gcgctgagcc atttttcaat gttagcggta ttagtgtttg gtttttactg gctgattcgc
    33301 ctgtgcgcac tgccgctgta tcgcaaaatg ggtcagtggg cgaggcaaaa aaatcgtgag
    33361 cgcagcaact ggttgcagct tccggcgatg attatcgggg cgtttattat cgacctgctg
    33421 ttactggccc tgacattatt tgtcggccag gtattaagcg acaacctgaa tgcaggcagt
    33481 cgcaccatcg ctttccaaca aagtttgttt ctcaacgcct ttgccctgat tgaatttttc
    33541 aaagccgtac tacgcctgat tttttgccca aacgtggcgg agctgcgccc gttcacgatt
    33601 caggacgaga gcgcccgtta ctggagtcgt cgcctgagct ggttaagcag tttgataggt
    33661 tatggcctga ttgtggccgt gccgattatc tctaatcagg tgaatgtaca gataggtgcg
    33721 ctggcgaacg tcatcattat gctgtgcatg accgtctggg cgttgtacct gatctttcgt
    33781 aataaaaaag agattaccca gcatttgctc aactttgcgg agcattcgct ggcctttttc
    33841 agcctgttta tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc ctattttatc
    33901 gtgctgtttt tcttttcgtt gttcgatccg ggcaacagcc tgaaatttat gatgggtgca
    33961 acggtgcgca gcctggcgat tattggtatc gcagcgtttg tttccggtat gttttcccgc
    34021 tggctggcaa aaaccatcac tctctcgcca catactcagc gtaactatcc agagttgcaa
    34081 aaacggctga atggctggct ttcggcggcg ctgaaaacgg cgcgtattct gaccgtctgc
    34141 gtggcggtaa tgttgctgtt gagcgcatgg ggattgttcg atttctggaa ctggctgcaa
    34201 aacggcgcgg ggcagaaaac cgtagatatc ctgatccgta tcgcacttat tctttttttc
    34261 tcggcggttg gctggacggt gctcgccagt ttgatcgaaa accggctggc ttcggatatt
    34321 catggccgcc cgctacccag cgcccgtacg cgtaccctgc tgacgctgtt ccgtaacgcg
    34381 ctggcggtga ttatcagtac catcaccatc atgattgtgt tgtcggaaat cggcgtcaat
    34441 atcgcgccat tgctggcagg tgccggggca ttaggtctgg ctatctcgtt tggttcgcaa
    34501 acgctggtga aagatatcat taccggggtg tttattcagt ttgaaaacgg catgaacact
    34561 ggagatttgg tgactatcgg gccgttgacc ggcacagtgg aacggatgtc gattcgctcc
    34621 gtgggcgtgc gccaggatac cggggcgtat cacatcattc cgtggtcttc gataaccacc
    34681 tttgctaact tcgtccgcgg tattggttcg gtagtggcaa actacgatgt tgatcgccat
    34741 gaagatgctg ataaagccaa tcaggcactg aaagatgcgg tagcggaatt aatggaaaac
    34801 gaagaaattc gcgggctgat tattggtgaa ccgaattttg ccgggattgt cggcttaagc
    34861 aataccgcgt ttacactgcg tgtttcgttc accacgctgc cactcaaaca gtggacggta
    34921 cgctttgccc tcgacagcca ggtgaaaaaa catttcgacc tggcgggcgt tcgcgcgcca
    34981 gtgcagactt atcaggtgct gcctactccg ggcgcgaccc cggctgaacc gttaccgccg
    35041 ggggagccaa cgctttaacg ctggcgattg acaaaacggc ggcgctgctc gtcgttcata
    35101 aaggtccagg caataaagcg actctgcttt tgcccctggg ccatctcttt ttttaccacc
    35161 ttcaccgcgc ccacgtcggt cagagcacga tacaacggcg gtaagttttc accacgagat
    35221 accagcgagg taaaccacat cacctgcttc gcgaagcctt tgctctcttc aatcatcttt
    35281 ttgataaagg cgacttcacc accttcacac cacaactcct gttgctgacc accaaagttc
    35341 agtgcatcgt ctttgttcag ccccaggtta cggcgtttac gctcactacc tgcccgcgct
    35401 gcagcggcgg aatcgtggaa tggcgggtta cacaaggtcg cgtcgtattg ctcgttttta
    35461 tggatgatgc cgttaaaaat tgccccactc tctttttgcc gacgcagacg aatggcgcgg
    35521 ttaagccccg gattagcact gataatcgcc tgcgcactgc ttaacgcctg gctgctggtt
    35581 tcactaccgg taaaacgcca gccatattca tgtacgccaa tcagcggata aatacagttc
    35641 gcaccaacgc cgatatccag aatgctggca ttcgctggaa ttgttccgct tgcttcagcc
    35701 agtaaatcgg caaggtgatg aatataatcc gcccggcccg gtactggtgg gcagagaaaa
    35761 ccgtcgggga tatcccagtt cgctacggcg taaaaatggg ccagcaacgc cttattgagc
    35821 gccttcaccg ccagcggatt ggcaaagtct acgctttgct ccccggcggg tgtaagcgtg
    35881 aggaattgcc tgagttcagg attgacctga caaagcgtgg cgagatcgta gcggctgtga
    35941 tgacggttgc gcggatgcaa ccccggtttc tgggcggaca tagcattctc ctttaagcag
    36001 cggcgtaaga tacccgcata acgcgacagg gtaaatatct ggctgcgttt atcattcccc
    36061 aaaaccttaa catggctatg gtttatggca attcatcata aaaatcagca ttatgcatgt
    36121 cattaatttg tcttattttg taaaaatata tcgttcaaaa aataagcaca tctctgtcaa
    36181 ttttttgcgt catttgcatc gcgcaggcga cctacactta tcaggcacta cccgttgtat
    36241 ttatgaggat gtcattatga aaaagtgcct cacactactg attgccaccg tcctgagcgg
    36301 aatctctctc acggcttatg ccgcgcaacc gatgagtaac ctggacagcg gtcagctacg
    36361 gcccgccggt accgtttcgg caactggcgc atcaaaccta agcgatctgg aggataaact
    36421 ggcagaaaaa gcgcgcgaac aaggcgcgaa aggttatgtc attaattccg caggcggaaa
    36481 tgaccagatg ttcggtactg caaccatcta caaataaccc caccaacgta actgcattcg
    36541 cctctggatg cagttttcgc ctgttttgta atcaaaaaac atcaaatatg aactcaatgt
    36601 aaataaatgt atttcttttt cgcgcaatgg gtgatagaaa atcgcgccaa gtgataatgc
    36661 ttatcaaaat tattatcact tgcatgaaca ctatcacggg attaacagtg gcatcgcatc
    36721 cgcagagatg ctttctcgtg gcagtgaaaa tttcaacata taagaaaaag tcacctgcaa
    36781 aatggaaaac aatcgcaatt tccctgccag acaatttcat tcgctcacgt tctttgccgg
    36841 tctttgtatt ggcatcacgc ctgtggctca ggcactcgcc gccgaagggc aaactaacgc
    36901 ggatgacacg ctggttgtcg aagcatcaac gccttcgctt tatgcgccac aacaatctgc
    36961 cgatccgaaa ttctcgcgtc cggtagcgga tactacccgc acgatgacgg tgatttctga
    37021 acaagtgatt aaagatcagg gcgcaaccaa ccttaccgac gcgctcaaaa acgcccccgg
    37081 cg
//
LOCUS       DAJTVJ010000030        36687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000030 DAJTVJ010000000
VERSION     DAJTVJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.030"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="OO448_001442"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="OO448_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO448_001443"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO448_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6336693.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO448_001444"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO448_001444"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6336694.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="OO448_001445"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="OO448_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /locus_tag="OO448_001446"
     CDS             complement(4104..4388)
                     /locus_tag="OO448_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336695.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO448_001447"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO448_001447"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6336696.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="OO448_001448"
     CDS             complement(5289..6497)
                     /locus_tag="OO448_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6336697.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="OO448_001449"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="OO448_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6336698.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="OO448_001450"
     CDS             complement(7292..8977)
                     /locus_tag="OO448_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6336699.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="OO448_001451"
     CDS             9247..9624
                     /locus_tag="OO448_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6336700.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO448_001452"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO448_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6336701.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="OO448_001453"
     CDS             10071..10358
                     /locus_tag="OO448_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6336702.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..11064
                     /gene="nfsA"
                     /locus_tag="OO448_001454"
     CDS             10342..11064
                     /gene="nfsA"
                     /locus_tag="OO448_001454"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6336703.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11125..12027
                     /gene="rimK"
                     /locus_tag="OO448_001455"
     CDS             11125..12027
                     /gene="rimK"
                     /locus_tag="OO448_001455"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6336704.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12115..12591
                     /locus_tag="OO448_001456"
     CDS             12115..12591
                     /locus_tag="OO448_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6336705.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12942..14054
                     /gene="potF"
                     /locus_tag="OO448_001457"
     CDS             12942..14054
                     /gene="potF"
                     /locus_tag="OO448_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6336706.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            14149..15282
                     /gene="potG"
                     /locus_tag="OO448_001458"
     CDS             14149..15282
                     /gene="potG"
                     /locus_tag="OO448_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6336707.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            15292..16245
                     /gene="potH"
                     /locus_tag="OO448_001459"
     CDS             15292..16245
                     /gene="potH"
                     /locus_tag="OO448_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6336708.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            16242..17087
                     /gene="potI"
                     /locus_tag="OO448_001460"
     CDS             16242..17087
                     /gene="potI"
                     /locus_tag="OO448_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6336709.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMVRAEKQRIRDIQ
                     RARHG"
     gene            17147..17635
                     /locus_tag="OO448_001461"
     CDS             17147..17635
                     /locus_tag="OO448_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6336710.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            17676..18803
                     /gene="rlmC"
                     /locus_tag="OO448_001462"
     CDS             17676..18803
                     /gene="rlmC"
                     /locus_tag="OO448_001462"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6336711.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OO448_001463"
     CDS             complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OO448_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6336712.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OO448_001464"
     CDS             complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OO448_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6336713.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OO448_001465"
     CDS             complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OO448_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6336714.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OO448_001466"
     CDS             complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OO448_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6336715.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHSEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OO448_001467"
     CDS             complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OO448_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6336716.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(23087..23602)
                     /locus_tag="OO448_001468"
     CDS             complement(23087..23602)
                     /locus_tag="OO448_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6336717.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            24251..26020
                     /locus_tag="OO448_001469"
     CDS             24251..26020
                     /locus_tag="OO448_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6336718.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            26231..26554
                     /locus_tag="OO448_001470"
     CDS             26231..26554
                     /locus_tag="OO448_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6336719.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            26551..27381
                     /gene="amiD"
                     /locus_tag="OO448_001471"
     CDS             26551..27381
                     /gene="amiD"
                     /locus_tag="OO448_001471"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6336720.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(27378..28391)
                     /locus_tag="OO448_001472"
     CDS             complement(27378..28391)
                     /locus_tag="OO448_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6336721.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(28490..29920)
                     /locus_tag="OO448_001473"
     CDS             complement(28490..29920)
                     /locus_tag="OO448_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6336722.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29931..30932)
                     /gene="ltaE"
                     /locus_tag="OO448_001474"
     CDS             complement(29931..30932)
                     /gene="ltaE"
                     /locus_tag="OO448_001474"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6336723.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30969..32687)
                     /gene="poxB"
                     /locus_tag="OO448_001475"
     CDS             complement(30969..32687)
                     /gene="poxB"
                     /locus_tag="OO448_001475"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6336724.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32820..33788)
                     /gene="hcr"
                     /locus_tag="OO448_001476"
     CDS             complement(32820..33788)
                     /gene="hcr"
                     /locus_tag="OO448_001476"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6336725.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(33800..35452)
                     /gene="hcp"
                     /locus_tag="OO448_001477"
                     /gene_synonym="priS"
     CDS             complement(33800..35452)
                     /gene="hcp"
                     /locus_tag="OO448_001477"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6336726.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(35596..36495)
                     /gene="lysO"
                     /locus_tag="OO448_001478"
     CDS             complement(35596..36495)
                     /gene="lysO"
                     /locus_tag="OO448_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6336727.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         9140 a         9051 c         9462 g         9034 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttgggcgggg tatatatcgg cggcctgcgc
    10741 aataatattg aagcggtgac gaaactgctg aaattaccgc agcatgttct gccgctgttt
    10801 gggctgtgcc ttggctggcc tgcggataat ccggatctca agccgcgttt accggcctcc
    10861 attttggtgc atgaaaacag ctatcaaccg ctggataaag acgcactggc gcagtatgac
    10921 gagcaactgg cggaatatta cctcacccgt ggcagcaata atcgccggga tacctggagc
    10981 gatcatatcc gccgaacaat cattaaagaa agccgcccat ttattctgga ttatttgcac
    11041 aaacagggtt gggcgacgcg ctaaaaccgc cacgtcgatg tatgatacgc gggcttttga
    11101 ccaggtctga cagagaggtg cagggtgaaa attgccatat tgtcccggga tggaacgctc
    11161 tattcgtgta agcggctgcg tgaagccgct atacagcgcg gtcacctggt tgaaattctt
    11221 gatccgcttt cttgctacat gaacataaat cctgcggcgt cttctattca ctacaaaggc
    11281 cgcaagttac cccattttga cgcagtgatc ccgcgtattg gcaccgccat taccttttat
    11341 gggacggcgg cactgcgcca gttcgagatg ctggggagct atccgctcaa tgagtcggtc
    11401 gccattgccc gggcgcgtga caaattgcgt tccatgcaac tgctggcgcg tcagggcatc
    11461 gacctgcctg tcacgggcat tgcgcattcg ccggatgata ccagcgattt aatcgacatg
    11521 gtcggtggtg cgccgctggt ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg
    11581 ctggcggaga cgcgtcaggc ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg
    11641 catattctgg tgcaggaata tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt
    11701 gttggcgatg aagtggtcgc tgcgattgaa cggcgggcga aagagggcga ttttcgttcc
    11761 aatttgcatc gtggcggcgc ggcaagcgtc gccagtatca caccacagga gcgtgaaatc
    11821 gcgataaaag ccgcgcgaac gatggcgctg gacgttgctg gtgtggatat tctgcgtgct
    11881 aatcgcgggc cgttggtgat ggaagtgaat gcgtcgccgg ggctggaagg aatagaaaaa
    11941 accactggta tcgacatcgc gggtaaaatg atccgctgga tcgaacgcca cgctacgaca
    12001 gaatattgcc tgaaaacggg tggttagtcg caatcacatt actgatcatg gttttgcctg
    12061 cgctttttgc gtaagctgtg ccggtctttt tatcgaaaga ggttgtacaa aattatgaca
    12121 tcgctggtcg ttcctggtct ggatacgctg cgtcaatggc tcgatgacct ggggatgagt
    12181 ttttttgaat gtgataactg tcaggctctg catctgcccc atatgcagaa tttcgacggt
    12241 gtctttgatg ccaaaatcga tctgatcgat aacacgatcc tgttttctgc catggcggaa
    12301 gtccgacctt cagccgtatt gccgctggcg gcggatttat ctgccatcaa tgccagttcg
    12361 ctgaccgtga aagcatttct tgatatgcag gatgataatc tgccaaagct ggtggtttgc
    12421 cagtctttat ccgttatgca gggcgtaacc tatgagcagt ttgcatggtt cgtgcgtcag
    12481 agcgaagagc agatttcgat ggtcattctt gaagctaatg cccatcaact gctgttaccg
    12541 actgacgatg aagggcaaaa caacgttacc gaaaactatt tcctccactg ataactcctt
    12601 tcgagcacgc agtcgctggt gcagtggctg cgcgctgcaa aattatctgc tgtttttaac
    12661 cttttcttaa agattatttc acttctcttg tgtcgatttg gctttatcac atagagcaaa
    12721 tacgcataaa aatttgttaa ataccgtttt ttaatccgag ctatagtctc aaaccctggc
    12781 taaagttatt cttgcgatgc ttttatatag cgagcagtgc tggccgggag agagttctct
    12841 tttcttacac cccgccgata aaaaatatgc acgtttattg catatctttt agtgtgacaa
    12901 cttttgttcg tttgttaacg aactttcaga aggaaagaga tatgaccgcc ttaaataaaa
    12961 aatggctttc gggtctggct gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg
    13021 ctgaacaaaa aacactccac atttataact ggtctgatta tatcgccccg gacacggtgg
    13081 ccaattttga aaaagaaacc ggtattaaag tcgtctacga tgttttcgac tctaacgaag
    13141 tactggaagg caaattaatg gcgggtagca ccggctttga cttggtggtt ccatctgcca
    13201 gctttcttga acgccagttg actgcgggag ttttccagcc gctggacaaa agcaaattgc
    13261 cggagtggaa gaatctcgac ccggagctgc tgaagctggt cgccaaacac gatcccgaca
    13321 ataaatttgc tatgccctat atgtgggcga cgaccgggat tggctataac gttgataaag
    13381 ttaaagctgt gctgggcgaa aacgcgcccg tcgatagctg ggacttgatc ctcaaacctg
    13441 aaaatctgga aaaactgaaa agctgcggtg tctctttcct ggatgcgcca gaagaagttt
    13501 ttgctaccgt gttgaattat ctcggcaaag atcccaacag cactaaagcg gatgattaca
    13561 ccggaccggc aaccgatctg ctgttaaagc tgcgcccgaa cattcgttat ttccattcat
    13621 ctcaatacat taacgacctg gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg
    13681 atgtctggca ggcgtcaaac cgcgcgaagg aagcgaagaa tggagtgaat gtctcgttct
    13741 cgattccaaa agaaggggcg atggcgttct ttgatgtatt cgccatgcct gcggatgcca
    13801 aaaacaaaga cgaagcctat cagttcctga attacctgct gcgcccggat gtagtagcgc
    13861 atatttccga ccatgtgttc tatgccaacg ccaataaagc agccacgccg ctggtgagtg
    13921 cggaagtccg tgagaacccg ggtatttatc ctccggcgga cgttcgtgcg aagctattca
    13981 ctctgaaagt acaggatccg aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga
    14041 agagcggaaa ataatccgca gccttagatg ccgggcgggc gcaccacacc agccggcaat
    14101 tcgcaccatc atggtgcgct tgcacacatt caatgccgga gagcagccgt gaatgacgct
    14161 atccctcgcc cgcaggcgaa aacccgtaag gcgctgacgc cgctattaga aatccgcaac
    14221 ctcaccaaat cctacgatgg tcagcatgcg gtcgatgatg tcagcctgac catctacaaa
    14281 ggtgaaatct tcgcgctgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg
    14341 ctggcaggtt ttgaacaacc ttctgccggg cagataatgc tcgatggcgt cgatttgtca
    14401 caggtgccgc cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg
    14461 catatgaccg tggaacagaa catcgctttt ggcctgaaac aggacaaact accgaaagcg
    14521 gaaattgcca gtcgggtcca tgagatgctc ggcctggtgc acatgcagga gttcgccaaa
    14581 cgcaaaccgc atcagctttc cggtggtcag cgacagcgtg tggccctggc ccgaagcctt
    14641 gccaaacgcc cgaaactatt actgctcgat gagccgatgg gcgcgctgga taaaaagctg
    14701 cgtgaccgga tgcagcttga agtggtggat attctggagc gcgtcggtgt gacttgtgtg
    14761 atggtcaccc acgatcagga agaggcgatg accatggcgg ggcgtatcgc cattatgaat
    14821 cgcgggaaat ttgtccagat tggcgagccg gaagagatct acgagcatcc aactacccgc
    14881 tacagcgctg aatttatcgg ttcggttaat gtctttgaag gtgtactcaa agagcgtcag
    14941 gaagatggcc tggtgcttga ttcgccgggg ctggtgcatc cactgaaagt cgatgcggat
    15001 gcttcagtgg tcgataacgt gccggtacac gtagcgctgc gcccggaaaa aatcatgctt
    15061 tgcgaagagc cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc
    15121 tatctcggcg atctttcggt gtatcacgtt cgactgaaaa gtgggcagat gatcagcgcc
    15181 cagctacaaa acgcccatcg tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt
    15241 ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgagtaca
    15301 cttgaacctg ctgcccagtc gaaaccgccg ggcggattta agctgtggtt gtcgcagctg
    15361 caaatgaagc atgggcgcaa actggtcatt gcgttgctat atatctggtt gatcttgctg
    15421 tttctgctgc catttctgat tgtctttaaa ataagcctgg gagagatggc gcgcgctatt
    15481 ccaccttata ccgaactgat ggagtgggct gacgggcaac tttccatcac tcttaatctc
    15541 ggtaattttc tgcaactgac cgacgatccg ctctattttg atgcttatct ccagtcgtta
    15601 caggtggcgg cgatttcgac attttgctgt ttactgatcg gctatccgct ggcgtgggcg
    15661 gtggcgcaca gtaagccttc gacccgtaat attttattac tactggtgat cctgccgtcg
    15721 tggacctcgt ttctgatccg cgtttatgcc tggatgggaa tattaaaaaa caacggtgtg
    15781 ctgaataatt ttctgctgtg gctgggggtt atcgatcaac cgctgaccat tctgcatacc
    15841 aatctggccg tttatatcgg cattgtttac gcttacgtgc cgtttatggt actgccgatt
    15901 tataccgcgt tgattcgtat tgattattcg ctggtggaag cagcgctgga tctcggtgca
    15961 cgaccgctga aaacgttctt tactgtgatc gtgccgctga ctaaaggtgg gattattgcc
    16021 ggatcgatgc tggtgtttat cccggctgtg ggcgagtttg tgatcccgga actgctcggt
    16081 ggcccggaca gcatcatgat cgggcgcgtg ttatggcaag agtttttcaa taaccgcgac
    16141 tggccggtgg cctcggcggt agcgatcatc atgttgctgc tgctaattgt gccgataatg
    16201 tggttccaca aacaccagca aaaaagcgtg ggagaacacg gatgaataat ttaccggtag
    16261 ttcgttcgcc ctggcggatt gtgattttgc tgctgggctt tacctttctc tacgcgccaa
    16321 tgctgatgct ggttatctat tcgtttaaca gctcgaagct ggtgacggtg tgggccggct
    16381 ggtcaacgcg ctggtatggt gagttattgc gcgatgacgc gatgatgagt gcggttggtt
    16441 taagcctgac aattgctgcc tgtgcggcaa cggcggcggc gatcctcggg actattgcgg
    16501 cggtggtgct ggtgcgtttt ggcaggtttc gcggatcaaa tggttttgcc tttatgatca
    16561 ccgcgccgct ggtgatgcca gatggcatca cgggcttgtc gctgttgtta ttattcgtcg
    16621 cgcttgctca tgccattggc tggcctgcgg accgcggtat gctcaccatc tggctggcgc
    16681 atgtcacttt ttgtacggct tatgtggcgg tcgttatttc ttcaagattg cgggaactgg
    16741 atcgctcgat agaagaagca gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg
    16801 tcattacgct accgatgatc atgcccgcga tcatttctgg ctggttactg gcttttactt
    16861 tgtcgcttga tgatctggtg atcgccagct ttgtttccgg gccgggagcc accacgttac
    16921 cgatgctggt cttttccagc gtgcggatgg gagtgaatcc ggaaatcaac gcccttgcaa
    16981 cgttaattct cggtgcggtc ggaattgtcg gatttatcgc ctggtatctg atggttcgcg
    17041 cagaaaaaca gcggatacgc gatatccagc gtgcaagaca tggctgaaga cactaaaatt
    17101 tgccaacctg gctacataat gccgcgcatg ccgcggcatt gttttcatgg aagacgaaac
    17161 gttgggattt tttaagaaaa catcttcatc tcatgctcgc ctgaatgtgc ctgcgctggt
    17221 gcaggtggcg gcgctcgcca ttattatgat ccgtggcctc gacgtgctga tgattttcaa
    17281 tacgctgggc gtgcgcggta ttggcgagtt cattcatcgc agcgtacaaa cctggagttt
    17341 aacgctggtc tttttaagca gtctggtgct ggttttcatt gagatctggt gtgcgttttc
    17401 actggtgaaa gggcgtcgct gggcgcgctg gctatatctg ctgacacaaa tcaccgccgc
    17461 aagttacttg tgggcggctt cgctggggta cggttatccg gagctgttca gcattcccgg
    17521 tgaatcaaaa cgtgaaatct tccatagcct gatgctgcag aagctgccgg atatgctcat
    17581 cctgatgctg ctgttcgttc cctcgaccag tcggcggttc ttccagttgc aataatgtgt
    17641 ataatcgtcg cccctgatga tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg
    17701 gtcgctgtcg ttcctgtcag tggataacgc agccgattcc agagcaactc tccgctaaaa
    17761 ccgccgatct taaaaatctg ctcgccgatt ttccggttga ggaatggtgc gcgccggtgt
    17821 caggcccgga acaagggttt cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa
    17881 aaccactgct cggtatgctg catcgcgatg gcacgccgga agacctttgt gactgcccgc
    17941 tttatcctgc ctcttttgcg cccgtttttg cggcgctaaa accgtttatc gcccgtgcgg
    18001 ggttaacgcc ctacaacgtg gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg
    18061 aaagccagag cgatggcggc atgatgctgc gctttgtact gcgttctgaa accaaactgg
    18121 cacaactgcg taaggcgctg ccgtggttac aggaacaact accgcaactg aaagttatta
    18181 ccgtcaatat tcagccggta catatggcga ttatggaagg ggagacggag atctacctga
    18241 ctgaacaaca ggcgctggca gagcgtttta atgacgtacc gctgtggatc cgtccgcaaa
    18301 gtttcttcca gactaatccg gcggtcgcca gccagctgta cgctaccgcg cgcgactggg
    18361 tacggcagct gccagttaaa catatgtggg atctgttctg cggtgtgggt ggctttggtt
    18421 tacactgcgc gacgcctgac atgcagttaa ccgggatcga aattgcatca gaggccattg
    18481 cctgtgcaaa gcagtcagcc gctgaactgg gcttaacgcg tttgcaattt caggcgctgg
    18541 actccactca gtttgccacc gctcaggggg atgtgccgga gctggtgctg gttaacccgc
    18601 cgcgccgcgg cattggtaaa ccgctgtgtg attatctctc aacgatggca ccgcgtttta
    18661 ttatctactc cagctgtaac gcccaaacca tggcgaaaga tatccgcgaa ctgccaggtt
    18721 accgcatcga acgggtacag ctttttgata tgttcccgca caccgcgcac tatgaagtga
    18781 tgacgctgct ggtgaagcaa taaaaaagcc gcaggtgcgg cttcagattg ctgatcaggt
    18841 gcgctttgtt catgccggat gcggcgtgta cgccttatcc ggcctacaaa atcatgctaa
    18901 atcaatacat tgcagagttg atgtgggcca gataagcgca gcgcatcagg caatcttact
    18961 tttgtcatca gtctcaatta ctgcgggaac cactggtcat tgattttctg atatgtaccg
    19021 tcagctttaa ttgctgccag cgcgttattc agtttttcca gcagagcttt gttatccgga
    19081 cgtacagcga tgcccagacc ggtgccgaag tattgcggat cggtcacttt ttcagtagca
    19141 acgcccagtt gcggattggt tttcagccat tcgtttacca ccgctgtgtc accaaatacc
    19201 ccatcaatac gaccattttt cagatcgata aaggcattct gataactgtc ataggagaca
    19261 gttttcactt cggggtgctg atcctgaata tatttctggt gcgtggtgcc gttttccatc
    19321 ccaatacgtt tgcctttcag atcggcaaac gttttgtagg tatctttttt ggcaatcacg
    19381 acggctgagt tttcatagta gggcgtggta aacgatacct gcttgctacg ctcgggcgtg
    19441 atatccatac cggagattac ggagtcatat tttctgaatt tcagtgacgg gatcaggctg
    19501 tcgaacgcgt gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc
    19561 agatcgatat caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag
    19621 gtggctgaaa cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca
    19681 agtaaagcgg caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt
    19741 tttggccgtg tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaat
    19801 atgattaaat aatttaaatg aaataaaaaa agacggataa cttagtgagt tgtccgtctt
    19861 cattataaga atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca
    19921 ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aaaccccccc
    19981 ggcgaatgta cgcagctaca ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc
    20041 agacgcatca tcagcgtcag caggccgttg acgaccaggt aaataatccc tgccgcaccg
    20101 aacaccatta catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc
    20161 agcgtaatgg tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa
    20221 gaagagagcg agcgtttaaa ggcatacggc agcaggagcg ccagcgtatc ttttttgctc
    20281 attcccaggg cgctacagga ctgccactga ccttctggga tcgcacgaat tgcaccgtaa
    20341 aacagctgcg tggtatacgc cgcactgttc agcgataacg caatcagcgc acataaccac
    20401 ggttctgaca acaaatgcca cagtgccgga tactcctgca acgtcggaaa ctggcccggc
    20461 ccgtaataaa tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa
    20521 ccccgcacca gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat
    20581 gccagaatca gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgtagccct
    20641 ttcatcagtt cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc
    20701 gcaggtcaat gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg
    20761 ccgccacaat gtaccaggta aatggttcct gggtacgagt agcgatgctt tttgtttgca
    20821 gcattaaatc attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc
    20881 actggttacc gaggccaggc agcgcatgac gccacatctg cggcatcacc agacggaaaa
    20941 agatagccga tttcgacagc cccagcgcct gaccagactc ccactgaccc accggcaccg
    21001 ctttcaacgc gccccgaagc gtttgcgagg cataggcggc atacagcagt gacagagcga
    21061 tgacaccaca aaggaacggg ctaacgtcga agttctcaat gtccatctgc actgggatct
    21121 gcacgaaccc aagattgata gtgaagccat ccgaaagcgt cagcagcagc tgcgaggagc
    21181 caaaatagat aaacagcacc accagaattt ctggcaggcc acgcagaatg gttaccagcg
    21241 ctgaacctgc ccacgcgaca ggacgccatt ttgccgactc ccataccgca aagaacatcg
    21301 ccagcgccag cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc
    21361 ttgctaaagg aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg
    21421 tttcgtaagt gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagct
    21481 cagtgttgcc ctgacgtacc gcgatgccga ggccagtgcc gaagtaatct ttatcggtca
    21541 ctttgtcgcc caccgccgcc agtttcgggt tatctttcag ccactcagtg accactgcgg
    21601 tgtcaccgaa gacgccgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc
    21661 tgtcatacgg aacggtagtg atttccgagt gcttatccat aatgaatttc tggtgtgtcg
    21721 tcccgttctg tacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt
    21781 gctgacccac aaacagggca gagttgtcat agtacggggt ggtaaacagc acctgctttt
    21841 cacgctccgg agtgatatcc atgcccgcca tcacggcttc tacgcgacgg aatttcaggc
    21901 ttgggatcag gctgtcaaac gcctggttag agaaagtgca ggttgcatca atctctttac
    21961 acagcgcttg tgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt
    22021 caaacggagg ataggatgct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa
    22081 gactaaaacc tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc
    22141 ttagtgagag agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc
    22201 gccttgttct acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc
    22261 cacttcaact tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat
    22321 gatgctgacg atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag
    22381 cagtacctgc ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc
    22441 agaaagatgc agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt
    22501 ttctgcacgg gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat
    22561 caggttttgc tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac
    22621 gttacgacgc aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt
    22681 gcctgcaatg ttgagcgtac cggagcgtgg catctcaagc agattgagta cacgcagcag
    22741 cgagctttta cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc
    22801 cagcgtgata tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg
    22861 aatactcatt gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc
    22921 tttgacagaa taattatgca atatttctgc tttaaaagtt aaaagcaaag cgcattattc
    22981 aataaacata gcacaaaata acgggggcgg tggtcggcga gcataaatgt cggcattcct
    23041 cacgaaatgc cggacaattt acggggttta ttggttgatc aaggcgttag cgattctcga
    23101 tggactgacg gagcgtaccc gccgtggcat gaacgctgcc gcctaagtag cgcacatcgt
    23161 cgatgaccca acactggcct tcctgaatca ttaacacttc atcctgccaa ccctggtcac
    23221 cctgtttgag atctacgcgc aatggaatgt tacgggcatc acgattaggg atagtcgatg
    23281 cactggcaac gtgggcgcta tctggcaagg tggttcgact ggagaacgga tcgctgctta
    23341 acaattcacg atgactatta tcgcggctgg catcgctaag aagggttgcc agtttgtcac
    23401 tcaaataggg gcgcagggcg gtaatgtcat tgctgcgatg ttgaatgcgg tagtcataga
    23461 attgttgagc cacgctgtca ggccccccct ggacacagga accggtgcgg ggaccgttat
    23521 ctttataagc tggcgtgacg gtggtacagg cactcagcag cagtgcacat ggaataagta
    23581 gagatagtga acggtagcgc ataatcattt ccttattagc ttgctgtctg cgtcacacta
    23641 cgaccaaacg ctatcagatt atttaataca ttgtttttaa tacaattctg aaacaatctg
    23701 aacacctgat tgggtttgag tccattgctg gctatccact caaggttgct cgtagctgac
    23761 ttacttaaat tacccaaaca atagacagtg taaaccattt gtgaggttga gcgtgttata
    23821 tggctgaaat gatcatttaa ggtaaatatg atagttctga aatggtggta taaatcctcc
    23881 tgtagaggtt aagagcatct gcaagaagcc agtttcacat ttataacgcg tgataaatga
    23941 acgcggttat tgtctaatca ttacaaaatt tttcacatgc gataagagat cctgcgatta
    24001 agatgattgc caaaattacg ttgtattttt gaaaatgagc gaaatttgat agtcccagct
    24061 ttgactctcg ataatattat ttatgctaca attgtggcta aagaatttaa gctaatgaat
    24121 tttagcgaag aataaattaa gttgtttcag tatctcgtta attaatatga atataaaagg
    24181 cgttgtgctt ttataattat aaagcacgag gagttcggtg gattatcgtt tttcttgggt
    24241 gatttttaat atgataaaca atatatctga tcaagcatca tcatttccag gcacacaact
    24301 aaatcaaagt gataatttcc tggattcact gagggaattt ttcgcgatat taaattccag
    24361 tcgtaagggt gagttgtcta catgggatac catctacttg catctgatat tagcaataaa
    24421 tgctgacagt gatctcataa aaaatgatgt gttgctcgcg gaaaatatgc catcagcgaa
    24481 ttaccaattt aatacttttt ttagtaatgc tttcgagact gatgttaaaa aatatctggg
    24541 taaatcagaa gataacgaag ttgaaataaa ggcgggtaat gaaagaatct ctattggaat
    24601 acgtaatgta tcgaatggga aacttgaaag acaacagttt cttttccctc tggactatga
    24661 gaataaatta caagatcaat tagacaagca ttttacgatt gaatcgcacc cattattata
    24721 caggcatacc attggtagta aaattgcgaa tgtgattttt gaaaaattat acagccgtat
    24781 tgactttaac aaagaacaat atatatcctt tataaagggc gcgtttattc atttttatga
    24841 ttatagcagg cgatatgcaa ttagtgagaa tatcgaaaaa gacgctgtaa caaacaatat
    24901 cgcattgatg aacacttttt atgacagtga taatacgtca ggtgaggttt taaataatga
    24961 ttttacagaa gaagagccat ttgaaactgc gttagatgta gagcatacaa tagtgcttgg
    25021 atttgctgat gataattttg aaacaaaacc tgttcattat caggatttat taacacgatt
    25081 tagtgccttt caagataccg tgtttaatct gtttcctgaa atgcgctctt cgcattacca
    25141 cgatatttgt agcgtatgtg tggatatgac aaagggaacg caatgcatga ttcacttgat
    25201 ggtgaatgaa gaagttttca tgtctctgcc tgttccagtt gccacgatgg tacgtgaaga
    25261 tgcaagtaat ttagtcaatc ttaaaacact actcaatgat ggttttttca ttaaatatag
    25321 tcattttaat gacgtggcat taataaagca aaacataagt aatctctact tatcacacac
    25381 tgttgtgaat gaaagcatat tgaaaaaatg ttgttttgaa aacggcagtt taggtgatgt
    25441 taaaataaca aatagcaata taataaataa tgtattcaaa aatgtttcgt ttagatctgt
    25501 taaaattaac aacgtaaata ctcattcttt aaaatttatt aattgccatt tttttaatgt
    25561 tgatatgata agagttaatt tcagcaaatg tctttttcat gactgtagta tgcatggtgt
    25621 caagataaaa ccatggttgc cagtaaaatg gacaaaagag ttaataaatg attatttgta
    25681 tggctgttta ctatctctgt actctatctg tgcaagggat atatataata tgagcgccgg
    25741 gaataacgtg aaggttgctg cggatgcttt ccttgaaatt attttctctc ttaagaataa
    25801 atattgcatt aaactattaa gcgctcagga acgggcgttt atttacgaat ttgcaagaat
    25861 gatcttcgct tatattaatg acaaaagcat tgagatctta ttactttctt gttttgcggc
    25921 gtctgaccaa aaagcaatcc agaggtatat acctcagtca caagatgggg aagactttag
    25981 gagtcattta caatataagt tgcctctttc cgctcattaa tgttgttggt gcaacataat
    26041 aagacaggta agcggtaccc tataatgaaa ccaatatcgg gttatgcaac ggataatcgt
    26101 aaagacttaa gtgggtgttt ttgacacaat gagatgattt cctaaactga tcaaaacctt
    26161 cttcccaaca tggcatatca gttcgataat gctttagaga tcgaaggctt aacaaacaag
    26221 gagatcgatc atgcaatttt ctacaacccc aactctggaa ggccagacca tcgttgaata
    26281 ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg atttctttgc
    26341 cggtatccgc gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac tgcgtaaagc
    26401 acgggagatc gcctttgagg aattaggctc ccaggcgcgg gcgctggggg ccgatgccgt
    26461 cgtcggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc tgatggttag
    26521 cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct ggtcgctgcc
    26581 gctctgttat tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga gggatatcag
    26641 cttgataccc gacgccaggc gcaggcggcg tatccgcgca ttaaagtgct ggtgatccac
    26701 tacaccgcag atgattttga tagctcgctg gcgacactga ccgataagca ggtcagctcg
    26761 cattatctgg tccctgcggt accaccgcga tacaacggta aaccgcgcat ctggcaactg
    26821 gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc
    26881 cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa atcggccgga
    26941 gtgaaatatt ttgccccgtt tgaaccgaca cagattcagg cgcttattcc actggcgaaa
    27001 gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc ggatatcgca
    27061 ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc gcagcagggg
    27121 attggtgcct ggccggatgc gcagcgggtt aacttttacc ttgccgggcg cgcgccgcac
    27181 acaccggtag ataccgcgtc attgctggag cttttggcgc gctacggtta tgacgttaaa
    27241 cctgatatga caccgcgcga gcagcggcgc gtgattatgg cattccagat gcatttccgc
    27301 ccgacgttat ataacggcga agcggatgca gaaactcagg cgattgccga agcattgctg
    27361 gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt
    27421 ttctcgatac cttcatctag ggtgatgatc ggctgataac ctaactcttc ctgcgcacgc
    27481 gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc cgtagtgggt cagcggcggc
    27541 tcttttgctg acttgcggcc taaacgctcc atgctgcggg cgatcatatc cagcatcggg
    27601 taggggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt ctgcacgatg
    27661 ctgcgcagtg tgcgatgctc gccgttggtg atattgtaca cacgcccgga aggtagctta
    27721 tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg tattttcata gtaggtcata
    27781 tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg catcatatgc
    27841 gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata
    27901 gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc gctggctgct
    27961 ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt aatatcgcga
    28021 tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt acgcacaccc
    28081 caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag atcgaaagcc
    28141 tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg
    28201 agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgccggaac aaactctgcg
    28261 cccatttttt ccagcaattt gcccattgcc tcgttgcgac cggtcgctcg cacgctgatg
    28321 cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc gccggtaacc
    28381 agtaccttca tatcaatcca ctgttgttga gaaaatgacg tgcgcattct tccgtgattt
    28441 cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat taatctgtgc
    28501 tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac agatgcgcgg
    28561 ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag aaagcgcgga
    28621 catcgatagt acgatagtcg cctttatctt ccaggctaaa acacagtcgt cccagccccg
    28681 acgctttcat gccaaataac aacgtaagtt gtttttccgg ttcaacgaca atcactttcc
    28741 agctatccac cgcatcgcca gtctgtaaat attcgcgctc cgggcggcct ttcgccagct
    28801 tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg ccaaagaaat
    28861 aacgctcttt accgccgatt tggttcacta cctgccataa agcagcaagg ctggcggacg
    28921 ttttaacggt aaaccccgcc tgtttggcaa aataaccgta ctccggtcgc cagcgggcaa
    28981 aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcctcc tctttcaacg
    29041 tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca cgtagcgcgg
    29101 tatcatccgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg
    29161 gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg agggggatgg
    29221 ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac
    29281 tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gctggccgga tgatctaaca
    29341 acgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc
    29401 agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgccg
    29461 ctgaacctgc gccaacgata attccggccc gaagttcggt cacaggtaca ttcgcttcac
    29521 gaagaatgtc cgccgtagcc tgacgagcac gcagatgatc cgactgctca tgtggcgggg
    29581 cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga
    29641 cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca
    29701 ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga
    29761 gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac
    29821 gtgccgccgc caggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa
    29881 tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc
    29941 aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc
    30001 gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg
    30061 cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc
    30121 atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct
    30181 tcctgcaacc gtgcgacgtt atttttcagg gcatacatcc cggcggcagc cagaatcccg
    30241 gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt aatgtaatca
    30301 cgattaccga cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg
    30361 aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta
    30421 aagatgcgcg caccgtcaac atgcagcgcc agattgcgct tgcgggtaaa ttcccatgct
    30481 tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttctag actgagtaat
    30541 ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt
    30601 agcgtgccgt cggcagccgc gtcgatgggt tgcggttgaa tactgcccag caccgctgcg
    30661 ccaccggctt caaacagata attatgcgcg gcctgaccga caatatactc ttcgccgcgt
    30721 tcgcagtgac tgagcagagc gaccaggtta gcctgggtgc cggtcggcag aaaaatggcg
    30781 gcttctttac cggaaagctc tgcggcgtag tcctgcagag cattaacggt agggtcgtct
    30841 ccgtaaacgt cgtccccaac cggggcggcc atcattgctt cgagcatggc gcggctcggt
    30901 cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc
    30961 cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg
    31021 ctgatgattg cgcgcaacat atacaagcta aatcctttgg cctgttcgag tttgatctgc
    31081 ggtggaatgg ctaactcttc tttggcgacc accacatcca ccagcaccgg accgtcgatg
    31141 gagaaggcgc gttgcagggc ttcatcaact tcagacgctt tttctacacg gatacccgta
    31201 atgccgcacg cttcggcaat gcgggcaaag tttgtgtcgt gtagttcggt gccgtcagtc
    31261 aaatagccac cagctttcat ctccatcgcc acaaagccca gcacgctgtt gttaaagacg
    31321 acaattttca ctggcagttt catctgcact actgagagga aatcgcccat caacatgcta
    31381 aaaccgccat cgccgcacat ggcgaccacc tgacgttctg gctctgtcgc ctgcgcaccc
    31441 agcgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc
    31501 ttgccgttca tttttagata acgtgccgcc cacaccgttg gcgtaccaac gtcacaggtg
    31561 aaaatagcgt catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg
    31621 gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcgtcgcg gtaatcttcc
    31681 agcgctttat ccagaaactt gcgatcggct ttttcttcca ccaatggaag caatgcacgc
    31741 agagtcgact tgatatcgcc gaccagtgcc atatccacct tgctgtgagc gccgatgctg
    31801 gctgggttga tatcaatctg aatgattttg gcatcggtcg ggtagaaggc gcggtaggga
    31861 aattgcgtgc cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag
    31921 aagccgatta acccggtcat tccaacatca tacggattat cgtattcgac atgttcttta
    31981 ccgcgcaggg catgaacaat aggcgcttta attttcccgg caaactcaac taactcttta
    32041 tgcgcccccg cgcagccgct gccacacatc agggcgatat tgctggaata acgcagcagt
    32101 tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggttgtgg cgcatgatac
    32161 cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcctgg taacacgaca
    32221 accgaaacgc cacggttaag caccgctttg cgcatggcaa tcgccagtac ttgtgggatc
    32281 tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg
    32341 tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc
    32401 agtaccggaa cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg
    32461 gggccgcacg atccggcgca gaccgccagt tctccgctaa gttgtgcttc agcgccagcg
    32521 gcaaaggccg ccacttcttc gtggcgggtg gacatccact cgatggtgcc catgcgatta
    32581 agactgtcac taagaccgtt cagagagtcg cctgtgactc cccagatgcg tttcacccct
    32641 gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc
    32701 ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagag
    32761 aaaggaggca ctaacggtta aatagcccga tgaaaggaat atcatcgggc ataaggcgat
    32821 tatgcgagaa ccaaatcccc ctgcggatgg caggagcagg ccagtacgta accttcagcg
    32881 atttcggcgt cggtcagcgt cattgtactg ctcaccgtat attcaccgga aaccactttt
    32941 gtcttacagc agccgcaaac acccgcacgg caggcagcga caaccggaac gttattgctt
    33001 tccagcgcct ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt
    33061 ttggtgaatt tcagaccgct ggtcgccgct tctgctactg gagtgaagaa tttctcttta
    33121 aagaaacgcg tcacgccgag cgctttcact tcctgctcta cccaatccat atacggagcc
    33181 gggccgcagg tcatcacggt acgtgaagct aagtcaggta cacctgccag cagttcgcga
    33241 gtgagacgac cagcgataaa gccttcggta acgttatttt ctgccaccag cgttaccgga
    33301 tagttacgcc actcatcggc gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc
    33361 cgcacatcgg cctgtggacg gttcttcgca agccagcgac gcatcgacat aatcggcgtg
    33421 acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat
    33481 tcccccatcg cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac
    33541 tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacgcct
    33601 ggcgtggagg aaatggtgta agcacgcagc gtttccgctg agtttcgcac gctgaccagt
    33661 gcatattgcc cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca
    33721 tccggcgttt cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg attcgttggc
    33781 atcgtcatcg acaaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg
    33841 tgatagaacg caggccgaat ttctcgttca gcaccgccag caggtctggt gtcaggaaac
    33901 caggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa
    33961 tgacgatcgc tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttcacac
    34021 cacagcccag tttctctgcc agagtgacag ccagaataat cgctgagtaa gcatcgttac
    34081 attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca agtttgttaa
    34141 agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg ctggtggcga
    34201 aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat
    34261 gacgcagttt ttcacggctc accagatcaa tcagcgtatc agcagcgcca agcagcgtct
    34321 ggcgaccaaa acccacggtg ataaggtgcg gaatttcgct gtacgggaag cctgccatct
    34381 gttgcgcctg ggtgataacc gcagagaaat catcaccgtc cagatgacgc acgccaggcc
    34441 agccaacaat gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga
    34501 tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactccact tgctgattct
    34561 gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat
    34621 gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca
    34681 gcaggttgta gagatctttg agatcgtgac cggaaatcag aatgcattta cccgccgtcg
    34741 ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca
    34801 gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt
    34861 tcatatcggc aggccaggtc cccagccacg ccatgatttt atggtactgg gcataaatat
    34921 cgttgtcgta ttgaccgaga acatgcgcgt gttccatata ggccgccgca cctttcaggc
    34981 catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt
    35041 taggggtaaa ttctgctgcc tgacgttgca gctcgccgag atcatcgctc accagttgca
    35101 ggtcagccat cgggttatcg acgcgcgcgt tggcatctac agccaggcat tgtgctttca
    35161 gcgcctcgcg caaggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt
    35221 taacgttggt cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca tcgtggttga
    35281 tgatgccgta ttcacacgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga
    35341 gtaaatcctg aaggtcagaa gtttccgccg ttttaccaca catcccctgc gcgtatgagc
    35401 agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac
    35461 cttttaaagt tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa
    35521 gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg gcagggatct
    35581 accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta acaggctaag
    35641 aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac
    35701 gggcagggtg aaatccattg atgtggcacc gcataagccc agtgcagtag agcggctgcg
    35761 gcgaatcagc ccagggatca acataatagc aatcagttca cgggccagat cattaaaaaa
    35821 cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata
    35881 ccagccgaaa ccggaggcca ttgccagcgc ggtattgatg gggagatcaa gaataaaggc
    35941 gttaattaaa ccaccaatta atgaactgac aaccaccacc acggcgacaa tcattccccg
    36001 gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa taccaacgag
    36061 gaaaagtagc aaaattaacg tgtattcact ggcttcggtc gcgtgttgta agaaagccag
    36121 tccactcaga ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactccag
    36181 cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcaggcc
    36241 tcgctccagc cacatcaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc
    36301 agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat
    36361 acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa taacttttaa
    36421 cgcagcttgt tggcgaagcg gaatgaggta acctacaatc aggggaacca gaatgattaa
    36481 cagcccagaa aacatgaaaa cccagtcctt gcaaagatga agtcgaaatg cgcgatgaca
    36541 cactactgaa agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcataagg
    36601 agcagtagag tattcgtttt catttaaaga tattcttgcg ctttaattac aaactgcacc
    36661 gatgttggtg gcgtcaaaat cgccgag
//
LOCUS       DAJTVJ010000031        36285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000031 DAJTVJ010000000
VERSION     DAJTVJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.031"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="OO448_001479"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="OO448_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6336728.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="OO448_001480"
     CDS             1867..1992
                     /locus_tag="OO448_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336729.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="OO448_001481"
     CDS             complement(1989..2060)
                     /locus_tag="OO448_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336730.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="OO448_001482"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="OO448_001482"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6336731.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO448_001483"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO448_001483"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6336732.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="OO448_001484"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="OO448_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6336733.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO448_001485"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO448_001485"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6336734.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO448_001486"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO448_001486"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6336735.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO448_001487"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO448_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6336736.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="OO448_001488"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="OO448_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6336737.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="OO448_001489"
     CDS             complement(9465..9872)
                     /locus_tag="OO448_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR6336738.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO448_001490"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO448_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6336739.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO448_001491"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO448_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6336740.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="OO448_001492"
     CDS             complement(11538..12197)
                     /locus_tag="OO448_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6336741.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="OO448_001493"
     CDS             complement(12361..12672)
                     /locus_tag="OO448_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6336742.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="OO448_001494"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="OO448_001494"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6336743.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="OO448_001495"
     CDS             complement(14207..14950)
                     /locus_tag="OO448_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6336744.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO448_001496"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO448_001496"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6336745.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO448_001497"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO448_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6336746.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO448_001498"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO448_001498"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6336747.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="OO448_001499"
     CDS             20005..20088
                     /locus_tag="OO448_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336748.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO448_001500"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO448_001500"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6336749.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO448_001501"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO448_001501"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6336750.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO448_001502"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO448_001502"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6336751.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO448_001503"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO448_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6336752.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="OO448_001504"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="OO448_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6336753.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="OO448_001505"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO448_001505"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="OO448_001506"
     CDS             25234..25836
                     /locus_tag="OO448_001506"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6336754.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="OO448_001507"
     CDS             complement(25937..25996)
                     /locus_tag="OO448_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6336755.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO448_001508"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO448_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6336756.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO448_001509"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO448_001509"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6336757.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO448_001510"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO448_001510"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6336758.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="OO448_001511"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="OO448_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6336759.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="OO448_001512"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="OO448_001512"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6336760.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="OO448_001513"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="OO448_001513"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6336761.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="OO448_001514"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="OO448_001514"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6336762.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="OO448_001515"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="OO448_001515"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6336763.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36285)
                     /locus_tag="OO448_001516"
     CDS             complement(35716..>36285)
                     /locus_tag="OO448_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6336764.1"
                     /translation="QLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSA
                     RVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDAN
                     MRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYM
                     QEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8685 a         9735 c         9164 g         8701 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactg
//
LOCUS       DAJTVJ010000032        35779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000032 DAJTVJ010000000
VERSION     DAJTVJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.032"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..581)
                     /locus_tag="OO448_001517"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OO448_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO448_001518"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO448_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6336765.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO448_001519"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO448_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6336766.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO448_001520"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO448_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6336767.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OO448_001521"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OO448_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6336768.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDCFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OO448_001522"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OO448_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6336769.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OO448_001523"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OO448_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6336770.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OO448_001524"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OO448_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6336771.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO448_001525"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO448_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6336772.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OO448_001526"
     CDS             complement(10994..11137)
                     /locus_tag="OO448_001526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336773.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OO448_001527"
     CDS             11138..11416
                     /locus_tag="OO448_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336774.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OO448_001528"
     CDS             complement(11463..13559)
                     /locus_tag="OO448_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6336775.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OO448_001529"
     CDS             complement(13559..14296)
                     /locus_tag="OO448_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6336776.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OO448_001530"
     CDS             complement(14293..14931)
                     /locus_tag="OO448_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6336777.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OO448_001531"
     CDS             complement(15045..15287)
                     /locus_tag="OO448_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336778.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO448_001532"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO448_001532"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6336779.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OO448_001533"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OO448_001533"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6336780.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OO448_001534"
     CDS             19524..19796
                     /locus_tag="OO448_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6336781.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO448_001535"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO448_001535"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6336782.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OO448_001536"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OO448_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OO448_001537"
     CDS             22056..22862
                     /locus_tag="OO448_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6336783.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OO448_001538"
     CDS             22872..23279
                     /locus_tag="OO448_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6336784.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OO448_001539"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OO448_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OO448_001540"
     CDS             23839..24636
                     /locus_tag="OO448_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6336785.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OO448_001541"
     CDS             24647..25471
                     /locus_tag="OO448_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6336786.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OO448_001542"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OO448_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="OO448_001543"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="OO448_001543"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6336787.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TCDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="OO448_001544"
     CDS             complement(27914..29545)
                     /locus_tag="OO448_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6336788.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO448_001545"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO448_001545"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6336789.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="OO448_001546"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="OO448_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6336790.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..35779
                     /gene="espL4"
                     /locus_tag="OO448_001547"
                     /pseudo
     CDS             34789..35779
                     /gene="espL4"
                     /locus_tag="OO448_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9230 c         9100 g         8851 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgactgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attattgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtctg tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccacca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa aacacgacac tcactcaaac cattgctcca atatttgaag
    35101 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
    35161 taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt caatgttatt
    35221 ttccggggcg accccaaaac cgtatagtat gccgacacat tgctgcacaa tatattaatg
    35281 atatttatca gaatgttgat tacaaacccc atcaagatga ttattcttca gctgaaaaat
    35341 ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt tccagccgtc
    35401 ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct agttatgaaa gcatattttg
    35461 acaagatgga atcaaatggc atcagtgtta tggcagccat attactggtg gataaccatg
    35521 ctttgacggt ccggctaaga ataaagaaca caactgaagg atgtacccat tacgtggttt
    35581 cggtttatga tcctaatgta actaacgaca aaataagaat catgagcgaa agcaaagagg
    35641 atattaaaca ctattctctg atggatttta tgaatgtaga ttatagcctc ctgaaatggt
    35701 caaatgatca tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc
    35761 tattgatatt aaaaggatc
//
LOCUS       DAJTVJ010000033        34981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000033 DAJTVJ010000000
VERSION     DAJTVJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.033"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..815)
                     /locus_tag="OO448_001548"
     CDS             complement(<1..815)
                     /locus_tag="OO448_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6336791.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO448_001549"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO448_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="OO448_001550"
     CDS             complement(1397..1873)
                     /locus_tag="OO448_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6336792.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO448_001551"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO448_001551"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6336793.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="OO448_001552"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="OO448_001552"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6336794.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="OO448_001553"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="OO448_001553"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6336795.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="OO448_001554"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="OO448_001554"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6336796.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="OO448_001555"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="OO448_001555"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6336797.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="OO448_001556"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="OO448_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6336798.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO448_001557"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO448_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6336799.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="OO448_001558"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="OO448_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6336800.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="OO448_001559"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="OO448_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6336801.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="OO448_001560"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="OO448_001560"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6336802.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="OO448_001561"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="OO448_001561"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6336803.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="OO448_001562"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="OO448_001562"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6336804.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="OO448_001563"
     CDS             13848..14552
                     /locus_tag="OO448_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6336805.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="OO448_001564"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="OO448_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15535..16246)
                     /locus_tag="OO448_001565"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="OO448_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO448_001566"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO448_001566"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6336806.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="OO448_001567"
     CDS             complement(17484..18245)
                     /locus_tag="OO448_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6336807.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="OO448_001568"
     CDS             18378..18788
                     /locus_tag="OO448_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6336808.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="OO448_001569"
     CDS             complement(18750..19856)
                     /locus_tag="OO448_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6336809.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="OO448_001570"
     CDS             complement(19867..21000)
                     /locus_tag="OO448_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6336810.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="OO448_001571"
     CDS             complement(20993..22729)
                     /locus_tag="OO448_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6336811.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO448_001572"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO448_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6336812.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO448_001573"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO448_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6336813.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="OO448_001574"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="OO448_001574"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR6336814.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO448_001575"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO448_001575"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6336815.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="OO448_001576"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="OO448_001576"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6336816.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="OO448_001577"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="OO448_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6336817.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="OO448_001578"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="OO448_001578"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6336818.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="OO448_001579"
     CDS             complement(31413..31673)
                     /locus_tag="OO448_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6336819.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO448_001580"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO448_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6336820.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO448_001581"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO448_001581"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6336821.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="OO448_001582"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="OO448_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8275 a         8975 c         9487 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 caggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcacacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAJTVJ010000034        34002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000034 DAJTVJ010000000
VERSION     DAJTVJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.034"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            13..515
                     /locus_tag="OO448_001583"
                     /pseudo
     CDS             13..515
                     /locus_tag="OO448_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(582..2039)
                     /gene="nuoN"
                     /locus_tag="OO448_001584"
     CDS             complement(582..2039)
                     /gene="nuoN"
                     /locus_tag="OO448_001584"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6336822.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2046..3575)
                     /gene="nuoM"
                     /locus_tag="OO448_001585"
     CDS             complement(2046..3575)
                     /gene="nuoM"
                     /locus_tag="OO448_001585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6336823.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3739..5580)
                     /gene="nuoL"
                     /locus_tag="OO448_001586"
     CDS             complement(3739..5580)
                     /gene="nuoL"
                     /locus_tag="OO448_001586"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6336824.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5577..5879)
                     /gene="nuoK"
                     /locus_tag="OO448_001587"
     CDS             complement(5577..5879)
                     /gene="nuoK"
                     /locus_tag="OO448_001587"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6336825.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5876..6430)
                     /gene="nuoJ"
                     /locus_tag="OO448_001588"
     CDS             complement(5876..6430)
                     /gene="nuoJ"
                     /locus_tag="OO448_001588"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6336826.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6442..6984)
                     /gene="nuoI"
                     /locus_tag="OO448_001589"
     CDS             complement(6442..6984)
                     /gene="nuoI"
                     /locus_tag="OO448_001589"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6336827.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6999..7976)
                     /gene="nuoH"
                     /locus_tag="OO448_001590"
     CDS             complement(6999..7976)
                     /gene="nuoH"
                     /locus_tag="OO448_001590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6336828.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7973..10699)
                     /gene="nuoG"
                     /locus_tag="OO448_001591"
     CDS             complement(7973..10699)
                     /gene="nuoG"
                     /locus_tag="OO448_001591"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6336829.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10752..12089)
                     /gene="nuoF"
                     /locus_tag="OO448_001592"
     CDS             complement(10752..12089)
                     /gene="nuoF"
                     /locus_tag="OO448_001592"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6336830.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12086..12586)
                     /gene="nuoE"
                     /locus_tag="OO448_001593"
     CDS             complement(12086..12586)
                     /gene="nuoE"
                     /locus_tag="OO448_001593"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6336831.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12589..14391)
                     /gene="nuoC"
                     /locus_tag="OO448_001594"
     CDS             complement(12589..14391)
                     /gene="nuoC"
                     /locus_tag="OO448_001594"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6336832.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14485..15147)
                     /gene="nuoB"
                     /locus_tag="OO448_001595"
     CDS             complement(14485..15147)
                     /gene="nuoB"
                     /locus_tag="OO448_001595"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6336833.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15163..15606)
                     /gene="nuoA"
                     /locus_tag="OO448_001596"
     CDS             complement(15163..15606)
                     /gene="nuoA"
                     /locus_tag="OO448_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6336834.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16235..17173)
                     /gene="lrhA"
                     /locus_tag="OO448_001597"
     CDS             complement(16235..17173)
                     /gene="lrhA"
                     /locus_tag="OO448_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6336835.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18088..19305
                     /gene="alaA"
                     /locus_tag="OO448_001598"
     CDS             18088..19305
                     /gene="alaA"
                     /locus_tag="OO448_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6336836.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19389..19988
                     /gene="yfbR"
                     /locus_tag="OO448_001599"
     CDS             19389..19988
                     /gene="yfbR"
                     /locus_tag="OO448_001599"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6336837.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20047..21879)
                     /locus_tag="OO448_001600"
     CDS             complement(20047..21879)
                     /locus_tag="OO448_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6336838.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21966..22614)
                     /gene="hxpA"
                     /locus_tag="OO448_001601"
                     /pseudo
     CDS             complement(21966..22614)
                     /gene="hxpA"
                     /locus_tag="OO448_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22625..23119)
                     /locus_tag="OO448_001602"
     CDS             complement(22625..23119)
                     /locus_tag="OO448_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6336839.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23202..23657)
                     /locus_tag="OO448_001603"
     CDS             complement(23202..23657)
                     /locus_tag="OO448_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6336840.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23995..25197
                     /gene="ackA"
                     /locus_tag="OO448_001604"
     CDS             23995..25197
                     /gene="ackA"
                     /locus_tag="OO448_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6336841.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25272..27416
                     /gene="pta"
                     /locus_tag="OO448_001605"
     CDS             25272..27416
                     /gene="pta"
                     /locus_tag="OO448_001605"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6336842.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27606..29126
                     /gene="yfcC"
                     /locus_tag="OO448_001606"
     CDS             27606..29126
                     /gene="yfcC"
                     /locus_tag="OO448_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6336843.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29159..29701)
                     /gene="yfcD"
                     /locus_tag="OO448_001607"
     CDS             complement(29159..29701)
                     /gene="yfcD"
                     /locus_tag="OO448_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6336844.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29759..30310)
                     /gene="yfcE"
                     /locus_tag="OO448_001608"
     CDS             complement(29759..30310)
                     /gene="yfcE"
                     /locus_tag="OO448_001608"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6336845.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30366..31010)
                     /gene="yfcF"
                     /locus_tag="OO448_001609"
     CDS             complement(30366..31010)
                     /gene="yfcF"
                     /locus_tag="OO448_001609"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6336846.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31146..31793
                     /gene="yfcG"
                     /locus_tag="OO448_001610"
     CDS             31146..31793
                     /gene="yfcG"
                     /locus_tag="OO448_001610"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6336847.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31850..32212
                     /gene="folX"
                     /locus_tag="OO448_001611"
     CDS             31850..32212
                     /gene="folX"
                     /locus_tag="OO448_001611"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6336848.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32233..33126
                     /locus_tag="OO448_001612"
     CDS             32233..33126
                     /locus_tag="OO448_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6336849.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33174..>34002)
                     /gene="rpnB"
                     /locus_tag="OO448_001613"
     CDS             complement(33174..>34002)
                     /gene="rpnB"
                     /locus_tag="OO448_001613"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6336850.1"
                     /translation="DTARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLL
                     WSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFY
                     HGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQK
                     HIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQE
                     KEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8480 a         9278 c         8565 g         7679 t
ORIGIN      
        1 gaggaaaaat aaatgggaat gattggttat tttgcagaaa ttgactcaga aaagattaac
       61 caactgcttg agtctactga aaaacctttg atggataata tacatgacac gctttcagga
      121 ttacgccgac tggatattga taaacgctgg gattttttac actttgggct gacaggcacc
      181 cctgcttttg atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttgg tgaacacagc
      241 ctcgaagatg gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca
      301 atcgaccgac tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga
      361 ctcaatgaaa tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc
      421 tttgcttcga tcatgctgat atggaaaagc tgattagcgc gtatcgacgt atgctgcgac
      481 aagggaatca tgcgctgacg gttattgttg ggtgatggtg tcagggcacc aaaaaaaacc
      541 gccgaacatg tcggcggctt tctgacttac aaactgatag cttacatcag cggcattgct
      601 aaccgcacaa tgctaatcag cggttgtggc catacaccca gcaccagtac caacagtgca
      661 gagatcagca ccacgatacc gcctgcgctg tactgccagt ttgacggtgc atcgcgaccc
      721 ggttgttccg gggcgtgcag gtacaggctc accgccacgc gcaggtagta gtaaaggccg
      781 cttgccgaac cgacaaccac ggcgcccacc agccaccaca agtgtgcctg gacaccgacc
      841 gccagcacgt agaacttacc aataaagccc agcgtcatcg gaataccggc cagagacagc
      901 atcatcaccg tcatcaccgc cgcgaggatc gggcgatgcc agaacagtcc gcggtaagag
      961 tacagggaat cagcatccgg gccacgatac gggctggaca tcaggctgac cacgccgaac
     1021 gcgccgaggc tgctaaacag ataaccggcc aggtaaaccc ctaccgcttc catcgacatc
     1081 tcgccagttt gcagcgcaat cagcgccacc agcagatagc cgaggtgaga gatagatgag
     1141 taaccgagca gacgtttgat attggtctgg ctcagcgcca tcaggttacc gaagatgatg
     1201 gaggcaaagg cgataatcgc cagcaccacg cgaatcgctt cgctgtcacc catcggtgcg
     1261 tagaggaaca gacgcatcac cacaccgaag atagcgattt tgctcgccgt cgccaagaaa
     1321 gtggaaaccg gcgcaggcgc gccctggtat acgtctggcg tccacaggtg gaacggcacc
     1381 agagagagtt tgaagccgag gccaacaatc atcaggccga aacctgccag caacagcggc
     1441 tcgttgagca taccgtcgcc aaggttttta cccaacgcga caaacgacag gtcgccagac
     1501 tgcgcataca ccagcgccat accaaacagc aggaaagaag acgctgcggc agaaaggatg
     1561 gtgtatttga tactggcttc cagtgaacgt ttctggcgga aagcgtaacc gaccaggcca
     1621 aacagcggca aagagatcag ttcgataccg aggaacagag acgccagatg gttggcattc
     1681 gccagcagga tcccgcccag cgcggcaatt aacaccaaca ggtagaactc gtccttgttg
     1741 tcgttatagc cttcaagcca cgggtaggcg aaagtacagg tggcgaggct cgccaacaat
     1801 accagcccgg tgtaaagcat ggcgaaacca tcaacgcgca tcagcggcgt aacgtccata
     1861 gcgcctgcct ggccgacaaa ccagagcgaa accagcgccg cgttaagccc aataaccgag
     1921 agcgtagcgt tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac aaccaccacc
     1981 gtcaagccga cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt tattgtcatg
     2041 gcgatttacg gccttgtagt agtaacggaa ttaacaaacc actgctggat attgccaatc
     2101 gcggagtgcg aggtatccag aatcggctgc ggatagaagc cctgcagtac cagcagcacc
     2161 accagcaaca ggatcataaa cagctcacgc agcgacatcc ctggcagttc ctggctggca
     2221 atctcgcttt tcgctttacc gaagtaagcg cgatgcaaca tcgccagcga ataaacagat
     2281 gcaaagacca gcccaaaggt agagataacg gtaatcaccg ggacaacctg gaagctgccg
     2341 aacagaatca taaattcgcc gacgaagtta ccggtgccag gcatcccaag cgttgccacc
     2401 gcaaagaaca gcgacagtgc tggcagccat ttcatcttgc tccacagacc gcccatcatg
     2461 cgcatgtcgc gggtatggat acgttcataa agctgaccac aaagaataaa cagacccgcc
     2521 gccgacaagc cgtgcgcaat catctggatt accgcgccct ggtaggccaa ctggctgccg
     2581 gtgtagatag caatcagcac gaagcccatg tgggaaaccg aggtgtaggc gatcagacgt
     2641 ttgatatcgg tctgggcgaa ggccatccac gcaccgtaga agatgccgat aacacccagc
     2701 cacatagcaa ttggcgcgaa ctctgccgac gcgttcggga acagcggcag ggagaaacgc
     2761 agcaaaccgt aagcggcagt tttcagcaag atccccgcga ggtcaacgga accggcggtc
     2821 ggagcctggg agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg cattttgact
     2881 gcgaaggcga tgaagaagcc cagcattaac aggtattccg caccactgga cattggcgta
     2941 ttcagcagct cttcatagtt gaaggtccag acgccggtcg cattgtagtg aacaaaaacc
     3001 agcgccagga tggcaatcaa catcaccaga ccactcgcct gggtgtaaat gaagaacttg
     3061 gttgccgccg tgatacgcgt tttaccgtca gaggctttat gcccccacag tgcgatcagg
     3121 aagtacatcg gcaccagcat catttcccag aagaagaaga acaggaacat gtcgatggca
     3181 aggaacacgc cgataacgcc gcccaggatc cacatcaggt tgaggtggaa gaagccctga
     3241 tatttttcga tctctttcca cgaacatagt accgccagca caccgagcag accggtcagc
     3301 acgaccatca gcagcgacag cccgtcaatg gcgagatgaa tagagatacc caaacgcggg
     3361 atccacggca tgtcgaattc agactgccac tgcggaattc cggcggattg cgtcagtgaa
     3421 taaccgccct gcaaccacag ttgcagcgac agcgccagcg tcaatcccat ggtgatcagc
     3481 gcgatccagc gtggcacctt gacgccaaag cgttcggtct gccagcacag gaagccgcca
     3541 ataaagggaa ttaatattag ccagggtagt aacatggcga tctttattcc ttgtaaaagt
     3601 cccgtcagga ccggattttc aacgaattct cacgacagaa aattctcttc ggactggggg
     3661 tagtgtcgga tgcaccgcga aggcggctta tccgacctac gttcttatat gcccaggggc
     3721 aatcccacaa tccttaactc aacgcagtac catcaacagt gccagcacca cgaccgcacc
     3781 gatgctcatg gatgccacat accagcgcag atagccgttc tcacttaaca gcagaccttt
     3841 acctgcaaag cgggaaagga cagccggaat gttcatcatt gagttcagcg gatcgcgttt
     3901 cagcaaccag gcaataccca ggaacggcct gacgaacact ttgtcgtaca gccagtcaaa
     3961 gccccaggcg ttgtaccacc aggtactcag caggcggccc ggcgcactgt tggcgatgga
     4021 ggtcaccaga gtacgtttac ccagccacag ccaagctgcc agcagaatgc cgacaatcgc
     4081 gaccacgcca gaggtaattt ccagggtcaa catgctgccg tgcgccagtt ccgtcgtttg
     4141 cggaagcacg ccctgcagcg gcggtacaat cagtgcgcca acgaaggtgg aaaggatcag
     4201 cagcacaatc agcggcaggc tgtgagttac ccctttcacg gcgtgagcgt gaatttgttc
     4261 ttttccgtgg aagacgatga aaatcatacg gaaggtgtag agcgaggtca taaacgcacc
     4321 gaccagacct gccaccatca gattgatatg accattcgcc atcgcacccg cgaggatctc
     4381 atccttactg aagaagcccg cagtgaccag cggcagtgcc gacagtgctg cgccgcccac
     4441 caggaagcag agataaacca gcggaataga tttacgcaga ccgcccatct tgaagatgtt
     4501 ctgttcgtga tggcaggcca gaatgacgga accggatgcc aggaacagca gcgctttaaa
     4561 gaacgcgtga gtcatcaagt ggaaaatcgc cgcatcccat gcctgcacgc caagcgcgag
     4621 gaacatgtag ccaatctggc tcatggtaga gtaagcgaga acacgtttga tgtcggtctg
     4681 taccagcgcg gcaaaaccgg ccagcagcag cgtaaccgcc ccgacaatgc ccaccagatg
     4741 cagaatttcc ggcgtcatca ggaacaggcc gtgggtacgg gcgatcaggt agacacccgc
     4801 ggttaccatg gttgcggcgt ggatcagcgc ggagacaggc gtcgggcccg ccatcgcgtc
     4861 ggcaagccat gtctgcaacg gcaactgcgc agatttaccg accgcaccgc ccagcagcat
     4921 cagcgtcgcc cacatcagca tgttattgcc gtcagcaaag tgcgctggtg ccagttccac
     4981 catttcgcgg aagttcaggg tgcccagttc gttgtaaaga atgaacagtg cgaaagcgag
     5041 gaaaacgtca cccacacggg tcacgacgaa cgctttcatt gccgctgcgc cattcttcgg
     5101 atcggtgtaa tagaaaccga tcagcagata ggagcacagg cccacgcctt cccagccgag
     5161 gtacatcagc agcaggttgt cggcaagcac cagaactacc atgctggcga tgaacaggtt
     5221 ggtgtaggcg aagaagcgag agtagccctc ttcaccgcgc atgtaccagg aggcgtacat
     5281 gtgaataagg aaacccacac cagtgaccac cgagagcatg gtcagcgaca ggccgtccag
     5341 caccaggtta aaaccgatgt taaagtcgcc taccgacatc cacgtccaca gcggctggct
     5401 gtatgcctgc tcgccgttag cgaagaaatc aacgccgata aaggcggtta ccagcgccgc
     5461 caggcccaca gagccaacgc cgacgatcgc cgaaacgttt tcagaccagc gcccacggga
     5521 gaacgccagc aggacgaagc caatcaatgg caaaataatg gttaaggcaa gcatgttcat
     5581 ccgcgcatct cacttactga atcgatgttc aggttctggc gacgacggtg aagttgcagc
     5641 agcagcgcaa ggccgatact cgcttctgcc gccgcgaggc tgatggcgag aatgtacatc
     5701 acctgaccgt cggtctgacc ccagtagctt ccggcgacaa caaaggccag cgcggaggcg
     5761 ttaatcatga tttccagacc aatcagcata aacagcagat tgcgacggat aaccagaccg
     5821 gttaagccaa gaatgaataa gattgccgcg aggatcagtc catgttgtaa ggggatcatg
     5881 cgtgctcctc cgtttttctt ttcgcgctgt cgtctttacg attgctcagc acttcacccg
     5941 cacgctcttc acgaccgacg tggaaggcca caaccagacc tgcgagcagc agcatagaag
     6001 ccagttccac cgccagtacg taaggcccga acagcgtaat acccactgct ttagcactga
     6061 ttggcgtacc gtcgataccc tgatcgttaa caccgaggat ggcgtaaaca atcaccacca
     6121 gcatgatggc cgacaaaatt gccggaccaa tccacacctg cggtttcagc cactggcgtt
     6181 cctgttcgat ttctgaaccg cccaggttga gcatcatcac cacgaacacg aacagcacca
     6241 taatggcacc cgcgtagacg ataatttcca gcgcaccggc gaagtaagcg cccagtgaga
     6301 agaacacccc ggaaatcgcc agcagcgaaa taatcaggta cagcagtgcg tgtaccggat
     6361 tggtatgggt gatcactcgc aaggtcgcaa gtatggctat caggccacag atataaaaag
     6421 cgaactccat tgcccctctc cttacggtaa caggctcttg acgtcgatag gcttggcttc
     6481 gttctctgct tcgcccttat ctttgccgtc gattgccata cctgccatcc ggtagaagtt
     6541 atattccggg tatttgcccg gaccggagat cagcagatcc tctttttcgt aaaccagatc
     6601 ctggcgcttg tattccccca tttcgaaatc cggggttaac tgaatcgccg tggtcggaca
     6661 ggcttcttcg cacagaccac agaaaatgca gcgtgagaag ttgatgcgga aaaattccgg
     6721 ataccagcga ccgtctttgg tttctgcttt ttgcagcgag atacagccga ccgggcaggc
     6781 taccgcgcag aggttacagg ctacgcaacg ctcttcgccg tccgggtcgc gggtcagaac
     6841 gatacgacca cgataacggg gcggcagata gatcggctct tccgggtaca ttcgcgtttc
     6901 gcgtttggcg aacgcgtgca ggccgatcat ccagatacta cgaacctggg tgccgaaacc
     6961 tactaacaat tcttttaagg tcatggtctt attgcccctt attgcgcctg ccagagaatg
     7021 acagccgccg ttaccagcaa gttgatcagc gtcagcggca ggcagatttt ccagccgaag
     7081 gacattacct ggtcataacg cggacgcggt aacgacgcac gaatcaaaat gaacatcatc
     7141 ataaagaacg cggttttcag cgcgaaccag atgaatggcg gtaacaacgg gccttgccag
     7201 ccaccgaaga acagcgtcac catcaatgca gagatggtca caatcccgat gtactcaccc
     7261 acgaagaaca gaccgaactt cataccggaa tattcaatgt ggtaaccatc cgccagttcc
     7321 tgctcggctt ccggctggtc aaacgggtga cggtgacata ccgccacgcc cgcgatggca
     7381 aaggtaataa aaccaaagaa ttgcgggata acgttccaca catgcgcctg gctgttgacg
     7441 atgtcggtca tgttgaatga accggcctgc gccaccacgc ccatcaggga aagcccgagg
     7501 aacacttcgt agctcagggt ctgcgcagaa gcacgcatcg cacccagcaa cgagtatttg
     7561 ttgttacttg accagcccgc aaacagcacc gcgtaaaccg ccagacctgc catcatcagg
     7621 aagaacaaaa tcccgatgtt caggtcggca accacccaac ccggactgac tggcacaatc
     7681 gcaaaggcca gcagcagcga ggtaaaggca atcatcggtg ccagggtaaa gatgacgcga
     7741 tccgagaatt tcgggatcca gtcttcttta aagaacattt tgatcatgtc cgcaaccagc
     7801 tggagcgaac cgccccagcc aacacggtta ggtccgtaac ggttctggaa cagacccagc
     7861 aggcgacgtt cgccaaagct catgaatgcc ccgcaggtga caaccaccag caggatcacc
     7921 accgctttga ggatggtcag caggatctca atcagttccg gtgatatcca actcattgtt
     7981 gtgcctcctt gagatcctca agatgcgcgc cagccagcac cggagcaata ccggacatac
     8041 ccatcggcaa gcccacctgc cctgccgtca gcccttcagc gatttcaacc ggcagcgtga
     8101 ccgtattgcc atcgtaacta aaggagacgt gtgtacctgc gttcacacct aacttcgcgg
     8161 catccgctgg gttgagtttg atgtacggct gcggcatacg gctctggaag accggagcac
     8221 gctgtgacaa ttcatcgctg ccaaacaggt ggtaatacgg cgcgatacgc catttcccgt
     8281 cctgcggctg gaagcgtgcc ggtacgctgg tgaagtaatc cagaccattt tcgctggttt
     8341 caaacagacg cacgcccgga tcgccaaagc gcagtttgcc gcccacttcg tcctggaatt
     8401 tgttccacgc ctgcggggag ttccagcccg gcgcccaggc aaacggcact tgcgaacggt
     8461 gcgcagtcgg ctggttgtta ccttccatcg agaaggtgaa catggtgtca atatcctgcg
     8521 gctgacgcgg ctcatgaacg ctgatattgg cgcgcatggc ggtacgaccg ctgtaacggt
     8581 gcggttcacg ggccagtttc tgcccacgaa tacggaatgt cgcatccggc gcagcatctt
     8641 tgatacctgc cagttccggg attttcgcca caacagcgtc aatcacatgg tcgagctgcg
     8701 tccagtccac ttcacggctc agcagggtgc tgtgcagcga gtgtaaccag cgccagcttt
     8761 ccagcatgac agttttgctg tcgtaataag caggatcgta aacctggaag aaacgttggg
     8821 cgcggccttc gttgttgatc accgtaccgt cgctttcagc aaaactggca gcagaaagta
     8881 ccagatgggc gttttccata atcgctgtgc gttgatgatc aaccaccatc accagcggcg
     8941 ctttagccag cgcagcattc acgcgggtag cggaagcgtg acgatgcaga tcgttttcca
     9001 gcaccaccac cgcgtcggcg cgtccggttt ccagttcggt caacgcttct tcaagcgaac
     9061 cgccacccat aatgcccagc cccatgctgt tgacggaacg ggcaatcatg gtgataccaa
     9121 cgtcagcgcc gcgacctttc agggctttcg cgacgttagc cgcagcctga atcacctcta
     9181 tgctaccggc gttcgtcccg gagataatca acggtttctt cgcacctgcc agtgcctgca
     9241 cgatgacgtc gattttgctt tgcagctcag gttcgatacc gtcaaccgct ggcgcagagt
     9301 tatccagcgc atgggcgatg gcaaaaccta aacgcgcctg atcttcaacc ggtgcgcggt
     9361 aagtccacgc cgcgatatca tccagacggg tgtcatcaac gttggtaaca aacagcggat
     9421 gcttcgcacg ttgaccgatg ttgaggattg ccgcaatctg ccagtcagcc actttctgtg
     9481 ctgccgccat ttcgcgcgct ttacctttca cagcctgacg cactgccagc gcgacgcgcg
     9541 cgccggtctg ggtaacgtct tcgcccagca ccagtaccgc atcgtaagat tcgatttcgc
     9601 gcagagccgg agtataaatg ccgccttcac gcagcacttt cagcgccagt tgcagacgtt
     9661 cctgctcacc gtgagcgata ccggtgtaga agttttcttc gcccaccagt tcacgcagcg
     9721 caaagttgct ttccacgctg gcacgcggag aaccaatacc gatcactttc ttcgactgac
     9781 gcagaatatc tgccgcgccc tgcattgcct gttcggcgtt gagggtaatg aaatcatcgc
     9841 cacgacgctg tactggctga cgcggacgat ccttcaggtt gacgtaaccg taaccgaaac
     9901 gaccacggtc gcagaggaag tagtggttta ccgtaccgtt gtaacggttt tcgatacgac
     9961 gcagttcgcc gtaacgttca ccggggctga tgttacagcc gatggaacat tgctggcaga
    10021 tgctcggcgc aaactgcata tcccatttac ggttgtaacg ctcggagtgc gttttgtcgg
    10081 taaatacgcc ggtcgggcaa atttcgacca ggttaccaga aaattcgctt tccagcgtgc
    10141 cgtcttccgg gcgaccgaag tagacgttgt cgtgcgcacc gtaaacgccc agatctgtac
    10201 cgtcagcgta atctttgtag taacgcacac agcggtagca ggcgatgcag cggttcattt
    10261 cgtgagagat gaatggcccc aaatcctgat tacggtgggt acgtttggtg aaacggtagc
    10321 gacggaagct gtgtccggtc atcacagtca tatcctgaag atggcagtta ccgccttctt
    10381 cacataccgg acagtcgtgc gggtggttgg tcattaacca ctcgaccaca ctttcacgga
    10441 actgtttcgc ttcttcgtcg tcaatggaaa taaaggtgcc atcggaagcc ggtgtcatac
    10501 aggacatcac caggcgacca cgcgtgtctt ccgcgttttg gtattgcttc accgcacact
    10561 ggcggcaagc accgacgctt cccagcgccg gatgccagca aaagtaagga atatcaaggc
    10621 ccagagacag acaagcttcc agcaggttgt ccgctccgtt gacctcgtat tctttgccgt
    10681 ctacatgaat tgtagccatt agcatgcttc cagttttctc ggtcagggac cgagcgttaa
    10741 tcgaaattcg gttaccagcg ctctttcagc aggttcggct gaatcccatt aatcaaatgg
    10801 gtattgctga acggctgttt gattcccgcc tcaaattctt cgcggaaata tttgatggcg
    10861 ctctgtaacg gctccactgc accaggtgcg tgggcacaga aagttttacc cgggcctaag
    10921 aatcgacaca gttgctcaag tgtttcgata tcgcccggct ggccttcgcc acgctccagc
    10981 gcacgcagaa ttttcacgct ccacggcaga ccgtcgcggc acggcgtaca ccagccgcag
    11041 gactcacggg cgaaaaactc ttccaggtta cgcaccagcg acaccatgtt gatctcatgg
    11101 tcaaccgcca tcgccagcgc cgtacccaga cggctgcccg ctttaccgat actttcgaat
    11161 tccatcggca gatcaaggtg cgcttcggtc aggaagtcag tccccgcgcc gcctggctgc
    11221 caagctttaa atttcagacc atcacgcata ccaccggcgt aatcttcgag gatctcgcgt
    11281 gcggtggtgc cgaacggcag ttcccacagt cccggatttt tcacccgacc ggagaagccc
    11341 atcagcttgg tgccagcatc tttacttttc gagatgttct gataccactc cacgccgtta
    11401 gcgaggatcg ccggaacgtt acacagggtt tcgacgttgt tgacacaggt cggtttgccc
    11461 catacgccgg aggttgccgg gaaggggggt ttcgagcgtg ggttagcacg acgcccttcc
    11521 agggagttga ttaacgctgt ttcttccccg cagatgtagc gccctgcccc ggtatggacg
    11581 aacagttcga agtcgaaacc tgttcccata atgtttttgc caagcaagcc cgcttcagtg
    11641 gcttcggcaa tggcgcggcg cagatttact gccgcttcga tatattcgcc acgcaggaag
    11701 atgtagccac ggtaagcttt cagcgcaaac gcggagatga gcataccttc caccagcagg
    11761 tgcggcagtt gctccatcaa caggcggtct ttataggtgc ccggctccat ttcatcggcg
    11821 ttacacagca ggtaacggat gttcatggat tcgtctttcg gcatcaggct ccacttcagg
    11881 ccagtcgaaa agcccgctcc gccgcgccct ttcagaccag cgtcttttac ctgattaacg
    11941 atttcgtccg gagacagccc ggtcagcgcc ttacgcgccc cttcgtaacc gtttttgctg
    12001 cggtattcgt ccagccacac tggctgtttg tcatcgcgca gacgccaggt cagcggatgc
    12061 gtttcgggag tacggataat gtttttcatt tataccgctc cagcagttca gggatcgctt
    12121 ccggggtcag atgcgcgtga gtgtcctcat cgatcatcat gtttggccct ttatcacagt
    12181 tccccaggca gcaagttggc agcagcgtaa agcggccatc aaatgtcgtt tgccctggtt
    12241 tgatgttcag ctttttctcg agcgccgcct gaataccctg atacccgttg atatgacaga
    12301 ccacgctgtc gcaataacgg atcacatggc gaccaaccgg ctggcggaag atctgactgt
    12361 agaacgttgc cacaccttcg acgtcgcttg ccggaatacc cagtacatcg gcgatcgcgt
    12421 ggatcgcacc atccggcacc cagccacgct gcttctgaac gattttcagc gcttcaatgg
    12481 acgccgcacg cgggtcttcg tagtggtgca tctcgtgctc aatcgcttca cgctctgccg
    12541 cactcagctc aaaagcctcg gtttgtggtt gttgattctc gtgcataatt agcggtccac
    12601 atctgacata acaaaatcga tactgcccag ataaacaatc aggtcagaca ccaggctgcc
    12661 gcggatagcc gccggaattt gctgcaaatg cgcataactc ggcgtgcgga tacgagtgcg
    12721 ataactcatg gtgctaccgt cgctggtcag gtagtaactg ttgatcccct tggtcgcctc
    12781 aatcatctgg aaagattcat tggcaggcat caccggcccc cacgacactt gcaggaagtg
    12841 ggtgatcagg gtttcgatat gttgcagcgt gcgctctttc ggcggcggcg tggtcagcgg
    12901 gtgatccgct ttgaacgggc cttccggcat gttgttgagg cactgctcaa gaatgcgcag
    12961 actctggcgc agctcttcca ctttcagcat tacgcgggtg tagcagtcag aaacaccacc
    13021 tccaaccggg atttcaaagt cgaagttttc atagccagaa taaggacgcg ccttacgcac
    13081 gtcgaagtcg atcccggtag cacgcaggcc cgcgccagtg gtgccccact ccagcgcctc
    13141 tttcgcgcca taggcggcaa cgccctggga acgacctttc agaatggtat tttgcagcgc
    13201 cgctttctcg taagacgcca gacgtttcgg catccagtcg aggaactcac gcagcaggcg
    13261 atcccagccg cgcggcaggt cgtgcgctac gccgccaata cggaaccacg ccgggtgcat
    13321 acggaaaccc gtgatcgctt ccaccagatc gtaaattttc tgacgatcgg taaaggcgaa
    13381 gaacacgggc gtcattgcgc cgacgtcctg aataaaggtc gagatgtaca gcaggtggct
    13441 gttgatacgg aacagttcgg agagcataac gcgaatgacg ttaacgcgat ccggcacggt
    13501 gatcccggcc agtttctcta ccgccagcac gtaaggcatt tcgttaacgc agccgccgag
    13561 gtattcaata cggtcggtat acggaatgta gctgtgccag gactggcgct cgcccatttt
    13621 ctccgcacca cggtggtggt agccgatgtc tggtacgcag tcgacaatct cttcgccgtc
    13681 gagttgtaaa acgatacgga aagccccgtg cgccgacggg tggttcggac cgaggttgag
    13741 gaacatgaag tcctcgtttt cggtgccgcg cttcatcccc cactcttccg gtttgaaggt
    13801 cagggcttcc atctccagat cctgtttggc tttggtcagc tcaaacggcg agaattcggt
    13861 agcgcgcgcc gggtaatctt tacgcagcgg gtgacctttc caggtttgcg gcatcatgat
    13921 gcggcgcagg ttcgggtgac cgtcgaaagt aatgccaaac agatcccagg tttcacgctc
    13981 ataccagtta gcgttcggga acagtttggt gaaggtcggt acgtgcaggt cgttttctgc
    14041 cagcgccacc ttcagcatga tgtcgcggtt acgatcgata gaaatcagat ggtagaaaac
    14101 ggaaaaatcc gcggcaggta acccttcgcg gtgtgtgcgc agacgttcgt ccatgccgtg
    14161 taagtcaaac agcatgacgt aaggtttcgg cagtttcttt aagaaatcgc caacttccag
    14221 taattgttca cgcttgatcc acacaacggg aaccccggtg cgagtcgcct gaacagtaaa
    14281 ggcatccggc ccaaaacggt tgcgcagttc gccaatcacc ggatcatcaa gatgatcacg
    14341 ggtctgccag gcgggttctt gcgcggttaa gtcggtcata ttgttcacca ttgcaaatgg
    14401 tccgtggtga ctgtcggcgc agtaagcttc gcgaaataga tttaggaata agcgaagaaa
    14461 aatccctttg ccgacaggcg caaattaaat ctcgtcaggt gtacgcaggt tggttacggc
    14521 aatgcgttca ccgcgcttgc gttcgcgctc tgattgcata ttggcgcgat aaacgccctg
    14581 atcgccaacc acccaggaga gcggacgacg ttctttgccg atagattcct gcaacagcat
    14641 cagtgcctgc atgtacgctt caggacgcgg cgggcagccc gggatataca catcaaccgg
    14701 gatgaattta tcgacgccct gcacaacgga ataaatatcg tacataccac cagagttggc
    14761 acaggcaccc attgagataa cccattttgg ttccagcatc tggtcataca gacgctgaat
    14821 aaccggtgcc attttggtaa agcaggttcc tgcaaccacc atcaggtcag cctgacgcgg
    14881 cgaagcacgc aatacttctg cgccaaaacg cgccacgtca tgcaccgcgg taaacgaagt
    14941 caccatctca acgtaacagc aggaaagacc gaagttatac ggccaaattg agtttttacg
    15001 accccagtta accatgtcat tgagcttgcc cataaacacg tttttgttaa cttcttgctc
    15061 cagagggtcg gttacgatct cctgcttttg cagggggtaa cggtcgttct caccgttggg
    15121 atctatgcgg gtgagcgtat aatccatctt aatgcctcgc ggttagcgtt gacgattagc
    15181 gatactgttc gtttccgggt tcatacgctc gcggcgtgaa cgcgcgggcg tccagtccag
    15241 cgcgccaata cgcaccagat aaaccagacc tgccagtaac acaaaaataa aaattgcagc
    15301 ttccacaaag cctacccagc cgctttcgcg gatagaggtt gaccatgcga acagatacag
    15361 cgcttcaacg tcgaagataa cgaagaacat ggccaccaga taaaacttgg cggacaggcg
    15421 taagcgggcg gagccgaccg agtcgatacc ggattcaaac ggcacgtttt tcgacctcgc
    15481 gcgtgcgcga ccgcctaaaa accaaccgcc taccagcatc aggcaacaca ggccaatggc
    15541 aacgataaga aagatagcga atgcccagtg atgagcgatg acttcagtgg atgttgacat
    15601 actcattgct tactcatcaa aagtagcgcc agattcactg ctcttcacgg cagatggacg
    15661 ccacatcgat tcatggggag gaataaaaaa aaccttacaa tcactgtaga aattctttta
    15721 tacagctaat tgatgtggtc ttttactcct ttctataacc ttttgtcaac tttaacaaaa
    15781 gtttcttcac attagtttac ataatatcaa caccattagc atttaatgcc ctttcacgcc
    15841 agatccttga cgactccagg ataattagat gttgttgaat cgtgtccgtt gtgaagcaat
    15901 ggaaaaaata cgggtctatt ttgacaggaa tttgtgtcga ttcctccccc caaaagagag
    15961 tattttcttg atctgtgaca cgcttttgtc attccataac aaaaacgcag caacaaattt
    16021 acgcattttt aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgttcgg
    16081 gacgtgaatc tctggtgatt gaaaaatgaa cagttttgta cgttctgcac tatgcgagga
    16141 aggattttta ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttac
    16201 actattgtct caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca
    16261 atgaatcatc cccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct
    16321 ccatcgcctg atagatcacc tgggccagct cattattact ggacgggtca tagcacagca
    16381 aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtacg
    16441 ggctcatcat ttcaatcggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa
    16501 cggccggaag cgtcgaggcg acataagcca gtcgccatgg aatatctgct ttattaagcg
    16561 tcgccagcac catatcgcga aacggacttg gatcatccag caatacaaga gggatcggct
    16621 cgcctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt
    16681 tcaatgcctt aaacgctgaa ggacgatggg tggttaccat caaatccact tcctgggatt
    16741 ccagcatttc tgccatatag gcattacgtt taacgcggac atccagcgct aatttcggat
    16801 aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag
    16861 caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat
    16921 taaaacgcag tattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt
    16981 tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt
    17041 gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca
    17101 gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat
    17161 ttgcacttat catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc
    17221 acacacaaac aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg
    17281 gacgaaagta aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata
    17341 atgcctgaag tagatcacag aatatatctt cagggatcgc atatctatta agttactcac
    17401 tcttttctat ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac
    17461 tctcgtgcag catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaggcggcat
    17521 caagagcagg gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag
    17581 ggtcattgcg aattatctga tgcaaagtta tgttaggcaa agtaatcttc tattattaat
    17641 aagcacatca aaaccttttt gaatattaaa taataattaa ttagcatcat cctcattcat
    17701 taattccgct taacaatagt ttcacacctt gcccccattg cgacacctcg gaaatcatca
    17761 cgctgttaaa ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctta
    17821 aatgaggcat tttcacacaa ttatcttaca gataaaaaaa ccagacttgc aattaagaat
    17881 cagaacaatt cactatataa cattgcatgc aaagcatata cgcctcatta ttttgtcatt
    17941 attaagttat taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc
    18001 ttcaaaacac cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg
    18061 cgataacagc aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag
    18121 aatgtctgtt atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa
    18181 ggtaacaagg tactgaaact gaacatcggc aacccagccc cattcggttt tgacgcgcca
    18241 gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc
    18301 aaagggcttt actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt
    18361 gatgttaccg tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca
    18421 atgcaggcat tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccactc
    18481 tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc
    18541 tctgactggt tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg
    18601 atcgttatta tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg
    18661 gagattgtgg agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac
    18721 aaaattctct acgacgatgc tgagcatcac tcaattgcgc cactggcacc tgacctgctg
    18781 accattacct ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg
    18841 atggtgttga acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg
    18901 gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt
    18961 ggttatcaga gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac
    19021 cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg
    19081 ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg
    19141 gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg
    19201 ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct
    19261 ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc
    19321 cggagattgc atccggcagc gttatcccgc cacaatgacc tgatgatgtc atcatacgta
    19381 aggtcactat gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc
    19441 gctggccgct aatgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag
    19501 cgatggtcgc ccatgcgctg gcagctatca aaaatcgaaa atttggcggt aatgtcaacg
    19561 ccgaacgtat cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc
    19621 tccctactcc ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa
    19681 aaatcgctca gcaaaaactg gtcaatatgg ttccggaaga gctgcgggat atctttgcgc
    19741 cgttaattga cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg
    19801 cactgtgtgc atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc
    19861 tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact
    19921 tcatggaagt atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac
    19981 cgctgtaagc agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg
    20041 ctcttgtcaa aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat
    20101 ggtgaacggt acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac
    20161 cagtgtgtta acaggtgaag aaaccggtgt cataaaggcg gcggatgctg ccatcgccac
    20221 gaccatcgcg aatggataag gcgacacccc catcgttttg gcagcagcca gcgcaatcgg
    20281 agccatcaac accgccgtcg cggtattaga gataaatagc ccaataaccg ccgacaagac
    20341 aaacaaacac cccagcatca tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt
    20401 caccgccagc gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac
    20461 gatcaaaata atgctcggcc agtgaatgga tttataggcg ctttcagcat ctatacagcg
    20521 gaatttcccc atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatctgt
    20581 cagcattaac gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc
    20641 gggcgatgct tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt
    20701 ggccagcata ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag
    20761 aggctcatcc gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt
    20821 cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc
    20881 aggaattaat gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc
    20941 gcgcagtacc atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc
    21001 cgcagacata tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt
    21061 cacgataacg cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg
    21121 ctcacgtaat ttgagatcat ccagccgttg accaatcatt ggcgatccgg ggcgaatagc
    21181 cagacgtcgc gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg
    21241 cgtccagcct tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa
    21301 catatacaaa atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact
    21361 gaagccgtga tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc
    21421 caccagcgtc atcatgccgc ttatcagccc ggcaaaactc aacggcatca tcagacgtga
    21481 cggcgaagtt tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac
    21541 gccggttgaa ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat
    21601 tttgatttca ctgttgcccg caactttgac cagccatgtt cccattacgg tggcaacacc
    21661 ggtacggacc aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc
    21721 agaaaagccg gaaaatactt cggggactgt cagcgttccg cttaatgcaa acgcgacaat
    21781 aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga caatggcaac
    21841 ggccagtaat gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag
    21901 ggagatgtct tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag
    21961 gtgtttcact gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga
    22021 ctgtggagga ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca
    22081 atgacatgac aacccgccgc caggccagaa agcacgccag cggagcatct tccaccacca
    22141 cacactcctg cggcgcaagc cccagcagct gcgcgcctaa cagatacgca tcaggttctg
    22201 gttttccgcg cttcactcgc tcagcggtta caaacacctc tggtgcggga agcccagcta
    22261 ttttatggcg cgctcgcgct accggcatgg agccagaagt cacaatggcc cacggaatac
    22321 ctgctttatt caaatgactg agtaaggcga ttgcccccgg aagcgggtaa taccttcggt
    22381 ttccgtggcc tcgatgtgct ccagacgcgt aaactcggcg gcaatatcag cctcggattt
    22441 gcccgccata aaatggcgca gagaggtgat cgcctgttta ccgtgaatga aagccagcac
    22501 ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg
    22561 cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc gcacgcgggc
    22621 ctcctcaggc attgataatt tgattaattt cgttggcgct caaatggtac tgacgcgggc
    22681 aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc tgggcgttaa
    22741 aaccgtgagt tccggcgtca aaatgggtat agcgcccttc cacattaacc ataaagcgga
    22801 cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt
    22861 catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca tgatacatct
    22921 ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac tcgcgatcca
    22981 attcacgcat ctgtaatccg taacctcgct caataattgt ttgcaggcga cggtaacgtt
    23041 cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa ataagacgtt
    23101 gcgcgttggt catttccatt gttgactcct gtatcactct actacggtga aaaaaaagaa
    23161 ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc atcaaggaaa
    23221 gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg gtaatcaggc
    23281 ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg aacttcataa
    23341 aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag ccaccacaat
    23401 ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc aagctgaccg
    23461 cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa acggatcgca
    23521 taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg tttttccagt
    23581 ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt aacagaacga
    23641 ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa aatttgtgca
    23701 aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc actatcaggt
    23761 atcctttagc agcctgaagg cctaagtagt acatattcat tgagtcgtca aattcatata
    23821 cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat ttcttccatc
    23881 atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta
    23941 cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac ttccatgtcg
    24001 agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc catcatcgat
    24061 gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct gcctgaagca
    24121 cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc aggcgccgct
    24181 cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc agaactgtct
    24241 gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta taccagctcc
    24301 gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt tgcaccgctg
    24361 cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc acagctgaaa
    24421 gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga agagtcttac
    24481 ctctacgccc tgccttacaa cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac
    24541 ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa
    24601 gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc tatccgcaac
    24661 ggtaaatgcg ttgacacctc tatgggcctg accccgctgg aaggtctggt catgggtacc
    24721 cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct gggcatgagc
    24781 gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg
    24841 accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc gaagcgcgca
    24901 atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc gctgatggat
    24961 ggtcgtctgg acgctgttgt attcactggt ggtatcggtg aaaatgccgc gatggttcgt
    25021 gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga acgcaacctg
    25081 gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt
    25141 atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac tgcctgattt
    25201 cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa
    25261 gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc gtcggtctga
    25321 ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt
    25381 tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact acgactatcg
    25441 tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc tacgttgaag
    25501 gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca aactaccacg
    25561 ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc
    25621 agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg
    25681 ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa ctgacccgca
    25741 acagcttcgg cggtgccaaa aacaccaaca tcaccggcgt tatcgttaac aaactgaacg
    25801 caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc gacgactctt
    25861 ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc cctctgccgg
    25921 ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc gatatggctc
    25981 gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc gttaaatccg
    26041 tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc ggttctctgc
    26101 tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca gccatgaacg
    26161 gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg cgcatttcta
    26221 aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac accaacacct
    26281 ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac gatcacgaac
    26341 gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg atcgaatctc
    26401 tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc tgcgttccgt tatcagctga
    26461 ctgaacttgc gcgcaaagcg ggcaaacgta tcgtactgcc ggaaggtgac gaaccgcgta
    26521 ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta
    26581 atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactgggt gcagggattg
    26641 aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc gaactgcgta
    26701 agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac gtggtgctcg
    26761 gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct gttcacacta
    26821 ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg
    26881 tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt gactgtgcga
    26941 tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc gctgattccg
    27001 ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc ggtacttctg
    27061 gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc
    27121 ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct gacgttgcga
    27181 aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgtttatc ttcccggatc
    27241 tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg atctccatcg
    27301 ggccgatgct gcagggtatg cgcaagccgg ttaacgacct gtcccgtggc gcactggttg
    27361 acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag cagtaacctc
    27421 gtcatcatcc gcagctttgc gctgcggata tctgaaccgg aaataatcac tatttccggt
    27481 tttttattct cttaatctgc attaatcctt tctgattatc ttgcttaact gcgctgcatc
    27541 aatgaattgc gccatttcac tttacatact taccactttg ttttgtgcaa gggaatattt
    27601 gcgctatgtc cgcaatcact gaatccaaac caacaagaag atgggcaatg cccgatacgt
    27661 tggtgattat cttttttgtt gctattttaa ccagccttgc cacctgggta gttccggtgg
    27721 ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag
    27781 tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct gagtatcacc
    27841 gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag
    27901 gattaacctc aggatcgaaa tacgggacag ctgttggcat catcatgttt atgctggtga
    27961 ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt atcctggcgc
    28021 ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt attctgtttt
    28081 cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc attatcatcg
    28141 caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg acctatattg
    28201 ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtc gttgctcagg
    28261 ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg tgggttatcg
    28321 ccactatgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc
    28381 ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg gatgttgaac
    28441 aacgtccgtt tacctttggt gactggctgg tattgattgt cctgaccgcc gtaatggtct
    28501 gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc agccagttct
    28561 tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac ggcatgacgg
    28621 ttaataccat ggcttcatcc tttaccgaag ggacgcgaat gatgatcgcc cctgccctgc
    28681 tggtgggttt cgccaaaggg attttgctgc tggtcggtaa tggtgaagcg ggtgatgcca
    28741 gcgtgttaaa taccatcctc aacagcattg ccaatgccat tagcggtctg gacaacgcgg
    28801 tcgcggcctg gtttatgttg ctcttccagg cggtatttaa tttcttcgtg acgtccggtt
    28861 ctggtcaggc ggcgttaacc atgccgttac tggcaccgct tggcgatctg gtcggtgtta
    28921 accgtcaggt taccgtgctg gcattccagt ttggtgatgg cttcagtcac atcatttacc
    28981 caacctcagc ttcgttaatg gcaactctcg gtgtgtgcag ggtggacttc cgtaactggc
    29041 tgaaggtggg cgcgacccta cttggactgc tgtttattat gtccagcgtc gtagtgatcg
    29101 gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg ctcttttttc
    29161 attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc cacaacgcca
    29221 gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca
    29281 gccagcaaac ttcactgact tcatcttcct gtagggcgaa gggaccgtga gagacgcagc
    29341 tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg
    29401 caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg gattccagca
    29461 gttgctcatc ggcctgaacc acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt
    29521 cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc acgacgatgt
    29581 aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct tgtgcaatga
    29641 cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga cgctgttcca
    29701 ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg tgggtaaatt
    29761 acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt acactaagta
    29821 cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata ctcaccgagc
    29881 cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg gtatgaccgt
    29941 acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa agatgaccat
    30001 gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg gttatcggga
    30061 aatgcagcag catttgatcc acttcgctgt cgcagttgcc gcgcacagcg ataaccttat
    30121 gtgccacttc attaagccgt tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat
    30181 tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca ccgctttggg
    30241 caaacaactc cagaacacgt tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca
    30301 tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt
    30361 tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga cgctcgctgc
    30421 cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg tagtaccagg
    30481 cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa taaattcggc
    30541 tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact ggcttttccc
    30601 tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg acgctcttcg
    30661 cggatgggca tcagatcgct gcgcagccag gcctgaatcc gtcgtgcacg cgcacgattt
    30721 tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc ttccagatac
    30781 tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa cggcacacgg
    30841 cgtgtctgac cgtaaccttg ccacgtcggc ttcaaatgtt caccgctgtc gaggtcgatg
    30901 gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga taacacataa
    30961 ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg
    31021 gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca cgtgacgaat
    31081 actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa acaaccggag
    31141 ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt acgctgtttc
    31201 tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa ggcggtcagt
    31261 ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt gttgatcatt
    31321 ctcctgccga tggcggcgaa ccgctaagcc tctttgagtc tggtgccatt ttgttgtatc
    31381 tgactgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc gccacattac
    31441 agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat catcatttta
    31501 atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt gaaactcagc
    31561 gtctttacca tgtactaaac aagcggctgg aaaactcgcc ctggctggga ggcgagaact
    31621 acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc cagcgaattg
    31681 acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg cgccctgcca
    31741 ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt taacagaaac
    31801 aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa tggcacaacc
    31861 tgccgctatt attcgtataa agaaccttcg tttacgtacg tttatcggaa ttaaggaaga
    31921 agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc ccgccgataa
    31981 agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat
    32041 tattcagcat gtagagaata accgtttctc tttgttggaa aaattaactc aggatgtgct
    32101 cgatatcgca cgtgaacatc actgggtgac gtatgctgaa gtggagatcg ataaactgca
    32161 cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt aaccgccata
    32221 tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg tcgccatttg
    32281 attccgcgtt tgctggagct gggccatcaa atcacggtag tgacgcgtaa cccacagaaa
    32341 gccagttccg ttctcggccc tcgggtgacg ctatggcaag ggctagccga tcaaagcaac
    32401 ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga taaacgctgg
    32461 actcacgagc aaaaagagcg tctctgccaa agccgctgga atatcacgca aaaactggtc
    32521 gatttgatta atgccagcga cacgccgccg tcggtattca tttccggctc ggcaacgggc
    32581 tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca taacgaattt
    32641 actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag tgacaaaacg
    32701 cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat tctcggtaaa
    32761 atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg gcagtatctg
    32821 gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga taacgacctg
    32881 cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt tgcccatgcg
    32941 ctcggtcaag cgctgcatcg cccggccatt ttgcgcgtcc ccgcaaccgc cattcggctg
    33001 ttaatgggcg aatcttcaat actggtatta ggtggacaac gcgcgctgcc taaacggctg
    33061 gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc ggatgtcgtt
    33121 cgctgatgtg gtttacagca aacatccgcc agttaactcc cggtgttaca ggattagtgg
    33181 ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa ctctctgtct
    33241 aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt gccctgcatt
    33301 gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg
    33361 cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt ttgtaatacg
    33421 taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca
    33481 atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat taactccaac
    33541 agcgccattt tgcggtgttg cataatctcg tcatccggca ccacggtaat atccaccaac
    33601 ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc atcaagccag
    33661 cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg gatcaccaat
    33721 ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga ataacgcatc
    33781 atgcgaaaag ccattaattc ttccagctta ctttggtgct ctatcactac ataaatataa
    33841 cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg ccgcaggtct
    33901 tcatcaataa aactgtttga ttctagttta agcgtcgtta aatcacacag tttgcgcagc
    33961 ggcgcgggaa gatgaatatc aataaaatcc cgcgcggtgt ct
//
LOCUS       DAJTVJ010000035        33803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000035 DAJTVJ010000000
VERSION     DAJTVJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.035"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..2409)
                     /gene="acrF"
                     /locus_tag="OO448_001614"
                     /pseudo
     CDS             complement(1..2409)
                     /gene="acrF"
                     /locus_tag="OO448_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2421..3578)
                     /gene="acrE"
                     /locus_tag="OO448_001615"
     CDS             complement(2421..3578)
                     /gene="acrE"
                     /locus_tag="OO448_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR6336851.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            3977..4639
                     /gene="acrS"
                     /locus_tag="OO448_001616"
     CDS             3977..4639
                     /gene="acrS"
                     /locus_tag="OO448_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR6336852.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4642..4821)
                     /locus_tag="OO448_001617"
     CDS             complement(4642..4821)
                     /locus_tag="OO448_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6336853.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(4905..5789)
                     /gene="yhdJ"
                     /locus_tag="OO448_001618"
     CDS             complement(4905..5789)
                     /gene="yhdJ"
                     /locus_tag="OO448_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6336854.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(5875..6171)
                     /gene="fis"
                     /locus_tag="OO448_001619"
     CDS             complement(5875..6171)
                     /gene="fis"
                     /locus_tag="OO448_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6336855.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6197..7162)
                     /gene="dusB"
                     /locus_tag="OO448_001620"
     CDS             complement(6197..7162)
                     /gene="dusB"
                     /locus_tag="OO448_001620"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6336856.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7491..8372)
                     /gene="prmA"
                     /locus_tag="OO448_001621"
     CDS             complement(7491..8372)
                     /gene="prmA"
                     /locus_tag="OO448_001621"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6336857.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8384..9835)
                     /gene="panF"
                     /locus_tag="OO448_001622"
     CDS             complement(8384..9835)
                     /gene="panF"
                     /locus_tag="OO448_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6336858.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(9825..10067)
                     /locus_tag="OO448_001623"
     CDS             complement(9825..10067)
                     /locus_tag="OO448_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6336859.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10176..11525)
                     /gene="accC"
                     /locus_tag="OO448_001624"
     CDS             complement(10176..11525)
                     /gene="accC"
                     /locus_tag="OO448_001624"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6336860.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11536..12006)
                     /gene="accB"
                     /locus_tag="OO448_001625"
                     /gene_synonym="fabE"
     CDS             complement(11536..12006)
                     /gene="accB"
                     /locus_tag="OO448_001625"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6336861.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(12984..13958)
                     /locus_tag="OO448_001626"
     CDS             complement(12984..13958)
                     /locus_tag="OO448_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6336862.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14110..16050
                     /gene="csrD"
                     /locus_tag="OO448_001627"
     CDS             14110..16050
                     /gene="csrD"
                     /locus_tag="OO448_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6336863.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16355..17398
                     /gene="mreB"
                     /locus_tag="OO448_001628"
     CDS             16355..17398
                     /gene="mreB"
                     /locus_tag="OO448_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6336864.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17464..18567
                     /gene="mreC"
                     /locus_tag="OO448_001629"
     CDS             17464..18567
                     /gene="mreC"
                     /locus_tag="OO448_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6336865.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18567..19055
                     /gene="mreD"
                     /locus_tag="OO448_001630"
     CDS             18567..19055
                     /gene="mreD"
                     /locus_tag="OO448_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6336866.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19064..19657
                     /gene="yhdE"
                     /locus_tag="OO448_001631"
     CDS             19064..19657
                     /gene="yhdE"
                     /locus_tag="OO448_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6336867.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19647..21116
                     /gene="rng"
                     /locus_tag="OO448_001632"
     CDS             19647..21116
                     /gene="rng"
                     /locus_tag="OO448_001632"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6336868.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21184..24984
                     /gene="yhdP"
                     /locus_tag="OO448_001633"
     CDS             21184..24984
                     /gene="yhdP"
                     /locus_tag="OO448_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6336869.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25140..26585
                     /gene="tldD"
                     /locus_tag="OO448_001634"
     CDS             25140..26585
                     /gene="tldD"
                     /locus_tag="OO448_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6336870.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26713..27578)
                     /locus_tag="OO448_001635"
                     /pseudo
     CDS             complement(26713..27578)
                     /locus_tag="OO448_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27761..27964
                     /locus_tag="OO448_001636"
     CDS             27761..27964
                     /locus_tag="OO448_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6336871.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            27972..28904
                     /gene="aaeA"
                     /locus_tag="OO448_001637"
     CDS             27972..28904
                     /gene="aaeA"
                     /locus_tag="OO448_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6336872.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            28910..30877
                     /gene="aaeB"
                     /locus_tag="OO448_001638"
     CDS             28910..30877
                     /gene="aaeB"
                     /locus_tag="OO448_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6336873.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            30969..31241
                     /locus_tag="OO448_001639"
     CDS             30969..31241
                     /locus_tag="OO448_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6336874.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31297..31560)
                     /gene="yhcN"
                     /locus_tag="OO448_001640"
     CDS             complement(31297..31560)
                     /gene="yhcN"
                     /locus_tag="OO448_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6336875.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(31925..32395)
                     /gene="argR"
                     /locus_tag="OO448_001641"
     CDS             complement(31925..32395)
                     /gene="argR"
                     /locus_tag="OO448_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6336876.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            32830..33768
                     /gene="mdh"
                     /locus_tag="OO448_001642"
     CDS             32830..33768
                     /gene="mdh"
                     /locus_tag="OO448_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6336877.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8209 a         8397 c         8853 g         8344 t
ORIGIN      
        1 gagaatggca ccatttcgcc gttggcgctg cggacataaa gtttatcgac atcttctggc
       61 agcatacgga atttggcatc cgcctgaaca tacacctttt tcacgcgacc acggtcgatg
      121 aagtcgttaa cgtaagtccc acccagcgcc gttgaaatgg tctgattgat gtcagaaagt
      181 gaaacaccta atgcctgcgc cttttcctgg tcaacttcca gtttaaactg cgcggtgtct
      241 tccagaccat taggacgcac gctgactaag ctggcaggat gttgcgccgc catgccaagc
      301 aactggttac gggcctgggt cagggcatcg tgacccagcc cagcctgatc aattaactca
      361 aagtcgaaac ccgttgccgt gcccagttca acaatggctg gcatattgaa tggaatgaca
      421 aaaccgtcgc ggatcttgcc caattccatt ttggcacgat ggattaccgc ttccgcactg
      481 ttttcgtcac cactacgctc ttcccacggt ttcagactga cgaaagccat accggcgttt
      541 tgtgcctggc cgctgaagct aaagccgtta accgtaaaga cactttctac gttcgctttc
      601 tcgttattca gatagtaatc cgtcacttga tccaacactt tttgcgtccg ctcttgcgtc
      661 gcgccagcgg gtaactgaat catggtcaga aagacaccct gatcctcttc aggtaagaag
      721 gaagaaggaa gacggaagac gtaaaaacaa caccaccatt cctgcaacaa tcagcgcata
      781 gatcagtaaa tatcgtcctg tggaaccgag gattttgccg acgctgttgg tgtagtggtt
      841 aacgctatga tcgaaggtgg tattaaacca gccgaagaaa ccgcccttat tttcgtgatg
      901 ctcagcagag acgggtttaa gcagcgttgc acataacgca ggggtaagaa tcaatgccac
      961 cagaacagaa agcgccattg ccgaaacgat ggtgatagag aactggcgat aaattgcccc
     1021 agtagaaccg ccgaagaatg ccatcggaat aaataccgct gacagcacca tcgcgatacc
     1081 caccagtgcg ccctgaattt gcgacatcga tttttccgtc gcttcttttg gcgggagctt
     1141 atcctccatc atcacgcgct cgacgttctc caccaccact atcgcatcat cgacgagcag
     1201 cccgatggca agcaccatcc cgaacatcgt tagtgtgttg atggagtaac caaaagcggc
     1261 gaggatggca aacgtcccta acaacaccac gggtaccgca atggtgggga tcagcgttgc
     1321 tcgcatattc tgcaagaaca gatacatcac caggaacacc agcataatgg cttcgaacag
     1381 cgtttttacc acttcgtgaa tagaaagctg gacgaatggc gtggtgtcat aagggtagag
     1441 aaccttcatt ccctgcggga agaatggctg taattccgcc agttttgcct taatggcttt
     1501 cgcggtattg agagcgttcg cgccggttgc tagcttaatc cccaggcccg ccgccggttt
     1561 tccgttgata cgagcgataa cgttatagtt ttcaccgcca agttcaactc gtgcgacatc
     1621 tttcaggcgt accaccgagc cgtcactgtt tacgcgcagg gttactttgc cgaattcttc
     1681 cggatttttt aaccgcgtct gagcaataat cgaggcgttc aactgttgcc ctggtaacgc
     1741 tggcgttccg cccaactgcc cggcagcgat ctgatcgttc tgtaccttca actggttaat
     1801 cacatcaacc ggtgtcagtt tatatttgtt tagcagatcg gcgtccagcc agatacgcat
     1861 cgcatactgt gcgccgaaaa gctgtacgtc accgacgcca ttcagacggc taagcgtatc
     1921 tttaacgtta gaggccacat agtccgagat atcgtcctgt gtggtgtctg ggttatcaga
     1981 gacaaagccc gccaccatca aatagctgct actggacttt tcaacactga tcccctgctg
     2041 ctgaacctcc tgcggcagca acggcgtggc gagctggagt ttgttctgca cctgcacttg
     2101 cgcgatatca ggatcggtcc cggactggaa ggtaagggta attgtcacgc taccggcgga
     2161 atcgctggtg gaggacatat acatcaggtt atcgatacca ttcatattct gttcgataac
     2221 ctgcgtcacc gtatcctgca cggtctgcgc atcagcacct ggatagtttg cctaaacaga
     2281 aaccgcaggc ggtgcaatgg ttggatactg agcgacgggc aattgtagga tcgccagtgc
     2341 gcccgccatc atcagaataa tggccagcac ccatgcaaat atcggtcgtc gaataaaaaa
     2401 gtttgccatg tcagattacc ttacttcgat gcagtatctg cgggggtatc ggtagtggct
     2461 ttaacctgct ctcccggacg cgctttttgc aggccgctga caatgacttg atcgccagat
     2521 ttcagacctt cgctaatcaa ccatttatcg ccaatcgcct gactggcaac gacagggcgc
     2581 gcttccacct gacttttatc gttaacaatc agcacggttg catcaccacg cggtgtgcgg
     2641 ctaacgcctt gttgcgggat aagaatggcg tcaggttgga cgccttcatc aatccgtgca
     2701 cgcacaaaca tacccggcaa aagcgtatgt tgcgggttag ggaagacagc acgtagggtt
     2761 atggagccgg tgcattcatc aacggtcaca tcagagaatt gcagcgtacc tttcagggga
     2821 taggtttgac cgttttccat gaccaactct acgttgctgg tggcgttttc cttatgcaaa
     2881 tttccttgct ctacggattg cttcagcctc ataaaatcgt tgctggattg ggtcacatca
     2941 acgtagatag gatcgagctg ctggacagtc gccagttcag tcgtttgccc attagtgaca
     3001 agagcgcctt cggtcacggt cgatttgccg atacgtccgc taattggcgc agtcactttg
     3061 gtataagcga gattgatgcg agcgctttcg actgtggctt ttgcggcaat cacggcggca
     3121 tcggcctggc gagcatcagc aatggcctgg tcgtactcct gctggctgat gtatttcgta
     3181 cccacgagcg gaacgtaacg ttttaccgtc aaatgcgcga tggcggcggc ggcttcactt
     3241 ttcgccagtt cgcctttcgc gctgtcataa tttgcctgat aggtcgcggg atcgatctgg
     3301 tacagggact ggcctgcttg tacatcgctg ccttcagtga aattacgatt cagtacgatc
     3361 ccgctaacct gtgggcgaac ttcggctata cggtaagcat tggtgcggcc tggtaattca
     3421 gtcttaactt ctaacggggc cgttttcaca atatgaacgg taacctgcgg ttcaccgacg
     3481 tgagctttct cttctccctt atcgttacaa ccgacgatta aagccgcgga gatcagaata
     3541 aaggagggca ggaggaaaaa cctggcatgt ttcgtcatta ctattcctca aaaaaacaaa
     3601 agcgcgttat ttacccagaa aggcaacgcg ttaactcgca gaaagaaaaa tacagtttgc
     3661 tatcctacaa attatcattc gtcgatgtaa ggaatagtta tgaatactgg cgtctcaagg
     3721 cacataaaca caaaaaaaga ttaatattct actgttttat tttgacgcgg gttgaaagag
     3781 gcagaattaa aacctcgtaa attgaaatat atattcatgt ggcgaatgta ttttaggtaa
     3841 ataatatata ttatttattt gacgatttat agcaactcaa tgttaccctc tcgtataaat
     3901 acacattaga tgatagatta accttcgcta ttttctcacc ctgtgtcgaa tatatttatt
     3961 tcctgaataa ttaatcatgg caaaaagaac caaagccgaa gctctgaaga cccggcaaga
     4021 actgattgaa actgccatcg cccagtttgc gcaacatggc gtaagcaaga cgacgttcaa
     4081 cgacattgcc gatgccgcta acgttacgcg tggcgctatc tactggcact tcgagaacaa
     4141 gacccaactg tttaatgaga tgtggttgca acagccttca ttgcgggagt taatccagga
     4201 acacttgacg gctggattag agcatgaccc gtttcaacaa ttgcgtgaaa aattgattgt
     4261 cggcttgcaa tatattgcca aaattccccg ccagcaggcg ttgctgaaaa tcttatatca
     4321 caaatgtgaa tttaatgatg ggatgctggc cgagggagtg atacgcgaaa agatgggctt
     4381 taatccgcag actctccgcg aagtattgca ggcgtgtcag caacaaggtt gtgtagcaaa
     4441 taacctcgat ttagatgttg tgatgattat tattgatggt gccttcagcg gaattgttca
     4501 aaactggtta atgaatatgg cgggttatga tctttataaa caagcacccg cgctggtcga
     4561 taacgtatta agaatgttca tgccagatga aaacataacg aaattaattc atcaaacaaa
     4621 tgaattaagt gtcatgtaaa ttcacattct gtcctgaaaa ttcagttcgt cacagttaac
     4681 cagttttagt ggataaactg agttcatatt acggcatttg tctgtttctg ttgtcagcag
     4741 ttcaatccac tgcatcagta aagtatcaaa cagaaaaacg aagacagcga aaacgacgag
     4801 ccaccaatac ttgcgaatca ttacctgagc ctgtgcatgt ttaaccaagt ctggcaggaa
     4861 atatacacga aatgaataat ctgaaaacca ggcttacact tcctttactt tgcaatgaga
     4921 tcggggtcaa cttcgcttaa ccggcttcgt tttgacaggt tgccggtctt tctttttttc
     4981 actttcgcca gttcttccgc ggagtaatgc gacgcgacat ccagccgtcg aagccccatt
     5041 ttgatgtact cgctgttgat ctcaatacca atgaattttc gtccgctggc gatggctacg
     5101 gcaccggtag taaagctacc ggcaaacggg tcgagaacga tatcgcctgg gttggaagag
     5161 gcgagaataa tgcgtttcag taaggcttcc ggtttttgcg tcgggtggtt ctcatattca
     5221 tccatcaaat aacgcacgcg cggaaaatcc cagacgttac ccggtacttt ttgatgattg
     5281 tatggctgtg gaggattttt gcgataatcg atcaacgcgc gctgcgatcc ggttttggct
     5341 tctaccagaa tagcatcacc gttgaatgtg tagttctttg cgtctttcac catcatcagg
     5401 atgggttcgt acatggagcc gtagtgtttt ttcgcctgta ctccagaact gtcatatgac
     5461 cagacgatgc gacttttgat ggtaaaaagc ttgcggcact ggagatcgat aaagggcatg
     5521 ttttccgtac tgttcataat gtacatgctg ccctgctttt tcagaacgcg gtggcactct
     5581 acaatcactt caaacagcca gtcgataaac agatcttctt tccaggcttc gatcagacca
     5641 tcaaaatttt taccgatgtt atacggtggg tcggcaaaga tcagatcgac gctttcagta
     5701 ggtagctttt taagttcggc aagggcatca ccgtgaataa tggtcttagc ttcattacca
     5761 aaccgggtcg gttcacatcc tgttctcatg gtcactccct ttgtgacacc tataaaaaag
     5821 gcgcttcccc atgccgagta gcgccttttt aatcaagcat ttagctaacc tgaattagtt
     5881 catgccgtat tttttcaatt ttttacgcag cgtaccacgg ttgatgccca tcatcagcgc
     5941 agcacgggtc tggttaccac gggtgtattg catcaccatg tccaacaggg gctgttctac
     6001 ttcagccagt accagctcat agaggtcatt cacatcctga ccattcagtt gagcaaaata
     6061 gttcttcagt gcctgtttaa ccgagtcacg caggggtttt tgggttacct gatcctgaga
     6121 gttaacggta gaaacggtca gtacgtcaga atttacgcgt tgttcgaaca tagttctgtc
     6181 agctctttat ttctgtttac gcaaaatttt cgaagtatgc ctccaacgcc tccagctgtt
     6241 cgctggcatc ctcaatggcg ttgaatgtgc gccgaaactg gtcatttgga gcgtgttcct
     6301 ggagatacca ggaaacgtgt ttacgtgcaa ttcggtaccc ttttgccgga ccataaaagt
     6361 catgcagttc ccgaacgtgc gcgcaaagca agcgcttaac ctctgccaaa ggcagcgggg
     6421 gcagcaactc cccagtgtcc agataatgct ggatttcccg aaagatccag ggtcttccct
     6481 gagctgcgcg gcctatcatc agggcatccg cccctgtata gtcgagcaca gctctggctt
     6541 taagcgggtc agtaatgtcg ccattcgcga taaccggaat ggaaactttc tgcttaactg
     6601 cccgaatact gtcgtactca gcttctccat tgaacaaaca ggcgcgtgta cggccatgaa
     6661 tggtcagagc ctgaatgcca cagtcttcag ccagttgggc aatctcttcg cagttacggt
     6721 gttccggtgc ccagccggtg cgaatcttca gggtaacagg aacgtccact gcattgacga
     6781 cctcggtaag gatcgattta acgacatccg ggtactgcaa gagggctgaa cctgcgagct
     6841 tgcgattcac ttttttagcc gggcaaccca tattgatatc aataatctgg gcaccgcttt
     6901 ccacgttaat acgtgctgca tctgccattt ctttcggatc gctaccagca atttgcacgg
     6961 tgcgaatacc gggttcatca atgtgcacca tccgtaaacg agatttgtcg ctttcccaaa
     7021 cctgtgggtt agaagacatc atctcggata ctgtcaatcc ggctcccatc tcgtagcaca
     7081 acgtccgaaa aggtctgtct gtaatgccag ccatgggcgc tgcgatcagg cgatttctga
     7141 gctgatattg tccgatgcgc atgagttaag aaatgaccat actgtgactg caaggcggcg
     7201 tatattacgc attttttgca cgagatgaaa ggccaaactt tgaccaatcc tctgagatgg
     7261 atcaaagaat tgcatttaaa atgagcgtgg tgagataatt actcataaaa atcatcatat
     7321 tagaaaatag tgactaaaat ttacactcaa agaaatttga gtaagttctc aatttttctt
     7381 tatgaatgaa aattttggca cgcaaatttg cgtaaataac cggcaaattt acgtgccttt
     7441 tgtgagcttg ctcgcacttc gccccgcgtc accctacggc gatgcgaagg ttaattctta
     7501 cgaccggtaa tgcggcacca ctcttctttt tccacgaccg ggtccagtgc gaagctgtcg
     7561 acataagctt cacaaacgct ctctgcctgg cttgccagaa taccggaaag gcccagcaaa
     7621 ccgcctgaaa ccggcaggac gctgattaac ggtgccagtt cacgtaatgg gcctgcaagg
     7681 atgttagcga ccaccacgtc ggctttcatt tcttctggct gatctttcgg taagtagagt
     7741 tccagacggt cagaaacgcc attacgttcg gcgttgtcgc ggctagcctg aatcgcctgc
     7801 ggatcgatat caataccaat ggcttttgct gcacccagtt tcagcgccgc gatagccaga
     7861 atgccggaac cacagccaaa gtcgatgact attttaccgg ttaaatcgag gctgtcgagc
     7921 cactgcaggc acagagaggt agttggatgg gtacccgtac caaacgccag ccctggatct
     7981 aacatcacgt tgacggcgtt ttcgtccggc acatcacgcc agctagggca gatccacagt
     8041 cgttcaccaa agcgcatcgg gtggaaatta tccatccatt cgcgctccca gtctttatct
     8101 tccagttgtt cgattttatg cgcgaagcct gcgccgagca gcggatggtt ttccagaatc
     8161 gccaccacgt cgttcatatc ggtttcagcg tcgaacagac caatcacatc ggtgtcgccc
     8221 cacaggcgcg tttcgcccgg cagcggctca aatactggcg tatcgtgggt atcctgaaaa
     8281 gtgatagaaa cggcacccgc ttccatcagc gcatcgctaa gatcttcagc gttcgcgccg
     8341 gtggtgttca gtttcagttg gatccaaggc atggcaaaac tctttattta tcagtagtca
     8401 aaacggtagc ttgcgggacg gatgtaccga aacggtttcc gaccaggaaa gccagcaaac
     8461 ttagtagtaa cgagggcacg atagggtgga agcccaggta ctgaatattc agcgtcgcga
     8521 gtacggcata cagcacgccg ccaacgatca tcgcacttag cgcgcctttg gcgttggcgc
     8581 gttcccagta aagacccagc accagcggcc acaggaaaac ggcttccagc ccaccgaagg
     8641 ccagcaaatt cagccagatg atcatttctg gcggcttcca ggcggcaagc agcagcaacg
     8701 cgccgagaac taacgtaatt accgccgaca tccgcttcag acgcgtctcg ttttgcattt
     8761 gatccggacg gatattcaga tagagatctt taatgatcgt agcggaactt tgtagcaatt
     8821 gggcgttaat tgtcgacatg atcgcagcca taggtgcagc cagaaagatc ccggcagcaa
     8881 acggtggcag cacttttacc attaacgtcg ggatcaccag atccggtacg gtgagatcgg
     8941 ggatcactgc acgacctaac gctccggcta ggtgcatacc gaacatcagg attgcgacta
     9001 caatcgtacc gatgatgatc ccccggtgta ccgctttgct gtctttataa gagatacagc
     9061 gcaccgcagt atgcggcagg ccaatcacgc caaagcacac cagtacccag aacgacgtca
     9121 taaaggcagg cgacagaata tcgtcagcgc cttgtggcgt aaccagttgc ggatcgatgg
     9181 tttgcaaggt ctgtactgcg ttgcttaagc cgccagcggc atgtaccacg ccaataagca
     9241 gcacaacggt gccaatcagc atcacaagcc cttgcatggt gtcgttcagc acgctggcgc
     9301 gaaagccgcc aaaggcggta tacaacgcaa tgctgatacc gaaaatcagc agcccggttt
     9361 cataaggaat acccgccgcg gtttccagca ggcgcgcacc gccgataaac tgcacggtca
     9421 ttgccccaac gaacgcaacc agcaaactca aactcgccag ccacaccaga agacgactct
     9481 ggtagcgggc aaacagcatg tcgttcagcg tcactgcatt gtagcggcgc gcaagaatcg
     9541 caaacttctt gccgagaata ccgagtgaaa gccagactgc aggaagctga atcatcgcca
     9601 gcaataccca gcccagcccg tatttataag cagctcctgg cccgccgata aacgaactgg
     9661 cactgatata ggtcgcggtg agcgtcatcg ccagcacaat accgcccata gagcggctgc
     9721 cgaggaaata ctcattaagg aaggtgccgg tgctccgttt acgcatcgca taaaccgaga
     9781 taccgaacac caccaccaga taggcgacca gcggtagaat tacttcaagc tgcatcgtca
     9841 tcctccagtg ggatatcgcg atagataaat ttcaccatcg cccagcacag tccaataaac
     9901 agtagcggcg tcaggatgca ggccatctca aaccagcgcg gaaagccggt aaaaccgggg
     9961 gcaacgccag gtaagtaagc ggctactaac caaactgcca gatacaaaag ggtcagcccc
    10021 agcgcccagc gcgcctcttt atgggcctga acaaaacgag tgtccatttt ttgtccctga
    10081 tgggtgaaga aagcgaggat tgtaccttat gggggttgtc gatccccagt aataaaaaag
    10141 gccggaaaat ccggcctttt ggcgcttttg cagtcttatt tttcctgaag accgagtttt
    10201 ttctccagat agtggatgtt agtgccacca tgctggaagt tctcgtcatt catgatgcgg
    10261 atctgcagat caacgttggt tttgataccg tcgatgatca gctcctgcag cgcattcttc
    10321 atgcgggcaa tcgccacgtc acggttttca ccgtagcaaa tcagcttacc gatcattgag
    10381 tcatagtacg gcggtacggt gtagcccgcg tagatatgag actcccaacg tacgccaaaa
    10441 ccgccaggtg cgtggaaacg ggtgattttg cctgggcttg gcaggaaggt gttcggatct
    10501 tcggcgttga tacgacattc caccgcatgg ccgcgaacgt gaacttcttc ttgcttgatc
    10561 gacagcggtt gaccggcagc gatacggagc tgttctttga tcaggtcaac gccagtgatc
    10621 atttcggtaa ccgggtgttc tacctgaata cgggtgttca tttcgatgaa atagaactcg
    10681 ccgttttcga acagaaactc gaaagtacct gcaccgcgat agccgatatc aacacacgct
    10741 ttagcgcaac gttcgccgat gtagcgacgc agttccgggg taatgcccgg tgctggcgct
    10801 tcttcgacca ctttctggtg gcggcgctgc atggagcagt cacgttccgc cagatagata
    10861 gcgttgcccc gaccgtcagc cagtacctga atctcgacgt ggcgaggatt ttccaggtat
    10921 ttctccatgt aaaccatatc gttgctgaaa gcagctttcg cttccgcacg ggtcatggag
    10981 atggattgtg ccagttcagc gtcgccgcgc actacgcgca taccgcgacc accgccgccg
    11041 ccggaggctt tgataatcac cggataacca atgcgtttag caatggcacg gtttttatcc
    11101 atatcgtcgc ccaacgggcc gtcagaaccc ggtacgcaag ggacgcccgc ttttttcatc
    11161 gccgcgattg cggatacttt gtcgcccatc aggcgaatgg tttctgcttt cgggccgatg
    11221 aagataaagc cggagcgttc aacctgctcg gcaaagttgg cgttctcgga gaggaagccg
    11281 taacccggat ggattgctac tgcgccggtg atttcagcgg cgctgatgat tgccgggatg
    11341 ttcagataac tttttactga cggagcaggg ccaatacaga ccgtttcatc tgccagtaat
    11401 acgtgtttta gatcgcgatc cgcgctggag tgcacagcga cagtcttgat gcccagttct
    11461 ttacaggcac gaagaatacg caatgcaatc tcgccgcggt tggcaataac aattttatcc
    11521 agcatgttcg cctcgttact cgatgacgac cagcggctcg tcaaattcta ccggttgtcc
    11581 actttcgacc agaattgctt tcacggtacc ggatttgtcc gcttcgatct ggttcatcat
    11641 tttcatggct tcaacgatgc acagggtatc gcccacgttg actttctgac ccacttcgat
    11701 gaacgctttt gcgtccgggc ttggggtgcg gtagaaagta ccaaccatcg gggaacgtac
    11761 gatgtgacca ctgatttccg ctgctgctgg cgcttccatg gaaggaacgg tcgccggagc
    11821 ggctgcgtta gattgagctg gctgctgcat cattggtgca gcgtaagctt gttgcatcac
    11881 agggaaactt gcggcaggag ctgcacggct aatgcgtact gactcttcgc cttcagaaat
    11941 ttccagttcg gagatgcctg attcttcaac cagctcgatc agttttttaa tcttacgaat
    12001 atccatgagt gggttccgta ctctttgttt agtgtgattg tgacaggcgt ttcaccgccg
    12061 tctgtaaagc gtatgccccg acagctgtat gcatagcgat aaattccagc aggccgggac
    12121 gccggtctat tttgccccag agtatcaatg ttagacttga cggacattgt gcagcgatct
    12181 gctgctatcc tccggcaaaa aacaaaatat accttcgttg cgccaatgtc accttttccg
    12241 cagcgcaaaa actgcgtcag ggaggacgag gtcgcacatt ataacgattt cgtagcaatt
    12301 ggcagctaaa tactggtctc atcagggaag ataatcaaca gctaacatgt aaataacctt
    12361 caacaccgtg taatttgcaa caagccgcac aattcacgaa attagcgcca tcatcgacgg
    12421 aacttcttat aacgtaaggt gaaaagctgt aaagccagcc ctgcgaagat gaggggctgc
    12481 aatgagataa tcttcacaga ccacaaataa tgtagggatg ccaggatcgc gacaagatag
    12541 acgacattac gcagcggttg ctggggatta cccaattttc actgcatcgt ctggatagat
    12601 gtgaatgcta aagcacgcaa aatcaaccga ctgacaattc tcagcattaa ataggttcga
    12661 gtggttaatt tgtagtcaaa taaaatcaga ttattcaccc catggtacca gaaatcagta
    12721 acaggttaag cgtagtgtcg cccaggcaaa gcaccataaa cctaacaggc gacggaggcg
    12781 tatcaataat ggctgtttag aggagtatca atgcgcatca atttgtggtc agtcgaaatc
    12841 atgactttcc agtttggatg atgttgtaca acttcctgac caaggtgttc gacgaaagct
    12901 gtccggctaa ccgcttttcc cgttaaccga ttgaataatg atgtcaaaat gagttccctc
    12961 tcttattatt ccttgctaaa tggttagtta accttcacca gcgtgcgacc ctggatctgg
    13021 ttattaatga tggcctcggc aaagttcggt gcctctgaca gagatatctc ttttgccgcc
    13081 tgggtataga atgattccgg taaatcggcg accagtcgct gccaggcttg tgcgcggcgt
    13141 tctggtggcg tcattactga atccacccct tgcaaacgga cattacgcag aataaatggc
    13201 atgaccgtgg ttggcagagt aaaaccaccc gccagaccac aggccgccac gcagccgccg
    13261 taattcattt gcgccagcac ttttgccagc actttgtcgc caacagtgtc aattgcccca
    13321 gcccagactt gtttttccag aggacgggat tcggcaaact catcacgagg gagaatacgg
    13381 ctggcaccta aacttttcag atattcatgg gtactttcgc gaccggaaac ggcaacgacc
    13441 tgataaccca acttatgcag cagcgccacg gcggtactgc cgacgccacc actggcaccc
    13501 gtcacgacaa tctccccgtc ctgcgggcga acaccggcat cttccagcgc catcacacac
    13561 agcatggcgg taaaaccggc agtaccgata atcattgctt tacgcgcgtc cagcccttgc
    13621 ggcatggcaa ccagccagtc acctttcact cgcgcctgct ccgccagccc accccagtgg
    13681 ttttcaccaa cgccccagcc agtgagtaac acctcctgac cggcatgaaa acgcggatct
    13741 tcgctggtgc gtacagttcc ggcaaaatcg atcccaggaa tcatcggaaa attacggatg
    13801 atttttccct taccggtaat cgccagcgca tctttatagt tcaggctcga ccagtgaaca
    13861 tcgaccgtga catcgccctc cggcaggcga ctttcgtcca gagtctgtac tgatgcgaga
    13921 gttttgccgt cctgctgttc taaaagtaac gcctgcataa gtggtcctca tgtgcatgat
    13981 ggattggaaa ataatttctg aagattatac tcgctaatgg aaaagcaaag ccgatgaagc
    14041 gcaataaatt gcgagataaa tctgatttgc tagtatgccc gcttcctcac tatcggagtt
    14101 aacacaagga tgagattaac gacgaaattt tcggcctttg ttacgctgct caccgggtta
    14161 acaatttttg tgactttgct gggctgttcg ctaagtttct acaacgcaat tcagtataag
    14221 tttagtcatc gcgttcaggc ggtggcgacg gcgattgata cccaccttgt gtcgaatgac
    14281 ttcagcacat taaggccaca aattaccgaa ttaatgatgt cggcagatat cgttcgtgta
    14341 gacctgctcc atggtgataa acaggtttat accctggcca gaaatggtag ttatcgtccg
    14401 gttggctcca gcgatctgtt tcgcgaactg agtgttccgt tgataaagca tccggggatg
    14461 tcgctgcgtc tggtttatca ggatccgatg ggcaactatt tccattcttt aatgactacc
    14521 gcgccgctca cgggggcgat tggctttatc attgttatgc tcttcctggc ggtacgctgg
    14581 ttacaacggc aacttgccgg gcaagaattg ctggaaaccc gggctactcg tatcttaaac
    14641 ggtgagcgtg gctctaatgt gttgggaacc atctatgaat ggccgcccag aaccagcagt
    14701 gcgctagata cgctgcttcg tgaaattcag aacgcacgcg aacaacacag ccgtcttgat
    14761 acgctgatcc gctcttatgc cgcccaggac gtgaaaaccg gcctcaataa ccgactcttt
    14821 ttcgataatc agttagcaac gttactggaa gatcaggaga aagtaggtac ccacgggatc
    14881 gtgatgatga ttcgtctgcc ggatttcaat atgttgagcg atacttgggg gcacagccag
    14941 gttgaagaac agttcttcac tctgactaat ctgctgtcga catttatgat gcgctaccct
    15001 ggcgcactgc tggcgcgtta ccaccgcagt gattttgctg cgctgttacc gcaccggacg
    15061 ttaaaagagg cagagagcat cgccggtcag ttaatcaaag ccgtcgatac cttgccgaac
    15121 aataaaatgc tcgatcgcga cgatatgatc cacattggta tctgcgcctg gcgtagtggt
    15181 caggataccg agcaggtaat ggaacatgca gagtctgcca cgcgtaatgc gggattgcag
    15241 ggcggcaata gctgggctat ttacgatgac tcgttgcctg aaaaaggacg cggtaatgtt
    15301 cgctggcgta cgcttatcga gcaaatgctc agtcgcggcg gcccgcgcct ttatcaaaaa
    15361 ccggcggtta ctcgcgaagg tcaggttcat catcgcgaac tcatgtgccg catcttcgat
    15421 ggtaatgaag aggttagctc ggcggagtat atgccgatgg tcttgcagtt tggcttatcg
    15481 gaagagtatg accgtctgca aatcagccgt cttattccac tattgcgtta ctggccagag
    15541 gaaaatctgg cgattcaggt taccgttgag tcgctgattc gcccgcgttt tcagcgttgg
    15601 ctgcgcgata cgttaatgca atgtgaaaaa tcacaacgaa aacgcataat tattgaactt
    15661 gcagaggccg atgtaggtca acatatcagt cgtttacaac ctgttattcg tttagtgaat
    15721 gctttagggg tacgggtagc cgtcaaccag gctggtttga cgctggtaag caccagttgg
    15781 atcaaagaac ttaatgttga gttactcaag ctccatccgg ggctggtcag aaacattgag
    15841 aagcgaacgg agaaccagct gctggttcaa agcctggtgg aagcctgctc cgggaccagc
    15901 acccacgttt acgccaccgg cgtgcgttcg cgaagcgagt ggcagaccct gattcagcgc
    15961 ggtgttacag gcgggcaagg ggattttttc gcgtcctcac agccacttga tactaacgtg
    16021 aaaaaatatt cacaaagata ctcggtttaa cctgccgttt aatccgtttt cacgtagaat
    16081 aacgcgcgct gcgtctcatg ggagtgtgct tgtctgctcg ccagattgtt gcagcacata
    16141 tgcagatgaa tgaccttacg cggttgcaaa caggcgagga atgctgctga tgcattaagc
    16201 ctttctggac tcaggcagag atttgtaaca aaggaaacga actgcactaa ttttcaccgt
    16261 agcagatgat ttttgcgcct tgtcgctgct gcgtgtggtt ggtaaagtaa gcggattttc
    16321 ttttccgccc cagctttcag gattatccct tagtatgttg aaaaaatttc gtggcatgtt
    16381 ttccaatgac ttgtccattg acctgggtac tgcgaatacc ctcatttatg taaaaggaca
    16441 aggcatcgta ttgaatgagc cttccgtggt ggccattcgt caggatcgtg ccggttcacc
    16501 gaaaagcgta gctgcagtag gtcatgacgc gaagcagatg ctgggccgta cgccgggcaa
    16561 tattgctgcc attcgcccaa tgaaagacgg cgttatcgcc gacttcttcg tgactgaaaa
    16621 aatgctccag cacttcatca aacaagtgca cagcaacagc tttatgcgtc caagcccgcg
    16681 cgttctggtt tgtgtaccgg ttggcgcgac ccaggttgaa cgccgcgcaa ttcgtgaatc
    16741 cgcgcagggc gctggtgccc gtgaagtctt cctgattgaa gaaccgatgg ctgccgcaat
    16801 tggtgctggc ctgccggttt ctgaagcgac cggttctatg gtggttgata tcggtggtgg
    16861 taccactgaa gttgctgtta tctccttgaa cggcgtggtt tactcctctt ccgtgcgcat
    16921 tggtggtgat cgtttcgacg aagctatcat caactatgtg cgtcgtaatt acggttctct
    16981 gatcggtgaa gccaccgcag aacgtatcaa gcacgaaatc ggttcggctt acccgggcga
    17041 tgaagtccgt gaaatcgaag ttcgtggccg taacctggca gaaggtgttc cacgcggttt
    17101 taccctgaac tccaacgaaa tcctcgaagc actgcaggaa ccgctgaccg gtattgtgag
    17161 cgcggtaatg gttgcactgg aacagtgccc gccggaactg gcttccgaca tctccgagcg
    17221 cggcatggtg ctcaccggtg gtggcgcact gctgcgtaac cttgaccgtt tgttaatgga
    17281 agaaaccggc attccagtag ttgttgctga agacccgctg acctgtgtgg cgcgcggtgg
    17341 cggcaaagcg ctggaaatga tcgacatgca cggcggcgac ctgttcagcg aagagtaatc
    17401 ggatgcaggc aggggaaatg tctgtttacc ctgcctggtc tgatacgaga atacgcataa
    17461 cttatgaagc caatttttag ccgtggcccg tcgctacaga ttcgccttat tctggcggtg
    17521 ctggtggcgc tcggcattat tattgccgac agccgcctgg ggacgttcag tcaaatccgt
    17581 acttatatgg ataccgccgt cagtcctttc tactttgttt ccaatgctcc tcgtgaattg
    17641 ctggatggcg tatcgcagac gctggcctcg cgtgaccaac tagaacttga aaaccgggcg
    17701 ttacgtcagg aactgttgct gaaaaacagt gaactgctga tgcttggaca atacaaacag
    17761 gagaacgcgc gtctgcgcga gctgctgggt tccccgctgc gtcaggatga gcagaaaatg
    17821 gtgactcagg ttatctccac ggttaacgat ccttatagcg atcaagttgt tatcgataaa
    17881 ggtagcgtta atggcgttta tgaaggccag ccggtcatca gcgacaaagg tgttgttggt
    17941 caggtggtgg ccgtcgctaa actgaccagt cgcgtgctgc tgatttgtga tgcgacccac
    18001 gcgctgccaa tccaggtgct gcgcaacgat atccgcgtaa ttgcagccgg taacggttgt
    18061 acggatgatt tgcagcttga gcatctgcca gcgaatacgg atattcgtgt tggtgatgtg
    18121 ctggtgacct ccggtctggg cggtcgtttc ccggaaggct atccggtcgc ggttgtctct
    18181 tccgtaaaac tcgataccca gcgcgcttat actgtgattc aggcgcgtcc gactgcagga
    18241 ctgcaacgtt tgcgttatct gctgctgctg tggggcgcgg atcgtaacgg cgctaacccg
    18301 atgacgccgg aagaggtgca tcgtgttgct aatgaacgtc tgatgcagat gatgccgcag
    18361 gtattgcctt cgccagacgc gatggggcca aagttacctg aaccggcaac ggggatcgct
    18421 cagccgactc cgcagcaacc gacgacagga aatgcagcta ctgcgcctgc tgcgcccaca
    18481 cagcctgctg ctaatcgctc tccacaaagg gctacgccgc cgcaaagtgg tgctcaaccg
    18541 cctgcgcgtg cgccgggagg gcaatagtgg cgagctatcg tagccaggga cgttgggtaa
    18601 tctggctctc tttcctcatt gcgctgttgc tgcaaatcat gccctggccg gataacctga
    18661 ttgttttccg gccaaactgg gtgttactca tcttgttgta ttggatcctg gccttgcctc
    18721 atcgcgtaaa tgtgggcaca ggttttgtga tgggtgccat actggatctg atcagcggct
    18781 cgacgcttgg cgtacgcgta ttggcgatga gcatcattgc ttacctggtg gcgctgaaat
    18841 accagctttt ccgcaacctc gcattatggc agcaggcgct ggtcgtcatg ttgctttcgc
    18901 tggtggtgga tattattgtt ttctgggcag agtttttagt gattaacgtc tctttcagac
    18961 cggaagtgtt ctggagtagt gtagtcaatg gggtgctctg gccgtggatt ttcttgctga
    19021 tgcgtaaagt ccgtcagcag tttgcagtgc aataaaggtt tctatgactt ctctgtattt
    19081 agcttccggt tctccgcgtc gtcaggagtt acttgcgcaa cttggtgtga cctttgaacg
    19141 tattgttacg ggcattgagg agcagcgtca gccgcaggag agcgcgcagc agtatgttgt
    19201 gcgtctggcg cgcgagaaag cacaggcggg tgtcgcgcaa acggcgcagg atctcccggt
    19261 gctgggtgcg gatactatcg ttatcctgaa tggagaagtg ctggagaaac cgcgcgacgc
    19321 agagcatgcg gcgcagatgt tgcgcaaatt atcgggtcag acccatcagg tgatgacggc
    19381 agtggcgttg gccgacagcc agcacattct cgattgcctg gtggtcaccg atgtgacttt
    19441 cagaacgtta acagacgaag acatcgcggg ctatgtcgcc agcggtgaac cgttagataa
    19501 agcaggtgca tacggtattc aggggctggg tggctgtttt gtcaggaaga taaatggcag
    19561 ctatcacgcc gtagtcggct tacctctggt tgaaacgtat gaattattaa gtaattttaa
    19621 cgcactgcgt gagaaaaggg ataaacatga cggctgaatt gttagtaaac gtaacgcctt
    19681 cggaaacgcg agtggcgtat attgatggcg gtattctgca ggaaattcat attgaacgtg
    19741 aggcgcgacg cggaatagta ggcaatatct acaagggtcg tgtaagtcgt gtacttccgg
    19801 gtatgcaggc ggcttttgta gatattgggc tggataaagc cgcgtttctt catgcatccg
    19861 atatcatgcc gcacaccgaa tgtgtggcgg gtgaagaaca aaagcaattc acggtgcgcg
    19921 acatctcgga actggttcgt caggggcaag atctgatggt gcaggtggtg aaagatccgc
    19981 ttggcactaa aggtgcgcgc ctgaccaccg atatcacgct gccttctcgc tatctggtct
    20041 ttatgccagg ggcttctcac gttggggttt cccaacgtat tgaaagcgaa tcagaacgtg
    20101 aacgcctgaa aaaagtggtc gcagagtatt gcgacgagca gggcgggttt atcatccgta
    20161 ccgcagcgga aggggttggc gaggctgaac tggcctccga tgccgcttat ctgaaacgcg
    20221 tctggaccaa agttatggag cgtaaaaagc gcccgcagac ccgttatcag ctgtacggcg
    20281 aactggcgct ggctcagcgt gttctgcgtg atttcgccga cgccgaactg gaccgcattc
    20341 gcgttgactc acgcctgact tacgaagcat tacttgagtt cacctcggag tacattcccg
    20401 agatgaccag caagctggag cattacaccg gacgccagcc gattttcgat ctctttgatg
    20461 tcgaaaacga aatccagcga gcgctggaac gcaaagtaga actgaaatcc ggtggttatc
    20521 tcattatcga ccagaccgaa gcgatgacca ccgtggacat caataccgga gcgtttgtcg
    20581 gtcatcgcaa tctggacgac accattttca ataccaatat tgaagcgacg caggctatcg
    20641 ctcgccagtt acggttgcgt aatctgggcg ggattatcat tattgatttc atcgatatga
    20701 ataatgaaga tcaccgccgc cgcgtgctgc actcgctgga gcaggcgttg agcaaagacc
    20761 gggtgaaaac cagcgttaat ggtttttcgg cgctggggct ggtggagatg acgcgtaaac
    20821 gcacccgcga aagcattgag cacgtactgt gtaacgaatg cccaacctgc cacggtcgcg
    20881 gaacggtgaa aaccgtggaa acggtatgct atgaaatcat gcgcgagatt gttcgtgtcc
    20941 accatgctta cgactccgac cgtttcctgg tctatgcttc tccggcagta gccgaagcct
    21001 tgaaaggcga agagtcacac tcgctggcgg aagtggaaat tttcgttggc aaacaggtta
    21061 aagtacaaat tgaaccgctc tataaccagg agcagtttga cgtcgtaatg atgtaaacag
    21121 atgctgggcc gccatccggc aaagggtttt tgagtcacat ttttagcaga caaggagtga
    21181 cgggtgaggc gattgccggg gattttactg cttactggag ccgcgctcgt tgtgatcgct
    21241 gccctgctgg ttagcggcct gcgtattgct ttaccgcatc ttgacgcctg gcgtccggaa
    21301 atcctcaaca aaatagaatc cgcgactggc atgccggtag aagccagtca gctctcagcc
    21361 agctggcaga attttggccc gacgcttgaa gcacacgaca tccgtgcaga actaaaagat
    21421 ggcggcgaat tttcggttaa acgcgttact ctggcgctgg atgtctggca gagcctgtta
    21481 catatgcgct ggcagtttcg cgacctcact ttctggcagc tgcgctttcg caccaacact
    21541 cctatcacca gcggtggtag cgatgacagt ctggaagcca gtcacatcag cgatctgttt
    21601 cttcgtcaat ttgaccattt cgatctccgc gacagtgaag tcagtttcct gacgccatcc
    21661 ggtcagcgtg ccgagctggc gatcccacaa ctcacctggc tgaacgatcc acgtcgacac
    21721 cgtgcggaag gcctggtaag cctctccagc cttaccggac agcacggcgt gatgcaggtg
    21781 cgcatggatt tgcgcgatga tgaggggttg ttaagcaatg gtcgcgtctg gctacaggcg
    21841 gatgacatcg acctgaagcc gtggctcggt aaatggatgc aggacaatat tgctctggaa
    21901 acggcacagt tctcccttga aggctggatg acgatcgaca aaggcgatgt aaccggcggt
    21961 gacgtctggc tgaaacaggg cggtgccagc tggttgggcg agaagcaaac gcatacgctg
    22021 tcggtggata atctgaccgc gcatattacg cgtgaaaatc cgggctggca gttctctatt
    22081 cccgatacac ggatcacgat ggacggcaaa ccctggccga gcggagcatt gacgctggcc
    22141 tggataccgg aacaggacgt tggcggcaaa gacaataaac gcagtgacga actccggatt
    22201 cgcgccagta atctggagct ggcaggcctg gagggcatac gcccgctggc cgcgaaactt
    22261 tcacctgcac tgggtgatgt ttggcgctcc acacaaccga gcggcaagat taacactctg
    22321 gcgctggata tcccgcttca ggcggcagac aagacccgtt ttcaggcatc gtggagcgat
    22381 ctggcctgga agcaatggaa attattaccg ggtgcggaac acttctccgg gacgctttcc
    22441 ggcagcgttg aaaatggttt gcttaccgcg tcgatgaagc aggcaaagat gccttacgaa
    22501 acggtattcc gtgcgccact ggaaatcgcc gacggccagg caactataag ctggctgaac
    22561 aatgacaaag gtttccagct ggatgggcgt aatattgacg ttaaagccaa agccgtccat
    22621 gcgcgcggcg gttttcgtta cctgcaacct gctaacgatg aaccctggct gggtattctg
    22681 gctggcatca gtaccgatga tggttcacaa gcctggcact atttcccgga aaacttgatg
    22741 ggtaaagacc tggttgatta cttaagtggc gcgattcagg gcggtgaagc ggataacgcg
    22801 acgctggttt atggtggcaa tccgcaactc ttcccctata aacacaacga aggtcagttt
    22861 gaagtgctgg tgccgctgcg caacgcgaag tttgccttcc agccggactg gcctgcatta
    22921 actaaccttg atattgaact ggactttatt aacgacggtt tatggatgaa aaccgatggc
    22981 gttaatctgg gcggcgtgcg cgcgagtaat ctcaccgcag tgatccctga ctactcgaaa
    23041 gaaaaactgc tgattgacgc tgacattaaa ggtccgggta aagccgttgg cccttacttt
    23101 gatgagacac cgctgaaaga ttctctgggt gcgaccctgc aagaactcca gctcgacggc
    23161 gatgtgaatg ctcgcttaca tcttgatatc ccgctgaacg gcgagctggt aaccgcgaaa
    23221 ggtgaagtga cgctgcgtaa taacagtctg tttatcaaac cactcgacag caccctgaaa
    23281 aatttgagcg gtaaattcag ctttatcaat ggcgatctgc aaagtgaacc actgacagca
    23341 agctggttta atcagccgtt gaacgtggat ttttccacca aagaaggggc aaaagcctac
    23401 caggttgcgg tgaatctcaa cggtaactgg caaccggcga aaaccggcgt tctgcctgaa
    23461 gcggtgaacg aagcattgag tggcagcgtg gcgtgggatg gtaaagtggg cattgatctg
    23521 ccttatcatg ctggcgcgac ctataacgta gagctgaacg gcgatctgaa taatgtgagt
    23581 agtcacttac cttcaccgtt agccaaacct gcgggtgaac cactagcggt aaacgttaag
    23641 gttgatggca atctcaacag ctttgaatta accggacagg ctggtgcgga taaccatttc
    23701 aatagccgct ggttgcttgg tcaaaagctg acgctcgatc gtgctatttg ggcggcagac
    23761 agtaaaacgc tcccgccgtt gccggaacaa agtggcgttg aactcaatat gccgccgatg
    23821 aatggtgccg agtggctggc cctgttccag aaaggcgctg cggagagtgt cggtggtgca
    23881 gcgagtttcc cacaatacat aacgttacgt acgcctatgt tgtcactggg aaatcagcaa
    23941 tggaataacc tgagtattgt ttcgcaaccg acggcaaatg gcaccctggt tgaggcgcaa
    24001 gggcgtgaaa tcaacgccac gctagcgatg cgtaataacg cgccgtggct ggcgaatatc
    24061 aaatatcttt attacaaccc gagcgtggcg aaaactcgtg gtgattcaac gccgtcatca
    24121 cctttcccga caacggagcg cattaacttc cgtggctggc cggacgcaca aatacgatgc
    24181 acagagtgct ggttctgggg gcaaaaattc ggtcgcattg acagtgatat caccatttct
    24241 ggcgatacgt taacgctgac caatggactg attgatactg gtttctcgcg gcttactgcc
    24301 gatggtgaat gggttaataa tccggggaat gaacgtacct cgctgaaagg aaaactgcgc
    24361 gggcagaaaa ttgatgccgc cgcagaattt tttggtgtca cgacgcccat acgccagtcg
    24421 tcatttaatg tggattacga tttacactgg cgcaaagcac cctggcagcc agatgaagcg
    24481 acgttgaatg gcatcattca tactcaactg ggtaaaggcg aaattaccga aatcaatacc
    24541 ggacatgccg ggcaattgct gcgcttattg agcgtagatg ccctgatgcg taagctgcgt
    24601 tttgatttca gagacacttt tggcgaaggg ttctattttg actccattcg cagcaccgcg
    24661 tggattaaag acggggttat gcacaccgac gacacgctgg tggatggcct ggaggcggat
    24721 atcgccatga aagggtcggt aaatctggta cgtcgcgacc tgaatatgga agcggttgtc
    24781 gcaccagaga tttctgcgac ggtgggcgtg gctgcggctt ttgcggttaa ccccattgtt
    24841 ggcgcggcag tttttgccgc tagtaaagtg ctggggccgc tgtggagcaa agtctccatt
    24901 ttgcgctatc acatttcggg tccgctggac gatccgcaaa tcaacgaagt gttgcgccaa
    24961 ccgcgtaaag aaaaagcgca atgatttgac gagggcgcgt aattgcccca atctcatatg
    25021 ataatcgttg ccaaaggcca acgagccaga acataaccgt aggccggata gggcgttcat
    25081 gccgcatccg gcagccgtaa aaaatcctct actgcagtaa ctaacgagta gcaaaaacga
    25141 tgagtctgaa cctggtaagt gaacaattgc tagcggcgaa cagcctgaaa catcaggact
    25201 tgttcgcgat cctcggtcaa ctggccgaac gtcgccttga ttatggcgat ctctattttc
    25261 agtcgagcta tcacgaatcc tgggttttag aagaccgcat tattaaagat ggttcttaca
    25321 acatcgatca gggcgttggc gtgcgtgcaa tcagcggtga aaaaaccgga tttgcttacg
    25381 ctgaccaaat cagcctgctg gcgctggaac agagtgcgca agcggcgcgc accatcgtcc
    25441 gtgatagtgg tgatggcaaa gtacagacgc tgggcgcggt agagcatagc ccgttgtata
    25501 cctcggtaga tccgctgcaa agcatgagcc gtgaagagaa gctggatatc ctgcgtcgcg
    25561 tcgataaggt tgctcgcgaa gcggacaagc gcgtacagga agtgactgcc agcctcagtg
    25621 gtgtctatga attaattctg gttgcggcca ccgacggcac gctagcggcg gatgtccgtc
    25681 cgctggtgcg tctttccgtg agcgttctgg tcgaagaaga tggcaaacgc gaacgcggtg
    25741 ccagtggcgg cggcggtcgt tttggttatg agttcttcct tgccgatctt gacggcgaag
    25801 tccgtgcgga tgcatgggca aaagaagcag tgcgtatggc gctggtcaat ctttctgccg
    25861 ttgctgcacc agcgggcacc atgccggtag tacttggcgc aggttggccg ggcgtgctgt
    25921 tgcatgaagc ggtaggtcac ggtctggaag gcgacttcaa ccgccgcggc acttcagtat
    25981 ttagtgggca ggtcggggag ctggtggctt cagaactgtg taccgtggtt gatgacggca
    26041 cgatggtcga tcgccgtggt tcggtggcga ttgatgacga aggtacgcca ggccagtaca
    26101 acgtgctgat tgagaacggc attctgaaag gctacatgca ggataaactc aacgcgcgtt
    26161 tgatggggat gacgccgact ggcaacggtc gccgtgaatc ctacgcccat ctgcccatgc
    26221 cgcgtatgac taacacctat atgctgccgg gtaaatcgac cccgcaggaa attattgaat
    26281 ccgttgagta cggtatctat gcaccgaact ttggtggcgg tcaggtggat atcacctccg
    26341 gcaaattcgt tttctccact tcagaagcat atctgattga aaacggtaaa gtaacgaagc
    26401 cggtgaaagg cgcaacgttg attggttccg gtatcgaaac catgcagcag atttcgatgg
    26461 ttggcaacga cctgaaactg gataacggcg tgggtgtctg cggtaaagaa gggcaaagtt
    26521 tgccggttgg cgtgggccag ccaacgctga aagttgataa cctgactgtt ggcggtactg
    26581 cgtaataatt tcattatttt cagaaggata attaatcttt ctacgtgcac gaacggtccc
    26641 ctcgctcctc tggggttagg gtgaggggaa cccgttggca caggtttgta gaacgtaaca
    26701 gtacaatatg aattacttct ctttcccgcg cccgtgcatc tcctgaaaca atttaccgac
    26761 ctcaacaaaa taatccgtca gcgagttgat cacgacctgt accttcagcg gcagcttatc
    26821 tttttcggta tataacgcat aaaccgggcg tggatctgac tgataacgcg gcagcaggat
    26881 ctccagctca ccgcgattga tctcgttgat cacccacatc agcggcacgt aggcgatccc
    26941 ggcacccgcc gtcagccagc gcaccagcgt catcggatca ttagtcacaa atcttccttg
    27001 tgggatcagg cgagtcgaga tcccttccgg tgcgatcagt tcaaattcat tgtcgggccg
    27061 cacgctgtat tcaagccatg aatgactact caaatcggcg ggtttttccg gtatgccgta
    27121 ttgtgtgaga tagcttttcg cggcgcacac caccatcggc atcgcgccca gacggcggga
    27181 aaacaggctg gaattccggt aaccagattg acgctcaaac ctgggtattc tttcagcatt
    27241 tttgctgtca gcccggcgag aacattttgt gccatagttg aagaacagcc aatgcgcagc
    27301 gtcccgatgg gggtgttatt gaaggcatac agttgctcat gaacatcctg cacttcatga
    27361 agcatccgac ggcagccctg gtagtaaatt ctgccggctt cggtcaggcc aatgctgcgt
    27421 gtgctacggt ttaacagctt tacctgcaac tcatcttcca gttttgacac cgtctgactg
    27481 atggacgaaa cgctcatctg tagctgtctg gcggcggcgg taaaagagcc aaattcaact
    27541 actttggcaa acaccgacat gcgttttagt cgttccatta ttcactctga cttaaaagtg
    27601 atttagatca cataatatag ataacagcat aacagttacg ctaatatatt aaatatcaat
    27661 ctacagcaat gttgctctcg cccggctttc catgcccttc tctgcggcga cagatgctga
    27721 aaataataac gcctgctctc tctttacaac caaggtcaac atgagtctgt ttcccgttat
    27781 cgtggtgttt ggactgtcct tcccaccgat attttttgaa ttgcttttat cactggcgat
    27841 tttctggctg gtgcgccggg tacttgtgcc aacaggtatc tacgactttg tctggcatcc
    27901 ggcgttgttc aacaccgcgc tctattgctg cttgttttat ttgatatcgc gactgttcgt
    27961 ttgaggttga agtgaaaaca ctaataagaa aattctcccg tacggccatc acggtcgtat
    28021 tagtcattct agccttcatc gcaattttta atgcctgggt ctattacacc gaatccccct
    28081 ggacgcgtga cgcgcgcttt agcgctgacg tcgttgcgat cgcgccggac gtttctggac
    28141 tcattaccca ggtgaatgtt catgataacc agctggtgaa aaaaggacag atactgttca
    28201 ccatcgacca gccgcgctat caaaaggcgc ttgaggaagc gcaagccgat gttgcttatt
    28261 atcaggtact ggcacaggag aaacgccagg aggccggacg tcgtaaccgt ctcggtgtgc
    28321 aggcgatgtc tcgcgaagag atcgaccagg ccaacaacgt actacaaacg gttctgcatc
    28381 agttagcgaa agcgcaggcg acccgcgatc tggcaaaact ggatcttgaa cgcacggtga
    28441 tccgcgcgcc agcagatggc tgggtgacca acctcaacgt ctataccggt gagtttatta
    28501 ctcgaggatc aacggcggtt gcgctggtga aacagaactc cttctatgta ctggcctata
    28561 tggaagaaac taagctggaa ggggggcgtc cggggtatcg tgcagagatc acgccgcttg
    28621 gcagtaacaa agtgctgaaa gggactgttg atagtgttgc cgcaggggtc accaacgcca
    28681 gcagcacgcg tgacgacaaa gggatggcga ctatagactc taatcttgaa tgggtgcgtc
    28741 ttgcgcaacg tgttccggtt cgtattcgtc tcgacaacca gcaagagaac atctggcctg
    28801 cgggcaccac tgctacagtg gtggtcactg gcaaacaaga tcgcgacgaa agccaggatt
    28861 cgttcttccg taaaatggcc catcgcctgc gtgagtttgg ttaatcacga tgggtatttt
    28921 ctccattgct aaccaacata ttcgctttgc ggtaaaactg gcgaccgcca ttgtactggc
    28981 gctgtttgtt ggctttcact tccagctgga aacgccacgc tgggcggtac tgacagcggc
    29041 gattgttgcc gccggtccgg cctttgctgc gggaggtgaa ccgtattctg gcgctattcg
    29101 ctatcgtggc tttttgcgca tcatcggcac atttattggc tgtattgccg gactggtgat
    29161 catcattgcg atgatccgcg caccattatt gatgattctg gtgtgctgta tctgggccgg
    29221 tttttgtacc tggatatcct cgctggtacg aatagaaaac tcgtacgcgt gggggctggc
    29281 cggttatacc gcgctgatca ttgtgatcac cattcagccg gaaccattgc ttacgccgca
    29341 gtttgccgtc gaacgttgta gcgagatcgt tatcggtatt gtgtgtgcga ttatggcgga
    29401 tttgctcttt tctccgcgat cgatcaaaca agaagtagat cgagagctgg aaagtttgct
    29461 ggtcgcgcaa tatcaattaa tgcaactctg tatcaagcat ggcgatggtg aagttgtcga
    29521 taaagcctgg ggcgacctgg tgcgacgcac cacggcacta caaggcatgc gcagcaacct
    29581 gaatatggaa tcttcccgct gggcgcgggc caatcgacgt ttaaaagcga tcaatacgct
    29641 atcgctgacg ctgattaccc aatcctgcga aacttatctt attcagaata cgcgcccgga
    29701 attgatcact gatactttcc gcgaattttt tgacacgccg gtagaaaccg cgcaggacgt
    29761 ccacaagcag ctcaaacgcc tgcggagagt tatcgcctgg accggggaac gggaaacgcc
    29821 tgtcaccatt tatagctggg tcgcggcggc aacgcgttat cagcttctca agcgcggcgt
    29881 tatcagtaac acaaaaatca acgccaccga agaagagatc ctgcaaggcg aaccggaagt
    29941 aaaagtagag tcagccgaac gtcatcatgc aatggttaac ttctggcgaa ccacactttc
    30001 ctgcattctg ggcacgcttt tctggctgtg gacgggctgg acttccggca gtggtgcaat
    30061 ggtgatgatt gcggtcgtga cgtcactggc aatgcgtttg ccgaatccac gcatggtggc
    30121 gatcgacttt atctatggga cgctggccgc gctgccgtta gggctgctct actttttggt
    30181 gattatccct aatacccaac agagcatgtt gctgctgtgt attagcctgg cagtgctggg
    30241 attcttcctc ggtatagaag tacagaaacg gcgactgggc tcgatggggg cactggccag
    30301 caccataaat attatcgtgc tggataaccc gatgactttc catttcagtc agtttctcga
    30361 cagcgcatta gggcaaatcg tcggctgtgt gctcgcgttc accgttattt tgctggtgcg
    30421 ggataaatcg cgcgacagga ctggacgtgt actgcttaat cagtttgttt ttgccgctgt
    30481 ttccgcgatg actaccaatg tggcacgtcg taaagagaac cacctcccgg cactttatca
    30541 gcagctgttt ttgctgatga ataagttccc aggggatttg ccgaaatttc gcctggcgct
    30601 gacgatgatt atcgcgcacc agcgcctgcg tgatgcgccg atcccggtta acgaggattt
    30661 atcggcgttt caccgacaaa tgcgccgcac agcagaccat gtgatatctg cccgtagcga
    30721 tgataaacgt cgtcggtact ttggccagtt gctggaagaa ctggaaatct accaggaaaa
    30781 gctacgcatc tggcaagcgc caccgcaggt gacggaaccg gttcatcggc tggcggggat
    30841 gctccataag tatcaacatg cgttgaccga tagttaagtc aaaaccgacg ccaaaagcgt
    30901 cggttttttc atggctatac ttagcgatgc acggcagaac tcgccgcgaa acgtgacggt
    30961 ggcaacagat gaatatttat acctttgatt ttgatgagat tgagagtcag gaggattttt
    31021 atcgtgactt tagccaaacc tttggtctgg ctaaagataa ggtacgcgat ctcgactcac
    31081 tatgggatgt gttaatgaac gatgtcctgc cgctaccact tgagattgaa tttgttcatc
    31141 tgggagagaa aacgcgtcgc cgttttggcg cgttaatatt gctgtttgat gaggcagagg
    31201 aagagctgga agggcatttg cgttttaatg ttcgtcatta gcgcaaaaaa aagcccccga
    31261 accgggggca atatcgtcgg acaagacgat gagggtttat ttgtacagtt cagccgtagc
    31321 gtgccaggtg tcaccgctac gagcttcagt aatctggtag gccgttgcgc ctttctcttc
    31381 cgcttttttg ttcagcattt cacgcatatc cattggcgaa gacgccacac cacttacgga
    31441 tacggtcccg attgcttcac gattttgtgc ttgtgcagta tcaatggagt cggcagcgaa
    31501 tgcaccgaaa gagagaacag aaagtacgct taatgcagct acagtggttt tgattttcat
    31561 gatttttacc tcgacataat cttttagctg ggcctttgtt tcgtgatcca tatcacaaaa
    31621 tcaagcatac actaattatt gaactaatta ataccacact aactgtttca gttgatacta
    31681 agtgttaaaa atgtgaattt taataacaaa aaagaattaa aaacacggtt atttattgtt
    31741 aattctagta ttttcaatta gataatgaat tttacataat gtgctttttt taccgtatgt
    31801 attcattgtg tgaatgatat gtcgcggtaa aacgcactat tcgttaaatt aatcgcaggg
    31861 gaaagcaggg ggttgagagg aataagcaac attttccccg ccgccagaag cgacggggca
    31921 gagattaaag ctcctggtcg aacagctcta aaatcgcttc gtacaggtct ttgacggtga
    31981 aaccgttagc aggggtggta aagatggtgt catcgccagc gatggtgccc agaatacctt
    32041 ctgctttgcc cagtgagtcc agcaggcgag caattaactg cgccgcgcca gggctggtat
    32101 gaatcacgac aactgcatcg ttgtagtcga tatccagcac cagattcttc aatggactgg
    32161 aggtggttgg tacacccagt tcagctggca ggcagtaaac catttccatt ttggcattgc
    32221 gtgtacgtac agcaccaaac ttggtcaaca tccgcgagac tttagactga ttaatattgt
    32281 caaagccttg ctcctgcaac gcggcgacga tttcgccctg ggagctaaat ttctcttctt
    32341 taagtaatgc tttaaatgct ttaactagtt cttcttgctt agccgagctt cgcataagtc
    32401 acccgatatg gtggttgata caacattatt gtgcatacag atgaattttt atgcaaacag
    32461 tcagccctga agaaggctga aataatgtta tgaaagagcg ggattttatc aaatttcgtt
    32521 attgagaaac atgcctgcgt cacggcatgc aaattctgtt taaaagtaaa ttaattgtta
    32581 tcaaattgat gttgttttgg ctgaacggta gggtatattg tcaccacctg ttggaatgtt
    32641 gcgctaatgc ataagcgact gttaattacg taagttaggt tcctgattac ggcaattaaa
    32701 tgcataaacg ctaaacttgc gtgactacac attcttgaga tgtggtcatt gtaaacggca
    32761 attttgtgga ttaaggtcgc ggcagcggag caacatatct tagtttatca atataataag
    32821 gagtttagga tgaaagtcgc agtcctcggc gctgctggcg gtattggcca ggcgcttgca
    32881 ctactgttaa aaacccaact gccttcaggt tcagaactct ctctgtatga tatcgctcca
    32941 gtgactcccg gtgtggctgt cgatctgagc catatcccta ctgctgtgaa aatcaaaggt
    33001 ttttctggtg aagatgcgac tccggcgctg gaaggcgcag atgtcgttct tatctctgca
    33061 ggtgtagcgc gtaaaccggg tatggatcgt tccgacctgt ttaacgttaa cgccggcatc
    33121 gtgaaaaacc tggtacagca agttgcgaaa acctgcccga aagcgtgcat tggtattatc
    33181 actaacccgg ttaacaccac agttgcgatt gctgctgaag tgctgaaaaa agccggtgtt
    33241 tatgacaaaa acaaactgtt cggcgttacc acgctggaca tcattcgttc caacactttt
    33301 gttgcggaac tgaaaggcaa acagccaggc gaagttgaag tgccggttat tggcggtcac
    33361 tctggtgtta ccattctgcc gctgctgtca caggttcctg gcgttagttt taccgagcag
    33421 gaagtggctg atctgaccaa acgcatccag aacgcgggta ctgaagtggt tgaagcgaaa
    33481 gccggtggcg ggtctgcaac cctgtctatg ggccaggcag ctgcacgttt tggtctgtct
    33541 ctggttcgtg cactgcaggg cgaacaaggc gttgtcgaat gtgcctacgt tgaaggcgat
    33601 ggtcagtacg cccgtttctt ctctcaaccg ctgctgctgg gtaaaaacgg cgtggaagag
    33661 cgtaaatcta tcggtaccct gagcgcattt gaacagaacg cgctggaagg tatgctggat
    33721 acactcaaga aagatatcgc cttgggcgaa gagttcgtta ataagtaatt gattagcgaa
    33781 taataaaaaa ccggagcaca gac
//
LOCUS       DAJTVJ010000036        33715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000036 DAJTVJ010000000
VERSION     DAJTVJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.036"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..84)
                     /locus_tag="OO448_001643"
     CDS             complement(<1..84)
                     /locus_tag="OO448_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6336878.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELG"
     gene            700..837
                     /locus_tag="OO448_001644"
     CDS             700..837
                     /locus_tag="OO448_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336879.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1437..2216
                     /gene="ipaJ"
                     /locus_tag="OO448_001645"
     CDS             1437..2216
                     /gene="ipaJ"
                     /locus_tag="OO448_001645"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6336880.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3162..4091)
                     /gene="virB"
                     /locus_tag="OO448_001646"
     CDS             complement(3162..4091)
                     /gene="virB"
                     /locus_tag="OO448_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6336881.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4308..4544)
                     /locus_tag="OO448_001647"
     CDS             complement(4308..4544)
                     /locus_tag="OO448_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6336882.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4548..6449)
                     /gene="ipaA"
                     /locus_tag="OO448_001648"
     CDS             complement(4548..6449)
                     /gene="ipaA"
                     /locus_tag="OO448_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6336883.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKESFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFFSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6458..7456)
                     /gene="ipaD"
                     /locus_tag="OO448_001649"
     CDS             complement(6458..7456)
                     /gene="ipaD"
                     /locus_tag="OO448_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6336884.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKKKYEDKPLYPATNTV
                     SQKEADKWLTELGGTIGKVSKKNGGYVVNINMTPIDNMLKSLNNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7507..8598)
                     /gene="ipaC"
                     /locus_tag="OO448_001650"
     CDS             complement(7507..8598)
                     /gene="ipaC"
                     /locus_tag="OO448_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6336885.1"
                     /translation="MEIQNTKSTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSTASQIAGNIRA"
     gene            complement(8618..10360)
                     /gene="ipaB"
                     /locus_tag="OO448_001651"
     CDS             complement(8618..10360)
                     /gene="ipaB"
                     /locus_tag="OO448_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6336886.1"
                     /translation="MHNVSTTTTGLSLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNSHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPDSPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10366..10833)
                     /gene="ipgC"
                     /locus_tag="OO448_001652"
     CDS             complement(10366..10833)
                     /gene="ipgC"
                     /locus_tag="OO448_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6336887.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10891..11517)
                     /gene="ipgB1"
                     /locus_tag="OO448_001653"
     CDS             complement(10891..11517)
                     /gene="ipgB1"
                     /locus_tag="OO448_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6336888.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSNMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11533..11922)
                     /gene="ipgA"
                     /locus_tag="OO448_001654"
     CDS             complement(11533..11922)
                     /gene="ipgA"
                     /locus_tag="OO448_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6336889.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11935..13419)
                     /gene="icsB"
                     /locus_tag="OO448_001655"
     CDS             complement(11935..13419)
                     /gene="icsB"
                     /locus_tag="OO448_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6336890.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13733..15349
                     /locus_tag="OO448_001656"
     CDS             13733..15349
                     /locus_tag="OO448_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6336891.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVNKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDSKIWNMVKGYSSFV"
     gene            15359..15721
                     /gene="ipgE"
                     /locus_tag="OO448_001657"
     CDS             15359..15721
                     /gene="ipgE"
                     /locus_tag="OO448_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6336892.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15721..16179
                     /gene="ipgF"
                     /locus_tag="OO448_001658"
     CDS             15721..16179
                     /gene="ipgF"
                     /locus_tag="OO448_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6336893.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16188..17303
                     /gene="mxiG"
                     /locus_tag="OO448_001659"
     CDS             16188..17303
                     /gene="mxiG"
                     /locus_tag="OO448_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6336894.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17311..17562
                     /gene="mxiH"
                     /locus_tag="OO448_001660"
     CDS             17311..17562
                     /gene="mxiH"
                     /locus_tag="OO448_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6336895.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17575..17868
                     /gene="mxiI"
                     /locus_tag="OO448_001661"
     CDS             17575..17868
                     /gene="mxiI"
                     /locus_tag="OO448_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6336896.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17874..18599
                     /gene="mxiJ"
                     /locus_tag="OO448_001662"
     CDS             17874..18599
                     /gene="mxiJ"
                     /locus_tag="OO448_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6336897.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18596..19123
                     /locus_tag="OO448_001663"
     CDS             18596..19123
                     /locus_tag="OO448_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6336898.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19065..19760
                     /gene="mxiN"
                     /locus_tag="OO448_001664"
     CDS             19065..19760
                     /gene="mxiN"
                     /locus_tag="OO448_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6336899.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19753..20160
                     /gene="mxiM"
                     /locus_tag="OO448_001665"
     CDS             19753..20160
                     /gene="mxiM"
                     /locus_tag="OO448_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6336900.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20144..20572
                     /gene="mxiM"
                     /locus_tag="OO448_001666"
     CDS             20144..20572
                     /gene="mxiM"
                     /locus_tag="OO448_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6336901.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20702..21334
                     /gene="mxiE"
                     /locus_tag="OO448_001667"
     CDS             20702..21334
                     /gene="mxiE"
                     /locus_tag="OO448_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6336902.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21321..23021
                     /gene="mxiD"
                     /locus_tag="OO448_001668"
     CDS             21321..23021
                     /gene="mxiD"
                     /locus_tag="OO448_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6336903.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFDRFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23040..24107
                     /gene="mxiC"
                     /locus_tag="OO448_001669"
     CDS             23040..24107
                     /gene="mxiC"
                     /locus_tag="OO448_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6336904.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24120..26180
                     /gene="mxiA"
                     /locus_tag="OO448_001670"
                     /gene_synonym="virH"
     CDS             24120..26180
                     /gene="mxiA"
                     /locus_tag="OO448_001670"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6336905.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26193..26594
                     /gene="spa15"
                     /locus_tag="OO448_001671"
                     /gene_synonym="spaK"
     CDS             26193..26594
                     /gene="spa15"
                     /locus_tag="OO448_001671"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6336906.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26598..27890
                     /gene="spaL"
                     /locus_tag="OO448_001672"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26598..27890
                     /gene="spaL"
                     /locus_tag="OO448_001672"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6336907.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKNRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27983..28321
                     /gene="spa13"
                     /locus_tag="OO448_001673"
                     /gene_synonym="spaM"
     CDS             27983..28321
                     /gene="spa13"
                     /locus_tag="OO448_001673"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6336908.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28308..29186
                     /gene="spa32"
                     /locus_tag="OO448_001674"
     CDS             28308..29186
                     /gene="spa32"
                     /locus_tag="OO448_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6336909.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29180..30061
                     /gene="spa33"
                     /locus_tag="OO448_001675"
                     /gene_synonym="spaO"
     CDS             29180..30061
                     /gene="spa33"
                     /locus_tag="OO448_001675"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6336910.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30066..30716
                     /gene="spa24"
                     /locus_tag="OO448_001676"
                     /gene_synonym="spaP"
     CDS             30066..30716
                     /gene="spa24"
                     /locus_tag="OO448_001676"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6336911.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30731..30991
                     /gene="spaQ"
                     /locus_tag="OO448_001677"
     CDS             30731..30991
                     /gene="spaQ"
                     /locus_tag="OO448_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6336912.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30991..31761
                     /gene="spa29"
                     /locus_tag="OO448_001678"
                     /gene_synonym="spaR"
     CDS             30991..31761
                     /gene="spa29"
                     /locus_tag="OO448_001678"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6336913.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31768..32796
                     /gene="spa40"
                     /locus_tag="OO448_001679"
                     /gene_synonym="spaS"
     CDS             31768..32796
                     /gene="spa40"
                     /locus_tag="OO448_001679"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6336914.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32809..33051
                     /locus_tag="OO448_001680"
     CDS             32809..33051
                     /locus_tag="OO448_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336915.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10515 a         4921 c         6748 g        11531 t
ORIGIN      
        1 ccccagttct ctggcaatcg cccggctact cattccctgc ttgtgcagga ttttaatttc
       61 cataactgtc tcaaaagtga ccataagctc tcctgaatca ggagagcaga ttaccccctg
      121 gatctgattt caggcgttgg gtgtggatca ctattgcacc gttcgtgaca cttgttgact
      181 gcctgtgcgg catctggtat caaggtggcg tgaagccggt tcaggttctc atgcggaacg
      241 ttggaaccta tatccccccg gtaccaaagg cgaaatgtaa gtgacaaaat gcaatgaaca
      301 cgttctccgt taactgagga gcatggtgat aaataaaggt aaatgcagaa ggtgtagagt
      361 ctcgcagatt tctgacatca tagagcctaa cattagatgc ataacgctac agattgtaga
      421 atctctacag gtgaaatata cactggttgg cgtttatgtc gcaaaacatg tcattcaggt
      481 tcatctcacc aataaggata tgagtgaagt ggaggataag tgagtctgct gtcagagttt
      541 tctggtgtat gtcagtaact gcgaaccatg tctgcttgat atgggagctt atggaggctc
      601 tctgtacaag gttagggagc taacaaagca gggataaaaa gtccgcctga tgcaggcacg
      661 gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga tgaatgttca ggctatcttt
      721 atcttgatgg tggttcagct gtttggtaaa gaaatcgctg taacaataga agaactgcag
      781 gcagtactgg tcccacatcg tttgctcagt caactggtga agttccgtag cgcgtaaatt
      841 aatgctttgc atgaaacgtt actggagtgg atgaaagaat acctgcatat atcattactg
      901 cgttggatta ccagtacaac agattgaatg aactggacat gttgatagac ggcattgaaa
      961 agcagcttat aagcatagtg cggcagagtt aattggagag cacttcgcct tctgagtatt
     1021 aagtaagcat ggtggtacct gcggatacta tctattcact gtgctcggtc ggcagcctta
     1081 ctgaccaagg agttggggga gtcgataaca aagttgaaaa aacagctctc aaccaaggtg
     1141 gttattgtaa caatagcaaa taagctggtg tgacctgaat cacaataacc gattatggac
     1201 gtaaatacga caaaaatatg tcagtatcag gcggtattaa ttgctgatgc tgatggacac
     1261 taactcttaa taaaagggtg gatgctgaaa ggttgttatg gtgacggacg tgatgacgac
     1321 ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg gaataaggac ccggtcagct
     1381 aattctcata gtagttcgca ttgctggatg caatactgtg ctgcaattac tcgtcgatgt
     1441 cggaacaacg gaagccttgc aaacggggat gtattcatac aggtgttatg ttatatgggg
     1501 ttttattgca gggggctata ccaagagaat atatgatatc tcatcagact gatgtgaggg
     1561 taaatgagaa tagagtaaac gaacaaggtt gttttttggc gaggaagcag atgtatgaca
     1621 attcttgtgg agctgccagt ttactctgtg ctgcaaagga attaggggtt gataaaatac
     1681 cacaatacaa agggtctatg tccgaaatga caaggaagag ttctcttgat ttggataatc
     1741 gttgtgagcg ggatttatac ttaataacta gtggtaatta taatccgaga atacacaaag
     1801 ataatatcgc ggatgccggt tattctatgc ctgataagat agtcatggct acgaggcttc
     1861 ttggattgaa tgcttatgtc gttgaagaaa gtaacatttt ttcgcaagta attagtttta
     1921 tttatcccga tgcaagggac ttattaattg gcatgggatg taatatagta catcaacgag
     1981 atgtgttatc ttctaaccaa agagttttgg aagctgttgc tgtatccttt attggagttc
     2041 ctgtcggcct ccactgggtg ttgtgtagac ctgatggaag ctatatggat ccagctgtag
     2101 gagaaaatta ttcttgtttt tcaactatgg aattaggtgc aagaagaagt aattctaact
     2161 ttataggata tactaaaata ggtatttcca tagttataac taatgaggct ttgtaaattg
     2221 tcgtttttta tctgtgaagg agcttgaata ttttgggggc ggggggcata acaaatgata
     2281 ttactgagta caaatttagc tatattgggc ggattagttg tgtgggaaga cgaaatgttt
     2341 ctcttgaaat aagtgaactc cctcaaggaa tgattgtgtg cttaaatatt taagtgctgg
     2401 taagtttgag aaggctgaca tattttgcag agcaaacagt gattgacttg gatgatattt
     2461 ttattttagt cactcgttgt aattggagta tgctttattt tatttgaaaa tagatgtatt
     2521 ttatacccgc aatttaaact gtaagcccta tgaggggtat ggatattata agttactctt
     2581 tgactaagca taacttttgg ggttagaaaa ataaatctct tgttaataat taatatgatt
     2641 ttaaaatcaa acttgtttat aattataaaa taatgtaatt cggtcatttt atgggaagat
     2701 taggagaata aaacagtaat tatatttgag aaatctgcta atatattctt aaagcttttt
     2761 tgatactgtg aacgaagtct gttaatggtg gattttttgt ttatcaccat gtaaaaaaat
     2821 tgagtagtaa aagcgttgac tgctaatcct cgctgttttt attgaaacaa ttaaaaatac
     2881 gctgaatatt ttcggccagt cactcatctt cacataaaat tttgaccatc atgccgcatc
     2941 ccttagttga caattcattt tcaatgatat gaatattact aatttcacca gataatgttc
     3001 tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt gcgtggggat
     3061 tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc aacgaagatt
     3121 tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga cgatagatgg
     3181 cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt gtttatttta
     3241 gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat gtaagtccgt
     3301 ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt tctgtttttg
     3361 cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa atttgtatca
     3421 aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc attagccttt
     3481 tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag ttctgacgcg
     3541 attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc acgagtgact
     3601 ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc tgatactttc
     3661 agaaaattaa gaccgatacc aagttctcgg atgctatgct cttttgctgt ttgtatatcg
     3721 tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag aacttcaaga
     3781 tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat ttcaattcta
     3841 ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt ttttatgata
     3901 tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt ttggttgacg
     3961 aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga aactttatta
     4021 ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa gtcgttgcac
     4081 aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa agttaaatgc
     4141 agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt aaaaattact
     4201 attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa tattcttgtg
     4261 gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta tattgatttt
     4321 gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc taatgcagat
     4381 tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat cagagaaaga
     4441 gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct gtacgctata
     4501 ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta atccttattg
     4561 atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt atatatatta
     4621 ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc tgtaagtaat
     4681 tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga tacttctttt
     4741 gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc tgttacgtag
     4801 tttattgtat ttgcatctgt aacttttaat ttgttgttta ttctgtgttc tgttgtggat
     4861 tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga tttagttcca
     4921 atgagagata taagattact tagggagttt gttacttttt ttgacgcttc aaatatcgtt
     4981 tcccgtgtta aatatgtgtc tccatttctt gagaaaaaat cagtctgaag tacttttgaa
     5041 tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag agaagcgcta
     5101 cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg gtggagaggg
     5161 ggggtggttg aagagttctg tatgttcaga ggcctgtgtc cccgagaaag agagttattt
     5221 gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc cgaattatct
     5281 ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg gacagtatct
     5341 gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg tggatttagt
     5401 aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga ctgtgcatca
     5461 ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac tctattatcg
     5521 taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt tttctcattg
     5581 gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag gccatcttgg
     5641 ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct caacagctca
     5701 ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa tcctttacta
     5761 tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc ctgtgcgtca
     5821 actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa ttcagcaaga
     5881 atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc caatgataaa
     5941 gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat gttttgtcgt
     6001 aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc ttgggataat
     6061 ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc cttttcgttt
     6121 tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa ttcgagaatc
     6181 tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac atcttctttc
     6241 cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc aaagctttct
     6301 ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat atcggatatt
     6361 ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata taagaatgtt
     6421 ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga aaaagtttat
     6481 ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta tcaaaaatac
     6541 tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt gtttcatctt
     6601 cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca acctcgccat
     6661 ttccacctag attatttaag ctttttaaca tattgtctat tggggtcatg tttatattga
     6721 caacatatcc cccgtttttt ttagatacct tgccgattgt tccacctaat tctgtaagcc
     6781 atttatctgc ttctttctga ctaacagtat tagttgctgg atatagcggt ttatcttcat
     6841 attttttctt gagttcttcc aatgcttttt taagcgagtt gacttgtaat ttcacggagt
     6901 ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca gcgctaaaat
     6961 cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga tactgttcat
     7021 taatatcatt gatggagttt gcaattttag cccacagttc tctatgagat atcatttgat
     7081 ctccatcaag ctcggcttct ttcggggctg aatgtaataa ttctcttgcg tctttattaa
     7141 ttggatattc gttcctggaa agaatatcca atagttgagc ggatttattt acatccatgc
     7201 taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac gttttttgtg
     7261 aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg tcgttgagca
     7321 tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa ttaactgttt
     7381 cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata ctattagtca
     7441 gagttgttat attcatggtt atttccttac aggaagagcc atatattatt atggctcttg
     7501 gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattcc ttgattgggt
     7561 gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt gggatgcttc
     7621 tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga ttagttgttc
     7681 ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac cagcaacggc
     7741 tgatgtgtcc attgttgcac ggccaatatt ttgtttttgc tgcgcagaaa gggtattgag
     7801 ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg gagaactaag
     7861 agaagtttta tgttcagttg acagggatat attatctggc gccagtttat ttttacctaa
     7921 aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt gtttatttaa
     7981 ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa ggttctttct
     8041 taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga tacccgttat
     8101 acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc cttgccgaac
     8161 aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt gagagcccaa
     8221 tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa ttaaagaaac
     8281 agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg tgtgactcat
     8341 ctctgaaaga tctttcatct ttttgtcagt aaggcgagca atgatttctg aatcatgctg
     8401 aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta atacgggatt
     8461 tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg atttttgtgt
     8521 ttcggaagaa ctttgagttt gttttgtgga tatatctgta tataaaatct gggttgattt
     8581 tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt tgttgcaaaa
     8641 ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct aatagatctg
     8701 caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat tttgacagtt
     8761 gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtagacgcg ctgttctgga
     8821 aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt tctcctaata
     8881 aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca tcacccgctg
     8941 caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc agttgagaag
     9001 aaaaattctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt attttgccaa
     9061 tattctctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga actactgctg
     9121 caaccaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt ttggcttttt
     9181 tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg agtttgatta
     9241 agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag gaattgccgg
     9301 tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct gccagtgcta
     9361 gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa agtgccccaa
     9421 gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt acttcagcag
     9481 catactcatc agattttctc tccatttcag tttttcttga ttcttggaga gactggaata
     9541 gagccagatc attttttaaa gattcttcat tattttttcc aactagttga ataaaggttg
     9601 ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt gatagctgtt
     9661 cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg agttgcattg
     9721 atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat gtcctgtctt
     9781 taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat tttttctttt
     9841 ctggtgaatc tgggtcgagt tcggataatc ttgtttgaat ttggttaatt ttattttcta
     9901 ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca tagtctctgg
     9961 ttagtccttc agtttcagat agaagagtat taattttatc ggagaattct aggttttttt
    10021 gctgtcttgc ctgttgctga gacttccaag cagtaatttt atttgttaat gcagttaaag
    10081 atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg gaacttgcat
    10141 ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag tgtgaattag
    10201 ttgtattaat atttttgtta gcagtaagat cagcaattgt aagagaaaat aatttgttag
    10261 ctgcatcatt tgcagcctgg atagtattgt ctccaagctc agtggaagcc aatattttgg
    10321 caagagacaa accagtggtt gtggtactta cattatgcat aataattact ccttgatatc
    10381 ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat tgctgtgttg
    10441 aattacgagt tcgaagcact ctttggcttt caagggggct ttcaaccgaa gctggcattg
    10501 tccagtatgg aataccggtg tatagtcatt ttttcctaat gcaaaagcga cagcataaag
    10561 gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc ccataatgta
    10621 gtctacattg taaaagtcgt atatacataa aaatctgaag aaaacttcag cttcctctat
    10681 tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca tatcatcagg
    10741 aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta ctgcagtaga
    10801 gatgctttca ttttcagtga tatttaaaga cataaggtct cctttttgtc tgctttactt
    10861 taataagtat aagatttaat ataaacagat ttaatttgta ttgctttgac ggtatacggc
    10921 ctttagaacc tctgcgctta tttctatttg taaaactttt gttgattcat taaatatttt
    10981 atcgtagacg gaactggata ctagatttgt aagatccttc atttgtgtgt taaacaaatc
    11041 catgttactg ctatagcgtt ttaaaatctt tgctctgagg ccagatgagg caatctcgtt
    11101 tagtgctgta tgactgatag atgtttgtgc tgatttttca tcaagtttta tatccgggaa
    11161 ttttttttca atagctgcaa atatgttttt tttgttatct gaggtcaaaa caactccatt
    11221 gtgtatggcc atttcatcta tacttctgtt tatcattgaa gagacataag aggttcgctc
    11281 ttggctcatc caacaaaata gttgattccc cgaattacta tcttttattt tgtctatgac
    11341 gttgtccctg attttattta tattcaccgg gcatccaaaa ttcttctgag gaaagcgttg
    11401 ttttaagtta aaaacagata atattttctt tacaagctta ttgtaactta acgaattaaa
    11461 tgatgggcga ggaatcgcga attcaacctg tggaagtatt ttgtttagaa tttgcatgat
    11521 accccctata tgttagttca cttctgaagt gatgtttgca atggttagtt taatacaaaa
    11581 taaaaattca tacaaagatt caatgagttc atcaggtgaa agatattttt tgtagtgttt
    11641 cttggtcaaa acacattgat gagtatcagg gagaagacag atagaagaac cataatctct
    11701 actcaaagag tagttaagga tgagtatgtg ttctggatta ataatatttt ctgtaaaaag
    11761 ttcatgttca ttaagaagtc cggtcaaaaa gatgtattca tcattatctg ttatacaaac
    11821 aggtatttgt tcttcgagca atataaaggc ttgattttta tcgttaaaat caagctttgc
    11881 acctgttatt tcataaagtt tatcatatag tttgcgacac atttctatgg ccttctatat
    11941 attagaatga gagttattca ataaaagctt attgacctgt aataagtcgt gaatgtaaag
    12001 tttggttcca agatctggcg atttaagaga attgtaataa tcaatagttt ttattgactt
    12061 ttcgataatt tcttcttttt tatctgttgc tccggttttt aattcgttta atacacgaga
    12121 catattgctg ggtgctgatt ttactaagta tgagtggcaa gcgttgatga ccgcatcaaa
    12181 gtcttctgaa gatacatttt cagtaggatg tttgtaaagt gaaattgata aaggcttgaa
    12241 tgtttttggg gtgtggataa ggttaatttt attgaaactt ttaagatagc tacgtcgtaa
    12301 ttgagtcaat ctggaaagat caaaatcaga ttcaatatac ttcattatct cgttataaga
    12361 ctgattgaga ctttcaatgc gttgcctaac tttatttgca tacgcgtata cattgttagg
    12421 tgtagcaacc aatttaacgt cagggagtgg aaagtatatt tcagaatttc ctgctttaag
    12481 aacattaagt gccataccag cacagttttc agatttactg attaatttat aatttgccaa
    12541 ttgtctgaaa gcatctgctt tcttttggca tataaatttt gacatgtttg tttcatcaag
    12601 gccaaaaagg ttatagctga ttttaccatc ctttgtttta ttatcacctg ataatggaat
    12661 gtatacttta tcagcactga ccccccaata accatagggg tctttttttt gattagctct
    12721 gggaaaaaat atttttttgt tgttattttg atcttgtttt gaatttaata agtttctaac
    12781 ctcttggcct gcatcaagtc tttcggctgt ttggtctgaa atcatattta gtttgtcttg
    12841 tttataggta tcgacactgt aacctgagcg tttttcaaaa aggtagttta tttctgtatc
    12901 ttttgtaaca ttcccgccgg gataccagct gagataggta tgagttggtt cctgtagtaa
    12961 attgtttttt atactgatac tggtatgccc taaatgggtt atgcctctat ttccccagca
    13021 acgaaaaact aaatgtcttt caagattttt ggctgtaccg atgccatgaa aacgctctgc
    13081 ggtattcaac attaatttga tgtctttacc tgtaaaagaa tgttgaattg aatttttgac
    13141 tgcttcatag gcggcaaacg cactttctgc agatttttcg ctagctaatg tcttgaggaa
    13201 aagcaataag gatttttgat tcgctagtcg ataatttttt agttgttcat ttaatatctg
    13261 tgaatttatc tttgcattaa tagtgcttaa tgttctgaaa aagagatcct ttaggttgaa
    13321 tttctgtgct accaatatag gtccatgctc tgtatcttca acgcgtatag gcccttttgt
    13381 attgcttgcg tcaatgaaat tgctaatttt gaggatcata ctttattaac tctccattac
    13441 gtggtgattg tggggatgta aaatgtattt gatatataat atattcaatt aataaattta
    13501 gaaacttgag cctgttaaca taatcaaatt ttcttttgtt gtacataata tgtacctcgt
    13561 gagcatatgt agtgctcgtt tcatcatgaa atcccacaag ttaaagtgtc tgatatatca
    13621 ggctcggagt gttatagaaa aagagagaac cctgttgaat aagtatcttt tttgttgtaa
    13681 aacgaggtac aaaactgcaa tcaatgcccc atttatgact gaggataatt aaatgcacat
    13741 aactaatttg ggattgcatc aggtttcatt tcaaagcgga gattcctata aaggcgcaga
    13801 agaaaccgga aagcacaaag gtgtaagcgt gatttcatat caaagagtta aaaatggaga
    13861 aagaaataag gggatcgaag cgcttaatcg attatattta cagaaccaaa caagcttaac
    13921 aggaaaaagc cttttgtttg cgcgtgacag agctgaagtt ttttacgagg caataaagtt
    13981 ggcaggtggt gatacgtcaa aaattaaagc catgatggaa cgattagata cctataaact
    14041 tggtgaagtt aataaaagac atattaatga gcttaataaa gtaataagtg aagagataag
    14101 agcacagcta ggcattaaaa ataaaaagga attgcaaacg aaaataaaac aaatatttac
    14161 tgattattta aataataaga attggggacc ggtcaacaag aatattagtc atcatgggaa
    14221 aaattatggt tttcaattaa cacctgcctc tcatatgaag atagggaata aaaatatatt
    14281 tgtcaaagag tataatggaa aaggaatttg ttgtgcttct acgagagaga gtgatcatat
    14341 cgcgaatatg tggctttcaa aagtggtgga tgacgagggg aaggaaattt tttcgggtat
    14401 cagacatgga gttatatcag cctatggatt gaaaaaaaac tcatctgaaa gagctgttgc
    14461 tgctcgtaat aaagctgagg agttagtaag cgcagcatta tatagcagac cagagttatt
    14521 atcacaggct ttgtctggta aaacagtaga tttaaagatt gtttcgactt ctcttctgac
    14581 gccgaccagt ttaaccgggg gagaggagag tatgttaaaa gatcaggtga acgcattaaa
    14641 agggttaaac tctaaaaggg gggagccaac gaaattatta attcggaata gtgatggtct
    14701 tctgaaagaa gtaagcgtta atctaaaagt ggtgacattt aactttggtg taaatgaatt
    14761 agcgctaaaa atgggcttag gctggaggaa tgttgacaag cttaatgatg aatcaatatg
    14821 ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt ggaggttggg ctgcagaagc
    14881 aatagaaaaa aatccaccat gtaagaatga tgtgatatat ctggctaacc agataaaaga
    14941 gattataaac aagaagttac aaaaaaatga taatggagag ccgtataaat tgtcacaaag
    15001 aatgactcta ttggcttata ctataggtgc tgtaccttgc tggaattgta agagtgggaa
    15061 ggacagaaca ggtatgcagg atgctgaaat taaaagagaa ataataagaa aacatgaaac
    15121 aggtcagttt tctcaattaa atagtaaatt atcctcagaa gagaaaagat tattttctac
    15181 tattctaatg aatagtggta atatggaaat ccaagagatg aatactggtg tgcccggaaa
    15241 taaagtcatg aaaaaattgc cgctatcttc gttagagcta tcttattctg aaagaatagg
    15301 ggactcaaaa atatggaata tggtgaaagg gtattcgtca tttgtataag aggaatatat
    15361 ggaagattta gcagatgtta tttgccgagc cttgggtatc cctttaattg atatcgatga
    15421 tcaagcaatc atgcttgatg atgatgtgct aatatatatt gaaaaagaag gagattcaat
    15481 aaatttatta tgcccttttt gtgcattgcc tgaaaatata aatgatctta tatatgcgtt
    15541 gagcctaaat tactcagaaa agatatgctt agctactgat gatgaaggcg gaaatctaat
    15601 tgcgcgatta gatcttaccg ggataaatga attcgaggat gtatatgtta atacagaata
    15661 ttatatttcg cgtgtgcgtt ggctgaagga tgaatttgcg cgaagaatga aggggtatta
    15721 atgtcccgtt tcgtatttat tttgctgtgt tttatcccat atttagggag agctgattgt
    15781 tgggataagg ctggtgaaag gtacaatatt ccatcaagtt tgttaaaagc gattgcggaa
    15841 aaagaatccg gatttaataa atttgcagtc aatgttaata acaatggaag taaagattat
    15901 ggtataatgc aaataaatga ctttcattct aaaagactta gagaaatggg atattctgag
    15961 gaaatgctaa ttagtcatcc atgtctttct gtacattatg cagcaaagtt attaaatgag
    16021 tttatgatga tgtatggaag agggtgggag gctgttggag cctataatgc aggaacgtct
    16081 ccgaaaaaga aaaaagaaag attaaagtac gcagaggata tctatagacg ctatttgcgg
    16141 atagcagcag agagcaagca gaataatcga aggatataag aggatttatg tctgaggcaa
    16201 agaactcaaa tcttgcacca ttcaggttat tagtgaaact cacaaacggt gtcggggatg
    16261 agtttccgtt atattatggg aataacttaa ttgttctcgg acggacgata gaaacacttg
    16321 agtttggaaa cgacaatttc ccagaaaata ttattcctgt aacagatagt aaatcggatg
    16381 gtataattta tttgaccata agcaaagata atatctgtca atttagtgat gaaaaaggtg
    16441 agcaaattga tatcaattca caatttaatt catttgaata tgacgggatt tcttttcacc
    16501 ttaaaaatat gagggaggat aaatcgagag gccatatact aaacggaatg tataagaacc
    16561 actcggtgtt ttttttcttt gctgttattg ttgtgttaat tataattttt tccctctctt
    16621 taaagaaaga tgaagtaaaa gaaattgctg aaattataga tgataagaga tatggaatag
    16681 tcaacactgg acagtgcaat tatattcttg ccgaaacaca aaatgatgcg gtatgggcat
    16741 ctgtagcttt aaataaaacc ggatttacca aatgcagata tattctggtg tcaaacaaag
    16801 aaataaatag aattcaacaa tatatcaatc aacgattccc atttatcaat ttgtatgtgt
    16861 tgaatttagt gagtgataaa gcggaattac tggtgttttt aagtaaagaa agaaatagtt
    16921 caaaagatac cgaacttgat aaattgaaaa atgctctgat tgttgaattt ccgtatatca
    16981 aaaatattaa attcaactat cttagcgatc ataatgcaag gggggatgct aaaggtattt
    17041 tcacaaaggt aaatgtccag tataaagaga tttgtgaaaa taataaagtt acatattctg
    17101 tcagagaaga actgacagat gaaaagcttg agctcattaa tagactgatt tctgaacata
    17161 aaaatatata tggagatcag tatattgagt tttctgtact tttgatagat gatgatttta
    17221 aaggtaaatc atatcttaac agcaaagaca gttatgtaat gttgaatgat aaacactggt
    17281 tttttttaga taaaaataag tgaggataaa atgagtgtta cagtaccgaa tgatgattgg
    17341 acactgagtt cattatctga aacttttgat gatggaactc aaacattaca aggtgaacta
    17401 acattggcac tagataaatt agctaaaaat ccttcgaatc cacagttgct ggctgaatac
    17461 caaagtaaat tatctgaata tacattatat aggaacgcgc aatccaatac agtgaaagtg
    17521 attaaggatg ttgatgctgc aattattcaa aacttcagat aatagggagc attcatgaat
    17581 tacatttatc cagtcaatca ggttgatatt atcaaagcca gtgattttca atctcaagag
    17641 atatcaagtc tggaagatgt cgtgtcggct aaatatagtg atattaagat ggatacagat
    17701 attcaagtat cacaaataat ggagatggta agcaatccag aatcattaaa cccagaatct
    17761 ttggccaagt tacagacgac gctctcaaat tattcaatag gagtatcatt agctggcacg
    17821 ttagcaagaa aaacagtttc ggctgttgaa actttattaa agtcttaatt tatatgatta
    17881 ggtataaagg ttttatttta ttcttgttgc tgatgttgat tggatgtgag caacgtgaag
    17941 agttaatttc taatttatct caaagacagg caaatgaaat aatatctgtg ctagaacgcc
    18001 ataatattac tgctagaaaa gttgatggag gtaaacaggg gatctcggta caagtcgaaa
    18061 aggggacatt tgcatcggca gttgatttga tgcgcatgta cgatttgcca aatccggaga
    18121 gagttgatat ctcacaaatg tttcctacag attcattagt gtcttctcca agagctgaaa
    18181 aggcccgttt atatagtgct attgagcaac ggctggaaca gtctttagtt tctattggtg
    18241 gtgttatttc ggcaaaaata catgttagct atgatcttga agaaaaaaat atatcttcaa
    18301 aaccgatgca tatatcagta atcgctatat atgactcacc gaaagagtct gaactattag
    18361 ttagtaatat taagcgattt ttgaaaaaca ccttttctga tgttaagtat gaaaatatat
    18421 ctgtcatatt aactccgaaa gaagaatatg tttatacaaa tgtacaacct gttaaggaaa
    18481 ttaaatcgga atttttaaca aatgaagtaa tatatttatt tctcgggatg gctgtactag
    18541 ttgtcattct tttggtatgg gcattcaaaa cagggtggtt caagagaaac aaaatatgat
    18601 aagaatggat ggaatttata aaaaatatct ttcaataatt tttgatccag cgttctatat
    18661 aaatagaaat cggttgaatt tgccttctga actgttagaa aatggcgtaa tcagaagtga
    18721 gattaataat ctcataatta ataaatatga tctaaattgc gatattgaac ctttaagcgg
    18781 ggtaaccgct atgtttgttg ccaactggaa tttacttcca gctgttgctt attttatcgg
    18841 tagtcaagag tctcggttaa ttaatcatag tgagatggtt attagttatt atgggggcaa
    18901 aatcagtaag caaggggagg ccgctattcg aagtggtttt tggcatctta ttgcgtggaa
    18961 ggaaaatata tctgttggta tatatgaaag aataaactta ttatttaatc caattgcttt
    19021 ggagggaaat tatacgcctg tagaaagaaa tttgtcccga ttaaatgaag gtatgcaata
    19081 tgcaaaaagg cactttaccg gtatccagac atcatgccta tgatggtgtt gttataaaga
    19141 gaattgaaaa ggaattatgt aaaaccataa aagatagaga cactgagagt aagaagaaag
    19201 caatttgtgt tataaaggat gctacgaaaa aagctgaatc actgcgtata gatgctgtat
    19261 gtgatggtta tcaaattggt atacagactg cttttgagca tattattgac tatatttgtg
    19321 aatggaagtt aaagcaaaat gaaaacagaa gaaatattga ggactatata actagtttgc
    19381 tttcagaaaa tttacatgat gagaggatca ttagcacttt attggagcag tggcttagtt
    19441 cgttgcgtaa tacagtaaca gaattaaaag tggttttgcc caagtgtaat ttagcactga
    19501 ggaaaaagtt agaacttgat ctccataaat atcgctcaga tgtcaagatt attttaaaat
    19561 attctgaagg gaataattat atattctgta gtggtaatca agtagtggag tttagtcctc
    19621 aggatgtaat atcaggtgtt aaaatagaac ttgcggaaaa attaacaaaa aatgataaaa
    19681 aatatttcaa agagttagca cataagaaac tgagacaaat tgcagaagac ttgttaaaag
    19741 agaatcctgt taatgattaa tcaaataaat gcaagcaatg ctctgcagca aagattaaat
    19801 agtgaagaat ttgttaattt aaatgagaga ttatcatctt cgcaatcatt tgatgaggat
    19861 ataatctatg aaataatgca atatttctca cagagtgaat taaattccat agataatgat
    19921 gaactgcaca ataagattga acaattattc aattcacgtt ttccttatct tacagctgca
    19981 caaaaaagtt cacttcttaa taaactcata gatgcaaatc aatatgtcga tttgcatgag
    20041 ggattttatg catcattgtc catatataac aatattgatt tctatattaa gactacaacc
    20101 ttcgattcat taattagtgt ctttgaagca gggagagagg cagatgattc gacatggtag
    20161 taataagttg aaaatattta ttttaagtat attgctatta acactgagtg ggtgtgcttt
    20221 aaagtcatca tctaattctg aaaaagaatg gcatattgtt cctgtaagta aggattattt
    20281 ttctattcca aatgatttat tatggtcgtt taatacaacc aataaaagta taaatgttta
    20341 ctctaaatgt attagtggta aggcggttta tagttttaat gcaggtaaat tcatgggcaa
    20401 ctttaatgtt aaggaagtag atgggtgctt catggatgca caaaagatag ctatagataa
    20461 actattttct atgctgaaag acggggttgt tttaaaaggt aataagataa atgataccat
    20521 ccttatagag aaggatgggg aagttaaatt aaaattaatt cgagggatat aattgtattg
    20581 tgagtaaata taaaggtcta aatacaagta atatgtttta catttactct agtggacatg
    20641 aaccagttaa cgttgagctt gtaaaagata aagaacgtaa cataatcgag ctggctccag
    20701 catggaaggg tttttttttg tccgaaatca aaatataaaa ttcagtgata atgtgaacta
    20761 tcattatcgc tttaacatta atagttgtgc aaaatttctt gctttttggg actatttcag
    20821 tggtgctcta gtagagcata gccatgctga aaaatgtatt catttctatc atgagaatga
    20881 tcttcgggat agttgtaata ccgaatcgat gcttgataag ttaatgttaa gattcatctt
    20941 ttcatctgac cagaatgtaa gtaatgcact ggctatgata cgaatgactg agtcatatca
    21001 tttggttctt tatcttctga gaactattga gaaagaaaag gaggtaagaa taaagagtct
    21061 gacagagcat tatggtgtgt cggaggctta ttttagatct ttatgtagaa aagctttagg
    21121 ggcaaaagtc aaagaacaac tgaacacttg gcggttagta aatggtttat tagatgtatt
    21181 tttgcacaat caaacaatca cttctgctgc catgaataat gggtatgcat caacatccca
    21241 tttttcaaat gagataaaaa cacgactagg cttttctgca agagaattat cgaatattac
    21301 ttttttagtg aaaaaaataa atgaaaaaat ttaatattaa atctttgact ctcttgattg
    21361 tattgttacc attgatagtt aatgctaata atattgactc tcatttatta gaacaaaatg
    21421 acatagcgaa atatgttgct caaagtgata cggtaggatc tttttttgac cgtttttctg
    21481 cgttattaaa ttatcctatt gttgttagca aacaagctgc aaaaaagaga atatctgggg
    21541 agtttgattt atctaatcca gaagaaatgc ttgaaaaact gacattactt gtgggactca
    21601 tatggtataa agatggaaat gcattataca tatatgactc tggagaatta ataagcaaag
    21661 ttattttgct ggagaatata tcacttaatt atcttattca gtatctgaag gatgctaacc
    21721 tttatgatca cagatatcct ataagaggaa atattagtga taaaacattc tatatatctg
    21781 gtcctccggc attggttgaa ctggtagcta atacagcaac tttgcttgat aagcaggttt
    21841 cttctattgg gacagataaa gttaattttg gggttataaa attaaaaaat acatttgtta
    21901 gtgacagaac ttataatatg agaggggagg atattgtcat cccaggggtg gctactgttg
    21961 tagaacgttt attaaataat ggtaaggctc tgagtaacag acaagcccaa aatgatccga
    22021 tgccaccctt taatattaca cagaaagtat ctgaggatag taatgacttt tcatttagtt
    22081 cagttaccaa tagttctata ttagaggatg tatcacttat tgcatatcca gaaaccaatt
    22141 ctatattagt caaaggtaat gatcagcaaa tacagattat tcgcgatatt atcactcagt
    22201 tagatgtagc caaacgacat atagaattat cattatggat aatcgatatt gataaatctg
    22261 aattaaataa ccttggtgtt aattggcaag gaacggcttc atttggtgat tcgtttggtg
    22321 cgtcatttaa tatgagctca tcagcatcga taagcacttt ggacggcaat aagtttattg
    22381 cttcagttat ggcattaaat cagaaaaaga aagcaaatgt agtttcaagg ccggtgattt
    22441 tgacacaaga gaatattccg gctatatttg ataataacag gactttttat gtgtcattag
    22501 ttggtgagcg caattcttcg ttggagcatg ttacgtatgg aacattgata aatgttatac
    22561 ctcgcttttc atctcgggga caaattgaga tgtctttaac gatagaggat ggaacaggaa
    22621 atagtcagtc aaattacaat tataataatg aaaatacatc tgtccttcca gaagttggaa
    22681 ggaccaaaat cagtacaata gcgagagttc cacaaggcaa aagtttgttg ataggtggtt
    22741 atacgcatga aacgaactct aacgaaatag tatctattcc ttttctctcc tctattcctg
    22801 taatcggtaa tgtgttcaag tataaaacaa gtaatatcag taatattgta agggtttttc
    22861 tgattcaacc ccgtgagata aaggagtcaa gttattataa tactgcagaa tataaaagcc
    22921 tcatatctga aagagaaata caaaagacta cacaaataat cccatctgaa actactttgc
    22981 tggaagacga aaaatcattg gtttcatact taaattacta actataaagt aggtgatgta
    23041 tgcttgatgt taaaaataca ggagttttta gctctgcatt cattgataag ttgaatgcaa
    23101 tgacaaattc agatgatgga gatgagactg ctgatgcaga gcttgattct ggcttggcta
    23161 atagcaagta tattgactca tctgatgaga tggcttccgc tctttcgtca tttataaaca
    23221 gaagagacct tgagaaactg aaaggaacaa atagtgatag tcaggaacgt attttagatg
    23281 gggaagaaga tgaaattaat cacaagattt ttgatttaaa gagaacgtta aaagataacc
    23341 ttcctctaga tcgggatttt atagacagac taaagagata ttttaaagat ccaagtgatc
    23401 aagtcttagc attaagggaa cttttgaatg aaaaagatct tactgctgaa caagtcgaat
    23461 cattaactaa aattattaat gagataatat caggtagtga aaaaagtgtt aatgctggaa
    23521 taaattcagc tatacaggct aaattatttg gcaacaaaat gaaacttgaa ccacagcttt
    23581 tgcgtgcatg ttatcgtggt tttatcatgg ggaacatatc aacaacagat cagtatatag
    23641 aatggcttgg taattttggt tttaatcaca gacatacaat tgtgaatttt gtagagcagt
    23701 cactgattgt agacatggat tctgagaaac cgagctgtaa tgcttatgag tttggttttg
    23761 tgttatctaa attaattgca attaagatga ttagaacttc agacgtaatt tttatgaaga
    23821 aactggaatc ctcaagcttg ctaaaagatg gcagtttaag tgcagagcag ctattgctaa
    23881 ctttattata tatttttcaa tatccaagtg aaagtgagca aattcttact tctgttatag
    23941 aagtatcacg agccagtcat gaggattctg tagtgtatca aacatatcta tcttctgtta
    24001 atgaaagtcc tcatgatata tttaaaagtg aaagtgaaag agaaattgcg atcaatattc
    24061 tacgagagct tgtcacaagt gcatacaaga aagagctttc tagataacag gagatgaaag
    24121 tgatccagtc ttttcttaag caagtaagta ctaagcctga attaattata ttagtgctta
    24181 tggttatgat cattgctatg ttgatcatac cattacctac ataccttgtt gattttttaa
    24241 tagggcttaa tattgtactt gctattctcg tttttatggg atcattttat attgaaagaa
    24301 tattgagttt ttctacattc ccttcggttt tgttgataac gacattattt cgtctcgctt
    24361 tatcaataag tactagtcga ctaattttag ttgatgcgga tgcgggtaaa attattacta
    24421 catttggcca gtttgtcatt ggtgatagtc tggctgtagg ttttgttatt ttttcaatag
    24481 taactgttgt gcagttcatt gttataacaa aaggatctga aagggttgcg gaggtagctg
    24541 ctcgcttttc tcttgacggt atgccgggga aacaaatgag cattgatgcg gatttaaagg
    24601 ctggaattat tgatgccgca ggagctaaag aaagacgcag tattctggaa cgtgagagtc
    24661 aattgtatgg ttcatttgat ggagcaatga agttcattaa aggtgatgca attgctggca
    24721 tcattattat ttttgtcaat ttaataggag gtatttctgt tggtatgagt caacatggaa
    24781 tgtccctatc tggtgcatta tctacttata ccatactaac tattggtgac ggattggtct
    24841 ctcagattcc tgctctgtta atttctataa gtgcaggatt tatcgttaca agagttaatg
    24901 gtgatagcga taatatggga cgtaacataa tgtcccagat atttgggaat ccttttgttc
    24961 tcattgttac atcagctctt gccttggcga tagggatgtt gccaggtttt ccgttttttg
    25021 tttttttcct gatagcagtt actttgacgg ctttattcta ttataaaaag gtcgtagaaa
    25081 aagaaaaaag tctgtctgag tctgattcta gtggctatac tggtacattt gatattgata
    25141 atagccatga ctcatctttg gcaatgatag aaaatctgga tgctattagt tcagaaaccg
    25201 ttcctttaat tttattattt gccgaaaata agataaatgc aaatgatatg gaaggtctta
    25261 ttgaaaggat aagaagtcag ttctttattg attatggtgt caggcttcca actattttat
    25321 atagaacaag caatgagctt aaggtcgatg atattgtttt gttaataaat gaggtgcgtg
    25381 ccgatagttt taatatatat tttgacaaag tgtgtattac agatgaaaat ggagatatag
    25441 atgctctagg tattcctgtt gtttcaactt catataatga acgtgttatt tcttgggtag
    25501 acgtttcata tacagaaaat ctaactaata ttgatgctaa aattaaaagt gctcaagatg
    25561 aattttatca ccagttgtca caagctttat taaacaacat aaatgagatt tttggtatac
    25621 aagaaacaaa aaatatgtta gatcagtttg aaaatcggta tcctgatcta ttaaaggaag
    25681 tcttccgaca tgtgactata cagagaattt ctgaggtatt acaaagattg cttggagaaa
    25741 atatttctgt tcgcaattta aaacttatta tggagtcttt ggcgctttgg gctcccagag
    25801 aaaaagatgt cataacatta gttgaacatg tccgtgcatc actttctagg tatatttgta
    25861 gtaaaatagc tgtttctggt gagattaaag ttgtgatgct ttccggatat attgaggatg
    25921 caataagaaa agggataagg caaacctctg gtggctcttt cttgaatatg gatatagagg
    25981 tttcggatga ggtaatggaa actttagcac atgctttgag agaattgaga aatgcaaaaa
    26041 aaaatttcgt tcttttggta tcagtagata tacgtaggtt tgttaaaaga cttatagata
    26101 acagatttaa gagtatactc gttatatcgt atgctgagat tgatgaagca tataccatta
    26161 atgtattaaa gactatttag tgaggtttaa atatgagtaa cattaattta gttcaattag
    26221 ttagagatag tcttttcacg attggttgtc ctccatcaat tattacagat ttagacagcc
    26281 attcagcgat taccatttct ttggatagta tgcctgctat taatatagct cttgtcaatg
    26341 agcaggttat gttatgggct aattttgatg caccaagtga tgtaaaactt cagtcatctg
    26401 cttataacat attaaattta atgttaatga actttagtta tagcattaat gagctggtag
    26461 agcttcatcg ttctgatgag tacttacagt taagagttgt tattaaagat gactacgttc
    26521 acgatgggat tgtttttgca gagattctgc atgagtttta tcagaggatg gaaatcttaa
    26581 atgaggtctt ataatcaatg agctatacaa aattgctcac tcaattatct tttcctaata
    26641 gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt gagatttgta
    26701 acattcaggc tggaattgaa agtaatgaaa ttgttgcaag agctcaggtt gtaggatttc
    26761 atgatgaaaa aacaatatta agcttgattg gaaattctcg tggactttca cggcaaacgt
    26821 tgattaagcc cactgcccag tttcttcata cgcaagtcgg ccgtggatta ttgggagcag
    26881 tagtcaatcc tttaggggag gttactgata agtttgctgt tacggataac agtgaaattc
    26941 tttatcgacc tgtagataat gctcctccgc tatatagtga aagggctgca attgagaagc
    27001 cttttttaac aggtattaag gttattgatt ctttactcac gtgtggtgaa ggacagcgaa
    27061 tggggatttt tgcgtcagct ggttgtggca aaacttttct catgaatatg ctcattgaac
    27121 atagtggtgc tgatatatat gttattgggt taattggtga gcgaggtcga gaggttacag
    27181 aaacggttga ttatttgaaa aactctgaga aaaaaaacag gtgtgtttta gtatatgcaa
    27241 cttcggatta ctcttcggtt gatcgttgta atgctgcata tatagccact gctatagccg
    27301 aattttttag gactgaagga cataaagtag cgctttttat tgattcatta acaaggtatg
    27361 ccagagcatt acgtgatgtg gccttagccg ctggagaatc acctgccaga agaggctatc
    27421 cggtttcggt ttttgatagc ttacccagac ttcttgaaag gccaggaaag ttaaaggcag
    27481 gtggctctat tactgcattt tacactgttc ttttggagga tgatgatttt gctgatccat
    27541 tagctgaaga ggtaagatcc attttagatg gacatatata tttgagcaga aatctagccc
    27601 aaaaaggaca atttcctgca attgattcct taaaaagtat aagcagggta tttacacagg
    27661 ttgttgatga aaaacatcgt attatggccg ctgcatttcg ggagctactt tctgaaatag
    27721 aagagctaag gacaattatt gactttggtg aatacaaacc gggggagaat gcctctcagg
    27781 ataaaatcta caacaaaata tctgttgttg agagttttct gaagcaagat tatcgactcg
    27841 gttttactta tgagcagaca atggagctta ttggtgaaac aattagataa ggttttaaaa
    27901 atcaaagata aatatcaacg gtctgttaaa ttaattgaag cacacatatt aacactaaaa
    27961 aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat acttcctgca
    28021 actggagaat gacttagaac ctgtaggcgc tcagagtgtt agtcagcttt tcaatacaag
    28081 acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac gaattcttct
    28141 aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt ctaaaagaaa
    28201 actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa gaaataactt
    28261 tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg gcattagata
    28321 atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa ctatcttcaa
    28381 tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt cctgagtact
    28441 ctttaattgc atctaactat tttactattg ataagcactt tgaacataag catgataaag
    28501 gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga gcgacatatt
    28561 ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat caagatataa
    28621 aagcaaggga aaaaataaat atcggcgata tgagggggat cttttcatat aataagagtg
    28681 gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct gataatctgc
    28741 tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg tctattgata
    28801 agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca tttgagctgc
    28861 ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa gaaaaatcaa
    28921 tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac cactttaaac
    28981 agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt gttctaaaac
    29041 cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat gctgagggta
    29101 attatagatt tgatagcagt caacataata aggggaataa aaataatagt acaggatata
    29161 atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg aaaaactaca
    29221 gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca aaaataaatt
    29281 tacaggcagt gaaagtttag tcactcttac ttccgtgtgt ggggattggg taattcgtat
    29341 tgatacatta tcttttttga aaaaaaaata cgaggtattt tcaggatttt ctacacaaga
    29401 atctttactg catttatcaa aatgtgtctt tatagagtcg tcatctgtat tttcgattcc
    29461 agaactgtct gataagatta ctttccggat cacgaatgaa atccagtatg caactactgg
    29521 gagtcattta tgctgttttt cctcttcttt aggtattatt tattttgaca agatgccggt
    29581 attacgtaat caagtttctc ttgacttatt gcatcatctt ttagagtttt gcttaggttc
    29641 atctaatgta aggctggcta ctttaaaaag aattcgcact ggtgatataa tcatagttca
    29701 gaaactttat aatttattat tgtgtaatca agttattatt ggggattata ttgtgaatga
    29761 taataatgag gcaaaaatta atctgtcaga aagtaatggt gagtcagaac acacagaagt
    29821 ttctttggca ttattcaatt atgatgatat caatgtaaaa gtggacttta ttcttttaga
    29881 aaaaaatatg acaatcaatg aactaaaaat gtatgtagaa aacgaattat tcaagtttcc
    29941 cgatgacata gttaaacatg taaatattaa agtaaatggt tctttggttg ggcatgggga
    30001 acttgtttct attgaggatg gttatggtat cgagattagt tcttggatgg taaaggagta
    30061 atctcatgct gagtgacatg tccctcatcg ctacactttc tttttttacg ctattaccat
    30121 ttttagttgc tgctggtacc tgttatataa agttttctat tgtttttgta atggtacgaa
    30181 atgcacttgg tttgcagcag gttccatcaa atatgactct caatggtatt gcattgatta
    30241 tggctttgtt cgtgatgaaa cccattattg aagctggtta tgaaaattat ctaaatggcc
    30301 ctcaaaaatt tgatacaata tctgatattg tccgtttttc tgattctggc ttgatggaat
    30361 ataagcaata cttgaaaaag catacagatc ttgagttagc acgttttttc caaaggtctg
    30421 aagaagaaaa tgctgattta aagagtgcag agaataatga ttattcattg ttttccctct
    30481 tgcctgcata tgcgctaagt gaaatcaaag atgcgttcaa aataggtttt tatttatatt
    30541 tgccatttgt tgttgttgat cttgttattt caagcatttt acttgcactt ggtatgatga
    30601 tgatgagtcc aatcacaata tcagtaccaa taaaacttgt attatttgtt gcgcttgatg
    30661 ggtggggtat cctgtctaag gccttgattg aacaatatat caatgttcct gcttagatga
    30721 tggaggtaat gtgtctgata tagtttatat gggtaataag gctctttatt taatccttat
    30781 cttttcctta tggccagtag gtatagctac ggttattgga ttatctattg gtttgttaca
    30841 gacagtgact caactccaag agcagacact tccttttggt ataaagctta taggtgtctc
    30901 aatatctttg ctacttcttt ctggatggta tggtgaggtt ttattgtctt tttgtcatga
    30961 aataatgttt ttaattaaga gtggggtttg atggacattt caagctggtt cgaaagtatt
    31021 catgtgtttt taatactcct gaacggcgtt ttttttagat tggctccatt atttttcttt
    31081 cttccatttt taaataacgg tataatttct ccatctatta gaatacctgt gatttttctt
    31141 gttgcatcag gattaattac ttctggtaag gtagacatag gttcttctgt ttttgaacat
    31201 gtttatttcc ttatgttcaa ggaaataatt gttggcctcc ttctctcttt ttgcttgtct
    31261 cttccctttt ggatatttca tgctgttggt agcattattg acaaccagcg tggggcaacg
    31321 cttagtagtt caattgatcc tgccaatggt gttgatacgt ctgagttggc aaaatttttc
    31381 aatctttttt ctgcagttgt atttctatac agtggtggta tggtctttat tttagaatcc
    31441 atacaattgt cttataatat atgcccgtta ttttctcaat gttctttccg tgtctcaaat
    31501 atcttaacat ttctgacttt attggcaagt caggctgtta ttttagccag tcctgttatg
    31561 atagtattgt tactatcaga agtattactt ggtgtattat cgagatttgc tccgcagatg
    31621 aatgcttttt ccgtatcatt aactattaaa agtttacttg caatatttat tattttcatc
    31681 tgttcttcta ctatttactt ttctaaagtt caatttttcc tcggtgaaca taaatttttc
    31741 acaaatctat ttgttagata aaatattatg gcaaataaaa cagaaaagcc gacacctaaa
    31801 aaactaaagg atgccgcaaa aaaaggacag tcatttaaat ttaaggattt aacgactgtt
    31861 gttattattc tggtagggac atttactata atatcattct tttccttaag tgatgtaatg
    31921 ctcttataca gatatgtaat aattaatgac ttcgaaatta atgagggtaa atactttttt
    31981 gcagtggtta ttgtcttttt taagataatt ggcttcccac tttttttctg tgttctttcg
    32041 gctgtgttgc caacattggt tcaaacaaag tttgttcttg cgactaaagc tatcaagatt
    32101 gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat aaagacaata
    32161 aaagaatttt tcaaaagcat tctgcttctt attattctag cattaacaac ctatttcttt
    32221 tggattaatg accgaaaaat aattttttct caggtgtttt ctagtgttga tggcttatat
    32281 cttatttggg gggggctgtt taaggatata atattatttt tcttggcatt ttctattttt
    32341 gttattattc ttgactttgt gattgagttc attttataca tgaaagatat gatgatggat
    32401 aaacaggaga taaaaagaga atatatagag caagagggac actttgagac aaagtcgaga
    32461 aggcgtgagt tgcatatcga gattctttca gagcagacta aatctgatat acgtaattca
    32521 aaattagtgg taatgaaccc gactcatatt gcaattggta tttattttaa tccagaaata
    32581 gcgcctgcac cttttatttc tctcattgaa actaaccagt gtgccttggc tgtcagaaaa
    32641 tatgcaaatg aagttggtat accaactgtg cgtgatgtga aattagctag aaaactatat
    32701 aaaacacata caaaatatag ttttgttgat tttgaacact tggatgaagt cctacgtctt
    32761 attgtttggc ttgagcaggt tgaaaacact cattaataag taaaggagat gtgttatatg
    32821 ggagttaatt tctgtaataa aataggtatt gatcagtctg aatttgaaat agaatcttcc
    32881 atcattaact ccattgctaa tgaggtattg aacccaatat ctttccttag caataaggat
    32941 ataataaatg ttttactcag aaagatttca tctgaatgtg acctggttag aaaagacatt
    33001 tatcgttgtg ctctggagtt ggtcgttgaa aaaacacctg atgatttata atcattcata
    33061 ataactcttt tgctgatata aatatttttg ttgaagatat ttatgtagtc acaggatcaa
    33121 ttgtagaaaa ttgttatgtt tttgttagga actgcactaa ataatatatg ataagacagc
    33181 aaaaacgact gaccataatc cttcttttat taggagttga taagagagac tattcatcct
    33241 gtaatgttaa aactctgtta tatagtattc gcgattatgc aaagtctgta aatgatcatg
    33301 agatactaac cgagagtaat cgtttattat ctcattgcat ttcagatagt aacggagctt
    33361 tctttaaaag tagtaaatac gttcccctta agtacttacg taaaaggaga atagcacgaa
    33421 agataccaaa tgattagcac tttgctagta agcttgctgg taaactatat tgaccgttgg
    33481 gtttgttttg agactgtaat ttaaattgtg taattgcctg tttttgatat gtttactcca
    33541 acaacggaga caggtaaatt atggacgaaa agaagcttaa agcccttgcg gctgaactgg
    33601 ctaaaggcct caaaactgag gccgacctca atcagttttc ccgtatgctg acgaaatatc
    33661 ccggctctta aaacgatagt ggctcaggct ttcatttttc agcgttcccc agaag
//
LOCUS       DAJTVJ010000037        32534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000037 DAJTVJ010000000
VERSION     DAJTVJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.037"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..95
                     /locus_tag="OO448_001681"
     CDS             <1..95
                     /locus_tag="OO448_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6336916.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(137..1213)
                     /gene="glpQ"
                     /locus_tag="OO448_001682"
     CDS             complement(137..1213)
                     /gene="glpQ"
                     /locus_tag="OO448_001682"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6336917.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1218..2576)
                     /gene="glpT"
                     /locus_tag="OO448_001683"
     CDS             complement(1218..2576)
                     /gene="glpT"
                     /locus_tag="OO448_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6336918.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2849..4477
                     /gene="glpA"
                     /locus_tag="OO448_001684"
     CDS             2849..4477
                     /gene="glpA"
                     /locus_tag="OO448_001684"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6336919.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4467..5726
                     /gene="glpB"
                     /locus_tag="OO448_001685"
     CDS             4467..5726
                     /gene="glpB"
                     /locus_tag="OO448_001685"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6336920.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5723..6913
                     /gene="glpC"
                     /locus_tag="OO448_001686"
     CDS             5723..6913
                     /gene="glpC"
                     /locus_tag="OO448_001686"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6336921.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7106..8008
                     /locus_tag="OO448_001687"
     CDS             7106..8008
                     /locus_tag="OO448_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6336922.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8049..8852)
                     /gene="yfaU"
                     /locus_tag="OO448_001688"
     CDS             complement(8049..8852)
                     /gene="yfaU"
                     /locus_tag="OO448_001688"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR6336923.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8870..10159)
                     /locus_tag="OO448_001689"
     CDS             complement(8870..10159)
                     /locus_tag="OO448_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6336924.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10216..11420)
                     /gene="rhmD"
                     /locus_tag="OO448_001690"
                     /pseudo
     CDS             complement(10216..11420)
                     /gene="rhmD"
                     /locus_tag="OO448_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11435..12217)
                     /locus_tag="OO448_001691"
     CDS             complement(11435..12217)
                     /locus_tag="OO448_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR6336925.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12437..13639)
                     /locus_tag="OO448_001692"
     CDS             complement(12437..13639)
                     /locus_tag="OO448_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6336926.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13739..14281)
                     /locus_tag="OO448_001693"
     CDS             complement(13739..14281)
                     /locus_tag="OO448_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336927.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14560..14985
                     /gene="nudI"
                     /locus_tag="OO448_001694"
     CDS             14560..14985
                     /gene="nudI"
                     /locus_tag="OO448_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6336928.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15024..15626)
                     /locus_tag="OO448_001695"
     CDS             complement(15024..15626)
                     /locus_tag="OO448_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6336929.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15934..17073
                     /gene="arnB"
                     /locus_tag="OO448_001696"
     CDS             15934..17073
                     /gene="arnB"
                     /locus_tag="OO448_001696"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6336930.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNRERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17077..18045
                     /gene="arnC"
                     /locus_tag="OO448_001697"
     CDS             17077..18045
                     /gene="arnC"
                     /locus_tag="OO448_001697"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6336931.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18045..20027
                     /gene="arnA"
                     /locus_tag="OO448_001698"
     CDS             18045..20027
                     /gene="arnA"
                     /locus_tag="OO448_001698"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6336932.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20024..20914
                     /locus_tag="OO448_001699"
     CDS             20024..20914
                     /locus_tag="OO448_001699"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6336933.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20914..22566
                     /gene="arnT"
                     /locus_tag="OO448_001700"
     CDS             20914..22566
                     /gene="arnT"
                     /locus_tag="OO448_001700"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6336934.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSYFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22563..22898
                     /gene="arnE"
                     /locus_tag="OO448_001701"
     CDS             22563..22898
                     /gene="arnE"
                     /locus_tag="OO448_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6336935.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22898..23284
                     /gene="arnF"
                     /locus_tag="OO448_001702"
     CDS             22898..23284
                     /gene="arnF"
                     /locus_tag="OO448_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6336936.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23278..23544)
                     /gene="pmrD"
                     /locus_tag="OO448_001703"
     CDS             complement(23278..23544)
                     /gene="pmrD"
                     /locus_tag="OO448_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6336937.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23588..25009)
                     /gene="menE"
                     /locus_tag="OO448_001704"
     CDS             complement(23588..25009)
                     /gene="menE"
                     /locus_tag="OO448_001704"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6336938.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25006..25968)
                     /gene="menC"
                     /locus_tag="OO448_001705"
     CDS             complement(25006..25968)
                     /gene="menC"
                     /locus_tag="OO448_001705"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6336939.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25968..26825)
                     /gene="menB"
                     /locus_tag="OO448_001706"
     CDS             complement(25968..26825)
                     /gene="menB"
                     /locus_tag="OO448_001706"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6336940.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26840..27598)
                     /gene="menH"
                     /locus_tag="OO448_001707"
     CDS             complement(26840..27598)
                     /gene="menH"
                     /locus_tag="OO448_001707"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6336941.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27595..29265)
                     /gene="menD"
                     /locus_tag="OO448_001708"
     CDS             complement(27595..29265)
                     /gene="menD"
                     /locus_tag="OO448_001708"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6336942.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29354..30649)
                     /gene="menF"
                     /locus_tag="OO448_001709"
     CDS             complement(29354..30649)
                     /gene="menF"
                     /locus_tag="OO448_001709"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6336943.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30728..31033)
                     /gene="elaB"
                     /locus_tag="OO448_001710"
     CDS             complement(30728..31033)
                     /gene="elaB"
                     /locus_tag="OO448_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6336944.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31088..31549)
                     /locus_tag="OO448_001711"
                     /pseudo
     CDS             complement(31088..31549)
                     /locus_tag="OO448_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31614..32531
                     /gene="rbn"
                     /locus_tag="OO448_001712"
     CDS             31614..32531
                     /gene="rbn"
                     /locus_tag="OO448_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6336945.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7898 a         8804 c         8138 g         7694 t
ORIGIN      
        1 tttcgttgtt ggctaaagaa aagccggtag aaggcagtcc actgttttta ctacaaatgt
       61 gcatggaaca attagtggcg attggaaaaa tttgatatga gacaggatgg cgcatgaacg
      121 ccatcctgca gtagcattac tctttattaa ggaattttac tgccttatca gggaaatcag
      181 taaacagccc atttacaccc gctttgttat acagagcatc atataactga ttcacatcag
      241 tagtgtattc aggcagttta tccgaccgca cggtataagg atgcactacc agtttattct
      301 gctgagcatc ttgcaccatg ccagtgagtt tgatattacc cggctgcgat gtttcctcaa
      361 tcaacatatg gtaatccggc ccgataccgt cggcatattc cgcgacttgc ttcatggcac
      421 ctggcttaaa catccagtca tagctgtaat taacccagct tccgtccggc tgtttctgct
      481 gcgtttcatt ccagtcggta taggcaatca gctgtaccag attgagatcc atgcccattt
      541 taggttccag ctcattctta atacgcttca gctcatcagc atcaaaacat tgcaaataaa
      601 ctttatcgtc cttaccggtg taaccatatt tcttcagcac ttccagcgtt tttgccgcaa
      661 tatccttccc ttcctgatga tggaaccacg gcgctttgat ttctggatag ataccgatat
      721 ttttcccggt agagtgattt aacccctgaa caaattcaat ctcttcttca aaggtgtgcc
      781 cccggaagtc ggacttaccc attgggaaac gccccggata agtctgtact tttttaccgt
      841 tttcaatatc gaaaccttcg gtaaatttca gcgacttaat ttcatccagc gtgaaatcta
      901 tcgcgtagta acgaccgtct ttgcgcgccc gatccgggaa acgatcggca acatcagtaa
      961 cacgatcgag ataatggtca tgcagaacaa ccagatggtc gtctttggtc atcaccaaat
     1021 cctgttccag ataatccgct ccctgcgcat acgccatcgc ttttgctggc agcgtatgct
     1081 ccggcaaata tccactggca ccgcgatggg cgatgactat tttttcgttg ctgtccgccg
     1141 ccattgcact gcttcccatg actatagtgc tcatcatgat cgccatgcta aggtttttca
     1201 gcttcaattt catgccatta gcctccgttg cgtttttgca gtaattgttc atggcgacgt
     1261 ttttcgccaa tcatcacaac aatcaacaag ataaccgcca gaatgctgcc gccaatcatt
     1321 accataaagc cgccatccca gccgaagaag tccacggtgt agccaacaat cgcgctcgcc
     1381 gccaccgaac cgcccaggta accaaacagc ccggtaaagc ccgctgccgt acctgccgct
     1441 tttttcggtg ccagttccag cgcatgcaga ccgatcagca tcacaggacc gtagatcagg
     1501 aagccgataa caatcataca aatcatatcg acggttgggt tacctgccgg gttcatccag
     1561 taaacgatag tcgcgatggt caccagtgtc ataaagaaaa cgccggttgc cccacggttg
     1621 ccacggaaga ctttatccga catccagccg cacagcagag tgcccggaat acctgcatat
     1681 tcataaagga agtaggccca ggaggattta tccagcgcga aatgcttaac ctctttcaga
     1741 taagtcggtg accagtcgag gatgccgtaa cgcagcagat aaacgaacac gttggcgatg
     1801 gcgatatacc acagcagttt gttcggcagt acgtactgca tgaagatttg cttcgccgtc
     1861 agctcctgtt ccgctttttc gttatagtcg tccggataat catttttgta ctcttcgatc
     1921 ggcggcaagc cacaggattg cggggtatcg cgcatcatcg caaaggcgaa taatgccacc
     1981 agaatggcgc agaaagcagg catatagagc gccgcatgcc agtcattgaa ccaggccatc
     2041 cccagcagga acagcagcgg cggaatacca ccaccgacgt tgtgcgcaca gttccacact
     2101 gacacaatgc cgccacgttc tttctgcgac caccagtgca ccatagtacg accacacggc
     2161 ggccacccca tcccctggaa ccaaccgcag aggaacaaca gtacaaacat caccgcaatg
     2221 ctcgacgtcg cccatggcac aaagcccata aacaacatca ctgccgccgc cagaatcaaa
     2281 cctgcgggca ggaaaacgcg cggattcgag cgatccgata ccgaacccat gatgaatttc
     2341 gaaaatccat aagcaatcga gatccccgaa agggcaaaac ctaaatcacc gcgtgagaat
     2401 ccctgctcaa ccagataagg catagcaagc gcaaagttct tacgaaccaa atagtaagcc
     2461 gcatagccaa agaatatccc caggaaaatt tgccagcgca atcgacgata agtcggatcg
     2521 atctccgcgg caggtaagcg cgctttgtgt ggcgctggtt taaaaatact caacattgat
     2581 agcctccgtg gcccgtggtc ttatttatga ttaacagcct gattcagtga gagaacctgc
     2641 cgtttcttga gttgccgcga tgttaagaaa acattcataa attaaatgtg aattgccgca
     2701 cacattatta aataagattt acaaaatgtt caaaatgacg catgaaatca cgcttcactt
     2761 tcgaatcatg agcgaatatg cgcgaaatca aacaattcat gtttttacta tggctaaatg
     2821 gtaaaaaacg aacttcagag ggataacaat gaaaactcgc gactcgcaat caagtgacgt
     2881 gattatcatt ggcggcggcg caacgggagc cgggattgcc cgcgactgtg ccctgcgcgg
     2941 gctgcgcgtg attttggttg agcgccacga catcgcaacc ggtgccaccg ggcgtaacca
     3001 cggcctgcta cacagcggtg cgcgctatgc ggtaaccgat gcggaatcgg cccgcgaatg
     3061 cattagtgaa aaccagatcc tgaaacgcat tgcacgtcac tgcgttgaac caaccaacgg
     3121 cctgtttatt accctgccgg aagatgacct ctccttccag gccactttta ttcgcgcctg
     3181 cgaagaagca gggatcagcg cagaagctat agacccgcag caagcgcgca ttatcgaacc
     3241 tgccgttaac ccggcattga ttggcgcggt gaaagttccg gatggcaccg ttgatccatt
     3301 tcgtctgacc gcagcaaaca tgctggatgc caaagaacac ggtgccgtta tccttaccgc
     3361 tcatgaagtc acgggactga ttcgtgaagg cgcgacggtg tgcggtgttc gtgtacgtaa
     3421 ccatctcacc ggcgaaactc aggcccttca tgcgcctgtc gtggttaatg ccgccgggat
     3481 ctgggggcaa cacattgccg aatatgccga tctgcgcatt cgcatgttcc cggcgaaagg
     3541 atcgctgctg atcatggatc accgcattaa ccagcatgtg atcaaccgct gccgtaaacc
     3601 ttccgacgcc gatattctgg tgcctggcga taccatttcg ctgattggca ccacctcttt
     3661 acgtattgat tacaacgaga ttgacgataa tcgagtgacg gcagaagagg ttgatattct
     3721 gctgcgtgaa ggggaaaaac tggcccccgt gatggcgaaa acgcgcattt tgcgggccta
     3781 ttctggcgtg cgcccgctgg ttgccagcga tgacgacccg agcggacgta acgtcagccg
     3841 tggcatcgtg ctgctcgacc atgctgaacg cgatggtctg gacggattta tcaccatcac
     3901 cggtggcaaa ctgatgacct atcggctgat ggctgaatgg gctaccgacg ccgtgtgccg
     3961 caaactgggc aacacgcgcc cctgtacgac cgccgatctg gcacttcctg gttcacagga
     4021 acccgctgaa gttaccttgc gtaaagtcat ctccctgcct gccccgctgc gcggttctgc
     4081 ggtttatcgt catggcgatc gcacgcctgc ctggctgagc gaaggtcgtc tgcaccgtag
     4141 cctggtatgt gagtgcgaag cggtaacagc gggtgaagtg cagtacgcgg tagaaaattt
     4201 aaacgttaat agcctgctgg atttacgccg tcgtacccgt gtggggatgg gtacctgcca
     4261 gggcgaactc tgcgcctgcc gcgctgccgg actgctgcaa cgttttaacg tcacgacgtc
     4321 cgcgcaatct atcgagcaac tttccacctt ccttaacgaa cgctggaaag gcgtgcaacc
     4381 catcgcctgg ggagatgcac tgcgcgaaag cgaatttacc cgctgggttt atcagggact
     4441 gtgtggtctg gagaaggagc agaaagatgc gctttgatac tgtcattatg ggcggcggcc
     4501 tcgccggatt actctgtggc ctgcaactgc aaaaacacgg cctgcgctgt gccattgtca
     4561 cgcgtggtca aagcgcgctg catttctcat ccggttcgct ggatttgctg agccatctgc
     4621 ctgatggtca accggtgaca gacattcaca gtggactgga atctttgcgt cagcaggcac
     4681 cagcccatcc ttacagtctt ctcgaaccac aacgcgtgct cgatctcgct tgccaggcgc
     4741 aggcattgat cgctgaaagc ggtgcgcaat tgcagggcag cgtggaactg gcccatcagc
     4801 gcgttacccc gctcggcact ttgcgtgcaa cctggttaag ttcgccagaa gttcccgtct
     4861 ggccgctacc cgcgaagaag atatgtgtag tggggataag cgggctgatg gatttccagg
     4921 cgcatcttgc ggcggcttcg ttgcgcgaac tggatctttc ggttgaaacc gcagaaatag
     4981 agctgccgga actggatgtc ctgcgcaata acgccaccga atttcgcgcg gtgaatatcg
     5041 cccgtttcct tgataatgaa gaaaactggc cgctgttact tgatgcgctt attcctgtcg
     5101 ccaatacctg cgaaatgatc ctgatgcccg cctgcttcgg cctggccgat gacaaactgt
     5161 ggcgttggtt gaatgaaaaa ctaccttgtt cactgatgct tttgccaacg ctgccgcctt
     5221 ccgtgctggg cattcgtctg caaaaccagt tacagcgcca gtttgtgcgc cagggtggcg
     5281 tgtggatgcc gggcgatgaa gtgaaaaaag tgacctgtaa aaatggcgta gtgaatgaaa
     5341 tctggacccg caatcacgcc gatattccgc tacgtccacg tttcgcggtt ctcgccagcg
     5401 gcagtttctt tagtggcgga ctggtagcgg aacgtaacgg cattcgcgag ccgattctcg
     5461 gccttgatgt gctacaaacc gccacgcggg gtgaatggta taagggcgat ttttttgcgc
     5521 cgcaaccgtg gcagcagttc ggtgtaacca ctgatgagac gctacgcccg tcgcaggcag
     5581 ggcaaaccat tgagaacctg tttgccatcg gttcggtgct gggcggattt gatcccatcg
     5641 cccaaggatg cggcggcggt gtttgtgccg tcagtgcttt acatgccgct caacagattg
     5701 cccaacgcgc aggaggccaa caatgaacga caccagcttc gaaaactgca ttaagtgcac
     5761 cgtctgcacc accgcctgcc cggtgagccg ggtgaatccc ggttatccag ggccaaaaca
     5821 agccgggccg gatggcgagc gtctgcgttt gaaagatggc gcactgtatg acgaggcgct
     5881 gaaatattgc atcaactgca aacgttgtga agtcgcctgc ccgtccgatg tgaagattgg
     5941 cgatattatc cagcgcgcgc gggcaaaata tgacaccacg cgcccgtcgc tgcgtaattt
     6001 tgtgttgagt cataccgacc tgatgggtag cgtttccacg ccgttcgcac caatcgtcaa
     6061 caccgctacc tcgctgaaac cggtgcggca gctgcttgat gcggcgttaa aaatcgatca
     6121 tcgtcgcacg ctaccgaaat actccttcgg cacgttccgt cgctggtatc gcagcatagc
     6181 ggctcagcaa gcacaatata aagaccaggt cgctttcttt cacggctgct tcgttaacta
     6241 caaccatccg cagttaggta aagatttaat taaagtgctc aacgcaatgg gtatcggtgt
     6301 acaactgctc agcaaagaaa aatgctgcgg cgtaccgcta atcgccaacg gctttaccga
     6361 taaagcacgc aaacaggcaa ttacgaatgt agagtcgatc cgcgaagctg tgggagtaaa
     6421 aggcattccg gtgattgcca cctcctcaac ctgtacattt gccctgcgcg acgaataccc
     6481 ggaagtgctg aatgtcgaca acaaaggctt gcgcgatcat atcgaactgg caacccgctg
     6541 gctgtggcgc aagctggacg aaggcaaaac gttaccgctg aaaccgctgc cgctgaaagt
     6601 ggtttatcac actccgtgcc atatggaaaa aatgggctgg acgctctaca ccctggagct
     6661 gttgcgtaaa atcccggggc ttgagttaac ggtgctggat tcccagtgct gcggtattgc
     6721 gggtacttac ggtttcaaaa aagagaacta ccccacctca caagccatcg gcgcaccact
     6781 gttccgccag atagaagaaa gcggcgcaga tctggtggtc accgactgcg aaacctgtaa
     6841 atggcagatt gagatgtcca caagtcttcg ctgcgaacat ccgattacgc tactggccca
     6901 ggcgctggct taaactcctt tctgatgccc ggtaagcatg tggttaccgg gcatttttgc
     6961 gtacacgatt ccgtgcccaa tgtatgcgtt gcaacgcagt gaaaattcct ctgaaaacgt
     7021 ctcgcaaagg ctgaaactgg cagatgtcaa aggcctggga taaccgtaat gtcgcgtcat
     7081 cataaatatc aggtgacgga caaccatgac cgaatcaaca acctcctccc cgcatgatgc
     7141 ggtatttaaa acctttatgt tcacacccga aaccgcacgg gattttctcg aaatacattt
     7201 gccagaacca ctgcgcaagc tttgcaacct gcaaacctta cgcctggaac ccactagttt
     7261 tattgaaaaa agtttacgcg cttactactc ggatgttttg tggtccgtgg aaaccagcga
     7321 cggtgacggc tatatctact gcgtgattga acatcaaagc tctgcagaaa agaatatggc
     7381 ttttcggcta atgcgctatg ccactgccgc catgcagcgt cacctggata aaggctatga
     7441 cagagttccg ctggtggtgc cattgctgtt ttatcatggc gaaacatcgc cctacccgta
     7501 ctcacttaac tggctggatg agtttgacga tccgcaactt gcccggcagt tgtacaccga
     7561 agcttttccg ttggtggata tcaccatcgt acctgacgat gagatcatgc aacatcggcg
     7621 tatagctctg cttgaactga ttcaaaagca tattcgcgac cgcgatttaa tcggtatggt
     7681 cgacaggatc accacgcttt tggttaaagg cttcactaat gacagccagc tacaaacact
     7741 gtttaattat ctgctgcaat gcggcgatac ctcccgtttc acccgtttta ttgaggagat
     7801 ttccgaacgt tcaccactac aaaaggagag attgatgact attgctgagc ggctacggca
     7861 ggaaggacac caaattggct ggcagaaagg tatgcatgaa caagccatta aaattgcttt
     7921 gcgcatgctg gaacagggca ttgatcgtga ccaggtgctc gcggccaccc agctaagcga
     7981 agccgatctg gcagcgaata accactaatt aacacaggcc cacagccgat ccccatgggc
     8041 ctttgatatc aataactacc ttttatgcgt ggcccatttt tgcctgattt aaacatcgcc
     8101 agtcgttgat ccagggcatc gctgtagagc atcgtgtcaa cgccaacagc gacaaagttc
     8161 gctccccacg ccaggcattg ctgcgccata tcaggagcca cagccagaaa accagccgct
     8221 ttacccgcag cacggatccg ccgaatgctg gtttcaataa ttcgctgcac ttccgggtgc
     8281 ccggcgttat ccgggtagcc caacgacgca gaaagatccg ctggtccaat aaacacgcca
     8341 tcaatccctt cgacgtcgag gatctcgtcc aggttatcca gtgccgtttt actttccacc
     8401 tgcactaaca gacaaagcga atcgttgact tgcgccatgt aattctcaat gcgtccccag
     8461 cgcgcagccc gtgccacact ggccccaaca ccacgctcac cgtagggagg atagcgcgtg
     8521 gcagacgcca cctgacgagc ctgatcggca gtatcgacca tcgggatcaa tagagtttgc
     8581 gcgccaatat ccagaacttg tttaatcagc gatttactgc cttccaccgg acggatcacg
     8641 ggttggctgg catagggcgc taccgcctgt agctggtgat aaagatcctg aatggtgttt
     8701 ggcgcgtgct ccccgtcaat cagcaaccag tcataaccgg aagtagcggc aatttctgcc
     8761 atataagaag tagtcgagct taaccacaga ccaatttgca cttcgccctt gcgtaaacgt
     8821 tctttaaagg gattggttaa taatgcgttc atcgtgttcc ttaacctatt taatgatgtg
     8881 ccacgtcggt ctgcgcaaca gtacgattta cacgcagagt gaaaataatc agcgaaccga
     8941 tgaccgccac cgctgccagt gtcagtaatc ctgccgcatc gctggcaaat aacgtttctg
     9001 ctttcacgcg caggatcggg gcaataaacc cgcccactgc accaaacagg tttacaaaac
     9061 caatccccgc agccagcgcc gtaccggaaa gcagttgtgt cggcatcgtc cagaacaccg
     9121 gctgcacggc aataaacccg atagctgcaa cacacagcgc tacgatcgcc attactggag
     9181 aaagcagccc ggacagacca ataccaatgc ccgccgccag taatgtcagc gctgcgacat
     9241 tacgccgttc gccggtttta tcggaatagc gcggaataag ccaggtccca aacaaggccg
     9301 caacccacgg aatggcggtg accaccgacg ctgtaaagcc cacttttgtt cccagcaatg
     9361 ccgcaacctg ggtcggcagg aagaaaatta atccgtaaac cgccacctga atggtcaggt
     9421 agataatcgc tagttgccag actcggccat tacgcagcgc atcgctcagc cgagaagtca
     9481 ctttctgttg ttcttcactt gccagttgat tgataagcaa cgttttttct tgtttactca
     9541 gaaaacgtgc ctgctccggt gtgtcatcaa gccaaaagaa tgtgaatacc ccagcaccga
     9601 ctgccaacaa tccttcaatc acaaacatcc agaaccagcc gggatgcccc ataaatccat
     9661 gcatctccaa cagcgcgccg gaaagcggtg atcccagtgt taacgccagc ggtgcgccca
     9721 tatagaacag ccccataatg ctggcgcgat tacgctgcgg aaaccattgc gaggtgagat
     9781 aaatcatacc agggaaaaat ccggcctccg cagcaccaag cagagtgcga actatcagaa
     9841 atttcgcttc agtatcggcc catgccatgg ctgccgaaag aaatccccac agcagtgttg
     9901 tcgtaccaat ccaggttctg gcccccagtt tgcgcatcaa aagattcgcc ggaacaccca
     9961 gaaacgcata taccacaaag aaaatgcctg ctcccagcgc ataagcttca ttactcaacc
    10021 cggtatcaat ctggtaggtc tgtttggcaa aaccgatatt cgaacggtcg agaaacgcca
    10081 gcacatacag cgccaacata aacggaatta aacgcacacg gtttttcttc accacggcgt
    10141 caagcaaagc ggtgctcata aaaagctcct tagaatatgt gggcgttacc gttgtcacag
    10201 caacgcccgg agtgattagt ggctgtaggg gcgtttcaaa ttgcagtcac gattgagttc
    10261 gacgccaaaa ccgggtttat caagcactga tttatgaata cgaccattca ccggaaccgg
    10321 ctcattgagc agaatcgggt caaactgcgg acgcatcgtt gaacaatccg ggctggtcat
    10381 caggaattcg ctgaatggcg tattggtgaa ggtgatcacc gcatggtgag agtaaacaga
    10441 cgaaccgtgc ggcaccacca gttgccccct ggatttggcg attgcggcaa tttccaccag
    10501 cgtggttaaa ccaccgcacc agccaacatc cggctgcata atgtcgatac cggtttctga
    10561 aagcgtacga aaagattgca gtgtgccgtg gtgctcaccg ctggtgacca tcattccgac
    10621 tggcgcgttg cgtttcagtt cgcgataacc ttcatactgc tgtggcggca ggcactcttc
    10681 gatccatttc aggttatagg gcgcgcaagc gtgggccagt ttggtggcat agttcacgtc
    10741 ctgactcatc cagcagtcga gcattaacca gaaatcctca ccgcattttt cacgcatatc
    10801 cgcgaccata acggcatctt tgcggatccc cgcatcgcca tcatgtggcc cccagtgcgt
    10861 cggcattttg ccaccgataa agcccatctc ttttgccaga tccggacgcg cgcctgtggc
    10921 gtagaactga atctcatcac gaactgcgcc gcctaaaagt ttataaaccg gaagcccgac
    10981 cactttgccg aacagatccc acagagccag atcgacacag gaaatcgtgt tcatcaccag
    11041 gccaccagag ccggagtaat acagggtggc attgagcatt tgatcatgga tcagtttgat
    11101 atcactgaca catttaccct caatgaaacg gttaagatgt ttttcgacaa taaaacagcc
    11161 catttcaccg gctgtcgaaa cagcgaatcc ggtctggccg ttttctgctt cgacttcaac
    11221 aatcaacgtg ccaagaacgt taatgccaaa tgactggcgt gactgctcgt aatcgcggta
    11281 tttactcatc ggggtggcaa tatgatcgtc aatccagtga ttcgccccct ggtcgtgata
    11341 atcaccgcgc cagcaccttt ttctgctgtc gcaccgccag taaaccaggc gcgaacctgt
    11401 ttgatttttg gtagggtcat gatgttctcc attgttatga ggcctgtaag tcaaagggac
    11461 ttttccatcc caacagacgt gaaatatccc tggcgcaggc aatggccttg cccgccagat
    11521 aatcacggta ttcttcattg atttgtaagc gggtaccgac caccgagatc gcagcggtaa
    11581 gctcgttatt ggcgttaaac accggcgcag cgacacaacg gacatcgggg taatcttcgc
    11641 cgttgtcata gctccagccc tgacggcgaa tacgcgccag ttcttcgtga agttgctgtg
    11701 gatgagtaat cgttgtgggt gtcgcctgct cccagaccag cccttcgata atactttgct
    11761 gtaccgctgc aggttgccag gcaagcaggc atttacctat tccggagcga taaagcgaaa
    11821 ggctttttcc ttcatgggaa cgcacgctga tagtggctga tgactccact tttaaaatgt
    11881 aataagcgct gccgttatca ataatcccca ggtgacataa cagcccggtg gtatccatca
    11941 gttgcgtcaa acgcggtcga gccagttccc ggagatccat cttgcttaac gcatgaccag
    12001 aaagctccac cagcctggtc cacaaacaaa aattttcctg gtgatcaagg ctaagaaaac
    12061 gctgacgcct gagttcatta agcagcaaat aggccgtgct tttagggatt cccaatgtgt
    12121 caatgatcgt cgcagcacta caggggccaa tgcgggcaat caaattgaga atatcaatag
    12181 cccgtgtcag ggcgggaact ttgcttgatt ccaacatact ggactccagt cttagatact
    12241 ggaatcagtc tcgttgctca gggggagtaa aattgtgaag cgcatcaaat cccgctcctc
    12301 gtccagcgga ctggagaaat tacgcacaaa tttgtgaatc agtgcacgga ttttttctta
    12361 ttgagtacaa caggttggac aagttttgcc ggagggggaa tgtgccggtg cagtaaaccg
    12421 gcacagagtg gcggaatcag acagataagg tcatggactc aacaacctca atccagccat
    12481 gctcactggc gatatcctgg ccgtttaacc aacggcgcag catattcagt gccatcattg
    12541 cgcacacttc ctgacggata gccaggctgt agcgtgtagt gctgaaacgc acacgcagag
    12601 cgaaagtgcc gtctggcgtg gctagcgcaa agttgagatg ctcgttctcg aaacccgaaa
    12661 cagccagtgc cagcccggca aaatggttgg cccgccgttc tgtaatccag tgcgcagttt
    12721 gcgccagggt ttcttcctgt gaaggaacca cttcacacgc cagcaatgga gcacctgcgc
    12781 gagaaagttg caaagccaat aaaccaccgg tgaattgctc gctcaacgtc aggctgaact
    12841 ggcggttttg caattcgcga ctgatctgcg cgggcagtcc ttcggtgcct tcgaaaatca
    12901 cgctctgtcc ggcaacacgt ttaacgtcca gccacagttt ttccatcgcc tgttgctcgc
    12961 ttgccggtcc ggtgagtttc agttcgatga tcggcattga ggagcgatag cccattgtta
    13021 cgcccggcgg cagttgtaga gtgtccaggc tttgtgccag atcgctttcc gaacgaccaa
    13081 aggtagttaa acgcagacaa accggcgact gtggtaaaga aaaacgctcg cgcaggcgcg
    13141 gcaggatttc gtgctcgacc atcaccttaa attctgacgg tacgccggga gtaaagaaca
    13201 tcaggcaacg attaagccgc acggcaaaac cacaggcggt gcctaccggg ttattgataa
    13261 attcagcact ggcaggcagc tccgcttgtt tacggttgct cggtgccatt actcgtccac
    13321 gttcgtggaa atagcgttcc atctctttga gccaggcttc atgcagcacc aggccttcac
    13381 cttttgctgt cgcagcggcg agtgcactta aatcatcact ggtcggtccc agcccgccgt
    13441 taacgatcag cacatcggcg tgctgactac gttcgcgaag aatggtgact aagtcatcaa
    13501 ggttatcccc caccgtattg cggcgagata atggcaaccc ctgatgaaag aaaaaatcgg
    13561 ccagccaggc agcgttagtg tcaacgattt gcccgtgtaa cacttcatcc ccggtggata
    13621 acatttccac ttttaacatg gttatctcct gcttttgtta tcgaatcact ataacgcaaa
    13681 gggagataag gcgtagtaag aaactgaatg gcgcgacaga atgccgcgcc ggaagggatc
    13741 agaaactggc gttaacccca acatacgggc cgtcagccac ggcgttgtcg cggttaccgt
    13801 ctttacccga cagattcagg tagcgataac ccgcctcaat actgactgga tgcataatgg
    13861 tgtaacgcgc gccagcattc gcttcctcat aacttttaat accgctggag agtgaatccg
    13921 gagagtagta atactcgcca aacaaacgga agctgttgcc aattttccac tgcaaaccac
    13981 ctcctaccgc tgcggcataa ccttcatcgc cgtaattcgg gttggtgtaa acgccttttc
    14041 cgccaacggt cgccattaac ggcccgagag gcagattcaa ccccagcccc acgccagcga
    14101 cgtcaccgtc gtcgtcgtta tgtgtccagt taccgcttaa cgccaggccc gtcgattcag
    14161 tcccaaaacc gacaccaata ttggtgtatt ctttacccgc ctgaccgctg atgctcattg
    14221 cattgaccga tgcagaaacc aacagcattc cggccagacc tgctagcgca atttttttca
    14281 ttatcccctt tcccacaagc aattaatcaa aattcccaaa gctgcgggat tgtactgcct
    14341 ggcacagagg attcaatgag cgaaaaaagg gatctagtca cttattgtaa ctaaatactt
    14401 cttaaataat taaacgccga aaaaataaac acactttacc gatacacaca aaaataataa
    14461 taagtgcagt tatcatcgca aaagagatta ctgtcacttt cacatatttc tgagttaccg
    14521 tattctcatg gtttcttttt atttataagg aatacccctg tgcgacaacg gactattgta
    14581 tgccctttga ttcaaaatga tggtgcttat ttgctgtgca aaatggccga cgatcgcggc
    14641 gttttccccg gtcaatgggc gctttcgggt ggcggcgtgg agtctggcga acgaattgaa
    14701 gaggcactac gccgcgaaat tcgcgaagaa ctgggagaac agctgctttt gacagaaatc
    14761 acgccgtgga ccttcagcga tgatattcgc accaagacgt atgcagatgg tcgcaaggaa
    14821 gagatttata tgatttacct gatttttgac tgcgtttctg ccaaccgaga agtgaaaata
    14881 aacgaagagt ttcaggacta cgcgtgggta aaacctgaag atctggtgca ttatgattta
    14941 aatgttgcca cccgaaaaac gttacgtttg aaaggtcttc tttaacaatg atgacagcca
    15001 tcactggctg tcagattgaa aatttagtgg ttaacgaatt ccccatccag ataaactttg
    15061 cctttttcca catgcatgac taaaccatcc agataatcag gtttaaatgt cgcatcacgc
    15121 ttatttttag caatatatgt caggcaatga ttatgggtga aaataacgat atttttatca
    15181 ggcgctttgc tttgtaagtc cttaattgca ctataaatct cattaccgca ctgaagaagt
    15241 cgtttatcta ccgtcagttt tttacccgcg gaaaaccagg tcgccgactg aatggtccgg
    15301 acggtattac tggaataaag atcgaaatca gggatgtcag cgctaaaagc gttgcccagt
    15361 tcacgggcat cctgggtacc tttaaccgta atacctgttt tatctgacaa gcattgattg
    15421 gttgaacgat cgcaacgttc agcatggcga aacaaaacga caaccgggtg ctgctgtgcc
    15481 agtctggcca atgttttatt gtcgatacgt ggcaaaccat tgctactcca ggcggcatga
    15541 gtacccagtc cggcaattgc agcgagcgcc agtaaaatga tgaaatattt ttttgacttc
    15601 aatgatgagc ggcaaaaagc taacacgtaa actccaccta tagacaagcg caaccagaca
    15661 attaccgtga aattgagcga catttctggc gataattagc agttggtgta atattaaaaa
    15721 tcctacgatg tcggcaaaat gcctcaaaat tttgccaaat gcaaagccta aataagaaaa
    15781 aatataaaaa tttcaatatt tacgtctaat attagtttct taaggttaag ttaatattct
    15841 atccttaaaa tttcgctcca aatggcaaaa tatacacaac actctttata gcaaatataa
    15901 gtggacaggt attcaatggc ggaaggaaaa gcaatgtcag aatttttgcc tttttcgcga
    15961 ccagcaatgg gcgtggagga actcgctgca gttaaagagg ttctcgaatc cggttggatc
    16021 acaaccggtc cgaagaatca ggcgcttgag caagcctttt gccagttgac gggaaatcag
    16081 catgccatcg cggtcagttc agccaccgcc ggaatgcata tcacgctaat ggcgttggaa
    16141 attggcaagg gcgatgaagt gattacgcct tccctgacct gggtttcaac cctcaatatg
    16201 atttccttgt tgggtgcaac gccggtaatg gtggatgtcg accgcgatac gctgatggtc
    16261 acgcctgaag cgatcgagtc agccattacg ccacgcacta aagccatcat tccggtgcat
    16321 tatgccggtg cgccagcaga tattgacgcc attcgcgcca ttggcgaacg ttacggcatc
    16381 gcagttatcg aagatgctgc ccatgccgtc ggtacgtatt acaaagggcg acatattggc
    16441 gcaaaaggta ccgctatttt ttcatttcat gccattaaaa atattacctg tgcggaaggt
    16501 ggcctgattg taactgataa tgaaaacctt gcccgccagc tacggatgct gaaatttcac
    16561 ggtctgggtg tcgatgccta tgacagacaa acctggggcc gtgcaccgca ggctgaagtc
    16621 ttaacaccgg gctataagta caatctgacc gatattaacg ccgcgattgc cctgacacag
    16681 ttagcaaaat tagagcacct caacacccat cggcgcgaaa ttgcccagca atatcagcaa
    16741 gcactggcag ctctcccctt tcagccatta agccttcccg cctggccgca cgttcacgcc
    16801 tggcatctgt ttattattcg tgtcgatgaa caacgttgtg gtatcagtcg cgatgcgttg
    16861 atggaagcgt taaaagaaag aggcattggt accgggttac atttccgcgc cgctcacaca
    16921 caaaaatatt atcgcgagcg ttttcccacg ttgtcgttac cgaataccga atggaataga
    16981 gaacgcatct gttctttgcc gctgttcccg gatatgacta ccgccgatgc cgaccgtgtc
    17041 atcacagccc ttcagcaact cgcaggacaa taagccatgt ttgaaatcca ccctgttaag
    17101 aaagtctcgg tggttattcc cgtttataac gagcaggaaa gcttaccgga attaatcagg
    17161 cgcaccacca cagcctgtga atcgttgggg aaagagtatg agatcctgct gattgatgac
    17221 ggcagtagcg ataattccgc gcatatgctg gtcgaagcct cacaagcgga gaacagccat
    17281 attgtgtcta ttttgcttaa ccgcaattac gggcaacatt cagcgattat ggcgggattc
    17341 agtcacgtta ctggcgactt aattattacc cttgatgccg atctccagaa tccgccagaa
    17401 gaaatccccc gcctggtggc aaaagccgat gaaggttacg acgtggtagg gactgtacgc
    17461 cagaaccgcc aggacagctg gtttcgtaaa acagcttcga agatgattaa caggcttatt
    17521 cagcgcacca ccggcaaagc gatgggtgac tacggttgta tgctgcgcgc ctatcgccgt
    17581 catattgtcg atgcgatgtt gcactgccat gaacgtagca cctttatccc gattctggcg
    17641 aatatcttcg cccgccgtgc cattgaaatt ccagtacatc atgccgagcg tgagtttggt
    17701 gaatccaaat acagttttat gcgcctgatt aatttgatgt acgacctggt gacctgcctt
    17761 accacaacgc cgctacgtat gctgagtctg ctcggcagca ttattgcgat tggaggtttt
    17821 agcattgcgg tgctgctggt gattttacgc ctgaccttcg gaccacaatg ggcggcagaa
    17881 ggcgtcttta tgctatttgc cgtgctgttt acttttattg gcgctcagtt tatcggcatg
    17941 ggattgctcg gtgaatatat cggcaggatc tacaccgatg tccgcgcccg cccccgctat
    18001 tttgttcagc aagttatccg tccatccagc aaggaaaatg aataatgaaa accgtcgttt
    18061 ttgcctacca cgatatggga tgcctcggta ttgaagctct gctggctgcc ggttacgaaa
    18121 ttagcgccat ttttacccat accgataatc ccgctgaaaa agccttttat ggttcggtgg
    18181 ctcgtctggc ggcggaaaga ggcattccag tttatgcgcc ggataacgtt aatcatccgc
    18241 tgtgggtgga acgcattgcc caactctcac cagaggtgat tttctctttt tattatcgcc
    18301 atcttatttg cgacgagatt ttgcagctcg ctcccgcagg tgcatttaat ctgcacggtt
    18361 cgctgttacc aaaatatcgt ggtcgcgcgc cgctgaactg ggtgctggtg aacggtgaaa
    18421 cggaaacggg cgttacattg caccgaatgg taaaacgtgc cgatgctggg gccattgtgg
    18481 cgcaactgcg cgttgccatt gcgccagacg atatcgctat tacgctgcat cataaattgt
    18541 gccatgccgc gcgccagcta ctggaacaga cattacccgc cattaaacac ggtaatattc
    18601 tggaaattgc ccagcgcgaa aacgaagcca cctgttttgg tcacagaacg ccagatgaca
    18661 gtttccttga atggcataaa ccggcatccg tactgcacaa catggtacgt gccgttgccg
    18721 atccgtggcc gggtgccttc agctatgttg gcaatcagaa attcaccgtc tggtcgtcgc
    18781 gtgttcatcc tcatgccagc aaagcacagc cggggagcgt gatttctgtt gcgccactgc
    18841 tgattgcctg tggcgatggc gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta
    18901 ctatgcaggg ctcgcaatta gcgcagatgc tgggcctggt gcaaggttca cgcttgaata
    18961 gccagcctgc ctgcaccgcc cgacgccgta cccgggtact catcctcggg gtgaatggct
    19021 ttattggcaa ccatctgaca gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc
    19081 tggatattgg cagcgatgcg ataagccgtt ttctgaatca tccgcatttt cactttgtcg
    19141 aaggcgatat cagtattcat tccgaatgga ttgagtatca cgtcaaaaaa tgtgatgtcg
    19201 tcttgccgct ggtggcgata gccacgccga ttgaatatac ccgcaacccg ctgcgcgtat
    19261 ttgaactcga ttttgaagag aatctgcgca ttatccgcta ctgcgttaag taccgtaagc
    19321 gaatcatctt cccgtcaact tcagaagttt atgggatgtg tagcgataaa tacttcgatg
    19381 aggaccattc taatttaatc gtcggcccgg tgaataaacc acgctggatt tattcggtgt
    19441 caaaacaatt acttgatcgg gtgatctggg cctatggcga aaaagagggt ttacagttca
    19501 ccctcttccg cccgtttaac tggatgggac cacgactgga taaccttaat gcagcgcgaa
    19561 ttggcagctc ccgcgctatt acgcaactca ttctcaatct ggtagaaggt tcaccaatta
    19621 agctaattga tggcggaaaa caaaaacgct gctttactga tatccgggac ggtatcgagg
    19681 cgttgtaccg cattatcgaa aatgcgggaa atcgctgcga cggcgaaatt atcaacattg
    19741 gcaatcctga gaacgaagcg agcattgagg aactgggcga gatgctgctg gcgagcttcg
    19801 aaaaacatcc gctgcgccat cacttcccac cgtttgcggg ctttcgtgtt gtcgaaagta
    19861 gcagctacta cggcaaagga tatcaggacg tagagcatcg taaaccgagc atccgcaatg
    19921 cccgccgctg cctggactgg gaaccgaaaa ttgatatgca ggaaaccatc gacgaaacgc
    19981 tggatttctt cctgcgcacc gttgatctta cggataaacc atcatgacca aagtcggctt
    20041 acgcattgat gtcgatacct ttcgtggcac ccgtgaaggc gtgccacgtc tgctggaaat
    20101 cttgagtaag cataatattc aggccagcat ttttttcagc gtcggcccgg acaatatggg
    20161 ccgccatctc tggcgactgg tgaagccaca gtttttgtgg aagatgctgc gctcaaacgc
    20221 ggcatcgctt tatggctggg atattttact ggcaggtacg gcctggccag gtaaagagat
    20281 tggtcatgcc aatgccgata tcattcgtga agcggctaaa catcacgaag tcggcctgca
    20341 cgcctgggat caccatgcct ggcaagcccg tagcggtaac tgggatcggc aaacaatgat
    20401 cgacgatatt gcacgcggtc ttcgcactct ggaagagatt atcggtcaac cggtaacctg
    20461 ttctgccgct gcgggctggc gtgccgacca gaaggtgatc gaagcaaaag aagcgttcca
    20521 tttgcgctac aacagcgatt gtcgtggggc catgccgttc cgtccactgc tcgaatcagg
    20581 aaaccctggc actgcgcaaa ttccggtgac cttacccacc tgggatgaag tgattggtcg
    20641 ggatgtgaaa gcagaagatt ttaacggttg gttactcaac cgcatcctgc gagataaagg
    20701 cacgccggtt tataccattc atgcagaagt tgaaggctgc gcttatcagc ataattttgt
    20761 ggatctcctc aaacgcgcag ctcaggaagg cgtgacattt tgccctttaa gcgaactgtt
    20821 atcagagacg ttgccgctcg gacaagttgt tcgcggaaat attgccggac gtgaaggctg
    20881 gctgggttgc caacaaattg cgggtagtcg ctgatgaaat cggtacgtta ccttatcggc
    20941 ctcttcgcgt ttattgcctg ctattacctg ttaccgatca gcacgcgtct gctctggcaa
    21001 cccgatgaaa cgcgttatgc ggaaatcagt cgagaaatgc tggcatccgg cgactggatt
    21061 gtgccccatc tgttagggct acgttatttc gaaaaaccca ttgccggata ctggattaac
    21121 agcattgggc aatggctatt tggcgcgaat aactttggtg tgcgggcagg cgttatcttt
    21181 gcgaccctgt taactgccgc gctggtgacc tggtttactc tgcgcttatg gcgcgataaa
    21241 cgtctggctc tactcgccac agtaatttat ctctcattgt ttattgtcta tgccatcggc
    21301 acttatgccg tgctcgatcc gtttattgca ttctggctgg tggcgggaat gtgcagcttc
    21361 tggctggcaa tgcaggcaca gacgtggaaa ggcaaaagcg caggattttt actgctggga
    21421 atcacctgcg gcatgggggt gatgaccaaa ggttttctcg cccttgccgt gccggtatta
    21481 agcgtgctgc catgggtagc gacgcaaaaa cgctggaaag atctctttat ttacggctgg
    21541 ctggcggtta tcagttgcgt actgacggtt ctcccctggg gactggcgat agcgcagcgg
    21601 gagcctgact tctggcacta ttttttctgg gttgagcata ttcaacgctt tgcactggat
    21661 gatgcccaac atagagctcc gttctggtac tacgtgccgg tcatcattgc cggtagcctg
    21721 ccgtggctgg gattactccc cggtgcactg tacacaggct ggaaaaaccg caagcattcc
    21781 gcaaccgtct atttgttgag ctggacgata atgccgctgc tgtttttctc cgtcgctaaa
    21841 ggtaaattgc ccacctatat tctttcctac tttgcatctc tggcaatgct gatggcgcat
    21901 tacgctttgc tggcagcaaa aaataatcct ctggcgctgc ggattaatgg ctggattaac
    21961 atcgcttttg gcttcactgg cattattgcc acatttgtgg tctccccgtg gggaccaatg
    22021 aacacgccgg tgtggcaaac cttcgagagc tataaagtct tttgtgcctg gtcgattttt
    22081 tcgctatggg catttttcgg ctggtacacc ttaacaaacg tcgaaaagac ctggtctttt
    22141 gccgcgcttt gcccgctggg gctggcgttg ctggtaggat tttcaattcc tgacagagtc
    22201 atggaaggaa aacatccgca attttttgtc gagatgacac aagaatcact tcagccaagc
    22261 cgctatattc ttactgatag cgtcggtgtt gccgcaggtc tggcatggag cctgcaacgc
    22321 gatgacatca tcatgtatcg ccagacaggt gagttgaaat acggccttaa ttatccggat
    22381 gcgaaaggga gatttgtcag cggtgatgag ttcgcaaact ggcttaatca acatcgtcag
    22441 gaggggatta ttacactcgt gctttcggtt gaccgcgatg aagatatcaa cagtctcgcc
    22501 attccgcccg cagatgccat cgatcgtcag gagcgtctgg tgctgattca gtatcgtccc
    22561 aaatgatctg gctaacatta gtctttgcca gcttgcttag cgttgccggg cagttgtgtc
    22621 agaaacaggc aacctgcttt gtggcgataa acaaacggcg caaacatatc gtgctgtggc
    22681 tgggactggc gctggcttgt cttggtcttg ccatggtgct ctggctgctg gtcttgcaga
    22741 acgtaccggt aggcattgcc tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg
    22801 ctgcagtaaa actgtggcac gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca
    22861 ttattggcgg cattgtgatc ctcgggagta cggtgtaatg ggcctgatgt ggggattatt
    22921 cagcgtcatt attgcctcag ttgcgcaatt aagcctgggt tttgcggcga gtcatctgcc
    22981 gccgatgacg cacttatggg attttatcgc ggctctgtta gcttttggcc tggatgcccg
    23041 gatactgtta ctcgggctgc tgggttatct gctttccgtt ttttgttggt ataaaacgct
    23101 acataaactt gcgctaagca aagcctatgc cttgctcagt atgagttatg tgctggtatg
    23161 gattgcttca atggtacttc ccggctggga agggactttt tcgctgaaag cactactggg
    23221 agtagcctgt attatgagcg ggttgatgct gattttcctg cccacgacaa aacaacgtta
    23281 ctgagttttc cctgccactt tacactgccg ttcccactca tcggggctat aactgcttgc
    23341 cgagaggact ttcaccgtat gcagcttttc acgattaata caataaagcg cattctgcaa
    23401 aggcgagagt aaatccccca ctttcacggc gaagtcactc ttcacttcgg cgatcatttt
    23461 tatcgcgccg ccagcatcgc agagcataag tacatgtctg ttatcttgtt tattgcagca
    23521 cgattttttg accagccatt ccattgcatt atcctgtttg ctaagagttt tcacatcaat
    23581 ttacagatta acagataaac ctattatcaa cggcaatgga atggcaaaat tgcgaagtga
    23641 aactaatgat ttcgtaatcc tggcgttgca cccactcttt taacgcctga cgtgaaattt
    23701 taataccgcc gtttttcagc tccggcggta aagttagcca gcgcaccggt tgttgaaaac
    23761 gagccagctt atctttcacc cattcgccaa gatcaacggt ttgctggtca tactccacca
    23821 ccgctaccgg tcgatgacca aactccttgt cggcaacggg gacgataaac acctgcaaaa
    23881 ccgcaggatg tgcagcaatt acgcgctcga cttcttccgg ctgaataccc tctccgccac
    23941 tgaagaacag attgtctaaa cgtccgacaa tggtcagctt gccattatgc atctcaccgc
    24001 gatcgcgcgt agcgtaccag ccttcgtcat taaccagtga aaccagttgc ccgttacgcc
    24061 agtaaccttc tgccatactg gcagcccgca gccacacttc attattaacg attttcactt
    24121 cccgacccgg cagcggcgaa ccaacgtctg ccaggccgtc ggcttctttc gcacacaccg
    24181 tggaggcaaa ctcggtcaga ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct
    24241 gttccgtcaa ctcgaccggg atagccgcgc cgccaagtaa caccgctttc agggaaacgg
    24301 aactgcggtt aaccagcaaa cgccagagtt gtgttggcac cagtgaagcg tgagtacagc
    24361 ctgccagcat ttgctccaat ggctgtttat cacgcaccgt catccgcgca ccagcgtata
    24421 accagcgcca cataattccc tgaccggaga cgtgaaacag cggtaaagag agcaaccagt
    24481 catcgtgatc gccaaacgga atcagcgata acacaccttg cgcactggca agatgggcct
    24541 gataagtatg gacagcggct ttcggcaaac cggtagaacc cgaggtcaac gtcattgagc
    24601 acagacgcgt cggctgccac gtagcggcat gtgcgccttc aacccgctga atgtgcagcg
    24661 acgttaatgc cggaaacgtg ttttccccat ccggcaccag cgcaaattgc agcgtcagat
    24721 tgggcagcaa ttcttcaagc aacggttgcg gcagctgagg gttcacgggc aacacccgcg
    24781 ccccgcattg cagtaacgcc agccaggcga gcagcgtttg cggcgtattc cacgcccgca
    24841 acatcacgcc gctgccctca accacaccct gcgccgcaaa gcccgtagct aactcatcga
    24901 cgcgagcaca aagctcgcgc cagttgagtt gctcgtcatt aagacgtaag gcgatggctt
    24961 ctccccgcac ttgccgccag tgacgccacg gccagtcaga gaagatcata acaactgctc
    25021 cagtacatca gcgtccacta acggcagtgg gctccccggc cagcgacgaa cctgctgcgc
    25081 ctgcatcaga tccagcgtgt ccagtcccgg aatggtgtct ggcgttaacc aggcagcaat
    25141 ccgcgccagt tgcgttagac ctaagctcga ctcaatggaa gaactgatca ccgccgtcag
    25201 ccccagcgca tgcgccgcct gtacctgctc gcgtactttt tccagactgc ccgtgagcgt
    25261 gggtttgata actaccgcgc gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg
    25321 caggctttca tcccaggcaa tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc
    25381 gcgggttttg cacggctctt cgagaaacgc gatgcggtgg cgataatccg ggttaacata
    25441 tttggcaaac tgctgacctt tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg
    25501 cagatccgga attgcctcca gcaacagatt aaccaccatg ccgtcgcgca cggcttcgta
    25561 caatcccact ttgaccttcg ccactttctc gcctggcata tcagcaagtt tgaggatcag
    25621 atcgtcagga tcgccattac acagtggtgc cgcacggtaa ttggccgcct gcgggagcgt
    25681 atctgccagc tccgccaatg cacagcttac gccaaaggca acggaaggca tctgcggtag
    25741 ctcgccatcg cctgccagcc agttatttac ccaagcaagc agcacaattt gcgcctcttc
    25801 ccaggtttcc tgactgaagc ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc
    25861 ttcacgcagg caaacataca gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccat
    25921 ccccgcgtcc atggggatct gccagcggta tacctgcgcg ctacgcatta cggattccgt
    25981 ttaaatttgc tgaaatcagg ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct
    26041 tcttccgtca tgtagaacag catggtggcg ttgcccgcca gctcctgcag acccgcctga
    26101 ccgtcacagt cggcgttcag cgcagctttc aggcagcgca gcgccatcgg gctgttttgc
    26161 agcatttcgc ggcaccaacg gacggtttct ttttccagat ccgccagcgg taccacggtg
    26221 ttgacaaggc ccatatccag cgcctgtttt gcatcgtact gacggcacag gaaccagatt
    26281 tcacgcgctt ttttctgtcc gacgatgcga gccatgtagg aagcgcccca gccgccgtcg
    26341 aaggaaccga ctttcgggcc agtctgaccg aagatggcat tatctgccgc gatagtcagg
    26401 tcgcacatca tgtgcagaac gtgaccaccg ccgatggagt agccagccac catcgcgaca
    26461 accggtttcg gacaggtacg gatctgacgc tggaagtcca gcacgttcag gtgatgtacg
    26521 ccggaatcat ctttatagcc gccgtaatca ccacgcactt tctggtcacc accggagcag
    26581 aacgctttat cgcctgcgcc agtcagaatg atcacgccga cgttgtcgtc ataacgcgca
    26641 tctgccagtg cctggatcat ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc
    26701 ggacgattaa tggtgatttt tgcgataccg tcggtggatt tttcataacg aatgtcctca
    26761 aaaccttcgg agcagtcgtg ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa
    26821 atcatattgt gtcctttagt cagaaacgca agatctgcgc cagacttgcg ataacgccag
    26881 cgggattttc ccgatgcgcg ttatgtccgg cgcgaggaat gacatggcag tcggcagcca
    26941 gttccgccgc caaggcgcgg aatttgctgt cacgttcacc acataaataa taaaacgcaa
    27001 atgtgcgggc gctaaggtta gcacgtaaat caggctggac ggcgagagaa gtcgcctcca
    27061 gcatggcggc aagggttgcg ccattattgt tactgcgcag cgccaccagc tcccggcgtt
    27121 gatcgtcatt gagcgaggca aaaacaggct gttgatacca gtcggcaaat accgccgtta
    27181 acggttctgt gcgaaaacgc tgcgcccatt ggcgatcgga acgctgacgt tccgcgcgtt
    27241 gttcagcatt ttgcaaccca ggatgcccgc cttcgacaac aaccccacaa agccccggca
    27301 tcccctggca agccgccatc atcgccaccc gtcccccaag cgagtacccc accaaccaga
    27361 agttaaggat gttgtaacta accagggttt tacgcagtaa gtcggtgaca tcatcaaatc
    27421 catcgacgct aatcgccgcc gaaccaccgt gacctgggag ataaacatac aaccgtgagt
    27481 agtcggcaaa cgcctcgccc acttcttgcc attcgtggca atcgccggaa aaaccgtgca
    27541 aaaacaccag ccagggtaaa cctggtttgc cgtgttttgc ctgcgcgtgc aggatcataa
    27601 atggcttacc tgcgccagaa gttgctggag cgtttgcgca ccatcggtgt cgttaaccac
    27661 catttcaatt accgtggtgg ttggcgtgcg ccaggcatcg gcaagtgccg tttcaagttc
    27721 ctgccagttt tgcggacgat gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg
    27781 gacgttttgc ggcatcagat agaaacgctc gcgctcgctt ttcggcgttg gcagcagcga
    27841 gaaaatttgc ccgccgttgt tgttcaccac aattaatatc agcggcgcgg aaacctgacg
    27901 caataacgcc agcgcgttga gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt
    27961 cggtttgccg cttgcccgct gaacgccggc ggcagtcgag agcagcccgt cgataccgct
    28021 ggccccacgg ttgctgtaca ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag
    28081 acgtaccacc aggctgttac cgacaaacaa ttgcccctgt tcaggcagat agtcgctgat
    28141 gcgatgcgcc agttgcgctt cgccaaacgc atcgcgacgg gcaataaccg cctgcattgc
    28201 ctgttccgcc aggcgcggga tttcaacgca ccagggctgg cgtttttctg ccggatgcag
    28261 ctccagccag tcggcaatat tggcaattaa gcggcgtcca cgatggtgcg ccggatcaag
    28321 ccgcccttcg atgtcatcca caatccagta ctcttcgggt tcacagcttg cctgccattg
    28381 caggagccgt ttgcccgtca ggctgcttcc cagttgcacc acaatttgcg cctgttgcag
    28441 ttcgctggtc gctttggcat tgcctaacca gagatcggca cacggcagcg gctgtccggt
    28501 ttgcgacagc acgtcgccaa tcagcggcca gccaagagtt tgcgcccaca gtgcaacttt
    28561 tttgccctct tccgcactca tgcgcccggc aaccaccacg ccgcgttttt gtcgccagaa
    28621 gaaccagtcg cgctgttttt cactttccag acgaggcgct tcacgcagcc acggtttgtc
    28681 gtcctgccac cagtcgccca gccgctgttg ccagctaagc ccggtgtcgt ccatttcgcc
    28741 atacagcggt tcggcaaacg ggcagttgat atggactcca ccagaatgaa gcgtaccgag
    28801 agcatggtcg atggtagaaa ccagccaacg tgcggggata tcctgggtcg ggcgcggcaa
    28861 tgaaatactg tgcgtggggt gagaggcgaa cattcccggc tgacgaatcg cctgattcgc
    28921 gccgcagtca atcagctccg gcgggcgatc ggcggttaac aggatcagtt tttctccggt
    28981 taacccagct tcaatcagtg ccggatagag atttgctacc gccgtaccgg aggtcacaat
    29041 caccgccacc ggctgcttgc tgactttcgc cagccccagc gccagatgcc ccaacccacg
    29101 ctcatcgaaa tgggtgtggt gaatgaatgc ggaattctcc gccgccgcta acgttaacgg
    29161 tgtagaacgc gagccagggg cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc
    29221 cagaatgacc gccgcccagc gtcggttaaa tgcgcttact gacatgagtt tgtccggtat
    29281 caatattgcg gctaagtata aggagctaca aaaatagatt attgatatga atcggtaatg
    29341 atgcgactca ttactattcc atctgtaata aagtacgcag ccctgccgct ttgttgtcga
    29401 tttcctgcca ctcttgctcg gggtcggaac cacggacaat gcccgcgcca gcatataatc
    29461 gcacgacatt gccgctaatt tttgctgagc gcagggaaac gcagaattcg ctttgctgta
    29521 atgagagata gcccgcagaa ccggcgtacc attctcgggt gaacggttcg tgacgggcga
    29581 taaactgtcg cgccagatcg cgcggtaagc cagcaactgc tgccgttggc tgcaactgat
    29641 gtaaacagat cacattatcc gctttgttga gtgaagtcca gatacagcgg cgaagatgct
    29701 gcactttacg cagacgcagt acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct
    29761 gtaaccgttg gcagatatct tccaccacca gcatattctc gcgctggttt ttatcatccg
    29821 ccatcagcca ctcacctaac tgctgcgcct gcttatcatc agggtgattt gctaccgttc
    29881 ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg ccataaccgt tccggtgaag
    29941 agccaagaaa agcattttcg ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca
    30001 gtcgacgact ggcagccatc atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag
    30061 ttgcccgagc gagcaccact ttgtcgagct caccttcggc gatggttttc gttgccagtt
    30121 cgattaattg cgtccagccc gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat
    30181 gtaacccagg caagggcttg atactcacca gtgtggcgat aaattctctt gccttaatcg
    30241 catcgtgctg aagggagctt tcgctgaata gcgtcagccg cagcgtggct ttaccgccac
    30301 agcgtcgcca ttccaggcgt ggtaaaagta aattgccctg cgacgggtca aacgcattca
    30361 gcccccaaat gcgtaagtcg gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt
    30421 ccaacgacgt aaaacgggta atcgcgccca ggacggcagc ttcttcatca ccattacgtt
    30481 gttgccagta gaattgcggg tatgtttgct gactggccag ccagctcaag gcatcaaaag
    30541 cgtctttgag agggaaagga atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt
    30601 gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag tgattgcacg cggacctctc
    30661 cctgttaaaa gcctcacatt atacggggta ctacaaaaaa aatgcagtac cccggtgtag
    30721 ggaggtttta acggcgtgcc agcaacagtc ctagtaccag cccaacggcc gcgcccacac
    30781 caattccttg ccagggtttt tcgtggacgt agtcatcagc acgataaact gcctgcttcg
    30841 cccgatagta ataactgtct gaagcctggc taacccgttt tttcacatca tccagcgctt
    30901 tttctgcacg cgctttcagc tcaacatatt tctgatcggc gggatcgcca gaggagcgga
    30961 gcacctcttc cagtgtttca ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa
    31021 actgattaga cattctcgtt ctccatttgc gtaaaacctg tcactaacta tagacaaggg
    31081 ttgttgatta cgcctgaatt acctcgcgtg ccatcccaat gtgtgggata ccatcttctt
    31141 catagacctc cgtcacaggg ataaaaccga aactctggta gaagttttgc agatgcgcct
    31201 gcgccccgag gtaaacaggc ttatcaggcc agtgatgcgt gcaggtgtcg agggttttac
    31261 tcatcagttg ctgccccact ttttcgccac gcaacgcttc actgacaatc acccgaccaa
    31321 taacgaccgg ctcaagatca tcatcacttt tcagaatcct cgcatatgcc accagttcat
    31381 catttttcca accgagaata tggcgattat cccccgtcaa atcatcacca tcgatatcct
    31441 gataagggca attctgctcg acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa
    31501 gctgggaaac cgataattca gagtgatgca gatcttacca ttcaatcata atgctctcct
    31561 tttagatgta tgccttatac tagccggaat attttttgaa acgtgatgaa ctcatggaat
    31621 taattttttt aggtacttca gccggtgtgc caacccgcac gcgcaatgtc acggcaatat
    31681 tgcttaacct gcaacatccg acccagagcg gactttggtt gtttgactgc ggtgaaggca
    31741 cccagcatca gctactgcat accgccttta accctgggaa actggacaag atttttatca
    31801 gtcaccttca tggcgatcat ctctttggtt tacccggctt gctgtgcagt cgttctatgt
    31861 caggcattat ccaaccctta acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa
    31921 ccgcgctgcg gattagcggc tcatggaccg attatccgct ggaaattgtc gaaattggcg
    31981 ctggcgaaat tctcgatgat ggcctgcgca aagtgaccgc ttatccgatg gaacacccac
    32041 tggaatgtta tggctatcgt attgaagaac atgataaacc gggtgcatta aatgcccagg
    32101 cattaaaagc tgctggcgtg ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa
    32161 caatcacgct ggaagatgga aggcagatta acggcgcaga ttacttagct gctccagtgc
    32221 caggtaaagc gctcgctatt ttcggcgata ccggcccctg cgatgccgca cttgacctgg
    32281 ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga tataaccatg gaagccaaag
    32341 ccaatagtcg cggccatagc tctacacgcc aggctgcgac actagcccgt gaggctggag
    32401 tcggcaagct aatcattacc cacgtcagct cgcgctatga tgacaaaggt tgtcaacacc
    32461 tgttacgtga atgcaggtca attttcccgg cgactgaact ggcgaatgat ttcaccgtgt
    32521 ttaacgttta actc
//
LOCUS       DAJTVJ010000038        31983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000038 DAJTVJ010000000
VERSION     DAJTVJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.038"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..727
                     /gene="istB"
                     /locus_tag="OO448_001713"
     CDS             <1..727
                     /gene="istB"
                     /locus_tag="OO448_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6336946.1"
                     /translation="ESLISAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMY
                     TRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAI
                     AMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPF
                     SQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQ
                     IKGESYRLRQKRKAGVIAEANPE"
     gene            complement(1059..2150)
                     /locus_tag="OO448_001714"
     CDS             complement(1059..2150)
                     /locus_tag="OO448_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein finQ"
                     /protein_id="HCR6336947.1"
                     /translation="MEMSQLKQPIFLKKIKKVINTIPGLEEQIFACRNKKRSDNPLLF
                     IDRKDEERILMSRLQSQQKNEELASKLESLFHGNELSSPHSILCFIYWRYTKKIYRLS
                     EDIISDVANTYVDNIPAQILKELPSWSIYVSAENLHTILPTSYPIHGFFFYPFLDNNG
                     NIIRLFIIDDLKQSQGTTGLKEKDVDVVNNIIRIKDSREGLLDSRKMECIDGEVVVTV
                     NEKLKDFRDREFNLLNAQISMVLYICSQINDIKEKNQFKRSEKHKKHVHTHHELPAQN
                     IREWDVGIRMGQAIRQYRQAEPTGKERTTIGSKRPHIRRGHWHTYWTGSKKPELAHER
                     KPRLIWLPPVPVNLEDVNKLPVVITPIDK"
     gene            2465..2617
                     /locus_tag="OO448_001715"
     CDS             2465..2617
                     /locus_tag="OO448_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR6336948.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATL
                     AYEVKR"
     gene            complement(2689..2940)
                     /locus_tag="OO448_001716"
     CDS             complement(2689..2940)
                     /locus_tag="OO448_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336949.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRGNETIPDTLH"
     gene            complement(3864..4040)
                     /locus_tag="OO448_001717"
     CDS             complement(3864..4040)
                     /locus_tag="OO448_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6336950.1"
                     /translation="MNLVDAFVKKVISGPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(4249..4458)
                     /gene="hha"
                     /locus_tag="OO448_001718"
     CDS             complement(4249..4458)
                     /gene="hha"
                     /locus_tag="OO448_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6336951.1"
                     /translation="MAKTKQEWLYQLRRCSSLKTLEKIIAKNQGTLTSDKIETFNSAV
                     DHRLAELTMNKLYDKVPASVWKYVK"
     gene            complement(4556..5170)
                     /gene="excA"
                     /locus_tag="OO448_001719"
     CDS             complement(4556..5170)
                     /gene="excA"
                     /locus_tag="OO448_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HCR6336952.1"
                     /translation="MIGAYANRGGTHSKKETCVIAFALFYIIFAVPLLIIWNTPTSWG
                     LAVIPTGFLLYSGYKNGRKKRAIVNNILEQIKTEYHDVFDPDPSYEHKSISSLYFGID
                     IKKGTALYIRLYPNKTLDVIGIDIDNFTRTVVRENCMEIHTKYVNMPMLELPIGVNSA
                     RSIANTLHAMASRGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(5246..7414)
                     /gene="traY"
                     /locus_tag="OO448_001720"
     CDS             complement(5246..7414)
                     /gene="traY"
                     /locus_tag="OO448_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer membrane protein
                     TraY"
                     /protein_id="HCR6336953.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDVVGTLSVVAGFLMIVPTGNGWSLAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGTASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPPLGTITSADEQLLSKTSTPQDALIKPTEKFGQWLTNNIATEWTDSGTS
                     SNQVNPLIKMKNIGDKTMVAAEGIWITYTSARVITSGTGSSIFGKFIDLTTSAISTLN
                     ALLEALAPPVYFLLLLLFCAGFSLSIYLPFIPFIFWMTGIGNWVISVLIGCTAGPLWG
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAIGTILNFLFGAALT
                     NVQISSFTGIFSLAGFLLLYARSCTTIVAAAFALQAYLPDHVINFLGGRDGVNTLGNM
                     AGSIKEIFAGSNRNIRHAPNIREEQIKRMNNDNNKDGIKG"
     gene            complement(7511..8095)
                     /gene="traX"
                     /locus_tag="OO448_001721"
     CDS             complement(7511..8095)
                     /gene="traX"
                     /locus_tag="OO448_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HCR6336954.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            complement(8124..9326)
                     /gene="traW"
                     /locus_tag="OO448_001722"
     CDS             complement(8124..9326)
                     /gene="traW"
                     /locus_tag="OO448_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HCR6336955.1"
                     /translation="MQRKTLLAALIAILSGTACQAHAYSVTVVASRPVEQQVVPRMEA
                     IKGVLSDILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRNIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NETTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            complement(9293..9907)
                     /gene="traV"
                     /locus_tag="OO448_001723"
     CDS             complement(9293..9907)
                     /gene="traV"
                     /locus_tag="OO448_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HCR6336956.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRLFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            complement(9907..12951)
                     /gene="traU"
                     /locus_tag="OO448_001724"
     CDS             complement(9907..12951)
                     /gene="traU"
                     /locus_tag="OO448_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HCR6336957.1"
                     /translation="MNINRFIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISCVFERDPEMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERYSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICARNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLIRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEDLASELIRKQGYNL"
     gene            complement(13041..13841)
                     /gene="traT"
                     /locus_tag="OO448_001725"
     CDS             complement(13041..13841)
                     /gene="traT"
                     /locus_tag="OO448_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HCR6336958.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            complement(13825..14013)
                     /gene="traS"
                     /locus_tag="OO448_001726"
     CDS             complement(13825..14013)
                     /gene="traS"
                     /locus_tag="OO448_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HCR6336959.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            complement(14077..14481)
                     /gene="traR"
                     /locus_tag="OO448_001727"
     CDS             complement(14077..14481)
                     /gene="traR"
                     /locus_tag="OO448_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HCR6336960.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLASC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            complement(14532..15059)
                     /gene="traQ"
                     /locus_tag="OO448_001728"
     CDS             complement(14532..15059)
                     /gene="traQ"
                     /locus_tag="OO448_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HCR6336961.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            complement(15059..15763)
                     /gene="traP"
                     /locus_tag="OO448_001729"
     CDS             complement(15059..15763)
                     /gene="traP"
                     /locus_tag="OO448_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HCR6336962.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHTAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            complement(15763..17052)
                     /gene="traO"
                     /locus_tag="OO448_001730"
     CDS             complement(15763..17052)
                     /gene="traO"
                     /locus_tag="OO448_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HCR6336963.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTQ
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALKNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            complement(17055..18038)
                     /gene="traN"
                     /locus_tag="OO448_001731"
     CDS             complement(17055..18038)
                     /gene="traN"
                     /locus_tag="OO448_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HCR6336964.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            complement(18049..18741)
                     /locus_tag="OO448_001732"
     CDS             complement(18049..18741)
                     /locus_tag="OO448_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HCR6336965.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            complement(18738..19085)
                     /locus_tag="OO448_001733"
     CDS             complement(18738..19085)
                     /locus_tag="OO448_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR6336966.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            complement(19103..22870)
                     /locus_tag="OO448_001734"
     CDS             complement(19103..22870)
                     /locus_tag="OO448_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6336967.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAATPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTDTVE
                     PAVRDDPTPQQPVQSTSSSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSPETTTTT
                     PAPDSIVYAPERPDVPISLNLDEIIKSLEGEERADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATDFYGGVIELTGSDAFKQKAMQLIIEHNIKVRMKFPDQRAA
                     LEKLRKEMAVGKDTVVTHKPTPELNRNTPEQPAVPDPVKEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            complement(22960..23511)
                     /locus_tag="OO448_001735"
     CDS             complement(22960..23511)
                     /locus_tag="OO448_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HCR6336968.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
     gene            complement(23526..23816)
                     /locus_tag="OO448_001736"
     CDS             complement(23526..23816)
                     /locus_tag="OO448_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR6336969.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            complement(23813..24961)
                     /gene="traJ"
                     /locus_tag="OO448_001737"
     CDS             complement(23813..24961)
                     /gene="traJ"
                     /locus_tag="OO448_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HCR6336970.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            complement(24958..25776)
                     /gene="traI"
                     /locus_tag="OO448_001738"
     CDS             complement(24958..25776)
                     /gene="traI"
                     /locus_tag="OO448_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HCR6336971.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            complement(25773..25895)
                     /gene="traH"
                     /locus_tag="OO448_001739"
                     /pseudo
     CDS             complement(25773..25895)
                     /gene="traH"
                     /locus_tag="OO448_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
     gene            25967..27144
                     /locus_tag="OO448_001740"
     CDS             join(25967..26225,26225..27144)
                     /locus_tag="OO448_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6336972.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVANIT
                     "
     gene            complement(27151..27492)
                     /gene="traH"
                     /locus_tag="OO448_001741"
                     /pseudo
     CDS             complement(27151..27492)
                     /gene="traH"
                     /locus_tag="OO448_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
     gene            complement(27887..28471)
                     /locus_tag="OO448_001742"
     CDS             complement(27887..28471)
                     /locus_tag="OO448_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6336973.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            complement(28531..29733)
                     /locus_tag="OO448_001743"
     CDS             complement(28531..29733)
                     /locus_tag="OO448_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HCR6336974.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            complement(29819..30643)
                     /locus_tag="OO448_001744"
     CDS             complement(29819..30643)
                     /locus_tag="OO448_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR6336975.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTAGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINDSSNTEVIFWHSFPFAGNTKREYYGVILRQKKEINGSVIYPALSLS
                     PTILNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            complement(30794..31948)
                     /locus_tag="OO448_001745"
     CDS             complement(30794..31948)
                     /locus_tag="OO448_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6336976.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTINEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
BASE COUNT         7584 a         7630 c         8031 g         8738 t
ORIGIN      
        1 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
       61 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
      121 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
      181 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
      241 catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct
      301 ggcaatagcg atgggctatg aagcagtccg tgcaggtatc aaagttcgct tcacaacagc
      361 agcagatctg ttacttcagt tatctacggc acaacgtcag ggccgttata aaacgacgct
      421 tcagcgtgga gtaatggccc cccgcctgct catcattgat gaaataggct atctgccgtt
      481 cagtcaggaa gaagcaaagc tgttcttcca ggtcatcgct aaacgttacg aaaagagcgc
      541 aatgatcctg acatccaatc tgccgttcgg gcagtgggat caaacgttcg ccggtgatgc
      601 agcactgacc tcagcgatgc tggaccgtat cttacaccac tcacatgtcg ttcaaatcaa
      661 aggagaaagc tatcgactca gacagaaacg aaaggccggg gttatagcag aagctaatcc
      721 tgagtaaaac ggtggatcaa tattgggccg ttggtggaga tataagtgga tcacttttca
      781 tccgtcgttg acattaaaaa tttattttca ggtgataaga gctaaccaga ggagttagcc
      841 gtgcgataaa aggattgttg cagatacgaa agtcgttaga atagctgcgg gcgcttgagg
      901 ctgtatgccg aaagcgtttt gtggacggta tacagcagaa agcccctgga gattttttta
      961 tcaatcaacc aagggctcta ctgtaatgcc tcaacaacat tatagtagcc tgataattta
     1021 gatatgtgta catcctaccg gaaaagatac ttttcaggtc atttatcaat gggcgttatt
     1081 accaccggta gtttgtttac gtcttctaaa ttaactggta ccggaggtaa ccatattaat
     1141 ctaggtttac gctcatgggc tagttctggt ttcttacttc ctgtccagta tgtatgccag
     1201 tgtccccggc gtatatgagg acgtttgcta cctattgttg tccgctcttt ccctgtgggt
     1261 tctgcttggc gatattgccg tatggcttgc cccattcgaa taccaacgtc ccactcccga
     1321 atgttttgtg ctggtagttc atggtgagtg tgaacatgct ttttgtgctt ttctgaccgt
     1381 ttaaattgat ttttttcttt tatatcattg atctgtgaac agatataaag caccattgat
     1441 atctgtgcgt taagaagatt aaactccctg tctctgaaat ctttgagctt ttcgttaact
     1501 gttacgacga cttcaccatc gatacactcc atctttctgg agtcaagaag tccttcacga
     1561 gagtccttta ttctgatgat attattgacg acgtcaacat ccttttcttt tagccctgta
     1621 gttccttggc tttgtttcag atcatcaata atgaaaagcc ggataatatt tccattattg
     1681 tccaaaaatg gataaaagaa aaatccatgt atcggatagg acgtaggaag tatagtatgc
     1741 agattttcgg ctgaaacata gatactccat gaagggagtt ccttaaggat ctgtgcagga
     1801 atgttatcaa cataggtgtt agccacatca ctgatgatgt cctcgcttag gcgataaatt
     1861 ttctttgtat agcgccagta tataaaacac aaaatactgt gtggtgacga taattcgttt
     1921 ccatgaaata aagattctag tttagaagcc agttcttcat ttttttgttg gctctggaga
     1981 cgactcatta gtatgcgttc ttcatctttt ctgtcgataa ataataatgg attgtcgctg
     2041 cgttttttat ttcggcatgc aaagatttgc tcttcaagtc cagggattgt gttgatgact
     2101 ttttttatct ttttcaggaa aataggttgt tttaattgcg acatttccat ctcctaaggg
     2161 gattctttta ttaactaaaa tcagcataac atgcgttgtt gtgaagtaga gaaccatgta
     2221 ttgggttggt tgttaacgtc aaaaacaacg ctgaatcatt caaataataa aaggattgtt
     2281 acagatacga aagtcgttag aatagctgcg ggcgcttgag gctgtatgcc gaaagcgttt
     2341 tgtggacggt atacagcaga aagcccctgg agattttttt atcaatcaac caagggctct
     2401 actgtaatgc ttcgacaaca ttatagtagc ccgataaccg ccgtaaggca atggaggggc
     2461 tatgatgcca cagcgaacgt ttttaatgat gttaatcgtc atctgtgtga cgattctgtg
     2521 ttttgtctgg atggtgaggg attcgctttg cgtactccgg ctccagcagg gaaacacagt
     2581 gcttgtggca acgttagcct acgaagttaa acgttaacgg gcaacacggc ggcaggtttt
     2641 ctgccgccgc tttgttgcga aggctgacct cattgcgccc tttcttcttc aatgaagcgt
     2701 atccggtatt gtttcatttc ccctgaagcc gattagctcc agccgaatgc ctttgccatc
     2761 gggggccacg ttctgcattt cgcagatacc acgtcgggtc atgagtgtga aggctgtcat
     2821 tgccagcatg gctaacgcgt tttcctcgct tccgctgcgt ttctcgagag catcggcaat
     2881 ttcttctatg ctccccacct gaaagcaaga gtcgaattct ttactccact tttctgacat
     2941 cggattttct ctctgtattt atgggtagtt atcgaactga atgtgccagt tttttcagaa
     3001 gacggcaaag ttgagatggc tggttcaggt tcagagcatg agtttccccg gtgtgacggg
     3061 catggcgggg cggattttgc agatacaaaa gacgtaaaaa aaccgactgg tgaggccggc
     3121 ttttttactg ttacgctaca aagttgatga ctctgaccag aaaagagctg tctataactg
     3181 acgctaaaaa tgataatttg ttctacctga ttaatgcaac atgccataag tttacgggaa
     3241 aggctgacca cctgatattg ttgcaaaata cgaaagtcgt caaaatcgcg gtggggagct
     3301 tgaggctgta agctgaaagc gtttgtgaaa gccccgggag attgttctat caatcaacca
     3361 agggctctac tgtaatgcct agacaacatt atagtagccc gctgacgtcc tccacgccag
     3421 agggagcttg ctacgtacag cttgtcgtta ctcagaagta ataaatgtaa aaaaaccgac
     3481 tgatgaggtc ggttttctta ctgttccgtt acagagttgg tggccctgac tggaggaaat
     3541 ctcttttcag ctaacgttct tataatagtg ttctgttcat ttgataacaa ccacccttgt
     3601 aatccgcagg ttagcactgt ctggcgacac ttctgtcgtg atgacggtaa ccagaacatg
     3661 agtcttcccg gtataacagg catggtgggg cggatttgca gaaacaacag atgtaaaaaa
     3721 accgactggt gaggtcggct tttttactgt tccgctacag agttggttgc tctgggcggg
     3781 aaaagacagt ctgtatctaa cataatcaat tctatgcttt gtcccacact atgtcaaaaa
     3841 tcctgctaat caaaaaagag atctcacgtt aaaaatttgt acccttcttt aacttccagt
     3901 gcctgctcct ttgactcaaa catcagtctt gttttcccgg gaacacccca tgaaatgtat
     3961 tcaacatcta tccaccattt tccatattct tcatatggcc cggatattac ttttttgaca
     4021 aaggcgtcta ccaggttcat tgttttttcc tttttgtgtt gaaaataatt aatgccagag
     4081 cacagcgaaa ataatgaatg cgctggctgt gataattgcg attttctccc atatgatatt
     4141 tttctcatgg gtgtttctac ggagattatt tcttcgcatt ggaatatatt ggatatgttt
     4201 attcaggaat gagagcaggt gtgggattcc ctgctctttt tgttggcgtt attttacgta
     4261 cttccagaca gaagccggta ctttgtcgta caatttgttc attgtcagtt ccgcaaggcg
     4321 atgatcaact gcagagttga atgtttctat tttatctgag gtcaaagtgc cctggttttt
     4381 agcaataatt ttctcaagtg tctttaagct ggaacagcga cgtaactgat ataaccattc
     4441 ctgttttgtt tttgccataa ataggtcctt gggtttattt aatacggcat aagctatgtg
     4501 ttgttgcaaa caaaagccgg ctcatctgaa ccggcagtta tggaggctgc ttatcttaaa
     4561 atacttcagc tacaggcata cctgcaatct gctcccactc cttacgcttt tcctgaatca
     4621 ggcgaggaaa gtcgacaggg taatcatatc ctcgacttgc cattgcgtgc aatgtgtttg
     4681 caatagaacg agcagagtta acaccaatag gcaattctaa cattggcata ttgacatact
     4741 ttgtgtgtat ttccatacag ttttctctta ctactgtccg tgtaaaatta tcaatatcaa
     4801 tgccaatgac atctaatgtt ttattggggt aaagccgaat atataacgca gtaccttttt
     4861 tgatatcaat tccgaaatat aatgatgata ttgatttgtg ttcgtaagat gggtctggat
     4921 cgaatacatc atgatattca gtctttatct gttctaggat attatttacg attgctcttt
     4981 tctttcttcc atttttatat ccggagtata gcaggaatcc agttgggatt actgctagtc
     5041 cccaactggt aggtgtattc caaatgatta gtaatgggac ggcgaaaata atgtagaaaa
     5101 gtgcgaatgc gatcacgcat gtttcttttt tagaatgggt gccgccacgg tttgcatatg
     5161 ctccaatcat tatatatgac ctccataaac actttttgat attttaatct ctccaagtgt
     5221 atatggcaag tcatatatta agttattatc cctttattcc atccttattg ttgtcgttat
     5281 tcatcctttt aatttgctcc tctcttatat taggtgcgtg tcttatgttt ctattgctgc
     5341 cagcaaaaat ctcttttatt gaaccagcca tattgccaag cgtgtttaca ccatcacgtc
     5401 ctcctaggaa attaattaca tggtctggga ggtatgcttg taaggcaaat gctgcggcta
     5461 caatggttgt gcaacttcta gcataaagaa gtaagaaacc tgcgaggcta aatatacctg
     5521 taaatgaact tatttgtaca ttggtcagtg cagcaccgaa taaaaagttt aatatagtac
     5581 ctattgctat aatggctaca gaggcaaaaa agaatccgaa aaccatgata ggtggtctta
     5641 tcatgctgtc tattaaatat atataaccgt aggcagcacg actccctctg tcctgtgatg
     5701 tgcctagatg agttgccccc cacagaggtc cagctgtaca tccgattaat acactaatga
     5761 cccagttacc aatcccagtc atccagaaaa taaacggaat aaatggtaaa taaatagata
     5821 aagagaatcc tgcacaaaac agcagtagta acagaaagta tacggggggc gctaaggcct
     5881 caagtaaggc atttagtgtt gaaatagcac ttgtcgtcaa atctatgaat ttaccaaata
     5941 tagaactccc agttcctgaa gtaataactc tagcagatgt ataagtaatc caaataccct
     6001 ctgcagcgac catagtttta tcaccaatat ttttcatttt tatcagtgga ttaacttgat
     6061 tgctacttgt acccgaatca gtccactcgg tggcaatatt gttcgtaagc cattgcccaa
     6121 acttctctgt gggcttgatt aaagcatctt gtggcgtgct tgtttttgaa agtagttgtt
     6181 cgtctgctga agttatagta cctaaaggtg gggtataagt tgaattctgc aattgagtct
     6241 tgtatgcccc cataacagca ctaaataaat cagtatcgcc aacttcccca agtgatgaca
     6301 ttgaagttac agctggagct ctgtcagcca gctctgccag acgctggtta gcagtggcaa
     6361 aggtctgata ccacgcgcca agcgttaccc atccataggt tgtgagataa gacttgagag
     6421 cttctttcct ggattgttct ccgttatcaa taggtagcga tttttgtaca gcacgctcat
     6481 actcgtcggc tgctctctga atccgtgtct cgatatccgg caatgtgccg ttgccagaat
     6541 ttcttttttc cagaaatgtt gtcacaaact cactggcagc gttgtccatg tcgctaatca
     6601 tattatccat ggctgcacgt tgtgctgaga taacaccgct gtattcgttt ttactgaagg
     6661 gattaaagaa ttttccaatc gtcgattgat ctgttgttcc gtttccttct actgacaggc
     6721 tggctgtacc acagataccg ctgccattgg ataccgtgac agtgtaatta ccactggcgg
     6781 ttttagctga ttctgtcatc agtgaagtcg aggatttggc cgtctgatta aaatcattta
     6841 gtcctgcgtt aaccgcatac ttacacagtt ccatttcaaa aatgccacgc gctgctgttc
     6901 gtgtggatgc ctgaactggc tgtacagtca ttgaataacc atttgcgata ttatcagcgg
     6961 caagttgaac catcacattg gcggacccaa cccccattat cgatgcgccc cataacataa
     7021 tgagctgagc gagactccag ccatttccgg ttggaacgat catcagaaaa cctgcaacga
     7081 cactcagtgt gccgacgaca tcgcgtccgg tgctgaatac ctgtccctga tgccccgaac
     7141 gcacaacatg acggatacca atgaacataa accagacaac ggcaagtacg gcgatgatac
     7201 tgttgaacgc cccgaacatg cttccgatta gggtgggggc gctggttgac agtggattgg
     7261 tgaccacatc accgaatatc gtgaccagcg cctgacggga cagatcgtca ggattggttg
     7321 cggcagacac aatatcctga taggtcactg aagccattgc cggcagtgag gatatagcaa
     7381 ggcctgcaca gagtgcgcga agtaaaattt tcacagtaac tccggaatag aattgcgtat
     7441 gggcaaaggc cacacaggat ttaattaagg gggatggcga catgccagtg gtattggcat
     7501 ctggacgtga tcagtattgt ttactggtta cggcaattaa cgtggcattg cgccagccat
     7561 tgcggtcgtt cagaaaatca cggaaagtcc cctgctcggg ttcgctgact tttcgttcat
     7621 gcaactgcca gaggcggtat gtaacaatca gtgctctgga agcacaaaga gcgacgcctg
     7681 acagtatcag cacaagagtg ctgatggcgc gtaccagagt cgtaagaggt aatgaagagg
     7741 cggcaaagag cataccactg agaaacaaag cgatggccag cagactccag caacagaatc
     7801 gccatactgt tctgcgccgg cggaaacgac gatccagatc gtctactgac atatggctgg
     7861 cctgcagagc ttcttcccac gtgaggtgga gttgcgcctt tttattattt acctgacgaa
     7921 atctgtccag tctgcgtttt tgttgttcca gcatgtgctg tacagaattc gttatcaggc
     7981 gagtctccat cagaggaaca aaaatgttaa taattccccg ggtggctatg cgggttatgg
     8041 atggtgattt tttgacgttt ttcgtctggt ctgaattttc cggtaactta ttcatttctt
     8101 cagtcctgtt ctgacgtcag ctttcaacgt gcgtttgcct gattgattgt tttctcaagg
     8161 ctaccgagtt gtttttcata atactggcgg gcggtcagag ccagttgctg tccagagatg
     8221 acattaccct ggcggatctg ttctttcagg tcgttcagct gccagttcat ttgcgcagtg
     8281 attcttacca gttcacggag caggttatct ccctgcattt cctgtaaatc caccaggtat
     8341 gctgtgttgg cgtagcgccg gccagcctca aaagcttcaa actcacgggt actcatatat
     8401 ccccgtgctt ttgcttctgg tgaagccacc tggtcgaagt aagcggccgc tgagtctgac
     8461 tgcagtgttt cagccagtgc tttacgtgtg gtttcattag gcgtgctgtc agcaatcagc
     8521 gtcagttggg gattcgacgc agaatcaatg atgccgttgt attcgttctg gagtccgacg
     8581 taggttcgcc cactctgggt attcacttct cctttaccca gagcacgacc ggcagagggg
     8641 cgggaagtgt ttttcatgta agccgtcgct gcatctattt gcttctgatc ccatgtcagt
     8701 gccgcaggtg tgtccgctgt acctgcgccg tgataaatcg agcgtacctg actgtctcca
     8761 cctggtattt cgcccacaga aggacaaacg gcggttcctc caaaacgtgc atattctgct
     8821 tctgtacagt aactggcgtg tatggccgcg ccatcataag cttcccgcac gggagagttt
     8881 gctgcgctgg ccagacggtc gcggatgtta cggttgctca caccgccacc tttactgagt
     8941 tttgaggctg cggatgctga ggcacttttg ctttctgttg caatccctga tgcggattca
     9001 ctgcagatgg agtctggcac ggtgaagctt ttccgtgcct cctcgaggcg ctgagtttca
     9061 ttactgaaaa tcatctgctg acgtgtcgcc tgtccgttct gggcaatgac acttgccagc
     9121 ttttcagagt tctgattgat agcggtaccc gtggctgtct gggtactcag tatgtcactc
     9181 agcacacctt tgatggcctc cattctgggg acgacctgct gttcaacggg gcggctggcg
     9241 acgacggtga ctgaatacgc atgagcctgg catgccgttc cactcaggat ggctataagg
     9301 gctgcgagta atgtttttct ttgcatttta ttgtctcctg ctcggggtgg agcacaaggt
     9361 caggaaggct gtcgtatttt ctgtcttcag gtagtggggc cagtggaaaa cactgatatc
     9421 gcctggtgat ataacctttc ctgttccggt tatcgatgat gctgaaagaa cgacgtctga
     9481 cgcgccgtaa ggtggacggg tcagtatcca tgacaggcca gtcaccggca ctcagtgccg
     9541 gcgttccggg aacggatggc cataaccagg tatcatcgga catcagcgaa gccagcggtg
     9601 aagggagtat ccactggatg acatgctgaa cgtcgaatgg tgcttcatcc gggacagaaa
     9661 cctgccatat tgccgttgtt atcagctcat ctgtgctgga cggcaacaac agccgcatgc
     9721 ctgtaacctt tccctgtgtt ctggcatccg aggcgtatat cactctggga ttgggggccg
     9781 gatgtaacct gaagagacgt gacggcagag tgaatgagaa actgtcacct tttacctcaa
     9841 gttttttaag aaatacctgc cctctggcag gtctccggca gcgtggggtg atatgaattg
     9901 tacacatcag agattgtatc cctgttttcg gatgagctcc gaggccagat cttcaataac
     9961 attgtcactg ttatgttggc ccgcccggtg ctcaattaat gacgttgcag aacctctggg
    10021 gaagttttcg gccagaatct tacgtgcccg aactgaacct aacttatttg tcagcgtttt
    10081 acgcagggca ctgtcttttg gcgatgagtt cagcgcccat agctccagtg gaccgacggt
    10141 aaatttgagt attctcgcaa gcgtgccact ttttaccctg aatactccca gaacaggaac
    10201 accactacca tctggtgccg ggccttccgg cattttcagg aagcgtttca gcataaattc
    10261 aggcacgttg aagttatccc tcagtacggg gatatcttcg ggtttgtagc gtattaacca
    10321 gagagtattt gctgacttaa gaacgctttc cgggtagtcc ctcagatact gcgtactcag
    10381 aaccgtgcgg atagcaaact tgcgttgttc gcgctcctgg gtatccagat tttcccagat
    10441 gaagtcaatt ccccgggcgt tatgcagttc atcataaact tttgtcttaa cctcctgatc
    10501 aagttggtta atacgtttaa gagcgatttc atggtattcc cgtggaagct gctttaacac
    10561 ttcatccctg tattgtggca gggtgaagtc accaccggcg atctgacctg caagcaggta
    10621 catgatgccg gttttaagcc tgcctgcagg cgttttgtca ccggcaacat tattcagatc
    10681 gacagcgatg acccgggttt ccgggttgat cataaaccgg gtatacccgg atatcatggg
    10741 gtagtcgtta tgtccctgct ccagggcgcg gcggatgtag tccagcagaa gctcgtttga
    10801 accatcacgc tgaacagtac cgaaaacatc cctgagactg gtatggccca gcatcgagga
    10861 cacttcggga agctggggca ttgcctgata atgagcccgc tgagccgcca tgatataccc
    10921 ggcattgtgg agcatatccc ggacttcgaa ccaggttgat ctggcccacc aggctgcatc
    10981 atgtttttca tacagtcccg agtcctgcag tgctgaatcc accagttctt ccgttgaagc
    11041 gcgataaagt cgcggattat tttcaccgta ctctttaaaa gccagctcga taagctggtt
    11101 gataatttgt cgggtgtctc cggggttaca gggttgaccg gtaccggtat cgacacagag
    11161 agcacacagc accgatgaca tgaaattctt ttccggcgta ataggctttt tagcgccgta
    11221 catcacgtca aaaaggttac gggtgtattc cggatcattg ctcagaataa taccgacagc
    11281 ttcgtctttt ctttccggag gaagactgtc gcgaataagc tgaaccagtc cctgagcact
    11341 gtatccctta tcgatatagg caatgaaggg caggtttttc tgagcagatg atatctgtat
    11401 ttccgacaac gtattaatca gaactgattt acccagtccc ggatcgcctg gtgccagttc
    11461 tgtatgttta ttttgttggg atgacgccag accaacctca aatgcggaac cgtcttctgt
    11521 atgtaacatc agattccctt tgcctcgcca gacagagcct gccctgttaa gcgggaaaag
    11581 cgagatggca tgtgacaacg ggggatacag gggaaccggg ccggagcctc cggacgccgc
    11641 gagaatggta ttcacccaag cgcgtcgggg atcgccaaat gttgtcgtgg tgccacatac
    11701 gccccagcct tcaatggccg atttcagaat ggcctggttt cgtgcgcata tttcacgtgt
    11761 ttttccccac gtggaggcca tgatggtcat tatgcagaca ggttcttttt catccgttgc
    11821 cgccagagtc ataacggatt catacattgg acgaacggca gaaatgaagc tgctgtaagt
    11881 cagaagcgtt ttcttcagat taagagcctt catcccgccg ggcatcagat ccattctgat
    11941 gcgccagggg actgcgcggg ggacggcacg gaccagttcg ttaaacgtct gcagattttg
    12001 cggaggtaaa gtaatcgata ccattccgtg ccagagtccg cccgcctgaa ccaggttgcc
    12061 ctgtgtggtc acctgcgtgt tgaataactg caaatgcaat gaaggagcat gtagtacgga
    12121 ttcatcgtca gtccagcgat atcccgctgg ctgggcatct tcaggcaggt ggggttgcca
    12181 gttacgcggt gtgctgtacc gttctgtctg acgacgtatt tcacgcccga cctcgtgtat
    12241 atccagtagc cggagaatca ggccatcaga tgaatgtctc agtgcctgtt ccacattatc
    12301 aagaaaagct tcgtggcgga ttttcagtgc agataaggtc cactgccagg gggactgggc
    12361 aaagcgtgct ttcggtaccc gttcagccag tcttctgaca agctcatcat gggcggtgcg
    12421 atcgctgttg ctgataaggt cgggtccact ccagatcgcc agccagcagc gctcccggac
    12481 cagccatggg gacagtgttg tgactttctc atcgaccaca tcctgtaact ggatacctgt
    12541 attagccagt gagcgttttt gtggggcgac catgtcttcg atttcttctt tccccatttc
    12601 agggtcacgc tcgaaaacgc agctgatttt atgaccgctg tttttatatg ccgtattcag
    12661 gctgtcactc atgctggtga tcagcgattc cagtgaacct gggccagtct ggtcggatgc
    12721 ttcatccatt tccctgaaag caccgctgac ttcaaagaca gagagatatt ctccacgctt
    12781 tgtggctgca atatagggcg aattaagcca tgggcgacgt tcacgatcct gtctgtccag
    12841 accaattaca gtacgtaaat cgcaatactc aacaaaactg cttgcgacag agaaacggga
    12901 tagcgtgttc agacagtctt cgatagtgaa gatgaaacgg tttatgttca taggtgaggg
    12961 aactccggac ttacaggagg gaggtttgtg cagtttgatt tttttaatgc caccccgcgc
    13021 tggagtggca tgaagaaaaa tcaatcaacg tattcttttt tgatgaatga atctggtgtc
    13081 agaatcaggg caatcccgtt cattttttca cgcaggggcg aacgaccaga aggtggatag
    13141 cgacgaaaaa cgtcagcgaa atcggccggg cggaaactgc ggaatttatc ctcaaccgcc
    13201 agtctgtttt gcatcagtgc agagagcgct tcccgtcccc gccggacccg gaacttatca
    13261 ttactgaaaa cttcctgtaa tgaacgacgg ttaagggcca cgaccgtgga tggatgaagg
    13321 tcgggtagcc acaccataac gccctgtgct gcggcataac cattcagtga atgccaggcg
    13381 tggcagaaag cacataagct gtaaggcggg ccgtcttcat gaaacagttc ctgctctttc
    13441 tctgacaacg actctgtact ggtgatttca gctttctgta catggcggag tgaaagacgt
    13501 ctggcagaca gacgggcctg ctcaagcgcc cgttcagcgg cttttactgc cgtgttgtac
    13561 ttttcctttt cgccggttgc gactggttca ccacgctttt ttaacttatc cagatgttct
    13621 gtggctttat taagacgaac tcgtgtgtca ctgattgtgt cggtaagctg tctgagtggc
    13681 catgagtcat ccagtctgaa acggtgcctg acgttttgcc ggcagcagat acatcgaccg
    13741 ggcgttgtta attccggttc cggtaacagt ggctcctgtg tatgaaccac atcaatttct
    13801 ggaaagggat acaaccacaa gtctttaatg ctgtgttgca tgagcaggct ccgtactggt
    13861 gtaacgtttc acggcttcca gaatgatgtt tgcgatttca tcggaacagc attctgcaaa
    13921 aatgaattcc tgatcgtccc gactgtcgtg ggcaatcaca ttgccgtttt ctgttcgtcg
    13981 taacgttatg tgctccgggc tccaggcaga catatgtgcc tccttgtatt gccgaatagg
    14041 aagggcaccg gataaatgcc agtgccctgg acaagattag ccaatggaaa ttgaggaaat
    14101 ccccaactgt ttctggctgc gactcatcat ttcaggtccg gcgacaagta ccgccccaat
    14161 gacgatagat acaatgcaac tagccagtcc catcccccct tgtttcccaa cttttttcag
    14221 gccaatcagg cccccgagaa acagaattac gccgatgaac tgcgcaatag tcagtgatga
    14281 tgactgtgct gttttggcac cttgttcgac attgcgaacc atatcggcaa gatctccatc
    14341 ggcaagaacg gctttgggca gcaacatcat gggaatgagc agcaggaact tagtcagttt
    14401 gctccggcat gacattacaa acagttgtgc gtggataaat gcggcgtaca gacgggccgc
    14461 tacgacacga atacgtgaca tagtttctcc ttaaatggat atatcagtgg tttatatgaa
    14521 ggtgaaaaaa atcagaagac gattccaatg cttgccagca gggcattgag gacacgatcg
    14581 ataaagacca gcaggacgcc ggcaataaat ttgacgtttc ccttactgac ggattcactg
    14641 gcactcagtg cggtatgtcc atcgagtccg gccttgcgcc agatggatat cccgttcatt
    14701 acaaatccaa caccggccag cttggccagc gacagaactg catttgctgc gccagcagca
    14761 gcgccaaacg tctgaggttg tacatatgca atcgggccaa atgagtcagc cctgaatccc
    14821 atttgtacgc ctccggcgtt aatcattgcc gggagtgata cgagcatgca gctggttaac
    14881 atccccgcaa taaattttcc gggagacatt tttaccggac cggctgaacg ggcgcgggca
    14941 aacagggtaa tcatgctgat caccccgatg aattctcccc aggtaaacag gaaatccatt
    15001 ccggcactga aaatactgct ggcaaagttc tcaatggcgg tcagggcatc catgttcatc
    15061 agcgtaacgt ccctgcactg gtgatgatgg tccgggtagt cggatcaatc cgctggatca
    15121 ccacattgcc cagcgtctga ccttctctga cagcccaggt actaccctgc cagcgaatcc
    15181 aggccatacc agtttccatc gacatgattt tcattccgtt aagtacagac gacgtttttt
    15241 tctttgctgt atgcggtgtg gaagaggatt cagtgacgct tttctgtgtc attattccgt
    15301 tcaggcggct ccggatatca tccagctgac ggcgtgtttc ctgtgcgtat tcttcatcct
    15361 tttttatagc ttcgcccagc ttgttaatgc tgtcgtgaag catattcagt gttgcctgaa
    15421 ttttttcatc gcggttatcc agctctgttt ttacatcccg ggcaagctgg ctcatatctt
    15481 ctcctgtccg gacagtcata gtctcagtgg actgaacgga aggtgatgat tcttgtgtgg
    15541 tttgccaggt ctgtacgcca gcatccgcat cattaaacgg aggcatgcct gtttccgatg
    15601 gcgctctcag gaacagatac catatggctg cgagcaacac gactgcacat agcccccatg
    15661 ttgctacaga tatcccccag acggaacgtt tccagaaggg ggcgggtttc tcttcgggtt
    15721 caccgaagct gccggtttca tctatttctg tttcaggttt cattatggtg tctccggttg
    15781 ttcatcatac cgacgttgca tcacagcttt tctttgctcc atggctgtct gcattcgtgc
    15841 ctgcgagcgt tgttccgcct gtgatgtacg attaactgaa ggggaaatgc cactggaaag
    15901 ggaagatgac ctggcgtcgt ttgtcattac cgggtcaaca aacagaattg atacgacttc
    15961 atgctgatac acttctacct gtttataggg ctcactttct gcctggcggg ttaatatctg
    16021 acttcctctt tccgccatac caccggcaat aacgcccgca acagcttcac cactgggcat
    16081 tcccacgcgt ccggtaaccg tgccatagtt accctgaata acctgcgtgt tggcatcttt
    16141 ataaagtgtg ccaatactgc ccacacctcc taacactgac ggcagaatga tatgtttaaa
    16201 ccagcgtgta ttcacattgc tggccacgga tgacatcagg ttgtcttccc gttgagcata
    16261 tgcattgacc ttaaggtcca tgccgttcca gctcatccgg tcgaaatgga tctccacgcc
    16321 gttacccacc agtttgactc ccggactgaa cagcgttgcc cctgcccagg ggccagcggg
    16381 aatggttgcg ataaccttgg atgacgggtt atcagaatcc actgcagtat caatatatgc
    16441 cggtatacgg gtcagagcag gtatcagttg tatccctttt tctgctacag gcgtggacag
    16501 gctggcattc tttaatgcga attgctgcgt accattctgc cctgttgttt ctgccggaga
    16561 tttattccac atagtcgttg ctatggttgg tgtactaccg gcaggatgct gatcttttat
    16621 tcttacaatt agcttttgca gccgctccat acgtttttca ctggctgctc gatcctcctg
    16681 tggtgtgccg gaagcctggc gatgagcata gttttccggt tttgctgcag gctgttgttg
    16741 cttttcctga gtgtccggaa tatcaagccc ctgtggaaga ctggcaatga acgtctgatt
    16801 attgcgggct gcagctgcgg cccctcgttc attatccgct cttaacagtt cacgatagtg
    16861 aggcgtttcg gtaacgctgc gcgttccgcc gcttgctgca ctgttaatgt tcacggctga
    16921 ctgcatatcg ctgttactcc ctgatagttt ggaaaacgcg atatagccgc cgccaaataa
    16981 gatgatgctg cccagtccaa gcagggcagc cagtttcttt ccggatttac cggcatcctg
    17041 ttcagcactc atgattactc cagttctggt gtcacgtgaa tggactggcc attcacagag
    17101 aaagtcagta gtggggtgac gggaagtttc cagagatgag ttccgtcggc agaggacaag
    17161 gtctgttcaa attcatcgcg caacatcgcc cgggaacgaa ccagaaggtc atcgccgtgt
    17221 tgccagatcg ttgtatccgg gacattacct gtgaatttca gcctcttaac tgaagggtcc
    17281 cggggaggaa taccatccag aaatgcctgt aatgttgcat catgcagggc gatcttatcc
    17341 gtcgggatgg agacaaccgg gctggttggt ccctgccgtg gtattcgcag atccagacga
    17401 ctgtccattt cctgactgga tgacggcgta tctgtttcgc cactggttac gttcaggatg
    17461 accggtaccg acagaccttt caggtagaca gaaatatttc cggatgccca tggccgcaat
    17521 ggtgtgattg tcaccatgtt ctcgttgtac tgcacatcaa agagttttgg tgccgcgtta
    17581 taaggaggat cggattgtgg ccatggtgaa ccgttgatat cggtaaatgt caccacgctc
    17641 aggttgttca tggctgtgcg aacaagagga aggctggcac cgggggacag attaactgtc
    17701 agagagctga tacggggtac aacactggtg atgggcgcat tgatggcccg ctcgttatcc
    17761 gccatgagac tgcgtagtga gcgaatctca tcggcggaga gctcagaatc aagccttgcc
    17821 gcatcctgta ccagcgggct ggcctgcccg ggggcaggca gctcaccttt taccagtgtt
    17881 gccggaacac ctccggtgtt ttgggaggcg ttctgaacgt gatcgctggc agaatttgtc
    17941 gtttgtggtg tgcgtgcgtt ctgccatcct gcgttatctg cagacgtggc ggcgctgcac
    18001 accaggagca gtgtgctcag taaataacgt gattgcatat tacttatctc aggagttcgg
    18061 tccggcgtta cgggagatca tctgccggat ttccataccg gatggtttca gtctggggtc
    18121 aacacgctgg atggtgattt cgaaaacaaa agactgttct ggcttgctgg ttgtctggcc
    18181 gaccagacgc atacggacag gatactggaa tgtccagaac catacgccgt cggacatttg
    18241 tccctgtcgt accagaacgc cagcacctgt cgtggcggtc agattcatct tctctttctt
    18301 gatcgtctcc agaatgttag atgcctgtaa tgcattcaca tatccgacaa aaccttcgtc
    18361 agaaaaacgc ggagacaggg aggagatctg tgaacgatag tgcacaaaat ccaggttgaa
    18421 tgcggtcgcc agtgcctggc tgccaaaggc cattgcatca ttctgactcc atgctggctg
    18481 acttgttggt gccagcctga ccagacgacc attgtctgta gcgaaatatt cgcgctccac
    18541 attcgttgag tgccagtact gaatggcgtt accagtaata ctgataagaa gacatgtgcc
    18601 cgtacataaa agcgctttta tcagcgcagg gacattattt gcccgctgtt ctgactgctt
    18661 catagccctg attgtgctct tcagtatttc cgggtcaggt gccggatgtg atggcgagga
    18721 tggctcggta ttttcggtca taggttgtag tccttccagg gatcaatatt ccccggcagc
    18781 ttttcattca ccttgtccac gaccgcgtcg cagacctggt tttccagctg cgacagaatg
    18841 tcgtcaaggc tggggaactg ggggaggttt atgctgattt ttttgattcg tgacagacag
    18901 gactgcaggg atgaggaaac atcgttttca cgttgtgacc atgcatccgc agctgcacgt
    18961 gctcgctcat atcctgtatt actaccggcc tgtgccgcgc tacccgcacg acaggttgca
    19021 gcacttgccg tggatgaaat caggataagg ccgagcacgg caaccagaga cttatgcatg
    19081 ttcatatggc ttccttaccg gctcatcggc cggggctgtg ggagagatcc tgatgcagag
    19141 ttttttcctg cctgtcctcc ggcttttggc tgttggacca ctgctgccat gactgctcga
    19201 agcccaggtt ccggtacaac gcatcaggtt gtttttccgg cgtgtagaca gcagcaaaaa
    19261 gcggttcttc ccgggttttc agatgaaact gcataagctc cggtgcaggg cgattcgtgc
    19321 tgtcagcatc gtggtttacc gcttcaccat aacctgcaaa gcgtaaacct ttgtcggtca
    19381 gcgcattgat aaccagctga ctgttgtcgg ctgatggggt ggccagtgcc ggatacaatg
    19441 tgccgttcag ccggaccaca ccctgcagat gttcatcggc actctggaca agtagcaggt
    19501 tgagttcacg cgtatcctta tccactgatc ccagaacagg tgtcagcttg tcaggggctg
    19561 cgctggttat ttctgttacc tgatttgcgt tctgtggcgc ggagaccgga tgtcggtctt
    19621 caccgataaa cagatgtgag ccgacgtttt ctggcatacg taacgctttc atctcttcgg
    19681 gccacccctg gctgatttgc gcagccactt tcataaaggt ttcgaccgga atcgcctgaa
    19741 tacgctgacc tttatccgga ttatcatgtg ataccgagat cccgttggta actgactcca
    19801 tggaccactt attccggaaa cgtgcttttt gctctcccgg gaggatgaat tgttcgcggt
    19861 tctgaagtgt cagtgtcacc acgtcacctt tctggtggtt tttgaccaga ctttccagtt
    19921 gttcccccca gtagagtttg ctgccactgc ggtttttaag ctcaacaaag aaactctctc
    19981 ctttgccggg tgcatattca gcacgaccaa aattgtgcag tacggcagtc acgctctctc
    20041 ccgggcgaag ttttcctggt ggttcttcac cttgtgccgc atctgccgtt ttgccaggtt
    20101 cagccggtga tgatgcaggg acggtgctga cggttgatgc ttcaggggct actggggacg
    20161 ccggggattg tgtggcttct ttttccttaa cagggtctgg gactgctggt tgttcagggg
    20221 tattccggtt aagttccggt gttggcttat gggtgacaac agtatcctta ccgacagcca
    20281 tttccttgcg taatttttcg agcgccgctc gctgatccgg gaatttcatg cgcactttga
    20341 tgttgtgctc gatgatcaac tgcatcgctt tttgcttaaa ggcatcactg cccgtcagct
    20401 caatgacacc accataaaaa tcagtggcca cagcaagtgc agccagaaca ctgcgatcgt
    20461 cattgctggc accgttgacc atttccagcc ggtttacccg gtcaatgaag gcaggtttgc
    20521 catccagttt gtacaggacg gttctgtctg ctcgttcttc accttccagc gatttaatga
    20581 tttcgtccag attgagcgat atcggtacat cagggcgttc cggggcgtag acaatactgt
    20641 caggtgccgg tgtggtcgtc gtggtttccg gtgagggggt gggatcactg ttctcatgcg
    20701 cctgtgaatc cgttttttta cgcgtaaaaa agccacgcgc tttgtcgaga aacgatgaag
    20761 tattatcaga agatgatgtg ctctgaacag gttgctgtgg agtggggtca tcccggacag
    20821 ccggttctac tgtatctgtt gtggcctgaa gttctgactc atcagcctga acgtggcctg
    20881 tgtccggttg cgacggcata tcactgtttt cctgcagatg ctcaggattt tcattgttca
    20941 tcagctcctg atatgcgctg tagttttcgt attcttcggg gccatctgac tcaggaagga
    21001 acggctctgg ctgaagggga atgtcttcag ttacatggtt gaccgcctct tcccggggag
    21061 tatccgggga tattccggtg ctttgtttgt catcaggctc cgtcatcacg gtcatttcct
    21121 ctttgactgc atcaggggaa ggtgatggct cggctgattc attcatggtt tcagatgagg
    21181 gactgtcttc aggggatgcg ggggtggcag caggggccgg tatttcaggt gaagtgccgg
    21241 attcctgttg tgactcgttt ttttccggtt gtgacagttc ggcttcaaat tcacgccagt
    21301 ctttatctgt ggccaccgag acaacttttt ccggaggttc cgccggatga gcgggttcat
    21361 caggggcaga cgccgttgta ctttccgggg acgccggtga ttctgcaggg gagtcctgaa
    21421 ctacagagct ttccgtttgc gcctgaacgt tatcagcgtg ccgctcatct ttgccgttat
    21481 tttcagtggc gtgactggta aatgactgtg tttcatcagc aatgttactg ctggaagcag
    21541 ctggttgttc ctgctgcgtg gcggacggca gagaaggtcc ctggtcatgg ctgtgccctg
    21601 tatcgggtga tgtttcagga tattctgtcg gagtcatggt tttttcctca gtgtggaccg
    21661 gtgtaaaaac gaggttgcct atcaccagtg cctcgcgcat ttccggggtg ttactgttgg
    21721 gggtggaaac cccgatgtgt tctaactggg cgttgatgac atgctgaaaa gcggcccgtc
    21781 cccggcttgc gtccaggtca ttgaaatcgg tcagcccctg cgctttttcg gcatcggtga
    21841 acgcaggaaa gatgaccgat cctcctgtca gttctgcggc ttttgttgca ctgacgatac
    21901 ctttgttaac ccggattgca tgatcgttgt cggcacagaa gataaagggg ctttgcgtcc
    21961 acttttgccg ggcattttcg gcaacggcaa tcatgttgcc tccatccacc gtcatcagta
    22021 ccggaaggcc cgtggcttcg tgcagggagg ccgcagtggc atagccctcg gcaaacagta
    22081 ccggctgacc gttgcgcggt gtgcccagcg caaaccagtt accggttttc tccgaatcta
    22141 tcaggatgcg ggcatctttc cccccggtga ccggaatacg ctggtaagag cgtatcgcgc
    22201 cgttacggtt actgaaggga ataaccagtt catttttgtt gtttacccgc acccccggtg
    22261 cagcctgaat gcctttccgg gtcaggtatt catgtgctgt ggcctgcggc catttgttga
    22321 tatagcggcg ggcatacgcg gcctgtcggt tgtactgtgc tttcagttcc cgctccgcgt
    22381 cctccctgcg ttgcattgaa tgcgctttca ggtggagtct tgcgcgggga tctgtctgtt
    22441 ctccgccgga gaaggtccag gtgatcggcg aattatccgc gctgcgataa tccctgtacc
    22501 agccggcagg tctgccgtcc aggaatccgc gatatgcgcc acttttttgt ccttttttgt
    22561 catcggcggt cgggacacgg tggattttcc cgtccatgac cggcagttct ttcagaacca
    22621 gtccggcatt ctccagaacc tgggcaaact cggtgaccgg atcactgccg ttcatggaga
    22681 cgtcctgtgg ccggggcagc cagcggtcca gcctggacag gtccgccccc gggcgggcga
    22741 accagagttt gtgctccttg tcccagatga cgggcgtgtg cccgttcatc gggggatgtg
    22801 cttttttgag atcttctatt tcatctgcag gcacggcaag ccaggtgcgg tagcgggata
    22861 cagacggcat gagtgcttct cctgaaaaaa gacggaaaaa aaccaccgcc ggaagccggg
    22921 ggtggctggg gggatagaaa atcgggggag aaatgtttgt cagggtctcc agtctgtacc
    22981 cttattccag cgatcctgcc agtgttcaag atattgtgcg gctacatccg gcatattctt
    23041 gagcacaacg acattctctg aattcttgcg tgcagcagca cgagagaagt tataggagcc
    23101 agtttcaacc gtgtttccgt caacaatgat gactttgtcg tgatggatgg ggaaggcatc
    23161 tacagtccgg agaggaatgt ccagcaggtt gatatatttc atcgcttcct gacttgcccg
    23221 gttcgtattc cccttgtcat caacaacaat acggacgtcc actccgcgtt ttttcgcttt
    23281 tgccagcgca tgcattacat ccgggtcggt gaaggaatat gccatcatcc tgatggagct
    23341 ttccgcactg ttgattgtgt caagcaccag agcggatgcg cttccttcag gagaaaagcc
    23401 gaccttcacg gatggcgtgt cgatcattgc aaacgatggt gtcgtgaaca gtaacgggca
    23461 tgccagtagc gatacccgga gcagagtaac gatagttttt ttacctgaca taaagactcc
    23521 agaatttaat cgaggccagt ccagtctcgt ggtgattccg tatggtggcg ataccaccat
    23581 ggacgaccgg aggcgatggc ccctctcaga atgctgaaaa tgcgagtaca gatcgtcctg
    23641 acagtccagc ctgaacggtc gagaataacg aaaaataaaa tgatgccggt gcaaatccag
    23701 aaggttgttt tggagggaaa ggggcatata aacagataaa tcatggtgaa cggcagaggt
    23761 acgccctgga tgttgactgg cggtgttgca tatcgccaga aaccttctct tttcatgacc
    23821 gtaactcccc tggcgggata aaaagaatcg cttctcttcg gtcaacttca ttccggatat
    23881 acatttccag aatctggtcc cggatacggc gcttctcctg ccggattatg gcatcaatgt
    23941 gatgccccca ctctgcatgc ggcattccgg aaagattgtc gcgaagctca tcgttaatga
    24001 cgatatattc ccggacggct ttacgaccac cgtcatttgt tctgaccaga acctgaacca
    24061 gaatgaactg caacagtgac aggacagccc aggccatcga atccctgatg acagggggaa
    24121 acagcccaag aagacgtgcc agcgtttcac caggagattt cgtatgcatg gtgctcaggc
    24181 agtaatgccc ggtgttgcct gcctgtaccg ctgcgtcggc ggtttcgtta tccctgatct
    24241 caccaacacc gataatttcg ggattacgtc gtacagcaga acgcagtccg gcagcaaaac
    24301 tgaccacatc acgcccgatt tctgcctgat gcggtgggag cagatcgttt tcatttccca
    24361 ggatatattc caccgggtct tcatatgtca cgattttggc gtcggggaaa ttgtccataa
    24421 tgtaacggta cagtgctgaa cacagcgttg atttccctga cccggtttca ccacatacaa
    24481 atcccagacc gcttttgcag accattgctt caagcagatc tggttcaatg ttcatggaga
    24541 gaatgtccgg tatttctgtc gggatgacac gcatggttat tgagattgct ttctcctccg
    24601 caccagatgt gccctgaatg aggtgagagc gtaaccggat gcgttctccg cgtttaagac
    24661 catagcgccc ggatgcatcc ccgttaatct gtatcgtcct gtcaaccgga ttgcctgcca
    24721 gaacgcgggg aatgacttcc cgaccgaaca gttcatcaat cagggaggac atgagtgtgg
    24781 ttggtaacgt tgcactggaa caacgcaccc gtcggccaaa gcgactgaca gatacaggtg
    24841 acccaccggt cagatccaca tcactgactt tatgtgcggc acaccagaca aagaaatgcc
    24901 gtagttcatc tgcagtgaaa cgctgaaacg gaaaaagacc aaactcatca ggaatattca
    24961 tcgggagcct ccttcagtgc ctataagcgg acgccagtga gatttctgaa gattgaattc
    25021 cgcatgattt ttaaggcgac gaacacgatc tccagtgacc agttttttcc cgtcacctgt
    25081 aacggtttgc atttgatcag aaacatccgg acgggtgatc atgccctgac gccagagaag
    25141 tgagtacagc atcatgcccc ggtaatcacg ggtgagggtt ttctggttgg cttcaagcgt
    25201 caggtcggcg ttttgtcttc cttcctgcca tccctgccgt aatgcggttt cccacacttt
    25261 tcgttgcacg ctgtcttctg gaacgacgct gccttctgta cccggagtgg ctgttgttga
    25321 aagcccccgg agaagccagt cacgccagcc cggtggattg ctgacaaagc gttcagggcg
    25381 aatgatggtc cagacccggg aagaggtccg gatttgatct ggcgtgatat gagctacgtc
    25441 ctgtgcttca tcaataaccg gtggtagcca gccttccggg ctaatcagtg gccggaaatc
    25501 gtataaggca ttaagtgtgc tttcccgggc gttgagtgcc tgaatcagct cccatgaacg
    25561 ctgagccttg ccaccacgaa atccaagcgt tttacctgcg tcggtcagca ttttccagcg
    25621 tgtttctgac agactatccg gcttttcact tcgtggttta agccatgcat taatgtcagg
    25681 tggtggcgta ttgacaccac ctggcgagat ggtaggggaa gaagaacaac cagtaagcag
    25741 tattactggc aaaatgagag aggtgagtgc tttcattttt tccggttctc cggctgcatg
    25801 aagctgacct gtaacaagcc cggatattga tggattgttg ctctccaggc tgtctgggct
    25861 tcaataagtc tcaacgtatt ggaaaacgtc atattgtagt ggcacactaa atttggccac
    25921 ctgatgaaag gtgatattct caccacaaca caaaacaggt gacttaatga acaagaaaac
    25981 caaacgaacc ttcacccctg agttcaggct ggaatgtgca cagctgattg ttgataaggg
    26041 ctactcatat cgacaggcca gtgaagcgat gaatgtcggt tcaaccacgc ttgagagctg
    26101 ggtacgccag ctcaggcgag agcgccaggg tattacgccc tctgccacac ccattactcc
    26161 agaccagcaa cgtatccgcg agctggaaaa gcaagttcgc cgtctggagg aacaaaatac
    26221 gatattaaaa aaggctaccg cgctcttaat gtccgactcg ctgaacggtt cacgatagcc
    26281 gccagactaa gtgacagcca cacggttgtc agcctgtgtt ctgctctgga aatacaccgc
    26341 agcagttacc ggtactggcg aaaacgacgc gatacggtta atccggcacg agtcaggttg
    26401 tgcagcgaaa tacgccgggc gtggaaccaa agtcggggct ctgcgggcgc gcgcactctg
    26461 gctgaaatgc tgactcaaaa cggcgtcccg atgagccgtt accgtgccgg acgtctgatg
    26521 aaatatctga acctgagcag ttgtcagccc ggaaaacatc agtataaaaa tgcccgtcag
    26581 gagcatacca gcctgccgaa tctgcttgag cgtcagttcg cagtaccgga gccagaccgg
    26641 gtatggtgtg gagatattac gtatctctgg gcaggaaatc gctggtgcta tctggcggtt
    26701 gttatggatc tttttgcccg cagggttatc ggctggagtc tgtcagcgca tgccgatacc
    26761 gcactgataa gcagtgccct gcggatggcc tatgagacgc gtggccaacc ccgggatgtc
    26821 gtgttccata gcgaccaggg aagccagtat acaggcctta aatatcaaca acttctctgg
    26881 cgttgcagaa taaatcaaag cgtcagtcgg cggggaaact gctgggataa cagccccatg
    26941 gaacgcttct tccgcagtct gaaaacagaa tgggtgccaa cgaatggtta cgcaggcaag
    27001 gacgaggccc ggcagcaaat taatgattac atattgaact actacaacag cgtcagacct
    27061 caccattata acggtgggct gacgccggaa gagtcagaga acagatacca tttttactgt
    27121 aaaaccgtgg ccaatattac ttgaccacta catatcccgg acgtgaaggt tgactggtaa
    27181 cggcaggcgg acaccattgt agttaaactg catccccctg actctggcca gttcgttcag
    27241 taattcaatt gcatcacctt cccagtcaaa ggaaaggcga tcgctgttag ctgtcagaag
    27301 atgagacccc gttgaaaccg gaagttggcc ggaaaacaca cctttgtccc ataatgtgta
    27361 ctgagtgtgc cgggtaaccg ccacatcaga ggcagcaccg tagttacgcg gaacaggagt
    27421 atgctgagaa ggggagttgc agccagccag aaaaagggcg gcaagacacc ccgcaggtat
    27481 aatacggttc ataataattc ctgagcagtt aaatcagaat gggatatacc ggtgctccgg
    27541 tacagacagg atgatgtttg tttccggcta tacttgcgtt accggaaata aatgccagtg
    27601 tggccaggta aatagcctca tagtttactg aaggtgatta ttcctgatac tggctggttg
    27661 atgtgtcgtc agacataaaa cgaatatgag cgtaatcttt aacgctccgc gccgaatcga
    27721 tccatgatgg atgactcgac gatttatgaa tcgaacgtac cggcgcttgt ccctgaaaca
    27781 gttcccggcc ctcgtccgga taacctgcaa ttcagacagg cacatgtgcc cgtacactct
    27841 tgtatcttac tgagtatatc agaatttatg taaattcaaa tatcctctat gcctctttgt
    27901 tgtattttcc gtcaagtatg agccgggtac catcgtaatg catgatcagg gcatcaaggt
    27961 acgccggttt gaatttcttg ccgagcctat ctctggccag aaatgataaa caatgattat
    28021 gtgtcattat tactatgttc cttttatgag attctcttgc gattgattca agcgtcttat
    28081 aaagatcatt attgcagtct gataaactgt ccataacgac gggttcttta ccggagaaga
    28141 attttgcagt ttgtatggtt cttaccgcat tgctggagta aatatcatat tcactgaaga
    28201 ttgttgcgaa cttaatacct tcctgctgtg ctttctctgt accggtaata gtgattccgc
    28261 ttttatccga atagcatggc atatctgaac ggtcgcaacg ttctccatgg cggaaaagga
    28321 aaataacggt gttatcagca ttaatttctt ttgcctgttc cagggtgata tcgggaagcg
    28381 agctttttgc ttttgtcact gccgctacca ggaaaacaga aaataaaaga atgaatgctg
    28441 tttttgaata agaagaacgt ggtttcatca tgttatttaa actgaatata aaaatacccc
    28501 cgggtttagt gccgggggcg gggacagtaa tcagaaagtg aactgaagtt gtgccattgc
    28561 accgtaatcg tggtcagttc ctaccaggcc ggaaacatcg atatgtacga cgtcaaatgg
    28621 agagatacca acgccagcgg tgaaggcgct tccggagttt gatgccatgt tctgacgata
    28681 accggcacgc aattgcgccc atttccatgc gttaaattca gcaccaacac ttgcaaactg
    28741 acgtttgctg tcagaagtga agccactggc aggagtcaga tcaacgtcaa gagcagtggt
    28801 aaacaggtca ttatgccagg atacaccggc ggttgcctgt ggacgaactt tgaaggtttc
    28861 tttaaagcca ttgacgactt tagtatcaat actgcgtgga atgatgttct gggcaacaag
    28921 accgacagtc cagttgtcat tcaggtctgt ataagcacca atatcggcgt tgaatccgtt
    28981 tttcgtgttg tggtacttgt caccgtcaaa atcgtctttg tcgtaatcac gaaccgttac
    29041 gttgtagtta aacaggtcca cacgctgata ttttggtgtg acgccaagag accatttttg
    29101 tccggccgtt tccagttctt tggcaaatga aattccgacg tcagtaatta cagctgcacg
    29161 cccgaatgcg cgtgaggtca gggcattctt atcgacgtcg gtaattgttc cattggcaac
    29221 tttgtccaga taatccaggt ctgcatcgtt tacttttccg tttacgctga cagttccgta
    29281 agacttgacc ataagggcaa acggcagagt gtcatttgcc atggctgcta cagctgatac
    29341 acctacctga gcatctgcat tgatattgcg gaattcctgc agacgatgtt taagatttgc
    29401 tgctgcctgt tgtacgccat gctggttatc aacagcacta tcaaacaaat cccagtcatc
    29461 tttaacatca tcagctttat ttgatacatc atcaggatca gcaacttgtg ccccaacaga
    29521 cggcagaaga aggctgaaat catcattgct gttgtgcttt gtcagtaaag ccgggtttgc
    29581 cagaggtgcc acaccataat gggaagatgc taccccggtg ccccccattg catcgttacg
    29641 tgcttcaaaa tatgacgtcg ctgctgcagc gttaaaggaa aataatacag cagatgcaat
    29701 gatggtgcgg gtaatatgat ttttcttcat catttaattt cctgtttatc agaatttaga
    29761 caaaacaacg ctatgacacg gatgtgccgt aattttttac tttggtacta tctggtattt
    29821 accagggtaa taaagacgta ttaaataagc taacaggtag tatttgccct gtaataaggt
    29881 tcccggcaag gccaggtctc aacgtgacgc gtcgcaaaga cattgttgta ttctgcctgg
    29941 ttgtatattc agttaaccat tttctttttt catcgttcag aattgttggt gataatgata
    30001 gtgccggata tattacagaa ccatttattt cttttttctg tctcaatatt accccgtaat
    30061 attctctttt cgtgtttcct gcgaagggga aactatgcca gaaaataact tcagtgttgc
    30121 tgctatcatt aataagcatt ctgaaattat cagcagtatc tggccattca ccggacaacg
    30181 ctatcggata gttttcatgg ccattcagcc agtccggatt gagattaaaa cattcagcaa
    30241 gatgttcgag agcgtcagct gaaagatagt ctgcaagtct ggccgggtcc tgtataaccg
    30301 tggagcggat attccatggc gacagcaatg ttgccagtaa tggcgcattt atatcatgcg
    30361 acagcatgat gtattcaatt ctgcttatta tgttccctgc ggtgttatcc gcaggcataa
    30421 acatattatg aagagcatcc tcaagcagga ttcgggttaa atcagcggtg gatatggcta
    30481 attgttcgct cattatcccc agaacttctc ttaccccttg ggggtaacgg acctgcagag
    30541 gtgtcgggtt tatctgacga ccgttgatga gtttttcgcc gattgctttt ttgtccagcg
    30601 agagcatcat tcgggccgca ataccggtcg tattcagtct cattgttcct ccgactgaaa
    30661 aagtcttttc agctcatgtg gttattgtat cttcggtgtt ttttctggtg aaacgaataa
    30721 tttcgatcat gcagatcgaa tgtgtttggc gagttgttgc aactcgcctt tctgtatgtg
    30781 ttttgaatga tgtttacagc ggggtgctgc ccgggttcag agggcaaatc ataatgtagt
    30841 cagggtcgtt aacaggtaac gctccgggag atgggacacg ttcgcctttc tgtgctgcaa
    30901 tggccagtgt acgcaacaaa acctcactgg cgcggtgaac tgcttcctct ttcgttgtgg
    30961 cagtgacgtg aaggttatca aaatcaccga tctcaatacg atgcgctttc tgcccatttt
    31021 cttcattgat ggtcgtgata tgggcaggat acggcacaaa ccatgctgcc actttttgtg
    31081 tctgccgccg gcgggcatca agtttactga ccagttgcca tgcacgaaga tgagtatacc
    31141 gtttcagcat attcatggaa cgatgtcctg atattgcagc aatctccatt acattcaggc
    31201 tacccagttc gaagaagcgg cttattgctt catgccgtag atcgtgaaaa tgcaggtcct
    31261 cgatgcgaag tcgttgtgtg gctattctcc aggcattttt aaagccggat gcggtgtaat
    31321 caaaaacatt gccgtggaga ttaacgggca tcatctgaag aaagttacgg gcacgtctgg
    31381 acagaggaac atcccgtgag tgaccgtttt tggtttcagg taaatgagcc acaccgtggc
    31441 gcaaatcaat gtgctcccaa cgtaaggcca gtatttcgcc ctgccgcatg gctgtctcaa
    31501 gggcaagatg gaaaatgaca tacaacatca gatttttttc gcggaaatag cgagaaaggc
    31561 gacgttcttc tgaagacgtt agccggcgat ctcgtccgga ggatactttc ggcttgcgaa
    31621 ccagttcaac cgggttagta cgacaggttc cccattcaac acgagcaata ttgaacagag
    31681 atgacagaag ggcgagttca agacgtacag tattacctgt aatgggttta cccgttcggg
    31741 ggttgatttc tgctaaacga acgtctctgt atgtagcaat atcaacggtt gttatttcgt
    31801 ccatattccg aagggcaatg ggatatcgct tgataacatt gctccggtaa aactcctgtt
    31861 gatgcccttt cttgtgaaca gaaactgttt tcaggtactt atccagtgcg cgtgacaggg
    31921 acattttacg gatgcgtgga gacggcatgc taacctcaaa agaggcaaaa aataatgcca
    31981 aca
//
LOCUS       DAJTVJ010000039        31796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000039 DAJTVJ010000000
VERSION     DAJTVJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.039"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="OO448_001746"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="OO448_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6336977.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="OO448_001747"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="OO448_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6336978.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="OO448_001748"
     CDS             1894..2751
                     /locus_tag="OO448_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6336979.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OO448_001749"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OO448_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OO448_001750"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OO448_001750"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6336980.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OO448_001751"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OO448_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="OO448_001752"
     CDS             complement(7411..8658)
                     /locus_tag="OO448_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6336981.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="OO448_001753"
     CDS             complement(8680..9006)
                     /locus_tag="OO448_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6336982.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OO448_001754"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OO448_001754"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6336983.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="OO448_001755"
     CDS             10395..11651
                     /locus_tag="OO448_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6336984.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /locus_tag="OO448_001756"
     CDS             11766..12092
                     /locus_tag="OO448_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6336985.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12233..13471)
                     /locus_tag="OO448_001757"
     CDS             complement(12233..13471)
                     /locus_tag="OO448_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6336986.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13807..15009
                     /gene="nupC"
                     /locus_tag="OO448_001758"
     CDS             13807..15009
                     /gene="nupC"
                     /locus_tag="OO448_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6336987.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15059..17247)
                     /locus_tag="OO448_001759"
                     /pseudo
     CDS             complement(15059..17247)
                     /locus_tag="OO448_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17456..17531)
                     /locus_tag="OO448_001760"
     tRNA            complement(17456..17531)
                     /locus_tag="OO448_001760"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Ala,seq:ggc)
     gene            complement(17571..17646)
                     /locus_tag="OO448_001761"
     tRNA            complement(17571..17646)
                     /locus_tag="OO448_001761"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17611..17613),aa:Ala,seq:ggc)
     gene            17867..18226
                     /locus_tag="OO448_001762"
     CDS             17867..18226
                     /locus_tag="OO448_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6336988.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18228..18620
                     /locus_tag="OO448_001763"
     CDS             18228..18620
                     /locus_tag="OO448_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6336989.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OO448_001764"
     CDS             complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OO448_001764"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6336990.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20346..20421
                     /locus_tag="OO448_001765"
     tRNA            20346..20421
                     /locus_tag="OO448_001765"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20379..20381,aa:Val,seq:tac)
     gene            20467..20542
                     /locus_tag="OO448_001766"
     tRNA            20467..20542
                     /locus_tag="OO448_001766"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20500..20502,aa:Val,seq:tac)
     gene            20589..20664
                     /locus_tag="OO448_001767"
     tRNA            20589..20664
                     /locus_tag="OO448_001767"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20622..20624,aa:Val,seq:tac)
     gene            20669..20744
                     /locus_tag="OO448_001768"
     tRNA            20669..20744
                     /locus_tag="OO448_001768"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20702..20704,aa:Lys,seq:ttt)
     gene            20775..20900
                     /locus_tag="OO448_001769"
     ncRNA           20775..20900
                     /ncRNA_class="other"
                     /locus_tag="OO448_001769"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21020..21361
                     /locus_tag="OO448_001770"
     CDS             21020..21361
                     /locus_tag="OO448_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6336991.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21352..22278)
                     /locus_tag="OO448_001771"
     CDS             complement(21352..22278)
                     /locus_tag="OO448_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6336992.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22368..23366
                     /locus_tag="OO448_001772"
     CDS             22368..23366
                     /locus_tag="OO448_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6336993.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23363..23581)
                     /locus_tag="OO448_001773"
     CDS             complement(23363..23581)
                     /locus_tag="OO448_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6336994.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23583..25598)
                     /gene="ligA"
                     /locus_tag="OO448_001774"
     CDS             complement(23583..25598)
                     /gene="ligA"
                     /locus_tag="OO448_001774"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6336995.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25669..26655)
                     /gene="zipA"
                     /locus_tag="OO448_001775"
     CDS             complement(25669..26655)
                     /gene="zipA"
                     /locus_tag="OO448_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6336996.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26885..27646
                     /gene="cysZ"
                     /locus_tag="OO448_001776"
     CDS             26885..27646
                     /gene="cysZ"
                     /locus_tag="OO448_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6336997.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27831..28802
                     /gene="cysK"
                     /locus_tag="OO448_001777"
     CDS             27831..28802
                     /gene="cysK"
                     /locus_tag="OO448_001777"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6336998.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29186..29443
                     /gene="ptsH"
                     /locus_tag="OO448_001778"
     CDS             29186..29443
                     /gene="ptsH"
                     /locus_tag="OO448_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6336999.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29488..31215
                     /gene="ptsI"
                     /locus_tag="OO448_001779"
     CDS             29488..31215
                     /gene="ptsI"
                     /locus_tag="OO448_001779"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6337000.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31256..31765
                     /gene="crr"
                     /locus_tag="OO448_001780"
     CDS             31256..31765
                     /gene="crr"
                     /locus_tag="OO448_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6337001.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7844 a         8384 c         7906 g         7662 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caagattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12181 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12241 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12301 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12361 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12421 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12481 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12541 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    12601 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    12661 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    12721 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    12781 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    12841 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    12901 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    12961 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    13021 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13081 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13141 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13201 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    13261 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13321 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13381 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    13441 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13501 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13561 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    13621 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    13681 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    13741 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    13801 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    13861 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    13921 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    13981 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    14041 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14101 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14161 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14221 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    14281 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14341 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14401 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14461 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14521 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    14581 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    14641 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    14701 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    14761 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    14821 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    14881 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    14941 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15001 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15061 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15121 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15181 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15241 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15301 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15361 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15421 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15481 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15541 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    15601 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    15661 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    15721 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    15781 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    15841 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    15901 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    15961 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16021 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    16081 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    16141 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    16201 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    16261 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    16321 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    16381 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    16441 aacagccctg gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    16501 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    16561 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    16621 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    16681 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    16741 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    16801 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    16861 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    16921 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    16981 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    17041 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    17101 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    17161 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    17221 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    17281 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    17341 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    17401 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    17461 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    17521 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    17581 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    17641 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    17701 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    17761 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    17821 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    17881 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    17941 ggtacgcaag gagggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    18001 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    18061 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    18121 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    18181 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    18241 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    18301 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    18361 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    18421 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    18481 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    18541 agggaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    18601 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    18661 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    18721 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    18781 cccgccccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    18841 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    18901 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    18961 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    19021 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    19081 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    19141 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    19201 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    19261 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    19321 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    19381 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    19441 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    19501 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    19561 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    19621 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    19681 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    19741 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    19801 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    19861 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    19921 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    19981 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    20041 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    20101 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    20161 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    20221 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    20281 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc ggctccacac
    20341 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20401 cgatcccgtc atcacccacc aacatacttt atgtagtctc cgccgtgtag caagaaattg
    20461 agaagtgggt gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt
    20521 tcgatcccgt catcacccac caatttctcg ccagctaaat ttcttgtaaa aatgtgaagt
    20581 accgaagtgg gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg
    20641 gttcgatccc gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac
    20701 ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc
    20761 taaaggcgct tttttgctat ctgcgatact caaagattcg aacctgcagc aggtttgagt
    20821 tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag
    20881 tcatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc
    20941 gatttgtaag attgattttt cttctcaagt tccttgttct tctgccgata ttacctcgta
    21001 cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac
    21061 acagagtagt aacagggcat cacaaggtag cgatgttgct tcaaaaatcg cgcggataag
    21121 tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc
    21181 tgaagacaaa caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca
    21241 actggcgcag ttgcagagac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc
    21301 tctgaatgta tcccttctaa atcctgttga aaatacgact catatcgata tctatatctg
    21361 atacaacgga gccccttccc gaccagccat tagcgcattg ccctggtctc gtaccacgtc
    21421 ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg
    21481 gcgttcaaca accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag
    21541 cgccagtgcg ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc
    21601 cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc
    21661 aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc
    21721 ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa
    21781 aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat
    21841 gcaaaaatcg acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg
    21901 caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag
    21961 atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat
    22021 acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc
    22081 caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac
    22141 accagtatga ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat
    22201 acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg
    22261 ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca attttatttc
    22321 tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc
    22381 gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc
    22441 cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc
    22501 tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact
    22561 ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac
    22621 tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact
    22681 tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta
    22741 acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac
    22801 cattgctagt tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg
    22861 gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga
    22921 ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc
    22981 tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg
    23041 gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg
    23101 tagttaacgt ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg
    23161 tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc
    23221 ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga
    23281 tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc
    23341 aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca
    23401 gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga
    23461 actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc
    23521 ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca
    23581 tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc
    23641 tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc
    23701 gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc
    23761 gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc
    23821 tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg
    23881 atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg
    23941 ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcac ttccagcgtg
    24001 ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca
    24061 tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt
    24121 gccgattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga
    24181 tctgccggag tgtggacata ttctttttca accagctgat cgatgatttt gtcgcccatt
    24241 ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca
    24301 ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca
    24361 gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca
    24421 gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta
    24481 tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc
    24541 accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta
    24601 cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta
    24661 aacgctaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt
    24721 gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc
    24781 actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg
    24841 accggcaacc cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg
    24901 ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata
    24961 cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc
    25021 ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact
    25081 tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg
    25141 cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg
    25201 gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc
    25261 tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa
    25321 gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt
    25381 atctggctga aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta
    25441 atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca
    25501 gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc
    25561 gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa
    25621 cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc
    25681 gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg
    25741 gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag
    25801 catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac
    25861 acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc
    25921 caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat
    25981 atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc
    26041 gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc
    26101 cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg
    26161 ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac
    26221 ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg
    26281 catcggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg
    26341 gatcggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg
    26401 acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac
    26461 ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga
    26521 cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca
    26581 gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg
    26641 caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt
    26701 actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca
    26761 caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg
    26821 cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct
    26881 gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg
    26941 ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa
    27001 tattttgttg atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc
    27061 gactctcatg agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct
    27121 ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat
    27181 tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac
    27241 accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga
    27301 atggcaaaaa tttgcctggt atctgccgcg cgcaattgta ttactaattc tttacttcat
    27361 ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt
    27421 agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg
    27481 caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt
    27541 tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc
    27601 gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta
    27661 ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca
    27721 tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga
    27781 gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga
    27841 tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg
    27901 gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc
    27961 gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg
    28021 aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc
    28081 gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc
    28141 tgaaagcgtt aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa
    28201 tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat
    28261 tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag
    28321 ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg
    28381 gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg
    28441 agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt aaacctggcc
    28501 cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg
    28561 tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg
    28621 aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgcg gcgttgaaac
    28681 tacaagaaga tgaaagcttt accaataaga atattgtggt tattctacca tcatcgggtg
    28741 agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt
    28801 aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg
    28861 cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga
    28921 ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt
    28981 atgatttggt tcaattcttc ctttaacggc ataatgttta atgacgtacg aaacgtcagc
    29041 ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa
    29101 ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac
    29161 taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa
    29221 cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga
    29281 aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac
    29341 tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa
    29401 agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta
    29461 aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc
    29521 gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt
    29581 tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca
    29641 gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc
    29701 atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc
    29761 ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag
    29821 gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt
    29881 gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc
    29941 attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg
    30001 aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct
    30061 atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag
    30121 gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca
    30181 accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca
    30241 gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta
    30301 tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc
    30361 gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa
    30421 gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt
    30481 cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag
    30541 aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc
    30601 gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg
    30661 atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag
    30721 gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa
    30781 acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc
    30841 ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca
    30901 cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct
    30961 tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct
    31021 acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg
    31081 cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag
    31141 gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa
    31201 aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg
    31261 tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga
    31321 gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt
    31381 tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc
    31441 gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc
    31501 tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga
    31561 aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga
    31621 atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc
    31681 caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac
    31741 cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc ccatcg
//
LOCUS       DAJTVJ010000040        30870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000040 DAJTVJ010000000
VERSION     DAJTVJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.040"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..948
                     /gene="tuf"
                     /locus_tag="OO448_001781"
     CDS             <1..948
                     /gene="tuf"
                     /locus_tag="OO448_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6337002.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGD
                     NIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1178..1561
                     /gene="secE"
                     /locus_tag="OO448_001782"
     CDS             1178..1561
                     /gene="secE"
                     /locus_tag="OO448_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6337003.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1563..2108
                     /gene="nusG"
                     /locus_tag="OO448_001783"
     CDS             1563..2108
                     /gene="nusG"
                     /locus_tag="OO448_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6337004.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2267..2695
                     /gene="rplK"
                     /locus_tag="OO448_001784"
     CDS             2267..2695
                     /gene="rplK"
                     /locus_tag="OO448_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6337005.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2699..3403
                     /gene="rplA"
                     /locus_tag="OO448_001785"
     CDS             2699..3403
                     /gene="rplA"
                     /locus_tag="OO448_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6337006.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3816..4313
                     /gene="rplJ"
                     /locus_tag="OO448_001786"
     CDS             3816..4313
                     /gene="rplJ"
                     /locus_tag="OO448_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6337007.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4380..4745
                     /gene="rplL"
                     /locus_tag="OO448_001787"
     CDS             4380..4745
                     /gene="rplL"
                     /locus_tag="OO448_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6337008.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            5065..9093
                     /gene="rpoB"
                     /locus_tag="OO448_001788"
     CDS             5065..9093
                     /gene="rpoB"
                     /locus_tag="OO448_001788"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6337009.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9170..13393
                     /gene="rpoC"
                     /locus_tag="OO448_001789"
     CDS             9170..13393
                     /gene="rpoC"
                     /locus_tag="OO448_001789"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6337010.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="OO448_001790"
     CDS             complement(13635..14768)
                     /gene="thiH"
                     /locus_tag="OO448_001790"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6337011.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="OO448_001791"
     CDS             complement(14765..15535)
                     /gene="thiG"
                     /locus_tag="OO448_001791"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6337012.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="OO448_001792"
     CDS             complement(15537..15737)
                     /gene="thiS"
                     /locus_tag="OO448_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6337013.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="OO448_001793"
     CDS             complement(15721..16476)
                     /gene="thiF"
                     /locus_tag="OO448_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6337014.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="OO448_001794"
     CDS             complement(16469..17104)
                     /gene="thiE"
                     /locus_tag="OO448_001794"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6337015.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="OO448_001795"
     CDS             complement(17104..18999)
                     /gene="thiC"
                     /locus_tag="OO448_001795"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6337016.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19080..19180)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="OO448_001796"
     CDS             complement(19233..19709)
                     /gene="rsd"
                     /locus_tag="OO448_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6337017.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19804..20577
                     /gene="nudC"
                     /locus_tag="OO448_001797"
     CDS             19804..20577
                     /gene="nudC"
                     /locus_tag="OO448_001797"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6337018.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMTEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20617..21681
                     /gene="hemE"
                     /locus_tag="OO448_001798"
     CDS             20617..21681
                     /gene="hemE"
                     /locus_tag="OO448_001798"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6337019.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21691..22362
                     /gene="nfi"
                     /locus_tag="OO448_001799"
     CDS             21691..22362
                     /gene="nfi"
                     /locus_tag="OO448_001799"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6337020.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22405..22995
                     /locus_tag="OO448_001800"
     CDS             22405..22995
                     /locus_tag="OO448_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6337021.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23182..23454
                     /gene="hupA"
                     /locus_tag="OO448_001801"
     CDS             23182..23454
                     /gene="hupA"
                     /locus_tag="OO448_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6337022.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23467..24162
                     /locus_tag="OO448_001802"
     CDS             23467..24162
                     /locus_tag="OO448_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6337023.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="OO448_001803"
     CDS             complement(24164..24589)
                     /gene="zraP"
                     /locus_tag="OO448_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6337024.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24827..26203
                     /gene="zraS"
                     /locus_tag="OO448_001804"
     CDS             24827..26203
                     /gene="zraS"
                     /locus_tag="OO448_001804"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6337025.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            26200..27525
                     /gene="zraR"
                     /locus_tag="OO448_001805"
     CDS             26200..27525
                     /gene="zraR"
                     /locus_tag="OO448_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6337026.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27522..28811)
                     /gene="purD"
                     /locus_tag="OO448_001806"
     CDS             complement(27522..28811)
                     /gene="purD"
                     /locus_tag="OO448_001806"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6337027.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28823..30412)
                     /gene="purH"
                     /locus_tag="OO448_001807"
     CDS             complement(28823..30412)
                     /gene="purH"
                     /locus_tag="OO448_001807"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6337028.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7227 a         8035 c         8517 g         7091 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      721 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      781 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      841 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      901 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      961 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
     1021 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
     1081 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
     1141 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
     1201 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggt
     1261 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
     1321 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1381 cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1441 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1501 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1561 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1621 gccgcgtagc aacgtcgcta cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1681 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1741 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1801 ggcacctggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1861 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1921 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1981 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     2041 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     2101 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     2161 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     2221 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     2281 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     2341 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2401 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2461 ttctttcact ttcgttacca agactccgcc ggcagcagtt ctgctgaaaa aagcggctgg
     2521 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2581 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2641 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2701 ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta
     2761 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2821 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2881 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2941 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     3001 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     3061 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa
     3121 agtgggtact gtaacgccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     3181 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     3241 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     3301 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3361 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3421 tgattttgtc tacaatctta cccccacata taatgcttaa tgcagacgta tatccgagat
     3481 attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga
     3541 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3601 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3661 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3721 tgtttctgct caccgtaatt aagacgctct ctccgcttgg aggagtgaag tgagttccag
     3781 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3841 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3901 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3961 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     4021 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     4081 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     4141 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     4201 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     4261 gtaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     4321 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4381 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4441 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4501 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4561 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4621 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4681 aagacgacgc agaagcactg aaaaaatccc tggaagaagc tggcgctgaa gttgaagtta
     4741 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgactttttg
     4801 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4861 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4921 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4981 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     5041 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     5101 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     5161 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     5221 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     5281 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     5341 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5401 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5461 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5521 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5581 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5641 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5701 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5761 cgtgataaca agctgcagat ggagctggtg ccggaacgcc tgcgtggtga aactgcatct
     5821 ttcgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5881 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5941 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     6001 gcgaacatgg agctgagcct ggatctgttg gctaagctga gccagtctgg tcacaagcgt
     6061 attgaaacgc tgttcaccaa cgacctggat cacggcccgt atatctctga aaccttacgt
     6121 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     6181 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     6241 gaccgttatg acctgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     6301 gaaatcgaag gttccggtat cctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6361 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6421 atccgttctg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6481 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggacatg
     6541 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6601 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6661 ctcggcccgg gtggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6721 actcattacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6781 aactctttgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6841 aaggtgaccg acggtgttgt aactgacgaa attcactacc tttctgctat cgaagaaggc
     6901 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6961 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgtgacca ggttgactac
     7021 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     7081 cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact
     7141 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     7201 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     7261 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     7321 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7381 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7441 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7501 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7561 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccggaaga gatcaccgct
     7621 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7681 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7741 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7801 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7861 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7921 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtttgttc
     7981 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     8041 ccgcgcgatc gctggctgga gctgggcctg accgacgaag agaaacaaaa tcagctggaa
     8101 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     8161 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggtg
     8221 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     8281 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     8341 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8401 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8461 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8521 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8581 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8641 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8701 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8761 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8821 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8881 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcacc
     8941 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     9001 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     9061 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     9121 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     9181 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     9241 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     9301 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9361 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9421 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9481 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9541 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9601 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9661 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9721 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9781 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9841 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9901 actgccgcca gatctgcgtc cactggttcc gctggatggt ggtcgtttcg cgacttctga
     9961 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
    10021 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
    10081 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
    10141 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
    10201 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
    10261 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
    10321 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10381 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10441 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10501 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10561 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10621 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10681 ggacgttgtt ctgggtctgt actatatgac ccgtgactgt gttaacgcca aaggcgaagg
    10741 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10801 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgcaaacg gtgaattagt
    10861 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10921 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10981 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    11041 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggta tcgatgacat
    11101 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    11161 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    11221 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    11281 aaccgtgatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    11341 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11401 tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11461 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11521 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11581 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11641 ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11701 tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct
    11761 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11821 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11881 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11941 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    12001 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    12061 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    12121 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    12181 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    12241 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    12301 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12361 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12421 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12481 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat
    12541 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12601 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12661 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12721 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12781 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12841 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12901 gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12961 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    13021 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    13081 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    13141 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    13201 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    13261 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    13321 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13381 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13441 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13501 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13561 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13621 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13681 cataccggct gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg
    13741 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13801 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13861 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13921 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13981 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    14041 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    14101 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    14161 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    14221 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    14281 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    14341 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14401 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14461 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14521 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14581 cgctgcgccc gttgggccag tggttccaga tagccactgg cggctggcga taacagcgcc
    14641 atcatatcgt cgcgggtgag ttgcgaggca tttagcgccc gttctacgtc agcagccgtt
    14701 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag
    14761 gttttcatgc cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac
    14821 tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg
    14881 ccttcgccat gttgacggga tcgtccgcta cggcaatcgc cgtattcact aacaccgcgt
    14941 cggcccccat ttccagggcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa
    15001 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    15061 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    15121 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    15181 gcatttcggc ggctttcaga gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    15241 gaatctctaa ttttaaccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    15301 tggcttcttc cgctgttttc gccccggaag tatttggcag cagggtcaca cccgccgcga
    15361 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15421 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15481 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15541 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15601 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15661 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15721 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15781 caggctgcgc cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc
    15841 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac
    15901 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag
    15961 gcagcggtaa cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc
    16021 gaccgcgctg gcggtgataa gcggcgtgtt gagtaccacg caggcggcat taatctcctg
    16081 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt
    16141 taacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatatcgg gattcaactg
    16201 tgtcagtcgc tgatggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag
    16261 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    16321 gacgccagcc cccgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16381 cagcacctgg ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcagaat
    16441 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16501 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16561 tgccgacacc cgttgctatc accgcaggcg cgcgcgccag actgataccg ccaatcgcca
    16621 cggtgggata atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg
    16681 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16741 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16801 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16861 catacgcctg atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16921 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca ttgcgccgat
    16981 ctttgatgcg tagctggagg gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    17041 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    17101 acatcacgct tcctccttac gcaggtagat ttctccgcct ctggcgcgga agttctccga
    17161 catatccgcc attcccactt caatggtttg cgcggcggcg taatcacgta cttcctggct
    17221 gattttcatc gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga
    17281 ctcttgcggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    17341 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17401 cgcgcctgga tgccctttcg ccaggtcagc ggcgtggacg gcaatcttat aggtgataag
    17461 cccctgctta acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca
    17521 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17581 atagcccggc gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17641 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc
    17701 ttcaatcatc acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg
    17761 cagctcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17821 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17881 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17941 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    18001 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    18061 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    18121 tgtaccgatc ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    18181 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    18241 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    18301 caggaaattg cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18361 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18421 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18481 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18541 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18601 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18661 gcgcacggta agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc
    18721 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18781 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18841 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18901 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18961 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagtcaag
    19021 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    19081 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    19141 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    19201 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    19261 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    19321 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19381 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19441 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19501 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19561 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19621 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19681 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19741 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19801 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19861 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19921 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt agtacaacag
    19981 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    20041 caactggccg gacgaggcgt acaactggcg gagttttacc gatcgcataa atactgtggt
    20101 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg cagccattgc
    20161 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    20221 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    20281 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    20341 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20401 ttaatgaccg cgtttatgac ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20461 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20521 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20581 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20641 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20701 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20761 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20821 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20881 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20941 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    21001 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    21061 tggacgctgg cgacctacat ggtggaaggc ggcagcagca aagcgttcac cgtgatcaaa
    21121 aaaatgatgt atgccgatcc gcaggcgctg cacgctctac tcgataaact ggcgaaaagc
    21181 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    21241 acctggggcg gtgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    21301 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21361 accaaaggcg gcggacagtg gctggaagcg atggcagaaa ccggttgcga tgcgttgggc
    21421 ctcgactgga caacggatat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21481 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21541 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21601 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21661 tctgaacaat atcaccgcta aggagtgatt atggatctcg cgtcattacg cgctcaacaa
    21721 atcgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc accggatctg
    21781 atcgccggag tcgatgtcgg gtttgagcag ggcggagaag tgacgcgagc ggcgatggtg
    21841 ctgctgaaat atccctcgct tgagctggtg gagtataaag ttgcccgcat cgccaccacc
    21901 atgccttaca ttccaggttt tctttccttc cgcgaatatc ctgcgctgct ggcagcgtgg
    21961 gagatgctgt cgcaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    22021 cgccgtcttg gcgtcgctag ccattttggt ttgatggtgg atgtgccaac gatcggcgtg
    22081 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    22141 ccactgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    22201 ccgttgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    22261 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    22321 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    22381 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    22441 ctggaaagct ggcagcatgt cactttcatg gcttgcttat gcgaacgcat gtaccccaat
    22501 tacgccatgt tctgccagca aaccggtttt ggtgatgggc aaatttaccg tcgtattctc
    22561 gatctcatct gggaaacgct gaccgttaaa gacgcaaaag taaatttcga cagccaactg
    22621 gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg
    22681 gcaatcgatg cctgcgtggc gttaagtgaa ctggtccatt cgcgtttgag tggtgaaacg
    22741 ctcgaacacg cggtggaagt gagtaagacc tccatcacga ccgttgcgat gctggaaatg
    22801 actcaggctg gtcgcgaaat gagcgatgaa gagcttaaag aaaacccagc tgtagagcaa
    22861 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22921 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22981 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    23041 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    23101 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    23161 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    23221 gcagaactgt ccaaaactca ggctaaagct gctctggagt ccactctggc tgcaattact
    23281 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    23341 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    23401 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    23461 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    23521 tcattgatgc tggctggcgc tctgcttctc actgcctgta gtcataactc ttcacttcct
    23581 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    23641 agcggcgata atgtgcatct gcttgccgtg tttagcccgt ggcgcagtgg cgataccacg
    23701 acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc
    23761 aaaccgccgg tgaatattcg cgcgcgtttt gacgatcgcg gtgatctgag ctttatgcaa
    23821 cgtgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23881 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23941 caggggcgct ggcatgcgat ggaacagacc gtgaccacct gcgaagggca aaccattaaa
    24001 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    24061 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt agtggcaaac
    24121 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggcccatac
    24181 ccatatgacc gccaccaccg cagccgccgt agcccatgcc cattccggct ccgcgcggaa
    24241 tacccgcttc agccatcgcg atatctcgtt tcacccgtaa ctcatctaac gactgacgca
    24301 aattctccat ctctttggcg accgcgttaa ttttgctgct atccggtggg ttcgcggcta
    24361 acagggcatt gtattcataa cgcttcgtca ccagttgctg ttgcagtgcg ctgctttgag
    24421 cgtaaaagtc attatggatt ttctgccacg ctgtctgttg ttcgctggtc aaaggcgcgg
    24481 cattttgctg ccacataccg tgtccgccgt gagcaaatgc agatgtcgat cccatcgcca
    24541 ttgctgaaag cgccatcatt accagggcaa ttttcgtgtt ccgtttcatg gttaatcctc
    24601 cagtggttgt cttacttcgg gtattgcatc ttccgtgcca acgatgaaac gctgatatga
    24661 cgggtaatct ggcatgataa acgagtaaaa atgactcgcc tgctgcgggt agcgagtcat
    24721 ttttactcat tgaaacttaa gcctttgtgt tacagcgcag ggtaagcgct gataaaagat
    24781 ggcatgattt ctgctgtcag aaagggatga gcaggcaaag aagaagatgc gttttatgca
    24841 acgttctaaa gactccttag ctaaatggtt aagcgcgatc ctccccgtgg tcattgttgg
    24901 gctggtaggg ttgtttgcgg tgacggtgat tcgcgattat gggcgcgaga ctgccgccgc
    24961 cagacaaacg ctgctggaaa aaggcagtgt acttattcgc gctcttgaat ccggctcgcg
    25021 cgtcggcatg gggatgcgca tgcatcatgc gcagcagcag gccttactgg aagaaatggc
    25081 cgggcagcct ggagtacgtt ggtttgcggt cacggatgaa caaggaacaa tcgtgatgca
    25141 tagcaactcc ggcatggtgg gaaaacagct ttattccccg caggaaatgc agcagttaca
    25201 tccgggagat gaagaagcgt ggcggcggat cgatagcgca gacggcgagc ctgttctgga
    25261 aatttatcgc cagtttcaac cgatgtttgc tgctggaatg caccggatgc gccatatgca
    25321 gcagtatgcc gcgacaccac aagcaatttt catcgctttc gacgccagta atattgtgag
    25381 tgccgaagat cgtgagcaga gaaacaccct gattatcctc ttcgccctgg cgacggtctt
    25441 gctggcaagc gtattgtcat tcttctggta tcgccgctat ttgcgctcgc gccagcttct
    25501 acaagatgaa atgaagcgca aagagaagct ggtggcgctg gggcatcttg cggcaggcgt
    25561 tgcccacgaa atccgtaacc cactttcctc gattaaagga ctggcgaaat actttgccga
    25621 gcgcgcgcct gcagggggag aagcgcatca actggcgcag gtgatggcga aagaggccga
    25681 ccgtttaaac cgcgtggtaa gcgagctgct ggaactggtt aagccaacgc atctggcttt
    25741 gcaggcggtg gatctcaaca cgctgattaa ccactcatta cagctggtaa gccaggatgc
    25801 gaacagccgg gagatccagt tacgctttac cgccaacgac acattaccgg aaattcaggc
    25861 cgatccggac aggctgactc aggtcctgtt gaatctctat ctcaatgcta ttcaggcgat
    25921 tggtcagcat ggcgtgatta gcgtgacggc cagcgaaagc ggcgcgggcg tgaaaatcag
    25981 cgttaccgac agcggtaagg gaattgcggc agatcagctt gatgccatct tcactccgta
    26041 cttcaccact aaagccgaag gcaccggact ggggctggcg gtcgtgcata atattgttga
    26101 acaacacggt ggtacaattc aggtcgcaag ccaggaggga aaaggcgcaa cgttcaccct
    26161 ctggcttccg gtcactatta cgcgtaaggg cccacaagga tgacgcacga taatatcgat
    26221 attctggtgg tggatgatga cattagccac tgcactattt tgcaggcttt actgcgcggc
    26281 tggggctata acgtcgcgct ggcgaacagc gggcgacagg cgctggagca ggtgcgggaa
    26341 caggtttttg atcttgtgct ttgcgatgtg cgaatggcgg agatggacgg cattgccacg
    26401 ctaaaagaga tcaaagcgtt aaatccggcg attccggtgc tgattatgac cgcatactcc
    26461 agcgtcgaga cggcggtaga agcgttgaaa accggggcgc tggattatct catcaagccg
    26521 ctggatttcg ataacctgca ggcgacgctg gaaaaggcgc tcgcgcatac gcacagtatt
    26581 gacgctgaaa cgcctgcggt gtctgccagc cagttcggta tggtcggtaa aagcccggcg
    26641 atgcaacacc tgctcagtga aatcgccctc gtcgcgccat cggaagccac ggtgctgatc
    26701 cacggcgatt ccggcaccgg taaagagctg gtcgccaggg cgattcacgc cagtagcgca
    26761 cgtagcgaaa aaccgctggt aacgctcaac tgtgcggcac tcaacgaatc cttgctggaa
    26821 tctgaattgt tcggtcacga aaaaggggcg ttcactggag ccgatagacg ccgggaaggg
    26881 cgctttgttg aggcggacgg cggcacgctg tttctcgatg aaattggcga tatctcgccg
    26941 atgatgcagg tgcgtctgct gcgtgcgatt caggagcgcg aagttcagcg tgtcggtagc
    27001 aaccagacta tctcggttga tgtccggttg attgcggcga cccatcgcga tcttgccgca
    27061 gaggtgaatg ccgggcgttt tcgccaggat ctctactatc gcctgaatgt ggtggcgatt
    27121 gaagtaccat cgctgcggca acggcgggaa gatatccctc tgctggctgt ccattttctt
    27181 cagcgctttg ccgagcgtaa tcgcaaggcg gtaaaaggct ttacgccaca ggcgatggat
    27241 ttgctgatcc actacgactg gccgggaaat attcgtgaac tggaaaacgc ggtggaacgg
    27301 gcggtggtgc tgctgaccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc
    27361 ggtacgccga tcccactggg gcaaagtcag gatattcagc cgctggtgga agtcgaaaaa
    27421 gaagtgattc tggcggcact ggagaaaacg ggcggcaaca aaaccgaagc cgcccgtcag
    27481 ttagggatca cgcgcaaaac gctattggca aaactgtcgc gttagttctg ctcgcgttcg
    27541 atagcgcgcc agccgatatc tttccggcag aagcagtcgt cccagtgaat atcggtcatt
    27601 aaggcataag cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt
    27661 acgcgcccgc cgctggtcac tacctgctcg ttatccgcca gtttcgtgcc cgcgtggaac
    27721 actttgccgc ctgccacttc ttccagcggc aggccgtgga tcacatcgcc ggtgcggtaa
    27781 tcgcccggat atccacccgc agccatcacc acgccgagag aagcacgctc atcccactct
    27841 gacgttttct cgtccagttt gccttcacag gccgccaggc agagctcaac cagatcggac
    27901 ttcatgcgca gcataatcgg ctgggtttct ggatcgccaa agcggcagtt aaattcgata
    27961 accttcggat tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg
    28021 ttgccttccg ccgccatgcc tttcacggtt ggccagatga tacgttccat ggtgcgctga
    28081 tgaacttcat cggttactac cggcgcgggg gagtaagcgc ccatcccgcc cgtgtttggt
    28141 ccggtatctt tatcgcctac gcgtttgtga tcctggctgg tggccatcgg cagcacatgc
    28201 tcgccgtcta ccatcacgat aaagctcgct tcttcgccgt cgaggaactc ttcgataacg
    28261 atgcgatgac ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcg
    28321 gcttcttcca gcgtcatcgc cacgataacg cctttcccgg ctgccagacc gtccgcttta
    28381 atgacgatcg gcgcaccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag
    28441 ttctggtatt ccgctgtagg gatcttatgg cgggccagga aatctttggt aaacgctttc
    28501 gaaccttcca gttgcgcggc acctgcggtt gggccgaaga ttttcatccc ggcggcgcgg
    28561 aaggtatcga ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc
    28621 ttttcgtttt gtgcgaaatc caacagcgcc gggatatcgg tcacgccgat agcaacgttt
    28681 tgcagcgcgg gttccagcgc agtgcctgca ttacccggag caacaaaaac agtttcaacc
    28741 agcggcgact gcgccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact
    28801 aatactttca tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg
    28861 caataccgtg ctcgtcggcg gcggcaatca cttcgtcatc acggatagaa ccgccaggct
    28921 ggattacgca ggtcacgcct gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga
    28981 acgcgtcaga agccatcgag gaacctttca cttccaagcc ttcatcggca gctttaatac
    29041 cggcgatttt cgcggagtac acgcggctca tctggcccgc gccaatcccg atggtcatat
    29101 tgtttttggc atagacgata gcgttggatt tcacgaactt cgccactttc cagcagaaca
    29161 gcgcatcacg cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg
    29221 caccgaccat gcccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat
    29281 caagacccgg aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg
    29341 cggcggtgat tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact
    29401 gacgagaaat gatggcctgt gcggtttccg catccagctc gcggttaaag gcaatgatgc
    29461 cgccgaatgc ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat
    29521 tgccgatagc cacgccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga
    29581 actctttcac gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt
    29641 taccctgaac ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga
    29701 aggcagcctg ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca
    29761 ggttcagcgt gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg taagccggaa
    29821 ccatgctgcc gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt
    29881 tgatggcgag gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt
    29941 taataatggc gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag
    30001 cggcggagcg caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg
    30061 agcagccttc acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga
    30121 taggctggat ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc
    30181 caccatgtac tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg
    30241 tgtaatcgga aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc
    30301 ctgtagacag cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg
    30361 ctttgtcaga aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc
    30421 ccctggattt gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac
    30481 gcccctaacg gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc
    30541 tcgcgaattt ttctctttca atggtgatca caattttgac agtggttacc gtgggaaaaa
    30601 tacagaaatt acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt
    30661 tttgctgggg aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga
    30721 gaaatcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc
    30781 cggttcggtt gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta
    30841 atatacgcca cctcgcgaca gtgcgctaaa
//
LOCUS       DAJTVJ010000041        30731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000041 DAJTVJ010000000
VERSION     DAJTVJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.041"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            228..473
                     /locus_tag="OO448_001808"
     CDS             228..473
                     /locus_tag="OO448_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337029.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            470..892
                     /locus_tag="OO448_001809"
     CDS             470..892
                     /locus_tag="OO448_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6337030.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1552..3419
                     /locus_tag="OO448_001810"
                     /pseudo
     CDS             1552..3419
                     /locus_tag="OO448_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            3678..3959
                     /locus_tag="OO448_001811"
     CDS             3678..3959
                     /locus_tag="OO448_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337031.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            4599..4919
                     /gene="clpS"
                     /locus_tag="OO448_001812"
     CDS             4599..4919
                     /gene="clpS"
                     /locus_tag="OO448_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6337032.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            4950..7226
                     /gene="clpA"
                     /locus_tag="OO448_001813"
     CDS             4950..7226
                     /gene="clpA"
                     /locus_tag="OO448_001813"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6337033.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(7632..7719)
                     /locus_tag="OO448_001814"
     tRNA            complement(7632..7719)
                     /locus_tag="OO448_001814"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7683..7685),aa:Ser,seq:gga)
     gene            complement(7973..8191)
                     /gene="infA"
                     /locus_tag="OO448_001815"
     CDS             complement(7973..8191)
                     /gene="infA"
                     /locus_tag="OO448_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6337034.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(8476..9180)
                     /gene="aat"
                     /locus_tag="OO448_001816"
     CDS             complement(8476..9180)
                     /gene="aat"
                     /locus_tag="OO448_001816"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6337035.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(9222..10943)
                     /gene="cydC"
                     /locus_tag="OO448_001817"
     CDS             complement(9222..10943)
                     /gene="cydC"
                     /locus_tag="OO448_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6337036.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(10944..12710)
                     /gene="cydD"
                     /locus_tag="OO448_001818"
     CDS             complement(10944..12710)
                     /gene="cydD"
                     /locus_tag="OO448_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6337037.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(12833..13798)
                     /gene="trxB"
                     /locus_tag="OO448_001819"
     CDS             complement(12833..13798)
                     /gene="trxB"
                     /locus_tag="OO448_001819"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6337038.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            14343..14837
                     /gene="lrp"
                     /locus_tag="OO448_001820"
     CDS             14343..14837
                     /gene="lrp"
                     /locus_tag="OO448_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6337039.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            14972..19039
                     /gene="ftsK"
                     /locus_tag="OO448_001821"
     CDS             14972..19039
                     /gene="ftsK"
                     /locus_tag="OO448_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6337040.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            19194..19805
                     /gene="lolA"
                     /locus_tag="OO448_001822"
     CDS             19194..19805
                     /gene="lolA"
                     /locus_tag="OO448_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6337041.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            19816..21159
                     /gene="rarA"
                     /locus_tag="OO448_001823"
                     /gene_synonym="mgsA"
     CDS             19816..21159
                     /gene="rarA"
                     /locus_tag="OO448_001823"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6337042.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            21250..22542
                     /gene="serS"
                     /locus_tag="OO448_001824"
     CDS             21250..22542
                     /gene="serS"
                     /locus_tag="OO448_001824"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6337043.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            22781..25225
                     /gene="dmsA"
                     /locus_tag="OO448_001825"
     CDS             22781..25225
                     /gene="dmsA"
                     /locus_tag="OO448_001825"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCR6337044.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            25236..25853
                     /gene="dmsB"
                     /locus_tag="OO448_001826"
     CDS             25236..25853
                     /gene="dmsB"
                     /locus_tag="OO448_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6337045.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            25855..26718
                     /gene="dmsC"
                     /locus_tag="OO448_001827"
     CDS             25855..26718
                     /gene="dmsC"
                     /locus_tag="OO448_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6337046.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(26754..27380)
                     /locus_tag="OO448_001828"
     CDS             complement(26754..27380)
                     /locus_tag="OO448_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6337047.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            27695..28843
                     /locus_tag="OO448_001829"
     CDS             27695..28843
                     /locus_tag="OO448_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6337048.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            29053..30483
                     /locus_tag="OO448_001830"
                     /pseudo
     CDS             29053..30483
                     /locus_tag="OO448_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(30484..>30731)
                     /locus_tag="OO448_001831"
     CDS             complement(30484..>30731)
                     /locus_tag="OO448_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6337049.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         7766 a         7434 c         8197 g         7334 t
ORIGIN      
        1 attatagggt gaacagttat ttatgaactg ttcaccaaac tattcactgt tcaccttttt
       61 gattctttat tgagctttaa gggtgaacag tggtgaacag ttggtgaata gtttttgtga
      121 aactgttaac ccattaacat catgaataaa aagggaaaat tgcaaaaggt gaacaggtga
      181 agggttaaaa tgcaaaaatt ttattttatt gctgtgaggt aaagcctgtg acaacgaagc
      241 acgccaaaaa accacaatct cacgcccttg atttgacaga acactggctg agggtggcga
      301 taaaaatcat cgaccgcaac gccggggaag gatacgcgaa agcacatccc gaactgataa
      361 gcgcattcat gaccacgacg gcggcaaact ttgccacgct gacagaacgg gagattgccg
      421 aagcggaaca ggtgacaacc atcaacgtta aaaccggaga gcagacagca tgacagcaca
      481 gatagcggct tacgggcggc tggtggctga cccgcagtta aagaccacca gcaagggtac
      541 gcaaatgacg atggcgagta tggcggttcc cctgccgtgc agccaggcag atgacggaac
      601 ggcgacgata tggttatccg tcctggcgtt tggcagacag gctgacgcac tggcaaagca
      661 ccagaaaggc gagctggtga gcgtggcggg taacatgcag gtaagccagt ggacaggcca
      721 gaacggagaa acgcggcagg gctggcaggt tatcgcagac agtgtaatca gtgccagaac
      781 ggcgcgaccg ggcggtaaaa aaggccaaca ggggcaggcc actgatgcac tgaacagggc
      841 caaacaacag gcggggaatg atgatccgta cggggataac ataccgtttt aagcaacgag
      901 tgacagaagc cggagcaatc cggctttttt gtaggtactc ctggtggggg tggcctgtcc
      961 acggggcggg cggttcgcgg aaaaaggcgt attttttgat ttttatgtca ccatcaccac
     1021 cagtttaagt tattgatata tggcaaaata aaaattttta gtgtcgaatt aggttgtttt
     1081 ttgttcgacg ccggaacgtt cccgaaagca tttacaaaaa acaggcgcaa aaaaaagcgc
     1141 cccgatggtg ccaccggagg cgctttgtca gcgacaaagg atactgccac taccgaaatg
     1201 atgagtctta attgtgctgg cgtggcaata attagtcaat tcttttgctg gtatgacaaa
     1261 aatcagaaaa cacataatct gatttttaga tagaaaatga tttatttacc agttttatcg
     1321 atcaataatg atgcccgtta atcataacgg aggcagactt atgccagaga acaacagcag
     1381 aaaaccggat aaaagtgcaa cagtgcacat agacgccgga actatggaga agctcgaacg
     1441 ctatcagcag ttcattaaaa gtcatcaccc tggaatgcca gtacccacga aagggcagat
     1501 tgcgagaagc gcggttgaat actggtacag ggcaacgtta ggagcctggc tatgaaaaca
     1561 tggttttcca ttaaggctat ggctgatgtt gtacatgtac gcatttatga cgagatcggc
     1621 gggtacggtg taaaagcatc ggcacttact gacgagatca acgcgtgcgg taatgtgcct
     1681 gaaatccatc ttcgcatcca ttcacctggt ggcgacatct ttgaagggct ggctatctat
     1741 aacgccctga aaaatcatcc ggcaaagaaa attgtacaca ttgaaggcat ggcggcttct
     1801 atggcctcgt ttattgccat gtgtggcgat cacatcgtta tgcctgaaaa cgcgatgatg
     1861 atgatacatg ccccccgtgg tgttactgcc ggagtgtcgg gcgacgttcg ccgctttgct
     1921 gacctgatgg acaagctggg cgacacgatg gcggaaacct acgccggaag aacgggcagg
     1981 agcaaacagg aaatcaccgc catgatggag gcggaaacct ggatggatgg caatgagtgt
     2041 aaggctaatg gcttcgcaga tgaggttata cccgcgatta cagcaatggc cagaattgaa
     2101 tcaaaacgaa tcggagattt ttcgaatatg ccggaaaata ttaaaagcat gatcagccag
     2161 aaaactggca gcggagaaca ggaacgactc aacgggatac gtgaattgtt tggcacgttc
     2221 aatggaagat ataacgacct ggctataagt tgtcttgctg attcaggctg tagcgttgag
     2281 gatgcacgcg aacgcctttt actcactatg ggtaaagaat cgacaccgac aaacaaaacc
     2341 acccccgcaa atctttacta cgcgtacacg gataacggca acataaccgg cgatgcaatg
     2401 cgtcaggggc ttaatgcgcg tctgggacat gaacaggcag aacgcggcaa cccttacgcc
     2461 atgatgagcc ttttcgatat ggcacaggca tcattaaccc atcgcggcat aagcacaggc
     2521 agctacagca cacgctcgca gatagtaaac gcggcattca cccacagcag cagcgatttt
     2581 acttatatcc ttgctggtgg tgctgaaaaa tccgtgaaca cagcggcgaa acgttccgcc
     2641 agtggacgaa aaaaggttca ttgtcaaact tccatgaggc acgtcgtgtt ggcctgaatg
     2701 gcttctctac attaaacaaa gtgccggaag gcgcggaata taaatacatc accaccagtg
     2761 accgtggcga accaatcgcg ctggcaactt acggaaatat tttcagcatt acccgacagg
     2821 cgattattaa cgatgacctg aatcagttaa cagttatccc acaggcaatg gggcgagcag
     2881 ccgccagaac ggtgggtaat ctggttcatc tggtgctgac aggaaacaca aagctttctg
     2941 acggtatacc gctgttcagc aaaaaacacg gaaatgatat tgataacgga gtcagcacta
     3001 cagggcttgg cattgcacgc cgtgcaatgc gcacacagag ggatgaaaac ggtgaggtgc
     3061 tgaatattgt gccaaaattc cttcttgttc ctgcggctct ggaagacaga gcgttgcaaa
     3121 tggttaactc aaccacgcct ttcggtgccg atacaaacag tggttcatat aacccgtacc
     3181 acaaacaact ggatattatt gtcgatcccc gccttgatga agtcagtgat aagcaatggt
     3241 acatgctttc agcacaggga acggacacaa ttgaggtggc ttatcttgac ggtaatgacg
     3301 agccttacct tgagcagcag gagggcttta ttgttgacgg tgtggcctgg aaggtccgca
     3361 ttgatgctgg cgttgctgct ctggattatc gcggaatggt cagagcgatt tcttcatgag
     3421 aacaaggcgg cagcagccgc cttttttgcg ggtcctcctg gtgggggggg cctgtccacg
     3481 gggcgggtcg gcgcggaaaa aagcgcattt ttgtggtttt atcgtcatca tcatcatgtg
     3541 tgtaacctgt tgtttttaat gtagttgatg caaaaaagat gatgattgtg gttaattttt
     3601 gttcgacatc ttttagcgtg acagattctt tacaaaaaat ctgagcttgt tttcttaacc
     3661 agcgcgatgg gggcacaatg acagaagccg aaatactggg attaatccgc cgcgtcgccg
     3721 gaatcagcca gcaggttgac gaacaggcca tgcaaccgga cagcgtgacc gctgaaaatt
     3781 atgcgcgtgt ggtggatgag gtaatgcgcc gtgacggtat tgagcttaac ggcgtggata
     3841 tgcgcaacat acgaaccaga gttcttgagt tgctggcata ccgtcgccgt tctcaacaac
     3901 ggagggagag cgcgaaaaat acttaccagt ggaggaagcc ggagcactta cggcggtaac
     3961 tggttgatat taccgaaagt gcaaaaatgc acttagcaac tggtggcgag ttgcagatct
     4021 gcaactcgac tatgaaacta cggaaactac ccgtagtttg ggtagtaaga gtaacaccca
     4081 gattttgggg cttactcgcg atacccaaat aaagggtatc ggtggaagaa atatcgtttc
     4141 tcatatgtga gtaccgaggg cggaattccg ccttcggtta cttattgtgc gcatgcacag
     4201 ggaggggcgg gtcaaatctc tgtaccctga cgtcttccgg actgccagcc ccatcgattt
     4261 tttatacccg cgaaaaatga aaaacgcttc acgctggtgg gcctgatgcc gataactggt
     4321 ttcatgactg atagccggaa aatgataacg attaaagcgt gttgatcttt gttcgtggtt
     4381 gttcgatatt gttcgtggaa cggtgtagtt aatggtgtag ttaattttac tgtttttgac
     4441 gagcttattg tttgcaactc aatgaaaaat aaaggctaat aagccaaatg ctataaatga
     4501 tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca gcggcagggc
     4561 gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg aacgactggc
     4621 tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg ccatctatgt
     4681 ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt gacgtgttac
     4741 aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct gttcactacc
     4801 aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa gtggcgatgg
     4861 tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa aaagcctgaa
     4921 tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa ctcagtttaa
     4981 atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc gagcacttgt
     5041 tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt tctgtggatt
     5101 tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc gttctgcctg
     5161 ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta ctgcaacgtg
     5221 cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac gttctggtcg
     5281 ctatctttag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat gaagtcagcc
     5341 gtctcgatgt ggtgaatttt atctctcatg gcacgcgtaa agacgagccg acacagtctt
     5401 ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa cgtatggaga
     5461 atttcacgac gaacctgaat cagcttgcgc gcgtgggcgg aatcgaccca ctgattggtc
     5521 gtgagaagga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa aacaacccgc
     5581 tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc ggaaggtctt gcctggcgaa
     5641 ttgttcaggg cgatgtgccg gaagtgatgg ctgactgtac gatttactct ctcgatatcg
     5701 gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt aaagcgttgc
     5761 tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc cacaccatta
     5821 tcggtgcggg ggcagcgtct ggtggtcagg ttgatgcggc taacctaatc aaaccgttgc
     5881 tctccagcgg taaaattcgt gtaattggtt cgacaaccta tcaggagttc agcaacattt
     5941 tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact gaaccgtcaa
     6001 tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg caccacgacg
     6061 tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa tacatcaatg
     6121 atcgtcatct gccggataaa gcgattgatg ttatcgacga agcgggcgct cgcgcacgcc
     6181 tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag tccgtggtgg
     6241 cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat accctgaaaa
     6301 acctcggcga tcgcctgaaa atgctggtct tcggtcagga taaagccatt gaggcgctga
     6361 ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg gtcggttcgt
     6421 tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag ctttcgaaag
     6481 cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc cataccgtca
     6541 gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt ttgctgaccg
     6601 atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag aaagcgcatc
     6661 cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc gataacaacg
     6721 gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac caccaacgcc ggggtacggg
     6781 aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat gcgatggaag
     6841 agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt atctggtttg
     6901 atcatctgtc aaccgacgtg atccatcagg tggtggataa attcatcgtc gagttgcagg
     6961 ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt aactggctgg
     7021 ccgagaaagg ttacgaccgg gcaatgggcg ctcgtccgat ggcgcgtgtc atccaggaca
     7081 acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac ggcggtcagg
     7141 tcaccgtcgc gctggataaa gagaaaaatg agctgactta cggattccag agtgcacaaa
     7201 agcacaaggc ggaagcagcg cattaatcta attgttaggt aggttggtga agtccgtaat
     7261 ctcgaaagag gttacggact ttttgtttat ggggtggaac ggtgaaaacc ctatttttgg
     7321 aggtgaaggt aatttgttga taattagtgc tgctggaaaa ggtaaggata aaaaagggtg
     7381 acagcaggaa aatgagatgg ttttgcttta ttaacaacgg actaaacgtg tagtatttga
     7441 gttcactgcc gtacaggcag cttagaaatc gacggggtgc ggtaaaacct ttgcgaacgc
     7501 gttcactgcc gtacaggcag cttagaaatt cacaggtaac atactccacc cgcccaccat
     7561 gttcactgcc gtacagaaag ataaaatgcg aaaaaaaagc tcgcactttc gtacgagctc
     7621 ttctttaaat atggcggtga gggggggatt cgaacccccg atacgttgcc gtatacacac
     7681 tttccaggcg tgctccttca gccactcgga cacctcacca aattgttttg ctaccaaacc
     7741 tcatgggtgg caacggggcg ctactatagg gagttggagt aaaacggtca agaagaattt
     7801 taatgataat tattgtttgc tcatactgta aacaagttgt gcagtatatc tacatcgaga
     7861 caagttacgg acttatactt ccaaagtact tcatacatat cacaaaataa aaaggccggt
     7921 taaaccgacc ttttactcgt tctttctctt cgcccatcag gcggtaaaac aatcagcgac
     7981 tacggaagac aatgcggcct ttgctcaggt cgtacggggt cagttcaaca gtcactttgt
     8041 cgcccgtcag gatgcggatg tagtttttgc gcattttacc ggagatgtgt gcagtaacca
     8101 cgtgaccgtt ttctaactct acgcggaaca tggtattagg caacgtttca agaacggtac
     8161 cttgcatttc aatattgtct tctttggcca tctaatcctc tggggtatca ctaccgtaat
     8221 ttgaaccggc aagataatgc cgaagttctg taaataagta aagatttgcg cgctaaatcg
     8281 caacaaacag gttcggcaca ttactccgaa aacacacggc taagccgcac caaaagcgca
     8341 acgtataagg gagcggtgag ataaacgatg ggcgttacct gacgcgaaaa attccttatc
     8401 ggcagcgggg taatgagcat aaccaactct gcgaccgcaa ttataacact ctggggagaa
     8461 atgtgccgaa aacattcatt tttgtggtga aaacaagcat cgtggcaccc agaaattatt
     8521 cggcaatcgt ccgaggcgca tttgattgag ataattcaga taatcccgac ggggaatttc
     8581 gcaggcacca agcgatgctg tgtgatcgtt aaggacctgg cagtcgataa gcttaccgcc
     8641 atgactgata aattcctcac agaataccag aagcgccgtt ttagacgcat tttccatccg
     8701 gctgaacatg gactcgccac aaaatagcgt tccctgggcc acgccgtaca taccgccgac
     8761 aagctcatct tcatgccaga cttcaatgga gtgggcatga ccgagttcgt gaaggcgatg
     8821 gtaggcttcg accacgccac gcgtgatcca ggttccttct tcgcgatcgc tggcacaacc
     8881 ttcaatgacc tgaccaaaag cgtaattcat cgtgacacga tagggcgagc gtttatgaaa
     8941 tcgcttcata ctacggctga tatgcagtga ttcaggccaa agcaccgcgc gaggatcggg
     9001 cgaccaccag aggatggggg cgcctggaga aaaccacgga aaaataccac gctggtaagc
     9061 cattaacagg cgcgcagggc taagatcgcc ccccagagcc agcaggccgt taggctcacg
     9121 taatgcgcct tccggggaag ggaaggctat tgaatggcga gaaagctgaa ccaggcgcat
     9181 gaccgcaaaa ctccacgcaa gtcggatcgt tcaataatag cttacaaacc ctgcttaaac
     9241 tggtaataac gcccctgtct ggcaagcagt tctgcgtgag taccttgctc aataatttgc
     9301 ccgttgtcca tcactattat ttgttggaaa cgagagagtc cgcgaagtcg atgggtgacc
     9361 attaacaccg ttttctcacg catcatttct gcaagcaatt caaggatctg gctttcggtt
     9421 gtggcatcta agccttcggt aggttcatcc agcaacacca gtggcgcatc atgtaacagc
     9481 gcacgggcga tagccagacg gcgcagttca ccaccggaga gctggcgtcc gccttcacct
     9541 aaccaactgt tgagacctgc gtcctcgagc agcttctcca gaccaacgcg acgcaagatc
     9601 tccgacagag cctcatcact actgccaggc gaggcgagta acagattatc acgcagcgtg
     9661 gcgctaaaca gatgcactcg ctgaggaaca acgctgatgg tctgtcgtag agccgcttca
     9721 ttcaggctgg ctatggggct atcgttaagc aaaatctcgc cctgttgcgg gtcccaagcg
     9781 cgggtcagca gttgtaacag tgttgatttg ccgcatccgg ttcgcccgag aatcgcaata
     9841 tgttccccgg cgtttacctg aagagaaatc cctttaagtg cctgttggga ttgctccgga
     9901 taagtgaact gaacatcccg taatgtcagc gaaacgcgat cggcaacacg agtttgggta
     9961 tcagggaagg tgacttccgg tttttgatcc gttaaatcag tgatccgaac ggcagaggca
    10021 atgacttgcc ccagatgctg aaatgcaccc gttactggtg ccagtgcttc aaacgcggct
    10081 aacgcgcaga agacaaacag ggcaattaac gcgccgggtt gagcattgcc gccaacgccg
    10141 ccagacgcca tccacagcat caggatcacc gctaacgcgc caatgagcag cattatcgct
    10201 tgcgacaatg cggtcagttc agattgacgg cgttgcgctt ccagccattg aatttctgta
    10261 ttctctagtt gcgtgcgata acgatcgctg gcaccaaaaa tggtcagctc agcttgccct
    10321 tgcagccagg ccgtcagttg ttggcgatac tgtccgcgaa gatgagtcag attttgcccg
    10381 gtgctttttc ccgcacgata aaacagcggt ggcatcagga aaagcgtcag taacataatg
    10441 ccgcccagcg taaaggcgag ggtgaaatca aggaaactta acccgactgt caccaccata
    10501 atcaccacaa aagcgcccac cagcggcgag ataacgcgca ggtaaagatg atcgagcgta
    10561 tcaacatccg ccaccacgcg attgagcaat tcgccctgac gatagcgcgc cagtccggca
    10621 ggggagaggg gcaacaattt gctgaaggtg taaatgcgca gatgctgcaa cacgcggaaa
    10681 gtcgcgtcgt gacttaccag acgttcaaaa taacgcccgg cagtacgggt gattgcggca
    10741 ccacgcacgc ccgcagcggg tagcatatag ttgaagctgt acagtccggc accccccgca
    10801 accgctgagg ccgagaggaa ccagccggaa agtgtcaaca gaccgatact ggcgagcagc
    10861 gtcacaattg ccagcacaat accaagactt aacatccatt tatgacgttt atacagtgcc
    10921 agatagggta gcaaagcgcg catttaaatc tcctcctgac gatgggccag taatgtggcg
    10981 aatgggccac cagccacact taattccgcg taacgtcctt gctcaataat ctgaccatcc
    11041 tgcattaccc aaatgacatc ccagtcagca agatcttcta actggtgggt gaccattaac
    11101 gttgtctggc gcagagaggc ggcattcagc gcctccatta cgcgctgttc actgtgagca
    11161 tcaaggctgg cagcgggttc atccaacagt aatagcgaac agggatttag taacgcacgg
    11221 gccaccgcca cgcgctgcgc ctgccccacg gaaaggcggg cagcctggtc gccaacaggc
    11281 gtatcaacgc cttgtggcag gagcggtaga aactcgctga cccaggcgtt atccagcgct
    11341 gcttgtaatt catgttcgct ggcatcaggt cgcgccagta gtacgttatc ccgtaatgtt
    11401 gctgccggta attgtggatt ttgcccaacc caggagagat gtttacgcca tgattctggt
    11461 gataaatcgc gtaattctat cccgttgatt cgtagcgatc cctgatatga gagaaaacca
    11521 gaaagcgcgt tcaacagtga acttttacct gaaccgctgc gaccaaccaa cacggcacgt
    11581 tggcctgctg gcaaagtaaa gttcagcggt ccggccagcg ttttaccttc cggcgacgtg
    11641 ataaacagat cctcggcctc aatggtcacc ggatcggtcg atgctaattc cacctcgcca
    11701 cgctgcggat gggcgagcgg ggtttccata aacgttttca gactgtcagc tgcaccaaca
    11761 gcctgggctt tagcatgata aaacgtaccg agatcgcgta atggctggaa aaactctggc
    11821 gcaaggatca gggccagaaa acccgcagcc agcgtcacgc cagtatcgta gtgaccaaaa
    11881 tccagctcgc cgagatagga aaaaccaaag tagaccgcca ccagagcaat tgacagcgag
    11941 gtaaaaaatt cgagaatgcc ggaggataaa aatgccagcc gtagcacttc cattgtccgt
    12001 tggcggaaat cttccgaagc agaacgaata ctttcaattt cagcttcacc acgaccaaaa
    12061 atacgcaatg tttccatgcc gcgcaggcga tcgaggaaat gcccacttaa gcgagcaaga
    12121 gcgagaaagt tacgtcggtt agcatcggca gcccccattc caaccagcgc cataaacaac
    12181 ggaattaacg gtgcagtgcc cagcagaatg agcgccgcag cccagtttga ggggaagatt
    12241 gccaccacaa tcagcaacgg caccgacact gccagcgcca tttgcggcag gtagcgtgca
    12301 tagtaatcat gcatatcgtc aatttgctcg agtaccagcg tcgcccagct ccccgcaggt
    12361 ttaccctgaa tccacgctgg ccctgcttgt tgcagacggt cgagaacctg acggcggatg
    12421 gcaaagcgga tatgctgccc ggcgtgataa cccacccgtt cgcgtaacca gaccgcccat
    12481 gcgcgcagta caaaggtcag aaccagtaac gtaaagggaa gcagcagggc ttcacgggga
    12541 atattctcca taatcatatg ttgcagaatt cgcgccatga accaggcctg ggcaatgatc
    12601 aatatgccgc ttacaaagcc cagcagacga gaaatattca gccaacgttg ggagatgacg
    12661 ctttgctgtt ttaaccagcg ggttaactct ttttgacgag atttattcat tgcacgctta
    12721 gcaggtgagt tatcagaatt atttgcagag caatgttaca acggggaaaa aataaaggcg
    12781 acccatagtc gcaaggtgtc gccttcttta cttttgttac tgatttgtaa aattattttg
    12841 cgtcagctaa accatcgagg tagcgttccg catcaagtgc tgccatgcag ccagtaccgg
    12901 ccgaagtaat ggcctggcga taaatgtgat ccatcacgtc gcctgcggca aagacgccag
    12961 gaatgctggt ctgggtggca ttaccatgaa tacccgactg tactttgatg tagccgtttt
    13021 ccagttccag ctgcccttcg aaaatcgcag tattcgggct gtgaccgata gcaacaaaca
    13081 gaccggcaac gtcgagtgac tcgatgttat cgctgttttg cgtatcgcgc agacgaacgc
    13141 cagtgacacc catttgatcg ccggtcactt cttccagcgt acggttggtg tgcagaatga
    13201 tgttgccgtt ctccacttta tccatcagac gcttaataag gattttttcc gcgcggaaac
    13261 cgtcacggcg gtgaatcaga tgcacttccg aagcgatgtt agacagatac agcgcctctt
    13321 caaccgcggt attgccgccg ccgatgaccg caactttctg gttgcgatag aagaaaccgt
    13381 cgcaggttgc acaagcagaa accccacggc ctttaaaggc ttcttcagag ggcaggccga
    13441 gatagcgtgc agaagctccg gtggcaataa tcagcgcgtc gcaagtgtat tcgccgttat
    13501 cgccattcag acggaacgga cggttttgca gatccacctt gttgatatga tcaaaaatga
    13561 tctcagtttc aaacttggtg gcatgttcgt gcatgcgctc cattaataac ggaccggtca
    13621 gatcgtttgg atcaccaggc cagttttcca cttccgtggt ggtggtcagt tggccgcctt
    13681 tttccatgcc ggtaatcagc acaggttgca ggttggcgcg cgccgcgtag acagcagcgg
    13741 tgtatcccgc cgggcctgaa cccaggataa gcagtttact gtgtttggtc gtgcccatga
    13801 gatccccata gttgttggca gacaatgggc aggattgtag ggaatttaca gacgtaaaaa
    13861 aagagtatga cgattttgtt aacaatttgt gcaatcggca gcatcgataa gcaggtcaaa
    13921 ttctcccgtc attatcacct ctgctactta aatttcccgc tttataagcc gattaaatga
    13981 tgaataaacg cccctgttaa tgaatatctg gcatgttgta ctaaaaatcg atgttttgct
    14041 ttgacaatcc cctggtgttt tgcgaaaaca ttcgaggaag aaaaaaaaca gtattcttat
    14101 atgcgcataa ccatgcatgt aaataccatg tttaccgtgc tagtgaaatc tacgtatggc
    14161 gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct tctcaccgta
    14221 gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac gggtagcgac
    14281 tctgaacagt gatgtttcag gggcagacag gagtagggaa ggaatacaga gagacaataa
    14341 taatggtaga tagcaagaag cgccttggca aagatctcga ccgtatcgat cgtaacattc
    14401 ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa cgtgtgggac
    14461 tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg tttattcagg
    14521 gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta ttcgttgaga
    14581 ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct gtacaaaaac
    14641 ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg ttgaaaacac
    14701 gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg cgtctgcctg
    14761 gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt aatcgtctgg
    14821 ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat tacactcctg
    14881 ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt tagcatcggg
    14941 caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg aagacaaaga
    15001 agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcgttgc tgatccttat
    15061 tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt cggaccccag
    15121 ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc ccggtgcgtg
    15181 gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc ccgtcattat
    15241 tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca ttgattattt
    15301 tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct gtggtctggc
    15361 ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg gcagcttact
    15421 aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc tgctctgcgt
    15481 ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg ctgaaaaact
    15541 cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc gcgatgatac
    15601 ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa atcacggcaa
    15661 acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc gtaaacggtt
    15721 ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt tctccggtaa
    15781 gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg ccgaccctga
    15841 cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat acgatccatt
    15901 attaaacggt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta ccacggcggc
    15961 acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg cctctgttga
    16021 tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac aaacgggaga
    16081 gccggttatt gctcctgcac cggaaggtta cccacagcag ccacaatatg cgcagcctgc
    16141 agtgcaatat aatgagccgc tgcaacaacc agtacagcca cagcagccgt attatgcacc
    16201 tgcagctgaa caacctgtgc aacaacctta ctacgctcct gcggcggaac aacctgttca
    16261 gcaaccgtat tacgcccctg cgccagaaca accggtggca ggtaacgcct ggcaagccga
    16321 agagcagcaa tccacttttg ctccacagtc tacataccag actgagcaaa cttatcagca
    16381 accagccgct caggagccgt tgtaccaaca gccgcaaccc gttgaacagc agcctgttgt
    16441 ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt ccgccacttt actattttga
    16501 agaagtggaa gagaagcgag cccgtgaacg tgaacaactt gcggcctggt atcaaccgat
    16561 tccagaaccg gttaaagaac cagaaccgat caaatcttcg ctgaaagcac cttctgttgc
    16621 agcagtacct ccagtagaaa ccgctgccgc tgtttctccg ctggcatctg gcgtgaaaaa
    16681 agcgacactg gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca gtctggcaaa
    16741 tagcggtgga ccgcgtccgc aggtcaaaga ggggattggc ccgcagttgc cacgaccgaa
    16801 acgtatccgc gtgccaactc gccgtgaatt ggcgtcttac ggtattaagc tgccctcaca
    16861 gcgtgcggcg gaagaaaaag cccgtgaagc ccagcgcaat cagtacgatt ctggcgatca
    16921 gtacaacgat gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc agttcgccca
    16981 gacacagcag caacgctatg gcgaacagta tcaacatgat gtgcccgtaa acgcagaaga
    17041 tgcagatgct gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc aacaacaacg
    17101 ttattccggc gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt ttgaattttc
    17161 gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg ttgtttacgc caattgttga
    17221 acctgtacag cagccgcaac aaccggttgc accgcagcag cagtatcagc agccacaaca
    17281 gccggttgcg ccacagcagc aatatcagca gccgcaacag ccggttgcgc cgcagccaca
    17341 atatcagcag ccgcaacaac cggttgcgcc acagcagcaa tatcagcagc cgcaacaacc
    17401 agttgcgccg cagccgcagt atcaacagcc gcaacaaccg gttgcgccgc agcagcagta
    17461 tcagcagcca caacagccag ttgcgccaca accgcaggat accctgcttc atccgctgtt
    17521 gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg acgccgctgc cttctctgga
    17581 tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc ttgaacaaat
    17641 ggctcgcctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg ttgtcaatta
    17701 ctctccgggg ccggttatca ctcgctttga attgaacctg gcaccgggcg taaaagcggc
    17761 gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg cggtgcgtgt
    17821 cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccgaata aaaaacgaca
    17881 aaccgtttat ctgcgcgaag ttttggataa cgccaaattc cgcgataatc cgtcgccatt
    17941 aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc tggcgaaaat
    18001 gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa tctgtcggtg tgaacgcgat
    18061 gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca tgatcgaccc
    18121 gaaaatgctg gagctttcgg tttatgaagg cattccgcat ctgttaacgg aagtcgttac
    18181 tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg agcgtcgtta
    18241 taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa aaattgctga
    18301 agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg acagtatgga
    18361 tgcccagcat ccggtgctga aaaaagaacc atacattgtg gtgttggttg acgaatttgc
    18421 cgacctgatg atgacggtag gtaaaaaagt ggaagagctg atagcacgtc tggcgcaaaa
    18481 agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg ttgatgttat
    18541 tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat ccagtaagat
    18601 tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggga tgggggatat
    18661 gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt ttgttcgcga
    18721 tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac agtatgttga
    18781 tggcatcact tccgacagtg aaagcgaagg tggcgcgggc ggtttcgatg gcgctgaaga
    18841 actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca aagcgtcaat
    18901 ttctggcgta cagcgtcagt tccgcattgg ttataaccgt gcagcgcgta ttatcgaaca
    18961 gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg aagtgctggc
    19021 tccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg tcgcatccgg
    19081 cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta ttagcctgga
    19141 atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata atgatgaaaa
    19201 aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt tgggctgatg
    19261 ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc agcttcacac
    19321 aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga
    19381 aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt ctggtttctg
    19441 acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca acttggctga
    19501 aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc agcgactggc
    19561 agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa gccagcaatg
    19621 gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat cagtttagcg
    19681 cggtggagca ggacgatcag cgcagcagtt atcaactgaa atcccagcaa aatggggctg
    19741 tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta
    19801 agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac ttttcaacct
    19861 ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggccagca acatttgctg
    19921 gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atctgcattc tatgatcctc
    19981 tgggggccac cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg ctatgcgaac
    20041 gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga aagagattcg cgaggcgatc
    20101 gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt tgacgaagtg
    20161 caccgattca acaaaagcca gcaggatgca tttctgccac atattgaaga cggcaccatc
    20221 acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc actgctttcc
    20281 cgtgcccgtg tctatctgtt gaaatccctg ggtacagagg atattgagca agtactaact
    20341 caggcgatgg aagacaagac ccgtggttat ggtggtcagg atattgttct gccagatgaa
    20401 acacgacgcg ccattgctga actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg
    20461 gaaatgatgg cggatatggc cgaagtcaat gatagcggta agcgggtcct gaagcctgaa
    20521 ttattgaccg aaatcgccgg tgagcgaagc gcccgctttg ataacaaagg cgatcgcttt
    20581 tacgatctca tttccgcact gcataagtcg gtacgtggta gcgcacccga tgcggcgctc
    20641 tactggtatg cgcgaattat taccgctggt ggcgatccgt tatatgtcgc gcgtcgttgc
    20701 ctggcaattg cttccgaaga tgtcggcaat gccgacccac gggcgatgca ggtggcaatt
    20761 gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag
    20821 gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc gtttaaagct
    20881 gcgctggcgg atgctcgcga acgcccggat tatgacgtgc cggttcattt gcgtaatgcg
    20941 ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc tcatgatgaa
    21001 gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca aacacgctat
    21061 tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc ctggctggct
    21121 gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg cggtaatgtt
    21181 gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt cgataagcac
    21241 aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa
    21301 ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga agagcgtcgt
    21361 aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg atcgaaatcc
    21421 attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga agtgaacaaa
    21481 ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc tgaaattcgc
    21541 gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg taaagacgaa
    21601 aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt tgaagttcgt
    21661 gaccacgtga cgctgggtga aatgcactct ggcctcgact ttgcagctgc agttaagctg
    21721 actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg cgcactgtcg
    21781 cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta tgttccgtac
    21841 ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc tggcgatctg
    21901 ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct gatcccaacg
    21961 gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga tgatctgcca
    22021 attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata tggtcgtgac
    22081 acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc
    22141 ccggaagact caatggcggc gctggaagag atgactggtc atgcagaaaa agtcctgcag
    22201 ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg ctttggcgct
    22261 tgcaaaactt acgacctgga agtatggatc ccggcacaga acacctaccg tgagatctct
    22321 tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg ccgcagcaag
    22381 tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc tgttggtcgt
    22441 acgctggttg cagtaatgga aaactatcag caggctgatg gtcgtattga agtaccagaa
    22501 gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat ttttctgaat
    22561 ctaaaaagcg cctgcgggcg ctttttttgt ctccctttga taccgaacaa taattactct
    22621 tcacttacac gtaatactac tttcgagtga aaatctacct atctctttga ttttcaaatt
    22681 attcgatgta tacaagccta tatagcgaac tgctatagaa ataattacac aatacggttt
    22741 gttactggaa tcaatcgtga gcaagcttga gtgagccatt atgaaaacga aaatccctga
    22801 tgcggtattg gctgctgagg tgagtcgccg tggtttggta aaaacgacag cgatcggcgg
    22861 cctggcaatg gccagcagcg cattaacatt accttttagt cggattgcgc acgctgtcga
    22921 tagcgccatt ccaacaaaat cagacgaaaa ggttatctgg agcgcctgta cagttaactg
    22981 tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat atgtcgaaac
    23041 ggacaatacc ggcgatgaca attacgacgg cctgcaccag gttcgcgcct gcctgcgtgg
    23101 gcgttccatg cgtcgccgtg tctacaatcc ggaccgcctg aaatatccga tgaaacgagt
    23161 cggggcgcgc ggtgaaggca aattcgagcg cattagctgg gaagaagcct acgacatcat
    23221 cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac gagtctatct atctgaacta
    23281 tggcaccggt acgctgggcg gcaccatgac ccgctcctgg ccgccgggaa ataccctggt
    23341 cgcgcggctg atgaactgct gcggcggcta tctgaaccat tacggcgact actcctccgc
    23401 gcaaattgcg gaaggtttga actataccta cggcggctgg gcagatggca acagcccgtc
    23461 ggatatcgaa aacagtaagc tggtagtgct gtttggtaat aaccctggcg aaacgcgaat
    23521 gagtggcggt ggggtgactt actatcttga acaggcacgc cagaaatcta atgcccgcat
    23581 gatcatcatc gatccgcgct ataccgacac cggtgccggg cgcgaagatg agtggatccc
    23641 tattcgtccg ggaacagatg ccgcactggt taacggtctg gcgtacgtca tgatcactga
    23701 aaacctggtg gatcaggcat tcctcgataa atattgcgtt ggctacgatg agaaaaccct
    23761 gccagccagt gcgccgaaaa atggccacta taaagcttat attctgggtg aagggccaga
    23821 tggcgtggct aaaacaccgg aatgggcctc acaaatcacc ggtgttccgg cagacaaaat
    23881 catcaaactg gctcgtgaaa tcggcagtac caaaccggcg tttatcagcc agggatgggg
    23941 cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct atctcgatgc tggcgattct
    24001 gaccggtaac gttggtatta atggagggaa cagcggcgcg cgtgaaggtt catacagctt
    24061 accgtttgtc cgtatgccga ccttggaaaa cccgatccag accagcattt cgatgtttat
    24121 gtggaccgat gccattgaac gtggcccgga aatgacggcg ctgcgtgatg gtgtacgcgg
    24181 gaaagataag ctggatgtgc cgatcaaaat gatctggaac tatgccggta actgcctggt
    24241 taaccagcat tctgaaatca accgtaccca tgaaatcctt caggatgata agaagtgcga
    24301 gctgattgtg gttatcgact gccacatgac ctcatcggcg aaatatgctg acatcctgct
    24361 gcctgactgc accgcttccg aacagatgga ctttgcgctg gatgcatcct gcgggaatat
    24421 gtcttacgtg attttcaacg atcaggtgat taaaccgcgc tttgaatgta agaccatcta
    24481 tgaaatgacc agcgaactgg caaaacgtct tggcgttgag caacagttta ctgaaggccg
    24541 tacccaggaa gagtggatgc ggcatctgta tgcccagtcg cgggaagcga ttcctgaact
    24601 gccaacgttt gaagagttcc gcaagcaggg gatctttaaa aagcgcgacc cacaagggca
    24661 tcacgttgct tataaagcct tccgtgaaga tccgcaggca aacccactga ctacgccatc
    24721 gggcaaaatt gagatttatt cgcaggcgct ggctgacatt gccgctacct gggaattgcc
    24781 agaaggcgat gtgatcgatc cactgccgat ctacacgccg ggctttgaaa gttatcagga
    24841 tccgctgaac aaacagtatc cgctgcagct tacgggtttc cactataaat ctcgcgttca
    24901 ctcaacttac ggcaacgttg atgtgctgaa agcagcttgc cgtcaggaaa tgtggatcaa
    24961 cccgcttgat gcgcaaaaac gcggtatcaa caacggcgat aaagtcagga tctttaacga
    25021 tcgtggtgaa gttcatattg aggcgaaagt gacgccacga atgatgccag gtgtggtcgc
    25081 actgggtgaa ggtgcctggt atgacccgga tgcaaaacgt gtcgataagg gtggttgtat
    25141 taacgtactg accactcaac gtccgtctcc tctcgctaag gggaatccgt cacatacaaa
    25201 ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac aacccagtat ggatttttta
    25261 ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct ggcttgtaaa gactacaaag
    25321 atttgacgcc agaagtcagc ttccgccgca tttatgaata tgctggcggc gactggcagg
    25381 aagataacgg tgtctggcac cagaacgtgt ttgcctacta tctgtcgatt tcgtgcaacc
    25441 actgcgaaga tccggcttgt actaaagtct gcccgagcgg tgcgatgcat aaacgtgaag
    25501 atggttttgt tgtggtcgat gaagatgtgt gcattggctg ccgctactgc catatggctt
    25561 gcccgtatgg cgcaccgcaa tataacgaaa cgaaaggcca tatgaccaaa tgcgatggtt
    25621 gttatgaccg tgttgcggag ggtaaaaagc cgatctgtgt tgaatcttgt ccgctgcggg
    25681 cgctggattt cggccctatc gacgagctgc gtaaaaaaca tggcgatctg gcggccgttg
    25741 cgccgttgcc gcgagctcac tttaccaaac cgaatattgt gatcaaaccc aatgccaata
    25801 gccgcccgac cggggatacc actggctatc tggcaaaccc gaaggaggtg tgagatggga
    25861 agtggatggc atgaatggcc gctgatgatc ttcacggtct tcgggcaatg tgtggcgggt
    25921 ggttttatcg ttctggcttt ggcgctgctc aaaggcgacc tgcgagcaga agcccagcag
    25981 cgtgttatcg cctgcatgtt tggcttatgg gtgctgatgg gcattggctt tatcgcttct
    26041 atgctccatc ttggttcacc gatgcgcgct tttaactcgc tcaaccgggt aggggcttca
    26101 gcactcagta acgaaatcgc cagcggttcg atcttttttg ccgtaggcgg catcggctgg
    26161 ttgctggcaa tgctgaaaaa gctgtcaccg gcattgcgta cgctttggct ggtggtgacg
    26221 atggttcttg gcgtcgtctt tgtctggatg atggtgcgtg tgtataacag cattgatacc
    26281 gttccgacct ggtacagcat ctggacgccg atgggcttct tcctgacgat gttcatgggc
    26341 ggcccgttac tggggtacct gttgctgagt ctggctggcg tcgatagctg ggcgatgcgt
    26401 ctgctgccag ccatttccgt actggcactg gtggtgagtg gcgtggtgtc agtgatgcag
    26461 ggcgcagagc tggcgactat tcatagttct gtgcagcagg ccgcagcgct ggtgccggac
    26521 tatggtgcgc tgatgtcctg gcggatcgtg cttttggccg ttgccctgtg cttgtggatt
    26581 gcaccacagc taaaaggtta tcagcctgcg gttccgctac tatccgtctc attcattctg
    26641 cttctggcag gggaattaat cggtcgcggc gtattctatg gtttgcatat gaccgtgggg
    26701 atggccgtcg caagctaatc agtaacaacc ggggtttcgg ccccggttct cttttatttc
    26761 tgcttcgtta acgtgtcata actggtcatc aaattacgat aatcaggaat atggttagag
    26821 aacaacgtcg ccagtccttc aatatcatta cgccagtcac ggtgcagctc gcaagccacg
    26881 ccgaaccatg tcatcaattg cgcacctgct tgcgacatgc gatcccatgc cgaatgccgg
    26941 gtaatttcat taaaggtccc tgacgcgtca gtcacaacaa acacatcaaa cccttcttca
    27001 atggctgaaa gcgccgggaa tgccacgcaa acttcggtta ccacaccggc aataattaac
    27061 tgttttttac ctgtcgcttt gactgctttt acaaaatctt cgttatccca ggcgttaata
    27121 ttgcccgggc gagcaatgta aggtgcatcg gggaattgtg ctttcagttc tggaactaat
    27181 gggccgttag gaccggtttc aaaactggtg gtaagaatag ttggtaagtt gaaatactta
    27241 gccaggtcac ccagcgccag cacgttattt ttaaacttat cgggttcgat atcccgtaca
    27301 agggaaagta aaccagcctg gtgatcaaca agcaaaacag cagcatcatt tttatcaaga
    27361 cgaacatacg gtttggtcat cctcttctcc tttcgagagt cagtggtacg cctgaaatcg
    27421 gtcagacgtg atgcattgct ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat
    27481 gaaatttttc gactgaacga agcgtatgaa atttgtgtta gatcaataaa aacaatcaga
    27541 tgagattaat catctcttca gtattagcgt tatgaatgag taatataaat cgataatacc
    27601 gccgttgcga tatgcgcgta ttttcaccca ttgacaatgt ttttggcggt ggcatgatgc
    27661 gcatgaaatt tgaacttcct cacggtttta attcatgtcc acgtataccc ggcctgtcat
    27721 gcttttgctg tctggcctgc ttttgttgac tctggcgatc gcggtgttaa atacactcgt
    27781 gccgctttgg ctcgcccagg aacacatgtc cacatggcag gtaggcgttg tcagctcatc
    27841 ctattttacc ggcaaccttg tcggtacatt gctgacaggg tatgtcatta aacgcattgg
    27901 ttttaaccgc agctattatc tggcctcctt catttttgcc gctggctgtg ccggccttgg
    27961 cctgatgatt ggattctgga gttggttggc gtggcgtttt gtcgcgggcg tcggctgtgc
    28021 catgatttgg gtagtcgttg agagcgcgct gatgtgcagt gggacgtcac gtaaccgtgg
    28081 gcgtttgctt gctgcataca tgatggttta ttacgtggga acgtttttag gccagttact
    28141 ggtcagcaag gtctcaaccg agctgatgag cgtattgccg tgggttacag gtttgacatt
    28201 ggcagggatc ttaccgctgt tgtttacgcg tgtcctgaat cagcaggctg aaaaccatga
    28261 ttcgacgtca attacggcaa tgctaaaact ccgtcaggcg cggcttggcg tgaatggctg
    28321 cattatctca ggaatcgttc tgggatctct atatggcctg atgcctctgt acctcaatca
    28381 caaaggggtg agcaatgcca gtattggttt ctggatggcg gtactggtca gtgcgggtat
    28441 cctcggacaa tggccgattg gacgtctggc ggataagttt ggtcgactgc tggttttgcg
    28501 tgttcaggtc tttgtcgtca ttctcggcag tattgcgatg cttagccagg cggcgatggc
    28561 cccagcgtta ttcatcctcg gtgccgctgg ctttacgcta tatccggtgg cgatggcatg
    28621 ggcttgcgag aaagttgaac atcatcaact ggtggcgatg aaccaagcct tactgttgag
    28681 ctatactgtg ggaagtctgc ttggcccgtc atttaccgct atgctaatgc agaatttctc
    28741 cgataattta ttgtttatca tgatcgccag cgtatcgttt atctatttgc tgatgctgct
    28801 gcgcaacgcc ggtcatacgc cgaaacccgt tgctcacgtg taaatgaatt caagcagagt
    28861 gtgaacttac tgtttcacac tctgcttttt tgtttcttct atctgacttg ctttattcca
    28921 aattttattc gtttaaaaat aaaatgtgta gcaggttata attttgcatt tcgctatttc
    28981 cgcacttctt atttgccgcg cataatccct cgttttacag atgcccctat aattttggcg
    29041 aaggatttgt ctatggctgg gaatgttcag gaaaaacagc tgcgatggta caacattgcg
    29101 ctgatgtctt ttatcactgt ctggggtttt ggcaacgttg ttaataacta cgccaaccag
    29161 gggctggtgg ttgttttttc atgggtgttt atctttgcgc tctatttcac accttatgcg
    29221 ctaattgttg gtcagttagg ctcgaccttc aaagatggga agggcggggt cagtacctgg
    29281 attaaacaca cgatgggacc cggactggct tatctcgccg cgtggaccta ctgggtggtg
    29341 catattccct atctggcaca aaaaccctag gcaattctga ttgcgctcgg ttgggcgatg
    29401 aaaggcgacg gttcgctaat caaagaatat tcagtcgtag cgttacaggg gttaacgctg
    29461 gtgctgttta tcttctttat gtgggttgct tcacgcggta tgaaatcgct gaaaatcgtc
    29521 ggttctgtgg cagggattgc tatgtttgtt atgtcgctcc tgtatgtggc gatggcggta
    29581 accgcgcctg caattactga agtgcatatt gcgaccacaa acattacctg ggaaacgttc
    29641 attcctcata tcgactttac ctacattacc actatttcaa tgctggtttt cgcggttggc
    29701 ggagcagaga agatttctcc ttacgttaat caaacgcgca acccaggaaa agaatttcca
    29761 aaagggatgt tatgcctggc ggtgatggtt gcggtttgtg ccattctggg ctcgctggcg
    29821 atggggatga tgtttgattc gcgtaatatc ccggatgact taatgaccaa cggtcagtat
    29881 tacgcctttc agaagctggg cgagtattac aacatgggta atactttaat ggtgatttac
    29941 gccattgcga ataccctggg acaagtagcg gcgctggtat tctcgattga tgccccgctt
    30001 aaagtgctat taggtgatgc tgacagcaaa tatattccag ccagtttatg tcgtaccaac
    30061 gcttctggta cgcccgttaa tggctatttt ctgaccctgg tactggtggc gattctgatt
    30121 atgctgccga ctctcggcat tggtgatatg aacaatctct ataaatggct gttgaacctt
    30181 aattcggtag tgatgccgct gcgttattta tgggtatttg ttgcatttat tgcagtcgtt
    30241 cgcctggcgc agaaatataa accagaatat gtctttattc gtaataagcc tctggcaatg
    30301 accgtcggga tttggtgttt tgcctttacc gcctttgcct gtttgacggg gatcttcccg
    30361 aaaatggaag ccttcactgc agagtggacc ttccagttgg cgctgaatgt tgcaacgccg
    30421 tttgtgctgg taggattagg actgatattc ccgctgctgg cgcgtaaagc gaatagtaaa
    30481 taattattgt ggtcggtgac gctgactctg gcagatctga cccgttttta cataatcgag
    30541 aaatgcgcgc aagccgcagg acatatactg gcggtttggg taatagatct ggaagcctgg
    30601 acgctctgtg atccagtctt ccagtacaca ttctaaacga ccggtatcaa ggtactcctt
    30661 gatctgttca tagagcagat acccgatacc agcacccatc aaaacggcat ccagttcagc
    30721 atcaacatca t
//
LOCUS       DAJTVJ010000042        30004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000042 DAJTVJ010000000
VERSION     DAJTVJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.042"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            40..582
                     /locus_tag="OO448_001832"
     CDS             40..582
                     /locus_tag="OO448_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6337050.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            665..1366
                     /locus_tag="OO448_001833"
     CDS             665..1366
                     /locus_tag="OO448_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6337051.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1391..3990
                     /locus_tag="OO448_001834"
                     /pseudo
     CDS             1391..3990
                     /locus_tag="OO448_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3981..5051
                     /locus_tag="OO448_001835"
     CDS             3981..5051
                     /locus_tag="OO448_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6337052.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5063..5605
                     /locus_tag="OO448_001836"
     CDS             5063..5605
                     /locus_tag="OO448_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6337053.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5613..6128
                     /locus_tag="OO448_001837"
     CDS             5613..6128
                     /locus_tag="OO448_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6337054.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6094..6831
                     /locus_tag="OO448_001838"
     CDS             6094..6831
                     /locus_tag="OO448_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6337055.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6942..7952
                     /gene="pyrD"
                     /locus_tag="OO448_001839"
     CDS             6942..7952
                     /gene="pyrD"
                     /locus_tag="OO448_001839"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6337056.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8126..8668
                     /gene="zapC"
                     /locus_tag="OO448_001840"
     CDS             8126..8668
                     /gene="zapC"
                     /locus_tag="OO448_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6337057.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8665..9774)
                     /gene="ycbX"
                     /locus_tag="OO448_001841"
     CDS             complement(8665..9774)
                     /gene="ycbX"
                     /locus_tag="OO448_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6337058.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10018..12126
                     /gene="rlmKL"
                     /locus_tag="OO448_001842"
     CDS             10018..12126
                     /gene="rlmKL"
                     /locus_tag="OO448_001842"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6337059.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12138..14045
                     /locus_tag="OO448_001843"
     CDS             12138..14045
                     /locus_tag="OO448_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6337060.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14175..15428
                     /gene="pqiA"
                     /locus_tag="OO448_001844"
     CDS             14175..15428
                     /gene="pqiA"
                     /locus_tag="OO448_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6337061.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKATCPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15433..17073
                     /gene="pqiB"
                     /locus_tag="OO448_001845"
     CDS             15433..17073
                     /gene="pqiB"
                     /locus_tag="OO448_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6337062.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17070..17633
                     /gene="pqiC"
                     /locus_tag="OO448_001846"
     CDS             17070..17633
                     /gene="pqiC"
                     /locus_tag="OO448_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6337063.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17889..18056
                     /gene="rmf"
                     /locus_tag="OO448_001847"
     CDS             17889..18056
                     /gene="rmf"
                     /locus_tag="OO448_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6337064.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18126..18644)
                     /gene="fabA"
                     /locus_tag="OO448_001848"
     CDS             complement(18126..18644)
                     /gene="fabA"
                     /locus_tag="OO448_001848"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6337065.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18713..20473)
                     /locus_tag="OO448_001849"
     CDS             complement(18713..20473)
                     /locus_tag="OO448_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6337066.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20659..21111
                     /gene="matP"
                     /locus_tag="OO448_001850"
     CDS             20659..21111
                     /gene="matP"
                     /locus_tag="OO448_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6337067.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21187..22227)
                     /gene="ompA"
                     /locus_tag="OO448_001851"
     CDS             complement(21187..22227)
                     /gene="ompA"
                     /locus_tag="OO448_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6337068.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22582..23091)
                     /gene="sulA"
                     /locus_tag="OO448_001852"
     CDS             complement(22582..23091)
                     /gene="sulA"
                     /locus_tag="OO448_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6337069.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23310..23939
                     /gene="sxy"
                     /locus_tag="OO448_001853"
     CDS             23310..23939
                     /gene="sxy"
                     /locus_tag="OO448_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6337070.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23902..26064)
                     /gene="yccS"
                     /locus_tag="OO448_001854"
     CDS             complement(23902..26064)
                     /gene="yccS"
                     /locus_tag="OO448_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6337071.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26074..26520)
                     /locus_tag="OO448_001855"
     CDS             complement(26074..26520)
                     /locus_tag="OO448_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6337072.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26643..28697
                     /gene="helD"
                     /locus_tag="OO448_001856"
     CDS             26643..28697
                     /gene="helD"
                     /locus_tag="OO448_001856"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6337073.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28729..29187)
                     /gene="mgsA"
                     /locus_tag="OO448_001857"
     CDS             complement(28729..29187)
                     /gene="mgsA"
                     /locus_tag="OO448_001857"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6337074.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29283..29945)
                     /locus_tag="OO448_001858"
     CDS             complement(29283..29945)
                     /locus_tag="OO448_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6337075.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7734 a         7085 c         7880 g         7305 t
ORIGIN      
        1 aggcattatc atatttacga agagatcagg atgatgacga tgaaaaaaag tgtattgacg
       61 gcgtttataa ctgtggtatg tgcaacgtcc agcgttatgg cagctgatga taatgctatc
      121 acggatggca aagtaacatt taatggtaaa gttattgctc cagcttgtac cctggtagct
      181 gcgacgaaag attcagtagt cacattgcca aatgtcagcg ccaccaagtt gcaaactaat
      241 ggagcagtct ctggcgttaa aactgatgta ccaattgcct tagaaggctg tgatgttact
      301 gtaactaaaa atgcgacgtt tacctttagt ggtacggctg acggcgtgca acctacagca
      361 tttgccaacc aggctaccac cgatgcagcg acgaatgttg ctctacaaat gtatctgcca
      421 gatggttcta catcggtaac tcccggtacc gaaacgtcga atattcagtt agcggattct
      481 gctgagcaaa cagtgacctt taaagttgat tatatcgcta cggggaaagc gacttctggt
      541 aatgtgaatg cggtaacaaa tttccatatt aactattatt aatagaactc attaattgtt
      601 ttattaatta gtacccctct agtgttctgg aggggatatt catatttttt aagagtgact
      661 atttatgaaa acttgcataa caaagggaat tgtgaccgta agtttaacgg caatattact
      721 cagctgctca tccgcatggg cggcaggtaa aggcgggatt ggacttgcag caacacgtct
      781 tgtttattca gaaggtgaag agcaaatttc actgggtgta cgtaacacca gtccggatgt
      841 tccttatctt attcagtcat gggtgatgac cccagataat aaaaaatcag cagattttat
      901 tattacacca ccgctatttg tgctgaatcc ggctaatgag aatctgttac gcattatgta
      961 cattggtgcg ccgttggcga aagacagaga aacccttttc ttcactagcg tacgggcagt
     1021 cccttcaaca acgaagcggg aagagggaaa taccctgaag attgccacac aaagcgtcat
     1081 caaacttttc tggcgaccaa aaggtttagc gtatccctta ggcgaggctc cggcgaaact
     1141 gcgttgcact tcgtcagctg acatggttac ggtcagtaac ccaacacctt atttcattac
     1201 cctgacagac ctgaaaatag ggggaaaggt agttaaaaat caaatgattt ccccctttga
     1261 taaataccaa ttctctctgc caaagggggc caaaaatagc agcgtaacgt atcgaaccat
     1321 caatgattac ggggcggaaa cgccacaact caactgtaaa tcgtaagccg tcttcagtta
     1381 agagagcgag atgtatagaa ctcatcgaca acacagcctg ttaagctctg gtggagtgcc
     1441 atcgtttatt ggtgggctgg tggtgtttgt gtcggcagcg ttcaatgcac aagctgaaac
     1501 ctggttcgat cctgcttttt tcaaagatga tccctcaatg gtggccgatt tgtctcgttt
     1561 cgaaaaagga caaaaaataa cgccaggggt ttatcgtgtc gatattgttc tgaatcagac
     1621 aattgtagat acgcgcaacg tcaattttgt tgagataacg ccagagaagg ggattgccgc
     1681 ctgtttgacg actgaaagcc tggatgcaat gggtgttaat actgatgcgt ttccggcttt
     1741 taaacaactg gacaaacaag cgtgtgtgcc attggcggag attattccgg atgccagcgt
     1801 aacgtttaat gtgaataaac tccgtctgga aatttcagta ccgcaaatcg ccatcaaaag
     1861 taatgcccgt ggttatgtcc cccctgaacg ttgggatgaa gggatcaacg cgctattact
     1921 gggatattcg tttagcgggg ctaacagtat tcatagcagc gcagacagtg attctggcga
     1981 cagctatttt ctgaatttaa acagtggcgt taatttaggc ccatggagat tgcgcaacaa
     2041 ttcaacatgg agtcgcagta gtggccaaac cgcagaatgg aagaatctca gcagctattt
     2101 gcagcgggcg gttattccac taaaaggcga actgaccgta ggtgatgatt atactgcagg
     2161 cgattttttc gatagtgtca gctttcgtgg tgtgcagctg gcgtcagatg acaacatgct
     2221 gccagacagc ctgaaagggt ttgcgcctgt ggtgcgtggt atcgccaaaa gcaatgccca
     2281 gataacgatt aagcaaaatg gttacaccat ttaccaaact tatgtatcgc ctggtgcttt
     2341 tgaaattagt gatctctatt ccacgtcgtc gagcggtgat ttgttagttg aaatcaaaga
     2401 agcggacggc agcgtcaata gctacagcgt accgttttcc agcgtgccat tactccagcg
     2461 tcaggggcga atcaaatacg cggtgacact ggcgaaatac agaaccaata gtaatgaaca
     2521 gcaggagagc aaatttgcct aggccacgtt gcagtggggc ggaccgtggg gaacgacatg
     2581 gtatggtggt ggacaatatg ctgaatatta ccgtgccgcc atgtttggtc tgggatttaa
     2641 ccttggcgat ttcggagcaa tttcgttcga tgcgacccag gcgaagagta cgctggcaga
     2701 ccaaagcgaa cataaaggtc agtcatatcg ttttctgtat gccaaaacgc tcaaccaatt
     2761 gggcactaat tttcaattga tgggctatcg ctattcgacg tcgggtttct acaccctttc
     2821 cgacaccatg tataaacata tggatggcta cgaatttaat gacggtgatg atgaagatac
     2881 gccgatgtgg tcgcgttatt acaatttgtt ttacaccaaa cgtggcaaac tgcaggtcaa
     2941 tatctcccag caattaggcg agtacggttc gttttattta agtggtagcc agcaaactta
     3001 ctggcatacc gatcaacagg atcggctatt acagtttggc tacaacacgc aaattaaaga
     3061 tctctcgctg gggggttcct ggaactacag taagtcccgt ggtcaacctg atgctgatca
     3121 ggtgtttgca ctaaattttt ccctgccgct caatctgttg ctccccagaa gtaatgatag
     3181 ctataccagg aaaaaaatta cgcctggatg acctctaaca ccagcatcga taacgaaggg
     3241 cacattacac aaaacctggg tttaacggag acactactcg atgacggtaa cctgagctac
     3301 agcgtgcaac agggatataa cagcgagggg aaaacggcta atggtagcgc cagcatggac
     3361 tacaaagggg cgtttgcaga tgcccgagtg ggctacaact acagcgataa cggcagtcaa
     3421 caacaactga actacgctct ttcaggcagt ttagttgccc attcacaggg cattaccctg
     3481 gggcaatcgc tgggggaaac taacgtgctg attgcagcac caggcgcaga gaatactcgt
     3541 gtggcgaaca gcaccgggct gaaaactgac tggcgcggat ataccgttgt cccctatgcc
     3601 acttcttatc gggaaaatcg aatcgcactt gatgcggcat cgttaaaacg taacgtggat
     3661 cttgaaaatg cagtagtcaa cgtggttccc accaaagggg cgttggttct ggcggagttc
     3721 aatgcccatg cgggagcaag ggtattaatg aaaacatcaa agcagggtat accgctgcgt
     3781 tttggcgcga tagcgacgct ggacggcgta cagactaata gcggcattat tgatgatgat
     3841 ggctcgctct atatgtccgg tttgcccgct cagggagcga taactgtacg ctggggcgaa
     3901 gctcccgatc aaatttgtca tatcagttac cagcttaccg aacaacaaat taactctgcg
     3961 attacgcgaa tggatgccat atgcagataa tctttggaga aaaatgcgtg ttattactac
     4021 gactattttt tgccgccgtc ttaatgctat ggtgcgctca aactgctgct tatagcgggc
     4081 agtgtcatac cactcagggg aatccgtata ttggcgtcaa ttttggcgtt aaaaccctgg
     4141 aggaagaaga aaatacggct ggagtagtta aagacaaatt ttatcagtgg aacgaatcga
     4201 atgattatta tgtttcctgt gattgcgata aagacaatgt cagaagtggc cgatgggcat
     4261 tcgccgcgga ttcaccgtta gtctatttag gcgacaactg gtacaaaatt aatgactatc
     4321 ttgccgccaa agttttattg caggttaaag gcagttctcc tactgcggtt cctttcgaaa
     4381 acgtgggcac aggggcagat acacgatggc atatttgcga tcccggcggt caacgtttag
     4441 gtggccaggg ggctagcggt aatagcggta gcttttccct gaaaatattg cagccgttcg
     4501 ttggttcggt cgtcattcct cctatggcgc tggcgcgatt atttgaatgc tacaacatac
     4561 ccgcaggtga ttcctgcacg actacaggta caccggtttt agtgtattac ctgtctggta
     4621 cgatcaattc acttggctca tgttccgtca atgccggaga aacaatcgag gtcgatctgg
     4681 gcgacgcatt tgcggctaac tttcgtgttg tagggcataa gcctcttggg gccagaacgg
     4741 cagaacttgc aattccagtc aggtgtaaca cgggaaacgc ggggttagtt aacgtcaacc
     4801 tgagtctgac ggcaaccaca gaccccagct atccccaggc gattaagacg tcacgtcctg
     4861 gcgtgggcgt ggtggtgacc gatagccaga acaacattat ttcccctgct ggtggaacat
     4921 taccgctctc tattcctgat gatgcagaca gtatcgcgcg aatgaatgtc tatccagtca
     4981 gcacgacagg tgtaccacca gaaaccgggc gatttgaagc cacggcaacg gtgagaataa
     5041 attttgatta acgcgtgaac gtatgaagaa agaaacgata tttcagtgcg ttatcttgtt
     5101 ctttagcatt cttaacatcc atgtcgggat ggctgggcct gaacaagtta gtatgcatat
     5161 ttatgggaat gtggtcgatc agggctgtga tgtcgccacc aaaagtgcat tacaaaatat
     5221 tcatattggt gattttaata tcagtgattt tcaggccgcg aataccgtaa gcactgctgc
     5281 tgatttgaat atcgatatca ccggttgtgc cgctagtatt actggcgcgg acgtcctttt
     5341 tagcggcgag gctgacaccc ttacgccgac actgctcaaa ctaactgaca caggcggaag
     5401 cggtggtatg gcaacgggga ttgccgtgca aattcttgat gcgcaaagtc agcaagaaat
     5461 cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca cactcaaata
     5521 tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga cggcggttct
     5581 ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc tggattctgt
     5641 tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg gtcaacatta
     5701 gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa atcaatgtgc
     5761 agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg gagaaatctt
     5821 tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgacgtc accttttcag
     5881 gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg gatgcagcca
     5941 gcgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt aaccaggcga
     6001 gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg caattgcgac
     6061 ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt gtacttcatt
     6121 atgactaata cgtggaacag attggcgctc ttgattttcg ccgttttatc gctgctggtg
     6181 gcgggtgaat tgcgggcagg agtggtggtc ggcggaacgc gatttatctt tccggcagac
     6241 agagaatcga tatctatttt actgactaat accagtcagg aatcctggct tattaacagt
     6301 aaaatcaacc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc agcaccatta
     6361 ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt gcgcttgctg
     6421 agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt aagcattgcc
     6481 agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg ctcggtattt
     6541 aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta tcaacaatta
     6601 cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta ctacattaac
     6661 ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagttgt gccgcctaaa
     6721 agccagcgtc agacaagctg gtgtcaggca atcgcaccgt gtcatgtcgc ctggcgggcg
     6781 ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg aaacaggttc
     6841 ggaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga actcgcacat
     6901 aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac cccttcgttc
     6961 gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt cagcaattac
     7021 gccgtattac aggaacgccg tttgaagcac tggtgcggca gaaagtgcct gcgaaacctg
     7081 ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt cttgataaag
     7141 acggggagtg cattgacgcg ttaggcgcga tgggatttgg atcgatcgag atcggtaccg
     7201 tcacgccacg tccacagcca ggtaatgaca agccgcgtct ctttcgtctg gtagatgccg
     7261 aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc gtagagaacg
     7321 taaaaaaggc ccattatgac ggcgtcctgg gtattaacat cggcaaaaat aaagatacgc
     7381 cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat gcctatgcgg
     7441 gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg ctgcaatatg
     7501 gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat ttgcaagcga
     7561 tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct gaagaagaat
     7621 tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt gcaaccaata
     7681 ccacactcga tcgttctctt gttcagggaa tgaaaaattg cgatcaaacc ggtggcttaa
     7741 gtggtcgtcc gcttcagtta aaaagcaccg aaattattcg ccgcttgtca ctggaattaa
     7801 acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct gcgcgtgaaa
     7861 agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt aaaggtccgc
     7921 cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct ctttataaga
     7981 taatcagggc tttattttca gccctggttg ttttatattc atcgctgttg cttatttaga
     8041 cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg tgttgctgcg
     8101 gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact ggcgttggta
     8161 ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc tatttcgctc
     8221 acgttttgcg cgcaagatgt tgactccaga tgccttttct cccgcaggct tttgcgttga
     8281 cgacgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt tatctaaaga
     8341 acaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgctatctca aaccgcaaat
     8401 gccgaaaagc tggcattttg tttcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc
     8461 ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg ttgaatctgg
     8521 cgaaaatgcc gcactatgcc tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat
     8581 gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg ttaatgttga
     8641 cagcttcagc ctcgaacagg cagtctaacg cgccaactta agtgcagtct tcggtacaca
     8701 gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg gcatgacttc
     8761 gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag aataagggat
     8821 acgaattccc tgattttcta attgctccag caacacctgt tggttatttc cacgaaatgc
     8881 ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt tggcggtatc
     8941 atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt ccacctcatc
     9001 gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat cgacatcgcc
     9061 gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg ccggatgttt
     9121 ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt taaccacatc
     9181 aaacaccaca tcaccaatgc gaatcacttt ccagcgatct tcttcccagg ctgacgcgcc
     9241 ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac aacgttgttg
     9301 gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag caaatgacag
     9361 aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg
     9421 cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg caattcgcgc
     9481 ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg
     9541 aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca caggtgaagg
     9601 agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt caggttccgt
     9661 gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat gtgtaagacc
     9721 aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg
     9781 gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta gctataatgc
     9841 gcaacaattt tcttagcgcc tgtcccatta ggctatttta tttgtcagtt tggccctggg
     9901 ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct gtccatgtcc
     9961 agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag tgacgatatg
    10021 aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa
    10081 aacctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa gggcgacaca
    10141 cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat gttgccgctg
    10201 ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc gatcaactgg
    10261 acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt gaatgacacc
    10321 atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg
    10381 cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc
    10441 tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg tttacatctg
    10501 cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc agccgctatt
    10561 gtgatgcgaa ccggctggca gccaggaaca ccgctgctcg atccgatgtg tggttccggt
    10621 acgttgctga ttgaagcggc gatgctagcg accgatcgcg caccaggctt gcaccgtggg
    10681 cgttggggct ttagcggctg ggtgcagcat gatgaagcta tctggcagga agtgaaagcg
    10741 gaagcgcaaa ctcgtgcccg taaaggcctg gctgagtata gctctcattt ttacggttcg
    10801 gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt
    10861 gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg
    10921 ccgtacggca cagtgttgag caacccgcca tacggggaac gtctggacag cgaaccggcg
    10981 ctgattgcgc tgcatagcct gctgggtcgg atcatgaaaa accagtttgg tggctggaat
    11041 ctctctttgt ttagtgcctc gccggatctg ctaagctgct tgcagctgcg tgcagacaaa
    11101 cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca cgttgccgaa
    11161 agcactccag acagcaaacc ggcgatggta gcggaagact acgccaaccg cctgcgtaag
    11221 aacctcaaaa aattcgagaa gtgggctcgc caggaaggaa ttgaatgtta ccgcctgtat
    11281 gacgccgatc taccggaata taacgttgcc gttgaccgtt atgccgactg ggtggtggtg
    11341 caggagtatg cgccgccaaa aactattgat gctcacaaag cgcgtcagcg tctgttcgat
    11401 attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt gctgaaaacc
    11461 cgtgaacgcc agaagggcaa aaaccagtac cagaaactgg gcgagaaggg cgagtttctt
    11521 gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct cgatactggt
    11581 ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa aggcaaagat
    11641 ttcctcaacc tgttctctta taccggtagc gccaccgtgc atgcgggatt aggcggcgcg
    11701 cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg agtgggcaga acgcaacctg
    11761 cgtctgaatg gcctgaccgg gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg
    11821 ctgcgtgagg caaatgaaca gtttgatctg atctttatcg atccgccaac cttctctaac
    11881 tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atatggcgct gatgaaagat
    11941 ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa acgtggcttc
    12001 cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa
    12061 acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct gattaccgca
    12121 gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg tcgttcagcg
    12181 acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt gtttgtctgg
    12241 tgggtcgcaa cggcgcgggc aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc
    12301 tggatgacgg tcgcattatt tacgagcaag atttgattgt agctcgtctg caacaggagc
    12361 cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt gaagaacaag
    12421 cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac ccgagcgaga
    12481 aaaatctcaa cgaactggcg aaggttcagg aacagttgga tcaccacaac ctgtggcagc
    12541 tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaat gttgcgctgt
    12601 cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg gtgagtaatc
    12661 cgcgcgtgct gttgcttgat gaaccgacaa accacctgga tattgaaacc atcgactggc
    12721 tggaagggtt ccttaaaacc ttcaatggga cgattatttt catctcccac gaccgttcgt
    12781 ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg gtgacctatc
    12841 cagggaatta cgaccagtac ctgcttgaaa aagaagaagc cctgcgcgtg gaagaactac
    12901 aaaatgccga gttcgatcgc aaactggcgc aggaagaggt gtggatccgc caggggatca
    12961 aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcaatg cgtcgcgagc
    13021 gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag gccagccgct
    13081 ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttgacggt aagcaactgg
    13141 tgaaagattt ttcggcccag gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg
    13201 ggtgcggcaa aaccacgctg ctgaaactga tgctcgatca gcttcaagcg gacagcgggc
    13261 gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc gcggaactgg
    13321 atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg atggttaacg
    13381 gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg aaacgggcga
    13441 tgacgccggt acgtgcgctt tctggcggtg agcggaaccg cttgctgctg gcgcgtttgt
    13501 tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt gatgtcgaaa
    13561 cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg ctggttagcc
    13621 acgatcgtca gtttgtcgat aacaccgtta cagaatgttg gatcttcgaa ggcggcggta
    13681 aaattggtcg ttatgtcggc ggttatcatg atgcccgtgg tcagcaagag cagtatgtgg
    13741 cgctcaaaca gcctgcggtg aaaaaaaccg aagaagccgc cgcggcaaaa gcagaaactg
    13801 taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag cagctaccgc
    13861 aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaggtg gcggatgctt
    13921 ccttcttcag tcagccacat gagcagacac aaaaagtgct cgccgatatg gctgctgcag
    13981 agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta aaaaatggtg
    14041 gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa ttgcctgatg
    14101 aactgggttt atcaggcatg aaaaccaaca ctttgttagc catctgtaaa atagcgcatc
    14161 attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt
    14221 gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcgaca tgtccccggt
    14281 gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtccgacc gcctatgcgt
    14341 tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg
    14401 caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt tctgaggact
    14461 acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga
    14521 taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa gagcaactgg
    14581 cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc gctggtgtgc
    14641 tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc agctttctcc
    14701 cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat cgtcgctggt
    14761 tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca ggcgtcacgg
    14821 ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct gctgatgaac
    14881 ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc cagcagtgga
    14941 cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt ttgcccatca
    15001 tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg gtcattctgt
    15061 tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt attatggtgc
    15121 caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg catggcaagc
    15181 gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc cgctggtcga
    15241 tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga ggtttaatga
    15301 gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg acaatgtttt
    15361 ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca gagcatgagg
    15421 agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt gaagaactgg
    15481 tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt tcttttttat
    15541 cattacagcc atcagggact ggaagtgacc ctgatcaccg cgaatgcgga aggaattgaa
    15601 ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga aagcgccaca
    15661 ctggctgatg atttgacgca cgttgaaatc aaagtgcggc tgaattctgg tatggaaaaa
    15721 ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg cgaagggatt
    15781 agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg cgcgaaaggc
    15841 agcaaaatgg ataaatacga tttgctggac tcgccaccgt tggccccgcc tgatgcgaaa
    15901 ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg agatccggtg
    15961 ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac gcaaaaacgc
    16021 aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac cagcaatgtt
    16081 cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat gcgtgtggag
    16141 atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc ggaaggtctg
    16201 gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga tcagaagagc
    16261 attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa agattcggta
    16321 cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg taccgtaagc
    16381 aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta ccgtattccg
    16441 gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt
    16501 gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac
    16561 ctggtcactg gtgcactgta tgttgatctt gatttctatc caaatacgcc tgcaataacc
    16621 ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg cctggcgcaa
    16681 atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt gaatccgatg
    16741 attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg
    16801 acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt gccgacggat
    16861 atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc tggctccgca
    16921 gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg agaactgcaa
    16981 ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa
    17041 aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg attgcagcac
    17101 tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg
    17161 tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg gtagagcagg
    17221 tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt
    17281 atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacaattg cgcaacaccc
    17341 tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag cctctgggaa
    17401 gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat gatggcaagg
    17461 tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa cgtccgttcc
    17521 gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagta ctggccagtg
    17581 tctggagtca ggaagccgct tctattgcgc aagagataaa gcgtctacct taattataaa
    17641 gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg tttctaacgt
    17701 gcggaaaaag ttgttcctct ttacattttt tgtacaaccg acatacccgt gtagctcaca
    17761 aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg gtattgctta
    17821 actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca gtatgaggga
    17881 aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa cgtggttatc
    17941 aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg aatcaaaggt
    18001 cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg gcctgattct
    18061 gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac tctgtctgat
    18121 tataatcaga aggcagaagt atcctggaac agaccgactt tcaggtcgct ggcggtatag
    18181 atcaaacgac catcaaccag cacttctcca tccgccaggc ccataatcag acgacggtta
    18241 acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag tacctgacca
    18301 gtgaatttca cttcgccaac gcccagtgcg cggcctttac cttcgccgcc cagccagccg
    18361 aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg cataaccgga
    18421 tcgccaataa agtggcatcc gaagaaccac agatccggat tgatatccag ttctgcttca
    18481 acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg gtccatcatc
    18541 agcatattcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc gcgaccagag
    18601 gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc tgtaagcctt
    18661 attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc gatcagttcg
    18721 gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct tcctgttgcg
    18781 atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct tcgccatccc
    18841 acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc cagatggtga
    18901 atttgccttc ttctaccgct ttcactagtt cactgtgaag acttaaatgg cgaacgttag
    18961 ctgtggggat aatgacacct tgtttcccgg ttaactcacg ttgctggcaa atagcaaaga
    19021 agccttcgat tttctcattt aaaccaccga ccggctgggc gcgaccgaac tgatcgactg
    19081 aacctgtgat agcgatactc tgattcaccg gcacatcggc gagggcgctt atcagggcgc
    19141 agagttcagc catcgaggca ctatctccat caacttcact gtatgactgc tcaaatgtca
    19201 gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg aacgcttgca
    19261 tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg
    19321 tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc
    19381 gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt
    19441 cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca gcgaggaaac
    19501 cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag aaggtgtcgc
    19561 catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg ctaagcggaa
    19621 gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc caggcatccg
    19681 ctcccggtgc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga caccactggg
    19741 ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa atagcctgct
    19801 ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg cctaccagaa
    19861 tgactttcag cttcaatggc atcgaaggca cagagacggg gagagggcgc gactcatcaa
    19921 acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc cacagcagag
    19981 gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc tgatgcacca
    20041 gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag agttgctccg
    20101 cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca tctgcactca
    20161 ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgaccgccc accagttggc
    20221 ctgcatcgct ttgtagcgta cgcgcggcat tggcaataag attcagatac tcagactctt
    20281 ccggggcctt cgccagcata aaagaggagg atgctcgcgt atgcagcaac tgctccagcg
    20341 caaattgcag ccgcggttga gtatcactga ataaaggatc gttttcgtca atcaaatgtg
    20401 gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc catgcaagtt
    20461 tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt ataccgtaac
    20521 cggcactaca gcacacgtag aaagcaccga caatactcca ggcatgggcg ttaaagctca
    20581 caggatggag attcttttct tcactggcct aaaaagctga tattctgtaa agagttacac
    20641 ggtaacattg agatcgctat gaaatatcaa caacttgaaa atcttgaaag cggttggaaa
    20701 tggaagtatc tggtgaagaa gcatcgcgaa ggggagttaa tcacccgtta catagaagcc
    20761 agtgccgccc aggaagccgt cgatgtgttg ctctcgctgg aaaatgaacc cgtgctggta
    20821 aatggctgga ttgacaagca tatgaatccg gaactggtca atcgcatgaa gcaaaccatt
    20881 cgggcaagac gaaagcgcca ttttaatgcg gaacaccagc atacgcgcaa aaaatcgatc
    20941 gatctggaat ttattgtctg gcaacgtctg gctggtcttg cgcagcgtcg cggtaaaacg
    21001 ctttctgaaa cgattgttca gctgattgaa gatgcggaaa acaaagagaa atacgcgaat
    21061 aaaatgtctt ctctgaagca ggatctgcag gcattgctgg gtaaggaata actgacgaaa
    21121 gtcagttcaa tttactaaag gcaaaaaaaa ccccgcagca gcggggtttt tctaccagac
    21181 gagaacttaa gcctgcggct gagttacaac gtctttgata cctttaactt cgatctctac
    21241 gcgacgatcc ggagccaggc agtcgatcag tgcagcacgc tgtttcacgt tgtcacaggt
    21301 gttgccagta accgggttgg attcgcccat accacgtgcg gagatcttgt ctgccgggat
    21361 acctttggag atcaggtaat caacaacaga ctgagcacgg cgctcggaca gaccctggtt
    21421 gtaagcgtca gaaccgatgc ggtcggtgta acccagaaca actacggaac cgtctttcgg
    21481 atccaggttg ctcagctggc tgtacagctg atccagagca gcctgacctt ccggtttcag
    21541 ggttgctttg ttgaagttga acagaacgtc agacttcaga gtgaagtgct tggtctgtac
    21601 ttccggtgcc ggagctggag ccggagcaac tactggagct gcttcgccct gaccgaaacg
    21661 gtaggaaacc cccaggctca gcatgccgtt gtccggacga gtgccgatgg tgtgtgcgtc
    21721 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    21781 ctcaacaccg ccagcgaaga ccggagaaac gccggtgtcg tggtttttac cataaacgtt
    21841 ggatttagtg tctgcacgcc ataccatacc acccagacga gtgtagatgt ccaggtcgtc
    21901 agtgattggg taacccagtt tagcggtcag ttgaacgccc tgagctttgt atgcaccgtt
    21961 ttcaacgctg cctttgtacg gcatacgacc taaccagtcg taacccattt caaagccaac
    22021 atacgggtta acctggtaac caccaaaagc accagcgccc agttggtttt catgggtcgg
    22081 gccattgttg ttgatgaaac cagtgtcatg gtactgggac cagcccagtt tagcaccagt
    22141 gtaccaggtg ttatctttcg gagcggcctg cgctacggta gcgaaaccag ccagtgccac
    22201 tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg ttatcatcca aaatacgcca
    22261 tgaatatctc caacgagata acacggttaa atccttcacc gggggatctg ctcaattaac
    22321 tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct acaacgtagt
    22381 tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg tataaggtat
    22441 gtttaatctt ttttgtcagc gacaatttac agaagaaaat cgcggaaacc gcttcagaca
    22501 agcctccgca aggaaaatta gtcacgactg aaagcattgg ctgggcgaca aaaaaagttc
    22561 caggattaat cctaaattta cttaatgata caaattagag tgaattttta gcccggaaag
    22621 ttgtctcgtg gcgtgagagg atgcgcttac cggacgcata ataaacccca tagcgttacc
    22681 ttcatttgcc gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat ctgccaacca
    22741 accgatcacc acactgtaat tgcccgtgcg taaagcgcga accattgact ccacagtgtg
    22801 gcaaggggag agctggctaa tctgcattac tttcgttaag ggtagcccag atgcctgaac
    22861 ccattcccga ctcagttttt gttgcggtgt taaccagagt tgccagcgcg attgctgacc
    22921 gagttgctgt aacaatggca acagtagaag ttgcgtcatc atgggctgat cttcgcgata
    22981 gacaacttca ctgataagcc cggctgtagt gttttccgta gagacacgcg caattttact
    23041 tgctgcggat gagaacgacg aagaacgatg tgcatagcct gaagtgtaca taatcaatcc
    23101 agcccctatg agttactgta tggatgtaca gtacatccag tgacaacaaa gatcaaccct
    23161 attttcggaa agagcctcgc aaattttgtc gttggtgacg ggaaaacata aattaatctt
    23221 gccccttaag aataagttgc ctattttcgt agttaacgga tccgttaatg tgaatcattc
    23281 ttttatgtta tgattttaaa aggaatttta tgaaaagcct ctcctataag cggatctata
    23341 aatcacaaga atacctggca acgttgggca caattgaata ccgatcattg tttggcagtt
    23401 acagcctgac cgttgacgac acggtgtttg cgatggtttc tgatggtgag ttgtatcttc
    23461 gggcttgtga gcaaagtgca cagtactgtg taaaacatcc gcctgtctgg ctgacatata
    23521 aaaagtgtgg ccgatccgtt accctcaatt actatcgggt tgatgaaagt ctatggcgaa
    23581 atcaactgaa gctggtgcgt ctgtcgaaat attctctcga tgcagcgctg aaagagaaaa
    23641 gcacgcgcaa tacccgggaa agactgaaag atttgcccaa tatgtctttt catctggaag
    23701 cgattctcgg ggaggtgggg attaaggatg tacgggcgtt acgtatactt ggggcaaaaa
    23761 tgtgttggtt gcgactgcgg cagcaaaaca gtctggtgac agaaaagatt ctgtttatgc
    23821 ttgaaggtgc cattatcggc attcatgaag ctgcgctccc ggtggcacgc cgccaggagc
    23881 ttgcagaatg ggctgactct cttacgccga aacaggagtt tcctgcggaa cttgagtaat
    23941 ctggcgttgc agacgaccaa tctcaggcag taatgcaatc aataatccaa cttgttgtac
    24001 gaccagaggt tctttgctgt ctgcccgtgg ttcaagttgc tgcatccgct gtttcaggct
    24061 agctaatgcc tcattgacgc gttcttcatc agcaggttga tgatgtaacg cgtcatcaac
    24121 atagcaaact gcgtcatcaa gaaacgccag aatttcagga ttagttaact gctcccggtg
    24181 agcaccgagg gctgagatat agctggtaaa cgtatggtta aggcacagca accgaaacgc
    24241 ggcttcgcga atttgcgggg taacgttcgg ctcgctggac atatttgata ccaccgacgc
    24301 cagctcagca tcacggttgt gtgcatcgcg gcgggcaata cgatacgcca gacggttatc
    24361 acgcccctga tggtattgct ccagtatggc atcgagatac cgacagttgg cctctgtggc
    24421 gcgttcgagc atgcgcggca gattgcgaaa ctgccagtca ggccagatgt agctcactgc
    24481 cgcccacgca atggcacaac caatcagcgt atcgattacg cgaggtaacg ctacttcaaa
    24541 accttcaccc agtaagttaa aacacagtag caccaaaagt gtgatgaaca tcgttgcatg
    24601 agcgtattgc acgttacgga aggcaaaaaa gagcacgccg gtaataacca gcagcaccag
    24661 ctgcccttcc agtgatggca caaaccacag cacaggaatg ccaatggcga tacctaccag
    24721 cgtaccaata atccttaact tcaggcggtg gcgcgtggcg ttatagtttg gctggcagac
    24781 aaacaaactt gtcagcaaga tccaataccc gtgatgcatt ccggttatct gaatgatggc
    24841 gtagccgaag cacaatacca gcgacattct taccgcatga cggaagaggg cggattccgg
    24901 cgtgaagtga cggctaagac gcagccagat atcactcaac ccgtgcgggc tgtcatcagc
    24961 gagctcattt tcgtcattat tatggggtag tgcctgggcc tgttctgatt caattgttgc
    25021 cagttgggca tcaatggcgc gtaaattgtt cagcaaaaat cccagtgttt tgagtaaatc
    25081 ggcgggtgcg ccgttatcgc gcatccgctc cagcgcagca tcaatatgcg taaaagcgcg
    25141 ctcaaaatgc ggatcatgtt gataaggctg acgcaacaaa atacagcgtg acagttgctg
    25201 gcacgcctgg ccctgcatcg acatcagccg ctgaaaacgg aacagcacgt cgctgtggcg
    25261 aaaatgttca cgcaatgttt gatactgaat atgagaagag ctggcacgct cgtgaatatc
    25321 ctgtgcgaca aagtaataat gcagcgtgcg acgcgttccc cgttgaccac gatcgccacg
    25381 taagcgggtc agcagcgaga gtttcgtctg attcaatgtc gccatcagca gaccgttggc
    25441 gagagccaaa tcgtacagcg gtgcctggct ttcatcttca atatcaggat caaacatgcg
    25501 cgacttgagc tcaagataac gcgccagttg ttcatagcaa cgcgccaggt tgtcctgcag
    25561 cgggcggacc gggaacagca gatgaccaat aagtgtcagg acgttgtacc agacggcacc
    25621 ggccagcaga tacatcggct gctgatacca gtgctcatac agtgatgttc ccaacatagt
    25681 gtaaatggcg atcagcaatg caccgaaggc aattgttgca tagcgttgac ccagaccgcc
    25741 gagcaaaatg aagccgctgg tagagagcgt taagccaatc gcaaatagcc agggccaggg
    25801 aaacagcaat tctactgagg ccgaggcgat aaaaaagcag aacagcgtaa tgatgaggtt
    25861 acgcaaacgt cccgccagtc ggtcatcgag atcggtcagc gccgctgcca ccatccccag
    25921 cgttagcgga atcgtcagtt ttacatcacc cagccaccac ggaaacgctg tggttccaca
    25981 aagcgcaata aaaatacgcg cgtaatacag ccaggcgctg ttccaggtat agcgtttgag
    26041 caaaggactt agcataaagg ccatattagt ggattattca aaacgacgac gtgcattggc
    26101 ttcacgcgca gcttgcgctg tttctaccga taccacgcga cgaccaaccg gccatagtgc
    26161 aatagcggca attttaaagt tcgcaatgcc gacaggaatg ccaatgattg aaatacattg
    26221 tgcgatgccc gttgcaatgt gcatcaggca taaccaccag ccaaagaaaa tcagccagaa
    26281 aatattcaat accgtaccgc cagtattcag cagcacattt ttgccagccg ggttcagttc
    26341 atcgacatga atagcttcat tgccataagg caccagagat agtttagtga tctcccagca
    26401 ggatcgtgtc agcggtaagg taaaaatcag cacaatactg accagagtcg ccaacagcca
    26461 gcccagagtg gtggcaaatc cgccaagcac aaagttcaga atgttcaaaa cggtacgcat
    26521 aaaacctcgc tttactgtgg ttttcagtaa tgggcggcaa ttgtaacgtt tttttgggct
    26581 ggagcacgtt ttctctgacg gttacactga taagaaataa tttcgtgtgg atctacagag
    26641 tcatggaact gaaagcgaca acgcttggaa aacgtctggc acagcaccct tacgatcggg
    26701 cggtgatcct caatgccggg attaaagtct ccggcgatcg ccacgaatac cttattcctt
    26761 tcaatcaatt actggcgatt cactgtaagc gcggtctggt atggggcgag ctggaatttg
    26821 tactgccgga cgaaaaagtg gtgcgtctgc acggcaccga atggggcgag acgcagcgtt
    26881 tttaccatca tcttgatgct cactggcggc ggtggagtgg cgagatgagc gaaattgcgt
    26941 ctggtgtttt acgccagcaa ctggatttga ttgccacgcg cactggagaa aataaatggc
    27001 tgacgcgtga gcaaacctct ggcgttcagc aacaaatccg ccaggctttg tcggcgttgc
    27061 cgttgccggt taaccgactg gaagaattcg ataactgccg tgaggcgtgg cgtaaatgtc
    27121 aggcctggtt gaaagatatt gaaagcgctc ggttgcagca taaccaggcg tataccgaag
    27181 ccatgcttac cgagtatgcg gattttttcc gccaggtcga gtcttcaccg ctgaatccgg
    27241 cgcaggcccg ggcagtcgtt aatggcgagc attctctgtt agtgctggca ggtgcaggaa
    27301 gcggaaaaac gtcggtgctg gtggcccgtg caggctggtt gctggcgcgt ggtgaagcgt
    27361 cccctgagca aattttattg ctggcgtttg gtcgcaaagc cgctgaagag atggacgagc
    27421 ggattcgcga acggctacat accgaagaca ttaccgcacg cacgtttcat gcgctggcgc
    27481 tgcatattat tcagcagggc agcaaaaaag ttccgatagt cagcaaactg gaaaatgata
    27541 ccgctgcccg tcatgaactc tttattgctg agtggcgcaa gcaatgcagc gaaaagaaag
    27601 cgcaggcgaa gggctggcgg caatggctga cggaagaaat gcagtggtca gtgccagaag
    27661 gtaacttctg ggatgatgaa aaattacagc gtcgccttgc ctctcgcctc gatcgctggg
    27721 taagtctgat gcggatgcac ggtggtgcac aggcagaaat gattgccagt gcacccgaag
    27781 agattcgcga tctgttcagt aaacgtatca agttgatggc cccgttatta aaagcctgga
    27841 aaggtgcgct gaaggcagaa aacgctgtcg atttttcggg ccttattcat caggcgattg
    27901 tgattctgga gaaaggtcgc tttatcagcc cgtggaagca tattctggtt gatgaatttc
    27961 aggatatctc gccgcagcgg gcagcgttgt tagcggcatt acgcaagcaa aacagtcaga
    28021 cgacgttgtt cgctgttggt gatgactggc aggcgattta ccgattcagc ggtgcgcaaa
    28081 tgtcgctcac caccgctttc catgaaaact ttggtgaagg cgaacgttgt gatttagaca
    28141 cgacttaccg ttttaacagt cgtatcggta aggtggcaaa ccggtttatt cagcagaacc
    28201 caggccagct gaaaaagccg ctaaacagct taaccaatgg agacaaaaaa gccgtcacgt
    28261 tattggatga gagtcaactt gacgctttgc tggataagct ctctggttat gccaaaccgg
    28321 aagagcgcat tctgatcctg gcgcgttacc atcacatgag gcctgccagc ctggaaaaag
    28381 cggcaacacg ctggccgaag ttgcaaatcg actttatgac cattcatgcc agcaaagggc
    28441 aacaggcgga ttacgtcatc atcgttggct tgcaggaggg aagtgatggt tttccggctg
    28501 cggcgcggga gtcgattatg gaagaggcgc tactgccacc ggttgaggat ttcccggacg
    28561 ctgaagaacg gcggttaatg tacgtggcgc tgacccgggc acgccatcgg gtatgggcac
    28621 tgtttaacaa agagaatccc tctccctttg tggaaatact gaaaaatctg gatgtgccgg
    28681 tggcgagaaa accgtaagaa acaggtggcg tttgccacct gtgcaatatt acttcagacg
    28741 gtccgcgaga taacgctgat aatcggggat tagaatatcg accgcgtcgt tgaaatgcgg
    28801 cgactggatt atgaagtctg ccgttgccac gttggtggcg accggaatgt tccataccgt
    28861 cgccagacgc agcaaggctt tcacgtcagg atcgtgcggc acggcattta gtggatccca
    28921 gaagaaaatc aatacatcaa ttttcccttc tgagatcaat gcgccaacct gctggtcacc
    28981 ccccattggg ccactcaaca tcgcgttgac gttcatgccg gtcgcgcggg aaattaagtt
    29041 accggtagtg cctgttgcat acagtacgtg ttgttccagt aacggttgat gccgttccac
    29101 ccagctcatc agcatttgtt tgcagtgatc gtgtgccacc agcgcaatat gtttccgcgc
    29161 aggtaaagtg cgagtcgtca gttccataat gtacatccgt agttaacttt cctacagatt
    29221 actgtaagca cttatcgctg caagataaag accgaaaaag cctgcgcaca ggcacaaaaa
    29281 tctcaggaag atggttgttt ttccgcccac tgcaggaaag tatttcgcgt ttgtgggtca
    29341 gccagtttaa accaatactt cagccgttgt tctgtgagca cctgagactg cggtggaata
    29401 gcatccagct cggaaacacc cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat
    29461 tgcggtagtg aagcgcgttt cgcggatttg ttatagcgtt caacctcgac ctcataatca
    29521 atcgtttttg ccgttgaggt aatggcgaga atatccagct ttaccggaat cggcgtcgca
    29581 tcgccatcca acaattcaag gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc
    29641 tccaggcgag gcaggcgaaa attgacctgg ttgatgagct gggtattaaa actgaccacc
    29701 agcggtgggg agatatacag ccgttcttca ctattggaaa gatgaatcgt cttctcaacg
    29761 cgaaacacta actgatgcgg tccattatcc agttcaatgc tgtccgcacc gcgcagcaga
    29821 gagctggaaa ccttcttgcc gtcgagtacc agaagatcga catcggttga aagccgcagc
    29881 gtggtggcaa aaacggatac cggcaaacat aatgcaatta aggtggtcac gatgccggtt
    29941 ttcataggta gctcctgtca aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta
    30001 atca
//
LOCUS       DAJTVJ010000043        29550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000043 DAJTVJ010000000
VERSION     DAJTVJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.043"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..690
                     /gene="dgt"
                     /locus_tag="OO448_001859"
     CDS             <1..690
                     /gene="dgt"
                     /locus_tag="OO448_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCR6337076.1"
                     /translation="LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSR
                     SNSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDL
                     LKLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKR
                     FPIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAW
                     DEYRRLMAVEQ"
     gene            820..2244
                     /gene="degP"
                     /locus_tag="OO448_001860"
     CDS             820..2244
                     /gene="degP"
                     /locus_tag="OO448_001860"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6337077.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2399..3556
                     /gene="cdaR"
                     /locus_tag="OO448_001861"
     CDS             2399..3556
                     /gene="cdaR"
                     /locus_tag="OO448_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR6337078.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3645..4031)
                     /locus_tag="OO448_001862"
     CDS             complement(3645..4031)
                     /locus_tag="OO448_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6337079.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="OO448_001863"
     CDS             complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="OO448_001863"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR6337080.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="OO448_001864"
     CDS             complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="OO448_001864"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6337081.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="OO448_001865"
     CDS             complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="OO448_001865"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6337082.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8649..9443)
                     /gene="map"
                     /locus_tag="OO448_001866"
     CDS             complement(8649..9443)
                     /gene="map"
                     /locus_tag="OO448_001866"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6337083.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9811..10536
                     /gene="rpsB"
                     /locus_tag="OO448_001867"
     CDS             9811..10536
                     /gene="rpsB"
                     /locus_tag="OO448_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6337084.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10794..11645
                     /gene="tsf"
                     /locus_tag="OO448_001868"
     CDS             10794..11645
                     /gene="tsf"
                     /locus_tag="OO448_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6337085.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11792..12517
                     /gene="pyrH"
                     /locus_tag="OO448_001869"
     CDS             11792..12517
                     /gene="pyrH"
                     /locus_tag="OO448_001869"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6337086.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12809..13366
                     /gene="frr"
                     /locus_tag="OO448_001870"
     CDS             12809..13366
                     /gene="frr"
                     /locus_tag="OO448_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6337087.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13458..14654
                     /gene="ispC"
                     /locus_tag="OO448_001871"
     CDS             13458..14654
                     /gene="ispC"
                     /locus_tag="OO448_001871"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6337088.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14843..15601
                     /gene="ispU"
                     /locus_tag="OO448_001872"
                     /gene_synonym="uppS"
     CDS             14843..15601
                     /gene="ispU"
                     /locus_tag="OO448_001872"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6337089.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15614..16471
                     /gene="cdsA"
                     /locus_tag="OO448_001873"
     CDS             15614..16471
                     /gene="cdsA"
                     /locus_tag="OO448_001873"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6337090.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16483..17835
                     /gene="rseP"
                     /locus_tag="OO448_001874"
     CDS             16483..17835
                     /gene="rseP"
                     /locus_tag="OO448_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6337091.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17865..20297
                     /gene="bamA"
                     /locus_tag="OO448_001875"
     CDS             17865..20297
                     /gene="bamA"
                     /locus_tag="OO448_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6337092.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20419..20904
                     /gene="skp"
                     /locus_tag="OO448_001876"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20419..20904
                     /gene="skp"
                     /locus_tag="OO448_001876"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6337093.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20908..21933
                     /gene="lpxD"
                     /locus_tag="OO448_001877"
     CDS             20908..21933
                     /gene="lpxD"
                     /locus_tag="OO448_001877"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6337094.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22038..22493
                     /gene="fabZ"
                     /locus_tag="OO448_001878"
     CDS             22038..22493
                     /gene="fabZ"
                     /locus_tag="OO448_001878"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6337095.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22497..23285
                     /gene="lpxA"
                     /locus_tag="OO448_001879"
     CDS             22497..23285
                     /gene="lpxA"
                     /locus_tag="OO448_001879"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6337096.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23285..24433
                     /gene="lpxB"
                     /locus_tag="OO448_001880"
     CDS             23285..24433
                     /gene="lpxB"
                     /locus_tag="OO448_001880"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6337097.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24430..25026
                     /gene="rnhB"
                     /locus_tag="OO448_001881"
     CDS             24430..25026
                     /gene="rnhB"
                     /locus_tag="OO448_001881"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6337098.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25063..28545
                     /gene="dnaE"
                     /locus_tag="OO448_001882"
     CDS             25063..28545
                     /gene="dnaE"
                     /locus_tag="OO448_001882"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6337099.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28558..29517
                     /gene="accA"
                     /locus_tag="OO448_001883"
     CDS             28558..29517
                     /gene="accA"
                     /locus_tag="OO448_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6337100.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8160 g         7110 t
ORIGIN      
        1 cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca tcatttgcac
       61 gaggcgtggg gtcagcatga gaaaggttcg ctcttttcgc tagtggttga aaatgcctgg
      121 gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt tatgtattta
      181 cgggtaaaca ccctaaataa actggtaccc tacgcggcac aacgatttat tgataatctg
      241 cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag cgaatgcagc
      301 gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca tccggatgtc
      361 gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat ttatcgtcct
      421 ttattaagcc tgtcgttatc agactttact gaactggtag aaaaagaacg ggtgaaacgt
      481 ttccctattg agtcgcgctt attccacaaa ctctcgacgc gccatcggct ggcctatgtc
      541 gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga atattattac
      601 cgttgccgcc tactgcagga ttatatcagc ggtatgaccg acctctatgc gtgggatgaa
      661 taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga acaataaatt
      721 tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat ctgaagaaca
      781 cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac cacattagca
      841 ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc aacggcggct
      901 gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat gctcgaaaag
      961 gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa tacgccgcgt
     1021 atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca ggaaggttca
     1081 ccgtttcaga gctctccgtt ctgccagggg ggccagggcg gtaatggcgg cggccagcaa
     1141 cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg ctatgtcgtc
     1201 accaacaacc atgttgttga taacgcgacg gtgattaaag tccagctgag cgatggtcgt
     1261 aagttcgacg cgaagatggt tggcaaagat ccgcgctctg atatcgcgct gatccaaatc
     1321 cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact gcgcgtgggt
     1381 gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac ttccgggatt
     1441 gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt catccagacc
     1501 gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa cggcgaactg
     1561 atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat cggttttgct
     1621 atcccgagta acatggtgaa aaacctgacc tcgcagatgg tggaatacgg ccaggtgaaa
     1681 cgcggtgagc tggggattat ggggactgag ctgaactccg aactggccaa agcgatgaaa
     1741 gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc cgctgcaaaa
     1801 gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat cagcagcttt
     1861 gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac cctgggctta
     1921 ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag ccagaatcag
     1981 gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa caaaggcaaa
     2041 gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca gatcggcctg
     2101 aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat cgctgaactg
     2161 cgtaaagtgc tcgacagcaa accgtctgtg ctggcactga acattcagcg cggcgacagc
     2221 accatctacc tgttaatgca gtaatctccc tcaacccctt cctgaatacg ggaaggggtt
     2281 ctccttacaa tctgtgaact tcgccacaac tccatacatc ttcatcatcc tttgggcatt
     2341 tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg gaggacttat
     2401 ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat
     2461 catcgatacc aatatcaacg taatggatgc ccgcgggcga attatcggca gcggcgatcg
     2521 tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg gacgagtcgt
     2581 cgatatcgat gacgcggtag cacgtcatct gcacggtgtg cggcagggga ttaatctacc
     2641 gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac cagagaatct
     2701 gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac agtcgcggtt
     2761 gatgcacctg ttggcgcagg atagccgttt gcgggaagaa ctggtgatga acctgattca
     2821 ggctgaggag aatactcccg cacttactga atgggcgcaa cggctgggga ttgatctcaa
     2881 tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg tggacagcgc
     2941 aatggcggag ttacaacaac tgcaaaatgc gctgactacg cccgagcgta ataatctggt
     3001 ggcaattgtt tcgctgaccg aaatggtggt gttgaaaccg gcgttgaact cttttgggcg
     3061 ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca tgaaagagta
     3121 cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg gcagtattgc
     3181 ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga tgccagaaag
     3241 tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga
     3301 ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg acaataatgg
     3361 cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc tggctacgtc
     3421 aaaggcgctg tttattcatc gtaatactct ggagtatcgg cttaatcgta tatcggaact
     3481 gaccgggctt gatttgggta attttgatga caggttgctg ctgtatgtgg cgttacaact
     3541 ggatgaagag cggtaggtta tgcgttaagg tcgaggcggc gctggctcat tcccctcagc
     3601 ccgaagggta gaggggaatg tccgagttaa ccgtcagttc ggcattattt acgcgttagt
     3661 ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc caggtgacgt
     3721 aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg gcagatttga
     3781 tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc tttataaccc
     3841 tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa cttcacgctc
     3901 ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc gttgttggct
     3961 tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag acttttcaga
     4021 ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg
     4081 catggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga cttctatcat
     4141 ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg ttctacacca
     4201 gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa ctgcccacgc
     4261 cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg taacgtttat
     4321 cttcgacagg ggcaaaaggt tcaccaacca acggtacgcg cagttgcccg ccgtgagtat
     4381 ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg tcgggattat
     4441 gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg cattgtccgg
     4501 cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg atcaccgtag
     4561 cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt aagtgatttt
     4621 tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc gcacattcgg
     4681 caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca aataatacgt
     4741 aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca atcgcgtcag
     4801 aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa agaattttga
     4861 atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca aaccagccta
     4921 gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg gcagtagcct
     4981 gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg gcagcgtcgt
     5041 gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg ccgactttgc
     5101 cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg tatttgccat
     5161 cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgcga ccgtagtgga
     5221 tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg cccatggaaa
     5281 tgacggaacc ttcttcgaca atcacccctt caaccacttc agagcgtgcg ccaatgaagc
     5341 agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg ccgatgccca
     5401 cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg
     5461 tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac ggcatcagca
     5521 cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca acgcggaagc
     5581 cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt catcggcact ttgtcgaagt
     5641 agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag agcagcaccg
     5701 cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct acacgcagtg
     5761 caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg gtgtctgcgt
     5821 tggctggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc tgtaactgct
     5881 gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt ggattgaggg
     5941 cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagtgca cgccggtcag
     6001 cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt cgggcaccat
     6061 gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt cctggctggt
     6121 cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggttggcaaa aacgtcactt
     6181 cggtttcaac agtaaagtga cgtagtttag ccggttgacg acgcggctgt ggtggctgcc
     6241 agctactttg tgtcagtacc tgctccagac caaaccgaat aacctcatga cgatctgcgg
     6301 atagcgggct accatcgggt tccagcacga tgaaggtatc catcgccata ccgtcgcgag
     6361 tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg gcacagacgg
     6421 cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct ccacgcgtag
     6481 cctgcgggct aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa tggcgggcat
     6541 gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag cgtgaccaaa
     6601 tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt gcctggagtt
     6661 ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt cgtagctgct
     6721 tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc cacagcgttt
     6781 cgttggtggc gcaaatgtca gccactgtca ggcataccag atagtgcaga cgattttccg
     6841 tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg cggcgttgtg
     6901 ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt gtttcgcgtg
     6961 agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga atggagtggt
     7021 cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc agctcagttg
     7081 acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt tcttcactgg
     7141 caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg taggcgtgga
     7201 acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac gcgccgagca
     7261 cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga cgcagaatgc
     7321 tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga tggcgacggg
     7381 catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg ttgcgcacca
     7441 tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt tcatcacgca
     7501 ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt ttttcgtcgg
     7561 cgggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg agttcactga
     7621 cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg ttgccttcac
     7681 cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg cgattatcgt
     7741 aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc agacattcgt
     7801 ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca tccagcgatg
     7861 ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc aagccgccag
     7921 ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc tgatggcgct
     7981 ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat ccttcgctaa
     8041 aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag cgggattcga
     8101 ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct tcaagcgtgc
     8161 gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc tcgcccactt
     8221 tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg atgtcagaaa
     8281 gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg tcggcaatct
     8341 ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac tcggtgcgcg
     8401 cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc caacgctgga
     8461 aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc cagacgcatg
     8521 gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt tctggaaggg
     8581 tattcattgt gcgccaccca taagattaaa ttatcgcatt aaaaaagccg gcattcgccg
     8641 gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc
     8701 agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc agacaagctg
     8761 cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt tttacccgcg
     8821 ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt ggtttcacgg
     8881 gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc gtgtccgcaa
     8941 tattcacgaa cgacggagaa gccttctgct tcgataaact tctgaatcgc cgcaccgatt
     9001 tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct ttcttgcgtg
     9061 atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat tttcgaggta
     9121 tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc gccatctttc
     9181 agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga gatgcaaacg
     9241 gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc attaacaatg
     9301 taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac atacggttcg
     9361 atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc gatatcttct
     9421 ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata ccgacaatat
     9481 atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg
     9541 ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg ctgtacacag
     9601 cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg gacttccgat
     9661 ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa caacacacac
     9721 gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac gtggaggcat
     9781 aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg cgacatgctc
     9841 aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat gaagccgttc
     9901 atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt accgatgttc
     9961 aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt
    10021 ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg cgaccagttc
    10081 ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc
    10141 atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga aaagctgacc
    10201 aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag cctgggcggt
    10261 atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga ccacgaacac
    10321 attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt tgataccaac
    10381 tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg
    10441 accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca ggatctggct
    10501 tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac tcccccaaaa
    10561 tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac atcagtaagt
    10621 gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg aaatatgaag ggggagagcc
    10681 cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc ctttttcact
    10741 tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt agaatggctg
    10801 aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc atgatggatt
    10861 gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa aacatgcgta
    10921 agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac ggcgtgatca
    10981 aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag actgacttcg
    11041 ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca gctgttgctg
    11101 gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt gttgcgctgg
    11161 tagcgaaaat tggtgagaac atcaacattc gccgcgttgc tgcgctggaa ggcgacgttc
    11221 tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa ggcgctgacg
    11281 aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa ttcatcaaac
    11341 cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg gatatcgcga
    11401 tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc atgaagaaat
    11461 tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc aaaactgttg
    11521 gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc gaagtggggg
    11581 aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg tccaagcagt
    11641 cttaattatc gaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat cttgtaaatt
    11701 cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag tattaattca
    11761 tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac ccgtctataa
    11821 acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct tcggtattga
    11881 tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac tgggtattca
    11941 ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg cgaaagcggg
    12001 tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga acggcctggc
    12061 aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg ctattccatt
    12121 gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc gcaacaaccg
    12181 tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact cagcagcttg
    12241 cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag ttgacggcgt
    12301 gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac tgacttacag
    12361 cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc tggctcgtga
    12421 ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc gccgtgtggt
    12481 aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga taaataaggg
    12541 taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt attgttgcag
    12601 gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt gaggatggcg
    12661 agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac gattacccgt
    12721 aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg tctggtctga
    12781 ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag atgctgaagt
    12841 acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac gcacgggtcg
    12901 tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc cgacgccgct
    12961 gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca acgtgtttga
    13021 tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg gcctgaaccc
    13081 gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag aacgtcgtaa
    13141 agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag tacgtaacgt
    13201 gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga tcagcgaaga
    13261 cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa tcaagaaaat
    13321 tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc ttgaacgaca
    13381 aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt ttattctgtc
    13441 tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt
    13501 gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg
    13561 caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg
    13621 taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc
    13681 gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg
    13741 atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc
    13801 gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt
    13861 ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg
    13921 ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc
    13981 aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat
    14041 tgcgcgattt ggcaacaatg acgcccgatc aagcctgccg tcatccgaac tggtcgatgg
    14101 gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag
    14161 cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag
    14221 tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg
    14281 atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga
    14341 agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt
    14401 atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg acgacagcat
    14461 tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg
    14521 atatcgctgc gttgaattta tctgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg
    14581 tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc
    14641 gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta gcgtagggct
    14701 tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg tcaggtatgc
    14761 cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac cgtaaaccct
    14821 gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg aaaaattgcc
    14881 agcgcatggc tgccgtcatg ttgcgatcat tatggacggc aatggtcgct gggcaaaaaa
    14941 gcaagggagg attcgtgcct ttgggcataa agccggggca aaatccgtcc gccgggctgt
    15001 ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta gtagtgaaaa
    15061 ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt gggcgctcga
    15121 tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg gcgataccag
    15181 tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa cagctgggaa
    15241 taccggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata tagtccaggg
    15301 agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc agatagatga
    15361 agagatgcta aaccagcatg tctgtatgca tgaactggcc cctgtagatt tagtaattag
    15421 gactgggggg gagcatcgca ttagtaactt tttgctttgg caaattgcct atgccgaact
    15481 ttactttaca gatgttctct ggcctgattt cgatgaacaa gactttgaag gggcgttaaa
    15541 tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg aaacagcctg
    15601 atggggggcg cttttgctga agtatcgcct gatatctgct tttgtgttaa tacccgtcgt
    15661 catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc atcgtaacgc tggtggtctg
    15721 catgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt cgcagcgagt
    15781 atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt tgccggaata
    15841 tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgt tgggttggtg
    15901 gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct ggcgtaactc
    15961 taaaacattg cgccttattt ttggcgtgct aaccattgtt cccttcttct ggggcatgct
    16021 ggcgttacgg gcctggcact atgacgagaa tcattacagt ggcgcaatat ggctgctcta
    16081 tgtcatgatc ctggtatggg gcgctgactc cggcgcatat atgtttggca aattgtttgg
    16141 taaacataag ctggcaccga aggtttctcc gggtaaaacc tggcaaggct ttataggtgg
    16201 actagcgacc gcagcggtaa tctcatgggg ttatggcatg tgggcgaatc tcgacgtcgc
    16261 tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc tcggcgatct
    16321 gaccgagagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt taattccagg
    16381 acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg tctttgcttg
    16441 cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag ttttctctgg
    16501 gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga atttggtcat
    16561 ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg gtttggtaag
    16621 gcgctctggc ggcgaactga taagctcggc accgaatatg ttatggccct gattccgctg
    16681 ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga actccgccac
    16741 catgccttca ataataaatc tgttggccaa cgagcggcga ttattgccgc aggtccggtt
    16801 gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg tgtgcctggc
    16861 gtacgtccgg tggttggtga aatagcagcc aattcgatag ctgcggaagc acaaattgca
    16921 ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga tgccgtgcgt
    16981 ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacagtagc gccatttggc
    17041 agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga gcctgataaa
    17101 gaagatccgg tatcttcgct ggggattcgt cctcgtgggc cgcaaattga acctgtactg
    17161 gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg cgacaggatc
    17221 gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct tgtccgggat
    17281 aacccgggta aatccttagc gttagaaatc gaaaggcagg ggagtccctt gtctttgaca
    17341 ttaatcccgg agagtaaacc gggtaatggt aaagcgattg gttttgtcgg tattgagccg
    17401 aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc gttcaacgcc
    17461 atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc tgacggtcag tatgctggga
    17521 aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc tatcgccaag
    17581 ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct tgcgcttatt
    17641 agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg ggggcatctg
    17701 ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt tcaagacttt
    17761 tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact tttcaatgat
    17821 ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg atgaaaaagt
    17881 tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa gggttcgtag
    17941 tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggca ctcctcagta
    18001 tgccggtgcg cacaggcgac acggttaatg atgaagatat cagtaatacc attcgcgctc
    18061 tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat acccttctgg
    18121 ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac aaatcggtga
    18181 aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc gaatccctcg
    18241 atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac agcgtcggta
    18301 aatatagcgc cagcgtaaaa gctgtcgtga ccccgctgcc gcgcaaccgt gttgacctaa
    18361 aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt gttggtaacc
    18421 atgctttcac caccgacgaa ctgatctctc atttccaact gcgtgacgaa gtgccgtggt
    18481 ggaacgtggt aggcgatcgt aaataccaga aacagaaact ggcgggcgac cttgaaaccc
    18541 tgcgcagcta ctatctggat cgcggttatg cccgtttcaa catcgactct acccaggtca
    18601 gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa cattaccgaa ggcgatcagt
    18661 acaagctttc tggcgttgaa gtgagcggca accttgccgg gcactccgct gaaattgagc
    18721 agctgactaa gatcgagccg ggcgagctgt ataacggcac caaagtgacc aagatggaag
    18781 atgacatcaa aaagcttctc ggtcgctatg gttatgccta tccgcgcgta cagtcgatgc
    18841 ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt gaacgttgat gcgggtaacc
    18901 gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat gccgtcctgc
    18961 gtcgcgaaat gcgtcagatg gaaggtgcat ggctgggaag cgatctggtc gatcagggta
    19021 aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc caacgtgttc
    19081 cgggtagccc ggaccaggta gatgtagtct acaaggtgaa agaacgtaac accggtagct
    19141 tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct ggcgtgcaac
    19201 aggataactg gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa aacgattacc
    19261 agacctatgc tgaattgtcg gtaaccaacc cgtacttcac tgtagatggt gtaagcctcg
    19321 gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc gactatacca
    19381 acaagagtta tggtacagac gtgacgttgg gcttcccgat caacgaatat aactcgctgc
    19441 gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag gttgcgatgt
    19501 ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat aacagcttca
    19561 aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac cgtggttact
    19621 tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattcct ggatcggata
    19681 acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgatcgat gacgatcaca
    19741 aatgggttgt tctggggcgt acccgctggg gttatggtga tggtttaggc ggcaaagaga
    19801 tgccgttcta cgagaacttc tatgccggtg gttccagcac cgtgcgtggc ttccagtcca
    19861 atacaattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat gatccggact
    19921 atgattacga atgtgcgact caggacggcg cgaaagacct gtgtaaatcg gatgatgctg
    19981 taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg ccgtttatta
    20041 gcgataagta tgctaactcg gttcgtactt ccttcttctg ggatatgggt accgtttggg
    20101 atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat ccaagcaata
    20161 tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg ttggtgttct
    20221 cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc cagtttaaca
    20281 tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta gcgatgactt
    20341 taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg
    20401 gatggtaagg agtttattgt gaaaaagtgg ttattagcgg caggtctcgg tttagcactg
    20461 gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca atatgggcag cctgttccag
    20521 caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc
    20581 agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg
    20641 aaagcgggca gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt
    20701 gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga agaacgcggc
    20761 aaactggtta ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat
    20821 ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc
    20881 gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag
    20941 cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg
    21001 caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta
    21061 ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt
    21121 gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat
    21181 accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag
    21241 ctgggtaaca acgtatcgat tggcgctaac gcggtgattg agtccggcgt tgaactgggc
    21301 gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt
    21361 tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg
    21421 atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac
    21481 tgggtgaaga tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc
    21541 tgcacaacca tcgatcgcgg cgcgctggat gacactgtta ttggcaatgg cgtgatcatt
    21601 gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt
    21661 ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc
    21721 gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg
    21781 atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa
    21841 gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa
    21901 tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt
    21961 acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt
    22021 ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag
    22081 aacttctgcc gcaccgtttc ccgttcttac tggtggatcg cgtgctggat tttgaagaag
    22141 gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt
    22201 tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag
    22261 gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tacttcgctg
    22321 gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag
    22381 tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgatg
    22441 gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga
    22501 ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcaattggcg
    22561 cgaacgcaca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta
    22621 ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa aattggccgc gataatgaga
    22681 tttatcagtt cgcctccatc ggcgaagtta accaggatct gaaatatgct ggcgaaccga
    22741 cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca
    22801 cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaacgcgc
    22861 acattgcgca cgattgtacg gtaggtaacc gctgtattct cgccaacaac gcaacgctgg
    22921 cgggtcacgt atcggttgac gacttcgcga tcatcggcgg catgaccgca gtccatcagt
    22981 tctgcatcat tggtgcgcac gtgatggttg gcggctgctc cggtgtggcg caggacgtcc
    23041 ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat atcgaagggc
    23101 tgaagcgccg cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg tataagctga
    23161 tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa
    23221 catatccgga agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc
    23281 gttaatgact gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga
    23341 tatcctgggg gccggtttaa tccgcgctct gaaagaacat gtgcccaacg cccgctttgt
    23401 tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa gcttggtacg aaatggaaga
    23461 actggcggta atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tgcatattcg
    23521 tgccgatctg acaaagcgtt ttggcgaact gaagccagat gtttttgttg gtattgatgc
    23581 gcccgacttc aatattactc ttgaaggtaa cctcaaaaag cagggtatca aaaccattca
    23641 ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc
    23701 caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca aatacaatgt
    23761 accgtgccgc tttatcggtc ataccatggc tgatgccatg ccgttagatc cagataaaaa
    23821 tgccgcccgt gatgtgctgg ggatccctca cgatgcccac tgcctggcgt tgctaccggg
    23881 gagccgtggt gcagaagttg aaatgcttag tgccgatttc ctgaaaacgg cccagctttt
    23941 gcgccagaca tatccggatc tcgaaatcgt ggtgccactg gtgaatgcca aacgccgcga
    24001 gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctt tcagttcatt tgctggatgg
    24061 gatgggccgt gaggcgatgg tcgccagcga tgcggcgcta ctggcgtcgg gtacggcagc
    24121 cctggagtgt atgctggcga aatgcccgat ggtggtggga tatcgcatga agccttttac
    24181 cttctggttg gcgaagcggc tggtgaaaac tgattatgtc tcgctgccaa atctgctggc
    24241 gggcagagag ttagttaaag aattattgca ggaagagtgt gagccgcaaa aactggctgc
    24301 ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac gcgatgcacg ataccttccg
    24361 tgaactgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga
    24421 gttagcacaa tgatcgaatt tgtttatccg cacacgcagc tggttgcggg tgtggatgaa
    24481 gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct tgacccggcg
    24541 cgcccgattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg tctggcgctc
    24601 tatgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga accccacgaa
    24661 atcgacgagc tgaacattct tcatgcgacc atgctggcga tgcagcgtgc cgtcgctggg
    24721 ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg
    24781 cctgcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc
    24841 ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat
    24901 ggttttgccc aacacaaagg gtacccaacc gcttttcatc tggaaaaact ggctgaacac
    24961 ggcgcgaccg aacaccatcg gcgcagcttt gggcctgtca aacgcgcact gggacttgcg
    25021 tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc
    25081 gtacacctgc gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg
    25141 ttggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac
    25201 ctttgtggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcctatcgtc
    25261 ggggcagatt ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca cctgacggta
    25321 ctggcggcga acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag
    25381 cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaacgaa
    25441 gggttgatcc ttctttccgg cggacgcatg ggcgacgtcg gacgcagtct tttgcgtggt
    25501 aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat
    25561 tttctcgagc tgatccgcac cggcaggccg gacgaagaaa gctatctgca cgcggcggtg
    25621 gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacca acgacgtgcg ctttatcgac
    25681 agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac
    25741 gatcctaaac gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga agaggagatg
    25801 tgcgagctgt ttgccgacat ccccgaagct cttgccaaca ccgttgagat cgccaaacgt
    25861 tgtaacgtaa ccgtgcgtct tggtgaatac ttcctgccgc agttcccgac cggggacatg
    25921 agcaccgaag attatctggt caagcgtgca aaagagggcc tggaagagcg tctggccttt
    25981 ttattccctg acgaggaaga acgtgttaag cgccgcccgg aatatgacga acgtctggag
    26041 actgaacttc aggttatcaa ccagatgggc ttcccgggct acttcctcat cgttatggaa
    26101 tttatccagt ggtcgaaaga taacggcgta ccggtagggc caggccgtgg ctccggtgcg
    26161 ggttcactgg tggcctacgc gctgaaaatc accgacctcg atccgctgga atttgacctg
    26221 ctgttcgaac gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc
    26281 tgtatggaga aacgcgatca ggttatcgag cacgtagcgg acatgtacgg tcgtgatgcg
    26341 gtatcgcaga tcatcacctt cggtacaatg gcggcgaaag cggtgatccg cgacgtaggc
    26401 cgcgtgctgg ggcatccgta cggctttgtc gatcgtatct cgaaactgat cccgcccgat
    26461 ccggggatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga aatctacgaa
    26521 gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg ggtcacccgt
    26581 aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac cgattttgcg
    26641 ccgctttact gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt
    26701 gaatacgccg gactggtgaa gttcgacttc cttggtttgc gtacgctcac catcatcaac
    26761 tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc
    26821 gctgcgatcc cgctggatga taagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg
    26881 gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct gcaacctgac
    26941 tgcttcgaag atatgatcgc actggtggca ctgttccgcc ccggcccgtt gcagtcaggg
    27001 atggtggata actttatcga ccgtaaacat ggtcgcgaag agatttccta tccggacgta
    27061 cagtggcagc atgaaagcct gaaaccggta ctggagccaa cctacggcat catcctgtat
    27121 caggaacagg tcatgcagat tgcgcaggtg ctttctggtt ataccctcgg tggcgcggat
    27181 atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga tggctaagca gcgttctgta
    27241 tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa aatcttcgac
    27301 ctggtggaga aattcgcggg ttacggattt aacaaatcgc actctgcggc ctatgctttg
    27361 gtgtcatatc aaacgttatg gctgaaagcg cactatccgg cggagtttat ggcggcggta
    27421 atgaccgccg atatggacaa caccgagaag gtggtgggcc tggtggatga gtgctggcgg
    27481 atggggctga aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac
    27541 gacgacggcg aaatcgtgta tggtattggc gccatcaaag gggtaggtga aggtccgatt
    27601 gaggccatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc
    27661 gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg aaaaactgat catgtccggg
    27721 gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga actcgctggg tgatgcgtta
    27781 aaagcggcag atcaacacgc gaaagcggaa gctatcggtc aggccgatat gttcggcgtg
    27841 ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc gtggccggag
    27901 caggtggtgt tagatgggga acgtgaaacg ttaggtctgt accttacggg acaccctatc
    27961 aaccagtatt taaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac
    28021 ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg
    28081 gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg
    28141 gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga aaaagaccgc
    28201 atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc
    28261 gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc
    28321 tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa
    28381 gcccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg
    28441 cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc
    28501 cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg
    28561 agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct
    28621 ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat
    28681 cgtctgcgtg aaaaaagcgt agaactgaca cgtaaaatct tcgccgatct cggtgcatgg
    28741 cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg
    28801 gcatttgatg aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc
    28861 gtcggtggta tcgcccgtct tgacggtcgt ccggtgatga tcattggtca tcaaaaaggt
    28921 cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc
    28981 aaagcactgc gtctgatgca aatggctgaa cgctttaaga tgcctatcat cacctttatc
    29041 gacaccccgg gggcttatcc tggcgtgggc gcagaagagc gtggtcagtc tgaagccatt
    29101 gcacgcaacc tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac ggttatcggt
    29161 gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa
    29221 tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgcc
    29281 gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct gaaagagctg
    29341 aaactgatcg actccatcat cccggaaccg ctgggcggcg ctcaccgtaa cccggaagcg
    29401 atggcggtat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga tgtgttaagc
    29461 actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaattc
    29521 gcaaaagttc tgaaaaaggg tcacttcggt
//
LOCUS       DAJTVJ010000044        28797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000044 DAJTVJ010000000
VERSION     DAJTVJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.044"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO448_001884"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO448_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6337101.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO448_001885"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO448_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6337102.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO448_001886"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO448_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6337103.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO448_001887"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO448_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6337104.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO448_001888"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO448_001888"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6337105.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO448_001889"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO448_001889"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6337106.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO448_001890"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO448_001890"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6337107.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="OO448_001891"
     CDS             complement(4604..5326)
                     /locus_tag="OO448_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6337108.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO448_001892"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO448_001892"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6337109.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="OO448_001893"
     CDS             6526..6744
                     /locus_tag="OO448_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6337110.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO448_001894"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO448_001894"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6337111.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="OO448_001895"
     CDS             complement(7478..7678)
                     /locus_tag="OO448_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6337112.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO448_001896"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO448_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6337113.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO448_001897"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO448_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6337114.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO448_001898"
     CDS             10175..12088
                     /locus_tag="OO448_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6337115.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO448_001899"
     CDS             12088..13110
                     /locus_tag="OO448_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6337116.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO448_001900"
     CDS             13104..13322
                     /locus_tag="OO448_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6337117.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO448_001901"
     CDS             13376..14245
                     /locus_tag="OO448_001901"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6337118.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="OO448_001902"
     CDS             complement(14300..14704)
                     /locus_tag="OO448_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6337119.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="OO448_001903"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="OO448_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6337120.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="OO448_001904"
     CDS             15689..17779
                     /locus_tag="OO448_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6337121.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OO448_001905"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OO448_001905"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6337122.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OO448_001906"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OO448_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6337123.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="OO448_001907"
     CDS             complement(19747..20349)
                     /locus_tag="OO448_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6337124.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="OO448_001908"
     CDS             complement(20339..20506)
                     /locus_tag="OO448_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6337125.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OO448_001909"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OO448_001909"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6337126.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="OO448_001910"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="OO448_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6337127.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="OO448_001911"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="OO448_001911"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6337128.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="OO448_001912"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="OO448_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6337129.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="OO448_001913"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="OO448_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6337130.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="OO448_001914"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="OO448_001914"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6337131.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="OO448_001915"
     CDS             28334..28501
                     /locus_tag="OO448_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6337132.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6995 a         7710 c         7219 g         6873 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctat
//
LOCUS       DAJTVJ010000045        28709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000045 DAJTVJ010000000
VERSION     DAJTVJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.045"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(301..507)
                     /locus_tag="OO448_001916"
     CDS             complement(301..507)
                     /locus_tag="OO448_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6337133.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            820..909
                     /gene="kdpF"
                     /locus_tag="OO448_001917"
     CDS             820..909
                     /gene="kdpF"
                     /locus_tag="OO448_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6337134.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            909..2582
                     /gene="kdpA"
                     /locus_tag="OO448_001918"
     CDS             909..2582
                     /gene="kdpA"
                     /locus_tag="OO448_001918"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6337135.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2605..4653
                     /gene="kdpB"
                     /locus_tag="OO448_001919"
     CDS             2605..4653
                     /gene="kdpB"
                     /locus_tag="OO448_001919"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR6337136.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4662..5234
                     /gene="kdpC"
                     /locus_tag="OO448_001920"
     CDS             4662..5234
                     /gene="kdpC"
                     /locus_tag="OO448_001920"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6337137.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5227..7911
                     /gene="kdpD"
                     /locus_tag="OO448_001921"
     CDS             5227..7911
                     /gene="kdpD"
                     /locus_tag="OO448_001921"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR6337138.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7908..8585
                     /gene="kdpE"
                     /locus_tag="OO448_001922"
     CDS             7908..8585
                     /gene="kdpE"
                     /locus_tag="OO448_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6337139.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8860..8964
                     /locus_tag="OO448_001923"
     CDS             8860..8964
                     /locus_tag="OO448_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6337140.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9275..11473
                     /gene="speF"
                     /locus_tag="OO448_001924"
     CDS             9275..11473
                     /gene="speF"
                     /locus_tag="OO448_001924"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR6337141.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11470..12789
                     /gene="potE"
                     /locus_tag="OO448_001925"
     CDS             11470..12789
                     /gene="potE"
                     /locus_tag="OO448_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6337142.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OO448_001926"
     CDS             complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OO448_001926"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6337143.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OO448_001927"
     CDS             complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OO448_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6337144.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15241..16005
                     /gene="ybfF"
                     /locus_tag="OO448_001928"
     CDS             15241..16005
                     /gene="ybfF"
                     /locus_tag="OO448_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6337145.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16145..16438
                     /gene="ybfE"
                     /locus_tag="OO448_001929"
     CDS             16145..16438
                     /gene="ybfE"
                     /locus_tag="OO448_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6337146.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16578..17108
                     /gene="fldA"
                     /locus_tag="OO448_001930"
     CDS             16578..17108
                     /gene="fldA"
                     /locus_tag="OO448_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6337147.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17318..17404
                     /gene="uof"
                     /locus_tag="OO448_001931"
     CDS             17318..17404
                     /gene="uof"
                     /locus_tag="OO448_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6337148.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17397..17843
                     /gene="fur"
                     /locus_tag="OO448_001932"
     CDS             17397..17843
                     /gene="fur"
                     /locus_tag="OO448_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6337149.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17928..18254)
                     /locus_tag="OO448_001933"
     CDS             complement(17928..18254)
                     /locus_tag="OO448_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337150.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OO448_001934"
                     /pseudo
     CDS             complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OO448_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OO448_001935"
     CDS             complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OO448_001935"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6337151.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22211..24157)
                     /gene="nagE"
                     /locus_tag="OO448_001936"
     CDS             complement(22211..24157)
                     /gene="nagE"
                     /locus_tag="OO448_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6337152.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24490..25290
                     /gene="nagB"
                     /locus_tag="OO448_001937"
     CDS             24490..25290
                     /gene="nagB"
                     /locus_tag="OO448_001937"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6337153.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25350..26498
                     /gene="nagA"
                     /locus_tag="OO448_001938"
     CDS             25350..26498
                     /gene="nagA"
                     /locus_tag="OO448_001938"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6337154.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26507..27727
                     /gene="nagC"
                     /locus_tag="OO448_001939"
                     /gene_synonym="nagR"
     CDS             26507..27727
                     /gene="nagC"
                     /locus_tag="OO448_001939"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6337155.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27775..28527
                     /gene="nagD"
                     /locus_tag="OO448_001940"
     CDS             27775..28527
                     /gene="nagD"
                     /locus_tag="OO448_001940"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR6337156.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         6731 a         7314 c         7522 g         7142 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatt
       61 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
      121 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      181 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      241 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      301 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      361 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      421 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      481 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      541 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      601 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      661 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      721 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      781 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      841 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      901 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      961 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
     1021 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1081 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1141 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1201 cgtgggatct ggcgttgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1261 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1321 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1381 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1441 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1501 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1561 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1621 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1681 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1741 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1801 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1861 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1921 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1981 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     2041 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2101 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg
     2161 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2221 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2281 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2341 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2401 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2461 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2521 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2581 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2641 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2701 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2761 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2821 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2881 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2941 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     3001 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3061 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3121 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3181 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3241 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3301 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3361 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3421 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3481 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3541 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3601 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3661 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3721 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3781 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3841 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3901 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3961 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     4021 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4081 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4141 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4201 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4261 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4321 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4381 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4441 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4501 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4561 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4621 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4681 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4741 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4801 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4861 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4921 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4981 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     5041 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5101 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5161 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5221 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5281 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5341 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5401 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5461 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5521 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5581 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5641 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5701 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5761 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5821 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5881 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5941 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     6001 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6061 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6121 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6181 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6241 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6301 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6361 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6421 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6481 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6541 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6601 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6661 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6721 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6781 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6841 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6901 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6961 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     7021 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7081 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7141 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7201 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7261 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7321 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7381 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7441 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7501 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7561 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7621 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7681 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7741 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7801 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7861 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7921 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7981 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     8041 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8101 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8161 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8221 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8281 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8341 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8401 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8461 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8521 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8581 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8641 cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac gaaattacaa
     8701 catttaagta acaaaacagc atctaatatg gcgatattca taattaatga tttattatct
     8761 ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca tggatagaat
     8821 aactctacct tcaactgaca cagcaagagg taaaggtaaa tggaaaataa taaccgctta
     8881 atgcctcata taaggcggac aacccatatc atgaagtttg cccatcgtaa tagttttgac
     8941 tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg tacaggtctc
     9001 cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat actgctaacc
     9061 cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg acgagtatag
     9121 ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact gatttattta
     9181 tcagcggtgg agctttgctt tttttccggc gtgatcgatt tctcctttga gaaattgagg
     9241 acctgctatt acctgaaata aagagatgaa taaaatgtca gaattaaaaa ttgccgttag
     9301 ccgttcttgc ccggattgtt tttccactca tcgtgcatgc gtgaatattg acgaaagtaa
     9361 ttatattgac gttgccgcca ttattttatc agtcaatgat gttgaacgtg gaaaactcga
     9421 tgaaatagac gctactggct atggcattcc tgtttttatt gcaacggaaa atgaagaacg
     9481 tgtaccagca gaatatctgc cacgtatttc tggtgtcttc gagcattgcg aatcacggaa
     9541 agagttttat ggacgtcagt tagaaaccgc tgctagccat tatgaaacgc aactgcgccc
     9601 gcccttcttc cgcgcactgg tcgattatgt caatcagggc aatagcgctt ttgactgccc
     9661 gggtcatcag ggaggtgagt ttttccgtcg tcatccggca gggaatcagt tcgtggagta
     9721 cttcggagaa acgctgttcc gttccgactt atgcaacgca gacgtagcaa tgggcgatct
     9781 gctgattcac gaaggcgcgc catgcattgc acagcaacat gcggcaaaag tgtttaatgc
     9841 cgataaaaca tacttcgttc taaatggcac ttcatcttct aacaaagtgg ttttaaacgc
     9901 cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat aaccacaaat ctaaccacca
     9961 cggagcgttg ctacaggctg gtgcaacacc ggtttatctg gaaacggcac gtaacccgta
    10021 tggctttatc ggtggcattg atgcgcactg ttttgaagaa agttacctgc gtgagctgat
    10081 cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg ctgtgattca
    10141 gttaggcacc tacgacggta cgatttataa cgcccgccag gtggtggata aaattggtca
    10201 tctgtgtgac tacattctgt ttgactccgc atgggttggc tatgagcaat ttatcccgat
    10261 gatggctgac tgttcgccgc tgttgctgga tcttaatgag aacgatccgg gtattctggt
    10321 tacgcaatcc gtacataaac agcaggctgg tttttctcaa acttcacaaa tccataaaaa
    10381 agacagccac atcaaagggc aacagcgtta tgtaccgcac aaacgcatga acaacgcctt
    10441 tatgatgcac gcctccacca gcccgttcta tccgctgttt gccgcactgg atatcaacgc
    10501 caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg taaatggcat
    10561 taatgcccgc aaactgatcc tcgataactg tcagcatatt cgtccgttcg tacctgaact
    10621 ggtggatggt aaaccctggc agtcgtatga aacagcgcaa attgcggttg atctgcgctt
    10681 cttccagttt gtaccagggg aacactggca ttcttttgaa ggctatgcag agaatcaata
    10741 ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt attgatgcac gtaacggcga
    10801 atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg aaaatggcgt
    10861 agtgccggaa aaatgcgatc ttaactccat cctcttcctg ctgactccgg cagaagatat
    10921 ggccaaactt cagcaacttg ttgccctgct ggtacgcttc gaaaaactgc ttgagtccga
    10981 cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc gctacgccgg
    11041 ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc acaacgtgaa
    11101 acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca tgaatccgca
    11161 agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctgc cggatgccga
    11221 aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc
    11281 gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag aagggatcaa
    11341 cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac atgatggtcg
    11401 taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc tgttgaaagg
    11461 ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt aaccatactg
    11521 acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtt
    11581 gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg
    11641 gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg ttatgccgag
    11701 tatgctttcg gtaaatccgg taactttatg gcgaactata cctacggcgt ctcactgctg
    11761 attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg
    11821 agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg
    11881 gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt gtggggggtc
    11941 attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc gacgctttac
    12001 gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc ttccatcgct
    12061 atgacgctgt gggcttttct cggtctggag tctgcgtgtg cgaatactga tgtagtggaa
    12121 aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt
    12181 tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct ggcaaattca
    12241 acggcaccat ttggcctggc cttcgcacag atgttcacac cagaagtggg taaagtcatt
    12301 atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc
    12361 caggtgttta agtcttcatc tgatgaaggc tacttcccta aaattttctc ccgtgtaacc
    12421 aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag cgggttggcg
    12481 ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa cctggccgtg
    12541 gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc
    12601 gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg
    12661 tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg ttccatcgtg
    12721 accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact gaaaaataaa
    12781 cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgccctttt tttattaaat
    12841 gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct
    12901 tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg
    12961 acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgccg atagaagcac
    13021 cattgcccgg tgccgccgtc aggcgcgcgg tgatcgggtc gcctgccagg gtgctggcgc
    13081 tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct
    13141 gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg
    13201 ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacatgatg atgccgtctt
    13261 tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc
    13321 cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggga
    13381 cttctaccag cttgcggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca
    13441 gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga
    13501 tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac
    13561 ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcaat gccagcaggc
    13621 ccgccatcgc acactcggag gagcagtcca tacggatcgc gccgtcttta tcgaggtgca
    13681 taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact
    13741 cgccaattcg cttccagtat tcgataccgg aaccacccag cggatcaacg cccagcgtca
    13801 ggcccgtttt ctgaatcgcc gccatatcaa cgatatcggc cagaccttcc acaaactgct
    13861 gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg gagatacgct
    13921 tcacgccttt caggccatcg gccatcagtg cgttggcacg atcttccacc actttagtga
    13981 cgttggtatc agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg
    14041 ggttatggga gggtgtaatc acgataccgt ctgccagcgg gccacctttt ttattatgaa
    14101 ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc tgcacaatga
    14161 catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg
    14221 cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag
    14281 caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc
    14341 cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca
    14401 gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt
    14461 ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt
    14521 ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc
    14581 atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca
    14641 tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtaa
    14701 acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa
    14761 tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg
    14821 tcagaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc
    14881 gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt
    14941 gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc
    15001 ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta
    15061 atccagtaca gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca
    15121 gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc
    15181 ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc
    15241 atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt
    15301 cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta
    15361 aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagatccg
    15421 gtaatgaatt acccggcgat ggcgcaggat cttgttgata ctctggatgc acagcagatc
    15481 gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc acttacggca
    15541 ctggcccgcg atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac
    15601 gtacgccgtc atgatgagat tttcgcggct atcaacgcgg tcagtgaatc ggacgcacaa
    15661 actcgccagc aagcagcagc aataatgcac cagcatctta atgaagaagg ggtgattcag
    15721 tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat
    15781 cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt
    15841 atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa
    15901 tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa
    15961 ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa
    16021 acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta
    16081 ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa
    16141 gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg
    16201 accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg
    16261 caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc
    16321 ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc aatatgagcc gtagcgaact
    16381 gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca tcgtttaaat
    16441 ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg caagactgcc
    16501 tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc
    16561 aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata
    16621 ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg
    16681 acattgcaaa aagcagcaaa gaagatctgg aagcttatga cattctgctg ctgggcatcc
    16741 caacctggta ttatggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag
    16801 agattgattt caacggcaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg
    16861 ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa
    16921 cgatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggctg
    16981 atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg
    17041 aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctca
    17101 atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc
    17161 aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc
    17221 aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag
    17281 ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc
    17341 aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga
    17401 ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa
    17461 tcctagaagt tcttcaggaa ccggacaacc atcacgtcag tgcggaagat ttatacaaac
    17521 gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt
    17581 ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac
    17641 tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag gttatcgaat
    17701 ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc
    17761 tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg
    17821 aacatgcgca cgaaggcaaa taagccagcc tgaatgagaa aaagccaacc tgcgggttgg
    17881 cttttttatg caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
    17941 ctgcgaattt cctgccaaac tttatcacaa tcggctttca ccgcctgatc atttccggtt
    18001 tgcgttgcac gcagatactg ctgataatcc agtacgcgga cactttcctg gtcggcaaaa
    18061 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
    18121 tctggcgaac cttgtgcggt gttgatacag gcgctgtatg cctctttcag acgactgtct
    18181 tcctgtggcg cggttgattg cgcacaagct accagccccg atgccattat ggcgatgaga
    18241 atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg
    18301 gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa
    18361 gatgttgccg taaccaccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa
    18421 gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga ccgcatccag
    18481 gctgtatgca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg
    18541 ctaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg
    18601 aagattccag tttttcaggt catacatcgc accgaagaag accgcttttt cgccgttagc
    18661 gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg aagcgtaggt
    18721 tggagtcata cgttgcagga agtatccctg ctgaccgtca gccttaaccc aggtgccttc
    18781 gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc
    18841 ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact ttatcgcgcg taccatagaa
    18901 ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa
    18961 atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga agttattttt
    19021 gaagtcgtat ttcgccccaa gggagtgcag ataatcaact ttggtggttt tatcgttctg
    19081 ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc acatgtagga
    19141 gaaactcaac gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg ccccctgata
    19201 agtacctggc ataaagctcc agtgcggcgc taacagcgtt tgaccagttg gctgaatgta
    19261 acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat acaggcttat
    19321 accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat
    19381 ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa acgcagcaat
    19441 atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt
    19501 ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc
    19561 acgctgccag taatatatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg
    19621 agcctccggc attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag
    19681 cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct
    19741 gcaccaaagg tgaacagcga aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    19801 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    19861 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    19921 gcggttgaaa ggcttgcaga agaataaaag cgtgaaattc cagcaagatg aaagcggtta
    19981 cattttattt aattatgttt tgttatttta aatttatttt atggataaat tgcgaagcgg
    20041 aattaattcg cgaagaaaag cagaaaaaac gccgctgaac gcggcgtttt ggattgtttg
    20101 catatttctt gccggatgcg gcgtgaacgc cttaaccggc ctacaaaagc acgcaaactc
    20161 aatatattgc agagatcatg taggcctgat aagcgtagtg catcaggcaa tttagcgttt
    20221 gaaactgttt gtctgttcct tgccggatgc gacgtaaacg ccccatccgg catagcgaaa
    20281 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
    20341 ccggtttttc cgccgtagaa tggcggctat cgaggcagaa gtaaccttca cgctcaaact
    20401 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
    20461 ccagcgattc cgggttaatc accgacagga aatcatccgc agcaccaggg ttcggcacgc
    20521 tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    20581 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    20641 aagtacagaa gatggtggtg atattgcctt cggcatcttt ctccacgcgt tctgccttaa
    20701 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
    20761 tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc accgctaaac ggcacctggc
    20821 ggctacccat ttccggtttg ttcggatggt tcggcatggt aaccatttcg ccttcgccct
    20881 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcgtttt
    20941 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
    21001 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc agcagtgtaa ccacgacgac
    21061 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    21121 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    21181 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac
    21241 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagg gcatcgctga
    21301 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    21361 cagcaaattt aatgcggtac agcaccggat cgcgcatcac gataaacggt gaagccatgt
    21421 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    21481 acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggttgcg
    21541 tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc
    21601 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    21661 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gactcaacat
    21721 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    21781 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
    21841 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
    21901 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    21961 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    22021 tgttagttag cgaaaatcga aaatctcact gagattatgg ggatgattga tgaaaaaaaa
    22081 agcggcagag aagatctcca ccgcttttgc cagtcccgcg tcattgttgg atgcgcgtct
    22141 tatccaacca ggactgagct tttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
    22201 ataaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca acattgccct
    22261 gagctttaat gatcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca
    22321 tcgaacgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc
    22381 tcacctgcgc gccctcttcc accagacgtt taaagccttt accttccagc gctacggtgt
    22441 cgatacccat gtggacgaca atctccgcgc ctttttcggt ttctaggcag aacgcgtggt
    22501 tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg
    22561 gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca gaaacctgat
    22621 ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg
    22681 gtacagcctg cggttttgct acaggagcgg cagttgccgg agttgcttca gctgacgcag
    22741 ctgctaccgg gccacgggca acgactttct tcatcgcatc gccaatggat tctgctttcg
    22801 cgccaacaat cacctgaatg gtctgtttgt tcagttttac tacaccagaa gcacccagac
    22861 gtttacacat cgcatcgttg acgcgagctg agtcagccac ggtaaggcgc agacgggtaa
    22921 tacaggcgtc aatcgctttc aggttgtcag taccgccaac cgcagcaata tagttggttg
    22981 ccagttgagt cagaccttct tcagtattgc tgttagcctc ttcagttacg atctcgtctt
    23041 ctttatcttc acgacccggc gttttcaggt tgaacatgcg gataaccaaa ctgaacacca
    23101 cgaagtagat agcgaagaag acaacaccca tcaccagcag catccagacg ttctggctgg
    23161 cggccggcag gttatacatc aacgcgtagt cgatagcccc cgcagagaaa gagaagccag
    23221 catggatacc cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga
    23281 ggtacagcag cggagcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca
    23341 ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg
    23401 gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcatg atcgggaaga
    23461 agccggacat gaacatcccc gcggtgccgt caccagcgta gaaacggttg atgtcgccgt
    23521 ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca
    23581 gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac
    23641 ccagcgcgcc tgcagaaacg atccactcgc cgcctgcatg gatagcgtgc tgtactggcg
    23701 gccagacgta accaaaaatg gccgccagca ccaggcagaa aaagccggtg gcaatcggca
    23761 caaagcgttt gccgccgaag aagctcagga agtccggcag tttaatatcg gaccaacggt
    23821 tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt
    23881 ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg
    23941 ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg
    24001 cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg
    24061 gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac cgcgataggg agctgtaacg
    24121 ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct
    24181 atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttgtactgcc gatgttctgt
    24241 aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgt atcgcaaatt
    24301 aaacgcgtgc cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg
    24361 gctaaagcag aaaacttatt ttatcattca aaaaatcagg tcggattgac gcctgtctgc
    24421 gcaaatccag gttacgctta aagatgccta atccgccaac ggcttacatt ttacttattg
    24481 aggtgaataa tgagactgat ccccctaact accgctgaac aggtcggcaa atgggctgct
    24541 cgccatatcg tcaatcgtat caatgcgttc aaaccgactg ccgatcgtcc gtttgttctg
    24601 ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa
    24661 gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg
    24721 ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt
    24781 gatattccag cagaaaacat caaccttctc aacggcaacg ccccggatat cgacgccgaa
    24841 tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt
    24901 gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact
    24961 cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat
    25021 gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacattgct ggatgccgaa
    25081 gaagtgatga ttctggtgct gggtagccag aaagcactgg cactgcaggc tgcagttgaa
    25141 ggttgcgtga accacatgtg gaccatcagc tgtctgcaac tgcatccaaa agcgatcatg
    25201 gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa
    25261 ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggct ccttgctcag
    25321 ggcaatattt tttaaaatcg gggggcagaa tgtatgcatt aacccagggc cggatcttta
    25381 ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg
    25441 tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc
    25501 tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca
    25561 ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag
    25621 gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg
    25681 tgcgcgttat gcgcgagtac ctggcaagac atccgaatca ggcgttaggt ctgcatctgg
    25741 aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc
    25801 ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc
    25861 tggcaccgga aatggttcct gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg
    25921 tttctgccgg tcactccaac gcgacgttga aagaagcgaa agccggtttc cgcgcgggga
    25981 ttacctttgc cacccatctg tacaacgcga tgccgtatat taccggtcgt gaaccgggcc
    26041 tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattatcgct gatggcctgc
    26101 atgttgatta cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg
    26161 ttaccgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt gcgggtaaaa
    26221 caatatacta ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct
    26281 taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca ctggatgaag
    26341 tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg cgttgagaaa cgtctcggca
    26401 cactcgccgc aggtaaagta gccaacctga cctcattcac acctgatttt aaaatcacca
    26461 agaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg
    26521 acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg
    26581 cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa gccagcttgc
    26641 ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt
    26701 tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa
    26761 tttccacgca atcggcgtac ggcttggtcg tcacgacgcc accatcaccc tgtttgatct
    26821 cagcagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc agcaaacgct
    26881 ggaacatgcc ctgttgaatg ccattgctca gtttattgat agctaccagc gcaaactgcg
    26941 cgagctgatc gcgatttcgg tgatcctgcc agggcttgtt gacccggaca gcggcaaaat
    27001 tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagctc tggaagaacg
    27061 ttttaaagtg acctgtttcg ttggtcacga tatccgtagt ctggcgctgg cggagcacta
    27121 cttcggtgca agtcaggatt gcgaagactc cattctggtg cgtgtccatc gcggaaccgg
    27181 ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggtaacg tcggtgaaat
    27241 tggccatatt caggtcgaac cgctgggtga acgctgccac tgcggcaact ttggctgcct
    27301 ggaaactatc gccgccaacg ctgccattga acaacgggtg ttgaatctgt taaagcaggg
    27361 ctaccagagc cgcgtgccgc tggacgactg caccatcaaa actatctgca aagccgcgaa
    27421 caaaggcgat agtctggcgt cggaagtaat tgagtatgtc ggtcgtcatc tgggtaaaac
    27481 catcgccatt gctatcaact tatttaatcc gcaaaaaatt gttattgccg gtgaaatcac
    27541 cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc aggcgctgaa
    27601 ggcgtttcgt actaatctgc cggtggtacg ttctgagctg gatcaccgct cggcaatcgg
    27661 cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc atttgctgga
    27721 aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta gtccatgacc
    27781 attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt cgccgtaccg
    27841 ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt gttgctgacc
    27901 aactatcctt cgcagaccgg gcaagatctg gcgaaccgct ttgccactgc cggtgtcgat
    27961 gtgccggata gcgtgtttta tacctctgcg atggcgaccg ccgatttcct gcgtcgccag
    28021 gaaggcaaga aagcatatgt ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc
    28081 ggtttcacta ttaccgatgt gaaccctgat ttcgtgattg ttggcgaaac gcgttcctac
    28141 aactgggaca tgatgcataa agcggcctat ttcgtcgcta acggtgcacg ctttatcgcc
    28201 accaacccgg acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt gtgtgcaggg
    28261 attgagaaaa tctccgggcg caaaccgttc tatgttggta agcccagccc gtggatcatc
    28321 cgcgcagcat taaacaaaat gcaggcgcat tcggaagaaa cggtgattgt cggcgataac
    28381 ttgcgcaccg acattctggc gggcttccag gcaggtctgg aaacgattct ggtgctttct
    28441 ggtgtttcat cgctcgacga tatcgacagt atgccgttcc gcccaagctg gatttacccg
    28501 tcagtcgctg aaatcgacgt tatctgaata ccacccacgt ctgcggacgt ggttggctct
    28561 gatgacaaac gccaaactgc ctgatgcgct acgcttatca ggcctacgca gctcctgcaa
    28621 tatattgaat tttcatgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga
    28681 acaaatcgca ctttatcagc aatttggcc
//
LOCUS       DAJTVJ010000046        28680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000046 DAJTVJ010000000
VERSION     DAJTVJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28680
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.046"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1046)
                     /locus_tag="OO448_001941"
     CDS             complement(<1..1046)
                     /locus_tag="OO448_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6337157.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="OO448_001942"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="OO448_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="OO448_001943"
     CDS             complement(2433..3281)
                     /locus_tag="OO448_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6337158.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="OO448_001944"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="OO448_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6337159.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="OO448_001945"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="OO448_001945"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6337160.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO448_001946"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO448_001946"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6337161.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQCLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="OO448_001947"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="OO448_001947"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6337162.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="OO448_001948"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="OO448_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6337163.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="OO448_001949"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="OO448_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6337164.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="OO448_001950"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="OO448_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6337165.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="OO448_001951"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="OO448_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6337166.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO448_001952"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO448_001952"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6337167.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="OO448_001953"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="OO448_001953"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6337168.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="OO448_001954"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="OO448_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="OO448_001955"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="OO448_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="OO448_001956"
     CDS             22669..23652
                     /locus_tag="OO448_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6337169.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYISAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="OO448_001957"
     CDS             23675..25186
                     /locus_tag="OO448_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6337170.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="OO448_001958"
     CDS             25211..26209
                     /locus_tag="OO448_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6337171.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="OO448_001959"
     CDS             26242..27336
                     /locus_tag="OO448_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6337172.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="OO448_001960"
     CDS             27348..28220
                     /locus_tag="OO448_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6337173.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28680)
                     /locus_tag="OO448_001961"
     CDS             complement(28268..>28680)
                     /locus_tag="OO448_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6337174.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6820 a         7092 c         7789 g         6979 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagaca ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaagctct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagagacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatat ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacgta
//
LOCUS       DAJTVJ010000047        28652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000047 DAJTVJ010000000
VERSION     DAJTVJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28652
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.047"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..839)
                     /gene="melB"
                     /locus_tag="OO448_001962"
     CDS             complement(<1..839)
                     /gene="melB"
                     /locus_tag="OO448_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6337175.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANL"
     gene            complement(942..2297)
                     /gene="melA"
                     /locus_tag="OO448_001963"
     CDS             complement(942..2297)
                     /gene="melA"
                     /locus_tag="OO448_001963"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6337176.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2580..3488
                     /gene="melR"
                     /locus_tag="OO448_001964"
     CDS             2580..3488
                     /gene="melR"
                     /locus_tag="OO448_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6337177.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3687..5954
                     /gene="adiA"
                     /locus_tag="OO448_001965"
     CDS             3687..5954
                     /gene="adiA"
                     /locus_tag="OO448_001965"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6337178.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGASGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6279..7040
                     /gene="adiY"
                     /locus_tag="OO448_001966"
     CDS             6279..7040
                     /gene="adiY"
                     /locus_tag="OO448_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6337179.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7177..8514
                     /gene="adiC"
                     /locus_tag="OO448_001967"
     CDS             7177..8514
                     /gene="adiC"
                     /locus_tag="OO448_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6337180.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8618..10261
                     /gene="eptA"
                     /locus_tag="OO448_001968"
     CDS             8618..10261
                     /gene="eptA"
                     /locus_tag="OO448_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6337181.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10258..10926
                     /gene="basR"
                     /locus_tag="OO448_001969"
     CDS             10258..10926
                     /gene="basR"
                     /locus_tag="OO448_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6337182.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10927..12027
                     /gene="pmrB"
                     /locus_tag="OO448_001970"
     CDS             10927..12027
                     /gene="pmrB"
                     /locus_tag="OO448_001970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6337183.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12003..12092)
                     /gene="pmrR"
                     /locus_tag="OO448_001971"
     CDS             complement(12003..12092)
                     /gene="pmrR"
                     /locus_tag="OO448_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6337184.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12204..13706)
                     /gene="proP"
                     /locus_tag="OO448_001972"
     CDS             complement(12204..13706)
                     /gene="proP"
                     /locus_tag="OO448_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6337185.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13970..14836)
                     /locus_tag="OO448_001973"
     CDS             complement(13970..14836)
                     /locus_tag="OO448_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6337186.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14833..17061)
                     /gene="crfC"
                     /locus_tag="OO448_001974"
     CDS             complement(14833..17061)
                     /gene="crfC"
                     /locus_tag="OO448_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6337187.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17474..17809
                     /locus_tag="OO448_001975"
     CDS             17474..17809
                     /locus_tag="OO448_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6337188.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17967..18410
                     /gene="yjdN"
                     /locus_tag="OO448_001976"
                     /pseudo
     CDS             17967..18410
                     /gene="yjdN"
                     /locus_tag="OO448_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18543..19331
                     /gene="phnC"
                     /locus_tag="OO448_001977"
     CDS             18543..19331
                     /gene="phnC"
                     /locus_tag="OO448_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6337189.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19356..20372
                     /gene="phnD"
                     /locus_tag="OO448_001978"
     CDS             19356..20372
                     /gene="phnD"
                     /locus_tag="OO448_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6337190.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20498..21277
                     /gene="phnE"
                     /locus_tag="OO448_001979"
     CDS             20498..21277
                     /gene="phnE"
                     /locus_tag="OO448_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6337191.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21298..22023
                     /gene="phnF"
                     /locus_tag="OO448_001980"
     CDS             21298..22023
                     /gene="phnF"
                     /locus_tag="OO448_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6337192.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22024..22476
                     /gene="phnG"
                     /locus_tag="OO448_001981"
     CDS             22024..22476
                     /gene="phnG"
                     /locus_tag="OO448_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6337193.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22473..23057
                     /gene="phnH"
                     /locus_tag="OO448_001982"
     CDS             22473..23057
                     /gene="phnH"
                     /locus_tag="OO448_001982"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6337194.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23057..24121
                     /gene="phnI"
                     /locus_tag="OO448_001983"
     CDS             23057..24121
                     /gene="phnI"
                     /locus_tag="OO448_001983"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6337195.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24114..24959
                     /gene="phnJ"
                     /locus_tag="OO448_001984"
     CDS             24114..24959
                     /gene="phnJ"
                     /locus_tag="OO448_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6337196.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24956..25714
                     /gene="phnK"
                     /locus_tag="OO448_001985"
     CDS             24956..25714
                     /gene="phnK"
                     /locus_tag="OO448_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6337197.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25825..26505
                     /gene="phnL"
                     /locus_tag="OO448_001986"
     CDS             25825..26505
                     /gene="phnL"
                     /locus_tag="OO448_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6337198.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26502..27638
                     /gene="phnM"
                     /locus_tag="OO448_001987"
     CDS             26502..27638
                     /gene="phnM"
                     /locus_tag="OO448_001987"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6337199.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27638..28195
                     /gene="phnN"
                     /locus_tag="OO448_001988"
     CDS             27638..28195
                     /gene="phnN"
                     /locus_tag="OO448_001988"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6337200.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28182..28616
                     /gene="phnO"
                     /locus_tag="OO448_001989"
     CDS             28182..28616
                     /gene="phnO"
                     /locus_tag="OO448_001989"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6337201.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
BASE COUNT         6933 a         7952 c         7398 g         6369 t
ORIGIN      
        1 aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc accgataaca
       61 tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt ataagcaaga
      121 gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac gatggcttta
      181 agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac ttcatgcaca
      241 ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag tgtgaacatc
      301 tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag cgtcacacct
      361 gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag ttgttcacgc
      421 tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc cataatggtg
      481 taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt agtaccttca
      541 aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt accgatcagg
      601 atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca tcccataatc
      661 ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac caaacccaca
      721 gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat gccgatcgca
      781 aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga aatgctcata
      841 gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc agaggtgtgc
      901 gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg caaccagcct
      961 ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat ttcgtcaatg
     1021 cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg gtcgcgattt
     1081 tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg catcagggcg
     1141 gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc atcaaccaga
     1201 caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc gttacggacg
     1261 ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt gctggcatat
     1321 tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag ctctttatgc
     1381 cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac tttataacgc
     1441 tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc aaaatgttct
     1501 gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac aatattctgg
     1561 cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata agccgccagc
     1621 agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag ttccaggtaa
     1681 aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc gatattgagg
     1741 tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac ctgtttgata
     1801 tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac gtagttgagc
     1861 atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag atgcggaatg
     1921 gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt ttgttccaga
     1981 ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc gccaatctga
     2041 aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt gtggcaggtg
     2101 attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg cgactcttcc
     2161 aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc ctcgcgatgg
     2221 aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat aaatgtaatt
     2281 ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc atggcaggcg
     2341 aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag atcctgcgtg
     2401 aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag ttttcggcct
     2461 catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg tgctcccact
     2521 cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga gagccatcca
     2581 tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc ccgctgtcgc
     2641 tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc atgcccacca
     2701 gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg gaatacctga
     2761 tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg gcctgtacac
     2821 cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat ctgccgatgc
     2881 atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact cacggcatgg
     2941 tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc tggcagcagg
     3001 aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt ggcctgatgc
     3061 tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc tcgcgcacac
     3121 acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg ggctttattg
     3181 ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc aaacttaacg
     3241 ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa cagtacatta
     3301 ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa agtattctcg
     3361 atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg ttcggcaaat
     3421 atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc cagacgtttc
     3481 ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga taataagcag
     3541 tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata tttcaaaaat
     3601 gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc cgcaacgaag
     3661 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
     3721 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
     3781 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
     3841 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
     3901 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
     3961 gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt gtcgatgaat
     4021 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt gccgcgatga
     4081 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagcgaca
     4141 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
     4201 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gatatgggca
     4261 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
     4321 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtgcttccg
     4381 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
     4441 gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa ccggtctaca
     4501 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
     4561 ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa gccgggcaaa
     4621 aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat aacgctaaag
     4681 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     4741 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     4801 atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg aatgcgcttt
     4861 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     4921 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     4981 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag gaagtgatcg
     5041 acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc accgctgacg
     5101 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     5161 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     5221 tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     5281 acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     5341 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     5401 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     5461 gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     5521 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     5581 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     5641 acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     5701 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     5761 actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     5821 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     5881 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     5941 gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     6001 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     6061 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     6121 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     6181 atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc tttgctttag
     6241 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     6301 cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg aaagaaccta
     6361 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     6421 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gattacctcc
     6481 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     6541 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     6601 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     6661 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     6721 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     6781 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa agcgaaaaca
     6841 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     6901 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     6961 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     7021 gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     7081 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     7141 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     7201 aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg gtcaggtgtt
     7261 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     7321 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgatcca
     7381 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     7441 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt ggtcattggc
     7501 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     7561 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     7621 cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     7681 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     7741 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     7801 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     7861 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     7921 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     7981 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     8041 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     8101 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     8161 atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa cgcaaccaaa
     8221 gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata tctttacacc
     8281 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     8341 gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc cggggcgaaa
     8401 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     8461 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     8521 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     8581 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     8641 cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     8701 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     8761 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     8821 aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg ttggcgcggc
     8881 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     8941 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     9001 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     9061 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     9121 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     9181 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     9241 tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc gcaacccgtt
     9301 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     9361 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     9421 cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt cagtaccgtg
     9481 catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc accaggaagg
     9541 cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca acgatggcgg
     9601 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctgg
     9661 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     9721 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     9781 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     9841 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     9901 cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata aatttaccac
     9961 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
    10021 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc tgctgtggct
    10081 gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
    10141 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
    10201 gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag tgagtgaatg
    10261 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
    10321 accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca aagccttgag
    10381 gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
    10441 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
    10501 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
    10561 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
    10621 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
    10681 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
    10741 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
    10801 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
    10861 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
    10921 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
    10981 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcatcttctg gctatggcat
    11041 gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg caacaacgat
    11101 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
    11161 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
    11221 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
    11281 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
    11341 ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca cgaactgcga
    11401 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
    11461 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
    11521 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
    11581 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
    11641 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
    11701 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg cagcaacatt
    11761 atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga aggaccgggt
    11821 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
    11881 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
    11941 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
    12001 gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac actgaacacg
    12061 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
    12121 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
    12181 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
    12241 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt ctgctcgata
    12301 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
    12361 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
    12421 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
    12481 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
    12541 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
    12601 gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
    12661 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac actaccaagt
    12721 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
    12781 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
    12841 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
    12901 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
    12961 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
    13021 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
    13081 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
    13141 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
    13201 tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc aacaaatatc
    13261 gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca gatcaacagc
    13321 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
    13381 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
    13441 aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
    13501 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
    13561 taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc cagtgatgct
    13621 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    13681 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca tttccgcaaa
    13741 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    13801 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    13861 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    13921 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    13981 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    14041 gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg ggctgtttat
    14101 ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct cccgaagagg
    14161 caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc tctgccagat
    14221 gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc gggcttccca
    14281 gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata aaacagcgcc
    14341 caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct
    14401 gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac
    14461 gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt
    14521 gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca
    14581 gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg
    14641 gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct
    14701 gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca tcatcgccct
    14761 gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg gggccgtcca
    14821 gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag
    14881 ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt gctgctgcat
    14941 cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta aacgcgtctg
    15001 taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg ccgtaacaga
    15061 ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat cgacttctgc
    15121 gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt tctcatttcc
    15181 ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg
    15241 cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca gctggcgagt
    15301 gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga accccaggac gaaaccctga
    15361 ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg ttaacacccg
    15421 gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg
    15481 cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct ccgaatcatc
    15541 ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca gaaagtggtt
    15601 ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt
    15661 gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca
    15721 agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt
    15781 caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt aagcggcatg
    15841 aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg
    15901 acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg
    15961 ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa
    16021 ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa atatctgctg
    16081 tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc
    16141 gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg gcaccgattg
    16201 ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata
    16261 atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag
    16321 atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata
    16381 actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc
    16441 ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt
    16501 caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac
    16561 gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt
    16621 cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat
    16681 tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag
    16741 cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc
    16801 attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca ccatttccag
    16861 tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga tattaggctg
    16921 actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc
    16981 cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc
    17041 atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt
    17101 tagccagatt gtttttgagt tctgtttgag ttctgttttt ggcttttata attactgcaa
    17161 gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc ttctgatgcg
    17221 tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa gtagagcatt
    17281 aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc gtttcctttc
    17341 aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag ggattttgtg
    17401 tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc tgctgacttt
    17461 tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat acacttacga
    17521 agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg cagaacctgc
    17581 acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg ctgacggcga
    17641 cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg
    17701 caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt gcaaaatcga
    17761 cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat tgtattgtga
    17821 tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact gcctgatgcg
    17881 acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact acacttaatt
    17941 ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct tttgccggta
    18001 actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg ctctataaaa
    18061 tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc ccttccggaa
    18121 tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg cattgccgga agcgacatca
    18181 tgatgagcga tgccatgccg tcaggaaaag ccagctactc cggctttacg ctggtgctcg
    18241 attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct aacggaaaaa
    18301 tcgaaatggc ctggcaggaa actttctagg cgcatggctt tggcaaagtc accgataaat
    18361 ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa cccgccggga
    18421 ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt agtcactttt
    18481 aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag ggaggctgca
    18541 tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag catcaggcgc
    18601 tgcatgcggt tgctctgaac attcatcacg gtgaaatggt ggctctgctt gggccgtcgg
    18661 gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc gataaatccg
    18721 ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt ctggcgcgcg
    18781 atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac ctggtgaacc
    18841 gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg ttctggcgca
    18901 cctgttttag ctggtttacc cgcgagcaga aacaacgcgc gttacaggcg ctgacccgcg
    18961 ttggcatggt gcattttgcc catcaacgcg tttccaccct ctccggcgga cagcagcagc
    19021 gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc gatgaaccca
    19081 tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc gacatcaatc
    19141 agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact
    19201 gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc agccaacagt
    19261 ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa gagaacgcga
    19321 aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata attgcctcgc
    19381 tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac gccgaagagc
    19441 aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac ctgaaaccgc
    19501 aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg aacgccttct
    19561 ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg gatatcgcct
    19621 ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag gtcttcgccc
    19681 agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc aacaaagata
    19741 gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc ttcggcaatg
    19801 gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt gccaaaaaca
    19861 atatctccgc cagcgacttt aagcgcaccg tcaacgccgg gcatgaaacc aacgcgctgg
    19921 ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc gacaagctga
    19981 aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg ccgctgatcc
    20041 caggcgatcc gatcgtctgg cgcaagaatc tctccgaaac caccaaagac aagatctacg
    20101 acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa cgcctgggct
    20161 gggcaccgtt tcgcgcctcc agcgacctgc aactggtgcc gattcgccag ctcgcactgt
    20221 ttaaagagat tcagggcgta aaaagcaata acggactgaa tgagcaggac aagctggcga
    20281 aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac gcgttaagcg
    20341 cgatgagttc ggtgagtaaa gcggtgcagt aatgtgctcg atcggtcccc tctcccttga
    20401 gggagagggt tagggtgagg gggaaatgcg cacgctgatg ccctcacccc ggccctctcc
    20461 cacagggaga gggagaaaac attaaacgga gccaaacatg caaaccatca ccatcgcccc
    20521 acccaagcgc agctggttct cgcttctgag ctgggccatt gttctcgccg tgttggtcgt
    20581 ctcgtggcag ggcgcggaaa tggccccgct tacgctgatc aaagacggcg gcaacatggc
    20641 gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt acctcaccga
    20701 aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcagtgg ttctctccat
    20761 ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc agcccgttcg
    20821 ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc tgttcgtggt
    20881 cgccgtcggt ctcggaccgt tcgctggcgt gctggcgcta tttattcaca ccaccggcgt
    20941 gctctcgaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg tggaaggcat
    21001 tcgcgccacc ggcgccaaca agctggaaga gatcctttac ggcgtgctgc cacaggtaat
    21061 gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct cggcgaccgt
    21121 cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga ttcgcggttt
    21181 ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca gcctgctgga
    21241 tttcctctct caacggttgc gtaagcactt tatctgataa gtgaggcatt gatatctatg
    21301 cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc cgcaaaactt
    21361 gagcaggagc ttcgtcaaca ctatcgctgc ggcgactatc ttcccgccga gcagcaactg
    21421 gcggcgcgct ttgaggtaaa ccgccacacc ctgcgccgcg ccatcgacca actggtggaa
    21481 aaaggctggg tacagcgccg tcagggcatc ggcgtgctgg tgctgatgcg cccgttcgat
    21541 tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg cagccatccc
    21601 accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgttgc tgacgcgctg
    21661 gggattaccg agggtgagaa cgttatccat ttgcgcaccc tgcgtcgcgt caacggcgtc
    21721 gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac actgcaacgt
    21781 ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc gctgcgccgc
    21841 agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg ccttgaaatc
    21901 ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg tgaaagcagc
    21961 ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac tatggagcac
    22021 tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc gcacagccaa
    22081 ccggctgaac tggcagcccg cctgaacgcg ctgaacatca ccgccgacta tgaagtgatc
    22141 cgcactgccg aaactggcct ggtgcagatt caggcgcgaa tgggcggtac cggcgaacgc
    22201 ttttttgccg gcgacgccac gctgacccgt gccgccgtac gcctgactga cggcaccctc
    22261 ggctacagct gggtgctggg gcgtgataaa cagcacgccg aacgctgcgc gctgattgat
    22321 gcgctgatgc agcaatctcg ccactttcaa aacttatcag aaacccttat tgccccgctg
    22381 gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc cagccgggtc
    22441 gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct tttatgcttc
    22501 ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc gagccgggcg
    22561 tgattgtcgc cctgtatcag ctcaaacgcg gctggcagcc gctgaatatc gccaccacca
    22621 gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccacg ccattaagta
    22681 acgatatcgt caatcagagc ctgcgttttc ataccaacgc gccgctggtc agccagccgg
    22741 aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctc aacgcccttt
    22801 ccaccgacac cgccgttgcg ccggaagcgg gcgcgacgct gattttacag gtcgccagcc
    22861 tgagcggcgg acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa cgaatgatcg
    22921 ctccgcagct gccagagtgc attctgcacg aactcaccga gcgcccgcac ccgttcccgc
    22981 tcggcatcga cctaatcctg acctgcggcg agcgcctgct ggctatcccg cgaaccactc
    23041 atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga tcgacgccgc
    23101 ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcccgaac tgagcgtcgc
    23161 ccagattgaa cagcagctta acctcgcggt ggatcgcgtg atgacagaag gcggcattgc
    23221 cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg tcgaagcgat
    23281 tttcctgcta cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg agccgctcga
    23341 caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata ttcccggcgg
    23401 ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca ctctgctggc
    23461 aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt cgccgcacgt
    23521 tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg gagcacagcc
    23581 ggatgacatc acccgcacgc cgccggttta cccctgttca cgttcttccc gtttgcagca
    23641 gttaatgcgc ggcgacgaag gctatttgct ggcgctggct tactccaccc agcgcggtta
    23701 cgggcgcaac cacccgttcg cgggcgagat ccgcagtggt tacatcgacg tgtcgattgt
    23761 gccggaagag ctgggatttg cggtaaacgt cggcgaactg ctgatgaccg agtgtgaaat
    23821 ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg gctacgggct
    23881 ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc gtgcgttgca
    23941 agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt tcgtgctggc
    24001 gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc cgcactacgt
    24061 cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga accatggcta
    24121 atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg atccgccgcg
    24181 ccatcttaaa agcggtggcg atccccggtt atcaggtacc gtttggcggg cgcgagatgc
    24241 cgatgccgta cggctgggga actggtggta tccagctcac cgccagcgtg attggcgaaa
    24301 gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc gtgtcgattc
    24361 gcaacttctt taagcgcgtg accggggtaa acaccactga atgtacggac gatgcgacgc
    24421 ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag atcattatct
    24481 tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg gagacccgca
    24541 ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa gatatcgccc
    24601 gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg cgctacgtga
    24661 tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg cccgccctgc
    24721 agctgttcgg cgcagggcgc gagaagcgca tctatgcggt gccgccgttt acccgcgtgg
    24781 aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag ccatgcgcca
    24841 tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc ggaaaccgca
    24901 tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa agccaatgaa
    24961 tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca aaggctttag
    25021 cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg aatccggctc
    25081 cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc agggggagat
    25141 tcactatgaa aaccgttccc tctatgcaat gagcgaggcc gaccgccgtc gcctgctgcg
    25201 caccgaatgg ggcgtggtgc atcagcatcc gctcgacggc ctgcgccgcc aggtgtcggc
    25261 aggcggcaat atcggcgagc ggctgatggc gaccggggcg cgacattacg gcgatattcg
    25321 cgctaccgcg cagaagtggc tggaagaggt ggagattccc gccaaccgta tcgacgacct
    25381 gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca acctggtgac
    25441 gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt cggtgcaggc
    25501 ccgcctgctc gacctgctgc gcggactggt ggtggagctg aacctcgcgg tagtgattgt
    25561 cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga tgaagcaggg
    25621 gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc atccgtatac
    25681 acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg gcgtaaacgc
    25741 cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcgtcag cgtcgcatcc
    25801 gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa aaccttcatc
    25861 ctgcaccagc aaaacggcgt gcgcctgccc gtcctcaacc gcgcctcgct catcgtcaac
    25921 gcgggcgaat gcgtggtgct ccacggccat tccggcagcg gaaaatcaac cctgctacgc
    25981 tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc agatcaaaca cggtgacgaa
    26041 tgggtcgatc tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa aaccaccgtt
    26101 ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt cggcgctgga agtggtgatg
    26161 cagccgctac tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc cgcgcgtctg
    26221 ctcacccgcc tgaacgtgcc ggaacgcctg tggcatctgg cgccatcgac cttctccggc
    26281 ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc cattctgctg
    26341 ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt cgagctgatt
    26401 cacgaagcca aagcccgtgg cgcggccatc gtcggcatct tccacgacga agccgtacgt
    26461 aatgacgttg ccgaccgcct gcacccaatg gaagcctctt catgattatc aataacgtca
    26521 aattggtgct ggaaaacgaa gtggtacacg gttcactgga ggtgcaggac ggcgaaatcc
    26581 gcgcctttgc cgaaagccag agccgcctgc cggaggcgat ggacggcgaa ggcggctggc
    26641 tgctgccggg gctgattgag ctgcataccg ataatctgga taaattcttc accccgcgcc
    26701 cgaaagttga ctggcctgcc cactcggcga tgagcagcca cgacgcgctg atggtggcga
    26761 gcggcatcac caccgtactg gatgccgtgg caattggcga cgtgcgcgac ggcggcgatc
    26821 ggctggtgaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa cgcggcgtca
    26881 accgcgccga gcaccgtctg catctgcgct gcgaactgcc gcatcacacc acgctgccgc
    26941 tgtttgaaaa actggtgcag cgcgagccgg tgacgctggt gtcgctgatg gaccactcgc
    27001 cgggccagcg ccagttcgcc aatcgcgaga agtatcgcga atattatcag ggcaaatact
    27061 ccctcactga tgcgcagatg cagcagtacg aagaagagca actggcgctc gccgcacgct
    27121 ggtcgcagcc gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgaaaa attgcgcttg
    27181 ccagccacga tgacgccacc cacgcccacg ttgctgaatc tcaccagctt ggcagcgtga
    27241 tcgccgaatt tcccaccacg ttcgaagcgg cggaagcctc gcgcaagcat ggcatgaacg
    27301 tgctgatggg cgcgccgaat attgtgcgcg gcggctcgca ctccggcaac gtggcggcca
    27361 gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac cccgccagcc
    27421 tgctcgatgc ggcatttcgc gtcgccgatg acgagagcaa ccgctttacg ctgccgcagg
    27481 cggtgaagct ggtgactaaa aatccagcgc aggcgcttaa tctccaggat cgcggggtga
    27541 ttggcgaggg caaacgcgcg gacttggtgc tggcgcatcg caagggcaat cacattcata
    27601 tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac tgatttggtt
    27661 aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc tgcgggaaca
    27721 aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgccg gaagcgaaaa
    27781 ccatatcgct ctgagcgagc aggagttttt tacccgcgcg gggcagaacc tactggcctt
    27841 aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagatcgacc tctggctgca
    27901 cgccggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg cgcgggcgcg
    27961 ctatcaatcg gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc tccgccagcg
    28021 cctggaaaac cgtggccgtg aaaacgccag tgaaattaac gcccgcctgg cgcgcgccgc
    28081 ccgctatact ccacaggatt gccatacgct caacaatgac ggcagcctgc gccagtcggt
    28141 cgacacgctg ctgacgctga ttcatcagaa ggagaaacac catgcctgct tgtgagcttc
    28201 gcccggccac gcagtatgat accgacgcgg tttacgcgct gatttgtgag ctaaaacagg
    28261 cggagtttga ccaccacgca tttcgcgtgg gctttagcgc caatctgcgc gacccgaaca
    28321 tgcgctatca tctggcgctg ctggatggcg aggttgtcgg catgatcggc ctgcatttgc
    28381 agtttcatct gcatcatgtc aactggatcg gcgaaattca ggaactggta gtgatgccgc
    28441 aggcgcgcgg cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa gaagcccgcc
    28501 aggccggggc cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac gcgcaccgtt
    28561 tctatctgcg agaaggctac gagcagagtc acttccgctt caccaaggcg ctgtaacatg
    28621 agtctgaccc tcacgctcac cggcaccggc gg
//
LOCUS       DAJTVJ010000048        28034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000048 DAJTVJ010000000
VERSION     DAJTVJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.048"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..124)
                     /locus_tag="OO448_001990"
     CDS             complement(<1..124)
                     /locus_tag="OO448_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6337202.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNE"
     gene            132..563
                     /gene="tnpA"
                     /locus_tag="OO448_001991"
                     /pseudo
     CDS             132..563
                     /gene="tnpA"
                     /locus_tag="OO448_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(694..2017)
                     /locus_tag="OO448_001992"
                     /pseudo
     CDS             complement(694..2017)
                     /locus_tag="OO448_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(2017..3633)
                     /locus_tag="OO448_001993"
     CDS             complement(2017..3633)
                     /locus_tag="OO448_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6337203.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3929..4645
                     /locus_tag="OO448_001994"
     CDS             3929..4645
                     /locus_tag="OO448_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6337204.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4642..6303)
                     /locus_tag="OO448_001995"
     CDS             complement(4642..6303)
                     /locus_tag="OO448_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6337205.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6499..6927)
                     /gene="ibpB"
                     /locus_tag="OO448_001996"
     CDS             complement(6499..6927)
                     /gene="ibpB"
                     /locus_tag="OO448_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6337206.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7039..7452)
                     /gene="ibpA"
                     /locus_tag="OO448_001997"
     CDS             complement(7039..7452)
                     /gene="ibpA"
                     /locus_tag="OO448_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6337207.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7446..7518)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7758..8090
                     /locus_tag="OO448_001998"
     CDS             7758..8090
                     /locus_tag="OO448_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6337208.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8092..9306)
                     /locus_tag="OO448_001999"
     CDS             complement(8092..9306)
                     /locus_tag="OO448_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6337209.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9407..10471
                     /gene="cbrA"
                     /locus_tag="OO448_002000"
     CDS             9407..10471
                     /gene="cbrA"
                     /locus_tag="OO448_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6337210.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10468..11805)
                     /locus_tag="OO448_002001"
     CDS             complement(10468..11805)
                     /locus_tag="OO448_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6337211.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11880..13028)
                     /gene="dgoD"
                     /locus_tag="OO448_002002"
                     /pseudo
     CDS             complement(11880..13028)
                     /gene="dgoD"
                     /locus_tag="OO448_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(13025..13642)
                     /gene="dgoA"
                     /locus_tag="OO448_002003"
     CDS             complement(13025..13642)
                     /gene="dgoA"
                     /locus_tag="OO448_002003"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6337212.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13626..14504)
                     /gene="dgoK"
                     /locus_tag="OO448_002004"
     CDS             complement(13626..14504)
                     /gene="dgoK"
                     /locus_tag="OO448_002004"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6337213.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14501..15190)
                     /gene="dgoR"
                     /locus_tag="OO448_002005"
     CDS             complement(14501..15190)
                     /gene="dgoR"
                     /locus_tag="OO448_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6337214.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15159..15791
                     /locus_tag="OO448_002006"
                     /pseudo
     CDS             15159..15791
                     /locus_tag="OO448_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15837..16649)
                     /gene="yidA"
                     /locus_tag="OO448_002007"
     CDS             complement(15837..16649)
                     /gene="yidA"
                     /locus_tag="OO448_002007"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6337215.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16764..17162)
                     /locus_tag="OO448_002008"
     CDS             complement(16764..17162)
                     /locus_tag="OO448_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6337216.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17402..19816)
                     /gene="gyrB"
                     /locus_tag="OO448_002009"
     CDS             complement(17402..19816)
                     /gene="gyrB"
                     /locus_tag="OO448_002009"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6337217.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19845..20918)
                     /gene="recF"
                     /locus_tag="OO448_002010"
     CDS             complement(19845..20918)
                     /gene="recF"
                     /locus_tag="OO448_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6337218.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20918..22018)
                     /gene="dnaN"
                     /locus_tag="OO448_002011"
     CDS             complement(20918..22018)
                     /gene="dnaN"
                     /locus_tag="OO448_002011"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6337219.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22023..23426)
                     /gene="dnaA"
                     /locus_tag="OO448_002012"
     CDS             complement(22023..23426)
                     /gene="dnaA"
                     /locus_tag="OO448_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6337220.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23723..23803
                     /locus_tag="OO448_002013"
     CDS             23723..23803
                     /locus_tag="OO448_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337221.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24033..24173
                     /gene="rpmH"
                     /locus_tag="OO448_002014"
     CDS             24033..24173
                     /gene="rpmH"
                     /locus_tag="OO448_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6337222.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24190..24549
                     /gene="rnpA"
                     /locus_tag="OO448_002015"
     CDS             24190..24549
                     /gene="rnpA"
                     /locus_tag="OO448_002015"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6337223.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24513..24770
                     /gene="yidD"
                     /locus_tag="OO448_002016"
     CDS             24513..24770
                     /gene="yidD"
                     /locus_tag="OO448_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6337224.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24773..26419
                     /gene="yidC"
                     /locus_tag="OO448_002017"
     CDS             24773..26419
                     /gene="yidC"
                     /locus_tag="OO448_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6337225.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26525..27889
                     /gene="mnmE"
                     /locus_tag="OO448_002018"
     CDS             26525..27889
                     /gene="mnmE"
                     /locus_tag="OO448_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6337226.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6747 a         7580 c         7146 g         6561 t
ORIGIN      
        1 tctcattctg acgtgccaga gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc
       61 tcatttcacg ctcctgttga ccaccgggtc ttaactggaa tttaaatgct tgtagtcgtt
      121 tcacaggcca aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc
      181 attgtgtttt cctgatgcat gttcacctgg tctttgtcac caaataccga cgtcagattt
      241 ttgactatga cgcgacagaa aaactacgca cttacttttc aaatgtatgt gctgattttg
      301 aagctgaact ggtttaaatg gatggcgaac cagatcacgt ccatttgtta attaactatc
      361 ctcccaaact ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg ggtaggttac
      421 tgcgacgaga tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtc
      481 ctggctattt tgccaatagc tgcggaggtg cgccaatatc cgtcatccgc caatacattg
      541 aacaacagca aacacctggt taggtggaaa accgcgcctt atatccccgc cctgaaggac
      601 ggggttttac ggcgcaccgg ataaatttgg ttatgcgccg tcagccgtgc taaagaatac
      661 ggaccagcac ccaacggatg ccagtccaca caattaacgt aactctggcc agtacgcttt
      721 gttggcctcc agcaactcat ccagaatagc tttggcgacc gaggcgctcg gtaccgtttt
      781 cgataacgta atcgcctgcc acaggtgctg atatgaacgc tgttcccagg cgtccacgac
      841 cagtttttcc accgccactt gctgactcat taaccctttc tgaaactgcg ggatatcgcc
      901 gaccaccagt ggttctggtc cgttgtgccc gaccagacac gggatctcga ccatcgcttc
      961 atcatcaaag ttatggattg ccccgttgtt aggcacaatc agcaacatcc gctcctgagt
     1021 gttgaaggca attgccgccg ccagatcgac gatgtatgac gcatgttcgt caatctccag
     1081 cttggccgcc gctgaatttc ctgccgctgt aatggcgcgg caagcatcga aaacctgttt
     1141 ctcgcgatgt tccattcacc tcattcgcgc gggtatgttc agggttggaa tgctgaacaa
     1201 cataatccgg gaagagataa tatttcagat aggtgttcgg caatgtatcc gggtccagtg
     1261 cctggacatc ccgcgctttt gcataggtgt cgttccagct cgcttcagta tgtgtatctt
     1321 gctgcggtgg aacataaccg tgcttcgaga catattgccg cagctggggc atcaggtcgt
     1381 tgccctgcaa atcttcaatt gatgtccacc agccaaagtg gttcaggccg tagtagcgca
     1441 cgcgcatctg tttgcgatct tgcagcccaa caatttgcgc catccggctt tcaataccga
     1501 ttggcatgtc acagatgttg aggattttcg cattcgggcg cagacgacgc gtggcttctg
     1561 cgacaatggc tgccgggttg gagtagttga gcatccaggc atttggtgaa tatttttcca
     1621 tataatccac cagttccagg acgccgccaa tggaacgcat gccgtacgct attccgccgg
     1681 gtccgcaagt ttcctgacca acaacaccgt ggcgcagtgg gattttttca tccagttcgc
     1741 gcatcgggta cttgccgacg cggatgtgcg ccatgacaaa atcaacgtcg ctgaacgcca
     1801 cttcaggatc ggtggtgtaa ctaaacgcaa tgtccggcgc tttctcttta aggatgactt
     1861 tacaggcttc ggcaatcact tcctgccgcg cgccatcgtt atcataaaat ttcagtgcac
     1921 gaagcgggaa acgctcctga ttcgccagga gcatcaacac gatgcctggc gtaaaggtgc
     1981 ttccaccgcc tgcgacaacc actgagaatt tggtcattat actgcctccg taatggcaac
     2041 attttctgct gattgatgag aattaattaa gctatcgagc tgttcacgca gctgggatac
     2101 atgcagacca atgatcacct gaatggcatc gccactacgg aagacgccgt gcgctcccag
     2161 ctttttaaag acttcgtcat ccagcgtttg tgacatgtca tgcagtgcaa tgcgtagacg
     2221 cgtcgcacaa ttgttaatgc tggagatatt gccgaccccg cccagggctt gcaggatacc
     2281 ggcagcctga tccagctctt tttttggctc tgccgcggtg gtttggcctc gcgaggcttt
     2341 gtattcggct tttgagtaga gtttcacttc cgcatcttca cgtcccggcg tgcacatgtt
     2401 gaactgcaga atcagggtgc ggaaaaccac gaagtacagc agggtaaagc acaacccaat
     2461 ggcgatttgg gtcagcatca tatccgcgtg gttgctgaac atcgggatcc agttttgcgg
     2521 taaaacctgg tcaatcagac ctccgcccat gttgcccacc acaccaaaga gatacatcac
     2581 ggtcgacatt gaggccgcaa gcacagcgtg taccgcaaac agcaacggtg aaatgaacag
     2641 gaaggtaaat tccagcggtt cggtaattcc caccagcatg gcggttaagg ttgcgggaat
     2701 cagcaagccc gccactttta cccgattttc cggtgcggca gtgaagtaca tcgctaaaga
     2761 aatgcccacg gcaccaaaga tttttgagtt accgtgcagg gcaaaaccgc cttccgggaa
     2821 caacgatttc agcggctcgg cgctcaaact gaactcttgc agatgctgcg cccagtacat
     2881 ctgaatgccg ccttcaacag ctgccggacc caagataaac ggtccgtaga tgaagtggtg
     2941 taaaccggtt gggatcagaa tacgttcgag gaaggtgtaa acccagaccc caagcgcacc
     3001 cgccgaacgc aggaacgctt gcagagattc aatccccgtt tgtacttttg gccagccgag
     3061 caatgtcagc caggcacagg ggatcatcac caggaaggcg ataatcacca cataagacgt
     3121 cccctggaaa atgccgagaa aaaccggcag ttttttatcg aacagacggt tatgcagcgc
     3181 cgtcacaatg ccggaaatga taattgcgcc gataatgctg gtatcgaggg ttttaatccc
     3241 ggccatcatt gtcagaccgc tacctgccac cgcgtcctga gtgaaatcga cgccgaagta
     3301 gcttccccag gtcattccca tcgcgttgat gaaatagttc caggtcagga aactcaccat
     3361 caccgccaga caagcacgtc cctgcgcttg cttagcaagg ccaatgggta aaccgacagc
     3421 aaaaatcagc ggcatattac ggaataccgt ccaaccgccc tcttcaataa tgtgtacgat
     3481 ttgcgcgaat aaactgttcg gatcggtcag tgattccccg acaaacatcg ggttttgcag
     3541 caagatggca agacccacca caatcccggc gaagggaaac agcagcactg gcgtaaacat
     3601 cgcgccgcca aagcgttgaa tttgactgag catttccaca tcctcttttc tcaattctgt
     3661 aagtaggaag cctgattggt tatgcggcag gcctggagca aggataggaa gatgcgatcg
     3721 gcagaacatg acgacccaat gggattcgtg atcgctttca tgcttttcgt tgggcggtag
     3781 agatctacgt tttcgcgaaa aagtagacat ctgtgagcac aggtacacag catcgaacac
     3841 atctttaaaa aaaagatgtt ttttcaatcg attaagcaga acttgtgtgc gcattacccg
     3901 ggcttgcagg caaaaaagag atctagagat gatctacaaa agcattgcgg agcggttaag
     3961 aattcgactt aactccgcag atttcacgct aaacagcctt cttccgggtg aaaaaaagct
     4021 ggcggaagag tttgcggtat cgcggatgac catccgtaaa gccattgacc tgctggtagc
     4081 gtgggggctg gtggtccgcc gccacggtag tggcacttac ctggtgcgca aagatgtact
     4141 gcatcaaacc gccagtctga ccgggctggt ggaggtgtta aaacggcagg gaaaaactgt
     4201 caccagccag gtgctgattt ttgaaatcat gcctgcgcct ccggccattg ccagccagtt
     4261 acggattcaa atcaacgagc agatctactt ctcccgtcgc gttcgttttg tggaagggaa
     4321 accgctgatg ctggaagaca gctatatgcc ggtaaaactg ttccgtaatc tttcgctgca
     4381 acatctggaa gggtcgaagt ttgaatatat tgaacaagag tgcgggattt tgattggcgg
     4441 taattatgaa agcctgacgc cggtgctcgc cgatagactg ctggcgcggc aaatgaaggt
     4501 agcggaacac acgccactgc tgcggatcac ctcgttgtca tatagcgaga gcggggagtt
     4561 tttgaattat tcagtgatgt tcagaaatgc cagcgaatac caggtggagt accatttacg
     4621 gcgactccac ccggaaaaga gttaaccgat actccagaag agcaccgcca gtaattgggg
     4681 ggtgataatg cgcaggaaca tcactaacgg atagacagtg gcgtaagaga gcgccgccgc
     4741 accgctagtt ggatgaagat tattagcaaa cgccagcgcc ggaggatcgg tcatggaacc
     4801 tgccagcatc ccgcacatgg tcaggtaatt cattttggct aacatccgcg ccagaatgcc
     4861 aacagtaatc agcggaacgg cggtgatcag ggcaccataa ccaatccagc ttagcccttc
     4921 gccattgacc agggtattca caaaatcccc accagatttc agaccaacga ccgagaggaa
     4981 cagcacgatc cccagctccc gcagcgcgag gttggcactt ggcggcataa accagtacag
     5041 cttgccgata ctgccgatac gcccgaggat caacgccata atcagcagac cgcccgccag
     5101 ccccagtttc aacgcggccg ggaatcccgg cacaaagacg ggaatagaac ctaacaatac
     5161 gccaagcccg atgccaataa acactggcag catctgaacc tgttgcagtt tttgctgcgc
     5221 attccccagc acattggcaa cggcatcaat tgcggacgga cgccccacca ggttgaggat
     5281 atcgccgaac tgcaggctga tatcgccact ggcgaccagt tcgaccccgg cacggttcag
     5341 gcgcgagata acaacgtcat agcgttcttt aaagtgcagg tcgcgaatac gttttccgag
     5401 cacgttttca ttggtgacca ccacacgctc gacgcgcaaa tcagtgtctt tcgtggacag
     5461 cgaagtatcg acctcctgac caatcaccag ttgcgcatta tgtaaatccg ctggctgacc
     5521 caccagatgc agcaaatcgc ccagttggat aatggtatct ggcgaaggaa cttttagggt
     5581 ttcttcgcgt ttcagacgcg agcagataat tttgtcgccg ttgagaatcg gtacatcttt
     5641 aatggctaaa tcatgcaggt tagggttctc aacgcgaata ttgatagtct tgatcagcgc
     5701 gccgccattg gtgcgtgaag actcgtgctg ctgagcttct gtctcgacat tgacgcggaa
     5761 aataacccgc aacatccaca tggtgaacaa aatcccgcaa atgccgaatg gatacgccat
     5821 cgcgtaactc atccccatct gatcgaccat ttccattggt gtacccaggt cgcgcaaaat
     5881 ctgctgccct gcccccagcg ctggcgtatt ggtaaccgca ccggagaaaa tccccagcac
     5941 taccggcagt ggaatatcaa acagtttatg caggatggcg gtaaccagac caccgatgat
     6001 gacgatcaga acagcaaaca ggttgaggcg taatccggag acgcgcaatg aggcaaagaa
     6061 gcccggccct acctgaatcc cgatggtata aacgaacagg atcaggccaa attcctgaat
     6121 aacatgcagc atatcgctac ttaatgtcat ccccgcctga gaaacaaaat ggccgacgat
     6181 gatcccacca aacagcacgc cgccaatacc taatcctatg ccgcgaaatt tgacgttgcc
     6241 gataaacaaa ccgacgactg ccaccaaagc cagaatactg accgttaatg ctatatcact
     6301 catcgtccat ttctcttgag ataacatttt aattgctacg gattatggct gaatcggtga
     6361 agcagatttg ccttatagcg cacattatta cgggaaagaa cgtgccgaaa tatcttaaac
     6421 agtcgaaatt taacgcattg ttgaagaaga aagacaaaaa aagccccacc agaatggcgg
     6481 ggcaaagaga atagctagtt agctatttaa cgcgggacgt tcgctgatag cgatacgctg
     6541 cgctgcgatg ggttcaggct cattacgaat taaatcaata tgcagtaaac cgtttacgaa
     6601 agttgcgcca gagacttcca tattttcagc cagcgtaaag ctcaggctaa atggctgatt
     6661 cataagccct tgatgcagcc attttttctc ttcttttggc tgctccggcg tgccttttac
     6721 gctcaggcgc gtaccttcca gttgaatctc taaatcttcc tgacggaaac ctgccagcgc
     6781 aagggtaatg cggtagtggt tatcgtcgct tttctcaatg ttgtacggcg ggaagctctg
     6841 actttcaccg gcgttttgca gtgcgttggc cagtttgtca aaaccgatcc attgacgcat
     6901 cagtggggat aaatcgaagt tacgcatagt catttctcct tctaagaagc gagtaagtac
     6961 ctgcaaatcc gaagattcgc atatgctccc tgacggcgag catggagatg tcaggccgcg
     7021 ccaggcggcc ttaaggaatt agttgatttc gatacggcgc ggttttttcg cttccggaat
     7081 cacgcgttcg agatcgatat acagcaaacc atttaccagg ttagcgccac gaacatgaat
     7141 gttctcagct aactggaatt tgcgttcaaa gttgcgttca gcgatgccct ggtacagata
     7201 ggtgcgctct ttttgttcgt cggcgtgagc acctttcacc accagcagat tatcctgggc
     7261 ggtaatttcc agttcgctct cagcaaaacc agccacagcg atagcaatgc ggtaatggtt
     7321 ttcgtctacc agttcaacgt tatacggagg gtagccgcca ttactctggc tctggttgtt
     7381 ttctaagtgg ttaaacaaac ggtcaaatcc aatagcagaa cggtaaagcg gggataaatc
     7441 aaagttacgc ataatcaata gctcctgaaa tcagcgagaa tgtaagacct tccacaatgg
     7501 acaggtcagg tagccagaac acccatcagg cgcgtcctca tcgactacga tgtaaaaatg
     7561 ggtctggaaa tgaacttttc aagccttatc agcggacttt tttttgcagt ttatggtcta
     7621 ttgcatagac tgagggggca gcacgaaacg ttaaagtgtg atagccgcca cagagcgacg
     7681 taatgatgat gttattttca gcaagggatc gctataactc atcaggccaa cacccacgat
     7741 caacagatga atttattatg ataagaaatg tgttgttagc gttcatgata tgcagcggaa
     7801 tggcattact cggaggatgc tccagcgtga tgtcccatac cggagggaaa gaaggaacgt
     7861 atcccggcac ccgcgccagc gccaccatga ttggcgatga tgaaaccaac tggggcacta
     7921 agtcgctggc gattctcgat atgccgttta ccgctgtgat ggacacgctt ttattaccgt
     7981 gggatgtgtt ccgcaaagac agctcggtgc gctcacgcgt tgaaaaaagc gaagccaacg
     8041 cgcaagccac caatgcggtt attccaccgg ccagaatgcc tgataactga tttagcgatc
     8101 agtttcggtg atccacagct ggccaccttc catccacgcc agccattgac catccggcga
     8161 gaagaccacg gcgtccgcag aaggtggatt ggcgtggttt tcggttaaat actcaacctc
     8221 gccgctttgc gcatgggcac aagcaattcg attatccagc acaaagccca accattctcc
     8281 tgacggatgc cagttaaatg cagactgaat atccgttttg ttatgggtta actggcgcgg
     8341 ctcgccgccc tgtggcgaga taagccacag ttgcacaatg ccgttatcat cacgcattaa
     8401 aaacgcgatt tgcgtaccct gcggattaca gcgcacccag tggcgcggga cgttgactaa
     8461 ccccggataa gcccgatggt gggtaaaggt taaacgtcgc tgcacgacgc cacgcggtgg
     8521 cgcgggcagg gtggtttccg ttccacttaa cggcgcatca cctgccgctt tccagccagc
     8581 ttcatcttgc ggcaactcaa cgataaacag ctccggcact ttctcaccct ttggcgaaag
     8641 tgtgtcgcca ataaacgcca gcgcgtgatt tcctacccat ccttcttcat aagcacgatt
     8701 gatttcatca ctgccaggct gcggcgtggg cgtggtttta ctcaccagca cacaccagtg
     8761 gctgccgctg tattcacgcg gatgctgctt ttgtacgttg accggaccaa acggcgcagc
     8821 aacaccgacg tttcgcaaat ccagcgccgg atcgagttca tgcattacat ggtcgttata
     8881 ggtaaagctc accctttcac cgttcgggct aaagacatgc acatggctgc cgccgcgcag
     8941 cgctcctggc gtatacggcg cggtaatatc cattgcatcg agattgctca ccttgccgcc
     9001 ttcagcaatg actccgcgcc gatgatggaa gtcgtaatgc catgtttcat caggattttc
     9061 cgggccgtga ataaagacat atttctctga cttcggatga acggtcacca cgccgacgtg
     9121 tgcgccctgt gacgcacagt agataacctc gacctcaccg gtatggatat tcacacgctc
     9181 aatggtctcg ccggtaaacg acgccccaga aggacgcacg tcaaatacca gccactggct
     9241 gtcgggcgtc caggtattgg tattggtgag caggtgatta cggggagcaa aggttatctg
     9301 tttcatggcg cggtcctgat acaaaacggg tccagccatc atacttcaca aggacttcac
     9361 tttcagacaa tagtcttttg ccatcgtgat gaatgtgagt gaaaagatgg aacatttcga
     9421 cgtggcgatt attggcctcg gcccggcagg gtcggcgttg gcacgaaagt tagccggcaa
     9481 aatgcaggtg atcgcgctgg ataaaaagca ccagcatggt actgaaggtt tcagcaaacc
     9541 ttgtggcggt ctgctggcac cggacgcgca gcgatctttt attcgcgatg ggctgacgct
     9601 tcctgtcgat gtgatcgcta atccgcagat tttcagcgtc aaaaccgtcg acgtcgccgc
     9661 atcgctcacg cgtaactacc agcgaagcta tatcaatatt aatcgccatg ctttcgactt
     9721 gtggatgaaa tcgctgatcc ccgccagcgt tgaggtttat cacgacagcc tgtgtcgaaa
     9781 aatctggcgt gaggatgata aatggcatgt catttttcgt gcagacggct gggagcagca
     9841 tattaccgcc cgctatctgg tcggtgccga tggtgccaac tcgatggtgc ggcgacatct
     9901 ctacccggat catcaaatcc gtaaatatgt cgcaatccag cagtggttcg cagagaaaca
     9961 tccggtgccg ttctactcct gcatctttga taatgcgata actgactgtt attcatggag
    10021 tatcagcaaa gacggttatt ttatctttgg cggtgcctat ccaatgaaag acggtcagac
    10081 gcgtttcacg actctgaaag agaaaatgag cgcctttcag ttccagtttg gcaaagcggt
    10141 gaaaagcgaa aaatgcacgg tgctgtttcc ctcacgctgg caggattttg tctgcggtaa
    10201 ggacaacgcc tttctgattg gtgaagcggc gggatttatc agcgccagct cgctggaggg
    10261 gattagctat gcgctggata gcgcagacat tctgcgttcg gtgttactga agcagccaga
    10321 gaagctcaat accgcttact ggcgcgccac ccgcaaactg cgtttaaaac tcttcggcaa
    10381 gatagtaaaa agccgatgcc tgaccgcacc ggctttaaga aagtggatta tgcgcagtgg
    10441 tgtggcacat attccacagt tgaaggatta gccaacgcgc ttcacatcgc ccaccagcag
    10501 gatataagag agcgcgccaa tcaacgcgac ggcggagata taaaccagtg caggtgcgaa
    10561 accgtaaccc tgcgccaggt accccaccac cagcggaacg gtgatgccgc ccagaccacc
    10621 ggcgaagtta aacacgccgc cggttaaacc aatcaggcgc atcggtgcca gagaagaaac
    10681 cagcgaccag gtaattgaag caaaaccgtt accgaagaat gccagcgcca tcaggcacat
    10741 aatcatcatc ggatcgttag tgtagttagc gcccataatg caggtggaga tcagcaagcc
    10801 gcagataatc ggcgttttac gcgcaaagcc cagtgaaaag cccttacgta ccagcagatc
    10861 cgctacccag ccagagagca ggacgccgac aaacgccgcg aggaatggca ccgtggtcat
    10921 aaagcctgct ttcagcgccg tgattccttt ttcctgggtt aaatagttcg ggaaccaggt
    10981 taagaaaaac cacagtgtag aagccaccgc aaattgccca agatagacgc cgatcagttt
    11041 acgatggaac accagtttcc agtctttggc tgttaacggc tgacgtgcct ctttcttcac
    11101 tggcgcatca ccatccacca gaccgccgcc atcacgaatg taatccagtt cagctttgct
    11161 gatgcctttg gtcaggcgcg gcggctgata aaccttaaac caaatcagcg accagataat
    11221 gccgatacca ccagtcacaa tgaacaccca gtgccagctc aacatctcct gaatccagat
    11281 cagcagcgga gtcaaaaacg ccagaccgac aaactgacca gacgtataaa aaccaacggc
    11341 ggaggcgcgt tcatgttccg ggaaccagct ggtcaccatc cggttattgg tcgggaaagc
    11401 gggcgcttcg aaaataccgg ttatcgcacg cagaccaatt aatgacatta atcccgtggc
    11461 aaagccctgg aataaagtcg ccactgacca gccaaatatc gcaataaaat aagtcacgcg
    11521 agaacctacg cgatctaaaa accaaccgcc ggggatctgg catagcgtat aaagccaggc
    11581 gaaggccgaa aatacatagc ccatttccgc tttcgtaatg ccgaactctt cctgaatatg
    11641 ggcggaagcc acggccaggt tagcgcggtc aacataacaa atgaccaccg taataaagat
    11701 catcaccagc gtcagataac gccgacgccc cggctttgct gcattaacgg gaatatccat
    11761 agcgagcttt ctccagattt tgggcatagc gaagccgctc accatgccct gtaatttaca
    11821 gagggttata tttttgtatt gctgttttag tgcccgatga ggggcttacg tggcaggaat
    11881 taccactctg ctacgctgtt atcttcatga cgccagagtg gattacgcca gtccggggca
    11941 tttttactga actcaataac tttagcttcg tcgatttcca cgcctaagcc cggtttcgtt
    12001 agcggtttaa agaagccgcc aaccatgctg aagtcttctt tgtttttcac aaagtcgagt
    12061 aactccgcgc ctttgttgta atgaattccc atactttgtt cctgaagtac cgcgttatag
    12121 gaaacaaagt cgatatgcag acaagccgcc agtgcaatcg gtccgagcgg acagtgcggc
    12181 gcaagggtca cgtcataggc ttctgccatt ccggcaattt tgtagcattc ggtaataccg
    12241 cctgcatggg agagatccgg ttgtagaatc gaaataccgc ctgcctccag cacgcgttta
    12301 aaatcgaaac gtgagaacat gcgctcaccc gccgccagtg gaatatgcgt ttgtgccgcc
    12361 aatttcgggt agtattcggc ttgttccgcc agcaccggct cttcaataaa cagcgggcga
    12421 tacggctcca gttctttaat cagcactttc gccattggcg cgctgacgcg accgtggaaa
    12481 tcaagaccaa actcaatctg attgccaaaa gcttcacgaa tttgtgccac ggtgttgact
    12541 gccgcatcta ccgcgcggga gttatcaatt agccccagtt cttcacaacc gttcagtttg
    12601 aaggtatcga agccgatttc gcgcagggtt ttaataccgt cgatcacatc cgccggacga
    12661 tcgccgccga cccaactgta ggctttaatt ttgtcgcgaa ccaggccgcc catcagttgc
    12721 cagaccgacg cattcagcac tttacctttg atatcccata acgcctggtc gatcccggca
    12781 atggcgctca tcaggattgg accgccacga taaaatccgg cgcgatacat cacttgccat
    12841 aagtcattga tgcgctatgg atcctgacca atcaaatagt cacccagctc gtgaactgcc
    12901 gcttccaccg tgcgggcgcg gccttcaatc actggctcgc cccagccgac gacgccttca
    12961 tcggtttcaa ttttcaggaa catccagcgg ggaggtaaac gatacgtggt aattttggtg
    13021 attttcattg cactgcctct cgatacgcct taacaaatgc tgctgcctgc tgcgcggtgc
    13081 gttctacgga ctgcccggcg cgatagagat cgctgcctaa gcccgcccca gcacaacctg
    13141 cgtctatcca ctgcgccagg ttttctggcg tcacgccgcc aacggcaaag actgcgatgt
    13201 ccgatggcaa taccgctttt aacgctttga tgtattgcgg accaaaagcc gatgacggaa
    13261 atattttcag cgcctgcgcg cccgcttcga gcgcggtaaa ggcttcggtc gccgtcgcgc
    13321 agccggggca gacggtcatg ccgtagccca ccgcacggcg gatcacttca ctatggatat
    13381 tgggcgtaac gatgagctga cagcccatcc tggcgagcgc atcgacctgt tcaggtttca
    13441 gtaccgtacc tgcgccaatc aacgccttgt cgccatacgc atcaacgatg gcgggaatgc
    13501 tttgctccca ttgtggggaa ttcagcggga tttcaaccgc gtcgaacccg gcgtcaatca
    13561 ccgcgccaac atgcgccagc gcctcgtcgg gcttaatgcc gcgcaaaatg gcgatcagcg
    13621 gaagtttagt ttgccactgc atgagcgatg ctccttatac cagcctgaaa tgccgtgtcg
    13681 cccgccaccg ccgtcacgtc gcaacccatc gcctgaaagg cttgctggta gcgcgcggtc
    13741 aacgatgttc cggcgacaag ggtgatggcg tgttgatggg ccacatagtc gcgcatactg
    13801 gcgacctctg cgccaatcaa caaaccagag agaaattcac tgacctgttc acggggaagt
    13861 gttcccagca catgcgaggc acgaacttca aaaagctgcg gcaatatggc gggcgtatta
    13921 agaccacgct caaggccagc cgtgaaggca tcggcagagt tttcctgcgg cggcaaacct
    13981 gcaccaatca atgagtgatt taacagtaaa tgatgtaatt caccggtcat cacggtgcga
    14041 aaatcgttga tttgctggct atcggcctgc acccatttgc aatgcgtacc gggcatgaca
    14101 taaagagagg aaggagccag agctcgcgcg ccgatcaatt gagtttcttc gccgcgcatc
    14161 acattgtggt tatcatcatg agagacgcat aatccgggaa taatccagat attgtcgcca
    14221 acagacgtta attgttcgcc aatagacgaa aaatgggcag gaacagataa atacggagca
    14281 actttccagc cgacattgct gccgaccatt cctgccatta ccactggcgt attctcttca
    14341 cgccagtcgg tcgtgacttc tgctaacacc gcagccggag attttccgtt caggcgcgtg
    14401 acgcctgctt ctgattgcct gctctccagg cagtggtcgc cctgataaag ccaggcgcgc
    14461 agattggtcg atccccagtc aattgcgatg tagcgagctg tcatgtgatt tcctttaacc
    14521 ttcgtgtcga gctggcgatc atggtaagcg ccgcctgctc tgccgcatcg ccgtcctgat
    14581 gccgtatcgc atcgaacagc gccttatgtt cctggagcgt ttgcggcatg ttggcctcat
    14641 cgcccatcca ggttcgttca aaaaccgccc gctgcagcga actgatcgca atgctaagtt
    14701 gctgtaacac cgggttatgc accgactgca gcaccgcctc gtggtagcga atatccgctt
    14761 cgttaaacgc ttcgcggtcc tgattgttgg caatcatctc gttcagcgcc gattcaatct
    14821 gcgccagatc gctggaggtc gcgcgctctg ctgcccaacg ggcaatcgcc ggttccacca
    14881 gatttcgcac ttcgctcatg gcactgataa gccgtgggtc gtagtcgttt tccagcaccc
    14941 attgcagtac gtcagtgtcg aggtaattcc actggttacg cggtgccaca aacgcgccgc
    15001 gataacgttt catttcaatc agccgtttcg ccatcaacga acggaacacc tcacggatga
    15061 tgttgcgcga ggttgcaaac tcctcacaga gttccgcctc agccggaagc ggcgagcctg
    15121 gtacgtattt gccgtgaacg atctgtttac ccagcgtaat gacgatgcga tcggttttat
    15181 tgagagtcat ggagagtcct tgtgctctgt atgttcttct ctactttacc ccgatcgatg
    15241 cataatcagg aaagtatcaa tcgcagtcag acaccatcac ggggctatcg caggcaaagg
    15301 atagtaatgg aacaaaaggc tatgtttttg taggggaatc gctggattac cttatcacag
    15361 atggtgccga tgacatcgtt aagatgctca aggatccagc tcttaaccgg cacaatattc
    15421 aggttgctga tgacgcaaga tttgttttaa atgcggggaa aaagaaattt accggcacaa
    15481 tatcactcta ttacaactgg agcacagaag aagaaaaagc acgagtgaca caatatcgtt
    15541 ttgcttgtgg tgttcagcac tgtacccgga cagtagaaaa cctggaaggt actatccatg
    15601 agaaaaataa aaacatggat tactcaaacg taatggcttt ctaccatcca tttaaagtgc
    15661 gattttatga ataccattca cccagaggca ttccggatgg tgtttccgca gcattactgc
    15721 cagtgactgt tacgctggac atcattactg caccgctgca atttctggtt gtatatgcag
    15781 taaaccaata atcagtaagc gggcaaacgc gtttatgctg tttgcccgcc cacagattaa
    15841 ttcagcacat acttctcaat agcaaacgcc acgccatctt caaggttaga tttggtgaca
    15901 aagttcgcca cttctttcac cgaaggaata gcgttatcca tcgccacacc gacgcctgca
    15961 tattcaatca ttgcgatgtc gttttcctga tcgccaatcg ccatgatttc ttccggttta
    16021 atacctaaca cgtcggccag tgatttcaca cccgtacctt tgttaacgcg tttatcgagg
    16081 atttcgagga agtacggcgc acttttcagc acggtatatt tctctttcac ttcctgcgga
    16141 atacgcgcga tagcctggtc gaggatggcg ggttcatcaa tcatcatcac tttcaggaac
    16201 tgggtattgg ggtccatttt ctccgcttcg cagaacacca gcggaatagt ggcaacgaaa
    16261 gattcatgca ctgtgtagta gctgatatcg cggttggcgg tgtacagcgt ggtgcggtcc
    16321 agggcgtgga aatgagaacc gacttcgcga gagagttttt ccaggaaacg atagtcgtca
    16381 tagctgagag cagtttgcgc cacggtgcta ccatcagctg ccttctgtac tagcgcgccg
    16441 ttataagtaa tgcagtagtc gcccggctgt tccatatgca gctctttcag gtagttgtgc
    16501 acacctgcat acgggcgacc cgtcgttagc acgacattca cgccacgggc gcgagctgcg
    16561 gcaatcgcat ttttaacggc gggtgaaatg gtgtgatcgg gcagcagaag ggtgccatcc
    16621 atatcgatag caatgagttt gatagccatg agttccccag gtagattggt tcctgaccca
    16681 tgctaacgcg attccgctca aaaatcagta caacacccga gggaaaaggg ggatgcaacg
    16741 cgggttgcat gctccccatt tgcttagcgg aagagtttcc ctttcagcag ttccatacct
    16801 gcggaaagca gatcgttatt ggcttgtggt gacacttcac cttgcggtga gagcgcatca
    16861 ataatcttcg gcaattgctc tgccagtaaa ctggaagctg tactggtatc cacgccaagt
    16921 ttttgcccga gatcggacac cgcatttgtg ccgagcgccg attccagttg ctcgccacta
    16981 accgattgat tgccctgttg attactcagc caggttgaga gaatggcccc taagccgcca
    17041 ctttgcagtt tttccagcag cacctgaatg ccgccctgct cctcaaccca acttaaaata
    17101 gcctgatatt tccccgcatc gcctttcaga aaggcaccga caacttcatc aaaaagcccc
    17161 atgataatca cctgtaaagc gttacgtgtt gacccaaaaa gtatagattt gcgggatgat
    17221 aattgcggat tgcagaaata aaaagggcgg agatgatctc cgcccttttc ttatagcttc
    17281 ttgccggatg cggcgtgaac gccttatccg gcctacaaaa tcatgaaaat tcaatacatt
    17341 gcaagatttt cgtaggcctg ataagcgtag cgcatcaggc acgctcgcat ggttagcgcc
    17401 attaaatatc gatattcgcc gctttcaggg cgttctcttc aataaacgca cggcgcggtt
    17461 caacggcgtc gcccatcagc gtggtgaaca actggtcggc agcaatcgca tctttaacgg
    17521 taacgcgcag catacgacgg ctttccgggt ccatggtggt ttcccacagc tgttccgggt
    17581 tcatctcgcc cagaccttta taacgctgga tggagaggcc gcgacgggac tctttcacca
    17641 gccagtccag cgcctgctcg aagctggcta ccggctgacg acgctcgcca cgttcgataa
    17701 acgcatcttc ttccagcaag ccacgcagtt tctcacccag cgtgcagata cgacgatatt
    17761 cgccaccggt gataaactcg tgatccagcg gatagtcagt atccacaccg tgggtacgca
    17821 cgcgaacaat cggttcaaac aggttttgtt cggcattggt gtggacatca aacttccact
    17881 ggctgccgtg ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc cagcgggtaa
    17941 cggtctgctc atcagagagg tcggcttccg tcagcgtcgg ctgatagata agctctttca
    18001 gcattgcttt cggataacga cgctccatgc ggttgatcat tttctgcgtc gcgttgtact
    18061 cagacaccaa tttctctaac gcttcgccag ccaatgccgg tgcgctggcg ttggtgtgca
    18121 gcgttgcgcc atccagcgcg atagagatct ggtactgatc catcgcttcg tcgtctttaa
    18181 tgtactgttc ttgcttgcct ttcttcactt tgtacagcgg cggctgagcg atgtagacgt
    18241 gaccgcgttc aacgatttcc ggcatctgac gatagaaaaa ggtcaacagc agcgtacgaa
    18301 tgtgcgagcc gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa cgcagtttgt
    18361 ccgggttgta ctcgtcacga ccgataccac agccaagcgc ggtgataagc gtcgccactt
    18421 cctgagaaga gagcatctta tcgaagcgcg ctttctcaac gttgaggatt ttacccttca
    18481 gcggcagaat cgcctggttc ttgcggttac gcccctgctt cgcagagccg cccgcggagt
    18541 ccccttccac caggtacagt tcggaaagcg ccggatcgcg ttcctggcag tctgccagtt
    18601 tgcccggcag gcccgctaag tcgagcgcac ctttacggcg ggtcatttca cgcgcgcgac
    18661 gcgccgcttc acgggcacgg gcagcatcga taattttgcc aaccacgatt ttcgcgtcgg
    18721 ttgggttttc cagcaggtat tctgccagca gttcgttcat ctgctgttca accgctgatt
    18781 tcacctcaga agaaaccagt ttgtctttgg tctgggagga gaatttcggg tccggcactt
    18841 tcacggaaac gaccgcaatc aggccttcac gcgcatcgtc accggtggcg ctaactttgg
    18901 cttttttgct gtagccttct ttgtccatgt aggcgttcag ggtacgggtc atcgccgcac
    18961 ggaagcctgc caggtgagta ccgccgtcac gctgcggaat gttgttggta aagcagtaga
    19021 tgttttcctg gaagccatcg ttccactgca acgccacttc gacgccaata ccgtcttttt
    19081 cagtggagaa gtagaagata ttcgggtgga tcggcgtttt gttcttgttc agatattcaa
    19141 cgaacgcctt gatgccgcct tcatagtgga agtggtcttc tttgccgtcg cgcttgtcgc
    19201 gcagacgaat ggaaacgccg gagttgagga acgacaactc acgcagacgt ttcgccagaa
    19261 tttcatattc gaactcggtc acattggtga aggtttcgag gctgggccag aaacgcacca
    19321 tggtgccggt tttttcagtc tcgccggtaa ccgccaacgg tgcctgcggt acaccgtgtt
    19381 cgtagatctg acggtgaatt ttaccctcgc gctggataac cagctccagt ttttgcgaca
    19441 gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc ggacacttta taggagttat
    19501 cgtcaaattt accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc gatacgccct
    19561 cttccgggtg aataccggtc ggaatgccgc gcccgtcatc ctgtacagag acagagttat
    19621 cggcgtgaat ggtgacgata atttctttac agtgacccgc gagcgcttcg tcgatagcgt
    19681 tatctaccac ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg tcgccgatat
    19741 acatacccgg gcgcttacgc accgcatcca gccctttcag gactttgata ctggaggagt
    19801 cataagaatt cgacatcaac gtttctcgct catttatact tgggttaatc cgttatttta
    19861 cccttttcca cggtaaacat cttcgaattt tcgtccgaca tgtctataac gtgttcagca
    19921 ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta agcggctggc aagcagcccg
    19981 cgacgctcat catcaagctc agaggcaaaa tcatctatca ggtagagaca ccgccgcccg
    20041 ctttcacggg tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa cagcttaagc
    20101 tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg taagtccgct
    20161 ttatgcgggc catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt acgttccagc
    20221 acctcagcat attctgtctc tttctcccag ccgcgctgga aagagaaagt cagagaaaac
    20281 tcagggagaa attgcttaca agtatcggcc atatcagccg cgataccggc gctatactcc
    20341 gcgcgccagg tgctgatttg ctccgccagc gggatcagct ctttatccca cgggcgtagc
    20401 tgttcgtaac gtgtcacctg gcgcagcgcc gcattgcgct gcttgagcaa tcgcttgaga
    20461 ttgctccagg cggtgaaaaa tccgggttcg ttgtgaaagc atccccagtc gaggaatgct
    20521 cttctgtatt tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat caactgcatt
    20581 ggcatcaggt gcgccagttc cgcgacctta tgcccgtctg taccgtcgat gcggactttg
    20641 ctgtcgccct gtttgtcttt ggttaagcca atcgctgtct cgcgctcttc gccctgtaat
    20701 cgcccgtgga gaacaaacgc ctcctgctca tggcgaatga cgcgaccaat ctgcaaactg
    20761 cgaaacgccc gaccatggcc gagcgtatag atggcttcca gcacgctggt tttgccactg
    20821 ccgttggcac ctaccagaaa gttaaagccg ggagataaag cgagatccgc ggtttcaatg
    20881 ttgcggaaat cgcggatcaa caagcgggtg agggacatta cagtctcatt ggcatgacaa
    20941 cataggcagc cgactgtgat gcggcatctt caatctgcac gctggaaacc gaatcggtca
    21001 gcatcatgcg gacgttttca catttcagcg cgttcagaac atccagcacg tagctgacgt
    21061 tgaagccgat ttccatctcc gcaccgctat aggtaacgtc gaggatctct tccgcttctt
    21121 cctgttccgg gttgttggcg gtgattttca gctggttttc gctgacataa agacgtacgc
    21181 cgcggaattt ctcgttagag agaatcgccg cgcgagcaaa cgcctgcttg agcagatcgc
    21241 agccagcttc cagatgtttg tccgggttct tcggcagaac gcggcgataa tccgggaagc
    21301 gaccatccac cagtttggag gtgaagataa agtcgccaac gtgggcgcga atgttgttgc
    21361 tgccaatctg tacgcgcagc ggattgtcgc cgccgtcgag catacgcatc agttcaatca
    21421 cgcctttacg cggcacgatc accgaatggc ttggcaaaga ttgaccaatt ggcattgaac
    21481 agactgccag acggtggccg tcggtcgcca cggtgcgcag ttcttcgcct tcggtttcaa
    21541 acagcatacc gtttaagtaa tagcgaacgt cctgatgcgc catagaaaac tgggtcgctt
    21601 caatcagacg cttcatcgtc gcctgcggca gggtaaattc gacttcgctc tgccagtcat
    21661 cgaggttcgg gaaatccgcc gctggcaggg tagacagcga aaaacggcta cgcccggagc
    21721 gcaccagcat ccgttcacct tccagctgta cggcaatttc cgcgccttca ggcagaccac
    21781 ggcagatatc aaagaatttg cgcgccggaa cggtcgtcgc tcctggctcg tgtggctgaa
    21841 ccagcgcaac gcgtgccacc atttccatct cgagatcagt accggtcagc gacaacgtac
    21901 cgtcagcaac ctgtaacagc agattaccga gaatcggtag cgtaggacga ccacctaacg
    21961 gcccgctcac ctgttgtagc ggttttaata aatgctcacg ttctacggta aatttcatag
    22021 gtttacgatg acaatgttct gattaaattt gaaaaatctt ctttgatatc gtggctctct
    22081 tcacgcaact gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc acgaccacca
    22141 aacgcatcgc caatctccgg cagactgtgg ttagtcagct ctttcgccag cgccatcgcc
    22201 atctggcgcg gacgcgccac cgagcgggat cgacgcttgg aaaggagatc cgcgactttg
    22261 atcttgtagt actccgccac cgtcttctga atattgtcga tggtgaccag tttttcctgc
    22321 aatgccagca agtcgcgcag tgcctcacgc acgaagtcga tggtgatcgc ccgtccggta
    22381 aagttggcgt tagcaataac gcggttcagc gccccttcca gctcacgtac gttagatcgt
    22441 agacgcttgg cgataaagaa cgccacttcg cctggcaaac gaatgtcatt ttcgtcggcc
    22501 tttttcatca ggatcgccac gcgggtttcc agctctggcg gttcgatcgc cacagtcagt
    22561 ccccaaccga agcgggattt caaacgatcc tcaacgccgt tgatctcttt cggatagcga
    22621 tccgaggtga gaatgatctg ttgattacct tccagcaggg cgttgaaggt gtggaaaaac
    22681 tcttcctgag atcgttcttt attagcaaaa aactgaatat cgtcgatcag cagtgcatct
    22741 acggaacggt agtagcgttt aaactcttcg atcgcgttgt tttgcagggc tttaaccatg
    22801 tcctgaacaa agcgctcgga gtgcatataa accactttgg cattcggctt gcgcgccata
    22861 atgccgttac ccaccgcatg cagcaggtga gttttaccca gacccgtgcc gccataaagg
    22921 aacaatgggt tataggcacc gccagggtta tccgccacct ggcgagccgc cgcgcgcgcc
    22981 agttggttag atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt tacgttagaa
    23041 cgataggtcg gttctgccgg ggccgggacg ttatcccaac ctgagcgcgt agaaggcgca
    23101 gcacgttgcg gctgcgtttg cgccacctgt gcaggggccg cgacgttgct cgtcactgcc
    23161 gcttgtggcg tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg tggggcatcc
    23221 gctccgcaga aactggttag cagtccattg atattattaa ggtacttgtc ccgtacccaa
    23281 tcgaggacaa aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag ttccgcctgc
    23341 aatgggcgta tccacatact gaattctgtg gctggtaact catcctgcaa tcgggcaaga
    23401 cactgctgcc aaagcgaaag tgacacggcg gactccactc gaacaaaagt cgataatgac
    23461 taaggctgaa acattcatga ttgttgacgt acgtcgaaaa gaccctgctt gagggtgacg
    23521 cacgaaccgc tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga tcgggaccgc
    23581 ggatcatagc ctaaactgcg caagagatct tctgtttctc acagattttc ccgatttatc
    23641 cacaggactt tccagacctc gctaagtgta aacgatcctg ccgcgaggcg ggcacgattt
    23701 acgccgcata ttggaaaatt taatgaccat agacaaaaat tggcttaatc gatctaataa
    23761 agatctagga cgatccttgc gctttaccca tcagcccgta taatcctcca cccggcgcgc
    23821 catgctggtt ttcactggtg tgaggtcgta cattttccct gcgaaaaggt gcggaaaagc
    23881 gcggtaaata aggaaagaga attgactccg gagtgtacaa ttattacaat ccggcctctt
    23941 taatcaccca tggcttcggt gtccatcgtt tcatttttcg gcggatatcc aataaagcca
    24001 ttgaatttat tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa ccgtctgtac
    24061 tgaagcgcaa ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat ggtcgtcagg
    24121 ttctggcacg tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag taataaagct
    24181 aacccctgag tggttaagct cgcatttccc agggagttac gcttgttaac tcccagtcaa
    24241 ttcacattcg tcttccagca gccacaacgg gctggcacgc cgcaaattac cattctcggc
    24301 cgcctgaatt cgctggggca tccccgtatc ggtcttacag tcgccaagaa aaacgttcga
    24361 cgcgcccatg aacgcaatcg gattaaacgt ctgacgcgtg aaagcttccg tctgcgccaa
    24421 catgaactcc cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc cgacctcgat
    24481 aaccgtgctc tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg cctggctcgc
    24541 gggtcctgat agccctcatt cgggtctatc aacgcctgat tagtccgcta ctcgggccgc
    24601 attgtcgttt cactccaacc tgttcaagct acggaattga ggcattgcgc aggtttggag
    24661 tgataaaagg cagttggttg acggtgaaac gcgtattaaa atgccaccct ttacaccctg
    24721 gtggtgacga tcccgtcccg cccggaccat ttgataccag agaacactaa cgatggattc
    24781 gcaacgcaat cttttagtca tcgctttgct gttcgtgtct ttcatgatct ggcaagcctg
    24841 ggagcaggat aaaaacccgc aacctcaggc ccaacagacc acgcagacaa cgaccaccgc
    24901 agcgggtagc gccgccgacc agggcgtacc ggccagtggc caggggaaac tgatctcggt
    24961 taagaccgac gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg agcaagctct
    25021 gctgcctgct tacccgaaag agctgaactc tacccagccg ttccagctgc tggaaacttc
    25081 accgcagttt atttatcagg cacagagcgg tctgaccggt cgtgatggcc cggataaccc
    25141 ggctaacggc ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc tggctgaagg
    25201 tcaaaacgaa ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca cgtttaccaa
    25261 aacgtttgtc ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg tgcagaacgc
    25321 tggcgagaaa ccgctggaaa tctccacctt tggtcagttg aagcaatcca tcactctgcc
    25381 accgcatctc gataccggaa gcagcaactt cgcactgcac accttccgcg gcgcggcgta
    25441 ctccacgcct gacgagaagt acgagaaata caagttcgat accattgccg ataacgaaaa
    25501 cctgaacatc tcttcgaaag gtggttgggt ggcaatgctg caacagtatt tcgcgacggc
    25561 gtggatcccg cataacgacg gtaccaacaa cttctatacc gctaatctgg gtaacggcat
    25621 cgccgctatc ggctataaat ctcagccggt actggttcag cctggtcaga ctggcgcgat
    25681 gaacagcacc ctgtgggttg gcccggaaat ccaggacaaa atggcagctg ttgctccgca
    25741 cctggatctg accgttgatt acggttggtt gtggttcatc tctcagccgc tgttcaaact
    25801 gctgaaatgg atccatagct ttgtgggtaa ctggggcttc tccattatca tcatcacctt
    25861 tatcgttcgt ggcatcatgt acccgctgac caaagcgcag tacacctcca tggcgaagat
    25921 gcgtatgctg cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg acaaacagcg
    25981 tatcagccag gaaatgatgg cgctgtacaa agctgagaag gttaacccgc tgggcggctg
    26041 cttcccgctg ctgatccaga tgccaatctt cctggcgttg tactacatgc tgatgggttc
    26101 cgttgaactg cgtcaggcac cgtttgcact gtggatccac gacctgtcgg cacaggaccc
    26161 gtactacatc ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga agatgtcgcc
    26221 gaccacagtg accgacccga tgcagcagaa gatcatgacc tttatgccgg tcatcttcac
    26281 cgtgttcttc ctgtggttcc cgtcaggtct ggtgctgtac tatatcgtca gcaacctggt
    26341 aaccattatt cagcagcagc tgatttaccg tggtctggaa aaacgtggcc tgcatagccg
    26401 cgagaagaaa aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt cagttgaccg
    26461 ccttttttct tttcgtaggg tggataagca ccgcgcatcc gccacacaaa gcaacaggaa
    26521 catcatgagc gataatgaca ctatcgtagc ccaggccacg cctccgggac gtggcggcgt
    26581 tggcatcctg cgcatctccg gcctaaaagc ccgtgaagtt gccgaaaccg tgctgggtaa
    26641 actgcctaag ccgcgctacg ccgattatct tccgtttaaa gacgccgacg gcagcgtgct
    26701 cgatcagggg attgcgctat ggttccctgg cccgaactcg ttcaccggcg aagatgtgct
    26761 ggaactgcag ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac gcattctgac
    26821 cattcccggc ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt ttcttaacga
    26881 taaactcgac ttagcccagg ccgaggcgat tgccgatctt atcgacgcca gttcggaaca
    26941 ggcggcccgt tcggcactta actcgctgca aggcgcattc tccgcacggg tcaaccatct
    27001 ggtggaagcc ctcacccact tgcgcattta cgtcgaagcg gcaattgatt tccctgatga
    27061 agagatcgat ttcctctccg acggcaaaat tgaagcccag cttaatgacg tgattgccga
    27121 tctcgatgca gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag ggatgaaggt
    27181 ggtgattgcc ggacgtccta acgccggtaa atcgagcctg ttaaacgcgc tggcgggccg
    27241 tgaagcagca atcgtaactg atatcgccgg aaccacacgt gacgtgctgc gtgagcatat
    27301 ccacattgac ggaatgccgc tgcatatcat cgataccgct gggctacgtg aagccagtga
    27361 cgaagtggaa cgtattggta tcgagcgcgc gtggcaggaa attgaacagg ccgaccgcgt
    27421 gctgtttatg gtcgatggca ccacaacaga cgccgtggat ccggcagaga tctggccgga
    27481 atttatcgcc cgtctgccag cgaaactacc gatcaccgtg gtgcgcaata aagccgatat
    27541 caccggcgaa acgctgggaa tgagtgaagt gaacggtcac gcgttaattc gtctctcggc
    27601 aaggacaggt gaaggcgtgg aggtgctgcg taaccatctc aaacagagca tgggctttga
    27661 caccaacatg gaaggcggct tcctggcgcg tcgtcgccac ctacaggcgc tggaacaggc
    27721 agcggagcat ttgcagcaag gtaaagcgca actgttaggt gcctgggcgg gtgaactgct
    27781 ggcggaagag ttgcgcctgg cacagcagaa cttaagcgaa atcaccgggg aatttacctc
    27841 tgatgatttg ctggggcgga ttttctccag cttctgtatt ggtaagtaac cgcgcttacg
    27901 aagccgtatt ctgactgtca gatgcggctt cgcttcattg ttagcactcc tgttattcct
    27961 caaccctttt tttaaacatt aaaattctta cgtaatttat aatctttaaa aaaagcattt
    28021 aatattgctc cccg
//
LOCUS       DAJTVJ010000049        27436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000049 DAJTVJ010000000
VERSION     DAJTVJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.049"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(171..1610)
                     /gene="rsmF"
                     /locus_tag="OO448_002019"
     CDS             complement(171..1610)
                     /gene="rsmF"
                     /locus_tag="OO448_002019"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6337227.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1691..4324)
                     /gene="yebT"
                     /locus_tag="OO448_002020"
     CDS             complement(1691..4324)
                     /gene="yebT"
                     /locus_tag="OO448_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6337228.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4293..5576)
                     /gene="yebS"
                     /locus_tag="OO448_002021"
     CDS             complement(4293..5576)
                     /gene="yebS"
                     /locus_tag="OO448_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6337229.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5706..6203
                     /gene="msrC"
                     /locus_tag="OO448_002022"
     CDS             5706..6203
                     /gene="msrC"
                     /locus_tag="OO448_002022"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6337230.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6300..6998
                     /gene="proQ"
                     /locus_tag="OO448_002023"
     CDS             6300..6998
                     /gene="proQ"
                     /locus_tag="OO448_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6337231.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7018..9066
                     /gene="prc"
                     /locus_tag="OO448_002024"
     CDS             7018..9066
                     /gene="prc"
                     /locus_tag="OO448_002024"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6337232.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9258..10139
                     /gene="htpX"
                     /locus_tag="OO448_002025"
     CDS             9258..10139
                     /gene="htpX"
                     /locus_tag="OO448_002025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6337233.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10185..11248)
                     /locus_tag="OO448_002026"
                     /pseudo
     CDS             complement(10185..11248)
                     /locus_tag="OO448_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11425..12216
                     /gene="kdgR"
                     /locus_tag="OO448_002027"
     CDS             11425..12216
                     /gene="kdgR"
                     /locus_tag="OO448_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6337234.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12359..12598)
                     /locus_tag="OO448_002028"
     CDS             complement(12359..12598)
                     /locus_tag="OO448_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337235.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12757..12900
                     /gene="mgrB"
                     /locus_tag="OO448_002029"
     CDS             12757..12900
                     /gene="mgrB"
                     /locus_tag="OO448_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6337236.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            12975..13262
                     /locus_tag="OO448_002030"
     CDS             12975..13262
                     /locus_tag="OO448_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6337237.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13932..14075
                     /locus_tag="OO448_002031"
     CDS             13932..14075
                     /locus_tag="OO448_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6337238.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14088..14297
                     /gene="cspE"
                     /locus_tag="OO448_002032"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14088..14297
                     /gene="cspE"
                     /locus_tag="OO448_002032"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6337239.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14463..15272
                     /gene="rlmA"
                     /locus_tag="OO448_002033"
     CDS             14463..15272
                     /gene="rlmA"
                     /locus_tag="OO448_002033"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6337240.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15269..15835)
                     /gene="mntP"
                     /locus_tag="OO448_002034"
     CDS             complement(15269..15835)
                     /gene="mntP"
                     /locus_tag="OO448_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6337241.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16264..16722)
                     /locus_tag="OO448_002035"
     CDS             complement(16264..16722)
                     /locus_tag="OO448_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6337242.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16777..17628)
                     /gene="manZ"
                     /locus_tag="OO448_002036"
     CDS             complement(16777..17628)
                     /gene="manZ"
                     /locus_tag="OO448_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6337243.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17641..18441)
                     /gene="manY"
                     /locus_tag="OO448_002037"
     CDS             complement(17641..18441)
                     /gene="manY"
                     /locus_tag="OO448_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6337244.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18504..19475)
                     /gene="manX"
                     /locus_tag="OO448_002038"
     CDS             complement(18504..19475)
                     /gene="manX"
                     /locus_tag="OO448_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6337245.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19938..21488
                     /gene="yoaE"
                     /locus_tag="OO448_002039"
     CDS             19938..21488
                     /gene="yoaE"
                     /locus_tag="OO448_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6337246.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21492..23090)
                     /locus_tag="OO448_002040"
     CDS             complement(21492..23090)
                     /locus_tag="OO448_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6337247.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23221..24585)
                     /gene="sdaA"
                     /locus_tag="OO448_002041"
     CDS             complement(23221..24585)
                     /gene="sdaA"
                     /locus_tag="OO448_002041"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6337248.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24769..25347)
                     /locus_tag="OO448_002042"
     CDS             complement(24769..25347)
                     /locus_tag="OO448_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6337249.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25351..26712)
                     /gene="pabB"
                     /locus_tag="OO448_002043"
     CDS             complement(25351..26712)
                     /gene="pabB"
                     /locus_tag="OO448_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6337250.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26786..26965
                     /locus_tag="OO448_002044"
     CDS             26786..26965
                     /locus_tag="OO448_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6337251.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27085..27408)
                     /locus_tag="OO448_002045"
     CDS             complement(27085..27408)
                     /locus_tag="OO448_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6337252.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7060 a         6758 c         6931 g         6687 t
ORIGIN      
        1 tgtaattcgc cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg
       61 ctctccattt agctgccggt tgtggtacga ccagtccgat cgcaccattt ttcagcctag
      121 ttgaggcagc cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcaggcgtta
      181 ccggtaaaaa gtttcccatc gcgcaccagt tcacgcggat agctgttttt caatcgcgaa
      241 ccaatccgtt tggctaaacc aatcggttga tgctggaaag taaccaacac gtcatccgcc
      301 actggcgcgg cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcctgcggt
      361 gtcagttcaa aagcgttcac attgtcgggg gtggcaaggg caataactgc ttcatgctgc
      421 cagcgataac ctttgttgtg cgtttcggca agtttaatcc ccaagcgaga aaatcggact
      481 ttaccgatca gggcttcaat gcccaccggg aacaaccaca gttctttgtc acgctgccag
      541 aggcgcaggt tttcatccca gtttaagcca acacctgcag ccgcctgacg aatttgtcca
      601 gcttctcgat ctttcactgg gctgaacgga aaattaccga ctttgtattt gggggcgggt
      661 aaggcgggaa tcgcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa
      721 atttgtggga aaacatgcaa aaagccttcc tcggtcagcg ccttgtttgc accagggaag
      781 agatcaccaa gcggtaaaaa ctctactgcg tcggggtaag tctctttcag ccacaggcaa
      841 acggcttcgt tttcttcctg gtttaaggta caggtcgagt aaaccagcgt accgccaggg
      901 cgtaatgcat gaaaggcgct gtcgatcagt tcccgttggg tagctgcgat ttcctgattg
      961 ctttctggtg accagttttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag
     1021 cagggagcgt ccagcaaaat ggcatcaaac atttctggca ctgccgcacc aaacacgcgg
     1081 ccatcaaaat gtgtgagcgc aacattactg ataccacagc ggctgatatt ggcatgtaac
     1141 acttttaccc gactggcgga aaactcattg gcaagaattg ccccttcgtt attcatccgc
     1201 gcggaaattt gcgtcgtttt ggagccaggc gcggcagcga catccatcac ccgctgtggt
     1261 gcattaccgt cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa
     1321 aacaggccac ttaaatgctc ggcggtacta cccaatggca atgcatcttc attgtcgcgt
     1381 tcaatccaga aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg
     1441 gttaattgca ggaaatcagc aacggagatt ttcagcgtat taacgcgaat gctgcggcgc
     1501 aacgggcgct gacaggcggc aagaaaatca tcaaaagaga gcgtcgaagg catcgcttcg
     1561 cgcatttgcg tcagaaaggc gtccgggaaa taaacggtgt gttgggccac gagcatatac
     1621 cactggcaaa aaaaacaggc gcgcagttta gcataaacgc tccggcgcag gcacgccgga
     1681 gcagtgggca ttatttggga agcgcagtac cccattcacg ccactctttc ggttcacttt
     1741 cctgcaacag gaagtgcttg ccttcctggg ctttcggtgc cagtggcgta cccggaggcg
     1801 tggcgaaggc gatgccgcca cggataaact ggttaaaggt gccggttttc actacgccgc
     1861 ccgtcagacc aaagtccaga ctgtaacccg atgccaacca gaagacggaa ttgttacgca
     1921 ccaggtgttg atagcgttta ctgatgcgca tcgcaatcat cacgcgatct gacaatgtcc
     1981 ccagcgtcat tccggtaacc gtacctactt ccagaccacg gaacagcaca ggcgtaccaa
     2041 tacctaacga accggcttcc ggtgcttcga caataatgct taagccatcc aggtaacgcg
     2101 aatcagtaat agtagcttct tgcaactcaa agtcacggcg aggattaccc cgacctggtt
     2161 cgacattgat atacggctgg aggatagtat ctagatgctc aacgcccgcg gctgaaattt
     2221 gcggtgtgac caccgagaag cgcgtaccgc cgcgggcgaa ggtctggaca tactccggat
     2281 agagcaccgc ctttgcctgt acttcattgc gcgcggtaat cagatccagc gtctggattt
     2341 gcccgatatc aataccgaga tagcgaattg gcatcccgac cgccagtttt ccggcatcga
     2401 aagcgtgaag cgtaatctgc ccaccaaccg cacgggccgc tgtttcggaa gcatacagaa
     2461 ttcgtttgtc gcctttacgc tgactggcgc tggcaccgct gaggttatcg aagctaatgg
     2521 ctccctttaa tgctctggag agcggggatg cctgtacggt caggccacta ccattcagct
     2581 gaactttcgc cccgccttct gcccagaaca cgctattgct ggtcagaagg ttgcgatact
     2641 ccggcttaat atgcagatcg atatcaaacg cgttagctcg cggacgcacg gtaatcactt
     2701 caccaacttc aaatttacgg tacagcacta ccgatcctgc ctgcacatcc ggcagcgtct
     2761 ctgcactcaa actcacggtt gtggtgggta aatcgctaag gctgttctcc agcgcttttt
     2821 ccagattggc atacagtgga tagctggctt tcatctcacc tttatcgcct ggcagaatac
     2881 gtatcccgcc gttaatccat tctgaggcgc tggcaccaag aaactcaacg ccatccagcc
     2941 ccaccttcac gtcgacacgg ctgttgacga caaatttgct atcgcctttt accagttcgc
     3001 gatgctgagg ctcgatggcg acggtaaagg taacgccttt gctggtgagt ttacgatcaa
     3061 taacctggcc tacctgcacg ccgtgaagaa tgagcggctg accagcatca ataccgtaac
     3121 tttccggcgc ggtcagggtc agcgtcagaa catcaggttc atgcagcagt gctttttcgc
     3181 ctggcacaac aacgaactct ttgcgtggct cgccatcgcc gggtaccaac tcgaaggttt
     3241 tgccagtcag cagggcgctg agattggcat cgctaaggga taatttcgga ttaagtaatt
     3301 cgatgcgggt attttcacga agcagggtaa cgacgctggg attaactgtc atttccccag
     3361 tgactttacc accaggattt aaatccagtt tagttagctg tccgacttcc agtccctgat
     3421 acattaatgg cgtcgagtcg gcggttaatc cggccccact cggcagttcc agtttgatta
     3481 ttacgccacg ctggctatgg gcgaggtctt catacagacc aaaggtatct tccgcctcgg
     3541 caggtttcga ctcttcaggt gaatcgaagg caatcgcacc gttaaccagt gccgccagac
     3601 tttccagttt caccttcgca ccactgatac tgacgttggc atcaacgccg gaaacgttcc
     3661 agaaacggct accttttttc actaggtcgg taaaacgccg ctcgatcagg acatcaatca
     3721 ccacgccttg cttgttggga ttgatggcat agtcgtagac ttttcccaca gggatcttgc
     3781 ggaaatagac caatgaaccg ctgttcagcg aaccgagatc gggggcttgc aggtggatca
     3841 tcagatcgcc attgtccagt cgatatttcg gttgggtatc gagtgcgaca aagtgatcct
     3901 gctctttacc tttacctggc atcatgccga tatagttacc accgacgagg gcgtccagcc
     3961 cggagacacc tgccaacgat gcttttggcg tcaccagcca gaactgagtc tcttcgcgca
     4021 gcgcatcttt catatcggac ttgatgctga ccttgacttc aatcttacga agatcgtcgc
     4081 tgaggctgat atcctgcact gttccgactt caacgccctg ataacgaaca ggcgtacggc
     4141 ccgggacaat accatccgcc gacataaagt cgatggtgac ggtattaccc cggtcctgat
     4201 aactgtccca aatcagccaa ccggcaatca ttagcgcgat gaaaggcagc agccagaaag
     4261 gtgagatacg gcgtttattt ttaatctgcg cttcagtcgt cgaagcgggc gtttcctgac
     4321 tcatgtgcat cccaaagtaa gcggctgtcc agccattcca cagcaagaat agtcaaaatt
     4381 acggctgcgc caaaataaaa cgcagccggt cccatagtaa aagcgaggat ctgatcgcga
     4441 ttaatcagcg acatggttaa agatatgaca aacaggtcga gcatcgacca gcgtccaatc
     4501 caggtcacca tccgcagtaa cagaatgcgt gtgcgtaaac cttgctggca tttaaaatga
     4561 atgctcaaca gtaaagtgaa catgacgatc actttagtaa acggtaccag aatactggcg
     4621 ataaacacga ttccggcgac cgcaatgttg ctacttgcca gcgacataat cccggaaaga
     4681 atcgtatctt cctgccggcc accattcaga taaatgatag aaataggcaa taggttggct
     4741 ggtaacaaca aaacgattga cgctaacagc gccgcccagc atttttgcaa actatgacga
     4801 cgacgcaggc gtagcgggat atggcaacgc gggcagcgac cacgctgatc aggatagccg
     4861 gtaaaatggc acccaagaca gacacgaagt ttctcgtccc tacgcgtagc ggggcgctgc
     4921 ggataatatc gctcccacag ctcctcaaca ttaagatgtg acaacgtcac cgtcgttaaa
     4981 atcaccaacg ccacaaaaga gaacaagccg acacccgcct ggatatgggc ataatcctgt
     5041 acctttatag aagcaacgcc aatgccgacc aggtagatat ccagcattac ccactcttta
     5101 agacgctcaa gcatcagcag taccggacgt aaattcattc ccagtcggtt accaaaccat
     5161 aaatatgcta tggaggacac cagaatgagg ggggcaccaa taacgcagaa aaagaccatc
     5221 gcccccgtta tcgtatcgcc ctgtttggtc atttgccaga tgccttgcat gacgttggcg
     5281 tcgatacgaa tacctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcatc
     5341 agcaacagca tggtgaaagc cattgccgcc aggcgcgtta gcgaccagtc gcgcccgtcg
     5401 cgaatttttg cctgacagcg cggacaatag gcactctgat gagaatttat ctcgggcagg
     5461 ctaaacagca tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg
     5521 attgccctca ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg
     5581 tgccaaatgt aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat
     5641 taacgcattt aatgcttatt ttaataggct ggcagttcca ggtaagacaa acaagtgatt
     5701 atataatgaa caaaacagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg
     5761 cgggagaaac cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tacgagcgtc
     5821 tcactgacgt aaactgggca ggtttttatt tgcttgagga cgatacactg gtactcggac
     5881 catttcaggg caaaattgcc tgtgtccgga tacccgtcgg gcgcggggtg tgcggcactg
     5941 cggttgcccg caatcaagtg cagcgtatcg aggatgttca cgcgtttgac gggcatattg
     6001 cctgtgatgc ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta
     6061 ttggtgttct tgacatcgat agtaccgtct tcggtcgctt tacagacgag gacgagcaag
     6121 gcttacgtca gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat
     6181 tctttgcgag cgtcgcagga taatcaacgg ataacgtagc atttactggt agcgtcatta
     6241 taatgacgcc tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca
     6301 tggaaaatca acctaagttg aatagcagta aagaagtaat cgcgtttctg gccgaacgtt
     6361 ttccccactg tttcagtgcg gaaggtgaag cgcgtccgct gaaaatcggt atttttcagg
     6421 atttggtcga tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt
     6481 tacgtctcta cacttcgagc tggcgttatc tttacggtgt taaacccggc gcaacgcgtg
     6541 tcgatcttga cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca
     6601 agcagcttga agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa
     6661 aacgcgaagc tgccgcagct gctggtgaga aagaagacgc actgcgccgc gaacgcaagc
     6721 cacgtccgac cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc
     6781 cggtagagaa agcgccaaaa acagtaaaag cacctcgcga agaacagcac accccggttt
     6841 ctgacatttc agctctgact gtcggacaag ccctgaaggt gaaagcgggt caaaacgcga
     6901 tggatgccac cgtattagaa atcaccaaag atggcgtccg cgtccagctg aattcgggta
     6961 tgtctttgat tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg
     7021 aacatgtttt ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc
     7081 gctgtagaag atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat
     7141 gcgacggtaa gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac
     7201 ctcgatcagg cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc
     7261 cacaacgtgc tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc
     7321 gatgaactgc gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag
     7381 cgccgttttg agcgttacca gtacgctttg tcggtactgg aaaagccgat ggatttcacc
     7441 ggcaacgaca cttataacct tgaccgcagc aaagcgccct ggccgaaaaa cgaggctgag
     7501 ttgaacgcgc tgtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctggcagga
     7561 aaaacggata aagaaattcg tgaaaccctg acacgccgct acaaatttgc cattcgtcgt
     7621 ctggcgcaaa ccaacagcga agatgttttc tcgctggcaa tgacggcgtt tgcgcgtgaa
     7681 atcgacccgc ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg
     7741 agtttgtcgc tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc
     7801 aattcgatgg tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa
     7861 attgtcggtg ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat
     7921 gatgtggttg ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct
     7981 gctggtaaag ggaccaagac ccgcactgta acattgaccc gtgaacgtat tcgtctcgaa
     8041 gaccgcgcgg ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat
     8101 attccgggct tctatgtggg tttgacagac gatgtcaaag ttcaactgca gaaactggaa
     8161 aaacagaatg tcagcagtgt gatcatcgac ctgcgtagca atggcggtgg ggcgttgacc
     8221 gaagcggtat cgctctcagg tctgtttatt cctgcgggtc ccattgttca ggtccgcgat
     8281 aacaacggca aggttcgtga agacagcgat accgacgggc aggtgttcta taaaggcccg
     8341 ctggtggtac tggttgaccg tttcagtgct tcggcttcag aaatctttgc cgcggcaatg
     8401 caggattacg gtcgtgcgct ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag
     8461 caataccgtt cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg
     8521 ggttctgtgc agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt
     8581 aaaggcgtaa cgccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag
     8641 aaattcgaag ataacgcgct gccgtgggat agcattgatg ccgcgactta tgtgaaatca
     8701 ggcgatttaa cggcctttgg gccggagctg ctgaaggaac ataatgcgcg tatcgcgaaa
     8761 gatcctgagt tccagaacat catgaaggat atcgcgcgct tcaacgctat gagggacaag
     8821 cgcaatatcg tttctctgaa ctacgctgtg cgtgagaaag agaataatga agatgatgcg
     8881 acgcgtctgg cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaag
     8941 ctggatgatc taccgaaaga ttaccaggag ccggatcctt atctggatga gacggtgaat
     9001 atcgcactcg atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgctcccgtc
     9061 aagtaatatc aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg
     9121 taaatcagat gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga
     9181 cttgaaaata gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact
     9241 gaggtaaaaa gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa
     9301 tggtcgtttt cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc
     9361 tgatgatcat ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca
     9421 aatggatggc attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg
     9481 aacgttggct ggtcaatact gtagcaaccc aggctcgtca ggcagggatc gctatgccgc
     9541 aagtggctat ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg
     9601 cctctctggt tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg
     9661 cggtaattgc tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga
     9721 ttcagggcgt ggtgaacacc ttcgttatct ttatttcccg tattctggcg cagcttgccg
     9781 cgggttttat gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct
     9841 actttgcggt tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca
     9901 tgtggttctc gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc
     9961 gcgagaaaat gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa
    10021 ccagcatgat ggctttctgc attaacggta agtcgaaatc gctcagtgag ttgttcatga
    10081 ctcacccgcc gctggataaa cgaattgaag ctctgcgtac gggtgaatac ctgaagtaat
    10141 atgaagtaaa tactaaaaag cgcgtcctga tcgacgcgct ttttttatgc cttggatcgt
    10201 ggctgagtga tacgtaaacc actgacacag gcagcaatca ctgccagaat agccgcagcc
    10261 atcagcgaaa cgtgcgtacc attatcaccg aactgattta gcatcagcgc caccagtgcc
    10321 gcgccgctac tctgacccag tagacgagcc gttcctaaca tgccactggc tccaccgcta
    10381 cgttcgcgag gagcggaggt aataatggtg tggttattgg gtgactggaa caagccaaat
    10441 ccagcgccac ataagatcat cggccagata atattgatat ccgcaggtga cgcgggcagc
    10501 agaaccaggg gaaaaagccc cgcagccatg atgaacaacc ctagagcacc cagcaatcct
    10561 gcatgtacgc gttcaatcaa atagcctgcc aacggagcca tcaccatcgt tgctaacggc
    10621 cacggtgtca gaagtaaacc tgtttcgact tcactacgcc cgagcacggt ttgcaggtaa
    10681 aagggcaggg aaaccattgc cagcatttgt gcgcagaaag agcaaacaga tgtgcaaata
    10741 gaaagtgaaa acagcgggat acgcagtaaa tccaccggta gcagcggtac gggaagagaa
    10801 agctggcggc gaataaagaa aataccaaca acaaccatta ccaccagttc cgcagcaatt
    10861 aacgtcagcg attgcccctg agcgaaacca ctcaacgcag tgataagcag gccgaaggtt
    10921 aacgcgttca tcacggcgct gggcaggtcg aaacggggtt tactggcgcg agaaccattg
    10981 ggtggcagaa aacgcatcgc cagaagcagg gcgataatac ctaacggtac gttgattaaa
    11041 aataaccatt tccaggatgc gatggagagg attgctgcag caattgtcgg cccggcagca
    11101 gaagagacgg caacaatatg gcgattgttg gcagggcgac gttggcgatt gcgccgtcaa
    11161 ggacagccat cgaaatgcca atcacaatgg ttaatatcgc accgtatcgc tggggcaatg
    11221 gcaggccgtc ggcctgaact tttggcatag gaattttata tctttggtga ataatcaaca
    11281 ggggtatgat tctagcactg atattcacgg tggatgtcgc agaattgtaa ctaaccggca
    11341 aaagatggat tgcgaccacc ctggtgcgaa attataataa aaaacagttc tgatttttat
    11401 aaaacactcg caatgaggtg ataaatggct aacgcagatc tggataaaca gcctgattct
    11461 gtatcttccg tgctaaaagt ttttggcatt ttgcaggcgc tgggtgaaga gcgcgaaata
    11521 gggataaccg agctgtcgca gcgcgtcatg atgtcaaaaa gcaccgttta tcgcttttta
    11581 cagaccatga aaaccttagg ttatgtggcg caggaagggg agtcggagaa atattccctg
    11641 accctgaaat tgtttgaact gggcgctcgc gcgttacaaa acgtcgattt aattcgtagc
    11701 gcagatatcc agatgcgtga gatctcccgc ctgaccaaag aaactatcca cctcggcgca
    11761 ctggacgaag acagtattgt ttacattcac aaaattgact ctatgtacaa tttgcgcatg
    11821 tattcacgga ttgggcgtcg taatccgctg tacagcaccg cgattggtaa ggtactgctg
    11881 gcatggcgcg atctcgatga agtgaagcaa attcttgagg gcgtggagta taaacgcagt
    11941 accgagcgga ccatcaccag tacagaagcg ttattacccg ttctggacca ggtgcgcgag
    12001 caggggtatg gcgaagataa cgaagagcag gaagaagggc tgcgatgcat tgcggtaccg
    12061 gtatttgatc gctttggcgt ggtcattgcc ggtttgagca tctccttccc gacgttgcgt
    12121 ttctctgaag agcgtttaca ggaatatgtc gcaatgttgc ataccgcagc gcgcaaaatt
    12181 tctgcccaaa tgggttatca cgactatccg ttctgatgag agtaagaacc tgtcggaata
    12241 tcaaacagac aggttcttta tttagcatga gaaaaataaa gttgaaggtg cgttatatta
    12301 aacgcgcttg ctataagaat attttactca tgagtgagaa tctggttgtt tattaccctt
    12361 aaccattatc gaccacgata ttgcttttac gtaacagtgg gcaatctgtt atccccaaga
    12421 aaccactttt agtgtgcaag tattgtaccg tgctggtgcc tctggcagtc agataggtac
    12481 attgcaaacc taatcctgcg gcattctctt tgcttccaat caaaacgcca tatccgctga
    12541 gtaataatcc tatccatacc agtgctatca gcataactgt gcgaatgatg aatcgcatta
    12601 caacctcttc tctttttatg ttcgcttaat cgtagcggca atatgcgctg aagcaagcta
    12661 ctcattccga aaaagcacga atatcgacat agttaggcgc tgtttaacta acgcatgcta
    12721 gtttaatgac ataaggtagg tgaagcggag attggagtga aaaagtttcg atgggtcgtt
    12781 ctggttgtcg tggtgttggc ttgcttgctg ctttgggcgc aggtattcaa catgatgtgc
    12841 gatcaggatg tacaattttt cagcggaatt tgtgccatta accagtttat cccgtggtga
    12901 tatcattttt tcagcactag attttcttcc tctccagcag tggtagaata gcgcctctac
    12961 tcagaggtgg tgaaatgaac gaagttgtaa attcaggcgt gatgaacatt gcgtctttgg
    13021 ttgtatcggt ggtggttctt cttatcgggc tcatcttgtg gttttttatc aatcgtgcca
    13081 gttcacggac taacgaacag attgaactgc ttgaggcgtt gctggatcag caaaaacgtc
    13141 aaaatgcact gttacgtcgt ttgtgcgaag caaacgaacc agagaaagca gataaaaaga
    13201 ccattgagag tcaaaaatcg gttgatgacg aagatattat tcgcctggtc gccgaacgat
    13261 aatccttcct caatcttgag taaccccgtc gttgaagggt tactcaattc atcctgatta
    13321 tacgcactgt catggtgcaa ctgcttcaaa ttaaaactct ggtctgactt caaataatta
    13381 tcatattatt tatttgcgta acgggatatc agaaaaagat attttcctca ttaatttatt
    13441 aggatgttta catcggattt gtgattaagc gtggtattat ttattagcga aacgtttctc
    13501 tcttgatttt tttgctcatt catcaatttt tcttatttta aattttcaat ccctttgggg
    13561 attgacttct ctttagggta attgatagcc gttaaccgac tgttttatga gaaaaagtga
    13621 tataactttt tattcattgc atagcaaaaa atgtgatatt gcacgcacta tgtaataact
    13681 tctcccactg gcctggaaca actgaactta ttgaactatg ttagaaaata cgccagttta
    13741 agtatctgcc tgaactggca aggttaagca caatgatata tcggcgcgta ttccgttgca
    13801 taagtgtgca aaaaaagtgg aagacgtatc gagatttgtg cgtctgatcg agacatgttt
    13861 aaaaatggct tgccataatt aacgttgtat gtgataacag atttcgggtt aaacgaggta
    13921 cagttctgtt tatgtgtggc attttcagta aagaagtcct gagtaaacac gttgacgttg
    13981 aataccgctt ctctgccgag ccttatattg gtgcctcatg cagtaatgtg tcagttttat
    14041 ctatgttatg cctgcgggcg aagaaaacaa tctaaggaat ttttcaaatg gcaaagatta
    14101 aaggtcaggt taagtggttc aacgagtcta aaggttttgg cttcattact ccggctgatg
    14161 gcagcaaaga cgtgttcgta cacttctccg ctatccaggg taatggcttc aaaactctgg
    14221 ctgaaggtca gaacgttgag ttcgaaattc aggacggcca gaaaggtccg gcagctgtta
    14281 acgtaacagc tatctgatcg aatccactga tctgaagtgt gaatacgctt caatctcgct
    14341 ataaagcctc gtcgaatgcg aggcttttta ctatgcttta tcttcgctcc tggcgttcgg
    14401 atatttgccc gccgcgtgat tcgcgttaca cttgcggcct ttagtatcct gccggagttg
    14461 tcatgtcttt ttcctgtcca ctttgccatc agcctctttc gcgtgaaaaa aacagctata
    14521 tctgtcccca gcgacatcag tttgatatgg cgaaagaagg gtatgtcaat ctgctgtccg
    14581 ttcagcataa acggtctcgt gatccgggcg acagcgcgga aatgatgcaa gcacgccgcg
    14641 cattcttaga tgccggacat tatcagccgc tgcgtgatgc aattgtcgcc caactgaggg
    14701 aacggcttga tgataaggcc acggcggtgc tggatattgg ctgtggtgaa gggtattaca
    14761 cacacgcatt tgccgatgcg ttgcccgaaa tcaccacgtt tggtctggat gtttcgaagg
    14821 tagcgataaa agcggcggcg aaacgctatc cgcaggtcac tttttgtgtc gcttccagcc
    14881 accgtttgcc gttttccgat accagtatgg acgccataat acgtatttac gcgccgtgta
    14941 aagcagaaga attagcacga gtagtgaagc ccggcggctg ggtcattact gccacgccgg
    15001 gaccgcgaca tttgatggag ctgaaggggc tgatttacaa tgaagtacat cttcatgcac
    15061 ctcatgcaga acaactggaa ggttttacat tacagcagag tgcggagttg tgttatccga
    15121 tgcgtcttcg cggtgatgaa gccgtcgcat tattgcagat gacgccgttt acctggcgtg
    15181 cgaagccaga agtctggcaa acactggcag caaaagaagt gttcgactgc cagacggact
    15241 ttaatattca cctctggcag cgttcttatt aaccgtggaa gtgcgtccag aggatctgga
    15301 cgccgatgcc gatcagtacc agcccgccga gaatttccgc ttttttccca ataattgagc
    15361 cgataaagcg accaaccatc atccctaatg ttgacataat caaggttgca caaccaatgg
    15421 ccaatgcggt cgcgataatg ttgacctgca ggaaagcaag accaacaccc acagccatgg
    15481 catccaggct ggtggcaatc gcggtggtta ccagtagcca gaaaccgtgt cgacggcgcg
    15541 gctcttcatc ttcatcatct gcgccacgaa aaccctcaat aatcattcgc ccgccgagga
    15601 atatcagcag cacaaacgca atccagtggt tccattcaag gacaaaccgg ctggctaaca
    15661 tgcccattcc ccagccgatc agcggcgtca gggtttcgac ggcaccaaaa ataaggccgg
    15721 ttcgcaatgc ttcagaaaat ttcggtttat ggagggtggc acctttaccg attgatgcag
    15781 caaatgcatc catcgacata ccaaacgcaa gaagaacagt agcagtgata ttcatgacaa
    15841 tgtcctgacc ggggtatcca taaaacacat catcgccccc agcaaacatg catacatgaa
    15901 caacaagccg tgatggtttt cgcgttcatg caggcatagc gattgatgtg catatggtct
    15961 cgcctgactg aaagtattca gtccgtacgt gccacgtttt gcaacgagta tgttgacacg
    16021 tacatttccg gaatctggaa atcggctact ccccaaatga gggcgcaacc ttaacatatt
    16081 ttgagaatat aaaacaactg ttggaatata ttatttcatt gctttattga taacgatttt
    16141 catttggatt tatatatcaa tgaaacgtta aaataaataa caaataaggg aagaggtaat
    16201 atagcctaag ctatgtctga ttattaaaaa tacactaaac atagctttgg ctaaattcat
    16261 aacttattga gttgaaacga gaagcttata aattttttcc agatcgtcga tatttcgaac
    16321 gcggattaac agccgacgct gttctaattg catcaccaac acgccatctt ccgacaagtt
    16381 catcgctttg attcggctat attcaatcca gacattggcg aagaaaaaac ctttttgttt
    16441 aaagatgatc ttcggaacgc ggatccagaa tatataaaaa cccatcagag ccagtgcgct
    16501 taataaccat gtcgttatta acgcaccatg attcgtgacg ttgttataga taagaatgac
    16561 aatcaatccg acgaagataa cgctatcgat gcgaccacgc cggagcaaag gaattgccag
    16621 cagggtgggg ccgttacggc ggggcatgat gaactgatcg tagatcgcga aggccaggag
    16681 tgcggcgatg aaaagaatca gtaccaggtc cgtgattgtc attaatcctc cagataaaaa
    16741 aacggggcca aaaggccccg gtagtgtaca acagtcttac agtcccagca ggccgcaagc
    16801 gtaaccagcg ataccgatga cgaagaagcc aacgatgatc cacagcgggt taactttttt
    16861 gcgcagtagc cacatacaag caaaggtcag cagcagtggt accaggcctg gcattaactg
    16921 gtccagaata gtctggacag tagtaacgtg ttctttgccc gtctggtcag taatgcgaga
    16981 gacaaccagc gggatgttga catgtgtcca cttgttaacc aatgccccca tgacaaacag
    17041 gccgaggata gacgcccctt ccgtcagttt ttgcaggaag ccaccaccca tatctttaac
    17101 gatatcgata cctttggagt aaccatacgc tacgccgtag taacgggttg ccagacgcac
    17161 caggttaaac aggatgaaga acagcagcgg acctaacagg ctgccgctca tcgcgatacc
    17221 ggcacccagt gctgcaaata ccggacgtac ggttccccag aagatcgggt cgcctacacc
    17281 agccagcggc cccatcaaac cgactttgat accgttgata gcaccgtcgt cgatctctgc
    17341 gccattagca cgctgttctt ccagcgccag ggttacgccg agaatcggcg cagccacgaa
    17401 cggctgggtg ttaaagaact ccaggtgacg gcgaatagct tgtttacgag cttcgttgtt
    17461 ctcagggtag aggcgacgaa ttgccggtac catagagaag cagaaaccca gtgcctgcat
    17521 acgttcgaag ttccatgaac cctggaagag gttagaacgc aggaagacgc cacgaatatc
    17581 actttgagtg agttttttct cggtggtagt ttgagttgta tcaaccattt cgctcacctg
    17641 ttagtccagt tcgttatcga gatcgttgtt accagctgcc tgagcaggcg caccggctac
    17701 gcggttgtat ttcgggctaa gttggatgta gagcactgcc ataacagtac caatcacacc
    17761 cagagcaacc aggttaaagt tggtgaatgc tgcggttacg aagccgaggt agaagaacgg
    17821 catcaggtag ccagcacgca tcatgttgat aaccatcgca taaccaacca ccacgatcat
    17881 gccaccggcg atattcagac cattggtcac cacttccgga atcgcattca gcatgttctg
    17941 tacttcgctg gtaccaacag acagcgcaac gatgacggcc ggaatagcca cacgcattgc
    18001 ttgcaggaac agagaagaaa cgtggatcca ggaaatcgct gtcaggttgc cgttatcagc
    18061 agccttatcc gcagcgtgct ggaaagcaac ggtaatagta cgaacgatga tggtcagtac
    18121 ctggcccgca gcggccagag ggattgccag tgcgatacct gcaccaatgc tctgatgacc
    18181 tgcgataacc agaatggtag aaatgataga agccagagcg gcgtcaggcg caactgcagc
    18241 accgatgttc atccagccca gcgcgatcat ttccagcgta ccaccgataa taataccggt
    18301 tttcatatcc ccaagaacga tacccaccag ggtacacgcg attagcggac ggtgaaactg
    18361 aaattcatcg aggattgatc ccatacctgc gatacaggct acgataaata ccagcacaat
    18421 ttgaagagtg gtaatctcca ttgtacttct cctgtttacg taagacttaa gtgtgaaaac
    18481 tgagtgataa tcaacacaat acgttactta tcgattttgc tgatcagatc catcattttc
    18541 agtttcggat cggtggaaac cttacggact tccagctcaa taccgcgcgc attcagtttc
    18601 ttgaacgcct cgatatcttt ttcatcaacc gaaaccgcgt tattcacctg ggttttaccc
    18661 tgacggaatg ccataccacc gacgttaaca gaggtgattt tcacgccgcc ttcaacgaga
    18721 cgctctacat ctgttgggtt ggtaaataac agcattacgc gttcgccagc atatttcggg
    18781 ttgttgtaga cgcgaatcat tttggcaaca tcaactacgt gtgctgttac gcccggaggt
    18841 gcaacctggg tgagcagtgt cttacgaacg gtatccgcag ccacttcatc actaacaaca
    18901 ataatacggg agacattggt ttctttggtc cagcgggtgg cgacctgacc gtgaatcaga
    18961 cggtcgtcga tacgcgcaag gccaataacc atgtagtcgt ttggccccat tggttttgcc
    19021 ggagttggag ccgcttttgg tgctgcggca gcgggtgctg gcgcggcttt ttcaaccggt
    19081 ttggctttca gtgctttcac gccttcacgg cctgtttcta ctgccagcgc aaccagttca
    19141 tcaaagcttg ggtcatcatc acgagccatt aacgtttcca cgagcattgg aatgttaacg
    19201 cctgcaatga cttcataatg ctctttgtcg acgacaatgc ggctggcagc attgaacggg
    19261 ctgcctcccc atgtatcaac gagaaacagc acgcctttag tggtgtcgag ttttgccaac
    19321 tgagcgttgt acttttcaat cagcgtttcg gcattttcac ctggaacgaa atcgatccag
    19381 ccgacgtttt cctgctcgcc taacagcatt tctgccgttt taagcaactg ctctgcagcc
    19441 caaccatgtg tgcctataac aatagcaatg gtcacttgct acctccttta ttatcgttaa
    19501 cacctcaacg tgccagatgt atttttgaat cgtcgtccac aatcgaatcg attcagataa
    19561 gggctacaac caaaaacccc tgcgtttcgc gatttatttt agatatcgaa aaaattattt
    19621 tatgtgatga agatccgtaa tttaactttc gattagcaga aatttcgaaa ggtaaaatat
    19681 ccttgtcaca ttcgtttgca aaggaaggta aatctttgcc aaatcagagg cgtctctgat
    19741 atgtttaact cccgtttaac aaccatggag tatagggcag tagcccgcag tatggatcgt
    19801 caccgacgtc atttcagcat caggcctttt aacgcctgcc tttctggcac tctatgccgc
    19861 acctttcgtt tgcattttgt cgttacgcct gcattatttc tggcgtcgaa tagctattcc
    19921 ttaagcagga gcttgtcatg gaattcttaa tggacccctc aatttgggcg gggctactca
    19981 cgcttgttgt tctcgaaatt gtgctgggta tcgataacct ggtcttcatc gccattcttg
    20041 ctgacaaact gccgccaaaa caacgcgata aagcgcgttt gctggggtta tcactggcgc
    20101 tgattatgcg tctggggctg ctgtcgctga tttcatggat ggtcacgctg accaaaccgc
    20161 tatttaccgt catggatttc tccttctccg gacgcgacct gattatgttg ttcgggggga
    20221 tattcttgct gttcaaagca acaaccgaac tgcatgaacg gctggaaaac cgcgatcatg
    20281 attccggcca cggtaaaggc tacgccagtt tctgggtggt cgtcacacag attgtcatcc
    20341 ttgacgccgt cttctcgttg gatgcggtaa ttactgcagt agggatggtt aaccatctgc
    20401 cggtgatgat ggcggcggta gtgattgcga tggcggttat gttgctggca tccaaaccgc
    20461 tgacgcgatt cgttaaccag caccccacgg tggtggtgct ctgtctgagc ttcctgttaa
    20521 tgattggtct gagtctggtg gcagaaggtt tcggtttcca cattccgaaa ggttacctgt
    20581 atgccgcgat tggcttctcg atcatcatcg aagtgtttaa ccagattgcg cgtcgcaact
    20641 ttattcgcca ccagtcgact ttgccgctgc gagcgcgtac tgccgatgcc atcctgcgtt
    20701 tgatgggcgg gaaacgtcag gccaatgttc agcacgatgc cgataacccg atgccgatcc
    20761 cggaaggtgc atttgccgaa gaagaacgtt acatgattaa cggcgtactg acgcttgcgt
    20821 cgcgttctct gcgcgggatc atgacgccgc gcggtgaaat aagctgggtt gacgctaatc
    20881 tcggggtcga tgaaatccgc gagcaactgc tctcttcacc gcacagtctg ttcccggtat
    20941 gtcgcggtga actggatgaa atcatcggta ttgtacgtgc taaagaactg ctggtggcgc
    21001 tggaagaggg cgttgatgtg gcggcgattg cttcggcgtc tccggcgatt atcgtcccgg
    21061 aaaccctcga tccgatcaac ctgttgggcg tgctgcgtcg tgctcgcggg agctttgtta
    21121 tcgtgaccaa cgagtttggt gtggtacaag gtctggtcac gccgctggat gtgctggaag
    21181 ccattgcggg tgaattcccg gacgctgacg aaacgccgga aatcattact gatggtgacg
    21241 gctggctggt aaaaggcggt acagatttgc atgccttgca gcaggcgctt gatgttgagc
    21301 accttgccga tgacgatgat atcgcgacgg tcgcgggcct cgtgatctcg gcaaatggtc
    21361 acattccccg tgtgggcgat gtgattgatg tagggccact gcatatcacc atcattgaag
    21421 ccaatgatta tcgtgttgat ctggttcgca ttgttaaaga gcaaccggcg cacgatgaag
    21481 atgagtaagc attaacgtaa cggcataatg ggcgtgatat gtccattatg ccgggcgggc
    21541 ggcggttggc tgcccgccag ccatttggga aaatcacgta gcggcatcgg ctgtgcgtac
    21601 aaataccctt gcaaaatatg cacgccatga cggcgcaaat acttcgcctg ttcttgcgtt
    21661 tccacgccct ccgccaccag ttcaatattc agtctttgcc ccagcgcgat gatgatatcg
    21721 gtcaccgtcg agttaaccgc gtcagaacct atagctgcgg taaatgactt atcaattttc
    21781 agcatgtcag gacgtaatgt ttcaagccag gaaaatgaac tgttgccggt gccgaagtca
    21841 tcaatcgcca gtttgacgtt tttacgatgt agctcgcggg caatccgata atcaacatcc
    21901 agtaaggcat cgcgttcggt gatttcgagg atcagttgct gaatcgggtg agcgctaaac
    21961 cagtactgat tgagatcttt tatcagcaca ccacggcgaa aatggctggg tgcgacgtta
    22021 atgccaacat gaaactgact actcatcggg aaaacatggc gctgacgaat ggtttctgcc
    22081 atcacatagc gggtcagtgg cacaattaaa tgatgttctt ccgcgatagg aataaacaca
    22141 tccggtgaaa tccagccctg acgcggattg ttccagcgca gcaaaatctc tacaccaata
    22201 cattgctggc tgcgcgcatt aagcaaaggc tgacagaaca attcgaactc atgttgcgcc
    22261 aggcccagat tgatttcgcg ggaaaagctc atccggtaag cggtcgccag ccaggcgatg
    22321 tagcccacca gtaaacttag cagcaccgcc agcggtaatt gtgttggaag atagtgccat
    22381 gccagcgccg tagcccccgg accattaacg ttaatggtaa aaggaaagcg ttgcgaagaa
    22441 acctgatagt tttcattgtc ttcaggttgc ggaagggaat ctaccagccc attaccatag
    22501 agcaaatgcc gtttgtccac cgtcaggctg gcgctactga tttgcggcag ttgtggctca
    22561 aggagcattg ccgtcagtaa gtcgatgtta atcatctcca tgaccccagc attgctactg
    22621 cccgctgcag gcgtccattg aatcaaaacc ggactgcctt taatcagggc acggtcgatc
    22681 gtcaggcgta aaagtggttg cggtgcagga agttcagcca gaatgtccac gacgggcaca
    22741 ttgcgataac caaaaatgct ggagcaatag agtgttccgt cttgcaccag gccaatggat
    22801 cgcacagttt ggagttttgc cgcctgttta cgtaatggca aatgggcgac agagcagggc
    22861 agaccaatca gcggaagcaa gacttcgcta cctgcctgta ggggaagcag tactttatct
    22921 aattcctcta cagcgtgatt ggcgaattgt actacccgtt gttgatttaa gttacgctgt
    22981 gaaataaatc gcacgctcag ggtcgaagcg agcgtaacga gtgcgcaaat cgtacaaaca
    23041 atcattcgat tacggcgata ggttttaatg atccgttgtg ctttttgcat gcgggccacc
    23101 ctgtaagcgt atggcgacaa acgcctgacg caacagtgga agtgtagtgg ggaatattac
    23161 agcagacgag aaagcgggta taaattcgcc catccgttgc agatgggcga gtaagaagta
    23221 ttagtcacac tggactttga ttgccagacc accgcgtgag gtttcgcggt atttggcgtt
    23281 catgtcctta ccagtttcgt acatcgtttc gatgacctta tccagcgaga cgcgcggtgc
    23341 actggtgcgg cgcagagcca tccgtgcggc gttaatcgcc ttcacagagg caatggcatt
    23401 acgctcaatg cacggcacct gaacctgccc tgcaaccggg tcgcaggtta aaccaaggtt
    23461 gtgttccatg ccaatttccg ccgccacgca aacctgttcc gggctaccgc ccagcagttc
    23521 tgcaagaccc gcagcagcca ttgaacaggc aacacccact tcgccctggc aaccaacttc
    23581 cgcaccggaa atagaggcgt tcattttata caatgcacca atcgcgcccg ctgccataaa
    23641 gtaacgggta tagatgtccg ggctgaccga ttcaataaag tggtcatagt aagccagcac
    23701 tgccggaacg ataccgcagg caccgttggt tggcgcagtt accacacgac caccggcggc
    23761 gttttcttcg ttaactgcca gcgcaaacat gtttacccag tcaatgacat tcatcggatc
    23821 gttagacagt ttatcgctgg aaactaacat ccggcgcagg gcagacgcac gacgtggcac
    23881 gcgcagcggg cctggcagta caccttcggt gttcatcccg cgatcgatac atgcctgcat
    23941 ggtttgccag acatgcgcga aatactcgtc gatctctttc ttgctgtgca gcgccagttc
    24001 gttctgcata gcgagaccag acagcgaata gccggtttca ttacagtacg cgagcagttc
    24061 ggtggcagat ttgaacggat acggcacgct cacttcgttg gcagcatcct gaccaaagtg
    24121 ttcttcatcg acgataaaac cgccgccgat ggaataataa gttttgctgt agacgacttc
    24181 atcgccgtta taggcgtgga tttgcatacc gttttcatgc agcggcaggt tgccgttatg
    24241 aaaacgcatc ccgttgtcgc gcgggaaatc cacttcatgc cgtccctgtg ccagcagcag
    24301 acgttcgcgc tcttctacgt cgcgaataaa accgggaata ctgtcgatat ccacggtggc
    24361 aggttcgtta cctgcaagac ccataataat ggcgatatcg gtgtggtggc ctttacccgt
    24421 cagcgacagt gaaccataaa cgtccacggc aacgcgagta acgctatcca gtaagccttt
    24481 ttcgaccaga tcatcgacga actgtttacc tgccttcata ggccctacgg tatgggaaga
    24541 tgagggacca atccccacct taaacatgtc gaatagacta atcacgataa tactcctgac
    24601 aaggtgactg gacttccagt aacgaactaa taacagcgca tagtgtaaga gggaacgcgg
    24661 cgactggctt aactattcac atgaattaaa ctaatcaaaa accgcgattt aactaatatt
    24721 gcgatgattg tctcaagtag tgttttgtag atgtaaagat aagtatagtc agggtttcac
    24781 accaatttgc agcgccagct cacgaattat gcctgcggtc attccccata caaaatactg
    24841 ttcgtaccag gacagccata cccgatgtga atcgccacgg cggtagatat ctaaagggtg
    24901 ataacgaccc agatgtaatg cctgggcgag cggcatttca aacaccgccg agacttcatc
    24961 ttcactggcg cgatacggca gatcgggcgg gataatgccg accactgggg ttacctgata
    25021 gccagttacg ctatcgacgg gcggcagcac gccgataact tcaacggcgg aaggcggtat
    25081 agcgacctct tcttcagctt cgcgcagcgc ggcggcgata actgatgcgt ccgtgtcatc
    25141 gactgcacct ccagggaatg ccacttgtcc agcgtgttta cgcagatgaa ttgaacgctg
    25201 agtcagcaac aaccccggtt gcggtcgacg gacgatgggg attaacacgg cagcctgacg
    25261 atgatttagg gtttcccggt taatttgcgg gcgcaaaagt tgaaagcgcg ataaaaaatc
    25321 atcaagcatc aggctacggt attccacgtc ttacttctcc agttggcgta atatcttatt
    25381 aactttatca aaagtttcct gatattccgc ttcttcctgg ctatcggcga caattccacc
    25441 gcccgcagag cagtatattt gtccgttaat ggcagtcaga gtgcggatag taatgctggt
    25501 atccatgttg ccgcaaaagc tcaaatagcc aatgctgccg caccaggcat tacgtcgctg
    25561 cggttccagt tcgtcgataa tttccatagc ccgtactttc ggagccccgg ttattgagcc
    25621 accaggaaaa gctgcgcgca gcagatcgct ggcgtgtaac tgttctggta gttgtgccgt
    25681 tatggtgctg accagatgat gcacggcagg gaagggttcc accacgaaca gctctggtac
    25741 ttttaccgaa cctgctacgg caacacgacc gatatcatta cgcattaaat cgacaatcat
    25801 cagattttcg gcacgatctt tcgctgagtt cgccagtttt tctgcttgtt tgctatcttc
    25861 ctgaggatcg ggcaggcgtg gtagcgtgcc tttaataggg cgggtctgga tttcactatt
    25921 atcacaaaga ataaaccgct ctggcgaaag gcttaaaatt gcaccctgtt caagacgtaa
    25981 aaaagcgcta aatggcgcgc ggttggcctg attaagctga aggaatgcct gccattcatc
    26041 gccagaatag gtcgcatgaa aacgctgggc gagatttacc tgatagcaat caccgctgtg
    26101 cagatattcc tgtacctggc gaaatttttc gccgtactgc tcgcgggtca tattggattg
    26161 ccagtcggaa gtaagcgtga aatcttcctg cggcgagaat tgctggcttt ccagccaggc
    26221 ccgacgggca ttgacatcat tatgactcag caaagaaact gtatgacgct ggtggtcgac
    26281 aatgagcgcc caatcgtaga tacccactgc catatccggc agaacgatat cttgttccgc
    26341 aatttctggc agtgactcaa aacggcggcc cagatcgtag ccaaacaacc ccagtgcgcc
    26401 gccctgaaat ggcaaatctt cgttatgcgt tgggcgaatg tctgcgcgat ccagcacctg
    26461 ctggagcacc tgtagcgggt catcagtggt cgttgtgcgt ttttcgcttt cactaacaac
    26521 ggtttcttta cctaaagtgg ttaaagtgca aatcggatcg gcgaccacaa tatcaaagcg
    26581 gctatacgga tgatcggcat agccggagtg taaaagcatc gcccacggca ggtggcttaa
    26641 gcgggagaaa taaaattcag cggcgtcctg acgccagggt aaagtaatca cagcgggaga
    26701 taacgtcttc atcagtcctg actctactgg ctatgtgccg ggtagcatga aataattagc
    26761 gccaacaatt tagcaggagt taacaatgtt tgcaggttta ccttcactca cccatgaaca
    26821 gcagcaaaaa gctgtcgagc ggatccagga actgatggca caagggatga gcagcggcca
    26881 ggcaatcgcg ttggtggcag aagagctgcg tgccaaccat tccggcgagc gaatcgtggc
    26941 gcgttttgag gatgaagacg agtaacacac taatccgtat tgatttcgtt atacaagccg
    27001 atgattattt gattgtgaga aaaaggacgc tttttatatg tgattaaaag cgtcctgatc
    27061 catttatgct gatgtaaatt ttactcagac cagtgctttt agttcttcct gcatatatgt
    27121 tgaaaagtat tcaggatttt taatcacaga gcgttcacct gactccattt tttttgccca
    27181 tccaaccatt ttttccgtaa acccagggtt ccagccttcc tggtcagctc gcgccgtaat
    27241 atcagcggca ctcgcaggta ctcccagttc ttttaaatat ttaaagattc ccttcgccgt
    27301 gctttcattc atcgaacttg gtgttggcgc tgatacatcc atggcaccaa tgaaatccag
    27361 ggctttatct attacagcag gcatattctt atccttaaaa ttaaccatgt tatcgataag
    27421 gctatatatg acctta
//
LOCUS       DAJTVJ010000050        27384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000050 DAJTVJ010000000
VERSION     DAJTVJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.050"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..543
                     /locus_tag="OO448_002046"
     CDS             <1..543
                     /locus_tag="OO448_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCR6337253.1"
                     /translation="VKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTA
                     VRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSP
                     REYLRQRACNGLSDNRCLEI"
     gene            555..1481
                     /locus_tag="OO448_002047"
                     /pseudo
     CDS             555..1481
                     /locus_tag="OO448_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1524..2384)
                     /gene="rsmI"
                     /locus_tag="OO448_002048"
     CDS             complement(1524..2384)
                     /gene="rsmI"
                     /locus_tag="OO448_002048"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6337254.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2448..4484
                     /gene="lpoA"
                     /locus_tag="OO448_002049"
     CDS             2448..4484
                     /gene="lpoA"
                     /locus_tag="OO448_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6337255.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            4442..4837
                     /locus_tag="OO448_002050"
     CDS             4442..4837
                     /locus_tag="OO448_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6337256.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4857..5447
                     /gene="diaA"
                     /locus_tag="OO448_002051"
     CDS             4857..5447
                     /gene="diaA"
                     /locus_tag="OO448_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6337257.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5457..6032
                     /gene="yraP"
                     /locus_tag="OO448_002052"
     CDS             5457..6032
                     /gene="yraP"
                     /locus_tag="OO448_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6337258.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6146..7186)
                     /locus_tag="OO448_002053"
     CDS             complement(6146..7186)
                     /locus_tag="OO448_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6337259.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7259..7894)
                     /locus_tag="OO448_002054"
     CDS             complement(7259..7894)
                     /locus_tag="OO448_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6337260.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8022..8540
                     /gene="yhbO"
                     /locus_tag="OO448_002055"
     CDS             8022..8540
                     /gene="yhbO"
                     /locus_tag="OO448_002055"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6337261.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8520..8963)
                     /locus_tag="OO448_002056"
     CDS             complement(8520..8963)
                     /locus_tag="OO448_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6337262.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9014..9316
                     /gene="yhbQ"
                     /locus_tag="OO448_002057"
     CDS             9014..9316
                     /gene="yhbQ"
                     /locus_tag="OO448_002057"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6337263.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9303..9806)
                     /locus_tag="OO448_002058"
     CDS             complement(9303..9806)
                     /locus_tag="OO448_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6337264.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9800..10324)
                     /locus_tag="OO448_002059"
     CDS             complement(9800..10324)
                     /locus_tag="OO448_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6337265.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10533..11528
                     /locus_tag="OO448_002060"
     CDS             10533..11528
                     /locus_tag="OO448_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6337266.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11537..12415
                     /locus_tag="OO448_002061"
     CDS             11537..12415
                     /locus_tag="OO448_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6337267.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12621..13628
                     /locus_tag="OO448_002062"
     CDS             12621..13628
                     /locus_tag="OO448_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR6337268.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13746..14990)
                     /gene="mtr"
                     /locus_tag="OO448_002063"
     CDS             complement(13746..14990)
                     /gene="mtr"
                     /locus_tag="OO448_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6337269.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15144..17033)
                     /gene="deaD"
                     /locus_tag="OO448_002064"
     CDS             complement(15144..17033)
                     /gene="deaD"
                     /locus_tag="OO448_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6337270.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17026..17106)
                     /gene="yrbN"
                     /locus_tag="OO448_002065"
     CDS             complement(17026..17106)
                     /gene="yrbN"
                     /locus_tag="OO448_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6337271.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17213..18097)
                     /gene="nlpI"
                     /locus_tag="OO448_002066"
     CDS             complement(17213..18097)
                     /gene="nlpI"
                     /locus_tag="OO448_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6337272.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18206..20341)
                     /gene="pnp"
                     /locus_tag="OO448_002067"
     CDS             complement(18206..20341)
                     /gene="pnp"
                     /locus_tag="OO448_002067"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6337273.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20588..20857)
                     /gene="rpsO"
                     /locus_tag="OO448_002068"
     CDS             complement(20588..20857)
                     /gene="rpsO"
                     /locus_tag="OO448_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6337274.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21006..21950)
                     /gene="truB"
                     /locus_tag="OO448_002069"
     CDS             complement(21006..21950)
                     /gene="truB"
                     /locus_tag="OO448_002069"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6337275.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21950..22351)
                     /gene="rbfA"
                     /locus_tag="OO448_002070"
     CDS             complement(21950..22351)
                     /gene="rbfA"
                     /locus_tag="OO448_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6337276.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22515..25187)
                     /gene="infB"
                     /locus_tag="OO448_002071"
     CDS             complement(22515..25187)
                     /gene="infB"
                     /locus_tag="OO448_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6337277.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25212..26699)
                     /gene="nusA"
                     /locus_tag="OO448_002072"
     CDS             complement(25212..26699)
                     /gene="nusA"
                     /locus_tag="OO448_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6337278.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26727..27179)
                     /gene="rimP"
                     /locus_tag="OO448_002073"
     CDS             complement(26727..27179)
                     /gene="rimP"
                     /locus_tag="OO448_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6337279.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         6498 a         7573 c         6922 g         6391 t
ORIGIN      
        1 gtgaaagaaa gcaatcagcg atggtgctct gacgggttcg agttctgctg tgataacgga
       61 gagagactgc gtgtcacgtt cgcgctggac tgctgtgatc gtgaggcact gcactgggcg
      121 gtgactaccg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgatct tccgtcgtct ccagtggagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acgccagttc gcccggatgt tgggacttga accgaagaac
      301 acggcggtgc ggagtccgga gagtaacgga atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtatcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cgctgggtta tcgctcgcca
      481 cgggaatatc tgcggcagcg ggcttgtaat gggttaagtg ataacagatg tctggaaata
      541 taggggcaaa tccatttacc tctgcaacgc taagtagcac taactggcaa tacgcctgtt
      601 cctgctctgc gggtaaggca gttaaacttg tctatatggt cagccccgta cttaccacca
      661 ctggacatca gacaggatat tacaaactca atgacagcct ggatattaaa accacattac
      721 aggcaaacga cattccagga ctcacaaccg accaggttgt ctctgttaac acccgattca
      781 cacagataaa aagcagcacc gtatattctg ctgcaaccca aacgggtgtt tgccagggtg
      841 acacgtctcg ttatggaccc gttaatattg gtgcaaatac cacctttacc ctgtatgtca
      901 ccaagccatt tctcggctcg atgaccattc cgaaaacgga tattgccgtc attaaaggcg
      961 cgtgggtcga tggaatggga agcccgtcta caggtgactt ccatgattta gtcaagttat
     1021 cgattcaggg aaatctcacc gccccacagt cgtgcaaaat taatcagggc gatgttatta
     1081 aggttaattt tggattcatc aatggtcaga agtttaccac ccgcaatgcc atgccagacg
     1141 gttttactcc agtagacttt gatatcactt atgactgtgg tgatacttca aagattaaaa
     1201 actcgttgca aatgcgcatc gacggtacaa ctggggtagt agaccagtac aacctggtcg
     1261 ccaggcgaag aagttcagac aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg
     1321 gcggagttgc aaatattcct tttcagaacg gtatccttcc cgttgatcct tccgggcatg
     1381 gcaccgtcaa tatgcgcgcc tggccagtta atctggtcgg tggtgagctg gaaacaggaa
     1441 aatttcaggg caccgccacc attaccgtca tggtgcggta aaagaagacg ttgcgttttg
     1501 attaacgcaa cgcttcggca ctgttacccc tgctgctcca gcgcatactt atacagcgca
     1561 tttttcttca cgccatgaat ttctgcggcc agcgccgccg cttttttcag cggcagttct
     1621 gcctgtagca gcgccagcgt gcgcagggca tcggcgggta agtcttcttc ctgtgcttta
     1681 tgaccttcga caatcagcac catttcgcct ttgcgacggt tttcatcttc ctttacccac
     1741 gccagcagct cgccaacggg cgcgccgtga atggtttccc aggttttggt cagctcacgc
     1801 gccagaacca cgtagcggga ttcgcctaat accgcaacga tatcttccag gctatctaac
     1861 agacggtggg tagattcata aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc
     1921 gcatcacggc ggccttttga tttggcaggt aaaaagcctt cgtaacagaa acggtcagag
     1981 ggtaaacccg cggcgcttaa cgcagtgata gcagcacacg gtccgggtag cggcaccaca
     2041 cgaatccccg cttcacggca ggtacgcacc agatggtagc caggatcgtt aattagcggc
     2101 gttccggcat cggaaaccag cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt
     2161 tcggcttttt gttgttcgtt atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa
     2221 tgttgcagca ataaaccggt gtgacgagta tcctcggcgg caatcagatc aacggcctgt
     2281 aatacctcta acgcacgctg ggtgatatcc gccagattgc cgattggcgt cggtacaatg
     2341 taaagctggc cctgagaatt atccgccgat tggtgttgtt tcattgtgtc gtccgtattg
     2401 ccgatttaat attgagcatt gcgtaaaaaa atatcactgg atacattatg gtaccctcaa
     2461 cattttctcg tttgaaagcc gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg
     2521 ccggttgtgg cacccatact cccgatcagt ccactgctta tatgcagggc acggcgcagg
     2581 ctgattctgc cttttatctt cagcagatgc agcaaagctc tgatgatacc aggatcaact
     2641 ggcaattact cgccattcgt gcactggtga aagaaggtaa aaccgggcag gcggttgagt
     2701 tgtttaacca actaccgcaa gaactgaacg attctcagcg tcgcgagaaa acactgctgg
     2761 cggtagagat taaactggcg cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa
     2821 tcacacctgc cgatttagaa caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg
     2881 ccagccaggg gcgtccttcc attgatttac tgcgcgcgtt aattgctcag gaaccgctgc
     2941 tcggcgcgaa agaaaaaaag cagaatattg atgccacctg gcaggcgctc tcctccatga
     3001 ctcaggaaca ggcgaatacg ctggtgatca acgccgacga aaatattctg caaggctggc
     3061 tggatctgca gcgcgtctgg tttgataacc gtaacgatcc cgacatgatg aaagccggga
     3121 tcgccgactg gcaaaaacgt tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt
     3181 tggttaacgt aaaagcgttt aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc
     3241 tgaatggcca ggcagcggta tttggtcgca ccattcagca aggctttgaa gcggcgaaaa
     3301 atatcggcac tcagccagtg gcggctcagg tagctgccgc acctgccgca gacgtagctg
     3361 aacaacctca gccgcaaacc gtggatggcg ttgccagccc ggcacaagcc tcggttagcg
     3421 atctgaccgg tgatcagcct gcggcccagc cggtgcctgt aagcgccccg gcgacaagca
     3481 ccgcagcggt aagcgcaccc gcaaatccat ccgcagagct gaaaatctac gatacctcat
     3541 cacaaccact tagccagatc ttaagccagg ttcagcagga tggcgcgagt attgtggttg
     3601 gtccgttgct gaaaaataac gttgaagagt tgctgaagag caacaccccg ctgaacgtgc
     3661 tggcactgaa ccagccagag aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt
     3721 cacctgaaga cgaagcgcgc gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc
     3781 cgctggtgct gatcccacgc agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag
     3841 agtggcagaa actgggcggc ggcaccgttc tgcaacaaaa atttggttcc accagcgaat
     3901 tacgcgcggg tgttaacggc ggttctggta tcgctttaac gggtaccccg attactccca
     3961 gagcgacaac cgactccggc atgacgacca acaatccaac gctgcaaacc acgccaaccg
     4021 atgaccagtt caccaataat ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg
     4081 gtgaaatcgc ttttatcaaa ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa
     4141 tgctgtacgc cagctcccgc agcgcacaag gcaccgcagg cccggatttc cgtctggaga
     4201 tggaaggttt gcagtacagc gaaatcccga tgctggcggg cggtaatctg ccgttaatgc
     4261 agcaggcact cagcgcggtg aataacgatt attcactggc tcgcatgtat gcgatgggcg
     4321 tcgatgcctg gtcgctggca aatcatttct cacaaatgcg ccaggttcag ggttttgaaa
     4381 tcaacggtaa taccggaagc ctgacggcaa acccggattg cgtgattaac aggaagttat
     4441 catggctaca gtaccaacaa ggtcaggtag tccccgtcag ttaaccacca aacagaccgg
     4501 cgatgcgtgg gaagcacaag cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc
     4561 cgctaacgtg aacgagcgtg gcggcgagat cgatctgata atgcgtgaag gccggaccac
     4621 cgtttttgtc gaggtacgct atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt
     4681 gacccgcagc aaacaacaca aattattaca gactgcccgc ttgtggctcg cgcgtcataa
     4741 tgggagtttt gatactgtgg attgccggtt cgatgtggta gccttcaccg ggaatgaggt
     4801 tgagtggatt aaggatgcct ttaatgacca ctcataatta aggtttaagg attagcgtgc
     4861 aagaaagaat taaagcttgc ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg
     4921 cgcttccgga tgccatctcc cgtgcagcca tgacgctggt tcagtctctg ctcaatggca
     4981 acaaaatcct ctgttgtggt aatggaactt ccgctgccaa tgcacagcat tttgctgcca
     5041 gcatgatcaa ccgtttcgaa acggagcggc ccagcttacc tgccattgca ctaaatactg
     5101 ataatgttgt cttaacggcg attgccaacg atcgcttaca tgatgaagtg tatgcaaaac
     5161 aggtgcgggc gctgggtcat gcgggagatg tattgttagc catttccacc cgtggcaaca
     5221 gccgcgatat tgttaaagca gttgaagccg ccgttacgcg tgatatgacc attgtggcat
     5281 tgaccggcta tgacggcggc gagcttgcag gtttgttagg gccacaggat gtggagatcc
     5341 gcattccttc gcatcgtagt gctcgcattc aggaaatgca tatgctgacg gtaaattgcc
     5401 tgtgcgatct gatcgataac acgcttttcc ctcaccagga tgattaagga gaatacatga
     5461 aggcattatc gccaatcgca gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg
     5521 ctgccgtagt gggtaccgct gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg
     5581 gcacccaggt ggacgatggt accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg
     5641 aacagattaa gaaagaaacc cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg
     5701 ttgggcagtc accaaacgct gaactttcgg ctcgcgccaa acagattgct atgggcgtag
     5761 acggtgccaa cgaagtgtat aacgagattc gtcagggcca gccgattggt ctgggcgaag
     5821 catctaacga tacgtggatc accaccaaag tgcgttcgca gctcttaacc agcgacctgg
     5881 tgaaatcgtc caacgtgaaa gtgaccaccg aaaacggtga agtgttcctg atggggctgg
     5941 tgactgaacg tgaagcgaaa gcggcggcag atattgccag ccgggtgagc ggcgtaaagc
     6001 gggtaaccac agcgtttacg tttattaaat agcagcccct tgtaatgcct gatgcgacgc
     6061 ttgccgcgtc ttatcaggcc tacaacgatg caaaccgtag gtcggataag gcgtttacgc
     6121 cgcatccgac atgttttccc tcaaatcaga ataacagcgc cagcccgccg acaatcacgc
     6181 ctgacactgc caccatcgcc cccgtcagcc ataaggcttt cgccgggaac gctttgcgca
     6241 gcataatcag tgacggcaaa ctcactgccg ggagcgtcat caacaatgcc agcgccggag
     6301 cggttcccat acctgccagc atcatcgttt gtacaatcgg aatttctgct gccgtgggaa
     6361 tgacaaacaa gcatcctgct accgccatcg ccaccaccca catcaggctg ttatcgacag
     6421 caccatcggc atgggggaat aaccagacgc gtgcggcacc caacaccagt actgcaagga
     6481 tgtaaaccgg gatcgtactc cagaaaagtg tccatagcgc cctgccccag cggctaaaaa
     6541 aaccgctctg tgcttccggt atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac
     6601 gcacccattt ttgcaccagc gtcgcaatca gcaacaccat caccagcccg gccaccagac
     6661 gaatcgccgc aaaaccccag ccgaggacaa accccataaa caccagcgtc gccgggttta
     6721 acaccggatt gcccatccag aatgccagcg caccgcccat cgatacctgt tggcgacgca
     6781 ttcccgccgc gaccggagcc gcacagcagg tacacatcat gcccggcagc gaaaacagcg
     6841 ttcccagcag cgtgccgcga aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg
     6901 ggatcagcac ctgaatcaac gaaccgagga tcacccccag caccgccgct ttccataccg
     6961 cgaggaaata gatcatcgcg taatccaacg ccgcctgcca cggattagca tccgcctgcg
     7021 caaggataga tttaccgata ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag
     7081 gttgccattt cacataccag agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc
     7141 accactgaat gggcgttgcc gcctgagatg aagactgacc agtcatagca ttccccagga
     7201 ataagttatg ataattgagc gcgtgaatat tacgctcact atcaattctt gggaataatt
     7261 attcagctct tttgcgtaat tctgaagagc ttaaaatagt cacgccttca tgctcaggcc
     7321 gcatcgcctc tgccagcatg acccgcgcca cgtctctcgc atcaatggat ttccagttac
     7381 cgggcagtaa acgaaacagc ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat
     7441 cgcccagcaa catcgacggg cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg
     7501 cttcttccat ctcccctttg acgcggttat agaaaaacgg cgagtgggca ttggcaccca
     7561 tagcactgac caccagcata tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca
     7621 ctaccagcgt gtaatcggca tgaataaacg cctctttgct ccccgcttct cgccgcgtgg
     7681 tgccgagaca acaaaacaca atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca
     7741 gttgcggatc atggggatta aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg
     7801 cggctatggc gttaactttc ggttcgttaa tcaacatccg cagcaggtga ccgcccacca
     7861 gccccgttgc gcctgtaatc agtacctgac tcatcttcgc tcctttacag aattgtctgc
     7921 cttgcgctcc acggctcatg caccaggctt aatagaccat gaggtaatta tccccgattg
     7981 tgtgaaattc gcctcatcca atgcaacaac gcggaggaag catgagtaag aaaattgccg
     8041 ttttaatcag tgatgaattt gaggattcag aatttacttc acccgcagac gagttccgta
     8101 aagccggaca cgaagtgatt accattgaaa aacaagcggg taaaacggtg aaaggcaaaa
     8161 aaggagaagc cagcgtgacc atcgataaat ccatcgatga agtgacgcca gcggagtttg
     8221 atgccctgct gctaccgggt ggtcattcac cggattatct gcgtggcgac aaccgttttg
     8281 tcacctttac ccgtgatttt gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc
     8341 cgcagctgct gatcagcgcc gatgtgattc gggggcgcaa actgaccgca gttaaaccga
     8401 tcattattga tgttaaaaat gcgggcgcgg aattttacga tcaggaagtc gtggttgata
     8461 aagatcagct ggttaccagc cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt
     8521 tacgcctgct cggtgcctga gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg
     8581 ttgtcggtga atttgatttc atcgagccgg atttcccaca ccggggccga cagcattctg
     8641 gcaaccggaa agcgacgatt gtacgcctgg cgcgcgaggt cgctttcctc accttccagc
     8701 ctgcggatct cacctttaaa ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg
     8761 tttaccgttc cggcaaccgc agcctgcggc ccactcatct gcgcgtggcg cgttttttct
     8821 tccgtcaaaa tgtagaaggc gactttctgc gcatcaaaca gataaaaagc attagcgcac
     8881 cacagttccc cttcctgctg cacacaccag gtgacaacat gttgttttgc caaccaacgg
     8941 ctgatggtga tgagtgtttc cattgctgct ctcttttata ctgtgggcct gaccttaaca
     9001 tgcgacgcag acgatgacac cctggtttct ttacttgatc cgtaccgccg acaataagct
     9061 ttataccggg atcaccacgg atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg
     9121 ggcgaaagca ctgcgcggga aaggagaact gacgctggcg ttttctgcgc cagtgggcga
     9181 tcgttcgctg gcgttacggg cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga
     9241 gcgtctggta gcggaaggcg cagggtttgc agagctgtta agcagtctgc aaaccccgga
     9301 gattaaaagc gattgaaatg ctcgtgatac tcaaccagac cggttacgcc attcagcgcg
     9361 tcatccgcta aacgatgtac ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga
     9421 tcgtgatgcg ccgccagttc aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc
     9481 accactgcgg catagccgaa ctcattaagc gaatcgagtc cttcatagac cagttggcgt
     9541 gccagcccct gtccacggta tttttcatca accgccaacg gtgccatgcc gacccattgc
     9601 aggtcttcgc cctgcacatc aaccggacta aatgccacat agccaatgac ctgaccttcg
     9661 tcatctgtcg ccaccagccc cagcgtcaga aagccatctt cacgcagatc gtgaaccagc
     9721 ttcgcttccg catcactttc gaatgagcga cgcagcaggg catcaatacc cggcgcatca
     9781 atgggaattt ctactcgaat tagcatggtt cacctaccga tgtctgtttg gtttcaggcg
     9841 cggttttcat tcccgcctca acaaaatccg ccagttgcag cagcatcatg cgtaaggctt
     9901 tcggcatttg ctccagttca atggcgtcca tcaggttttt cacatacagc cccagctccg
     9961 tatcgccttc aatcaccagc cgacgctgga agaagagcgt atccggatct tgtttacgcg
    10021 ccgcaatcat cagcagatcg ctggcgtcgg cactaaaact cacatcagct tgcgcgttct
    10081 ggctgaccac cagtttgcca ttcaccaccg aggtaaacca ttgcaggtca atatcacgca
    10141 catgaatact taaccagcgg ccttcaagaa actccagctc accatcatcc agcgcctgac
    10201 ggaattgcca gcttaagacc tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt
    10261 ttaccggtac actcaacaga gatggcccca aatgcacaat acgggaacgc agtttatcca
    10321 acacgagttt tactccctgt ttcaacaatc atcctatttt gccatatcag aaaaataaca
    10381 tagcggtata aatcaacaat tccatatgaa attgctgcta ccaccaatac aactttaact
    10441 gccttaaatc aaaaattgtc gcagcaaggt taactaaaat cccagttcgt taacattttt
    10501 gcgttttgaa agcgcaacct tcaggaaaaa ttatggagct gctctgccct gccggaaatc
    10561 tcccggcgct taaggcggcc atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag
    10621 atgatactaa tgcccgtcac ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag
    10681 cggtgagttt tgtccatcaa catcgccgca aacttcacat cgcgattaac acttttgcgc
    10741 atccggacgg ttacgcccgt tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg
    10801 acgcgctgat cctcgccgac ctcgccatgc tggagtacgc cgccgagcgt tacccgcata
    10861 ttgagcgtca tgtgtcagtg caggcttcgg cgaccaatga agaggcgatt aacttttatc
    10921 atcgccattt tgatgttgcc cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga
    10981 aacagctggc acgggtcaca cctgtcccgc tggaagtctt tgcttttggc agcctgtgca
    11041 ttatgtcgga aggtcgttgc tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg
    11101 tgggcgcgtg ttctccggcc cgtttcgtgc gctggcaaca aacgccgcag gggctggaat
    11161 cccgcctgaa cgaagtgctg atcgaccgtt atcaggatgg cgaaaacgca ggttatccga
    11221 cgctgtgtaa agggcgttat ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa
    11281 ccagtctcaa taccctggaa ctgctgccgg agttaatggc ggcgaatatt gcttcggtga
    11341 aaattgaagg ccgccagcgt agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc
    11401 aggctatcga ccgttgtaag gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg
    11461 agacgctcgg gtcgatgtcc gaaggcacgc agaccaccct tggcgcgtat caccgtaaat
    11521 ggcagtgaga agagcaatga aatattcctt agggccagtg ctgtggtact ggccaaaaga
    11581 ggcgctggaa gaattttatc agcaggccgc caccagcagc gccgacgtga tttatcttgg
    11641 tgaagcggta tgcagcaagc gccgggcaac caaagttggc gactggctgg agatggcaaa
    11701 atcgctcgcc ggaagcggga agcagatagt gctctccacg ctggcgctgg tgcaggcatc
    11761 ctctgaactg ggcgaactga aacgctatgt tgagaacggt gagtttctga ttgaagccag
    11821 cgatctcggc gtggtgaata tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc
    11881 gctgaactgc tacaacgcgg tgacgctgaa aatattgctc aaacagggca tgatgcgctg
    11941 gtgtatgccg gtggagcttt cccgcgactg gctggtgaat ctgcttaatc agtgcgatga
    12001 gttggggatt cgcaaccagt ttgaagtgga agtcctgagc tacggtcatc taccgctggc
    12061 ctactccgcc cgctgcttta ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac
    12121 ctgctgcatt aagtatccga acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt
    12181 tgtactcaat ggcattcaga ccatgagcgg ctacgtttac aacctcggta acgagctgac
    12241 atccatgcag ggattggttg atgtggtccg cctgtcaccg cagggtactg acactttcgc
    12301 gatgctcgac gccttccgcg ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa
    12361 cagcgactgt aacggctact ggcggcggct ggctgggctg gagctgcaag cctaagtaaa
    12421 tatgctcgtc atacttcaag ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt
    12481 acttatgtaa gctcctgggg attcactctc ttgtcgcctt cctgcaactc gaattattta
    12541 gagcacagct cactttgtta acaactttaa ctactcttta atgcagtatt aaagattaat
    12601 cggtaacaaa gtgagctgtt atgactgata aaaccattgc gttttcgcta ctcgatctgg
    12661 cccccattcc cgaaggttct tcagcgcgag aagcattctc ccactctctc gatctcgccc
    12721 gtctggctga aaagcgcggc tatcatcgct actggctggc agaacaccac aatatgactg
    12781 gcattgccag tgctgccacg tcggtattga ttggctatct ggcggcgaat accaccacgc
    12841 tgcatctggg gtctggcggc gtgatgttgc ctaaccactc accgttggtc attgccgaac
    12901 agttcggcac gctcaataca ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc
    12961 caggtagtga ccaacggaca atgatggcgc tgcgtcgcca tatgagcggc gatattgata
    13021 atttcccccg cgatgtcgcg gagctggtgg actggtttga cgcccgcgat cccaatccgc
    13081 atgtgcgccc ggtaccaggc tatggcgaga aaatccccgt gtggttgtta ggctccagcc
    13141 tttacagcgc gcaactggcg gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg
    13201 cgccggatat gttgttccag gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac
    13261 gactggaaaa accatacgcg atggtgtgca tcaatattat cgccgccgac agcaaccgcg
    13321 acgctgaatt tctatttacc tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa
    13381 ccggacaact gccgccgccg attcaaaata tggatcagtt ctggtcaccg tctgagcagt
    13441 atggcgtgca gcaggcgctg agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg
    13501 gcttgcagtc gatcctgcgc gaaaccgacg ccgatgaaat tatggttaac gggcagattt
    13561 tcgaccacca ggcgcgcctg cattcgtttg aactggcgat ggatgtgaag gaagagttgt
    13621 tgggatagtg tgtcttaacg cgggaagcct tatccgagct ggcaacgctg tcctacgtag
    13681 acctgataag cgaagcgcat caggcattgt gtaggcagca gaaatgtcgg ataaggcacc
    13741 gctgattact gatacaccgg cagcaaatta aagctcgata agatatggac cagtgcgttg
    13801 ccaacgccaa acaccagaat cagcgtaatc atcggcttgc cgccccagac acggaatttc
    13861 gggctgccaa agcgtttacg cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag
    13921 atggtagctg ctaaaccagc ataaccaatg gcgtacagga atccgttcgg gaacagtaga
    13981 ccacccacaa caggcggggc aaaggtcagc aatgccgttt tcaagcgccc cagggctgag
    14041 tcatcgaaac caaacagatc tgccagatag tcaaacaaac ccagcgttac gccaaggaac
    14101 gaactcgcta ccgcaaagtt tgagaacacg accagcagaa ggtccaggct acggctgttc
    14161 agcacgccgc ttaatgcctg taccagcaca tcaatattac cgcccttctc tgcaataccg
    14221 ataaactccg gacgcgggat gttacccatc gtcgccagca accagatggt atacagcgcc
    14281 agtgccatca gcgtaccgta caccaggcat ttcacgatgg ttttcggatc tttgccgtaa
    14341 tacttcatca ggctcggcac gttaccgtga taaccaaacg atgccagaca gaacggcagg
    14401 gtcatcagca aatacggcgc ataagatgca ttgctttcgg cgacgttgaa caatgtcgca
    14461 ggctgcacat gccccagtag gctaccgaag gtgaggaaga aggtaatgac tttcgccccc
    14521 agtacaatcg ccgtcatgcg gctgacggcc ttagtactca accacaccac aaacgctacc
    14581 agcagtgcaa aaccaaaacc cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag
    14641 gtgtgatgca gaattgaacc actggcagaa atataggcat aggtcaggat atagagcaca
    14701 aaggcaatgg aaataccgtt gaccacgttc cagcctttgc ccagcaaatc tttggtgatg
    14761 gtgtcaaaac tcgaaccgat tcggtaattc aggttagctt ccagaatcat caagccggaa
    14821 tgcagcatac agaaccaggt aaagatcagc gccgccattg accagaaaaa ccacgccccg
    14881 gacatgacca ctggcagaga aaacatccct gcgccaataa tggtgccgcc gataatcacc
    14941 acgccgccaa gcagcgacgg tgacgtttgg gtggtggtta gtgttgccat gagagcttct
    15001 ctccagtgaa aaatagtgcg actgcgttgt tatgcattgc actgtaccag tacacgagta
    15061 caaaagacag aaaaaaagcc ccgatggtaa aaatcggggc tgtatatatt attttacaga
    15121 ttgtgttcgc tgttcagcga tgattacgca tcaccaccga aacgacgacg accggtagag
    15181 tcatcacgac gcggagcgcg gccttcacga cgttcgccgc taaaacgacg accatcacca
    15241 cggccacctt cacggcgttc accgctgaag ttacgaccgc cttcacgacg ttcgccaccg
    15301 aaaccacgac caccgccacg acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac
    15361 tgcatgttca tcggcttgtt gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc
    15421 ggcatacctt tcggcagttc gatggtggag tgagaagcaa acagcttgat gttaccaatg
    15481 taacggctac tgatgtcgcc ttcgttagca atcgcaccaa cgatatgacg aacttcaaca
    15541 ccatcatcgc ggcccacttc aatgcggtac agctgcatat cgccaacatc acgacgttca
    15601 cgacgcggac gatcttcacg gtcaccacgc gggccacggt cgttacgatc gcgcggacca
    15661 cggtcatcac ggtcacggaa ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc
    15721 agagtacgtt caccctgtgc cattttcagc agtgccgcag ccagagtttc gagatccagc
    15781 tcttcacctt ctgcagtcgg ctgaattttg ctcagcagtg cgcggtattg atccagatcg
    15841 ctgctttcca gctgctgctg tactttagcg gcgaattttt ccagacggcg tttgcccagc
    15901 agttctgcgt tcggcagttc tacttccgga atagtcagct tcatagtacg ttcaatgttg
    15961 cgcagcagac gacgctcgcg gttctcaacg aacagcagcg cgcggccagc acgacccgca
    16021 cgaccggtac gaccgatacg gtgaacgtaa gactcagaat ccatcgggat atcgtagtta
    16081 actaccaggc tgatacgctc aacgtccagg ccacgggctg caacgtcggt cgcaatcagg
    16141 atgtccagac gaccatcttt caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg
    16201 tcaccgttca gcgcggcgct gttgtagccg ttacgctcaa gagcttcagc cacttccaga
    16261 gtcgcgtttt tggtacgaac gaagataatc gccgcatcaa aatcttccgc ttccaggaaa
    16321 cgtaccagtg cttcgttttt gcgcataccc cagacagtcc agtagctctg gctgatgtca
    16381 ggacgggtag tcacgctgga ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg
    16441 gtaatgcgac gaatcgcttc cggcatggtt gcagagaaca gagcggtctg atgaccttcc
    16501 gggatctgcg ccataatggt ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca
    16561 gcttcatcca gaaccagacc gctcagttta gagaggtcca gagtgccacg tttcaggtgg
    16621 tccagcagac ggcccggagt accgacaacg atctgcggcc cctgacgcag ggcgcgtaat
    16681 tgcacgtcat aacgctggcc gccgtacaga gcaaccacat ttacgccgcg catgtgttta
    16741 gagaaatccg tcattgcttc agcaacctgt accgccagtt cgcgggtcgg tgccagcacc
    16801 agaatctgtg gtgctttcag ctcaggatca agattctgca acagaggtaa agagaatgct
    16861 gcagtttttc cgctccccgt ctgggccata cccagaacgt cgcggccatt cagcagatgt
    16921 ggaatacact ctgcctgaat tggagatggt ttttcgtaac ccagatcgtt aagggcttca
    16981 aggataggag ccttcaggcc cagatctgca aaagtggttt cgaattcagc catgtagtac
    17041 gtgtgcctca aaattaatgg cggccagtct acataactca tcatgaaatt gatcagcaat
    17101 tttcattgaa aagtgtgaac cggctcaaag taggtgtatt aacgaacaac aacgccctca
    17161 cccgttaagg tgatggcaat caaaaaagat tacgggctga tgtgtacgtc agctattgct
    17221 ggtccgattc tgccaggtca tcttggtcct ggcccaggag cgataattcc aacaatgcgt
    17281 atcggtgctc aacaaagtta tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg
    17341 cgctgtccaa atcccccaga cttaggtagt acttacctaa atagaagttg gtttcactga
    17401 gatgctcagc gagcgaggtg ttatccgttg cgtccgcctt gagcctttcc attaacgttt
    17461 gttcgctaat gttgcccagg tagaactcga caatgttcca tccccactgt tccttatccg
    17521 atttttcgaa gtgctgtttc aacacttctt tagcctgctt ctcatcgagc ttctgctcgg
    17581 cgagataaag ccacagacta cggaaaggat cattgggatc gtcttgataa aacgccagca
    17641 gatcatcttg cgctaacttg tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt
    17701 gcgcgtagtt gtaagttgga tcaagctcaa gtacagaatc aaacgcttca taggcagcat
    17761 caaaattgcc tgcctgcgtt aaatatatgc ctaagtaatt gaatacttca ggcatatccg
    17821 gtcggattgc cagcgcttgc gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat
    17881 catacaacac tccgcgctca tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac
    17941 tggcaaggat ttgttccata cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg
    18001 gtaccgcgag gacttcactt ttacgccagg aagtattact gcatcctgca agcgtaagtg
    18061 ctgtcgcaac gaaacaccag cgcaaaaaag gcttcatttc ccactcccga agaccacggt
    18121 tgaatgaacg tcctgttccc ggttgctaac aaggcgtcct gcccggttaa aagccccccg
    18181 ccgcagcgga gggcaaatgg caaccttact cgccctgttc agcagccgga gcttccggtg
    18241 ctgcagcagg ttgagactgc tcagtcgctt ctttaatgct cagacggata cggccctggc
    18301 gatcaacttc tagaactttc accggtactt cctgacccat ctgcaggtaa tcggtcactt
    18361 tctcaacgcg tttgtcagcg atttgagaga tgtggaccag accttcttta ccgccgccga
    18421 tggcaacaaa tgcgccaaag tcaacgatac gggtcacttt accagtgtag acgcggccca
    18481 cttcgatttc tgcagtgatc tcttcgatac gacgaatagc atgtttcgct ttctcgccgt
    18541 cggtcgctgc gatcttcaca gtaccgtcat cttcgatttc gatggtagtg ccagtttctt
    18601 cggtcagggc acggataaca gagccgcctt taccgataac gtctttgatc ttatccgggt
    18661 tgatcttgat ggtatggata cgcggtgcga actcagagat atcgccacgc ggcgcgttga
    18721 tcgcctgttc cattacgccc aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa
    18781 cctgcatgat ctctttggtg ataccttcaa ttttgatatc catctgcagt gcagagatac
    18841 cgtcgcggga acctgcaact ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa
    18901 tgtcagacag tacaacgtag ttgtcgcctt ctttcaccag acccattgcg atacccgcaa
    18961 cggcagcttt gattggcaca cctgcgtcca tcagcgccag agacgcgccg cacacggaag
    19021 ccatagaaga agaaccgttg gattcggtga tttcagacac tacacgtacg gtgtacggga
    19081 atttgtccat atccggcatg actgccagca cgccgcgctt cgccagacga ccgtgaccaa
    19141 tttcacgacg cttcggagaa ccgaccatgc cggtttcgcc tacggagtac ggagggaagt
    19201 tgtagtggaa caggaaggta tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt
    19261 cacgagcggt acccagcgtt gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg
    19321 cagaaccgtg agtacgcggc agcacgccag tacgcacatc cagaccacgg atcatatctt
    19381 tttcacgacc gtcgatacgc ggttcgcctg ccagtacgcg gctacgaaca acgtttttct
    19441 cgatagcgtg cagaatttca cccagttcgt tttcgtccag ggtttcgtct tcagcaagca
    19501 gcgtcgcgat ggtttcagat ttgatgacat caacctgcgc ataacgctct tgtttgtcgg
    19561 tgatgcggta agcatcactc agacgagctt cagccagtgc agcaacgcgc gcgttcagcg
    19621 cttcgtttac cggctccggc tgccagtccc aacgcggttt accggcttct ttcactagtt
    19681 cattgatgtt ctgaataaca acctgctgtt gttcatgacc gaacactact gcgcccagca
    19741 tctgatcttc gctcagcagt tcagcttcag attcaaccat cagtacagcg gcttcagtac
    19801 cggcaacaac cagatccagt ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt
    19861 actggtcatt gatgtaacct acgcgggcag caccaatcgg gccattgaac ggaataccag
    19921 acagagacag tgctgcggaa gcaccaatca tcgcgacgat atccgggtta acctgcgggt
    19981 taacagaaac cacggtggcg ataacctgaa cttcgttgac gaagccttcc gggaacagcg
    20041 ggcgaatcgg gcggtcaatc agacgcgcga tcagggtttc gccttcgctt ggacgacctt
    20101 cacgacggaa gaagctaccc gggatacgac cagcagcgta ggtacgctcc tgatagttaa
    20161 cggtcagtgg gaagaagtcc tgacctggtt tggctttttt ctgtccaaca acggtaacga
    20221 ataccgcggt gtcatccatg ctaaccataa cagcggcagt agcctgacga gccatcatgc
    20281 cggtttccag agtcacggtg tgttggccgt actggaattt acgaacgatc ggattaagca
    20341 atgtaatatc ctttctcttt cttagacagt accttacggc actggtgtta atacccgatc
    20401 ttctgcgcat cctcgcgact aatgacaacc ctaacccagc tctatgtggg taaagcctct
    20461 cattagccgc gcgaacctct gcaacggaag atcattcata gcaacaatac attagtttcc
    20521 agtgaattgc tgccgtcagc ttgaaaaaag gggccactca ggcccccttt tctgaaactc
    20581 gcaagaatta gcgacgcaga cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt
    20641 tacgtttcag gtagtcgagc agtttacgac gctgagaaac catgcgcagc agaccacgac
    20701 ggctgtggtg atcttttttg tgctctgcaa agtggccctg caggtggttg atctgtgcag
    20761 tcagcagtgc tacctgaact tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag
    20821 aaacgatttt agctgttgct tcagtactta gagacatttt aaaactccaa agtatataga
    20881 atgaaaggac gccgatctct aattcagcga tcccagtgta cgttacgcaa agtgttaaac
    20941 aatttacgcg acgttaagcg gcagtattct actcgtagcg acctgttatc gcaagacggt
    21001 taacattacg ccgggtattc aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca
    21061 atttcgccca taccgataaa cttgccgttc tcaccttctg taacgcgaac cagtccttcc
    21121 agcggcgcac cggatgtacg aactgggtta ccatttttga agtaaacaga agacgttaac
    21181 ggaagattca ccaccgggta gtccgaagct ggactgtcca ttggcatcag taatggatca
    21241 agtaactccg cagccggaat atcctgctgt tcggcttgtt cgaccagttc acgcagatgt
    21301 tccagggtca ccatccgttc aaccggatat ttacttaccg ccagacgacg caggtaaata
    21361 acatgcgcgc cacaaccgag tttttcaccc aggtcatcaa tgatggtgcg gatataagtg
    21421 ccttttgagc agtgaatttc cagctccagc tcattgcctt cgtggcgaat aaacagcaat
    21481 tcataaacgg taatcgggcg cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg
    21541 tacagttttt taccctgata tttcagtgct gaatacatcg aagggatctg ttcgatatcg
    21601 ccacggaaag tatccagcgc cgccgccagt tgctctgcgc taaaggttac cggacgttct
    21661 tcaacgattt gtccgtcggc atcagaagta tcggtacgct gtccaagacg cgcaatgacc
    21721 cgatagcgtt tgtcggagtc cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa
    21781 attggcaaca tgcctgttgc cagcgggtcc agcgcaccgg tatgcccggc acggttggcg
    21841 ttataaatcc gtttcacttt ttgcagcgca tcattgctgg acataccctg cggtttatcc
    21901 agcaacaaaa cgccgttaat gtcgcgaccg cgacgacgag gacgactcat tagtcctcct
    21961 tgctgtcgtc cgggttaaca cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt
    22021 ttgacatgcg catcccttca accagagagt tgtcgtagaa gaaggtcagt tccggcacga
    22081 tacgcaggcg catcgctttc cccagcaggc tgcggatgaa accagaagct tcttgcaacg
    22141 ctttgatgcc cgctttaacc gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt
    22201 tggcatacgc caggtcgcga gacatttcga caccggaaac ggtggtcatc atgcccaggc
    22261 gaggatcttt aatttcacgc tgcaggatga gagcgatctc tttttgcatt tcctgcgcta
    22321 cgcgctgcgg gcgaccaaat tctttcgcca taataaattc tcctgacaaa aaaggggctg
    22381 ttagcccctt tttaaaatta atttcaggtg gaagggctgt tcacgttggc ctgataagac
    22441 gcgccagcgt cacatcaggc aatccatgcc ggatgcggcg taaacgcctt atcccgcatg
    22501 gaaccctaaa aaccttaagc gatggtacgt tggatctcga tgatttcgaa tacttcgatc
    22561 acatcgccag tgcggacgtc gttgtagttc ttaacgccga taccacattc cataccgtta
    22621 cggacttcgt taacgtcatc tttgaagcgg cgcagggact ccagctcgcc ttcgtagata
    22681 accacgttgt cacgcagaac gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc
    22741 atacagcctg cgatagcacc aaatttcggc gatttgaaca cgtcacgaac ttccgccaga
    22801 ccgataatct gctgtttcag ttccggagac agcataccgc tcatcgccgc tttcacttcg
    22861 tcaatcaggt tatagatgac ggagtagtaa cgcagatcca ggctttccgc ttcaatcact
    22921 ttacgtgcag aggcatcagc acgtacgttg aagccaacca ggatggcgtt ggacgccgca
    22981 gccagggtgg cgtcggtttc ggtgatacca cctacgccag aaccgatgat cttcacttta
    23041 acttcgtcag tagacagttt cagcaaggag tcggagatcg cttccacaga accctgtacg
    23101 tctgccttca ggacgatgtt cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg
    23161 ttctcgagtt tagatttctg ctgacgcgcc agtttaactt cgcggaattt accctgacga
    23221 tacagtgcaa cttcacgcgc tttcttctcg tcacgtacaa cggtaacttc atcacccgca
    23281 gccggtacgc cggacaggcc gaggatttcc accggaatgg acggacccgc ttccagcact
    23341 tcctgaccca gttcgttacg catcgcacga acacgaccgt attcgaagcc acacagaacg
    23401 atatcgccct tgtgcagagt accttcacgt accagaacgg tagcaaccgg accacgacct
    23461 ttatcgagga aggattcgat aaccgcaccg ctcgccatac ctttacgtac cgctttcagc
    23521 tccagaactt ccgcctgcag caggatagcg tccagcagtt catcgatgcc ggtacccgct
    23581 ttcgcagata cgtgtacgaa ctggctttca ccgccccact cttccggcag gatgccatac
    23641 tgggagagtt cgttcttaac acgatccgga tcggcttctg gtttatcaat cttgttcact
    23701 gcaaccacca ccggtacctg cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc
    23761 atcacgccgt cgtcggcagc aacaaccagg actacgatgt ccgttgcctg cgcaccacgt
    23821 gcacgcattg aagtaaacgc ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt
    23881 tcagtttcaa cgtggtatgc accaatgtgc tgggtaatgc cgcccgcttc gccagaggca
    23941 actttcgttg aacgaatgta gtccagcaga gaggttttac cgtggtcaac gtgacccatg
    24001 atggtcacaa ccggcgcgcg cggttcagcc gcagcacccg tgtcacggtc gctcattacc
    24061 gcctcttcca gctcgttttc acgacgcagg ataactttgt ggcccatctc ttcagcaacc
    24121 agctgtgcgg tttcctgatc gataacctgg ttgatggttg ccattgcgcc cagtttcatc
    24181 atcgctttga tgacctgaga gcctttaacc gccatcttgt tcgccagttc gccaacggtg
    24241 atggtttcgc cgatcacaac gtcacggtta acggcctgag ctggcttctg gaagccttgc
    24301 tgcagggaag aacctttacg ttttccgcct ttaccgccac gtactgctgc gcgtgcttct
    24361 tcacgatcag cttttgattc agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg
    24421 ttacgaccac ggccacgacc gccttcgact tcacgatcgc tttcgtcttc tgcctggcga
    24481 gcatgttgag aagtagtgac gtgataatcg ctggaatctt cagtcggttc cgcgttatca
    24541 gtccatttgt tttcttccgc catacgacgt gcttcttcag caacgcgacg tgcttcttct
    24601 tcgagtttac gatgcgcttc ttcttcggct ttacgcttga gctctgcagc ttcctgctca
    24661 cggcgggctt tttcagcctg ggcgttttta gtcatatcgt cttgttgatt gctcactttg
    24721 tctttttccg cagcttcacg tttcgcttgt tcagcagctt cacgcttagc ttgttctgca
    24781 gcctcacgtt cagctttttg ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg
    24841 gcttgctctt ccgcttcacg ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc
    24901 ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga tttgtaccga tttgcttttt
    24961 ccaccggtac caggaatgtt aagggtgctg cgtgttttac gttgcagcgt caatttgtcc
    25021 gggcctgaat ttttctgatt caggtggtca atcaaagtct gtttctcttg tgcagacaca
    25081 gagtcgtcag cagacttccg gatacctgca tcagcaaatt gctgtaccag gcgttccacg
    25141 gaggtctgtc gctctgcggc cagcgtttta atcgttacat ctgtcatgct gttccttcct
    25201 gctacagttt attacgcttc gtcaccgaac cagcaaatat tacgggcagc cataatcagt
    25261 gctccggcct tttcgtcggt caacccttcg atatcagcca gatcatcaat gccctgttcg
    25321 gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa atcacgatct
    25381 accccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct ttcttcctgg
    25441 gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc aacggtcggc
    25501 tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccag ttcttccagc
    25561 gtcgagaagc cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg
    25621 gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg
    25681 gtcatcacgt tgagttccca accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta
    25741 cggccaatcg cctgcgccag gttaccggct tcaacggcga tatccatggt gtgtttatct
    25801 tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc
    25861 gggttatcat cccacaggac gatatcgata cgctcgccac ccagttcagt agacaccgcc
    25921 tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg tttatcgttg
    25981 gttttcaccg cgattttcgc acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc
    26041 acttcttcgc cgatttctgg cacttcaata cggaacagtt cgatcagcat ttccggcttg
    26101 gaacgagtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata aagcacgcca
    26161 cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcacg caggatcacg
    26221 gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt tttcaccacg
    26281 ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc acgttcggct
    26341 tcacgcactt tctgcacgat aacctgtttt gccgtctggg tagtgatacg gtcaaaggta
    26401 acagactcaa tctgatcttc aacgtaatcg cccaggttca ggctttcatc ttcataacgt
    26461 gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac taaccagcga
    26521 cggaaggtgt caaaatcacc gcttttgcga tcgatctgta cgcggacgtc gatctcttgt
    26581 tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg
    26641 cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc tttgttcatc
    26701 gcgggctttt cacctcatcc agactattaa aagtggggaa ccaggttcgc cttctggata
    26761 ttactcagcg cgaacacttc atctttacct tcgacggtaa ctgtgatcat ttcaccgtct
    26821 accgctttga taacgccctg ccatttacga cggttttgta ccgccatacg gagaaccaga
    26881 gtcacctctt ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga
    26941 cccggtgagg agacttccag gttataagca acggtgatgg gatcttcaac atccagcaca
    27001 gcacttacct ggtggctcac atcagcacaa tcatcaacat tgatgccatc ttcactatca
    27061 atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa
    27121 cccagggcct caactggcgc agtaatcatc tctgttaatt tttgctctaa tgtggacaag
    27181 cccaccccca agacataaaa aaagggccta aagcccagtt attctgtagt cagataacaa
    27241 aaaaccccga taaatcgggg ctttatataa ctgaacccta taaccgcaac tgcggtctgg
    27301 agcactttcc agaaggattt tttcaaatcc cactacgaag gccgaagtct tcacagtata
    27361 tttgaaaaag gactctaagg gaaa
//
LOCUS       DAJTVJ010000051        26734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000051 DAJTVJ010000000
VERSION     DAJTVJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.051"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            112..384
                     /locus_tag="OO448_002074"
     CDS             112..384
                     /locus_tag="OO448_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6337280.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            477..560
                     /locus_tag="OO448_002075"
     CDS             477..560
                     /locus_tag="OO448_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337281.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            660..1481
                     /locus_tag="OO448_002076"
     CDS             660..1481
                     /locus_tag="OO448_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6337282.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1520..1849)
                     /gene="mdtI"
                     /locus_tag="OO448_002077"
     CDS             complement(1520..1849)
                     /gene="mdtI"
                     /locus_tag="OO448_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6337283.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1836..2201)
                     /gene="mdtJ"
                     /locus_tag="OO448_002078"
     CDS             complement(1836..2201)
                     /gene="mdtJ"
                     /locus_tag="OO448_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6337284.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2613..3647
                     /gene="tqsA"
                     /locus_tag="OO448_002079"
     CDS             2613..3647
                     /gene="tqsA"
                     /locus_tag="OO448_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6337285.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3672..5060)
                     /gene="pntB"
                     /locus_tag="OO448_002080"
     CDS             complement(3672..5060)
                     /gene="pntB"
                     /locus_tag="OO448_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6337286.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5071..6603)
                     /gene="pntA"
                     /locus_tag="OO448_002081"
     CDS             complement(5071..6603)
                     /gene="pntA"
                     /locus_tag="OO448_002081"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6337287.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7127..8071
                     /locus_tag="OO448_002082"
     CDS             7127..8071
                     /locus_tag="OO448_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6337288.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8257..9639
                     /locus_tag="OO448_002083"
     CDS             8257..9639
                     /locus_tag="OO448_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6337289.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9676..10398
                     /gene="folM"
                     /locus_tag="OO448_002084"
     CDS             9676..10398
                     /gene="folM"
                     /locus_tag="OO448_002084"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6337290.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10395..10730)
                     /locus_tag="OO448_002085"
     CDS             complement(10395..10730)
                     /locus_tag="OO448_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6337291.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10859..11578
                     /gene="rstA"
                     /locus_tag="OO448_002086"
     CDS             10859..11578
                     /gene="rstA"
                     /locus_tag="OO448_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6337292.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11582..12877
                     /gene="rstB"
                     /locus_tag="OO448_002087"
     CDS             11582..12877
                     /gene="rstB"
                     /locus_tag="OO448_002087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6337293.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLAIV
                     HSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12953..13882
                     /gene="tus"
                     /locus_tag="OO448_002088"
     CDS             12953..13882
                     /gene="tus"
                     /locus_tag="OO448_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6337294.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13879..15282)
                     /gene="fumC"
                     /locus_tag="OO448_002089"
     CDS             complement(13879..15282)
                     /gene="fumC"
                     /locus_tag="OO448_002089"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6337295.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15425..17071)
                     /gene="fumB"
                     /locus_tag="OO448_002090"
     CDS             complement(15425..17071)
                     /gene="fumB"
                     /locus_tag="OO448_002090"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6337296.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17270..18445
                     /gene="manA"
                     /locus_tag="OO448_002091"
     CDS             17270..18445
                     /gene="manA"
                     /locus_tag="OO448_002091"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6337297.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18546..20054
                     /locus_tag="OO448_002092"
     CDS             18546..20054
                     /locus_tag="OO448_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6337298.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20235..21545)
                     /gene="uidC"
                     /locus_tag="OO448_002093"
     CDS             complement(20235..21545)
                     /gene="uidC"
                     /locus_tag="OO448_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6337299.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21584..22957)
                     /gene="uidB"
                     /locus_tag="OO448_002094"
     CDS             complement(21584..22957)
                     /gene="uidB"
                     /locus_tag="OO448_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6337300.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22954..24765)
                     /gene="uidA"
                     /locus_tag="OO448_002095"
     CDS             complement(22954..24765)
                     /gene="uidA"
                     /locus_tag="OO448_002095"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6337301.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25154..25744)
                     /gene="uidR"
                     /locus_tag="OO448_002096"
     CDS             complement(25154..25744)
                     /gene="uidR"
                     /locus_tag="OO448_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6337302.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25965..26711)
                     /gene="hdhA"
                     /locus_tag="OO448_002097"
     CDS             complement(25965..26711)
                     /gene="hdhA"
                     /locus_tag="OO448_002097"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6337303.1"
                     /translation="MRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHV
                     VDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMA
                     DFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAAS
                     HLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIA
                     NAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6711 a         6928 c         6418 g         6677 t
ORIGIN      
        1 tgccattccc agggatatag ttatttcaac ggccccgcgg tggggttaaa tgaaaaaaca
       61 aattgagggt atgacaatga aaaagtatta gctctggttg ttgccgctgc tatgggtctg
      121 tcttctgccg cctttgctgc agagactgcg accacacctg ctccgactgc gacgaccacc
      181 aaagcagcgc cggcgaaaac gacacatcat aaaaaacagc ataaagcagc acctgcccag
      241 aaagcgcagg cggctaaaaa gcatcataaa aatacgaaag ctgaacagaa agcccctgaa
      301 caaaaagcgc aggcagcgaa gaaacacgcc gggaaacaca gccatcagca accggcaaaa
      361 cctgctgcac aacccgcagc gtaagttttc aacagtaatg ctggcgcgcc ccctcgcgcc
      421 tgaaaattac ggtgctaagc gggtaacgtt tagcaccgcc tttagcggag ggtagtatgg
      481 tgggccgtta tcgctttgag ttcattctta tcatccttat tttatgcgca ctgattgccg
      541 cccgttttta tctttcctga ctgtagttat ctgattttac tcccactttc atcccgtccc
      601 gtctatagta tttatgaggg tttgctttta ataatcataa ttacccacca gagtgtgata
      661 tgcgtacaac cattgctgta gtgttgggtg caattagttt gacgtctgct tttgtgtttg
      721 cagataaacc agacgttgcc aaatcggcaa acgatgaggt cagcaccctg ttttttggtc
      781 atgatgatcg tgtgccagtg aatgacacga cccaatcacc gtgggatgcg gttgggcaac
      841 tggaaacggc cagcggcaat ttatgtacgg cgacgctgat tgcacccaat ctggcattaa
      901 cggcaggaca ctgtttattg acacctccaa agggtaaagc cgataaagca gtggcgctgc
      961 gttttgtgtc aaataaaggt ctttggcgct atgagatcca cgacatagaa ggccgcgttg
     1021 atccgacact gggaaagcgg ttaaaagcag atggggatgg ttggattgta cctcccgcag
     1081 ccgcgccgtg ggacttcgga ttgattgtgc tacgtaatcc cccttctggc attacaccgt
     1141 tgccgttatt tgagggagat aaagccgcgc ttactgccgc attaaaatcg gctggccgta
     1201 aagtaactca ggcgggatac cctgaagatc atctcgatac gttgtacagt catcaaaact
     1261 gtgaagtgac tggctgggcg caaacgtcgg tgatgtcaca tcagtgcgat accttgccgg
     1321 gtgacagcgg ttcgcctctg atgttgcata ccaatgacgg ctggcaatta attggggtgc
     1381 aaagttcggc tcctgccgcg aaagatcgct ggcgcgccga taaccgggcc atttctgtta
     1441 ccggttttcg cgacaagctg gatcaactgt cgcaaaaata atgttcaaac gctgcccgac
     1501 tgtgcgggca gcgtgttcat caggcaagtt tcaccatgat cattccagcc aacagcaaga
     1561 ccaggccaat ccagccttta cgatttaacc gttgaccaaa caagatccaa cctgcggcta
     1621 acgtggcggc aataccaaac ccgccccaca atgcataagc gacagacaag tcgatccctt
     1681 taacggcctg agaaagcgca ctaaaggcag ccagcaccgc cgccagggag agcaagccaa
     1741 atattttgcg acgaaagccg tcagaaaatt tcaaaaagac gttagcaacg atttccagca
     1801 cgattgccaa tgccagccag gcggcgtgaa cccattcaaa ctgcgccatg gttcacctcc
     1861 agttcaggtt tacgcgcttt acgggtacct gattttatca acacaatccc ggcgaccagg
     1921 gtggttaacc cggcaatttt catcagcgat aaactttcgt cgaataacaa aacgctaaac
     1981 aaggtaataa ataaaatacc gataccttcc cacagcgcat aagctacgcc taaggcgatc
     2041 tttttaacgg cgaaagagag aaatatatac gacagagaaa tcatcaccag cattaaaata
     2101 aagccgccat ttccctcact gacgctcgcc catttcattg acagcgtacc ggtaatttct
     2161 gtagcaatag ccaggcctaa taaaatccaa taaatataca ttgtccttct cctgcaagag
     2221 aattatttta attttcgctt aattcagcga aaccaaagta aagtggtcaa gccccgcgca
     2281 tagggcaagc ttaggcagaa gaaaggacta aagcgcgttg cgccagtgct gctcacctac
     2341 gagcaagata gtggatgagg tacgaagatg ggtaaatgtt gaaaataacg tcctgaacaa
     2401 attgtccata atattacaat tatccgcagt gttgcttctc gtcatcgcgg atgataattg
     2461 tcctcggtag ttgaacacgc ctgatttgta tcatagctta agaattaact caaaatattt
     2521 tcacttcttt acctgagcgg tttgattttc gttatgatga cggagcgaaa aagacattat
     2581 tattagcaaa ggaagaaaaa acggggacaa gcatggcaaa gccgatcatc acgctcaatg
     2641 gcctaaaaat cgtcattatg ttgggaatgc tggtcattat tctctgcggt atccgttttg
     2701 ccgccgagat catcgtgccg tttattctcg cattatttat tgctgttatt cttaacccgc
     2761 tggtgcaaca catggtccgc tggcgagtgc cgcgtgtact ggcggtgtcg attttgatga
     2821 ccatcatcgt gatggcgatg gtgttgctat tagcttatct gggttccgcg ctcaacgagt
     2881 tgacgcggac gttaccgcaa tatcgcaact ctattatgac gccgctgcaa gctcttgagc
     2941 cgttgttgca acgcgtaggg attgacgtct cagttgacca gctggcgcat tacattgatc
     3001 cgaacgcggc gatgacgttg ctcaccaact tattgacgca gttatctaat gccatgtcat
     3061 caatattttt attgctgctg acggtgctgt ttatgctgct cgaagtgcca caattgcccg
     3121 gaaaatttca gcaaatgatg gcgcgtccgg ttgaagggat ggcggcgatt caacgtgcaa
     3181 ttgacagtgt ttctcattat ctggtgctga aaacagccat cagcatcatc accggcctgg
     3241 tcgcctgggc gatgctcgcc gcactcgatg ttcgcttcgc ttttgtctgg ggattgctgg
     3301 cctttgcgct taattacatc cctaatattg gttcagttct cgcggcaatc ccccctatcg
     3361 ctcaggtact ggtgtttaat ggcttctacg aagcgttgct ggtgctggcg ggatatctgc
     3421 tgattaatct ggtcttcggc aatattctgg agccgcgcat catgggacgt gggctggggc
     3481 tttccacatt ggtggtattt ttgtcgttga ttttttgggg atggttgtta ggaccggtgg
     3541 gtatgctgat ttccgtgccg ttaacaatta ttgtcaaaat tgcgcttgaa caaacagcgg
     3601 gaggtcaaag catcgccgtt ctgttaagcg atctcaataa agagtgacgg cctccgcaga
     3661 ggccgtcagg gttacagagc tttcaggatt gcatccacgc tagctttggc gtcaccaaac
     3721 agcatgtggg tgttttcctt gaagaacagc gggttttgca caccagcata gccagtgttc
     3781 atcgaacgtt taaagacaat cacgttctgc gctttccaca cttccagcac aggcatacca
     3841 gcaataggac tcttcggatc atcctgcgcc gccgggttaa ccgtatcgtt agcaccaatc
     3901 accagtacgg tatcggtatc agcaaagtca tcgttgatct cgtccatttc cagcacgatg
     3961 tcatacggta cttttgcttc agccagcaat acgttcatat gtccaggcaa acgccccgca
     4021 accgggtgga taccgaaacg cacgttgata ccacgagcgc gcagtttctc ggtaatttca
     4081 gcgacaggat attgcgcctg cgcgactgcc atgccgtacc ccggagtaat gatcactgaa
     4141 tgggagtttt tcagcagttc cgctgtctct tctgcggtga tttcgcggtg ctcgcccact
     4201 tcctgatcat cgccagtaga agagccgtcg gtgccgaaac cacccgcaat aacgctgata
     4261 aaggaacggt tcatcgcctt acacataatg taagaaagga tagcccccga agaaccgacc
     4321 agcgcaccgg tcacaatcag caggtcgttg ctgagcataa agcccgcagc cgcagccgcc
     4381 cagccggagt acgagttcag catcgacacc accactggca tatctgcacc accgatggag
     4441 gcgactaaat gccagccgaa taccagcgca atggcggtca ttattagcaa tgccagcact
     4501 tgcaggccga cgctgtccgt gcgaacaaat acaatcagca gcaggaagga aacgaccaga
     4561 gccgccaggt tcattttgtg acggtttggc agcatcaacg gtttagacga aatcttgcca
     4621 cacagtttgc cgaacgccac caccgaaccc gtgaacgtta ccgccccgat gaagataccg
     4681 aggaacactt ccgtcaggtg aatattgacc agaatcggtg ccattcccgc gtcatgatgc
     4741 agatagctgt taaagccaac cagcactgcc gccagaccca cgaagctgtg caggatcgcc
     4801 accagttctg gcatttcggt catttcaact ttcttcgcca gacggatacc aattgcccca
     4861 ccaatgacca tcgccagcaa gatccagcca acattacccg tatccggtcc aaaaatggtt
     4921 gcgattaacg caatcgccat cccggcgata ccgaagttgt taccctggcg agacgtttca
     4981 tgtttcgaaa gaccggccag actgaagata aacaggatcg cggcaacaat gtatgcagct
     5041 gtaactaatc ctccagacat atgttacccc ttaatttttg cggaacattt tcagcatgcg
     5101 ctgagtcacg gtgaagccac cgaaaatatt aatgctggct ataagcaccg cgataaaact
     5161 aaggaagcta acccagccgc cctggccaat ctgcaacagt gctccgacaa caataatccc
     5221 tgaaatcgcg ttggtgaccg acatcaacgg tgtatgcagc gcgtgcgata cattccacac
     5281 cacgtaataa ccgacaacgc aggccagcgc gaaaacggtg aagtgcccaa gaaactcttt
     5341 cggcgcaacg cttgccagcc aaccgaaaag gatgattgcc agcgccatca acgcgtattt
     5401 acgccacggt gagcaggcac atttttcctc agttttcact tccggtgccg ctttttgtgc
     5461 cgcctgcggc tgagctgata cctgaatcgg cggtgccggc caggtaattt cgcccgcacg
     5521 gatcacggtc acgccgcgaa tcaccacatc atcaaaatca acagtgatat tgccgtcttt
     5581 ctctttgcac aacagtttca gcagattaac gaggttagta ccgtaaagct gcgaggattg
     5641 cgtcggcaga cggcccggaa gatcggtata accaatcact ttgacaccat tttccgtagt
     5701 gaagatttca cccggcacgg tgtattcaca gttgccaccg ttttgggctg ccaggtcgac
     5761 aatcacactg cccgccttca tggagtcaac catttcacgg gtaattagct tcggcgctgg
     5821 tttgcctgga ataagcgcgg tggtgacaat gatatcgacc tcttttgcct gggcggcaaa
     5881 gagttccatt tccgctttaa tgaacgcgtc cgacatcact ttggcatagc catcgccact
     5941 gcccgcttcc tctttaaaat ccagctcgag gaattccgcg cccatacttt gaacttgttc
     6001 tttcacttcc ggacgggtgt cgaatgcacg cacaatcgcg ccgagactgt ttgctgcgcc
     6061 aatggcggcc agacctgcaa cacccgcacc aatcaccatc acttttgccg gtggcacttt
     6121 cccggccgca gtaatttgcc cggtaaagaa gcgcccaaat tcatgtgccg cttcaacaat
     6181 ggcgcgataa ccggcgatgt tcgccatcga gcttagtgcg tccagcgatt gtgcgcgtga
     6241 gatacgcggc acagaatcca tcgccatcac ggtcacgtta cgttccgcaa gtttttgcat
     6301 taattccgga ttctgcgcag gccagataaa actcaccagc gttgtcccag gattcagtaa
     6361 cgcaatttca tcatctaacg gcgcattgac cttcagaatg atctctgact gccagacgct
     6421 attcccttct acaatttcag cgcccgcttg cacaaacgct ttatcgtcaa aacttgccag
     6481 ttgacccgcg ccgctctcta ccgcgacggt aaaacccagt ttcagcagct gttccactgt
     6541 ttttggcgtt gctgcaacac gggtttcatt ggttaaccgt tctcttggta tgccaattcg
     6601 catgatattc ccttccatcg gttttattga tgatggtttg cctgtgtcag gagccacaca
     6661 agccgctcat gtacgagcta aatgttactc cgttaaaata aattagtaac aaacgcccta
     6721 taacgtactg aaaattatgc ctgtgatcta gcgccaaaaa tcagtatttc ggcgtgaact
     6781 cgcaaaatat taacgattca gccgtgatag tgggataaac accttagaac gccggataaa
     6841 gactgataat tgtcttcgac ggccgggtaa aacgagacat cgccccggca cgattcacta
     6901 cttaacatta aattaactta tacaattcag ttgcttcagt agtaatgatg ctgatacggc
     6961 tgttttttaa gcatagacgg tcatttgagc aggattaaaa ttggcttaag gaatgtgata
     7021 tgaaaaatga cgcagacagt tacaccgttt aaatgcaata atcagccacg tttctcgtta
     7081 ataacaatac cagtacctgg tttgcgcaag gcgaaggatt atttttatga agcttaagaa
     7141 caccctcctg gcgtcggcac tgctttctgc tacggcattc tccgttaacg cagcaacaga
     7201 actgacaccg gagcaagcgg cagcggttaa accttttgac cgtgtagtgg ttaccggtcg
     7261 ttttaatgct attggcgaag cggtgaaagc cgtttctcgt cgcgcagata aagaaggtgc
     7321 cgcctctttt tatgttgtcg acacttctga ttttggtaac agcggtaact ggcgtgtggt
     7381 cgctgacctc tataaagccg atgctgaaaa agcagaagaa acaagtaatc gcgtaattaa
     7441 cggtgttgtc gaactgccga aagatcaggc tgttctgatt gaaccgtttg acacggtcac
     7501 cgtccagggc ttctatcgta gccagccaga agtcaatgat gccatcacca aagcggcaaa
     7561 agcgaaaggt gcctactctt tctacatcgt tcgtcaaatc gatgccaacc aaggcggcaa
     7621 ccagcgtatt actgcattca tctataaaaa agatgctaag aaacgtatcg tccagagccc
     7681 ggatgtgatc ccggcagatt ccgaagcagg acgtgcagct ctggctgccg gtggcgaagc
     7741 cgcgaagaaa gttgagatcc taggtgttgc gactaccgca tcaccaagtt ctgaagtcgg
     7801 tcgtttcttt gaaacccagt catcaaaagg cgggcgttac accgtcatgc tcccggatgg
     7861 cactaaagtc gaagaactga acaaagcgac cgcagcgatg atggtcccgt tcgacagcat
     7921 caaattctct ggcaactacg gcaacatgac cgaagtctcc tatcaggttg cgaaacgtgc
     7981 cgcgaagaaa ggtgctaagt attaccacat cacccgccag tggcaggaac gtggtaataa
     8041 cctgaccgtc agcgcagatc tgtataaata actattcagt tactcctata aggcggcttg
     8101 atgccgcctt tcgcattttt agtcacaaat ttccccgcga ccattgcatg cagacacatc
     8161 tcttcgtaaa atcccgcgct tcagtggcaa actccatttt tatgcgtttt gccctaatat
     8221 ttattcttct tatcacgttt taatcactgg atatcgatga aaaagaaact gggactgagc
     8281 gcactcaccg cgctggtatt aagctcaatg ctgggcgcgg gtgttttcag tctgccgcaa
     8341 aatatggcgg cagttgccag cccggcagca ctgctcatcg gctggggtat tactggcgct
     8401 ggcattttat tgctggcctt tgccatgctg atcctcacgc gcattcgtcc cgaacttgac
     8461 ggcggtatct ttacctatgc ccgcgaaggg tttggcgagc taatcggctt ttgttccgca
     8521 tggggatact ggctgtgcgc agtcatcgcc aacgtctcct atctggtgat cgttttttcc
     8581 gcgttaagct tttttacgga cacgccggaa ttgcgcctgt tcggcgatgg taatacctgg
     8641 caatcgatcg tcggtgcatc ggctttattg tggattgttc actttttgat cctgcgcggg
     8701 gtgcaaaccg ctgccagcat taacctggtg gcgacactgg caaaattgtt gccgctgggt
     8761 ctgtttgttg tgctggcgat gatgatgttc aaactggata ccttcaagct cgacttcacc
     8821 ggacttgcac ttggcgtacc cgtttgggaa caggtgaaaa acaccatgct gatcaccctg
     8881 tgggttttca ttggtgtgga aggtgccgtg gttgtttctg cgcgtgcgcg taataaacgt
     8941 gatgttggca aagcgacact gctggcggtt ctctccgctc tgggcgttta cttgctggta
     9001 acgctgcttt cgctgggtgt ggtggcacgt cctgaactgg ctgaaattcg taacccgtca
     9061 atggccggtc tgatggtgga aatgatggga ccatggggcg aaatcatcat cgctgccggt
     9121 ttgatcgttt ccgtttgcgg cgcgtactta agctggacca tcatggcggc agaagttccg
     9181 ttcctggctg ccactcataa agcattccca cgcatttttg cgcgccagaa cgcacaagct
     9241 gcgccatccg cctcactgtg gttgaccaac atctgcgtgc aaatttgtct ggtactcatc
     9301 tggctgaccg gttccgatta caacacgttg ctgaccatcg cctcagaaat gattctggtg
     9361 ccctatttcc tggtcggcgc gttcttgctg aaaatcgcaa cccgtccact gcataaagcg
     9421 gtaggtgtcg gtgcctgcat ttatggctta tggttattgt acgcctctgg tccaatgcac
     9481 ttgctgttat ccgttgttct gtatgcgccg gggctgctgg ttttcctgta tgcgcgcaaa
     9541 acgcatacac atgataatgt actgaaccgt caggaaatgg tgctgattgg catgctgtta
     9601 attgcctcag ttccggcgac ctggatgctg gtgggataac gttcatccca tcgttggata
     9661 gcaaaggaga taacgatggg taaagcccag cccttgccaa tattaattac tggcggaggt
     9721 cgtcgcatcg gcctcgccct cgcctggcat ttcattaatc aaaagcaacc ggtgattgtc
     9781 agctatcgga cgcactatcc agccattgat ggactgatta aggcaggtgc gcagtgtatt
     9841 caggctgatt tttcgaccaa cgacggtgtg atggcgtttg ccgatgaagt actaaaaagc
     9901 ccccatggtc tgcgtgctat tttgcataac gccagtgcgt ggatggcgga aaaaccgggt
     9961 gcgccactgg ccgacgtact ggcttgcatg atgcagatcc acgttaatac cccatacctg
    10021 ctcaaccatg cgctggaaag attactgcgt gggcacggac acgccgccag cgatatcatt
    10081 cactttaccg attatgtggt ggagcgcggt agcgacaagc atattgcgta tgctgcaagc
    10141 aaagcggcac tggataatat gacccgctcg tttgcccgca agctggcacc ggaagtgaaa
    10201 gtgaactcta ttgctccatc gctgatcctg tttaatgaac atgatgatgc cgaatatcga
    10261 caacaggcgc tgaataaatc actgatgaaa accgcgcctg gcgagaaaga agtgatcgac
    10321 ctggtcgatt acttacttac cagttgcttt gtcaccggac gcagtttccc acttgatggc
    10381 ggtcgtcatc tgcgttaatg cagctttatc cagcatataa tcaacaccca tgcgcttatc
    10441 ccccaacacg aaacagaacc aacaaaagcg gcgggcaagc gcatcattcc ggtgaaatac
    10501 cacagcgaca ccaggtaaac aaaataggga ataatcgacc acatactaaa aatgatggtt
    10561 gcgcgtaagg cttcaatgcc gcgttcgctg gcaacaatat aatgcgcgat aagcgcaaag
    10621 gtcgggaaaa gtggaatcag cccggcgata taataatttt tcgtttttgc taaaacacca
    10681 atcaacagca ctaccagcgc accgagcgcg gctttgatta ccagccccat ttttttacct
    10741 taacacttcc ataacaagtc atcagtagaa tacctgatga aaacttgttt agaaacgatt
    10801 gatagtaagt aaaaacagcg cggtgtattg tgacgttttt atatctaccg tgaatgttat
    10861 gaacactatc gtatttgtgg aagatgatgc ggaagtcggt tcactgattg ccgcgtacct
    10921 ggcaaaacat gatatgcagg ttaccgtaga gccgcgcggc gaccaggccg aagaaaccat
    10981 tttgcgagaa aatccggatt tggtgttact cgacatcatg ctaccaggca aggacggcat
    11041 gaccatttgt cgtgatttac gcgcaaagtg gtctggaccg attgttcttc taacctctct
    11101 cgatagcgat atgaaccaca tcctggcact ggaaatgggt gcctgcgact atattctcaa
    11161 aacgacgccc cctgctgttt tgctagcgcg tttacgtttg catttgcgtc agaatgagca
    11221 agccacactg accaaaggtc ttcaggaaac gtctctgact ccctacaaag ccctgcattt
    11281 cggcacgttg accatcgatc ccatcaaccg cgtagtcacc ctggctaaca ctgaaatctc
    11341 gctctcgaca gctgatttcg aattattgtg ggaattagct acccatgccg ggcaaatcat
    11401 ggaccgcgat gcattgctga aaaatttacg cggcgtcagt tatgacggac tggatcgtag
    11461 cgtggacgtg gctatttcgc ggttaagaaa aaaactgctc gataacgccg cagaacctta
    11521 tcgcattaaa actgtgcgta acaaaggcta tctttttgcg cctcatgcat gggaataagc
    11581 gatgaaaaaa ctgtttatcc agttttacct gttattgttt gtctgcttcc ttgtgatgtc
    11641 tctgctggtt gggctggtgt acaaatttac cgccgaacgc gcgggcaaac agtcgctgga
    11701 tgatttgatg aacagttcac tgtatctgat gcgcagcgaa ttgcgtgaga tccccccaca
    11761 cgactggggt aagacgctga aagagatgga tttaaatctc tctttcgatc tgcgtgtcga
    11821 gccactgagt aaataccatc ttgatgatat ttccatgcac cgactgcgtg gcggcgaaat
    11881 tgtcgccctg gacgatcagt acacgttttt gcagcgtatc ccgcgcagcc actacgtgct
    11941 ggcagttggt cctgttcctt atctttatta tctccatcag atgcgattgc tggatatcgc
    12001 cctgatcact tttattgcta tttccctcgc ctttccggtg tttatctgga tgcgtccgca
    12061 ctggcaggat atgttaaaac tggaagcagc ggcgcaacga tttggcgatg ggcatctcaa
    12121 tgaacgtatc cactttgatg agggttcgag ctttgaacga cttggtatcg catttaacca
    12181 gatggcggac aatatcaacg ccttgattgc cagcaaaaaa cagcttattg acggtatcgc
    12241 tcacgaactg cgaacaccgt tagtgcgcct gcgttatcga ctggagatga gcgataacct
    12301 gagcgccgcc gaatcccagg cgttgaatcg tgatatcagt caacttgaag ctttaattga
    12361 agagctgctg acttatgccc gactcgatcg cccacaaaac gagcttcatc ttagcgaacc
    12421 agacctgccg ttgtggctgt caacgcatct ggcagacatt caggcagtaa cgcccgataa
    12481 aacggtacgg attaaaacgc tcgcgcaagg ccattatgcg gcgttggata tgcgcttaat
    12541 ggagcgcgtg ctggataatt tgctcaataa cgccctgcgg tactgccatt caacggttga
    12601 aaccagcctg ctactgtcgg ggaatagagc gacattaatt gttgaggatg atggcccagg
    12661 gattgcccca gaaaaccgcg aacatatctt tgaacctttt gttcgcctcg atcccagccg
    12721 ggatcgctca accggcggct gcgggctggc aattgtccac tctatagcac tggcaatggg
    12781 cggtacggtt aattgtgaca ccagcgaact gggtggtgct cgcttctcgt ttagctggcc
    12841 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
    12901 caccaccact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
    12961 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctgc
    13021 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
    13081 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
    13141 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
    13201 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
    13261 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
    13321 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
    13381 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
    13441 cgaccccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
    13501 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg
    13561 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
    13621 acagaacgcg aagttaaaaa tcaaacgtcc ggtgaaggtg cagccgattg cccgcgtctg
    13681 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccgctga ttgcactgat
    13741 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
    13801 caatgtgcag caccgttata aaccacaggc gcagccgctt cgtttgatca ttccacggct
    13861 gcacctgtat gttgcggatt aacgcccggc tttcatactg ccgaccatct gttctggccg
    13921 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
    13981 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
    14041 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
    14101 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
    14161 gcgcaccgat tgcaggaaat tgtggatcac cattggacgg aagacgttca gttcaaagtt
    14221 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
    14281 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
    14341 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
    14401 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
    14461 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
    14521 ggtaatgact gccagttcat ctgctacgcg acgcgcatac tccggatggg tatttagtcc
    14581 agtacccacc gctgtaccgc cgagagccag ttccgctacg tgaggcaggc tgtattcgat
    14641 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ccagtgttag
    14701 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
    14761 tttttcactc agtgtctgtg tcagggtttt aacctgcgga atgagttgct tgcgcagcgc
    14821 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
    14881 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acgccgccga gtaattcact
    14941 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
    15001 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
    15061 ccgaatggcg ctcgctttct cttcagacaa caagcctaaa tcttcattaa cttttgccgc
    15121 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
    15181 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
    15241 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
    15301 ttaatttttt ctttctgttt tgccttcgtt aagcaacttt tagctcactt attatttact
    15361 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
    15421 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
    15481 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
    15541 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
    15601 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
    15661 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
    15721 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
    15781 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtactgcg
    15841 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccga
    15901 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
    15961 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
    16021 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
    16081 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
    16141 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcgtat gtcgtgagcg aagtatttac
    16201 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagcaattct ttttccagtt
    16261 ccacatcgcg gaatgcctga ccgtgctcat tcccttccgt tggcagttcg tcatagtatt
    16321 tagcggaagc cagtttcact gttttaaggt tagtttctgc agaagttcca ccaattacga
    16381 acgcaatatg atacggagga caggccgccg tacccagcgt gcgcatcttc tcaaccaggt
    16441 aatttttcag tttccccggc gttagtaacg ctttggtttc ctgatagaga tacgtcttgt
    16501 ttgccgaacc accacctttg gcgatacaga ggaatttgta ctcgtcgcca tcaacggcat
    16561 aaagatcgat ctgcgctggc agattggtgc cggtattcac ctctttatac atatccagcg
    16621 gcgcgttttg cgagtagcgc aaattatctt cgatataagt gttatagaca ccgcgcgcca
    16681 gcgccgcttc atcacagcca ccggtccata cacgctgccc ttttttacca acaataatcg
    16741 cagtgccggt atcctgacag gttggcagaa cacctttcgc cgcgatgtcg gagttgcgca
    16801 ggaattgcag cgccacatat ttatcatttt cgctggcttc cgggtcacgc agaatgtcgg
    16861 ccacctgttg ttggtgagcc ggacgcagca taaatgacgc atcatgaaac gcctgacgcg
    16921 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
    16981 cgctaacgtg ttcgctggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
    17041 gagcctgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
    17101 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacactt
    17161 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
    17221 tcttgcttta atagtgggat taatttccac attaaaacag ggattgatca tgcaaaaact
    17281 cattaactca gtgcaaaact atgcctgggg cagcaaaacg gcgttgactg aactttatgg
    17341 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
    17401 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
    17461 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
    17521 gttcaaagta ttatgcgcag cacagccgct ctccattcag gttcatccaa acaaacgcaa
    17581 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
    17641 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
    17701 gatgaacgcg tttcgtgagt tttccgagat tgtctcccta ctccagccag tcgcaggtgc
    17761 acatccggca attgctcact ttttacaaca gcctaatgcc gaacgtttaa gcgaactgtt
    17821 cgccagcctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
    17881 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
    17941 cccggaagac agtggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
    18001 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
    18061 agtgatggcg aactccgata acgtgctgcg tgcgggtctg acgcctaagt acattgatat
    18121 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
    18181 gccggtgaaa caaggtgcag aactggactt cccgattcca gtggatgatt ttgccttctc
    18241 gctgcatgac cttagtgata aagaaaccac cattagccag cagagtgccg ccattttgtt
    18301 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
    18361 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt
    18421 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
    18481 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
    18541 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
    18601 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
    18661 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
    18721 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga
    18781 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
    18841 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
    18901 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
    18961 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
    19021 tcaagccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
    19081 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
    19141 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
    19201 ccgacggttc cagcacgctg gcaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
    19261 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
    19321 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
    19381 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
    19441 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
    19501 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
    19561 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
    19621 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
    19681 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
    19741 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag tttatgactc
    19801 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
    19861 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
    19921 tcaccaccag cctgcaatat actaacggtc agataacgtt aaacgggcag aaaatgccac
    19981 tggaagattt cgttggtatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
    20041 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcacttcggc gttttcatct
    20101 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaac
    20161 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacaccgcat ccggcataaa
    20221 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
    20281 gacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
    20341 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
    20401 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
    20461 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatctgtgc aaatgtcagt
    20521 gtaaattgcg gcgttaaacg atagttgacg cccaattgcc aggactgcat tccttcacgg
    20581 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
    20641 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
    20701 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
    20761 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
    20821 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
    20881 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
    20941 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
    21001 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
    21061 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
    21121 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctgattta
    21181 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
    21241 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
    21301 tatgccccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
    21361 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atccgccctg
    21421 cgcaattcgt ttttcagtct gatgcgtagt cccgcctcat cgtcagccag ttgcgccgca
    21481 taagcagaga taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    21541 ctcatagttg cacctttgat gttctgatta tttttattga ttattaatta gtgatatcgc
    21601 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    21661 ttttatccgt aagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    21721 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttgc acctgattgg
    21781 caatatatcc gcttaacccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    21841 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    21901 attcaccgta ttctacggta tcagcttcca gtgcccacat cactgtcata gtaacgccct
    21961 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    22021 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    22081 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    22141 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    22201 acaacgacga ggcgctgacc gcaaaggtcg aaatcagcac acacagcgca ccgatgcaca
    22261 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    22321 caacgatacg taccacattc tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    22381 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    22441 agttcttaat gctcggccct atcagaaatg ccaggcagac aaaggtcaat gaagcggcaa
    22501 tcccacgagc cgcgcccaga cgggcgcggg attgtggttg ttgggtcatc gcggtagcaa
    22561 gtgaaccata aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat
    22621 atgcatacac tactttgcgg ctatggctcc agtcggtcgg tacccagaat accagcacgc
    22681 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    22741 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatcg aatacccgca
    22801 ccagtaacag catggtgccc gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    22861 tcaacaggaa gagcaccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    22921 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    22981 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    23041 gcggtcgcgg gtgaagatcc ccttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    23101 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    23161 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    23221 tgagtgcagc ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    23281 tttctcctgc caggccagaa gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    23341 gctttggaca taccatccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    23401 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    23461 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    23521 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    23581 cagctcttta atcgcctgta agtgcgcttg ctgagtttcc ccgttgactg cctcttcgct
    23641 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    23701 gacagcagca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    23761 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    23821 gtggtcgtgc accatcagca cgttatcgaa tcctttgcca cgcaagtccg catcttcatg
    23881 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    23941 tgccactgac cggatgccga cgcgaagcgg gtagatatca cactctgtct ggcttttggc
    24001 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    24061 ttgcaaagtc ccgctagtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    24121 ttcaacgctg acatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    24181 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    24241 cattacgctg cgatggatcc cggcatagtt aaagaaatca tggaagtaag actgcttttt
    24301 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    24361 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    24421 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    24481 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    24541 tataaagact tcgcgctgat accagacgtt gcccgcataa ttacgaatat ctgcatcggc
    24601 gaactgatcg ttaaaactgc ctggcacagc aattgcccgg ctttcttgta acgcgctttc
    24661 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    24721 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    24781 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    24841 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    24901 aaaaaccaaa aactgcaata cccacagcgt gatattgatc gcattaatct ttaaaacggc
    24961 gttgacagaa gccataaaat aagcaaccga acgttcgttc gagcatattg ggaacgataa
    25021 actctcatga catatggcta cggtgaatat ttttgggggg gaattctccg atcccggcta
    25081 gattgtattt tgtacaggga aattaaagta tgaaacgcgg gcacagttat caccagcccg
    25141 cgaaatgaga ggatcaggat gcggttaaga taccgccaat catcgcgcgt aatccctgag
    25201 caaacgacgc ttcacgcgcc agatcttccg cttcgatatc ggccagtaca ccgtaggtca
    25261 gatccagcag taaacgtgaa gtcattgacg tgttgatgtc tgggcttatc tcgccttttt
    25321 gctgggcatc acgcatccgc tgggcaacaa attccgtgat cgtcatatgc ttatttttaa
    25381 gcatggcggc gacctgtggg ttacgcatcc cttccgccat aatttcgacc accagcgccc
    25441 gttgcccaaa ggcttcatgg gtgagagaga caatagactc gaccatatag tcaacaaaat
    25501 gaatcccttt aatcggttcc cggaaacgag ccagcgccct ctcctggtcc tgtaagataa
    25561 tcgcctgaat caaggcttct ttggagatga aatggtgata gagcgtcccg ggactaatgg
    25621 cgcaagattt acagatggct ttcatcgagg cactgtgaaa tccattttct gaaaaaatct
    25681 ctctggcagc attgaggatc cgggtccgtg tcggttgtgc ttcagtctgc atgttatcca
    25741 tcattaaacc attatagtta gcgtgaataa ctagtttacc gcgttgtcag gtgagcgcct
    25801 atcattgctg ttgaatagtg cgaaggcaca ctctattcgt atacttttca ctctgaaatt
    25861 ggttaaccaa tagccgtgat ttggctgcca gttatttagc agagacgttg atgagtttat
    25921 cccggtaaac aacacgcacg gttgtttacc ggtccgttag tgtattaatt gagctcctgt
    25981 accccaccac cggagacggt gagaatttgt ccgcttaccc agctcgcagc aggcgagcaa
    26041 aggaacagcg ctgcgttagc aatatcttgc ggttggccca gacgtctgat cggcgtgtgc
    26101 tgtaacattt tttgttcaat ttctggtgta ataacggatt tcagggcatc ggttaatatt
    26161 gcccccggcg caatgccatt tacccgaata tttttttcac ccaggtcaaa cgccatattt
    26221 ctgaccagat gactggctgc agctttagat gatgcatagg aagtcatgtt tatattttta
    26281 ttttctgccg ccatagaagt gatggtcaga ataacgccac cgccattttt ttccatttct
    26341 ggcgcaacaa gttgtgacag atggaaaaaa gaaaacacat tcagttcata agcacggcga
    26401 aaatccgcca ttggcatatc aaacggttta ggtccaccgc caccggcgtt gttaaccaga
    26461 atatcaactt tacccagctt actgatagca aagtctgcca gtgcagagag ttcctgttcg
    26521 gaagtaatat cacaacggca ggcaaatgcc tgaccaccca gttgttgaat ttcgtctaca
    26581 acatggttag ctgcgtcggc gttaatatca ctgaccacca cagatgcgcc agctgtcgcg
    26641 aatgtaatgg cgatttcttt accaatacct gcacccgcac ctgtgatgat ggcgcatggc
    26701 gcatggcgca tggcgcatgg cgcattttcc gtcg
//
LOCUS       DAJTVJ010000052        25774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000052 DAJTVJ010000000
VERSION     DAJTVJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.052"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OO448_002098"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OO448_002098"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6337304.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="OO448_002099"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="OO448_002099"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6337305.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="OO448_002100"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="OO448_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6337306.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="OO448_002101"
     CDS             2440..3063
                     /locus_tag="OO448_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6337307.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OO448_002102"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OO448_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6337308.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /locus_tag="OO448_002103"
     CDS             complement(3447..3512)
                     /locus_tag="OO448_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337309.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OO448_002104"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OO448_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6337310.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="OO448_002105"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="OO448_002105"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6337311.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OO448_002106"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OO448_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6337312.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OO448_002107"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OO448_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6337313.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OO448_002108"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OO448_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6337314.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OO448_002109"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OO448_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6337315.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OO448_002110"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OO448_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6337316.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OO448_002111"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OO448_002111"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6337317.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="OO448_002112"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="OO448_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6337318.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="OO448_002113"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="OO448_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6337319.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="OO448_002114"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="OO448_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6337320.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19898..20824
                     /gene="gluQRS"
                     /locus_tag="OO448_002115"
     CDS             19898..20824
                     /gene="gluQRS"
                     /locus_tag="OO448_002115"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6337321.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20863..22281
                     /gene="pcnB"
                     /locus_tag="OO448_002116"
     CDS             20863..22281
                     /gene="pcnB"
                     /locus_tag="OO448_002116"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6337322.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="OO448_002117"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="OO448_002117"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6337323.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="OO448_002118"
     CDS             23127..23711
                     /locus_tag="OO448_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6337324.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="OO448_002119"
     CDS             23816..24556
                     /locus_tag="OO448_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6337325.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25774
                     /gene="htrE"
                     /locus_tag="OO448_002120"
                     /pseudo
     CDS             24591..25774
                     /gene="htrE"
                     /locus_tag="OO448_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6330 a         6984 c         6417 g         6043 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc ggtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc accacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgtg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctcg agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgtgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ataactatct aacg
//
LOCUS       DAJTVJ010000053        25576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000053 DAJTVJ010000000
VERSION     DAJTVJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.053"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(4..735)
                     /gene="rlmB"
                     /locus_tag="OO448_002121"
     CDS             complement(4..735)
                     /gene="rlmB"
                     /locus_tag="OO448_002121"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6337326.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(915..3356)
                     /gene="rnr"
                     /locus_tag="OO448_002122"
     CDS             complement(915..3356)
                     /gene="rnr"
                     /locus_tag="OO448_002122"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6337327.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3395..3820)
                     /gene="nsrR"
                     /locus_tag="OO448_002123"
     CDS             complement(3395..3820)
                     /gene="nsrR"
                     /locus_tag="OO448_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6337328.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4025..5323)
                     /gene="purA"
                     /locus_tag="OO448_002124"
     CDS             complement(4025..5323)
                     /gene="purA"
                     /locus_tag="OO448_002124"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6337329.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5427..5624)
                     /locus_tag="OO448_002125"
     CDS             complement(5427..5624)
                     /locus_tag="OO448_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6337330.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5706..6710)
                     /gene="hflC"
                     /locus_tag="OO448_002126"
     CDS             complement(5706..6710)
                     /gene="hflC"
                     /locus_tag="OO448_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6337331.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6713..7972)
                     /gene="hflK"
                     /locus_tag="OO448_002127"
     CDS             complement(6713..7972)
                     /gene="hflK"
                     /locus_tag="OO448_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6337332.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8058..9338)
                     /gene="hflX"
                     /locus_tag="OO448_002128"
     CDS             complement(8058..9338)
                     /gene="hflX"
                     /locus_tag="OO448_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6337333.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9415..9723)
                     /gene="hfq"
                     /locus_tag="OO448_002129"
     CDS             complement(9415..9723)
                     /gene="hfq"
                     /locus_tag="OO448_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6337334.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9809..10759)
                     /gene="miaA"
                     /locus_tag="OO448_002130"
     CDS             complement(9809..10759)
                     /gene="miaA"
                     /locus_tag="OO448_002130"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6337335.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10752..12599)
                     /gene="mutL"
                     /locus_tag="OO448_002131"
     CDS             complement(10752..12599)
                     /gene="mutL"
                     /locus_tag="OO448_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6337336.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12609..13946)
                     /gene="amiB"
                     /locus_tag="OO448_002132"
     CDS             complement(12609..13946)
                     /gene="amiB"
                     /locus_tag="OO448_002132"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6337337.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13965..14426)
                     /gene="tsaE"
                     /locus_tag="OO448_002133"
     CDS             complement(13965..14426)
                     /gene="tsaE"
                     /locus_tag="OO448_002133"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6337338.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14398..15945)
                     /gene="nnr"
                     /locus_tag="OO448_002134"
     CDS             complement(14398..15945)
                     /gene="nnr"
                     /locus_tag="OO448_002134"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6337339.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15944..17083
                     /gene="queG"
                     /locus_tag="OO448_002135"
     CDS             15944..17083
                     /gene="queG"
                     /locus_tag="OO448_002135"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6337340.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17066..17119)
                     /locus_tag="OO448_002136"
     CDS             complement(17066..17119)
                     /locus_tag="OO448_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337341.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17353..17428)
                     /locus_tag="OO448_002137"
     tRNA            complement(17353..17428)
                     /locus_tag="OO448_002137"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17393..17395),aa:Gly,seq:gcc)
     gene            complement(17464..17539)
                     /locus_tag="OO448_002138"
     tRNA            complement(17464..17539)
                     /locus_tag="OO448_002138"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17504..17506),aa:Gly,seq:gcc)
     gene            complement(17697..17772)
                     /locus_tag="OO448_002139"
     tRNA            complement(17697..17772)
                     /locus_tag="OO448_002139"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17737..17739),aa:Gly,seq:gcc)
     gene            complement(17983..18528)
                     /gene="orn"
                     /locus_tag="OO448_002140"
     CDS             complement(17983..18528)
                     /gene="orn"
                     /locus_tag="OO448_002140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6337342.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18623..19675
                     /gene="rsgA"
                     /locus_tag="OO448_002141"
     CDS             18623..19675
                     /gene="rsgA"
                     /locus_tag="OO448_002141"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6337343.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19772..20740
                     /gene="psd"
                     /locus_tag="OO448_002142"
     CDS             19772..20740
                     /gene="psd"
                     /locus_tag="OO448_002142"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6337344.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20762..24085
                     /gene="mscM"
                     /locus_tag="OO448_002143"
     CDS             20762..24085
                     /gene="mscM"
                     /locus_tag="OO448_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6337345.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24236..>25576)
                     /gene="yjeM"
                     /locus_tag="OO448_002144"
     CDS             complement(24236..>25576)
                     /gene="yjeM"
                     /locus_tag="OO448_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6337346.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5908 a         7177 c         6491 g         6000 t
ORIGIN      
        1 gaactagctg cgctgacgaa ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt
       61 cagggaagaa acacttcctg ccattgggat gctgatcaac tcatcgcaat gttcacgggt
      121 caggcgacgc ataccttcac cttccgcgcc catcaccagc gccaagcgtc cggtcatttt
      181 gctctgatag agtgtatgat ccgcctcgcc tgccgtaccg acgatccaga tattctcttc
      241 ctgcaacata cgcatggtgc gcgcaaggtt agtcacccga atcagtggaa cgctttctgc
      301 cgcgccgcag gctacttttt tcgccgtagc gttgagctgt gcggagcgat ctttcggcac
      361 aatcaccgcg tgaacgccag cagcgtccgc gctacgcagg cacgcgccga ggttgtgcgg
      421 atcggttaca ccgtcgagga tcagcaggaa cggttgatcg agcgaagcga tcagatccgg
      481 cagatcgttt tcctgatact gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac
      541 ggcaccgtcg cttttctcgt cgagatattg gcggtttgcc aactggataa ccacgccctg
      601 agactcaagg gcgtgaatca gcggtaacag acgtttatct tcacggcctt ttaaaataaa
      661 gacttcctga aaacgttcag gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta
      721 aatcatttcg ctcattaatg tactcgttgt ttacgttttg cctgatgcac tacgtttatc
      781 cggcctacat gatctctgca atatattgaa tttgcgtgat tatgtaggcc ggataaggcg
      841 ttcacgccgc atccggcatg aacaaagcgc attttgtcag caatctaacc ctcttctttt
      901 aaagagggta ttgatcactc tgccactttt ttcttcgccg cacgcttcgc tttggtcgct
      961 gcggctattt tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc tttcttcgcc
     1021 gctttcggct tcgttttttt ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt
     1081 ttaccgacct gacgacgctt gccgcctttt ttgcctgcat cgcctttttt cgctttctcg
     1141 cgcgccgttt taccgacgtt gcgcggtgcg cgttcactgg agatcagact aaagtcgatt
     1201 ttgcgctcgt ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa
     1261 gtctggccgc tggattcccc catcaggcgt tgccctacct ggtcaaagcg atagtagtca
     1321 ttgtccagcg aagagacatg gaccagacca tcaatgaaca agtcgtccag acggacgaag
     1381 aagccaaagc cagtgacgct ggaaattacg cctttaaaga cgttacctac ctggtcgagc
     1441 atgaagtcac acttcagcca gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc
     1501 gccatcgaac agtgctgacc cagttgcaac atctcttcca tcgaataatg gtagccgccg
     1561 gtttcagtgg tgttgccctg atgcccctgc tctttcgcca gcagatactt aatggcgcgg
     1621 tgcagcgtca ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat
     1681 gccaggccaa agtgaccacg gttttctgga tcgtaaatcg cctgtttcat cgagcgcagc
     1741 agcatggttt gcagcatttc tgcatcagga cggtcggcaa ccgactccag cagttccgcg
     1801 tagtcacgcg gttccggctt gttaccaccc ggcagctcca gccccagctc cgccagcact
     1861 gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt
     1921 tctttcgctt tctcaacgaa acgcgccgcc gagatattcg ccagaatcat gcactcttca
     1981 attaatttgt gcgcgtcgtt acgctgggtc tgttcgatac gttcaatacg gcgttcagcg
     2041 ttgaaaatga acttcgcttc ttcgctctca aatgagatcc caccgcgttc ttcacgggct
     2101 ttatccagca ctttatagag gttatgcaac tcttcgagat gcttaaccag cgggacgtac
     2161 tgctcgcgca gatcctgatc gccctgcaga atatgccaga ctttggtgta ggtcagacgc
     2221 gcgtgagagc tcatcaccgc ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa
     2281 accgtcatct cgcacaccat acacaggcgg tctacctgcg ggttgagcga acacaggccg
     2341 ttagagagca cttccggcag catcgggata acctgcgaag ggaagtacac cgacgtgcca
     2401 cggttacgcg cttctctgtc cagcggcgtt ggcggacgca catagtagct gacgtcggca
     2461 atcgcgaccc ataaacgcca gccgccgccg cgttttttct cgcagtaaac tgcatcgtca
     2521 aagtcacggg cgtcttcgcc atcaatggtg accagcggta aatcgcgcaa atcgacacgg
     2581 cccgcttttg cttcttccgg cacttcttct ttcagccccg caacctgttg ctcaacagcc
     2641 tgcggccaga tatacggaat ttcatgggta cgcagagcga tatcaaccgc catgccggtg
     2701 cccatattgt cgcccagcac ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga
     2761 cgctgagtca gttcgactac gaccacaaag cccatccgcg cgcccatgat ctgatcgggc
     2821 gggattaaga tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct
     2881 tcggtaaagt agcgaccaac aatctggctg gtttttggca ccagtacgcg gacaatacgc
     2941 gcttcacgac gacctttacg gtcagcaccc agcggctgcg ccagcacctg atcgccatga
     3001 atacaggttt tcatctgctc gctggagaga tacaaatcat ctttacgccc ttcaacccgc
     3061 agaaagccgt agccatcacg gtggccaata acggtacctt tcaccaggtc gaggcgttcc
     3121 ggcagcgcat agcactgacg acgagtgaag accagttgac catcgcgctc catcgcgcgc
     3181 aggcggcgac gcaggccttc aagctgctct tcgccttcaa tgtgcagttc taccgccagc
     3241 tcatcacggc tggccggttt ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta
     3301 gggatgggat tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg tgacatctcg
     3361 gttcctccgt tgtcatctct gatgaagatt ttcgtcactc caccagcaat aatttataaa
     3421 gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac
     3481 tttgcacggc cttagaaagc gcctgtttca accgacaggc aggtgtaatg tggcaaaact
     3541 cactgctgca attcaccagc gataagggct ccagctcgcg caccacatca ccaatacgta
     3601 tcgcactcgc cggtttaccc aggcgaatgc cgccattttt tccacgaaca gcagtcacgt
     3661 agccggcacg actaagttga ttgattattt tgaccatatg attacgggag acgccgtaga
     3721 cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg caatgacgcc atgtagatca
     3781 gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac atcaacctca aaagggaaat
     3841 cgggaaaaaa tacatttata ttgatgataa accagccaca agctgtgtcg ctaatttatt
     3901 tcagttcggg gaaggaaaaa agcgaggatt taacactgtg tcggatagcg taatggctta
     3961 accgacacag caatgtatca cactgttagc ccggcaggca aaatatctgc caggcgtacc
     4021 agaattacgc gtcgaacgga tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag
     4081 agatgatatc gatcggcaca ccggtcagct cttcaatacg cttgatgtag ttcagcgccg
     4141 cctgcggcag gccgctacga tctttcacgc cgaaagtgga ttcagaccag cccggcatgg
     4201 tttcgtaaat cggctctaca cctttccagt cgtcagctgc cagcggagtg gtagccactt
     4261 cgcgaccatc cggcatacgg taagccacgc agagtttaac ctctttcagg ccatccagaa
     4321 cgtccagctt ggtcaggcaa aagccagaca gggagttcag ctgtaccgca cgacgaacgg
     4381 caacggtgtc cagccagccg gtacgacgac gacgacccgt agttgcgccg aattcgttac
     4441 cctgcttgca gaggaactcg ccagtttcat caaacagttc ggtcgggaac ggacctgcac
     4501 ctacacgagt tgagtaagct ttgaggatac ccagaacata atcaacataa cgcgggccca
     4561 ggccggaacc ggtcgccacg ccaccagcag tggtgttgga agaagttacg tacggataag
     4621 taccgtggtc gatatccagc agcgtaccct gcgcaccttc aaacatgacg aaatcgccac
     4681 gctgacgcgc ctggtcgagc aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg
     4741 caacagccat cgtatcatcc agaactttct ggtaatcaac cgcttcagct ttgtagtagt
     4801 taaccaactg gaagttgtga tattccatca cttctttcag tttttcagcg aaggtttctt
     4861 tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgctac tttatcttca taagcaggcc
     4921 cgataccacg accggtggtg ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat
     4981 ccagcgcaac gtgataatca aggatcagcg gacatgcttc agacagcagc agacgctcac
     5041 gaacggggat gccacggtct tccagttctt tcatctcttt catcagcgcg gccggagaca
     5101 gcacaacacc gttaccgatg atgctggtta cattctcgcg gagaatacct gatggaataa
     5161 gatggagaac ggttttttca ccgttgatta cgagagtatg gcctgcgttg tgaccgccct
     5221 ggtagcgtac aacatattta gcccgttcag tcagaagatc gacgatctta cctttacctt
     5281 cgtcacccca ttgggtgccc agtacgacga cgttgttacc catttttcaa aatcaccgtt
     5341 tgcttaaaaa tggattctac catcgctttt tcagagttac agcacttttt gcactcaaaa
     5401 atgacctcga tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat gtagtagacc
     5461 acaacgcccg caaccacaag tccaccgcca aaacgacgta aaatattatc gggcaaattg
     5521 gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc
     5581 agtaccaaaa ccagggcaag cgccagccag attgtcgaat tcattcatta tccttataga
     5641 aaaagaaaac caccgacatc cctgaggatg cggtggcttt attgacctgt accgcagtcg
     5701 ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg
     5761 ctcatgacca tcacgtcctg attgccagag aagctgttct cataagcacg caggctacgg
     5821 atgaatgcgt agaagtccgg atctttactg aatgcatcag caaacagttt ggctgcttcg
     5881 gcatcgcctt caccacgcat gatgcggccc tgacgctcag cttctgccag cgttctggtc
     5941 acttcatagt cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg tgaacggtga
     6001 cgacgcgcta ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc agacacttcg
     6061 gtcggcaggt tgatctgctt gatacgcaca tcgacaactt caatacccag cgccgccata
     6121 ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg ctctgccgct
     6181 tcggcaatgg cgttatctgc cgccggggta gtaacttcat cttctgtacc cgcagaaccg
     6241 gagttcagcg cgtcacgtac ttcgagggtc agacgaccac gggaatcggt gacgatgtct
     6301 ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag agaacttacg tttcaacagc
     6361 acttccgctt gcgaaatgtc gccaccaccc gttgccaggt agtaacggct gaaatcgctg
     6421 atgcgccatt tgatgtaaga atcgacgatc aggtctttct tctctttggt cacaaagcgg
     6481 tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac
     6541 ggtatcttga aatgcagacc cggctcataa accagaggtt tgttgtcatc gtcacgcagt
     6601 accttaccaa aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa gacagacatg
     6661 taaagcacta ccagcacgat gatgataatc gcgataactg acttacgcat cgttattccc
     6721 cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg ttggtccata atatcgccct
     6781 gactggtgga cgacgtgttg cttgctccac tggttgtgga ggaagaggct ggcggcagac
     6841 gcagcagatt gctggcaccg ttatcgctct tcgccgcagg ggcgttaccg cctttcagca
     6901 tctggtctaa cggcagaacc atcaggttgc cacctttatc gttaaccagc actttgcggg
     6961 tgttacccaa cactttttcc atcgtctcga tatacagacg ctcgcgagta atttccggcg
     7021 cggctttata ttccggcaga agtttagcaa agcgcgccac ttcaccctga gcttccagga
     7081 tggtctgggc cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac
     7141 gcggctgaac ttcgttggta tacgcttctg cttcacgaat gtattgctgt tcgttttcac
     7201 gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc cggcggacga gctgcctgga
     7261 agttgacgtc cagcagagtg atacccatgt catacggacg gatagtctct tccagttcgc
     7321 gctgagtatc gctacgaatc acggtacgac cttccgtcag aatgcggtcc atggtgtatt
     7381 taccgataac tccacgcagg gcgctgtcgg tagcctgacg caggctgtca tccgggctgg
     7441 tcacgctata cagatatttt tccggattgg tgacgcggta ctgcacgttc atctcaacgc
     7501 gcactacgtt ctcgtccgac gtcagcatca caccagaagc ggccagttca cgcacggctt
     7561 ccacgttcac cggtttgact tcgtcgataa acgtcggttt ccagttcaga cccggctcaa
     7621 ccagatggct gaatttacca aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat
     7681 agaaaccact ggccgcccag ataatgacaa tcgctgccgc tgcgatggta acgacacgac
     7741 cgccaagctg cgggcgcggg ccttgcgatg aactgccacc gccagatccg gtgcctttac
     7801 cgccgcccag accaccgagc tttttgctca gtttgcggaa gatatcatct aaatcaggtg
     7861 gcccttgatc gcgaccgcct ttgtttccat ttccctcaga gttgccgcca ggtttgctgc
     7921 ttccccacgg gtcgcggtct tgtccgttat taccgggctg attccacgcc atgtttgtgc
     7981 tccatatttg ttatgcggtg atccctgttg tctttcgacg ccagaggata tgactccacg
     8041 cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg tttacagagg
     8101 cgacgccagt caacgatcgg catacgaact tgcagactta cgctgccgtc ctcctccatc
     8161 cactcttttt ctattgcctg aagctgataa aaacgacttc tcagacgtcc ttcctgcggc
     8221 ggcaaacgca atgtatgctg cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa
     8281 agctgtggta tccccgctcc ggtctgtgcg gaaagccaga cacggatcgg tttgttctct
     8341 tcgtcccgat caatacgcgg ttcgaaatct tccagcatat cgatcttgtt catcaccagc
     8401 agggttggga tctcgtgagc gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt
     8461 tcttgtacac gtacatccgc cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc
     8521 tcttgtaacg tggctttaaa tgctgccacc agatcgtgcg gcaggtggcg aataaaccct
     8581 acggtatctg caagtacggt ttcaccgaca tccgcgacgt caatacgccg caacgtcggg
     8641 tcgagggtgg caaacaactg gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa
     8701 agggtagatt taccggcgtt ggtatatccc accagcgaaa cagtaggaac gtcggctttg
     8761 atacgcgatt gccgcccctg ctcacgctgc ttttcaactc tttctaggcg cgactgtatc
     8821 tgcacgatgc gattacgcaa caaacgacgg tcggtttcga gctgggtttc acccggacca
     8881 cgcaaaccta tcccgccttt ctgtctttca aggtgggtcc agccacgcac caggcgcgta
     8941 gccagatggc gcagctgcgc tagctcaacc tgcaacttac cctcatgggt acgcgcacgt
     9001 tgggcgaaaa tatctaaaat aaggccggtg cggtcgataa cacgacactc gcacaaacgc
     9061 tccaggttac gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc
     9121 gtcgctttga cagcttccgc aatttcaact gctttacctt cacctacaaa atactttggg
     9181 tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc
     9241 agagattcaa actcctggag gtcttccata tctttgtctt gcgtaaaata gatgtgtacc
     9301 agtaccgcct gctcaccagc atcataacgg tcaaacaagc gtataaccct ctaaatagat
     9361 cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa aacagcccga aaccttattc
     9421 ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat gatggtagtt
     9481 actgctggta ccgccaccgg cgttgttact gtgatgagaa accgggcgag acgggacaac
     9541 agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa
     9601 ctgatcaaaa gactcgattt gcccttgcag cttaatacca ttcaccaaat aaatagaaac
     9661 tggaacacgt tcccgacgca gtgcgttcag gaacggatct tgtaaagatt gccccttagc
     9721 cattctctct tttccttata tgcttatttg tactttgaac ctttcgattc tgaaaaaatt
     9781 gcgcacgata cgtctcaatt gtacacattc agcctgcgat agcaccaaca acctgtaata
     9841 cttcgtcacg cgcctgttct ggtttttcac tgtcaagcca gtgaacccct tcccaaccac
     9901 gcagccaggt tatctgccgc ttcgccaact gtctcgtggc gcaaacacct cgataaacca
     9961 tttcatcgta tgagatttcg ccttcaaggt aagaccacat ctggcgataa cccacgcaac
    10021 gaatggaagg caagtccgta tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt
    10081 caaaacctga agccaacatc tgatgaaaac gctgctcaat gcgttgatgg agcagttcac
    10141 ggctcgccgg ggcgatggcg aactgatgca cctgatacgg tagagcgtct cctgacgttt
    10201 gcgtcagttc cgttaaagtt ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc
    10261 tttgtggatc atttggatga atccttgctg ccgcaaccgg atctacctcc tgaagttgac
    10321 gatgcaatga ctcccaacct tgctctgccg cctgttgctc aattctggcc cgcacttccg
    10381 ggtctgccga cggtagcggc gacaaccctt ccagcaatgc cttgaaatac aacatcgtac
    10441 cgcccactaa cagtgggatc cgccccgccg cggtgatatc ggccatttcc gccagcgcat
    10501 cgcggcgaaa atcagcagcc gagtaagcct gcgacggatc gcgaatatcc agcaatcggt
    10561 gcggcgcggc gagtaactct tcagcgttcg gcttcgccgt cccgatatcc atccctttgt
    10621 aaataagggc agaatcaacg cttatcaact ctactggtaa aattttacgc agctcaatgg
    10681 ctaacgccgt tttaccggag gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg
    10741 ccttactgat atcactcatc tttcagggct tttatcgccg gatgtaaatc aacagattgt
    10801 aacagaccac ccggcggcgt tttcacaagt tgcggacata accgttccac gtccgccagc
    10861 agggttatgg cctgtgccat tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc
    10921 cactgcgcaa tattgccagg ttcgaatacg gactgcttcg ccaggtagcc tatcagttca
    10981 ggaatcaaga tttgtaaatt ttgttggcgt aagggtaaag gcacggccct gatggtcaca
    11041 tgctgtgcat ctgactggaa atcaataccc aattccgcca gggcagactg cgctttttct
    11101 aatgccgatt tttcttcgcc agaaactttt agccgcaacg gaataagcag cggctgagcg
    11161 caaactggcg cttcacccgg cgtcagttgt acctgacgca gccaacgttc tgccactggc
    11221 aaggctaaaa gtgaaatgtt gccgtcgcgc tccagcaacg cacagtcgga atggacgata
    11281 gtcagtaccc gaccaaaact ctgactgttc gccgcaagtg caggttcctg cggttccggc
    11341 gcttttaatt tttgcatcgg cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc
    11401 tgtttctggt agcctggctg cgcattcggc cagggggcag ccggacgact gcctgatgct
    11461 ggcgcaggag tgtagcgcgg agctaccggc tcacgagctg ccggttctgc aaagtgattg
    11521 cgccccgccg ccacgcggtt ttccggaatg gaacgcggtg caggttgggg ttcatcgtcc
    11581 agcggtagcg gcgtttccag ttgctgttgt agcacgctca gcacgccctg atagataaaa
    11641 tcatgcacca gacgcgactg atggaaacgc acttcgtgtt tggcggggtg cacgttgacg
    11701 tccacctgat gcggatcgat ctccagatac agcacaaatg ccggttgctg atcggccccc
    11761 agtttgtctt cgcaggcctg gcggatcgcg tgattgatca ggcgatcgcg catcatgcga
    11821 ccgttcacgt agcaatactg aatttctgcc agtgcgggcg tggtgtgatt tggatcggcc
    11881 acccagccgc gtagcgtgag atcgccgtgt tgccattcaa tcgccagcgc ttgttcaaga
    11941 aaagcggtgc cgcaaatcgc gcctaagcgc cgttcttttt gcccgccttc cggcactgcg
    12001 cggtactgac gcacaatttt accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc
    12061 agcgcaatgc ggcggatgat ctcatcaatg tggttaaatt cggttttctc ggtgcgcagg
    12121 aatttgcgcc gcgccggggt gttgtagaac agatccagca cctccagcgt cgtccccaca
    12181 ggatgcgccg ccggttttac cgtcacgttc atatcgcgcc cttcggcata ggcctgccag
    12241 gcttcctgct gttctgcggt gcgtgaagtg agcgtcaggc gggaaaccga actgatactc
    12301 gccagcgcct caccgcgaaa gcccaggctg ataatggctt cgagatcgtc cagagaggcg
    12361 attttactgg tggcatgacg agccagcgcc agcgccagct catctttttt gataccgcag
    12421 ccgttatcac gaatgcggat aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc
    12481 gtcgcacctg catcgaggct gttttccacc agttctttga ctaccgacgc aggtcgctcg
    12541 accacctcac ctgcggcaat ctggttcgcc agttgtggcg gtaagacctg aattggcatc
    12601 aatcgtcctt agtttggcag cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc
    12661 gttgccccct gcggcgcaga ttgcatcgga tgcgcaagga aataattgcg caggcctttg
    12721 taaatggctt ctgccagctg ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg
    12781 ttgttgctga taaaaccggt ttcgaccagt actgacggga tatccggcga acgcagaacg
    12841 ccgagactcg cgtgttctgg tcggcgttta tgtatttcgc caatgcgttg caactgactg
    12901 atcatactgg tcgctacatc ataccctacc cgctgggaat gaccgaactg taaatccagc
    12961 accgcctggc ttaaataggg gtcagactga ctgttcgcca gcacatcgcc cgccccaccc
    13021 agcagctccg actgtttctc gtgctgctcc agccagctgg ccatttcgct gttggcgcga
    13081 cggttagaga gcacccatac ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg
    13141 tgaatcgaca cgaggaaatt ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc
    13201 gagataaagt aatccccgtc acgggttaaa acgcctttaa acatcggatc gtcattgagc
    13261 aaagtgcgca atttacgcgc gatggcgatg gtgacatttt tctcccgcgt accaccgggg
    13321 ccgatagcgc cagggtcctg accgccgtgt ccggcatcaa tagcgatgat aattttatcg
    13381 ccagtgttag ccgtcgcgcg cgctgccgga cgcgttaccg tattactgct gataacaccc
    13441 gtagtgcggt tactttccgt tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg
    13501 acaaccgcag gcgtttcaac gcgtttcgca accacgggcg gtggaggagg cggtggcggc
    13561 acatcggcgt taatcgtaaa gacgacagtg taattgctgc cattctgccg cttcaccgct
    13621 tcggttttac cgttttcggt aagatcgacc accagccgta gcgtttgtgc atctttaggc
    13681 gttccagagc gaatcgcctt caccagatta ttgccgctga acaacaacgg cagtccctga
    13741 atcacgcccg tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca
    13801 taatcaggat cgccaataaa actcaacgtt atccgcgcct gttgattacc gttagaaacc
    13861 tgaatatcag agagcgtcgc ggcacccacc ggcgtgcaca gcagcagcag cgtcgctacc
    13921 aaccaatttc tgatgcgata catcatcccg ccacctttca aaggttaacc ggctaaacgc
    13981 gccagcaaca attcacccgc agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc
    14041 tgataatcaa tgtgtatttc gacatccggg tcaggaagaa cacctgtacc ttgttgtggc
    14101 cactccacca ggcagatggc atcgttggca aaataatcgc ggatccccat aaactccagc
    14161 tcctcgggat cggcaaggcg gtacaaatca aagtgataaa ccattaaatt gtcgagcgta
    14221 tagggttcga ccagcgtata agtggggctt ttgacattac cctgatgacc cagagcttgt
    14281 aaaaaacccc ggctaaaggt ggttttacct gcgcctaaat cgccatacag atagattacg
    14341 gttgcgccat cgcaggcttt cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca
    14401 gggagcggaa ttactcgatt catcatggtt tttatcagtc acttccgggt taacaatacg
    14461 ctgtagcgtg gaaaagagat cggttgccag catcccgcgc gttccaaaac gcgccgccag
    14521 tacgtcagct gccgcaccgt gcgcgacaca gcccgcacag gcggcatcat acggcgacag
    14581 tttttgccca agcaatgcgc caataatacc agagagcaca tcgcccatgc cgccgctcgc
    14641 catgcctgca tttccggcat caataatgcc taaagcgtca ggatgggcgg cgaccacggt
    14701 tccggcacct ttcagcaccg ctacgccgcc ataacgttgt accagacgtt tggcgcaatg
    14761 taagcggtca ctttcaattt cagcgacgga acagcctaac aaccgtgcgg cctcgccagg
    14821 atgcggcgtg atcacgcgat tgtgacgctt atcgggattg attgccagca ggttcaatgc
    14881 atcggcatcc cacaacatcg gtttgcgaaa attctcaact ttttgcagtg cttttttccc
    14941 ccactcttgc tggcccagac cgggaccaat caccaccaca tcggcccatt ccaggctttc
    15001 ggtaagagag tccatcgtca gttcatgcac catcaattcc ggtcgtgcag tcagcagcgg
    15061 cgcaatgttt tcactgcggg tcagtactcg gactaaacca gcaccagcac gcagcgccgc
    15121 ttcccccgtc atacgaatag cccccgccgt gccgtgatcg ccaccgataa tcaccagccg
    15181 cccgtgatcg cctttatgcg aagtcgggcg acgcggtttc agccagtgag aaagttgttc
    15241 tgccgaaaac cgctgaattt tcgtctcctg acctgccagc cagctatcca gccccagtga
    15301 gtcaaaatgc agttgtcctg taacatcccg cgcttttcca gtaagcaagc ctggtttcag
    15361 cgcaataaaa gtgatggtgt gatcggcgtt gatcactgcg cctggcgtag cgccggtttc
    15421 agccagcagg ccggaaggga tatcaaccgc cacaatcggc gcaggatggg aattagcgtg
    15481 gtcgattaac tggctaatgg attcgcgggg cgcttgctgc aagccggtac cgagcagcgc
    15541 atcaacaatc agatctaccg attcgggcca gacaatattc gaagcatgga tctcgccacc
    15601 cgcgtttaac catgcttcgc gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc
    15661 ctgggccaac aacgtgacct caatgccgac cgctttggcc agtcgcgcga ccacgtagcc
    15721 atcgccgccg ttattaccat gaccgcacag caccagccag tggcgggcgt caggatacgc
    15781 cgaacgacac acctggaatg cggcctcgcc agcgcgaagc atcagctcat agagtgtgag
    15841 ccccagcaca tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt accagacggt
    15901 gtgtggtata cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga
    15961 tctcaatcag ttagcgcaaa aaattaaaca gtgggggctg gaactgggct ttcagcaggt
    16021 aggtattacc gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa
    16081 acaataccac ggcgaaatgg actggatggc acgtcacggt atgctgcgcg ctcgccccca
    16141 tgagttatta ccaggtacgc tgcgtgtaat cagcgtgcgg atgaattacc ttcctgctaa
    16201 cgccgcattt gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct
    16261 gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa aagctgggcg agatgattca
    16321 gcaacattgt gtttcgctga attttagacc gtttgtcgat tctgcgccta ttctcgagcg
    16381 cccgttagct gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg
    16441 cgaggccggt tctttcttct ttttaggcga attgctggtc gatattccgc tgcccgtgga
    16501 tcaaccagtc gaggaaggat gcggtaaatg catagcctgt atgacgattt gcccgaccgg
    16561 tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga
    16621 acttgaaggg gcgatcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg
    16681 cgatgactgc cagcttatct gcccgtggaa tcgctattcg caactcacta cagaagacga
    16741 tttcagcccg cgtaagccgc tacacgcaca ggaactcatt gagttatttg cctggagcga
    16801 agagaagttt ttaaaagtca cggaaggatc ggcgattcgc cgtataggtc acctgcgctg
    16861 gctgcgtaat atcgccgtgg cattaggcaa tgcgccctgg gatgaaacga ttctggcggc
    16921 gctggagagt cgcaaaggtg agcacccact tcttgatgag cacatagcgt gggcgattgc
    16981 gcagcaaatc gagagacgaa atgcgtgcat agtcgaagtg caattgccga aaaaacagcg
    17041 tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg
    17101 aataaaaata aaaacgcatt gcaattcaag acgcataaaa tcgtcaagtg atcgactcga
    17161 caaaacggaa tcttgatttt taacttattt atcaataggt taaaaaaata ccattcaccg
    17221 tgcgaagttt ctttgaaatt aagattacag cctgaggatg tggataactc tgtttacaag
    17281 agtttgtcag agacaacaaa aaccagcaat aacgcatcgc gtcgctgtgg atattttatt
    17341 gagagaagaa tttggagcgg gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt
    17401 cgtgctctac caactgagct attcccgctt gggtggtctg tgccttacag cactttcaaa
    17461 ttttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta
    17521 ccaactgagc tattcccgct taatcttcgt ctttcgaacc gctactgcct tggctgcctt
    17581 cactcacctt agtcacatac ttttgtaagt tcccaaggat tcgttcagtt gccgccttgt
    17641 cgcaattcga aatactgcga ttgggtggtc tgtgctttgc agcacttttc aaattttgga
    17701 gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg
    17761 agctattccc gcgtactact taattttgct ttcgtaattt tcgcatttct gcgtcgttac
    17821 gggaggcgca ttatacgaga aatctttccc tctgcaaccc cccaaaaaag ttttttttgc
    17881 gtttttatct caagtgctga aattatcagc aagacgttca atttagcgtc aaaatcacgc
    17941 cgccgcaagt ggattccggc gcttacgtga ccaggaaaaa ttttacagct tgataaaatg
    18001 ctcgcggtag taagccagct ccgccaccga ttcacggata tcatccattg cctgatgcgt
    18061 cccctgcttg gtaaaaccat ccagaatttc cggcttccag cggcgcgcca gctctttcag
    18121 ggtgctgaca tcgagataac ggtagtggaa gtaggcttcc agctccggca tgtatttaaa
    18181 caggaaacga cggtcctgtc cgatgctgtt accgcaaatc ggcgattttc ccgcaggcac
    18241 ccactgtttt aagaattcga gcgttgccag ttcagcttcc cgatcgccca tcgtgctcgc
    18301 tttcacgcgc tctaccagcc cgctggcggt atgggtgcgc acgttccagt catccatcag
    18361 cgccagctgt tcatcggact gatgtactgc aatggtcggc ccttctgcca gaatattcag
    18421 attggcatcg gtcaccagcg tggcaatctc aataatgcga tcgcgctcgg gatccagacc
    18481 ggtcatctca agatcgatcc aaatcaggtt gttttcattg gcactcatgc tattttccac
    18541 ccattcacag tgactatatt gatccacaat tgcgtgtatc atagatgttt tgcccatcag
    18601 gggcgaccag gagtcagtac gattgagtaa aaataaactc tccaaaggcc agcagcgccg
    18661 cgtgaacgcc aatcaccagc gtcgtcttaa aacgtctaag gagaagcccg actacgacga
    18721 caatctgttt ggcgagcctg atgaaggtat cgtcatcagc cgctttggta tgcacgctga
    18781 tgtggaatcc gccgatggcg acgttcaccg ctgcaatatt cgccgtacca tccgttcgct
    18841 ggtaaccggc gaccgcgtag tctggcgtcc gggtaaaccg gcggcggaag gcgtaaatgt
    18901 caaagggatc gtggaagcgg tgcatgagcg tacctcggtg ttgacgcgtc cggatttcta
    18961 cgacggcgta aaacctattg ccgccaacat cgaccagatt gtcattgtct ccgccatttt
    19021 gccggagctg tcgctcaata ttatcgaccg ttacctagtg gcctgcgaaa ccttgcagat
    19081 tgagccgatt attgtgctca acaagataga cctgctggac gacgaaggca tggcgttcgt
    19141 caacgagcag atggatatct accgcaatat cggttatcgc gtattgatgg tttccagcca
    19201 tactcaggat gggctaaaac cgctggaaga ggcgttgacc gggcgcatca gcatttttgc
    19261 cgggcagtct ggcgtcggca aatccagcct gctgaacgcg ttgctggggc tgcaaaaaga
    19321 gatcctgacc aacgatgtct ctgacaactc ggggcttggt cagcacacca cgaccgccgc
    19381 tcggctgtat cacttcccgc acggcggtga tgtgattgat tcccctggtg tgcgtgagtt
    19441 cggcctctgg cacctggagc cggaacaaat cactcagggc tttgtcgaat tccatgacta
    19501 tttaggtctg tgtaaatatc gcgattgcaa acacgatacc gatccgggct gcgctatccg
    19561 ggaagcggtt gaggaaggga aaatcgcgga aacccgtttc gaaaactatc accgtattct
    19621 ggaaagcatg gcgcaggtaa aaacgcgtaa aaacttttct gatacggatg actgacaatt
    19681 aagctaaccg tcgttagaat cgtccccttt ttttcaggat ccggcatgta ggccgaatca
    19741 ggaacgacaa aacaatggcc tggaggctac cttgttaaat tcatttaaac tttcgctaca
    19801 gtacattctg ccgaaactat ggcttactcg cctggcgggt tggggcgcaa gcaagcgggc
    19861 aggatggttg acaaaactgg ttatcgatct gttcgttaaa tactacaagg tcgacatgaa
    19921 agaggcgcaa aagccggaca ccgccagcta ccgcaccttt aacgaattct ttgtccgtcc
    19981 gctgcgtgac gaagtacgcc caatcgatac cgatccgaat gtactggtca tgcctgccga
    20041 tggcgttatc agccagttgg gtaaaatcga agaagataaa atcctgcaag ccaaaggcca
    20101 caactacagc ctcgaagccc tgctggcagg caactatctg atggcggacc tgttccgcaa
    20161 cggtacgttt gtgaccactt acctctcccc gcgtgactac caccgcgtac acatgccgtg
    20221 caacggtatt ctgcgtgaga tgatctacgt gccgggcgat ctcttctccg ttaaccatct
    20281 cacggctcag aacgtgccga atctgtttgc ccgtaacgaa cgcgtgattt gccttttcga
    20341 taccgaattt ggcccaatgg cgcagattct ggtcggagcg acgattgttg gcagcattga
    20401 gacggtctgg gcgggcacca ttacgccgcc gcgcgaaggt atcatcaagc gctggacctg
    20461 gcctgccggg gaaaacgacg gttctgtggc actgctgaaa ggccaggaaa tgggtcgctt
    20521 taaactcggt tccaccgtta tcaacctgtt tgcaccgggt aaagtgaatc tggttgagca
    20581 actggaaagc ctgtctgtca cgaaaatttg ccagccgctg gcagtatcta ccgaaacctt
    20641 tgttacgcca gacgctgaac ctgccccgct tcctgctgaa gagatcgaag cagaacacga
    20701 cgccagccca ttggttgacg acaaaaaaga ccaggtctaa tccatcaaag gaaacgctga
    20761 cgtgcgcctg attatcactt ttctgatggc ctggtgcctc agttgggggg cgtacgccgc
    20821 gacggccccc gatagcaaac aaatcactca ggaactggag caggcaaaag cggcgaaacc
    20881 cgcacagccg gaagtcgtag aggcgcttca gtctgcctta aatgcgcttg aggaacgaaa
    20941 aggttccctt gagcgcatca aacaatatca gcaagttatc gataattatc cgaaactctc
    21001 cgctactctg cgcgcacaat taaacaacat gcgtgacaag ccgcgcagcg tgtcgccggg
    21061 aatgtctacc gacgcgctga atcaggaaat tctccaggtc agcagccagt tgctggataa
    21121 aagccgtcag gcccagcaag agcaggagcg cgcccgcgag attgccgatt cgctgaatca
    21181 actgccgcaa cagcaaaccg acgcccgccg tcagttaaat gagatcgagc gccgcctggg
    21241 aacgcttacc ggcaatactc cgctcaatca ggcacaaaat ttcgcgttgc agtctgactc
    21301 tgcacgtctt aaggcgctcg ttgatgaact ggagctggcg cagctgtctg ccaataaccg
    21361 ccaagaatta gcgcgcttac gctcagagct tgcggaaaaa gagagccagc aactggatgc
    21421 gtatttgcag gccttacgta atcagttgaa tagccaacgt cagcttgagg cggagcgggc
    21481 gctggaaagt accgaattac tggcagaaaa cagtgccgat ttgccgaaag atatcgtcgc
    21541 gcaattcaaa attaaccgcg aactatcggc agcgctgaat caacaggcgc agcggatgga
    21601 tctcgttgcc tcgcaacagc gtcaggctgc cagccagacg ttacaggtcc ggcaggcgtt
    21661 gaatacgctg cgtgaacagt cgcaatggct gggatcgtcc aatctgctcg gtgaagcgtt
    21721 gcgagcgcag gtggcacggc taccagaaat gccaaaacca caacagcttg ataccgaaat
    21781 ggcgcagttg cgtgtgcaac ggttacgtta tgaggatctg cttaataaac agccgctgct
    21841 acggcaaatc catcaggccg acggtcagcc gctgaccgcc gagcaaaacc gtattctgga
    21901 agcacaactg cgcactcagc gtgagttgct gaactcattg ttgcagggtg gcgacacgct
    21961 actattggaa ctgaccaagc tgaaagtctc caacgggcaa ctggaggatg cgctgaaaga
    22021 ggtgaacgaa gcaacgcacc gctatctgtt ctggacctct gacgtgcgtc cgatgaccat
    22081 cgcctggccg ctggaaatcg cccaggatct gcgtcgtctc atttcgctgg acaccttcag
    22141 tcagttgggc aaagccagtg tgatgatgct gaccagcaaa gagacaattt tgccgctgtt
    22201 tggcgcgttg attctggtcg gttgcagtat ttactcgcgc cgctatttca cccgttttct
    22261 tgaacgttcg gcggcgaaag ttggcaaagt gactcaggat cacttctggc tgacgttgcg
    22321 cactcttttc tggtcgattc tcgtcgcgtc accgttaccg gtgctgtgga tgacgctggg
    22381 ttacggcttg cgcgaggcgt ggccttatcc gctggcggtc gcgattggcg atggtgtaac
    22441 ggccaccgtg ccgctgctgt gggtagtgat gatttgcgcc acctttgccc gcccgaacgg
    22501 cttgtttatc gctcattttg gctggccgcg cgaacgcgta tctcgtggga tgcgctacta
    22561 cctgatgagc atcgggctta ttgtgccgct gattatggcg ctgatgatgt tcgataacct
    22621 cgacgaccgt gaattctccg gttcgctggg aaggctttgc tttatcctca tttgcggtgc
    22681 gctggcggtg gtcaccctca gcctgaaaaa ggccgggatt ccgctgtatc tcaacaaaga
    22741 aggcagcggc gacaacatta ccaaccatat gctgtggaac atgatgattg gcgcgccgtt
    22801 ggttgccatt ctggcgtcgg ctgtgggtta tctggcaacg gcacaggcgc tgttagcgag
    22861 gcttgaaacc tcggttgcca tctggttcct gctactggtg gtttatcacg ttatccgccg
    22921 ctggatgctg atccagcgcc gcaggctggc gtttgaccgg gcgaagcatc gccgggcaga
    22981 gatgttagcg caacgtgcgc gtggcgaaga ggaagcgcat catcacagta gcccggaagg
    23041 agcaattgaa gtcgatgaaa gcgaagtcga tctcgatgcc atcagtgcgc aatccttgcg
    23101 gctggtgcgc tcaattttga tgttgatcgc cctgctttct gtcattgtgc tgtggtcaga
    23161 aatccattcc gctttcggct tcctcgaaaa tatttcgctg tgggatgtca cctccacggt
    23221 acagggcgta gaaagtctgg agccaattac cctcggtgcg gtgctgattg ccattctggt
    23281 gtttatcatc accacgcagc tggtgcgcaa cctgcccgcg ctgctggaac tggcgatttt
    23341 gcagcacctg gatttaacgc cgggtacggg ctacgccatc accaccatca ccaaatatct
    23401 gctgatgctg attggcgggc tggtcggctt ctcgatgatt ggtattgagt ggtcgaaatt
    23461 gcagtggctg gtcgccgcgc tcggtgttgg tctcggtttt ggtttgcagg aaattttcgc
    23521 caactttatc tctggtctga ttatcctgtt cgaaaaaccg attcgcattg gcgatacggt
    23581 gacaattcgc gatctcaccg gtagcgtgac gaaaattaac acccgcgcca ccaccatcag
    23641 cgactgggac cgtaaagaga taatcgtgcc gaacaaggcg tttattaccg agcagtttat
    23701 caactggtcg ctctctgact cggtcacgcg cgtagtgttg acgattccgg cccctgctga
    23761 tgccaacagt gaagaagtga cggaaatcct gctcaccgca gcgcgtcgct gctcgctggt
    23821 gatcgacaac ccggcaccgg aagtcttcct ggtggatctg caacaaggga ttcagatttt
    23881 cgagctgcgt atttatgccg ctgagatggg tcaccgtatg ccgctacgcc atgagatcca
    23941 ccagctgatt ctggctggct tccatgccca cggtatcgat atgccattcc cgcccttcca
    24001 gatgcgtctg gaaagcctca acggtaaaca aacggggaga acgctgacgt ctgcgggcaa
    24061 aggtcgtcag gcgggaagtt tgtaatgcat gaagctgcca aatgatgtca acgtggctca
    24121 tttagcagct taacatcatc ttcagttatc accggccctg gagagtatca tccatacgca
    24181 ttacttcctg acaactcatc gccggagaga cgatgagttg caggatttca tatgatcagt
    24241 cgagagctaa ctcaggttta ttcgccatgc gactgcaata gttctggtaa atcgccatcg
    24301 ctaacagcga gaagaagaca gggccgccaa tcatccacaa tgtgctgtcc cagtctccgg
    24361 cttcaaccac aggctgaata atggtgaaga cgttcgcaaa tgtcaccacc agtacgacaa
    24421 ccaccatcgc aatcattgcc gacatacgcg ttttgaaaat cacaaatggt ctgtcgagat
    24481 cctgacgtgc tttaaagaat gggaaagcca gcgcgaggaa caggtaagga agcgtcatag
    24541 acacgttcgc catcagcgtc agcttgttaa agaacgcgga tgcggtaccg ccaccaaacg
    24601 aaaccagcag gatgaagaca gtaaccaacc cgcactgcat ccacatggcg atagacggca
    24661 tccccatcgc attcaggcgc gtcatcggtt ccggccacaa cgctttcggc gtcccctgga
    24721 tgatggcttt cagcggtgaa tagcaaagcg taaagaacgc accggtataa gcgaggaaca
    24781 tcgaaagtcc ggtaatacgc gcaaaccata cgcccagcga caatgacgct tcaggtgaca
    24841 aatgcagtgc gttacccagc gtcaccccga ggctcttcat cagcacatag gtaatattgc
    24901 caaggttaac ggaaccatta cttaatacct gctgccagtt tgtgctgacg ccccataaaa
    24961 atattgccag cgaataaccg attgaaataa caatagcggc aaaaacaata cctttggcaa
    25021 agttcttttc tggattttcc gttttatcga ccagaccacc aaccgcttca attccgccat
    25081 aggcaaaaat agcaaatacc acaaacgata gcattgccag accggactga taacccgggt
    25141 tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc accatttaat aacaaaatag
    25201 taatgcttac taacagcaat actaaattca gacacattac tgcaataccg cccaccgcag
    25261 taatgcgggc aattttatta atcccctttg aagcaacgac ggtgaccaga atcatccatg
    25321 ctactgccag cagaccaacc acctgcgtag gctccagtcc ggcaatacgc cagtgctggg
    25381 tcatgtcgct accatagagg aatgttgaga acggtaccca aactttcgcg gaggtactca
    25441 ccatccagat gatataagag gaaaaccaca taaacgtacc aatgaaggca aaacgtggtc
    25501 cgacactatt attcatccag gaatagatac caccttcttc tttgcgataa gcagctccca
    25561 tttcagccat cattaa
//
LOCUS       DAJTVJ010000054        25529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000054 DAJTVJ010000000
VERSION     DAJTVJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.054"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            15..485
                     /locus_tag="OO448_002145"
     CDS             15..485
                     /locus_tag="OO448_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR6337347.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            487..906
                     /gene="gspB"
                     /locus_tag="OO448_002146"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="OO448_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCR6337348.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="OO448_002147"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="OO448_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6337349.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="OO448_002148"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="OO448_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6337350.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="OO448_002149"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="OO448_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6337351.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="OO448_002150"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="OO448_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6337352.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="OO448_002151"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="OO448_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6337353.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="OO448_002152"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="OO448_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6337354.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="OO448_002153"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="OO448_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6337355.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="OO448_002154"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="OO448_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6337356.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="OO448_002155"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="OO448_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6337357.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="OO448_002156"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="OO448_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6337358.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="OO448_002157"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="OO448_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6337359.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="OO448_002158"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="OO448_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6337360.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="OO448_002159"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="OO448_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6337361.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="OO448_002160"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="OO448_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6337362.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="OO448_002161"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="OO448_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6337363.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="OO448_002162"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="OO448_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6337364.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="OO448_002163"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="OO448_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6337365.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="OO448_002164"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="OO448_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6337366.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="OO448_002165"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="OO448_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6337367.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="OO448_002166"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="OO448_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6337368.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="OO448_002167"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="OO448_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6337369.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="OO448_002168"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="OO448_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6337370.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="OO448_002169"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="OO448_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6337371.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="OO448_002170"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="OO448_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6337372.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="OO448_002171"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="OO448_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6337373.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="OO448_002172"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="OO448_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6337374.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="OO448_002173"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="OO448_002173"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6337375.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="OO448_002174"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="OO448_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6337376.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="OO448_002175"
     CDS             14905..15273
                     /locus_tag="OO448_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR6337377.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="OO448_002176"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="OO448_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR6337378.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="OO448_002177"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="OO448_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR6337379.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="OO448_002178"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="OO448_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR6337380.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="OO448_002179"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="OO448_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6337381.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="OO448_002180"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="OO448_002180"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6337382.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="OO448_002181"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="OO448_002181"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6337383.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="OO448_002182"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="OO448_002182"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6337384.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="OO448_002183"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="OO448_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6337385.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /locus_tag="OO448_002184"
     CDS             21950..22423
                     /locus_tag="OO448_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6337386.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="OO448_002185"
     CDS             22452..22994
                     /locus_tag="OO448_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR6337387.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="OO448_002186"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="OO448_002186"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6337388.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="OO448_002187"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="OO448_002187"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6337389.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="OO448_002188"
     CDS             24391..24648
                     /locus_tag="OO448_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6337390.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="OO448_002189"
     CDS             complement(24624..25178)
                     /locus_tag="OO448_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6337391.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6501 a         6033 c         6684 g         6311 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgctt gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagccagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacttggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaagccgcgt
    25501 cgcaactgct ctttaacaat ttatcagac
//
LOCUS       DAJTVJ010000055        25428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000055 DAJTVJ010000000
VERSION     DAJTVJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.055"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="OO448_002190"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="OO448_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6337392.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OO448_002191"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="OO448_002191"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6337393.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="OO448_002192"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="OO448_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OO448_002193"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="OO448_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6337394.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="OO448_002194"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="OO448_002194"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6337395.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="OO448_002195"
     CDS             complement(6490..6846)
                     /locus_tag="OO448_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6337396.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="OO448_002196"
     CDS             complement(6850..7266)
                     /locus_tag="OO448_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6337397.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OO448_002197"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="OO448_002197"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6337398.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8714
                     /locus_tag="OO448_002198"
                     /pseudo
     CDS             8492..8714
                     /locus_tag="OO448_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OO448_002199"
     CDS             complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="OO448_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6337399.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OO448_002200"
     CDS             complement(10664..11743)
                     /gene="alr"
                     /locus_tag="OO448_002200"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6337400.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OO448_002201"
     CDS             complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="OO448_002201"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6337401.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13294..14277
                     /locus_tag="OO448_002202"
     CDS             13294..14277
                     /locus_tag="OO448_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6337402.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OO448_002203"
     CDS             complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="OO448_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6337403.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OO448_002204"
     CDS             complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="OO448_002204"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6337404.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16219..17204)
                     /locus_tag="OO448_002205"
                     /pseudo
     CDS             complement(16219..17204)
                     /locus_tag="OO448_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17522..18037
                     /gene="zur"
                     /locus_tag="OO448_002206"
     CDS             17522..18037
                     /gene="zur"
                     /locus_tag="OO448_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6337405.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18079..18288)
                     /locus_tag="OO448_002207"
     CDS             complement(18079..18288)
                     /locus_tag="OO448_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6337406.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OO448_002208"
     CDS             complement(18404..19729)
                     /gene="dinF"
                     /locus_tag="OO448_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6337407.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OO448_002209"
     CDS             complement(19802..20410)
                     /gene="lexA"
                     /locus_tag="OO448_002209"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6337408.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OO448_002210"
     CDS             complement(20520..20888)
                     /gene="dgkA"
                     /locus_tag="OO448_002210"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6337409.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21059..23482
                     /gene="plsB"
                     /locus_tag="OO448_002211"
     CDS             21059..23482
                     /gene="plsB"
                     /locus_tag="OO448_002211"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6337410.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OO448_002212"
     CDS             complement(23637..24509)
                     /gene="ubiA"
                     /locus_tag="OO448_002212"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6337411.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24521..25018)
                     /gene="ubiC"
                     /locus_tag="OO448_002213"
     CDS             complement(24521..25018)
                     /gene="ubiC"
                     /locus_tag="OO448_002213"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6337412.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25241..>25428)
                     /locus_tag="OO448_002214"
     CDS             complement(25241..>25428)
                     /locus_tag="OO448_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337413.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
BASE COUNT         6580 a         6542 c         6088 g         6218 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgctaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     8641 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     8701 atttatcgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     8761 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     8821 tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagt tctctttaac
     8881 attagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata aggcaaagta
     8941 tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt attaaattta
     9001 ataatggtta gatgtgcaga aattattact ccacacgaaa aaatagggaa aggaattcca
     9061 gataattggg ataaacttaa caataaaaga actgacaacg ttttattatc tctcacgtag
     9121 aacaacggca tcaaaaaaat catctacaaa gaaatagtga aagcgacgac agtttaattt
     9181 cactgcaggc tgggtaactc cagcctgctt tcctgcatta catcaccgca gcaaacgcct
     9241 ttgccacacg ttgtacattt gccgtattta acccggcgac acacatgcga ccgctggcga
     9301 tgagatagac accaaattct tcacgtagtc ggtcaacctg agcggcactt aaaccggtat
     9361 aactgaacat gccgcgctga ttaagcagat aatcgaaatt gcgttctggc atctctgtgc
     9421 ttaatacctt caccaattcc tgacgcattg ccagaatgcg agtacgcatc tcttctactt
     9481 ccgccagcca gctggctttc aatgcctcgt cattcagcac tgcagccacc acctgcgcac
     9541 caaaattcgg cgggctggag tagttgcggc gaactgttgc tttcaattgc cccagtacgc
     9601 ggccagcggc ttcggcatct tcacacagaa cagaaagtcc gccgacgcgc tcgccgtaaa
     9661 gggagaaaat tttcgagaac gaattgctca ccagagcggg taatccagcg ctggcaatgg
     9721 cgcgaatagc gtaggcatcc tcttccatac cggcactaaa tccttgatag gcaatatcga
     9781 ggaatgggat aagctcgcgg gctttgagaa tttcaatcac cgcatcccac tgatcattag
     9841 tgagatcggc acccgttggg ttgtggcaac atggatgcag caacacaata ctgcgggcag
     9901 gtaatgtttt cagcgtcgcc aacaggtcat taaagcgcac gctgttagtc gcttcgtcat
     9961 accaggggta agtactcact tcgaatccag ccccggcgaa tattgctacg tggttttccc
    10021 aggtaggatc gctgacccag acgcctgatt ccgggaagta gcgtttcagg aaatccgcgc
    10081 ccactttcaa tgcccctgag ccgccaaggg tttgaatggt tgctacgcgc tgttgttgca
    10141 gtaccggatg gtccgcacca aacagcagcg gcgcaatggc atggcgatag ctgttaagcc
    10201 cttccatcgg taaataaagc gaagcgccat ggggctgcgc attcaggcgc gcttccgcct
    10261 ccgccacggc tttcagttgt ggaataattc cgtcttcgtt gtagtacaga ccgatactta
    10321 aattcacttt gtcgctgcga gggtcttctt taaaacgctc cataagcgta agaatcggat
    10381 cgccagcgta ggcgtcaact ttttgaaaca cgcgatggtt ctccaggttt acgggcaggt
    10441 ggttaaaaca caataaaccg gaagaaggcg aagatcgagt ggatgttcag gagcgaacgg
    10501 caattagcaa cagagtgaga ctcatgacaa acgaacatcc gccagaggca cgacctttat
    10561 aagagcatga aagaacatca acttattgaa tttttaggat tttattggcc ggataaggca
    10621 ttcacgccgc atccggcaca gacaatcaaa tattacagaa cgattaatcc acgtatttca
    10681 tcgcgaccct tgaagtcagg cgcgtaataa gttcgtaagc gctcactttt gtcatttcag
    10741 cgatacgttc tacgggcaaa ccttcgcccc ataaaatgac cggatccccg gctttgtcct
    10801 gcgcctgtgg acctaagtct acgcagatca tatccatcgc cacgcgtccg acaatcggta
    10861 cttcgcgacc gttcaccagc actggcgtac cggatggagc ggcgcgcgga taaccatcgc
    10921 catagcccat cgcgactacg ccaagacggg tatcacgttc gcttacccag gttccaccat
    10981 aaccaacagg ctctccggct ttatgctcac gcacggcaat caggctggag gttagtgaca
    11041 tcactggctg acagccaaaa tcggcaccga tggagcgatc ttccagcggc gagacgccat
    11101 aaagaatgat gcccgggcgc acccagtcaa aatgcgattg tggccacagc agaatgccac
    11161 ccgacgcggc aatggaacgt tgaccaggtt tgccttcgca aaaggtatta aagatagcga
    11221 gttgtttctc ggttgcgcca cattttggtt catccgcgcg cgcaaaatgg ctgacgatat
    11281 tcaccggctg acgaacgttt ttgcactgtg tcaggcgatg ataaaacgcc tcagcctgtt
    11341 ccggccttac gcccagacgg tgcataccgg tatcgagttt catccagacg gtaaccggct
    11401 cgtccaggct agcctcttcc agcgcagcca gctgttcttc gttatgcacg gcggtatgaa
    11461 aatgttgcgc agaaatcgtc ggcagatctc tggcatcaaa aaagccttcg agtaacagta
    11521 caggtttggt gattcccccc gcacgcagtc gcagagcttc ttcgagacgg gctacgccaa
    11581 aggcgtcagc atcggggagc gttcgcgcgg tctcaagaag accgtgacca taagcgttcg
    11641 ctttcaccac cgcaaccatt ttactggcag gggccagttc acgaagacgt tgcaggttgt
    11701 gtcgcagagc gcggcggtta atcacaacag ttgccgcttg catttgtgtt ccttgataag
    11761 tgtttgcttt aattacctaa ttcataaaat aattattatt cgtcgtcgta ctgcggcccc
    11821 gcatagttgt cgaagcgcga ccattgaccg ttaaaggtca ggcgtaccgt cccgattggg
    11881 ccgttacgtt gtttaccgat aataatttcc gcgatgcctt ttaaatcact gttttcgtga
    11941 tacacctcat cacgatagat aaacataatc aagtccgcat cctgctcgat agagccagat
    12001 tcacgcaggt cggagttgac cgggcgtttg tcggcacgtt gttccagaga acggttcaac
    12061 tgggacagcg ccaccaccgg cacgttcagt tctttcgcca gtgctttcag cgagcgagag
    12121 atttctgcaa tttccagcgt acggttatcg gaaagcgccg gtacgcgcat cagttgcagg
    12181 tagtcgatca tgataagccc gatgccgccg tgttcacggg caatacggcg tgcgcgggaa
    12241 cgcacttccg ttggcgtcag gccggaggag tcatcgatat agatattgcg tttttcgagc
    12301 aaaataccca tggtgccgga aatgcgcgcc cagtcttcat catcgagctg cccggtacgg
    12361 attttagtct ggtcaacgcg cgacagcgac gccagagaac gcatcataat ctgttctgaa
    12421 ggcatctcca gcgagaagat aagtactggt ttatcctgca acatcgccgc gttttcgacg
    12481 aggttcatcg caaatgttgt tttacccatc gacggacgcg cggcgacgat gatcaaatcc
    12541 gacggctgca agccagcggt ttttttgttg agatcgtcat aaccggtgtt tactccggta
    12601 acgccatcgt gtggctgctg aaacaactgc tcaatacgcg ccacggttgc gtcgagcaca
    12661 tcggcgatgt tcttcggccc ttcgtctttg tttgcacgac tttcggcaat tttaaagacg
    12721 cgggattcag caaggtccag cagatcttcg ctggtacgcc cctgcggatc aaaaccggct
    12781 tcagcaatct cattcgcaac cgagatcatc tcacggacaa cggcacgttc acgcacgatg
    12841 tcagcataag cactgatgtt cgccgcactt ggcgtatttt ttgacagctc tgccagataa
    12901 gcaaaaccac cgacgctatc gagttgcccc tggcgttcca gcgattccgc aagggtaatc
    12961 agatcgatag gactaccgct ttcctgcaaa cgcgccattt cagtaaagat atggcggtgt
    13021 gggcgggtgt aaaaatcgtc tgccaccaca cgctcggcta catcgtccca gcgttcgtta
    13081 tccagcatta aaccgcccaa caccgactgc tccgcttcga tcgagtgcgg aggcactttc
    13141 agcccggcaa cttgtggatc gcgttcgcgg ggttcagcct gctgtttgtt gaagggttta
    13201 tttcctgcca tagtgaatgg agttaccgag ataaagaatg ggtcgaaact ttaccatatg
    13261 aagcagaccc tgacgatacg ttctggagga cacatggcaa cacgaattga atttcacaag
    13321 cacggtggcc cggaagtact tcaagccgta gagttcactc ctgccgatcc ggcagagaat
    13381 gaaatccagg tcgaaaataa agccatcggc atcaatttta tcgacacgta tatccgcagc
    13441 ggcctttacc cgccgccatc actacccagc ggattaggta ccgaagcggc aggcatcgtg
    13501 agtaaagtcg gcagtggtgt aaagcatatt aaggcaggcg atcgtgtagt ctatgcgcag
    13561 tcggcgttag gcgcttacag ctctgtgcat aacattaatg cggataaagc ggcgattctg
    13621 cctgcggcaa tttcttttga gcaagctgcg gcatccttcc tgaaaggctt aacggtttat
    13681 tatctgctgc gcaaaaccta tgaaattaaa cccgatgaac agttcctgtt ccacgcagcg
    13741 gctggcggcg ttggcttaat tgcctgccag tgggcaaaag ccctgggcgc gaaacttatc
    13801 ggcaccgtag gaaccgcgca aaaagcgcag agcgcgctaa aagcgggcgc gtggcaggtt
    13861 attaactatc gtgaagagga tctggtcgag cggttaaaag agatcaccgg cggtaagaaa
    13921 gtgcgcgtgg tgtacgattc cgtgggcaga gacacctggg aacggtcgct ggattgcctg
    13981 caacgccgcg gcttaatggt cagttttggc aactcatcag gtgcggttac cggtgtgaac
    14041 ttaggcattc tcaatcaaaa aggctcgttg tatgtgacac gcccttccct gcaaggctat
    14101 atcaccacgc gggaggaatt aaccgaggcc agtaatgaac tgttctcttt gattgccagc
    14161 ggtgtgatta aggtcgatgt cgccgagcag cagaaatatc cgctgaagga tgcgcagcgt
    14221 gcgcatgaga ttctggaaag ccgggcgacg caaggttcca gcctgttgat tccataaaag
    14281 aaatagggct tccacctggg aagccctttc tttttatagt tcggctgtat gtagggtaca
    14341 gcacgatgaa tctgttagag gcgcaatagt gacagatttg attatcaatt cctattttgt
    14401 tctaaggata aaaccttagg ttgtgatcat ccgcacaatc ccttagtaac gccagcggtc
    14461 ataacgctga tatttcggca cttttggtgc tttaatcgcc ttaataaccc acaccaccgc
    14521 aatcgccagt agtaaccacg gcagcaactt aatcatcaat gccagcatac cgccgaggaa
    14581 cataatggcc gtcgccacaa ccagcgcggc gataatgccc agcaacgaaa cgccggtgac
    14641 catcagcatg acaaaaaagc caatcacaaa aagtagttcc agcatgatgc tctcccaaat
    14701 atgaaatctc ttgctggcat tacaagaatc atgccaaaaa taatctattg atttaacagc
    14761 aaaacgcccc gcgacggtgc gcagggcgtg gtgaatttga ctactttttg gtgaaaagtt
    14821 aacgcttatc cgccaccagt ttgagcgcgt gttccagcac attaatgtct gcacccgctt
    14881 tatgggcatt ttcacttaaa taacgccgcc actgccgcgc gccaggaata ccctggaaca
    14941 agcccaacat atgccgggta atatggccga gatacgtccc ctggctgagt tcacgctcaa
    15001 tgtacggata catggcgcgc actaccgcca ccggatcggc atcggtatcc gaggaaccaa
    15061 agatctcccg gtctaccgcc gccagaatac ccggattctg atacgcctcg cgcccgacca
    15121 tcacgccatc catatgttgc aggtgtgctt tggcctcttc cagcgacttg ataccaccgt
    15181 taatcgacat tgtcagatgc ggaaagtcac gcttcagttg atacacacgc ggataatcga
    15241 gcggcgggat ttcacggttt tctttcgggc ttaaccccga aagccaggct ttacgtgcgt
    15301 ggatgataaa catctcacac tcgcctttgc cggaaacggt gttgatgaaa tcgcagagaa
    15361 attcatagct gtcctggtca tcgatgccaa tacgcgtttt caccgtcact ggaatcgaca
    15421 ccacatcgcg catcgctttc acgcagtcgg caaccagctg cgcattaccc atcagacacg
    15481 caccaaacat gccgttctgc acccggtcag acgggcagcc gacattcagg ttgatctcat
    15541 catatccacg cgcttctgcc agcttcgcac actgtgccag cgccgccgga tcgctaccgc
    15601 cgagttgcaa cgctaccgga tgttcttctt cactgtacgc caggtaatca cctttaccgt
    15661 gaataatcgc ccctgtggtc accatttcgg tatacagcaa cgtattgcgg gaaagcagac
    15721 gcaagaaata gcggcaatgt ctgtccgtcc agtcgagcat tggtgcaacg ctaaaccgac
    15781 cactccagtg aacgtcagtt ttttcaggca ttgcgctggt ttggttgatt ttttgcattt
    15841 cagaattacc gtgcattttc aaatgtagag attattttat cgatatatca tggggttatg
    15901 ttattcagca tcactattca ggaggctcaa tagcggggta ctataccata acaacaggaa
    15961 gcgcctgtct cattgcaaaa gaaaattgag atcctctcaa ggcatgaagc tctcacgaag
    16021 tgatggaaat aatcttatta gccgttaact ttgttaaggc caatgataaa caatccaggt
    16081 tcgacgataa ataaaaaatc acacattaaa ctctggtgat atatctccct gctaatagca
    16141 ttgatagaga aaaaagaacc caataaatat tgggtccttt tatataatgc cttccatact
    16201 atcgaagaac ttcacatatt atttctccga tttaaccccg aacaaatcat aaattaattt
    16261 agaagtgtct gtaagtattt taatctcttc ctttgaggtt gggtcaaacg acttcgcgaa
    16321 gctaattaat tgtggtgcag catctcgcat tttgcttaaa atatcaggat cgagcgttcc
    16381 ttcattcacc agatgtgaca tcttatccag atagccatca aaattcattt ctcccccatc
    16441 agccagacgc ttcatctctc ccaggtactt cttcatatca cgttgagata atttttcaaa
    16501 ctgagctttc aggtagctct cattattttc attaatatac actgtttctg ataaatctgc
    16561 gtaggcacta gaatatgaaa gtgaaattaa cagtgaagca aataatttta agctgttgtc
    16621 atcatgttcc tggcaggcat taacaaacgt taaaaaacca gaacctattg cttgaaaatg
    16681 tatattcgct aaaggttcat tatccttaga ttcctcataa aatagctgaa ccgtggaggg
    16741 aaggccattc ttagtttcta catcaaaagt acattgttca atgggagtcg catctttatt
    16801 atccccagga ttacatatac tattaatcgc gaaatgaaaa atagcccggt caacaactga
    16861 tgcaataaag tcacgatcgt ttaaatcctc atcagtgcaa tcgtcaatat acttattgac
    16921 ccacattttg agatcattta tttgctcaat taaaaacaca tttttttacg tgcatgccat
    16981 aaattatgcg tgattcgttg aataaacaaa tctatttttg caataattat atcccgatga
    17041 cgcagcgctg cataatcaat agatttacca tatatattat ttaatttacc tttgattgaa
    17101 cctctattat ctaatttatc tttgattgca tcttcattat cgaattgatt tgaattgagt
    17161 gttttattaa agtatccagt tgcaccgggt attatcttca acatgattca tctacctttt
    17221 ttgtcgtacc gtgtcctcct tataattctt ttgccatctg gtcactatca aaaatcgctc
    17281 ataatttctg aactacaggt atgcgtttaa acccagttaa ataaatagcg agttaacttg
    17341 aagcaattta tttaaaaatg aattattaat tattatcgtg ttgataatgg tacgacaagg
    17401 caacataaca ccttacctac aaaacagaaa atcaacatta cggcaacaat aagggttctc
    17461 ggtgtttttc ctggaacatg gtaaagtaag gacattctta acccccactt tgaggtgccc
    17521 gatggaaaag accacaacgc aggagttatt agcgcaggct gaaaaaatct gcgcgcagcg
    17581 taatgtgcgc ctgaccccac agcgcctgga agtgttgcgc ctgatgagtc tgcaggatgg
    17641 cgctatcagc gcttatgatc tgcttgattt actgcgcgaa gctgaaccgc aagccaagcc
    17701 gccaacggtt tatcgcgcgc tggattttct gcttgagcaa ggttttgtgc ataaggtgga
    17761 atccaccaac agttatgtgc tctgtcatct gttcgaccag cccacgcata cgtcagccat
    17821 gtttatatgc gatcgctgcg gcgcggtgaa agaagagtgt gcagaaggcg tggaagacat
    17881 tatgcatacg ctggcggcaa aaatggggtt tgccctgcgg cataatgtga ttgaagcaca
    17941 tgggctctgt gcggcatgcg tggaagtgga agcgtgtcgt catcctgaac agtgccagca
    18001 tgatcactct gtgcaggtga aaaagaaacc gcgttaagag ggcgtacatc cttgtacacg
    18061 tcgggcagga gggattaatt accagcgata ttcattgcgg gtttcccaat ccacgacctc
    18121 tttttctgcc tgatctttct gataaccata acgttcctgg attttaccga ccagttgatc
    18181 acgtttacct tcaatgatcg tcatatcatc atcggtcagt ttgccccatt gctctttcac
    18241 tttaccttta aactgtttcc agttaccgcc ggcttcatct ttattcataa tcaagacctc
    18301 atcgttaggt tgtgaatgag agtacgttca cttttcttct gaacgtgaga ttaagtgtag
    18361 tcagcaattt ggcttaggat aatttattca gaatttttaa ccgtcacgtt gcggcaaacc
    18421 aggtaccgtt acgccagtga cgccgccaga tagccgccag agaaagcccg cgcaacgcca
    18481 gaaagacggt tagtgccagc cacaaaccat gattacccag ccacggcagc gtaaggagcg
    18541 tcagcgcaaa acctgcggcg gccaccgcca tactgttacg catttcggcg gcacgcgttg
    18601 cgcctataaa catgccgtcc agcagataac accagacgcc aaccaacggc aaaatcacct
    18661 gccagataag atagcggtca gccagctgct gaatctgggt taacgacgtc agcaacgcaa
    18721 tgatgtgttc ccctgccagc aaataaacca ccgaaaatag taacgctacg atccccgact
    18781 ggcggcacgc tgcccgccag acatccagca actggctacc gtcgcgcgca ccgtatgcct
    18841 gaccggaatg tgcttcaacc gcgtaggcaa aaccatccag cgcataggcg gtaaaggtga
    18901 gtagcgtcat cagaaccgcg tttacagcga taatgtcact ccccagtcgc gcgccaagta
    18961 cggtgatcgc gccgaaacag agttgcaaca acagcgaacg cagcatgata tcgcggttaa
    19021 gcgccagcaa gcgacggaag tttcctcgcc aggcagtttt cgacatttcg ccggaaattc
    19081 cgcgtagttt gaggatttta cgcaccatta gcagaccaat cagcaatgtt gcatattccg
    19141 caataaccgt cgccagagcc gcgccctgca cgttcatatg cagccccatc actagccaga
    19201 catccagcac aatgttgagg atattgccga ccactaacaa aattactggc gcacgggcat
    19261 attgcacgcc gagtaaccaa ccgagtaata ccagattcgc cagcgacgcc ggtgcgctta
    19321 accagcggat ttcaagaaag cgccgcgcct gttccaggac tgcttcacta ccgccaacaa
    19381 tatgcagagc cagatcgata atcggtgtac gcagcagcgc aattaacgcc ccagccccca
    19441 acgccaacag caacggttgc accagcgcac gggctaatgc ctgaggattt ttggcaccat
    19501 aagcctgcgc agtcagcccg gtggtgctca tgcgtaaaaa cagcaacagc ataaagagaa
    19561 agctggtcgc cgttgcacca accgccacgc cgcccaaata aaccgggcta tcaagatgac
    19621 caattaccgc cgtatcgacc agtcccagca acggaacggt gatattggag aaaatcatgg
    19681 gtaaggcgag atgccagagt gctttatcag atgaagtgag gaatgccatg cagacaagcc
    19741 tgatgaagag agatgaaaaa caaaccgcga cgcctggcgg catcgcggtc tcagagatat
    19801 gttacagcca gtcgccgttg cgaataaccc caaccgccag cccttcaatg gtgaagctct
    19861 gctgacgaag gtcaacgaca attggtttaa actcgctatt ttctggcaac agttcgactt
    19921 tattaccctg ttttttcagg cgcttaacgg taacttcgtc atcaatacgt gcgacaacga
    19981 cctgaccgtt acgtacatcc tgagttttat gcactgccag caagtcacca tccataatgc
    20041 cgatatcttt catcgacatc ccgctgacgc gcagcaggaa atcagcattc ggcttgaaca
    20101 aggaaggatc gacctgataa tgaccttcaa tatgctgctg cgccagaagc ggttcaccgg
    20161 cagccacacg acctaccagc ggcaaccctt cttcctcttc ctgcaacaga cgaatcccgc
    20221 gtgatgcgcc ggaaacaatt tcaataacgc ctttgcgtgc cagcgccttc agatgttctt
    20281 cagccgcgtt tggggaacgg aaccccaaac gctgcgcgat ttccgcacgc gtcggcggca
    20341 tacctgtctg gctgatgtga tcacggatga gatcaaacac ctcttgttgc ctggccgtta
    20401 acgctttcat tccgccccct gggtgtatat acagttatgc tgtgagtata tacagcaaaa
    20461 ggcgattttg gaaccataaa ctgcacaata aaccagagat ttatcgaaat ctggaagggt
    20521 tatccaaaat gcgaccataa caggatgcac caggttatca cggcgacgat aatcgcaatc
    20581 agcaccgcgg cggatcccat atctttagcg cgtccggaaa gctcatggta ttcagagcca
    20641 attcggtcaa ccactgcttc gatggcgcta ttgagaattt ccacaatcat caccagcatc
    20701 acggagctga taagcagcac gcgggtaatt gcgtccacat ccagccagca ggcgatgacc
    20761 accgccaaca ataccgctac gccttcctga cggaatgccg cttcgttgat ccatgcagcg
    20821 cgtaaacctt tccaggaata gccagcagct ttgataattc gggtgaatcc agtggtatta
    20881 ttggccattg aaagaacctt tttacattat gagcgtcaat atcagtgtac cgttaaatcc
    20941 gatgattcgg gtttgcgtaa cgccgcgcga aacatgagcg gataccacag aatttcccat
    21001 gactttctgc tatccttgcc gcgcatttgc attattaacc agaggcttta catcgtttat
    21061 gtccggctgg ccacgaattt actacaaatt actgaattta ccattaagca tcctggtaaa
    21121 aagcaagtct attccggcag atcctgcccc ggaactgggg ctggatacct ctcgtccaat
    21181 tatgtacgtt ttaccgtaca actcgaaagc agatttgctg acgttacgcg cccagtgtct
    21241 ggctcatgac ttgcctgacc cgttagagcc gctagaaatc gacggcacgc tactgccgcg
    21301 ctatgtgttc attcacggcg ggccgcgtgt attcacctat tacacgccga aagaagagtc
    21361 tattaagctg ttccacgact atctcgattt gcaccgtagc aacccaaatc tggatgtgca
    21421 gatggtgcca gtatcggtga tgtttggtcg cgcgccgggg cgtgaaaaag gcgaagtaaa
    21481 cccgccgctg cgtatgctta acggcgtaca gaaatttttc gccgtactgt ggctcggtcg
    21541 cgacagtttt gtgcgtttct cgccgtcagt ttcgctgcgc cgtatggcgg atgaacacgg
    21601 cacggataaa actatcgcac agaaactggc gcgcgtggcg cgtatgcact ttgcccgtca
    21661 acgtctggct gccgtaggcc cacgtctgcc tgctcgtcag gatctgttta ataagctgct
    21721 cgcctcccgc gccattgcca aagcggtaga agatgaagcg cgcagcaaaa aaatctccca
    21781 tgaaaaagcg cagcagaacg cgattgcgct gatggaagag attgcggcga atttctctta
    21841 cgagatgatc cgcctgactg accgcattct gggcttcacc tggaaccgac tttatcaggg
    21901 catcaacgtc cataacgccg agcgcgttcg ccagctggcc cacgacggtc atgagctggt
    21961 gtatgtgcct tgccaccgca gtcacatgga ctacctgcta ctttcttacg tgctgtacca
    22021 ccaggggctg gtgccgccgc atatcgccgc cgggatcaac ctgaacttct ggccggccgg
    22081 gccgattttc cgccgtctgg gggcgttctt tattcgccgt acttttaaag gcaataaact
    22141 ttattccacc gttttccgtg agtatctcgg cgaactgttc agccgtggtt attccgtcga
    22201 gtacttcgtg gaaggcggtc gttcccgcac ggggcgtttg ctggatccga aaaccggtac
    22261 gctgtcgatg accattcagg cgatgctgcg tggcggcacg cgtccgatta cgctgattcc
    22321 gatctatatc ggttatgagc acgtcatgga agtgggtact tacgccaaag aactgcgcgg
    22381 cgcgacgaaa gagaaagaga gcctgccgca gatgctgcgc ggtttaagca agctgcgtaa
    22441 tctcggtcag ggttacgtca acttcggtga accaatgccg ttgatgacct accttaacca
    22501 gcacgtacca gactggcgtg aatctatcga tcccatcgaa gcggtgcgtc cggcctggtt
    22561 aacgccgacg gtcaataata ttgctgccga tctgatggta cgcattaaca acgcaggcgc
    22621 ggcaaacgcc atgaacctgt gttgtactgc gcttctggca tcacgtcagc gctcactcac
    22681 ccgcgagcag ttaaccgagc aactcaactg ctacctggat ctgatgcgca acgtacctta
    22741 ctccacggac tctaccgttc cttcagccag cgccagcgag cttatcgatc acgcgctgca
    22801 aatgaacaag tttgaagtcg agaaagacac tatcggcgac atcatcattc tgccgcgcga
    22861 gcaagcggtg ctgatgacct actatcgcaa caacattgcg catatgttgg tgctgccttc
    22921 gctgatggcg gcaattgtca cccagcatcg ccacatctcc cgcgacgtat tgatggagca
    22981 cgtcaatgtg ctttacccaa tgctgaaagc ggagctgttc ctgcgctggg atcgcgacga
    23041 gttgccggac gttattgatg cgctggcaaa tgagatgcaa cgtcaggggc tgattaccct
    23101 gcaagatgat gagttgcata tcaacccggc gcattctcgc cccctacagc tgctggccgc
    23161 aggcgcgcgc gaaacgctgc aacgttatgc catcaccttc tggttgttga gtgccaaccc
    23221 gtcgatcaac cgcggtacgc tggagaaaga gagccgcacc gttgcgcaac gtctctccgt
    23281 gctgcacggc atcaacgcgc cggagttctt cgacaaggcg gtgttcagtt ctctggtgct
    23341 gacgctgcgt gatgaagggt atatcagcga tagcggcgac gccgaaccgg cagaaacgat
    23401 gaaggtttat cagttgctgg cggagttgat tacatcagac gtgcgtttga cgattgagag
    23461 tgcgacgcag ggcgaagggt aatcagagag aattgccgga tgcggcgaaa acgccttatc
    23521 cggccttcca taatctgcaa attcaataaa ttgcgatgtg ttgtgtaggc ctgagaagcg
    23581 cagcgcatca ggcaacccag aagaaagccg gatgatcatc cggctttttt acatcatcag
    23641 aaatgccagt aactcattgc cagtcctaaa aacagtacca gaccaacata gttattattc
    23701 ataaatgctt taaagcaggc ttcacgctcg cggttggcaa tcagtttttg ttgataaaca
    23761 aacagcgcgc cagccaccag aattgaccag taatatcccc agcctaagcc atttaactca
    23821 ccgatgatcg ccatcagtgc cagtacgcca atctgcaaaa taccaataat caatttatcg
    23881 tattggccga acaggattgc ggtggattta ataccaatct tcacatcatc gtcacggtca
    23941 accattgcat actgcgtgtc gtaagccacc gcccagagaa tattggcgag gaacattaac
    24001 cagcaactca atggcaccga ctcactcaca gcggcaaaag ccattggaat cgaccagcca
    24061 aacgccgcgc ccagcaccac ttgcgggaga tgggtatacc gcttcataaa cgggtacacc
    24121 cacgccaacg ccagagcggc aatcgacaac agaatggtca tcgtattcag cgtcagcacc
    24181 agtaaaaatg aaatcaatac cagcactaca aacagcgcgc gcgcctcttt ctccgttacc
    24241 gcgccgctgg gaagcggtcg gttcgccgtg cgcttaacat gaccatcaaa cttgcggtca
    24301 gcataatcat tcaccacaca tccggcagcg cgcatcagcc agacacccgc gacaaacacc
    24361 gccaggatcc agagctgggg aacgcccggt gtcgccaccc acaacgccca taatgttggc
    24421 cagagcagca gtaacgcgcc aattggctta tccgtacgca ttaagcgatg aaacgccagc
    24481 agcttattct gcgtcagact ccactccata ttttttcctc ttagtacaac ggtgacgccg
    24541 gtaaaaacag ttctgttagc aacagcggtt taccgcttaa tcgcaggcgg gaacgtcgcc
    24601 cccacagtcc ggcatcacgg cctatctcaa taaagtcccg ggttaatgtc gatgatgtga
    24661 acagatagcg tcctagcggc gttttaccca atttttgtaa cgccagctcc ggcccgctta
    24721 acgttgacac aggaacgacg gtacgaccgg caagccacgg ttcaccatcg gcacataaca
    24781 aaatttcacg taaccagtaa cgagactctt tcggcagcag cggcagttct tcggggattt
    24841 cattctgctc gacaaaccct tcgcggatca tcgtcacgct caccgttttt ccctgctgtt
    24901 caaaacgttt tgtcatggaa tcttccagca acagccagtc gagcagttgc gaatccaggg
    24961 cagggatctc tttaaaatag cgcagcgcac gcagttgcgt taacgcgggg tgtgacatgc
    25021 cgaactctcc gttacataac gtaaaggcat tgtatcgcag aaaagtgttt acgggagatg
    25081 taaagcaaat taagtgatct aatgcgcaac aaaaacgcaa caatcgcatg aagtggtaaa
    25141 acgctgttga accttacgct ttgccgatgc ccgtgctggc gagggcaacc gtaggggcat
    25201 caccgcccat gatgccagga acggtgatgt tttcctttac ttaaatacct atattcttta
    25261 tacgagtagc tcgccaactc gcagtaaaaa ttgggaaaag cgcatattta ccatgagatt
    25321 tggagctaaa aagtgtttcc tgagtaagat taataggcaa catcttatgt ctggatttat
    25381 agatacacgt ggaattccaa ttggtatctt ttgtaggatt caacatat
//
LOCUS       DAJTVJ010000056        25103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000056 DAJTVJ010000000
VERSION     DAJTVJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.056"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            194..382
                     /locus_tag="OO448_002215"
     CDS             194..382
                     /locus_tag="OO448_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6337414.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="OO448_002216"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="OO448_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6337415.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="OO448_002217"
     CDS             1143..1307
                     /locus_tag="OO448_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6337416.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="OO448_002218"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="OO448_002218"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR6337417.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6110
                     /gene="bcsB"
                     /locus_tag="OO448_002219"
                     /pseudo
     CDS             3772..6110
                     /gene="bcsB"
                     /locus_tag="OO448_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            6117..7223
                     /gene="bcsZ"
                     /locus_tag="OO448_002220"
     CDS             6117..7223
                     /gene="bcsZ"
                     /locus_tag="OO448_002220"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6337418.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7205..10697
                     /gene="bcsC"
                     /locus_tag="OO448_002221"
                     /pseudo
     CDS             7205..10697
                     /gene="bcsC"
                     /locus_tag="OO448_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10779..12767
                     /gene="hmsP"
                     /locus_tag="OO448_002222"
     CDS             10779..12767
                     /gene="hmsP"
                     /locus_tag="OO448_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6337419.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12950..14236
                     /gene="dctA"
                     /locus_tag="OO448_002223"
     CDS             12950..14236
                     /gene="dctA"
                     /locus_tag="OO448_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6337420.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14456..15952
                     /locus_tag="OO448_002224"
     CDS             14456..15952
                     /locus_tag="OO448_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6337421.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16048..16977)
                     /gene="kdgK"
                     /locus_tag="OO448_002225"
     CDS             complement(16048..16977)
                     /gene="kdgK"
                     /locus_tag="OO448_002225"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6337422.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17209..17976
                     /gene="pdeH"
                     /locus_tag="OO448_002226"
     CDS             17209..17976
                     /gene="pdeH"
                     /locus_tag="OO448_002226"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6337423.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18046..20106
                     /locus_tag="OO448_002227"
     CDS             18046..20106
                     /locus_tag="OO448_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6337424.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20288..21610)
                     /locus_tag="OO448_002228"
     CDS             complement(20288..21610)
                     /locus_tag="OO448_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6337425.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22021..23034)
                     /gene="yhjD"
                     /locus_tag="OO448_002229"
     CDS             complement(22021..23034)
                     /gene="yhjD"
                     /locus_tag="OO448_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6337426.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23083..23982)
                     /locus_tag="OO448_002230"
     CDS             complement(23083..23982)
                     /locus_tag="OO448_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6337427.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24502..>25103
                     /locus_tag="OO448_002231"
     CDS             24502..>25103
                     /locus_tag="OO448_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6337428.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5853 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cggctgccag
     4201 tgaccaaaga acagttgggt aaaaaaacgc tggcgcaaat gcccattaac ccactgttta
     4261 ttaccgactt caaccgtgtg cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa
     4321 acccggccag caccacgctt tggctggatg ttgggcggag cagtggactg gatctgacct
     4381 atcagaccct gaatgtgaag agtgacctgt cacacttccc ggtgccattc tttgacccgc
     4441 gcgataaccg tactaacacc ttgccgatgg tctttgcggg tgcgccggat gttgggctgc
     4501 aacaagcatc tgccattgtc gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga
     4561 acttcccggt gctctataac caattgccgg atcgcaatgc cattgtcttt gcaaccaacg
     4621 acaaacggcc ggacttcctg cgcgatcatc cggcggtaaa agccccggtg attgagatga
     4681 ttaaccatcc gcagaatcct tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag
     4741 acctgttgca ggcagcgaaa ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg
     4801 tggtagtgaa tgaagtgaaa ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg
     4861 tacgtaccga tcgtccggtc acatttggcg aactgaaaac ctatgaagaa cagttacaat
     4921 ccagcggtct tgagccagca gcgattaacg tttcgctaaa cctgccgccg gatctctacc
     4981 tgatgcgcag taccggcatt gatatggata ttaattaccg ctacaccatg ccgccggtga
     5041 aagacagttc gcggatggat atcagcctga ataaccagtt cctgcaatcc ttcaacctga
     5101 gcagtaaaca ggaggcgaac cgcctgctgc tgcggattcc ggtattacaa ggtttgctgg
     5161 atggcaaaac ggatgtctct attccggcgc tgaaactggg cgcgaccaac cagctgcgct
     5221 tcgactttga gtatatgaac ccgatgccgg gcggttcggt ggataactgt attaccttcc
     5281 agccggtgca gaatcatgtg gtgattggtg acgattccac catcgacttc tcgaagtatt
     5341 accacttcat cccgatgccg gatctacgcg cctttgctaa cgcgggcttc ccgttcagcc
     5401 ggatggcgga tctgtcgcaa accatcaccg tgatgccgaa aacgcctaac gaagcacaga
     5461 tggaaacgtt gctgaatact gttggtttta tcggcgcaca gacgggcttc ccggcgatta
     5521 atctgacggt gaccgatgat ggcagcacca ttcagggcaa agatgccgac atcatgatca
     5581 tcggtggtat cccggacaaa ttaaaagacg ataagcagat cgacctgttg gtgcaggcga
     5641 ccgaaagctg ggtgaaaaca ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg
     5701 agagcgatcg tgcggcagaa acccagtcaa cgctgacctc ttccggtgcg atggcggcgg
     5761 tgattggctt ccagtcgccg tataacgacc agcgcagcgt gattgcgctg ctggcagata
     5821 gcccacgcgg ttatgaaatg cttaacgatg cggtgaacga tagcggcaaa cgcgccacca
     5881 tgttcggttc ggtcgcggtg atccgcgagt ccggtatcaa cagcctacgt gttggcgacg
     5941 tttattacgt aggtcatctg ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc
     6001 cgattctgct ggcggtgctg gcggcaatca gtgtgatatt gctggcatgg gtactgtggc
     6061 gtctgctgcg aattattagt cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga
     6121 atgtgttgcg tagtggaatc gtgacgatgc tgctgttggc tgcctttagt gttcaggcag
     6181 cctgtacctg gcctgcctgg gagcagttta aaaaggatta catcagtcag gaagggcgcg
     6241 tcatcgcccc cagcgacgcg cgcaaaatca ccacctccga agggcaaagt tacggtatgt
     6301 tctttgccct ggcggctaac gaccgtgtag ctttcgataa tattctcgac tggacgcaga
     6361 acaatctcgc tcagggctct ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag
     6421 agaacagtaa gtgggaagtg ctggacagca attcggcctc cgatggtgat gtctggatgg
     6481 cctggtcgtt gctggaggcg gggcgtttgt ggaaagagca gcgttatacc gacatcggca
     6541 gcgcgttgct aaaacgtatc gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca
     6601 tgttgttacc gggcaaagtg ggttttgctg aggataacag ctggcgtttt aaccccagct
     6661 acctgccgcc gacgctggcg cagtatttca cccgctttgg cgcgccgtgg accacgctgc
     6721 gcgaaaccaa tcaacgttta ttgctggaaa ccgccccgaa aggtttttcg ccagactggg
     6781 tgcgctatga gaaagacaaa ggctggcagc taaaagccga aaaaacattg atcagcagct
     6841 acgacgctat ccgcgtttac atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag
     6901 cgcggatgct caaccggttt aaaccgatgg cgacattcac tgagaaaaac ggttatccgc
     6961 cggaaaaagt ggatgtggct acggggaaag cgcagggtaa aggaccgatc ggtttttctg
     7021 ccgccatgct gcccttttta caaaaccgcg atgcgcaggc cgttcagcgc cagcgcgtgg
     7081 ccgataactt tcccggcagc gatgcctatt acaactatgt gctgaccctg tttggacaag
     7141 gctgggatca acaccgtttc cgcttctcga caaaaggtga gttattacct gactggggcc
     7201 aggaatgcgc aaattcacac taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc
     7261 gatggtcgag gcagcaccaa ccgctcagca acagttgctg gagcaagttc ggttaggcga
     7321 agcgacccat cgtgaagatc tggtgcaaca gtcgttatat cggctggaac ttattgatcc
     7381 gaataacccg gacgtcgttg ccgcccgttt ccgttctttg ttacgtcagg gcgatattga
     7441 tggcgcgcaa aaacagctcg atcggctgtc gcagttagcg ccgagttcaa atgcgtataa
     7501 atcgtcgcgg actacgatgc tactttccac gccggatggt cgtcaggcac tgcaacaggc
     7561 acgattgcag gcgacgaccg gtcatgcaga agaagctgtg gcgagttaca acaaactatt
     7621 caacggtgcg ccgccggaag gtgacattgc tgtcgagtac tggagtacgg tggcgaaaat
     7681 tccggctcgc cgtggcgaag cgattaatca gttaaaacgc atcaatgcgg atgcaccggg
     7741 caatacgggc ctgcaaaaca atctggcgct attgctgttt agtagcgatc gccgtgacga
     7801 aggttttgcc gtcctggaac agatggcaaa atcgaacgcc gggcgcgaag gggcctctaa
     7861 aatctggtac gggcagatta aagacatgcc cgtcagtgat gccagtgtgt cggcgctgaa
     7921 aaaatatctc tcgatcttta gtgatggcga tagcgtggcg gctgcgcaat cgcaactggc
     7981 agaacagcaa aaacagctgg ccgatcctgc tttccgcgct cgtgcgcaag gtttagcggc
     8041 ggtggactct ggtatggcgg gtaaagccat tcccgaacta caacaggcgg tgcgggcgaa
     8101 cccgaaagac agtgaagctc tgggggcgct gggccaggcg tattctcaga aaggcgatcg
     8161 cgccaatgca gtggcgaatc tggaaaaagc cctcgcactg gacccgcaca gcagcaacaa
     8221 cgacaaatgg aacagtctgc tgaaagtaaa ccgctactgg ctggcgatcc agcagggcga
     8281 tgctgcgctg aaagccaata atcctgaccg ggcagaacgc ctgttccagc aggcgcgtaa
     8341 tgtcgataac accgacagtt atgcagtgct ggggctgggc gatgtggcga tggcgcgaaa
     8401 agattatccc gccgccgaac gttattatca gcagaccttg cgtatggaca gcggcaacac
     8461 taacgccgtg cgcgggctgg caaatattta ccgccagcaa tcgccagaaa aagctgaagc
     8521 gtttatcgcc tcgctctctg ccagtcagcg gcgtagcatt gatgatatcg aacgcagcct
     8581 gcaaaacgac cgtctggcac agcaggcaga ggcactggaa aaccagggca aatgggcgca
     8641 ggcggcagca cttcagcggc aacgactggc gctggacccc ggcagcgtat ggattactta
     8701 ccgactttcg caggatctct ggcaggccgg acaacgcagc caggccgata cgttaatgcg
     8761 caatctggcg cagcagaagt cgaacgaccc agagcaggtt tacgcttacg ggctgtacct
     8821 acgcttacgg gctgtacctc tctggtcatg accaggacag agcggcgctg gcgcatatca
     8881 acagcctgcc gcgtgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa
     8941 gcgatcaggt gctggaaacc gctaaccgcc tgcgagaaag cggcaaagag gcagaagcgg
     9001 aagcgatgct gcgccagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg
     9061 cgcaacaacg acgtgattac accgccgccc gcgctgcata tcagaatgtc ctgacgcggg
     9121 agccagctaa cgccgacgcc attcttggtc tgacggaagt ggatattgct gccggtgaca
     9181 aagcggcggc acgtagccag ctggcgaaac tgcccgctac cgataacgcc tcgctgaaca
     9241 cacagcggcg cgtggcgctg gcacaggcgc agcttggcga taccgcagca gcgcagcgga
     9301 cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg
     9361 tgctgcgtga tggtgcgaag tttgaagcgc aggcgggcga tccaacgcag gcgctggaaa
     9421 cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca
     9481 cctttacccg actgacccgt aacgacgaga aagatgactg gctgaaacgt ggcgtgcgca
     9541 gcgatgcggc ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact
     9601 ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg
     9661 tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg
     9721 tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc acctgtacat
     9781 tacaggactg tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct
     9841 ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg
     9901 atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg
     9961 cccaccgtcg gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga
    10021 gcaataccgg gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct
    10081 acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta
    10141 aaaatgtcga agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta
    10201 accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag
    10261 atctgagtgg ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt
    10321 ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt
    10381 ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga
    10441 tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg
    10501 gcttcggcta cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg
    10561 gcacggcaat tgatatccag caggcgaaag attacgcacc cagccatttc ctgctctacg
    10621 tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac
    10681 cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat
    10741 actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa
    10801 caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca
    10861 ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa
    10921 gcattgctcg ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg
    10981 aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag
    11041 tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgc ccggtgccga
    11101 taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtt tactcgctcg
    11161 aacgtccggc aaacccgcag ccaattgcct atctggtact acaggcggat tccttccgta
    11221 tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta cttttgtcgc
    11281 ttatcctgac cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca
    11341 atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat
    11401 taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc
    11461 agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt
    11521 cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac
    11581 aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga
    11641 aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt
    11701 cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg
    11761 tacaggaacc gtggcacgca atcaccttag gtcagcaagt gctcaccatc atgagcgagc
    11821 gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct
    11881 acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc
    11941 gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa gccgcccaga
    12001 agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag tttgctattt
    12061 ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc
    12121 gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt
    12181 gctgtgggct gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg
    12241 cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct gcgctgcaac
    12301 tgatgcaccc aaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc
    12361 cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg
    12421 tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca
    12481 tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga
    12541 aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta
    12601 tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac
    12661 aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcacc
    12721 cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg
    12781 attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt
    12841 gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc
    12901 aggtacccca taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc
    12961 tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg
    13021 ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct
    13081 cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat
    13141 ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt
    13201 cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg
    13261 aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc
    13321 gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc
    13381 atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct
    13441 ccaccgtctg ggcagcaaag gccaactgat ttttaacgtc atcgaaagtt tctcgcaggt
    13501 catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt tcggggcaat
    13561 ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat
    13621 ctgtttctat attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc
    13681 gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact
    13741 ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg
    13801 ctgccgcaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg
    13861 cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat
    13921 ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc
    13981 aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt
    14041 gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc
    14101 gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga
    14161 actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg gcaaaacgca
    14221 cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag tgcgggtgtt
    14281 tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac
    14341 ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta
    14401 gactgactga caacgggcag ttttttatta ccaggatagt tgatcagggg ttcacatgca
    14461 gggcacaaaa attcgacttt tagcgggcgg tttgctgatg atggccactg ctggctatgt
    14521 gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt ccaacggttt
    14581 acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa ttcgcctgct
    14641 ggttaatatc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc acgccatccc
    14701 tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt cattgtggca
    14761 gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg acaccacgct
    14821 gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc tctcttatct
    14881 ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg cgctgcaaag
    14941 tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc gctatcgtct
    15001 gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac ccgttgaagc
    15061 ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga cgctactggt
    15121 ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt ttggcgaact
    15181 gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc gtgcggaagc
    15241 ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt gggatacgcc
    15301 gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg acctggcccg
    15361 cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtaacagca aagacatcgg
    15421 tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca acatcgaatc
    15481 accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg cgaaggttcg
    15541 cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga aactggagct
    15601 gcagaaactg tttgccgcct atgcgcgggc tgataccgat attctgatgg gtcagcggat
    15661 gcgttcgctg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga aactgcggca
    15721 ggatttcctt aacagcctga cggtggagat gttaaatcag gatctgcgtc agcagttgtc
    15781 gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta acatgaaagc
    15841 gttgcaggcg gcctgggatc aaatcatggc cccatcgact gctgctgccg ccacctctgt
    15901 cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt aatgcctgat
    15961 ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag gggtaacact
    16021 ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg ggataatcgc
    16081 gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg cgttttccgc
    16141 actgccgccc gtcaaacata ccgccagata accggcgctg aaagagtcgc cagctgcggt
    16201 ggtatcgatc actttttctt tcggcagttt caccgccgga acatccacta acccttcgcc
    16261 agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt tcacgcccgc
    16321 gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg cgtcttcatc
    16381 gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgttggtaca cctgctgtgt
    16441 ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca cttttccgcc
    16501 gttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg ggcttaagat
    16561 cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt cgcaaatcgc
    16621 cgcagactgc tcactctcca gccagaattt ggcggcggct tcgttccgcc agtagtagaa
    16681 cgtacgctcg ccggtgctgt cggtttcaat gtagtaaagg cccggcagac ggttttccat
    16741 ccgttgggtc agggaagtat caacgttctc gccgtgccag gcgtccagca tttgctggct
    16801 aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag gatcgacctg
    16861 acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct taacgtccgc
    16921 gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt tggacatagt
    16981 cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt gctggtcaac
    17041 catattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga aagattccgt
    17101 tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttgc gcgccgataa
    17161 tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat gataaggcag
    17221 gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga acggcgtttt
    17281 tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg cggacggtta
    17341 atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca acgcctgccg
    17401 ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt gaaagagcag
    17461 attgatttgc tggcgcaaaa agccgacttc tttgtagagc acgacctgct ggcatcggtc
    17521 aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct gcgccaaatt
    17581 gagcgtcttc cctggctgcg tttcgaactg gtggaacaca tccgcctgcc gaaagattca
    17641 acctttgcct cgatgtgtga atttggcccg ctgtggctgg atgattttgg caccgggatg
    17701 gcaaatttct ctgcgctaag tgaagtgcgt tatgactaca tcaaaatcgc gcgagagctg
    17761 ttcgtgatgc tgcgtcagtc gccggaaggc cgcacactct tttctcagct tttacatcta
    17821 atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga agagtggcgt
    17881 gatgttcaga actcgcccgc attcgccgca caaggctggt ttctttcacg cccggcaccg
    17941 atggaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc cgctgggctg
    18001 gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag caaggcaggc
    18061 aaaataaccg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc gatcattttg
    18121 attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt ctctgcggag
    18181 ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg gcaaaaacaa
    18241 gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga catcattctt
    18301 ggtaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt agaggcaacg
    18361 ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc cgtggttcaa gctcgaaaag
    18421 cccgacgcgc gcctgattcg cctctccgaa aagaacaata actggacgtt taatcttgcc
    18481 aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat ggtcgtttcg gctggataat
    18541 attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc ggatctggaa
    18601 atttttgttg atccgttagg caagccgctg ccgttcagcg aagttactgg atcgaaaggt
    18661 aaagcggata aagaaaaggt gggcgattac gtttttggcc tgaaggcgca gggacgttat
    18721 aacggtgaac cgctcacggg tacgggaaaa ataggcggta tgctggcgct gcgtggcgaa
    18781 gggacgccgt ttccggtaca ggctgatttc cgttcaggta atacccgtgt tgcttttgat
    18841 ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt tacggcttaa attttctggc
    18901 gattcactgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac cccgccgttt
    18961 gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt ctttgattat
    19021 cgcggtttta atgggcgaat tggcgatagc gatatccacg gttctctggt ctacaccacc
    19081 ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg gctggcggac
    19141 ctgggaccgt tgattggcgt tgattccggg aaaggggcag aaaagtcgaa acggtctgaa
    19201 cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta tgaccgcttc
    19261 gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaagggcg gcgcattgag
    19321 catggcagta gcctgccgat tagcgatctt tctactcata tcatcctcaa aaatgctgac
    19381 ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca gcattgcgac gaatattcat
    19441 ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg tcgactgaaa
    19501 ctgaaagaac tgatgcccga tgtggaactg atgcagaaga cgctggggga aatgaacggt
    19561 gacgcggaac tacgcggtag cggtaactcg gtggcggcgc ttttaggcaa cagtaacggc
    19621 aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga gattgttggg
    19681 ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg acgatgaggt gcgggtgaac
    19741 tgcgcggcgg cgaatctgaa tattgccaac ggcgtggcgc gcccgcagat ttttgctttc
    19801 gatactgaga acgcgttgat taatgttacc ggcacggcaa gttttgcttc agaacagctg
    19861 gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg ttcgccgctg
    19921 tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccggacc gctgattgcc
    19981 cgtggtgctg ttgctgcggc actggcaacg ctggtaacac cgacggcggc gttactggca
    20041 ctgatctcac cttccgaagg ggaggctaat cagtgtcgga cgatattgtc gcagatgaag
    20101 aagtgatgat aattgccgga tgtctgacat ccggcaatta aacagattta gatttttggc
    20161 aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaatcctt
    20221 gtgaattcaa tatattgtat ggaaattcag tcctgataag cgtagcgcat caggcttttc
    20281 acacttatta caacgactga tgtcgcgtct catgggtcag cagcagggcg attaacgtca
    20341 agccagccat cgccgccaga tataacccca ccgcacctaa cccgtagtta gtctgcaacc
    20401 aggctgcgat atacggtgca acggaagccc cgagaatcga cgctacgttg taagagaatg
    20461 atgctccggt gtaacgcact tctgttggga acagctctgg taacagcgcg cccatcggcc
    20521 cgaaggtcag ccccatcaga cttaacccca gcagcaggaa ggcaaaaacc agaatcgggt
    20581 tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa caggatgatc agcgtggtga
    20641 taattaccat gcttttacgg cgaccaaagg catcagccag taatccggcg actggcacca
    20701 tcacgccaaa accaataact gccatcatca gcatccacaa cacttcgtta cgcggcaggc
    20761 caagcccaac tggcgcggcg gcggtactga aggtcataga gtagaccgtc atgatgtaaa
    20821 acagcgtata ggttgccagc atgatgaacg tacccagtac ggttacgcga acatgtttgg
    20881 tcagcagcgt acccagcggg atcttcacct gttttttcgc tttagcgact ttctcaaaca
    20941 ccggcgactc atgcagcgac acgcgaacat acaggccgat aatgaccagt accgccgaga
    21001 agataaatgg cacacgccag ccccagctca taaactgctc gtcggtcagt agccaggaaa
    21061 gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg tgcgcccagc tgtgggaagg
    21121 agccatacag tgcacgtttg cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc
    21181 cccattcacc gcctaagccc agaccctgac caaatcgagc cagcgccagc agcagcgggg
    21241 cgaaaatacc aatcgtggca tagcccggca gcagaccaat caccacggtt gaaatcccca
    21301 tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa
    21361 aaacggcaga gccaatgggg cgcgcgacga aggcgatggc gaaggtggcg agcgactgta
    21421 gcgttgctgc cgttggatcg ccctgcggga agaagatatg cggaaacaca ataacggccg
    21481 cagtggcgta aatgtaaaag tcgaagaact caatggccgt gccaatgaga gaggcgacaa
    21541 ggactttatt acgcgagttg atcggcgtgt attcttgctc gtggtcgagt gttgtggctg
    21601 ttgcttgcat aatcttttct tattttttgg ctaacgaata gccatattac gcacagcaaa
    21661 agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa aaaccaggca aaaaggggat
    21721 aatgtttagg ccagtgattt ttcttctatg aaatgcttca cataatttta taaatagtgg
    21781 ataaaactgg tttcaggtta aataaaagtt accaactgga tttatatgct ggggttatga
    21841 gttggaatga aattgtttgc tcccaaatag atggctgaag aggctctggg gaagagtttc
    21901 aagaggttat gtgcgcatta tagccggatg cgatgctggc gcatcttatc cggcctacgg
    21961 ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt tacgccgcat ccggcctatt
    22021 ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca
    22081 cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa aatgccatca gccccagcac
    22141 ggaaccaaat gctgcgccag agggggattt catcagcgat ggcagggtgt aggtcatcac
    22201 gattttaatc acttcaaaac caatagcagc cagaaatgtt ccgcgaatca gggctttttt
    22261 gcgcggacgg tggcgcggta accgccagaa gatccagaag aaaagcagat agttggcgaa
    22321 gatggaaatc gccaggccaa tcaaccgcca cgtcggtttc agccactcaa tgctgttcag
    22381 gtgcagggcg ctaataatca tttgctgcgc cgaaccggca accgaggtga tcgaaagcgt
    22441 cacaatcagc gcaatcaaca aaccaatcag cgaaataaaa tcacgtagat attttaccca
    22501 gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg cgcgactggg cgcgaatcgc
    22561 ttcacgcagg ttacccatcc agttgatgcc ggaataaagc gccacagcca ggccgacaag
    22621 ccctacagtc gtacgctgct gaacggcggt gttgatggtg tttttcaacg tggcggctag
    22681 cgtcggatcg ctgatgtttt gcagaatttt gtcgaagata tcctgtagca acatcggatg
    22741 ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc aaaatcggga tcatcgacaa
    22801 aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc agccgatcgt taaagcgttc
    22861 tgtcgcgcga atcaggtgcg caataaccgg ttggcgttgg actttttcgg cagtggtggt
    22921 gacggtttcc agcgcctggc taactttgcc gcctttctcg ctattatcca actgcttaat
    22981 cggctcgtgc tccagatcct gggtgggacg tttgatctcg ttttcctgcg tcatccgctc
    23041 ttttgtcctt ctgttgtggt cattcttaaa aagtatagtc ggtcagtcca ggtactcttt
    23101 cattacgcca gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg
    23161 cggataaacc agcgaaaggg agagcggctc ggcacggtag cctggcaaca cttcaataag
    23221 ctgcccggca cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc
    23281 ggcaagaccg gcggcgagat agttttcgct actgtttacc gtcaacatgc cgccagactt
    23341 aaaccactgg acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg
    23401 cggtgtataa cgcaccattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa
    23461 gcgcgtcaga tagtgcggac tggcacagtt gaccatggtc agtttgccga ggggacgggc
    23521 gataacgcca tcttccggta acgcgccagt gcgtatcacg caatcaaaac catcatgaag
    23581 aatatctacc ggacggttat ggctactcag ttccagctca attcccggat gctgatagag
    23641 aaattccgac agacgcggca gtaacaggtt tttcgcgatt cctgacggga tgtcgatgcg
    23701 taatttaccg ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgctgagatt
    23761 actcaacaca tcttttgccc gttgataata ggtcatgcct tctggcgtga gtttgacccg
    23821 tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg
    23881 cgaaacactt ccctttggca aagcaaagaa atccgccgcg cgggaaaaac tttccagctc
    23941 cgcgactttg atgaacaact gcattgcgtg aattttatcc atttctttga ccgattgttg
    24001 ttcacagcga aacagtaacg cgcatatcgc attgtttatt gttttttcta acatctaatg
    24061 ccgggtttca caatgttgtc atgacgaatg tggcgatttc tcagcaggaa aagggcggaa
    24121 tagctttatg agaaaggtga atccccttaa acagagcgta tttttcagtg attcccttta
    24181 aagtgaatag aggacgtatt ggctatttgt gcttattttt gtctcattgc catttgttat
    24241 tttggcttgt agtcgcggaa tttcattttt aattgcatgc gtactaacca ccaatataag
    24301 gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt
    24361 aactatttat atcggtaact attttctacg cgtcatctct gtagaatggc caccgccaaa
    24421 tggcgaattc ttattttttc tcgcgggtaa tacccttcca ctacttaagt ctttttaacg
    24481 tattcaggtg gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt
    24541 catggaatga aaatcagttt acagcagcgt attccaggag tgagtattca gggggccagt
    24601 caggcagacg agttatggca aaagctggaa agttaccctg aagccttagt tatgctcgat
    24661 ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt gcagtttcct
    24721 gaggttaagg tgttaatcac ggcgacggat tgcaataaac ggtggttaca ggaagttatt
    24781 cattttaatg tgctggccat tgtgcctcgt gattcaaccg tcgagacatt tgcgttggcg
    24841 gtaaatagtg cggcgatggg gatgatgttt ctgcctggag actggcggac gacgccggaa
    24901 aaggacatta aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta
    24961 gcggcgggcg aatcaaataa ggagatcggc agagcgctga atatcagtac cggaacggta
    25021 aaagcgcatc tggaatccct gtatcgccgt ctggaagtga aaaatcgcac ccaggcggca
    25081 atgatgttaa atatctcctc ctg
//
LOCUS       DAJTVJ010000057        24837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000057 DAJTVJ010000000
VERSION     DAJTVJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.057"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(29..829)
                     /locus_tag="OO448_002232"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="OO448_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1396..1761
                     /locus_tag="OO448_002233"
     CDS             1396..1761
                     /locus_tag="OO448_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6337429.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1801..2457
                     /locus_tag="OO448_002234"
     CDS             1801..2457
                     /locus_tag="OO448_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR6337430.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2524..3942
                     /gene="dcm"
                     /locus_tag="OO448_002235"
     CDS             2524..3942
                     /gene="dcm"
                     /locus_tag="OO448_002235"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6337431.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3923..4393
                     /gene="vsr"
                     /locus_tag="OO448_002236"
     CDS             3923..4393
                     /gene="vsr"
                     /locus_tag="OO448_002236"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6337432.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OO448_002237"
                     /pseudo
     CDS             complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="OO448_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5476..6393
                     /locus_tag="OO448_002238"
     CDS             5476..6393
                     /locus_tag="OO448_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6337433.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6472..6654
                     /locus_tag="OO448_002239"
     CDS             6472..6654
                     /locus_tag="OO448_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6337434.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6825..8519
                     /gene="yedQ"
                     /locus_tag="OO448_002240"
     CDS             6825..8519
                     /gene="yedQ"
                     /locus_tag="OO448_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6337435.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8516..9331)
                     /locus_tag="OO448_002241"
     CDS             complement(8516..9331)
                     /locus_tag="OO448_002241"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCR6337436.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OO448_002242"
     CDS             complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="OO448_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6337437.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            10019..10207
                     /gene="dsrB"
                     /locus_tag="OO448_002243"
     CDS             10019..10207
                     /gene="dsrB"
                     /locus_tag="OO448_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6337438.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OO448_002244"
     CDS             complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="OO448_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6337439.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OO448_002245"
     CDS             complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="OO448_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6337440.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OO448_002246"
     CDS             complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="OO448_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6337441.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OO448_002247"
     CDS             complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="OO448_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6337442.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OO448_002248"
     CDS             complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="OO448_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6337443.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OO448_002249"
     CDS             complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="OO448_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6337444.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OO448_002250"
     CDS             complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="OO448_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6337445.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OO448_002251"
     CDS             complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="OO448_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6337446.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OO448_002252"
     CDS             complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="OO448_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6337447.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OO448_002253"
     CDS             complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="OO448_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6337448.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OO448_002254"
     CDS             complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="OO448_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6337449.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OO448_002255"
     CDS             complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="OO448_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6337450.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="OO448_002256"
     CDS             complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="OO448_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6337451.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="OO448_002257"
     CDS             complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="OO448_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR6337452.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21825..22139
                     /gene="fliE"
                     /locus_tag="OO448_002258"
     CDS             21825..22139
                     /gene="fliE"
                     /locus_tag="OO448_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6337453.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22473..22805
                     /gene="emrE"
                     /locus_tag="OO448_002259"
     CDS             22473..22805
                     /gene="emrE"
                     /locus_tag="OO448_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6337454.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22974..23525
                     /gene="ybcL"
                     /locus_tag="OO448_002260"
     CDS             22974..23525
                     /gene="ybcL"
                     /locus_tag="OO448_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6337455.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23535..24332
                     /locus_tag="OO448_002261"
     CDS             23535..24332
                     /locus_tag="OO448_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6337456.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6111 a         6137 c         6352 g         6237 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aatgtattta
      961 aaagatttca aaatgtttct tatctatttg atataattca gaattatgaa ttctaaatat
     1021 aaacatccat gaaaattata aatgtatatc aatatgttat gagttgaatg gaaagatagg
     1081 gagtatcaat gattgttgcg ccaaacggca ggcgtgatca cgcgcacatt aatgaaggta
     1141 taaaaaaagc cggcatcatg ccggcccaaa aaatataaac aagacgataa aaggctaata
     1201 acggggttat catgacacag aaatcaaaaa aggtataatt gtattcagtg ctaatatatt
     1261 gtttgggtga attaataaaa ctattaccaa ggatataaat aaatctttat tgctgttttt
     1321 ccagtaatta ttgccgttgc gcaaaattta tctatttgtt caaaaaatga ttgagtcttg
     1381 actggctcat ccaatgtgga aaaatgtgac ttttatcaca taatagtact aagtctgaat
     1441 tttccgggtt atctcaaaat ggaatacggt tcgacaaaga tggaagagag actctctcgc
     1501 agccctggcg gcaaattggc tttgtgggct ttctatacat ggtgtggcta tttcgtctgg
     1561 gcaatggcgc gttacatctg ggtgatgagc cggatccctg atgccccggt gtcgggattt
     1621 gaaagcgatt taggctctac cgcgggaaag tggttagggg cactggtcgg atttttattt
     1681 atggctctgg tcggggcatt gttaggcagc attgcctggt acacccgacc tcgtcccgca
     1741 cgtagcagac gctatgaata aaaactgcta aggtggcgac tctttggcaa cggagcgcgt
     1801 atggacttac aacactggca ggcacagttc gaaaactggt tgaaaaacca tcatcagcac
     1861 caggacgcgg cacagaagct ggcggcagat gacgatgtcg atatgctggt gattttaacc
     1921 gcctgttatt ttcacgatat tgtgagcctg gcgaaaaatc atccgcaaag gcagcgttct
     1981 tcaatcctgg cggcagaaga gacgcgtcgc ctgctccgtg aagagttcga gcaatttccg
     2041 gcggagaaaa tcgaggccgt ttgtcatgcc attgccgctc acagtttcag cgcgcaaatc
     2101 gcccccttaa caacggaagc taaaatcgtc caggatgcgg atcggctgga agccctgggg
     2161 gcgattggcc tggcgcgcgt gtttgccgtt tcaggggcat tgggcgtggc gctgtttgat
     2221 ggtgaagatc cgttcgcaca gcatcgcccg cttgatgata aacgctacgc gctggatcat
     2281 ttccagacta agttgctgaa gctaccgcaa accatgcaaa ccgcaagagg caagcagctg
     2341 gcgcagcaca acgcgcattt tttagtcgag tttatggcga agctcagtgc cgaactggcg
     2401 ggggagaatg aaggtgtcga tcacaaggtg atagatgcgt tttcacccgc gggataagcg
     2461 cgtggcccta aatggctgta attatgttaa cctgtcggcc atctcagatg gccggttaaa
     2521 tctatgcagg aaaatatatc agtaaccgat tcatacagca ccgggaatgc cgcacaggca
     2581 atgctggaga aactgctgca aatttatgat gttaaaacgc tggtggcgca gcttaatggt
     2641 gtgggtgaga atcactggag cgcggcaatt ttaaaacgtg cgctggcgaa tgactcggca
     2701 tggcaccgtt taagtgagaa agagttcgcc catctgcaaa cgttgttacc caaaccatcg
     2761 gcacatcatc cgcattatgc gtttcgcttt atcgatctat ttgccggaat tggcggcatc
     2821 cgtcgcggtt ttgaatcgat tggcggacaa tgcgtgttta ccagcgaatg gaacaaacat
     2881 gcggtacgca cttataaagc caaccattat tgcgatccgg cgacgcatca ttttaatgaa
     2941 gatatccgcg acatcaccct cagccataaa gaaggcgtga gtgatgaggc ggcggcggaa
     3001 catattcgtc aacacattcc tgaacacgat gttttactgg ccggtttccc ttgtcagcca
     3061 ttttcgctgg ctggcgtatc gaaaaagaac tcgctcgggc gggcgcacgg ttttgcctgc
     3121 gatacccagg gcacgctgtt ttttgatgtg gtacgcatta tcgacgcgcg tcgtccggcg
     3181 atgtttgtgc tcgaaaacgt caaaaacctg aaaagtcacg accagggtaa aacgttccgc
     3241 atcatcatgc agacgctgga cgaactgggc tatgacgtgg ctgatgcaga agataacggg
     3301 ccggacgatc cgaaaatcat cgatggtaaa cattttctgc cgcagcaccg tgaacgcatc
     3361 gtgctggtgg gttttcgtcg cgatcttaat ctgaaagccg attttactct gcgtgatatc
     3421 agcgaatgtt tccctgcaca gcgagtgacg ctggcgcagc tgttggaccc gatggtcgag
     3481 gcgaaatata tcctgacgcc ggtgctgtgg aagtacctct atcgatatgc gaaaaaacat
     3541 caggcgcgcg gtaacggctt cggttatgga atggtttatc cgaacaatcc gcaaagcgtc
     3601 acgcgtacgc tgtctgcgcg ttattacaaa gatggcgcgg aaattttaat cgatcgcggc
     3661 tgggatatgg ccacgggtga gaaagatttt gacgatccgc tgaatcagca acatcgtcca
     3721 cgtcggttaa cgcctcggga atgcgcgcgc ttaatgggtt ttgaagcgcc gggagaagcg
     3781 aaattccgca ttccggtttc ggacactcag gcctatcgcc agttcggtaa ctcggtggtc
     3841 gtgccggtct ttgccgcggt ggcaaaactg cttgagccaa aaatcaaaca ggcggtggcg
     3901 ttgcgtcagc aagaggcaca acatggccga cgttcacgat aaggccactc gcagcaaaaa
     3961 tatgcgcgcg attgccacgc gtgatacggc gattgagaag cgcctcgcca gtctgttaac
     4021 cgggcagggc ctggcgtttc gcgttcagga cgccagtctg cccggaagcc cggattttgt
     4081 cgttgatgaa tatcgctgcg tgatatttac ccatggctgc ttctggcatc atcatcactg
     4141 ctatctgttt aaagtgcctg cgactcgaac cgagttctgg ctggagaaga taggtaaaaa
     4201 tgttgaacgc gatcgccgcg atatcagtcg tttgcaggaa ctcggctggc gcgtactgat
     4261 tgtctgggag tgtgcattac gtgggcgcga gaagctgacg gatgaagcgc ttaccgagcg
     4321 tctggaagag tggatctgcg gcgaaggtgc cagcgcgcag atcgacacgc aggggattca
     4381 tttactcgct tgatgcgtcc tgaataactg gcgcaactac gggttttgcc gggaagagat
     4441 attttcccaa cgtgaccagt accaccgcga agacaattac gccgagcgcc agccattcaa
     4501 tcttcgacag cgtctctccg cccagtcccg tacccagcaa gaccgcgacc accgggttaa
     4561 cgtaggcgta gctggtggcg agagccggac tgacattacg gattaaatac atataagcgt
     4621 tgatggcgat aatcgaacca aacagcgcca gatagccgac cgcaaggaag cctgaaaggg
     4681 aagggagcgc cgtcagtttt tcacctgcaa tcatcgacgc gatcattaac accacgcctg
     4741 ccgccagcat ctcaatcgca cccgccatca tccctacagg taaggtaatg cgcgagccat
     4801 aaaccgagcc aaacgcccag ctaatcgagc cgattaaaat cagaatcgcg ccccacggat
     4861 tgccgcttaa atttccaccg ctattgagca tgatgattcc ggcaagccca atggcgatac
     4921 ccacccattc cagtttgcgc gttttaatgc caaacaggcg gctgaagcac cagggtaaag
     4981 aggggcacgg ttgcaaccac aacggcggcg atgccggaag gaacattttg atgctcggca
     5041 accgtcacca tgccattacc gacagccagc aataacaggc caatcagcgc ggcattgagc
     5101 agcggacgta gcggggggag cttgtgtccg cgcagtagca aaaatgccat caataaaata
     5161 ccggctgcca ggaatcgaac gcctgccatc attaacggag gccagctttc cacgccaatc
     5221 cgaatgacaa aataggttga gccccaaatg atatacaacg caaacagcgc gccaaaaagc
     5281 ggtaacaact ggcggaaacg cataatccct cacggtggaa ataaaaaggt ggttcatagt
     5341 aaacgtgaaa atcattctgc tggcgagaga tataattgca cttgattgta aaaaaaatgt
     5401 tgacctgtga agcaagtatc agagctgccg tttttgcttc atacttacac ccttcaacaa
     5461 taaaaatgag agggaatgct tttggccggg agtagtttac tgacgttgct cgatgatatc
     5521 gccacactac tggacgatat ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt
     5581 gtattagggg atgacttatc gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg
     5641 gaacttcccg tggtctgggg cgtggcgaaa ggatcgctga ttaataaagt gattctggtg
     5701 ccgctggcgc tgatcatcag tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt
     5761 ggtggcgcgt ttctctgctt tgaaggagta gagaaagtgc tgcatatgct ggaggcgcgt
     5821 aaacataaag aagatccggc gcagagccag cagcgtctgg agaagctggc ggcgcaggat
     5881 ccgctgaagt ttgaaaagga caaaataaaa ggggcgattc gtaccgattt tatattgtct
     5941 gccgaaatcg tcgccatcac gctggggatt gtggccgaag cgccgttgct taatcaggtg
     6001 ctggtgcttt caggcatcgc gctggtagtg accgtgggcg tctacggtct ggtaggggtt
     6061 atcgttaaga ttgatgacct ggggtattgg ctggcggaaa aatccagcgc gctgatgcag
     6121 gcattaggta aagggttatt gatcattgcg ccctggttga tgaaggcgtt atcgattgtc
     6181 ggcacgctgg cgatgttcct cgtcggcggc ggtattgtgg tacatggtat tgcgccgctg
     6241 catcacgcca ttgaacattt cgccgggcag caaagtgcag tggtggcgat gatattaccg
     6301 actgttttaa atctgattct tggatttatc atcggcggca tcgtggtgct gggagtgaaa
     6361 gccgtagcga aaatgcgcgg tcaggcacat taatactcgt acaggctatt atgcaaaatt
     6421 cgccatcttc aactaaggtg aacaagtttc actcgaaaaa aggaggcgag tatgagcttt
     6481 atggttagtg aggaagttac ggttaaagag ggcggcccgc ggatgattgt cactggatac
     6541 tccagcggta tggttgagtg tcgttggtat gacggttacg gggtcaagcg ggaagctttt
     6601 catgaaaccg agcttgttcc gggggagggg agtcgttctg cggaagaagt ttgaaagggt
     6661 atcattaaca ccaggccgga tcacttccgg cctggtgtgc caatgcctgc aatgaatgat
     6721 agtcgcgtct tatcatgcca ccattataaa attatcgctg ttttgcggta cgctatcgcc
     6781 aggttatgcc agaatcataa aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa
     6841 aaatggaaaa ccagagctgg ttgaaaaaac tcgcacgccg cctggggcct ggtcatgtcg
     6901 ttaatctctg ctttatcgtg gtattgcttt tttccacctt gctgacctgg cgtgaagtgg
     6961 tagtgctgga agatgcctat atctccagcc agcgtaatca tctggaaaac gtagccaacg
     7021 cgctcgataa gcatttgcag tataacgtcg acaaactgat ctttttgcgt aatggcatgc
     7081 gcgaagctct cgtagcgcca ctggatttca cttcactgcg taatgctgta accgagttcg
     7141 aacagcatcg cgacgagcac gcctggcaaa ttgaactcaa ccgacgacgc accctgtcag
     7201 tcaatggcgt atcggatgca ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa
     7261 gcctcgacaa tgaaattacc gctgcactgg aagttggtta cttgctgcga ctggcgcaca
     7321 acacctcgtc gatggttgaa caggcgatgt atgtctcgcg tgccggattt tacgtttcga
     7381 cgcagccgac cttgtttacg cgcaatgtac caacgcgtta ttacggctat gtcacccaac
     7441 cctggtttat cggccattcg caacgagaaa atcgtcaccg cgcggtacgc tggtttactt
     7501 cgcaaccgga gcacgccagc aatactgaac cgcaggttac cgtcagtgtt ccggtagaca
     7561 gtaataacta ctggtatggc gtgctgggga tgagtattcc cgtgcgtacc atgcagcaat
     7621 ttttaagaaa cgccatcgat aaaaacctcg atggtgagta tcagctctat gacagtaagc
     7681 tgagattttt gacctcttcc aatcctgacc atccaacagg gaatattttt gatcctcgtg
     7741 aactggcctt gctggcgcag gcgatggaac atgacacgcg gggcggcatt cgtatggaca
     7801 gtcgctatgt tagctgggaa cgtctggacc atttcgacgg tgtgctggtg cgcgtccata
     7861 cgctaagcga aggcgtgcgc ggcgatttcg gcagtatcag cattgcatta accctgctgt
     7921 gggcgctctt taccaccatg ttactcatct cctggtatgt gattcgccgg atggttagca
     7981 acatgtatgt tctgcaaagc tcgttgcagt ggcaggcgtg gcacgacacc ttaacccgtt
     8041 tatataatcg tggcgcactg tttgaaaaag cccgtccgct cgcgaaattg tgtcagacgc
     8101 accaacatcc tttttctgtc attcaggtcg accttgacca ttttaaagcg attaatgacc
     8161 gctttggtca tcaggcgggc gaccgtgttc tttctcatgc tgccggatta attagcagtt
     8221 ccttgcgtgc gcaggacgtt gccgggcggg tcggtggtga ggagttttgt gtgattctgc
     8281 caggcgcgag tctgacggag gctgcggaag tcgcagaacg tattcgcctg aagttaaatg
     8341 aaaaagagat gttgattgct aagagtacga cgatacgcat cagtgcctcg ttgggggtaa
     8401 gtagcagcga ggaaaccggt gattatgatt ttgaacaact ccagtcactg gctgaccgtc
     8461 ggctttatct cgctaaacag gccgggcgta atcgggtatg cgcgagcgat aacgcttaac
     8521 gagcggagaa aaaatggtcc agcccttcac gccagccttc cggtccttca cgctgcgttc
     8581 gccagacgcg ggtcggatcc tcatcatgca gatgcacccc ttcacggttt agccctttca
     8641 caatcaccgc gtaatccatt acctccagta agggcgcatc gtttggccca tcgcccaggc
     8701 caagtgtggt tgggcgtttg cctgataatt gttgataggt cgcgataatc cagttggcgg
     8761 cctgatcttt tccggcagag gcatccagga cgtgccagaa gcgcgcaccc tgcataaact
     8821 gtaagcccag ttcgttcaga cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc
     8881 agattagcgt taccgacgcc tcatgcagct gcgtcagcgc cgcctggcta cggcttaatc
     8941 ccgtccattc ggcgatggtt gcatcgtcga catcatcgaa agtcgtgaat ttaaaatgtt
     9001 ctttctcgcg tagcgtattt aaaacctggc tgatttcgcc atggctaata cctgagatga
     9061 tgcgtggaaa accgtctata tcctgccatt gttcagcaag ctggatcact gcgccatttt
     9121 cggcaatcag tggtaaccct tgtagcccca acgttttttg caagtacagc atttccgctg
     9181 aagttttact gctacagaga atgacgggaa cattcgcttc gcgtaaacgg ctgagccaga
     9241 gggcagtcgg ttgccagtca taactatgac tgtccagcag ggtgccatca agatcgctaa
     9301 aaaccagtag tggttgttga attgaaaaca tggtcgtgca gttctcccct taccaggatg
     9361 attctcaaaa cagcacaaat aatcaaagat gattttttcg ggagcgatcc cgttgacgcc
     9421 agagccacca aggggtgacg cacgtcacat ttctattcat aagtagcgtt aattattcat
     9481 atcgcgaata ttttcttgtc agcgaaaaaa attgcggata aggtgatgaa cacatcagat
     9541 ttcctggtgt aacgaatttt ttaagtgctt cttgcttaag caagtttcat cccgaccccc
     9601 tcagggtcgg gattttttta ttgtgcattc aacgattcac ttcagagacg ctgaagtcat
     9661 gaatatccag ttcgaaggca tccgccaatt cacgccatgt atgatattca cgtccatcga
     9721 cgctaacgtg ttcagaatcg ttctggctgc gatgaacttc gctttcgcta atttcattga
     9781 tcgccgccag cagggcatcg acatcgacgc tgacctcacg ttttgcggta tcgctgtact
     9841 cttttgcggt tttcatcatc tttcctcgca accgttactc tgttgttgcc gccagtcgtt
     9901 aagtccgcct ggcatctctt aagtatagac cgtcgagaaa atcagcactc gcgcgcgcct
     9961 ggagctgcca gaaaaacgaa attgttctac actggcacaa agccacagga ggaaaacgat
    10021 gaaggtgaat gatcgggtaa cagtcaaaac ggatggcggt ccgcgtcgtc ctggcgtggt
    10081 actggcagtt gaggagttta gtgaaggcac aatgtacctg gtttcgctgg aagactaccc
    10141 gctcggcatc tggttcttta atgaagcagg gcatcaggac ggtatctttg tggagaaagc
    10201 agagtaatct tattcagcct gactggtggg aaaccaccag tcagaatgtg ttagcgcatg
    10261 ttgacaaaaa taccattagt cacattatcc gtcagtcgga cgacatggta gataacctgt
    10321 ttattatgtg ttttgatctt acgtttaata ttacctttat gcgatgaaac ggtcttggct
    10381 ttgatattca tttggtcaga gatttgaatg gttccctgac ctgccatcca cattcgcaac
    10441 atactcgatt cggttcggct caatgataac gtcggcatat ttaaaaaaga ggttatcgtt
    10501 gtctcttttt tcagaatatc gccaaggata tcgtcgagag attccggttt aatcgattta
    10561 gaacttatca ataatttttt tctgaccaat agatattcat caaaatgaac attggcaatt
    10621 gccataaaaa cgataaataa cgtattggga tgttgattaa tgatgtgctt gatgtgctga
    10681 ctgttagaag catcatggat gaaacagtcc tcattaataa acaccactga agggcgctgt
    10741 gaatcacaag ctatggcaag gtcatcaacg gtttcaatgt cgttgatttc tcttttttta
    10801 acccctctac tcaacagata cccggttaaa cctagtcggg tgtaactaca taaatccata
    10861 ataatcgttg acatggcata ccctcactca atgcgtaacg ataattcccc ttacctgaat
    10921 atttcatcat gactaaacgg aacaacatgg gtcacctaat gcgccactct cgcgattttt
    10981 caggcggact tactatcccg taaagtgttg tataatttgc ctggaattgt cttaaagtaa
    11041 atgttgcgat atgtgagtga gcttaaaaca aatatttcgc cgcaggagta tcctggaaga
    11101 tgttcgtagg tattattttt cggataatcc ttaggataac atgataaacg ttacggaatt
    11161 atattaatgg caattcacta ataatatcag ccagcaaatt aaacatctca ctgaataaat
    11221 gttcgcaaaa aggtgcaatt aacggcatta atgccgccat taaagagata ccgacagtca
    11281 gagttaatgg aaatccaata acaaaaatgg ataattgcgg ggccatacga ttcagtaaac
    11341 ctaatgccag attcagggtc agcagcagag taatgagcgg taacgccagc atcagcccgt
    11401 taaggaaaat caaactcccc gctttggtga gtgccagaaa cgcattgctg ttcaacggtt
    11461 cgccaccaat tggcagggtg tgaaaggtat cgaccagcag tgaaatcaac cataaatgac
    11521 cgttaaatgt caggaacagc agtaacgcca gcatatccat gatacgcgct aaaacgggca
    11581 tattgagatg gctggccgga tcgacaaacg tcgcaaatga cagccccatt tgcagaccga
    11641 taatttcgcc agcggttcgc acagcggcaa aggcaaattg catggtaaaa ccaagcgcaa
    11701 tgccgatcag gatctgctgc acggccagcc acagagcaaa gaacgaaaaa acaggaacat
    11761 cgttggcagg taatgatggg gcaatgacga acgtgatcat cattgccagc cccagtttta
    11821 cccgtttcgg cacgctgcgt tcgctcagaa ttggtgcggt agaaatgagc gccagcacgc
    11881 gcagtaacgg ccagaagtac aggcttaacc aggaaagcca ttgatcgctt gtcacctgca
    11941 tcattgtacg gcctacccga tgatatacgg caggttagtg aacaaggtgc ggacgtaatc
    12001 cagcaacaga ttgagcatcc acggtccggc aataataatg gcaataaata cggcgatgat
    12061 ttttggaata aacgacagcg tcatttcgtt gatctgcgtg gcggcctgca aaatactgat
    12121 gataaggccc gtgaccagcg ccaccagcaa cagcggggca gccagtgcca gcgcgacttt
    12181 catcgcttca gtccccatca tcatgaccga ttcaggtgtc attttgcctc tctagctgta
    12241 aaagctctgc gccagcgaac cgaccagcaa ttgccagcca tccaccagta caaacagcat
    12301 cagtttaaag ggcagagcaa tggtggcggg ggggaccatc atcatcccca atgccatcaa
    12361 cacgctggca atcaccaggt cgataatcaa aaaaggaatg aaaatcgtga agcctatctg
    12421 aaatgcggtt ttcaattcgc tggtcacgta ggccgggagc aaaatgcgca tcggcacggc
    12481 ttcaggtccc tgcaacgggc cggtattcgc tagtctggca aacaacccta aatctgcctc
    12541 acgggtctga cgcagcataa actcacgtag cggctgcgcc cctttttcca gcgcctcctg
    12601 cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt tgtcgatcac
    12661 cgatgacata ataaaaaagg tcaaaaatag tgccagcccc agcaatacct ggttaggtgg
    12721 cgcggagggc gttcccagcg cgttacgcaa taaaccaaaa acaatgatga tgcgggtgaa
    12781 actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga acaccagcgt
    12841 ctgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg tgatacccgg
    12901 cagttgcgcg aaggcgaggg gcgtaaccag ccagagaagg acaggtgcga cagacaacaa
    12961 acgacgcatc aggatctccc actacgctta agcaaatttt tcatgaccga ctgaaaatcg
    13021 gtctgcggta tctcttctgt tggtgctgaa gggggaagtt tatgcagcag attgatttgc
    13081 cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac aacccgttca
    13141 cgcgcgccca gcgaagcact ggcgctaatt ttcagaccgt taacgccagt gcgtttcggg
    13201 gcaaatccca accgttttac cagccaggca gcagcgagga tcagggcaat aatggcgatg
    13261 agtgcgccgc tgacctgcag cagtggcgca gcagaaaccg gcgtggaaga ttgcacagta
    13321 gcgtggttat tcatcactaa cggctcaggc gacgcattcg ctcagacgga gtaatgatat
    13381 cggtgatccg cacgccatat ttatcggcaa cgaccaccac ttcgccctgg gagattaaat
    13441 aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc acgacggacc
    13501 cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc tcgacggtca
    13561 gcttgaccgg aatatccatt atcaggtcga tatcctgcaa cgttccgctg acatcaccac
    13621 cgccaaattg ctggaacacc gcgtcggcag cgcttttgct gctggttgat ttttgttcgc
    13681 tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc ggattattca
    13741 tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa tcaaatgttc
    13801 tatccgtaac gcatactgac cgttgagagt gccgtactga ctggtcagca ccgggacgcc
    13861 gtcaacatgg gcgatgatgc catcgggttt ttctatcggc aggacgtcgc cgggtttcag
    13921 ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca gctccagctg
    13981 tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat tacgcgagtt
    14041 ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga atggcagaca
    14101 gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt taaccacaat
    14161 gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac gcacgtactc
    14221 aacttccagc ggattaatcg ccttccaggc gtcgctatag ccttcaagcg ccagctttaa
    14281 catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca ctttggtcgg
    14341 gaagcgtcca ttgccgccaa acaggttatc cacggcgata aacaccagac tcggtgaaaa
    14401 caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt tggtcggcac
    14461 cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga cggttatatc
    14521 cgggctacga cgcagcaggt tgaacagccc catacgaaaa tggcgggcaa agcgctcatt
    14581 aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat tcggatcgta
    14641 cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga cttcgctgtc
    14701 accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca tgtcgttatc
    14761 gcagaataaa agcggtatac atcacgtcgg tgacatcctg tttcggttgc ccggcaacaa
    14821 gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct tcttctgtcg
    14881 ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa acggctacgg acttccggca
    14941 aatactcact cagccgcgag cgggtagctt catctttgag tcgcagggtt ataccgatat
    15001 aaagcacgcg atccgcatcg cccaaattga ccgtaaaggt atccagcgca tagaagaccg
    15061 gtgagggcac gacgcgatgc tgcgctttgt cttcagcggc aacctgatgc gaatgccagt
    15121 agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc gggagccaaa
    15181 gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt cttcctgtgt
    15241 cgctactgct tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg ggaaaagacg
    15301 cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt tgcctgttac
    15361 gcgcccttgt aaagagacgg gaaccggaag cgtatcgtct tcttcccccg ccagaggttc
    15421 atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct gaccactaaa
    15481 gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctctcggcca gctgcgtacg
    15541 cagtaccggc agcgctgctt ccagggcggc gcgtacgtgc tgatgcggcg aaaccatctg
    15601 gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta aatcctgcgg
    15661 gtgcagacgc aactctgcac tttgttgtcc ctggcgggtg aacagcgaaa tatgctggct
    15721 taatgattgt tgccattcgt gagaacccag cggtgcactc aagacaggcg cggcgacggt
    15781 gagcagtggc tgtgtctggt gtggagtgat tagcgggctg gcggcagcgg tcaccggtga
    15841 aggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg ttaatggctg
    15901 agctggcgtg ccgggggcat catcaggctg tgctgttgtg agttgcgcag aagtcagttt
    15961 tgtgaagagc gttggtttct ctgccggtaa cacggttgac ggcgcatcag tcactttggg
    16021 cgtattgtca aaacccggca acatcgcaaa aagggcgctc aggcttgcgg tgaggtcttc
    16081 attcagatca tccgcttttt cgtcttttgt cgtatttttg tcagccaccg cagccaacgt
    16141 catcgtatga gcggtggtta acggtgtcga tgtggattgt tcgacgttga tgacaggcag
    16201 tgtttcatcc acagggatca gtaaatccgc ttgttgcgcg tcggaaacaa tatcgctgat
    16261 cagcagctcg ccttttgtcg tgggcttatc tgttgccacc agcacctggg gggccgcttt
    16321 gtcggtagtt gtctcgcttg ctaatgcttc gctcaacaac gcgagaaaat cttgtgcagc
    16381 atcgctggct ttgccgccag gcaatgtggt ggtgtcaacg tcggcggtaa tcaagggcgc
    16441 taagcgaatc attcaggttt cctcatggcg gcgcgctggg cgaactcatc catctttttc
    16501 tgatcgaggc ggttttctgc aagcagtgcc gccgtggatt gccgttcctg cagtgtctgc
    16561 caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc aactttctgc
    16621 gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt ctggataaac
    16681 tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct gttgaggtta
    16741 ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc ctgctgacac
    16801 ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc tgccagatct
    16861 ttcagggtcg ccagcgcacc atgttctgcc atctaccgtt atctcctggg ttatgacact
    16921 gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcacg ttcaaaaatg
    16981 ccttgttgta aatagccctc cagctgcggc cacagggcga tggctttatc gagcatcgga
    17041 tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg aaaactcgac
    17101 aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa cgccgtcatt
    17161 gcgcggctga tcgacgcttc aatatcgata gccggatagt gcccggcttc cgccagtcgg
    17221 cgagacagca caatgtgacc gtcgaggatc gcccgtgcgg agtctgcaat ggggtcctgc
    17281 tggtcatcgc cttcagtaag cacggtataa aacgcggtaa tcgagccgcc gccgctaatg
    17341 ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg tggataacct
    17401 ttggtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat cgcgtagcgg
    17461 gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc ttcggcaatg
    17521 cgcgtggcat aggcggcacc ttgcattcgc agaagcggag agacatccgc cggagcggca
    17581 atcaccacgg agcgtgcacg cccttcagca ccgaggatat tctcaataaa atcttttact
    17641 tcgcgcccac gttcaccaat caaacccacg acaatgacat cggcgcgggt gtaacgtgcc
    17701 atcatcccca gcagcacact tttaccaacg ccggacccgg caaacagccc catacgctgc
    17761 ccacgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag cacatgttca
    17821 atcggtgtac gttgcaacgg gttaaatggc ggggtaatca gcgcaccggt ttccgtcgta
    17881 tcgggggagg gcaggccatc gagcggctta ccgctgccgt ccagaacgcg acctaataac
    17941 gccggaccga gcggcaactg cttgccgctt tgcagtcctt cagccgaaat gtttttggca
    18001 taaacccgcg cgccgggcag gacaccttcc acttcctcca gcggcattaa aaacagccgt
    18061 tgaccgttaa agccaacgac ttcgctttct acttcgtgcg tttcgctgcc gttctggcgt
    18121 tcaatgacac aggttgcgcc gagcggcaat tgtaatccgg tggcttccag caccagcccg
    18181 gtagcgcggg ttaatcgccc atagcgacgt accgcaggca actgcgccat tttggcttca
    18241 aagttatcca gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc actcctggtg
    18301 ctgccagacg gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat
    18361 cggcggagac tttacagccg ccaggatgga gtgtgggatc gccccgcaag cgccagccat
    18421 gcaaacttaa ggttgcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc
    18481 gcagctgtgg tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga
    18541 tcagtgccga gttatccact gttggcgtct gaccgatcac ctgacgtgcc gcctccagcg
    18601 ccatctgcat caggcgcgaa gcaatcacac tatcaagcgc atcaagggta gtttgaaatt
    18661 cgctgaccag ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca
    18721 gaccttgctc cagcccctgg gccagtcctt cctgatagcc ctgctcatga ccttgctggc
    18781 gaccttcggc aatccccgcc tgataacctt gctcatgggc ctgcatttgc agttgcgcca
    18841 gttgctgctc aaggctaggt tcggcctctt caatgatggt ttcttccggc tcgaccatgg
    18901 gcacaaactc tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cacggcagat
    18961 tatcagacat aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg
    19021 cgcacaatca gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca
    19081 cggttggcga gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat
    19141 ttctcgcgca gtggctgctc ggcccctttc agcgcgatca acagcgattc ggaatccact
    19201 tcctgcaaca gacgctgaat gctgcgatcg tcgacatcca ccagattttc gaacaggaac
    19261 atctcgtcga taattttttg cgccagctcg ccgtcgaatt cgcgcacggc ggtaataacg
    19321 gcttcttcct gctgagtttt catcaggttg ataatttcgg ctgccgttct cacgccgccc
    19381 attttgctgc gcttgagatt ctgaccgtcg agcaagccat tcagtacttc ggtcagttcc
    19441 gccagcgcgg ctggctgcac gccgccaaag gtggcgatac gcaacatcac gtcgtggcgc
    19501 agacgttcat cgaacaacgc cagaatatcg gcggcctgcg cgcgcttcag atgcaccaga
    19561 atggtggcga taatttgcgg atgctcatcg cgaatcagat cggcggcact ctgcggctcc
    19621 ataaagttga gcgtttcaat accgctggcg gtatcgcgag tttcgagaat atcttccagc
    19681 aggctggcgg cacgttcctc acccagagct ttgaccaata ccgaacgcag ataatcgttg
    19741 gcgttgatat tcagtgcggc aaactgttca gcttcttgct caaactccgc cagcacatag
    19801 gttagctgct tgttggagat ctgcgtgacg ttcgccattg cagcgctcag ggtttgcact
    19861 tcgcgctggg agaggtgctt gaacacctct gccgcccggt cttcgccaat ggtcatcagc
    19921 aggatgacgc ttttatcggt gcctgtcagg ttactcatga tcgttattta tccactggcg
    19981 aatgaccagc gccaccacgc gcggatcgtt atcagacatt tcacggatac gctggctcat
    20041 gacttctgcc cccagacgtt ggttagcgcg tcgttgttgg agttgttcgt ctttgctcag
    20101 gcggacttcc accgcatctt ccacttcctc acgggcctgc gcctgttgct gtacagcttt
    20161 catcgcctcg gcgcgacgtg ttaactgcgg gcgtaccgct ttccgccaca gcagccacgc
    20221 caccagcagt accagcaacc agcgaccggc agccagcaac tgatcgataa acgcttgctg
    20281 ttgccagaac ggcagttctc cgccgctttc gtcactgcta ttgaacggcg agttaacgac
    20341 attgagcgag tcaccgcgtt tttcagaaaa gcccatcgct tcgcgggtca gatcttcaat
    20401 ttgcttcatc tgttcgttgc tgagaggcaa tggtttaccg tctggcaagg ttttgtaatt
    20461 caccactacc gcgactgaca gacgttgcac atcgcccacg ttcattttgg tatgacgaat
    20521 ggtgcgatcg acttcgtagt tactggtttc attccgctgt gtgctacgcg gcccgctatt
    20581 gctggtggtg ctcgcctgct gctggcggtt attttgattt gccggaggcg tgctgattgg
    20641 cgcgttattc gcaggtgccg gttgattcga caacgccccc ggtacgccgc ccggataacc
    20701 ggaaccgctt tgctcgctct cattaagctg gcgtgaacga agcgccgcat gagattcatc
    20761 accgttaggg cgatactgtt cttccgtttg ttctttactg gcgaagtcca gttgcgccgt
    20821 gacctgggcg tgaatattac cgttaccaac aataggcgac aggattgctt caatacgccg
    20881 ctgaatacgg ccttcgacat cgctggcata tttcaactga gcgtcattaa gatcgcgccc
    20941 gctggtattg gactgggtta acagatgtcc gccctgatcc accagcgtga cgtttcccgg
    21001 cggcagacca gcaacggcgc tggaaaccag gtgcacaatg gcgctaattt gcccttcatc
    21061 gagtgcgcgg ccgggtaaca gatttaccgt caccgatgca gaaggggatt tttgttcacg
    21121 gacgaataaa gacggtttcg gcattgccag atgtacgcgc gcccctttta ccgggccgat
    21181 agtttcgatg gtacgagaaa gctcgccttc cagcgcccgc tgataattca cctgttcgct
    21241 gaactggctg ataccaaact tttcctgatc aagcagttcg aaaccgaccg cgccgccttt
    21301 gggcaaacct tgttgtgcca ggcgcagacg cagttcgtga actttatctg ccggaacttc
    21361 aatagcgccg ctggcttcgc tgaagcagta aggaatattc atttgcgtca gttggctgac
    21421 aattgcgcca ccatcctgat cggaaagatt gctgaataat gtgcggtagt cgggggcttt
    21481 cgcccacagg atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa caatcaatgg
    21541 aattttcgga ttcgcacgca ggcgattaag ccactcaaga gattttgtct gggctgcagt
    21601 cgcattcatc gcgcacctcg tggctgatta tggacggcgt tattggcaaa gtggaacaag
    21661 actcactccc gggtcagcaa actcgaaaaa ttgacgctgc cattatttgt gctttcgttt
    21721 ttttctatcc gccaataaac ccgttttttt tgttgctatt tagcgccttt gtcttattga
    21781 cttactggta agctttgcta ccagaaatta tccgggagat gaaaatgtca gcgatacagg
    21841 ggattgaagg ggttatcagc cagttacagg ctacggcgat gagtgcgcgt gcgcaggaat
    21901 cactgccaca accgaccatt agttttgccg ggcagctgca cgccgcgctc gatcgcataa
    21961 gcgatacaca aacagccgcc cgcacgcagg cagaaaaatt cactctcggt gaacccggcg
    22021 tggcgttaaa cgatgtgatg accgatatgc aaaaagcctc agtttctatg caaatgggga
    22081 ttcaggtgcg taataagctg gtggcggctt atcaggaagt gatgtccatg caggtgtagg
    22141 ttttgtaacc tgttgttaat tacatccgat ctcaccgcgt ggggcatgga tggggcaaac
    22201 tcactcaatt tctggtttaa aatggatacc tgatcctgaa agttttctgc catccacgat
    22261 ccgtaaacgc gataacatgt tttgtcttcg ctttgctcat aggaagagtg aactcttttg
    22321 ttagcctggg gttccttctg atcattattg ttcctgcctt tatctcttgc cacgccagag
    22381 ccccctggat acacatacac caggatgaaa acggtgagtt gtgttcaaac tgtagtacaa
    22441 ttctctccag tatgaacagg aaagaatatg ctatgaaccc ttatatttat cttggtggcg
    22501 caatacttgc agaggtcatt ggtacaacct taatgaagtt ttcagagggg tttacacggt
    22561 tatggccctc tgttggcaca attatttgct attgtgcatc attttggtta ttagctcaga
    22621 cactggctta tatacctaca gggattgctt atgctatctg gtcaggagtc ggtatcgttc
    22681 tgattagttt gctttcctgg ggattttttg gccaacgact ggacctgcca gccatcatag
    22741 gcatgatgtt gatttgtgcc ggtgtgttag tcattaactt gttgtcacga agcgcaccgc
    22801 attaaaaaat aatcatcctt gagataacag gaatctgatt tgtgcatatt atggctaatg
    22861 ttcggggcaa ctttataatt atgattgcat ttgcgcgatc attgcgcctt tttgcataat
    22921 gagatcaatg cacatcaaat aaaataacga taggtcacaa caaagaggtt tttatgaaaa
    22981 aacttatcgt ttcaagtgta ttggcattca taacattttc tgcgcaggcc gcagcatttc
    23041 aggtcactag taatgaaata aaaacaggag agcaacttac aacgtctcat gtattttctg
    23101 gagttgggtg tgaggggggt aatacatcgc cctcattaac ctggtctggt gctccagaag
    23161 gcaccaaaag ctttgccgta actgtgtatg atccagatgc gcctacaggc agcggatggt
    23221 ggcactggac tgttgctaat attccagcaa cggtaacata tttgcccgct gatgcaggaa
    23281 gacgtgatgg tacaaaactt ccgactggtg ctgtccaagg ccgaaatgat tttggctatg
    23341 cgggatttgg tggagcatgt cctcccaaag gagataaacc ccatcattac cagtttaaaa
    23401 tatgggctct aaaaactgat aagattcctg tggattctaa ttccagcgga gcgttagttg
    23461 gttatatgct taatgccaat aaaattgcaa ccgctgagat aatacccgtt tatgaggtaa
    23521 agtaggataa gagtatgctg gcaaaagata ggactaacct aaagattgaa gaaatacgga
    23581 tgcataaaca tcatgagatt cacagggtta agcctcttat gccagcttca tgtcgtatcc
    23641 gtcagggaaa aaaagttatc aattgggaga cgcatagtct aactgttgat aataatcaga
    23701 taatattatt tccctgtgga tatgaatttt atattgcgaa ttacccagaa gcaggtcttt
    23761 atcttgcaga aatgctttat tatcccattg atctaattga aaagttccaa aatctttatg
    23821 cgataaccga tcaaattcgt aacacgacag gtttctgctt acctcagaat tccgagttaa
    23881 tatattgttg ggagcaacta aaaacatcta tttcccgagg cttctcaacg caaattcagg
    23941 aacacttagc aatgggcgtt ctactttcat taggagcaca tcatgttaat tgtttacttt
    24001 tatcagatag taaacaatca ttaataagtc gttgttataa cctaatgcta tccgaacctg
    24061 gaacaaaatg gacagcaaac aaggtagcga gatatctcta catttctgtt tccacattgc
    24121 atcgccgtct ggcaagcgag ggagtaagtt ttcaaagtat attggacgac gtgaggttaa
    24181 ataatgcgtt gtctgctata caaacgacag taaaacccat cagcgagatt gccagggaaa
    24241 atggttacaa gtgtccttct cgttttactg aaaggttcca taatcgtttt aagataacac
    24301 caagagagct aagaaaagcg tccagagagt aaaagtattt tatgaaggag caacagcatc
    24361 gatttttatt ttggaaaata aatacggcat actcaaccta ttggagcctg aacaatccgg
    24421 tgacttctgc gctaatcggg gacgtttata agcacatata attgatacta tcttcctcca
    24481 tcacaatttc agaaatgcac gtgtgatttt tttgcatccg gcgtttggac tatgtggagt
    24541 tcaagagtgg gaaagttaca agaggttgag gggataatta aagggatgct tatgacatta
    24601 gatatccgga taggtacgga tatcgttgtt attaaatcta gttcgttatt attaaatcta
    24661 gtttatacgt taagatgcag ctacagcaga aattgccact gttaattttt tcatcgtgag
    24721 cccttttatt tgaactatta tttaaaaatg atgtcactgc actacaaata ttcatccaat
    24781 caatgagatt atttcaagat gtaagtttta gtttctcatt taaattgtga agtagat
//
LOCUS       DAJTVJ010000058        24642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000058 DAJTVJ010000000
VERSION     DAJTVJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.058"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(132..1781)
                     /gene="actP"
                     /locus_tag="OO448_002262"
     CDS             complement(132..1781)
                     /gene="actP"
                     /locus_tag="OO448_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6337457.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1778..2091)
                     /locus_tag="OO448_002263"
                     /pseudo
     CDS             complement(1778..2091)
                     /locus_tag="OO448_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2291..4249)
                     /gene="acs"
                     /locus_tag="OO448_002264"
     CDS             complement(2291..4249)
                     /gene="acs"
                     /locus_tag="OO448_002264"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6337458.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4643..6079
                     /gene="nrfA"
                     /locus_tag="OO448_002265"
     CDS             4643..6079
                     /gene="nrfA"
                     /locus_tag="OO448_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6337459.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6124..6690
                     /gene="nrfB"
                     /locus_tag="OO448_002266"
     CDS             6124..6690
                     /gene="nrfB"
                     /locus_tag="OO448_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6337460.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6687..7358
                     /gene="nrfC"
                     /locus_tag="OO448_002267"
     CDS             6687..7358
                     /gene="nrfC"
                     /locus_tag="OO448_002267"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6337461.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7355..8311
                     /gene="nrfD"
                     /locus_tag="OO448_002268"
     CDS             7355..8311
                     /gene="nrfD"
                     /locus_tag="OO448_002268"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6337462.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8331..10049
                     /locus_tag="OO448_002269"
     CDS             8331..10049
                     /locus_tag="OO448_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6337463.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10042..10425
                     /gene="nrfF"
                     /locus_tag="OO448_002270"
     CDS             10042..10425
                     /gene="nrfF"
                     /locus_tag="OO448_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6337464.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10422..11018
                     /gene="nrfG"
                     /locus_tag="OO448_002271"
     CDS             10422..11018
                     /gene="nrfG"
                     /locus_tag="OO448_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6337465.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11360..12673
                     /gene="gltP"
                     /locus_tag="OO448_002272"
     CDS             11360..12673
                     /gene="gltP"
                     /locus_tag="OO448_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6337466.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12752..13441)
                     /locus_tag="OO448_002273"
     CDS             complement(12752..13441)
                     /locus_tag="OO448_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6337467.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13535..15682)
                     /gene="fdhF"
                     /locus_tag="OO448_002274"
     CDS             complement(13535..15682)
                     /gene="fdhF"
                     /locus_tag="OO448_002274"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15263..15265),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6337468.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15880..17346)
                     /gene="mdtP"
                     /locus_tag="OO448_002275"
     CDS             complement(15880..17346)
                     /gene="mdtP"
                     /locus_tag="OO448_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6337469.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17343..19394)
                     /gene="mdtO"
                     /locus_tag="OO448_002276"
     CDS             complement(17343..19394)
                     /gene="mdtO"
                     /locus_tag="OO448_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6337470.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19394..20425)
                     /gene="mdtN"
                     /locus_tag="OO448_002277"
     CDS             complement(19394..20425)
                     /gene="mdtN"
                     /locus_tag="OO448_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6337471.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20444..20719)
                     /locus_tag="OO448_002278"
     CDS             complement(20444..20719)
                     /locus_tag="OO448_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337472.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20928..22913)
                     /gene="yjcS"
                     /locus_tag="OO448_002279"
     CDS             complement(20928..22913)
                     /gene="yjcS"
                     /locus_tag="OO448_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR6337473.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23166..23483
                     /locus_tag="OO448_002280"
     CDS             23166..23483
                     /locus_tag="OO448_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR6337474.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23552..23881
                     /gene="yjdP"
                     /locus_tag="OO448_002281"
     CDS             23552..23881
                     /gene="yjdP"
                     /locus_tag="OO448_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR6337475.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(24028..>24642)
                     /gene="phnP"
                     /locus_tag="OO448_002282"
     CDS             complement(24028..>24642)
                     /gene="phnP"
                     /locus_tag="OO448_002282"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6337476.1"
                     /translation="AITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGV
                     GDPIPIYGPPDEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGY
                     LLETAHSRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLA
                     LNQVIRSPRVILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
BASE COUNT         5590 a         6454 c         6716 g         5882 t
ORIGIN      
        1 ttatcatttt ctacgactgt caaaaatcgc tcatttttta atgagtttat ttgtttaata
       61 ttttgggaaa aggtgatgca tgctggagag gaagagtatt ccccggtcag gcgaccgggg
      121 aaggggagag attaatgtgc gcggccttgc tcaacgccaa agccggtctg ggagcggata
      181 aactgcgcgc ggaacagttc acgctcgcgc gccccttccg ctgagttatc ggttgccgag
      241 aagaaccaga tgccgaggaa tgccacggtg atagagaaca gcgccgggta ttcatacggg
      301 aagatggctt tttcgtgacc aaggatctgt acccaaatcg tcgggccgag gatcatcagt
      361 actactgcgg taatcagccc cagccagcca cccatcatcg cgccacgcgt ggtcagtttc
      421 gaccagtaca tagaaagcag aatgatcggg aagttacagc tcgccgcgat ggcaaacgcc
      481 agccccacca taaaggcgat gttctgattc tcaaacagca cgccgaggat aatcgcaatc
      541 acgccgagga tcagtacggt gatttttgat acccgcagct cttcacgttc ggtcgcgcct
      601 tttttgaaga cgttagcgta caagtcatgc gaaaccgcgg atgcgcccgc cagcgtcaga
      661 cccgcaacca ccgcgaggat agtggcgaaa gcaaccgctg aaataaaacc gaggaacagg
      721 ttgccgccca ctgcattcgc caggtgaacg gccgccatgt tgttaccacc aatcagatgg
      781 cccgccgcgt ctttatattc cggattcgca ccaaccagca tgatcgcgcc gaagccgata
      841 ataaaggtca gaatatagaa gtagcccata aacccggtgg cgtagaacac gctcttacgt
      901 gcttcgcggg catcgctgac tgtaaagaag cgcatcagaa tgtgcggcaa gcccgccgta
      961 ccaaacatca gtcccagacc cagagagagt gcggagatcg gatctttcac cagcccgccc
     1021 ggcttcatga tgtcgacacc tttcgggtgt accgccatcg cttcactgaa cagattgttg
     1081 aagctaaagc cgacgtgttt catcaccata aaggccataa agctggcacc gaacagcaac
     1141 agcacggctt tgataatctg tacccaagtg gtcgccagca tgccgccgaa caggacgtac
     1201 atcatcatca gcacgccgac cagcaccacc gcaatgtgat agttaaggcc aaacagcagc
     1261 tcgatcagtt tacctgcgcc caccatctgg gcgataaggt aaagcgccac caccaccaga
     1321 gaaccacagg ccgaaagaat acgaatcggc ccttgtttca gacggtaaga ggccacatcg
     1381 gcaaaggtgt agcgccccag gttacgcaga cgttcggcaa tcaggaacaa aatgatcggc
     1441 cagcccacca ggaagcccag cgagtaaatt aagccgtcat agccggaggt aaacaccagc
     1501 gcggagatcc ccaagaatga ggcggcggac atatagtccc cggcaatcgc cagcccgttc
     1561 tggaagccag tgatattgcc gcctgcggtg tagtagtcgc tacgagaacg tacgagtttt
     1621 gatgcccagt aggtaatgcc gagcgtaaac acgacgaaaa tcaggaacat aataatcgcc
     1681 tgccagttcg ttggctggcg ctctacggcc ccgctaatag catccgcggc gttagctgcg
     1741 aaagggagtg tggcggcaag cgccgtcaga actcttttca tgatgcttgt acctcatgca
     1801 ggacttcatt attaagacgg tcgaattcgc cgttcgcccg ccagatgtag ataccggtga
     1861 gaacaaagga gatcacaatc actccaacac caattggaat accgcgtgtg acgctggtgt
     1921 tcggattcag cggcgtgcca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat
     1981 ataaactgcc agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa
     2041 ctccctgaaa tgcgcattgt cttctatccg ctgataaata gtgccattca tcacagattc
     2101 tccagaggta atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct
     2161 cgggtaaccc ttgccgaatg taggccggat aaggcgttta cgccgcatcc ggcaatcaat
     2221 gcctgatgcg acgctgtcgc gtcttatcag gcctacaaac cgttaccgac tcgcatccgg
     2281 caattgtggg ttacgatggc atcgcgatag cctgcttctc ttcaagcagc ttctcgacta
     2341 cgccaggatc ggcaagcgtc gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt
     2401 tgcgcagaat acggcgcata attttgccgg agcgggtttt aggcagggag tcggtccagt
     2461 gcagcacgtc tggcgtcgcc agcgggccaa tctctttacg cacccagttg cggacttctg
     2521 cgtacagttc tggtgacggt tcctccccgt gattaagcgt gacgtaggcg tagatcgcct
     2581 gacctttaat attgtgcgga atacctacta cggcggcttc ggcaatcttc ggatgcgcca
     2641 ccagcgccga ctcaatctct gccgtcccca gacggtgacc ggagacgttc agcacgtcgt
     2701 ccacacgccc ggtgatccag taatagccat cttcatcgcg acgcgcgccg tcgccgctga
     2761 aatacatatt tttgaaggtg gagaagtagg tctgttcaaa acgttcgtga tcgccaaaca
     2821 gcgtacgcgc ctgacccggc caggagtcgg tgatcaccag gctgccttcg gtagcgcctt
     2881 ccagcgggtt accttcgtta tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg
     2941 ccgaaccggc tttcagctcg gtagcgccag gcagcggcgt gatcatgaaa ccgccagttt
     3001 cggtctgcca ccaggtatcg accaccggac atttctcgtt gccgattttt ttccagtacc
     3061 actcccacgc ttccgggtta attggctcgc ccacggaacc gagaatgcgc agcgacgaac
     3121 ggtcggtacc ctcgatcgct ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg
     3181 cggtatagag aatattgacc tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg
     3241 gccagttggg tacgccttca aacatcagcg tggtcgcacc gcaggccagc gggccgtaca
     3301 gcaagtaact gtgtccggtc acccagccca catcggcggt gcaccagtag atatcacccg
     3361 gatgataatc aaagacatat ttaaaggtca gcgccgcgta caccagataa ccgccggtag
     3421 tatgcagcac accttttggc ttaccggtag aaccggaggt gtagagaata aacagcggat
     3481 cttcggcgtt catcttttcc gcctggtgct gatcgctcgc ttgctcaacc aggtcgtgcc
     3541 accacaggtc gcgcccttcc tgccagtcaa ttttcccgcc agtacgcttc agtaccacca
     3601 catgctctac gctggtgacg ttcgggtttt tcagcgcgtc atcaacgttt ttcttcagcg
     3661 gaatactgcg cccggcacgc acaccttcgt cggaagtgat caccagtcgt gagttggaat
     3721 caataatgcg cccggcaacg gcttccggcg agaagccgcc gaaaatcacc gaatgcaccg
     3781 cgccaatgcg ggcgcaggcc agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa
     3841 tcgccaccac atcacctttt ttaatgccca gctcgagcag ggtattggcg aagcggcaga
     3901 cgtcgcggtg cagctcttta tagctgatat gtttgctctg gctggcgtcg tcgccttccc
     3961 agatgatggc ggtacgatcg ccgttttctt gcagatggcg gtcaaggcag tttgccgcca
     4021 gattcagcgt gccgtcctcg taccatttaa tggacacatt accgggggca aaggaggtgt
     4081 ttttcacctt ctggtaaggt ttgatccagt caagaatttt tccctgttcg ccccagaagg
     4141 tatcaggtac gttaatagat tgttgataca tcgcctcgta ctgctgaggg tttatcaggc
     4201 aacggtctgc gatgttggca ggaatggtgt gtttgtgaat ttggctcatg cttttgttct
     4261 ccttgtaggg tgttaataat atgtggcata agcgttaaat gtaggggtat tggcagtttt
     4321 gtttagtatt tgggcgacag atcacgcaaa agtagaattg tgcaaataaa cggcagggta
     4381 atttttgaag gtcagcaaca aaagttgatt aattctttga ggaacatgca gttatgcatt
     4441 gctgttgaaa aagaggaaga tactaactaa ctctaaagtg gtattttaca tgcacttaca
     4501 attgattaaa gacaacattc acagtgtggt tatttgttac acataggggc gagcaatgtc
     4561 atgacagtgt aggtgcggtt actgtcgtga aaaacaataa aaaccgccat tgtaacaatg
     4621 gcgcaattcg gatgaagccc ctatgacaag gataaaaata aacgcacgcc gtatcttcag
     4681 cttattgatt ccttttttct ttttcacttc tgttcacgct gaacaaacgg cagctcccgc
     4741 aaaacctgta actgtggaag cgaagaatga aacctttgcc ccgcagcatc ccgatcaata
     4801 tctctcctgg aaagccacct cggaacagtc agagcgtgtt gacgccctgg cggaagatcc
     4861 acggctggtg atcctgtggg cggggtatcc cttctcgcgc gattacaaca agccgcgtgg
     4921 acatgccttt gctgtgaccg atgtgcgtga aaccctgcgt accggtgcgc cgaaaaacgc
     4981 tgaagatggt ccgctaccga tggcgtgctg gagttgtaaa agcccggatg tggcgcgtct
     5041 gatccagaaa gacggcgaag atggctactt ccacggtaag tgggcgcgcg gcggcccgga
     5101 aatcgtcaac aacttaggtt gtgccgactg ccataacacc gcctcaccag agttcgccaa
     5161 aggcaaaccg gagttaaccc tttcccgtcc gtatgcggct cgcgcgatgg aagccattgg
     5221 taaacctttt gagaaagccg gacgtttcga ccagcaatcg atggtttgcg gtcagtgcca
     5281 tgtggagtat tacttcgacg gcaaaaacaa agcggttaaa ttcccgtggg atgacggcat
     5341 gaaagtcgaa aatatggagc agtattacga caaaattgcc ttctctgact ggactaactc
     5401 cctgtcgaaa acgccaatgc tgaaagcgca gcacccggaa tatgaaacct ggacagcggg
     5461 cattcacggt aaaaacaacg tgacctgtat cgactgccat atgccaaaag tgcagaacgc
     5521 cgaaggcaaa ctctataccg accataaaat tggtaatccg tttgataact tcgcccagac
     5581 ttgtgcgaac tgccataccc aggacaaagc tgccttgcaa aaagtggtcg cggaacgtaa
     5641 gcagtcgatt aacgacctga aaatcaaggt tgaagatcaa ctggttcacg ctcacttcga
     5701 agcgaaagca gcgctggatg caggcgcgac ggaagctgaa atgaagccaa ttcaggacga
     5761 tatccgtcat gcccagtggc gctgggatct ggcgatcgct tcccacggca ttcatatgca
     5821 cgcaccggaa gaaggtttac ggatgctcgg tacggcgatg gataaagcgg cggatgcacg
     5881 caccaaactg gcgcgcctgc tggcgaccaa aggcatcacc catgaaatcc agatcccgga
     5941 tatctcaacc aaagagaaag cccagcaggc cattggcctg aacatggaac aaatcaaggc
     6001 cgagaagcag gacttcatca aaacggtgat cccgcagtgg gaagaacagg cacgtaaaaa
     6061 cggtctgtta agccaataac cccgttccgc ctcgcaaggg gcggaaaaca caatggagtg
     6121 aatatgagcg tattacgttc gttattaact gccggggtgc tggcgtcggg cctgttgtgg
     6181 agcctgaacg gaattaccgc tacccctgcg gcgcaggcat ctgatgatcg ctacgaagtt
     6241 acccagcagc gtaacccgga tgccgcctgt ctggactgtc ataaaccaga taccgaaggt
     6301 atgcatggca aacatgcttc cgttatcaac ccgaataaca aactgccggt cacctgcacc
     6361 aactgtcacg gccagccatc accgcaacac cgcgaagggg tgaaagatgt gatgcgcttt
     6421 aacgagccga tgtacaaggt tggagagcag aacagcgtct gtatgtcctg tcatctgccg
     6481 gaacagttgc aaaaagcgtt ctggccgcac gatgtccacg taaccaaagt ggcgtgcgcc
     6541 agctgccatt ccctgcatcc acagcaagat acgatgcaga cgttaagtga caaaggacgg
     6601 attaagattt gcgtcgattg ccacagcgat cagcgcacca atccgaactt taacccggcg
     6661 tctgttccgt tgcttaagga gcagccatga cctggtctcg tcgccagttt ctcaccggcg
     6721 tcggcgtgct ggcagccgtc agcggcaccg cagggcgcgt ggtggcgaaa acgttgaata
     6781 tcaatggggt gcgttacggc atggtgcatg acgagtcgtt atgcatcggc tgtaccgcct
     6841 gtatggatgc ttgtcgggaa gtgaacaagg tgccggaagg cgtctcgcgc ctgacgatta
     6901 ttcgtagcga gccgcagggc gaatttcctg atgtgaaata tcgcttcttc cgtaagtctt
     6961 gccagcactg cgatcatgcg ccgtgcgttg acgtctgccc gaccggtgcg tcttttcgtg
     7021 atgccgctag cgggattgtc gatgttaatc ctgatctctg cgtcggttgt cagtactgca
     7081 tcgccgcctg tccgtaccgc gtgcgcttta tccatccggt cacgaagacg gcggacaaat
     7141 gcgatttctg ccgtaaaacc aatttgcagg ccggtaagct gcccgcgtgt gttgaggctt
     7201 gcccgacgaa ggcgctgacg tttggcaatc tggacgatcc taacagtgag atttcgcaac
     7261 tgctgcgcca gaagcctact taccgctaca agctggcgct gggaaccaaa ccgaagctgt
     7321 accgcgtacc gtttaaatac ggggaggtga gccaatgacg cagacttccg catttcattt
     7381 tgaatcgctg gtgtgggact ggccgattgc catctacctg tttttgattg gtatttctgc
     7441 cggtctggtg acgctggccg tgctgttacg tcgcttctac ccgcaggcgg gcggtgcaga
     7501 cagtacgttg ctgcgcacca cgctgattgt cgggccgggc gcggtgatcc tcggtctgtt
     7561 gatcctcgtc ttccacctga caagaccgtg gaccttctgg aagctgatgt tccactacag
     7621 ttttacctcg gtgatgtcga tgggggtgat gctgtttcag ctctacatgg tggtgctggt
     7681 gctgtggctg gcgaaaatct ttgaacatga tttgcttgcc ctgcaacaac gctggttgcc
     7741 gaagctgggg atcgtgcaaa aggttctgag cctgctgacg cccgttcatc gcggactgga
     7801 aacgctgatg ctggtgttgg cggtgctgct gggggcttat accggctttc tgctgtcggc
     7861 gctgaaatcg tatccgttcc tcaataaccc gatcctgccg gtgctgttcc tcttctccgg
     7921 catctcgtcc ggtgcggcgg tggcgctgat cgccatggcg atacgccaac gcagtaaccc
     7981 gcattccacg gaagcgcact ttgttcaccg gatggaaatc cccgtggtat ggggtgaaat
     8041 cttcctgctg gtggcgtttt ttgtcggtct ggcgctgggc gatgacggta aagttcgtgc
     8101 gctggtggcg gcattaggtg gcggtttctg gacgtggtgg ttctggcttg gtgtcgccgg
     8161 gctggggctg attgtgccaa tgttgctcaa accgtgggtc aatcgcagtt ccggcattcc
     8221 tgccgtgctg gcggcgtgtg gggccagtct ggtcggcgtg ttgatgctgc gctttttcat
     8281 tctctacgcc ggacagttga cggtggcgta agccagaaaa gaggtggttt ctggacgtat
     8341 tccttcctga agtcggtttt ctggcgttgt tgttaagtct cggggtcaac gtgttgaccc
     8401 cgttgacggc ctttgcggga gtgcggttgc gctggcctgc catgatgcga ctcacttgca
     8461 tcggcattct ggcgcagttc gcgctcctgc tgctcgcctt tggcgtactg acgtattgtt
     8521 ttctcatcag cgatttctcg gtcatttatg tcgcgcaaca tagctatagc ctgctgtcgt
     8581 gggaattcaa gctggcagcg gtgtggggcg gccatgaagg ttcgctgctg ctttgggtgc
     8641 tgctgctttc cgcctggagc gcgctgtttg cctggcatta tcggcagcaa accgatccgc
     8701 tatttccgct gacgttagcc gttttatctc tcatgctcgc cgcactgcta ctgtttgtgg
     8761 tgctgtggtc cgatcccttc gtgcggatat ttccaccagc aatcgaaggc cgcgatctca
     8821 atccgatgct gcaacatccc ggtcttatct ttcatccacc gctgctttac cttggctatg
     8881 gcggtttgat ggtagcggcg agcgtggcgc tggcgagtct actgcgcggc gagtttgatg
     8941 cggccagcgc ccgaatttgc tggcgctggg cgttacctgg ctggagtgca ttaacggcgg
     9001 ggatcatcct cggttcctgg tgggcctact gcgaactcgg ctggggcggc tggtggttct
     9061 gggatccggt ggaaaatgcc tctttattac cctggctttc tgccactgcg ctgctgcaca
     9121 gtttatccct gacacgccag cgggggattt tttgccactg gtcgctgttg ctggcgatag
     9181 ttactctgat gctgtcgctg ctgggcacct taattgtccg ttctggcatt ctggtttcgg
     9241 ttcatgcgtt cgcactggat aacgtccgcg ccgtgccgtt gttcagcctg tttgcactga
     9301 ttagccttgc gtctttggct ctgtatggct ggcgagcgcg ggacggtggc ccggcggtgc
     9361 gtttttcggg gttatcgcgg gaaatgttaa tcctcgctac gctgttgctg ttttgcgcag
     9421 tgctactgat cgtgctggtg ggaacgcttt atccgatgat ttacggtctg ctaggctggg
     9481 gacgcctctc cgttggcgcg ccgtatttta accgcgcgac gttaccgttt ggtctgttga
     9541 tgctggtggt gattgtgctg gcgacgtttg tctctggcaa acgcgcgcag cttccggcgc
     9601 tggtcgctca tgcgggcgtg ctgttatttg ccgcagggat cgtggtttcc agcgtcagcc
     9661 gtcaggagat cagcctgaat ttacagccgg gtcagcaggt gacgctggca ggatacacct
     9721 tccgttttga gcgcctcgat ctgcaagcca aaggcaatta caccagcgaa aaagcgatag
     9781 tggcgctgtt tgaccatcag caacgcattg gtgaattaac gccggagcgg cgtttttatg
     9841 aagcacgccg tcagcaaatg atggaaccgt caattcgctg gaacggcatc catgactggt
     9901 atgcggtcat gggtgaaaaa accggagcgg atcgttacgc ttttcgtttg tatgtacaaa
     9961 gcggtgtgcg ctggatctgg ggtggaggat tgttgatgat tgcgggcgca ttgttaagcg
    10021 gatggcgggg gaagaagcgc gatgaataaa gggcttctca cgctactgct gttatttacc
    10081 tgttttgcgc gcgctcaggt cgtagacacc tggcaattcg ccaatccgca acaacagcaa
    10141 caggcgttaa atattgccag ccagttacgt tgtccgcagt gccagaatca aaacttactc
    10201 gaatccaatg cgccggtggc ggtcagtatg cgccatcagg tttatagcat ggtgtcggag
    10261 gggaagaacg aagtcgaaat cattggctgg atgaccgaac gctacggaga ttttgttcgc
    10321 tataacccac cgttaacggg tcagacgctg gtgttgtggg cgctgccagt ggtgttgtta
    10381 ttgctgatgg cactgatcct ctggcgagtg agggctaagc gatgaaacag cctcaaatac
    10441 cggtgaaaat gctgacaaca ctcacgatat tgatggtatt cctctgtgtc ggcagttatc
    10501 tgttaagccc aaaatggcag gcggtacgtg cggagtatca gcgtcagcgc gatccgctac
    10561 atcagtttgc cagccagcaa accccggaag cgcagcttca ggcattgcag gataaaatcc
    10621 gcgctaatcc acaaaacagc gaacaatggg cgttactggg cgagtactat ctgtggcaaa
    10681 acgattacag caattcgctg ctggcgtatc gtcaggcgtt gcaactacgt ggtgagaacg
    10741 ctgaactgta tgcggcgctg gcgacggtgc tttattacca ggccagccag catatgaccg
    10801 cccagacttg cgcaatgatc gacaaagccc tcgcgctgga ctctaacgaa atcaccgctc
    10861 tgatgctgct ggcttccgat gcgtttatgc aggcgaatta cgcgcaagcc atcgaactat
    10921 ggcaaaaagt gatggatctc aactcacccc ggatcaaccg gacacagctg gtcgagtcga
    10981 ttaatatggc aaaactgttg cagcggagat cggattaatc atctggtgat gaagattatg
    11041 tcattgagct gcataaaaaa taatcgaatg aacatatgcc aaaaataatc actaatcagt
    11101 attattgcag attaaacaaa taaaaatctt tccataacaa atggttattc attaatcctg
    11161 ccataaactc gcgttattat gcattaatgc agcgaaaagc tctgttgtta aagggttgcg
    11221 caacataccg cgcaaatgat actgatcata agcgttaaaa aaatctacaa accaacgcaa
    11281 cacaattcat gccctggcag tatgtcacgt tctcgcgttt ctgaacgggg aacggcgctc
    11341 cattgaggaa gttattctga tgaaaaatat aaaattcagc ctggcctggc agattctgtt
    11401 tgctatggtg ctgggcattc tcctgggaag ctacctgcac taccatagcg acagccgcga
    11461 ctggctggtc gtcaatttgc tctctccggc gggtgatatc ttcatccatc tgatcaaaat
    11521 gattgttgtg ccgattgtga tctccacgct ggtggtgggt atcgcgggtg ttggtgatgc
    11581 caaacagctc gggcgtattg gcgcgaaaac cattatttac ttcgaggtga tcaccaccgt
    11641 cgccatcatt ttggggatca ctctggcgaa cgtcttccag cccggtgccg gggtggatat
    11701 gtcgcagctg gcgaccgtcg atatctcgaa atatcagagc actacggaag cggtacaaag
    11761 cagttcccac ggcattatgg gcacgatttt gtcgctggtg ccgacgaaca ttgtggcgtc
    11821 gatggcgaaa ggcgaaatgc tgccgatcat ctttttctcg gtgctgtttg gtctggggct
    11881 ttcttccctg cccgcgacgc atcgtgaacc gctggtgacc gtgttccgct ccatctctga
    11941 aaccatgttt aaagtgactc acatggtgat gcgttatgca ccggtgggtg tgtttgcgct
    12001 gattgcggtg acggtggcta actttggttt ctcttctctg tggccactgg cgaaactggt
    12061 gctgctggtg catttcgcca ttctgttctt cgcgctggta gtgctgggaa ttgtggcgcg
    12121 cctgtgcggg ttaagcgtct ggatcctgat tcgtattctg aaagatgagc tgattctggc
    12181 gtactccact gccagctctg aaagcgtact gccgcgaatt attgagaaga tggaagccta
    12241 cggagcaccg gcgtcgatca ccagcttcgt ggtgccgacc ggttactctt ttaaccttga
    12301 tggttcgacg ctgtatcaaa gtattgccgc tattttcatc gctcagctgt atggcattga
    12361 cctgtccatc tggcaggaaa tcattctggt gctgacgctg atggtgacct cgaaagggat
    12421 tgctggcgtg ccgggcgtgt cgtttgtggt gctgctggca acgctgggta gcgtaggtat
    12481 tccgctggaa ggtctggcgt ttattgctgg tgttgaccgt atcctcgaca tggcgcgtac
    12541 tgcgctgaac gtggtgggta atgcgctggc ggtgctggtg attgccaagt gggaacacaa
    12601 atttgaccgc aaaaaggcgc tggcttatga gcgtgaagtg ctgggcaaat ttgataaaac
    12661 tgcggatcaa taattgaaga ttgccgggga tatccacccg gcaatgtgtg aatgcctgat
    12721 gcgacgcttg ccgcgtctta tcaggcctac cttaaccgtt ggttaatttt tcaaactctt
    12781 cacacccggt atcaaaccct tccatacagc tcaggttcaa ccagtgcagc gccttttgct
    12841 tgttcttctc gataaaacct tcttcaccgt ttaagaacat cattcccgcc cagtactcgg
    12901 aataaccggt tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc ttgtcatcgt
    12961 cggccttaat gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg gcatcgacgg
    13021 cagagtcgtt ctccagatct tccgaggcgt tttctaacag cgaaatagcc tttggataat
    13081 ccggtttacc cgcttgagta tttaccagaa tatgcgccag cgttacttca ccttctttac
    13141 tgcccgcttg cgccgctttc tctgcaagaa cttttgcttg tggatagtcc agactgaccg
    13201 gattggtgat tttgatctgc gccagcagcg cgcaggcatc agcatcaccg ctgtcggcgg
    13261 ctttctgtgc ccaatattcg gctttgctca aatcgccgga gctaaaccag ctgtcggcaa
    13321 gaaaatattg tgcgcgtcgg tccccggcct ctgctgcctt taaatactgg ctgccaggct
    13381 cggagtcgtt ggcgtgggca aagaatgtca aaaacaacat caatgcgata atttttttca
    13441 ttttgataat acgattgggt agggagtaac cagtataaag agttcgaatg gataaaaaaa
    13501 cagcctccga aaggaggctg tagaaaggac tgtattacgc cagtgccgct tcgcgcaggc
    13561 gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc tgatcggcga
    13621 tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc gggcttaagt
    13681 tttcggtaac cagctcgtta caggcaccaa tccaccactg gtaggtcatg taaatcgccc
    13741 ctttgttcgg acgatcgctg acctgcgcac gggtgataat tttgccttta cgcgagtgca
    13801 cccaaaccaa tgcctcatct tcaataccca gacgtttggc gtcttcggta ttgatttgtg
    13861 cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc atcgaacggc
    13921 aagagtagtg accaacttca cgcaccgttg acagtaccat cgggtactcg tcggtgagtt
    13981 tgtcgattgg cgctacccag tcgcaggtga agaactgcgc cagaccgttc ggggtatcaa
    14041 acttctcttt aaacagataa gaagtcccct gatcggcatc tgaagtatcg cggcaaggcc
    14101 actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa tccgggcaca
    14161 gatgacgcaa ctcatcccag atctcctggg tgttgttgta gtgcatcgga taacccatac
    14221 gggtggcgat ttcactgatg atttgccagt ccgttttcag atcccatttc ggttcaactg
    14281 ctttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca tgctcgcccc
    14341 acgacgttga cggcaaaatg acatccgccg ccgacgcggt tttggtcata aagatgtcct
    14401 gaacgataac cagttccaga tcttcaaagg ctttacgtac tgccgacagc tccgcgtcag
    14461 tttgcagcgg atcttcgccc ataatgtacg cggcacgcac ttcgccatgc gctgcgcggt
    14521 gcggcagctc gctgatgcga taaccggtat gagcaggcag gctttccacg ccccaggctt
    14581 tggcgaattt ctcgcggtta gccggatctt tcacgtactg gtatcccgga tacgtatccg
    14641 gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc gggtttacac
    14701 ccgcatgcgg cttaccgagg ttaccagtca gcatcgcgag gctggtcaga gaacgcacgg
    14761 tttccacgcc ctggtagaac tgggttacac ccatgcccca caggatggcg gcgcttttcg
    14821 cctgggcata catccgtgcc gcctgacgaa tctcactggc gctgacgccg gtgatatctt
    14881 caaccgactc cggcgtgtag ccttcaacga ttttacgata ctcttcaaag ccttctgtac
    14941 gggaagcgac gaacgctttg tcgtacagat tttcttcaat aatgacatgg cccatcgcat
    15001 tcaacagcgc gatgttcgag ccgtttttca gtgcaatgtg catgtcagca atgcgcgcgg
    15061 tttcaatttt gcgcggatcg cagacgataa ttttcgcccc gttacgttta gcgttaatta
    15121 cgtgattcgc cacgattggg tgggaatcag ccgggttgta cccgaaaacg aacactaaat
    15181 cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc gattggtgca
    15241 gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta ttggtaccaa
    15301 taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta ccacgcgagg
    15361 agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc aggcgctcgg
    15421 taacgtaatt cagtgcctca tcccaggaaa caggttcgag tttgccgcca cgctggcgac
    15481 ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg aaatcccagc
    15541 cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct gcctccgccc
    15601 ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg caataggggc
    15661 aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg catacgcgct
    15721 ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc ctgatattac
    15781 gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt gacgtgacga
    15841 aacgaaatac cgcgtgacag ccatcacgcg gcagacattt tattttttct cgacgacggg
    15901 agccgcctga tacccgccgc ccagcgattt catcaactga atgctttgga tcacccggcg
    15961 gctgtccagc atcagtaatg acatctcttc ggcaagtact ggcaaccggg cttcagtggc
    16021 ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct gggtgaagcg
    16081 cgttgcttcc acgcgttcag cctgcatttc tcgctcgtcg ttgagcgttt gcagacgcgt
    16141 gccgttgacg gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac gttcaatcat
    16201 catgttgctg gcggcgcgcg tgccttcgag attggcattc aaccgtccac cgtcaaacag
    16261 cggcaatttc agacccggga tgaagttgaa ctggcgactg gtttttttga ataaggtatc
    16321 caggtggatg gcatcaaggc cgaaaaacgc tttgatatca aagctcgggt agaacagcgc
    16381 ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagcgcatgg cttgcagatc
    16441 cgggcgtctg gcgagcaact cataagagag tgtcgccgga atgccggtct ggactcgtgg
    16501 taatgccacc ggtttgatct ccggcatatc gctggccccc gcgccaatca atgcacgcag
    16561 agattctcgc gtttcggtga tttgcccttt gacggcagca atttgtttat cgaccgccag
    16621 aatctgtgcc cgcgcgccgt ggaaaggcac ttgtgcttcc agaccgtgcg ccactttact
    16681 ctggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca gatcgagcat
    16741 ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca gcgatagctc
    16801 tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg caaccgctga
    16861 gcgatgcaca ccccacaaat caagatccag tccggcaaac aaacctactg tggcttccgt
    16921 atagtacggc ccgtccatac ccagtgcggg cgcatccatc gcataagggc ttaaaaagcc
    16981 gttcgccgag acgcgttggc ggttgagcat ccctaacgct gcgacctgta attgtgaacc
    17041 ggcatctaac aaatcggcct gggactgcgc tttttcttcc cgcagtttcg cttcggcgag
    17101 ggtgtgtgaa ccacttagcg tccgttggat cagcgcatcc agctgcgggt cattgagttg
    17161 tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcat cggccagttt
    17221 gatttgttcc ggtttgagct gttgatgagg cgcagaatcc ttgcgtacca aggcacagcc
    17281 ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa gttgacgatt
    17341 gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg ggctgtccag
    17401 tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa ggtgggcagg
    17461 atggcctgcg aggcgggtgt ctcttcaagc gttatttgag gagaacggct gagtgcggtt
    17521 gccagaccgg cagcgtattt ttccagggcg tcggcaaact gacctgctct gtccggctgt
    17581 agtgcgttat ccagcgcctg cgccgaattc caggtggcat cccaggcgtg aaggatatcg
    17641 cggccctgac gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc ttcgctgtcc
    17701 agttgacgct ccagcgccac gcgttggcac atctcttcac aggcattaaa cgccgcatgc
    17761 agaccgatac gaatttgcag ataagtgcgc agagccgtga cttcctgctg gcgtgggatc
    17821 cgcattactt tacttaacat acccagcgcg ccagccagtt tttgcggcag cgtgcgcgct
    17881 tcactttcag gccagacaaa ggtgtaaatc accgcggaca ccacggtacc aatgatgata
    17941 cccagggcgc gatcgcgaat ttccaccagg tcgtacactg ggccaaaaac gttttcgagc
    18001 gtggcgagcg cgaaggtgac caccatctgt gtgccgatat aagaagagcg ttcagagctg
    18061 gtggcaatcc atgcgcccaa caggaaaatc ggtgccagca caaacagcaa ttcgacaata
    18121 ttgtccagcc agggcatgac cagtagcgtg aataacagcg ccagaatcgc gccgcaaaag
    18181 gccccgccaa aacgcagcac catcttctgg tacgacgaac cgacatttgg attagagacg
    18241 atcacgcagg tcagcataca ggtgtgaatg ccttcccagt ccacgccgct gtaaaaggtg
    18301 taacagatca aacaggcgag caatgttttt accgcgtagc gtatatagtc tggattggta
    18361 aaagcatcgg cgaccattga tggcggtttg gcggcgggcg ttggcggcgt attcgggttc
    18421 atctggccca gttgtaacaa cgtctggcag atattctcca ggttacattc ccgtgccgcc
    18481 accgcttcac tttcactgat ccgccagtcg ctttgccagc actgaccttc agcaacggca
    18541 tgctgcagct tgttgatttc tgaggctaat ttttgccgaa attcaataat tgcctgagaa
    18601 tcagcaaaag aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt taccgttgcc
    18661 acgcagcttt gccaccatgc gctttgagtt cgccagttgg catcgtccgc gagacaaaag
    18721 acattcagtt tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg tagcggtgcg
    18781 aggctgtctg tcaggtgact aatggcatca tcaagccgat cattaagcgc ctgatgcatt
    18841 tgcgaaatgg cacgactggg aaaccacagc acgccgatta acgtcatcag caaggttgga
    18901 tagaggccaa caacgataca ccacagcgtt aagcgcacga ccacttccgg atagtcgagc
    18961 atggcgggga aggtttgccc gtaaatggcg acaatggcga cagcgaaaaa gaccagtccc
    19021 agccgatggg tgcgcatcaa aaacatgcgg cccatcagga tcggcccggc gatgatcaat
    19081 cggatcaacg gttcgccgta tgaccatttg tagatcaaaa acaggctgcc gatctccagc
    19141 accgtggcaa ccacaaacaa gatcgccaca aatttggtgt aaaacgcgtt cgactgaata
    19201 ccgtaaaaca gcactgccag cgataacgcc acaaaaggga tctcaaaagt catcgagatc
    19261 agaatcacca gcaggcagcc tacccagagt tgcacgatct gcggcacgcg gccgggtcgc
    19321 cgctcgctta actcttcatg aaagaacgcc agcagcctga ccatcggtaa tggcagggag
    19381 ttgagcgcgc tcattattgc ggctcaagat tagcgactgc cgaagcgccg atgcggaaca
    19441 tttccgggtc aggtttatcg accatgattt tgaccggaaa acgctgggca acgcggaccc
    19501 agttaataga gcgagaaact ttcggcaggc cgcccagcac caggccgccg tcatccggta
    19561 gcacgccgta gccaatcgaa tccactttac cctcgaaggt tttgccgctg tcactcatca
    19621 ggcgaatcgt tgcgggtgta cctgagcgaa tatttttcag atcggtttcg cggaagttgg
    19681 cgatcacgta ccagtgacga gtgtcgatta gggtaaaaat agggcgcatg gcagaagcaa
    19741 attgcccgac ggaggttttg agggaaatga cccggccatc aaacggcgcg cgaacggtcg
    19801 ccatttccag atgcagtttg gtcagggcaa tatccgcttc gaccgccgca cgttgcgcca
    19861 ccagcgcatc gacgccgctg acggcgctgg cggctgactg cgcctgtaac aatacggcat
    19921 taagatcggc ttctgcggcg cgctgcgcag ttcttgcacg gtcaacgtct tccgctgaga
    19981 caaaaccctc tttcagtaat ggctcggtac ggcgtaatgt atctgtggcc tgtttcgcgg
    20041 cggcacgggc tttttctacc gtggcattaa tcgagtcggc accaaactgt tgcgcatcaa
    20101 cgctacgctg ggtgagcata atttgcttat ccagcgccgc gagggaggct tcagctttcg
    20161 ccagattggc ttcgtacggg cgcgggtcaa tgcggaacag caaatcgccc tgtttgactg
    20221 cctggttgtc ggtgaccgcc agttctacaa tgcggccgct gacttccggc accacatcaa
    20281 tggtatctgc tgacgcgtaa gcgtcattag ttgatggcgc actgtctacg cgccagataa
    20341 cgaaaacaag ggcaaccagc gccaatgcaa ccactaacag agcagggaat ttactgcgag
    20401 gagctttttt cggcgtactt tccatgagcg cgtctcaaat agattagaaa aatgccagcc
    20461 agaacagcat ggcgtagaga gcaaaaaggg cggtataaat aattccgaca aatgccagat
    20521 tgatatttgc ccgctgaata actcgcctcg tgatgagcat caaaataagg ctggcaatgg
    20581 cgcagaaaaa ccagctggga taataagcac cgatcaccgg gattgccgga gataatgagc
    20641 atcccgaaac catgaagacg ggaattatcc aggctgtttt tttgagttgc atcgccatgc
    20701 gagagagaac tgttggcata ttaaatacat cctgtctgct gcgaacaata tctgtagtga
    20761 atatcaatat ccattgaagg cgtcagtatg gctattttct cgatgaataa aaatagcttt
    20821 attcagtcac cgcattcata acttatatat tttatttatt aattggctgt atatattttt
    20881 aaaataaatg cttttggggg cttcagcgcc cccaaaagcg agtgacatta atttggggtt
    20941 acgatattca cccacaaatc gaaattatcc agacaggcga taatttcttc cagtttcgcg
    21001 ccattgccaa taattttggc tttcttcgct tttaccagat ccgccatttt ggcttgcccg
    21061 gtcagcacgg cgtgcagatc ttcacggctg atgtagaaag aggcgtcagc ttgggattgc
    21121 agtgttttac ggtagttaag cacgctatcg ttcagcgtca ggttgaggtt atcgccattg
    21181 ctcatattga agttcaggct gatattttta cccgcggctt tcgcgctatc gaggcgaacg
    21241 gacataaagt cgaacagcat ttcgaccgac atcccgcgaa tggtgtccgg ggaaccgatg
    21301 gtgccgtggc tgaacttatg caccccttcg cgcagctctt tcgcgccggt caggtagaaa
    21361 ccgcgccagg tggcggactc ggcctgatag cccagctgtt caaagttatt cgcttgcaga
    21421 ttcttcgcga cctggtcacc cgggttggcg gcaatcacct gtttcagcag ttctgccgac
    21481 cagcggtaat caccttgctt gttcgcttct tgcgccaggt tgatgacacg ggcagaaccg
    21541 cccagcgcct gcacgtaacg tttacccatc tccacctgac catacggatg caggttagcc
    21601 gggttaccgt cgtaatagcc aagatagaag ttatacaccg cgcgggcgtt gtggctgaca
    21661 gaaccgtaat agccacggct ggcccagtta ttggcaagtg caggcggcag tttaatcatg
    21721 tcgccgattt cattcatggt gtagccctgg ttcgccaggt gcagggtctg gtcgtgaatg
    21781 tacttgatag tatcgcgata tttaccaata taatcattga tatgcttatt gccccagacc
    21841 ggccaggtgt gcggcataaa caggacttcc gcgtcgttac cccacatatc cagcgtttcg
    21901 ttcagatact cggtccactt gctggtgtcg cgggttttcg cgccgcgcag agtgtagaag
    21961 ttgtgcaggg tatgcgtggc gttctcggcg gtacacagcg ctttcagggc cggaatatag
    22021 aagtgcattt cggctggtgc ttcgctacct ggggtcatca ggaagtcaaa ctccaggccg
    22081 tcgataatca tcttctcacc ggttttggtg atggttttgt tcggtgcgat gatgctcggg
    22141 tagcccgatg ccagcgttac gccaagacca ttgccgatgt taccctgagg gttatgcgcc
    22201 agcagcaggc cgtaggagta aagtgcacgg cggctcataa tattacccgc cagtacgttt
    22261 tcgctgatgg cttcgtccat aaagccagca ggggcgataa cctgcacttt accggatttc
    22321 acatcagctt cagagataat gcctttcacg ccgccatagt ggtcggtatg gctgtgggta
    22381 taaataaccg caacaatcgg tttttgcggg cgattttgga agtaaaggtc aagggcggct
    22441 tttgcgctcg gtggcgtcac cagcgggtcg atgacaataa tcccggtatc accttcgata
    22501 aaggtgatgt tggagatatc ctgaccgcga acctggtaca ttctgtcggt gactttaaac
    22561 aggccggaaa taccgtttaa ctgcgactga cgccacaggc tagggttaac ggtttcgggc
    22621 gcgggcgcat tgatatcaaa tttataatct tccccgcgat aatatggttt gccattagca
    22681 tcgcgcagaa taccttcatc cagcagcgga gcgataaatc cacgctgagc gtcgtcaaaa
    22741 tcctgacgat cgctaaacgg taatgatttg gcgtaattct gattaacctg ttgggtatat
    22801 tgagtggcgg ctttcgcttc ctctttcgcg ttagcggtat ttaccatcat gccgctggcg
    22861 gcagttaacg caataacaat tctgctcaaa cggaataacc gagagttatt catgtaaatc
    22921 tccatttatc atttatgtcg taaatatgta atccatgcaa aatacgtcat ttaaagctgg
    22981 ggttatcata attgggaatt tgcgacttta ataagtggaa gtgtgagctg aacgatccat
    23041 tttattaggc atttgtgctg aagagggaaa attacgcgaa tttattatct ctgtaaataa
    23101 catgaggtgt tttatatatt tattatatat attgtacttc ggcaaatact gagcacaatt
    23161 tatcaatgca accggtgttt ctttttaaaa aacgtaccgg agcaatgggc ggtcatgtcg
    23221 ctggcaccgc aggattctgc gttgctgtgc cagcctttta ttcatggtca atgagtgagg
    23281 tgattgataa agacaccagg tcgtcagagc gcactgatta tccgcattat gccagtgaag
    23341 ttgcactggc ttttggttca cgagtggttg gcctcgaact ggcaaaaatg attgtggatg
    23401 cgtggctggg cgcacagtac gaaggcggtc gtcatcaaca acgcgtggag gcgattacgg
    23461 caatagagca gcggagaaat tgagattcat ccactacttg catggatgag taatgactaa
    23521 tgtggataga gtttcttttt gaggttggct aatgaaacgc tttccgctat ttcttttatt
    23581 caccctgctc acgctgtcca ccgttccggc ccaggctgat attatcgacg atactattgg
    23641 taatattcag caggcgatta atgacgctta taaccctgac cgtgggcgag actatgaaga
    23701 ttcgcgcgac gacggctggc agcgtgaagt gagcgacgat cggcgcagac aatatgacga
    23761 ccgccgccgc cagtttgaag atcgccgcag gcagctggac gatcgccagc gccagcttga
    23821 tcaggagcgt cggcaactgg aggatgaaga gcggagaatg gaagatgagt atgggcgatg
    23881 aggttgggta tggagcaggc atcgcccctc accccaatgg ggagagggag aaaacgagcg
    23941 caatattcaa tattgagcac aatcggtccc ctcgcccctc tggggagagg gttagggtga
    24001 gggggccagc gcgcacattc cctctcatca cgccacccca atctccatcc catcaaaccc
    24061 cacctcaaac cctgacggta gtgcgttttc catcagccac gcatcaaact ggtggctgat
    24121 atgggtcaga atcacccgtg gcgagcggat aacctgattc agcgcaagca cggtatttaa
    24181 atcacagtga ttacgcggtg catccgcgcg cggcgggtga ctgcaatcca tcaccattac
    24241 ctgcggctga ttattgcgta aaaatttcag cgttttttcc ggcaagccag cggtgtcaga
    24301 cagccacgcc acccggctgt gcgccgtttc cagtaaatag ccgaaagtga gttttgagtg
    24361 gttaagcggc agcggcgtga cctgcaaccc ctgcaaatca aacaccacaa acggttccac
    24421 cgtgtggctg aaatcaagca gcccgggatg tttaaacaga tcgtcgcagc cctgttcatc
    24481 cggcgggccg tagatcggga tcggatcgcc cacgccccag cgcagcggaa acagcccctg
    24541 gacgtgatcc atatgataat gcgtcagcaa aaactgctgg aacgatccgg gagaccagcg
    24601 atcggtgaga tcgtgcagcc cggcgtcgat cagggtgatc gc
//
LOCUS       DAJTVJ010000059        24619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000059 DAJTVJ010000000
VERSION     DAJTVJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.059"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..186)
                     /locus_tag="OO448_002283"
     CDS             complement(<1..186)
                     /locus_tag="OO448_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337477.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRN"
     gene            complement(183..605)
                     /locus_tag="OO448_002284"
     CDS             complement(183..605)
                     /locus_tag="OO448_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCR6337478.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRVSRQVTVPAVMLEKVVALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(652..1077)
                     /locus_tag="OO448_002285"
     CDS             complement(652..1077)
                     /locus_tag="OO448_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6337479.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHIVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(1495..2265)
                     /locus_tag="OO448_002286"
     CDS             complement(1495..2265)
                     /locus_tag="OO448_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337480.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSCGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            complement(2316..2750)
                     /locus_tag="OO448_002287"
     CDS             complement(2316..2750)
                     /locus_tag="OO448_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCR6337481.1"
                     /translation="MYGTCEILCRELAAKYPADTPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSGVAMEIISNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
     gene            complement(2762..2983)
                     /locus_tag="OO448_002288"
     CDS             complement(2762..2983)
                     /locus_tag="OO448_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337482.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2984..3667)
                     /locus_tag="OO448_002289"
     CDS             complement(2984..3667)
                     /locus_tag="OO448_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6337483.1"
                     /translation="MSRFVLGNCIDVMARIPDNGIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYVLAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3744..4055)
                     /locus_tag="OO448_002290"
     CDS             complement(3744..4055)
                     /locus_tag="OO448_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337484.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(4052..4954)
                     /locus_tag="OO448_002291"
     CDS             complement(4052..4954)
                     /locus_tag="OO448_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6337485.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNCIQVDF
                     DTPWCQPESDVVAALSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            5372..5620
                     /locus_tag="OO448_002292"
     CDS             5372..5620
                     /locus_tag="OO448_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6337486.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            5617..6054
                     /gene="umuD"
                     /locus_tag="OO448_002293"
     CDS             5617..6054
                     /gene="umuD"
                     /locus_tag="OO448_002293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6337487.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            6054..7328
                     /locus_tag="OO448_002294"
     CDS             6054..7328
                     /locus_tag="OO448_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR6337488.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGHRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(7330..7746)
                     /locus_tag="OO448_002295"
     CDS             complement(7330..7746)
                     /locus_tag="OO448_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6337489.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(7739..8719)
                     /locus_tag="OO448_002296"
     CDS             complement(7739..8719)
                     /locus_tag="OO448_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6337490.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            complement(9133..9441)
                     /locus_tag="OO448_002297"
     CDS             complement(9133..9441)
                     /locus_tag="OO448_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HCR6337491.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            complement(9528..10172)
                     /locus_tag="OO448_002298"
     CDS             complement(9528..10172)
                     /locus_tag="OO448_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR6337492.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            complement(10352..11131)
                     /locus_tag="OO448_002299"
     CDS             complement(10352..11131)
                     /locus_tag="OO448_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCR6337493.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            complement(11133..11546)
                     /locus_tag="OO448_002300"
     CDS             complement(11133..11546)
                     /locus_tag="OO448_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337494.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            12078..13004
                     /locus_tag="OO448_002301"
     CDS             12078..13004
                     /locus_tag="OO448_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337495.1"
                     /translation="MSEKEHDMTNDGGESVTYTLRNIPADTDRVITDLASHARKPKAT
                     FLREFLEDSFRDVIDSFALKNPLIASLDEELASYLDAKVMEQRYQSHFITRWNQEYQK
                     LLGISTEEELRRLVLNNTPFLQVRADQVLKGWKNIPRGISLTFSLFAEIAGRDRETID
                     QAWKNIFYSQLREKKHRFYQDIEAIRALKKLPALTGDSWTRDGITVRIYRPENYARGA
                     WRVTLSLPENYATQMWNIPFPELEYRLFTADPGYSALISAEPDRWDKAFRFVDGVCEL
                     HLYTNGVEEDHNPTPLGDVAQALINVVEENLL"
     gene            13049..13303
                     /locus_tag="OO448_002302"
     CDS             13049..13303
                     /locus_tag="OO448_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337496.1"
                     /translation="MKGRQSRYVTGGESFAEIARLPSGAVVRLCLNTGLEDALREASK
                     SLKSAFTRSGRKCRLSAGTAQGPFTGRRQGVATHLFVSVL"
     gene            13802..14146
                     /locus_tag="OO448_002303"
     CDS             13802..14146
                     /locus_tag="OO448_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HCR6337497.1"
                     /translation="MKNLLDTEGTVLFPFQTEIHFIWTIFSTVKRLVIGTRDHICQKQ
                     YWSACLCILLLMAYVGLCAAVVWFVVPWLKAFIVSKFAVSALWVTPVKRGLLWGIGKV
                     VTATCRRFTLLK"
     gene            complement(14322..15323)
                     /locus_tag="OO448_002304"
     CDS             complement(14322..15323)
                     /locus_tag="OO448_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HCR6337498.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETRPERPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
     gene            15675..15929
                     /locus_tag="OO448_002305"
     CDS             15675..15929
                     /locus_tag="OO448_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337499.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRECGLDNQSARCISELKASALL
                     WLWYELAIHGAPQNNHAQARERIDTDYQRLKKLIWSEGDS"
     gene            15929..16519
                     /locus_tag="OO448_002306"
     CDS             15929..16519
                     /locus_tag="OO448_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337500.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVCLEGDPLEVAQWLQACHDAGIEVRVQINESSVP"
     gene            complement(16537..16884)
                     /locus_tag="OO448_002307"
     CDS             complement(16537..16884)
                     /locus_tag="OO448_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337501.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVAVLTGGLWGTSWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            17003..17350
                     /locus_tag="OO448_002308"
     CDS             17003..17350
                     /locus_tag="OO448_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B immunity protein"
                     /protein_id="HCR6337502.1"
                     /translation="MKLDISVKYLLKSLIPILIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFILCKALSGK"
     gene            complement(17368..19248)
                     /locus_tag="OO448_002309"
     CDS             complement(17368..19248)
                     /locus_tag="OO448_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B"
                     /protein_id="HCR6337503.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AINSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     NIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWAFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(19527..20873)
                     /locus_tag="OO448_002310"
     CDS             complement(19527..20873)
                     /locus_tag="OO448_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337504.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            21227..21829
                     /locus_tag="OO448_002311"
     CDS             21227..21829
                     /locus_tag="OO448_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCR6337505.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            21885..22379
                     /locus_tag="OO448_002312"
     CDS             21885..22379
                     /locus_tag="OO448_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337506.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            22379..22645
                     /locus_tag="OO448_002313"
     CDS             22379..22645
                     /locus_tag="OO448_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337507.1"
                     /translation="MLTGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(23122..23595)
                     /locus_tag="OO448_002314"
     CDS             complement(23122..23595)
                     /locus_tag="OO448_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337508.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
     gene            complement(23712..24461)
                     /locus_tag="OO448_002315"
                     /pseudo
     CDS             complement(23712..24461)
                     /locus_tag="OO448_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
BASE COUNT         6292 a         6411 c         5995 g         5921 t
ORIGIN      
        1 gtttctgctg ccctgaagga tatagtgacc gcccaggaaa accgcctgct gattcaccac
       61 aaaatcgcag atttcatcaa caatctgcgc agtggtgagc ggtttctgcc ccatctcacg
      121 acgccgcgca ttaatcacgg ctttgtaatt gcgcagaatt tcgcgggttt ctgtgctgat
      181 attcatgaca tgtgctcccc gtccagcgcc tgtaacacaa cgtccatcgc ttcgcgttct
      241 tccctgacga atgcgtcacc gtcgcccccg ttctcactcc cgacggcata caggcgcttc
      301 atttcactgc ctgccagcgc caccactttt tccagcatca ccgccgggac tgtcacctga
      361 cggctgacgc gcttttcttc catcagttca cgaacaacgt cgtgaacaac gctgacatcc
      421 gcaccatgct caaaggcatc gtccagccgc tgcatgacgg tttccagctc atcgtctgac
      481 aggttccagt cctgggctat gtgacgcaca tccccacggg tcagcaccac ggcaaaaacg
      541 cattcactgt ccggcacgtc tgtatccagc acttcgcgga taatttcttt aacagtacag
      601 tacattttct cgtctccttt acaggggcgg cattgccgcc ccgtccgtta atcagtccag
      661 agccgcgtaa atcgcgttgc attcagggtg aaattcgatg tgacgccaca actgcgcatc
      721 gcgcatccgg taaagctggg tcagtcctgc atcaccgctg tcgtggtaca gccacaactg
      781 gcggttaatc acaagggaag tcagaatgat gccgcaggca tcagcactca cggtacggtc
      841 aaaccagttt gcaccgttca ctatgtggaa acggtcgcca tccggcatca tgtagccgcc
      901 accgtccggc aggcggatga agtgccagaa tccgccttca tattccggca tcattttttt
      961 cgccagcgcg tacacagacg cttcgacata cagaaaatcc tggccaaaca ggtaaggcag
     1021 aaaaaacaga cggtcgcact cttcaacatt cagagtgaca ggtttcgcat attgcatgat
     1081 gtattcctcc gttgttcaca tattccgcga caccgggcgg tgccgtgcct ccataaaagc
     1141 cgggcgataa ggcggggatg cacagggcgc aagggaagcc gcagcctgac gcacacggct
     1201 ggcgggagtt ggagggtgag accgcgaaac ctgagcgggt gaacgaccgg aaaatcacgg
     1261 cagacgggaa ggagggaccg ggtgaccgca gcggcgaagc gtacccttgc atccctgcca
     1321 gcccggctaa gatggaggca cggcacagcc cgcacacgac gctgaggcca cagagctgaa
     1381 aacccacaag gccatgcccc gcatggccgg gtaaagcgga gccggcggcg acttcctttg
     1441 tgctctgtcc ggggaccggc aggaaaaagg cggggattta ccccgccagt gtcatcattt
     1501 ccggattttt gccagggcat ccgccatgct gcggatttcg gtccgggctt cccggaccac
     1561 ctcccgcaga acaccacggt caaaccagcg gcggggcggt tcatcgggta tacgcagcac
     1621 atcgcaggcc agcccacagg acaggatatg ctcaatacaa tcgctgacca ggtacatttc
     1681 caccgacgaa aaacgtgctc cccggttcac gatggcatcg agcaccacgg gcaggttttc
     1741 gtcatcttca tcgagccagc aggggtgatc gctttccttc acgaccaggc gaaagcggcg
     1801 cgtctggcgg gtcagcaacg tctgaccggt ggtggtcagc gccgcacgga tacgccgcgc
     1861 atcttctgcc acctggtaac agatgcttcg taccgcatcg tgcaccgcat caagaacagc
     1921 cctgatttcg tcttcggtca gccattctcc ggcatacggg aaagacacct gcgttccgtc
     1981 tgccggaagt accggcagcg actgccactg actgccggac agtaccagtt ccgccccggt
     2041 tccggtcagt ctctgaatgg cggaggccag gagcggcggc agtacgccgc cgtgacggtt
     2101 gataagtaac gtcgccagcc ggggcgcatc cagccgcacg taaatgctga tccagcccgg
     2161 atagttcgga tttcgctggt caacatccgt ctcttccggc cagccctgcg ccagcagcag
     2221 gttacgggcg tgacgacaaa ccagtgcatt cagcgttttg ttcatgatta agcgttctcc
     2281 ctgtatcgcc gcccgcaggg caggcggcga taacatcagt tgttttcgtg tgtgttgttt
     2341 ttcagcaggg tgcgggcctg attaatcact gcctgacggc gggtaacaca ttccgggacg
     2401 gcaaggccgt tatcacgggc ggcccattca cgtttccaca gtgcctgttc agcggtctga
     2461 atcagggacg ccagcagttc agcagggacg gtcacctggc ggttttctct cacgttgctg
     2521 ataatctcca tcgccacgcc ggaagaaata ccggactcaa tccgctggtc ttccgggatg
     2581 tcctccaggc gcgccaggac ggtcctgatt tcatgatcgg acagggaaat atccattccg
     2641 tcagcgaggg cctgaatctc ttcaggggac cagacaacca gcatcagtgg cgtgtctgcc
     2701 ggatactttg ctgccagctc ccggcagagt atttcgcatg ttccgtacat actctctccc
     2761 ctcaatcttt gaagcaggaa tccagttcca gaatttcggt atatgcctcc atcaggaggc
     2821 ggtttgcgcg aaacaacgtc tgacgcacca gacgttccgg cagggtgtca gcattaaagc
     2881 tgtaccgccg ctccatctca ttcatggttg tacgcaggtc gcacatggca ttggccacct
     2941 ccgccacttc ggcgcgaagt ttttcatgtc cggcatagtt catttacgca gcctccggca
     3001 taaaccagtc atcatttgcg gccccctgct gcatggcccg ctgtacggca gccagacgtt
     3061 gctgtccggc acggtgatac tgctcaagca gctcgatacc gatataccgg cgtccggact
     3121 gaagggcagc cacgcaggtt gagccgctgc ctgcaaacgg gtccagcaca atcgcgttcg
     3181 gatgtgtgaa gctctcaatc agtggttgca ggctggtaac aggcttttcc gtcgggtgat
     3241 gacgattgcc tgaatatttc cagcccagca cgtcgggtaa tggcttctga ggcagcgccg
     3301 gacggccttt tgccagaacg taggcgcatt cgtggcgata acccacatat gcggccttcg
     3361 atgtgtagtt tttggtgaag accaggtgcc caaccacact gaatcccgca tttttccagg
     3421 cggccataaa gcgatcgacg cggttccagc cgtagaagct gaccatcagc gcatcttttt
     3481 tgagtacgcg atacatttca ttacaggctg gttgcagcca ttcatcggtt ttatcgccgg
     3541 cgatggtacg cccctgtcgg tcacgaaaac cgacgaggta tggcgggtca gtcagtatga
     3601 aatcaatgcc gttatcagga atacgcgcca taacatcaat gcagttaccg aggacaaaac
     3661 gggacataac aacactcctt ctttctcaga gggcaccggg agggcacccc tgtggtgccc
     3721 gttccccgct gcctgtgggc tgttcaccgc gtccggtaac gggtcaggcc gaccgtggaa
     3781 tatcgcagcc gtcgtctttt tgacggcgag aatatgccgc gaaagcggct tgaagggcgt
     3841 tcatcagaaa agggcgacag gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     3901 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3961 gccgcatcag ccggcgcagt aaacagagga gcacggcgac atcctttgtc ggaaaagaaa
     4021 cgggcggttg cccgcccgtc tccgtcatac ttcatccccc gtaatgcgcc accttgtcga
     4081 ctatccacgc aggtccggtg acttccggca gctcatcgtc atctgtcggg gaagaccatt
     4141 ccagttcccc ccagagcaca tcaacgagct ctccgcgctc atacaactgc cagccacaga
     4201 aattgcagcc ctgttcggcg taccagtgct ccagcgtgca gccgaaacgg cggcttaatg
     4261 ctgcaacaac gtcgctttcc ggctgacacc acggcgtgtc gaaatccacc tgaatgcagt
     4321 tgtctccctg actactgata ttcaggtcat acccacaagg ccacttcacg ccataccgtt
     4381 ctgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccattca
     4441 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4501 gcactacgtt gtcacacaga cggttccacc acaccgatac atcctcgcta ccccagatgt
     4561 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg acatcatccg
     4621 gtaatccttc ccagcgacgc tgtccagtac cactctgcag ccagagttca tgcagcatca
     4681 ggcagttttg ctcatccagc agtacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4741 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4801 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4861 cttcatttgt ggcgcggcga taaagcggtg taactgcacc gctggccagt cgtttaatct
     4921 cagcgatctg tgccggttca ccagaaaaat acatacgatt cgagcaccag tttggcatgg
     4981 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgttac cgggcgaccg
     5041 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaacg acgaaacgtg
     5101 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     5161 ccggcacggc ctgggtaacc aggtattttg tccacagaac cgtgcacaaa atgctgtgga
     5221 taagctggac acagcagccc acagcaggca tacagccaca cactcagaga attgagcgtt
     5281 ttttagtcac aacaatgaca tttgactgtg tgcggattca gttattagaa tactgtatat
     5341 acatacagca aaagaaaagg gagatgagaa catgatccgc attgaaattc ttttcgaccg
     5401 ccagagcaca aaaaacctca aatcaggcac acttcaggcg ctacagaatg aaatcgaaca
     5461 acgtctgaaa ccacactacc cggaaatctg gctgcgtata gaccagggca gcgccccgtc
     5521 tgtatctgtc acaggagccc gcaacgacaa ggataaagaa cgtatcctgt ctctgctgga
     5581 agaaatctgg caggacgaca gctggctgcc tgcagcatga gtaccgttta tcaccgtccg
     5641 gctgaccctt caggcgatga cagttacgtg cgaccgttgt ttgccgatcg ttgccaggcc
     5701 ggttttcctt cgcctgccac tgattatgct gagcaggaac tggatctgaa cagctattgc
     5761 atcagcagac ctgcagccac cttctttctg cgcgccagcg gtgaatcgat gaaccaggct
     5821 ggcgtgcaga atggtgatct gctggtagtg gacagggccg aaaaaccaca acacggggac
     5881 atcgttatcg ctgagatcga cggtgagttc accgtcaaac gactgctgtt gcgcccacgc
     5941 ccggcactgg agccggtttc agacagcccg gagttccgca cactgtatcc ggaaaacatc
     6001 tgtatttttg gtgttgtcac tcacgtgata cacaggacgc gggagttacg ctgatgtttg
     6061 cactggctga tatcaacagt ttctacgcct catgtgaaaa agttttccgc ccggaccttc
     6121 gcaacgaacc ggtcatcgta ctcagcaata acgatggctg tgtgatcgcg cgcagcccgg
     6181 aggcaaaagc ccttggcatc agaatggggc agccctggtt tcaggtgaga caaatgcgcc
     6241 tggagaagaa aatacatgta ttttccagca attatgcgct gtaccacagc atgagccaac
     6301 gggttatggc tgttctggag tcgctttctc ccgcagttga gccctactca attgatgaaa
     6361 tgttcattga tttgcggggg ataaatcatt gcatctctcc ggagtttttt ggtcatcagc
     6421 tcagggaaca ggtaaagagc tggacaggac tcaccatggg ggtgggcatt gcgcctacaa
     6481 aaacgctggc taaaagtgca cagtgggcaa caaagcaatg gccacagttt tccggagtgg
     6541 tcgcgctgac ggcagaaaac cgtaatcgga tcttgaagct actggggctg cagccagttg
     6601 gtgaggtctg gggagtagga cacagactga cggaaaagct gaatgcgctg ggtattaaca
     6661 cagcactgca gctggcgcag gctaacacgg cattcatccg gaaaaacttc agcgtcattc
     6721 ttgagcgtac ggtacgcgaa ctcaacggcg agtcctgcat atccctggaa gaagcaccac
     6781 cggcaaaaca gcagattgtc tgtagtcgca gttttggtga acgaatcaca gacaaagatg
     6841 ccatgcacca ggctgttgtt cagtatgcag agcgggccgc agagaaacta cgtggggagc
     6901 gtcagtattg ccggcaggtg acgacatttg tacggacatc accctttgca gtaaaagaac
     6961 cctgttacag caatgccgct gtggaaaagc ttccattgcc cacacaggac agccgggaca
     7021 ttattgccgc cgcatgcaga gccttaaacc atgtctggcg tgaagggtac cgctatatga
     7081 aggcaggtgt catgctggct gatttcacac catcgggtat agcgcagccg ggattatttg
     7141 atgaaatcca gccccgtaaa aacagtgaaa agttaatgaa aacactcgat gaactgaacc
     7201 agtcgggaaa agggaaagtg tggtttgcgg ggcgaggaac cgcccctgaa tggcaaatga
     7261 aacgggaaat gttgagtcag tgttatacaa ctaaatggcg agatattccc ctggccaggc
     7321 tgggttagtt cagtcatctc cgaacatatt ttcagcgttt cttctggtct ggtcttcacc
     7381 gtcatttctc gacagactct gctctgtaag aggagtactg caatccacag acgtatggag
     7441 accagattgt ttttccagta attcaagcaa tttcttttca accgaaaaag cacctggtat
     7501 tacgctggaa atcaccttat taatatcatc cagagtcgta ttttctgtca gtaactccga
     7561 tatcagcaaa ggaagacgtt tatctatagc tgcaaacgcc gcaccagtaa ctaacatcgc
     7621 gcgcatgaaa cgtccccgct ccttcagttt ttcatttgca atgagctcat ctgctatctt
     7681 atctcctgtc cgttcatccg ggaaaagttc aaatgagcag cgtctggatt tattttcatc
     7741 actcattagt cacaccacct ttcattttta tcattcccat gactaaatca aactgcgggg
     7801 atgagctcag ataaaagcgc tcatcaggaa ccttagtcag gttcttcaca gcctcggcca
     7861 caatttcagc cccaccacca acacacatta catgtgtata gtcggtgaaa cggctgatga
     7921 catcattaac tcgtttaata agcaacttct gccgttcatt gataacatta atgatagact
     7981 cacgctgttc agcattataa atgcgacggg aaaggtagtc aggttcatta cggtgcacaa
     8041 tgatattgtc agcctggaat gagctcactc ttgtattagc atgtacagcc atctgttcct
     8101 taaccccgga tgtaatcagg gacacgccga tattcggatc acaccaggtt tttgtaatcc
     8161 ctgtcatttt gctacgaaca tgcgacacat caagagttgt accaccgaga tctacgatca
     8221 aaagggattc atcatcctca agccccgcaa gaacactgaa accagcggga atactttcag
     8281 gaagaacgga aacactgcga ataacaaatg catcactccc ctgcacccgt acttcacgca
     8341 tcacattttt tttctttctt tcaatgtttt gcttattttt ctgattgttg gcgtcaaggt
     8401 attcagagat cggcaatgtg acgatgacat caacaggctg cgctttcagg ccggtctgct
     8461 gtagcgcatg ctgaatggcc acaacattca catcactgta ctgatagcgt gtttcagtcg
     8521 ttacgacagc atcagcactt aatggatcaa atgaaaactt ctcgccgtcg atctcgtaat
     8581 tagccggagc agcatcatcc agtaaactaa aagaccattc cggtttaaag ctgttagggc
     8641 tgattaacgt tttcacgtca ccatcctcca accaggcaag tttaatgttg gtagaaccat
     8701 catcaataaa gattttcata accagtctcc gttgctcata aacaaataga aaaaacaaac
     8761 atacttcata catgcaatgt atattcaatt ataatgaaaa aacggtcttt ttgaaaggac
     8821 aaatgcaaca taaaaacacg ataaattgca tgtacattgt acacacaact ttaagaccag
     8881 aggaaatcat agatacaaaa acattatgaa ggttatatgt gatacataaa tgcaatataa
     8941 aaacatcgtg gacatcatgg ttcaggttaa gggaaaagga atgaaatggt gaaactgaga
     9001 ggaaaggaaa gcggtgacga atcatgtcac cataaacaga aagatgtcta atggggactc
     9061 tttaggtacg tgaagaatcc ccataacgca tttaaaagac acacatcaga tacccttaag
     9121 gtatccgata gtttattgac ctattgcacc atcacgttca agcttctctc ttatagcctc
     9181 aataatatat gagctaaaat ccagagatgt tttactggtc gctttaagtt ccgcatgagc
     9241 ttcaaaatac gacagcggta tggccttaag attcttagcc ttcgtcctgg cagccacggg
     9301 acgacctgaa acttcctctg caccttcatt tggctgagac ggggctgttg ctgttacggc
     9361 agaagaagaa gtttgtaatc tggcagaatc caccaggttt ttcagttttt cagcctgtgc
     9421 actaaatttc gctttgctca tgagataacc caggatgttt gttagatact taaaagatat
     9481 ccattaaaag cccaaaggat actatatgga tacccaatag acacacctta ctgtttgtta
     9541 ttttcaatca gatgattgat ttcttcgatc agggcaataa cttccttacc tcctgccgaa
     9601 tagcgcccat gaacagactc agtgattccc atcccagtct caatgacatc ctcaaattct
     9661 gcacgagcag aaatgcggct cttcatcagc ttcagatgtt tgaacgatgg caggattgca
     9721 tcaagcttag ggaatttctt cttgttagga ttaactttac agagatacag gtgagcagtt
     9781 atatcggtcc ccatgataga gctgatttcg tccagtactc catcaaaatg catcaaacca
     9841 atacgttcag tcagggagtc ttttgaagga acaataacgc agtctgcaaa ggcaatcgca
     9901 gtacgggtaa gatctgagtc aaatccccca cagtcaataa gtaacgtctt atcgctgttc
     9961 ttgtagggag tcataatttc gattaactca tccactgttt tgggtacttt cacggaaatt
    10021 tcttttcctt caggtctcag ccccagaatg atagaaagcc cggtatgagt atccagatca
    10081 actgtatcag acttcatttc accaacaatg tttacggatg aagttgtctt acccacacca
    10141 cctttgggat gcgtattagc aataacaggc atagtgtatc tccagaatcc acatgtaata
    10201 cccataaaga ccccataaga cacctttcgg atatccaaga ggtactcaat agatgttttt
    10261 ttactctata ccggtcctgc atgtcaacag aatatgaaat accgataata tatccatgag
    10321 atatccgaaa gggatttaaa aaagtcagag cttatggaat tcttttgagc atagacaccg
    10381 catcggactc aggcatcgaa aactgcaccc ggtgccgtgc agccacatcc agtgcaaaca
    10441 ccttcgtgta gacctccgtt gagctgatgg acttatgccc catcagactc tgcaggacct
    10501 tcagcggtat accggcatac agcatatgca tcgcatagga atggcggaac gtatgtggcg
    10561 tgaccgggac agagaacgtc actccatcag cggcagcggc ggcaaccgcc tccccaatcc
    10621 aggtcctgac cgttctgtcc gtcacttccc agatccgcgc tttctctgtc ctgcctgttc
    10681 gtttattacg ccgttccatg gggattttca gtgtggccac catcgtctgc agctggctga
    10741 cgtaccaggc gtcggagagc ggaaccagcc ggtgagtctg ctgaccggcg ggcatccttc
    10801 ctgccgtcct ggcggctttt tcggtccgct gcttcagagt ggccagctgc acaaacggat
    10861 acggaggcgt gagcgaaaaa tccccccgcg tcagcgccag tgcttcatta atgcgtgcac
    10921 cggtgttcca cagtgtggcc agcagcatct tgcggcgcag atccgggacg taatggagca
    10981 gggcgctcac ttcaggggcc agcaggtatt ttggcagttc atcatgaacc atcgacatct
    11041 ggcggagtgc cagcgctgcc ggataatcga tggcaacagg aagctgagcg gcatcccggg
    11101 catggtgtat cggtgccggc aggtgctgca tatcagtgtt cactccacgg ggaaacgtcg
    11161 ggcaggtttt ccacaacacg gtactttccc ggataacgga gctggttacc gccacgcgtc
    11221 cagacggtga caacaaaacc cccgccctgg ttgttttcgg ggagcaggcg atcgcactgc
    11281 tcactcagca tgtggatcac aaacggcgca tcgctgccgt cttcaaatac cagttccagc
    11341 gcatcgcggc catgcagcgt tcccttactg atgatgacgt actcagcccc ccgcatctcc
    11401 ctgagtaaat gttttgccgc atccgggaca agcagcctgg ctgcgccggc attccagctg
    11461 aggtagacat atccggcctg cgcctgtaca gactgccagt aatccgttcc ggcgacagcc
    11521 tgaccttcat tctcaataaa aaacatgggt gactcctttt ttcttttccg ggcagggacg
    11581 gtcatgcgat aacgtcatcc atcatcactg cccttaccgt cacgccgtct gataacatca
    11641 tgccgttatc atcatcacca tgataacaag catccatata accacgccgt taaattaccg
    11701 gagagccgtt tccgtcagat ccgactttcc ggaatatcac aactttccgg aagtgtacca
    11761 cgctgaggaa ataatcatat cgctgttttc cgcccctgcg cggcgcatca caggtcaggg
    11821 agatgacaca acatatagtg tcaagagcag ctgaattcag cactacatgt tgtgcttaca
    11881 gggtgtggcg ctgtgcggag ggggaggaag atccgtaacc tggggttttc ggatctcaga
    11941 aaaggaagtg aacagaaaac agacagcgaa gcatacgaat tataaaaacg tatgcttcgc
    12001 agtatttaaa gacactcaaa accaggatag agtataagta ttgcagcaat gccccctcac
    12061 atgaaaaggt acgcgggatg agtgaaaaag aacatgacat gacaaacgac ggtggagaga
    12121 gcgtaacgta taccctgcgg aacatccccg ctgacaccga ccgggtgatc accgacctgg
    12181 caagccacgc ccgtaagcca aaggcgacgt ttctgcgtga gttcctggaa gacagcttcc
    12241 gcgacgtcat cgacagcttt gccctgaaga acccgctcat cgcctccctg gatgaggagc
    12301 tggccagtta tctggacgct aaggtcatgg agcagcgcta ccagagccat tttatcaccc
    12361 gctggaatca ggaataccag aagctgctgg ggatctcgac ggaagaggag ctccgccggt
    12421 tggtactgaa caacaccccc ttcctgcagg tccgtgccga ccaggtgctt aaggggtgga
    12481 aaaatatccc gcgcgggatc tccctgacct tctccctgtt tgccgagatc gccggccggg
    12541 acagggaaac catcgaccag gcctggaaaa atatcttcta ctcgcaactg agggaaaaaa
    12601 aacaccgctt ttaccaagat atcgaggcca tccgcgccct gaaaaaactg cctgccctca
    12661 ccggcgacag ctggacgcgg gatggcatca cggtgcgtat ctaccgcccg gaaaattacg
    12721 cccgcggcgc atggcgcgtt acactgagcc tcccggaaaa ttacgccacc cagatgtgga
    12781 acatcccgtt cccggagctt gaataccgcc tctttaccgc cgatccgggc tacagcgccc
    12841 tgatcagcgc cgaacccgac aggtgggaca aggccttccg ttttgtggac ggggtgtgtg
    12901 agcttcacct ttacaccaac ggtgtggaag aagatcacaa tcccaccccg ctcggggatg
    12961 tcgctcaggc gctgatcaac gtggtggaag aaaacctgct gtaacggacc cggatctgcg
    13021 ggcacaactg catcatcagg aggatgcaat gaaaggacga cagagccgct atgttaccgg
    13081 cggagagagt ttcgcggaga ttgcccgtct cccttcaggg gcggtggtga ggctctgtct
    13141 gaacaccggt cttgaggatg cgctgcggga ggcctccaaa tcgctcaagt cagccttcac
    13201 ccgttccggg cgaaaatgcc ggctgtcagc gggtacggcg caggggccgt ttaccggacg
    13261 ccggcagggc gtggccacac atctcttcgt ctcggtactc tgagggggca gggggcaaaa
    13321 aaagtaaaaa tgtattcgcc aggttgcccg gaggtgaagg aaaatagaca tacagcagaa
    13381 cgacggatag cactttttgc taaatggaca tcagtattac tatgctatag ttgctttaat
    13441 ggataagtgc gccttgacaa aggcggtgat ttctgttaac attactctca tagtattgtt
    13501 ccgtcccgct ccaccccaac aagatccgtt tatttcccgc cagactggtt atcaccattc
    13561 aggcccggat ttttttggat ttttttccgg ggagcccccg gaacgagctt aaaatcggta
    13621 tgacaaacag gaggatgcga atgaacacat cataacagag ctgaaagata aaacattctg
    13681 tacggcatta acagcgttca cgtgtgtgag gcgccgggtg ccttttgact taaaaacgag
    13741 gttattgaga tgaccaaaat caagacagtt acttttgtaa atacttaccc gggagggttc
    13801 tatgaaaaac ttgttagaca ccgagggaac ggttctattc ccattccaga ctgaaatcca
    13861 ttttatttgg acgattttct ccaccgttaa acgcctggtt atcggaacca gggaccatat
    13921 ttgccagaag caatactgga gcgcctgtct ctgtattttg cttcttatgg cctatgtggg
    13981 tctctgtgct gcggtggtct ggtttgtagt gccctggctg aaggccttta tagtgtcgaa
    14041 atttgcggtt tcggcactat gggtcacgcc agtaaagcgc ggactactct ggggtatcgg
    14101 taaagtggtt accgccactt gccgaagatt tactctgcta aagtaagtag ccgcaacgct
    14161 acacgaactg atggtgaatg tcaacagata ctcaccatct ccttacggcg gtggtccctg
    14221 tgaccactgc cgctttcgcg tgggtgcaac acggcaaaac tcctctgtac aacaggggct
    14281 cccgccgtca ttttccggca caggtgaggc cggaattcgg actaaaacgt aaaccgcggg
    14341 ccagtccggt agcgttcact atcggccagc attctctcaa ccagagagaa atccttttca
    14401 ccgcagaaca cgtactgctc cgcgaactcc acctgcaggg aattgtaatg cgtgacatgc
    14461 tccggggtaa gcggcataag aagatagtcc gtatacctcc ggatatgttc cagtctttca
    14521 aaaggccgaa tgtgacgggg gataagatgc ggcgccctgg cgagcagatg ttgaagatgc
    14581 tgcttttgac gaaccatctg ttcacggatg gaggggcaat acatggccag catcagcgtc
    14641 gatgacaggg ggaggtaaat ctgtatcccg cgaacggcca gaccaagatt accgtacgga
    14701 ccgaagtcat tgctgttctt aagcactacg ggattgtctg atacgtaaaa cggacgttct
    14761 ggcctggttt ccagaaggta ccagtccttt ttcagcagga gatcaatatg tgacacctgc
    14821 tggacgagct gccgcagaaa aatatccttg gtatcgcccc cggacgacag gcccagggtt
    14881 tccatggcct gctctttagt cgagccgatg gcctccatct tatcggtaag caccgaaatc
    14941 atgcctgaga tacgctccag ttcaccgaag gttcgtgcgc gctgcacggc aacaaacacg
    15001 gccagcctgt agcgttcatc atctgtcagc ctgcgaatat ccctgtgcgc cagcagggct
    15061 gcaattaccg gtgccgcgtg gtgctcgtaa atccccagca gtggttcaag actcagacgg
    15121 tccgggtgat tatcgaggtt gtagaacgtg ttgcggacgg tcgcgtcatc cggcgtggtg
    15181 gcataggcgc gcccggcagc cttgtcaaag gcgtataagc gctgccgctt accctttccc
    15241 ggcgtactga aatgcttcag taaaaaacgg ggaaccgtat gctgcctgac cacatcggtc
    15301 tgcagattga atggatcggc cataacttta tctccctgaa aagccacgca agcgcatcgg
    15361 tgcatctgta ttgacggcat gggcagctgg tttgcagctt agcattagaa aacagaaccg
    15421 ttcgataata cctgtgttta acgccctgtc agtaccgatg gaatttaaat ctccggaacc
    15481 gccggaaacc ggatctgcac gacgggaaac gggcaactga tatgataatt tagcaataca
    15541 cataaaatca tatcagatat gattttcatc attataaatg aaacaatatc tgatattatc
    15601 agaaactaag aatacataaa tcatacatga catgattttt acctgtcaga acattatcag
    15661 atggagcgtg caggatggat gaagaaaggg tgttttcact cagctatgag caactaaccc
    15721 gaatagcgga aaaaaatatc cgtgaatgtg ggctggacaa ccagagcgcc aggtgtatta
    15781 gtgaattgaa ggcctcagct ctgctgtggt tgtggtatga actggccatt catggtgcgc
    15841 cacagaacaa tcacgcacag gcccgggaac gcatcgatac cgattatcag cgcctcaaaa
    15901 aactcatctg gtcggagggt gacagttaat gactcagagc cgccgaccgt cgccactgca
    15961 gcggagagtg ctgattgtgc tggctgccct tgatgaaaaa cggccgggac ccgtgctgac
    16021 gcgggacata gaacgggtgc tggagcgaag cggggaagcc ccggtctacg ggccaaacct
    16081 gcgcgcctcc tgccgtcgtc tggaggatgc cggctggctt cgtaccctgc gcgctccgaa
    16141 cctgcagctg gcagtggagc tgacggacgc cggccgggcc gtggcacagc ctctgctgct
    16201 ggcagaacag gaccgcctcc gggcagaaca gcgtgcggca gaagtcgtcg tactgccgct
    16261 ggtcccggca gcaggtctgc ccgcagacgg cacttctgcc actgatctgg cggtagagct
    16321 gaacggcata acgtatcagg cctgccgtgg ggattttgtg gtgcgtctcg acggcagcac
    16381 ctgtctgcag ttatggaata aagaaggcag ggtggtatgc ctggagggcg atccgctgga
    16441 agtggcgcag tggctgcagg cctgtcatga cgcaggaata gaggttcgcg tacagataaa
    16501 tgaaagtagc gtcccgtaaa aacatgcctg catggtttac acgtcatccc atggtggcag
    16561 ccggttaact ttcctgcgcc accagtggaa agaggccgtg agcagtccga acaaaaaacc
    16621 acccgtgata cttataataa tggcctcacc tgccaccatt cctgacggcc cccagtagat
    16681 aaaccacatt gcacatcccc aggaagtgcc ccataagccc cccgtcagca ccgcgacctg
    16741 ccagaacggc ataaagggta atggcggaag ccggataccc agccgccaga gaatacgcag
    16801 aagaggaggg gcataattgc tccgccacat ctttttgctg tccatcaggg caatggcccg
    16861 ggcttttttc tgctcaaagg tcacaggagc acctccgttc catgatgtaa acgtgacaca
    16921 gcttattgtt attgattatt attatcatgc tgtgattatg ttatcacgca ccggtacacg
    16981 ataacaataa aggagaaacc agatgaaact ggatatatct gtaaagtatt tactgaaaag
    17041 cctgatacca atcctcatta ttcttacagt tttttatctg ggatggaaag ataaccagga
    17101 aaatgcaaga atgttttatg cgttcatcgg atgcattatc agtgccatta cttttccttt
    17161 ttcaatgagg ataatacaga aaatggtaat aaggtttaca gggaaagaat tctggcaaaa
    17221 agacttcttt acaaatccag ttggcggaag cttaactgca atatttgaat tattctgttt
    17281 cgttatatca gttcctgtgg ttgccattta cttaattttt atactctgca aagccctttc
    17341 aggaaaatga aagggcatca ttatgtgtca gataccaata agtttattga cctgctcaat
    17401 aaacttatca ttaacaagcg cactcacagc cgccataata attgcaaaac ccagaatacc
    17461 tacaggggtt cccagcatga cggaaaacgc ccatgctgta actgctgaag cagctcttcc
    17521 tgctgccagt gattcaagtt ttacaaaaaa tggacgccag ttgtccgttt ttacagcctt
    17581 ttgtaactcc acagcaacat cataacgatc aataactttt ccggtaaaac caaatgcctt
    17641 actgaacagc ttaaaattct ccgccatatg gacctgatta atagcttcca gggctttaga
    17701 aatggccatg cgatcttgta tgttatattt cttgttcaga ttattacgga atttatcaaa
    17761 agcgttcagt gcatcatcca cactcttaat ctgtttccct tgagatacag aagccagctc
    17821 cttagcaagt tcggatgctt gtttaccgaa ctcatcatat atcgttctgt agaaatcaga
    17881 ggtgagtttt atggcatcct ttaccatatt tatttcatcc ctttttcttt tctcttcagc
    17941 tattttctga ttgatgtcag caagctggct acggatattc tctttttcct tcaacagggc
    18001 attaagagcg tcatttgcat gcttttgttc atttgttctg gcactgacgt tatttctcgc
    18061 cgaatttatc gcagattcag cagaggtgat tttatttctg gcatcaagca atcgttgccg
    18121 taacttatcc cattcagcaa cttcagcatt ataaattttc ttgtcacctt ccacatcctc
    18181 tgtcaccacc ggattccggt cattcagaat attgcgttta tagtccagac cggaatgggt
    18241 cagaagataa ttcaggtttg cacggttatc gatgaccgcg ctggttgtga tttttatcgt
    18301 accggcaaac ctcggatcac cactcacaac aatagaatga tttgttgatg aacgccccgg
    18361 gaatttttca ggggaaacta tcgttttgcc gtcaggcgtg tttttctgtt gcgtcagctg
    18421 ttgttcagct ctggttaatg cattacgggc cgtcttaagc gattccgtca ccgaactgag
    18481 tttattccgg gcctctgaaa tggccctgtc attccgtgtc tgctgcccga gcagacgggc
    18541 ctgttgtgca tcaagaacgg caagggctgc cccgccattt ttttccagct ctgacaaccg
    18601 cgtgtccaga ataccttttc tgcgctcgta ttcagcaagc atatcagcgg ccttcttgtc
    18661 tgcgttctgc gcatccttct gccgtttttc agcctgttcg attaacgacc tggcctcgcg
    18721 gggagatata cgaagagaat cagcatcagc aaacagcatc cggctctctg tatgcagccg
    18781 gagggcatca tatccggcga tttcagtacg gaatccgtat tctctcagct ctgcctgcag
    18841 gagtctgaac tcctgtcgtg taatatcagc ggcatctgcg tttccggcac ggagtgtttt
    18901 cagtgtgttt tcatcttttt cccttgcagc aattgccgca gaaaggcgtt ttccagcttc
    18961 agtacgctta ttctcattct ctttctgctg cgcgctgagc gtgtttttgt acgctgtgat
    19021 ttccttgtcc cttttttcga tatcacttcg ggttggggaa tcatcgaccc actcgtttcc
    19081 accccagatg gttttgttcc cgaaggaact ccatgcaggc ggggtaccat gaaagacatc
    19141 gacacgtgga gggtttacat aaatatcaac ggccataatt tcatggccat ctgaatcata
    19201 tcccagcgat tctgcaccgg gatttgtaat acgtacaggg tcagacattc ggtatctcct
    19261 tcatcctctg aggaaaacaa atgaattaac tgcttacgta tatggataca tatacagtac
    19321 taatttatta aggagaaaat ggcatgtcaa gatttaatga tagtaattat cattaacatg
    19381 aagtggtgtt ataccgttca gaaagcctgc tgacactgtg gcagcatcac agtctgggta
    19441 ttctctggtg caatacaaaa accggacttt cccgtaacaa cagacaatgt tacgggaaaa
    19501 gttacagaac tgagccacaa aatatctcag tacctgagat gcataaaaac accgtacata
    19561 tgtttcttaa aagggggatg atcgttatcc agccatttcg gtggatcggt atgcagaaca
    19621 ccaatctgct tcatgccggt atgctgcctg gattttctcg cgcagtaatc actgtcttca
    19681 tatcccatcg tttcgatgac cactctggcc gtctttccgt caggagccct tgctgtgaca
    19741 atgaaatccg gcagtacata tttcttttca ccattaacat aaacttctgt atcaaacagg
    19801 ggcctttcca gacttaattc cggcgtacct ggctttcttt taagccagct ggccacattc
    19861 agaagagcag tgagcgtgtt tcgttccagt ttgctgtcca caggcaccgg acatgtcagt
    19921 tgataaagag catgagcata cccttcactg cagatgattc tgccatcggc actccggcaa
    19981 aaagcaagaa taacccagta agggggacgt attccgccct gcgcttcacc attaatactg
    20041 atcccccgca caggtctgat gacctgaata ttaccgttcg cccagtgaat ttccacagca
    20101 tcacgacaga tgtgctgcgc cataaaaatc tgaaagaaca tatgctttct tctggcaggc
    20161 cagtgtcgtt ctgaattctc cagcgtttcc attaaccgct cctgagacct ttcagacatt
    20221 cctggccgga aatggataat ttcagataag gctcttcccc gaatatactc ctgctgtagt
    20281 gtaacctgcc gtatggcatc aagacactca cgataattag tccggatacg gggattagcc
    20341 agggaatcca gtttattcag ccctgcatct tcaataagcg acaggagtaa acggccaagg
    20401 cgcggacgtt tcttgcgaac ggaggagcgc tcaccattat ggtaagcaga gcgggcctgc
    20461 agtctgataa cagaattact gtcatcaggt ggtaaaaagt ttctgtaatt tatacgggtg
    20521 ctaacgggat gccggcttgc tccgaaggat gtttcctctt catttccgga taactgacga
    20581 tacatggggc attcaggtgc atgctcatga ttaaaactgg acagataaag agtacccgta
    20641 ttgtttttaa gagcagcaaa attcatggcc ggcgaatctc ccggaataca gtcacactgt
    20701 aaccatgcat catactcacg catttcccgg agaagatcat tggccaggtt atgttgtgcc
    20761 atggttgctg gccttttcag tccacagacc gtctttactg cagtctcttc ctcactgcta
    20821 agctcccgaa tcactttgcc ccggggattc ttctttacaa gtatcattgg cataatgaaa
    20881 gcactcctta tgtacatgct taaacagtaa gtaaaatata caaagaaccc cccatgaaac
    20941 aaaggcagag atgaactgtg cgccccgata tcttcttctg atatcctttc aaatgaattg
    21001 tttttattta tatcatgaca acattaaagt gagagaatat atcaaccatg tttctgtcag
    21061 ggttcagtag aatatcaatt cgactgtcac gccataactg taactacctc gagtataaat
    21121 attcagagaa tggacttatt agccatgaaa aatcacctga tgggaaattt actaatttat
    21181 ggattgtgat attattaaag agtaaaacca gccagccgag gtatttatgg gaaaaaatca
    21241 ggaagaaagg aaaactcccg tcattgtggt gaaaaaacgt cgtacttttt cgccaccgtc
    21301 cttatccgaa aaaacagata ttatcgcacc tgtatttact gaacagacgg cagaatcagc
    21361 ccccgccgga ataaattcat ctgccgtcga aacacatatc cccgaagcgc cagccaggaa
    21421 gaagaaaaag aaaagacacc gttttccacg tccctcccac tggacccgtg agtatactca
    21481 cgagtgcgtg gaaaaaataa aggccctgtt tcctcatctt cgtgctgaag gtggtggatt
    21541 tattcccctg aaaattggca taagcaatga tatttcggca tttctggcag aacacccgga
    21601 gacagaactg accatggacg aatggctctg tgcagtgtcc tgcatcacat cccgccgcgt
    21661 atatctgcag cggacagctg tcgccggcgt cccacgttat ggcctggatg gtcaccccaa
    21721 agggcaggtc tcagactctg aagcacagag tgccggtcgc agactggcga cattagaaca
    21781 aaagtggctc aggacgcagg cacagcagga gaatatttct gggcaataaa cactcctgat
    21841 aacaggtgga cattacgtat cagaaaaaat gggaagagaa tgttatgtgt gattcagaag
    21901 cggagaagat tttattcatc ctgaataaac atgaacgggc atacagacgc ctgatccccg
    21961 tatatatttt tttagctata gtattatgga ttttacctat tctcctgatt gtatacctgt
    22021 atttctgtat agttgacaat gagtttctca gaatcggttt ctgtattgca tggacagcat
    22081 actactttct ttcgcttttc aagtattttg taaaatactt ctttcctctc gatcgtgaag
    22141 atttgcttaa cctgatggat ctcgccaaag attctcctga tgcgaaacag gagcttcttg
    22201 agcgtctttt gtccggaaaa agaatgaccg ggcgggatga aatggatatc aagcgcattc
    22261 taaaaacaaa ggaagcactt gataacatca ggaattttat tgataaacag cagttaacga
    22321 atagtgatac tccgaagaaa agcacggtcc caaatgaaga taaaaaaggc ataagatgat
    22381 gctcacagga aatggaataa acactgtaac tgtcaatgga aaagtgaaac acatcacaga
    22441 actggatgat ataacacttt gtttagagtg gacaaaacta cgagaggaaa acaatcgtct
    22501 ttatgaaatt aataaccagg caaacagagg atggcgtggt ttcatcctaa gattgatagg
    22561 ggttaacctg ccagataaaa ggactgagtt tacacaacgt ctcttattga cgcgcaaaat
    22621 ttcgggttcg gtcatgaaaa agtaacaaaa ccgcgcaaca gacataaata tgacagtagc
    22681 catgaaaaag cagagagaga cagggtgata cagaaaagta actatttttt agctatagta
    22741 ttattggttt tacctatttt tgtgatttta tttctgtata tttgacaatg agtctctcag
    22801 aatcggtttc tcgaagtgac gagcgatcag aatggagaca aataaattcg ggaaacgaac
    22861 cttcacggta agttttgtca catttatcat ccctaaacat gagaattatc accaaaacta
    22921 accacccaac caacagcagc gccgggagca gcattataaa atcatgtaca gagttgcgtg
    22981 acgacatgat aagttctcat tctcaatatg ttctttacca gattatacca gtgatacttt
    23041 aacgcaccat cattaatgct aatgtatgca tacattaaca ttaatgatga aggagaagaa
    23101 caacaatggc agtcaaccca cttatttttt ggatttctta attttcgtct gataaagccg
    23161 ctttacccgt tcaatttctg ccagcgttgc ggaccagtgt agttcggcgg catgacgata
    23221 tctggcagcc attttttcca ccatctccgg attgctgtcc gcgctgcggt gctgacgccc
    23281 cttctcccca aaaacttcaa caaccagcca ctggcttccg gttctgccga acaatgcctt
    23341 gcacaaacgt ctcacagtta gcggggttaa atctggatcg ggatcgtagg caatggtagc
    23401 aaaaataaag cgcagcattt ctgatgtctt gtaacgcttt acgctggcag ccaggcgtga
    23461 ggcacgttgc ggtgcttccc agcggtcgtt actgtaatta gccacttcag ccagataatc
    23521 cttttcaagt ttttcagccg tgtagcacct gccgcctaaa aaaagccgca tggcctccct
    23581 ggttttgtct ctcacatatc atccctcatt aatgctaatg tacacataca ttagcattaa
    23641 catgctgaca gcaacaagcg gcggatttac gtctggataa cgtaaatgat tgcggtatac
    23701 aggccaggcg gtcagatttt cagccgtgcc tgttcctgca gcagttccag atgatatgca
    23761 gcaatagcgt ttaccacccc tgccgtcatc cgtgcgtcgg cgatcgctga gtgtgcctgc
    23821 ccttcccagg tcagtccggc ctgactggca gcacaggcca gtgaaatcgt gccatagcgg
    23881 ttggtggccc cataatatcc tgcagccagc tccatcgcac aatataccgt cagttcttcc
    23941 agccagtcag ccggatcgct atgtgcggca gcagtctgtt tcagaatgcg gatgtcgaac
    24001 cgggcattaa agataattac tggtcggtcc ccgattgcat gacgcagttg ccgcgccaca
    24061 tctgtccatg atggtgcgcc gcagagagcc tgctcgctga taccatgcac agcggcggcc
    24121 tgcgccccga tggcgaccgt gggcttcagc ctggtttcaa ataccacctg accggccgca
    24181 tcggtcaggc cgatttccag ggcttccgcg gtattatcca gtcctgtcgt ttcagtatcc
    24241 agaaagagcg gtgccagact cagccagtca tatgcctgcc gtgccatccg gccactggtg
    24301 ctgttaagtc tggataaaac ggaaagtcgc tggccagcca gctgttgttt actcgtcaga
    24361 gggcgtttct cacgcatcgg aacacagtct gcaattcggt atacagcgaa cctgccacca
    24421 taagtgtttt tataccactt aaccggctca gcaccggggg ctgtcacgaa cggtgcaata
    24481 gtgatccaca cccaacgcct gaaatcagat ccagggggta atctgctctc ctgattcagg
    24541 agagcttatg gtcacttttg agacagttat ggaaattaaa atcctgcaca agcagggaat
    24601 gagtagccgg gcgattgcc
//
LOCUS       DAJTVJ010000060        24279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000060 DAJTVJ010000000
VERSION     DAJTVJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.060"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..201)
                     /locus_tag="OO448_002316"
     CDS             complement(<1..201)
                     /locus_tag="OO448_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6337509.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAV"
     gene            complement(204..1871)
                     /locus_tag="OO448_002317"
     CDS             complement(204..1871)
                     /locus_tag="OO448_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6337510.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(1868..3976)
                     /locus_tag="OO448_002318"
     CDS             complement(1868..3976)
                     /locus_tag="OO448_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6337511.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(3963..4784)
                     /locus_tag="OO448_002319"
     CDS             complement(3963..4784)
                     /locus_tag="OO448_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6337512.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(4943..6772)
                     /gene="glmS"
                     /locus_tag="OO448_002320"
     CDS             complement(4943..6772)
                     /gene="glmS"
                     /locus_tag="OO448_002320"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6337513.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6934..8304)
                     /gene="glmU"
                     /locus_tag="OO448_002321"
     CDS             complement(6934..8304)
                     /gene="glmU"
                     /locus_tag="OO448_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6337514.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8655..9074)
                     /gene="atpC"
                     /locus_tag="OO448_002322"
     CDS             complement(8655..9074)
                     /gene="atpC"
                     /locus_tag="OO448_002322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6337515.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9095..10477)
                     /gene="atpD"
                     /locus_tag="OO448_002323"
     CDS             complement(9095..10477)
                     /gene="atpD"
                     /locus_tag="OO448_002323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6337516.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10504..11367)
                     /gene="atpG"
                     /locus_tag="OO448_002324"
     CDS             complement(10504..11367)
                     /gene="atpG"
                     /locus_tag="OO448_002324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6337517.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11418..12959)
                     /gene="atpA"
                     /locus_tag="OO448_002325"
     CDS             complement(11418..12959)
                     /gene="atpA"
                     /locus_tag="OO448_002325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6337518.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12972..13505)
                     /gene="atpH"
                     /locus_tag="OO448_002326"
     CDS             complement(12972..13505)
                     /gene="atpH"
                     /locus_tag="OO448_002326"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6337519.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13520..13990)
                     /gene="atpF"
                     /locus_tag="OO448_002327"
     CDS             complement(13520..13990)
                     /gene="atpF"
                     /locus_tag="OO448_002327"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6337520.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14052..14291)
                     /gene="atpE"
                     /locus_tag="OO448_002328"
     CDS             complement(14052..14291)
                     /gene="atpE"
                     /locus_tag="OO448_002328"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6337521.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14338..15153)
                     /gene="atpB"
                     /locus_tag="OO448_002329"
     CDS             complement(14338..15153)
                     /gene="atpB"
                     /locus_tag="OO448_002329"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6337522.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15162..15542)
                     /gene="atpI"
                     /locus_tag="OO448_002330"
     CDS             complement(15162..15542)
                     /gene="atpI"
                     /locus_tag="OO448_002330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6337523.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16159..16782)
                     /gene="rsmG"
                     /locus_tag="OO448_002331"
     CDS             complement(16159..16782)
                     /gene="rsmG"
                     /locus_tag="OO448_002331"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6337524.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16846..18735)
                     /gene="mnmG"
                     /locus_tag="OO448_002332"
     CDS             complement(16846..18735)
                     /gene="mnmG"
                     /locus_tag="OO448_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6337525.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19114..19557)
                     /gene="mioC"
                     /locus_tag="OO448_002333"
     CDS             complement(19114..19557)
                     /gene="mioC"
                     /locus_tag="OO448_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6337526.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(19647..20105)
                     /gene="asnC"
                     /locus_tag="OO448_002334"
     CDS             complement(19647..20105)
                     /gene="asnC"
                     /locus_tag="OO448_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6337527.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20257..21249
                     /gene="asnA"
                     /locus_tag="OO448_002335"
     CDS             20257..21249
                     /gene="asnA"
                     /locus_tag="OO448_002335"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6337528.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(21254..22705)
                     /gene="viaA"
                     /locus_tag="OO448_002336"
     CDS             complement(21254..22705)
                     /gene="viaA"
                     /locus_tag="OO448_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6337529.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22699..24195)
                     /gene="ravA"
                     /locus_tag="OO448_002337"
     CDS             complement(22699..24195)
                     /gene="ravA"
                     /locus_tag="OO448_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6337530.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         6004 a         6312 c         5775 g         6188 t
ORIGIN      
        1 aacagcgtaa cgtcctgttt gatgtagatg tcttgctatc acacctaaat gcgagggcag
       61 acctaaggta gcgaccacct tgcggttgcc atacacctca tccaacagct gcttaggact
      121 aacaatccct tgataaacgc ctgcccgtgc aatagtgcta taaatcagct cattcgagta
      181 cggaacagga aagtttctca tggctaacct gcttgtttaa ataagctcgc catatccact
      241 attacccctt tcccttttaa ccgttcatgc atggtttttt caggttggcg ttgtgaatag
      301 atataacgta aatccgtatc aggcaagcta tcccagtcgc tgggcttgac tacctttata
      361 gccgaaacct ttttactctt ggagggcttt tctagttctg acactaccgt ttcgccttcc
      421 atcaaccact gcaaaacaag aggcagtaac ttttgtcttg tcatcgttgg attctggcta
      481 aacgcttttt taatagtggg aattaacagg cttgaatcgt aatcctcttt cagcatcaga
      541 tataaatgac gctgatcttc ggtatctaac tcttgaaggg ctttttcttc tggtgtttgt
      601 tcttgtatcg ctgcgatatc tagctgaagt tggattaacc gtttatcaat ctcgggaacg
      661 actagatcag aataacgagc aatgcgttct gggatacccg agcgtaatgc ctctagcatg
      721 gggtgcacag gctttaactc atcttgatac acttgccgca ataaaccagc ggtaatacgc
      781 tcattgccta aagctagcgc acggagctga gcgagtacaa aaagttttac tacaatgtcc
      841 atcactcctt ggcttagctc ataccacaca tcacggacct catccgataa cagcgcatct
      901 ttgcgttgta aaagctgtaa ttgccaaaga ttatccgtaa aagcgatcca ctcttgattg
      961 ggctttccac gttgcgtttg ttgtatagga tcccagaata tagctccaaa ccctgcccct
     1021 ctacgtgcag accgcaaatc agcctcaaaa atctctcgtg ctttaggggt accaatcaac
     1081 atcactggta cgccaataat attcaccatc gtcacaaaaa agttcagcat ctcttgagat
     1141 ccacccgaac gagagcggct taaatgctga atttcatcaa taaccaacaa ccctaaagca
     1201 tgtgcattgg ctatttgcga catcaaagcc aacatggttt ctataccatg acgttttaag
     1261 ccataacgac gctcatagtt cgagcccaag gctcgatcca acgctctgaa aaaattcaag
     1321 cagatttctt ttagcgaacc attatgcgag cagtctattt tcaaatacac cacctgctct
     1381 acattgagtt cacgatggta aatcacctga ggatacgtgg ctagaatacg atgaagagag
     1441 gtcgtcttcc cactaccaga acaaccaatt aacaataagc tttgtgccgt agatcgtgcc
     1501 tcctcaaagc gaaatgtctc caactctccc gtttgaacac gctcataacc attttgtaaa
     1561 tgcttttgta aatctcctgt tttaggattt ctgcctacgt agccacctcg aatcatgacc
     1621 gaaatacgct cacttagtag caaatgcgta cctaatggct gaaaatagtc atctggaata
     1681 cgacaaatgg tatgagctct gataacacgg gacttttgca agtcagagga agtaagctgt
     1741 aaagaggatt tcagtgatgc agcactattc actgactctt gtaatggtgg taaggcctcg
     1801 ataaaaggat tatcacgata agcctctacc cccgtatcac gataaactgc ttgaatccgg
     1861 gtagcactca tgactcatcc ttttctggtg gatcctgaaa taattcaggc acgtatgtgg
     1921 gtaggctgta atcttcttga tcatccgctt ccactgcgtt gaaaggaata actttagcct
     1981 catcacctga agagcttggc ttcaaatgct ccgcacgttt tttacgctcc gaggtcacgg
     2041 cttctttttt attagtttta atctgcttaa tgcgtgttga tttgggctca gtagtactgg
     2101 gcgttaactt attcgctttc tgaatggttt gctgaataaa cgcctcaagc tccctgcgtt
     2161 tagttaactc atcctgcttc gcattggctt tattgtgttt ttcttgtgct tgtatatccc
     2221 aaacctccca aaatgagaga cctttaaact gccgactacg ttccgtcaga ttacagcgcc
     2281 aaaatacacg gctgccaact tgcggaaaca aataaatcgt atcaaccagc actgggtcat
     2341 aagccgcttc taaatgttga ggtctagcta tatcagtgct ccgctgcaac caaccctcac
     2401 gcagaatctc tgaccccgag taatacaaac cccacaaatt aacgccaaat gaagaaatag
     2461 agacctttcg gcgaggcagt aacgctactc gcaactgctc ttgctccaca gcccttaaac
     2521 tacctgtacg atgctgcata ccccattgcc atagctggac aggaatagac ggtaaatctg
     2581 taggaaaatc agcatctcga tcgtatttat ccatcaccag atggttattt ctgaataaga
     2641 tcgtacgcaa aataatttgt gtgaactcaa ataccgacag agatgcatct aacctatagt
     2701 ctgtttcacc atggcttttg atccgactgc cctctacaat gccaggtgca aaggacttaa
     2761 actcggcttg tagtgttcta aaagtgcttt ccactatgcc tttagcatcg ccacgtctag
     2821 gtggagcact ttccactcgc acattaaaac tagaaactaa ggcttcgacc tgatgactca
     2881 ttaattcgcc acggtccgct agcaacacat ctggcaaacc tacacacggc cagtctgagc
     2941 tactaatctc aatatcatgc tgggcacaaa tggccgtttt gtcagagcaa gcatttacaa
     3001 aagcctgcat cgccaccaca taagacggat tttcaaagcc gatataaaag cccgtgatca
     3061 tccgactaaa cacatcaatc acaatgtaaa gcgttggtct tcctatgatt ttttggcgat
     3121 catgatgatc cactaaataa atatcagcaa tcgtggcatc aatctcataa cgactcccag
     3181 ggcctaacgc ctgagaagtg gctgtactac ttaagggtcg tacgtctttt ttatataccc
     3241 ctgctttaac tctagacttt aagcgctgag ctttagggta ttctcgatca taaaaataac
     3301 gaaactgacg tagtgttggg taatcctctt ggggaatgcg aggaaaatac tgagcaaaca
     3361 agtccacaaa tcgtctatag gcaacggtgg tctttgtacc tttttgattt aacaggtgct
     3421 tttctatggt caacctaaaa aggcgttcaa tctcgggcgt taccttggtt ccttcaccct
     3481 taccatattc tctggctcgg ccaatctttg ctgttcctgt cgctgaacgt ctttcccctg
     3541 gtgcaccgct gtttttgtag tcaggaatta atgcattagg cgtttgacca cgctgccagt
     3601 aacggcgtaa caacttataa actgtagcct tagtaacctt atgttcttgg accacatgct
     3661 caacgagttc gcttctgact ttagggtcga aacgatcctt actattaata ataggaagaa
     3721 tttttcgata atcctcctcg cgcttctgga agctgacaga atctacagac ggctcttcta
     3781 aatcaagatg tacataaggg tcatcagttc taactaagcg gccttcatct aaatactgca
     3841 ttagcaacaa ctcagcccta gcttgtggaa ctcctttatc agcgcttatt tgcatccaga
     3901 caacttggcc atcctctata gccaaaatgc gatacggatc attatcaaat agcacaacct
     3961 cattaatttg ccacatagcg caactcctcc atattcacta cttggctaat acagagatct
     4021 gcacacttat ttgccctgaa agacttataa atattgaact taataaaacc atttgcggtt
     4081 aaggctcgta tctcactcaa tgttttgcct aactccaaat cataagcaat atcaacctgc
     4141 ttacagacat tgataatgtt ttcatctcct ttttcttgca ggatatgggc caatggggat
     4201 agttgtgcta aaagctccgc agaaacttct tctgttttca ctgaataaag ccattcaata
     4261 ttttctttta ctacgggatt tatttcttta tcagtaaaaa tgaaccaagg aatttgcttt
     4321 tgctgccaat agcgacgctc tagttctagt ttttctaagg tacgctcgtc ttgtaaggct
     4381 gctgcaggtt tgacttgaat agcaaactgc tcaaaaggac catctttgca gtccactaaa
     4441 aaatcagtag acataacctg atctacacca cgaataacag gatgcttaat accactatct
     4501 attgcaatct gcctggtatc actaggtaat aaggggaact gctcgcgtat atctagcacg
     4561 ctgctctccc actcaagact gagaaaaaca gcaagctcta agtcagatag caaatgatgg
     4621 actcgtcccg tcttatgaga ataaatacgg tgggaacgac ctgaagaagg aacttcttgt
     4681 actgttagcc atggaatata gtctttacca tgcccttggc cacgcccctc tttaatacga
     4741 cgggcaattt gcacttcaga aaatgaagag tttgctttag ccataacaaa agtccagtat
     4801 gctttttcac agcataactg gactgatttc agtttacaac tattctgtct agtttaagac
     4861 tttattgtca tagtttagat ctattttgtt cagtttaaga ctttattgtc cgcccacagc
     4921 ccttttttat ctatgatagt gattactcaa ccgtaaccga ttttgccagg ttacgcggct
     4981 ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc cagcagctgc agcggaacgg
     5041 tgtagaagat cggtgcaatc acctcttcca catgcggcat ctcgatgatg tgcatgttat
     5101 cgctacttac aaaacccgca tcctgatcgg cgaagacata caattggccg ccacgcgcgc
     5161 gaacttcttc aatgttggat ttcagttttt ccagcaattc gttgttcggt gcaacgacga
     5221 taaccggcat atcggcatca ataagcgcca gcggaccgtg tttcagttca ccagcagcgt
     5281 aggcttcagc gtgaatgtag gagatctctt tcagcttcaa tgcgccttcc agtgcgattg
     5341 ggtactgatc gccacggccc aggaacagtg cgtgatgttt gtcagagaaa tcttctgcca
     5401 gagcttcaat gcgtttgtcc tgagatagca tctgctcaat acggctcggc agcgcctgca
     5461 gaccatgcac aatgtcatgt tcaatggagg catccagacc tttcaggcga gacagcttcg
     5521 ccaccagcat caacagcaca gttaactgag tggtgaatgc tttagtggac gccacgccga
     5581 tttccgtacc cgcgttggtc atcagcgcca gatcggattc gcgcaccaga gaagaacccg
     5641 gaacgttaca gattgccagc gaaccaaggt aacccagctc tttcgacaga cgcaggcccg
     5701 ccagggtatc cgcggtttcg ccagactgtg acaaggtgat catcaggctg ttacgacgca
     5761 cggcagattt acgatagcgg aattcagagg cgatttcgac gtcgcacgga atacctgcca
     5821 gcgattcaaa ccagtagcgg gaaaccatac cggagttata agaagtacca caggcgagga
     5881 tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt cggtcccagc tcgcttaaat
     5941 caacctgacc gtggctgatg cgcccggtaa gggtgttttt tatcgcgttc ggctgttcgt
     6001 agatctcttt ctgcatgtag tgacggtaaa tacctttatc gcccgcgtca tattgcagat
     6061 tggattcgat atcctgacgt tttacttccg cgccagtttt atcgaagatg tttaccgaac
     6121 ggcgagtgat ttccgcaata tcgccctctt caaggaagat aaagcgacgg gtcaccggca
     6181 acagcgccag ctggtcagaa gcgataaagt tttcgcccat acccaggcca atcaccagcg
     6241 gactaccaga acgtgccgcc agcagggtat ccgggtgacg ggagtccatg atcactgtac
     6301 cgtacgcacc gcgcagctgc gggatagcac gcagaacagc ctcacgcaga gtcccgcctt
     6361 gtttcagctc ccagttcacc agatgggcaa tcacttcggt gtcggtttca gaaacgaagg
     6421 tatagccacg cgcttttaac tcttcacgca gcggttcatg gttttcgatg atgccgttat
     6481 gcaccaccac aatgtgttca gaaacatgcg gatgcgcgtt agcttctgaa ggttcaccgt
     6541 gggtcgccca gcgagtgtga gcaataccgg tgccgccatc cagaggatgt tcttccgctg
     6601 cctgagccag catctggact ttaccgaggc gacgcaggcg ggtcatatga ccttccgcat
     6661 caacaacggc cagaccggca gagtcatatc cgcggtattc cagacgacgt aaaccttcaa
     6721 gaaggatttc tgctacatca cgttgcgcga tcgcgccaac aattccacac atagtttttg
     6781 attccgattt atatcgttgt cggtcaacct gtatgcccgt tttttcgggc gccccgagcc
     6841 ttgtagagag tggggttatt tttatagtta ctgcttgtgg gagggagatt atgttatctc
     6901 ctcatcccag atgaccggat tacccggcca gaattacttt ttctttaccg gacgacgcca
     6961 gccttctttc tgagtctgcg gcacacggct gatagctaat gcattttcgc cgacattacg
     7021 cgtcacagtt gtacccgcag caatggtcgc gcctttgcct actgttaccg gggccaccag
     7081 ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg gtcttaaatt tattcgcacc
     7141 atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg ttatcgccaa tttccgcatc
     7201 gcccaggtaa gtcagatgcc cgactttcga gcctttaccc agacgcgctt ttttcatctc
     7261 aacgaagtta ccaacgtgtg caccttccag caactcagca ccaggacgca aacgagcaaa
     7321 cgggccaata gtacaggccg ctgccagatt cgcgtcttcc acaacggtat acggactgat
     7381 ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa ccggtgccga ttttcacgcg
     7441 atgaccgaga gtcacgttgc cctcgatgat aacgttagta tcaatttcaa catcgcgccc
     7501 gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg cgcagcataa cgcctgctaa
     7561 cagcagtttt tcagcctgtt cggactggta aacgcgctcc agacgggaga gttgcaggcg
     7621 gttattcaca ccttctactt cgcttaaacg ttgcggatga acggcgacga tttcacgccc
     7681 ttcctgatac gccagcgcaa taatgtcggt gatgtagtat tcgccctgag cattattgtt
     7741 ggtcagcttc gccagccagc gtttcatatc tgcgccgtta gcgatcagaa tgccggtgtt
     7801 gatctcctga atctgacgct gctcgtcggt ggcatctttg tgctcaacaa tgccggtaac
     7861 tttgccattt tcacgggtga tacgtccata accggtcgga tcatccagtt tcaccgtcag
     7921 cagaccaatg ccaccctgcg gtttagcatc acgcaggcgc tggagtgttt cgacagagat
     7981 cagcggcacg tcgccgtaga gcattaaaat gtcttcatca tcggcaaaga aaggtgcggc
     8041 ctgctgcatt gcatgacccg tacccagctg ctctgcctga agcacccagt taaggttgtc
     8101 gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg tacaccaagt gaacgtgcgc
     8161 tgcgcctaat tcattcgcag catcaatgac atgctgaacc atcgctttcc cggcaagggt
     8221 atgcagcact ttcggaagat cggaatacat gcgcgtgcct ttgcctgcgg caaggatcac
     8281 tacgctcata gcattattca acatacgcgt cctgactgta atttgagaac gaatttaaac
     8341 cgcttcacct tgaaaaaact acattttttt catcgtgaaa tggacagagg ataaattgtt
     8401 caataacgga ttatccccgc tgacgaaagt gccattttcg accatcgtca cgccttttgt
     8461 ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg tggatgtttt tgctcttatt
     8521 tcgatcaatg aataaacggg aaaacagggg tgttttatat cagcaggatc tatgtgaacg
     8581 ctcttcagga cggatcacgc gtaaaaaaaa gccagcctgt ttccagactg gcttttgtgc
     8641 ttttcaagcc ggtgttacat cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc
     8701 gctttggcca gttccgcaga cgcctgagcg taatctacgt cgccgtgaga gctgctaatg
     8761 tgctcttcag ccttacgttt cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga
     8821 attgcggtgt cggccagaac ggtcacgttg ccaggctgca cttcaagaat gccgccagac
     8881 agatagataa actcttcgtg accgtgctgt ttcacgatgc gaatcatacc aggcttaatg
     8941 gcggtgagca gcggtgcgtg gccagggtag atccccagtt caccttcgct acccgttacc
     9001 tggattttct cgaccagacc agagaacatt tgttgctctg cgctgacgac gtccaggtgg
     9061 taagtcattg ccatatcacc ctccgattaa ggcgttaaag ttttttggct ttttccacag
     9121 cttcttcgat ggaaccgacc atgtagaacg cctgctccgg caggtgatcg tattcgcctt
     9181 ccatgatgcc tttaaagcca cggatggtgt ctttcaggga gacgtattta cccggagaac
     9241 cggtgaatac ttctgccacg aagaacggct gggacaggaa gcgctggatc ttacgagcac
     9301 gcgctaccac cagtttgtct tcttcagaca gttcatccat acccaagatg gcgatgatgt
     9361 ctttcagttc ctgataacgt tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt
     9421 cctgaccaac caccagcggg tccagctgac ggctggtgga gtccagcggg tcaacggccg
     9481 ggtagatacc cagagacgcg atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa
     9541 aggtggttgc cggagacggg tcagtcaagt catccgcagg tacgtatacc gcctgtacgg
     9601 aggtgataga accagttttg gtggaggtga tacgttcctg cagaacgccc atctcttccg
     9661 ccagggtcgg ctgataacct accgctgaag gcatacggcc cagcagtgcg gatacttccg
     9721 taccggccag ggtgtaacga tagatgttgt caacgaacag cagaacgtca cgaccttcgt
     9781 cacggaattt ctcagccatg gtcagaccgg tcagagcaac gcgcagacgg tttcccggcg
     9841 gctcgttcat ctggccatac accagggata ctttgtcgat aacgttggag tcggtcattt
     9901 cgtggtagaa gtcgttaccc tcacgagtac gttcacctac gcccgcaaac acagagtaac
     9961 cggagtgctc gatcgcgatg ttacgaatga gctccatcat gtttacggtt ttacctacac
    10021 ccgcaccacc gaacagacca actttaccgc ccttagcgaa cggacacatc aggtcgataa
    10081 ctttgatacc ggtttccagc agttcctgag agtttgacag ctcttcgtag gaaggtgctg
    10141 cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc tttcatgtcg accggttcac
    10201 ccagtacgtt catgatacgg cccagagtcg ctttacctac cgggacttcg atcgggtgtt
    10261 cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac
    10321 gcacgatacc gccgccgagc tgctgctgaa cttccagcac cagacgctca ttaccatttt
    10381 gcacctcaag agcatcgtac acgcgcggta cggcatcctg agggaattcg acgtcaacta
    10441 cggcgccgat tacctggaca atctttccag tagccatctt aaatcctcta cgaaataacc
    10501 tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg
    10561 acgagctttg ttgtatacca actgcagctc tttaatcagg ctgccgccat tgtcggtcgc
    10621 ggctttcatc gccaccatac gggcggcctg ctcgctggcc aggttttcaa ccacgccctg
    10681 ataaacctga gattcgacat aacgacgcag cagggtatcc agcaacgcct tcggatcggg
    10741 ttcgtacagg taatcccagg atttatgttt cagatcatca tcatctgatg ccggtaacgg
    10801 cagcagctgg ctgatggtcg gaacctgaga catggtgtta ataaatttgt tgctgacaat
    10861 gtaaagcttg tccagacggc cttcgtcgta agcctgcaac atcactttta ccggaccgat
    10921 cagttcggac agggaagggt tatcccccat gccggtgacc tgggcaacaa cattgccgcc
    10981 cacggagttg aagaacgaca cgcctttcga gccgatcatt gcgaggtcgc attgaacgct
    11041 tttgtcggtc caggtcttca tttccgccag tagttttttg aacaggttaa tgttcaaacc
    11101 accgcacaaa ccacggtcgg tcgacaccac caggtagccc acgcgtttaa cgtcgcggtc
    11161 ttccaggtaa gggtgcttat attccagatt accgtgtgca aggtgaccaa tcactttgcg
    11221 catggtttct gcataaggac ggctggccgc catgcgatcc tgcgatttac gcattttgga
    11281 agcggcgacc atctccatcg ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt
    11341 actacgtatc tcttttgcgc cggccatgag cttctcctca atgccttgcg gcctgcccta
    11401 aggcaagccg ccagacgtta ccaggattgg gttgctttga aggaatcgag gatgcctttc
    11461 agcttgcctt cgatttcgtc gttgtagcca ccggtctggt tgatctcttg catcaacgga
    11521 gcgtgatcac ggtcgacgta agccagcaga gcggcttcga agctgccaat tttcgacagt
    11581 tcaacatccg ccaggtaacc acgttctgct gcgaacagaa ccagagactg ctgcgcaacg
    11641 gacatcggcg catactgttt ctgtttcagc agttcggtca ctttctgacc gtggtcaagc
    11701 tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag agaacgctgc cagttcacga
    11761 tactgtgcca gagcggtacg gataccaccg gacagttttt tcatgatctt ggtctgtgct
    11821 gcaccaccaa cacgggatac ggaaataccc gggttaaccg caggacgaat accggcgttg
    11881 aacaggttgg tttccaggaa gatctgacca tcggtaatgg agattacgtt ggtcggaacg
    11941 aacgcagaaa cgtcacccgc ctgagtttcg ataatcggca gtgcggtcag agaaccggtt
    12001 ttccctttca cttcaccttt ggtgaaggct tcaacgtatt cggcgttaac acgtgcagca
    12061 cgctccagca gacgagagtg gaggtagaaa acgtcgcccg ggaatgcttc acgtcctggc
    12121 ggacgacgga gcagcaggga gatctgacgg taagcaacag cctgtttaga caggtcatcg
    12181 taaatgatca gcgcatcttc accgcggtca cggaagtatt cgcccattgc gcaaccggca
    12241 tacggtgcca ggtattgcag tgcagcggat tcagacgcgg ttgctaccac aacgatggtg
    12301 ttagccagtg cgccgtgctc ttccagttta cgtaccacgt tagaaatggt ggacgctttc
    12361 tggccgatag cgacatagat acatttgata ccggaatcgc gctggttgat gatggcatcg
    12421 atagccagtg cggttttacc tgtctgacgg tcaccgatga tcaattcacg ctgaccacga
    12481 ccgattggga tcatggagtc aacggcttta taaccggtct gtaccggctg atctacggac
    12541 tgacgttcga taacgcccgg agcgattgct tctacagcag agaagccgtc gtgatccagc
    12601 ggacctttac cgtcgattgg tgcacccaga gtgttaacca cacggcccag caggccacgg
    12661 ccaaccggaa cttccaggat acggccagta cacttaactt tcatgccttc ggcaaggtca
    12721 gcgtacggac ccataacaac cgcacctaca gagtcgcgct caaggttcag tgcgatagcg
    12781 taacggttac ccggcaggga gatcatttca ccctgcatac aatcggccag gccgtgaatg
    12841 cggataacac cgtcacttac agaaacaata gtaccttcgt tgtgagcttc actcacaaca
    12901 ttgaactgag caatgcgctg cttgatcagt tcgctgattt cggtggaatt cagttgcatg
    12961 ctccagtccc cttaagactg caagacgtct gcaaggcgct caagacgacc gcgtacgctg
    13021 ccatcaatga ccatatcacc cgctcggatg ataacgcctg ccattacaga cttatcgatt
    13081 ttgcaattca gcttaacttt gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg
    13141 agctgttgtt cactcagtgc ggcagcggaa atgacgtcta cctcagcggt agcctcactc
    13201 acggcacgaa ggtgaataaa ctgctccaga acatccggga gcgcgttaag acgaccattt
    13261 tcagccatta cccgaatcag gttctgaccg ttttcgtcca gttgctcacc acaaactgcg
    13321 ataaacgact cggcgagcgt ttctggcgca agcgcgccag agagaagctc tgccatttgt
    13381 tcgtttttgg ttacctcggc ggcaaacgcc agcatgtcct gccagcgttc tacactttgg
    13441 tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc gagctaccgt aataaattca
    13501 gacatcagcc cctccctcct tacagttcag cgacaagttt atccacgatg tcgctgttag
    13561 cagcttcatc cacggaacgt tcgatgatct tctcggcgcc agcaacagcc aggatagcaa
    13621 cttgcttacg cagctcttca cgggcacgtt tacgctcggc ttcaatttct gcctgcgcct
    13681 gggccacgat tttagtacgt tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg
    13741 agcggcgttt gttcgcctgc tcgatgatta cctgggcttc cgctttcgct tttttcagct
    13801 ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa
    13861 ggccgtcagc aatttctttt tgacgttttt cgatggctgc cattaatggc ggccatacgt
    13921 acttcatgca gaacagaacg aacaggacaa acgcgatggc ctggccgagg attgttgcgt
    13981 taagattcac agcacaatgc ctctatttag ttaacgttct gatattgctc tttaaataaa
    14041 agcaacgctt actacgcgac agcgaacatc acgtacagac ccagacctac agcgatcatc
    14101 gggatagcat ccaccagacc cataacgata aagaactgag tacgcagcag aggaatcaga
    14161 tcaggttgac gcgctgcgcc ttccaggaat ttacccccga ggatgccgat accgatcgca
    14221 gcaccgattg ccgccagacc catcatcaca gcggcagcca tgtacagcag atccatattc
    14281 aggttttcca tgacagtctc cagtttgttt cagttaaaac gtagtagtgt tggtaaatta
    14341 atgttcttca gacgccatcg acagatagac gatcgtcaga accatgaaga tgaaggcttg
    14401 cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca ttcaggatcc actgtgacca
    14461 ccacggcaac agaccagcaa tcagaatgaa aatcagctca ccggcataca tgttaccgaa
    14521 cagtcgcaaa ccgagtgaaa ctggtttgga cagcaggctt accccttcaa ggattaagtt
    14581 gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc
    14641 gatgcctttc attttgatgc tgtagaacag aatcaggata aatacgccca gtgccataga
    14701 cagcgttacg ttcacgtccg cagacggaac cacacgcagt gcaggcagac ccagtacatg
    14761 ttcagcaatg tacggcagca ggtcgatagg cagtaaatcc atcaggttca tcaggaatac
    14821 ccagacgaag atcgtcaggg ccagcggagc aatcagcttg cttttgccat ggtacatgtc
    14881 tttcacgcta ccattaacaa agccgatcac cagctcaatc gcggtctgaa acttacctgg
    14941 cacaccgctg gtcgcctttt tggctacgct acggaataaa accaggaaca acagacccag
    15001 caccaccgag aagaacatgg agtcaatatt gattgtccag aaggtggctg gggggttttg
    15061 tggatccacc agcgagaatg tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat
    15121 gtaatcctgc ggcgtcatat tttctgaagc catgatgcct tttacccttt gttgttaatt
    15181 acagccggtg ccagtatctg aaccaccagc accaaaaccc acgtaacgat cagcggcaag
    15241 aataccgcct ttaaaaccgc caacgccacc accagtaaca ccaacatcgc cagaactttg
    15301 aaagcttcgc caaatgcgaa tgtccaggcc acccggcctt tcgctggtgt atacgcctgg
    15361 tgacgccagg caaatatcat aaacaaaacg ttaggcagaa agactgccag gcccccgctt
    15421 attgcagaga cgccccagaa ggggtctttg aggctgaaca gcaatccact tgctatcacc
    15481 accagtaact gaacgagcag aagcttccga gcaacgtttc gactcacgag cgacacagac
    15541 atcacgtttt tcactcctgc tcccttcgag gtatgccgcg tgtcgtataa aactttcttt
    15601 aaggcttaga gtcaagcatc aaaaagcggt caaattatac ggtgcgcccc cgtgatttca
    15661 aacaataagt agccaaaagg tgaataaatg tttaaatatt tttccagtgc atacaattgc
    15721 gacttttccg ctaaccctgt tcgatcatga aaaactgtaa ataacgcgta aacactagtg
    15781 ataaagcgtg cttcagatca catattgcgc atgttcgcgc acagtatatt tatttacttg
    15841 gcaaatgatg cctttgcaag tttatgatat ttcagtctaa aaacagatac tgttttaata
    15901 aatgacattt acacaacaaa aaccacccat tgacattttt aataatgttt taacagccaa
    15961 tgatggttct tagcgccgat ttttagcaga ctgatatttt cactaatgac ttattttctg
    16021 cttaccaaaa aaagccacgt tatcttgttg atacaaaaga gtgaacgtgg cgttaaatgt
    16081 aaccagttat atcagtagaa aacttggttg ttgttaacag tctaaccggt caatttttta
    16141 tgattttttt gataaaaatt aaattttatt tgctttaatc accaccagat gacgttcgcc
    16201 atccagagct ggaacctgaa gtttaaccac tgattcgacc tgatattctt cgggcaacaa
    16261 agcgatttca tcttccggca tttgcccttt cagcgcgtag aaacggcctt gctcaccagg
    16321 aagatggtgg caccagctca ccatatcgtt cagagaggca aaagcgcggc taattacgcc
    16381 atcaaatggc ggctctgaag gaaactcttc taccctgctc tgtactggct caatattctc
    16441 cagtttaagc tcatgttgca cctgacgaag gaaacgcacg cgtttaccaa ggctatccaa
    16501 cagagtgaaa tgggcttcag gacgcacgat agagagtgga atgcctggca gtcctggtcc
    16561 ggtgccgaca tcgataaacc gttcaccttg cagatacggt gccaccacaa tgctatcgag
    16621 aatatggcgt accagcatct cattaggatc gcggaccgaa gtcaggttgt acgctttgtt
    16681 ccatttatgc agcatattca cgtaggcaat aagctggttt ttctggtgat cggtaagcga
    16741 aatacctgcg tctttcagca gtaaggagag tttgttgagc acggtgatta cctgttcttg
    16801 atgcgttgcc tggtaagcgg gtgcttacca ggcattttta atgcgttatg cgctacgacg
    16861 cagcataccc tgttttttca gccacaccag cagaatggag atagccgcag gcgtgacgcc
    16921 agaaatacgc gaagcctggc cgatagaggc cggtttgtga tcgttaagtt tggcgatcac
    16981 ttcgttagaa agaccggata cctggcggta atccagtgtc gcgggtagca gggtgttctc
    17041 gttacgcagc tgcttttcga tctcatcttg ctggcgcgcg atataacctt cgtatttaac
    17101 ctgaatctca acctgttccg ccgcctgttc gtctgtcaac gcaggggcaa acggcgtcag
    17161 cgtggttaat ttttcataag tcatttccgg acgacgcagc agatcttcac cactggcttc
    17221 acgggaaagc ggcgcagtca ggtgagcatt cacttcggct gcagcttccg ccgacggggt
    17281 tacccaggtc gatttcagac gctgacgctc acgctcgata ttctcaagtt tctcgttaaa
    17341 gcgcgcccaa cgttcgtcat ccaccaggcc cagttcacga ccgatttcag tcaaacgcag
    17401 atccgcatta tcttcgcgca gcatcagacg atattctgcg cgcgaagtaa acatacgata
    17461 cggttctttg gttcctaaag tgcacaggtc atcaaccagt acgccgagat acgcctgaga
    17521 acgcgccgga gcccaacctt ctttgtcagc agacagacgg gcagcgttaa gaccgaccag
    17581 caaaccttgc gcagcggctt cttcgtaacc ggtagtgccg ttaatctgac cagcaaagaa
    17641 cagcccctgg ataaacttgc tctccagcgt cggtttcagg tcgcgtggat cgaagaagtc
    17701 atactcaatg gcataacccg gacgcacgat cttcgcgttt tccatcccct gcatggagcg
    17761 gacgatttgc atctgcacat cgaacggcag gctggtggag ataccgttcg gataaatttc
    17821 gttagaggtc agcccttccg gttcaaggaa gatctgatgc tgatttctgt cggcgaagcg
    17881 catgactttg tcttcgatcg atgggcagta gcgtgggccg acaccttcga tcacccctgc
    17941 gtacattggg ctacgatcga ggttactgcg gatcacatca tgggttttct cgttggtatg
    18001 agtgatataa cacggcacct gctggggatg ctgggacgca ttgcccataa acgagaatac
    18061 cggcattggg ttatcgccat gctgttgcgc cagcacacta aagtcgatag ttcgagcatc
    18121 aatacgcggt ggtgtcccgg ttttcagacg accaacgcgc agcggcagtt cacgcaaacg
    18181 gcgagaaagc ggaatggacg gcggatcacc agcacgacca ccgctgtaat tatccagacc
    18241 gatatgaatt ttaccgtcga ggaacgtccc aacggtgagc acgacggctt tggcacggaa
    18301 cttcagtccc atttgggtaa cagcaccgac cacgcgatcg ttttcgacaa taagatcttc
    18361 aaccgcctgc tggaagatca tcaggttcgg ttggttctcc agcgccgtac gtaccgcctg
    18421 acggtagagc acacgatccg cctgagctcg ggtagcgcga accgccggtc ctttgcttgc
    18481 gtttagtatc ctaaactgga tacccgcttg atcgatcgct ttcgccatca gaccgccgag
    18541 tgcatccact tcttttacca gatgtccctt cccaataccg ccgatcgccg ggttgcagct
    18601 catctgcccc agagtgtcga tattgtgtgt caaaagcaga gtctgttgac ccatacgcgc
    18661 cgcggccatc gcggcctcgg tgcctgcatg acccccgcca atgatgatga cgtcaaaagg
    18721 atccggataa aacatggtga ttgcctcgca taacgcggta tgaaaatgga ttgaagcccg
    18781 ggccgtggat tctactcaac tttgtcggct tgagaaagac ctgggatcct gggtattaaa
    18841 aagaagatct atttatttag agatctgttc tattgtgatc tcttattagg atcgcactac
    18901 cctgtggata acaaggatcc ggcttttaag atcaacaacc tggaaaggat cattaactgt
    18961 gaatgatcgg tgatcctgga ccgtataagc tgggatcaga atgaggggtt atacacaact
    19021 caaaaactga acaaccgttg ttccttggat aactaccggt tgatccaagc ttcctgacag
    19081 agttatccac agtagatcgc acgatctgta tacttatttg agtaaattaa tccacgatcc
    19141 cagccattct tctgccggat cttccggaat gtcgtgatca agaatgttga tcttcagtgt
    19201 ttcgcctgtc tgttttgcac cggaattttt gagttctgcc tcgagtttat cgatagcccc
    19261 acaaaaggtg tcatattcac gactaccaat accgattgcg ccaaagcgga ccgcagaaag
    19321 atcgggcttc tgttcctgca atgcttcata gaaaggagaa aggttgtccg gaatatctcc
    19381 ggcaccgtgg gtggagctga taatcagcca gatccccgag gcagataaat cttccaacag
    19441 cggaccgtgc agcgtttcgg tggtaaaacc cgcctcttcc agcttttcag ccaggtgttc
    19501 tgctacatat tcggcaccgc cgagggtgct gccgctgata agagtgatat ctgccataaa
    19561 ccgccacctt tattaagagt ggcgtattgt acgctgtgaa cgcgttggga tctacctgtg
    19621 gaaaagtatg ggattaaaaa agccgattat ggcttgatgg tacgcatgat cgggttttgc
    19681 aggacgatca atgtctcggt ggactgaatt tcatcaattg tttggatctt gttgataagt
    19741 acatgctgga gagcgtcgat cgaacggcac attactttta taaagatgct gtagtggcct
    19801 gttgtgtaat aggcttcagt gacttcagca aggctttcca gctttgccag cgcggaaggg
    19861 tagtctttgg cgctctttaa tataatgccg ataaagcagc ctacgtcata accgagctgc
    19921 ttcgggctga catcaatacg cgccccggta atgatccccg cctgcttcat tttctctact
    19981 cgaacgtgaa tcgtccccgg actgacgcca aattgtttcg ccagttcggc gtaagcggtg
    20041 cgcgcattgc ccattaatgc ttccaggatg ccacggtcca gattgtcgat cagataattt
    20101 tccataggat tttcttatgc agattgatga ttcattctat tttagccttc ttttttaatg
    20161 aatcaaaagt gtgttaggct ttttattgaa tgattattgc atgtttgtcg gtttttgttg
    20221 cttaatcata agcaacagga cgcaggagta taaaaaatga aaaccgctta cattgccaaa
    20281 caacgccaaa ttagcttcgt gaaatctcac ttttctcgcc aactggaaga acgtctgggg
    20341 ctgatcgaag tccaggcgcc gattcttagc cgtgtggggg atggcacgca ggataacttg
    20401 tcgggctgtg aaaaagcggt gcaggtaaaa gtgaaagctc tgcctgatgc ccagttcgaa
    20461 gtggttcatt cactggcgaa gtggaaacgt cagaccttag ggcaacacga cttcagcgcg
    20521 ggcgaagggc tgtacacgca catgaaagcc cttcgccccg atgaagaccg tctttctccg
    20581 ttgcactcgg tctatgttga ccagtgggac tgggaacgcg taatgggcga cggtgagcgt
    20641 caattctcga ctctgaaaag cacggtagag gcgatctggg cgggaattaa agcaaccgaa
    20701 gctgcggtta gcgaagagtt tggcctggca ccgttcctgc cggatcagat ccacttcgta
    20761 cacagccagg agttactgtc tcgttatccg gatctcgatg ccaaagggcg tgagcgggcg
    20821 atagcgaaag atcttggcgc ggtattcctt gtcgggattg gcggcaagct gagcgatggt
    20881 catcgccacg acgtgcgcgc accggattat gatgactgga gcaccccgtc agagctgggt
    20941 catgcgggtc tgaacggcga tattctggtg tggaaccctg tactggaaga tgcgtttgag
    21001 ctttcttcca tggggatccg cgtggatgcc gacacgttga agcatcagtt ggcgctgacc
    21061 ggtgatgaag atcgtctgca gctggaatgg catcaggcgc tgctgcgcgg tgaaatgccg
    21121 cagaccatcg gcggcggtat cggccagtct cgtttgacca tgctgctgct gcaactgccg
    21181 catatcggcc aggttcagtg tggtgtatgg ccagctgctg ttcgcgagaa cgtcccttct
    21241 ctgctgtaat aatttatcgc cgccagcgtc tgagcaggcg gcttcgcatc ccggtatcaa
    21301 agcgccagat atgatcgaaa atgcgcatga tgccgggttt gccgtgtgcc gacatcgcca
    21361 cggcatgaaa gcgatgctga tgtacccgct gcagctcttt cactttactc gtcacgtcgt
    21421 caggcaaccg ctgagcgata aaatcagaaa tcaccaccgc atcggcatca aaccattccc
    21481 tgctttgcaa gcgttccata atggcgcgaa aacaactggc aagatcggtg ccgccacgaa
    21541 actgctggct taaaaaacgg attgcttgtt cgatgccttg ggggcctgaa agctcataac
    21601 ggacgatctc ggtggaaaat agcataatat agcagcgccg gttttcagcg agagcaatgc
    21661 gcatcaaggc caggcagaac gctttcgcac actgttcatt aaagccgccc attgagccgg
    21721 aagtatccac acagacaata aacggcccgc gcggctgttc atcgtaatct ttatgtacca
    21781 ccggacgttc gatcactttt tcacgccacg actcaccgtg caggcgatag gtgagcaact
    21841 gtttttccac cagccgacgg taaaactcat actccagttc cgttatccct agtgtcgcca
    21901 gttctggcgg cagaagacgt aaaatatcat cgctttgttg cagaccatca acctgctcag
    21961 gaaccgtcgc cggttcgcgc accatggtgc ggaaggtttc catctgcgca tcgttgcgcg
    22021 gtattgattt ggcttcccga gaacgcccca gctgctctgc caggcgtttc agttccggct
    22081 gttcgttaag aaattcaccg tatttcacaa tcaactgata gtcgccacgt ttaagctgac
    22141 cggcgctcat atcccacaga cgaccagctg cagtattgtt atctgcgaga atcggttcaa
    22201 gttgtccgct cagcgtcatg cgttcttgaa cttcactcaa cagttgttcg cgttcttctt
    22261 ctaatagctg ttgatttaac gtcgttgctt gcacgatcag acttaaacgc caacgctgga
    22321 gaaaaagcgt gtgtaacgct gaagttattg tgctgttagc atcaaccaac tgccgggctt
    22381 gttctgccca tggggaattc agacgatgca gtaagtccag gatctgtggt agctgcacaa
    22441 taaactgcgg cgtggagagg agctggcttt gctgatagca catcacctct tcggtgagtt
    22501 ccggcgggac tcgggcatct ttcagccgac tgcgcagcgc ctcacgccag cggggaacat
    22561 catcagtgat tgccgccttc aggcgtggga atttttcaaa gaagaccgcc agctgcggtg
    22621 aggccagcag tgcgatgatc atctcttcga tcaatccctc ttcgctgacg gccagcatca
    22681 cattaagcgt atccagcgtt agcattgttg tgcctggtga atctgtgcac cgacatcctg
    22741 taggctggct tcaatgcgac ctaaccagtc gccagggata aacagacatt tttgctgttc
    22801 gctgaataaa gcgtgttgct tgcgccagtc gctttcaagc tcttccagtt gttgttttat
    22861 ttcaccaggc agaccttcag ccgatgaacc gggaagtgcc agcgtactgc cttgtaaact
    22921 tacatcgcga acgacaagat gttgggcgct atcaacttcc agattcagtt tctgggcaaa
    22981 gccgataccg ttcagtttac cgcgaatttc accacctttg ctcagccact gttccagcgc
    23041 gctacgctca aaggagatat gaaccacttc catatcatgc agttttaacg gtttttgcag
    23101 cagtagagtc agagtggaag cagtaacgtt aacaggaagt tgatactgct gacgacggct
    23161 gaaaatgccg cccagacgaa ttaccgttaa ggctgtttta tcgctttgtt gctgctggag
    23221 ttgcaggtga cgttgcacaa tcgcgccaag gcgagtcaac atcccttgct gttgccaggc
    23281 atgaccggtc atcaatacat caatttgttg ttgtatcaaa ttcaggcttt gcgcgtcata
    23341 ccacaggcaa tctttcagca aaatgagatc taccggggca acagcactgc gaccgctaaa
    23401 aaaggcgctg gcctgcaata atcggatcgc ttttttccag cgacgatccg agacataagg
    23461 cgcatccggt aatttatcca gttgcaggcg cagcataaaa atcagctcaa atacatgatc
    23521 gggcagcgta atttcaccaa tctctttctg ccagcgttca tattcttcat ctgtgacctg
    23581 caaggcatca ggaaccggat tgtcgttttc atcctgttga ctggtcagca tggagcggaa
    23641 attcgcttta tcctgcactt tatctaacca cagacgaatc agcatgcggt catataacgc
    23701 ttccagactg ctgtctgctt ccggcagctc gttggaggcc gccaccagca gacgcatcgg
    23761 gattttttct acgtgtgcac cgttgcggaa ctggcgctcg ttaatggcgg tgagcaaggt
    23821 attaagaatt gccgggcccg ctttccagat ctcatccaga aagacaattt ctgcttccgg
    23881 caggtaaccg ctggttaaac gttcatagcg cccttcatct tttagcgcct gaatagaaag
    23941 gggaccaaaa acttcttccg gcgtggagaa gcgggtcatc agatattcaa acgcgcgggc
    24001 attctgaaag gcgaatttta agcgccgggc gatcaaactt ttggcaatac ctggcgggcc
    24061 aaggaggaac acactttcac cacttaatgc cgctaataaa cacaagcgga tggcgtggct
    24121 acgttcataa agcccctttt ccaacgaact gctcaggcgg gaaattcttt ccgctaataa
    24181 atgagggtga gccataatga agtggcgtcc tttcgtcaaa agttctgcgt aaattgcgag
    24241 tatagacgtt tcttgctggt ggctaaaata gtctcaaag
//
LOCUS       DAJTVJ010000061        23953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000061 DAJTVJ010000000
VERSION     DAJTVJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.061"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(38..400)
                     /locus_tag="OO448_002338"
     CDS             complement(38..400)
                     /locus_tag="OO448_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6337531.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="OO448_002339"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="OO448_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6337532.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="OO448_002340"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="OO448_002340"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6337533.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="OO448_002341"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="OO448_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6337534.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="OO448_002342"
     CDS             complement(4827..5036)
                     /locus_tag="OO448_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337535.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="OO448_002343"
     CDS             5321..5683
                     /locus_tag="OO448_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6337536.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6226..6904
                     /locus_tag="OO448_002344"
                     /pseudo
     CDS             6226..6904
                     /locus_tag="OO448_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6948..11798
                     /locus_tag="OO448_002345"
     CDS             6948..11798
                     /locus_tag="OO448_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6337537.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12166..13821
                     /gene="lldP"
                     /locus_tag="OO448_002346"
     CDS             12166..13821
                     /gene="lldP"
                     /locus_tag="OO448_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6337538.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13821..14597
                     /gene="lldR"
                     /locus_tag="OO448_002347"
     CDS             13821..14597
                     /gene="lldR"
                     /locus_tag="OO448_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6337539.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14594..15784
                     /gene="lldD"
                     /locus_tag="OO448_002348"
     CDS             14594..15784
                     /gene="lldD"
                     /locus_tag="OO448_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6337540.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15980..16453
                     /gene="trmL"
                     /locus_tag="OO448_002349"
     CDS             15980..16453
                     /gene="trmL"
                     /locus_tag="OO448_002349"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6337541.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OO448_002350"
     CDS             complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OO448_002350"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6337542.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OO448_002351"
     CDS             complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OO448_002351"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6337543.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OO448_002352"
     CDS             complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OO448_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6337544.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OO448_002353"
     CDS             complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OO448_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6337545.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19349..19780)
                     /locus_tag="OO448_002354"
     CDS             complement(19349..19780)
                     /locus_tag="OO448_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6337546.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20025..21569
                     /gene="gpmM"
                     /locus_tag="OO448_002355"
                     /gene_synonym="pgmI"
     CDS             20025..21569
                     /gene="gpmM"
                     /locus_tag="OO448_002355"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6337547.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21579..22862
                     /gene="envC"
                     /locus_tag="OO448_002356"
     CDS             21579..22862
                     /gene="envC"
                     /locus_tag="OO448_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6337548.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22866..23825
                     /locus_tag="OO448_002357"
     CDS             22866..23825
                     /locus_tag="OO448_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6337549.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6045 a         6108 c         6212 g         5588 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gatttaattg tgaaatttta
     5941 atcatcatat tcatacaata aattatctgt aaattttttc ccagacatgg tttctttcat
     6001 atttgacatt tattaataat catcattaga atccacgaaa ataatatatt catacaaaca
     6061 cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat
     6121 cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata caatggacaa
     6181 ctctaccggc attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag
     6241 atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt
     6301 gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacatta aaattgatct
     6361 acttgaaaaa caacaagcac agctaaagag tcaactggaa actattcaaa aacaacaaac
     6421 tggcataata agcagtacaa agactttaac tcacgtaatt aaatccgtta aagatcaaca
     6481 aaacactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa
     6541 tggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga atggcagtgt
     6601 tattcatatt tcactggtca acttattaag tacacctatc tcaaatattg gttttaatgc
     6661 gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact
     6721 tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa
     6781 tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat
     6841 ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa
     6901 ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat
     6961 ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa
     7021 gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa
     7081 tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggccag ggtgttgatt
     7141 acggtagtgg atcagctggc gacagctggg ttgctatagg caaaggggcg aaagcaaata
     7201 cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc
     7261 aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg
     7321 gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat
     7381 tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg
     7441 ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctggga aatgccacta
     7501 aagctactga gattatgagt attgctcttg gcgacaccgc caatgcgtca aaagcgtatt
     7561 caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta
     7621 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
     7681 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
     7741 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
     7801 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
     7861 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
     7921 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
     7981 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
     8041 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
     8101 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
     8161 tttatgccat tagcgactcg gtagcaaaaa ggcttggagg aggggctgca gtagatgttg
     8221 atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag
     8281 gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat
     8341 acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg
     8401 gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg
     8461 acgatgtcga agccaacacc gccaatatcg ctactaatac cagcaacatt gccacgaata
     8521 cagcaagtat tgccaccaat accaccaata tcaccaacct gacggattcc gttggtgacc
     8581 ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc
     8641 aggataccac cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg
     8701 atgctgttaa cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca
     8761 atatcgccaa taacacttcc aatattgcca ctaacaccac caacatctct aatctgactg
     8821 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca
     8881 cggcagctca tggcaccgag accaccagca aaatcaccaa cgttaaagat ggcgacctga
     8941 cgactggcag caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg
     9001 cgacgaatac caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg
     9061 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag
     9121 ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa
     9181 ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct
     9241 acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa
     9301 tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta
     9361 gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac
     9421 acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact
     9481 ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg
     9541 cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg acgtacacca
     9601 ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat accactcttg
     9661 acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg
     9721 gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca
     9781 gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc gctgatgcgc
     9841 tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact tacaccattg
     9901 cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat aacgccctgc
     9961 tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat gacggtaaag
    10021 ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc gatgcagtga
    10081 acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa attatcaatc
    10141 agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt atcaactacg
    10201 ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt gtcggcgcta
    10261 cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt ggtcagaaca
    10321 gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc agccgtgtga
    10381 ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt ggttatgaca
    10441 ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa taccgccaaa
    10501 tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc cagttgcaga
    10561 acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac tcaacggcag
    10621 aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact gtcgttaatg
    10681 gtaatgcagg tattggtatc ggtttgaaca ctctggttct agctgatgcg atcaacggca
    10741 ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg ggtaacggtt
    10801 ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac gcaccgcaga
    10861 actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc acaaacgtcg
    10921 cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc actgatgagc
    10981 gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact aatctggata
    11041 ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt agcaccaagt
    11101 acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat agcgttgcta
    11161 ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc ggttccgttg
    11221 cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt atcaccaacg
    11281 tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag
    11341 caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc aatatcaccc
    11401 tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc gtcaacaaca
    11461 acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag caatacaccg
    11521 atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg
    11581 gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg ggtgccagca
    11641 tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta ggtgtatcga
    11701 tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat agccagggtg
    11761 aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag ttagtgtgac
    11821 taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata aggcgccccg
    11881 ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac cagacatctc
    11941 cccccacaag aattggccct accaattctt cgcttatctg acctctggtt cacaattccc
    12001 caattaaaac tcacatcaat attgccaata cataacattt agttaaccat tcattgtcat
    12061 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
    12121 caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa
    12181 caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc
    12241 ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
    12301 acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
    12361 ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg gatcattatt
    12421 gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg
    12481 tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc
    12541 ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg
    12601 ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat tgttaacacc
    12661 gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca agtaacaggc
    12721 atcgacagct ttgagattgg ccagatggtg gggcggcagc taccgtttat gaccattatc
    12781 gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaagga gacgtggcct
    12841 gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt
    12901 gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
    12961 ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc gtcaaagatt
    13021 gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc ctggacaccg
    13081 ttcctgttcc tgacagctac cgtaacactg tggagcatcc cgccgtttaa agccctgttc
    13141 gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
    13201 ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag
    13261 tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc
    13321 tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
    13381 gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc
    13441 ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc
    13501 tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc
    13561 ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg
    13621 gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct
    13681 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
    13741 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
    13801 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
    13861 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
    13921 agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag gcgctggcaa
    13981 aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc
    14041 gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg
    14101 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat
    14161 ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag
    14221 caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga
    14281 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
    14341 tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac
    14401 aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg
    14461 cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg
    14521 aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga
    14581 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
    14641 tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg
    14701 ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc
    14761 cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc
    14821 accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc
    14881 ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
    14941 cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt
    15001 tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt
    15061 ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa
    15121 tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
    15181 cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg
    15241 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
    15301 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
    15361 ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg
    15421 tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc
    15481 ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt
    15541 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
    15601 ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa
    15661 agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag attcgctggt
    15721 gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc
    15781 atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga ccgttcggcg
    15841 ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc tcgcccctcc
    15901 ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg ctatcctgcc
    15961 cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa ttccgccaaa
    16021 tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctgcata tcatcgaacc
    16081 gatgggattt gcctgggacg ataagcgcct gcgccgcgcg gggctggact atcacgagtt
    16141 taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa atccccagcg
    16201 cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct atcaggatgg
    16261 cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc ttgatgccct
    16321 gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca tgaatctgtc
    16381 caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt
    16441 gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg cacgatcaca
    16501 ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa atgctggtcc
    16561 atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg aacgccagcg
    16621 gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat cttcgcgccg
    16681 cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac accttcacga
    16741 attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac agattgcaga
    16801 atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg
    16861 agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa
    16921 acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa ccagtgaccg
    16981 atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt tgagtattta
    17041 tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt
    17101 tccgggtcag cggcgtaggc ttcttccacc acttcacgga tagcaatagc aggcataatt
    17161 ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac taccaaggtt ttcgtgcttg
    17221 agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag cgttctggct
    17281 tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc ttactccaca
    17341 cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa caaaggttcc
    17401 ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataacg tcaatgctgc
    17461 ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta ttggcatttc
    17521 tacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc cttccaccac
    17581 ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca tcatgccaaa
    17641 acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca gccccgccat
    17701 gcccataaag gtggcaggat cggcacccag cgccgcgcca agacgcgaca tttcagccag
    17761 cccacgggtg atcagtgccg tacgcgcatt cgcaccaaaa ccgataccgt cggacatccc
    17821 cgcgccaatg gcaatgacgt ttttcaccgc accgccaagc tgcacaccga tgaaatccgg
    17881 gttgctgtaa acgcggaaac ttttgccaca gtgcagcaat tgttggagat catcggcaaa
    17941 ggtctggtcg gtagaggcca acgaaatagc cgtcggtaaa cctgccgcca gttctttcgc
    18001 aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct cacgcgccac
    18061 gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca ccagacgcgc
    18121 atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat ggctgggtac
    18181 gacgacgaga atattacggc tggctgccag cgcagtggcg agatcgcttt caagatggag
    18241 cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt
    18301 tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc ttgccagggt
    18361 aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag cattacgttg
    18421 gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg caaatagttc
    18481 atgaacagcg catcgaagtt aatcggcgca aggttcagtt gcgggaatgt accgcgggat
    18541 accacgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca gtatgcgccc
    18601 aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc gccctgctga
    18661 acttcacaca ggaacgcggt ttcttcgccc agggaggccg ttacggtaac acgcagtacc
    18721 acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag tttaacttct
    18781 ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg
    18841 gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga catgtgtaga
    18901 aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc acacgttatt
    18961 tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccgccaa
    19021 tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt ttgatcatct
    19081 cttcacgctt ggcggcattg ccatcgatcg gcaactcctg aaaactcacg cccttgctgc
    19141 tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat
    19201 tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg gcggctgcaa
    19261 aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt gcagctaacg
    19321 gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc aagttttcgc
    19381 cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca gctttcgtca
    19441 gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata accggtttgt
    19501 ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacagattga
    19561 tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag
    19621 cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg
    19681 tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca
    19741 gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac
    19801 gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc
    19861 gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg
    19921 gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta
    19981 gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa
    20041 aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag caggataacg
    20101 ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc
    20161 tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag
    20221 taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg
    20281 ttgaaatcaa agatcgcgct ttctttgcta atccagtgct gactggcgcg gtagataaag
    20341 cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc ggcgtacaca
    20401 gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa
    20461 tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc
    20521 tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg
    20581 gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc
    20641 tgctgactct ggcgcagggt gagtttcagg ccgatactgc cgttgctggt ttgcaggcag
    20701 cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc
    20761 agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc
    20821 gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc gcgcgtaaga
    20881 aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg
    20941 ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca
    21001 aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg
    21061 gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg
    21121 ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg
    21181 ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag
    21241 gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat cactgcgtgg
    21301 aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg
    21361 gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca cacaccaacc
    21421 tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca ggcggcaaac
    21481 tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga
    21541 ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata
    21601 ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg
    21661 ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac
    21721 tcaaatctat tcaggccgat atcgccacaa aagagcgcgc ggtacgccaa aagcaacaac
    21781 aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc
    21841 gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt gatgagatga
    21901 acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac
    21961 aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt ctcagcggtg
    22021 aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac caggcgcgac
    22081 aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag cgtgctgaac
    22141 tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc caacaggcga
    22201 aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa tcttccatcc
    22261 agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg cgtaacagta
    22321 ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt gaggctcagg
    22381 cagttcgcga caggcagaaa gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa
    22441 gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt caggcattct
    22501 ggccagttcg tggccctacg ctgcatcgct atggcgagca gctacagggt gaattacgct
    22561 ggaaaggaat ggttatcggt gcctctgaag gtactgaagt taaagcaatt gccgacggtc
    22621 gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt gagcacggta
    22681 aaggcgatat gagtctttac ggctataatc aaagcgcgct ggtgagcgtt ggttcgcagg
    22741 ttcgtgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggc agaccttcac
    22801 tctatttcga aattcgccgc cagggtcagg ccgtcaatcc acagccgtgg ttgggaagat
    22861 aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct
    22921 cccctgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgaaca
    22981 acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac
    23041 cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg ttgattcatc
    23101 tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctgcgc ccggagatga
    23161 gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg
    23221 ggatcaataa ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga
    23281 tgcaggcgct ggagcgttat aatctttact tcctcgacag cgtaaccatt ggtaataccc
    23341 aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag gtgttcctcg
    23401 acgattcaca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt gacctagcgc
    23461 gtcgcaacgg atcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgttgc
    23521 aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga
    23581 atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac acaccgcgta
    23641 atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta
    23701 atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc
    23761 agcatcagtg gcaaggctgg ggcaaacagc ctgaagcggc gaagcttaac gctagcgtaa
    23821 attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa
    23881 gcggaaagac tacagtaacg cctgataacc cacctttacg ctgcgcacat tggtaatcag
    23941 gcgcttatac ata
//
LOCUS       DAJTVJ010000062        23494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000062 DAJTVJ010000000
VERSION     DAJTVJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.062"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO448_002358"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO448_002358"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6337550.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="OO448_002359"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="OO448_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6337551.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO448_002360"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO448_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6337552.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO448_002361"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO448_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6337553.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO448_002362"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO448_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6337554.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="OO448_002363"
     CDS             5281..5739
                     /locus_tag="OO448_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6337555.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="OO448_002364"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="OO448_002364"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6337556.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="OO448_002365"
     tRNA            6791..6875
                     /locus_tag="OO448_002365"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6923..7054
                     /locus_tag="OO448_002366"
     ncRNA           6923..7054
                     /ncRNA_class="other"
                     /locus_tag="OO448_002366"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OO448_002367"
     CDS             complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OO448_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6337557.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OO448_002368"
     CDS             complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OO448_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6337558.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OO448_002369"
     CDS             complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OO448_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6337559.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10157..13900)
                     /locus_tag="OO448_002370"
     CDS             complement(10157..13900)
                     /locus_tag="OO448_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6337560.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OO448_002371"
     CDS             complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OO448_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6337561.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16023..17819
                     /gene="narX"
                     /locus_tag="OO448_002372"
     CDS             16023..17819
                     /gene="narX"
                     /locus_tag="OO448_002372"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6337562.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17812..18462
                     /gene="narL"
                     /locus_tag="OO448_002373"
     CDS             17812..18462
                     /gene="narL"
                     /locus_tag="OO448_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6337563.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OO448_002374"
     CDS             complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OO448_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6337564.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20042..20395
                     /gene="ychN"
                     /locus_tag="OO448_002375"
     CDS             20042..20395
                     /gene="ychN"
                     /locus_tag="OO448_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6337565.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OO448_002376"
     CDS             complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OO448_002376"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6337566.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OO448_002377"
     CDS             complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OO448_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6337567.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21792..22892
                     /gene="chaA"
                     /locus_tag="OO448_002378"
     CDS             21792..22892
                     /gene="chaA"
                     /locus_tag="OO448_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6337568.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23295..23402
                     /locus_tag="OO448_002379"
     CDS             23295..23402
                     /locus_tag="OO448_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6337569.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5884 a         6145 c         5665 g         5800 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactactgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 ttagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacggagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgcaga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat ggccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggcctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggaa gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcac agcgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact tttcaaaagt ccgtaaactc
     6901 tcaaacgaat ccgcaattaa atattctgcc caagcgggga aggatgagaa gcttcgacca
     6961 aggttcgact tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag
     7021 cgcgcagcgc tgagtaatcc ttcccccacc accatcgctt ccttaaataa tcaaaacctg
     7081 ttactccgag ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc
     7141 tgtaggtcgg ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa
     7201 ccccgccgaa gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct
     7261 ggtacttacg tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg
     7321 ggaacagcag gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac
     7381 catcgaggtg ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca
     7441 gtttcatcat ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca
     7501 gcgcgcactg gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac
     7561 gcacgcgtgg gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca
     7621 cgccgctggc accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg
     7681 cttcatacat ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa
     7741 taccgatatg gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg
     7801 acgccgcgcg ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga
     7861 ccgccccggc tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg
     7921 ctgtcctccg gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg
     7981 atgagcagtg attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc
     8041 ttcccagaca gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc
     8101 cgccactttg tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac
     8161 ggcataacgg ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat
     8221 atctttcaaa ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag
     8281 cggcagatgg tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc
     8341 cagcaggtcc accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag
     8401 caacagtgac gtggcgcgac cacggtcgaa cagttcgctg tactgggcct gggcatcgag
     8461 cggatccatc gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga
     8521 cagatttttc gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc
     8581 atccggatat tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt
     8641 ttgctggtca catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct
     8701 gaaccgtggc agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc
     8761 cgtgccagtt cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc
     8821 agataacggt acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg
     8881 gtatcaactt taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt
     8941 ttcagtgcgc gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt
     9001 accggaatac gcaagctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg
     9061 tctgctgcag actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga
     9121 tattctggat gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac
     9181 tgctgtgcgg cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact
     9241 ttcggatcgt tcggatcgag gaacacctcc agctgacgct ggtaaagatc tttctcgttc
     9301 tcggtgctgg ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg
     9361 atacgaccga cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag
     9421 cagaagatgc acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg
     9481 catccggtga tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca
     9541 tcttcttcac gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg
     9601 cagtgttcgc acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc
     9661 gccttctgga tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg
     9721 tcatcttccc agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa
     9781 cgcggacggg caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgatag
     9841 tcaaaatcga acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt
     9901 ttacccagca acatggcacg gttacccatg cgcggctgca gtttgccgtt gattttacgg
     9961 atccagccgc ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc
    10021 ttggtttcca cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt
    10081 ttacaggtaa ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg
    10141 acttgtgaac gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta
    10201 accagtcaat gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg
    10261 tgccgtagta gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt
    10321 tcggcgtgat acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac
    10381 caggcaggtt aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac
    10441 gctggctgac aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt
    10501 cgttatcggc gatacccaga tctttggcat cggcttcact caaccagacc accggaccac
    10561 cgcgacctaa agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct
    10621 ggtgcggcgt caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca
    10681 cttctttcac cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat
    10741 cacgcatcca ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga
    10801 tcagctcgtg aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag
    10861 accaggtcgg gctggagata attttgcgcg gctgtgcctg aatatcgagg aagcggatct
    10921 tctcgtcttc tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg
    10981 cagcccaggc tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct
    11041 ctgccgcatc aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg
    11101 tgtagttgag cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac
    11161 cgccattacc gattttctcc atcaacgggc caatagaggt aaagcgttcg taagtcgccg
    11221 gataatcgcg ctctacgacc ataatgtgtg gcgcggtttt acctgggatc aggtcgcact
    11281 cgcctttttt ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct
    11341 ggataggcag cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga
    11401 atttcttcgc gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga
    11461 ccgccgcaga cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt
    11521 cgtaccaagt cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga
    11581 agtccagcgt aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt
    11641 cttccggctt cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc
    11701 ccagcaggta cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac
    11761 gccagatgaa caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac
    11821 ggatggaacc ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt
    11881 ccgcttcgcc agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc
    11941 ccatgcgttc cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg
    12001 ccatcggtga cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt
    12061 tatagaaata agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg
    12121 ccagcggctg ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc
    12181 cgcccccgct ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt
    12241 tcatatcgag gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt
    12301 gcgttttatc agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa
    12361 cgccggtaat ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg
    12421 cacagttaac gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa
    12481 cggtgctcac cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt
    12541 gcagcagcac gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt
    12601 acgggaagcc tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg
    12661 tttcttcgcc agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc
    12721 ggaagccaat agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt
    12781 tccattccgg attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac
    12841 gacctgcagc gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt
    12901 agcgacgcac atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca
    12961 ttacgtggcc catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca
    13021 gatcgcacag tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt
    13081 tgtaacgcac ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac
    13141 cccaggcgat gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct
    13201 cgccccaggt ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc
    13261 aagtaccgcc aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg
    13321 ccggaattgg cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt
    13381 tagatgcggc gatcagttcg ttcacctcct gccaggaaga acgaacaaat ccaccgcgtc
    13441 cacgcgcctg cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct
    13501 caaccggatc gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca
    13561 tcatcgggta tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg
    13621 ggcagccgcg aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct
    13681 gggtttccca ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc
    13741 agtttacccc gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat
    13801 cctcccagtc acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct
    13861 tctgtttgaa gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc
    13921 ctgtcggccc tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa
    13981 cagcgtggga attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa
    14041 gatacccctt taggataatt aacagccacc acataacaga ctgtaaatta agacgtttta
    14101 tacctgtagt tatttaaact attaagattt ttatgcagat gggtattaag gagtattccc
    14161 catgggtaac atattaatgg ctcttaatga aatccggcga aataagccat ataatatgag
    14221 tatgttgtaa ctaaatgacg cataaaaaaa gcgcgacatc atgccgcgca aaggataatc
    14281 aaatattaag atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca
    14341 ggcgatatag aaaatcaaaa ataccttcat tgcgccgact ggcgaacccg ttaatgccag
    14401 cgagctacca aacgctttcg ggataaagaa gccaccaata gcgccaatcg cagagatgaa
    14461 acccagcgcc gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc
    14521 ttctgctttc acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt
    14581 ggaaccacta cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat
    14641 gaagcttccg ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc
    14701 catcagaata aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc
    14761 acctgcagaa cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg
    14821 aacatccggg aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgataaagga
    14881 gccgaaggtt gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg
    14941 caactgctcc ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc
    15001 aatggtgaag atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc
    15061 agtcccatcc ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac
    15121 caccagcgga gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag
    15181 acccagcgcg ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga
    15241 tgcgaagttc gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg
    15301 cgtggaggta tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt
    15361 gctgaacgcc gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg
    15421 taataacgcg ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa
    15481 attaaaaccg actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa
    15541 cgccagcagc agacagggaa cggaaatcca caggttacgg ctggcaatac gttgaccgcg
    15601 ttgttgccag aacgcaggat cttccggtcg ccaatctgta atgacagctc cagtagccct
    15661 ttcgggggcg gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct
    15721 tagtgtctgt agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc
    15781 attgatattt atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt
    15841 cacggtgctg taaaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa
    15901 ggagtaactc tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc
    15961 agaatgtatc gtcacattca ttaaggttat tgctcattta aagcctgaag gaagaggttt
    16021 gcatgcttaa acgttgtctc tctccgctca ccctggttaa tcaggttgcg cttattgtgt
    16081 tgctttctac tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg
    16141 ttcagggcag cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc
    16201 tgttggcggc agtgccatta agtgagaaag acaagccctt aattaaagag atggaacaaa
    16261 cggcatttag cgccgagtta actcgagcag cagaacgaga cgaacaactg gcgcaattac
    16321 agggtttaca agattactgg cgtaatgaac tgatccctgc actgatgcgt gcacaaaacc
    16381 gcgaaacggt gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg
    16441 gttttgaccg caccacggaa atgcgcattg agacagtggt actggtccat cgggtaatgg
    16501 cggtatttat ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac
    16561 aaccgtggcg gcaactgctg gcaatggcga gtgccgtcag tcatcgcgat tttacccaac
    16621 gcgcgaacat cagcgggcgc aacgaaatgg cgatgcttgg aactgcattg aacaatatgt
    16681 ctgcagaact ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg
    16741 ggctggagca taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt
    16801 cccgcgcccc gctgtgtgaa cgcctgtcac ctgtactcaa cggcttacag aatttaaccc
    16861 tgctacgtga tatcgaattg cgggtgtatg acactgatga tgaagagaat catcaggagt
    16921 ttacctgcca gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg
    16981 tattacccgt tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc
    17041 agtacggtat tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac
    17101 aactgctgga taccctggtt gaacaactca ccgccacgct ggcgctggat cgccatcagg
    17161 aacgtcagca acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg
    17221 attctattgc ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg
    17281 gcgatgcgct gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg
    17341 catcctgggc gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg
    17401 gattacgtcc ggcgctggag gcgagttgcg aagagtacag cgccaaattt ggcttcccgg
    17461 tgaagctgga ttatcaattg ccgcctcgcc tggtgccttc gcatcaggca atccacttgt
    17521 tgcaaattgc ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaagtcg
    17581 tggtgacggt ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg
    17641 gcgtgcctga aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg
    17701 cgcaaagttt acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg
    17761 tggtcacctt tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat
    17821 caggaaccgg ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa
    17881 cagcttatca gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag
    17941 ggtattgaac tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc
    18001 ggcatgaacg gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt
    18061 gtggtattca gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg
    18121 gatggttatc tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca
    18181 gctgctggcg aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc
    18241 gctaaccgtg ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt
    18301 ctcaagctga ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc
    18361 gaaagcacag taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc
    18421 gtggaagcag cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag
    18481 ttcgtcgttt gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga
    18541 gacacgctgc ccctggttat cttccaccac caccgacaat cgccagtgat tgctcgcccc
    18601 ttccccgttc tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc
    18661 gcccggcgtc aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta
    18721 acgactgcgg atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg
    18781 tgtcgccaga aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt
    18841 tcgctgcgga ttatcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc
    18901 agaaagttgt tttttgagcg gcacaccaaa gcggtaatta agattcagtc cgaggttatt
    18961 ttgattttcg ccactttcac cctgtttatg ctgggccgtc acagtgacta atggcacagg
    19021 ggtgtaattt aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa
    19081 caaatcaaca cgctcaccaa aatactgttc taccctgaca ctggtattga ggtgttgata
    19141 gaacggcatg cgcatccggg ctgtcaggtc atacccgcgc gccatccgtt gttcctgcgt
    19201 ggctgtctgt tcatgccatg cagcaaacgg ctgataaaag tttgccgata gtcgcaaata
    19261 ttcgccccac gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc
    19321 ataaaaagtg ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac
    19381 attgctcacc aacccatcat cctgctgagt aagaccaagc tggctccagg tgagataacg
    19441 atcattatct tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt
    19501 atccactttg acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg
    19561 attaacctgt tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc
    19621 gccagtatcc agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc
    19681 aaagtgtttt tccccatcat ggttttcggg agccatgcct aaatccggca gaccatcatt
    19741 gttattatca aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc
    19801 gcctgccgcc agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac
    19861 gtcggtaact tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa
    19921 cttttgtcgt attttctcgc gctttcagga aaatccttaa aaaggtgacc gattggtcag
    19981 acgctgcaga agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat
    20041 aatgcaaaaa atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa
    20101 cagcttgcgg ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt
    20161 cctgatgtct gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa
    20221 cattcagcaa atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac
    20281 ctgtaccgac gggcgcggga ttagcacact tcctcttatt gatggggtgg aaatcggtac
    20341 tcttgtggaa ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt
    20401 gagggggcgt ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccgtaac
    20461 acaccatccg gataattctc cgccagcagt ttttttaccg ataccagcaa atcattcagc
    20521 ccatcgtcct gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgcg
    20581 ttggttccca gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg
    20641 tcagattcat attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt
    20701 ccatcatcaa gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc
    20761 ttccacaaca gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct
    20821 gtggtgcgtc cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc
    20881 ccggcggtca ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcataat
    20941 tcggtaaact ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt
    21001 ccctcattag gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac
    21061 cataagagtg attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa
    21121 tcacgcgtta tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg
    21181 tcttccggac atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg
    21241 gaccacttgc aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt
    21301 tttatgccat ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac
    21361 tttatgcgcg gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg
    21421 atcccatgcg ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg
    21481 ctttacgctt tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa
    21541 agttgaacat taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg
    21601 tcagactgtt cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat
    21661 gccccaatga gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca
    21721 gacactttct gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag
    21781 ggataaccat aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc
    21841 ttattttccc ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc cagacactac
    21901 cagtggtcat tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg
    21961 ttgtccgtca tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc
    22021 ttagcctttc agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg
    22081 acgccgcgcc aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg
    22141 ggctggttgg cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc
    22201 tgtttggtat caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat
    22261 ttccaatggc tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa
    22321 tttctgcggc aatgtatggc gtatttttgc tgatccagac caaaacgcat caaagtttgt
    22381 ttgtctacga gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg
    22441 cccatagcag cgtgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag
    22501 tcaccaaaat gaatgccagc ccgctggaga cattgctcga cagcatgaat gcccctgtcg
    22561 cctttactgg cttcctggtg gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa
    22621 aagcagtgtt gaacaaccag gtccagcgcg caatgaatct gttctttggt tcagtgttag
    22681 caaccatttc gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat
    22741 tacagtttgc acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc
    22801 atatctcctt ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt
    22861 ttgccgccta tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat
    22921 cgggggagat aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt
    22981 taatcagaat tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa
    23041 agccggaatg tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg
    23101 tagtatccac accaagccgg aattatccgg tgaggcgcaa tgttgcgggg gctttatccc
    23161 tggtggcatt ggttgctgga aagagaaaac ccccgcacgt tgagaggtaa aacctgacaa
    23221 caaaacgggg gctaatcttg aaaccagaca cttcaagaat agccgccttg ttgtcattac
    23281 aacaaggcgg ctatatgacg ctcgcgcagt ttgccatgac tttctggcac gacctggcag
    23341 caccgatcct ggcggggatt attaccgcag cgattgtcag ctggtggcgt aaccggaagt
    23401 aaggtgaaaa cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg
    23461 ggtaacagcc tgcggccatt tttgtcttta tctc
//
LOCUS       DAJTVJ010000063        23235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000063 DAJTVJ010000000
VERSION     DAJTVJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.063"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="OO448_002380"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="OO448_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6337570.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="OO448_002381"
     CDS             507..1136
                     /locus_tag="OO448_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCR6337571.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="OO448_002382"
     CDS             1163..2824
                     /locus_tag="OO448_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR6337572.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="OO448_002383"
     CDS             3063..3122
                     /locus_tag="OO448_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6337573.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="OO448_002384"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="OO448_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6337574.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OO448_002385"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="OO448_002385"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6337575.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OO448_002386"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="OO448_002386"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6337576.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OO448_002387"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="OO448_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6337577.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="OO448_002388"
     CDS             9505..10125
                     /locus_tag="OO448_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6337578.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="OO448_002389"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="OO448_002389"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6337579.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OO448_002390"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="OO448_002390"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6337580.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OO448_002391"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="OO448_002391"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6337581.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="OO448_002392"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="OO448_002392"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6337582.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OO448_002393"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="OO448_002393"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="OO448_002394"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="OO448_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR6337583.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="OO448_002395"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="OO448_002395"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6337584.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="OO448_002396"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="OO448_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6337585.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OO448_002397"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="OO448_002397"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6337586.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="OO448_002398"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="OO448_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6337587.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="OO448_002399"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="OO448_002399"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6337588.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="OO448_002400"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="OO448_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6337589.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5653 a         6331 c         5906 g         5345 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc atgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac ctttt
//
LOCUS       DAJTVJ010000064        23158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000064 DAJTVJ010000000
VERSION     DAJTVJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.064"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            230..1663
                     /gene="cscA"
                     /locus_tag="OO448_002401"
     CDS             230..1663
                     /gene="cscA"
                     /locus_tag="OO448_002401"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6337590.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1671..2729)
                     /locus_tag="OO448_002402"
     CDS             complement(1671..2729)
                     /locus_tag="OO448_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6337591.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2897..3115
                     /locus_tag="OO448_002403"
                     /pseudo
     CDS             2897..3115
                     /locus_tag="OO448_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3133..4461
                     /gene="dsdA"
                     /locus_tag="OO448_002404"
     CDS             3133..4461
                     /gene="dsdA"
                     /locus_tag="OO448_002404"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6337592.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="OO448_002405"
     CDS             complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="OO448_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6337593.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="OO448_002406"
     CDS             complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="OO448_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6337594.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7686..8300
                     /gene="evgA"
                     /locus_tag="OO448_002407"
     CDS             7686..8300
                     /gene="evgA"
                     /locus_tag="OO448_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6337595.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8305..11898
                     /gene="evgS"
                     /locus_tag="OO448_002408"
     CDS             8305..11898
                     /gene="evgS"
                     /locus_tag="OO448_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6337596.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="OO448_002409"
     CDS             complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="OO448_002409"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6337597.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="OO448_002410"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="OO448_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6337598.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="OO448_002411"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="OO448_002411"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6337599.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="OO448_002412"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="OO448_002412"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6337600.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17698..18321)
                     /locus_tag="OO448_002413"
     CDS             complement(17698..18321)
                     /locus_tag="OO448_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6337601.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18618..18893
                     /gene="ypdI"
                     /locus_tag="OO448_002414"
     CDS             18618..18893
                     /gene="ypdI"
                     /locus_tag="OO448_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCR6337602.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18970..19212)
                     /locus_tag="OO448_002415"
     CDS             complement(18970..19212)
                     /locus_tag="OO448_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6337603.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19565..20485
                     /gene="lpxP"
                     /locus_tag="OO448_002416"
     CDS             19565..20485
                     /gene="lpxP"
                     /locus_tag="OO448_002416"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6337604.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO448_002417"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO448_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6337605.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO448_002418"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO448_002418"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6337606.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..>23158
                     /gene="ypdA"
                     /locus_tag="OO448_002419"
     CDS             22592..>23158
                     /gene="ypdA"
                     /locus_tag="OO448_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6337607.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVL"
BASE COUNT         6485 a         5420 c         5044 g         6209 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggacta tatttttata
      181 aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc
      241 tcgattgcat gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt
      301 ctatccccat tttcacctcg cgccacctgc cggatggatg aacgatccaa acggcctgat
      361 ctggtttaac gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg
      421 accaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat
      481 tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga
      541 tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatt gtgcaggtaa
      601 tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga
      661 gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt
      721 gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg
      781 gcagatcctg ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc
      841 ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga
      901 tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa
      961 tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc
     1021 cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc
     1081 gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc
     1141 aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg
     1201 caaacttcta caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt
     1261 ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat
     1321 tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac
     1381 tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc
     1441 acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct
     1501 aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat
     1561 gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg
     1621 agtggctgtg ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa
     1681 caacggatca acagcggaca agggatccgc gtcactcttc ccccttcacg accttcaata
     1741 atatgcaatg cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc
     1801 ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc
     1861 gggatacgta cccccttcgc caggagaacc tgataagcga caaaggctgc acgatcgtta
     1921 ccacatatca gaacatcaaa atctggtttg cccgatttga agtgggcatt gagtaaactt
     1981 gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga
     2041 tctcgtccag catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac
     2101 gcactttccg gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt
     2161 gttgattcat attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc
     2221 gccacacagt tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt
     2281 cgcagcccca ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc
     2341 gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg
     2401 ctggcggttt gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc
     2461 tgcgccagca cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa
     2521 agatcaggaa cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga
     2581 cgcacagatt ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg
     2641 cgtgcgacat cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag
     2701 cagaaacaga cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa
     2761 aaaataacaa taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt
     2821 aaccatatag gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     2881 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     2941 tgctatcggt tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctagt
     3001 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     3061 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     3121 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     3181 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     3241 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     3301 tcccgttttg caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt
     3361 attgaatcag aactggttgc cattccagct atgcaaaaac ggctggaaaa agaatatcag
     3421 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     3481 ataaaagcac gcggcgggat ttatgaagtc ctggcacacg cagaaaaact ggctctggaa
     3541 gcggggttgc tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag
     3601 ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     3661 attatgagcg cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca
     3721 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     3781 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     3841 gatgacgaaa attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa
     3901 gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg
     3961 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc
     4021 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     4081 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     4141 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     4201 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     4261 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     4321 gtatgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     4381 accactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
     4441 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     4501 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
     4561 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     4621 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     4681 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accataatat
     4741 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
     4801 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     4861 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     4921 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     4981 ccctgaaaaa actgcggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     5041 gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcactaa caaccgcatg
     5101 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     5161 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     5221 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     5281 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     5341 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     5401 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     5461 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     5521 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     5581 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     5641 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggc
     5701 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaacccc
     5761 tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca cattaatgaa
     5821 gacagcgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat tctttgtgcc
     5881 aacctgccag taacagggat cgcaatggca tttgctacac caaacgaggt gataacccag
     5941 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     6001 atagtggaat ccaacatttg cataaatgtc gctaatgaca atgcaatagt gacgcaccat
     6061 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     6121 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     6181 tactggtata agccggcatg gaggtcacgg ttgaagccag ctcaggcatc tcagcaatgt
     6241 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     6301 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     6361 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     6421 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     6481 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     6541 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
     6601 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcgcca
     6661 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     6721 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     6781 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     6841 tcgctaaagg cacacgacgg ttataatctt ctaaggattg ttgatactga atacgtgctg
     6901 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     6961 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     7021 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     7081 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     7141 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     7201 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     7261 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     7321 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     7381 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     7441 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     7501 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     7561 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     7621 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     7681 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     7741 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     7801 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     7861 acggtatcct ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     7921 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     7981 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     8041 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     8101 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     8161 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacttata
     8221 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcac
     8281 aacgtaacaa aatcggctaa ccacatgaag tttttaccct atatttttct tctctgttgt
     8341 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     8401 agtaacaacc gtgtcatact tgatccagta cgtctgagca acaaggaatt acgttggtta
     8461 gcgagcaaaa aaaatcttgt gattgcagta cataagtccc aaacggctac gttgttgcat
     8521 accgattcgc agcaacgagt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     8581 acgttaaata tcaaattaac actccgggaa tacgccgatc atcaaaaagc aatggacgcg
     8641 ctggaagaag gtgaagtcga tatagtgtta tcacatttag tagcttcgcc gcctcttaat
     8701 gatgacattg ctgcaaccaa accactgata attacctttc cggcgctggt aaccaccctt
     8761 cacgattcaa tgcgaccgct tacctcatca aaaccagtaa atattgctcg agtagcaaat
     8821 tatccccctg acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     8881 aatttatatc aggcattagc atccgtctca gccggacaga atgattactt tattggtagt
     8941 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     9001 tattataact caccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcgttctt
     9061 aatgaagtac tcaatcgatt tgttgatgct ttgacaaatg aagttcgcta tgaagtatca
     9121 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     9181 catgaaaaac aatggattaa gcagcatccc gatttaaagg tgctggaaaa tccttactcc
     9241 cctccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggatattctt
     9301 aatattatta ctttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     9361 catgctggaa cacagctgaa tcccggcgga tgggatatat tacccggtgc tatttatagt
     9421 gaagatagag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     9481 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     9541 ccatattatt atgagcttca ttcgcaatta aaagagatgt atccggaggt agagtggata
     9601 aaagtcgata acgccagcgc tgcatttcac aaggtcaagg aaggcgaact tgatgctctg
     9661 gtcgcgacac agttaaattc acgttacatg atcgaccatt actatcctaa tgaactttat
     9721 cattttctta ttcccggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     9781 ccggaactta aggatattat taataaagca ctgaatgcaa tccccccaag cgaagttctg
     9841 cgcctgaccg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctctat
     9901 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     9961 tggggatttt acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
    10021 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
    10081 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
    10141 tactacaaaa atgcaatgtt accattagaa aacagtgact ccccctttaa agatgttttt
    10201 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggtt
    10261 tttgaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
    10321 ctgccagcca gcgatcatgc tgtttatatt tgtggttggc aagatattac agagacgcgt
    10381 gatttaattc atgcactcga agtagagaga aataaagcga tcaatgcaac tgtcgcaaaa
    10441 agtcagtttc tggcaacaat gagtcacgaa ataagaacac caataagctc cattatgggc
    10501 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
    10561 cttgcctact ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
    10621 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
    10681 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaatttgc
    10741 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
    10801 ttatcaaatt tactgagcaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
    10861 acctccctgg ttcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
    10921 ggaagtggat tatcgcagga agaacaacaa caactcttta aacgctacag ccaaacaagt
    10981 gcaggtcgcc agcaaacagg atctggctta ggcttaatga tctgcaaaga attaattaaa
    11041 aatatgcagg gcgatttgtc attagaaagt catccaggca taggaacaac atttacgatc
    11101 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacacccc
    11161 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
    11221 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
    11281 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
    11341 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
    11401 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
    11461 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
    11521 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
    11581 aataatacgg cgaacgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
    11641 acgcataaag atctacccgc tgcgtttcaa gcactagaag ctggcgataa cagaactttc
    11701 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
    11761 aatattagcc atcagttaga aataacacct gtttcagatg acagtaagcc tgaaattctt
    11821 cagttgctga actctgtaaa agaacacatt gcagagctgg accaggagat tgctgttttc
    11881 tgtcagaaaa atgactaaat agcggctccc acaatgttct aatgtgggag ctatttacca
    11941 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
    12001 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
    12061 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
    12121 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
    12181 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
    12241 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
    12301 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
    12361 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
    12421 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
    12481 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
    12541 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
    12601 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
    12661 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
    12721 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attacaaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgctgc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggtcgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagcggct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aagagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgcagca gggtcgatga ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgaa tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tatttattcg
    17401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    17461 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    17521 taaatgattt gtgatgacac tggattattg cggtttactt tgaggaaaat aaaatgcgtg
    17581 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    17641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    17701 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    17761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    17821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    17881 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    17941 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    18001 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    18061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    18121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    18181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    18241 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    18301 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    18361 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    18421 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgct
    18481 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    18541 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    18601 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    18661 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    18721 ataaagagac tgaaaaaaca tcagcgatga caaccacaca ctctacaaaa cttcagccat
    18781 cacaggcgat tttgtttaag atgagagaag atgcgccacc attaaacctc acagaagaaa
    18841 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    18901 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    18961 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    19021 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    19081 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    19141 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    19201 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    19261 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    19321 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    19381 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    19441 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    19501 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    19561 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    19621 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    19681 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    19741 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    19801 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    19861 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ctaaacgcgc
    19921 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    19981 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    20041 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    20101 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    20161 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    20221 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    20281 aacgatggta agaaaagcgg attacagcgg atatcgtttg tttatcaccc cagagatgga
    20341 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
    20401 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    20461 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    20521 cataataacc tcatggtttt tatggctttt ttcttaaggt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattaa cgccgtcttg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacggcg
    21301 gcgtttgtac tgttcggtaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atccatggct ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cgacgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cagtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgctt
//
LOCUS       DAJTVJ010000065        22249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000065 DAJTVJ010000000
VERSION     DAJTVJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.065"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..998
                     /locus_tag="OO448_002420"
     CDS             <1..998
                     /locus_tag="OO448_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6337608.1"
                     /translation="VDGLHYFSDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQI
                     QGNTSEDNKENSWTRVAFAGLKFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGS
                     DNFMQQRGNGFATYRNTDFFGLVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDG
                     VGGSITYDYEGFGIGGAISSSKRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQ
                     YTQTYNATRVGSLGWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDD
                     EDILKYVDVGATYYFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1110..2165
                     /gene="apbE"
                     /locus_tag="OO448_002421"
     CDS             1110..2165
                     /gene="apbE"
                     /locus_tag="OO448_002421"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6337609.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2239..3303
                     /gene="ada"
                     /locus_tag="OO448_002422"
     CDS             2239..3303
                     /gene="ada"
                     /locus_tag="OO448_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6337610.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3303..3953
                     /gene="alkB"
                     /locus_tag="OO448_002423"
     CDS             3303..3953
                     /gene="alkB"
                     /locus_tag="OO448_002423"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6337611.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4029..5672
                     /gene="yojI"
                     /locus_tag="OO448_002424"
     CDS             4029..5672
                     /gene="yojI"
                     /locus_tag="OO448_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6337612.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            5890..7536
                     /gene="mqo"
                     /locus_tag="OO448_002425"
     CDS             5890..7536
                     /gene="mqo"
                     /locus_tag="OO448_002425"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR6337613.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7845..8333)
                     /gene="eco"
                     /locus_tag="OO448_002426"
     CDS             complement(7845..8333)
                     /gene="eco"
                     /locus_tag="OO448_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6337614.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(8468..8632)
                     /locus_tag="OO448_002427"
     CDS             complement(8468..8632)
                     /locus_tag="OO448_002427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337615.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            8741..9235
                     /gene="napF"
                     /locus_tag="OO448_002428"
     CDS             8741..9235
                     /gene="napF"
                     /locus_tag="OO448_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6337616.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECCFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9225..9488
                     /gene="napD"
                     /locus_tag="OO448_002429"
     CDS             9225..9488
                     /gene="napD"
                     /locus_tag="OO448_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6337617.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9485..11971
                     /gene="napA"
                     /locus_tag="OO448_002430"
     CDS             9485..11971
                     /gene="napA"
                     /locus_tag="OO448_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6337618.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            11978..12673
                     /gene="napG"
                     /locus_tag="OO448_002431"
     CDS             11978..12673
                     /gene="napG"
                     /locus_tag="OO448_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6337619.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            12660..13523
                     /gene="napH"
                     /locus_tag="OO448_002432"
     CDS             12660..13523
                     /gene="napH"
                     /locus_tag="OO448_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6337620.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13520..13969
                     /gene="napB"
                     /locus_tag="OO448_002433"
     CDS             13520..13969
                     /gene="napB"
                     /locus_tag="OO448_002433"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6337621.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            13979..14581
                     /gene="napC"
                     /locus_tag="OO448_002434"
     CDS             13979..14581
                     /gene="napC"
                     /locus_tag="OO448_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6337622.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14600..15217
                     /gene="ccmA"
                     /locus_tag="OO448_002435"
     CDS             14600..15217
                     /gene="ccmA"
                     /locus_tag="OO448_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6337623.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15214..15876
                     /gene="ccmB"
                     /locus_tag="OO448_002436"
     CDS             15214..15876
                     /gene="ccmB"
                     /locus_tag="OO448_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6337624.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            15918..16655
                     /gene="ccmC"
                     /locus_tag="OO448_002437"
     CDS             15918..16655
                     /gene="ccmC"
                     /locus_tag="OO448_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6337625.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16652..16861
                     /gene="ccmD"
                     /locus_tag="OO448_002438"
     CDS             16652..16861
                     /gene="ccmD"
                     /locus_tag="OO448_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6337626.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            16858..17337
                     /gene="ccmE"
                     /locus_tag="OO448_002439"
     CDS             16858..17337
                     /gene="ccmE"
                     /locus_tag="OO448_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6337627.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17334..19277
                     /gene="ccmF"
                     /locus_tag="OO448_002440"
     CDS             17334..19277
                     /gene="ccmF"
                     /locus_tag="OO448_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6337628.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19274..19831
                     /gene="dsbE"
                     /locus_tag="OO448_002441"
     CDS             19274..19831
                     /gene="dsbE"
                     /locus_tag="OO448_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6337629.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            19828..20880
                     /gene="ccmH"
                     /locus_tag="OO448_002442"
     CDS             19828..20880
                     /gene="ccmH"
                     /locus_tag="OO448_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6337630.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(20915..21562)
                     /gene="narP"
                     /locus_tag="OO448_002443"
     CDS             complement(20915..21562)
                     /gene="narP"
                     /locus_tag="OO448_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6337631.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            21882..>22249
                     /locus_tag="OO448_002444"
     CDS             21882..>22249
                     /locus_tag="OO448_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6337632.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         4946 a         5609 c         6355 g         5339 t
ORIGIN      
        1 aagtagacgg cctgcactat ttctctgaca acaagtcaga agacggcgac cagacctatg
       61 tacgtcttgg tttcaaaggc gtaactcagg ttactgacca gctgaccggt tacggccagt
      121 gggaatatca gatccagggc aatacctctg aagacaacaa agaaaactcc tggacccgtg
      181 tggcattcgc aggtctgaaa ttccaggatg tgggttcttt cgactacggt cgtaactacg
      241 gcgttgttta cgacgtaact tcttggaccg acgtactgcc agaattcggt ggcgacacct
      301 acggttctga caacttcatg cagcagcgtg gtaacggctt cgcgacctac cgtaacactg
      361 acttcttcgg tctggttgac ggcctgaact ttgctgttca gtaccagggt aaaaacggta
      421 gcgtaagcgg cgaaggcatg accaacaacg gtcgtggtgc tctgcgtcag aacggcgacg
      481 gcgttggcgg atctatcact tatgattacg aaggcttcgg tatcggtggt gcgatctcca
      541 gctccaaacg tactgatgat cagaacagcc cgctgtacat cggtaacggc gaccgtgctg
      601 aaacctacac cggtggtctg aaatacgacg ctaacaacat ctacctggct gctcagtaca
      661 cccagaccta taacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa gcacagaact
      721 ttgaagctgt tgctcagtac cagttcgact tcggtctgcg tccgtctgta gcatacctgc
      781 agtctaaagg taaaaacctg ggtaccatcg ctggtcgtaa ctacgacgac gaagatatcc
      841 tgaaatatgt tgatgttggt gctacctact acttcaacaa aaacatgtcc acctacgttg
      901 actacaaaat caacctgctg gacgacaacc agttcactcg tgacgctggc atcaacactg
      961 ataacatcgt agctctgggt ctggtttacc agttctaatc tcgattgata tcgaacaaag
     1021 ggcctgcggg cccttttttc attgttttca gcgtacaaac tcattttttt ggtgtactct
     1081 tgcgaccgtt cgcatgagga taatcacgta tggaaataag ctttacccgc gtggcactgc
     1141 tggctgccgc gctcttcttt gttggttgcg atcaaaaacc acaacccgcc aaaacccacg
     1201 ctactgaagt taccgttcta gaaggcaaaa ccatgggtac cttctggcgt gccagcatcc
     1261 cgggcattga tgccaaacgc agtgccgaac ttaaagaaaa gattcagacc cagctggacg
     1321 ccgacgatca gctgctttcg acctataaaa aagattccgc gctgatgcgc tttaacgatt
     1381 cgcaaagttt atcgccgtgg ccggtaagtg aagcgatggc cgatatcgtc accacctcgc
     1441 tgcgcattgg cacgaagacc gatggcgcga tggatataac cgtcgggccg ctggtgaatc
     1501 tgtggggctt tggcccggaa caacagtcgg ttcaaattcc gagccaggaa cagatcgatg
     1561 cgatgaaagc caaaaccggc ttacagcacc tgacggtcat taatcagtcg catcagcaat
     1621 atctgcaaaa agacctgccg gatttaaatg tcgatctctc caccgtcggt gaaggttatg
     1681 cggcggatca cctggcacgc ttgatggagc aggaagggat ttcccgctat ctggtgtcgg
     1741 tgggcggcgc gctgaacagc cgtggtatga acggtgaagg cctgccgtgg cgggtagcga
     1801 ttcaaaaacc aaccgataaa gaaaacgcgg ttcaggctgt ggtggatatc aacggtcatg
     1861 ggattagcac ctctggcagc taccgtaact attacgaact ggacggcaaa cgtctttccc
     1921 atgttatcga tccgcaaacc gggcgtccca tcgaacacaa tctggtatcc gtgacggtga
     1981 ttgctccgac ggcgctggaa gccgatgcct gggatactgg cttgatggta ctcgggccgg
     2041 agaaagccaa agaggttgtt cgccgggaag ggctggcggt ttatatgatc accaaagaag
     2101 gcgatagctt taaaacctgg atgtcaccac agtttaaaag cttccttatc agcgaaaaaa
     2161 attaaagcgc aagattgttg gtttttgcgt gatggtgacc gggcagacta aaggctatcc
     2221 ttaaccaggg agctgattat gaaaaacgcc acatgcttaa ctgacgatca acgctggcaa
     2281 tctgtcttag cccgcgaccc gaatgccgac ggcgaattcg ttttcgccgt gcgcaccacg
     2341 ggtatctttt gccgtccgtc ttgccgcgcc agacatgcct tgcgggaaaa tgtctccttc
     2401 tacgcaaatg ccagcgaggc actcgccgcc ggatttcgcc cctgcaaacg ttgtcagcca
     2461 gacaaagcca atccccggca acatcggttg gataaaatca cccacgcgtg tcgactgctg
     2521 gaacaggaaa cgcctgtaac gctggaagcc ttagccgacc aggtggcgat gagtccattt
     2581 catctacatc ggttgtttaa agcgactacc ggaatgacgc ctaaagcctg gcaacaggcc
     2641 tggcgcgctc gccgtttgcg cgaatcgctg gcgaaagggg agagcgtgac gacgtctatt
     2701 cttaacgccg gattccccga cagcagcagt tactaccgca aagccgatga aacgctgggc
     2761 atgacggcta aacaattccg tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct
     2821 gattgtgagc tgggtcgttg cctggtggca gaaagcgagc gggggatttg cgcgatattg
     2881 ctgggcgatg atgacgcgac attaatcagc gagttgcagc agatgtttcc cgctgccgac
     2941 aacgcgcctg ccgatctgac gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat
     3001 caacgcgata cgccgctgac gttaccgctg gacattcgcg gcactgcttt tcagcaacaa
     3061 gtctggcagg cactgcgcac gataccttgt ggtgaaaccg tcagttatca gcaactggct
     3121 aacgccatcg gcaaaccgaa agcggtacgg gccgttgcca gcgcctgtgc cgccaacaag
     3181 ctggctatcg taataccctg tcatcgagtg gtccgtggtg atggcacact ttccggttac
     3241 cgctggggcg tgtcgcgtaa agcgcaactg ctgcgccgcg aagctgaaaa tgaggagagg
     3301 taatgttgga tctgtttgcc gatgctgaac cgtggcaaga gccactggcg gctggtgcgg
     3361 taattttacg gcgttttgct tttaacgctg cggagcaact gatccgcgat attaatgacg
     3421 ttgccagcca gtcgccgttt cgccagatgg tcacccccgg gggatatacc atgtcggtgg
     3481 cgatgaccaa ctgtgggcat ctgggctgga cgacccatcg gcaaggttat ctctattcgc
     3541 ccattgatcc gcaaacaaat aaaccgtggc ccgccatgcc acaaagcttt catgatttat
     3601 gtcaacgtgc ggctacggcg gcgggctatc cagatttcca gccagatgct tgtcttatca
     3661 accgctacgc tcctggcgcg aaactgtcgc tgcatcagga taaagacgaa ccggatctgc
     3721 gcgcgccgat tgtttctgtt tctcttggct tacccgcgat ttttcaattt ggtggcctga
     3781 aacgtaatga tccgctcaaa cgtttgttgt tggaacatgg cgatgtggtg gtatggggcg
     3841 gtgaatcgcg gctgttttat cacggcattc aaccgctgaa agcggggttt catccactca
     3901 ccggcgactg ccgttacaac ctgacattcc gtcaggcagg taaaaaagaa taaaaataag
     3961 aattattatt gctgtgcgcg cgaagatgtt taaactgcgg gctgtaattc attgtccggg
     4021 ttttctgcat ggaacttctt gtacttgtct ggcggcagta tcgctggcca tttatcagtg
     4081 tgatggagct aagcctcgcc agtgcggctt taggcattgg cttaattgct tttatcaatc
     4141 agcgccttat cgaaacggcg gataccagtc tgctggtgtt gccggagttt ctgggattat
     4201 tgctgctgtt gatggcaatc actctcggat cgcaactggc gctcaccact ttggggcatc
     4261 acttcgttta ccgactgcgt agtgaattta tcaagcggat tctcgatacc cacgtcgagc
     4321 gcattgaaca actcggtagc gcctcgttgc tggcgggatt aaccagcgat gtgcgcaata
     4381 tcaccattgc ttttgtgcgt ctgccggaac tggtgcaggg gatcattctc actatcggtt
     4441 cagcggcgta tctgtggatg ctgtcgggca aaatgttgct ggtaacggct atctggatgg
     4501 cggtcaccat ctggggcggt tttgtgctgg tggcgcgggt gtacaaacat atggcgaccc
     4561 tgcgtgaaac cgaagataag ctgtacacgg attttcaaac cgtactcgaa gggcgcaaag
     4621 agctgactct gaaccgtgaa cgcgccgagt atgtgtttaa caacctctac attcctgatg
     4681 cgcaagagta tcgccatcat attatccgcg cagacacctt ccatcttagt gccgtgaact
     4741 ggtcaaacat catgatgctg ggcgctatcg gcctggtgtt ctggatggcg aacagcctcg
     4801 gttgggctga taccaacgtt gctgcgacct attcgttgac gctgttattc ctgcgtacgc
     4861 cgctgctttc ggcggttggc gcattgccga cgctgctgac ggcgcaggtg gcgtttaaca
     4921 agctgaacaa attcgcgctc gcgcctttca aagcagagtt tccgcgcccg caggcgtttc
     4981 ccaactggca aacgctggag ctgcgtaatg tgacgtttag ctatcaggat aacgcgtttt
     5041 ccgttgggcc gattaacctc accattaaac gtggcgagct gctgtttctg attggtggca
     5101 acggtagtgg aaaatcgacg ctggcgatgt tgctgacggg cttgtaccag ccacaaagcg
     5161 gcgaaatctt gctggatggc aaacctgtca gcggcgaaca accggaagat tatcgcaaac
     5221 tgttttcggc agtgtttacc gatgtctggc tgtttgatca actgctgggg ccggaggggc
     5281 aacccgctaa cccgcaactg gttgagaagt ggctggcgca gctgaaaatg gctcataagc
     5341 ttgagttaag caacgggcgt attgttaacc tgaagttatc aaaagggcag aaaaaacgcg
     5401 tggcgctgtt gctggcgctg gcagaagaac gcgatattat cctgctggat gaatgggcgg
     5461 cggatcagga tccacacttc cgtcgtgagt tttatcaggt gttgctgccg ctgatgcagg
     5521 agatgggtaa aactattttc gccatcagtc atgatgatca ttactttatc cacgccgacc
     5581 gcctgctgga aatgcgcaat gggcaactta gcgagctgac gggcgaagag cgcgatgccg
     5641 cttcgcgtga tgccgttgcc cggacggcat aatattacga cgctgacctg gcggactgct
     5701 gccgccaggt caatattcta aaaatccctt catttctaat aaccctataa ttaatcaacg
     5761 aaattataat gtttctaaaa ttagaatata atttataaac attatttaaa tgttgttact
     5821 ttagtgttac cgttgatgcc gcgcaaatct cacctgcaat aaagcgacta aaagtaaggc
     5881 attaacaaga tgaaaaaagt gactgccatg ctcttctcga tggccgtggg gcttaatgcc
     5941 gtttcgatgg cggcaaaagc gaaagcgtcc gaggagcagg aaactgatgt actgttgatt
     6001 ggcggcggca ttatgagcgc cacgttgggg acctatttac gcgagctgga gcctgaatgg
     6061 tcgatgacca tggtggagcg cctggagggt gtcgcgcagg agagttcgaa cggctggaac
     6121 aacgccggaa ccgggcattc tgcactgatg gaactgaact acaccccgca aaacgccgat
     6181 ggcagtatca gtattgaaaa agcagtcgcc attaacgaag catttcagat ttcccgccag
     6241 ttctgggcgc accaggtcga gcgcggcgtg ctgcgtactc cgcgttcatt tatcaatacc
     6301 gttccgcata tgagctttgt ctggggcgag gataacgtta atttcctgcg cgcccgttac
     6361 gccgcgttgc aacaaagctc gctgtttcgc ggtatgcgtt actctgaaga tcacgcgcag
     6421 atcaaagagt gggcaccgtt agtgatggaa gggcgcgatc cgcaacagaa agtggcagcc
     6481 acgcgtacgg aaattggtac cgatgtgaac tacggcgaga tcacccgcca gttaattgct
     6541 tccttgcaga agaaatctaa cttctcgctg caactcagca gcgaagtccg cgccctaaag
     6601 cgtaatgacg ataacacctg gaccgttacc gttgccgatc tgaaaaatgg cactgcacag
     6661 aacattcgtg ccaaatttgt ctttatcggc gcgggcggtg cggcgctgaa gctgttacag
     6721 gaatcgggga ttccggaagc gaaagactac gccggtttcc cggtgggcgg acagttcctt
     6781 gtttcggaaa acccggacgt ggttaatcac catctggcga aggtttacgg taaagcatcc
     6841 gttggcgcac caccgatgtc ggttccgcat atcgataccc gcgttctgga cggtaaacgc
     6901 gtagtgctgt ttgggccatt tgccaccttc tcaaccaaat tcctcaaaaa cggttcattg
     6961 tgggatctga tgagttccac caccacctct aacgtgatgc cgatgatgca cgtcgggctg
     7021 gataatttcg atctggtgaa atacttggtg agtcaggtga tgttgagtga agaggatcgt
     7081 tttgaagcgt tgaaagagta ctatccgcag gcgaaaaaag aggactggcg tttgtggcaa
     7141 gcggggcagc gtgtgcagat tatcaagcgt gatgccgata aaggtggtgt actgcgtctg
     7201 ggtactgaag tggtcagtga ccagcaaggg accattgccg cgctgctggg ggcatcgcca
     7261 ggggcgtcaa ccgctgcgcc gattatgttg gatctgctgg aaaaagtatt tggcgatcgc
     7321 gtttccagcc cgcaatggca ggctacgttg aaagcgatcg ttccatctta tggacgcaag
     7381 ctgaacggtg atgtagcggc aacagaacgc gagttgcagt acaccagcga agtgctgggc
     7441 ctgaaatacg acaagccgca ggcagcagat agtacgccga aaccgcagtt gaaaccgcaa
     7501 cccgttcaaa aagaagtggc ggatattgcg ttgtaatgat gtgccacatc cggcatggta
     7561 tgccggatgt ggcgtaaccg ccttatccga cctacggggt ggagcacttc acagcggtgt
     7621 aggcctgata agacgcgtca gcgtcgcatc aggcaaccgg ctcgggcgtt agatgtcgga
     7681 tgcggcgtaa ccgccttatc caacctacgg ggtggagcac ttcacagcgg tgtaggcctg
     7741 ataagacgcg ccagcgtcgc atcaggcaac cggcttgggc gttagatgtc ggatgcggcg
     7801 tgaccgcctt atccgaccta cggggtgacg cacttcacag cagtttagcg aactaccgcg
     7861 ttgtcaattt tctcttccgc cttccagacg cggtacttca catctacatt gtctggcgta
     7921 tacaccacga tcggcagctt gctgttgtaa cgcagcattc cagcatcgcc cagatacgcg
     7981 gtgacaaatt tcttctcttt cttgccatcc gggcaggcca tcatcgtgga aaccggcgag
     8041 ctaactttat caaagacata ataatcatag ccccagcctt ccagcgtttt gttttccagc
     8101 ttcccgccga gacgatgcaa attgcaatcg acttccagcg tctgaccggt taacagttct
     8161 actttcaggg tagattcatc ttcttgcggg gttaactgaa tcacctgacg cttcatccct
     8221 ttttcagctt gtggataagg cgcgattttt tccagtggct ggacgctttc tgccgcccag
     8281 gcggaagtgg tggcgaaagc ggcaaacaat actgcaggta gaatggtctt catttttttg
     8341 ctttccttct tcatttatag gttttcaccg atgatgtcgc tgaaggttaa catattcatc
     8401 agatttatta atttgcttta tgaataactt aaagaacatt cagacaattt tacctgctct
     8461 tttagtgtta aattctgatg agggagcaaa ccagcagaaa tatctctggt gaattaatcg
     8521 ctatataaat atgtttataa ccatttgaaa tgtgagcaaa agcccgtttt tgccacgctt
     8581 cccgccactc ttttgatcct gctacaggtt ttaccccgat cggggtatgc atctttgaca
     8641 catcctttaa tatcttagcg gctataaaaa tggcttatta attatgcggt ttatttggtc
     8701 gctctcaatt ttcagagcgc gttaatgatg gaaggtcaat gtgaagattg atgcatcccg
     8761 tcggggcata ctcactggtc gctggcgcaa agccagtaac ggtatccgtc cgccctggtc
     8821 cggtgatgaa tctcattttc tgacccattg cacccgttgc gacgcctgta ttaatgcttg
     8881 tgaaaacaac attctgcaac gcggcgcggg cggctatccg agcgttaatt tcaaaaataa
     8941 cgagtgctgc ttctgttacg cctgcgcgca ggcttgcccc gaatcgctat tttctccgcg
     9001 ccacaccagg gcctgggatt tgcagttcac cattggggac gcgtgcctgg cgtatcagtc
     9061 agttgaatgc cgccgctgtc aggatagctg tgagccaatg gcaattatct ttcgcccgac
     9121 gctctccggg atctaccagc cgcaacttaa tagccaactt tgcaacggat gtggagcatg
     9181 tgcagccagc tgtccggtat cagccataac tgcggagtat ctccatgcac actaactggc
     9241 aagtttgcag cctggtcgtg caggccaaaa gcgaacgaat ttcagacatc agcacccaac
     9301 tgaacgcctt tcccggctgt gaagttgctg tcagcgacgc gccgagcggt cagttgattg
     9361 tggtggtgga agcagaagac agcgaaacgc tgatccaaac cattgagtca gtacgcaacg
     9421 tagagggcgt gctggcggtg tcgctggttt atcaccagca ggaagagcaa ggtgaggaaa
     9481 caccatgaaa ctcagtcgtc gtagctttat gaaagctaac gccgttgcgg ccgctgcggc
     9541 ggctgccggt ctcagcgtgc cgggcgttgc ccgcgccgtt gttggtcagc aggaagccat
     9601 taaatgggat aaagcgccgt gccgtttctg cggtactggt tgcggcgttc tggtcggaac
     9661 gcagcagggg cgtgtggtgg cctgtcaggg cgacccggac gcaccggtta accgtggcct
     9721 gaactgcatt aagggctatt tcctgcccaa gatcatgtac ggtaaagacc gtttgacgca
     9781 gccgctgctg cgtatgaaaa acggtaaata tgacaaagaa ggcgaattta ccccaatcac
     9841 ctgggatcag gccttcgatg tgatggaaga gaaattcaaa accgccctga aagaaaaagg
     9901 gccggaatcg atcggtatgt tcggttctgg tcagtggact atctgggaag gttatgccgc
     9961 gtccaagctg tttaaagcgg gcttccgttc gaacaacatc gacccgaacg cgcgtcactg
    10021 tatggcgtcg gcagtagttg gctttatgcg tacctttggt atggatgagc cgatgggctg
    10081 ctatgacgac atcgagcagg ctgacgcgtt tgtgctgtgg ggcgcaaaca tggcggagat
    10141 gcacccgatc ctctggtcgc gcatcactaa ccgtcgtctc tctaatcaga acgtcaccgt
    10201 ggcggtgctt tctacctacc agcatcgtag cttcgagctg gcggataacg gcatcatctt
    10261 tacgccacag tctgacctgg tgatcctgaa ctacatcgcc aactatatca ttcaaaacaa
    10321 tgcgataaat caggacttct tcagcaaaca cgttaacctg cgcaaagggg cgacggacat
    10381 cggctacggt ttacgtccga cccatccgct ggaaaaagca gcgaagaatc caggttctga
    10441 cgcctccgaa ccgatgagct ttgaagatta caaagccttc gttgccgagt atacgctgga
    10501 aaaaaccgct gaaatgaccg gcgtgccgaa agaccagtta gaacaactgg cgcagctgta
    10561 tgccgatccg aacaagaaag tcatctccta ctggacgatg ggcttcaacc agcatactcg
    10621 tggcgtgtgg gctaacaacc tggtctacaa cctgcacctg ctgaccggca aaatttccca
    10681 gccgggttgc ggtccgttct ccctgaccgg gcaaccttct gcgtgtggta ctgcgcgtga
    10741 agtgggcacc tttgctcacc gtctgcctgc ggacatggtg gtgaccaacg agaaacaccg
    10801 tgatatctgc gagaagaagt ggaatatccc gagcggcacc attccggcga aaatcggtct
    10861 gcacgcggtg gcacaagacc gtgcgctgaa agacggcaaa ctgaatgttt actggaccat
    10921 gtgtaccaac aacatgcagg ccgggccgaa catcaatgaa gagcgtatgc cgggctggcg
    10981 cgatccgcgc aacttcatca tcgtctccga tccgtatccg acagtcagtg cgctggccgc
    11041 cgacttgatc ctgccgaccg caatgtgggt agagaaagag ggtgcttacg gtaacgccga
    11101 acgccgtact cagttctggc gtcagcaggt acaggcaccg ggcgaagcga aatcggatct
    11161 ctggcagtta gtccagttct cccgccgctt caaaactgaa gaagtgtggc cggaagagct
    11221 gctagcgaag aaaccggaac tgcgtggcaa aacgctgtac gaagtgcttt atgccacacc
    11281 ggaagtgagc aaattcccgg tatccgaact ggcggaagat cagctgaacg atgaatcccg
    11341 cgagctgggc ttctatctgc aaaaagggct gttcgaagag tacgcatggt ttggtcgcgg
    11401 tcacggtcac gatctcgcgc cgttcgatga ctaccacaaa gcgcgcggtc tgcgctggcc
    11461 ggtggtgaac ggtaaagaaa cgcagtggcg ttacagcgaa ggtaacgacc cgtacgtgaa
    11521 agcgggcgaa ggctataagt tctacggtaa accggatggt aaagcggtga tcttcgcgct
    11581 gccgttcgaa ccggcggcgg aagcaccgga tgaagagtac gacctgtggc tctctaccgg
    11641 acgcgttctg gagcactggc acactggcag tatgactcgc cgtgtgccgg aactgcaccg
    11701 cgccttcccg gaagcggtct tgtttattca cccgctggat gcgaaagcgc gcgatctgcg
    11761 ccgtggcgac aaagtgaaag tggtttctcg ccgtggcgaa gtgatctcga ttgttgaaac
    11821 gcgcggtcgt aaccgtccgc cacagggcct ggtgtacatg ccgttcttcg acgccgcaca
    11881 gctggttaac aaactaacgc tggacgcgac cgatccgctc tcgaaagaga cggacttcaa
    11941 gaagtgcgca gtcaaactgg agaaggtgta agccattatg tcccggtcag cgaaacctca
    12001 aaatggtcgc cgccgctttc tgcgcgatgt tgttcgcaca gcaggcgggc tggctgccgt
    12061 gggtgtggcg ctggggttac aacagcaaac cgcacgcgca tctggcgtgc ggttgcgccc
    12121 gcccggagcc ataaacgaga acgcctttgc cagtgcctgt gtgcgttgcg gtcagtgtgt
    12181 tcaggcttgc ccttacgaca ccttaaaact ggcgacgctg gcttctggcc tgtcggcggg
    12241 cacgccatat tttgtcgcac gggatattcc ttgcgaaatg tgtgaggaca ttccgtgcgc
    12301 caaagtgtgc ccgagtggtg cgctggatcg tgagatcgaa tcgatcgacg acgcgcggat
    12361 ggggctggcg gtgctggtgg accaggaaaa ctgtctcaac tttcaggggc tgcgctgcga
    12421 tgtttgttat cgcgaatgcc cgaaaattga tgaggccatc accctggagc tggagcgcaa
    12481 cacgcgtacc ggtaagcacg cccgctttct gccgacggtt cacagcgacg cctgtaccgg
    12541 ctgcggtaag tgcgaaaaag tgtgcgtgct ggaacaaccg gcaatcaagg tgttaccgct
    12601 gtcactggcg aaaggggagt taggtcacca ttaccgcttc ggctggctgg aggggaacaa
    12661 tggcaaatcg taaacgtgac gccgggcgcg aggcgctggt gaaaaaaggc tggtggcgca
    12721 gtcaccgttg gctggtgtta cgtcgccttt gtcagttctt cgtgctgggg atgtttttga
    12781 gcggtccgtg gtttggtgtg tggatcttac acggcaacta cagctgtagc ctgttattcg
    12841 acaccgttcc gctgaccgat ccgctgatga cgctgcaaag tcttgccagt ggtcatctgc
    12901 ctgccacggt ggcgttgacc ggtgcggtga taatcactgt gctctacgcc ctggcaggaa
    12961 agcgattatt ttgcagctgg gtctgcccgc tgaacccgat taccgactta gcgaactggt
    13021 tacgcaggcg ctttgacctc aatcagtctg cgacgatccc tcgccacatt cgctacgtgc
    13081 tgttggtggt cattctgctg ggatcagcct taaccggcac gctcatctgg gaatggatca
    13141 accccgtttc cctgatgggg cgtagcctgg taatgggctt cggcagcggc gcgctgctta
    13201 ttctcgcgct gtttttattt gatctactgg tcgttgaaca cggctggtgc gggcacattt
    13261 gtccggttgg cgcgctgtat ggcgtgctgg gtagcaaagg tgtgattacc gttgcggcca
    13321 gcgatcggca gaaatgtaac cgctgtatgg attgttttca tgtttgcccg gaaccgcatg
    13381 tgctacgtgc cccggtgctg gatgaacaaa gcccggtgca ggtcaccagc cgcgattgca
    13441 tgacgtgcgg tcgctgcgtg gatgtctgtt ctgaggatgt atttacaata actacacgat
    13501 ggagttcggg agcgaaatca tgaaaagcca tgacctgaag aaagcgctgt gtcaatggac
    13561 ggcgatgctg gccctggtgg taagcggcgc ggtttgggcg gctaatggcg tcgattttag
    13621 ccagtcgccg gaagtatccg ggacgcagga aggggccatt cggatgccaa aagagcagga
    13681 tcggatgccg ctgaactatg tgaatcagcc gccaatgatc ccgcatagcg ttgaaggtta
    13741 tcaggtaacg accaatacca accgctgctt gcagtgccac ggtgtcgaaa gctatcgcac
    13801 cactggcgcg ccgcgtatca gtcctactca ctttatggac agcgacggca aagtgggcgc
    13861 ggaagtggct ccgcgtcgtt atttctgtct gcaatgtcac gtaccgcagg ccgataccgc
    13921 gccaatcgtg gggaatacct ttaccccatc aaaaggttac gggaaataag aggttattat
    13981 gggaaattct gaccgtaagc ctggtctgat taagcgcctg tggaaatggt ggcgtacccc
    14041 cagccgtctg gcgctgggaa cgctgctgtt gatcggtttt gtcggcggca tcgtcttctg
    14101 gggcggcttt aacaccggaa tggaaaaagc caataccgaa gagttctgca ttagctgcca
    14161 cgaaatgcgt aacacggtgt atcaggaata catggattcc gtgcactaca acaaccgtag
    14221 cggcgtccgt gcgacctgtc cggattgtca cgttccgcac gagtttgtgc cgaagatgat
    14281 acgcaagctc aaagcaagta aagagctgta tggtaaaatt tttggcgtta ttgacacgcc
    14341 gcagaaattt gaagctcatc gtctgacaat ggcacagaat gagtggcggc gcatgaagga
    14401 caataactcg caggagtgcc gtaactgtca caacttcgag tatatggata caaccgccca
    14461 gaaatcggtt gccgcgaaga tgcatgacca ggcggtgaaa gatgggcaaa cctgtattga
    14521 ttgccataaa gggatagcgc acaagctgcc cgatatgcgt gaagtcgagc caggttttta
    14581 acagggttat tgcgtgggta tgcttgaagc cagagagtta ctttgtgagc gggatgaacg
    14641 aaccttattt agtggcttgt catttacgct gaacgcagga gagtgggtac aaatcaccgg
    14701 tagcaacggc gcggggaaga caacgcttct ccgtttgctg acggggttgt ctcgccctga
    14761 cgcaggcgag gttctctggc aagggcagcc cttgcatcag gtacgcgaca gctaccatca
    14821 aaacctgtta tggataggcc atcagccggg gatcaaaacc cggctgacgg cgttagaaaa
    14881 tctgcacttt tatcatcgcg atggcgatac cgcacaatgt ctggaagccc tggcgcaggc
    14941 cgggcttgcc ggattcgacg atattccggt aaatcaactt tctgccgggc aacaacgccg
    15001 cgtcgcttta gcgcgtctgt ggctgacccg tgccacgtta tggatcctcg acgagccttt
    15061 taccgcgatt gacgttaacg gcgtcgatcg tctgacccag cgtatggcgc agcatacgga
    15121 gcaggggggg attgtgattc tgactaccca ccagccgctc aacgttgctg aaagtaaaat
    15181 tcgccgcatt tcactgacgc aaacgagggc cgcatgatgt tctggcgcat tttccgtctt
    15241 gagctgcgtg tagcgtttcg ccatagcgcc gaaatcgcca acccgctgtg gttcttcctg
    15301 attgtaatta ccctttttcc gctcagtatc ggtccggagc cgcaactgct ggcgcgtatt
    15361 gcaccgggca ttatctgggt tgctgcgctg ctttcatcct tgctggcgct ggaacgactg
    15421 ttccgtgacg atttgcagga cggcagtctt gaacaattga tgttgttgcc gttacccttg
    15481 cccgccgttg tgctggcgaa ggtgatggcg cactggatgg taaccggtct gccgttactc
    15541 atcctttcgc cactggtagc aatgctactg ggaatggatg tttatggctg gcaagtgatg
    15601 gcgctgacgc tgctgctggg aacgcctacg cttggctttc tcggtgcacc gggtgtggcg
    15661 ttgacagtgg gacttaagcg cggtggtgtg ctgctcagca tactggtgtt accgctgact
    15721 atcccattac tcatctttgc caccgccgcg atggacgcgg cttccatgca tttgcccgtt
    15781 gacgggtatc tggcaatttt aggcgcgttg ctggcaggca ccgcgacatt aagtcctttt
    15841 gcgacggcgg cagcgttacg aatcagcatt caataacgct ggattactga tttttgttcg
    15901 agtctggtat cgaaactatg tggaaaacac tgcatcaact ggcgatccca ccacggctgt
    15961 atcaaatctg tggctggttt ataccgtggc tggcaattgc cagtgtggtc gtgcttaccg
    16021 tcggctggat ctggggattc ggctttgctc cggctgatta tcagcaggga aatagctacc
    16081 gcattatcta cctgcatgtg cctgcggcga tctggtcgat gggcatttat gcatcaatgg
    16141 cagtggcagc gtttattggc cttgtctggc agatgaaaat ggccaacctg gcggtggcgg
    16201 cgatggcccc cattggtgcc gtgtttacct ttattgccct ggttaccggc tctgcatggg
    16261 gaaaaccgat gtggggcacc tggtgggtat gggatgcacg tctgacttct gaactggtgc
    16321 tgctgttttt gtatgtgggt gtgattgccc tgtggcacgc cttcgacgac cgccgtctgg
    16381 cgggccgtgc ggcaggtatc ctggtgctga ttggcgtggt gaatctgccg attattcatt
    16441 actccgtgga gtggtggaac accctgcatc agggatcaac gcggatgcag caaagtatcg
    16501 atccggcgat gcgttcgccg ctgcgctggt cgatttttgg cttcctgctc ctgtctgcca
    16561 cgctgacgct gatgcggatg cgtaatttga ttttgctgat ggaaaaacgc cgtccgtggg
    16621 tgagtgaact gatactgaaa agaggccgta aatgacccct gcatttgctt cctggaatga
    16681 atttttcgca atgggcggtt acgccttttt tgtctggctg gcggtggtga tgaccgttat
    16741 tccgctggtg gttttggtcg tgcactcggt gatgcaacat cgcgcaattc tgcgtggtgt
    16801 ggcgcaacag cgggcgcgtg aggcgcgttt acgtgctgcg caacagcagg aggctgcatg
    16861 aatattcgcc gtaaaaaccg cttgtggatt gcctgtgccg tgttggcagg gctggcgctg
    16921 actatcggtc tggtgctata tgcgctgcgc tcgaatatcg atctctttta tacgccgggg
    16981 gaaattctct acggcaagcg tgaaactcag caaatgccgg aagtcggtca gcgtttgcgc
    17041 gttggcggga tggtgatgct gggtagtgtg cagcgcgatc ccaattcgct gaaagtgacc
    17101 ttcactattt acgatgctga aggctcagtg gatgtctctt acgaaggcat tttgccggat
    17161 ctgttccgtg aagggcaggg cgttgtggtg cagggcgagc tggaaaaagg caatcatatc
    17221 ctcgcgaaag aagtgctggc gaaacacgac gaaaactaca cgccgccaga agttgagaaa
    17281 gcgatggaag ctaaccaccg tcgcccggcg agtgtttata aggacccagc atcatgatgc
    17341 cggaaattgg aaacgggctg ctgtgtctgg cgttagggat tgcgctgctg ctgtccgtgt
    17401 atccgctatg gggcgtggcg cgcggagatg cgcgcatgat cgcgtcttcc cgcttgtttg
    17461 cctggctgct gtttatgtct gtggctggcg catttctggt gctggtcaat gctttcgtgg
    17521 tcaacgactt taccgtcacc tatgttgcca gctactccaa tacccagctt ccggtgtggt
    17581 atcgcgtggc ggctacctgg ggcgcgcatg aaggctcgct actgctgtgg gtgctgctga
    17641 tgagcggctg gacctttgcg gtggcgattt ttagtcagcg tattccgctg gatattgtgg
    17701 cccgcgtact ggcgataatg gggatggtca gcgtcggctt tttgctgttc attctcttta
    17761 cctctaaccc attctcacgc acgctgccga acttcccgat tgaagggcgc gatcttaacc
    17821 cgctgttaca ggacccgggg ctgatcttcc atccgcctct gctttatatg gggtacgtgg
    17881 gtttctcggt ggcgtttgct tttgccattg cttctttgct gagcgggcgt ctggacagca
    17941 cttatgcgcg ttttactcgc ccgtggacgc tggcggcgtg gattttcctg acgctcggca
    18001 ttgtgctcgg ttccgcatgg gcctattacg aactcggctg ggggggctgg tggttctggg
    18061 atccggtaga aaacgcctcg tttatgccgt ggctggtggg gactgcgctg atgcactcac
    18121 tggcggtcac tgaacaacgc gccagcttca aagcgtggac attactgctg gcaatcagtg
    18181 ccttctcgtt gtgtctgttg gggactttcc tcgtgcgttc cggcgtgctg gtatcggtac
    18241 acgcgtttgc gtctgatccg gcgcgcggta tgtttatcct cgcctttatg gtgctggtga
    18301 ttggaggttc gctgctgctg tttgccgcgc gtggacacaa agttcgctca cgcgtaaaca
    18361 atgcgctgtg gtcgcgggaa tctctgctgt tagcgaacaa cgttttgctg gtcgccgcga
    18421 tgctggtggt gttgctgggg acgctgctgc cgctggtgca caagcaactg ggactgggca
    18481 gtatttcgat tggcgaaccg ttcttcaaca ccatgtttac ctggctgatg gtgccgtttg
    18541 cgctactgct gggtgtcggt cctctggtgc gctgggggcg cgatcgccca cgtaaaatcc
    18601 gcaatttatt gattatcgcc ttcatctcaa cgctggtgct gtctctgctg ttgccgtggc
    18661 tgtttgagag caaagttgtg gcgatgacgg tgctcggcct ggcaatggcc tgctggattg
    18721 cggtgctggc aattgcggaa gctgcgctgc gtatttcacg cggcacgaaa accaccttca
    18781 gttattgggg gatggtggct gctcacctgg ggctggcagt gacaattgtt ggcattgctt
    18841 ttagccagaa ctatagcgtt gagcgtgatg tgcgcatgaa gtccggcgat agcatcgata
    18901 ttcatgaata tcgcttcacc ttccgtgatg tcaaagaggt gactggcccg aactggcgtg
    18961 gcggtgtggc gactatcggc gtaacgcgag agggtaaacc ggaaacagtg ctgtatgcgg
    19021 aaaaacgtta ttacaacact gccgggtcga tgatgaccga agcggcgatt gacggtggca
    19081 tcacgcgtga cctgtacgcc gcgctcggtg aagagctgga aaacggcgcg tgggctgtgc
    19141 gtctttacta caaaccattt gttcgctgga tttgggcggg cgggctgatg atggcgttgg
    19201 gcggactgct gtgtctgttt gatcctcgct atcgtaagcg cgtgagtccg caaaaaactg
    19261 cgccggaggc tgtatgaagc gcaaagtatt gttaattccg ttgattatct tcctggcgat
    19321 tgccgcggcg ctgctgtggc agctggcgcg taatgccgaa ggggatgatc cgaccaatct
    19381 ggaatcggcg ctcattggca agccagtgcc gaagtttcgc ctcgaatcac tggacaatcc
    19441 ggggcagttt tatcaggcgg atgtgctgac tcagggcaaa ccagtgctgc ttaacgtctg
    19501 ggcgacctgg tgtccgacct gccgtgcgga acatcaatat ctgaatcagc tttctgcgca
    19561 gggcatccgc gtagtcggca tgaactataa agacgatcgc cagaaggcaa tcagctggct
    19621 gaaagagctg ggcaatcctt acgcgctaag cctgtttgat ggcgatggca tgttagggct
    19681 ggatctcggt gtctatggcg cgccagaaac gttccttatt gacggcaacg gcatcattcg
    19741 ctatcgccat gcgggcgatc tcaatcctcg cgtctgggaa gaagagatca agccgctgtg
    19801 ggagaaatac agtaaggagg ccgcacaatg aggtttttat tgggcatgct gatgctgatg
    19861 atctccggct cagcgctggc gaccatagac gtgttgcagt ttaaagatga agcacaggaa
    19921 caacagttcc gtcagctcac tgaagaactg cgctgcccga aatgccagaa caacagcatt
    19981 gccgattcca actcgatgat tgccaccgac ctgcgccaga aagtgtatga actgatgcag
    20041 gaaggtaaaa gtaaaaaaga gattgtcgat tatatggtgg cgcgttacgg caacttcgtc
    20101 acttacgatc cgccgttaac gccgctgacc gtgctgctgt gggtgctgcc agtagtagct
    20161 attggcattg gcggttgggt catatacgcc cgctcgcggc gtcgggtacg cgtggtgccg
    20221 gaagcgtttc ctgaacaaag cgtgccggaa ggtaagcgtg ccggatatgt cgcttatttg
    20281 ccgggcattg tggtggcgtt aattgtggct ggcgtcagct actaccagac cggaaattat
    20341 caacaggtga aaatctggca gcaggccacg gcacaggctc cggcgttgct ggacagggcg
    20401 ctggatccga aagctgatcc gctcaacgaa gaagagatgt cgcgccttgc gctggggatg
    20461 cgtactcaac tgcaaaaaaa tccgggagat atagacggct ggattatgtt gggccgcgtt
    20521 ggcatggcgc tgggtaacgc cagtatcgcc accgatgcat acgctactgc gtatcgcctc
    20581 gatccgaaaa acagtgatgc cgcactgggt tatgctgaag cgttgacacg ttcatctgat
    20641 cccaacgaca accgcctcgg cggcgaactg ctacgtcagc tggtgagaac ggaccatagc
    20701 aatatccgtg tgctaagcat gtatgcgttt aatgcctttg agcagcagcg atttggcgaa
    20761 gccgttgccg cgtgggagat gatgttgaaa ctcttacctg ccaacgatac tcgccgtgcg
    20821 gtgattgaac gtagtatcgc gcaggcgatg caacatttgt cgccgcagga gagtaaataa
    20881 aaagaggccc gatggtgatg ccatcgggct atttttattg tgccccgcgt tgttgcagga
    20941 acagaatggt ggccgccacg cgtgagcgga cattgagttt acgcagcaga tttcgaatat
    21001 gaacttttac tgtctgctcg gaaatattca acacagaggc aatctgttta tttgacagcc
    21061 cctgtgccag ctcgtgcaga acatccagct cgcgctccgt cagcacgctg aagggatctt
    21121 cttccgcgcc aaacatttca cgttcacgta agtactgatt gacgcgttcg ctaaagactt
    21181 tgctgccttt cgctccggca cgaatcgctt ccagcaacac ttccgggtcg ctgtctttca
    21241 gcagataacc gtctgcgcct gcgtctatca gcgcaaagac atcgctggag gcatcggata
    21301 cggtcaggat aataatttgc gcggtaacgc catccctgcg caaggcattg agggtatcca
    21361 ggccactcat acctttcata ttgagatcca gcaagatcac gtcgatatcc agtctattcg
    21421 ccagatcgat cgcgctcgcg ccgtcgcccg cttcggcgac cacttcaaag ccaggatcaa
    21481 gctccagtaa ctgacgaaca ccgcgtcgca taagtggatg atcatccaca atcatcacct
    21541 gaaaaggcgt tgcttcaggc atagtagtct cctgaggttt tattagaatc attatgtttg
    21601 agcgtagcgt tggtaacgct accaggcgcg tgaatgttac cccattttat gtatggacat
    21661 gtagtgaaaa ctacaacctt gccgttcgct ttggcatcat tttcattata aagacggaat
    21721 tttgcatcag cttctcatca agaaaaacaa ctaaaaaatc aatcacaata cttgtgcatt
    21781 tctttaaaca gtttttaaaa actgactccg ggtatggagc tatgggtatt ttctgtaccc
    21841 aatgctttta acagcaatta atttcatagg atgaaagctc aatgcatcaa tctggttctg
    21901 tttctctttg tcgttccgca atatctgttc tggtggctac agcgttatat tcacccatag
    21961 cattggcatc aacagttgag tatggtgaga cagttgatgg tgttggcctg gaaaaagata
    22021 tccagctggt ttatgggacc gccaataata cgaaaatcaa tcctggcgga gaacagcata
    22081 taaaagaatt tggtgtaagt agtaatactg aaattaacgg cgggtatcag tacattgaaa
    22141 tgaatggcac tgcagaatac tcagtattaa atgatggtta tcaaattgtt caaatgggtg
    22201 gcgcggtaaa ccagactacg ctcaataatg gagtgctaca ggtttatgg
//
LOCUS       DAJTVJ010000066        22235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000066 DAJTVJ010000000
VERSION     DAJTVJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.066"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..1463
                     /locus_tag="OO448_002445"
     CDS             75..1463
                     /locus_tag="OO448_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6337633.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="OO448_002446"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="OO448_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6337634.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /locus_tag="OO448_002447"
     CDS             complement(2010..2114)
                     /locus_tag="OO448_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337635.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="OO448_002448"
     CDS             2284..2730
                     /locus_tag="OO448_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6337636.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OO448_002449"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OO448_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6337637.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="OO448_002450"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="OO448_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR6337638.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="OO448_002451"
     CDS             4841..5188
                     /locus_tag="OO448_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6337639.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OO448_002452"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OO448_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6337640.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="OO448_002453"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="OO448_002453"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6337641.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="OO448_002454"
     CDS             6705..6803
                     /locus_tag="OO448_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6337642.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="OO448_002455"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="OO448_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6337643.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="OO448_002456"
     CDS             complement(6939..7187)
                     /locus_tag="OO448_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6337644.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="OO448_002457"
     CDS             complement(7335..7517)
                     /locus_tag="OO448_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR6337645.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OO448_002458"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OO448_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCR6337646.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OO448_002459"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OO448_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCR6337647.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OO448_002460"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OO448_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR6337648.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="OO448_002461"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="OO448_002461"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR6337649.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="OO448_002462"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="OO448_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR6337650.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="OO448_002463"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="OO448_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR6337651.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="OO448_002464"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="OO448_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6337652.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OO448_002465"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OO448_002465"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6337653.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OO448_002466"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OO448_002466"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6337654.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OO448_002467"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OO448_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6337655.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OO448_002468"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OO448_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6337656.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21392)
                     /locus_tag="OO448_002469"
     CDS             complement(19482..21392)
                     /locus_tag="OO448_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6337657.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21524..21868
                     /locus_tag="OO448_002470"
     CDS             21524..21868
                     /locus_tag="OO448_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6337658.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5443 a         5698 c         5403 g         5691 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc ataaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacact atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cccccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aggaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattggt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattcg
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
    19561 agaaacgtcg cgccgtaagg acgcatcacc agccgattgt cacaaattac cagcacgccg
    19621 cgatcgtcgg cgtcgcgaat cagtcgccct actccctgct tgagagtaat gaccgcatcc
    19681 ggcagctgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
    19741 tttaacagta gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
    19801 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcgtta
    19861 ccggcgctga caaattgctg caacagttgc cctttgctgg tttccccctg caacaaaacg
    19921 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc atgcgaggta
    19981 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgccagt
    20041 tgccgtgcac aacccggttg attggtttgc ggcaggttgc gcggcacaca gagtaacgcc
    20101 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
    20161 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
    20221 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
    20281 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
    20341 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
    20401 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccag ggtgtcatcg
    20461 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
    20521 taacctggct caccgagttg cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg
    20581 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
    20641 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc
    20701 ggtagctggt gggcttcgtc gaagatcatg acgtccgctt cagggatcaa ctcgccaaat
    20761 ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg
    20821 gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat cgggcagtcg
    20881 ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
    20941 acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg
    21001 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcctg ctgttcgaga
    21061 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc
    21121 aatgcctttg agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
    21181 ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt
    21241 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    21301 acggctaccg ccatctgtcg ctgtggttct cgcggcttaa agcctggtat cgctttcgcc
    21361 agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    21421 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcaacctc acattgtggc
    21481 agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat
    21541 cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct actacactgg
    21601 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcacagat
    21661 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    21721 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    21781 gggtcatgcg ccagtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt
    21841 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    21901 tttatcgata atactggtcg gttttacata aatcgaccag agaataagat attactgttg
    21961 atgcttctgc taattcatta tttatattta taatttcaat tttatctata aaactaacta
    22021 tataaataaa cataaccatg ccgttgtgtt cgccctttga ttagcaactc tggttttctt
    22081 tcttagctga acaatctggt tatagcgtgc ggactgaccg ttaattttct ccttcttact
    22141 ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
    22201 ccatatcgtt ataatccctt tgatattaga tgcaa
//
LOCUS       DAJTVJ010000067        21977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000067 DAJTVJ010000000
VERSION     DAJTVJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.067"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..367)
                     /locus_tag="OO448_002471"
     CDS             complement(<1..367)
                     /locus_tag="OO448_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6337659.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAV"
     gene            663..1439
                     /gene="exuR"
                     /locus_tag="OO448_002472"
     CDS             663..1439
                     /gene="exuR"
                     /locus_tag="OO448_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6337660.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1784..2446
                     /locus_tag="OO448_002473"
     CDS             1784..2446
                     /locus_tag="OO448_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6337661.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2450..2833
                     /gene="mzrA"
                     /locus_tag="OO448_002474"
     CDS             2450..2833
                     /gene="mzrA"
                     /locus_tag="OO448_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6337662.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2980..3348
                     /locus_tag="OO448_002475"
     CDS             2980..3348
                     /locus_tag="OO448_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR6337663.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3386..3691
                     /locus_tag="OO448_002476"
     CDS             3386..3691
                     /locus_tag="OO448_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6337664.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3694..4098
                     /locus_tag="OO448_002477"
     CDS             3694..4098
                     /locus_tag="OO448_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6337665.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4088..4387
                     /locus_tag="OO448_002478"
     CDS             4088..4387
                     /locus_tag="OO448_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6337666.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4573..4965
                     /locus_tag="OO448_002479"
     CDS             4573..4965
                     /locus_tag="OO448_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6337667.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5035..6048
                     /gene="yqjG"
                     /locus_tag="OO448_002480"
     CDS             5035..6048
                     /gene="yqjG"
                     /locus_tag="OO448_002480"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6337668.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6313..6678
                     /locus_tag="OO448_002481"
     CDS             6313..6678
                     /locus_tag="OO448_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6337669.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6920..7276
                     /locus_tag="OO448_002482"
     CDS             6920..7276
                     /locus_tag="OO448_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6337670.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7327..8223)
                     /gene="yhaJ"
                     /locus_tag="OO448_002483"
                     /pseudo
     CDS             complement(7327..8223)
                     /gene="yhaJ"
                     /locus_tag="OO448_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8328..9029
                     /locus_tag="OO448_002484"
     CDS             8328..9029
                     /locus_tag="OO448_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6337671.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9052..9216
                     /locus_tag="OO448_002485"
     CDS             9052..9216
                     /locus_tag="OO448_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337672.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9350..10660)
                     /locus_tag="OO448_002486"
     CDS             complement(9350..10660)
                     /locus_tag="OO448_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6337673.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10688..12019)
                     /locus_tag="OO448_002487"
     CDS             complement(10688..12019)
                     /locus_tag="OO448_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6337674.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12294..13658)
                     /gene="tdcG"
                     /locus_tag="OO448_002488"
     CDS             complement(12294..13658)
                     /gene="tdcG"
                     /locus_tag="OO448_002488"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6337675.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13730..14119)
                     /locus_tag="OO448_002489"
     CDS             complement(13730..14119)
                     /locus_tag="OO448_002489"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6337676.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14133..16427)
                     /gene="tdcE"
                     /locus_tag="OO448_002490"
     CDS             complement(14133..16427)
                     /gene="tdcE"
                     /locus_tag="OO448_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6337677.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16461..17669)
                     /gene="tdcD"
                     /locus_tag="OO448_002491"
     CDS             complement(16461..17669)
                     /gene="tdcD"
                     /locus_tag="OO448_002491"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6337678.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17695..19026)
                     /gene="tdcC"
                     /locus_tag="OO448_002492"
     CDS             complement(17695..19026)
                     /gene="tdcC"
                     /locus_tag="OO448_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6337679.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19048..20037)
                     /gene="tdcB"
                     /locus_tag="OO448_002493"
     CDS             complement(19048..20037)
                     /gene="tdcB"
                     /locus_tag="OO448_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6337680.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20136..21074)
                     /gene="tdcA"
                     /locus_tag="OO448_002494"
     CDS             complement(20136..21074)
                     /gene="tdcA"
                     /locus_tag="OO448_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6337681.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21263..21607
                     /gene="tdcR"
                     /locus_tag="OO448_002495"
     CDS             21263..21607
                     /gene="tdcR"
                     /locus_tag="OO448_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6337682.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5756 a         5618 c         5141 g         5462 t
ORIGIN      
        1 gaaccgcttc aaatttaacg cgataatttt tttctttttg ctctggttcc attgcaacaa
       61 agcgcacagt tttgctacta ccggctggta aagcgaattt aggtggcata acaaccagat
      121 gatttgcatc accggattta atctcaactt cattttcctg aggcgtcgat ggattatcga
      181 tacggaatac tgtcgccttg atgtactgaa cttcattact tttagatatt acgcgaacgt
      241 tgccttcacc ctgactatta atggaaacag ccataggata aacagtcata tttgactgtg
      301 ccaaaaaagg agcgaacacg aataaaagcg gcgtaagccg tgataagaga ttaattttcg
      361 ccaccatata aatttgatct tacaatattg tttccaaaaa gttggtgtat tagagatgta
      421 gatttattgt aagtcaatat aaaaaagcat ttttagttaa tattaaattt aaaggctggg
      481 aattaactta ctgaaattta gtagctttca caatgacgat acttattttt taggaattta
      541 taaatattca caatattaat tcaatacatt aattcaatca ccaaatttat atatgaccaa
      601 tggcataccc atgaataaag tggtatgata acttcgtccg cattccactc cgggagacgc
      661 atatggaaat cactgaacca cgccgtttgt atcaacaact tgccgctgac ctgaaagagc
      721 gcattgaaca gggcgtctat ctggtgggtg ataaactgcc tgccgaacgc tttattgccg
      781 atgaaaagaa cgtcagccga acggtagttc gtgaagccat catcatgctg gaagttgaag
      841 gctatgtgga ggtgcgtaaa ggttcaggta ttcatgtggt ttccaaccag ccgcgccatc
      901 agcaggcggc tgacaataat atggaattcg ccaattacgg tccgtttgag ttgcttcagg
      961 ctcgccagct catcgaaagt aatattgccg agttcgcggc aactcaggta acgaaacaag
     1021 acatcatgaa actgatggcc attcaggaac aggcgcgcgg cgaacaatgc ttccgtgatt
     1081 ccgagtggga tttacagttc cacattcagg tagccctggc gacgcagaac tccgccctgg
     1141 cggccatcgt tgaaaaaatg tggacccagc gtagccataa cccgtactgg aaaaaactac
     1201 acgaacacat tgattcccgt accgttgata actggtgtga tgaccacgat caaattctca
     1261 aggcgttaat tcgcaaagat ccccatgctg ctaagctggc aatgtggcag catctggaaa
     1321 acaccaaaat catgttattt aacgaaacca gcgacgattt cgagttcaat gccgaccgct
     1381 atctattcgc cgaaaacccg gtagtgcatc tcgatacggc caccagtggc agtaaatgaa
     1441 actcctgtcc gacaggcgct gcgacgcgcc tgttcgcttc gtcggtaagc gaaaagtata
     1501 aagtgtcagc ctgtgtaaat cctctcgcca ccctcccctg cattcagcaa aatcagactc
     1561 cacggacatg taattttgat aacgaacaac gttgaccttt gttacaatta gattcaattt
     1621 gaatttatgt ttttgaatgc tttcttatct cacgatttaa cagggaatag ttcaggctgt
     1681 gttgatgtat caaacccgca gaacatacca aaacagcaat aacattgcgg tagtgcatct
     1741 tttaaaacca gcgtggcgtt aaccgattca ccaggaataa tgaatggaac ttttgaccca
     1801 attgctgcaa gccctgtggg cgcaggattt tgaaaccctg gccaatccat cgatgattgg
     1861 catgttgtat tttgtcttgt ttgtaatttt gttccttgaa aacggcttgc ttccagcggc
     1921 ctttttaccg ggcgacagtt tactggtatt ggtcggcgtg ttgattgcga aaggcgcgat
     1981 gggttacccg caaacgattc tgctgctgac cgttgccgcc agcctcggct gctgggtcag
     2041 ctatattcag gggcgatggc tgggcaatac ccgcaccgta caaaactggc tatctcattt
     2101 acccgcgcat tatcatcaac gcgcacacca tctttttcat aaacacggtt tatcggcgct
     2161 gttaattggt cgctttattg cgtttgtcag aacactgctg ccgacgattg ccgggttatc
     2221 agggctgaat aacgcgcgct ttcagttttt caactggatg agcggtctgc tgtgggtatt
     2281 gatcctgaca actctgggtt acatgctcgg caaaacgccg atatttttaa agtacgagga
     2341 ccagctgatg tcatgcctga tgctgctccc ggtggtgctg ctggtgtttg gcctggcagg
     2401 ttctctggtc gtgttatgga aaaagaaata tggaaatcgg gggtaaggga tgcaaatacc
     2461 tcgcatgtcg cttcgccagc tagcctggtc cggcactgtt ttacttctgg tcggcacgct
     2521 gttgctggcc tggtccgcgg ttcgccagca agagtctacg ctggcgattc gtgccgttca
     2581 tcaaggcaca acgatgccag acggtttttc gatctggcat caccttgacg ctcatggcat
     2641 tcctttcaaa agtatcaccc ccaaaaacga cactctgtta attacatttg actccagcga
     2701 ccagagcgcc gccgcaaaag cggtcctcga cagaacattg ccccatggct acatcattgc
     2761 gcagcaggac aataacagtc aggctatgca gtggctgacc cggttacggg ataactctca
     2821 tcgcttcgga taacttcctg ttatccgaaa catatcactc actttggtga tttcaccgta
     2881 actgtctatg attaatgagg cggtggttaa cactaccgct tcgttgtctg gacaatcgtt
     2941 cctttgtaat aggtccgaat cacaatggaa ggttcaagaa tgaaataccg catcgcttta
     3001 gctgtttctc tctttgctct tagtgccggt agttatgcca ctaccctgtg tcaggaaaag
     3061 gagcaaaata tccttaagga gatcagctat gccgaaaaac accaaaacca gaatcgtatt
     3121 gacggtctga ataaagccct gagtgaagtc cgggccaact gttcagatag ccagctgcgt
     3181 gccgatcatc acaagaaaat cgcaaagcag aaagatgagg tggcggaacg ccagcaagat
     3241 ttagccgagg cgaagcaaaa aggcgatgcc gataagattg ccaaacgcga acggaaactg
     3301 gcagaagcgc aggaagagct gaaaaagctg gaagcgcgcg actactaact cacaatagtc
     3361 actacttact cacctggaga aaactatgtc gaaagaacac actacggaac atctgcgtgc
     3421 tgagttgaaa tccctttccg atacgctgga agaggtgctt agctcatctg gcgagaagtc
     3481 gaaagaagag ttgagtaaga ttcgtagcaa agcggagcag gcactgaaac agagccgtta
     3541 tcgcctgggt gaaaccggtg atgccatcgc caaacaaacc cgtgtcgcgg cggcgcgtgc
     3601 tgatgagtat gtgcgtgaaa atccgtggac cggcgtggga attggcgctg caatcggtgt
     3661 agtgctcggc gttctgctgt cgcgtcgtta attatggcgg acactcatca cgcgcaaggg
     3721 cccggtaaaa gcgttctggg catcgggcag cgaattgttt ccatcatggt tgaaatggtg
     3781 gaaacacgtc tgcggctggc ggtggtggag ctggaagagg aaaaagctaa tctctttcaa
     3841 cttttactga tgctgggcct gacgatgctt ttcgctgcat ttggtcttat gagcctgatg
     3901 gtgctaatta tttgggcggt tgatccgcaa tatcgcctga atgcgatgat tgccaccacc
     3961 gtggtgttgc tgctactggc actgattggc ggtatctgga cgttacgtaa atcgcgtaaa
     4021 tccaccttgc tgcgccatac acgccatgag ctggcaaacg atcggcaact gctcgaggag
     4081 gagtcccgtg agcagtaaag tcgaacgtga acgacgtaag gcgcaactgc ttagccagat
     4141 ccagcaacaa cggctggatc tttccgccag tcgtcgtgaa tggctggagg caacaggcgc
     4201 ttacgatcgt cgctggaata tgctgctaag tctgcgctcc tgggcgctgg ttggcagtag
     4261 cgtgatggcg atctggacga ttcgccatcc taatatgctg gtccgctggg ccagacgcgg
     4321 ttttggcgta tggagcgcct ggcgtctggt taaaacgacc ctcaagcagc aacagcttcg
     4381 cggttaactt ccctctggcc ggagcaattc cggccttatc cctcaaattt tttgaagatt
     4441 tttgacagtt ttccttgcta acaatcatca ttcaccacgt ttatgattct ctccatcgac
     4501 agcaacgacg ctaataccgc gcccttgcac aaaaaaacaa tcagcagcct gagtggcgca
     4561 gtggagagta tgatgaaaaa attagaagat gttggtgtac tggtagcgcg cattttaatg
     4621 ccgattctgt ttattaccgc aggctgggga aaaatcaccg cttactctgg tacccaacaa
     4681 tatatggaag caatgggtgt cccgggtttt atgctgccgc tggtgattct gcttgagttc
     4741 ggtggtggtc tggcaatcct gttcggtttc ctgactcgca ccacagccct gtttactgcg
     4801 ggctttacgc tgctgacggc atttttattt cacagcaact ttgctgaagg cgtcaactcg
     4861 ctgatgttca tgaaaaacct gacaatttct ggcggattcc tgctgctggc aattaccggt
     4921 ccgggcgcgt atagcatcga ccgcctgctg aataaaaagt ggtaagcacg cgctatactt
     4981 aacgataaaa aacgaggagg aagctcctcg tttttgctat tggaggagag aaaaatgggt
     5041 caactgattg acggcgtctg gcatgacacc tggtacgata ccaaatctac cggcggtaaa
     5101 tttcaacgtt cagcttccgc atttcgtaac tggctcactg ccgatggcgc acctggcccc
     5161 actggcacag gcggttttat cgcagagaaa gatcgttatc atctctatat ttcactcgcc
     5221 tgcccgtggg cgcaccgcac gctgatcatg cgcaaactca aaggactgga accgtttatt
     5281 tccgtttccg tagtgaaccc gctgatgctg gaaaacggct ggacctttga tgacagtttt
     5341 ccgggagcaa ccggagatac gctctatcaa catgaatttc tgtatcagct ttatctccac
     5401 gccgatccac actacagcgg acgagttact gttcccgtac tgtgggacaa aaagaaccac
     5461 accatcgtca gcaacgaatc aacagaaatc atccgcatgt tcaataccgc gtttgatgcg
     5521 ctgggcgcga aagcgggtga ttactaccca ccagccctgc aaacgaaaat tgacgaactt
     5581 aacggctgga tttatgacac cgttaacaac ggcgtgtata aagctggttt tgccaccagc
     5641 cagcaagcct acgacgaggc ggtggcgaaa gtgtttgaat cgctggcgcg actggagcag
     5701 attttaggcc agcatcgtta cctgaccggc aaccagctaa ccgaagccga tattcgcctg
     5761 tggactacgc tggtgcgttt tgatccagtg tatgtgaccc acttcaagtg tgataagcac
     5821 cgcatcagcg attacctgaa tctgtatggc ttcctgcgcg atatctacca gatgccgggg
     5881 atcgccgaaa ctgtcaattt cgatcacatc cgtaatcatt acttccgcag ccataagacc
     5941 atcaacccta cggggattat ttcaattggt ccgtggcagg atctcgatga accgcaggac
     6001 gagatgttcg cttcggttaa aaaaaggcac cttacgaagt gcctctgata attagctaag
     6061 tgcatgttgt tcatatcgga tgcggcgtga acgccttatc aaacctacaa aacatttata
     6121 ttcaatatgt tgcaatgacc gcgcaggcca gataagcgta gcgcatctgg cgattttgcg
     6181 cttgtcatca gtcttagcgc gccttcacag cactttatta attccttgaa cgaatattta
     6241 ctgccatttc attcacgtct attcttaatt tgctgcttta aaaacaagtg attgagcaac
     6301 attgaggcaa aaatggactg gtatctgaaa gtaataaaaa attatgtcgg tttccggggt
     6361 cgtgcgcggc gcaaagagta ctggatgttt attctggtca acatcatctt tacgttcgtg
     6421 ctggggctgc tggataaaat gttaggctgg caacgcgctg gtggcgaagg catcctgacg
     6481 acaatctacg gtattctggt gtttttaccg tggtgggcag ttcagttccg ccgcctgcac
     6541 gacaccgacc gctcggcgtg gtgggcactg ctattcttaa tcccgtttat cggctggctt
     6601 attatcatcg tctttaactg ccaggcgggt acgccaggcg aaaaccgctt tggacctgat
     6661 ccaaaactgg aaccttaaaa caaatctgct cgccggatgt ctgactcatc cggcttttat
     6721 catcatgttg atgaatgaat aatttactaa tgtttattta aaatatttca atgtcgttat
     6781 ataaattacc tataaaaaat aaccatgtat ttatcttaaa tatcaaaacc tttataacat
     6841 ccgaaaaaga aagatacgaa tagcatttac acaatacccg gaatagactt tcgtcacttt
     6901 taattaaagg gatgttttta tgcagtggta tttatccgta ttaaaaaatt atgttggttt
     6961 ctctggtcgc gcacgtcgta aagagtactg gatgtttact ctgattaacg ccatcgtcgg
     7021 cgctattatc aatgtcattc aattgatttt aggtctggag cttccatatc tgtctatgct
     7081 ttatttgctg gcaaccttcc ttcccgtcct tgcgcttgct atccgtcgct tacacgatac
     7141 cgaccgttca ggtgcctggg ccttgctctt ttttgttccg tttattggct ggctcgtgct
     7201 tctggttttt ttctgcacag aaggtacttc tggcagcaat cgttacggaa acgatccgaa
     7261 gtttggttca aattaatctt agaattgggg cgatatttcg ccccttttta ttaacaataa
     7321 taaatattat tttccgctaa aaagtttggg aatttcccgc agacaccagg atttcgcttc
     7381 ccccatactg tcacgacgcc aggccataat aatatcgatc tcactggtcg attccgggct
     7441 gacgacacgc aaccgccctt ccgcaatatc tttttcgacc atcggatacg gcatcgtcgc
     7501 cacgccaagc cccgccagta atgcctgacg tttatcttca atcgtgctca ccgttaagcg
     7561 cggctgtttg tccagcagtt gtacggtcaa caccgggcgc tcacgagcgg tatccgctac
     7621 cgcaattcca cgatatttca cgcgcgtcac ttcagataac ggttccggct cctgatgaat
     7681 cgggtgatca ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc gcgagttgat
     7741 ctccgacgag gaacgaaaat gcatatccgg cgcgataaca atatccgccc gcccctgctc
     7801 cagtcgttcc tacgcccccg ccagcacttc ggtgatgatt gccagttggg tattggcttt
     7861 tgccgccagt ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg cttcggtcac
     7921 aatggtgaga tgcgtttccc aaccgcgcgc gagggcttcc gcatcggtag tcagtttatc
     7981 tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc ctcccgacat tggtgaattt
     8041 ggtgcgatgg cccgagcggt caaacagcac cacatccagc tcttcttcca gtttttgcat
     8101 ggtgtagcta agtgcggaag gcacgcgtcc cagctcatcc gccgccgccg caaaactgcc
     8161 ccggcgatcg atcgcatcca taacccgtag tgcttccagc gttaatgccc tttctttggc
     8221 catttcgttc tcattcagga aatttgaaca taccgggcag aatatctggc taacaatgta
     8281 gcgtccagcc ccttaccata aaaggaagta aagagaggtc aagaattatg attactaccc
     8341 gaactgccag gcagtgtgga caagcagact acggatggtt gcaggcccgg tatacttttt
     8401 cctttggaca ctacttcgac ccgaaattgt taggctatgc ctccctgcgt gtgcttaacc
     8461 aggaagtgct ggccccaggt gccgcctttc agccgcgaac ctatcccaaa gtcgatattt
     8521 taaatgtgat tctggatgga gaagcagagt atcgcgacag cgaaggcaat catgttcagg
     8581 ccagcgccgg tgaggcgttg ctgctctcta cccagccggg tgtcagctac agcgaacaca
     8641 atctcagcaa agacaaaccg ttaacacgaa tgcagctttg gctggacgcc tgcccgcagc
     8701 gagagaatcc gctgattcaa aaactggcgc ttaatatggg caagcagcaa ttaatcgcct
     8761 cgccagaggg tacgatggga agcctgcaat tacgccagca agtgtggctg caccatatcg
     8821 tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat
     8881 cgattcacgg gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg ctgacctgcg
     8941 gtgatggggc gtttattcgt gacgaggcta acattacgct ggttgccgat tccccactgc
     9001 gcgctttgct gatagatttg cctgtctagt tgtttttaca gggagatgat gatgagtaaa
     9061 aaatcggcca aaaagcgcca gccagtgaag cccgtggtgg cgaaagaacc tgctcgcacc
     9121 gccaaaaatt ttggctatga agagatgttg agcgagctgg aagccatcgt cgcggatgct
     9181 gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac aggttgccgg atgcggcgaa
     9241 aacgccttat ccggcctaca aaatccaaca acattaacga attaaacaac ccgtaggccg
     9301 gataaggtgc gccagcatcg catccggcac aatactcaaa acctggcgtt tatctggcct
     9361 tgctcgccat aatctcgata atctgccgat ccgtttgctg catcgaatgg cttgctaacg
     9421 cacacaggtt ggcaatcgac tgctcaacat catgcgccac gatcccttca ttgccggtca
     9481 cggcggtatc atccagcgcc attaacaccg ctttccacgc agccgaagca ctggtcgaaa
     9541 ccttcatcgc gcagctgttc gacgcaccat cgcaaatcat gccgctgaca tcgccgatca
     9601 tactgctgat cgccatcgag atagtttcat aacgcccatc caccagccat gccatcccgg
     9661 cggcggcccc cattgctgcg gtcgttgcgg cacaaagtgc agacaaacgc ggtaactggt
     9721 tatggatgta aattgcgctc aaatgagaaa gcatcagcgc acgcgccagt cgttcatcat
     9781 ccgctccgaa gtgttctgct acaaccacca caggcattgt tgcggtgatc ccctggttac
     9841 ccgagccgga gttactcata gccggaagcg tagcgccgcc catacgcgca tcggatgccg
     9901 cgctggtacg aatcacaatg gatgaagaga gatctttcgc cagcaaaccg cgctcgcact
     9961 gtttttccag cgtcgcgcca atatgcagcc cccacttacc gctcaaacct tcctgcgata
    10021 acgcacaatt tagcttcgcg gaatcgagaa taaagcggat cgccgcaaac gggacttcat
    10081 tgacgaactt caggatctca gccagcgtcg ttctggaaag caccgtcagc ggagactctt
    10141 gctcgccctc tgccacacac gcctgctggg taaacaccac accattgtgc gtctcaatac
    10201 gcacaatgtt ggtatgcccg ccgacgatgg tgacacacgc ccacttctca ccgttccaga
    10261 ctttggcgcg tgagaagagg atttcattgc aaggttcctg gatcttaacg gagactttcc
    10321 ccgccgccag cagtgctttg gcatcggcaa ttgcctgcgc agttgcgtct ttcagcactt
    10381 ccagcccggc gttggcattt ccacctaacg cccccagcgc cgccgcaatc ggcagcccca
    10441 ccattcccgt gccgggaacg gtgacgccca gaccgttctt catcagattt ggcgaaaccc
    10501 aggcttctac acgttcaacc ggaccttcca gttctgccgc agcaaccgcc gccgccagcg
    10561 ccagtgaaat cggttcagta catcccagcg ccggttttac ttcctcctga acggcgagga
    10621 tgtaacgctg ccataacgga tttaaagtcg aatcaaacat attaaaaacc ttaaaatttc
    10681 aggtaaatca agaaaatgcc aggaacggag aaacacaaag caacaaacca gtgacgataa
    10741 tcaggtacag agacatccct ttgtatttgt gcaatgccgg tactttgtaa accagccacg
    10801 ccgggatcag gcaccctacc atgccgaaaa tcgggctaca gatagaggtg aagctcaaca
    10861 ccggtgcgtt cagtacgata gcgctccagg ccagcaaaat ggcgaaaatc atgatgccgc
    10921 gctgaacaag attttcgtta atcttctcgg caggcatctt gcgacgcagg atgttcatta
    10981 cgatcccttg cgttgcttcg cgaaagccta aatagacgcc aaagaatgca gtcatgacag
    11041 caaagatatt gagaatgacg ctgaccactt tcacccacgc tgcgccgtca ccgctaataa
    11101 actgtgcggc aatcgccagc gcggaaatat tctgctcgta ggcttttacc gcttcgtcat
    11161 gccccatcgc cagcgtgaac gacacggcgt agaaaaagac ggtgacaaac aaaatgccaa
    11221 acgcgatatt cattgcccgc aatgctttat gccgcgccac ttcaatagat ttttcccgcg
    11281 agcgataaga gatcaccatc gggcttaacg tctggataaa caaaatcgac gtcagggtaa
    11341 acggcagcgt aataatggcg tttttcacca gcagccccag cggcggtagc gaaccgacgt
    11401 tatacagatg ccacattccc accatcgaca cgcccagcgc cgcgaccacc agcagcttgg
    11461 tcagcaccat gccggtcgaa attttgaata acaatttctc gccgcgtgaa gagatcgcca
    11521 ccagaatgca aatcagtacc agaccataaa agggactgtc tgacagcaac ccttccgtca
    11581 cgccgaaggt atgcagatag gaagcactat cgttggtgat ggcggtggaa tagacgaaca
    11641 tccaaatcac cagcattacg aaatagagcg cacctaacag gatgccccag tttttaccta
    11701 aataaccgct aatgacactc gggtaatctt tacattctgg tgattctgcc agcgtattaa
    11761 taaacaaccg ctgaaacaga tacattgccg ggtaaccaat caccgatgag agcaaaaata
    11821 cccacaatcc catcaaaccg acctgcaccg ggagaaaaac aatccccgcg ccaatcgcca
    11881 tcccgatact cataatcacc cagccggtgt cagtactgtc gaatttgatc gcttctcgcc
    11941 actcgctctc actcattcca gcacgacccg ccggggtcga agcgtctgca atgacgcctt
    12001 tattcgatgc gatttccata atttctcgct caatattttg tagggcttat tatttttttc
    12061 cgagccgcat caaggcgata tgcggtctac gtttttgcag gcgagttatt agaataaaga
    12121 aatgatacgc gcgaggtcag agaaaatctt cacaatcaag actttgaaat aacggagatg
    12181 gataagaatt ttctactaaa ttaatcgcaa gagaatattc attctctatt tgcggcagat
    12241 cacaaaaaaa aggtgcacat ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg
    12301 cagaccactt taatggccag tcctccgcgt gatgtttcgc ggtatttatc gttcatatct
    12361 ttgccggttt catacatcgt ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg
    12421 cggcgcatcg ccatccacgc ggcgtttact gctttcacgg cattaatggc attacgttca
    12481 atgcacggga tttgtacctg tccggcaacc ggatcgcagg tcagcccaag gttatgctcc
    12541 atcgcgattt ccgccgcatt gcatacctgc gccggactgc cgcccagtag ttcagttaac
    12601 cctgccgccg ccattgaaca ggccacgcca atctccccct gacagccgac ctccgcgcca
    12661 gagatggagg cgttcatttt atacagcgcg ccaatagccc ccgctgccag aaaatagcgg
    12721 gcaattgacc gctcgtttac cggacgacgg aacttatcgt aataagccag cactgccgga
    12781 ataatgccgc acgcaccgtt agtcggtgcc gttaccacgc gcccgccagc tgcgttttct
    12841 tcactaaccg ccagcgcgta catgttgatc cagtcgatga cattcatcgg atcgttagag
    12901 atgttatcgc tagaaaccag ctgacgacgc agcgctacgg cacgacgcgg cacattgagc
    12961 ggaccaggca gcacgccttc agtgttcatc ccgcgttcaa taccgtcatg catcacttgc
    13021 cagatacggg caaatcctgc gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc
    13081 atcatcaggc cagatataga caggccgttg taatcacaca ttttcagcag ttcacctgct
    13141 gagtggaaat cgtaaggtac ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct
    13201 tcgacaataa acccgccgcc gacagagtaa taggttttac ttaatagcgc ttcctgtccc
    13261 ttccaggcag tgatccgcat tccgttctca tggcgaggca acatttcggg atggaagata
    13321 atgttctttg ctacaggaaa atcaacaata tgcgcaccag atgccactgg cagccgcccg
    13381 ctgcgcgtta ctaactctat aaatgcaggg atctcatcaa tgacaacatc ctgcggactg
    13441 tttcctgcca gtcccatgat aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac
    13501 aacgacccat acagatcgac cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc
    13561 agccgatcaa taaaactttt tccggcattc attggcccca cggtatgcga actggaggga
    13621 ccaatcccaa ttttgaaaat atcgaatgca ctaatcatat ccacaccctc ggattgccgt
    13681 tcagtgaagt ggagcggaac gaccttgcga ccgtcccgct cacgaggctt tacgcactac
    13741 gtactgcgat agcttcaatt tccagcttca catctttcgg caaacgcgca acctgcacac
    13801 agctccgggt cgggtaggtc gcctgatgct catcgaagaa ctgcttatag acttcattga
    13861 tggtggcaaa atcattcaga tcggtgatga acacggtcat cttgatgata tcgcccacgc
    13921 tcagcccggc agcaaccacg atcgctttga cgttttcgag gcttaaacac gcctgatctt
    13981 gcacatcagc cgggatctca ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga
    14041 ccatgctgcc taaatcaacg ccctgaacat aagggccgat tgcgcctggg gcacgttgcg
    14101 tttcgataat ctttttcata catcctccgg cgtcagagcg cctgggtaaa agtacgtgaa
    14161 ataacatcct gttgctgttc acgggtcagt gcgttgaagc gcacggcgta gccagagaca
    14221 cggattgtca ggttaggata tttttccggg tgctcgatgg catccagcag catttcccga
    14281 ttcattacgt tgacgttgag gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac
    14341 ccatccagca ggccgacaag gttggtttta cgtactggat cttctttgcc cagcgccgca
    14401 ggaacgattg agaaggtgta cgagatccca tctttggcgt aggtgaacgg cagtttcgcc
    14461 accgacgtca atgaggccac ggcacctttg cggtcacgac catgcatcgg gttagcgccc
    14521 ggcgcgaacg gtgttccggc gcgacgaccg tccggcgtat taccggtttt ctggccgtac
    14581 accacgttag aagtgatagt cagaatcgac tgggtaggga cggcgttgcg ataggttggc
    14641 agcgctttaa ttttcttcat aaagcgttca accaggtcgc aggcaatgct gtctacgcgc
    14701 tcgtcgttgt tgccgtactg cggatattca ccgtcgattt caaagtccac cgccaggccg
    14761 ttttcgtcac ggattggttt cacgcgggca tatttgatgg cagacaggga gtccgtcgcc
    14821 accgacaggc ccgcgatgcc gcatgccata gtgcgataga catcacgatc gtgcagcgcc
    14881 atcagcgaag cttcgtagct gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg
    14941 atgtactgca ccgccagcca gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa
    15001 tccagcacgt cgtccatcag cggtgctgtt ttcggcccga cctgaatctt cagcttctcg
    15061 tccaccccgc cgttaattgc gtacagcagt gttttcgcca ggttagcgcg tgcaccaaag
    15121 aactgcattt gcttaccaat caccattggg ctgacgcaac aagcaattgc gtaatcgtcg
    15181 ctgttgaagt cagtacgcat cagatcgtcg ttttcatact gcaaggaaga ggtgacgatc
    15241 gacacctgcg cggcatattt tttgaaggca atcggtaatt cttccgacca aagaatggtc
    15301 aggttaggtt ccggtgccgg ccccatagtg tgcagggtgt gcaaatagcg gaaggagttt
    15361 ttggtcacca gcgtacgacc gtccagcccc atcccgccga tcacttccgt cgcccagatt
    15421 gggtcgccgg agaacagcga atcaaattcc ggcgtacgca agaagcgcac catacggatc
    15481 ttcataatga agtgatcgat cagttcctgt gcctgctgct cattgagtac gcctgcttta
    15541 aagtcgcgct caatgtagat atcgaggaac gacgccgtgc gccccagcga catcgcgccg
    15601 ccgttctgtg atttcactgc cgccagataa gcgaagtaga gccactgcac cgcttcctgc
    15661 gcattctgcg ccgggcgaga gatatcaaag ccatatttcg ccgccatttc ctgaatctgc
    15721 aacagcgcat gacgatgctc tgccagctcc tcacgcagac ggatggtggc ttccagatct
    15781 tcgccttttt ccagacggga ctggagatcg gcaaattgca gttcgcgttc acgcaccaga
    15841 taactgatgc catacagcgc tacgcggcga tagtcaccga taatgcgccc acggccatag
    15901 ccatctggta aaccggtcag cacgccagat ttacggcagc gcagcatatc cggtgagtaa
    15961 acatcaaata cgccctggtt atgggtttta cgcagatcgg taaacagata ttcaaattca
    16021 ctgtccattt cacggccata ggcgtggaat gaacttttaa tcatattaat gccaccgaac
    16081 ggatgcagcg cgcgctttaa tggcgcatcc gtttgcagac caacaatttt ttccagcggc
    16141 tggttaatat atcccgcatc atgagcggta attgtggtgg caatattggt atcgaaatca
    16201 accggcgcgt gggttgcgtt ttcgatacgg atgccttcca ttactttttc ccacaattcc
    16261 gtggtggcag gcgtcgcttc ggcgaggaaa gattcatcgc cttcatacgg tgtatagtta
    16321 tgttgaataa aatcgcggac attaatttcg tttttccagt ccgtaccttt aaagccaagc
    16381 catgcgtcgg cgtacagctt atcgctggta tcaatatcta tcttcatgaa aaataatctc
    16441 tctacaatac ttcaactaaa ttatgcaaat tctgcgggcg cgttaacttt gcctaaatga
    16501 atggcatcca aagcaatcat tttttcttcg ttagtcggaa taacggcaca aatgacacgc
    16561 gcattttcac tggaaacaat tcgctctcca aaagagttag agcgattatt catttctgta
    16621 tcaatcacta cgcctaatac agccaaatgt tccatgacca gacgacgaat taaacttgaa
    16681 ttctctccta ttccgccggt gaatataatt ccatccaggc gacgtaatga agctgcgtgt
    16741 ccggcaatat gacgggcaat tcggtgaaca aaggttttaa ttgccagttg cgcgcgttcg
    16801 tgaccttcat gccaggcttt ttccagaaca cgtaaatccg aagaaagacc ggaaatacct
    16861 aataatcccg actctttatt cactacgcgt tccaggtcac ccaggctctg gttggtttgg
    16921 ctggcgaccc aggacatcgc accaaagtcg acatcgccac tgcgggttcc catcatcaag
    16981 ccttccagcg gcgtcattcc cattgaagta tcaacactct gaccgttgcg aaccgcgcag
    17041 attgacgcgc cattgccaag atgcgccaca accaggccgg agtcatcttc cgccagattc
    17101 agcagagaat gtgcgcgctg ggaaacatag cggtgcgacg tgccgtggaa accataacgg
    17161 cgtacaccta actcttcata atatttccac ggcaggccgt ataaataagc ttccggagcc
    17221 atcgtctggt ggaaactggt atcaaatacc gccacctgag ttacgcccgg aaataattgc
    17281 tgcgccgatt caataccact taaattggcg taattatgca ggggtgccag tggagaaacg
    17341 cgacggatat tatcaatgac ttcatcggta ataatggcgg actcggtaaa aatactgccg
    17401 ccgtgagcga tgcggtggcc aattaaggcc acactgtcat ttaaattccg tttttccagt
    17461 tcaaatgcaa ttgccttcaa tgcaccttcg tagctgtggt gagccagcgg tgctggctct
    17521 cccccattta cggataagaa tgcattttcc gagttaatac cgtcggcaat acctgacatt
    17581 aatacttcac agtcgctggc atcgagcacg gaaaacttaa tcgaagacga accacagtta
    17641 ataaccaaaa caaccggaaa ttcattcatc tcttttctca tcctgagtta cggattaaaa
    17701 cagtttgtat acgatgttca ggatggtcag cagaccaatc acggtaacaa acacgttatc
    17761 cagacgacca cggtatttcg ccagagacgg cgctttacgg atggcataca tcggcaacag
    17821 gcacagcagg gatgcgataa tcggtgcgcc catggcttca atcaggtcga ggatgttcgg
    17881 gttggcgtag gcaacaaccc aggtggagcc catgatgaag atcatgctga tagtgttcag
    17941 tttacccagc gacactttag ttttgtcgcc tttataacca aacttcagga ccaggccatt
    18001 cagaccttcc agcgttccca gatagtgacc gaagaaagat ttgaagatag ccacgagtgc
    18061 gatgatggaa gccgcatatt ccagtgtaat cgcgaacgtt gttttggtac cggtcatgga
    18121 cgcaaagtgg ttagccagat aagaaagcac tggaatattc tgcgctttgg cttccgccat
    18181 gttggccgga gacagcgtaa ataggcagct aaaggcaaag aacatcacca ctgcaaccat
    18241 cagcatgctg gcacgagaaa tgatttggga acatttacgt tcggtgaagt cgcgaccgaa
    18301 gtctttctca tactcttcac gtttagaaac cacgaaggaa gagacgattg gcgagaaatt
    18361 aaaggagaaa accatgatgg aaatccccag ccagacggtg atcaggatac cgtcatgacc
    18421 ggttaacgac agcgaaccga ggtcaacctg gtcgataact gcagagttcc agtaagggat
    18481 cagcgacaga gaaatcagca ccaggctggc gataaacggc cataccaggt agctcatcac
    18541 tttaaccatc agatccttac caaaccagat gacgaaagcc atcagcagca gcaggaacag
    18601 cgccacaaag ccgcgattca gcggtgcaaa gccgagctgg ttttcccaga acgtcataaa
    18661 ggtattggta atagtaacgc cataaatcca cagcagtggg caaatcgcga agaagtacag
    18721 gaacgtgata accacgccgc cagttttacc aaaatgctct tccaccgttt ccgtaatgtt
    18781 gccggaaggg ttagagccag aaagacatag acgcgccagc gcccggtggc aataaaatgc
    18841 gatggggtat gccaatacca acatcagaag aatcgggatc agtccgccaa aacctgcgcg
    18901 gatagggaag aacagcaccc cggcgccgat tgccgtacca aacaagccta acgtccatgt
    18961 ggtatctgat ttacgccagg acgattgttt tgtctggctg gatacaatgc tatctgaagt
    19021 actcatatcc tatcctcaac gaattaatta agcgtcaacg aaaccggtga tttgagagac
    19081 gcgagaaaga tcgatattgc cgccggaaat aatactgacg gtttttctgt tttgaatata
    19141 ttggtctaat ttaccgctta ataatgcagc acatgccaga gcgcctgcgc cttcggtgac
    19201 gactttattg cgctgaatta aggcaatcat actgtttctg atttcgtctt cgctgaccag
    19261 cacgatgtca tcgactaatt cacgaacgat ttcgtaagtt aaattacccg ggcgggagac
    19321 atcacaacca tccgccaggg tgccggtagt tcggtgcgtg gttatttctc cggagtggaa
    19381 agaagccgcc atgccgtgaa cgttttcaga ctgtacgcca ataacacgaa tggtcgggtt
    19441 aatagattta attgccaccg caataccagc aattaaaccg ccaccaccaa ttggcacaat
    19501 cacgttatcg acatcataga gatcttccat aatttccaga ccaatcgttc cctggccagc
    19561 aatcactttc ggatcatcgt aaggtgggat aaaaatacgg ccttccattt cgacaatttc
    19621 gctcacttta gcgatagtgt cgttgaagtt atcaccatgc agaacgactt ctgcggagta
    19681 gtcgcacgtt gccgctactt tggattttgg cgcacctttt ggcatcacca ctttaccgtc
    19741 gatacccagc atcgcgcagg agagcgaaac cccttgcgca tggttgcccg cagaacaggc
    19801 caccacgcct ttgcgttttt ccgcatcggt cagtgaactt aatttattaa atgcgccacg
    19861 aattttaaat gaacccgtac gctgcatatt ttcaaacttc aggaatattt cacctttgca
    19921 acgttcacta aaatagttgg agcgaggcat gcctgtttta taaattcgcc cagccagtcg
    19981 ttgtttcgct tcaataatat catcaatagc aatcggcaga tcgtatgtaa tatgcattat
    20041 aaaacctctt cgcctgaatt aaatatgtag gtaaccgtaa ccgacacctg caaaacagac
    20101 aggtggatta tttatgttta aataaaaaca aataactaac caacttctat taattgcctt
    20161 cgtctacacc cattataaga tgaatactct ttggctaatt ccaccaaaac cgatgctgct
    20221 tttttaatac gataattttt cgaccatacc gcggcatatt gtgccacagg taatgtttct
    20281 tcaaccggaa tagtaataaa ttgattagaa ccaaaaggtg acgtcatatc acaaggaatt
    20341 acagttaaga aatcagcatt gagaacaaga ttataaattg tcacgactga gtcggtttta
    20401 acgatgtttt caatactgat gccatttctt tgtaacgtag taagcagttc gctgtagtac
    20461 cccatattag tttgtggcaa cacccactgt tcgttcttca acgactccag cgtggtggtg
    20521 ccggtgcatg ttcgggactt actggctacc agcacaaact cggactcgaa cagcggctca
    20581 acatgtaaat cctgaagctt catttctgca cttaatgtac caatcgcaaa atccagtcga
    20641 ccgtcgcgga ttgccggtaa gaacgaagac agttgcgctt catacataga aacctgcgct
    20701 ttcgggaaca cctctttgaa cttgttgatc atccctgaca taaaagtaaa accaatcaat
    20761 gaaggaaaac caaatgagac ttccaccacc gcctcagaag acataccgct tatctcatta
    20821 accatatttt tcatttcacg ggtaatggat tcggaacggg agagtaacaa ttgaccggca
    20881 ggtgttaatg ttacaccggt atttttccgc accactaatt ccacaccaaa ataatcttca
    20941 atatcgttaa tgattttact gacggccggt tgagttaacc ctaattcttt tgcagccgag
    21001 ccgatagagc cacttctaat gacttcctga aagactacca ggtgctgcgt tttcggaaga
    21061 agaatagtgc tcataacgac ctacgttaat tacctcattg acggcatgaa gtgtatcaaa
    21121 atgaaatgaa caggatatgt gcgaccactc acaaattaac tttcaatact ttccagggta
    21181 tcgtcattac aattaaatac ctttaaatat caacaagtta aagcataaaa atcagcataa
    21241 accctgattt ttgtcaaaaa aaatgacggg gataaccata ttttatggcg ataacatcat
    21301 tcgttatgtg gttaatacaa aaaaggggct gagaccatat ttcaagcaat taccggataa
    21361 ttaccaggca aaatttgagt taaatttaat gagcaagttc tcaaatttta ttataaataa
    21421 accattttca gcgataaata ctgcggcacg tcacattttt tcacgctatt tgttggagaa
    21481 caaacattta ttttatcaat attttaaaat ttcgaataca ggtattgatc atctcgaaca
    21541 attgattaac gtcaactttt tctcttctga caggacgtca ttttgtgaat gcaatcgttt
    21601 tccataaatt cttctcccct cataggcgac gaatagcatt ttgtgttgag gatcacaaaa
    21661 cgaataattg ctgatcgccg cgataaggtc agacaaagac aacaagggaa attttcacag
    21721 agcttttgat cgtcgtaggc cacagaatgt tgcttcgttt actgtaacgc cgggtaaatg
    21781 agcgtttttt gatagtgcga aagaaccctg cgaggaaaat aagcatctat tattgtttgt
    21841 catgacgaca aggcaggcat caatgaaagg cttcccaatt gcacatattt ttcacccttc
    21901 aatcccgcca atgcacgcag tggttaacaa tcacaataga aatattgatt attggacggt
    21961 aaaaagaaag tttgcag
//
LOCUS       DAJTVJ010000068        21719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000068 DAJTVJ010000000
VERSION     DAJTVJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21719
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.068"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OO448_002496"
     CDS             complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OO448_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR6337683.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OO448_002497"
     CDS             complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OO448_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR6337684.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OO448_002498"
     CDS             complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OO448_002498"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6337685.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4042..4800
                     /gene="loiP"
                     /locus_tag="OO448_002499"
                     /gene_synonym="yggG"
     CDS             4042..4800
                     /gene="loiP"
                     /locus_tag="OO448_002499"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6337686.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OO448_002500"
     CDS             complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OO448_002500"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6337687.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OO448_002501"
     CDS             complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OO448_002501"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6337688.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OO448_002502"
     CDS             complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OO448_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6337689.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8253..8468
                     /locus_tag="OO448_002503"
     CDS             8253..8468
                     /locus_tag="OO448_002503"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337690.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8772..9926
                     /gene="metK"
                     /locus_tag="OO448_002504"
     CDS             8772..9926
                     /gene="metK"
                     /locus_tag="OO448_002504"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6337691.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10362..11756
                     /gene="galP"
                     /locus_tag="OO448_002505"
     CDS             10362..11756
                     /gene="galP"
                     /locus_tag="OO448_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6337692.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11833..12330
                     /locus_tag="OO448_002506"
     CDS             11833..12330
                     /locus_tag="OO448_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6337693.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12425..13132
                     /gene="endA"
                     /locus_tag="OO448_002507"
     CDS             12425..13132
                     /gene="endA"
                     /locus_tag="OO448_002507"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6337694.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13212..13943
                     /gene="rsmE"
                     /locus_tag="OO448_002508"
     CDS             13212..13943
                     /gene="rsmE"
                     /locus_tag="OO448_002508"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6337695.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13956..14906
                     /gene="gshB"
                     /locus_tag="OO448_002509"
     CDS             13956..14906
                     /gene="gshB"
                     /locus_tag="OO448_002509"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6337696.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15015..15578
                     /locus_tag="OO448_002510"
     CDS             15015..15578
                     /locus_tag="OO448_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6337697.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15578..15994
                     /gene="ruvX"
                     /locus_tag="OO448_002511"
     CDS             15578..15994
                     /gene="ruvX"
                     /locus_tag="OO448_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6337698.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16169..17149)
                     /locus_tag="OO448_002512"
     CDS             complement(16169..17149)
                     /locus_tag="OO448_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6337699.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17167..17871
                     /gene="yggS"
                     /locus_tag="OO448_002513"
     CDS             17167..17871
                     /gene="yggS"
                     /locus_tag="OO448_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6337700.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17889..18455
                     /gene="yggT"
                     /locus_tag="OO448_002514"
     CDS             17889..18455
                     /gene="yggT"
                     /locus_tag="OO448_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6337701.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18452..18742
                     /locus_tag="OO448_002515"
     CDS             18452..18742
                     /locus_tag="OO448_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6337702.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18750..19343
                     /locus_tag="OO448_002516"
     CDS             18750..19343
                     /locus_tag="OO448_002516"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6337703.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19336..20472
                     /gene="hemW"
                     /locus_tag="OO448_002517"
     CDS             19336..20472
                     /gene="hemW"
                     /locus_tag="OO448_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6337704.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20783..21719
                     /locus_tag="OO448_002518"
                     /pseudo
     CDS             20783..21719
                     /locus_tag="OO448_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         5379 a         5651 c         5530 g         5159 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa
      541 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
      601 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca
      661 gcccaccgag cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga
      721 tcacaccaat agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac
      781 caccggtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
      841 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
      901 caataaaagc accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga
      961 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
     1021 cgcggctgat tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta
     1081 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
     1141 cggcagcaga aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac
     1201 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
     1261 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
     1321 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
     1381 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
     1441 aatagtcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
     1501 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa
     1561 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
     1621 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
     1681 tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
     1741 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg
     1801 acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg
     1861 aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca
     1921 atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
     1981 gccgcatcct gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg
     2041 ccttcggcag tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg
     2101 atgaagatca gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg
     2161 tttgccagtt gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc
     2221 gcggtcggac cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
     2281 tcacacggac gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc
     2341 ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
     2401 tgacgctgtt tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc
     2461 aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg
     2521 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
     2581 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg
     2641 aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg
     2701 attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca
     2761 gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc
     2821 gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca
     2881 tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt
     2941 tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg
     3001 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
     3061 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg
     3121 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
     3181 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
     3241 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
     3301 tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
     3361 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
     3421 ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta
     3481 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
     3541 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
     3601 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
     3661 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
     3721 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
     3781 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
     3841 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
     3901 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
     3961 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
     4021 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
     4081 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
     4141 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
     4201 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
     4261 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg
     4321 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
     4381 gggctgatgg atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg
     4441 cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg
     4501 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt
     4561 gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat
     4621 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
     4681 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg
     4741 gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa
     4801 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg
     4861 cacatttgtt accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta
     4921 cgggtggcat cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct
     4981 ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca
     5041 cttccactac gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag
     5101 cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat
     5161 ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt
     5221 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
     5281 cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca
     5341 gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat
     5401 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
     5461 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg
     5521 cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa
     5581 aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
     5641 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac
     5701 cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc
     5761 agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
     5821 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
     5881 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
     5941 tcacaataaa ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac
     6001 ttactcatct tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg
     6061 ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag
     6121 cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga
     6181 cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc
     6241 gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag
     6301 catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc
     6361 atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg
     6421 acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
     6481 ccatgcgtcc ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg
     6541 ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg
     6601 cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa
     6661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
     6721 ttcacgcaaa gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
     6781 ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc
     6841 cacgccgatg atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc
     6901 ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat
     6961 gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt
     7021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
     7081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat
     7141 attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc
     7201 ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt
     7261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
     7321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat
     7381 cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat
     7441 atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
     7501 cagtactgcc atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg
     7561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta
     7621 atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa
     7681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
     7741 acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
     7801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt
     7861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
     7921 tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
     7981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
     8041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
     8101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
     8161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg
     8221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
     8281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
     8341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
     8401 ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
     8461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
     8521 tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
     8581 acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt
     8641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
     8701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
     8761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
     8821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
     8881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
     8941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg
     9001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg
     9061 gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
     9121 cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
     9181 cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
     9241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
     9301 aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
     9361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
     9421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga
     9481 tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac
     9541 gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct
     9601 acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa
     9661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg
     9721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc
     9781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
     9841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
     9901 tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc
     9961 gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa
    10021 agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact
    10081 actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc atattattat
    10141 tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt
    10201 taatctatga tgatataact caattatttt catgcactta aaccataact aagataaatg
    10261 ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct
    10321 atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac
    10381 aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat
    10441 tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat
    10501 tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag
    10561 tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga
    10621 tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag
    10681 tactgattct ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac
    10741 cgctgtacct ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc
    10801 agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca
    10861 ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg
    10921 gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg
    10981 ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg
    11041 aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca
    11101 acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga
    11161 tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca
    11221 cagagcaaat gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg
    11281 caatcggcct tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga
    11341 tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg
    11401 cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg
    11461 gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca
    11521 tcacctgctc cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga
    11581 ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac
    11641 tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata
    11701 ttgaacgtaa cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc
    11761 ctcaggcttc ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta
    11821 tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc
    11881 ggcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca
    11941 aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa
    12001 atccgcctca atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta
    12061 ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc
    12121 aaagagtgga agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag
    12181 ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat
    12241 cagcttaccg tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt
    12301 cactgcggtg aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc
    12361 tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta
    12421 agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc
    12481 ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca
    12541 cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg
    12601 cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg
    12661 cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca
    12721 ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat
    12781 gtataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag
    12841 ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga
    12901 aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat
    12961 gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa
    13021 caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca
    13081 aggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact
    13141 agcgggattc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg
    13201 gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc
    13261 acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg
    13321 ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg
    13381 ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc
    13441 cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc
    13501 agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg
    13561 ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg
    13621 ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg
    13681 aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta
    13741 acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag
    13801 gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt
    13861 tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag
    13921 tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg
    13981 accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac
    14041 agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg
    14101 aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt
    14161 tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc
    14221 cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag
    14281 ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg
    14341 cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag
    14401 cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt
    14461 cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg
    14521 agaatggcac tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca
    14581 aacgcgtgct ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg
    14641 ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg
    14701 aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt
    14761 ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct
    14821 gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca
    14881 tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct
    14941 gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa
    15001 cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg
    15061 atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg
    15121 ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga
    15181 agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg
    15241 gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca
    15301 gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg
    15361 gcaccgataa acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag
    15421 gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata
    15481 ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg
    15541 atattctcac catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt
    15601 cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg
    15661 ccctttgcct gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt
    15721 actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac
    15781 cgagcaacca ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg
    15841 tgttgaagta aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt
    15901 tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat
    15961 tattctcgaa agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt
    16021 cgttaccgcc atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga
    16081 ttgtaggtct gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg
    16141 ataagcttgc acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa
    16201 aactctgctg aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact
    16261 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
    16321 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
    16381 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
    16441 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
    16501 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
    16561 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
    16621 aaccaatttc ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat
    16681 cttccagcgt cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca
    16741 gcgtggtaga tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct
    16801 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag
    16861 gtaacagccg taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc
    16921 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
    16981 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg
    17041 cagcttccat tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat
    17101 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
    17161 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
    17221 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
    17281 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
    17341 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
    17401 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
    17461 tggtgtcata ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca
    17521 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
    17581 gggattcaac tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta
    17641 cgtctgcgcg ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa
    17701 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
    17761 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
    17821 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
    17881 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
    17941 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
    18001 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
    18061 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
    18121 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc
    18181 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
    18241 gggtaagcca gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc
    18301 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
    18361 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
    18421 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
    18481 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
    18541 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca
    18601 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
    18661 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
    18721 aatcgcggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
    18781 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
    18841 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
    18901 ttctgaaagc gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
    18961 gtctggcggt agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg
    19021 aagacgcgac cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg
    19081 acgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
    19141 cgactccgct ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca
    19201 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
    19261 aactgacccg cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc
    19321 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
    19381 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
    19441 cacgacgatt atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag
    19501 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
    19561 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
    19621 attactatgg aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt
    19681 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga
    19741 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
    19801 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
    19861 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
    19921 ctgaccatcg aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac
    19981 gcgctgtggg atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
    20041 tatgaaactt ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg
    20101 cgatttggtg actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat
    20161 gggcgcattc tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat
    20221 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    20281 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    20341 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    20401 gattactggc agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc
    20461 ctcgctgagt aatcattaat gcgcctgcgg gcgcattaat cacttctttt ccccccgtta
    20521 attaacttcg gcaaaatata tttcttactg aataaacatc gatgatattt attagatata
    20581 tctagtgaat atttactcat catttattga ttataaacat gtacaacctg gcgcacaaat
    20641 ttaatattta atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagata
    20701 ggttaaccaa tttgtcgcac aaagcactga taagcacaat tagatatcaa ccaatttcca
    20761 tccttcaaca gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc
    20821 atccttgctg gctctggtta gcactggcgt aaacgccgca gaaaaagtga cgttaaagtt
    20881 agcacacaac ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga
    20941 ggtaaaacag ctgtcagaag gtaatatggt gatccgtatt tatcccagca gccagatggg
    21001 taatgcgcga taaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc
    21061 cgccagtgat ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt
    21121 taaagatcag gctcacttca acaaagttgt ttttggtgaa gttggtaaag aaattatgga
    21181 ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta cacgcagttt
    21241 ctatgcgaaa aaaccgatta ctaagccaga agatcttaaa gggctgaaaa ttcgtgttca
    21301 gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca ctccaatttc
    21361 atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag aaaataacgt
    21421 gccttcctgg gtgcagactc gacatattga aattgccaaa gttttttctg aagatgaaca
    21481 cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat taaccccaga
    21541 acagaaacaa atcctcgaaa cagcagcgaa gaaatccgaa gcttatcaac aaaaattgtg
    21601 ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg aaattgtaaa
    21661 agtggataaa gcaccattcc gtgccgcagt tcagcctctg tttgacgact tcaaaaaag
//
LOCUS       DAJTVJ010000069        21643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000069 DAJTVJ010000000
VERSION     DAJTVJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.069"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OO448_002519"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OO448_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6337705.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="OO448_002520"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="OO448_002520"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6337706.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OO448_002521"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OO448_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6337707.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="OO448_002522"
     CDS             complement(3717..4511)
                     /locus_tag="OO448_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6337708.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="OO448_002523"
     CDS             4649..4990
                     /locus_tag="OO448_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6337709.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OO448_002524"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OO448_002524"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6337710.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="OO448_002525"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="OO448_002525"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6337711.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="OO448_002526"
     CDS             8906..10198
                     /locus_tag="OO448_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6337712.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="OO448_002527"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="OO448_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6337713.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="OO448_002528"
     CDS             complement(10731..11189)
                     /locus_tag="OO448_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6337714.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="OO448_002529"
     CDS             complement(11186..12103)
                     /locus_tag="OO448_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6337715.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="OO448_002530"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="OO448_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6337716.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="OO448_002531"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="OO448_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6337717.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OO448_002532"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OO448_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6337718.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OO448_002533"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OO448_002533"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6337719.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OO448_002534"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OO448_002534"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR6337720.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="OO448_002535"
     CDS             16063..16749
                     /locus_tag="OO448_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6337721.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="OO448_002536"
     CDS             16746..19160
                     /locus_tag="OO448_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6337722.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OO448_002537"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OO448_002537"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6337723.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OO448_002538"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OO448_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5799 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgaccaggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccaatg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg atggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttt aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAJTVJ010000070        21557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000070 DAJTVJ010000000
VERSION     DAJTVJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.070"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1366
                     /gene="espL1"
                     /locus_tag="OO448_002539"
                     /pseudo
     CDS             1..1366
                     /gene="espL1"
                     /locus_tag="OO448_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1538..1645
                     /locus_tag="OO448_002540"
     CDS             1538..1645
                     /locus_tag="OO448_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337724.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1698..2456)
                     /locus_tag="OO448_002541"
     CDS             complement(1698..2456)
                     /locus_tag="OO448_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6337725.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2743..3672
                     /gene="pfkB"
                     /locus_tag="OO448_002542"
     CDS             2743..3672
                     /gene="pfkB"
                     /locus_tag="OO448_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6337726.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3773..4063
                     /gene="ghoS"
                     /locus_tag="OO448_002543"
     CDS             3773..4063
                     /gene="ghoS"
                     /locus_tag="OO448_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6337727.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4169..5029
                     /locus_tag="OO448_002544"
     CDS             4169..5029
                     /locus_tag="OO448_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6337728.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5070..5606)
                     /locus_tag="OO448_002545"
     CDS             complement(5070..5606)
                     /locus_tag="OO448_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6337729.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5753..6421
                     /gene="hxpB"
                     /locus_tag="OO448_002546"
     CDS             5753..6421
                     /gene="hxpB"
                     /locus_tag="OO448_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6337730.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6584..7174
                     /locus_tag="OO448_002547"
     CDS             6584..7174
                     /locus_tag="OO448_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6337731.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7307..8698
                     /gene="tcyP"
                     /locus_tag="OO448_002548"
     CDS             7307..8698
                     /gene="tcyP"
                     /locus_tag="OO448_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6337732.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8702..9518)
                     /locus_tag="OO448_002549"
                     /pseudo
     CDS             complement(8702..9518)
                     /locus_tag="OO448_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9795..10058)
                     /gene="cedA"
                     /locus_tag="OO448_002550"
     CDS             complement(9795..10058)
                     /gene="cedA"
                     /locus_tag="OO448_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6337733.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10241..12502
                     /gene="katE"
                     /locus_tag="OO448_002551"
     CDS             10241..12502
                     /gene="katE"
                     /locus_tag="OO448_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6337734.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12760..13518)
                     /gene="chbG"
                     /locus_tag="OO448_002552"
     CDS             complement(12760..13518)
                     /gene="chbG"
                     /locus_tag="OO448_002552"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6337735.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13531..14883)
                     /gene="celF"
                     /locus_tag="OO448_002553"
                     /gene_synonym="chbF"
     CDS             complement(13531..14883)
                     /gene="celF"
                     /locus_tag="OO448_002553"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6337736.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14988..15830)
                     /gene="chbR"
                     /locus_tag="OO448_002554"
     CDS             complement(14988..15830)
                     /gene="chbR"
                     /locus_tag="OO448_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6337737.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15838..16188)
                     /gene="chbA"
                     /locus_tag="OO448_002555"
     CDS             complement(15838..16188)
                     /gene="chbA"
                     /locus_tag="OO448_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6337738.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16239..17597)
                     /gene="chbC"
                     /locus_tag="OO448_002556"
     CDS             complement(16239..17597)
                     /gene="chbC"
                     /locus_tag="OO448_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR6337739.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17682..18002)
                     /gene="chbB"
                     /locus_tag="OO448_002557"
     CDS             complement(17682..18002)
                     /gene="chbB"
                     /locus_tag="OO448_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6337740.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18301..18639)
                     /gene="osmE"
                     /locus_tag="OO448_002558"
     CDS             complement(18301..18639)
                     /gene="osmE"
                     /locus_tag="OO448_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6337741.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18841..19668
                     /gene="nadE"
                     /locus_tag="OO448_002559"
     CDS             18841..19668
                     /gene="nadE"
                     /locus_tag="OO448_002559"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6337742.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19898..20785
                     /gene="cho"
                     /locus_tag="OO448_002560"
     CDS             19898..20785
                     /gene="cho"
                     /locus_tag="OO448_002560"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6337743.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20745..21320)
                     /gene="ves"
                     /locus_tag="OO448_002561"
     CDS             complement(20745..21320)
                     /gene="ves"
                     /locus_tag="OO448_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6337744.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5738 a         5316 c         4940 g         5563 t
ORIGIN      
        1 gcccttgatt cattgagaaa tggaaaaggc aaagtcaacc tgattaaaca ctactcctcc
       61 gttgaatcca tacagcagca tgtcccctta gtccgggacg cggagttcag agcattactt
      121 cgccatcctc ctgcagggag tcgcgttatc gcgagtaagg attttggctt cgctttagat
      181 attttctttt gtcgaatgat ggcaaacaat gtcagtcata tgtccgcgat tttatatata
      241 gacaatcata ctttgtcagt aaggctacga ataaagcagt cagtgtatgg acaattaaat
      301 tatgttgtgt ctgtttacga cccgaacgat accaacgttg ccgtcagagg cacccacagg
      361 gcagcacggg gctttctctc gcttgataag ttcatcagtt cagctcccga tgctcagacc
      421 tgggctgata ggtatgttcg caactgtgca attgctattc ttcctctatt acctgaggga
      481 gttccagttg ctattttagc gggtatcacg acacgaatgc catttgcccc tatacatcca
      541 tcggcaatgt tgttaataat ggccacaggc cagactcaat agcttattac actattcaaa
      601 cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca gaatagcgtt
      661 ggtacacctg ctttatttct ggctatgatg aacggacaaa ctgacaacgt aaaaatattt
      721 atgcaagaaa ttcagtcact ggtatacaat catatcattc atgaagataa tctggttaaa
      781 ttactgcaaa ctaaaagtgc taacgaaaca cctggactct atatctccat gttgtatgga
      841 ttcgatgaaa taatcgatat ctttctgaat gcattaacca ctccaatagc acaagaactt
      901 ttaaacaaaa aaaatggtga tgagtatttt agccatgaaa atacatgatg gtgagccagg
      961 attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc tctctaaaat
     1021 taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt tattaaaagg
     1081 agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta atgaagacgt
     1141 cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt ttagtcaaca
     1201 tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg ttcatatagc
     1261 cattcatcat aagcattata aaactgtaga aacatattat gctgctataa atgtcatcag
     1321 ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag ctatttacga
     1381 tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga ccatgtgcac
     1441 aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctatccct tttgttacac
     1501 tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac gctttgataa
     1561 aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt tgttgctacg
     1621 ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga ccgcataaca
     1681 cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg acgatcggta
     1741 tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc caccttcagt
     1801 ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt tgtatcttcc
     1861 gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg ccatgcataa
     1921 gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata tttgtcgtaa
     1981 cgcacacctg ggccgaattc aaagcggaaa ctgtgtaccg ggccattcaa aaactggcga
     2041 ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc tgtcagccag
     2101 cttgcctgac caaataaata gtcataatca gttaaattaa aacggctacg cccgcccgcc
     2161 gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt cccccacagc
     2221 gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag tgaggagctt
     2281 ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa aggtttttta
     2341 gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc attcagtgac
     2401 gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag cttcatttat
     2461 caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc ttaaggatca
     2521 aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac ttagcaatgt
     2581 attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt tatatatatt
     2641 tatctgcaaa attttaaata aagctccaat aaatcatatt gttaatttct tcactttccg
     2701 ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg tatctatacg
     2761 ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta tcccgaagga
     2821 aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa cgtcgcccgc
     2881 gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg cgcgaccggc
     2941 gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt agaagccaaa
     3001 gactggaccc ggcagaattt acatgtacat gtggaagcaa gcggtgagca gtatcgtttt
     3061 gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga gcaagttctg
     3121 gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg tgtgaagctg
     3181 gaaaaattaa cccaactgat ttccgcttcg caaaaacaag ggatccgctg catcgtcgac
     3241 agttctggcg aagcgttaag tgcagcactg gcaattggta acatcgagtt ggttaagcct
     3301 aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccgga cgatgtccgc
     3361 aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt ttcccttggt
     3421 ccacaaggag cgctgggtgt tgatagtgaa aactgcattc aggtggtgcc accaccggtg
     3481 aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac actgaaactg
     3541 gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg gagtgcagcc
     3601 acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat ttacgcttac
     3661 ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa cctgtcggca
     3721 acgcgtttct ccgactttgc tcaaaagtca tgtgataaca aaggggtgaa ctatggccag
     3781 tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat tgactgaaat
     3841 taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga cggatgatga
     3901 gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc aaagtgaaga
     3961 agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag atcctgaaat
     4021 caccatcacg acttgggagg aatggaatag caacagaaaa taaatggttt ttgggcaata
     4081 atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc taaccttatg
     4141 atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc ttttgagcga
     4201 gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag aagtccatgc
     4261 cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg aaagggaact
     4321 gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta gtaaaaccgt
     4381 caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc tggtgatgga
     4441 ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc agcaaattgc
     4501 gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata actcgctctc
     4561 cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg ctgaacaacg
     4621 gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca atatcgacgc
     4681 catcgtcgag catattcagc agcgtctggc ctcgcatcaa ctgcagcctt ctctgttgca
     4741 cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca ttttcgaccc
     4801 tgcctgctac tggggtgacc gagagtgcga cctggcgatg ttaccgctgc atactgaaca
     4861 gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg atttccttga
     4921 acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat ttggcggtca
     4981 gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat atgggttgag
     5041 gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga aaagaacaca
     5101 taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg caggaaaata
     5161 gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc ctcttcccct
     5221 ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg cgcctggcga
     5281 ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat gaaaatcacc
     5341 cagaacataa cgttcacgcc accattaaaa ttaggggtcg gggagttata ccagaacaga
     5401 ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagtaaa gtcgagcatg
     5461 accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag caaagaaatc
     5521 aatgtggaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt caaaacagca
     5581 atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt cgtttcactt
     5641 agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat aggcgcttaa
     5701 tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg taatgtcaac
     5761 cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg actcagaacc
     5821 tttacgggat cgagccgaac tggatgtgat ggcaagcctg ggggtggata tctcccgtcg
     5881 taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc tttggtacgc
     5941 ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta ttgcccgtgc
     6001 catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag ctgttgcgtt
     6061 atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac atatgctgga
     6121 aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct cggctgaaaa
     6181 actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa aactgggcgt
     6241 tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg cttctaaagc
     6301 agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc cacgttttgt
     6361 attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc ttctcggtta
     6421 atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc attcaactga
     6481 cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt gcactgtata
     6541 aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg cggaaggtca
     6601 ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga cgcccgtgct
     6661 ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt tgttaccgga
     6721 catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa aaccgatcgc
     6781 ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg cgctgctggc
     6841 aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc tacaaggaat
     6901 ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg gtgttcccct
     6961 gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa agggcaacca
     7021 actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc cccattcatt
     7081 acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc agatccagtt
     7141 tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc tttctggaat
     7201 aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg cgccaacact
     7261 atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga actttccatt
     7321 aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga cccgccataa
     7381 acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg tttttggcct
     7441 tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg tacagtggtt
     7501 taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc cgttagtctt
     7561 cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag gcaaaatcag
     7621 ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg tcggtgtgct
     7681 ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg cagaaactgc
     7741 acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatctga gcgttccgca
     7801 gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag ccaatccgac
     7861 gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat tgaaactgct
     7921 gaaggatgat gcgccgaaag gtgaacgcgt cttagccgct atcgataccc tacaaagctg
     7981 ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc tggctctaat
     8041 gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa gtttcgttgt
     8101 cgcgtcctac ctcggtctgc tgattatgtt tgcagtgcat ggcattctgc tgggcattaa
     8161 tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt ttgcctttac
     8221 cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc gtcgtctggg
     8281 cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg gtcagaacgg
     8341 ctgcgccggt ttgtatccgg caatgctggc ggtgatggtt gcgcctacgg ttggcattaa
     8401 cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta gttccgcagg
     8461 cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc ctgcgatggg
     8521 cctgccagta accctggtgg cgctgttaat ctccgttgaa ccgcttatcg acatgggccg
     8581 tacggcgcta aacgttagtg gctcgatgac agctggcacg ctgaccagcc agtggctgaa
     8641 gcaaaccgat aaagccattc tggatagcga agacgacgcc gaactggcac accgttaatt
     8701 cttatgctgg caacggtccg ttttgtatag gggccgttgc tttacttttt aaatgttcca
     8761 tcgcggcgat gcatactgac tcatatttaa acagatcgtc agcgaaaaaa atgtccaact
     8821 tatcaataca ttcctggctc aatgaatgtg cctgtagtga gaagcttaaa acctcatacc
     8881 cgagacgttt tctaagtgaa ctatctggaa tactcataat atcttcaagc acaccacttc
     8941 ccattcccgg aagatttagt ggattaaaaa gaagattgat attaagcatt gatattatcg
     9001 aaggaaactt taggagactc tcattttttt tgaatcgcag caaaaatgct cccggcagga
     9061 tgtatataat ttttttcata gtatatagtc cttaaataat gaataaccaa cctatttttt
     9121 atattgatca aattcgtcat tttttcgggt cggaattcac aggtaaaatc catcccaaca
     9181 aaattctcat taatatctct tatccgaaac tcactgccat aaagattatt aattatcact
     9241 ctatatttaa cattgtcctg cagatacgta tcaaaactaa gtgctggagc tcctggcttt
     9301 gaggcatcat agttaatgtt agacatatag gcaatcatac gattgaagtt cgactctgct
     9361 atttcatcgc agtcacattg acccgttgat ataaagaaag tgagtgccgc catggtagac
     9421 tcaagccttt ctttaaaaga gtcgaaagcc aggcttttaa tatttaaatc accataatta
     9481 ctctgtatta agtttgtaga aaacatctcc cgccccatat tgttaacaaa attattatct
     9541 catttaaatc taagtcactt acaatataag tttaagagcg acgccacagg atgaactatc
     9601 aaaaatagct catcatgatt agcaaaactt aaccatttta aaataaataa acaattaaag
     9661 aaaaaagatc acttatttat agcaatagct cgtcaaaggc agctttttgt tacaggtggt
     9721 ttgaatgaat gtagcaacga aatgcagaat ttcaggtcat gtaactcccg gcaaaaccgg
     9781 gaagtatgta atccttactc agtcacttcc ccttcctggc ggatctgatt tgcccaacgt
     9841 tgggcagatt caggcacagt aaacgccggt gagcgcagaa atgactctcc catcagtaca
     9901 aacgcaacat atttgccacg cagcatccag acatcacgaa acgaatccat ctttatcgca
     9961 tgttctggcg gcgcgggttc cgtgcgtggg acatagctaa taatctggcg gttttgctgg
    10021 cggagcggtt tcttcattac tggcttcact aaacgcatat taaaaattag aaaaactgta
    10081 gtttagccga tttagcccct gtacgtcccg ctttgcgtgt atttcataac accgtttcca
    10141 gaatagtctc cgaagcggga tctggctggt ggtctatagt tagagagttt tttgaccaaa
    10201 acagcggccc tttcagtaat aaattaagga gacgagttca atgtcgcaac ataacgaaaa
    10261 gaacctacat cagcaccagt caccactaca cgattccagc gaagcgaaac cggggatgga
    10321 ctcactggca cctgaggacg gatctcatcg tccagcggct gaaccaacac cgccaggtgc
    10381 acaacctacc gcccctggga gcctgaaagc ccctgataca cgtaacgaaa aacttaattc
    10441 tctggaagac gtacgcaaag gcagtgaaaa ttatgcgctg accactaatc agggcgtgcg
    10501 catcgccgat gatcaaaact cactgcgtgc cggtaaccgt ggtccaacgc tgctggaaga
    10561 ttttattctg cgcgagaaaa tcacccactt tgaccatgag cgcatcccgg aacgtattgt
    10621 tcatgcacgc ggatcagcag ctcacggtta tttccagcca tataaaagct taagcgatat
    10681 caccaaagcg gatttcctct cagatccgaa caaaatcacc ccagtatttg ttaggttctc
    10741 taccgttcag ggtggcgctg gctctgcaga taccgtgcgt gatatccgtg gttttgccac
    10801 caagttctat actgaagagg gtatttttga cctcgttggc aataacacgc caatcttctt
    10861 tatccaggat gcgcataaat tccccgattt tgttcatgcg gtaaaaccag aaccgcactg
    10921 ggcaattcca caagggcaaa gtgcccacga tactttctgg gattatgttt ctctgcaacc
    10981 tgaaactctg cacaacgtga tgtgggcgat gtcggatcgc ggcatccccc gcagttaccg
    11041 caccatggaa ggcttcggta ttcacacctt ccgcctgatt aatgccgaag ggaaggcaac
    11101 gtttgtacgt ttccactgga aaccactggc aggtaaagcc tcactcgttt gggatgaagc
    11161 acaaaaactc accggacgtg acccggactt ccaccgccgc gagttgtggg aagccattga
    11221 agcaggcgat tttccggaat acgaactggg cttccagttg attcctgaag aagatgaatt
    11281 caagttcgac ttcgatcttc tcgatccaac caaactgatc ccggaagaac tggtgcccgt
    11341 tcagcgtgtc ggcaaaatgg tgctcaatcg caatccggat aacttctttg ccgaaaacga
    11401 acaagcagca ttccatccag gtcatattgt tcctggtctg gacttcacca acgatccgct
    11461 gttgcaggga cgtttgttct cctataccga tacacaaatc agtcgtcttg gtgggccgaa
    11521 tttccatgag attccgatta accgtccgac ctgcccttac cacaacttcc agcgtgatgg
    11581 catgcatcgt atggggatcg acactaaccc ggcgaattac gaaccgaact cgatcaacga
    11641 taactggccg cgcgaaacac cgccgggacc gaaacgcggc ggttttgaat catatcagga
    11701 acgcgtggaa ggaaataaag ttcgtgagcg cagtccatcg tttggcgaat attattccca
    11761 tccgcgtctg ttctggctaa gtcagacgcc attcgagcag cgccatattg tcgatggttt
    11821 cagttttgag ttaagcaaag tcgttcgtcc gtatattcgt gagcgcgttg ttgaccagct
    11881 ggcgcatatt gatctcactc tggcccaggc ggtggcgaaa aatctcggta tcgaactgac
    11941 tgacgaccag ctgaatatca ccccacctcc ggacgtcaac ggtctgaaaa aggatccatc
    12001 cttaagtttg tacgccattc ctgacggtga tgtgaaaggt cgcgtggtag cgattttact
    12061 taatgatgaa gtgagatcgg cagaccttct ggccattctc aaggccctga aggccaaagg
    12121 cgttcatgcc aaactgctct actcccgaat gggtgaagtg actgcggatg acggtacggt
    12181 gttgcctata gccgctacct ttgccggtgc accttcgctg acggtcgatg cggtcattgt
    12241 cccttgcggc aatatcgcgg atatcgctga caacggcgat gccaactact acctgatgga
    12301 agcctacaaa caccttaaac cgattgcgct ggcgggagac gcgcgcaagt ttaaagcaac
    12361 aatcaaggtc gctgaccagg gtgaagaagg gattgtggaa gctgacagcg ctgacggtag
    12421 ttttatggat gaactgctaa cgctgatggc agcacaccgc gtgtggtcac gcattcctaa
    12481 gattgacaaa attcctgcct gatgggaggc ggcgcaattg cgccgcctca aatgatttac
    12541 atagtgcgct ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaaaactgt
    12601 gcaaattcaa tatattgcag gaaacacgta ggcctgataa gcgaagcgca tcaggcagtt
    12661 ttgcgtttgt cagcagtctc aagcggcgca gttacgccgc ctttgtagga attaatcgcc
    12721 ggatgcaagg ttcacgccgc atctggcaaa catcctcact tacacatccc gataactccc
    12781 caaccgataa ccacgctgag cgatagcacc tttcaacgac gctgatgtca acacatccag
    12841 ctccgttaag cgtgggaaac agtaagcact ctgacggata gtattatcga taaacgccgg
    12901 atgacacatc acctccagcg aacgatcacc ccgatggctt gcgtcatcca gcacttgcag
    12961 gaacagtgcc tcgctaatct cttcaccata aaacgcgcta ctgaatccct gagaactgcg
    13021 cagattagcc ggtaaatcac cagagttaga tagcggctga cggtcgatgc gcaacgcaat
    13081 cccctcttca gccgcaaacc tggcaacaat cgggaaaatc tgcgggaaca tatgcacatg
    13141 atgatggcta tcaagatgcg taggtttgcg tccaaatagc tcaatgaaac gcaaatactg
    13201 actggcaagc tcctgagtaa tttcttccag cggtaaagca tcttcttctg ccaactgcca
    13261 gatccatttt cccagcacac catcgcgggt taaccccggc atagctgtca gtggcttgcc
    13321 catagtaagg acaaagtaca tccctatggc cagactcggt tcatcacgac tcaactgcac
    13381 cgcatggtca atagcctgcc cattcaccag cgccgtcgtc gatgtgacaa tcccattgcg
    13441 acaggcctcg ataatgccgt agttctgacc tttgcttaag ccaaaatcat cggcattaac
    13501 aatcagtaag cgttccataa tcagcctcgg ttaatgtgct tttttaagct ctgcgatgca
    13561 gtcggcaaaa tttggcagcc atttctcgtg cgccagaatc atctcgcgtg ccagcagctc
    13621 agcatcgcga tcggaatgca ccaacggact aaggtttagc gccagtaaca catcgttaaa
    13681 ttctccgcta agtgcggcgt tgctggcagc aatctcgaag cctttaatgg tgtgaatcag
    13741 ccccatcact ttatcatcga aatgcgtaat gcgcggatgt ggcgtcgcgc catcgcgccc
    13801 cagcttacag gtcatttcta ccgcccagtc tgccggaata ttatcaatat gcccatgatg
    13861 cgggatatta acgtaatgtt cagcttgctt gtcgttgtag atagcgttga tcacttcgca
    13921 cgctgcatca gagtaataag ccccaccgcg ctgttccagt tctttcggct taactttcaa
    13981 ctccgggttt ttatacagct caaaaagttg tttctcgact ttctgtacta cctgcgctcg
    14041 tgcgccgcct ttgtagtatt cgcccatttc aatagccagc atctcttttt gcttgaagta
    14101 ataaagcaaa taagaacacg gcaacagatt cagagaacga attaagcctt cgctaaatgg
    14161 cagatcgaaa atatttttaa cgccagatgc ttttaactgc ccggatgcca caccatcaag
    14221 caattcggca aagcgcgact tgccatttac cagcacatcc ttaatgaaca ccatatggtt
    14281 gaggccgaac aaatcgatag ataaatcatc gctgtctttc agcatcagaa catcgtgaat
    14341 aaacatcttc atgccgatcg gaatattaca cacgccgata aagcgtttaa atccggtatg
    14401 acgataaacg gcttcagtaa ccattcccgc cgggttagtg aagttaatca cccatgcatt
    14461 cggacaaagt tcttcgacat cttttacgat gtcaaaaatc accggaatgg tacgcagacc
    14521 tttaaacaga ccgcccgcgc cgttggtttc ctggccaaga taaccatgac ttaatggaat
    14581 acgttcatcc agttcgcgcg ccggtaattg gcctacgcgc agttgggtag taacgaaatc
    14641 agcatctttc aatgcttcgc ggcgatccag cgttttataa agcttcatcg ggacgccagc
    14701 gttatcaatc atccgttggc agagttcaaa aatgatatcc agtttctctt taccatcttc
    14761 gacatccacc agccataatt cgctgaccgg caattcgtga taacgcttaa taaatccttc
    14821 cagtaactcc ggggtatagc tgctcccgcc accaatagtg acgactttta atttctggct
    14881 cataatttct cccttcagta cagaatactg atatctggca tatctgcccc cccggacata
    14941 aataatccag caacaggaca gatatgtgaa ttgtcaggta taacgactta ctgattaaat
    15001 tcagtcaatt tcttacgata gctcttaggc gtaaaggacg ttaatttctt aaacgtttta
    15061 ataaacaaac tcgggctact ataaccggcc tcaaacgcaa tatccgtcac ggaatagttg
    15121 gtcatttcca gttgtttttt ggcaaaatta atacggatct cattaataat ctgcattggt
    15181 gttttgccat aatatcgttg agtcgctcgc gtcaaatatt cctgtgattt ggctgacaac
    15241 gtcaccatat tctccagcgc cgattcacta aactgctctt tatcatgcat cttttctacc
    15301 gtacttttca gccactgcgg tacatcatca atcacctgtt cttcgcggta atgacgtaaa
    15361 cggttaatga cataaaaagt aaccatctca acaaactctt ccagccctgt ttcgcggaaa
    15421 ttcaatgaag aaatcactgt ttccacatag gtgagaaaag cgttattggt ccggtatacc
    15481 tgcgaagcga caaagcaata aggcaacaac ggcaggtaat gctgctcaaa aaagcgttta
    15541 ctgatcccaa cgttcaatat acgcgtggca ccaaactcat aaaaactttg atggtgcgaa
    15601 cctaacggaa taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc gttaatctcc
    15661 tggaaataac gcccggttaa taccagagta aattcataat agtcgtgctg atgcagcccg
    15721 ctgatactct cagttttgtt atagataaac acatggaagt ttttgccatt aaacaactgc
    15781 tgttctcggg cagtggcaat ttccggcgcg ttaatcactg gctgcatcat cgactcctta
    15841 tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg caagcatgga
    15901 cgtcattaaa tgatcctgag cgtggaccag caccagactc actttcatct taccttcgcc
    15961 cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg ccatccgtga
    16021 ctgatccatc atggcttttg ctgcagcaaa atcgccctgc ttcgcctgtt tcagtgccgc
    16081 atacgccagg ctgcgcgctt gtccggagtt gatgataagc cccatcacca cttcttccag
    16141 ctcttcagct tccgtttgcg tatcgggaat gttatcgaga tccatcatac atcgttcctc
    16201 tctattctta ccggcacgat tacccgtacc ggcatcgatt aaaatttcag ggcgttagcg
    16261 atatcttctt cgcgctcttc tttatcaatc gcgttttgtg ctttgttagc caccacaaca
    16321 aagggcagat aaattaacgt tgcgatacca aggttgaaga gtgcaaccag caatgcggcg
    16381 acgctaccgt tggtgttaaa gaaggctccc agaccggttg gcatggtcca cggtgcaata
    16441 ttggtcaccg gaggaatgat gcccatgtag tacgctgcga gggtgattgc cgccagaatc
    16501 ggttgtacca gtacaaacgg gataaacatc accgggttca tgataattgg cagaccaaac
    16561 agaatcggtt cgttaatctg gaagatgccg gacggcagcg ccagttttgc cacctgacga
    16621 taatcagcac ggcgagaggc gataaagata gccaggatca ggcctaaagt cgcaccactg
    16681 cccccaagga aaataaagga gtccagcatc ggcttggccc agatatggaa ggtcttaccg
    16741 gctgccagcg ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc
    16801 cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag
    16861 aaccagagca gtggaacaaa gatcacatag gcccagccca ccacgctacc caacgatgcc
    16921 agtggggttg agatggtatc cataatgatc tgatggaagt tggtgcccca ggtattcaac
    16981 gcccaggcaa taatccccat cacggaaaga ataataaagc cgggaattaa tgccgagaag
    17041 gaacgcgata ctgaagcagg tacgctatcg ggcaatttaa tgacccaatt gcggcggaca
    17101 ataaaggtaa acatttctgc caccaccagg ccaataataa tcccggagat gatattcgct
    17161 ccacctaacc agtttgcgcc aaccacatag gcctcaccga cactatatgg ggtgacggtc
    17221 ataaatgctg caacggataa caacccagcc gccagcgcat cgactttacg ctcttctgcc
    17281 agcgccatgc caataaagaa cggtgccatt aaagacatta tccctaatgt tccgttatat
    17341 acgttgccgc caataccttt caggccatta agtgtttcaa tggttgaggc gtcgaggcga
    17401 atacctaagg aataaaaaaa cgccccctcc ccaaagctta gaaaaacgtt gttaattaat
    17461 acaaacatgg cccccgcaag ggttaacggc attaagcgaa taaagccatt tttgattgca
    17521 ttaacgtgtg gctgctttcc tattttaact gcaaaaggga ggagtacctt ttcaagcgat
    17581 gcaataacat tactcataga aaaataccct taaaaaccgc aatttaaata ttgcggtatt
    17641 gatttatgaa ataactcttt gacgggaaaa tttaaaataa attaatttgc tgcggctttt
    17701 ttaatcgctg caaccgcagc cttaagcacg cctaaaccat cgactttgcc ataaagcagc
    17761 gagtcaatta cttcaaccgg tttgttgggt aacaaacgct ggatttcggg caacatataa
    17821 gcaatctgcg gccctaataa cacgacatcg gcattctgac ctttttcacc agccagtgtt
    17881 tccggaaatg cttcaataat gaccggaact tcatattttt ctgcctgtgc gcgcattttt
    17941 gataccagta aagaggtaga catgcccgca gaacaaaaca gataaatgtg tttcttttcc
    18001 ataaaactgc cctcatcgac gattatctgt cagccagaca ctccgcaagc cttaacctgc
    18061 ttccatgctc tgggtaactt gcgaaaccaa catgactttt tttgtggctg aaaggagtat
    18121 acggtataga cctgagcaat agtatttctt tgaccttata aattgtttct ccttgacctg
    18181 tggttatgac cctcttcaca tttcgggcaa ataattcgcg aagataatta agaactcagg
    18241 ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact caagctttca gacgaattga
    18301 ttacttcgca gcctgtggat cagtgtcgta ttcagcacag gtctgataac cggagttgat
    18361 gacatgtccg gtatcatcta acgcgacaaa gtaggtttct gctttaccat cacgttgacc
    18421 caggatgtag gtctggcaag taccacgagc atggatcatg ctcacttcag acgaaggttt
    18481 acccgcaatt tgtgcaacct gcgcccggct catgcctttt ttgacgtctt tcaccacagg
    18541 ctgtacaaac tggtctttag tacgatcata agccgtacaa cccgccagca tggttaatac
    18601 cgccgctgca ctcagaattc ctgccatatt cttgttcata ttccgtcctc ttgtttatca
    18661 gcgtgttaga taagcctgga atacattggg cgctttttca agcccgcgaa cgaaacggct
    18721 ccgctttcag aggattcctg tatgacgttt taaccaccat tcagcccgct gtcgcttgtc
    18781 gtttcagtgg caacgggtta gctttaagga agttttgtct tttctgtctg gaggggttca
    18841 atgacattgc aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa
    18901 gaggaaattc gtcgtagtat cgattttctg aaaagctacc tgcaaactta tccgttcatt
    18961 aaatcactgg tgctcgggat cagcggcggt caggactcca cgcttgccgg aaagctgtgc
    19021 cagatggcga ttaatgagct gcgcctggaa accggcaacg aatcactgca atttattgcc
    19081 gtacgcctgc cctatggtgt tcaggccgac gaacaagatt gccaggatgc cattgccttt
    19141 attcaaccgg atcgcgtatt aaccgttaat atcaagggcg cggtattggc cagcgaacag
    19201 gcattgcggg aagcaggcat tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt
    19261 gagcggatga aagcacaata tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc
    19321 gatcatgcag cagaagccat taccggattc ttcactaaat atggtgacgg cggtacagac
    19381 attaatccgc tgtatcgtct caacaaacgt cagggtaaac agttactggc ggcattaggt
    19441 tgcccggaac acctttataa gaaagcgcca acggccgatc tggaagatga tcgcccttct
    19501 ctgccagatg aagtggcact cggcgtgacc tatgacaata tcgacgacta tctggaaggg
    19561 aaaaacgtac ctgaacaggt cgccagaaca atagagaact ggtatctgaa aaccgaacat
    19621 aaacgccgtc cgccaattac cgttttcgat gatttctgga aaaagtaata atttgcacat
    19681 aatggtttgt gcgaaaaaga gtaatttgtt cacgccggat gcggcgtgaa cgccttattc
    19741 gacctacaaa actatgcaaa ttcaatatat tgcaggagtc gaataggcct gacaggcgta
    19801 gcacgtcaga cggtgtaacc tttgtcatcg acccgcctct tttttaatcg ctccccgcct
    19861 gttacactgg atagataacc agcattcgga gtcaacagtg gtacggcgtt taacttctcc
    19921 gcggctcgaa tttgaagctg cggcaattta tgaatatccc gaacatttac gttcattcct
    19981 taatgactta cccacccgac caggggtgta tctgtttcat ggcgaaagtg acaccatgcc
    20041 gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg ctttctcatt tacgcacccc
    20101 ggatgaagcc gccatgctac ggcaatcccg gcgaattagc tggatctgta ccgcaggcga
    20161 aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa caacagccgc tgtttaataa
    20221 acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt
    20281 ggtgtatgcc aaagaggtgg atttttcacg agcccccaac ctgtttggcc tgtttgccaa
    20341 taggcgcgca gctttgcaag cattgcagac catcgctgat gaacaaaaac tttgttatgg
    20401 cctgctggga ctggaaccgt taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg
    20461 ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa catgcgctac gcttgcgcca
    20521 gtctctggag cgtttgcggg tggtgtgttg gccttggcaa ggtgcggtgg cactgaaaga
    20581 acagcacccg gaaatgactc aatatcatat tattcaaaac tggctgtggc tgggggcggt
    20641 taattcgctg gaagaagcga caacgttaat tcggacaccc gccgggtttg atcacgacgg
    20701 ttataaaatt ctttgtaagc cgctgctttc cggtaactat gaaattactg aacttgatcc
    20761 ggcgaatgac cagcgagcca gttgatttca ctgaacaaca gtttaccttg cggttgtaaa
    20821 agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt gagcaattta
    20881 tcgcccagtt gccacgcccc gttaatcaca aacaccaccc cgccgcgtga gccaaaggta
    20941 gtaaacgtac gttcggcaat tctgactttc gccttacaga catccaggcg tgtcatgatg
    21001 ttgaaatcca tcgacatctg cccttccgtc agtttcgctt ttaccacctg gtccgctgca
    21061 aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc ggtctgcgct ttcaaggagc
    21121 atctcgccgc cttccagcaa cgtcactatc ctttccatgc cgggaaacaa agaaaactcc
    21181 ccattcgccg cgatggatgc aatgctggca cgccagtaaa aatcacgttt tgccggtggg
    21241 aaagtgcaaa tttcgcgcgt ttcaccggca gcatttcgcc acagattcac cgacatttta
    21301 cgcatatcaa agtattccat actcgctccc ggcccgttct tttcattgtt atcgttcagg
    21361 taaccgtgga tggttatcgg caactcgggt gatttacgtt agtggtgatc aggctttttt
    21421 tacaactgtt tgagaagaga aaagaaaacc gccgatcctg tccaccgcat tactgcaagg
    21481 tagtggacaa gaccggcggt cttaagtttt ttggctgaaa gattattcag cagttgcagg
    21541 cattttacct tttgccg
//
LOCUS       DAJTVJ010000071        21276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000071 DAJTVJ010000000
VERSION     DAJTVJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.071"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(152..697)
                     /gene="hemG"
                     /locus_tag="OO448_002562"
     CDS             complement(152..697)
                     /gene="hemG"
                     /locus_tag="OO448_002562"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6337745.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(709..2160)
                     /gene="trkH"
                     /locus_tag="OO448_002563"
     CDS             complement(709..2160)
                     /gene="trkH"
                     /locus_tag="OO448_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6337746.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2199..2813)
                     /locus_tag="OO448_002564"
     CDS             complement(2199..2813)
                     /locus_tag="OO448_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6337747.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2813..4144)
                     /gene="pepQ"
                     /locus_tag="OO448_002565"
     CDS             complement(2813..4144)
                     /gene="pepQ"
                     /locus_tag="OO448_002565"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6337748.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVELLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4334..6523
                     /gene="fadB"
                     /locus_tag="OO448_002566"
     CDS             4334..6523
                     /gene="fadB"
                     /locus_tag="OO448_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6337749.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6533..7696
                     /gene="fadA"
                     /locus_tag="OO448_002567"
     CDS             6533..7696
                     /gene="fadA"
                     /locus_tag="OO448_002567"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6337750.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7886..8587)
                     /gene="fre"
                     /locus_tag="OO448_002568"
     CDS             complement(7886..8587)
                     /gene="fre"
                     /locus_tag="OO448_002568"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6337751.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8634..10127)
                     /gene="ubiD"
                     /locus_tag="OO448_002569"
     CDS             complement(8634..10127)
                     /gene="ubiD"
                     /locus_tag="OO448_002569"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6337752.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10294..10782
                     /gene="rfaH"
                     /locus_tag="OO448_002570"
     CDS             10294..10782
                     /gene="rfaH"
                     /locus_tag="OO448_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6337753.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10779..11561)
                     /gene="tatD"
                     /locus_tag="OO448_002571"
     CDS             complement(10779..11561)
                     /gene="tatD"
                     /locus_tag="OO448_002571"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6337754.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11603..12379)
                     /gene="tatC"
                     /locus_tag="OO448_002572"
     CDS             complement(11603..12379)
                     /gene="tatC"
                     /locus_tag="OO448_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6337755.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12382..12897)
                     /gene="tatB"
                     /locus_tag="OO448_002573"
     CDS             complement(12382..12897)
                     /gene="tatB"
                     /locus_tag="OO448_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6337756.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12901..13170)
                     /gene="tatA"
                     /locus_tag="OO448_002574"
     CDS             complement(12901..13170)
                     /gene="tatA"
                     /locus_tag="OO448_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6337757.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13249..14889)
                     /gene="ubiB"
                     /locus_tag="OO448_002575"
     CDS             complement(13249..14889)
                     /gene="ubiB"
                     /locus_tag="OO448_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6337758.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14886..15491)
                     /gene="ubiJ"
                     /locus_tag="OO448_002576"
     CDS             complement(14886..15491)
                     /gene="ubiJ"
                     /locus_tag="OO448_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6337759.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15505..16260)
                     /gene="ubiE"
                     /locus_tag="OO448_002577"
     CDS             complement(15505..16260)
                     /gene="ubiE"
                     /locus_tag="OO448_002577"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6337760.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16355..17782)
                     /gene="rmuC"
                     /locus_tag="OO448_002578"
     CDS             complement(16355..17782)
                     /gene="rmuC"
                     /locus_tag="OO448_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6337761.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17923..18684)
                     /gene="udp"
                     /locus_tag="OO448_002579"
     CDS             complement(17923..18684)
                     /gene="udp"
                     /locus_tag="OO448_002579"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6337762.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18946..19758
                     /locus_tag="OO448_002580"
     CDS             18946..19758
                     /locus_tag="OO448_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6337763.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20152..21213
                     /gene="hcp"
                     /locus_tag="OO448_002581"
                     /pseudo
     CDS             20152..21213
                     /gene="hcp"
                     /locus_tag="OO448_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5227 a         5699 c         5338 g         5012 t
ORIGIN      
        1 ctctgtcgga attctcagga gaaccccgct gacccggcgg cgtgtttgcc gttgttccgt
       61 gtcagtggtg gcgcattata gggagttatt ccggcctgac aagaggaaat ttaaaataat
      121 tttctgaccg cgcatttttt attctttatg cttatttcag cgtcggtttg ttggttaact
      181 gggcgatttc tcgggcgaaa ttcgccacct gctcccaatc ggtatagaca acttctttgc
      241 gcgtatccgt ttcaccgcct gacatcttca taattagctt gatcataaaa cgatcgtacc
      301 agcgataacg tgggtaacgc agcgccccgg caatgaccgc gcagcgatcg ggacgccatt
      361 gcgagttcat cagaaacttc cgcgcgtagc tgttggtctg tggagtacgc ttctccggtt
      421 tgcgcgccac caggtttacg gaataaaagg cgctcggcat cgaattcagt cgcgtcgcat
      481 gttttttgac aaattcctgg aacgcagagt ggtagtgacc atagcgaata gaagcaccaa
      541 tgaccacacg gtcataattt tcccattgtg gttcttcaat gcggtgcaca ttggcgacat
      601 ccgtctggat ccccagttct ttcagttccg aagccaggta ggaggcaatc tcgcgcgttt
      661 gtccgtccct tgttgagaaa agaattaatg ttttcacgta ttactccatc attcacgcca
      721 gaaagtcggg gtaaagagca ccagcaatgt aaagacctcg agacgaccaa acagcatgtt
      781 ggcaatcagg atccatttag ccaccgggtt catactggta aagttatcag caaccacgcc
      841 aagccctggc cccaggttat tcaatgtcgc aacaactgaa gcaaaggcag aaaagtcatc
      901 cacgcccgtg gcgataatcg ccagcatact gacaataaac accaatgcat aggcggagaa
      961 aaatccccaa acggcttcga ggatacgttc cggcagtgcg cgattcccca gcttaatgct
     1021 atagacggcg ttcggatgca ccagtcgttt cagctcacgg ttcccctgct taaacagcag
     1081 caggatgcgg atcactttca ggccaccgcc cgttgacccg gcgcaaccgc cgataaaagc
     1141 tgaacataaa agcagtaccg gcaaaaagag cggccagcgg gcaatgctgt cagtcgtaaa
     1201 cccggcggtt gtcgccatcg ataccacctg gaaaaacgcc tggttaattg tcatcagcgc
     1261 cgaactgtag acattatgaa accacagtac gagtgtacaa ataaccacca gcgtaaactg
     1321 tacgccgata aacatgcgaa attccggatc gcgccaataa accttcagac tacgcccact
     1381 taacagtgaa aagtgcagac cgtagttaca gccggagatc agcaggaaga tagcaatgat
     1441 agtgttaata gtcgggctgt cgaaataacc gatactggca tcatgtgtcg agaagccgcc
     1501 aatagcgata gtcgcaaagc tatggccgat ggcatcaaag gcatccattc cggcaaacca
     1561 caacgccagc gcacaggcga cggtcagcaa gacataaatc aaccacaggg ttttcgccgt
     1621 ttccgcaatt cgcgggcgca ttttgttatc tttcagcggg ccgggcattt ctgcgcgata
     1681 gagctgcatc ccacccacgc cgaggatagg cagtatcgca accgctaaca cgatgatccc
     1741 catcccgcca aaccattgca gcatctggcg ataaaagagg atggcatgag ggagcgaatc
     1801 cagccccacc agcgtagtgg ccccggtggt ggtcaggcca gagaaagatt caaaaaacgc
     1861 atcggtaatc gtgaggttcg ggctttccga gaagataaaa gggagcgcac cgacactacc
     1921 cagcacggtc cagaacagca ccactatcag aaacccctca cgggatttca gttcgccctt
     1981 ctctttacgg ttcggccacc acagcataga accaatggcg agggcgacaa aaaaggtctg
     2041 ggtaaaagcg cggcccgctc catcccggta gatgagtgct accagcccag ggataatcat
     2101 ggtccctgaa aataagatga ccagtagtcc aacgattcgg gtaatggcgc gaaaatgcat
     2161 ctctgccgct tccttagttc tgcaaaacga agtggggatt attcttcaat cgctaacaat
     2221 tgcaatgaac cacggctaaa atccgccagc tttgcggaaa attcagccac tttcgccgcc
     2281 ggaagcgcca cccgcagcag aacgaatgcc tgataatcac tgttgataat tttgccgtca
     2341 cactgcccca gcaacgcttc aatgccggtt aactgatgat attcacattg caaagtatat
     2401 tcggttaatg gcgtcttgcg ttgggtcgtt agctggcgca gcgcctgatt cacgccgccg
     2461 ccatacgctt tcactaaccc accggtgcca agcaatatgc cgccgtagta gcgcactacc
     2521 acagaggtaa tttccccgac gccgctgccc attagctggg cgagcatcgg tttacctgcc
     2581 gttcctgccg gctccccgtc gtcagagaaa cccagctgtt gagaatcatc cggcgcaccc
     2641 gccacccacg ccacgcaatg gtggcgggca tcggggtgtt ctgcccgcac cgattcaaca
     2701 aacgctttcg ccgcctcaac gccatcggta tgcgccaaca tcgttatgaa gcggcttttt
     2761 ttgatctctt caacgaccgt gaccggtgcc gcaggaatta accagctttc catcacgcca
     2821 gtttcagatc ccgggtcatg ttttccacgt tgttttcgtg gatcaccacg ttgtcttcga
     2881 tacgaatgcc gccgaacggt ttcagtgctt caattttctg ccagttgaag tgcttgctga
     2941 actgcccttc acgccacggt gccagtagcg attcaatgaa gtagataccc ggttcgatgg
     3001 ttaacaccat acccggctgg agaatacggg tgcagcgcag gtacggatat tttgccggtg
     3061 ccgcgaggtg tgtaccgcta tcatcctgca taaaaccggc tacgtcatgc acctgcaggc
     3121 ccagcggatg accgatacca tgcggcataa acggtccggt gagatcgttt tcgaccatcg
     3181 cttcttcact catatcggtg atgatttgat gtttacgcag cagtttggcg atgcgctgat
     3241 ggaactggat gtggtaatcc acatagctaa cgcctgcttt catggtggcg atcagcgcca
     3301 gttgttcatc atttacgtct ttcaccagct gtgcgtagtc gttgtcgctt tttgccgacc
     3361 aggtacgagt caggtcagcc gcatagccgt tatattcggc cccggcatcc agcaggaagc
     3421 tgcgcatctc ttccggtgcc tgatgatcca gtttggtgta atgcagcacc gaagcgtgtt
     3481 cgttaagcgc cacaatgttg ctgtaaggta cgtcggtatc acgatgaccg gtcgcggtca
     3541 gataggcgat gttgatatcg aactcgctca tgccagaacg gaacgcttct tctgccgcac
     3601 gatgaccgtt gaccgccatt ttctgcgctt cacgcataca ggccagttcg tactcggttt
     3661 tgaaggagcg gtagtaatgc aggtagtcga tcaccccttt cgggttgatg ttgctggcct
     3721 caatacccag ttgcagcgca cgttccggca ccggaccgat ataaccgata ttgccgcgcg
     3781 cagcgggcag caggctacca atgccatcgg ctttcggcag tgcgatcact tccacatctt
     3841 cagtccagaa ggaggtcggc agcagttcga cgttgtgcca gtaatcaacc ggcagataga
     3901 accacagttt cggcttgttc acgccatcca ccagcaacca gcagtttggc acctgagtta
     3961 ccggcaccca cgctttgaat tgcgggttca ctttaaacgg atagggatga tcgtcgagaa
     4021 aaacgttgaa cagctcgccg gagtgaataa gtaatgcatc cagcttgaag cgcgccagcg
     4081 catcgcgagt ccgttcttgt aaggtagcta tatgattttt atagagcgag gccagtgatt
     4141 ccatttttta cccttctgtt tttttgacct taagtctccg catcttagca catcgttcat
     4201 ccagagcgtg atttctgccg agcgtgatca gatcggcatt tctttaatct tttatttgca
     4261 tatttttaac acaaaataca cacttcgatg catctggtac gaccagatca ccttgcggat
     4321 tcaggagact gacatgcttt acaaaggcga caccctgtac cttgactggc tggaagatgg
     4381 cattgccgaa ctggtatttg atgccccagg ttcagttaat aaactcgaca ctgcgaccgt
     4441 cgccagcctc ggcgaggcca tcggcgtgct ggaacagcaa tcagatctaa aagggctgct
     4501 gctgcgttcg aacaaagcag cctttatcgt cggtgctgat atcaccgaat ttttgtccct
     4561 gttcctcgtt cctgaagaac agttaagtca gtggctgcac tttgccaata gcgtgtttaa
     4621 tcgcctggaa gatctgccgg tgccgaccat tgctgccgtc aacggctatg cgctgggtgg
     4681 tggctgcgaa tgcgtgctgg cgaccgatta tcgtctggcg acgccggatc tgcgcatcgg
     4741 tctgccggaa accaaactgg gcatcatgcc gggctttggc ggttctgtac gtatgccacg
     4801 tatgctgggc gctgacagtg cgctggaaat cattgccgcc ggtaaagatg tcggcgcgga
     4861 tcaggcgctg aaaatcggtc tggtggatgg ggtagtcaaa gcagaaaaac tggttgaagg
     4921 cgcaaaggcg gttttacgcc aggccattaa cggcgacctc gactggaaag cgaaacgcca
     4981 gccgaagctg gaaccgctaa aactgagcaa gattgaagcc accatgagct tcaccatcgc
     5041 taaaggaatg gtcgcgcaaa cggcggggaa acattatccg gcccccatca ccgcagtaaa
     5101 aaccattgaa gctgcggccc gttttggtcg tgaagaagcc ttaaatctgg aaaacaaaag
     5161 ttttgtcccg ctggcgcata ccaacgaagc tcgcgcactg gtcggcattt tccttaacga
     5221 tcaatatgta aaaggcaaag cgaagaaact caccaaagac gttgaaaccc cgaaacaggc
     5281 tgctgtgctg ggcgcaggca tcatgggtgg cggtatcgct taccagtccg cgtggaaagg
     5341 cgtgccggtt gtcatgaaag atatcaacga caagtcgtta accctcggca tgaccgaagc
     5401 cgcgaaactg ctgaacaagc agcttgagcg cggcaagatc gatggtctga aactggctgg
     5461 cgtgatctcc acgatccacc caacgctcga ctacgccgga tttgaccgcg tggatattgt
     5521 ggtagaagcg gttgttgaaa acccgaaagt gaaaaaagcc gtactggcag aaaccgaaca
     5581 aaaagtacgc caggataccg tgctggcgtc taacacttca accattccta tcagcgaact
     5641 ggccaacgcg ctggaacgcc cggaaaactt ctgcgggatg cacttcttta acccggtcca
     5701 ccgaatgccg ttggtagaaa ttattcgcgg cgagaaaagc tccgacgaaa ccatcgcgaa
     5761 agttgtcgcc tgggcgagca agatgggcaa gacgccgatt gtggttaacg actgccccgg
     5821 cttctttgtt aaccgcgtgc tgttcccgta tttcgccggt ttcagccagc tgctgcgcga
     5881 cggcgcggat ttccgcaaga tcgacaaagt gatggaaaaa cagtttggct ggccgatggg
     5941 cccggcatat ctgctggacg ttgtgggcat tgataccgcg catcacgctc aggctgtcat
     6001 ggcagcaggc ttcccgcagc ggatgcaaaa agattaccgc gatgccatcg acgcgctgtt
     6061 tgatgccaac cgctttggtc agaagaacgg cctcggtttc tggcgttata aagaagacag
     6121 caaaggtaag ccgaagaaag aagaagacgc cgccgttgaa gacctgctgg cagaagtgag
     6181 ccagccgaag cgcgatttca gcgaagaaga gattatcgcc cgcatgatga tcccgatggt
     6241 caacgaagtg gtgcgctgtc tggaagaggg cattatcgcc actccggcgg aagcggatat
     6301 ggcgctggtc tacggcctgg gcttccctcc gttccacggc ggcgcgttcc gctggctgga
     6361 caccctcggt agcgcaaaat acctcgatat ggcacagcaa tatcagcacc tcggcccgct
     6421 gtatgaagtg ccggaaggtc tgcgtaataa agcgcgtcat aacgaaccgt actatcctcc
     6481 ggttgagcca gcccgtccgg ttggcgacct gaaaacggct taaggagtca caatggaaca
     6541 ggttgtcatt gtcgatgcaa ttcgcacccc gatgggccgt tcgaagggcg gtgcttttcg
     6601 taacgtgcgt gcagaagatc tctccgctca tttaatgcgt agcctgctgg cgcgtaaccc
     6661 ggcgctggaa gcggcggccc tcgacgatat ttactggggt tgtgtgcagc agacgctgga
     6721 gcagggtttt aatatcgccc gtaacgcggc gctgctggca gaagtgccgc actctgtccc
     6781 ggcggttacc gtcaatcgct tgtgtggttc atccatgcag gcactgcatg acgcagcacg
     6841 aatgatcatg actggcgatg cgcaggcagg tctggttggc ggcgtggagc atatgggcca
     6901 tgtgccgatg agtcacggcg tcgattttca ccccggcctg agccgcaatg tcgccaaagc
     6961 ggcgggcatg atgggcttaa cggcagaaat gctggcgcgt atgcacggta tcagccgtga
     7021 aatgcaggat gcctttgccg cgcggtcaca cgcccgcgcc tgggccgcca cgcagtcggg
     7081 cgcatttaaa aatgaaatca tcccgaccgg tggtcacgat gccgacggcg tcctgaagca
     7141 gtttaattac gacgaagtga ttcgcccgga aaccaccgtg gaagccctcg ccacgctgcg
     7201 tccggcgttt gatccagtaa gcggtacggt aacggcgggt acatcttctg cactttctga
     7261 tggtgccgcg gccatgctgg tgatgagtga aagccgcgcc cgtgaattag gtcttaagcc
     7321 gcgcgcccgt gtgcgttcga tggcggtcgt tggttgtgat ccgtcgatta tgggttacgg
     7381 cccggttcct gcctcgaagc tggcgctgaa aaaagcgggg ctttctgcca gcgatatcgg
     7441 cgtgtttgag atgaacgaag cctttgccgc gcagatcctg ccgtgcatta aagatctcgg
     7501 gctaatggag cagattgacg agaagatcaa cctcaatggt ggtgcgatcg cgctgggtca
     7561 tccgctgggt tgttccggtg cgcgtatcag caccacgttg ctgaatctga tggaacacaa
     7621 agacgttcag tttggtctgg cgacgatgtg tatcggtctg ggtcagggta ttgcgacggt
     7681 gtttgagcgg gtttaaccac ttgccggatg cggcgtgaac gccttatccg gtctaccgat
     7741 cctgcaccaa tgtaggcctg ataagacgcg ccagcgtcgc atcaggcatc gtgcaccgat
     7801 tgccggatgc gacatagatc taaatgccac tgaataaagt tttagttgcc gttcttcccg
     7861 cctgtcaggg gcgggttttt atatctcaga taaatgcaaa cgcatcgcca aacagacgat
     7921 cttcccgcgc attacgctca ctgcaaaaca ggtcacgagc aattttcgcc atctcaaaac
     7981 gtccggcaat atagatatca tgctctgcca gcgtaccatg atcctgcaag accgccgtta
     8041 acacggtgcc agtacgccca cgccagcccg cttccggttg ttcaaccacc ggcaccactt
     8101 gcagaccagg atgcttcagc gaaagcgcct caagctcgca gagatcatac agatgttgct
     8161 cttcacgccc accccagtaa atggtgatat cgcgattggg attacgcgcc aacgccgtca
     8221 ataaaatcga gcgcgcatag gagaacccgg tgccgcccgc aatcaaaatc atcggacgct
     8281 cttcatcatc gcgcagccac gcttctccgt ggggaatatc gaccacgatt tgatgatctt
     8341 tgaggatgcg gtccatgact gctttcgcgt aaaggttgat ttcagaagcg ccaatatgca
     8401 gctcgataaa ccctttttca tccggcgtcg aagccattga aaacggacgt ttgtcgcgct
     8461 catccatcac taccatcaaa tactgaccag cacgaaaaga aaaggccgcg tctggtacga
     8521 ttcggacacg gtatacagta tccgtgatag cttctaccga ggtcacttta cagcttaagg
     8581 ttgtcatgcg ctttctctgt cggatcaaat aaatagggca aaactaacgc gcatcaggcg
     8641 cttttaccgt tgttaaaaat agccagttca tcccagatgg cgtcaatatg cgcgacgaca
     8701 tcaggatctt ttttgatggg acgtccccat tcacgctggg tttcccccgg ccatttattc
     8761 gtggcatcca gccccatttt tgaacccagc ccggagacag gcgaggcaaa atccagataa
     8821 tcaataggcg tattttctac cagaacagta tcccgcgccg ggtccatacg ggtggtaatc
     8881 gcccaaatca catcgttcca gtcgcgtgcg ttgacgtcat catcgcaaac gatcacaaat
     8941 ttagtgtaca taaactggcg taagaacgac cagacgccca tcatgacgcg cttcgcgtgt
     9001 ccggcgtact gttttttgat tgtcactacc gccagacgat aagaacagcc ttccggtggc
     9061 aggtaaaaat cgacaatttc cgggaactgt ttttgcagaa tcggcacaaa cacttcgttc
     9121 agtgcgacac ccagcaccgc gggttcatcc ggcggacgac cagtataggt ggagtggtaa
     9181 atcgcatctt cacgctgggt aatatgcgtc acggtaaata ccgggaaact atcgacttcg
     9241 ttatagtaac cggtgtggtc gccatacggc ccttccggcg cagtttcgcc ttgttcgata
     9301 tacccttcca gcacaatctc cgcactggcg ggcacttcaa ggtcattgga gatacacttc
     9361 accacttcgg tcttggtacc gcgcagcaat ccggcaaacg catactctga aagtgtatcc
     9421 ggaacggggg tgactgcacc gagaatcgtg gcgggatcgg cacccagcgc cacagaaacc
     9481 gggaaacgtt cgcccggatg cgccgcacac cactcctgat aatccagcgc gccgccgcga
     9541 tgcgacagcc agcgcataat cagtttgttt ttaccaatca gctgctggcg ataaatgccc
     9601 agattctgcc gctctttatg cgggccgcgc gttacggtca gcccccaggt aatcagcggc
     9661 gcggcatctt ccggccagca ggtcataatg ggaatgcgat tgagatcgac gtcatcgcca
     9721 gagacgattt tttgttggca gggcgcacca cgcagccgct ttgtcggcat gttcaatact
     9781 tgcttaaact gcggcagttt atcaaacagg tcgcggaaac cttttggcgg ctcgggctct
     9841 ttcagaaacg ccaataattt accaacttca cgcagcgccg aaacatcttc ctgccccatg
     9901 cccatcgcca cgcgctttgg cgtaccgaac aggttgcaca gcaccggcat tgagtagcct
     9961 ttagggtttt cgaacaacag cgcaggccca ccggcacgca aagtgcggtc agcaatttca
    10021 gtgatttcca gatgcggatc caccgggagc gtgatacgtt ttagctcacc ctgctgttca
    10081 agcagcgtca agaagtcgcg taaatcgtta tatttcatgg cgtccattgt agcctcttaa
    10141 tctacgccca ttatacggcg ttcatctttg cgatgctgta aatttgttaa attagcgtga
    10201 actctgacgg tataacgcaa accggggaat ataattaact tagcgtaaag cttttgctat
    10261 ccttgcgccc cgattaaacg gataagagtc attatgcaat cttggtattt actgtactgc
    10321 aagcgcgggc aacttcaacg tgcccaggaa caccttgaga gacaggctgt gaattgcctg
    10381 gcaccgatga tcaccctgga aaaaatcgtg cgtggaaaac gtactgcagt cagtgagcca
    10441 ttgtttccca actacctgtt tgtcgaattt gatccagaag tgattcatac cacgactatc
    10501 aacgcgaccc gcggtgtcag ccacttcgtg cgctttggcg cgtcgctagc gatagtccca
    10561 tcggcggtga ttcatcagct atcggtatat aaaccgaaag acattgtcga tccggcaacc
    10621 ccttatccgg gagataaggt gattattacc gaaggcgcgt tcgaaggctt tcaggccatt
    10681 ttcaccgaac ccgatggtga ggctcgctcc atgctattgc ttaatcttat taataaagag
    10741 attaagcaca gtgtgaagaa caccgagttc cgcaaactct aaaacgcaat cccaaaccgt
    10801 gttttgacat tagcatccgt ggtggcagcc agccatgcgg catcttctcc acgccagtgc
    10861 gcaatacgtt gcaaaatatg gggcagatgg gctggctcgt tgcgccggga tgatggcttt
    10921 ggcgtgagat cgcgagggag cagatacggc gcatcagttt cgatcagtaa tttttccgcc
    10981 ggaatcaacg gcaaaagttc ccgcagctcc agtccgcgtc gttcatcgta aacccaaccg
    11041 gtaatgccga tataaattcc atgcgccacg cacgcctgca tctcttcgcg tgtgccggta
    11101 aagcaatgaa gaaccgcacc aggcagttta tccagccacg gctccagcaa tgtcataaac
    11161 cgctcgtggg catcgcgaca gtgcataaat accggcatgt ttaaatctgc ggcaatgcgt
    11221 agctgggcaa caaaagcgcg ttcctgctct tccggcgtcg aaaagttgcg gttaaagtcg
    11281 agaccacatt caccaatcgc caccacttct ggctgcgcgg ccagctcaat aatcgcttct
    11341 tcagtcgcag cttgccactg gctgctgtcg tgaggatgta cgcccgccgt tgaccaacag
    11401 gacgaatact gacgcgccag cttttgcgcc tgctggcttt cacgcaggtt agtgccggtg
    11461 atgagtagcc cattaactcc cgcgtcaaaa gcgcgcgcta caacatcatc acggtctttc
    11521 gcaaattgcg aactggtcaa attaacgccg atatcaaaca tcctgtactc catatgacaa
    11581 ccgccctgac gggcggttga atttattctt cagttttttc gctttctgct tcagcgtcgt
    11641 tttcctcttc ccgatttcgc cctttaccaa cgtaaaagcg tgagaagaag acaccgattt
    11701 caaacagaca gtacatcggg atcgccaata gcgtttgcga gaagacatcc ggcggcgtca
    11761 gcaacatccc gacaacgaat gcaccaacca gcacatacgg gcgttttttg cgtaagtctt
    11821 ctggcgaggt aatccccatc cagcacagca gcacaattgc caccggcact tcaaaggaga
    11881 caccaaacgc cataaacagc gccataacga agcttaaata gctggcgatg tcggtggata
    11941 cctgcacccc ttccggcgcg gtattggcaa ggaagccaaa tgccagcgga aagaccacaa
    12001 agtaggcgaa tgccatgccg atataaaaca gcagagagct ggaaaccagc agcggcacca
    12061 ccaggcgacg ttcatgctta tacagcgctg gggcgataaa tgcccacacc tgatagagaa
    12121 tcaccggcgc tgacagaatc agcgacacca taaaggtcag cttgatcggc gtaaagaacg
    12181 gcgaggccac gtcggtggcg atcatcgttg aaccttgcgg caactgcttg atcaatggcg
    12241 cggataccag gtgatagatg tcattggcga aatagaccag acacaggaat atcacgatca
    12301 ccgcgataat gcagttcagc agacgcttac gcagctcaat tagatgcgtg ataagcggtt
    12361 gagtatcttc tacagacatg tttacggttt atcactcgac gaaggggaag gtgcagcggt
    12421 tttcggttca gcgtccgcag caggttttac caccggctct ggcgtggttt ctggcttctg
    12481 ttccggcgaa ctggcctgcg tttgtgcagc ggcaggcgtt acgccctcat gcgcagcttc
    12541 attatctttc accaccgggt tatggatggt gtgcgcttca tcgctcgcct tttcaggatc
    12601 gtttgcaacg taggaacgct tcatcgactc cgcggcctgg cgtagttcat ccatcgacgc
    12661 tttcagttcg ggcgtcaggt tagtgaggct cgccttttca acctttttca gactgtcctg
    12721 aaactcctgg agttttaact cctgggtcag ttcgttctgc accgttgtcg ccagtgaacg
    12781 caacgcgcga atccagcccg ctaccgtttt taccgccaca ggcagtcgtt gcggccccag
    12841 aacgacgagg ccgatgatga acaccaatag cagttcgcta aaaccgatat caaacacgga
    12901 ttacacctgc tctttatcgt ggcgcttcgc gtcttctgtt ttagcctgtt cctgattcgt
    12961 atccgcctgc ttatcggcga tagttttcgc agtaaaatca gcatcctgac tggttttatc
    13021 ctgctttggt tcatcatcgc tcattgcttt tttaaagcct ttgatcgacg caccaagatc
    13081 ggaaccgatg gagccgagct ttttggtgcc aaaaagcagt acaacgatga cggcaataat
    13141 caataactgc caaatactga taccacccat acatgttcct ctgtggtaga tgatgattaa
    13201 acaaggccgc attatacgtt acacgacccg ccttgagcga tgaaaaaatc agcgtgtttt
    13261 gcgccaaccg acaaaccagg cgatcagacc acctgccatt aaccagccgg gcatcagccc
    13321 ccattcaggt cggctgacca acaagaatgt gccacttaat actaacgtag cgccaattcc
    13381 gagaaaataa cgcgattgtc cctgacgtac atgatttgac tgaagctcgc gggcaatctt
    13441 atcaacactg tgctgtaaat acttgccctg gcgcaaactg tcgtaaacca attcaggcag
    13501 ttctggcatt ttttcgaccc agaacggcgc tttttcttta aatgctctca ccagggcagg
    13561 aataccgacc tgatctttaa tccacgactc caggaaaggc ttcgctgttt tccataaatc
    13621 gagctgcgga taaagctggc gtcctacccc ttcgacatag agcagggttt tctggagtaa
    13681 caccagttgc ggctgcactt ccatattgaa gcgacgcgcc gtattaaaca gatttaacag
    13741 tacatgtcca aacgaaattt cggccagcgg tttctcaaag ataggttcac agaccgtacg
    13801 aatggcaaat tcgaactctt caacgttggt atctggtggc acccagccag aatcgacgtg
    13861 tagctctgcc actttgcgat agtcgcgatt aaagaaggcg ataaagtttt ctgccagata
    13921 gcgtttatct tctttgttta gcgagccaac aatcccgcaa tcaatgccga tatatttcgg
    13981 gttttccggg tgttcatagc ttacgaaaat gttgccaggg tgcatatcgg catggaaaaa
    14041 gctgtcgcga aagacctgag tgaagaacac ctgcacgcca cgttccgcca gcaatttcat
    14101 gttggtgcca tttttctcca gcgtcgcaac atcagacacc ggaatgccgt aaatgcgctc
    14161 catcaccatc atcccttcac tacaatagtc agggtaaact tccgggatgt agagcatcgg
    14221 gctgtcttca aaatttcgcc gaagctgaat ggcgttggca gattcccgca gcaaattcag
    14281 ttcatcaatc aaggtctttt cgtactcgcg caccacttcg gttgggcgca gacggcgacc
    14341 atccggcagc aaacgcggca cccagcgagc cagacggtag ataagtttca gatccgcttt
    14401 aataaccggc aaaatatccg ggcggatgac tttaatcacc acctctttac cattcgattt
    14461 caatcgcgcg gtatgaacct gggcgataga agcagaagcc agtggcttga tttcaaaatc
    14521 gtcaaaccac gcttctaccg gcaagccgcc cattgcagct tcaatctgct gcttcgccag
    14581 cttgccatca aacggagcaa ctttgtcctg caataacgcc agctgatcgg caatatgcgg
    14641 tggaaaaaga tcgcggcggg ttgataacat ttgcccgaac ttgatccaaa ccggccccag
    14701 ttcttgcagg gccagtcgta gtcgctcacc taaaagtttg tctttatgcc gatttggcat
    14761 ccagaataat gagtatcgcc atagccgtag cggcagggtg atacgcattt tggggatcag
    14821 ttcatcaagt ccgtagctta aaaaagtgcg aatgatgaaa tataggcgcc gtacttcacc
    14881 tggcgtcatt tagcctccag tttttccagc cgtttggtca gggcatcaac agcacgctcg
    14941 acggcagccg tctcttccgc aaaccaggcc acttcaagcg gaccgggtgc catacgccac
    15001 tcttcagtaa tggcttccgc cacataacgt tgctggcgct taatgccgtg atgcaggaac
    15061 tttgcgcctc cgcgcatggc tttgctgatt ccttcagcgg cgatatcacc ggtataaggg
    15121 gccagcagtt ccgcagggtc gaactctgcc agatctgcca gcgcaacgaa gttttgcacc
    15181 acctgaatat cgccctgcac ttccagctca ccactgcgaa tcagtgcggt aagctgctgg
    15241 cgatcgcgaa gtttcggcaa cacactggcg taggcgataa cggtgcagtc agcatcgcct
    15301 gcccattcgc ccagtacatc aacctggcgt tcgctgaaca ccagaatcaa tgacgtcgaa
    15361 aagcctttta cctccacgcg caatacttta cccagcagac gcgagcgggc tgttttcagc
    15421 gcgggtgagc gatacaggaa ggtgttgagc agactttcaa ttcctgccgt cactaaaggt
    15481 ttaaaaggca tttccggtct cctgtcagaa cttataacca cgatgcagcg ccacaacccc
    15541 tgccgtcaga ttgtagtagt cgacactttc gaatccggca tcctgcatca tggctttcag
    15601 ggtatcctga tcgggatgca tacggatgga ttctgccaga taacggtagc tgtcggcgtc
    15661 gttcgcgacc agtgagccaa tacgcggcag cacatggaag gagtatgcat cataggcttt
    15721 gctcagcggc tcgataattg gcttcgagaa ctcaagcacc agcaggcggc cgccgggttt
    15781 cagcacgcga tacattgaac gcagtgcttt atctttgtcg gtgacgttac gcagaccaaa
    15841 cgaaatggtg atgcaatcaa aggtgttatc cgggaacggc agcgcctcag cgttcgcctg
    15901 aacatactca acgttgccaa tcacaccgat attacgcagc ttctcgcgtc ccattttgag
    15961 catggattca ttgatatcag caaggaccac tttgccagtt tctccgacca ggcgggagaa
    16021 tttcgctgtc aggtcgccgg tgccaccagc cagatccagc acggtctgcc cacggcgtac
    16081 gccgctgcaa tcaatcgtga atcgcttcca caaacgatga ataccaaatg acatcaaatc
    16141 attcatgaca tcgtattttg atgccacgga atggaaaacg tgggcgacca tatccgcttt
    16201 ttgttccttc gcgacggtct gaaaaccaaa gtgcgtcgtt tcttgtgact tatccaccat
    16261 ctcaatgcct gctcatcaaa aaattgttcc agaagtgtaa cagattgggc gtcgatgccc
    16321 tagatttcta cccggcttaa ctacccctaa tgggctagcg cgactgctga ttatattcat
    16381 catcgcgttg ataagcttca tcattcggct gctccggaac cgaccgaagt cgatactctt
    16441 cattctggct caccgcctgt tcagccaaat ccggattaat ctcgcgttta atttctactc
    16501 ctaaaccgcg aaacgcttct gcctgcgcca gcacatttcc gcgacctgaa gagagttttt
    16561 tcattgcctg gcggtagtta tcctgcgctt tgtcgagact ttgaccaatc gcggacatgt
    16621 catcgatgaa caaacgcatc ttgtcgtaca gcttgctggc acgatcggcg atttgctggg
    16681 cgttgcggct ttgatgctca taacgccaca ggttggcaat agtacgcaac gccaccagca
    16741 gcgtagtcgg gctaaccagc atgatattgt ttttcaacgc ttcggtgatc agctccggct
    16801 ggcggtcaag cgccagtaaa aaagcgggtt caacgggaat aaacatcagc acgtaatcca
    16861 gagttcgcag ccccggcagc tgttgataat ctttgcgtcc cagcaaacgg atatggttac
    16921 gcaccgacgc gatatgttcc tgtagcgcgc tttcgcgggt gtagtcgtct tcggcgttaa
    16981 aatagcgttc ataggcgacc agcgtcattt tggcgtcgat caccacatct tttccctgcg
    17041 gcagccgcac gatgacatcc ggctgcatcc gcgagcgggc gtcattttcg atgctgacct
    17101 gggtttcata ttcataccct tcacgcagac cggaagcctc cagcacccgc gtcaatacta
    17161 cctcgcccca gttgccctgg gttttattgt cgcctttcag cgcgcgcgtc aggttgatcg
    17221 cttcctgggc catttgcgcg ttgagttgct ggagattgcg aatttcgtgg gtcagggtat
    17281 ggcgttcttg tgcttcttta ccgaagctgt cctgaacctg acggcggaaa ccgtccagtt
    17341 gttcacgtag cggcgacaac aggctgttca gactctgacg gttttgctca tcaacccggc
    17401 gattgctgtg ctcaaaaata cggttggcga ggttttcaaa ctgctcactg aggcgctgct
    17461 cgctgttaat catctggcga attttatcgt cagcatgttg ctgtgcggct tccatccgcg
    17521 tggttacttc acgcagatcg gcctccagag aggtgttaat actttgcagg ctgcgcactt
    17581 cgttattgag taactcgcac tctgcacgcc agtgctcgct ttgggtaatt tgttgttttg
    17641 ccgcgcttaa ctccgcgacc atctcttcac gttcagctaa ttgctcggct ttttgctgcg
    17701 catgttgata actggcaaac agccagccaa ttgccacacc caccaacgca ataactgcgt
    17761 aaaccatgat tgagaaatcc acaacgcctc ctgacgcaac aacttacccg cacaaaatag
    17821 aattgtgctg tacaaacgtc cagttggaaa ggcatttcct gcgaggcgct acgcaaaaat
    17881 acaaaaggcc gaacgcgtcg gccttcagac aggagaagag aattacagca gacgacgcgc
    17941 cgcttccacc acgattttca ccgcttggct ttcagtttgt ttcatcgtct cagcattcgg
    18001 gatctcttgc tgggtgcggt taacgataac acccgctacc ataccggcac gcaggccctg
    18061 acttgcacac atggtcagca gggttgcaga ttccatttca tagttcatta tgcccatcgc
    18121 ctgccactct tccatagaac ctttaaagtg acgaactacg cgaccagagt aagtatcgta
    18181 acgttcctga cctgggtaga aggtatcaga agaagctgtc acgccaacgt gagttgtcgc
    18241 gccaatggat ttcgcagctt caaccagcgc agtcgtacat tcgaaatcag cgacagccgg
    18301 gaattccagc ggtgcgaagt gcaggctcgc gccatccaga cggacagacg ccgtggtaac
    18361 caggacatca cccacattaa tatgcggctg aatagcgccc gttgtaccga tacgcaggaa
    18421 ggtgcgaatg cccagctgtg ccagctcttc aacagcaata gaggtagacg ggccgccgat
    18481 accggtagag cagacgataa caggtttacc atccagctct gcacgccagg tagtgaattc
    18541 gcggtgagat gccagcttaa ccggcttatc catcagcgcg gcgatctttt ccacacgatc
    18601 cgggtcgcca gggacgatgg caagcgtagc cccttgtaaa tcgtttttag tgaggccgag
    18661 atgaaaaaca tcagacttgg acatatacaa ctcctctgtg aatcggttta gtcagaagaa
    18721 gcaaaaagac actttaccga agggtttaac attttttcgt gatactcatc accatgacgc
    18781 aaatgcgttg cataaattta cccaccaaaa gtgattcaaa tcacataaat cactcttcat
    18841 tctgcaggac tgcataaaag atgagccgat tcaggcaatg tgatttgttg catgctgact
    18901 atagttaaca ttgcgcaaca tttttataag ggtacggaga ataccatggc aacaacacaa
    18961 caatctggat ttgcacctgc tgcatcgcct ctcgcttcga ccatcgttca gaccctggac
    19021 gacgcgattg tggcgggctt cacctctatc ccttcacaag gggataacat gcctgcttac
    19081 catgccagac caaagcaaag cgatggccca ctgccagtgg tcattgtagt gcaggaaatt
    19141 tttggcgtgc atgaacatat ccgcgacatt tgtcgccgtc tggcgctgga ggggtatctg
    19201 gctatcgcac ctgaacttta cttccgcgaa ggcgatccga atgattttgc cgatatcccc
    19261 acgttgctta gcggtctggt agcaaaagtg cctgactcgc aggtgctggc cgatctcgat
    19321 catgccgcca gttgggcttc ccgcaacggc ggcgatgttc atcgtttaat gatcaccgga
    19381 ttctgctggg gtggacgtat cacctggctg tatgccgcgc ataatccaca gctaaaagct
    19441 gcagtggcgt ggtacggcaa actgacgggc gacaagtcgc tgaattcacc gaaacaacct
    19501 gttgatatcg caaccgatct taacgcgccg attctcggct tatatggcgg tcaggataac
    19561 agcattccgc aggagagcgt tgaaaccatg cgccaggcgc tgcgggctgc taatgcgaaa
    19621 gcagagatta tcgtgtaccc ggatgccggg catgcattca acgctgatta tcgcccgagc
    19681 tatcatgccg aatctgcgaa agatggctgg cagcgtatgc tggaatggtt taagcagtat
    19741 ggagagaagc ggagttagtt tcctcttagt ctatatttga gatggtgagt aaacgagtgc
    19801 gttttgctgc catctctgtc ccccaaaaaa taatcagtat gaattgattg gagattacga
    19861 ctccgctatt tgaccttaag aaagtcggca agtcatagtc ataaatgagt ctcttcaatg
    19921 gaagacacct tagagattta ccatcaactc ccattcacaa acaaacatta attattatca
    19981 acacattcac attaattata aatcatctca gaccttcctg ataaaaagat attaaagtga
    20041 aacattttgt ttttcacatt ttagttcatc acttttaggc gtaaataaag gtaaaaaaaa
    20101 caacaatact atttctcttt cacaaaagaa ttaacaaaag gtttttcaag gatgagtgat
    20161 attatttacc tgaccataac cggagaacaa caaggatgta tttcctctcg ttgtggaacg
    20221 agcgcatcaa taggtaatcg ctggcagatt ggccatgatg atgaaatctt cgcgttctcg
    20281 ctctctaata gtattacgaa tacaggcaaa gggtcataat tgcatggcct tagtttctgt
    20341 aaattgattg acaaaagctc cccgttactt attaacgcta ttaacaataa cgagcagttg
    20401 ttcatggagt ttgacttcta tcggatcaat aggtttggtc gatgggagaa atattacaac
    20461 attcaactcc gcggcgcgct cttatcagca atcaatcacc tgtttactga gaacaatctg
    20521 gatacagaag ccatcactgt cagttattaa tatatactct ccaggcatct gattgctaat
    20581 actgaattta gttattgggc atttcccgat aactacaacc gtctgtttat accacggcca
    20641 aaaactaaag ctggtaatgg tttcaaaaca cttaatagta aagcggttgg cagactgctt
    20701 gctgctgtcg gcatatacaa tgggaatatc gaaggattca gggaaacagc agacaagttg
    20761 gggggagatg cacctgctgg ttatgatcaa tttatggata acaaagggtt actcatcgct
    20821 ggtgcttcaa tagttgctgg agcagttctg gggagaatga gactccctga tttggaatca
    20881 cttgagcatt tcggagccag aggagcagta tcaggaagac catttaatcc cgagcttgcc
    20941 ggtggtccaa tagaaaacct gacgacagat ggcgtaacaa tcaacaggga aggtatcgct
    21001 attgttgaaa aacatatagc cagatttggt catgatcctg taaatgaagt gatgatcaat
    21061 agactgaaag acattgagaa aggaaaaatc cccccagaac aagtagatct taacttctat
    21121 actcatgagt gtcgggaata tcagcgttac tgtaatcttg gttgggaaac aggacaacct
    21181 gatggtgacg caggctatgc actctggaat catactggat ttgcccctat atttccagac
    21241 atctgttatc acttaaccca ttacaagccc gctgcc
//
LOCUS       DAJTVJ010000072        21111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000072 DAJTVJ010000000
VERSION     DAJTVJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.072"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1278)
                     /locus_tag="OO448_002582"
     CDS             complement(<1..1278)
                     /locus_tag="OO448_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337764.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAKR"
     gene            complement(1471..1540)
                     /locus_tag="OO448_002583"
     tRNA            complement(1471..1540)
                     /locus_tag="OO448_002583"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1506..1508),aa:Gly,seq:ccc)
     gene            complement(1619..2374)
                     /locus_tag="OO448_002584"
     CDS             complement(1619..2374)
                     /locus_tag="OO448_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6337765.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2789..5086
                     /gene="xdhA"
                     /locus_tag="OO448_002585"
     CDS             2789..5086
                     /gene="xdhA"
                     /locus_tag="OO448_002585"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6337766.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5097..5975
                     /gene="xdhB"
                     /locus_tag="OO448_002586"
     CDS             5097..5975
                     /gene="xdhB"
                     /locus_tag="OO448_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6337767.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGSAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5972..6451
                     /gene="xdhC"
                     /locus_tag="OO448_002587"
     CDS             5972..6451
                     /gene="xdhC"
                     /locus_tag="OO448_002587"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6337768.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6491..8269)
                     /locus_tag="OO448_002588"
     CDS             complement(6491..8269)
                     /locus_tag="OO448_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR6337769.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            8748..9935
                     /gene="ygeW"
                     /locus_tag="OO448_002589"
     CDS             8748..9935
                     /gene="ygeW"
                     /locus_tag="OO448_002589"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6337770.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9993..11189
                     /gene="dpaL"
                     /locus_tag="OO448_002590"
     CDS             9993..11189
                     /gene="dpaL"
                     /locus_tag="OO448_002590"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR6337771.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            11247..12458
                     /locus_tag="OO448_002591"
     CDS             11247..12458
                     /locus_tag="OO448_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR6337772.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            12511..13895
                     /gene="hyuA"
                     /locus_tag="OO448_002592"
                     /pseudo
     CDS             12511..13895
                     /gene="hyuA"
                     /locus_tag="OO448_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            13943..14875
                     /gene="arcC"
                     /locus_tag="OO448_002593"
     CDS             13943..14875
                     /gene="arcC"
                     /locus_tag="OO448_002593"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6337773.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(14916..16541)
                     /locus_tag="OO448_002594"
     CDS             complement(14916..16541)
                     /locus_tag="OO448_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR6337774.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(16589..17359)
                     /gene="yqeC"
                     /locus_tag="OO448_002595"
     CDS             complement(16589..17359)
                     /gene="yqeC"
                     /locus_tag="OO448_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR6337775.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            17462..18040
                     /gene="mocA"
                     /locus_tag="OO448_002596"
     CDS             17462..18040
                     /gene="mocA"
                     /locus_tag="OO448_002596"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR6337776.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            18362..20158
                     /gene="ygfK"
                     /locus_tag="OO448_002597"
                     /pseudo
     CDS             18362..20158
                     /gene="ygfK"
                     /locus_tag="OO448_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
     gene            complement(20164..>21111)
                     /locus_tag="OO448_002598"
     CDS             complement(20164..>21111)
                     /locus_tag="OO448_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR6337777.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
                     LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWG
                     VLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDL
                     ASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         5402 a         5463 c         4990 g         5256 t
ORIGIN      
        1 tcttttcgca tgtgaatttt gatcggtatc ggttttaata tcattgaacc aaaaatttgc
       61 cgactgataa ttatccttct cgtatctttt tataccatac ggacctgcta gttttttaac
      121 tatttttaga acctgctctt ttcttctggt gtttatcttt gcaagatttg caggataaat
      181 aacattaagt aaagccgcat cggcttctct gtaatgttta tctttttcca gataacctgg
      241 ggattctccc cctaaatcaa gctgtaacgt tattctttca taacctttat ctatgaggtg
      301 gttcaacctc gttgttgaaa gaggttcatc cagttcattg gctttagctt cgcgtagtaa
      361 atcagagaca aaaacagagt ctttcttcga gagtaagttc gacaaacgtt ccagtccaga
      421 ggtcaccagt gcaacggaag atgtgtttaa tcgggcatct tcttcccatg ctccggaatc
      481 ttccataaca tagaaattgg ctttatccag ataagcaatc agcagcgcaa ccgattttaa
      541 tcggtcacct ttttgccaat cttcagcgtt aattgtacca gtgtcgattg cttgtataag
      601 gagatcaaga taaagaccta acgcatcatt ttgtttatgg ttccacagct gcggcttgcc
      661 ttcttcctgc acatctgcca tcacggggga gttgctgtca aatcggatat gcacagcatt
      721 catttgcccg ggaactccat ccagtcgttt ggggttgcta atgacgtcct gcatccgctt
      781 aatctggtcg agcgtagaca tataatccca cagggtgagc agaacctttt ttgcggctac
      841 actatttccc tgatctgata ctaatgccat atagccccat aaactatccc tcagccagac
      901 agcatcatag tgggtttctg ccgctgtttt gtcatcttcg aaagtagaag caagaataaa
      961 tgttccccag ggtttctccg tcaacgttaa ctttcgtgtg ttttctgtta atgttaacaa
     1021 cccttgaatt tcgtctttgg tatagtggct ctttatttca ttttttaatt tggctgagtg
     1081 aatcagaaga cgataattag ggtacttatc tggatttaac gataaataac ttttatctgt
     1141 tgctgccccc gtggaattaa gaatgttaaa tacagattca ctcgtcactt ctgcccctgt
     1201 cgcctggcat gtgccgcacg aaaatatacc agcgagcaga agagttaatg cagatttccc
     1261 tttcataaaa tattccatca atcgtttgtt aaaagcgtaa ataaccactt atttaacaac
     1321 ttgaaaataa ggtgttttac ctgggttgtt acaaaaggat tgcattgcgt aaacgctttt
     1381 tatttacaac aaaatgggga aatattacgg cgattgcgaa aagtctgaag cgattgaatt
     1441 aagataatat agaaaatcag atacagcaaa agcggcgaag ggaatcgaac cctcgtatag
     1501 agcttgggaa gctctcgttc taccattgaa ctacgcccgc ttcgagatgc gtaaggcatt
     1561 ataaacctta cgctctcctt agcaagtgcc gcgctgctga ctgctgatta attcgccatc
     1621 agcattttgg cttgctgccc tgcggcggca agtaacgtag cggatcaatt gccgttgcac
     1681 ggtaacgaat ctggaaatgc aggcgaacag atgccgcatc cgtgctcccc atagtggcga
     1741 ttttttgccc agccttcacg ctttgcccat tatttaccag catcgtgtca ttatgggcgt
     1801 aagccgtaat gtaatcttca ctgtgtttaa tcatgatgag attaccgtag ccacgcagct
     1861 ggttgcccac atacaccacc tttcctgcac ccgcggcgta aataggtgta ccccgtggag
     1921 ctgagatatc aatcccttta ttgccgccat ctgctgtcga atacggcatg ataactttcc
     1981 ctgtcgttgg ccataaccaa caacgttgcc ctactggcgg ccaggatgat ttcggtaccg
     2041 ctgatgacgg tgtaaccgat gcggttttgg tcgttgattt ggcggttgat ttacgtgtac
     2101 tactgctact tttcgcccca cccagtttta gtttctgacc aacttcaatg gtgtaagggg
     2161 gggaaatgcc gttcagtcgc gccagttctt ttacgctggt tcccgtggtg cgcgaaatac
     2221 gatatagcgt atccccccgt ttcacggtgt aaacggagcc ggaatacgtt cctgtatcgg
     2281 atgatttgct acccgaacag cccgccaaaa gcagtccaac cgataacaac atcacgatac
     2341 ccagagattt tttattcagg cgtcccgcac tcaaaatctt tcctcgctaa aaaaacaaga
     2401 cgccttatca tagcagccag cctcttgata cccaatctct tgcttctgga aaaacgtggt
     2461 aatagagaaa gaggttaatt tcatttggca aagggaagta tcatcagcaa aatattcgct
     2521 gtagaggtat cgaacttcct gactttttac cctatttccc atcctgatgc gtaataatcc
     2581 caatttatca cattgatttt atcgccactc atattgatac gtatcactgc ccctattcag
     2641 agtcctgttt cgtatcatat ctgtgttttt ttaacgtcat cacacttcct acctccccta
     2701 acgcttatcg tcgtttctgg cgtaaatctt gcctgcttag actaaatctt tgccataaga
     2761 accaaatgtt caaatcgagg ggatttctat ggaagcgcgg gaagcaaccg ctacgggtga
     2821 atcatgcatg cgcgtcgatg ccattgctaa ggtcaccggg cgggcacgat atactgacga
     2881 ttatgttatg gcgggcatgt gttatgcgaa atatgtacgt agccctatcg cacatggtta
     2941 tgccgtaagt attaatgatg aacaagccag aagtttgccg ggcgtactgg cgatttttac
     3001 ctgggaagat gtgcctgata ttccattcgc tacagctggg catgcctgga cacttgacga
     3061 aaacaagcgc gataccgccg atcgcgcact gctaactcgc catgttcgtc atcatggtga
     3121 cgccgttgcc atcgtcgtgg cccgcgatga actcacggca gaaaaagcgg cgcaattggt
     3181 cagcattgag tggcaagaat tacccgttat caccacgcca gaagcggcgc tggcagaaga
     3241 cgctgcacca atccataacg gtggcaattt actgaaacaa agcacgatgt cgacgggtaa
     3301 tgtccaacaa acaatcgatg ccgccgacta ccaggtacag gggcactatc agacccccgt
     3361 tattcaacat tgtcacatgg aaagcgtaac atcgctggcg tggatggagg atgactcgcg
     3421 aattaccatc gtttccagca cccagatccc gcacattgtt cgccgcgtgg ttggtcaggc
     3481 gctggatatt ccctggtcat gcgtacgagt catcaaaccg tttgtcggtg gcggttttgg
     3541 taataaacag gatgtactgg aagagccaat ggcagcattc ctgaccagca agcttggcgg
     3601 cattccggtg aaagtttccc ttagccgtga agagtgtttc ctcgcaaccc gtacccgcca
     3661 cgcttttacc attgacgggc aaatgggcgt gaaccgcgac ggaacattga aaggttatag
     3721 tctggatgtt ctgtctaaca ccggcgctta tgcatctcac gggcactcca tcgcttctgc
     3781 ggggaggaat aaagtcgctt acctttatcc tcgttgtgcc tacgcttaca gttcaaagac
     3841 ctgctatacc aacctcccct cggctggtgc gatgcgtggt tatggcgcgc cacaagtcgt
     3901 atttgccgtt gagtctatgc ttgatgacgc cgcgacagcg ttaggtattg atcctgttga
     3961 aattcgttta cgcaacgccg cccgcgaagg agatgctaat ccgctcacgg gcaaacgtat
     4021 ttacagcgca gggttgccgg agtgtcttga aaaaggccgg aaaatctttg aatgggaaaa
     4081 acgccgtgca gaatgccaga accagcaagg caatttgcgc cgcggcgttg gcgtcgcctg
     4141 ttttagctat acctctaaca cctggcctgt cggcgtagaa atagcaggcg cgcgccttct
     4201 gatgaatcag gatggaacca tcaacgtgca aagcggcgcg acggaaatcg gtcagggtgc
     4261 cgacaccgtc ttctcgcaaa tggtggcaga aaccgtgggg gttccggtca gcgacgttcg
     4321 cgttatttca actcaagata ccgacgttac gccgttcgct cccggcgcat ttgcctcacg
     4381 ccagagctat gttgccgcgc ctgcgctgcg cagtgcggca ctattattaa aagagaaaat
     4441 catcgctcac gccgcagtca tgctacatca gtcagcgatg aatctgaccc tgataaaagg
     4501 ccatatcgtg ctggttgaac gaccggaaga gccgttaatg tcgttaaaag atttggcgat
     4561 ggacgctttc taccaccctg aacgcggcgg gcagctctct gctgaaagct ccatcaaaac
     4621 caccactaac ccatcagcgt ttggctgtac ctttgttgat ctgacggtcg atattgcgct
     4681 gtgcaaagtc accatcaacc gcatcctcaa cgttcatgat tcagggcata ttcttaatcc
     4741 actgctggca gaaggtcagg tacacggcgg aatgggaatg ggcattggct gggcgctatt
     4801 tgaagagatg atcatcgatg ctaaaagcgg cgtggtccgt aaccccaatc tgctggatta
     4861 caaaatgccg accatgccgg atctgccaca actggaaagc gcgttcgtcg aaatcaatga
     4921 gccgcaatcc gcatacggac ataagtcact gggtgagcca ccaataattc ctgttgccgc
     4981 tgctattcgt aacgcggtga agatggctac cggtgttgca atcaatacac tgccgctgac
     5041 gccaaaacgg ttatatgaag agttccatct ggcaggattg atttgaggat aacatcatgt
     5101 ttgattttgc ttcttaccat cgcgcagcaa cccttgccga tgccatcaac ctgctggctg
     5161 acaacccgca ggccaaactg ctcgccgatg gcactgacgt actgattcag ctccaccatc
     5221 acaatgaccg ttatcgccat attgttgata ttcataatct ggcggagctg cggggaatta
     5281 cgctggcgga agatggctcg ctacgtatcg gctctgcaac gacatttacc cagctaatag
     5341 aagctcctat aactcaacgt catctcccgg cgttatgtgc tgcggccacg tccattgctg
     5401 gaccgcagat ccgtaacgtc gctacctacg gtggaaatat ttgcaacggt gccaccagcg
     5461 cagattctgc cacgccaacg ctaatttatg acgcgaaact ggagatccac tccccgcgcg
     5521 gtgttcgttt cgtcccgatt aatggctttc acaccgggcc gggcaaagtg tctcttgagc
     5581 atgacgaaat cctcgtcgcc tttcattttc cgccacagcc gaaagaacac gcgggcagcg
     5641 cgcattttaa atatgccatg cgcgacgcaa tggatatttc aacgattggc agcgccgcac
     5701 attgccgact ggataacggc aatttcagcg aattacgcct ggcatttggt gttgccgcgc
     5761 caacgccgat tcgctgccaa catgccgaac agactgcaca aaatgcgcca ttaaacctgc
     5821 aaacgctgga agctatcagc gaatctgtcc tgcaagatgt cgccccgcgt tcttcatggc
     5881 gggccagtaa agagtttcgt ctgcatctca tccagacgat gaccaaaaaa gtgattagcg
     5941 aagccgtcgc cgcggcgggg ggaaaattgc aatgaatcac agcgaaacaa ttaccatcga
     6001 atgcaccatt aacgggatgc cttttcagct tcacgccgca ccaggcacgc cgctctcgga
     6061 attactccgc gaacaaggac tgctaagtgt caaacaaggg tgctgcgtgg gtgaatgtgg
     6121 tgcctgtacg gtgttggtcg acggcacagc aatagacagt tgcttatacc ttgccgcctg
     6181 ggctgaagga aaggagatcc gcacgctgga aggtgaagcg aaaggcggaa aactttctca
     6241 tgttcagcag gcttatgcga aatccggcgc agtgcagtgc gggttttgta cgcctggcct
     6301 gattatggct accacggcaa tgctggcgaa accacgcgag aagccattaa ccattacgga
     6361 aattcgtcgc ggactggcgg gaaatctttg tcgctgcacg gggtatcaga tgattgtaaa
     6421 tacagttctg gattgcgaga aaacgaagta aaaggatatc cggcctgaat tcaggccgga
     6481 ttcactgagg ttatgtgttt aacaactcat atttcttaat cttgcgatag agcgtagcaa
     6541 tgccgatgcc cagttcatca gcaacttgct tcttgctgtt atgacgtgaa agcgcctcgc
     6601 ggatcatttg cttttccatc tcctccagcg ccgtgccgcc cgcatcatcg agtgacaggt
     6661 gcgcctcact gacctctgtt acatcacttt gctccgttgt gccattattc agcagatttg
     6721 gcggcaatag cgtgctgtcg ataacttcac ctgaaggaac cacgttaacc agatattcca
     6781 tcaaattgct taactcgcgc aggtttccgg gccaacgatg cttacgcaat atttcgacga
     6841 catcgggagc aatgccagga taaaccgatc ccagacgacg ggtatgcaga tgtaaaaagt
     6901 aatgcaccaa tagttcaata tcttcctgac gttcacgcag cggtggcaga gttatcggga
     6961 taacattaag tcggtagaag agatcttcgc ggaatttacc ttcggcaatg aactgggcca
     7021 aattctgatt agttgcagaa atgatgcgaa tgtcgacttg tattgggcta ctggcaccaa
     7081 tcggcagaat ttcacgcgcc tcaatagcgc gcagtaattt agcctgcaac attaatggca
     7141 tatcacctat ttcatcgaga aacagcgtgc ccgtattcgc cgcctgaatc aaccctgttt
     7201 taccgttggc agaagcacca gtaaatgcgc ctttaacata accgaacagt tcgctttcca
     7261 gaagctgctc cggaatcgcg gcacagttga tagcaataaa gggtttattc cgtcttccgc
     7321 tcaacttatg gattgctcgg gcgacgactt ctttacccgt gccgctttca ccaaccacca
     7381 taacgctgga tgggctgggt gcaatacggc taatgagtcg ttttaattgc cgcataacac
     7441 ggcactcgcc aaccaattgt tcaatatgtg gttcatcagg tgcatttgct acagaaaaac
     7501 tggtatgcga ttggtgaaat gccattaaaa ataattgtcg gccctgaatg ttatgcaatt
     7561 gaccaatgat taattcactt ttatcgtccc acgaaacaat atgctgcata tgtccatgag
     7621 taaaattact ctcaaatgtt aatggtctga aacggatagg tttcccaata atattatttt
     7681 gtacaacacc aagtgttttt aaggcagtct gattaacaaa ctgaacccga ctttcatcat
     7741 caacaactaa tacgccctga tccatattat cgatcatggt cgcgaatatt ttactgatgt
     7801 tatctcctgg cccctgatcc tccagaagtt tcgaaacaaa aatggtggat atatggcgaa
     7861 cataatcaga aaattcgcgt aaattatcac tgatatgctc ttgttgctcg tgggtaacgg
     7921 caatcaaact tatcacccca acacaacgat cctgtaaaat gacaggcgta cccagaaatg
     7981 ctttttcgcg gcaattttct ttactatcgc aaccttcgca aaggggatcg aagcgagact
     8041 gtgtcacaac tttttcagtt ttcgtttcca ggacgtggcg gagcaggcgt gagttgccgc
     8101 tcaactggcg accaagaaac ttcccatacg cgcccgttcc ggcgacgcga cacaagtttt
     8161 catcaacgat ctcaacctca aactgcaaaa cgctggcaag cattctggca aaacgctgaa
     8221 ttgtcggttg aatttgcatc aatactgact gcgtagtagc aagctccata gctttacctt
     8281 ccagacttac ttaaaagtcg atcattgaag acgttgatgg ttcacagatc atgatgatat
     8341 taactcaggc gaaattggct ttgataaaaa cataagattt ttatcatttt ctaatgcaat
     8401 tatggaagag atatcacatt tctatatcaa tatgagaatt acggcggtga gtttatcaaa
     8461 ctgaagagag atagcctgcc cctttatctt atttctgata cttagcagaa aataaataac
     8521 gcgataaaaa aagccaaacg ttttcgtatt ttacaaacaa ccagaagctg gcatcaattt
     8581 gtgaacaacc ccacacatta tccgtcaaat tagtcttttg cagccgcgcg gataattctg
     8641 gcacacttat tgttagtccc aggtatagct atgaaaacac caatcacttt ggcaagtcac
     8701 agtgaaataa accactttgc ctgtcattcc actaccggga ctgtatgatg aaaactgtta
     8761 atgagctgat taaggatatc aattcgctga cctctcacct tcacgagaaa gattttttgt
     8821 taacgtggga acagacgcca gatgaactga aacaagtact ggacgttgcc gcagcattaa
     8881 aagcactgcg tgctgaaaac atctcaacca aagtctttaa tagtggatta ggtatttccg
     8941 tattccgcga caactccacc cgtacccgct tctcttatgc ttccgcgctt aacctgctcg
     9001 gccttgcaca acaagatctc gacgaaggca aatcacaaat cgctcacggc gaaaccgtgc
     9061 gtgaaaccgc caatatgatc tccttctgcg ccgacgctat tggtattcgc gacgatatgt
     9121 atctgggcgc aggcaacgcc tatatgcgtg aagttggcgc tgcacttgat gacggttaca
     9181 agcagggtgt actgccacag cgtccggctt tagtgaacct gcaatgcgat attgaccacc
     9241 cgactcagtc aatggctgac ctcgcgtggt tacgtgaaca ctttggttca ctggaaaacc
     9301 tgaaaggtaa aaaaatcgcc atgacctggg cctactctcc aagctatggc aaaccgctct
     9361 ctgtaccaca aggcatcatc ggtctgatga ctcgcttcgg tatggatgtc accttggccc
     9421 atccggaagg ctacgacctg atcccggatg tggttgaagt ggcgaaaaac aatgctaaag
     9481 cctccggtgg tagcttccgt caggtcacca gcatggaaga agccttcaaa gacgcagaca
     9541 tcgtttatcc gaagtcatgg gcaccttaca aagtgatgga agagcgtact gaattgctgc
     9601 gtgcgaacga ccacgaaggc ttaaaagcac tggaaaaaca gtgtctggca cagaacgcgc
     9661 aacacaaaga ctggcattgt actgaagaga tgatggaact gacccgtgat ggcgaagccc
     9721 tgtacatgca ctgcctgcca gctgatatca gcggcgtatc ctgcaaagaa ggtgaagtga
     9781 ctgaaggcgt attcgaaaaa taccgtatcg ctacctacaa agaagccagc tggaagcctt
     9841 atatcatcgc cgcgatgatc ctgtcccgta aatacgccaa accaggtgca ctgctcgagc
     9901 aactgctgaa agaagcgcaa gaacgcgtga aataacccct cgggccgatc tgctggtcgg
     9961 ccctctattt ccagaggcca aaaggatagg atatgtccgt tttctcattg aagattgata
    10021 tcgccgataa caaatttttc aacggcgaaa catcaccgct cttttcgcaa agccaggcca
    10081 aactggcgcg ccagttccac cagaaaatag ctggttatcg cccaacaccg ctttgtgcgc
    10141 tggacgatct cgcaaacctt tttggtgtga agaaaattct cgtcaaagac gaatcaaaac
    10201 gattcggtct gaacgccttc aaaatgcttg gcggtgcgta cgccatcgct caattattgt
    10261 gtgaaaaata tcatcttgat atcgaaacgc tgtcatttga gcacctgaaa aatgccatcg
    10321 gcgaaaaaat gactttcgcg accaccaccg acggcaacca cgggcgcggt gtggcgtggg
    10381 cagcacagca actcggacag aatgcggtga tttacatgcc gaaaggttct gctcaggaac
    10441 gcgttgacgc cattctgaac ctcggtgccg agtgcatcgt cacggatatg aactatgacg
    10501 ataccgttcg cctgaccatg caacacgcgc agcagcacgg ctgggaagtg gtacaggaca
    10561 cggcatggga aggttacacc aaaatcccaa cctggatcat gcaaggctac gcaaccctgg
    10621 cagatgaagc cgtcgagcaa atgcgtgaaa tgggcgtaac cccgacgcac gttctgctgc
    10681 aagccggtgt cggagcaatg gccggtggtg tgctgggtta tctggtcgac gtctacagcc
    10741 cgcaaaatct gcacagcatt attgttgaac ctgacaaagc tgactgtatt tatcgctccg
    10801 gcgtcaaagg cgacatcgtc aacgttggcg gtgatatggc caccatcatg gcgggcctgg
    10861 cctgtggcga acctaacccg ctgggctggg aaatcctacg taactgcgcc acccaattca
    10921 tctcctgcca ggacagcgtt gccgcattag gtatgcgcgt gctgggtaat ccgtacggca
    10981 acgacccgcg catcatctcc ggtgaatccg gcgctgtcgg tttgggcgtt ctcgcagcgg
    11041 ttcattatca cccgcaacat caaagcctga tggaaaaact ggcgctggac aaagatgccg
    11101 tagtgctggt tatcagcact gaaggcgaca ccgacgtgaa gcactaccgc gaagttgtct
    11161 gggaaggcaa acacgctgta gcaccttaat caccctattg gaactactcc ctgcaaaacg
    11221 gggagtaaaa aatctggaga aaaataatgg ctaagaatat tccattcaaa ctgattcttg
    11281 aaaaagcaaa agattaccag gcggacatga ctcgcttcct gcgcgacatg gttgctattc
    11341 ccagtgaaag ctgcgacgaa aaacgcgtag tacatcgtat taaagaagag atggaaaaag
    11401 tcggcttcga taaagttgaa atcgacccga tgggcaacgt tctcggttat atcggccacg
    11461 gcccgcgtct ggtggcaatg gacgctcata tcgataccgt cggcattggc aacatcaaaa
    11521 actgggactt cgatccgtac gaaggcatgg aaactgatga gctaatcggt ggtcgcggta
    11581 cttccgacca ggaaggcggc atggcatcta tggtttatgc cggtaaaatc attaaagacc
    11641 tcggtctgga agatgaatat accctgctgg ttaccggtac tgtgcaggaa gaagactgcg
    11701 acggtctgtg ctggcagtac attattgaac aatccggcat tcgcccggaa tttgtggtca
    11761 gtaccgaacc aaccgactgc cagatatacc gtggtcagcg cggtcgtatg gaaattcgta
    11821 ttgatgttca gggtgttagc tgccacggtt ctgcgccaga acgcggtgac aacgccattt
    11881 tcaaaatggg tccgattctt ggcgaattac aagaactctc ccaacgtctg ggttatgacg
    11941 aattcctcgg caaaggcacc ctcaccgttt ctgaaatctt cttcacatcc ccaagccgtt
    12001 gcgctgtagc agatagctgc gccgtctcta ttgaccgccg tctgacctgg ggcgaaacct
    12061 gggaaggcgc gctggacgaa atccgcgccc tgcctgcagt acagaaagct aacgcggttg
    12121 tttctatgta caactacgac cgtccgtcct ggactggcct ggtttaccca accgaatgct
    12181 acttcccgac ctggaaagtg gaagaagatc acttcaccgt taaagcactg gtgaatgcct
    12241 acgaagggct gtttggcaaa gcgccggttg ttgataagtg gaccttctca actaacggcg
    12301 tatctatcat gggccgtcac ggcattccgg tgatcggctt tggcccgggt aaagaacctg
    12361 aagcgcatgc acctaacgaa aaaacctgga aatctcacct ggtgacctgt gccgcgatgt
    12421 acgctgcaat cccgttaagc tggctggcaa cagagtaatt actcttcact tattcccctc
    12481 cggttcgccg gagggttttt ggagtttgct atgcgcgtat tgatcaaaaa cggcactgtc
    12541 gttaacgcag atggacaagc caaacaggat ttgctgattg aaagcgggat tgttcgccag
    12601 ttgggcaaca atatttcgcc gcagctcccg tatgaagaaa ttgatgccac tggctgttac
    12661 gttttccctg gcggcgtgga tgtccatacg catttcaata ttgatgtcgg catcgcgcgc
    12721 agttgtgatg attttttacc ggtacccgcg cagctgcgtg tggcggtaca acaaccatta
    12781 ttgaccatat gggatttggc ccaaacggct gtcggttacg ccatcaactg gaggtttatc
    12841 gtggttatgc cgcccataaa gcggtcatcg attacagctt tcacggtgtg atccagcaca
    12901 ttaatcacgc aatcctcgac gaaatcccga tgatggtcga ggaaggactg agcagtttta
    12961 aactctattt aacctatcaa tacaaactca acgatgacga ggttttgcag gcattacgcc
    13021 gcttgcatga atccggcgcg ctgaccaccg tgcacccgga aaatgatgcg gctatcgcca
    13081 gcaagcgggc ggaatttatc gccgcagggt taaccgcgcc gcgctatcat gccttgagtc
    13141 gccctctgga atgcgaagcg gaagccatcg cccgcatgat taacctggca caaattgccg
    13201 gtaacgcccc gctctatatc gtgcacctgt ctaacggctt aggtctggat tatctgcgtc
    13261 ttgcccgtgc gaatcaccag ccagtctggg ttgaaacctg cccacaatat ctcctgttgg
    13321 acgaacgcag ttacgataca gaagatggca tgaagttcat tcttagccca ccgctgcgta
    13381 acgtacgcga gcaggacaaa ctgtggtgtg gcatcagcga tggtgcgatt gacgtggtgg
    13441 cgaccgatca ctgcaccttc tcgatggctc aacgcctgca aatttctaaa ggcgatttca
    13501 gtcgctgccc aaatggctta cccggtgtgg aaaaccgcat gcagttactg ttttccagtg
    13561 gcgtgatgac gggacgtata acaccggaac gctttgttga attaaccagc gcaatgcccg
    13621 ccaggctgtt tggcctgtgg ccgcaaaaag gattattagc gcccggttcc gatggcgacg
    13681 tggtgattat cgacccacgt cagagccaac aaattcagca tcgccatctc cacgacaacg
    13741 ccgactactc gccatgggag ggttttacct gtcagggcgc gattgtcaga accttatccc
    13801 gtggtgaaac gattttctgt gacggcacct ttacaggcaa agccgggcga ggtcgtttcc
    13861 tgcgacgcaa accgtttgtc cctcccgtgc tctaaccact gtcagggatc tatacacaat
    13921 caagaatgat gagtgaggaa aaatgagtaa gaaaattgtt ctcgccctgg gcgggaatgc
    13981 gctgggcgat gacctggccg gacaaatgaa agcggtaaaa attacttctc aggcaattgt
    14041 tgatttaatt gcccaaggac atgaagttat cgtcactcat ggtaacgggc cgcaggtggg
    14101 catgattaac caagcctttg aagctgccgc aaaaactgaa gcacactcgc caatgctgcc
    14161 gatgtctgtc tgtgttgcct taagccaggg ttatattggt tacgatctac aaaacgcgtt
    14221 aagagaagaa ctgctttctc ggggcattaa taaacctgta gcaacgctgg ttactcaggt
    14281 cgaagtcgac gctaacgatc cggcattcct caacccgacc aagccgatcg gttcgttctt
    14341 taccgagcaa gaagcagagc aactgacaaa acagggttac actctgaaag aagatgctgg
    14401 tcgcggctat cgccgtgttg ttgcctcgcc aaaaccggtt gatatcattg aaaaagaaac
    14461 ggttaaagct ctggtagatg caggccaggt ggtgatcgcc gttggcggtg gtggtatccc
    14521 tgttattcgt gaaggtaacc atctgcgtgg tgccagcgcg gttatcgata aagactgggc
    14581 cagcgcccgt ttagcagaaa tgattgatgc cgatatgctg atcattctga ctgcggtaga
    14641 aaaagtggcc attaacttcg gtaaagagaa tgaacagtgg ctcgatcgcc tgtcattaag
    14701 tgatgctgaa cgctttatcg aagaaggtca ttttgcgaaa ggttctatgc tgcctaaagt
    14761 ggaagctgcc gcctcgtttg ctcgctctcg cgcaggtcgt gaagcactga ttaccgtatt
    14821 gagcaaagcg aaagaaggga ttgaaggcaa aaccggaacg gtgatttgtc agtaatttta
    14881 aggccgggtg ggtaatacca tccggccatt ttcatttaag ccacttccag cacttctttt
    14941 gtcgctttca cgccctgatg catcaacatc attaacgcct caagtacgcc gccgccaatc
    15001 gcccgggctt tatcagaaac gctggtgaaa tcagcggttt caccacgagg atcgatatca
    15061 ccaattttaa aaccaccaac cactgccagg ccgtcgttca acaagccacg caccatcccc
    15121 gtcaacggtg ctttaatttc atgctcacca atccaggcaa tcacatcgcc ctctttcacc
    15181 agatcgccta atttcacgtt ggatcgcata atgcctgcag caggagcacg gatcacccgt
    15241 cgggtggtat gccccataat attgccagga acatcggtat tctcctgcgc acaaccagag
    15301 taaatcacct gaccgagcca gtgcccgcga tttgtttcaa ttaccgcatg acaatccttc
    15361 cctgcagtaa agcccggccc aagcgcgatt gttactggtg ccatatctgc ccgcgttccc
    15421 aaattctgtt tcgccagaat agcgtccacc acgcaaagcg gtttcagttc atcaagcaat
    15481 gaacaggcgg gatctaccat cacagggatg aatccgcgtt cggtaagttt catcgcttcc
    15541 gcagagctgg ttgccaggcg agcagtgacg ccttcgaccg tcatttcgcc atcgaacacg
    15601 gcctgggcaa acgccacggt acaacgaatc actgtcggtt tttccacttc cagcatgatc
    15661 actttaaaac ccgcatgata cagacgtagc gccacaccac tggcgatatc tcccgcaccg
    15721 cgaatcacca ccagctggtg tccggaagga tgagaaggtt tcatcatcag cccacccggc
    15781 gcgttatttt tcacccataa tatttctgcc agcacgctga tggcgatctc ctgcggcgtt
    15841 tccgcaccta tgttgtaacc aacgggcgca tgtaaacggg caatatgttc ttcagccacg
    15901 cctttctcac gcaattgacg caggaaaagc tgaaccttgc ggcgacttgc cagcaacccc
    15961 aaccatgcaa tgggctgttc aatgagtttg tcgagggctt cacgatcctg attattcgtg
    16021 gcaatcagga caaaattatc agggcgaata tccagttctt ccactgccgc accaaacgac
    16081 tcagcatgga gaagcgtggt tgatggtggg aatagttcag gattgaggct ttcgcgataa
    16141 atatcggcaa cggcaatatc aaatcctaat agcgccgcac tctgggctat cgcccggttg
    16201 acatgccccg cgccaatcaa caccagacgt ggacgcatac catggacgct gataaatact
    16261 gacattgcac ctccacaatc tgacccgaca gcatccgcac cgttacgagc catacgtcca
    16321 tggaataatc gcggcttacg ttcctgcaat gcctgaagtg actcttcaat caccttccgc
    16381 tcaaccattc cgccaccaat tgtaccgacg atagaaccat cggcgcgcac taacatttgt
    16441 gcagaatggc ggggagtcga gcctcggcta tcaacaattt gcgccatcgc aaacggacaa
    16501 ttttgctctt cgagttttgc agcctctgtg aaaatattca taccaacctc aatggatatc
    16561 ctttcagtaa cccggaatac ccgggccgct aattcacaaa tctgcgcgcg attgcaggat
    16621 gttcttgtat atcgcccagc cagattgcct ctacgtcgtg ttgttgcagc ggttgaagga
    16681 gctcgctttg tgcaatcgca ttctcacatt gagaaaaacg gttaatgaac cagactcgcc
    16741 gacaaccttg cggtacgttt ttaaacgccc cctgagggtg gcgaaccagc gcaacgagat
    16801 cgctcagttg caaggttgca tcaggtgtta acccggtaat atcagcaaac tgcgaccagc
    16861 gatggacatt ttctgtgctc actttcgcgc ccaaagtatg tcctcccatc acagcaatca
    16921 cgcagcaact gcttttgggt atgcaaggtt cgtgctcatc aggcgctttt aacggcattc
    16981 cacgcgagcc atctgcttca atgagaatta cgtcacactc tggtcgttgc accagtgcat
    17041 caatcgcttc tggcgtaaat ccctgcactt ttccctggtt cgctttccag ctgtgaaaac
    17101 aaaatgaaat gggggatgta agagacgcat gaggaagcat ggcgggatca cgacagaaaa
    17161 ccacgggcca gtgagacgtt ggcataaaca tatgtgtcgt cgtcgtgatt aacacacgcc
    17221 tgccgctagc ctggagcagt tctgccagcc aaaaaagcaa gctggttttc cccccagcac
    17281 caacaacaga aatcacagtg ggacgtttct gcgcacctaa atcaatgact aatgccgatg
    17341 ggtcaactat acttttcacg agtctttatg acctctctgg gataaattat ccccaactta
    17401 atccatcagg aagtaacgca attatcaggc gttattagcc cctataaata atggaaccac
    17461 tatgtcagcc atcgactgta taattaccgc tgccggatta tcatcaagaa tggggcaatg
    17521 gaaaatgatg ttcccctggg aacagggaac aattcttgat acaagtatca aaaatgcgtt
    17581 gcagttttgt agccgaatta ttttagtcac cggctatcgt ggtaatgaac tgcacgaacg
    17641 ttatgcgaac cagagcaata tcactattat tcacaaccca gattatgcgc agggtttact
    17701 aacatccgtc aaggccgcag tacccgcagt gcaaacagaa cattgttttc tcacccacgg
    17761 tgacatgcca accctcacca tcgatatttt tagaaaaatc tggtcgttac gaaatgatgg
    17821 cgcaatactg ccactccata atggcatccc cggccatccg attttagtgt caaaaccatg
    17881 cctgatgcag gcaatccagc gacccaatgt caccaatatg cgtcaggcac tccttatggg
    17941 agatcattat tccgtcgaaa tagaaaatgc agaaataatt ttagatattg atactccgga
    18001 cgactttatt acagcgcaaa aaaggtatac taaaatttag aaaatgtagt taaacgattg
    18061 cgttcaaata tttaatctct ccagcactct ttatcataat gataaggaat cactgatttt
    18121 gagaaaagag aaaaagtgat aaatgggctg acaataataa ttttcaggag tgattttaat
    18181 tctcatttgg cacctgtgag ttcattcaca aaaagccttt aagtttcgag ccacctcaca
    18241 tttttttata tttcccgcca aacctggcaa gagtggtgcg attgttgctc tatcccccta
    18301 aaccaccgga tttctcaaca ccggtcactc aatgatatct gtataagcta aggagagggt
    18361 tatgggggat attatgcgtc ccattccgtt tgaggaactt ttgacgcgca tatttgatga
    18421 ataccaacaa caacgctcaa tctttggtat tcccgagcaa cagttttact cacctgtaaa
    18481 aggtaaaact gttagcgtct tcggtgaaac ctgtgccact cccgtcggcc ctgccgctgg
    18541 cccgcacacg cagctcgcgc aaaatattgt cacttcctgg ctgactggcg gacgcttcat
    18601 cgaactaaaa accgtccaaa ttcttgaccg cctggagctg gaaaagccct gtattgatgc
    18661 cgaagacgaa tgctttaaca ccgaatggtc taccgaattt accttgctta aagcctggga
    18721 tgaatacctc aaagcctggt ttgccctgca ccttctcgaa gcgatgttac agccttctga
    18781 ttccggtaaa tcgttcatct ttaatatgag catcggttac aacctcgaag gtattaagca
    18841 accgccgatg caacagttca tcgacaatat gatggacgca tctgaccatc cgaaattcgc
    18901 tcaatatcgc gatacgctga ataaattact ccaggatgac gcatttttag cccgccacgg
    18961 attgcaggaa aaacgcgaaa acttgcaagc cttacccgct cgcatcccca ccagtatggt
    19021 gcagggcgtc cccctctcca ccatgcacgg ctgtcctccg catgaaatcg aagccatttg
    19081 ccgctacatg ctggaagaaa aagggctcaa cacctttgtc aaacttaacc cgaccttact
    19141 ggggtacgcg cgtgttcgtg agatcctcga tgtctgcggt ttcggttaca taggcttaaa
    19201 agaagagtca tttgatcacg acctcaagct gacgcaagca ctggaaatgc tggagcgcct
    19261 gatggtactg gcaaaagaaa aatcactcgg ctttggcgta aaactgacta acactctcgg
    19321 caccatcaac aataaaggcg cactgcctgg tgaagagatg tatatgtcag gccgtgcgct
    19381 gttcccgctc tccatcaatg ttgcagcagt tctctctcgc gcctttgacg gcaaactgcc
    19441 catttcttat tccggtggtg ccagtcagct gactatccgc gatatttttg atacaggtat
    19501 tcgccctatt actatggcaa ccgacctgct gaaacctggc ggctatctgc gcttaagtgc
    19561 ctgcatgcgc gagctggaag gctccgacgc ctggggactt gaccatgttg acgtcgaacg
    19621 actgaacaga ctggcagcag acgcgttaac catggaatac acccagaaac actggaagcc
    19681 agaagagcgt attgaagtgg cagaagacct gccgctgacc gattgctacg ttgccccctg
    19741 tgttactgcc tgcgctatca agcaagatat tccggaatac atccgtctgc ttggcgaaca
    19801 ccgctatgcc gacgcgctgg aactcatcta ccaacgcaac gccctacctg ccattaccgg
    19861 tcatatttgc gatcaccagt gccaatacaa ctgtacccgc ctggattacg acagtgcgct
    19921 gaatatccgc gaactgaaaa aagtcgcgct ggaaaaaggt tgggatgaat ataagcaacg
    19981 ctggcacaaa cccgccggtt ctggttcacg ccatccggtt gccgtgattg gtgcaggtcc
    20041 ggcgggtctg gcagcaggtt acttccttgc cagagcgggc catccggtta cgctgtttga
    20101 acgcgaagcc aatgctggcg gcgtggtgaa aaatatcatt cctcagttcc taatgccagt
    20161 cagttaagca actgactggc tctttttcgg ggctgtgggg tatttccagg gcctctcctt
    20221 taccactctc gggaaggccc tttcccttct cgtcggtaat ttcacaagtt gtcccatact
    20281 tgcaagatcg cgcatcagct ccggtatacg tcccggtgaa gcgccctgca atgtcatcag
    20341 cattctcatc accattccgc atgattctga gaaactcagt tgattcggcc agtaaccttt
    20401 cagatgttcc gccattttaa tcatctgata tctcaccaga ttataagcca gtaagacacc
    20461 ccacagctct tgctccacaa gctccggctt tttacttctc agcgtcagcc tgctcagttg
    20521 catcgtctgt tttatctccc tgtatcccag ttcgatttcc cagcgatgac tgtacagatc
    20581 cgccatttct cctccgggga agcgcatggc gtccgtcatc gacgtcagca gatggcagac
    20641 ttttcctttg cgcgtcacgg tcagcaggcg ggctgtcact tcatttccca gtcccggcca
    20701 cttttttcgt gcctgcgggc tggttttcag cttcaccaga tgatcgcctt tacccagttt
    20761 tctgagctct tcatattgcg ctccctttct gagaggtatc atccagtggc ggtgttctcc
    20821 cgccaggctc caggcattta acagtcccag tgagtaataa cctttatcca ttaacgtcag
    20881 agtgttatcg ccggtttgtt ctataagttg ctcagcaagc tcattttcgc tgttcttcat
    20941 cgtgccgaag gctgcagccg tcagcagatg gctggtcagt tccatctggc agaccatttt
    21001 gacctgcggg tagagcgccg ggttcccggc atgtgtctgg cgggggaagg ctgcatcgtt
    21061 ctctggtgta tccggtgtgc gccagaacac accatcgatg gccagcaggg t
//
LOCUS       DAJTVJ010000073        21055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000073 DAJTVJ010000000
VERSION     DAJTVJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21055
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.073"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..210)
                     /locus_tag="OO448_002599"
     CDS             complement(<1..210)
                     /locus_tag="OO448_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR6337778.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIA"
     gene            complement(207..1768)
                     /gene="casA"
                     /locus_tag="OO448_002600"
                     /pseudo
     CDS             complement(207..1768)
                     /gene="casA"
                     /locus_tag="OO448_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(1866..4565)
                     /locus_tag="OO448_002601"
     CDS             complement(1866..4565)
                     /locus_tag="OO448_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCR6337779.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(4760..4912)
                     /locus_tag="OO448_002602"
     CDS             complement(4760..4912)
                     /locus_tag="OO448_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6337780.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(5177..5911)
                     /gene="cysH"
                     /locus_tag="OO448_002603"
     CDS             complement(5177..5911)
                     /gene="cysH"
                     /locus_tag="OO448_002603"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6337781.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(5986..7698)
                     /gene="cysI"
                     /locus_tag="OO448_002604"
     CDS             complement(5986..7698)
                     /gene="cysI"
                     /locus_tag="OO448_002604"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6337782.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(7698..9497)
                     /gene="cysJ"
                     /locus_tag="OO448_002605"
     CDS             complement(7698..9497)
                     /gene="cysJ"
                     /locus_tag="OO448_002605"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6337783.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9816..10178
                     /gene="queD"
                     /locus_tag="OO448_002606"
     CDS             9816..10178
                     /gene="queD"
                     /locus_tag="OO448_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6337784.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            10256..11527
                     /locus_tag="OO448_002607"
     CDS             10256..11527
                     /locus_tag="OO448_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6337785.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            11518..11778
                     /locus_tag="OO448_002608"
     CDS             11518..11778
                     /locus_tag="OO448_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6337786.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            11795..12370
                     /locus_tag="OO448_002609"
     CDS             11795..12370
                     /locus_tag="OO448_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6337787.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(12518..13378)
                     /locus_tag="OO448_002610"
     CDS             complement(12518..13378)
                     /locus_tag="OO448_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6337788.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(13375..14154)
                     /locus_tag="OO448_002611"
     CDS             complement(13375..14154)
                     /locus_tag="OO448_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6337789.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(14132..15541)
                     /locus_tag="OO448_002612"
     CDS             complement(14132..15541)
                     /locus_tag="OO448_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6337790.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(15563..17017)
                     /locus_tag="OO448_002613"
     CDS             complement(15563..17017)
                     /locus_tag="OO448_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6337791.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSRPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(17087..17903)
                     /locus_tag="OO448_002614"
                     /pseudo
     CDS             complement(17087..17903)
                     /locus_tag="OO448_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            18221..19498
                     /locus_tag="OO448_002615"
     CDS             18221..19498
                     /locus_tag="OO448_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6337792.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            19525..21010
                     /locus_tag="OO448_002616"
                     /pseudo
     CDS             19525..21010
                     /locus_tag="OO448_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         5055 a         5585 c         5192 g         5223 t
ORIGIN      
        1 ggctatcagc gccagtgcgg tgatacgcca ctcaggttca tcctgccact ttatttgtct
       61 gttcttcaga aacaggcgaa atccatccgt cagacaaaca tcattaaccg tcgtactacg
      121 ccgcagactg gcacgttcgc cgcgtttctc ctgcaattcc tcgtgccatt tgcgcagcgt
      181 ggctttgtgc tcctctttta caatactcat tcagcagcct cctgcttttt ctccctggcg
      241 gctttagcac tttgcttctc cgccgatgtt gtaaaatatt tcttgcgcgc ggtcatgatg
      301 cgttccaaat caacgggctc ataaggattg gtgaatacac gctcgtcaaa atcctgacgc
      361 gcgaataacc aaatttcctt attccatttg ctgagtaatt catccgcatc ctgaccttct
      421 tcaatttggc gcactaacct caggaagcga tgctgagttt tgttccagaa gtcgatatcc
      481 acaaaactaa aatcacccct tgcacctttt ggatcggaga accatgcttc tttcaatgca
      541 ctccgtaaca gacttagaat ccgtgaagcc gtttgcgcag ccagccgcag tttcggtatc
      601 tggccttctt tttattgagc agcagcggga aatgatgttc gtaccaacag cgcgctttca
      661 tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg agactgctgg
      721 cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac ttacccgttt
      781 cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg accgagtaaa
      841 actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc cacatggcat
      901 agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc tgctcaccgc
      961 aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg cgaggcattc
     1021 cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg gccagttcgc
     1081 tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg ggtagcggta
     1141 aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag ggagtttgtt
     1201 gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg ccgcgtaaac
     1261 cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac agagctaacg
     1321 cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg tctttattaa
     1381 acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga actttatctc
     1441 cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac tggaaagcat
     1501 gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct tcccagatat
     1561 catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc agcaaaaact
     1621 gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca ttttcatccg
     1681 ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg ggcaaccacg
     1741 gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga ccatcccagg
     1801 tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt cctgtcgcag
     1861 acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccaca actttcgtca
     1921 tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat gcaccacagc
     1981 ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca ccagcttcgg
     2041 cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg cttcaccaca
     2101 ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg tgtcgacagt
     2161 ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt cagcacaaac
     2221 tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc gctgcgggca
     2281 aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag ccgggctgcg
     2341 tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca gattcgccca
     2401 tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc gtcgccagga
     2461 gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc gtcctttccg
     2521 tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc tgctcgctga
     2581 atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca atcgacactc
     2641 tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct atcttcttta
     2701 ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc aaagcggcta
     2761 tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg acgatgaacc
     2821 ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg acttaccgcc
     2881 gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct gacgctacgc
     2941 tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt gacatgcgtc
     3001 agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc tgtaccccgc
     3061 gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga aagcaaaata
     3121 acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca cgacatgtaa
     3181 gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc cagaagccgc
     3241 agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt gccgacgcca
     3301 atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca tccctgactg
     3361 aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag tgattcatta
     3421 aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg ggattctggc
     3481 gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc cgttgccatt
     3541 gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct gtgcgccagg
     3601 ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa taactgcggt
     3661 ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg attctcaata
     3721 aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag aggaaagacg
     3781 gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat atccattggg
     3841 tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag cacgacggtt
     3901 gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc atcccagaat
     3961 gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc tcgtgctgca
     4021 gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg ccaaccatga
     4081 tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg cggagagagc
     4141 gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa ccagtagccc
     4201 agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat cttgagttca
     4261 gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg ccagcacagc
     4321 aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg acgaaaataa
     4381 tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc tacatccagc
     4441 gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg tcgggttttt
     4501 ccccagaaat caaaaaaagt cacaatattt ttatccttca gtaaacttaa aggatattta
     4561 cgcatatatt aaaaattatc tgtgatatat atcattcaat aaaatattta cattatgaat
     4621 aatcaaataa ttttcatccg taatatatat gaataataaa cataggcata atattcacat
     4681 aaataatata aattaaaaaa gggtgcagga gaccaaacca tcacaattca gtacgcgggg
     4741 ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca
     4801 taccacgttc tctgatactc aactcacaca acgagtcgcc taccatcagc gtgaatccca
     4861 gtactgtaaa acacagcact atgattgcca caagggcata ttttgtcagc atgtcttgcc
     4921 tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgctgg cctcaggttg
     4981 atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac
     5041 agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg ttcacgttta
     5101 aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca gaggcgttta
     5161 atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag ccaaagaaac
     5221 gtgtttcttc ttccgacatg ccaggttccc atttacgggt tgtatgggta tcaccgaccg
     5281 ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc aggtactgat
     5341 aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg
     5401 ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca gcaaaccagg
     5461 tttgcgcgtt aagttctttc agagcccggt tcatcggttc gactttgttg atgtcattgt
     5521 acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct tcctgccagg
     5581 ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc gttaactcgt
     5641 caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc accggaatat
     5701 ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag
     5761 aaagtacata ttcaccgggc agattatcca gcgcccaggc tacgcggcct tcagcgtcca
     5821 gtttttccag ttcggcgtta gtttccgcca gcgccagaat acgatctacc ttcggcagtt
     5881 cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg
     5941 cgacgctcac gcgttcttat caggcctaca agaccgggct gatggttaat cccacaaatc
     6001 gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc
     6061 ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat
     6121 ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc
     6181 gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat
     6241 cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac
     6301 accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctctgc
     6361 ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccatcgagt tttcacgctg
     6421 cggcgtgacg gcattcatta acccgctctc tttggcgatc ttctcgatct tcgctttctc
     6481 gctttccggt acaccggtga tgatcagatt ctggttcgcc gtaatgcgga aatcgccttt
     6541 gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat
     6601 gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc
     6661 aatacgatcg cctcgtccgg tgaactcata tggacggatc ggttcaaatt tgatccccgc
     6721 gcgacgctcc acttccgctt taaacgtctc aacccccacg cgctccagcg tgtatttggt
     6781 tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc
     6841 ggccaccgcc agcgtatgct ccagcggcag atagccaaac tcgctcgccg tgcgggcgta
     6901 ggttttcttg ttgccgtgtt cgatagaaag cccaccgccc accagcaggt tgaagcctac
     6961 cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca gatcgatatc
     7021 gttctgcggc gggatcacta ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag
     7081 gatcggttct tcatcagtag tggcgacttt ttcctggtcg agccagatct ccgcatacgc
     7141 gcgggtacga ggcaacagat gctcagaaat cttcttcgcc cactcgtacg cttccgcgtg
     7201 cagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagctgt
     7261 cgccagcgca tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt
     7321 cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt
     7381 gttttcaccg gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg
     7441 cagacgacag cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc
     7501 acggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttgtc
     7561 gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta
     7621 gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc
     7681 tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca
     7741 cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga
     7801 agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca
     7861 ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc ttgtacataa
     7921 actttttctt tttggtcgcg cgaccaggca agatcgatac gcgtcagcac gccctctttg
     7981 acgtagcgct gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag
     8041 aacagccagt ttttacccgg cgcttcgtcg gcggcgcgtt gctgcataaa ggcacggaac
     8101 ggcgcgatgc cggtaccggg gccaatcata atcactggcg tttgcgggtt agcgggcagg
     8161 cggaaattgt cgttatgttc gataaatacg cggacttcgc cctcttcttc cacgcgatcc
     8221 gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca
     8281 ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcgat ggaatacagg
     8341 cgcggggtca gcgggcgcag caggttaatc agcgcttcgg catccagttg cgccggagag
     8401 aaacgcacca tgtcgacaat cggcgtggtc gcggcgtaat gctgtaactt cgctttatcg
     8461 cccaccagcg gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatgttg
     8521 gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg
     8581 ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc
     8641 agtgccgggt cgttctgata ccagacaccc agcgcgtcac ccggctggta acgcaggcct
     8701 gaatcgccta agtcaatttc gatatgacga acgtcttttt cagagttacg cccggtaatt
     8761 ttctggttaa ccgaaaggct cgccaccagc ggcgcgtctt tgctgtacgg gctggtgtgg
     8821 atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat
     8881 ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacatcg
     8941 gcatcgacac ggtcgagcag gcgttcacca cccagttccg ccagcttgct gtcgaaatct
     9001 ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaacgcg
     9061 gtgttttcca gctttggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct
     9121 tccggcggtt ccccttcccc ttgcgttgac gtcactacga tgagcagttt ttcgctggcg
     9181 atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt tgctgctaat
     9241 aaatcatcgc gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga ggcggagata
     9301 atagttatac ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc agcaggctgc
     9361 tgattgagta cgccccagaa atagccagaa acccaggcaa gctgggtagg cgttaaatcg
     9421 gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt
     9481 gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt
     9541 cagcgcccat aaaacagaag agaagataag gttaacgggg tgaacggtgt ggattaaaga
     9601 cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa
     9661 caaaatagat taaccaaccc aatgaaaaac aaatgaattt agccaatcat taagataaat
     9721 cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact
     9781 ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt
     9841 tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc
     9901 tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg
     9961 gctggattat cgatttcgct gaactgaaag cggcgtttaa gccaacttat gagcgcctcg
    10021 atcaccatta tctcaatgat attccaggtc tggaaaaccc aaccagcgaa gttttagcaa
    10081 aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag
    10141 aaacctgcac cgcagggtgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat
    10201 ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga
    10261 agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg
    10321 ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa
    10381 aaatctttcc ggcgggcggt tatataccca tgcactcgcg gaactcctcc cgcaatttca
    10441 tctgaccgcg cctcttgaac gacgcatcac tcacgaaagc ctttccctgt taacgcccga
    10501 tggcgtaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc
    10561 acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg
    10621 agcgacggtg gatgcactgt atgaagaaaa cggcagagtc tgtggcgtta tttgtggtga
    10681 cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga
    10741 acgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt
    10801 gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc
    10861 agcgttgctg ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat ttctttatac
    10921 taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta cgcaaagtcg
    10981 tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc gcccgcttat
    11041 caaaaacacg gaatcactgg agtatggtgc gcatctggag ccagaaggtg gcttgcacag
    11101 tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg
    11161 cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg cacaggcggc
    11221 ggcacaaacg ctaataagcg cctgccagca ccgcgagccg caaaatctgt ttgcgcttta
    11281 tcatcacaac gtagagcgca gcctgctatg ggatgttcta caacgttatc agcatgttcc
    11341 ggcgcttttg caacgccctg gctggtatcg ggcgtggcct gcgttaatgc aggatatttc
    11401 ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tattctggcg
    11461 tcatttacgt cgtcatggcc tgtggcatct ggcgggcgat gttatcagga gtctgcgatg
    11521 tctgtagccc ataatctctg gcgcgttgct gatgcgccgc acattgttcc ggctgactcc
    11581 gttgagcgtc agatggtaca acggttgatt aacgcctgtc cggcaggtct tttttcgctc
    11641 acaccggaag gtgacttacg cgttgattat cacggctgcc tggagtgtgg cacctgccgt
    11701 ttgctgtgcg acgaatccac actacaacag tggcgctatc cgccttccgg attcggcatc
    11761 acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc tactccgcca
    11821 gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcta tcgattctga
    11881 atgccagttt atttccgtgt tgtacggcaa tatctgcacc atcagtaata tcgttaaaaa
    11941 gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa
    12001 taaagaagtc gttattcagt ttttgaaact ggtgaccgag gcggacggca ttatcagtac
    12061 caaagcctca atgttgaaag cggcaagagc ggaaggtttt ttctgtattc atcgcctgtt
    12121 tattgttgat tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga
    12181 ttgtattgag atcctgccag gcggtatgcc caaagtgctg ggctgggtga cagagaaaat
    12241 ccgccaaccg ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat
    12301 taacgcgggt gtcgtggcgc tttccaccac gaataccggg gtctggacgt tagcgaaaaa
    12361 attactttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc
    12421 acatccgaca ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtccc
    12481 caggccagaa ccgtaggcag gatcatccgg caacacgtta ttggcaatct gcgtgaatgt
    12541 ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgccaaca tctgcctgtg
    12601 aaaataccgc agcgctggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc
    12661 ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca ggtgccagca
    12721 gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc
    12781 gactgtagcc cacttcggct cccagctttt ctgccagcat agcgatttcc tggttatctg
    12841 cctctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg tcgcgagtaa
    12901 catttttcag atcctctgtg ctaaccagcc agtccggaag cgcacccggg acaatgttca
    12961 gttgttgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga cgcgccaggg
    13021 aaaggcatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg
    13081 atttgcgcac gcttacggtt ggaatatcta acgaagtcac ctggcagatg cttgcaccat
    13141 gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa
    13201 caacgtccgc aggcgttcgc tgccactgtt ccaccaacgc atccagcacc tgttccgcca
    13261 ctacaggctg aggttcaatc tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg
    13321 ccagccagct tgctattgcg gcattctcct gatttattgt cacaattgcg atattcatgg
    13381 ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg ctaccgtttg
    13441 cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat
    13501 cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg cagccattcg
    13561 ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc
    13621 aggcaagcga acccggcaac aacgcaaccc atgttcagta cgctgttcga gggtgataaa
    13681 cagcgcgtcg agagtgaaac gctccacctg ggtgaagcag ggccagccca gcatttccgc
    13741 cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag
    13801 atccagcgga ttctgatgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa
    13861 gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata
    13921 gcgcagccag tgcaatgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt
    13981 accgtttttt ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag caccgagtaa
    14041 acttcgcagt agcgaaatat ccggtccgct tttaccctga gccgccgcct gccactcttt
    14101 ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac
    14161 ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtgcc catagccagg
    14221 tcacaacaaa atcaaccaac aacactgtcg ccaggagcaa taaggtgact tgcattcccc
    14281 actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt cgactcatgg
    14341 cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa
    14401 tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg
    14461 ttaatgaact gccggaaggc aaacaggcca tcactaccag cgttgccgcc agcagcaaaa
    14521 aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg
    14581 cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct tccagaccaa
    14641 tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga
    14701 caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac aaggttttga
    14761 tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga ccaaaatagc
    14821 gatgcacgat agcgtgagct tctgcaaaac gcccctggcg cagtagccag cgtggtgatt
    14881 ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg cagagctgcc gatgccagta
    14941 gccagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata cttgccagca
    15001 catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga tggcggcgcg
    15061 gggaaaattc agccagcaag gtgtgaccta ctgaataatc gcctcccaga ccaatgccaa
    15121 tcaaaatgcg cagtccaata agatgctctg gcgtggtggc aaaaaattgt aaaaacgaag
    15181 caagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata tggtcggaga
    15241 tccacccaag aaccaggctg ccaaggaaca aaccaaggag cgccgagccg ccgatcattc
    15301 ccgccataaa cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg taaccaatga
    15361 cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca atgcggcagt
    15421 gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt
    15481 cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgccccgtca
    15541 tcgcggaacg ctgcctgaca cattattttt caatcggata gatagtaccc gtattcataa
    15601 tgccattagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt
    15661 ccagtttgct ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc atcgaaccgc
    15721 cgaggcgaat ggtttcttca cagatgatct tgttgagcgg attgtggtac ttgtcgattt
    15781 cctcttccgg cttacagtca acgacgttgt aatcgtagac gaagtacatg ttggtgccgt
    15841 tctgatagct atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact
    15901 cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc cagcagccgg
    15961 acacttcagt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca
    16021 ctttatccgg tccccagttc aggttgttaa accaggtttc gatcagcttg ctgtccacgc
    16081 gctggcattg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc gccttcgcaa
    16141 tgcgagggtt accttcagcc ataaagatca gcacgcattt tccgtctgca aaatgggtga
    16201 agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga tacccttcca
    16261 ccatgacttc acgcaggatg ttgaagtcgg ttttcatgtc ttccaggata tagccgtaga
    16321 agaggttgtt ttccggggtg aatttaaaga ttttcactgt tacttcagtg atatagcaaa
    16381 gtgcaccttc gttaccgatg atgatgtggc gaatgtccgg gccagccgcg cggcgtggca
    16441 cgtttttaat gcgtgtgacg gtgccatcag ccaatactgc ttccagacca acgaccatat
    16501 cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct accaagccgc
    16561 ccatctgcgc cagcggcctt gactgcggag aatgccccgt ggtgtaacct ttttcacgca
    16621 acgcattttc cagcacctcc agcggaacgc cacattgcgc cgtcgcctgc atattctcaa
    16681 tatcaatatt aatgatttga ttcatggcgg agccgtcaag caccaccgag ttttccacaa
    16741 cggtttccag cccaccttca gtggcggaag cgccggtacg cggcacaccg ttaattttgt
    16801 gcgcattcat aaaattcagc acacgggata cttgctctgt ggaaccgagt tttacgaccg
    16861 ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga aaacggtcaa
    16921 tactgttctt ctttaatact gtttcatcgg taattacgcg atctgcacca acaatttcct
    16981 ttagttggtc gacaatcgct gcgcgagata aagacatagt aattccttcc tgataataaa
    17041 aatggaataa tcattaataa gagaaaacag aaaccaccgt tatggattag cgcactaaat
    17101 aaccaccatt gaccactaat aaatgcccat tgacataatt tgaagccgga cttgcgagga
    17161 atacagctgc gcccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa
    17221 gaacgcgctg attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat
    17281 agccaggagc aataccattt acctgaatat tatattgacc tagttcatca caataagctt
    17341 tggtgaatcc ggcaagagca tgtttagtgg cagaatatgc aggtgaccat tgtccaccta
    17401 agtaagagaa caatgaacag atattaataa ttttgccgct tttctgcggg atcataattt
    17461 ttgcagcttc atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc
    17521 agtcggcacg accgaagtcc agcaccttat tcagcttaca aataccggca ttgtttacca
    17581 gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt
    17641 ctgcggtgat atccacctgc atgaagtcca cctcaacacc ctgtttttca atcatttcct
    17701 ttgtttcgcc gttatctttc aatcatttcc tttgtttcgc cgttatcttt gacgaaacta
    17761 ggaataaaga tatttgcgcc agctttggcc aacgccatgg caaatgcctg gcctaaaccg
    17821 ctattcccac cggtaacaat tgcggtttta cctttcagtg agaaaaaatc cattgagaac
    17881 gcattgagag attcgattga catagttagc tccaatttcc gtaaggcaaa aaaaagaaag
    17941 gtaacttccc ccgcgagagg gaagttacct ttctcattat ctctggtaac tggagataat
    18001 ttttagcata acggcataac tgctaatgtg atcgtaatca cagtgtgata ttcgtattgt
    18061 caaatagttt taaaaagcag aagcgtgatt actctcacac aacacctctg ctttccatgt
    18121 tatttcttat tgcaattact agagacctga gaaaagtaat ccacctgaaa gggcggctta
    18181 cttttctctt ttttttgtct attacaaatg ggaatacgat atgcaacaca actcatatcg
    18241 ccgttggata accctcgcga taattagttt tagcggcggc gttagttttg acctggctta
    18301 tttacgttat atttttcaaa ttcccatggc gaaatttatg ggattcagca ataccgagat
    18361 aggtttaata atgagtacct ttggtattgc ggctattatt ctttatgccc ccagcggcgt
    18421 tattgccgat aaattttcac accgcaaaat gattacttcc gcgatgatca ttaccggatt
    18481 actgggtctg ttaatggcaa cgtatccacc gctgtgggta atgctctgta ttcaggtcgc
    18541 ctttgcgata acgacgattt taatgctgtg gtcggtgtcg attaaagccg cctcgttgct
    18601 gggcgatcat agtgagcaag ggaaaattat gggctggatg gaagggctgc gcggcgtcgg
    18661 tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac cggatgacag
    18721 cgccagcctg aaaacagtca ttatcatcta cagtgtggtt tacatcttgt tggggattct
    18781 gtgctggttt tttgttagcg ataacaacaa cctgcgcagt gccaataacg aagaaaaaca
    18841 gtcattccag cttagcgaca tccttgccgt tttgcgtatc agcaccacct ggtattgcag
    18901 catggtgatt tttggtgtct tcacaatcta cgccattctg agttactcca ccaactatct
    18961 gaccgaaatg tacggcatgt cgctggtggc agcgagctac atggggattg tgatcaacaa
    19021 aatcttccgc gcgctgtgcg gcccacttgg aggcattatc accacctaca gtaaagtgaa
    19081 atcccctacc cgcgtgattc aaatcctttc catcatcggt ttgctggcgt tgacagccct
    19141 gctcgtcacg aactctaacc cccaatcggt cgcgatgggg attggcctga ttttactgct
    19201 gggattcacc tgttacgcct cacgcgggct gtactgggcc tgccccggcg aagcgagaac
    19261 accgtcttac attatgggca ccacggtagg tatttgttcg gtgattggat tcctgccgga
    19321 tgtcttcgtt tacccgatta tcggccactg gcaagacacc ctgcccgctg cagaagccta
    19381 ccgcaatatg tggctgatgg gcatggcggc gctcggcatg gtgattgtct ttaccttttt
    19441 gctgttccaa aaaattcgta ctgctgatag cgcccccgca atagctagca gcaagtaagc
    19501 caggaaaatt acaggagaaa aatcatgtcg aaaaaataca tcatagggat tgatggcgga
    19561 agtcagagca caaaagtggt gatgtacgat ctggaaggta atgtggtttg cgaaggtaaa
    19621 ggcttattac agccgatgca cacgcctgat gccgatactg cagaacatcc tgacgacgat
    19681 ttatgggcat cattatgttt tgcaggtcac gatttgatga gccagtttgc cgggaataaa
    19741 gaagatattg ttggcatcgg cctgggctcc atccgttgct gccgtgcgtt attgaaagcc
    19801 gacggcacgc cagctgcgcc gttgattagc tggcaggatg cacgcgttac acgcccttac
    19861 gaacatacga atcctgacgt ggcgtatgta acctcttttt cgggttatct gacgcatcgc
    19921 ttaaccggcg aatttaaaga taatatcgcc aactattttg gtcagtggcc ggtggattat
    19981 aagacctggg catggagcga agatgccgca gtcatggaga agtttaatat cccccgtcat
    20041 atgctgtttg atgtgcaaat gccaggcact gttcttgggc atatcacacc tcaagccgcg
    20101 ctggcgacgc atttcccggc aggactgccg gttgtttgta ccaccagtga taaaccggta
    20161 gaagctctgg gagccggatt actggatgat gaaactgcgg taatttctct tggcacttac
    20221 atcgcattga tgatgaacgg caaagccttg ccgaaagatc cggtggcgta ctggccgatt
    20281 atgtcttcta ttccgcaaac attgctgtat gaaggttacg gtattcgcaa aggcatgtgg
    20341 acggtgagct ggctgcgcga catgttaggc gagtcgttaa ttcaggatgc cagggcgcag
    20401 gatctttcac cggaagattt actcaacaag aaagcctcta gtgtgccacc tggctgtaat
    20461 gggctgatga cggtgctgga ctggctgacc aatccgtggg aaccgtacaa acgcgggatt
    20521 atgatcggct ttgattccag catggattac gcatggatat atcgttcgat attggaaagc
    20581 gtggcgctga cgctgaagaa caattacgac aatatgtgta atgaaatgaa tcactttgcg
    20641 aaacatgtaa tcattactgg cggcggttcg aacaggatct gtttatgcag atttttgccg
    20701 acgtgttcaa ccttccggca cgccgtaacg ccattaacgg ttgtgcaagc ctgggggcag
    20761 cgattaatac aggggtaggt ctggggctat acccggatta cgcaacggct gtcgataaaa
    20821 tggttcgcgt gaaagatatc tttataccga ttgagagcaa tgccaaacgc tacgacgcga
    20881 tgaataaagg cattttcaaa gacctaacca aacatactga tgtgatcctg aaaaaatcgt
    20941 atgaagtgat gcatggggaa ttggggaatt ggggaatgcg gattcgatcc agagctggtc
    21001 gaatgcgtga ataaggtagg ccgggcggaa aactgcccgg cctgaacata cctga
//
LOCUS       DAJTVJ010000074        20993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000074 DAJTVJ010000000
VERSION     DAJTVJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20993
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.074"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..160)
                     /locus_tag="OO448_002617"
     CDS             complement(<1..160)
                     /locus_tag="OO448_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337793.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFG"
     gene            332..1552
                     /gene="coaBC"
                     /locus_tag="OO448_002618"
     CDS             332..1552
                     /gene="coaBC"
                     /locus_tag="OO448_002618"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6337794.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1530..1988
                     /gene="dut"
                     /locus_tag="OO448_002619"
     CDS             1530..1988
                     /gene="dut"
                     /locus_tag="OO448_002619"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6337795.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2095..2691
                     /gene="slmA"
                     /locus_tag="OO448_002620"
     CDS             2095..2691
                     /gene="slmA"
                     /locus_tag="OO448_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6337796.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2728..3369)
                     /gene="pyrE"
                     /locus_tag="OO448_002621"
     CDS             complement(2728..3369)
                     /gene="pyrE"
                     /locus_tag="OO448_002621"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6337797.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3435..4151)
                     /gene="rph"
                     /locus_tag="OO448_002622"
     CDS             complement(3435..4151)
                     /gene="rph"
                     /locus_tag="OO448_002622"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6337798.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4278..5141
                     /locus_tag="OO448_002623"
     CDS             4278..5141
                     /locus_tag="OO448_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6337799.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5363..6187
                     /gene="dinD"
                     /locus_tag="OO448_002624"
     CDS             5363..6187
                     /gene="dinD"
                     /locus_tag="OO448_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6337800.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKGQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6479..7096
                     /locus_tag="OO448_002625"
     CDS             6479..7096
                     /locus_tag="OO448_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6337801.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7093..8775)
                     /gene="ligB"
                     /locus_tag="OO448_002626"
     CDS             complement(7093..8775)
                     /gene="ligB"
                     /locus_tag="OO448_002626"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6337802.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9033..9656
                     /gene="gmk"
                     /locus_tag="OO448_002627"
     CDS             9033..9656
                     /gene="gmk"
                     /locus_tag="OO448_002627"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6337803.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9711..9986
                     /gene="rpoZ"
                     /locus_tag="OO448_002628"
     CDS             9711..9986
                     /gene="rpoZ"
                     /locus_tag="OO448_002628"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6337804.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10005..12113
                     /gene="spoT"
                     /locus_tag="OO448_002629"
     CDS             10005..12113
                     /gene="spoT"
                     /locus_tag="OO448_002629"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6337805.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12120..12809
                     /gene="trmH"
                     /locus_tag="OO448_002630"
     CDS             12120..12809
                     /gene="trmH"
                     /locus_tag="OO448_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6337806.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12815..14896
                     /gene="recG"
                     /locus_tag="OO448_002631"
     CDS             12815..14896
                     /gene="recG"
                     /locus_tag="OO448_002631"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6337807.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15065..16270)
                     /gene="gltS"
                     /locus_tag="OO448_002632"
     CDS             complement(15065..16270)
                     /gene="gltS"
                     /locus_tag="OO448_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6337808.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16550..17941
                     /gene="xanP"
                     /locus_tag="OO448_002633"
     CDS             16550..17941
                     /gene="xanP"
                     /locus_tag="OO448_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6337809.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18062..19771
                     /locus_tag="OO448_002634"
     CDS             18062..19771
                     /locus_tag="OO448_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6337810.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19823..>20993)
                     /gene="yicI"
                     /locus_tag="OO448_002635"
     CDS             complement(19823..>20993)
                     /gene="yicI"
                     /locus_tag="OO448_002635"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6337811.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5076 a         5711 c         5385 g         4821 t
ORIGIN      
        1 agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg cgcgttccgg
       61 tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca aacttcagca
      121 ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc tttgtggtgc
      181 tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc tggaaagcgc
      241 ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata aaatcgccaa
      301 cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa aaatcgttct
      361 cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc gtttgcgcga
      421 tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta tcaccccact
      481 tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc cggcagccga
      541 agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc tcgcccctgc
      601 cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg tatcgacgat
      661 ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc agatgtaccg
      721 tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc tcatctgggg
      781 gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc tcgatccgtt
      841 aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga aacatctgaa
      901 cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt atatctctaa
      961 tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc gtggcgcgaa
     1021 cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta aacgtgttga
     1081 tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc agcaaaatat
     1141 ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc cagagaaaat
     1201 caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa accccgatat
     1261 cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat ttgccgccga
     1321 aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc ttgatctgat
     1381 ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca acgcattaca
     1441 ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc tccttggcca
     1501 attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt aagattctgg
     1561 acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc tctgccggac
     1621 ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac tctacgctgg
     1681 ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg atgctgccgc
     1741 gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga ttgatcgatt
     1801 ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac agcttcacca
     1861 ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag gctgaattta
     1921 atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt cactctggtc
     1981 gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat ttcgtttgcg
     2041 gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg taacatggca
     2101 gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct ggcgctgatg
     2161 ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc ctctgtcggc
     2221 gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt cgatagtctg
     2281 attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa agatgagaaa
     2341 gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg tgagcgtaat
     2401 cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga tcgcctgcaa
     2461 gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt attgcgtgaa
     2521 aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc aagccagatc
     2581 ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa ataccgcccg
     2641 acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta atatgacgcc
     2701 ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat aggccttaac
     2761 cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca ggtctttcag
     2821 ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa tcgccgaaat
     2881 ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa gcgtcgcgcc
     2941 attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga tcacatcatc
     3001 taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt caccgtggtt
     3061 ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct ccgccagcgc
     3121 cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca gcagatcgaa
     3181 ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg ccaggtcgcg
     3241 cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg atttcagcgt
     3301 aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact ggcgctgata
     3361 tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag gcgactcatc
     3421 agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat ggattcgatt
     3481 ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg ttcgccttct
     3541 gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt catgtcggtc
     3601 tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt cacaatcccg
     3661 acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc gttttccacc
     3721 agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc ggtacgagtg
     3781 ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc cagcgctttc
     3841 aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat tgtgcgtcca
     3901 ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg cggcagcata
     3961 ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg cacgccttct
     4021 tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac cgagccttct
     4081 gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt gctacggcct
     4141 gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt atacggactt
     4201 ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca cctctccttt
     4261 tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt gaaatcaagg
     4321 gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat ctggaaactt
     4381 actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag cgtattcgtt
     4441 ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat gttagcgcgc
     4501 aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc gcgaactggg
     4561 taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc tggccgggcg
     4621 taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc gcggcgttgg
     4681 atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg ctgaaagcgt
     4741 tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc gcccatatgc
     4801 cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat gctcaagtgc
     4861 aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga attgatgttg
     4921 ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt ctgaagaaaa
     4981 aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc gagtcgaaca
     5041 ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag ctgaaagtgt
     5101 tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca ctaattctta
     5161 cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct tccccgcaac
     5221 attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc gcagcgcagt
     5281 gagaacgtga gatactcaca actgtatata aatacagtta tagacttact ttctttgcaa
     5341 tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag aaatacggca
     5401 ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt tactggatta
     5461 tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt gcgaagccag
     5521 caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat taggttcagg
     5581 ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc tggtagtaca
     5641 aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg ctatccagac
     5701 ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag acgaaaagcg
     5761 tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg cagcgcagca
     5821 agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt accgggggct
     5881 gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa agggtcagaa
     5941 gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag ctacccaaac
     6001 agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata ccacacactt
     6061 tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca tgcctgagga
     6121 gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa ttacagagaa
     6181 gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg taagccaaag
     6241 tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc cctcaatttt
     6301 aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca tctacactac
     6361 agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt aaaaaatctc
     6421 aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg agagaaccat
     6481 gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg gggcgctggc
     6541 ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg ccaccgcaat
     6601 tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt gggtcaaaca
     6661 cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg ttgctcctgt
     6721 aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg tggtcttttc
     6781 tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg ccgttgtcgc
     6841 ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta aacgcatccc
     6901 actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg tgctgtacat
     6961 cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc tggtattcgg
     7021 cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct tttactacag
     7081 ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc aactgcccag
     7141 cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc cagatccagt
     7201 ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc aggaccgttc
     7261 atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca cccagcgagt
     7321 aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt tacttttcgc
     7381 gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa agatatgctc
     7441 aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat ccagcccaag
     7501 aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac aaacatcaga
     7561 agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt ttgtacgttc
     7621 tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa ggctgacgag
     7681 gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac caatattcac
     7741 ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca ccgatatttt
     7801 accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag ctacaggttg
     7861 atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg attcgggttc
     7921 cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt tccaccactc
     7981 attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc tctgcgttag
     8041 agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg gtccatccgg
     8101 ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct ggcgcatcat
     8161 caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga tatgcccctc
     8221 gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc taacggtttg
     8281 cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca ggccattacc
     8341 gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg caacgccatc
     8401 aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta atgcgttttt
     8461 atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac catttaaagg
     8521 cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg taagacgggc
     8581 gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc agtagtcatc
     8641 gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc tggctggcga
     8701 ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta ttaatatcgc
     8761 catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac aacgtgatga
     8821 aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa tcgtctttat
     8881 tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac tttgcgcaaa
     8941 aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc gtatgtaggc
     9001 tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt tatattgttt
     9061 ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa acccaaccgt
     9121 tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct ggtgaagtcc
     9181 acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt agcagagatg
     9241 cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt gaggccattg
     9301 agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag ggcgcgcagc
     9361 aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg tccaaaattg
     9421 aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt gcaaagcgta
     9481 tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg attgtgaatg
     9541 atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa cgtctgcgca
     9601 tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca gactgaacct
     9661 gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt atggcacgcg
     9721 taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta ctggtcgccg
     9781 cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg gaagaaaacg
     9841 ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac aaccagatcc
     9901 tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta caagccgtta
     9961 ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat ctgtttgaaa
    10021 gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt ctgcggcagg
    10081 cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt gaaccctaca
    10141 tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac tatgaaacgc
    10201 tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac caggatatgg
    10261 aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa cttgataaac
    10321 tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg attatggcga
    10381 tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac aacatgcgca
    10441 cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact ctcgaaattt
    10501 acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc gaagagctgg
    10561 gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg aaagccgcgc
    10621 gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg cgtttgcagg
    10681 aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg atttactgca
    10741 aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct ttccgcgtga
    10801 tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc ctgtacaagc
    10861 cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc tatcagtctt
    10921 tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc cgtactgaag
    10981 atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa gagcacggcg
    11041 aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcttg ctggagctgc
    11101 aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat ctcttcccgg
    11161 atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc ggtgcaacgc
    11221 ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg ggcgcacgcg
    11281 ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc gttgaaatca
    11341 ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt agctcaaaag
    11401 cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct gtaagcctgg
    11461 gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa atcccgcagg
    11521 aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat ctgctggcag
    11581 aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa catggggacg
    11641 cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt gccgatggcg
    11701 tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt atcgcccacg
    11761 tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc cgtggctacc
    11821 agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg caggagttca
    11881 tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac ctgacggcgg
    11941 caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa gatggtcgcg
    12001 tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg aatatcatgc
    12061 gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat taatgtttta
    12121 tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag cctgatctga
    12181 ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt cgtaccgcag
    12241 atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg cgcaccatgg
    12301 cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc accattggcg
    12361 atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat ctttctgata
    12421 acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg atgggacagg
    12481 agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc atcattccga
    12541 tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt ctttacgaag
    12601 cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg ttgccggaag
    12661 cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa gtcgcaaaac
    12721 gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct gatgccgact
    12781 ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt tagatgctgt
    12841 cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg cgaaaatcaa
    12901 cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag atcgcaccca
    12961 tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag gcgaagtgct
    13021 gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca gcgacggttc
    13081 cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata gcctggcgac
    13141 gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg cggagatgat
    13201 ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg aaacgctcac
    13261 gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat taaccgacca
    13321 ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg aactgtcaca
    13381 aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac cgacgctaca
    13441 gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc tggaagaact
    13501 gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt ttcatgccca
    13561 gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac cgttcaagcc
    13621 aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc tggatgtgcc
    13681 gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg ccgccctcgc
    13741 cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa ccgaattact
    13801 cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta tcgaagtggg
    13861 ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag ccatcgccag
    13921 cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg tgcagtttaa
    13981 cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc agcgtctggc
    14041 gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca tgaccgccac
    14101 gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct cggtgataga
    14161 tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata ctcgccgtac
    14221 cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg cgtactgggt
    14281 ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag ctacctggga
    14341 agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc ggatgaaacc
    14401 agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc tgctggttgc
    14461 cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga ttatcgaaaa
    14521 ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag gtcgtggcgc
    14581 ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg cgcaaattcg
    14641 cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag atctggagat
    14701 tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat ttaaagtggc
    14761 ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac gccatattca
    14821 cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg agacggaacg
    14881 ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta ccctaccttc
    14941 ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat acgcaggctt
    15001 gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac cgccgcttca
    15061 tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc gcattgacga
    15121 tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc gacgggccaa
    15181 agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg aggccaaaac
    15241 cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg cgccaggtaa
    15301 cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc agcatcggca
    15361 gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc aggaacaacg
    15421 acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa aagcccatta
    15481 ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag gtcggcagtt
    15541 caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg cagatagcaa
    15601 tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca tccggctttt
    15661 caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg gagtgtttca
    15721 ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg aacgttgcac
    15781 aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac aatttactcc
    15841 acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac agccccatca
    15901 gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc atcaccaaca
    15961 gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc aaactggcaa
    16021 tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg cgcagggaca
    16081 tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc agcgccacca
    16141 acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag acggaatgga
    16201 ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct aaagtatcga
    16261 gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc atacttcatc
    16321 gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct gggtcaaatt
    16381 ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa aagttatata
    16441 aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg tttgcttttc
    16501 tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga tgtctgtttc
    16561 caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg aactgattta
    16621 ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc agcatctgct
    16681 ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc tgggtttacc
    16741 ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg tggcatcgat
    16801 tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc agggcaccag
    16861 cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg gtggtgctga
    16921 tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt gcaccgagat
    16981 ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg tttctggcgt
    17041 tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca ttggcggcgg
    17101 ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc tggcaggcgt
    17161 ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac gcgtggcctc
    17221 actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca tgttgccaga
    17281 aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct attacggtct
    17341 tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca cttcgctgga
    17401 aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt ccggtccgct
    17461 gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt ttgtttcggc
    17521 ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga tccagttgac
    17581 cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg tgctgggtct
    17641 gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg gcggcgcaac
    17701 gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc gtgagccgct
    17761 gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg gcgtgtctca
    17821 gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct cctccgggat
    17881 cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag aaaaacagta
    17941 attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac cattcccgcc
    18001 ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg gatggaagac
    18061 catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag tggcgatcgc
    18121 tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg catgggtttc
    18181 cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt ccgcgccatc
    18241 tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga ccctggtggc
    18301 caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc atgtcgatac
    18361 catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc taccgttcaa
    18421 agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg aatggaaact
    18481 gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg gtaaagtgct
    18541 gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg atgttcctgc
    18601 caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga ctaacctggg
    18661 tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg acggcagctg
    18721 gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat cgctaaccga
    18781 cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg aagtgatcga
    18841 tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct tgcgcaacat
    18901 gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga acgctagcga
    18961 gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat tttctccgca
    19021 gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca gaacgtcagg
    19081 gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg ccggactgga
    19141 atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg gttggttaaa
    19201 cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata tcgaccctga
    19261 cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc tggttaccga
    19321 tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg cgacgtttaa
    19381 tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca cggtgaatat
    19441 cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca gtgtttcaca
    19501 aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg tgaatatttt
    19561 gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc agcttaccgc
    19621 cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc aattgcatgc
    19681 cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg tttccagcgg
    19741 tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta cgactcagac
    19801 gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct tgaggcgtaa
    19861 ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt actttcacaa
    19921 tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca gtaatcgtgt
    19981 taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg ggcacttcac
    20041 atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct tcgtgccacg
    20101 cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca cgcacataaa
    20161 cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg agctcattgt
    20221 tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg cccgcctcag
    20281 tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca aggtaatcac
    20341 aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta ccccgcgctt
    20401 tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt tgcgtgaaga
    20461 agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa gatttgctgc
    20521 catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg taaacgtgtg
    20581 ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa cctgaaaggc
    20641 caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg taacaatcgc
    20701 caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca aacaagacag
    20761 cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg tagatgtacg
    20821 cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca tcagttggga
    20881 tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga cctttcagtt
    20941 tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc aga
//
LOCUS       DAJTVJ010000075        20714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000075 DAJTVJ010000000
VERSION     DAJTVJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.075"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO448_002636"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO448_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6337812.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO448_002637"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO448_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6337813.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO448_002638"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO448_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6337814.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO448_002639"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO448_002639"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6337815.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO448_002640"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO448_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6337816.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO448_002641"
     CDS             complement(8217..9110)
                     /locus_tag="OO448_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6337817.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO448_002642"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO448_002642"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6337818.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO448_002643"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO448_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6337819.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO448_002644"
     CDS             complement(10241..11473)
                     /locus_tag="OO448_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6337820.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO448_002645"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO448_002645"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6337821.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO448_002646"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO448_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6337822.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16542)
                     /locus_tag="OO448_002647"
     CDS             complement(14278..16542)
                     /locus_tag="OO448_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCR6337823.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16749..17033)
                     /gene="ihfB"
                     /locus_tag="OO448_002648"
     CDS             complement(16749..17033)
                     /gene="ihfB"
                     /locus_tag="OO448_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6337824.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17193..18866)
                     /gene="rpsA"
                     /locus_tag="OO448_002649"
     CDS             complement(17193..18866)
                     /gene="rpsA"
                     /locus_tag="OO448_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6337825.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18977..19660)
                     /gene="cmk"
                     /locus_tag="OO448_002650"
     CDS             complement(18977..19660)
                     /gene="cmk"
                     /locus_tag="OO448_002650"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6337826.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19833..20597)
                     /gene="ycaL"
                     /locus_tag="OO448_002651"
     CDS             complement(19833..20597)
                     /gene="ycaL"
                     /locus_tag="OO448_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6337827.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4970 a         5733 c         5046 g         4965 t
ORIGIN      
        1 cgtaatgtgc cactttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tctcctgaga aggcgcttcg tcacttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acgtgttcgg tattctggaa
      181 gactttacgc accagtttat aggtggtgcc tttctccggg ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccagc catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 cagcgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tcagacgtac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgttcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttcg cgggaggtta actcctcggt cagtcggcta agttcgattt ccatctgctc
      901 gatggcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgttccgga cgttttggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacccaatgc
     1081 cttatccgac atagagcgca aatcatcagc ggagagataa gccagctcgc gacggtgtaa
     1141 gcgccgttca acgacgttat ctttcaccat gcgcatcact gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 cagattgtcc atctccgctt cgcagaaagt cagtgctttt tccagctggt tgcggcggga
     1321 ccggttatta ctcagttgcg catgcagctc gtcacggcga attcgcgccc gctcttctgc
     1381 cccgctatcg gcgcgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccacgcaacg cttcacgagc gcgagtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agcttttcgt tgagatcgct attaccgctc agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgcct gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt agctgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga cgcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gacgatcggc gattttcacc actaccgctt tttccagctc
     2281 atcaacgctg aacacgctgt catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcagttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagcgc tgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacgcc gccaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cagcgttttt
     2581 gcgcgcgccc acttcatcgc gttcaacaat cgcctctcgc tcacgctcca gcaactgttg
     2641 cagatattcg gtgacgtcct ggctggaggt aaactcttca ccgcactgct cgctcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggcgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctggcgcag tgccatgcgc tcttcacggg cgttagacac
     2821 gctatcggaa agagaggcaa tgcgcgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaagtctgcc agcaggcgtt cggcttcttg
     2941 ctgctcgcgc agacgctgtt ccagttcact taagcgcatc cgcaacggtt gaacctgctc
     3001 tgccaggtga cgctgatcga ccccttcgcg cagcagttcg cgggcgacat cccaagcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 ctgctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cgcgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcgttctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcgttttgct cttccagacg gatctgcagc tcatcgagat ccgcttcgta
     3481 gcgttcgatc ttctcctgct gacgctgtgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtaactcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatggt ggtggattta cccgccccgt taccgccaga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgattctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcgaaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagttttgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccga catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccccagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacacgccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaatga cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccaccg cccgcatcca gcacacgcag tttttgcgga
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgcgaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gaaggtattc
     7801 gcttatggcg cgaaaatccg ggatcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtgccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gagttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggcg
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccaag ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccccttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagtga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt tgtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcaa cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca acgccgaggt
    12721 agcttcgtcc agaatcagaa tcgggctatc acgcaacaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ctatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcgaccg ttttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggcacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat tagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcgctggtg gtcacctgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgataca acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgattgacg caccttcacg
    13801 cacaaccgtt atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaaaaat gaaaccggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agcccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttaaaaggcg caatggttgg ccacagtcgg cggaacgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctccct
    14341 gaggctgcta atccgccagc cttgcgctga aaaattgacc gctatttgcc cctgatgagg
    14401 agtatcaagc cacttataac cctgttgttg atagcgatgt ttaactttgc ttgagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgtctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagcg gggacttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccttt
    14701 atcgttgcta gcttgtaaag gccagtgcac gctgaaacgc aatccttgcc attgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatgtg tgcaatattg agtccagccc tccccggtga tccagatgtt catgactcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcgggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac acctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctaca gccacacaca ttgctggcaa
    15121 cgttcgccag gcgtttaatc gccataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacgt tcagcaatgt tgatccaccc ttctggcaaa ctctttaacc cccagaaaag
    15241 taaagccaaa gaccggtcgg caagaaacca taacccttgc tccaggatta acggcccgct
    15301 taaatgcaca accatcgcgg cgaggatcaa cggaaccgtg ataaatgtca ccaagggaat
    15361 tgctaataga tttgcaataa acgaggtcag actaatgcca tgaaatatga cgatttgcac
    15421 gggcataagc agaagtgtga tacccagttg cagatggatg agggaaacaa ctgcacgcaa
    15481 taccggcggc agttgccact caggacaggg aaaccactga taccaaaaaa tcagtgccgc
    15541 gaccgcagcg gcagagagcc ataaactttg cgagagaatg gcaacaggat ccatcagcaa
    15601 aattgccgcc agacaacata tccatacatc ccagccactc cactgtcgcc cacttaactt
    15661 aagcattccc cacgtagcaa gcgccaccac ggtacgcaat gcaggaggtt gcattcccgt
    15721 caaccaggca taaaaagcag cacagcagat cccaccaatt aatggtattt gccagtggat
    15781 ccagcgccca ggcagaaaaa tttgtccact gcgaatgagt ccggcagcca gcaacaccgc
    15841 aaaagcgatg tgcaatcccg atatcgccat taaatgcgcc gttccagtat cgcgcatgat
    15901 atttttgatt tctttgggga cggataaccg ttcccccata cctaaaccaa gaataaccgc
    15961 attccacgga tagggttgca gcgttgtttg tagtgacgcc agatactgtg cacgcaggct
    16021 acaattcggt tcaataagac ttgcctgcag aaagcggccg gtgagcggct gatgctgggc
    16081 aatggcataa cgctggctat caaagccgcc atcattaagt tgaccatgaa cggcacgaac
    16141 tttgagtttc attgaccaca gttgtccggc acaaaccgct tgcggaagat attcgccata
    16201 cagcacaagg cctggcgcag ggaatatacg tcgaccttgt agataagtaa tttgaccgta
    16261 atgagtggtc atgccgtcag tagcagtgat ctcaacaatt gcatcctgcg tcgcgccagt
    16321 taaggtttct cctgcccaca aaatttgctt tgctgataat atgccccaaa cgaaaaacag
    16381 caacgtcagc gcgatataac ggacggtttt aacaggtata aatgccagta cgcaggcgaa
    16441 gagagtcaga aacgcaaggg ctaatgtccc aggcaattgg ggcaaaatca gcaacggaaa
    16501 aattccgcaa attatgcata cgtcgactgt cgttattttc atgcactcct ccctgtttat
    16561 ggaaagtgtg caactttgtt gagcggttgt ctggtgggaa ttttgcgttc cccgaggcac
    16621 attcagaaga atttaacgag agggtgaaaa ctgctcaaaa ctctgcgagg cagcttccag
    16681 ttcctgatta gagaaatgaa aaaagcaccc gacaggtgct tttctcgcgt tcaagtttga
    16741 gtaaaaactt aaccgtaaat attggcgcga tcgcgcagtt ctttaccagg tttaaagtga
    16801 ggaacgtatt ttccttccag ttctacttta tcgccagtct tcggattacg tccggtacgt
    16861 ggtgcgcggt agtgcaaaga gaaactgccg aaaccgcgga tttcaatacg ctcgccctgc
    16921 gcaagagtcg aggccatatg ctccagcatc tcttttactg catcttcaac cgtcttggcg
    16981 ggaatgtgcg attgctgggt ggcaagtctt tctatcaatt ctgacttggt catgattcct
    17041 ccggttcctt aaaggcaaat taagcggctg ctgttgctgc attgattaag ggcggccgta
    17101 gccgccctta gtgcttgatt acaggacgaa acctgcaatc tgtcaagtaa actcaacaaa
    17161 cttcggaata aaaatcccga agagtcagag aattactcgc ctttagctgc tttgaaagct
    17221 tcagccattg cgttgttgga gaagtttgca tcttcctgtt tgttaacagt tgcgattgca
    17281 tctttctcgt cagcttcgtc tttcgcacga acagacaggc tgattgcgcg gtttttacga
    17341 tcaacgccgg tgaatttagc ttcaacttcg tcgccaacgc tcagaaccaa ggtagcgtct
    17401 tcaacgcggt cacgagatgc ttcagaagca cgcaggtaac cttcaacgcc gtcagccagt
    17461 tctacggttg cgcctttagc gtcaactgca gttactttac cggttacgat agcgcctttc
    17521 ttgttcagag caacccagtt gttgaacgga tcttctgcga gctgtttaac gcccagggag
    17581 atacgttcac gttctgcgtc aacctgcaga acaactgcag cgatttcgtc gccttttttg
    17641 tattcacgaa ctgcttcttc gcctgcaacg ttccaggaga tgtcagacag gtgaaccagg
    17701 ccgtcgatgc cgccgtccag gccgatgaag ataccgaagt cagtgataga cttgatttta
    17761 ccttcaacac ggtcgccctt gttgtgggtt tccgcgaact gctgccacgg gttagctttg
    17821 cactgtttca gacccaggga gatacgacga cgttcttcgt cgatatccag aaccataact
    17881 tccactacat cgccaacgtt aacaactttg gacgggtgga tgtttttgtt ggtccaatcc
    17941 atttcggaaa cgtgtaccag accttcaacg ccttcttcga tttcaacgaa gcagccgtag
    18001 tcagtcaggt tggtcacgcg accagtcagt ttggtacctt ccggataacg tttagcgata
    18061 gctacccacg gatcttcgcc cagctgtttc aggcccaggg atacacgggt acgttcgcgg
    18121 tcgaacttca gcactttaac agtgatttcg tcgcccacgt tgacgatttc gctcggatgc
    18181 ttaacgcgtt tccaggccat gtcagtgatg tgcagcaggc cgtcaacgcc gcccagatca
    18241 acgaatgcac cgtagtcagt gaggttctta acgatacctt taacttccat gccttcctgc
    18301 aggttttcca gcagctgatc gcgctctgcg ctgttttcgg attcgataac ggcacgacga
    18361 gaaacaacaa cgttgttgcg cttctgatcc agcttgatta ctttaaattc aagctctttg
    18421 ccttccaggt gcagagtgtc acgcaccgga cgaacgtcta ccagagaacc tggcaggaac
    18481 gcacgaatac cgttcagctc aacagtgaag ccgcccttaa ctttgccgtt gataacaccg
    18541 gtaacagttt cagcatcttc gtaagctttt tccagcgtga tccaggcttc gtgacgttta
    18601 gctttctcac gggacagcag agtttcaccg aagccgtctt ctactgcgtc cagagcaacg
    18661 tcaacttcgt cacctacctg gatttccagc tcgccctggg cgtttttgaa ctgctcagcc
    18721 gggatggcgg actcagattt cagaccagcg tcaaccagta ctacgtcttt gtcgatagca
    18781 acaacaacgc cacgaacgat agaacccggg cgggtttcga tttcttttaa ggactcttca
    18841 aagagttgag caaaagattc agtcatgttt aatcttcagg tttatattta acgtccacct
    18901 ggctccatgc cggatggggt tgtttaacat acccgctgtc attccattgc aacgggggta
    18961 ctgcaaattc ggtcgcttat gcgagagcca atttctggcg cgcgtattgt agcgcttttt
    19021 caatcacttg ctcaatgctt aaggtggtgg aatccaacac taaagcatcg gatgccggaa
    19081 ccagtggcgc taccgctcgg ttacgatcgc ggtcgtcgcg ttctttgatc tcggccaaaa
    19141 ggcgctcaaa gttaacacta aagcccttct cctgcaactg tagcatgcgg cgatgcgcac
    19201 gttcttccga ggaggcgtca aggaaaattt tcactggcgc atcagggaat accaccgttc
    19261 ccatgtcgcg gccatcggca atcaggcctg gtaattcgcg aaacgcgcgt tggcgacgca
    19321 ataatgcttc acgaacgcgt gggaatgccg cgacttgtga agctgcattc gccacttcct
    19381 gagtacgaat ttcgccgctg acatcttccc cttcgaggat cacttccaga ttgccattgg
    19441 ttgacacaaa acgtacatcc agatgggatg ccagcggtac cagcgcatct tctgacgcaa
    19501 catcaacatg gtgatgtaat gccgccaacg ccagtacgcg ataaattgca cccgaatcca
    19561 gcagatgcca ttgcaatgct tccgccatag ccttacacaa ggtgcctttc cctgcaccgc
    19621 ttgggccatc aatggtaata accggggcaa ttgccgtcat ctttatctcc ttaaaacagg
    19681 cgtaccgtta acataaccgc gcgcattata cgcgccgacg ccacaaatcg ttaacgttgt
    19741 gtgcaaaaac cagttttgtc tgtattcaga tggggattta ttgcgcaatt atagcgggct
    19801 ggcagaagac cagcccgaaa gatgacaatg atttacttac cagaggcgat acgatcacgg
    19861 atgtgttgcg cacgctctgt tgatggtggg tgagagtcaa acatggactg ggtgcgaccg
    19921 ccatccagac tagccagttt ttcaaagctg ccaaccagcc cctgggtgct gatgccacgt
    19981 ttcttcaaca gatcaaagga gaaatcatct gcttcggact ctttatcacg ggagtattta
    20041 gcattaatag cgccttctgc gatatcaccc aattgtgagc gggaaagctg ggaagccaca
    20101 ccgctggtag ctgaaatggc atcgcgtgcg gcaacgatcg cataagaagc tttcatttca
    20161 gccagcgagt gacccaacgc gacgtggccc agttcatggc ccagaacacc ttcaatttcg
    20221 ttgtcattca tcatgtccat caggccactg tagacacgga cacagccgtt cgccatcgcc
    20281 catgcgttga cgtcgctggt catataaacc ttatagttga ccggcgtgcc attaatattg
    20341 ttacccaatg ctttggcgat ttttgccaga cgtttaccgt acttgctgga tttacttgcg
    20401 acttgattgc cgctgtccat ttcggcacag ccctgattgg caatcgcttt tgcatcggca
    20461 tcgcttaatg ttgctgcttt ataggcattt aaaccggagc tgatagccat attggtatca
    20521 ataccgtggg tattttgaca ccctgtcagt aatgctgcag aggtcgcaat cgccagcagt
    20581 aatttagtat tcttcatgtg aatcatccgt tgattaatca tatataaata ttatgtatca
    20641 acaacttaca ttgacaagcg acgctaccat taatcaaaat gactcacaag gtccgaaaaa
    20701 aaacgccgac aatg
//
LOCUS       DAJTVJ010000076        20435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000076 DAJTVJ010000000
VERSION     DAJTVJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.076"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            743..1171
                     /gene="uspC"
                     /locus_tag="OO448_002652"
     CDS             743..1171
                     /gene="uspC"
                     /locus_tag="OO448_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR6337828.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1178..2602)
                     /gene="otsA"
                     /locus_tag="OO448_002653"
     CDS             complement(1178..2602)
                     /gene="otsA"
                     /locus_tag="OO448_002653"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCR6337829.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2577..3377)
                     /gene="otsB"
                     /locus_tag="OO448_002654"
     CDS             complement(2577..3377)
                     /gene="otsB"
                     /locus_tag="OO448_002654"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6337830.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3544..4530)
                     /gene="araH"
                     /locus_tag="OO448_002655"
     CDS             complement(3544..4530)
                     /gene="araH"
                     /locus_tag="OO448_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6337831.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRIV"
     gene            complement(4545..6059)
                     /gene="araG"
                     /locus_tag="OO448_002656"
     CDS             complement(4545..6059)
                     /gene="araG"
                     /locus_tag="OO448_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6337832.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6129..7118)
                     /gene="araF"
                     /locus_tag="OO448_002657"
     CDS             complement(6129..7118)
                     /gene="araF"
                     /locus_tag="OO448_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6337833.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7915..8418
                     /locus_tag="OO448_002658"
     CDS             7915..8418
                     /locus_tag="OO448_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6337834.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8497..8748)
                     /locus_tag="OO448_002659"
     CDS             complement(8497..8748)
                     /locus_tag="OO448_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6337835.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8863..8949)
                     /gene="azuC"
                     /locus_tag="OO448_002660"
     CDS             complement(8863..8949)
                     /gene="azuC"
                     /locus_tag="OO448_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6337836.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9212..9535
                     /locus_tag="OO448_002661"
     CDS             9212..9535
                     /locus_tag="OO448_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6337837.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9706..10203
                     /gene="ftnA"
                     /locus_tag="OO448_002662"
     CDS             9706..10203
                     /gene="ftnA"
                     /locus_tag="OO448_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6337838.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10241..10480)
                     /locus_tag="OO448_002663"
     CDS             complement(10241..10480)
                     /locus_tag="OO448_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6337839.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10671..11882
                     /gene="tyrP"
                     /locus_tag="OO448_002664"
     CDS             10671..11882
                     /gene="tyrP"
                     /locus_tag="OO448_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6337840.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11944..12609)
                     /gene="yecA"
                     /locus_tag="OO448_002665"
     CDS             complement(11944..12609)
                     /gene="yecA"
                     /locus_tag="OO448_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6337841.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12805..12891)
                     /locus_tag="OO448_002666"
     tRNA            complement(12805..12891)
                     /locus_tag="OO448_002666"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12855..12857),aa:Leu,seq:taa)
     gene            complement(12904..12977)
                     /locus_tag="OO448_002667"
     tRNA            complement(12904..12977)
                     /locus_tag="OO448_002667"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12943..12945),aa:Cys,seq:gca)
     gene            complement(13032..13107)
                     /locus_tag="OO448_002668"
     tRNA            complement(13032..13107)
                     /locus_tag="OO448_002668"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13072..13074),aa:Gly,seq:gcc)
     gene            complement(13259..13807)
                     /gene="pgsA"
                     /locus_tag="OO448_002669"
     CDS             complement(13259..13807)
                     /gene="pgsA"
                     /locus_tag="OO448_002669"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6337842.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13864..15696)
                     /gene="uvrC"
                     /locus_tag="OO448_002670"
     CDS             complement(13864..15696)
                     /gene="uvrC"
                     /locus_tag="OO448_002670"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6337843.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15693..16349)
                     /gene="uvrY"
                     /locus_tag="OO448_002671"
     CDS             complement(15693..16349)
                     /gene="uvrY"
                     /locus_tag="OO448_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6337844.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16645..16821
                     /locus_tag="OO448_002672"
     CDS             16645..16821
                     /locus_tag="OO448_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337845.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16808..17032
                     /locus_tag="OO448_002673"
     CDS             16808..17032
                     /locus_tag="OO448_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6337846.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17099..17821)
                     /gene="sdiA"
                     /locus_tag="OO448_002674"
     CDS             complement(17099..17821)
                     /gene="sdiA"
                     /locus_tag="OO448_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR6337847.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(18051..18803)
                     /gene="yecC"
                     /locus_tag="OO448_002675"
     CDS             complement(18051..18803)
                     /gene="yecC"
                     /locus_tag="OO448_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6337848.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(18800..19468)
                     /gene="tcyL"
                     /locus_tag="OO448_002676"
     CDS             complement(18800..19468)
                     /gene="tcyL"
                     /locus_tag="OO448_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6337849.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19483..>20435)
                     /gene="dcyD"
                     /locus_tag="OO448_002677"
     CDS             complement(19483..>20435)
                     /gene="dcyD"
                     /locus_tag="OO448_002677"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6337850.1"
                     /translation="EFIGAPTPLEYLPRFSDYLGREIFIKRDDVTPMAMGGNKLRKLE
                     FLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENPIGTTAENYLTNG
                     NRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGGSNALGALGYVES
                     ALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGVTVSRSVADQLPK
                     VVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLARLEGILLDPVYT
                     GKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         5220 a         5210 c         4703 g         5302 t
ORIGIN      
        1 gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa
       61 tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt ccttacaaat
      121 agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca
      181 ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat gcagatcaca
      241 caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg ggtgtatttc
      301 aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga ataatcgaaa
      361 tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta taaataatca
      421 ctttcatgca ataccagata agctattttt aaacagacac ttaccacaca acaaactaat
      481 taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac atacattaac
      541 cacttgttta atgcggagaa tcagtacact attttcttat aattacattt gaaattatat
      601 gatcaccggg agtgattgat taattgtctt tactaataat cagactaata tttacctgtt
      661 tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg tcacctgaag
      721 ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg ttgcggttac
      781 accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag taaagggaca
      841 catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag ccgcgccgat
      901 gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg ataagttcat
      961 tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt taagcgaaca
     1021 tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta atcacaatca
     1081 cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa gtgaggtcga
     1141 tgtgctgtta gttccactta cgggagatta accgctccta cgcaagcttt ggaaaggtag
     1201 caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt aggtcgctaa
     1261 tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc atttctgcat
     1321 gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca gctgcaactt
     1381 cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc gctcccgcaa
     1441 attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca tactcttttg
     1501 ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag tagcggaata
     1561 ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc gtccagccta
     1621 attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga cgaatatcct
     1681 gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga attttaccat
     1741 gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct ggcaaacctt
     1801 tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt tccgctttaa
     1861 gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct ttcggttcaa
     1921 tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg ctttttgcgc
     1981 tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga cgatcgtttt
     2041 ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg tcatatgtcg
     2101 gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa ccaatgcgat
     2161 tattcactcc ccgtttgcgt aattcatgcg caaatggcaa caggtgataa tcgtggatcc
     2221 agataatgtc atcgtcttgc aacagcggca gtaatttatc tgccagcaac gcatttacgc
     2281 gtagatagcc gtcccaggca ggacgctgaa attgcaccag atcgagccga taatgaaaag
     2341 cgggccagag aacggcattg gagaattggt tgtagtattc gtcaaggtcc tgttcgctga
     2401 ggttaaaaga ggcccacgta atgttacctt ttttcacctt ttttagcggc tgatcctcat
     2461 tccctgtttc accactccag ccaaaccaca gtccgcctgc ggctttcagt gcccccagta
     2521 tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca atccggttag
     2581 atactacgac taaacgactc atagtcatca ctcctgttat tttctctttt ttgttgtaat
     2641 gcggtggtta tcatttcaag ccagctccag acatccggca cacccgccag tcgccatgat
     2701 gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt aacgactgcg
     2761 aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc gataaaggga
     2821 gcttcctgca taaaagctgc aattgcctca cctttactgg tacctctcgg tttgatctcg
     2881 acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat acgttgcgct
     2941 aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc aaaagccatc
     3001 cctttcgcct ccagctccgc gccgggatac tgagcgatga ctgtatgcag ttgcacgcta
     3061 atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt gatgtcacgg
     3121 cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag tgcgtcaagc
     3181 tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt tgccagtagc
     3241 tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg tttgatttcc
     3301 gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc aggggtttcg
     3361 gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtttt gcgcagaacg
     3421 cgctttacag acatgtaagt atagacagtg tgacgggggt cgccatttgc aatgaagaac
     3481 agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt gtggaaaaaa
     3541 gcatcagaca atgcgtttcg ctttttgctt gtaacggtcg aagatcaccg ctgccagcag
     3601 gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt tcatggcgtt
     3661 ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc cgatgccacc
     3721 tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat aaccaatcga
     3781 cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta tcgcagatac
     3841 caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac ccgccagacg
     3901 cgcagcctct tcgttcccgc caatcgccag ggtgttacga ccaaaggtgg ttttattcag
     3961 caacaaacca aagataatca gacacgcgac ggtgagccag attggcgcag gcagaccgaa
     4021 ccagttagcg taaccaaggg caaagaagct ttcatcttcg ataccgaccg ctttaccgtc
     4081 tgaaatgatg tacgccagac ctcgaacaat ctgcatcgtt gccagtgttg tgatcagagc
     4141 atttattttc agtttggcga taacaaagcc attgaccagg ccacagagaa tgccaagcag
     4201 caaccccgct gccacgccaa tccacaggct ttcagtcagg ttgataacca ccgccgtggt
     4261 gacacccgca caggcaatta cggaggcgac agaaaggtca aagtcaccgg aagcaaggca
     4321 gaacaacatg ccacacgcca ccatccccga catggaaatt gccaggccca accctttcat
     4381 attaatgaag gtggcaaaat ttgggacaaa aatggcacag gcgataaaga gcaccgcaaa
     4441 caccaccagc atgccgtact gatcccagat acgcccgaag ctgaatgacg acttaggtgc
     4501 gccagaaccc gatgtagaaa cagaagacat catactctcc ttactcaggc aactgcctgg
     4561 ctgactttag gcatcgcaag gctcagtgcc tgacgctcat ctgcctgctc gtgtaacaat
     4621 tcaccggcga tttcaccttc ccgcatcacc acaatccggt cggcaacgcc gaggacttca
     4681 ggtaagtcgc tggaggcaaa cagcaccgcc acaccctgcg ccgccagcgc ataaattacg
     4741 ttatatattt cgtgcttagc accaacatca atgctgcgcg taggttcatc cagcaaaatg
     4801 accttcatct cttccgataa ccagcggccc agaatggctt tttgctgatt tccgcctgag
     4861 agattcatga tcagttgctc agcgcccggc gttttgatgt tgagcgaacg aatgtgctga
     4921 tcggcattgt tttcttccca accgttgttg attacacaac cgccgagcac atgtttgcgt
     4981 ctggcactga tgttgatatt gtcgcgaacg gagtgcacgg gaataatgcc ttccgctttg
     5041 cgatcttccg ggcagagcat catgcctgcg gcaatggcgt ggctcggttt acgaatatcg
     5101 atcggctgtt ggtcgatata aacctgaccg gcggtgattt gcgtcccgcc aaacaagcct
     5161 ttcattaatt cgctacgccc cgcccctacc agaccaaaca gcccaacaat ttcaccactg
     5221 cgaaccgcca gacttattgg cgtacgcacg cctggtgctt tcacagcatc aagacgcagg
     5281 cgctcctcgc cataactacg aggttgccag ccgtagatat cgccaatgtc gcgcccgacc
     5341 atcgcctgca ccagcgcgtc gtggtcaacc tgctgcatat cggtaaaggt tttgacataa
     5401 cgtccatctt taaagacagt gatggcatcg ctgagggcaa atatttcttc catacggtga
     5461 gaaacgtata agatcacccg cccctctttt cgcagttcac gaataacgcg gaaaagattg
     5521 tcgatttcgc gggcagaaag ggagctggtt ggctcatcaa aggcgataat tttagcgtta
     5581 cgcgccagcg ccttggcgat ttcaaccatc tgccactgac caatggagag atatttcagc
     5641 ggcgtgtccg ggtcaatatc cataccaaga tgtttaagtt gtaaacccgc ctcataattc
     5701 agcaatgagc gattcacaat gccgccttta tgcggcagct ggccgagata gatgttttcc
     5761 gcgacggtca tttccggcac gagatgcagt tcctggtaaa taatcgccac acccgcgtta
     5821 agtgctgcgg tcgtgtcgga aaaggacatt tcctgcccat taatcactac agaacccgtg
     5881 gttggcgcat agttgccgct gaggattttt aagagagttg attttcctgc gccattttca
     5941 cccatcaacg catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta
     6001 acgccgggaa acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata
     6061 acgtctccgt gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt
     6121 ccggcaaatt acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc
     6181 gtgatcagca ccacgtcggt aacttcggta aattttggcg gttcaacgtc ttttgctacc
     6241 cagttgtaaa gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa
     6301 ccgtagaagc cggttgcctg tgctttagac agttcgctca acgcatccac accgttaatg
     6361 ccaatgccga tgatatcggc agctttaaag ccctgacctt ccgtcgcgcg tacgccgccc
     6421 agcacggtgc tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc
     6481 agcattgagt tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga
     6541 taaatttgtt tttccgggaa tccggccgct ttcagcgcat ccatagaacc cgttgtacgg
     6601 cgacgtgccg tatccagttc gttggcggta atcgccatca ccgcgctttc tttgacatcc
     6661 cagccacgtt tctgcatctc tttatacagt tcctgaccct gacgttcgcc aattttagtc
     6721 gccgccatca tcaccagcgg aacggtatcc attggcttac ctttggcgtt aacaaactgg
     6781 tcatccacgg caatgacttt catatcgtag ccacgcgctt tcgcgacgat ggcagagccg
     6841 agtttggggt ccggagtaca aataacgaaa ccttttgcgc cactggcagc caggctgtcg
     6901 atcgcgttca atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa
     6961 tccttcccgg ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc
     7021 ttcaccagaa aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc
     7081 agaccaatgg ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc
     7141 gttttgtgta gggcaaaaac gaatgacttt cgttaaatta atcggaaaac aaagcattac
     7201 cttttatcta aaagataagt gaccgtgttg acatagtttt agcgagaaat taattctcca
     7261 taggagagca atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa
     7321 agacataagg gaaagccaat ttgtcagaca aattgtcgaa tgcacagcag attaatccat
     7381 aagattagcc tggaaatcct tgttgtcttt ggtacccatg cgggatgtct tctttttaac
     7441 cagtcaatag gccgcattac ctggcgttga gtttttgaaa tggtgtaata accgcaactc
     7501 aaagatgtgg aaaatgcacg tcattcattt cgccattaat tatcactgtg ctcattaatt
     7561 aacagaacac gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat
     7621 cacttcatca tgaattcaaa ttcattgatt aatatcaaca agatacaaaa agcactatca
     7681 ttaaaattca ttgcagttac attgatttca tcaatgaaat gtaaaaatat ataaacttga
     7741 tgatttaagc attttcttat acccgttcag acgttattct tatttcagat catcgtcaga
     7801 attgactcca cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta
     7861 tggtgttttg atttatcttg cacctctcca cttctggata taaggatatt aggtatggca
     7921 accgctggaa tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat
     7981 ctctaccttc acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc
     8041 cttcgcgccc aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag
     8101 agtgtcggcg ctacccccat cgttaaagcc attgatgttc ccggtgaaaa actgaactct
     8161 ctggaagaac tgttccaaaa aacgatggaa gaatacgagc aacgttctag tacgttggca
     8221 cagttagccg atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat
     8281 ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa ccattcttga tgaagtgcgc
     8341 agtgcgaaac ttgcgggtat gtgccctgtg cagaccgacc aacatgttct gaatgtcgtg
     8401 tcacaccagc tgcattgatc atcatcggcg ctaatgcatt gcgccgatga aggttttgag
     8461 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     8521 aaacttgagt tcaataagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     8581 ggcggcggga tggttagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     8641 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     8701 aaccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     8761 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     8821 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     8881 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     8941 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     9001 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     9061 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     9121 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     9181 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     9241 aataacgctg gcggggtgta cggttactcg ccaagcccat gtgagcgaag ttgacgcggc
     9301 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     9361 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcggt
     9421 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     9481 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     9541 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     9601 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     9661 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     9721 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     9781 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     9841 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     9901 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     9961 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
    10021 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
    10081 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
    10141 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
    10201 taatgctctc cggcggcagg tttactgccg ccgtttcaaa tcagtgacgg caaatcttac
    10261 tctgatcggt cgaaaaaccg tctttcgaag gtataaattt gccttttgct gccagaaacg
    10321 ctaccagctc tcccgctgtc atcccttctg ccgagcaggt gtgaaaacgt gcctgttcac
    10381 caaaacgcgc tttaatcgca gcttccagac tggcatgcgt atattgctcg cctgattcaa
    10441 tcatcatatt taacacttca tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc
    10501 ctagcgtagc gattgccgct tatgaagact ttgcgccagc gcaggactga atgcttttta
    10561 ttgtacattt atatttacac tatatgtaac gtcggtttga cgaagcagcc gttatgcctt
    10621 aacctgcgcc gcagatatca ctcataaaga tcgtcaggac agaagaaagc gtgaaaaaca
    10681 gaaccctggg aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc atgctggcaa
    10741 tgccgctggc tgcggccggt gttggtttta gcgttacgtt aatcttgttg attgggcttt
    10801 gggcgttgat gtgctacacg gcgctattac tgctggaggt gtaccagcat gttccggcag
    10861 ataccggtct gggcacgctg gcaaaacgct atctgggacg ctacggtcaa tggctgacgg
    10921 gcttcagtat gatgttctta atgtatgctc tgactgcggc atacatcagc ggtgtcggtg
    10981 aattgttggc ctccagcatc agcgactgga caggtatttc aatgtcggca accgctggcg
    11041 tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt tggaacatcg ctggtcgatt
    11101 tatttaaccg ttttctgttc agcgccaaaa ttatttttct ggtggtgatg ctggtattgc
    11161 tgctaccgca tattcacaaa gtgaatcttt taaccctgcc gttgcaacag gggctggctc
    11221 tgtcagcaat cccggtgatt tttacctcat ttggttttca cggtagcgtg ccgagtattg
    11281 tcagctatat ggatggcaac gttcgtaagc tacgctgggt gtttataacc ggtagtgcga
    11341 tccccctggt ggcatatatt ttctggcagg tggcaacgct tggcagcatt gattcaacaa
    11401 cctttatggg attgctggct aatcatgctg gattaaacgg gctgttacag gcgttacgcg
    11461 aaatggtggc ctctccgcat gttgagctgg cagtgcattt atttgctgat ttagccctcg
    11521 ccacgtcatt tctcggcgtt gcgttaggct tatttgatta tctggctgat ttatttcagc
    11581 gttcaaatac cgttggtgga cggttgcaaa ctggtgcaat tacctttctg ccgccgttgg
    11641 cgtttgcact gttttatcca cgaggatttg tgatggcgct gggttacgcc ggtgtggcgc
    11701 tggcggtact ggcattgatt atcccttcgc tgttgacctg gcaaagcaga aagcacaatc
    11761 ctcaggcggg ttaccgggtc aaaggtggtc gtccggcgct ggtggtggtg tttctctgtg
    11821 gtattgctgt gattggcgtg caatttttga ttgcggcagg gttgttacca gaagtggggt
    11881 gatcagatag cctcaaattc cttattgggt gccagaatta acgctgacac ccaatttggc
    11941 ctcttaatgc aggcagcact gcttaaattt cttaccacta ccgcacgggc aaggatcgtt
    12001 acgccccggt ttctcttctg ctttgatcgg ttgctgaaca gctttttcct gcggatgcgc
    12061 catccagtac gcatgtagat caagcgccgc cagtcgaatg gcatctacgc tctcttcaaa
    12121 cgcttctggc gacatctttt ctacccgctc gaagttttcc tcagtaccgt gcagcgcaat
    12181 cgcctccagc gctggtttta acgaatcggg caacgttgac cagtcagaaa gtgccacgcc
    12241 ccgcatatag ccaaagcacc actcctcaac aatcgtcagc tcgctgccat caacttctcg
    12301 caagccgaat aacggctcaa actgctccgg gaattcgttc agacgctctg cggtatcggc
    12361 catatgttga aaagccaggt tcataaagcg cgtcatctct ttctctgacg cccagcgcgg
    12421 cacatagtca gccccacccc acacggcaac cagccactgt tccggttcaa tctcttgcgg
    12481 agaactcaac accgccgtca ataaaccgtc cagctccgcc acatcaagga tggcgtggtt
    12541 agtgttgtat ttggtcagaa tatcgtccag ccattccaac tcactttcgt ttaacggtcc
    12601 cgttttcata cgcttttcct tgtggatctc aactcgccag cacctatctt acatgccggt
    12661 ccgtatcaga gatacttttt gagtggcttt gccggtgatt aaaaattaag gagggtgtaa
    12721 cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt tcacgacact gcttattgct
    12781 ttgattttat tcttatcttt cccatggtac ccggagcggg acttgaaccc gcacagcgcg
    12841 aacgccgagg gattttaaat cccttgtgtc taccgattcc accatccggg ctcgggaaga
    12901 aagtggaggc gcgttccgga gtcgaaccgg actagacgga tttgcaatcc gctacataac
    12961 cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca gacatccgct tgacgccgat
    13021 gtcttttaaa ctggagcggg aaacgagact cgaactcgcg accccgacct tggcaaggtc
    13081 gtgctctacc aactgagcta ttcccgcatt catcaagcaa tcagttaatc acttgatttt
    13141 attatcgtct ggcaatcagt gccgccgttc gatgcgttgc attctactta cctggcgcga
    13201 tgagtcaacg atatttttca ccacttttga tcgtttgctg aaaattacgc cgaaacgatc
    13261 actgatcaag caaatctgca cgcgcagcgc tcaaatattg caacattgac cacagagtca
    13321 gtaccgcagc cacaaagaaa agtgcaatac cggcgtactc aacccaaatg ttcggacgcc
    13381 acagcagcca tgccaacgcc accatctggg cagtggtttt cactttccca atccaggaga
    13441 cagccacgct actgcgttta cccaactccg ccatccattc gcgtagcgca gaaataataa
    13501 tttcacgggc gatcatcgtt gctgccggta aggtcaccca ccagctgtgg taatgctcgg
    13561 ttaccagcac catggcgata gccacgagaa ctttatctgc tacagggtca aggaaagcac
    13621 caaaccgggt actctggttc cagcggcgtg ccagaaaacc atcgaaccag tcagtcaccg
    13681 ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc agataaaaga
    13741 ccaatacaaa gaatgggata aggatgacac ggaacagtgt aagcaacgta gggatattaa
    13801 attgcataat gacgggtaac tatctgttgt cagtaagatt acccctatgt tgctacagag
    13861 acatcaatgt ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa tacccggcac
    13921 ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc aaaccgccca tatatttcaa
    13981 caacatttgc cgacgttttg gcccgacgcc ttcaatggtt tccagggaac tggtattttt
    14041 gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga tcatgtgatt catcgcgaat
    14101 atgctggata acatgcagcg cgggagaatc tggcggcaaa ctgaatccct caccttccgg
    14161 ctcaaagaac agcgtttcca gcccagcctt acgatctgct cctttggcaa cgccaagtag
    14221 cagcggatga tttttatccc atgagacatc cagttcggcg aagacatttt tcgcctgcgc
    14281 aagctggcct ttgccgccgt cgataaggat cacatccggg atcttactgt cgtcaatggc
    14341 tttaccataa cgccgacgca gcacctgatt catcgccgca taatcatcgc ccggcgtgat
    14401 gcctgtaatg ttatagcgcc gatactccgc acgcagcggg ccgttagcat caaacaccac
    14461 acaggaagcg acggtttgtt cgcccatggt atggctgatg tcaaagcact ccatccgctt
    14521 cacttccggc aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa cggtagattg
    14581 ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt tcagataacg
    14641 cgccctatcg ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca gttctgaaag
    14701 ggaatcggcg agcagcgttt tatcgctaag attaaaatcg agcaggatct cacccggtaa
    14761 ggtgcgcatc tggctgcctt gtaaatagaa ctggcctacg aaggtttcta ccacctcgct
    14821 cagttccgta ccgccaggca ctttcgggaa atagctgcgg ctgccgagca ctttgccctg
    14881 acgaatgaac aatacgtgga cacaagccat gcccgcatcg aacgccacac caataacgtc
    14941 gaggtcgtcg ccggtattgg aaacgaattg tttttcggtg acgcgtcgca ccgcctgaat
    15001 ttggtcgcga atacgtgcag cttcttcaaa ctccagattc tggctggcag tttccatacg
    15061 actaatgagt tgcgtaagca cctgatcatc tttgccagac aaaaacaggc gcacatactc
    15121 aacctgctga gcgtattctt cttcactcac cagtccttca acgcacggtc ccagacagcg
    15181 ccctatctga tattgcagac acggacgcga gcgattgcga taaacactat tttcgcactg
    15241 gcgaatgggg aaaatctttt gcagtagcgc cagtgtttca cgtacggcat agccattcgg
    15301 gaacgggccg aaatattcac ctttggcatg cttcgcacca cgatgcatcg ccagacgcgg
    15361 gtgggtatcg ccactcagga agataaaagg ataagattta tcatcgcgta gcaaaacgtt
    15421 gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc aacagcgctt cggtttctgt
    15481 atgcgtaacc gttacatcaa tttgctggat ctgggcgacc agcgcttcgg ttttgcgcga
    15541 agcgaggttg ctgcggaaat agctggaaag ccgttttttc aggtctttcg ctttgccgac
    15601 atagataacc gtaccaccag catcgtacat gcgataaacg cctggctggc aggttacggt
    15661 ttttaaaaac gcttttgcgt caaactgatc actcactgac ttgataatgt ctccgcatta
    15721 cacagaccat ggcgaattgc caggtgagtc agctcaacat cgccatgaat gtttagttta
    15781 ctgaacatac gatagcggta gctgttcacc gttttcggac tgagattgag ctgttctgag
    15841 atctcattga ccttctggcc cttggtgatc atcagcataa tctgcaattc acgttcagac
    15901 aaactggcaa atgggctttc tgttttttct ggttcgatct ggcttaacgc catttgttga
    15961 gcgatatcag aagcaatgta acgctgccct gaatagacag aacgaatcgc actcacgact
    16021 tcctgcggag ccgcgccttt gctgaggtag cccgcagcac cggcctgcat gactttcgct
    16081 ggtaaagggt tttctgtatg gacggtaagc atgatgattt tgacatcagc tgtggaacgc
    16141 gcgattttac gcgtcgcctc aagaccgcca atgcccggca tactcatgtc cattagcacc
    16201 acgtcaacgg catttgtccg gcaccactta acggcgtctt caccgcacga tgcctcaccg
    16261 acgactttta taccctttat atcttccaga atgcgtcgta tccctgcgcg caccagttcg
    16321 tggtcatcaa caagtagaac gttgatcaaa ggaatatctc cagaaatagg gataacgcta
    16381 ctgatagtta gtcattcgta tattagcggt ttttattgca actttgaaac gttaaaaatg
    16441 gtgggtattc gatttttctc tcgtttttgt aaattcgatt gtccacagtt atggtcacgc
    16501 ccgtcaccaa cggcttccgg gatcgtttca cagtaagccc ggcgatgaaa tgtgacaaaa
    16561 attgacttta ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat taattaaccc
    16621 gaacaatacg ggcaaaaaac attcatgata aaaatattta ttggtcatta tattaacgca
    16681 tttaatagca ttgttgacgt cacgctcaaa aaacaaccac tgctattttt agcaaagctt
    16741 atggtatact ccgccgcctt aacatttttc accgcaaatt ttcattgcaa catgacgagg
    16801 aaaataaatg agtacgcctg atttttctac tgccgagaat aatcaagaac tggcaaatga
    16861 agtctcctgc ctgaaagcga tgctgacgct gatgctacag gcgatgggac aagctgacgc
    16921 gggccgcgtg atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc
    16981 tgcagtattt tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt aatataaaac
    17041 cggctgagag cgtgcctttc agccgatttt tgcatctggc acgcagaaag aaaagcgatc
    17101 aaattaagcc agtagcggcc gcgtaacagg ccacctgggt cttatttggt gcattgattt
    17161 ttttctgcat gtttttctga tggaaattga ccgtattctc agagattgac aaaatcatcg
    17221 ctatctctgc tgatgtcttc ccttccgccg tccatttcag aatttctttt tcgcgcttgc
    17281 tgaaattcat ctctggcgtc atcactattt catcatttaa acgcatcaga gccatcagac
    17341 tttcgcgcac cagtaactgc atttttaatt gcagttcatc actaagaatg ggtatttcgc
    17401 gcgtgctgca acgggaaaag gacaaaaagc ccagcgcccg atttggcagc attaaatact
    17461 gagtgacacc gcggcgtaaa ccatgtgcgt gcgcggcttc ccataatggc tgtgcttcgc
    17521 tgaataagtc atcattccac attaaatggc cctgactaaa gttttcaggg ttcagcacag
    17581 gatcaattgc gagaaaattt tttgcctgat aataactaac ccacgactca gggtaattgg
    17641 tgtagaaagc cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt
    17701 aatcgtactc cagctgctga gcctgaagct ctatttcatg gtagacctct tctgcggcct
    17761 ccatcctctg aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca
    17821 tagtaaaccg caacgcccct gagagtgagt atcatttata atgataattc aaactatcac
    17881 ataaataaga tttatatata atttatatta ttcaggcaat gaattacttt tgcaagccat
    17941 cgcattctct tatgttatta atgagttatg ctgatttgtt aagcagtttt atcacgcttg
    18001 aaatggcgtc ccgccccgac aggtgaatcg tcggggctga ttttttctta ttattgcagt
    18061 agaaacttct cgaggaactg gcgggtgcga ggctgctggg gatcagcaaa taacgctttt
    18121 gctgccccct gctcgactat ccgcccctgg tccataaaaa tcgcccggtc cgcaacatcc
    18181 cgggcaaagc tcatttcgtg cgtcacaatc accatcgtgc gcttttcctg cgccagctgg
    18241 cggattgtgt tcaggacttc gcccaccagc tctggatcca gcgctgacgt tggctcgtca
    18301 aacaaaatca cctcaggacg cattgccagc gcacgcgcaa tcgctacacg ctgctgttga
    18361 ccgccagaca aacgacgtgg atagctggtt tctttaccgg ccagcccaac tttcgccagc
    18421 agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat caccggccct
    18481 tcaataatgt tttccagcac cgtacgatgc ggaaacaaat taaagttctg gaagacaaac
    18541 ccgacgtgct gacgcaactg gcggatcagc gatttttgct gacttaatga acgtgcagta
    18601 tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg gttgttccag cagatttatg
    18661 ctgcgtagca acgtggtttt gccggaacca ctcggaccga taattgccac cacttcgcca
    18721 ggctttacct cgaggtcgat accgtgcagc accgtctgac cgtgaaattt tttcaccagg
    18781 ttcttaactt caatggcact cattttggtt ctctctcctg gcgattaagt tgattctcaa
    18841 aatggttctg caacgtcgat aatactgtcg ccatgatcca gtagatgagc gaagccgcca
    18901 ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct
    18961 ccggcacctg gattgtcgcg gccagcgacg tatcctttac caggctgata aagctgttcg
    19021 acagcggcgg caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc atagtctgcc
    19081 atggcgtcat accaatactg gcagctgctt cccactgacc tttatcaata gaagaaatgg
    19141 cggcgcgcag tgtttcggcg gcataggcgg cagtgttcag cgacagacca atcattgctg
    19201 acggaatggg atctaattcg ataccaaact gcggcaggcc gtaatagatc ataaacagtt
    19261 gtgcgatgag cggtgtacca cgaaaaatag agatataaaa acgcgccagc cagcgcacgg
    19321 gccagattgg cgacaggcgc atcagcgcga gaataaaccc gagcagtaag ccaaaaaaca
    19381 tgccgccgat actgagttgc agcgtatacc cagccccttt cagcaggaac ggcaaggaat
    19441 caataaccag ttgtatactt tcttgcatga gcgttcccgg agctaaacgt ggggatgata
    19501 ggcgaacagc gcaggcgcac cgccggtatg aataaacaga atcggccctt catctttgaa
    19561 gcgtttctga ctgatacagc caatcagccc cgccatcgct tttccggtat acacaggatc
    19621 aagcagaatg ccttccagcc gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg
    19681 cacgccgtag ccaggtgcaa aatagtcatc ccagagtata atttccgctg atgcggtcag
    19741 ctccagttct ttcgcaatcg cctgttgtag gttaaccact ttcggcaact gatcggcaac
    19801 ggaacgcgac acggtcacgc caatcagttc gctttcaggc atcaggtgtt ccagcccaac
    19861 agccagtccg gcgtgagttc cggcactgcc cgatgcgact accaccgacg aaatattaac
    19921 cgccccttca cactgttgcg cgatttccag cgcactctcc acataaccta gcgcgccaag
    19981 agcattagaa ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt
    20041 cgccagctct tccagttggg cattgggatc ggtcagtgct tcgcacattt caatctgggt
    20101 attgaacaga tccagcaaca agcgattgcc gttggttaaa tagttttctg cggttgtgcc
    20161 aataggattt tccagcagcg ccacacagtg cagaccgagt ttcgccgcaa ctgcggcagt
    20221 ctgccgcaca tggttagact ggatcacccc ggcagtaatc agcgtatcgg caccttcgcg
    20281 cagagcatct gccgcgagaa attctagctt acgtaattta ttgccgccca ttgccatggg
    20341 ggtgacgtca tcccgtttga tgaaaatttc ccgtcctaga taatcagaaa agcgcggcag
    20401 atattcgagc ggcgttggcg cgccgataaa ctcca
//
LOCUS       DAJTVJ010000077        20005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000077 DAJTVJ010000000
VERSION     DAJTVJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.077"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="OO448_002678"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="OO448_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6337851.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="OO448_002679"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="OO448_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6337852.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="OO448_002680"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="OO448_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6337853.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="OO448_002681"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="OO448_002681"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6337854.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="OO448_002682"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="OO448_002682"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6337855.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="OO448_002683"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="OO448_002683"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6337856.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="OO448_002684"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="OO448_002684"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6337857.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="OO448_002685"
     CDS             5315..6439
                     /locus_tag="OO448_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6337858.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="OO448_002686"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="OO448_002686"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6337859.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..8261
                     /gene="waaO"
                     /locus_tag="OO448_002687"
                     /gene_synonym="rfaI"
     CDS             7245..8261
                     /gene="waaO"
                     /locus_tag="OO448_002687"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6337860.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8278..9273
                     /locus_tag="OO448_002688"
     CDS             8278..9273
                     /locus_tag="OO448_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR6337861.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9283..9975
                     /gene="rfaY"
                     /locus_tag="OO448_002689"
     CDS             9283..9975
                     /gene="rfaY"
                     /locus_tag="OO448_002689"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6337862.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10001..11026
                     /locus_tag="OO448_002690"
     CDS             10001..11026
                     /locus_tag="OO448_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR6337863.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11111..12094
                     /locus_tag="OO448_002691"
     CDS             11111..12094
                     /locus_tag="OO448_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6337864.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12140..13393
                     /locus_tag="OO448_002692"
     CDS             12140..13393
                     /locus_tag="OO448_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6337865.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13463..14434)
                     /gene="rfaC"
                     /locus_tag="OO448_002693"
     CDS             complement(13463..14434)
                     /gene="rfaC"
                     /locus_tag="OO448_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6337866.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14438..15484)
                     /gene="rfaF"
                     /locus_tag="OO448_002694"
     CDS             complement(14438..15484)
                     /gene="rfaF"
                     /locus_tag="OO448_002694"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6337867.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15494..16426)
                     /gene="rfaD"
                     /locus_tag="OO448_002695"
     CDS             complement(15494..16426)
                     /gene="rfaD"
                     /locus_tag="OO448_002695"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6337868.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16640..17836
                     /gene="kbl"
                     /locus_tag="OO448_002696"
     CDS             16640..17836
                     /gene="kbl"
                     /locus_tag="OO448_002696"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6337869.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17846..18871
                     /gene="tdh"
                     /locus_tag="OO448_002697"
     CDS             17846..18871
                     /gene="tdh"
                     /locus_tag="OO448_002697"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6337870.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTVICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19110..>20005
                     /gene="waaH"
                     /locus_tag="OO448_002698"
     CDS             19110..>20005
                     /gene="waaH"
                     /locus_tag="OO448_002698"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6337871.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5398 a         4361 c         4725 g         5521 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacggacg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atgctaaata caatactcaa tttagtttaa
     7981 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
     8041 atgtcggccc gacaaaaccc tggcattact gggccggtta tccaagtgcg caacctttta
     8101 tcaacgccaa agaagcatcg ccctggaaaa atgaaccgtt tatgcggcca attaattcaa
     8161 actatgctcg ttattgcgcc aagcataatt ttaaacaaaa tgaaccaatt aacgggataa
     8221 tgaattatat ttattatttt tatttaaaga taataaaatg atattgagaa aagttaaatg
     8281 aatgaattta taaaagaacg gttttcgtat ttagcagata ataaaaaaga aaacgcccca
     8341 gaactaaatg tttcctacgg tatcgataag aattttttgt atggtgctgg cgtttcaatt
     8401 tcttccgttt tgattaataa ttcagatatt aattttgtct ttcatgtttt cactgattat
     8461 gtggatgatg attatttaaa gtcatttaat gaaacagcaa aacaatttaa tacctcaatt
     8521 attgtatatt taattgaccc aaaatacttt gctgatctgc cgacgtcaca gttttggtcg
     8581 tacgcgacat acttcagggt attgtccttt gaatatctga gtgaaagtat ttccacactg
     8641 ctgtatctgg atgccgatgt cgtttgtaaa ggaagtctga agcctctcac agaaattata
     8701 tttaaagatg agtttgctgc ggtcattcct gacaatgtta gtactcaggc ggcatgtgca
     8761 aaacgcctca acattcccga gatgaatgga cgttatttca atgcaggcgt tatctatgtc
     8821 aatattaaaa aatggcatga agcaaatttg acaccgtatt tactcacgct tttacgaggg
     8881 gaaactaaat atggctctct taaatattta gatcaggatg cgttgaatat cgcatttaat
     8941 atgaataata tctacctcgc gaaggatttt gatactattt ataccctgaa aaacgaactt
     9001 catgatcgta gtcatcgaaa gtatcagcaa accattaccg ataaaacagt attgattcac
     9061 tatacaggga taactaaacc atggcatagc tgggctggtt atccgtctgc atcatacttt
     9121 aatatcgcgc gtgaacaatc tccctggaag aaatatcctc ttaaagaggc gcggactgtt
     9181 gcagaaatgc agaaacaata taagcatctg tttgcccatg gtgagtatat taaaggcata
     9241 acttcattaa ttaagtacaa gcttaagaaa taaattactc ttatgattac aagtatacgc
     9301 tatcgcggct tctcatttta ttacaaagat aatgataata aatataaaga aatctttgat
     9361 gaaatactgg cctataattt taaaacagta aaagtattac gtaatattaa tgataccaaa
     9421 gtatcattga ttgatacgaa atattgtcgc tatgttttta aagtctttgc cccgaaaacg
     9481 aaaaggaatg aacgtttttt aaaatctttt gtaaaaggtg actactatca gaatttgatc
     9541 gttgaaaccg atcgtgtcag aagtgcaggt cttacttttc ctaatgattt ttattttctg
     9601 gctgagcgta aaattttcaa ctatgccagt gtttttatca tgcttatcga gtatgtcgag
     9661 ggcgtagagc tcaatgatat gccgatcatt ccagaagatg ttaaagcaga aattaaggct
     9721 tcaatggaaa aattgcatgc cttaaatatg ctatcgggcg acccacaccg gggtaacttt
     9781 atcgttagta aagatggtgt caggataatt gacctatccg gtaaaagctg tacggcagag
     9841 cgtaaagctc gtgatcgtct ggccatggaa cgtcacctgg gtattgccaa cgaaataaaa
     9901 gattatggct actattcagt aatctacaga accaaactac gcaaatttat taaaaaatta
     9961 aaaggcaaag cgtaaaccac acagtcaaaa cggaaccaac atggatttat tagctgagag
    10021 tattactgaa gtcgctgtct ctggggaaat tgctaacacc gatcgtgtgt taaatatcgc
    10081 ttacggtatt gaccgtaact ttttatttgg tgcggcagta tctatgcaat cagttgttat
    10141 gcataatccg gaccttgcgg ttaagtttca tctctttacc gactacattg atgaagatta
    10201 tctacaacgt gttaatgctt ttaccagcaa aaatgctaac gttgaagtaa gaatttataa
    10261 agtctccagt gcctttattg atatcttccc cagcctgaaa cagtggtctt atgcaacatt
    10321 cttccgttta gttgcgttcc agtatctgag tgaaactatt gaaaatctgt tatatatcga
    10381 tgctgatgtc atctgtaaag gctcattagc tggattgctt gatattaatt ttgatgggga
    10441 taagtttgca gctgttatta aagatgtgcc ttttatgcag gaaaaaccag cgaagcgtct
    10501 ggctatagag ggacttccag ggaattattt caacgccggt gtagtatatc tgcagcttga
    10561 agcatgggcg aaaaatgatt ttatgaataa agccattgct atgctggcaa gtgacccgca
    10621 gcacacgaag tataaatgcc ttgatcagga tattttaaat attctgttct ttggtcattg
    10681 tatttttatt agcggcgatt atgattgctt ttatggcatc gattatgagt taaaaaataa
    10741 aagcgatgaa gattataaaa agaccattac cgatgatact aagctgattc attatgttgg
    10801 cgtaacgaag ccctggaacg actggacgaa ttatccctgc cagaagtatt ttaatgaggc
    10861 ttatcaggct tcttgctgga atgatgtggc gtttattcca gccacgaatg aaaagcagta
    10921 tcaagtgaaa tatcaacatg caaagaaaaa tggtgatacg tttaacgctt ttatttactt
    10981 tattaaattt aaattaaata agtataaaag aaaactattt ggataataat aaagatcatg
    11041 gatgtctgag ttacaataat aattaaggat gagtactatc atattttatt agttcaggca
    11101 agagaacacg atgagcaatg attacccttt agtatccata ataataccga cgtataattc
    11161 atctgattac attactgaaa ctctaacgaa attagaaaaa caaacttacc caaattttga
    11221 aattgttatt gttaatgatg gttctaaaga taacacatca aacgttttga gagagtatgg
    11281 gttaacccac tctcgattaa ttattatcaa taaagaaaat ggcggtgttt cgtctgccag
    11341 gaatacaggt atccgcaagg cgcaaggaca gtttatatgt tttatggatg atgatgatga
    11401 gatagatcct aactatctgc tgaagatgta taccagacaa catgagacgg gaggggatgc
    11461 gatttattgt gggctttatg gccattatat aaaagatggt gttacttact cacctataaa
    11521 tacagagttt aatgaaggat ctttactttt cgactttttt tataaaaagg ttagattcca
    11581 tatagggtgc ttgtttataa gaaaacaact tctggaagag aataatcttt tttttgatga
    11641 agatttacga ctaggagaag atctggattt tatctatcga ctgctaatta catgcgatat
    11701 gtatgcggtt ccatattata tgtataagca taactataga gaaaattcct taatgaactc
    11761 atgtagaacc atcactcatt atcgacatga gtcatttgcg cacgaaagaa tctactcttc
    11821 tgtgatgcag ttatacaaag gtaacaggaa agaagaaatt catacattat tgagtcaaaa
    11881 tagagcttat cataaaactc gttatttatg gaatgttcta cttaatggtg attttgaact
    11941 attaaatcaa ttagttgaaa gcaatgaaaa agaattgaat gattgtaatc tttctggcaa
    12001 gagagataaa agacgagcaa aaatattagc atcaaaaaat tatattctct ggaggatggt
    12061 aagactggta aataggaaaa agaataaacg ttagatcttg cattaaataa tccgtcctcg
    12121 tagcataggt tgaaactata tgtcattttg ttggaatgaa attaattctg gtataaagtc
    12181 tttaattctc atattatgta ttttttcttt gatgactttg tctttatggg atgatgttgc
    12241 aacaaagttt cttcatgcag ctggaattat atctgcattg tattttctaa taacaccaaa
    12301 aaaaacaata actaacaatc ctactttgtt aattttcatc tcattatgtc ttttgggaat
    12361 cataaatatc atctggtatt cacattataa agtttcaggc tctgtttata ccaatgcata
    12421 tcgtggccca atggaaactg ggaaaattgc cttgtgtagt gcttttattt tcttggttct
    12481 ttttgctaaa aatgagttga gaacaaaaat aaaatttggg aaactaattc tgttcgcatc
    12541 cctggcaacg cagttacttt tttttgcgca tgccatgtgg caacatttct atttaaacgt
    12601 tgaccgtgtt gcattatcag cttcccacgc tacaacagca ggctacatca tccttttccc
    12661 ttctttactg gcatcaattc tcattttaaa atccgacttt agacataaaa caacattata
    12721 tacaattaac ttcatgctta gcttatgtgc tgtcatagta actgaaacgc gtgcagccat
    12781 attagtgttt ccattctttg cgttactatt aatcgtaatg gatagttata ttaataagcg
    12841 aattaattat aagttatatt gttttattgc gattgcatta ttagcaggtg tattttcttt
    12901 taaagataca ttgcttatga gaatgaataa cttaaataac gatttagtta attattcgca
    12961 tgataacacc agaacttcag tcggtgcccg tctggcaatg tatgaagttg gcttaaaaac
    13021 atattctcca ataggacaat cactggaaaa acgagcggaa aaaatacatg aactagaaga
    13081 aaaagagcct agattgagtg gcgctttacc ctatatagat tctcatttgc ataacgatct
    13141 catagatacg ttatcaacgc gtggtattcc tggagttgta ttaacaattt tagccttttc
    13201 agcaatactc atatatgcct taagaactgc taaagaacct tatattttaa tcttgctttt
    13261 ttcactactg gtagtaggcc taagtgatgt aatactcttt tctaaaccgg ttccgactgc
    13321 tgtgtttgtc accataatat tgctttgtgc ttattttaaa gcacaatcag accaatgttt
    13381 attagataaa taatgaatat gtgaaataaa atcagcaagg aggctgattt tatttcaaaa
    13441 ttatctatta aggggttgta atttaaatca tagcaacgtt ttcttcaatg aatcgcttaa
    13501 cagcatttgc tgtcaattga gacaactcat tccccggagc cctacaaacc atctgattct
    13561 tcccatatcc accaattaat cccggatcgg ttggtccata aaccgtgata ttgggtctat
    13621 ccagtgccgc cgttaaatgg cttaaccccg tatccaccga cactacaaat ttagccccgg
    13681 ccagcacgcg ggcaacgcct tccagactca tcttcggcaa tacttcaaca taagcaaatc
    13741 cttccgccag tcgtttcgcc cgctcttcct catgcggcgc gccccacgga agtttaatcc
    13801 gtattcctga atcagccagt aaaccaatca attctcgcca gtgttcttcc ggccagtgtt
    13861 tatcatcacg ggtcgtcgca tgaagaaata cggcatattc gccagcatct gtaggcaggt
    13921 tcgtcaggaa atgctgtgca atagcataat cgccctgggt ttgcggtttg ctatagccca
    13981 gacttttggc aaacagttcg cgggtgcgtt ctacggcgtg ctgctgtttt gcaatatgat
    14041 gcttacgatt gtaaaacaga ctggctaaag gttcgcgagc ggtttgccag tccatgccat
    14101 gctttacgcc atgcgccaga cgcgtcacca gcgccgcgct ttttaccagc ccctgagcgt
    14161 cgataactgc gtcatagttc tctgcttgta gcgcttcacg aaacgctttg cgttccgctt
    14221 ttatgggggc cgagaaccag gctttacgcc agcgacgtat tgccacagga ataactcgct
    14281 caacggcggc gtgccaggaa ggaatctgtg cgaacccttc ttccaccacc cagtcaaact
    14341 taatccctgg gattgcctgc tgggcatcag tgagtgcggg caacgtatgg agaacatcgc
    14401 ccatcgacga tgttttaacg atcaaaaccc gcatccgtca ggcttcctct tgtaacaata
    14461 gcgcgttgag ttcttccagt acgcgctggg gagtaatgtc gattaagctc tggtgataac
    14521 cctccgcagc gtcaccttta cgcactttgt gatagccggt aatcaggcgg atcacgcgcg
    14581 ctttatggga tagcggcggt gtgaagtccg ggctactcgg accatacagg gcaaccagcg
    14641 gacgattgag cgccgccgca acgtgcatca ggccagaatc gttagtgaca atggctttac
    14701 aggctgcaat caggataacc gcttgatcaa gctgtgtttc ccccgccagg ttccgacacc
    14761 atgcctgctg ctcggtattc aaagcggcaa gaatctcatt gcccgcttca tgatctttcg
    14821 ccgagccaaa cagaaccacc tgataacctt catcaatcag ctgctttgcc agttccgcat
    14881 agtggtagtg tggccagcgt tttgccggac caaactccgc acccgggcaa aaaccaatca
    14941 tcggacgttc tgatgaaagc gaaaattgat tacaggtata tgatttttca ccttcgctca
    15001 cctgcaattg cggccataac aacggttttg gtagatcttg tgcggtacgc atcacgcctt
    15061 tgtcataggc cagcgcgaca tagcgttcca ccattagcgg ccaggcttct ttatcgagca
    15121 cgcgtaaatc gttgagtaaa ccgtagcgca tctcgccgcg ccagccggtg cgatgaggaa
    15181 tacccgcgaa gaaaggcact aatgcagatt tgaaggagtt gggtaagacg taggcgcggt
    15241 cgtagcgctt ttcacgcagg ctatgaccca gtttgcggcg ttcgccgatt tccagcgctc
    15301 cgtgaccgag aggcatagga atagcttcgt taacttccgg catccgcgat aataatggac
    15361 ggcaccatgc cggtgccatc acatcgatta tcgcctgggg atagcgcgcc tggagcgtgc
    15421 gatagagact ttgcgacatc atcatgtcgc caacccaaga cgggccgatc accagtattt
    15481 tcatgcagag ctcttatgcg tcgcgattca gccaggccat gtattccgtt acaccttcag
    15541 caacggtttt gaacggtttg tcgtaacccg ccgcgcgcag atttgtcaga tctgcctgag
    15601 tgaacgcctg gtagcggcct ttcagcttat ccgggaacgg aatgtattcg atctggcctt
    15661 tcttgtgata agccagcgta gcatcagcta cagcctggaa ggattccgca cgaccagtac
    15721 cgaggttgaa gatgccggaa acgccatttt ccaggaacca cagattcaca tcagccacgt
    15781 cgcccacata gacgaagtcg cgtttgaagt tctcgctacc ttcaaacagc ttcggtgatt
    15841 caccgttgtt aagctgggtg ttgagatgga aagcgacgct cgccatgctg cctttatggc
    15901 cttcacgcgg tccataaacg ttgaaatagc ggaagccaac aatctgcgag ttagcttcag
    15961 gcaggatttg gcgaacatat tcatcaaaca ggaattttga gtaaccgtag acgttcaacg
    16021 gtttttcgta ctcgcgggat tcaataaagt cggaggtgcg tccgccgtag gtggctgcgg
    16081 aagaggcata caggaacggg atttcgcgct ccagacagta gtgcagcagc tctttggagt
    16141 attgatagtt gttatccatc atatacttgc catcccactc ggtggtggaa gagcacgcgc
    16201 cttcgtggaa aatcgcttcg acatcgccga actcttcgcc agccataatc tggatcagga
    16261 agtcttcctt atccatatag tccgcgatat ccagatccac caggttcaca aacttggtgc
    16321 cgtctttcag gttgtccacc accagaatat cggtgatgcc tttatcattc agggccttaa
    16381 cgatgttgct gccgataaag cccgcgccgc cggtaacgat gatcataact gtaaccttcg
    16441 aattatggag tcagagacaa tctcagacac gaatacttct atcatatcac tacacggcgt
    16501 tggcttcagc cattcgccaa aagtgagatt gatggacgtt atttatcctg aattttgcag
    16561 aagtgttaac gcgtcatctc gtcgcgacct ataagtttgg gtaatatgtg ctggaatttg
    16621 ccctgtctgg agaatcgcaa tgcgtggaga attttatcag cagttaacca acgatctgga
    16681 aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt attacgtctg cgcagcaagc
    16741 agatatcact gtggctgatg gaagccacgt cattaacttt tgtgccaata actatctcgg
    16801 gctggcgaat tatccggatc tgattgcggc ggcaaaggcg ggaatggatt ctcacggttt
    16861 cggcatggct tcggtgcgtt ttatttgcgg cactcaggac agccataaag agcttgaaca
    16921 aaaactggcg gcattcctgg ggatggaaga tgcgattctc tactcttcct gctttgatgc
    16981 taacggtggc ctgtttgaaa cgctgctggg cgcggaagac gccattatct ccgacgcact
    17041 gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa gctaaacgct atcgctatgc
    17101 caacaacgat atgcaggagc tggaagcacg tctgaaagaa gcgcgtgaag ccggtgcgcg
    17161 tcatgtgctg attgccaccg atggtgtgtt ctcgatggac ggcgtgattg ccaacctgaa
    17221 gggcgtttgc gatctcgcag ataaatatga tgccctggtg atggtagacg actcccacgc
    17281 ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa tactgcgatg tgatgggccg
    17341 ggtcgatatt atcaccggca cgctcggtaa agcgctgggc ggggcttctg gtggttatac
    17401 cgcggcgcgc aaagaagtgg ttgagtggct gcgccagcgt tctcgtccgt acctgttttc
    17461 caactcgctg gcaccggcca ttgttgccgc atccatcaaa gtactggaga tggtcgaagc
    17521 gggtagcgag ctgcgtgacc gtctgtgggc gaacgcgcgt cagttccgtg agcaaatgtc
    17581 ggcggcgggc tttaccctgg cgggagccga tcacgccatt attccggtca tgcttggtga
    17641 tgcggtagtg gcgcagaaat ttgcccgtga gctgcaaaaa gagggcattt acgtcaccgg
    17701 tttcttctat ccggtcgttc cgaaaggtca ggcgcgtatt cgtacccaga tgtctgcggc
    17761 gcatacccct gagcaaatta cgcgtgcagt agaagcattc acgcgtattg gtaaacaact
    17821 gggcgttatt gcctgaggat gtgagatgaa agcgttatcc aaactgaaag cggaagaggg
    17881 catctggatg accgacgttc ctgtaccgga actcgggcat aacgatctgc tgattaaaat
    17941 ccgtaaaaca gtcatctgcg ggactgacgt tcacatctat aactgggatg agtggtcgca
    18001 aaaaaccatc ccggtgccga tggtcgtggg ccatgaatat gtcggtgaag tggtaggtat
    18061 tggtcaggaa gtgaagggct tcaagattgg cgatcgcgtt tctggcgaag gccatatcac
    18121 ctgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat ctgtgccgca acacgatcgg
    18181 cgtcggcgtt aaccgcccgg gctgcttcgc cgaatatctg gtgattccgg cgttcaacgc
    18241 cttcaaaatt cccgacaata tctccgacga cctggcttcc atttttgatc ccttcggtaa
    18301 cgccgtgcat accgcgctgt cgttcgatct ggttggcgag gatgtgctgg tctctggtgc
    18361 aggcccgatt ggtattatgg cagcggcggt ggcgaaacac gttggtgcac gcaatgtggt
    18421 gatcactgat gttaacgaat atcgtctcga actggcgcgc aaaatgggta tcacccgtgc
    18481 ggtcaacgtc gctaaagaaa accttaatga tgtgatggca gaactgggca tggccgaagg
    18541 ctttgatatc ggtctggaaa tgtccggtgc gccgccagcg tttcgtacca tgctcgacac
    18601 catgaaccac ggcggtcgca ttgcgatgct gggtattcca ccgtccgata tgtctatcga
    18661 ctggaccaaa gtaatcttta aaggcttgtt cattaaaggt atttacggtc gtgagatgtt
    18721 tgaaacctgg tacaagatgg cggcgctgat tcagtcaggt ctggatctct cgccgatcat
    18781 tacccatcgt ttctctatcg atgatttcca gaagggcttt gacgctatgc gttcgggcca
    18841 gtccgggaaa gtaattctga gctgggatta acacgaacaa gggctggtat tccagccctt
    18901 ttgcctgagg ataatccgtt aaatatgtaa aatcctgtca gtgtaataaa gatatcgtaa
    18961 ttgcgctgat ctcttatata gctgctctca ttatctctct accctgaagt gactctctca
    19021 cctgtaaaaa taatatctca caggcttaat agtttcttaa tacaaagcct gtaaaacgtc
    19081 aggataactt cagaggtcgt cggtaattta tgatgaacag caccaataaa cttagtgtta
    19141 ttattccgtt atataatgcg ggcgatgatt tccgcacttg tatggaatct ttaattacgc
    19201 aaacctggac tgctctggaa atcattatta ttaacgatgg ttcaacggat aattctgttg
    19261 aaatagcaaa gcattacgca gaaaactatc cgcacgttcg tttgttgcat caggcgaatg
    19321 ctggcgcatc ggtggcgcgt aatcgtggga ttgaagtggc aacgggcaaa tatgtcgctt
    19381 ttgtcgatgc tgacgatgaa gtctatccca ccatgtacga aacgctgatg accatggcgt
    19441 tagaggacga cctcgacgtg gcgcagtgca acgctgactg gtgttttcgt gaaacgggag
    19501 aaacctggaa atccatcccc agcgatcgcc ttcgctcaac cggcgtatta accggcccgg
    19561 actggctgcg gatggggctt tcttcgcgcc gttggactca cgttgtctgg atgggggttt
    19621 atcgccgcga tgttattgtt aaaaataaca ttaaatttat tgccggatta catcatcagg
    19681 atattgtctg gacaacagaa tttatgttta atgcgctgcg tgcgcgatat accgagcaat
    19741 cgttatataa atattatctg cataatacgt cagtgagtcg gttgcataga caagggaaca
    19801 aaaaccttaa ttatcaacgt cactatatta agattactcg tctgctagag aaattaaatc
    19861 gaaattatgc cgacaaaatt acgatttatc cggaatttca tcagcaaata acttacgaag
    19921 cattgcgtgt ttgccatgcg gtgcgcaaag agccggatat tattacccgc caacggatga
    19981 ttgccgagat atttacttcc ggtat
//
LOCUS       DAJTVJ010000078        19407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000078 DAJTVJ010000000
VERSION     DAJTVJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.078"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..245
                     /locus_tag="OO448_002699"
     CDS             <1..245
                     /locus_tag="OO448_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR6337872.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="OO448_002700"
     CDS             complement(296..1063)
                     /locus_tag="OO448_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6337873.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="OO448_002701"
     CDS             1259..2167
                     /locus_tag="OO448_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6337874.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="OO448_002702"
     CDS             2164..3426
                     /locus_tag="OO448_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6337875.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="OO448_002703"
     CDS             3423..4061
                     /locus_tag="OO448_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6337876.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="OO448_002704"
     CDS             4066..4842
                     /locus_tag="OO448_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6337877.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="OO448_002705"
     CDS             4931..6295
                     /locus_tag="OO448_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR6337878.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADTGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OO448_002706"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="OO448_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6337879.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OO448_002707"
     CDS             complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="OO448_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6337880.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OO448_002708"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="OO448_002708"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6337881.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OO448_002709"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="OO448_002709"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6337882.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OO448_002710"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="OO448_002710"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6337883.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OO448_002711"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="OO448_002711"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6337884.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OO448_002712"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="OO448_002712"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6337885.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OO448_002713"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="OO448_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6337886.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="OO448_002714"
     CDS             complement(12844..13167)
                     /locus_tag="OO448_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6337887.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OO448_002715"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="OO448_002715"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6337888.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OO448_002716"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="OO448_002716"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6337889.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OO448_002717"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="OO448_002717"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6337890.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="OO448_002718"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="OO448_002718"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6337891.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OO448_002719"
     CDS             complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="OO448_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR6337892.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OO448_002720"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="OO448_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OO448_002721"
     CDS             complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="OO448_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6337893.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4592 a         5232 c         4847 g         4736 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acaccggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagc
//
LOCUS       DAJTVJ010000079        18843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000079 DAJTVJ010000000
VERSION     DAJTVJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.079"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="OO448_002722"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="OO448_002722"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6337894.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2587)
                     /gene="hupB"
                     /locus_tag="OO448_002723"
     CDS             complement(2315..2587)
                     /gene="hupB"
                     /locus_tag="OO448_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6337895.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="OO448_002724"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="OO448_002724"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6337896.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="OO448_002725"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="OO448_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6337897.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="OO448_002726"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="OO448_002726"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6337898.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="OO448_002727"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="OO448_002727"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6337899.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="OO448_002728"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="OO448_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6337900.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="OO448_002729"
     CDS             9871..10449
                     /locus_tag="OO448_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6337901.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="OO448_002730"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="OO448_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6337902.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12429..13376
                     /gene="cyoA"
                     /locus_tag="OO448_002731"
     CDS             12429..13376
                     /gene="cyoA"
                     /locus_tag="OO448_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR6337903.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13398..15389
                     /gene="cyoB"
                     /locus_tag="OO448_002732"
     CDS             13398..15389
                     /gene="cyoB"
                     /locus_tag="OO448_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR6337904.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15379..15993
                     /locus_tag="OO448_002733"
     CDS             15379..15993
                     /locus_tag="OO448_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6337905.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15993..16322
                     /locus_tag="OO448_002734"
     CDS             15993..16322
                     /locus_tag="OO448_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6337906.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16334..17224
                     /gene="cyoE"
                     /locus_tag="OO448_002735"
     CDS             16334..17224
                     /gene="cyoE"
                     /locus_tag="OO448_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6337907.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17409..17720
                     /locus_tag="OO448_002736"
     CDS             17409..17720
                     /locus_tag="OO448_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6337908.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17704..18246
                     /locus_tag="OO448_002737"
     CDS             17704..18246
                     /locus_tag="OO448_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6337909.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18302..>18843)
                     /locus_tag="OO448_002738"
     CDS             complement(18302..>18843)
                     /locus_tag="OO448_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6337910.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4217 a         4953 c         4580 g         5093 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttatta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagtt cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 cttgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctgaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcac tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttccccccac ccaattgtca ccataatgat aattatttgt
    12181 taaacaattg ttttattcaa gattggttat accaattgcc cgtccaaact ccttctgcac
    12241 tcccctttcg ttataacgcc cttttgcaac agcttcttaa aatcaacttg atatgttttg
    12301 caacatatgt aacctgacag ccaaatccaa gtaacaggaa tttaatcatg tttacagtaa
    12361 tgtaacctcc ccgtaaaatg cccacacact ttaaacgcca ccagatcccg tggaattgag
    12421 gtcgttaaat gagactcagg aaatacaata aaagtttggg atggttgtca ttatttgcag
    12481 gcactgtatt gctcagtggc tgtaattctg cgctgttaga tcccaaagga cagattggtc
    12541 tggagcaacg ttcactgata ctgacggcat ttggcctgat gttgattgtc gttattcccg
    12601 caatcttgat ggctgttggt ttcgcctgga agtatcgtgc gagcaataaa gatgctaagt
    12661 acagcccgaa ctggtcacac tccaataaag tggaagctgt ggtctggacg gtacctatct
    12721 taatcatcat cttccttgcg gtactgacct ggaaaaccac tcacgctctt gagcctagca
    12781 agccgctggc acacgacgag aagcccatta ccatcgaagt ggtttccatg gactggaaat
    12841 ggttcttcat ctacccggaa cagggcattg caaccgtgaa tgaaatcgct ttcccggcga
    12901 acactccggt gtacttcaaa gtgacctcca actccgtgat gaactccttc ttcattccgc
    12961 gtctgggtag ccagatttat gccatggccg gtatgcagac tcgcctgcat ctgatcgcca
    13021 acgaacccgg tacttatgac ggtatctccg ccagctacag cgggccgggc ttctcaggca
    13081 tgaagttcaa agctattgca acaccggatc gcgccgaatt cgaccaatgg gtcgcaaaag
    13141 cgaaacagtc gccgaacacc atgtctgaca tggctgcgtt cgaaaaactg gccgcgccta
    13201 gcgaatacaa ccaggtggaa tatttctcca acgtgaaacc agacttgttt gccgatgtga
    13261 ttaacaagtt tatggctcac ggtaagagca tggacatgac ccagccagaa ggtgagcaca
    13321 gtgcacacga aggtatggaa ggcatggaca tgagccacgc ggaatccgcc cattaaaggg
    13381 gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg ttccatgaac
    13441 ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc gttggcctga
    13501 tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc gtcgaccata
    13561 aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt ggttttgctg
    13621 acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggcgaagcg ggcttcctgc
    13681 cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc ttcttcgtgg
    13741 cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc ggcgcgcgtg
    13801 acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt ggtgtgattc
    13861 tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg gcctatccac
    13921 cgctatcggg aatagagtac agtccgggag tcggtgtcga ttactggata tggagtctcc
    13981 agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc attctgaaga
    14041 tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctgggca tcactgtgcg
    14101 cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg ttgttgaccc
    14161 tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac atgatgatgt
    14221 acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc ctgcctgttt
    14281 tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt ggttatacct
    14341 cgctggtatg ggcaaccgtc tgtatcaccg tgctgtcgtt catcgtttgg ctgcaccact
    14401 tctttacgat gggtgcgggc gcgaacgtaa acgccttctt tggtatcacc acaatgatta
    14461 tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat cagggccgca
    14521 tcgtgttcca ttctgcgatg ctgtggacca tcggttttat cgtcactttc tcggtgggcg
    14581 ggatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat aacagcctgt
    14641 tcctgattgc gcacttccat aacgttatca tcggcggcgt ggtcttcggc tgcttcgcag
    14701 ggatgaccta ctggtggcct aaagcgttcg gtttcaaact gaatgaaacc tggggtaaac
    14761 gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg tatgcgctgg
    14821 gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc cacaccatgc
    14881 tgatgattgc agctagcggt gcagtactga ttgcgctggg tattctctgc ctcgttattc
    14941 agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc gacccgtggg
    15001 gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac tttgccgttg
    15061 tgccgcacgt tcacgagcgt gatgcattct gggaaatgaa agagaaaggc gaagcgtata
    15121 aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca ggtatcgtca
    15181 ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg tggctggcga
    15241 ttgttggctt cgcaggcatg atcatcacct ggatcgtgaa aagcttcgac gaggacgtgg
    15301 attactacgt gccggtggca gaaatcgaaa aactggaaaa ccagcatttc gatgagatta
    15361 ctaaggcagg gctgaaaaat ggcaactgat actttgacgc acgcgactgc ccacgcgcac
    15421 gaacacgggc accacgatgc aggcggaacc aaaatcttcg gattttggat ctacctgatg
    15481 agcgactgca ttctgttctc aatcttgttt gctacctatg ccgttctggt gaacggcacc
    15541 gcaggcggcc cgacaggtaa ggacattttc gaactgccgt tcgttctggt tgaaactttc
    15601 ttgctgttgt tcagctccat cacctacggc atggcggcta tcgccatgta caaaaacaac
    15661 aaaagccagg ttatctcctg gctggcgttg acctggttgt ttggtgccgg atttatcggg
    15721 atggaaatct acgaattcca tcacctgatt gttaacggca tgggtccgga tcgcagcggc
    15781 ttcctgtcag cgttctttgc gctggtcggc acgcacggtc tgcacgtcac ttccggtctt
    15841 atctggatgg cggtgctgat ggtacaaatc gctcgtcgcg gcctgaccag cactaaccgt
    15901 acccgcatca tgtgcctgag cctgttctgg cacttcctgg atgtggtttg gatctgtgtg
    15961 ttcactgttg tttatctgat gggggcgatg taatgagtca ttctaccgat cacagcggcg
    16021 cgtcccatgg cagcgtaaaa acctacatga caggctttat cctgtcgatc attctgacgg
    16081 tgattccgtt ctggatggtg atgacaggag ctgcctctcc ggccgtaatt ctgggaacaa
    16141 tcctggcaat ggcagtggta caggttctgg tgcatctggt gtgcttcctg cacatgaata
    16201 ccaaatcaga tgaaggctgg aacatgacgg cgtttgtctt caccgtgcta atcatcgcta
    16261 tcctggttgt aggctccatc tggattatgt ggaacctcaa ctacaacatg atgatgcact
    16321 aagagcggcg gttatgatgt ttaagcaata cctgcaagta acgaaaccag gcatcatctt
    16381 tggcaacctg atctcggtga ttgggggatt cctgctggcc tcaaagggca gcattgatta
    16441 tcccctgttt atctacacgc tggttggggt gtcactggtt gtggcgtcgg gttgtgtgtt
    16501 taacaactac atcgacaggg atatcgacag aaagatggaa aggacgaaga atcgggtgct
    16561 ggttaaaggc ctgatctctc ctgctgtctc gctggtgtac gccaccttgc taggctttgc
    16621 tggctttatg ctgctgtggt ttggcgcgaa tccgctggcc tgctggctgg gggtgatggg
    16681 ctttgtggtt tatgtcggcg tttatagcct gtacatgaaa cgccactcgg tctacggcac
    16741 attgattggt tcgctctccg gcgctgcgcc gccggtgatt ggctactgtg cagtaaccgg
    16801 cgagttcgat agcggcgcag cgatcctgct ggctatcttc agcctgtggc agatgcctca
    16861 ctcctatgcc atcgccattt tccgctttaa ggattatcag gcggcaaaca ttccggtatt
    16921 gccagtggta aaaggcattt cggtggcgaa gaatcacatc acgctgtata tcatcgcctt
    16981 tgccgttgcc acgctgatgc tctctcttgg cggttacgct gggtataaat atctggtggt
    17041 cgccgcggcg gtgagcgtct ggtggttagg tatggccctg cgcggttata aagttgctga
    17101 tgacaggatc tgggcgcgca agctgttcgg cttctctatc atcgccatca cagccctctc
    17161 ggtgatgatg tccgttgatt ttatggtgcc ggactcgcat acgctgctgg ctgctgtttg
    17221 gtaacacaac ctctctatta aaaaggtgct acggcacctt ttttattgtc tgagatcaga
    17281 gatacggcta ataaccgtat gtcttgacag agtgagcaga tgaacataaa ttaatcatac
    17341 ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag ttaatcaaag
    17401 aggtcataat gcctggaaaa acagccactc tacccgatgt cgataaaacg ctgaaaaatg
    17461 cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa gctgcgcaag
    17521 ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc gccgaaagtg
    17581 tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg atgaaccaac
    17641 tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa aggaaaaaca
    17701 gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg taattatcag
    17761 tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaaccctgt tatcgataaa
    17821 tttgtacgcg catcgctaaa gaaaagtgtg cgtaatagcg actgtgcggc taaagcactt
    17881 attgacagac aaagtggtga actgatcggc atctgtactt ttacggcata ttcgctggaa
    17941 aaacaacgcg tttctggcgt ccttcagggt tcacaacctt cagaaattgg tgttgtcaga
    18001 ttagtcatgt tgggggtagc acggaagtat caaaagcggg gctttggtca ggacctacta
    18061 tgtgattttt ttgaacatgt aaaaataatt caccaggcat taccaattaa aggggtttat
    18121 cttgatgctg accctgccgc cattaatttt tatgctcgtc tcggctttgt tcagctttca
    18181 gcgacaccaa atgcttttgg tgctgtacct atgtttttgg cgattcagca tattctcgcg
    18241 gcttagatta ttatcgccac ccgccagggc aagttttgcc ttagccgggg gctgagcagc
    18301 gttaccactt ttgttcaatt gcccccgctt tcagcaactc cacatccttg ccgttaatgg
    18361 ttgccgttgc ggcaacgatt gtggtcctta cagttgcaca agattttcgc tcaaattccg
    18421 gtttataaag aataagagac gaattactgg taaatgaacc aaataggtaa tcagtaaagc
    18481 taatattcag ccggtttaaa ccgaaaggcg cgaagccaac cgtttggtta atctccttac
    18541 cggatgggcg acgaatgctg aatgtcacgc gaagattggc cacttcgcct aatttattgg
    18601 tggtggcgaa gaggctatcc atgcaggaaa aacctttatc taaaaaatta tctcgcatcc
    18661 cagaacgggt aaccgtaatc acgggtaacg tatcgtcaac cgtttttctg tcaatcatgt
    18721 cttttcgaca agtagccaga aagtcttccg ggccaccata aatgtggaca cctttatcaa
    18781 gatattcctt cgctttctta caatctacct caacatactg accattggcg tacataatgc
    18841 cca
//
LOCUS       DAJTVJ010000080        18513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000080 DAJTVJ010000000
VERSION     DAJTVJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.080"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1710
                     /locus_tag="OO448_002739"
     CDS             <1..1710
                     /locus_tag="OO448_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6337911.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="OO448_002740"
     CDS             1711..3360
                     /locus_tag="OO448_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6337912.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="OO448_002741"
     CDS             3365..4141
                     /locus_tag="OO448_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6337913.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OO448_002742"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OO448_002742"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCR6337914.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="OO448_002743"
     CDS             complement(5430..6752)
                     /locus_tag="OO448_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCR6337915.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OO448_002744"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OO448_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCR6337916.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OO448_002745"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OO448_002745"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCR6337917.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OO448_002746"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OO448_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCR6337918.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OO448_002747"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OO448_002747"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCR6337919.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="OO448_002748"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="OO448_002748"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6337920.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OO448_002749"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OO448_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6337921.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OO448_002750"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OO448_002750"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6337922.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4524 a         4534 c         4866 g         4589 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aat
//
LOCUS       DAJTVJ010000081        18505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000081 DAJTVJ010000000
VERSION     DAJTVJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18505
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.081"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            68..280
                     /gene="cspH"
                     /locus_tag="OO448_002751"
     CDS             68..280
                     /gene="cspH"
                     /locus_tag="OO448_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6337923.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            727..1032
                     /locus_tag="OO448_002752"
     CDS             727..1032
                     /locus_tag="OO448_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337924.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1139..1783
                     /gene="gfcB"
                     /locus_tag="OO448_002753"
     CDS             1139..1783
                     /gene="gfcB"
                     /locus_tag="OO448_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR6337925.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1780..2526
                     /locus_tag="OO448_002754"
     CDS             1780..2526
                     /locus_tag="OO448_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6337926.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2526..4622
                     /locus_tag="OO448_002755"
     CDS             2526..4622
                     /locus_tag="OO448_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6337927.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4668..5807
                     /locus_tag="OO448_002756"
     CDS             4668..5807
                     /locus_tag="OO448_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6337928.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5795..6241
                     /gene="etp"
                     /locus_tag="OO448_002757"
                     /gene_synonym="yccY"
     CDS             5795..6241
                     /gene="etp"
                     /locus_tag="OO448_002757"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6337929.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6261..8441
                     /gene="etk"
                     /locus_tag="OO448_002758"
     CDS             6261..8441
                     /gene="etk"
                     /locus_tag="OO448_002758"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR6337930.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8556..9854)
                     /gene="appA"
                     /locus_tag="OO448_002759"
     CDS             complement(8556..9854)
                     /gene="appA"
                     /locus_tag="OO448_002759"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6337931.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9934..10026)
                     /gene="cbdX"
                     /locus_tag="OO448_002760"
     CDS             complement(9934..10026)
                     /gene="cbdX"
                     /locus_tag="OO448_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6337932.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10039..11175)
                     /gene="appB"
                     /locus_tag="OO448_002761"
     CDS             complement(10039..11175)
                     /gene="appB"
                     /locus_tag="OO448_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6337933.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11187..12731)
                     /gene="appC"
                     /locus_tag="OO448_002762"
     CDS             complement(11187..12731)
                     /gene="appC"
                     /locus_tag="OO448_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6337934.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12865..13722)
                     /locus_tag="OO448_002763"
     CDS             complement(12865..13722)
                     /locus_tag="OO448_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6337935.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13779..14117)
                     /gene="hyaE"
                     /locus_tag="OO448_002764"
     CDS             complement(13779..14117)
                     /gene="hyaE"
                     /locus_tag="OO448_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6337936.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14114..14701)
                     /locus_tag="OO448_002765"
     CDS             complement(14114..14701)
                     /locus_tag="OO448_002765"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6337937.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14698..15404)
                     /gene="hyaC"
                     /locus_tag="OO448_002766"
                     /pseudo
     CDS             complement(14698..15404)
                     /gene="hyaC"
                     /locus_tag="OO448_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15423..17216)
                     /gene="hyaB"
                     /locus_tag="OO448_002767"
     CDS             complement(15423..17216)
                     /gene="hyaB"
                     /locus_tag="OO448_002767"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6337938.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17213..18331)
                     /gene="hyaA"
                     /locus_tag="OO448_002768"
     CDS             complement(17213..18331)
                     /gene="hyaA"
                     /locus_tag="OO448_002768"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6337939.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4544 a         4910 c         4779 g         4272 t
ORIGIN      
        1 aaatatcgct ggctttattt tattaacccc gtatagtgca gcgcagtatt ttcagtaagg
       61 aatttgtttg tcccgtaaaa tgacaggaat tgtcaaaacc tttgatcgca aaagcggcaa
      121 aggattcatt atcccctccg acggtcgtaa agaagttcag gtccatattt ccgcattcac
      181 tccccgcgac gcagaagtgc ttataccagg attacgtgtg gaattttgcc gcgtaaatgg
      241 cctgcgagga ccaacagcgg caaatgttta tctctcttaa tatgacgctg cttgtttaaa
      301 gctgactggt tttattggaa tagtgcaata gtcaacatca tacattataa tatcagttac
      361 ttttccacaa agaaactaat ccatttaagc gaatatctct gcacctaata gttataaatg
      421 ccatttaaca gccagttata gtaccacttg aaactatatt accaacaata attaatagct
      481 ttcgtaacaa tttccttatc tttaatttgt attttgctaa gatttatctt atgctcacaa
      541 gtcgtatttc cagaggggat cgttaataat cttatatttc cctctgcgca cctttcctac
      601 ccgcagtatc agtatatagt gcaagcggta tgaggaaagg gagatttcac aaggatgtta
      661 tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat gaataaagga
      721 aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac gacgccagct
      781 gcgtttgctg cctctgaagc aacaaacggg accgaggcaa cgacgggtac tactggcacc
      841 acaacgacca ctaccggcgc aaccacgact gctactacca ctggtggcgt ggctgctggc
      901 gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg ttggtgtggc gacgttggca
      961 gttgttgcag caaatgacag tggcgatggc ggttctcata acacctcaac caccacgtcc
     1021 actacacgct aatactgctt taacgcgtcg aaattaaagt taaatgatac ccggttgcga
     1081 tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag gattaaaggt
     1141 gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc acagccagca
     1201 aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca ccgtagcgga
     1261 tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg aagggcaacg
     1321 aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat cccaggataa
     1381 cgccatgctg gttacccaca atggacgtct tttaaaaacc gtcaaactta ataataatct
     1441 gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa taaaagatgg
     1501 cagccgctgg acgcgcgata ttctctggag tgaagacaac cattttcgct ctgcgaccct
     1561 gagttctact ttttcctttg ctggattaga gacgctgaat attgcgggtc gcaatgtgct
     1621 gtgtaatgtc tggcaggaag aggtgacttc cacgcggcca gaaaaacagt ggcaaaacac
     1681 attctgggtc gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt taggcgcagg
     1741 ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt acagtcgtat
     1801 ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg aacggtgact
     1861 atttatctgc ctggcgaaca acagacactt tccgttggcc ccatggaaaa tgttgtccag
     1921 ttggtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt gctgaccgac
     1981 tctgcggcga aagctaaagc gcttaaagac taccaacatg tcatggcaca gcttgcttcc
     2041 tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg ccagcagtta
     2101 ctcaatctca atatcactgg tcgcttaccg gtcaaactgg accctgattt tgtacgagtg
     2161 gatgagaaca gcaatccccc gttggtcggg gactatacgc tctataccgt acagcgtccg
     2221 gtgactatta ctctgctggg tgcggtttct ggggccgggc aattgccctg gcaggcaggc
     2281 cgtagcgtta ccgattatct gcaagatcac ccccgccttg caggcgcgga caagaataac
     2341 gttatggtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct gtggaataaa
     2401 agacatgtgg aaccaccgcc tggcagccag ctttggttgg gcttttctgc gcatgtactg
     2461 cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca acgtgttcct
     2521 gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgccgtc tccagtgcct
     2581 gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatca gactttggcg
     2641 gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa ttcagcgtca
     2701 actaccggga taacgatcaa taccggttct actccacctc cgtggcgctg ttcccatggc
     2761 tggaaggcac cattcgttat acggatgtgc gcacacgcaa atatagccag tgggaagatt
     2821 tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc ctgtgggaag
     2881 aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt acgggtctgt
     2941 ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc accctcggga
     3001 tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc cgggtgagcg
     3061 ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt agcgatatct
     3121 ttcgtggccc ggcttccatc tttggcggca ttgagtatca aacgccgtgg aatcccctgc
     3181 gtctgaaact cgaatacgat ggcaacaatt accagaatga tttcgctggc aaactgcctc
     3241 aggcaagcca tttcaacgtc ggcgcagttt atcgcgctgc cagctgggca gatctcaacc
     3301 tgagttatga acgcggtaac acgttgatgt ttggcttcac gttacggacc aatttcaacg
     3361 atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg cctgaatctg
     3421 aaggattgca gtacaccaca gtagcgaacc aacttaccgc gctgaagtac aacgcaggtt
     3481 ttgaagcacc ggaaattcag ctgcgcgata agacgctgta tatgtctggt caacaataca
     3541 aataccgtga ttctcgcgaa gcggtcgatc gtgccaaccg gattctggtg aataacctgc
     3601 cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg atggtgacta
     3661 ccgaaaccga cgtagccagc ctgcgcaaac agctggctgg tacagcgcct ggtcaatcag
     3721 agcaacttca acaacaacgt gttgaagcag aagatctttc tgcctttggt cggggctacc
     3781 gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag tcgctgggcg
     3841 gcccggaaga tttctatatg ttccagctgg ggctgatgtc cagtgcccgc tactggttta
     3901 ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac tacgacaagt
     3961 ttaagtcttc gctgttgccc gcggactcta ccctgccccg cgtgcgcacg catatccgtg
     4021 attacgttcg caatgacgtt tatctcaaca acttgcaggc gaactacttt gccgacttag
     4081 gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtac gccggtgtcg
     4141 gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg gacgttaact
     4201 acgtgaagca acgtgactgg gacaacatga tgcgcttcac cgattattcc acgccaactg
     4261 gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg aaacttagcg
     4321 ttgggcaata tctggcaaaa gataaagggg caacgatcga cgtcgccaaa cgctttgaca
     4381 gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat gactacggcg
     4441 aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg accattggac
     4501 ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt ggacaaatgc
     4561 tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg gttggacaat
     4621 aacctgacgt aattcataac attacattga ctgataaata taaaccaatg aaaaagaaca
     4681 tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt acccttgttc
     4741 ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac aatcagtatg
     4801 acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat cagttgcgcg
     4861 ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgca aactatgaat
     4921 atcgtatcgg gattggtgat gtgttgatgg ttacggtctg ggatcacccg gaactaacca
     4981 cccccgcagg gcaataccgt agcgccagtg ataccggcaa ctgggttaac gccgatggtg
     5041 cgattttcta cccctatatt ggtcgcctga aagtggccgg gaaaacactt acccaggtac
     5101 gcaacgagat tacagcgcgt ctcgattcag tcattgaaag cccgcaagtc gatgtcagcg
     5161 tcgcggcgtt ccgatcgcaa aaagcgtacg tcaccggcga agtcagcaaa tcgggtcagc
     5221 agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg ggcggtctga
     5281 ccgcagatgc agactggcgc aacgtggtgc tcacccagaa tggcgtaaag accaaagtga
     5341 acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg cacccgggcg
     5401 atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt gaagtgggta
     5461 aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg ctgggtaacg
     5521 ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt cgtgcaacgc
     5581 aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag gacgcatcgg
     5641 caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat gtcactaccg
     5701 cgccgctggc tcgttggaac cgtgtgattt ctctgctggt accgacgatt tccggggtgc
     5761 atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat ttaactcaat
     5821 cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct tgctgcgtaa
     5881 acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctgataa aacaccctgc
     5941 agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag gacatgccgg
     6001 acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa tggagtcgga
     6061 acatattgct caggttaccg caattgctcc cgaggtccgc gggaagacaa tgctgtttgg
     6121 gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg acgcatttga
     6181 acatgtctac ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc tcagccgata
     6241 atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca ggcagcactc
     6301 aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac cgtaagttta
     6361 ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg ttaagcacac
     6421 caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac gccattctca
     6481 gcggcctgag cgatatgatc cctaactcat cgcccgagtc tgcaccggag atccaactgc
     6541 tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc gacatagttg
     6601 agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa gaaaaaccag
     6661 gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat cagcaactga
     6721 cactcacggt tggggaaaac ggccactata cactggaagg tgaagagttc accgtcaatg
     6781 gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg gacattaagg
     6841 ccaaaccagg aacacagttt gtcctgagcc agcgtaccga actggaagcg attaacgcat
     6901 tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg gaacttacca
     6961 tgactggtga tgatccccag ttgattactc gtattctgaa cagcatcgct aacaactatt
     7021 tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa ttcttacagc
     7081 gccagttacc tgaagtgcgc agcgagctgg accaggcgga agaaaaactc aacgtttatc
     7141 gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt gagcagattg
     7201 tgaacgttga taatcaactc aatgagctga ccttccgcga ggcagagatc tcccagctgt
     7261 ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg ctggagcaag
     7321 aacgcaaacg cctgaataag cgggtatcgg caatgccttc cacccaacag gaagtgttgc
     7381 gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt aaccgccagc
     7441 aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcggattatc gacccggcag
     7501 tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt ggttttattc
     7561 ttggcctgtt tatttctgtg ggtgccgtgc tggcgcgtgc gatgttgcgt cgtggtgtag
     7621 aagccccgga acaactggaa gagcacggca tcagcgttta tgccactatc ccaatgtccg
     7681 agtggctgga taaacgcacc cgtctgcgta agaaaaattt attttctaat cagcagcgcc
     7741 atcgtactaa aaatatcccc ttcctggcgg tggataaccc ggcggattct gctgtggaag
     7801 ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat ggagacggag aataacattc
     7861 tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt tgtcagttca actctggcag
     7921 cggtgatcgc ccagtccgat caaaaagtgt tatttattga tgccgactta cgccgtggtt
     7981 attcgcataa cctgtttacc gtgagtaatg aacatggctt gtcggaatat ctggcaggta
     8041 aagatgagct caacaaagtg atccagcatt ttggcaaagg aggctttgat gtgattactc
     8101 gcggtcaggt gccacctaac ccgtctgaac tgctgatgcg cgatcggatg cgtcaattac
     8161 tggaatgggc gaacgaccat tacgatctgg tgattgtcga tacgccgccg atgctggcgg
     8221 tgagtgatgc cgcggtcgtg gggcgttctg ttggcaccag cctgctggtt gcgcgttttg
     8281 gcttgaacac cgccaaagag gtgagtttgt caatgcagcg tctggaacag gcaggcgtca
     8341 atattaaagg cgctatcctc aatggtgtga ttaaacgcgc cagcaccgct tacagttacg
     8401 gctataacta ttacggttat agttactccg agaaagagta aaactgaaag tggccggatg
     8461 cggcgtgaac gccttttccg gtctacgcat tagacagttc ttcgtttgtc atcagcctca
     8521 gagcattcag gtaactgaat gctctttttt atgcattaca aactgcacgc cggtatgcgt
     8581 gcttcattca cgatttgcgt aaaacctgcc aacgaacaca tgccctgcgc atttcgctct
     8641 tcacatcctg ccagggtcag tttcacctct ccgggcggcg tatttaatga cagcggcgtt
     8701 ttatcacgca tctgctgtaa agtctggaag accagcgaaa cctgaatcca ctggctgtta
     8761 tcgcttagcc gacgccagcg ttcaaacacc agttcaccac ctggcggcgt gttatccggc
     8821 tgaccgggaa gcgtccagtt gagctccagt gcgccgccga gatttgccag attagtatcg
     8881 tgtccggcga taaacagcac tgaagtgggt aatgtcacac catacgcctg tttttgcggt
     8941 ggatggggcg tcaacgctgt cttgatcaaa tctaataacg gggtggcgcg gctgcgggca
     9001 acctctggcg tgcgttgtag caaataaaat tgcgcgttat gcaaacttag caaggtgttc
     9061 cactggtgtg aatcggtgat ccttccccac cccggctccg gcattccctg tgcttgttgc
     9121 aggagaaata tctccgtcag cattgatgcg aggcttaccg caccggttaa tgagacattg
     9181 tcggcgctca ccttgagttc cgatggtaat gcctgcgtta atgaacagct ttcgtcctgt
     9241 ttctcacgtt taaggcacaa gtttgattgc ggaaaattaa gcacccgttc cagttcgcga
     9301 aacgccgttt gccgatgccc ggtaaagtca gcaattgacc ctcctgccct gctgaggatc
     9361 gcgtcagtca cgttcgcgtt atccagttgg caaacgccag tttttagagg attaaataac
     9421 ggatcgggac tggacgtatc agcctgggta tgtacggtta ttgcacagtc aggtgccagc
     9481 ccggcggcga aggcttcgcc tgttttacgg gtacgctcgt cgacatcagc aataatcgcg
     9541 acctgaccag actgcgggca gccctttttc gccagcaatc cgtcggctac cagacgctgg
     9601 cgttggtaat gtccgagata ggcgattagc tcaccaccgc gcggtgtcag ccaacccagt
     9661 tttaccggcc aggttggcca tgcgtctggg gtgacatcct gcatcagttg cgtggccttg
     9721 gttggagcac gcacaccatg acgactgaca atcaccacac tttccagctt cagctccggc
     9781 tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa aaatgggatt
     9841 aagatcgctt tcatcgatat gtttccgctt ttcatatctg acattattga ttgcctgatg
     9901 cgatgctaat gcgccgcatc aggcctacgt tcgttaactt ttcagacgcg ggtccagcca
     9961 taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc aaagtaaata
    10021 ccacattgtt cctgctcctt agtacaactc gttttcgtta cggcggagag tttctgttgt
    10081 catgcgtccc cacattttgt agtagctcca gagagtgtag agcaacacaa tgggcaaaaa
    10141 tatcagcacg attaccaaca taatgctcag cgtcagctga ctggaagtgc tgtcccacaa
    10201 cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca gcgtgatgcc
    10261 tgccgtgaaa atcacgccaa attgcatcaa tgacgccatt aaaaatcccc aacccgggcg
    10321 accacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca gcgggaagat
    10381 ccacaaaacg ggcgattcga cgaaattatt catccaggca ccaggtagca ctgccaccag
    10441 tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa agccatcaat
    10501 accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca gtgcagcgcg
    10561 tttggtggcc agctgtgaac gcagatgaat cacaccaaca gttttcagtt gtaaccagac
    10621 gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag ggaatggcgt
    10681 cagcagttgc cagaagctgc cgagatactc cacgcgtaat tgcggtgtga aggcaaacgg
    10741 cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca ccagactgcc
    10801 gatgaccaga ccggcgtccc acattttacg ccagcgtgca tcggcgattt ttccgcgata
    10861 atcaaaggcc agcgggcgga agaacagtga gcacagcacc aggatcatcg ccacataaaa
    10921 gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc caccagcgag
    10981 gatcaaccag acctggttgc cttcccagtg tgcaccaacg ctgtttatca ctatccggcg
    11041 ttcatcatca ttacgcgcca ccagcggcag cagacagccg atccccatgt caaatccgtc
    11101 ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga agcgcaatgt
    11161 ttcataatca aacatgattt tcccctttac ccctgttgct gcgtcggttg ttcactctgc
    11221 atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc gattaagaac
    11281 agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt ggttaaagcg
    11341 gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg accaaactcg
    11401 gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca gagcgccatt
    11461 ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag cgcaatcagc
    11521 atcaccagta gcggcaggga accacagccc accatgatgc ggaaactcca gaataccggc
    11581 gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt gacatgattc
    11641 atatccggcg catagcggga gagcaacatg ccgtagccca ggtcaccttc cagttcccgg
    11701 aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat taacagccag
    11761 gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattttt cagacccggc
    11821 acggttttat ctaatgagtg agtggcgagg atccctaaca gcgcgggaat tttgatggca
    11881 aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg tgcaggagca
    11941 ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac ttgcgcgact
    12001 tcatacgcag aactgtctcc gagttgtagc gtgccgataa tcgccagagt accgaagacg
    12061 gaaccgatgg caaacgagcg taatgcaaca tcgcgctccc gtccgcgcag taaataccag
    12121 gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac ggtgtgcaca
    12181 aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat ctccatacgt
    12241 agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt ggcattcaat
    12301 atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag caggtgctgg
    12361 tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga ttcgaggaag
    12421 aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac atagttggaa
    12481 tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc cacgccaaga
    12541 gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta gattgttttg
    12601 ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc cagggtaagg
    12661 ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga taaatcaatg
    12721 acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgata atgttagatc
    12781 tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat attcataaga
    12841 ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc actgacatac
    12901 ctcgctaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca ccacttccgg
    12961 tattgggcag atttcataac tttccagtaa aggacctttt aaggtgtccg tacagcgtaa
    13021 atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc caatggtacg
    13081 aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag cctcgctgat
    13141 gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta catgcgccag
    13201 taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg gtaacagcga
    13261 atctgtcggc aagagatttt gcgtggccgc ctgccacaac gccagcggcg cgcagccggc
    13321 ctccagtttg tcctccagca acttttcgcc gcgacgtttg cgcacccgcc ataatccgca
    13381 gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc gcactgacac
    13441 ttccccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga gatttaacag
    13501 atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat gttgtaactc
    13561 gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg ccatactcgg
    13621 ttcatcattc acctgacagc tgatcggcag tggattcatg ctgaaactgt catcgttcgg
    13681 ttgcgttcct ggccccagca gatggaaaaa agtttcgctc atgaggcacg ctcctgttgt
    13741 ggttcgacaa gcccacgcat caggtttatc agttccgctc acgggtgaat accattcagc
    13801 acaccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa gacgccaaag
    13861 cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca tgtgtagccg
    13921 ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc tggcgtgcgt
    13981 ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca gtcgtcaaga
    14041 cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa cgcatcaaat
    14101 ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc aagcgaacgc
    14161 cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca ttagccggtt
    14221 gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct gcgggcagtt
    14281 gctcccgtgc cagttcgctc aggctgccgc cgtagtcgtc gagcattgcg ggttgcagac
    14341 cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc agcacttcgg
    14401 agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga atacgttctc
    14461 cggcataggt tcgcagcgtt ccaggttcca gcccgtagtc aatggcatcg agaatcaaca
    14521 gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg ccatcgacaa
    14581 tctccacata ctcgggccag tggtaatggg catacaaccg ttccgccacc cgcacgccga
    14641 aaccttcatc ggcccacagc aggttgccca gccccattac cacaacgcgt tgctcgctca
    14701 tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt tgaccatggt
    14761 ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga catgaccgat
    14821 cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt ccattgagtt
    14881 gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa tagcgtactg
    14941 gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca tcaggaaata
    15001 gccgaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg gacgttttgc
    15061 cagaacagat accagcggat ttcataccac acgccctgcc accagctttt acgccatacc
    15121 ggcacgataa acagctcgcg ggagtatcga ttgccaacaa aagcccagta gatccgcatc
    15181 agcaagacca cggtaaaaac catcccggcg ctgaagtgaa ttaacctgat gtagcccata
    15241 tagaacagat acgtcgcctc gccgctgacg gaaggtagcg gcttgccgat aaagtatccg
    15301 gtgaccatca acaccgccat gcataacacc gtcagccagt gccagatgcg gactggcgct
    15361 tcaaagacat agtggctgac aacgttgtcg cttttctgtt gcatgatgat tctccttcgc
    15421 tgttaacgca cctgcacgga gatcagctcg ctaccgtcgt cgcccagcac gtgtgttgaa
    15481 caggcgaggc acgggtcaaa gctgtgcaga gtacgcagga tctccagcgg ttgctcgggg
    15541 atcgccattt tggtgttcat cagcgccgct tcataagcgc caatctgccc tttgggatcg
    15601 cgcgggctgg tgttccaggt ggtcggcacc acgcactggt agagatcaat cttgccatcg
    15661 cgaatggcgg cccagtggcc taacgccccg cgcggcgctt cggtaaagcc gacgccacgg
    15721 cactctgtcg gccaggttgc aggttcccat ttttccgtgg aggcggtggc aagattgccg
    15781 tttttcaggt tagtcatcag cttgttgaag aaatattgca acttacctgc ggcccactgt
    15841 gcttcgtgcg cgcggcacaa aatgcggccc aacgttgact ggataccgga aagcggcagg
    15901 ttcagcgccg acatcatgcg atcgaccgac tcaacggtcg cagcatcgcc tttgtgataa
    15961 gcgattaacg tgcgtgccag cggccccact tccatcgcgt taccgcgcca gcgcggcgct
    16021 ttgatccacg agtagcgttc ctgttcattc agctgctgaa tgttggtatc gctgcctttg
    16081 acatcgccgg ggttgtacca cgggtcggta atgccatcga atggatgacg cccgacctga
    16141 tcgttgggat aacgatacca ggcgtggtcg acaaactcct gcacctgctg cggatcaacc
    16201 aaatccactg gcagcacatt gttgaagtcg ccgttaatca ccgcgccgcc aggcatcagc
    16261 agacttttct caccaaagtc gttggcaata tccgggaacg caccgtagct gaggacacat
    16321 ttatcagaaa gacccgtgcc gatttcgctc cacggtttgt tgaactgacc gatggctaag
    16381 gcgtcgggga tcatcacgtt gttaatgaag tccgccgtgc gggtgataat cgactgcacc
    16441 aggttcaggc gttccatatt gaccgccccg accgcgccgc tttcgtcaat gttaatggcg
    16501 caaggcatcc cgccgacaat ccagtttgga tgcgggtttt taccgccaaa gaccgcgtgg
    16561 attttgacaa tttcacgctg gaaatcgaga gcttcgagat agtgggcaaa gcccatcagg
    16621 ttagcttctg gcggcagttt gtactgcggg tgcccccagt agccattgcg gaagatcccc
    16681 aactgcccgc cttcaacaaa ttttttcagg cggttttgta cgtcgaagaa atagccaggg
    16741 gatgatttcg gccaagagga gagactttgc gccagttcgg aggttttccg cgggtcggct
    16801 ttcagcgcat ctaacacatc gatccagtcc atcccggcaa gctgatagaa gtgcaccaga
    16861 tgatcgtggc accagagcgt tgccagcata atgttgcgga tgatattggc gttgtccggc
    16921 actttaatac cgatagcatc ttcgatggcg taaaccgaag ccagggcgtg tacgccagta
    16981 cagacgccgc agatacgttc aacgaacgcc cacgcatcgc gcgggtcgcg cccttgtagg
    17041 atgatctcca gcccgcgaaa catggtgccg caggagacgg cattggtgat cacattctga
    17101 tcgttaatat tcacttcgca gcgcatgtgg ccttcgatgc gcgtaatcgg gtcgaccacc
    17161 aggcggcgtc cggcattatt gatggtgtat ccctgagttt cgtactgagt gctcatgcct
    17221 gtttatcctc attgcctggc tgatgttcgg tttctgtagg ttgctggtta tgacgtctgc
    17281 gctggtcaac ggcgctggcg actgcgtgca caccaacagc cgctgccacc acaccaagcg
    17341 cggttaaacc gacggtatcg gcggtggaat gagtacccat ttgcggaata tcgaccacgc
    17401 ggctgtagaa cgaaccgcaa tcccagaaac cattttccgc acagcccagg cagccgtgac
    17461 cagactggat tgggaaagaa acgccatcat tccagcgtgt ggaggaacag gcgttatagg
    17521 tggtaggccc tttgcagccc attttgtaca ggcagtaacc tttgcgggca gcgtcatcat
    17581 cccaactctg gacgaactct ccggcgtcga agtgggcgcg gcgatagcat ttatcgtgga
    17641 ttcgctgacc atagaacatc agcggacggc ccattctgtc gacatctggc aagcgatcaa
    17701 aggtcaccat gtaagtaatg atggcgctca tcacatccgg gatcggcggg cagccaggta
    17761 ctttgataat gggtttgtcg gtgatgactt tgtcgatagg cgttgcctgc gtcggattgg
    17821 gccacgcggc ctgcacgcag ccccaggacg cgcaggttcc ccaggcgata atcgcgctgg
    17881 ctccggcagc ggcacgtttg agtttctcaa taaacggtcg accgctgctg atacagaaca
    17941 tcccctgctc gcccagcggc ggattacctt ctactgcgag gatatatttg ccattgtatt
    18001 gcgtgatgat gtcttcgaag acttcttccg cctgggttcc ggcggcagcc atcaaagtat
    18061 cgtcataatc gagggaaatc agggaaagga tgacgtcctt cgccagtggg tgagcggagc
    18121 ggataaaaga ttcggtacag caggtgcatt ccagaccgtg gatccatacc accggaatgc
    18181 ggggtttgtt ctccagcgcc caggcaatct ttggtgccat tcccgcgcct aatcccagcg
    18241 acgtggcagc cagactacaa tatttgagaa agctgcgccg ggtaacgccc tgacgccgca
    18301 tggcctggta aaatgtttcc tcgttattca tatcgcacgt ctcttctcct tgcgacaccg
    18361 gcaggacacc gggcgcttat ggtcggcaag cgacgtccct gtagcaaggc aaaccatcgc
    18421 ttcacgtgag atgctgaaaa cgaaagctca tccttctgca ctggcgcacg tcgccagaaa
    18481 gtattgttaa taaagcgtag tgaaa
//
LOCUS       DAJTVJ010000082        18430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000082 DAJTVJ010000000
VERSION     DAJTVJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.082"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(167..577)
                     /locus_tag="OO448_002769"
     CDS             complement(167..577)
                     /locus_tag="OO448_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCR6337940.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(635..868)
                     /locus_tag="OO448_002770"
     CDS             complement(635..868)
                     /locus_tag="OO448_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR6337941.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1257..1802
                     /locus_tag="OO448_002771"
     CDS             1257..1802
                     /locus_tag="OO448_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6337942.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1777..3702
                     /locus_tag="OO448_002772"
     CDS             1777..3702
                     /locus_tag="OO448_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6337943.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3699..3905
                     /locus_tag="OO448_002773"
     CDS             3699..3905
                     /locus_tag="OO448_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR6337944.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3902..5503
                     /locus_tag="OO448_002774"
     CDS             3902..5503
                     /locus_tag="OO448_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6337945.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5484..6803
                     /locus_tag="OO448_002775"
     CDS             5484..6803
                     /locus_tag="OO448_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6337946.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6813..7145
                     /locus_tag="OO448_002776"
     CDS             6813..7145
                     /locus_tag="OO448_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6337947.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7201..8226
                     /locus_tag="OO448_002777"
     CDS             7201..8226
                     /locus_tag="OO448_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6337948.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8268..8666
                     /locus_tag="OO448_002778"
     CDS             8268..8666
                     /locus_tag="OO448_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6337949.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8678..9031
                     /locus_tag="OO448_002779"
     CDS             8678..9031
                     /locus_tag="OO448_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR6337950.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9043..9621
                     /locus_tag="OO448_002780"
     CDS             9043..9621
                     /locus_tag="OO448_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6337951.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9618..10013
                     /locus_tag="OO448_002781"
     CDS             9618..10013
                     /locus_tag="OO448_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6337952.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10021..10761
                     /locus_tag="OO448_002782"
     CDS             10021..10761
                     /locus_tag="OO448_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6337953.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10777..11199
                     /locus_tag="OO448_002783"
     CDS             10777..11199
                     /locus_tag="OO448_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6337954.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11181..11615
                     /locus_tag="OO448_002784"
     CDS             11181..11615
                     /locus_tag="OO448_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6337955.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11608..14169
                     /locus_tag="OO448_002785"
     CDS             11608..14169
                     /locus_tag="OO448_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6337956.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14166..14495
                     /locus_tag="OO448_002786"
     CDS             14166..14495
                     /locus_tag="OO448_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6337957.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14495..15193
                     /locus_tag="OO448_002787"
     CDS             14495..15193
                     /locus_tag="OO448_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6337958.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15199..15942
                     /locus_tag="OO448_002788"
     CDS             15199..15942
                     /locus_tag="OO448_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6337959.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15879..16511
                     /locus_tag="OO448_002789"
     CDS             15879..16511
                     /locus_tag="OO448_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6337960.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16572..>18430
                     /locus_tag="OO448_002790"
     CDS             16572..>18430
                     /locus_tag="OO448_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6337961.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPA"
BASE COUNT         4207 a         4600 c         5716 g         3907 t
ORIGIN      
        1 cgtcgccgta ttcccgtatt aacagagacc gtagcccgac ggggaactcc ttctgcgcga
       61 gtgtgcggga ataatcaaaa acgatgcaca ccggggttac cgggtacaca tatttcatca
      121 tgccagcgag tccggttctg gcacggaaga aaccggacgt tatgatttag tgcggaaata
      181 tttgtgtagt gttctgtatg ttctcagtaa agagtaatga attatcaaag gtatagtaat
      241 accttttgtt ttcgtggata tttgtaatcc atctgaaaac ccctgctgta gcaagatttt
      301 tcctgtattc gtaaaatgat aactctcctg atttgaatcc ttttaaggtg gcttctataa
      361 ggcatttatt ttttgaaaat cttacattta caaccttacc ctgtcctttt attaaaaccg
      421 tattatcgtt ttcaagaaca agatgaatat tctctgtggc taaatagtaa atgtaatgtg
      481 agacattgtg acgttttagt tcagaataaa accagtgata gtttaaatta tttcgcactt
      541 tatcgaatat ttgtttaaaa atggcaacct gagccattgt agtaccttcc atgtgatatg
      601 agggggcgta gtctgcacga ttatctaaat tgcttcaatc tggtctaacc tgttttctga
      661 gcaattcagt aatgtcactc ttttctttgt ttgcttcagg cgaaactctt ttttctgagc
      721 acagtctccg gcggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgaa
      781 caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg
      841 ttctgcgggt tctgttcttc gttgacatga ggttgccctg tattcagtgt cgctgatttg
      901 tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt
      961 cataattgat tatttgacgt ggtttgatgg cgtagatgca cgttgtgaca tgtagatgat
     1021 aattattatc attttgcggg tcctttccgg cgatccgaca ggttacgggg cggaaggcgc
     1081 gcgggttttc gctatttatg acaattttcc ggtttaaggc gtttccgttc ttcttcgccg
     1141 taacctaatg tttttattta aaacaccccc tgaaaagaaa ggaaacgaca ggtgctgaaa
     1201 acgggctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg
     1261 aagtcaacaa aaagcagctg gctgacattt tcggtgcgag tatccgtacc attcagaact
     1321 ggcaggaaca gggaatgccc gttctgcgag gcggtggcaa gggtaatgag gtgctttatg
     1381 actctgccgc cgttataaga tggtatgccg aaagggatgc tgaaattgag aacgaaaagc
     1441 tgcgccggga agttgaagaa ctgcggcagg ccagcgagac agatctccag ccagggacta
     1501 ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggag ctgaaaaatg
     1561 ccagagactc cgctgaagtg gtggaaaccg cattctgtac tttcgtgctg tcgcggatcg
     1621 caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg cgttttccgg
     1681 aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc atgaacaaag
     1741 cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa cagtcaggtt
     1801 aacaggctgc ggcattttgt ccgcgccggg cttcgctcac tgttcaggcc ggagccacag
     1861 accgccgttg aatgggcgga tgctaattac tatctcccga aagaatccgc ataccaggaa
     1921 gggcgctggg aaacactgcc ctttcagcgg gccatcatga atgcgatggg cagcgactac
     1981 atccgtgagg tgaatgtggt gaagtctgcc cgtgtcggtt attccaaaat gctgctgggt
     2041 gtttatgcct actttataga gcataagcag cgcaacacac ttatctggtt gccgacggat
     2101 ggtgatgccg agaactttat gaaaacccac gttgagccga ccatccgcga tattccgtcg
     2161 ctgctggcgc tggctccgtg gtatggcaaa aagcaccggg ataacacgct cactatgaag
     2221 cgtttttcca atggtcgtgg cttctggtgc ctgggcggta aagcggcaaa aaactaccgt
     2281 gaaaagtcgg tggatgtggc gggttatgat gaacttgctg cctttgatga ggatattgaa
     2341 caggaaggct ctccgacgtt ccttggcgac aaacgtattg aaggctcggt ctggccaaag
     2401 tccatccgtg gctccacgcc caaagtgaga ggcacctgcc agattgagcg tgcagccagt
     2461 gaatccccgc attttatgcg ttttcatgtt gcctgcccgc actgcgggga ggagcagtat
     2521 cttaaatttg gcgacaaaga gacgccgttt ggcctcaaat ggacgccgga tgacccctcc
     2581 agcgtgtttt atctctgcga gcataatgcc tgcgtcatcc gccagcagga gctggacttt
     2641 actgatgccc gttatatctg cgaaaagacc gggatctgga cccgtgatgg cattctctgg
     2701 ttttcgtcat ccggtgaaga gattgagcca cctgacagtg tgacctttca catctggaca
     2761 gcgtacagcc cgttcaccac ctgggtgcag attgtcaaag actggatgaa aacgaaaggg
     2821 gatacgggaa aacgtaaaac cttcgtaaac accacgctcg gtgagacgtg ggaggcgaaa
     2881 attggcgaac gtccggatgc tgaagtgatg gcagagcgga aagagcatta ttcagcgccc
     2941 gttcctgacc gtgtggctta cctgaccgcc ggtatcgact cccagcggga tcgctacgaa
     3001 atgcgcgtat ggggatgggg gccgggtgag gaaagctggc tgattgatcg gcagattatt
     3061 atgggccgcc acgacgatga acagacgctg ctgcgtgtgg atgaggccat caataaaacc
     3121 tatacccgcc ggaatggtgc agaaatgtcg atatcccgta tctgctggga tattggcggg
     3181 attgacccga ccattgtgta tgaacgctcg aaaaagcatg ggctgttccg ggtgatcccc
     3241 attaaagggg catccgtcta cggtaagccg gtggccagca tgcctcgtaa gcgaaacaaa
     3301 aacggggttt accttaccga aatcggtacg gataccgcga aagagcagat ttataaccgc
     3361 ttcacactga cgccggaagg ggatgaaccg cttcccggtg ccgttcactt cccgaataac
     3421 ccggatattt ttgatctgac cgaagcgcag cagctgactg ctgaagagca ggtcgaaaaa
     3481 tgggtggatg gcaggaaaaa aatactgtgg gacagcaaaa agcgacgcaa tgaggcgctc
     3541 gactgcttcg tttatgcgct ggcggcgctg cgcatcagta tttcccgctg gcagctggat
     3601 ctcagtgcac tgctggcgag cctgcaggaa gaggatggtg cagcaaccaa caagaaaaca
     3661 ctggcagatt acgcccgtgc cttatccgga gaggatgaat gacgcgacag gaagaacttg
     3721 ccgctgcccg tgcggcactg catgacctga tgacaggtaa acgggtggca acggtacaga
     3781 aagacggacg gcgagtggag tttacggcca cttccgtgtc tgacctgaaa aaatacattg
     3841 cggagctgga ggtgcagacc ggcatgacac agcgacgcag gggacctgca ggattttatg
     3901 tatgaaaacg cccaccattc ccacccttct gggaccggac ggcatgacat cgctgcgtga
     3961 atatgccggt tatcacggcg gtggcagcgg atttggtggg cagttgcggg cgtggaaccc
     4021 accgggtgaa agtgtggatg cagccctgct gcccaacttt acccgtggca atgcccgcgc
     4081 agacgatctg gtacgcaata acggctatgc tgccaacgcc atccagctgc atcaggatca
     4141 tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg
     4201 ggaggaagac gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga
     4261 ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga
     4321 aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag
     4381 ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc
     4441 gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc
     4501 ggcgctggga tattacgtca gcgaggacgg ctatcctggc tggatgccgc agaaatggac
     4561 atggataccc cgtgagttac ccggcgggcg cgcctcgttc attcacgttt ttgaacccgt
     4621 ggaggacggg cagacccgcg gtgcaaatgt gttttacagc gtaatggagc agatgaagat
     4681 gctcgacacg ctgcagaaca cgcagctgca gagcgccatt gtgaaggcga tgtatgccgc
     4741 caccattgaa agtgagctgg atacgcagtc agcgatggat tttattctgg gcgcgaacag
     4801 taaggagcag cgggaaaggc tgacgggctg gattggtgaa attgccgcgt attacgccgc
     4861 agcaccggtc cgtctgggag gcgcaaaagt gccgcacctg atgccggggg actcactgaa
     4921 cctgcagacg gctcaggaca cggataacgg ctactccgtg tttgagcagt cactgttgcg
     4981 gtatatcgct gccgggctgg gtgtctcgta tgagcaactt tcccggaatt acgcccagat
     5041 gagctactcc acggcacggg ccagtgcgaa cgagtcgtgg gcgtacttta tggggcggcg
     5101 aaaattcgtc gcatcccgtc aggcgagcca gatgtttctg tgctggctgg aagaggccat
     5161 cgttcgccgc gtggtgacgt taccttcaaa agcgcgcttc agctttcagg aagcccgcag
     5221 tgcctggggg aactgcgact ggataggctc cggtcgtatg gccatcgatg gtctgaaaga
     5281 agttcaggaa gcggtgatgc tgatagaagc cggactgagc acctacgaga aagagtgcgc
     5341 gaaacgcggt gacgactatc aggaaatttt tgcccagcag gtccgtgaaa cgatggagcg
     5401 ccgcgcagct ggtcttaaac cgcccgcctg ggcggctgcg gcatttgaat ccgggctgcg
     5461 acaatcaaca gaggaggaga agagtgacag cagagctgcg taatctcccg catattgcca
     5521 gcatggcttt taatgagccg ctgatgcttg aacccgccta tgcgcgggtt ttcttttgtg
     5581 cgcttgcagg ccagcttggg atcagctgcc tgacggatgc agtatccggc gacagcctga
     5641 ctgccggaga ggcacccgcg gcgctggcgt tatccggtga tgatgacgga ccacgacagg
     5701 cccgcagtta tcaggtaatg aacggcatcg ccgtgctgcc ggtgtccggc acgctggtca
     5761 gccggacgcg ggcgctgcag ccgtactcgg ggatgaccgg ttacaacggc attatcgccc
     5821 gtctgcaaca ggctgccagc gacccgatgg tggacggcat tctgctcgat atggacacac
     5881 cgggcggaat ggtggcggga gcatttgact gcgctgacat catcgcccgt gtgcgtgaca
     5941 taaagccggt atgggcgctg gccaatgaca tgaactgcag cgcaggtcag ctgcttgcca
     6001 gtgccgcctc ccggcgtctg gtcacgcaga ccgcccggac aggctccatc ggcgtcatga
     6061 tggctcacag taattacggc gctgcactgg agaaacaggg cgtggaaatc acgctgattt
     6121 acagcggcag ccataaggtg gatggcaacc cctacagcca tctaccggat gatgtccggg
     6181 aaacattgca gtcccggatg gatgcaaccc gccggatgtt tgcacagaag gtgtcggcat
     6241 ataccggcct gtccgtgcag gctgtgctgg ataccgaggc tgcagtgtac agcggtcagg
     6301 aggccattga tgccggactg gctgatgaac ttgttaacag taccgatgcg atcaccgtca
     6361 tgcgtgatgc actggatgca cgtaaatccc gtctctcagg agggcgaatg accaaagaga
     6421 ctcaatcaac aactgtttca gccactgctt cgcaggctga cgttactggc gtggtgcaag
     6481 cgacggaggg cgagaacgcc agcgcggcgc agccggacgt gaacgcgcag atcaccgcag
     6541 cggttgcggc agaaaacagt cgcattatgg ggatcctcaa ctgtgaggag gctcacggac
     6601 gcgaagaaca ggcacgcgtg ctggccgaaa cccccggtat gaccgtggaa acggcccgcc
     6661 gtattctggc cgcagcacca cagagtgcac agacgcgcag tgacactgcg ctggatcgtc
     6721 tgatgcaggg ggcaccggca ccgctggctg caggtaaccc ggcatctgat gccgttaacg
     6781 atttgctgaa cacaccagtg taagggatgt ttatgacgag caaagaaacc tttacccatt
     6841 accagccgct gggcaatagt gacccggctc ataccgcaac cgcgcccggc ggattgagtg
     6901 cgaaagcgcc tgcaatgacc ccgctgatgc tggacacctc cacccgtaag ctggttgcgt
     6961 gggatggcac caccgacggt gctgccgttg gcattcttgc ggttgctgct gaccagacca
     7021 gcaccacact gacgttctac aagtccggca cgttccgtta tgaggatgtg ctctggccgg
     7081 aggctgccag cgacgagacg aaaaaacgga ccgtgtttgc cggaacggca atcagcatcg
     7141 tttaacttta cccttcatca ctaaaggccg cctgtgtggc tttttttacg ggattttttt
     7201 atgtcgatgt acacaaccgc ccagctgctg gcggcaaatg agcagaaatt taagtttgat
     7261 ccgctgtttc tgcgtctctt tttccgtgag agctatccct tcactacgga gaaagtctat
     7321 ctctcacaaa ttccgggact ggtaaacatg gcgctgtacg tttcgccgat tgtttccggt
     7381 gaggttatcc gttcccgtgg cggctccacc tctgaattta cgccgggata tgtcaaaccc
     7441 aagcatgagg tgaatccgca gatgaccctg cgtcgcctgc cggatgaaga tccgcagaat
     7501 ctggcggacc cggcttaccg ccgccgtcgc atcatcatgc agaacatgcg tgacgaagag
     7561 ctggccattg ctcaggtcga agagatgcag gcagtttctg ccgtgctcaa gggcaaatac
     7621 accatgaccg gtgaagcctt cgatccggtt gaggtggata tgggccgcag tgcggcgaac
     7681 aacatcacac agtccggcgt cacggagtgg agcaagcgtg acaagtccac gtatgacccg
     7741 accgacgata tcgaagccta cgcgctgaac gccagcggtg tggtgaatat catcgtgttt
     7801 gacccgaaag gctgggcgct gttccgttcc ttcaaagccg tcaaggagaa gctggatacc
     7861 cgtcgcggct ctcattccga gctggagaca gcggtaaaag acctgggcaa agcggtgtcc
     7921 tataagggaa tgtatggcga tgtggccatc gtcgtgtatt ccggacagta cgtggaaaac
     7981 ggcgtcaaaa agaacttcct gccggacaac acgatggtgc tggggaacac tcaggctcgc
     8041 ggtctgcgca cctatggctg cattcaggat gcggacgcac agcgcgaagg cattaacgct
     8101 tctgcccgtt acccgaaaaa ctgggtgacc accggcgatc cggcgcgtga gttcaccatg
     8161 attcagtcag caccgctgat gctgctggct gatcctgatg agttcgtgtc cgttcaactg
     8221 gcgtaatcat ggcccttcgg ggccattttc tctctgtgga ggagttcatg acgaaagatg
     8281 aactgattgc ccgtcttcag gagctgggtg agcaattgaa ccgcgatgtc agtttgacgg
     8341 gaacgaaaga agaactggcg cttcgtgtgg cagagctgga agaagagctt gatgacacgg
     8401 atgaaactgc cggtcaggac acccctctca gccgggaaaa tgtgctgacc gggcatgaaa
     8461 atgaggtggt atcagcgcag ccggataccg tgattcagga tacggctgaa ctggtcacgg
     8521 tcgtggcact ggtgacgctg catactgatg cacttcacgc cacgcgggat aaacctgtgg
     8581 catttgtgct gccgggaacg gcgtttcgtg tctctgccgg tgtggcagcc gaaatgacag
     8641 agcgcggcct ggccagaatg caataacggg aggcgctgtg gctgatttcg ataacctgtt
     8701 cgatgctgcc attgcccgcg ccgatgaaac gatacgcggg tacatgggaa cgtcagccac
     8761 catgacatcc ggtgagcagt caggtgcggt gatacgtggt gtttttgatg accctgaaaa
     8821 tatcagctat gccggacagg gcgtgcgcgt tgaaggctcc agcccgtccc tgtttgtccg
     8881 gactgatgag gtgcggcagc tgcggcgtgg agacacgctg accatcggtg aggaaaattt
     8941 ctgggtagat cgggtttcgc cggatgatgg cggaagttgt catctctggc ttggacgggg
     9001 cgtaccgcct gccgttaacc gtcgccgctg aaagggggat gtatggccat aaaaggtctt
     9061 gagcaggccg ttgaaaacct cagccgtatc agcaaaacgg cggtgcccgg tgccgccgca
     9121 atggccatta accgcgttgc ttcatccgcg atatcgcagt ctgcgtcaca ggttgcccgt
     9181 gagacaaagg tacgccggaa actggtaaag gaaagggcca ggctgaaaag ggccacggtc
     9241 aaaaatccgc aggccagaat caaagttaac cggggggatt tgcccgtaat caagctgggt
     9301 aatgcgcggg ttgtcctgtc ccgccgcagg cgtcataaaa aggggcagcg ttcatccctg
     9361 aaaggtggcg gcagcgtgct tgtggtgggt aaccgtcgta ttcccggcgc gtttattcag
     9421 caactgaaaa acggccggtg gcatgttatg cagcgtgtgg ccgggaaaaa ccgttacccc
     9481 attgatgtgg tgaaaatccc gatggcggtg ccgctgacca cggcgtttaa acagaatatt
     9541 gaacggatac ggcgtgaacg tcttccgaaa gagctgggct atgcgctgca gcatcaactg
     9601 agaatggtaa taaagcgatg aaacatactg aactccgtgc agccgtactg gatgcactgg
     9661 agaagcatga caccggggcg acgctttttg atggtcgccc cgctgttttt gatgaggaag
     9721 attttccggc aattgccgtt tatctcaccg gcgctgaata cacgggcgaa gagctggaca
     9781 gcgatacctg gcaggcggag ctgcatattg aagttttcct gcctgctcag gtgccggatt
     9841 cagagctgga ttcgtggatg gagtcccgga tttatcctgt gatgagcgat atcccggcac
     9901 tgtcagattt gatcaccagt atggtggcca gcggctatga ctaccggcgc gacgatgatg
     9961 cgggcctgtg gagttcagcc gatctgactt atgtcattat ctatgaaatg tgaggacgct
    10021 atgcctgtac caaatcctac aatgccggtg aaaggtgccg ggaccaccct gtgggtttat
    10081 aaggggagcg gtgaccctta tgcgaacccg ctttcagacg ttgactggtc gcgtctggca
    10141 aaagttaaag acctgacgcc cggcgaactg accgctgagt cctatgacga cagctatctc
    10201 gatgatgaag atgcagactg gactgcgacc gggcaggggc agaaatctgc cggagatacc
    10261 agcttcacgc tggcgtggat gcccggagag caggggcagc aggcgctgct ggcgtggttt
    10321 aatgaaggtg atacccgtgc ctataaaatc cgcttcccga acggcacggt cgatgtgttc
    10381 cgtggctggg tcagcagtat cggtaaggcg gtgacggcga aggaggtgat tacccgcacg
    10441 gtgaaggtca ccaatgtggg acgtccgtcg atggcagaag atcgcagcac ggtgacggcg
    10501 acgaccggca tgacggtgac acccgccagt gcttccgtag tgaaagggca gagcaccacg
    10561 ctgaccgtgg cattccagcc ggatggcgca accgacaaga gcttccgtgc ggtgtctgcg
    10621 gataaaacaa aagccaccgt gtcggtcagt ggtatgacca tcaccgtgaa aggtgttgct
    10681 gcaggcaagg tcaacattcc ggtcgtatct ggtaatggtg agtttgctgc ggttgcagaa
    10741 atcaacgtca ccgccagtta atccggagag tcagcgatgt tcctgaaaac cgaatcattt
    10801 gaacataacg gtgtgaccgt cacgctttct gaactgtcag ccctgcagcg tattgagcat
    10861 ctcgacctga tgaaacggca ggcagaacag gcggagtcag acagcaaccg gaagtttact
    10921 gtggaagacg ccatcagaac cggcgctttt gtggttgcga tgtccctgtg gcataaccat
    10981 ccgaagaaga cgcagatgcc gtccatgaat gaagccgtta aacagattga gcaggaagtg
    11041 cttaccacct ggcccacaga ggcaatttct catgctgaaa acgtggtgta ccggctgtcc
    11101 ggtatgtatg agtttgtggt gaatgatgct cctgaacagg cagaggacgc cgggcctgca
    11161 gagcctgttt ctgcgggaaa gtgttcgacg gtgagctgag ttttgccctg aaactggcgc
    11221 gtgagatggg gcgacccgac tggcgcgcca tgcttgccgg gatgtcatcc acggagtatg
    11281 ccgactggca ccgcttttac agtacccatt attttcatga tgttctgctg gatatgcact
    11341 tttccgggct gacgtacacc gtgctcagcc tgtttttcag cgatccggag atgcatccgc
    11401 tggatttcag tctgctgaac cggcgcgagg ctgacgaaga gcctgaagat gatgtgctga
    11461 tgcagaaagc ggcagggctt gccggaggtg tccgctttgg cccggacggg aatgaagtta
    11521 tccccgcttc cccggatgtg gcggacatga cggaggatga cgtaatgctg atgacagtat
    11581 cagaagggat cgcaggagga gtccggtatg gctgaaccgg taggcgatct ggtcgttgat
    11641 ttgagtctgg atgcggccag atttgacgag cagatggcca gagtcaggcg tcatttttcc
    11701 ggtacggaaa gtgatgcgaa aaaaacagcg gcagtcgttg aacagtcgct gagccgacag
    11761 gcgctggctg cacagaaagc ggggatttcc gtcgggcagt ataaagccgc catgcgtatg
    11821 ctgcctgcac agttcaccga cgtggccacg cagcttgcag gggggcagaa tccctggctg
    11881 atcctgctgc aacagggtgg tcaggttaag gactccttcg gcgggatgat ccccatgttc
    11941 agggggcttg ccggcgcgat caccctgccg atggtggggg ccacctcgct ggcggtggcg
    12001 accggtgcgc tggcgtatgc ctggtatcag ggcaactcaa ccctgtccga tttcaacaaa
    12061 acgctggtcc tttccggtaa tcaggcggga ctgacggcag atcgtatgct ggtcctgtcc
    12121 agagccgggc aggcggcagg gctgacgttt aaccagacca gcgagtcact cagcgcactg
    12181 gttaaggcgg gggtaagcgg tgaggctcag attgcgtcca tcagccagag tgtggcgcgt
    12241 ttctcctatg catccggcgt ggaggtggac aaggtcgctg aagccttcgg gaagctgacc
    12301 acagacccga cgtcggggct gacggcgatg gcacgccagt tccataacgt gacggcggag
    12361 cagattgcgt atgttgctca gttgcagcgt tccggcgatg aagccggggc attgcaggcg
    12421 gcgaacgagg ccgcaacgaa agggtttgat gaccagaccc gccgcctgaa agagaacatg
    12481 ggcacgctgg agacctgggc agacaggact gcacgtgcat tcaaatccat gtgggatgcg
    12541 gtgctggata ttggtcgtcc tgataccgcg caggagatgc tgattaaggc agaggctgcg
    12601 tttaagaaag cagacgacat ctggaatctg cgcaaggatg attatttcgt taacgatgaa
    12661 gcacgggcgc gttactggga tgatcgtgaa aaaaaacgcc tggagcggga tgcagcccaa
    12721 aagagggtag atcaacagcg tcaacaggac aaaaatgcgc agcagcagag cgataccgaa
    12781 gcgtcacggc tgaaatatac cgaagaggcg cagaaggctt acgaacggct gcagacgccg
    12841 ctggagaaat ataccgcccg tcaggaagaa ctgaacaagg cactgaaaga cggaaaaatc
    12901 ctgcaggcag attacaacac gctgatggcg gcggcgaaaa aggattatga agcgacgctg
    12961 aaaaagccga aacagtccgg cgtgaaggtg tatgcgggcg atcgtcagga agacagtgct
    13021 catgcggccc tgctgacgct tcaggcagaa ctccggacgc tggagaagca tgccggagcg
    13081 aatgagaaaa tcagccagca gcgccgggat ttgtggaagg cagaaagtca gttcgcggta
    13141 ctggaggagg cggcacaacg tcgccagctg tccgcacagg agaaatccct gctggcgcat
    13201 aaagacgaga cgctggagta caaacgccag ctggctgcac ttggtgacaa ggttacgtat
    13261 caggagcacc tgaatgcgct ggcgcagcag gcggataaat tcgcacagca gcaacgggca
    13321 aaacgggcag ccattgatgc gaaaaaccgg gggctgactg accggcaggc agcgcgggaa
    13381 gccacggaac agcgcctgaa ggaacagtat ggcgataatc ctctggcgct gaataacgtc
    13441 atgtcagagc agaaaaagac ctgggcggct gaagaccagc ttcgcgggag ctggatggca
    13501 ggcctgaagt ccggctggag tgagtgggaa gagagcgcca cggacagtat gtcacaggtt
    13561 aaaagtgcag ccacgcagac ctttgatggt attgcacaga atatggcggc gatgctgacc
    13621 ggcagtgaac agaactggcg cagcttcacc cgctccgtgc tgtccatgat gacagaaatt
    13681 ctgcttaagc aggcaatggt ggggattgtc gggagtatcg gcagcgccat tggcggggct
    13741 gttggtggcg gcgcatccgc gtcaggtggt acagccattc aggccgctgc ggtgaaattc
    13801 cattttgcaa ccgggggatt tacgggaacc ggcggcaaat atgagccagc ggggattgtt
    13861 caccgtggtg aatttgtctt cacgaaggag gcaaccagcc ggattggcgt ggggaatctc
    13921 taccggctga tgcgcggcta tgcggaaggt ggttatgtgg gcggtgccgg aagtccggcg
    13981 cagatgcggc gggctgaagg cattaatttt aatcagaaca atcacgtggt gattcagaac
    14041 gacggtacga atggtctgcc aggtccacag atgatgaagg ccgtgtatga catggcccgc
    14101 aagggtgccc gtgatgaaat tcagacacag atgcgtgatg gtggcctgtt ctccggaggt
    14161 ggacgatgaa aaccttccgc tggaaagtga aacccggtat ggatgtggct tcggcccctt
    14221 ctgtaagaaa ggtgcgcttt ggtgatggct attcccagcg agcgcctgcc gggctgaatg
    14281 ccaacctgaa aacgtacagc gtgacgcttt ctgtcccccg tgaggaggcc atggtactgg
    14341 agtcgtttct ggaagagcac gggggctgga aagcctttct gtggacgccg ccttatgagt
    14401 ggcggcagat aaaggtgacc tgcgcaaaat ggtcgtcgcg ggtcagtatg ctgcgtgttg
    14461 agttcagcgc agagtttgaa caggtggtga actgatgcag gatatccggc aggaaacact
    14521 gaatgaatgc acccgtgcgg agcagtcggc cagcgtggtg ctctgggaaa tcgatctgac
    14581 agaggtcggt ggagaacgtt attttttctg taatgagcag aacgaaaaag gtgagccggt
    14641 cacctggcag gggcgacagt atcagccgta tcccattcag gggagtggtt ttgaactgaa
    14701 tggcaaaggc accagtacgc gccccacgct gatggtttct aacctgtacg gtatggtcac
    14761 cgggatggcg gaagatctac agagtctggt cggcggaacg gtggtccggc gtaaggttta
    14821 cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agtgacgccg atccggagca
    14881 ggaggtgatc agccgctggc gcattgagca gtgcagcgaa ctgagcgcgg tgagtgcctc
    14941 ttttgtactg tccacgccga cggaaacaga tggcgctgtt tttccgggac gtatcatgct
    15001 ggccaacacc tgcacctgga cctatcgcgg cgatgagtgc ggttatcacg gtccggcggt
    15061 cgcggatgaa tatgaccagc caacgtccga catcacgaag gataaatgca gcaaatgcct
    15121 gagtggctgt aagtttcgca ataacgtcgg caactttggc ggcttccttt ccattaacaa
    15181 actttcgcag taaatcccat gacagagaca gaatcagcga ttctggcgca cgcccggcga
    15241 tgtgctccag cggagtcgtg cggcttcgtg gtaagaacgc cggaggggga aagatatttc
    15301 ccctgcgtga atatctccgg tgagccggag gcgtatttcc gtatgtcgcc ggaagactgg
    15361 ctgcaggcag agatgcaggg tgagattgtg gcactggtcc acagccaccc cggtggtctg
    15421 ccctggctga gtgaggccga ccggcggctg caggtgcaga gtgatttgcc gtggtggctg
    15481 gtctgccggg gggcgattca caagttccgc tgtgtgccac atcttaccgg gcggcgcttt
    15541 gagcacgggg tgacggactg ttacacgctg ttccgggatg cttaccatct ggcggggatt
    15601 gagatgccgg attttcatcg tgaggatgac tggtggcgtc acggtcagaa tctctatctg
    15661 gataatctgg aggccacagg gctgtatcag gtgccgttgt cagcggcgca gccgggcgat
    15721 gtgctgctgt gctgttttgg ttcatcggtg ccgaatcatg ccgccattta ctgtggtgac
    15781 ggcgagctgc tgcaccatat tcctgaacaa ctgagcaaac gagagaggta taccgacaaa
    15841 tggcagcgac gcacacactc cctctggcgt caccgggcat ggcacgcatc tgcctttacg
    15901 gggatttgca acgatttggc cgccgcatcg accttcgtgt gaaaacgggt gccgaagcca
    15961 tccgggcgat ggccacacag ctccctgcgt ttcgtcagaa gctgagcgac ggctggtatc
    16021 aggtgcgtat tgccgggcag gatgttagca cgtccggatt aacggcacag ttacatgagg
    16081 ctctgcctga cggcgctgtg attcatattg ttcccagagt cgccggggcc aagtcaggtg
    16141 gtgtattcca gattgtcctg ggagcagccg ccattgccgg atcattcttt accgccggag
    16201 ccacccttgc agcatggggg gctgccattg gtgccggtgg tatgaccggc attctgtttt
    16261 ctctcggtgc cagtatggtg ctcggcggtg tggctcagat gctggcaccg aaagccagaa
    16321 ctccccgcac acagacaacg gataacggta agcagaacac ctatttctcc tcactggata
    16381 acatggttgc ccagggcaat gttatgcctg ttctgtatgg tgaaatgcgc gtggggtcac
    16441 gtgtggtatc tcaggagatc agcacggcag acgaagggga cggtggtcag gttgtggtga
    16501 ttggtcgctg atgcaaaatg ttttatgtga aatcgcctcc gggcggtttt atcgtttatg
    16561 gagcatgacg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa gcgaaggaca
    16621 acctgaagtc cacgcagctg ctgagtgtga tcgatgccat cagcgaaggg ccggttgaag
    16681 gtccggtgga tggattaaaa agcgtgctgc tgaacagtac gccggtgctg gacagtgagg
    16741 ggaataccaa catctccggc gtcacggtgg tgttccgggc aggtgagcag gagcagtcac
    16801 cgccggaggg atttgaatcc tccggctccg agacggtgct gggtacggaa gtgaaatatg
    16861 acacgccgat cacccggacc atcacgtcgg caaacattga ccgactgcgt tttaccttcg
    16921 gtgtacaggc actggtggaa accacctcaa agggagacag gaatccgtct gaagttcgcc
    16981 tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc accattaagg
    17041 gcaaaaccac ctcgcagtat ctggcctcgg tggtggtgga taacctgccg ccgcgcccgt
    17101 ttaatatccg gatgcgcaga atgacgccgg acagcaccac agaccagctg cagaacaaaa
    17161 cgctctggtc gtcatacacc gaaatcatcg atgtgaaaca gtgctacccg aacacggcac
    17221 tggtcggcgt acaggtggat tcggagcagt tcggcagcca gcaggtgagc cgtaattatc
    17281 atcttcgcgg gcgcattctg caggtgccgt cgaactataa tccgcagacg cggcaataca
    17341 gcggtatctg ggacggaacg ttaaaaccgg catacagcaa caacatggcc tggtgtctgt
    17401 gggatatgct gacccatccg cgctatggca tggggaaacg tcttggtgcg gcagatgtgg
    17461 ataaatgggc gctgtatgtc atcggccagt actgtgacca gtcggtgccg gacggctttg
    17521 gcagcacgga gccgcgcatc acctgtaatg cctggctgac cacacagcgc aaggcgtggg
    17581 atgtgctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc
    17641 tgacgttcgt gcaggaccga cagtcggata aggtgtgggc ctataaccgc agtaatgtgg
    17701 tgatgccgga tgatggcgcg ccgttccgct acagtttcag cgccctgaaa gaccgccata
    17761 atgccgttga ggtgaactgg attgacccgg ataacggctg ggagacggcg acagagcttg
    17821 tggaggatac gcaggccatt gtccgttacg gtcgtaacgt cacgaagatg gatgcctttg
    17881 gctgtaccag ccgggggcag gcacaccgcg ccgggctgtg gctgattaaa acggaactgc
    17941 tggagacgca gaccgtggat ttcagcgtgg gtgctgaagg gcttcgtcat gtaccgggcg
    18001 atgtcattga aatctgcgat gatgactatg ccggtatccg caccggcggg cgcgtgctgg
    18061 cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg ccatcctccg
    18121 gcaccacgct gataagcctg gttgacgggc aggggaatcc ggtcagcgtg gaggtccagt
    18181 ccgtcaccga cggcgtgaag gtaaaagtga gccgtgttcc tgacggtgtt gctgaataca
    18241 gcgtgtgggg gctgaagctg ccgacgctgc accagcgatt gttccgctgc gtgagtatcc
    18301 gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg gaaaaagaag
    18361 ccatcgtgga taacggggcg cactttgacg gcgaccagag cggcacggtg aatggtgtca
    18421 cgccgccagc
//
LOCUS       DAJTVJ010000083        18373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000083 DAJTVJ010000000
VERSION     DAJTVJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.083"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            690..1460
                     /locus_tag="OO448_002791"
     CDS             690..1460
                     /locus_tag="OO448_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6337962.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1451..1924
                     /locus_tag="OO448_002792"
     CDS             1451..1924
                     /locus_tag="OO448_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6337963.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2032..2571
                     /gene="dnaT"
                     /locus_tag="OO448_002793"
     CDS             2032..2571
                     /gene="dnaT"
                     /locus_tag="OO448_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6337964.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2574..3311
                     /gene="dnaC"
                     /locus_tag="OO448_002794"
     CDS             2574..3311
                     /gene="dnaC"
                     /locus_tag="OO448_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6337965.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3360..3854
                     /locus_tag="OO448_002795"
     CDS             3360..3854
                     /locus_tag="OO448_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6337966.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4108..6399
                     /gene="opgB"
                     /locus_tag="OO448_002796"
     CDS             4108..6399
                     /gene="opgB"
                     /locus_tag="OO448_002796"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6337967.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OO448_002797"
     CDS             complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="OO448_002797"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6337968.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7699..8613
                     /locus_tag="OO448_002798"
     CDS             7699..8613
                     /locus_tag="OO448_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6337969.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8828..10179
                     /locus_tag="OO448_002799"
                     /pseudo
     CDS             8828..10179
                     /locus_tag="OO448_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="OO448_002800"
                     /pseudo
     CDS             complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="OO448_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="OO448_002801"
     CDS             complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="OO448_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6337970.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12720..14009
                     /locus_tag="OO448_002802"
     CDS             12720..14009
                     /locus_tag="OO448_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6337971.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14006..15472
                     /gene="hpaE"
                     /locus_tag="OO448_002803"
     CDS             14006..15472
                     /gene="hpaE"
                     /locus_tag="OO448_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6337972.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15474..16325
                     /gene="hpaD"
                     /locus_tag="OO448_002804"
     CDS             15474..16325
                     /gene="hpaD"
                     /locus_tag="OO448_002804"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6337973.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16335..16715
                     /locus_tag="OO448_002805"
     CDS             16335..16715
                     /locus_tag="OO448_002805"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6337974.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16826..17629
                     /gene="hpaH"
                     /locus_tag="OO448_002806"
     CDS             16826..17629
                     /gene="hpaH"
                     /locus_tag="OO448_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6337975.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17640..>18373
                     /gene="hpaI"
                     /locus_tag="OO448_002807"
     CDS             17640..>18373
                     /gene="hpaI"
                     /locus_tag="OO448_002807"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6337976.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEA"
BASE COUNT         4477 a         4915 c         4672 g         4309 t
ORIGIN      
        1 tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat
       61 cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt attcaggata
      121 ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat
      181 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
      241 agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt
      301 gttaggaata cttaatgaaa aacagaaatc acattcattt aatttataat taaatgatat
      361 cccttcccgc agaatgaaaa atcacacaac caatatttat tcaatgaaat ctgatggttt
      421 taacttgtta acattaaaaa acgtgcccgg ttatctatta tcctgattcg taaaggccaa
      481 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
      541 tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt
      601 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      661 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      721 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      781 atgagctttc ctcacgactg ggtcgggcgt tggggatgga cagcgtcgaa agttctatct
      841 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      901 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      961 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
     1021 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
     1081 gcaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
     1141 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccgcagatc aacttttgcc
     1201 tcaccgcttt cgccgccacc accatttccg gattgctttt gcaacttccc actttcagca
     1261 atacacccac tatcgcgatg gccgccagcg ttctgctgct ggtgccggga tttccgttga
     1321 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
     1381 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
     1441 ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata tgatcctcgc
     1501 cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cattacgctg
     1561 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1621 gaatattgag tggtcaacct ttatggcctc catgctggtc ggcaccattg gtattcaatg
     1681 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1741 cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agttaggtta
     1801 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1861 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1921 ataaaattgc ctcgcttaaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1981 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     2041 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     2101 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     2161 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     2221 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     2281 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     2341 ctggcggcgc tatggggtgt ggcactaaga gagccggtga ccaccgaaga actggcctca
     2401 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2461 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2521 aatacggtca gcgaacctga cagtcaaatt ccaccaggat tcagagggta acgatgaaaa
     2581 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2641 tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc cgctccgccg
     2701 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2761 ggccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2821 atgcgttaag caaagcgcgc cagtacgtcg aagagtttga cggcaacatc gccagcttca
     2881 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2941 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     3001 aagatacctt caggaatagc ggtaccagtg aagaacaact gcttaacgat ctgagcaacg
     3061 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     3121 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     3181 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     3241 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta
     3301 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     3361 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3421 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3481 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3541 gcagcgggaa ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat
     3601 actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3661 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3721 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3781 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3841 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3901 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3961 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     4021 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     4081 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     4141 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     4201 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     4261 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     4321 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     4381 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     4441 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4501 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4561 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4621 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4681 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4741 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4801 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4861 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4921 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4981 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     5041 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     5101 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     5161 gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc
     5221 gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     5281 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     5341 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     5401 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     5461 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5521 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5581 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5641 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5701 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5761 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg
     5821 tgtgtctcgc aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca
     5881 tggcagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat
     5941 gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc
     6001 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagtg ggatttcccg tccagagtcg
     6061 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     6121 ctgccaaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     6181 cctattccgg tacgcgtagg caatgaagaa caaacccttg tgctgggcaa tgaagtgacc
     6241 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     6301 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     6361 ggcatggtgg aaattaaagt ggtggaacgt gaaggataat tcctgactga tgtgtaggtc
     6421 gcataaggtg cgacctacac accttctgcg gcctacaaaa catcattgag aaacagaaaa
     6481 aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca
     6541 gaaagtaatt acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc
     6601 tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcagcagtga tttttccttc
     6661 cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tacccatcat
     6721 cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc
     6781 gacaaatacc accgtgccgc cgtgacgaat caaattcacg gtgttattca tcgcatgttg
     6841 attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg
     6901 cgcgtcaaaa tcttcggctg acgggtccag taaaggtaat tccagacgcg ttgccacatg
     6961 ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat
     7021 tgccgccgcg cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc
     7081 aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgctgccgc
     7141 ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact
     7201 aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc
     7261 gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc
     7321 atttttaaga tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc
     7381 taaaacacgt ggataactaa aaaatggttg atttccaccc caggcatgaa tatcggtacc
     7441 gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc
     7501 gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat
     7561 gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga
     7621 acggaaaatc tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg
     7681 actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag
     7741 gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg
     7801 gcggaaatgt acaacatcag ccgcaccaca gtgcgtcata ttctcagcca cttacgcgaa
     7861 tgcggcgtct tgacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccatgat
     7921 gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct
     7981 tttttcacga tgataaacca gcgccagtta cgcccagggg aaacgttttc tgaactgcaa
     8041 ctggctcggg ccgcgggtgt cagcccggtt gtagtaagag aatatctttt aaaattcggc
     8101 cgttataatc ttattcacag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa
     8161 tcctacgccg agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac
     8221 tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt
     8281 catcggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga
     8341 gattttcact ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag
     8401 atcatctcag tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc
     8461 aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg ccgcagtgac
     8521 cttgatgcca cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc aatgattcga
     8581 tccattaatg agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta
     8641 gcgggtgcac ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac
     8701 aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc
     8761 gttaatgtca accttgcgta attcacaatt acgtaaacaa ttaaaattat aaatacagga
     8821 gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc
     8881 gtcggcagat attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg
     8941 cattcaaacc accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg
     9001 cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga
     9061 aatcggcgct ttgctctccg tgttttcact cgcttacggg attgcgcaac ttccttgcgg
     9121 cccactattg gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc
     9181 actgttccag gcaatgtctg gcatggtgca caactttacg cagttcgtgt tggtgcgtat
     9241 cggtatgggg attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg
     9301 gttcaacatc aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg
     9361 cgttgccgta agcccaccga ttctggcggc gatgatgctg gtgatgggct ggcgcgggat
     9421 gtttattacc attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg
     9481 caaccgcgag cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt
     9541 caatgcccgc cgagatccgc tcagttttgc cgaatggcgc agcctgttcc gtaaccgtac
     9601 aatgtgggga atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc
     9661 ctggcttcct ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat
     9721 ggcggctatc cctttcctgt ttggggctgc cgggatgctg gtcaacggtt acgttactga
     9781 ctggctggtc aaagggggaa tggctccgat taaaagccgt aagatctgca ttattgccgg
     9841 gatgttctgt tctgccgcct ttacgctggt cgtaccgcaa gcgacaacat ccatgacagc
     9901 ggttctgctg attggcatgg cactgttctg tattcaacat cctgctgggg cttgatccac
     9961 gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt tgccagcttc
    10021 atctgcgcct cttttgcgcc gatcattact ggttttattg ttgataccac ccactcattc
    10081 cgtctggcac taatcatctg cggttgcgtc accgcagcgg gggcactggc gtacatcttc
    10141 ctggttcgtc agccgatcaa cgacccacgt aaagattaat ctccttatgc ccgataacat
    10201 tttgcttatc gggcattttc atggcgatta aaatgtttcc cagttctcct cgctatctgc
    10261 cacggccatt ttacgcggcg cagctggcgt cacggttttt accacagccg atgtttcacg
    10321 ctgctgttgc tgaatccgga acactgccac tgcttcggtc aggcgactgg cctgctcttc
    10381 cagcgcggcg gcggcagcgg cagactcttc caccagcgcg gcgttctgtt gagttacccg
    10441 gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc
    10501 agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg gcgctgacaa tctccgccat
    10561 tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc
    10621 aatcaggctt ttaatttcac gagccgcctg ggcgctgcgc tgggccagat tacgcacttc
    10681 tcccgcgacc accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct caaccgccgc
    10741 gttcaaagcc agaatattgg tctggaaggc aatgccgtca attacgctga taatatcggc
    10801 gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat ctaccacttt
    10861 gccgccgcgt tgcgccgttt cagaagcact taacgccaga tggctggcct ggcgcgcatt
    10921 ttcggcgttc tgcttcaccg ttgcggtcag ttactccatg ctggctgccg tctcttccag
    10981 cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct
    11041 ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc
    11101 tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc catccacctc
    11161 aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat
    11221 tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac
    11281 gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt tatcgctgac
    11341 ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc
    11401 gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaacag
    11461 ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc
    11521 tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc agtttttttc
    11581 cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc
    11641 aatattgttc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg
    11701 caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac
    11761 agtgaaattt tctttgtcat tctttaaggc attaaagaac agaccgcctg atgtcagttg
    11821 taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga tacgttttaa
    11881 catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc aaaaacttta
    11941 tgaaaaggtg taacccacca tccatggaaa aaccaggcgc tggttatcag atactaaaaa
    12001 gttattcatt atcgcccggg atggcttctt gtcgggaatt gcccagagca ataaattcct
    12061 ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca atctgccgat
    12121 aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct ttggttagcg
    12181 agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt
    12241 ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat
    12301 cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac tgttgctcag
    12361 tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct cgcgcctgga
    12421 gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca aaatcattaa
    12481 tatagaaaca gttaatcgtc acgttaccgg gactgcgctg cccctgcaat tcctgatttt
    12541 atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta tctttaaatg
    12601 taaatagttt gttaattaga tcacatttac atcacttact tttgccaaac tacgcaatcg
    12661 ggaataaaag caatcattaa catattaatg attaagatga tcgaatccga ggagtggtaa
    12721 tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat gcatggcagg
    12781 aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg tttattaaac
    12841 cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt gaaaaggtac
    12901 tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc gacgaaagta cgtgaagaag
    12961 atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg ccggaagaga
    13021 gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc attggcgaaa
    13081 ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac gggcgtcctg
    13141 ccgatcactg gaataccgcc gatttacaac gtaacgccgc gcagttgctg agcgccctga
    13201 gcgaatttgc cacgctgaat ccaggcgacg ccattctgct cggcacgcca caggcgcgcg
    13261 tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga aggtttcccg ccgctggaaa
    13321 atccggtagt ggacgaacgt gaagtgacca cgcacaagag cttcccaacg cagccacacc
    13381 cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc gaactggaat
    13441 ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc aaataccctc actggcgata
    13501 accagacctc cgtgcgtcca aacaatattg aatacatgca ctatgaagcg gagctggtgg
    13561 tagttattgg caaacaggcg cgtaacgtca gcgaagccga tgccatggat tatgtcgcgg
    13621 gctacaccgt gtgtaacgac tacgccattc gtgactatct ggaaaactac taccgcccta
    13681 acctgcgggt aaaaagccgc gacggactga cgccgatgct ttcaaccatc gtgccgaaag
    13741 aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc gagttacgcc
    13801 agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc tacttaagtg
    13861 aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacacccaaa ggcttatctg
    13921 acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc ctggtgaacc
    13981 gaattgtgag tgaggataca gcgaaatgaa aaaagtaaat cattggatca acggcaaaaa
    14041 tgttgcaggt aacgactact tccagaccac caatccggca acgggtgaag tgctggcgga
    14101 tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga aagaggcgtt
    14161 cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc gtctgggcga
    14221 tctgatcgac cagaacgtgc cagagatcgc cgcgatggaa accgcggaca ccggcctgcc
    14281 gatccatcag accaaaaatg tgttgatccc acgcgcttcc cacaactttg aatttttcgc
    14341 ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga tgctcaacta
    14401 cacgctggtg cagccggtgg gcgtttgtgc gctggtatcg ccgtggaacg taccgtttat
    14461 gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg tactgaaaat
    14521 gtcggaactc tccccgctga ccgctgacag cctgggtgag ctggcgctgg aagccgatat
    14581 tccggcaggc gtgctgaacg tggtacaggg ctacggcgca accgcagggg atgcgctggt
    14641 tcgtcatcat gacgtacgtg ccgtgtcgtt caccggcggt acggccaccg ggcgcaacat
    14701 catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat cgccggtgct
    14761 gatttttgaa gatgccgata ttgaacgcgc gctggacgcc gccctgttca ccatcttctc
    14821 gatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaaa gcatctaccc
    14881 ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga
    14941 tccgaatacc caggttggcg cgcttattag ccagcagcac tgggaaaaag tctccggcta
    15001 tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg ataaaccgtc
    15061 cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaaccgtgc tggcagacgt
    15121 tgataaccgt atgcgagtcg cccaggaaga gattttcggg ccggtcgcct gcctgctgcc
    15181 gtttaaagac gaagccgaag gcttacgtct ggcaaacgac gtggagtacg gcctcgcgtc
    15241 gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca ttgaagctgg
    15301 catggtgttc gtcaacaccc agaacgtgcg tgacctgcgc cagccatttg gcggcgtaaa
    15361 agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa
    15421 gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct gatatgggta
    15481 agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatcactct gaactgccag
    15541 ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc agcaagcgtt
    15601 gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg gtcaacagtg
    15661 cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac gagctgccgc
    15721 attttatccg cgatatgacc tacaactacg agggcaaccc ggagttgggg cagcttattg
    15781 ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca caacattccc agcctgaaac
    15841 tggaatacgg cacgctggtg ccgatgcgct acatgaatga agacaagcac ttcaaagtgg
    15901 tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag ctgggcgaag
    15961 cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc agcggttcgt
    16021 tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc tacacccgcg
    16081 agttcgaccg ccagatggac gagcgcgtgg tgaagttgtg gcgcgaaggc cagttcaaag
    16141 agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc aatatgcacg
    16201 acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag gtggagttta
    16261 tcaccgagct attcccaagc tctggcaccg gtcaggttaa cgctgttttc ccgcttcccg
    16321 cgtaaggacg ttttatgccg cactttatcg ttaaatgcag tgacaacatc cgcgaagaag
    16381 ccgacctgcc ggggttgttc gccaaagtga atccgacgct ggcagccacg ggtatttttc
    16441 cgctggcggg tattcgcagc cgcgtgcatt gggtcgatac ctggcagatg gccgacgggc
    16501 agcgtgatta tgccttcgtg catatgacgt tgaaaatcgg cgcaggtcgc agcctggaaa
    16561 gccgccagca ggcgggtgaa atgctgtttg aactgattaa aacgcacttc gccgccctga
    16621 tggagagccg cctgctggcg ttgtcgtttg agattgaaga gctgcatccg acgctgaatt
    16681 ttaaacaaaa caacgtgcac gcattgttta agtagcgcgc agattgcccg gtggcgctgc
    16741 gcttaccggg cctacaaaac cccaaaccgt acaccgtagg ccggataagg cgcagccgca
    16801 tccggcaatg ccacaggata tcgctatgtt cgataaacac acccacaccc tgatcgccca
    16861 gcgtctggat caggcagaaa aacagcgcga acagatccgc gcgatctcgc tggattaccc
    16921 ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa tgggttcgcc tgaaaatcgc
    16981 cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc tcgaaagcga tgcaggcaag
    17041 ctcgcagatc agcgaaccgg attacggcgc gctgctggat gacatgttct tccacgatgg
    17101 cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc tggctttcgt
    17161 gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca acgccacgga
    17221 ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc cacaacatcg atccggaaac
    17281 ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac gccgccaacg ccggggtgat
    17341 cctcggcggt cgtccgatca aaccagacga actggatctg cgttggatct ccgccctgat
    17401 gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct ggcgtgctga atcatccggc
    17461 aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat gacgtacaac tggaagccgg
    17521 gcaaatcatt ctcggtggtt cgttcacccg cccggttccg gcgcgtaagg gcgacacctt
    17581 ccacgtcgat tacggcaaca tgggttccat tagctgccgc tttgtttaag gagagaacga
    17641 tggaaaacag ctttaaagcg gcgctgaaag caggccgtcc gcagatcgga ttatggctgg
    17701 ggctgagtag cagctacagc gcagagttac tggccggagc aggattcgac tggttgttga
    17761 tcgacggtga gcacacgccg aataacgtgc aaaccgtgct cacccagcta caggcgattg
    17821 cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg caaatcaaac
    17881 aactgctgga cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac gctgacgaag
    17941 cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg tattcgcggt gtgggcagtg
    18001 cgctggcccg cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa gccaacgatc
    18061 aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat gaagaactta ccgcagattc
    18121 tggacgtgga aggcgtcgac ggcgtgttta tcggcccggc ggatctgagc gccgatatgg
    18181 gttatgccgg taatccgcag cacccggaag tacaggccgc cattgagcag gcgatcgtgc
    18241 agatccgcga agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa ctggcaaaac
    18301 gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc ctgctcgccc
    18361 gcgccgccga agc
//
LOCUS       DAJTVJ010000084        18216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000084 DAJTVJ010000000
VERSION     DAJTVJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.084"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            16..1092
                     /locus_tag="OO448_002808"
     CDS             16..1092
                     /locus_tag="OO448_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6337977.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1485..1826)
                     /locus_tag="OO448_002809"
     CDS             complement(1485..1826)
                     /locus_tag="OO448_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6337978.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1839..2711)
                     /gene="copD"
                     /locus_tag="OO448_002810"
     CDS             complement(1839..2711)
                     /gene="copD"
                     /locus_tag="OO448_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCR6337979.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2715..3089)
                     /gene="yobA"
                     /locus_tag="OO448_002811"
     CDS             complement(2715..3089)
                     /gene="yobA"
                     /locus_tag="OO448_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6337980.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3228..3458
                     /gene="holE"
                     /locus_tag="OO448_002812"
     CDS             3228..3458
                     /gene="holE"
                     /locus_tag="OO448_002812"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6337981.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3560..4216
                     /locus_tag="OO448_002813"
     CDS             3560..4216
                     /locus_tag="OO448_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6337982.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4240..4902
                     /gene="exoX"
                     /locus_tag="OO448_002814"
     CDS             4240..4902
                     /gene="exoX"
                     /locus_tag="OO448_002814"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6337983.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4899..6959)
                     /gene="ptrB"
                     /locus_tag="OO448_002815"
     CDS             complement(4899..6959)
                     /gene="ptrB"
                     /locus_tag="OO448_002815"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6337984.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7168..7827)
                     /locus_tag="OO448_002816"
     CDS             complement(7168..7827)
                     /locus_tag="OO448_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6337985.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8154..8510)
                     /locus_tag="OO448_002817"
     CDS             complement(8154..8510)
                     /locus_tag="OO448_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6337986.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8577..8867)
                     /gene="yebG"
                     /locus_tag="OO448_002818"
     CDS             complement(8577..8867)
                     /gene="yebG"
                     /locus_tag="OO448_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCR6337987.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9001..10179
                     /gene="purT"
                     /locus_tag="OO448_002819"
     CDS             9001..10179
                     /gene="purT"
                     /locus_tag="OO448_002819"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6337988.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10235..10876)
                     /gene="kdgA"
                     /locus_tag="OO448_002820"
                     /gene_synonym="eda"
     CDS             complement(10235..10876)
                     /gene="kdgA"
                     /locus_tag="OO448_002820"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6337989.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10913..12724)
                     /gene="edd"
                     /locus_tag="OO448_002821"
     CDS             complement(10913..12724)
                     /gene="edd"
                     /locus_tag="OO448_002821"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6337990.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12959..14434)
                     /gene="zwf"
                     /locus_tag="OO448_002822"
     CDS             complement(12959..14434)
                     /gene="zwf"
                     /locus_tag="OO448_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6337991.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14772..15641
                     /gene="hexR"
                     /locus_tag="OO448_002823"
                     /gene_synonym="yebK"
     CDS             14772..15641
                     /gene="hexR"
                     /locus_tag="OO448_002823"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6337992.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRTVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15769..17211
                     /gene="pyk"
                     /locus_tag="OO448_002824"
     CDS             15769..17211
                     /gene="pyk"
                     /locus_tag="OO448_002824"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6337993.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17271..18113)
                     /locus_tag="OO448_002825"
     CDS             complement(17271..18113)
                     /locus_tag="OO448_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR6337994.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4672 a         4575 c         4392 g         4577 t
ORIGIN      
        1 tggagaagtt aaaacatgca gcggaaaaaa tacgatccca atttgcctag aaacttaaca
       61 tatcgaagga gagacaaagc atattactgg cgcaaccctc tgacgaaaga agaatttaca
      121 ctaggtaaaa tttcaagaag agatgcagta acgcaggcaa ttgaagcaaa tcattatata
      181 tacaaaaact actctcccgc tgccttaatt gaaaaactta aagggttcga ctcatttact
      241 atggcagact ggattgaacg ttacaaaacg attcttataa ggagaaaagt gtccagaaat
      301 acttataaaa ttcgggcaaa tcaactggag acaataaaag aaaaattggg agagatttta
      361 ctgacagaaa taaccactcg ccatattgcc gagtttcttg atttgtggat tgaaggaggg
      421 aaaaacacaa tggcaggatc aatgcgttct gtgttgtctg atatgtttcg cgaggccatt
      481 gttgaaggac gtatatctca aaatccagtc acgccaacaa gagcaccgaa aatagtagtt
      541 acaagagaac gactgaaact aaagatatac aactgcatca gggaggcagc agatcaactt
      601 ccggcatggt tcccattagc tatggattta gcccttgtaa caggacaacg tcgcgaagac
      661 ataacgaata tgcggttcag tgatatttat gatgatcgtc tccacgtcag gcaaattaag
      721 acaggaatga tgattgctat ccccctgtca ctcagccttc ctgtcgctgg tctacggctt
      781 ggtacagtag ttgaacgatg ccgcctggta agccggggag attatctaat cagtgccggg
      841 attagaaaaa acagccctga cggcagcatt cacccggatg gcctgacaaa aaaatttgtc
      901 gcagccagaa aattcacagg tatccagttc agtgaaaacc caccaacttt tcacgagatc
      961 agaagcctgg ctggacgatt gtacaaagaa acatgtggag aagaatttgc tcagcgtcta
     1021 cttggccaca catcggagaa gacaacaaaa atgtatcttg atgagagaga aaaaacgtac
     1081 ttactgctct gattttaacg taaatggatt gttaaatgta ttttggttgt gatataacca
     1141 aaaagaccgg aatacagaaa ttcgagtaaa tttcggggaa tttcggggga gacatttgca
     1201 actaattgat ttcaaatgca attaaaaaaa gaccgaatac gattcctgta ttcggtccag
     1261 ggaaatggct cttgggagag agccgtgcgc taaaagttgg cattaatgca ggcttagttg
     1321 ccttgccctt taagaataga tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca
     1381 ataaaaagcg cggtggtcat cagcttaaat gttaaaaacc gcccgttctg gtgaaagaac
     1441 tgaggcggtt tttttattgg aaatcaaaag gctattttag gtaattaaca gagtttttca
     1501 gctcgttcta taaacggtgc cagactcatt ttttcgccgg gattgttagg atcatcaatc
     1561 tgaatcaccg aaatgggttg ggcattggtc ttcccactgg caacttcctt ttgtgcgata
     1621 tcgtttaaag gatactgcac gagggtactc ggattaataa catacaaagc attacccggt
     1681 cggcaagtca gcatcacctc ttcgcgatta aacgcccatt tgtctttacc cacttcaaaa
     1741 cgactgacgg taatcacctg cggtgcagcc agcgccgctg cagaactggt gagtaacaga
     1801 aacgccagaa tacttttttt catcattttc gtaatccatc aaaaaggttc gagagtcgca
     1861 agcaggctga tgatcaccag cactaccgca cctatcgccc attcgagttt agtcatccac
     1921 acaaaccagg gtgccgcacg atcttcctcc tgcctcatgc gcggtacaag aacataacga
     1981 ttagccagcg caatgaccac cataatcatc accaggatgg ctttgagcaa taagagttgg
     2041 ccccagtaag tcgtaagcgt ggggggaaat ccggtaatca acaatgcatt aagcacgccg
     2101 cttgccagta cgccgatcac cgcaaaatgc ccgcaccatg aaaaacacat cagcgcctga
     2161 atagcctggt gtcgccaacg gcctttgatg agttgcatac accagagcac cggcagtaat
     2221 cccccaaacc acgccgccgc acaaatcagg tgaatcgcgt gattagtctg atggattttc
     2281 gccgttaccc cttcattcag tgtcgcatgc cccattcccg ccagcagaat aaattgcgcg
     2341 gtagtgagca taaacagcaa acgtggcata ttccgcggtt gcataagggc gacgatcaac
     2401 gttacgaggg cgagaacaat ttgccataac cagacgccac caaactgcgt ttgcaaaaca
     2461 gcctgccaaa tgtttggcga aaatacatct gtccatcccg ttcccatcaa cccgccctga
     2521 acggcgagca ttgcagtggc actgatcaaa ctccagacgg cagcgtgctg ttgtaaacgc
     2581 agaaaacgct tcgccagcaa gcgacgaatc gtcaaggggg ccagccaggc accgtacatt
     2641 gcgaagccaa aaaccagcat cagcgaggtg aaatggataa atcgcagcgc gatccaggta
     2701 aacgccagca tgatttattt cacgctaaag gtgtagtgtc ctttcgtttt atgcccgtcc
     2761 accgaaacaa catgccagtc aacggtgtag gttccgggtt tcagcgaatc ggccagtgga
     2821 acaatcagtt gcttttggtc ctgctcattt cgcttcgccg gtaatgtttt aatattttcg
     2881 ttttttggcc ccgtgatttt tgcaccactg aatcctgttt ccacaccttc cgagaagttt
     2941 aaagtgattg cctgcggtgc agctgtcact tgcgcgtttg ccgcaggata ctgatgcgtt
     3001 aaatgcgcat gtgcccagac gaaaggtgtc accaacgaag tggtcaggat tgccagcgcg
     3061 tagcgaaggg agcgtgcagt tgaagccata ttatctattc ctttttgtaa taactttttt
     3121 acagagcata accttgtcta atgtctgagt cgaggatcat caattccggc ttgccatcct
     3181 ggctcactct tagtaacttt tgcccgcgaa tgatgaggag attaagaatg ctgaagaatc
     3241 tggctaaact ggatcaaaca gaaatggata aagtgaatgt cgatttggcg gcggccgggg
     3301 tggcatttaa agaacgctac aatatgccgg tgatcgctga agcggttgaa cgtgaacagc
     3361 ctgaacattt gcgcagctgg tttcgcgagc ggcttattgc ccaccgtttg gcttcggtca
     3421 atctgtcacg tttaccttac gagcccaaac ttaaataaaa cttatacagg gttacacttt
     3481 cttacataac gcctgctaaa ttatgagtat tttctaaacc gcacccataa tttgcagtca
     3541 ttttgaaaag gaagtcatta tgtcgttctg gaaagttgca gcagcgcaat atgagcctcg
     3601 taaaacctca ctcaccgaac aagtagctca tcatctggag tttgtgaggg ccgccgccag
     3661 gcaacaatgt cagctgttag tttttccatc gctttcttta ctggggtgtg attattcgcg
     3721 acgtgccctt cctgccccac ccgatctctc actgttagac ccgctttgtt atgccgcaac
     3781 gacctggcga atgaccatca ttgccggcct tcccgttgaa tataacgatc gctttattcg
     3841 tggaattgcg gtgttcgcac cgtggcgaaa aacgcctggg atctaccatc agagtcatgg
     3901 tgcatgtctg ggccgtcgtt ccagaaccat cacggtggtc gatgaacaac cggaaggtat
     3961 ggatatggac ccgacctgtt cactgtttac gacagggcaa tgtctgggag aacccgacct
     4021 gctggcgtcg gcccgtcgct tacaattttt ttcacatcag tactcgattg ctgtgctgat
     4081 ggcaaatgcc cgtggtaaca gtgcactatg ggatgaatat ggtcgtctca tcgttcgcgc
     4141 cgatcgtggt tcattattgt tagtcggtca gcgttcatca cagggttggc aaggcgatat
     4201 cattccatta cgctaggctt tttcggcctg gagtatgcca tgttgcgcat tatcgataca
     4261 gaaacctgcg gtttgcaggg agggatcgtt gagattgcct ctgttgatgt cattgacgga
     4321 aaaatcgtca accccatgag ccacctggtg cgccccgatc gtcctattag tccacaagcg
     4381 atggcgattc atcgcatcac cgaagccatg gtcgccgata aaccgtggat tgaagatgtg
     4441 atcccacact attacggcag tgaatggtat gtcgcgcaca acgccagctt tgaccgccgc
     4501 gtactgcctg agatgcccgg tgagtggatt tgcaccatga aactggcccg tcgcttgtgg
     4561 ccaggaatca agtacagcaa tatggcgtta tataaaacac ggaagctcaa tgtacagacg
     4621 ccgccgggcc tgcatcatca ccgcgcgttg tatgactgtt atatcaccgc cgcgttgctt
     4681 atcgatatta tgaacacctc cggctggacg gcagaacaga tggccgatat caccggacgt
     4741 ccgtcgttga tgacgacctt cacctttggc aaataccgtg gcaaagcggt ttccgacgtt
     4801 gccgaacgcg atccgggcta tctgcgctgg ttatttaata acctggacag catgagcccg
     4861 gagctgcgtt taacactgaa acattatctg gaaaatactt aatccgcagg cgtagcgggt
     4921 aatgttccct gcgccagcgc gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat
     4981 ttaaagcggc cagatttacc gccatggcct gagtccatgt cggtacagag caataaaaga
     5041 tggttatcgg ttttcagctc gcgcaattta gcgacccatt ttgccggttc ccaatattgc
     5101 acctgagaat cgtgtaaacc ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg
     5161 ttgtcatacg ggctgtagct tttcatgtac tcgtaatatt gcggatcctg cgggttaccc
     5221 cactcttcaa actcaccagt ggtaagagga attgattcat caagcatcgt tgttacaaca
     5281 tcaacaaacg gtacctgggc gataacgccg tggaataatt ccgggcgttg attaattgca
     5341 acgcccatca acatgccccc cgcactcccg cccatcgcat aacaaagcga aggagagcca
     5401 tagcccagtt ttaacaatgc atcgcaggca tcaagataat cattaaacgt atttttcttc
     5461 ttcagaaatt ttccgtcttc gtaccattgt tgccccagct caccaccgcc gcgaacatgg
     5521 acaatggcgt agacaaagcc acgatctaac aaactcaagc ggctaaaact gaaatcggca
     5581 tcaatacttg cgccgtaaga accatagcca tacaccagca acgggttgtg tcctttgcga
     5641 aaatgtttgc gatggtagac caacgaaacc ggaacttcga cgccatcacg ggcgactatc
     5701 cacaggtgtt cactgcggta attcgccgca tcaaaaccag gaacttccgt ttgttttaat
     5761 acacgacgct catcggtatc catatccagt tcaaacaaag tgtctggtgt ggtcatggaa
     5821 gaataaccat aacgcaatcg cgcggtttca ggttctggat tgtaggcaat ccaggtcaca
     5881 taggctggat catcaaaggc aataccaatg acttcccggg tcttgcggtt aatttggcgc
     5941 aaactggtta acccgcgctg acgctcttca accaccagcc agtcggtaaa cagcgtaaac
     6001 ccttccagca tgatgttttc gcatggcgga attaactctt cccactgttg ctcatcacgc
     6061 atacgggtac ggtataagcc aaagtttttg ccgtggcggt tggaacgcag ataaaaacga
     6121 tgctggtagt gatcaaggct gtattcgtga tctttgcggc gcggcagaaa aacaaacggc
     6181 tcggcatcgg ccatttccgc gtccagcagg cgaacttcac tggtggtggc gctggccaaa
     6241 tgaatgacta catagtgctt cgaggtcgtt ttatgcaggc tgacgtaata ggtatcgtct
     6301 ttttcttcgt agatcagttt atcttgcgat gctggcgtac cgatggcgtg acgccagacc
     6361 tgataaggca gcagcgtcac cggatgcttg cgaacatagt agaaagtcca ggagtcattt
     6421 gcccagacaa agctgggttc aacgttatcc agcagttccg ggtaccagtt accagtttcc
     6481 agattacgaa aacgaatgcc gtactggcgt cgggaaagaa aatcttctgc cagcgccata
     6541 atggtgttat cgggcgtaat cgccattccg cccatcgaat aaaactcact atgagctgcg
     6601 cgcttattgg catcgagcaa tatttcccac tcatcccact cttcactgaa tgccgattga
     6661 cgctggtaga tagcatattc acagcctggt tcataaatat gccgatagcg gtagccattt
     6721 ttgatgtagg gcgcagaaac ttctcgttgc ggaatgcggt cgatgatttc ctttaagatg
     6781 cgatcctgca aggcttgttg tgaggccatc acccgatgac cgtaactatt ttcttgttgc
     6841 aggtagtcca ggacttctgg ctgagaacgc gtatcatccc gcagccagta gtaattatcg
     6901 atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc gggcggcttt tggtagcatg
     6961 ttattgttct ttctggttga aacatcttat aagggtggca aaactcaccg gggatgcaag
     7021 cgaaacaggg gagtcattgc ttagatgatg acaggtaatg gcacggatat cgaatgttat
     7081 gcagacagag aaactcagcc tgttcaaatg gctgtgcggt tctggatagc ccgaaatagt
     7141 caacttcagg ctatccagag agcggaatta ttccgccaaa gtgcgttttt gctgttcgag
     7201 atcgcgttca atgccgtcac gaacatcctg ggggattttc agcgcgtcac ccagtgcatt
     7261 caggtaactg cgttccataa aatggtcaat atcaatagcc gcgcaactca ggaaatagat
     7321 ttccagcgcc tcttcttcat tgcggacccc ggtagccagg cgttgtggat ccagcggttg
     7381 ttcgattgcc tgctcaatga gtacacgccc ctgctcttcc acgccggctt cacgcaattg
     7441 ctggtcgatt gccgcacgtt ctttggcatc aatatgacca tcacttttag cggcaaagac
     7501 cagcgcaagg atcaaacgtt ctgtacgctc atccagcggc gtactttgcg cgccaaactg
     7561 cggttcgtcc tgatgcgccg cgcgaatttt atctttgtat ttattccaca gcaccgtacc
     7621 cgctaccgct ccgccgccaa ccagtaacgc gttggtgcca tatttcgtaa gaagtttacg
     7681 tgctgattta tttgcgacca gcagcccagc cagtccgcct aatgcgcctg gcactaacag
     7741 tttgcccaat ccctgatccg cagacgagga ggtagaagaa ctgctttgcc caagcaggga
     7801 ttgcagttga tttaaccagt tagccatatt tgctcctcaa taaccatttt tagtgcctgt
     7861 agcatagcgg atgaggatgt cagaaaatgt atatcgagac aaaagatgcg caaataagcg
     7921 gggcggtagg ccgttattca aagaaattcg cgccaggtaa ggtatttact gtatatcagg
     7981 tgataagggt attttaggtg aaagtagttt gtcgttttga gtggcattgt ttgatgctga
     8041 ttgcccgatg cgacgctgtt gcgtcttctc aggcctacga ttgtggtgcg ttctggggcg
     8101 gacaaggcac tcgcgccgca tccaccaacg gtcaatcgaa ttatcagaag ccattaacgc
     8161 cgctgatatt ccgccattcc cgctttgcag tccacgctga cctggtaatg aatatcggca
     8221 cttttaccac gcacggttag cggtactgac catttatcat ctttaccctg aatgtcctgc
     8281 aaactgaccc aagccacggg atcggcctga ccgacaattt tttgatcatc tgcccaacgc
     8341 gccacgcgat tttgttgata atcacgtttt acgctcgcgg caattccggc agcatccaga
     8401 ccttcacact ttgggaaagt gaccgacttg ctggtttcat tattggcagc gaaaactgat
     8461 gcgcaggcag aaaccaacaa cagccctaaa aacgcccctc tttttttcat gtttttctcc
     8521 atagcacaat gattcaggag aaagcatggt acaaattgtc aggagcgcaa gttgcttcag
     8581 gccgcgtgtg aggcatcttc ctcttcggat tcagcaccga cgacctgtgg ggacggtaat
     8641 ttgccggttt tcagaatggt gctcagcaca tctttttgtt ctgccagcca tagcgaaagg
     8701 gcttcacgtt gctcgtcttc catttgcact ggagaatttg tcagccaggt gtcaagcaga
     8761 tccgccgtat caagcatttt gtcataagca tcggcttcct ttttgctggt aaacgacatt
     8821 ttctcttcgc cctcacgaat gactacgtat ttaacttcaa ccgccatttg cagcctctca
     8881 taataactgt gattttatac agtatatttc ttttcggttg agaaatcaac atcagcaata
     8941 aagacacacg caaacgtttt cgtttatact gcgcgcggaa ttaatcaggg gatattcgtt
     9001 atgacgttat taggcactgc gctgcgtccg gcagcaactc gcgtgatgtt attaggctcc
     9061 ggtgaactgg gtaaagaagt ggcaatcgag tgtcagcgtc tcggcgtaga ggtgattgcc
     9121 gtcgatcgct atgccgacgc accagccatg catgtcgcgc atcgctccca tgtcattaat
     9181 atgcttgatg gtgatgcatt acgccgtgtg gttgaactgg aaaaaccaca ttatatcgtg
     9241 ccggagatcg aagctattgc caccgatatg ctgatccaac ttgaagagga aggactgaat
     9301 gttgtcccct gcgctcgcgc aacgaaatta acgatgaatc gcgagggtat ccgtcgcctg
     9361 gcggcagaag agctgcagct gcccacttcc acttatcgtt ttgccgatag cgaaagcctt
     9421 ttccgcgagg cggttgctga cattggctat ccctgcattg taaaaccggt gatgagctct
     9481 tccggcaagg ggcagacgtt tattcgttct gcagagcaac ttgctcaggc atggaagtac
     9541 gctcagcaag gcggtcgcgc cggagcaggc cgcgtaattg ttgaaggcgt cgttaagttt
     9601 gacttcgaaa ttaccttgct aaccgtcagc gcggtggatg gcgtccattt ctgtgcaccg
     9661 gtaggtcatc gccaggaaga tggcgactac cgtgaatcct ggcaaccaca gcaaatgagc
     9721 ccgcttgccc ttgaacgtgc gcaggagatt gcccgtaaag tggtgctggc actgggcggt
     9781 tatggtctgt ttggtgtcga gctgtttgtc tgtggtgatg aggtgatttt cagtgaggtc
     9841 tcccctcgtc cacatgatac cgggatggtg acgttaattt ctcaagatct ttcagagttt
     9901 gccctgcatg tacgtgcctt cctcggactt ccggttggcg ggatccgtca gtatggtcct
     9961 gcagcttctg ccgttattct gccacaactg accagtcaga atgtcacgtt tgataatgtg
    10021 cagaatgccg taggcgcaga tttgcagatt cgtttatttg gtaagccgga aattgatggc
    10081 agccgtcgtc tgggtgttgc gctggcgacc gctgagagtg ttgttgacgc cattgaacgc
    10141 gcgaagcacg ccgccggaca ggtaaaagta cagggttaaa cccggcaaaa aaacgctaca
    10201 aaaatgcccg atcgtagatc gggcgttttg acttttacag cttagcgcct tctacagctt
    10261 cacgcgccag cttagtaatg cggtcgtaat cgcccgcttc cagcgcatct gccggaacca
    10321 gccaggaacc accgatgcac agcacgcttt tcagcgccag gtagtcacgg tagttagccg
    10381 gagaaatacc acccgtcggg cagaaacgga cctgggagaa cggacccgcg atcgcctgca
    10441 gggctttcac gccgccgtta gcttcagccg ggaagaattt gaactctttc aaaccgtagt
    10501 ccatacccag catcagttcg gaaacagtgc tgatccccgg aattagagga atagtccctt
    10561 cggtagcagc tttcagcagc ggctcggtca gacccgggct aattgcgaac tgtgcacccg
    10621 cttcagtgac ttctgccagc tgctgtggat tcagcaccgt accggcaccc acaatcgctt
    10681 caggcacttc tttggcgata gcacggatag cgtcaactgc acactcggta cgcagagtca
    10741 cttccagaac gcgcacccca ccagcaacca acgcttttgc catcggcact gcgtgttcca
    10801 gtttttttac cacgataacc ggtacaaccg ggccggtggt caggattgat tctgcacttg
    10861 ttttccagtt tttcatcaga gttttctctc gcctgattac aaatttgtcg tcttaaaaag
    10921 tgatacaggt tgcgccctgt tcggcaccgg acagtttttc acgcaaggcg ctgaataatt
    10981 cacgtcctgt ccccacgcgt gacgcgctca ggtcaggaat gtgcggttcg cgagcagcca
    11041 gttccgcttc gtctaccagc agcgtcagtt cgcctgtctg tccattcaca cgaatgatgt
    11101 ccccgtcgcg cacttttgcc agcagcccgc catcgtaggc ttctggtgtt acgtggatag
    11161 ctgacggcac tttacctgaa gcgccggaga gtcgtccatc ggtaactaac gcaattttga
    11221 aacaccggtc caataataca ccaagtggcg gcatgagttt atgtaattct ggcattccgt
    11281 tcgcttttgg cccctgatga cggacaacaa cgacacaatc gcggtccagc aaacccgctt
    11341 caaaggccgg cataacgtca tgctggcttt caaaaacaac cgctggcgct tcaatcacct
    11401 ggttctcaac cggcacggca gaggttttca taaccgcacg gcccaggtta ccgcttaaca
    11461 cttttgtccc accatgatga gagaaaggtt gttcgaagga agcgatcaca ttgctgtcga
    11521 gtgatttttc cgccccttcc cgccagtcct gttcaccatt attcagccat ggttcaaggg
    11581 tataacgaga cagaccaaag cctgccaccg tattgacatc ttcatgcagc aggcctgctt
    11641 tgagcagttc acgcaccaga accggtacgc cacctgccgc ctggaagtgg ttaatatcgg
    11701 ccggaccgtt cgggtagaga cgtgccatca gcggtacaac atcagaaagg tcagagaagt
    11761 catcccagtt aatctgaata ccggccgcgc gcgccatcgc caccaggtgc atggtgtggt
    11821 tagtggaacc accggtcgcc agcagtgcaa cgataccgtt caccaccact ttctcatcga
    11881 tcatcttacc gatcggcatc cattcattac cattaccggt catgcgtgta acctgacgcg
    11941 cagcggcagc ggtcaaagca tcgcgcagcg gagaatccgg atgaacaaaa gaagagcctg
    12001 gcaactgcat ccccataaat tccaccacca tctggttggt gttggcagta ccgtagaaag
    12061 tacatgttcc cggcgcatgg taagacgcgg cttctgactc cagtaaggcc atgcggtcca
    12121 ctttaccttc ggcataaagc tggcgaatac gcactttttc tttatttggc aaaccgcttg
    12181 ccatcggtcc agacggcaca aacaccgcag gcaaatgacc aagcgacagg gctgccatcg
    12241 tcagacccgg gacaatcttg tcgcacacac cgaggaacag agcaccatca aacatgttat
    12301 gggacagccc caccgccgca gacatcgcta tcacttcgcg gcttagcagc gacaattcca
    12361 ttccatcctg cccctgggtg acaccatcac acatcgccgg aacaccgccc gcaacctgac
    12421 caaccgcatt cgcttcatgc agggctttac gaatgatttc tggatagtgt tcataaggct
    12481 ggtgcgcgga gagcatgtcg ttataggagg tgatgatggc gatattgtta cgcaacatgc
    12541 ttttcaaaga ggctttgtct tctggctggc aggcagcgaa accgtgtgcc aggttaccgc
    12601 atgccaactg cgaacgatga acggtcgacg ttttcgcttg ttctatccgg gcgagataag
    12661 cagagcgagt ctcgcgcgaa cgttcaatga ttcgatttgt tacgcataac aattgtggat
    12721 tcataaaggc tcctgaaatt gagttgtcag agcaggatga ttcacaacgc gtttcattca
    12781 gaggatttat gagtgaaacg cctgtaaccg gagctcatag ggcaaaaacg tttcagtcag
    12841 tgtaataaaa aaagcctcgt gggtgaatcc gcacgaggcc tgaaagtgta aaaattgttc
    12901 tacaatctgt gcaagatcat gttaccggta aaataaccat aaaggataag cgcagatatt
    12961 actcaaactc attccaggaa cgaccatcac gggtaatcat cgccaccgag gcaacgggtc
    13021 cccaggttcc ggcctgatac ggtttcggcg catcattgtc catcgcccac gcctcagtaa
    13081 tggagtctac ccatttccag gcttcttcca cttcatcacg acgtacaaac agtgcctgaa
    13141 taccacgcat ggtttccagc agcagacgtt cataggcatc cgccagatgc gtctgattaa
    13201 aggtttctga atagctcaga tccagcttgg tgatttgcag gttatgtttg tggtcaaggc
    13261 caggaacttt attcagtacc tggatatcca cgccttcatc aggttgcaga cggatagtca
    13321 gtttattctg cggcagatcc tgccacgact ctttaaacag attcagttca ggtgttttga
    13381 aatagaccac gacttcagaa catttggtcg gcagacgttt accagtacgc aggtagaatg
    13441 gcacaccggc ccagcgccag ttatcaatgt cgacgcggat cgccacgaag gtttctgtat
    13501 tgctgctctt gttcgcgccc tcttcttcca gatatcccgg cacttttttg ccctgggcga
    13561 agcccgcagt atattgcccg cgtacggttt tttcgcgtac gttggagcgg tcgatgcggc
    13621 gcagggactt cagtactttc actttttcat cgcggatgct gtctgcgctc aggtcagatg
    13681 gcggagacat cgcaatcatg cagagaattt gtagcaggtg gttctgaatc atgtcacgca
    13741 tctgaccggc tttatcaaaa tagccccagc gcccttcgat ccccacttct tctgccacgg
    13801 taatctcaac atgatcaatg gtgcgattgt cccagttatt cacaaacagg gagttagcaa
    13861 aacgcagcgc caacaggttc agcaccgttt ctttaccaag atagtggtcg atacggtaaa
    13921 cctggcactc ctcgaagtat tcgccaacct gatcattgat ttcctgcgag gtcgccagcg
    13981 acgtccccag cggtttctcc atgactacgc gtgccggttt agcattcagt tttgcctcgc
    14041 caagcccttt gcaaattgcg ccaaaagtgc tgggcggcat ggcaaagtag ttaatggtga
    14101 tacgattttt ttgatccagc atcgcgccga gacggttgaa tgcagcagtg tcattgacat
    14161 cgagattaca aaaatccaga cgtgcactca gggtgtccca taaaccttca tcaatggttt
    14221 ctttcatgaa agtttcgagc gcctcgcgga caactttggt atatgccgct ttatcccagt
    14281 cagcacgccc tacgccgata atccgggtgt ccgggttgag ctgaccggct ttttccagtt
    14341 gatacaggga aggcagcaat ttacgacgcg caaggtcgcc tttcgcgccg aaaatgacca
    14401 ggtcacaggc ctgggctgtt tgcgttaccg ccatgtcatt ctccttaagt taactaaccc
    14461 ggtacttaag ccagggtata cttgtaattt tcttacggtg cactgtactg cttttacgag
    14521 cttgcgaaaa ctgtaaacgc ttatccaccc gtgcgattac gggaaaagcg cgcaaagtgc
    14581 ggcaaaactg ataaaaaaat catcgttttc cattcggtaa aaatctgaca ctgatcatgt
    14641 tatgaaaaaa aataacaact tttttatctg cttttgtcat taacacggca cacaggcgta
    14701 atatctgaca aaactgcatt tcgatttctt tcagtgcgga aatcgtcatt acccgtgagt
    14761 ctctttacat catgaatatg ctggaaaaaa tccagtctca gctggaacat ttgagcaaat
    14821 cagagcgcaa agttgccgag gtcattctgg cttcgcccga taacgcgatc cattcgagta
    14881 ttgctgctat ggcactggaa gccaatgtta gcgaaccgac ggtgaatcgt ttctgtcgca
    14941 gcatggacac gcgcggtttt cctgatttta aacttcatct ggcacagagt ctggcgaatg
    15001 gcactcccta tgttaatcgc aatgtcaatg aagatgacag cgttgaatca tacacaggga
    15061 aaatatttga gtccgcaatg gcaacgcttg atcatgtccg tcattcactg gataaatctg
    15121 ccatcaaccg caccgtcgac ttgctcactc aggcaaaaaa aatcgccttt ttcggattag
    15181 gctcttcagc cgccgttgcc cacgatgcga tgaataagtt ctttcgtttt aatgttccgg
    15241 tggtgtactc cgatgatatc gtgctgcaac gcatgagttg tatgaattgt agcgacggag
    15301 acgtggtggt gctgatttct cacactggaa gaacaaaaaa tctggtcgag ctggcgcagc
    15361 tggctcgcga aaacgacgca atggtgattg ccctcacctc tgcgggtacc ccgctcgccc
    15421 gggaagcaac gctggcaatt accctcgacg taccggaaga tactgacatt tatatgccca
    15481 tggtttctcg acttgcacag ctgaccgtga tagatgtgct ggcgacagga tttactttgc
    15541 gacgcggtgc aaaattcaga gataacttga agcgggtcaa ggaagcgctg aaggaatcgc
    15601 gttttgataa gcagttactt aatttaagtg acgatcgcta aaaacgactg tcactgtcct
    15661 aatcttatac gacatccgaa tgagattaat ttatcgccat cgcggcgtta tttcattcgg
    15721 atttcatgtt caagcaacac ctggttgttt cagtcaacgg agtattacat gtccagaagg
    15781 cttcgcagaa caaaaatcgt taccacgtta ggcccagcaa cagatcgcga taataatctt
    15841 gaaaaagtta tcgcggcggg tgccaacgtt gtacgtatga acttttctca cggctcgcct
    15901 gaagatcaca aaatgcgcgc ggataaagtt cgtgagattg ccgcaaaact ggggcgtcat
    15961 gtggctattc tgggtgacct ccaggggccc aaaatccgtg tatccacctt taaagaaggc
    16021 aaagttttcc tcaatattgg ggataaattc ctgctcgacg ccaacctggg taaaggtgaa
    16081 ggcgacaaag aaaaagtcgg tatcgactac aaaggcctgc ctgctgacgt cgtgcctggt
    16141 gacatcctgc tgctggacga tggtcgcgtc cagttaaaag tactggaagt tcagggcatg
    16201 aaagtgttca ccgaagtcac cgtcggtggt cccctctcca acaataaagg tatcaacaaa
    16261 cttggcggcg gtttgtcggc tgaagcgctg accgaaaaag acaaagcaga cattaagact
    16321 gcggcgttga ttggcgtaga ttacctggct gtctccttcc cacgctgtgg cgaagatctg
    16381 aactatgccc gtcgcctggc acgcgatgca ggatgtgatg cgaaaattgt tgccaaggtt
    16441 gaacgtgcgg aagccgtttg cagccaggat gcaatggatg acatcatcct cgcctctgac
    16501 gtggtaatgg ttgcacgtgg cgacctcggt gtggaaattg gcgacccgga actggtcggc
    16561 attcagaaag cgttgatccg tcgtgcgcgt cagctaaacc gagcggtaat cacggcgacc
    16621 cagatgatgg agtcaatgat tactaacccg atgccgacgc gtgcagaagt catggacgta
    16681 gcaaacgccg ttctggatgg tactgacgct gtgatgctgt ctgcagaaac tgccgcaggg
    16741 cagtatccgt cagaaactgt tgcagccatg gcgcgcgttt gcctgggtgc ggaaaaaatc
    16801 ccgagcatca acgtttctaa acaccgtctg gacgttcagt tcgacaatgt ggaagaagct
    16861 attgccatgt cagcaatgta cgcagctaac cacctgaaag gcgttacggc gattattacc
    16921 atgaccgaat cgggtcgtac cgcgctgatg acctcccgta tcagctctgg tctaccaatt
    16981 ttcgccatgt cgcgccatga acgtacgctg aacctgactg ctctctatcg tggcgttacg
    17041 ccggtgcact ttgatagcgc taatgacggc gtagcagctg ccagcgaagc ggttaatctg
    17101 ctgcgcgata aaggttactt gatgtctggt gacctggtga ttgtcaccca gggcgacgtg
    17161 atgagtaccg tgggttctac taataccacg cgtattttaa cggtagagta atctcacctg
    17221 ccccggccgc tgatggccgg ggtatttagc aacacataaa ttaggactac ttattcaatc
    17281 caatcataat cacagccccc tgtaaaatga atacagggat agcctgaatg gagatctaaa
    17341 tagcttgcaa gacgtcgcat acccgcttcc tctgcaacac tatgtccaat aactgccatc
    17401 ggtaagccac tatctttgga gtattgtgtc tgcacccatg ttttaattga atcgttaatc
    17461 gtaatataat aatcagcctg atattccata tattgtattg gatcacaaaa acagcctgcc
    17521 ccaatgccaa cagttctgac tactctggct ttatctccat aaaattgaat gccaggaaga
    17581 ttatatattt tcaaattttt ggcaaaacgt ttggtaatgt taatggcact gtcagggtct
    17641 atagcatata cacagtacat ctcatcagaa gcaacaatat ttgtaacatc cagttctaac
    17701 tgctgagcca aagccttaga cattccgaac gtaggctctc gatccatgac atcatggcaa
    17761 cgaatgatta ccaaattatt tttatgcaaa aactcctttt tttgctcaat tatttttaaa
    17821 taactgagtt caccgcgagt ataatccatt atttttctgg cccatgaaaa ctcaagatcg
    17881 tcaccctcca gatcagcata agaataaaat gtaggctcat gtgtaataat gatattgaca
    17941 ccttcaaaaa cagcctttct taaaacatcc attgaggcaa tccagcaggt cccaatttta
    18001 ttaataagtg tcgcctcagc accaaaaata attttatcaa cagttggttg cgaaacagca
    18061 accattgaat ttaaaaaaca attaagatcc gcggctgttt taatatgatg cattcaacat
    18121 ccttcacagg tttcatcagc aataaaataa tttcaacatt tctgggaaac gttttgccca
    18181 tgcagactca gagtgggtat cacctcccca taaatg
//
LOCUS       DAJTVJ010000085        18187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000085 DAJTVJ010000000
VERSION     DAJTVJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.085"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..871)
                     /gene="adrA"
                     /locus_tag="OO448_002826"
                     /gene_synonym="dgcC"
                     /pseudo
     CDS             complement(26..871)
                     /gene="adrA"
                     /locus_tag="OO448_002826"
                     /gene_synonym="dgcC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
     gene            complement(973..1293)
                     /gene="psiF"
                     /locus_tag="OO448_002827"
     CDS             complement(973..1293)
                     /gene="psiF"
                     /locus_tag="OO448_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6337995.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1412..2827)
                     /gene="phoA"
                     /locus_tag="OO448_002828"
     CDS             complement(1412..2827)
                     /gene="phoA"
                     /locus_tag="OO448_002828"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6337996.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2928..3188)
                     /gene="iraP"
                     /locus_tag="OO448_002829"
     CDS             complement(2928..3188)
                     /gene="iraP"
                     /locus_tag="OO448_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6337997.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3370..3573)
                     /locus_tag="OO448_002830"
     CDS             complement(3370..3573)
                     /locus_tag="OO448_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6337998.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3651..4745
                     /gene="ddlA"
                     /locus_tag="OO448_002831"
     CDS             3651..4745
                     /gene="ddlA"
                     /locus_tag="OO448_002831"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6337999.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4769..4981)
                     /locus_tag="OO448_002832"
     CDS             complement(4769..4981)
                     /locus_tag="OO448_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6338000.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5241..5549
                     /locus_tag="OO448_002833"
     CDS             5241..5549
                     /locus_tag="OO448_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6338001.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5608..6702)
                     /gene="yaiW"
                     /locus_tag="OO448_002834"
     CDS             complement(5608..6702)
                     /gene="yaiW"
                     /locus_tag="OO448_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6338002.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6715..7935)
                     /gene="sbmA"
                     /locus_tag="OO448_002835"
     CDS             complement(6715..7935)
                     /gene="sbmA"
                     /locus_tag="OO448_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6338003.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8287..9444
                     /gene="ampH"
                     /locus_tag="OO448_002836"
     CDS             8287..9444
                     /gene="ampH"
                     /locus_tag="OO448_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6338004.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9445..10068)
                     /gene="iprA"
                     /locus_tag="OO448_002837"
     CDS             complement(9445..10068)
                     /gene="iprA"
                     /locus_tag="OO448_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR6338005.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10156..13044)
                     /locus_tag="OO448_002838"
     CDS             complement(10156..13044)
                     /locus_tag="OO448_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6338006.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13570..14544
                     /gene="hemB"
                     /locus_tag="OO448_002839"
     CDS             13570..14544
                     /gene="hemB"
                     /locus_tag="OO448_002839"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6338007.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14650..15501)
                     /gene="tauD"
                     /locus_tag="OO448_002840"
     CDS             complement(14650..15501)
                     /gene="tauD"
                     /locus_tag="OO448_002840"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6338008.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15498..16325)
                     /gene="tauC"
                     /locus_tag="OO448_002841"
     CDS             complement(15498..16325)
                     /gene="tauC"
                     /locus_tag="OO448_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6338009.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16322..17089)
                     /gene="tauB"
                     /locus_tag="OO448_002842"
     CDS             complement(16322..17089)
                     /gene="tauB"
                     /locus_tag="OO448_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6338010.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17102..18064)
                     /gene="tauA"
                     /locus_tag="OO448_002843"
     CDS             complement(17102..18064)
                     /gene="tauA"
                     /locus_tag="OO448_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6338011.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4534 a         4728 c         4395 g         4530 t
ORIGIN      
        1 tatcaataag ttggagtcat tacccgctac cgcgcagggt aatttgtaac tgtcgggtaa
       61 gcgccacaat cgcttcatcg cccacatcat gaccccaggt atcgttgatg ctcttgaaat
      121 ggtcgatatc gataatcagt aacgttgcat cgcgattatg ccgccgacag ttatcaaatt
      181 cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcatg ccgtcgcgag
      241 tactcatgac ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc tggtagctga
      301 cccagccaaa cagcagagga taaacgacaa taatgggaag ggagagccac cattccagcg
      361 gcgcactatt gaacgacacg gtaatgcccg taagctcgag ggtgacaagg caggaaacca
      421 ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc aaattcagac
      481 acataatcat caacatagcg gtggaaggca gcacgtttac gcccattacg cctacccaca
      541 ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg
      601 ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg accaacacca
      661 gccaccacca gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc
      721 cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct
      781 gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg cgccgccgct tttttgaaaa
      841 agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa attatagaga
      901 cggggctaaa ggcagaagta tgaaatttcg ggtgagccac tttcgtagct cacccgttag
      961 cactatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
     1021 acctttcaga gattgttgcg ttgcttgatt attacattcg cgcatctttt gctgctgtgg
     1081 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
     1141 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
     1201 ctgttgttgt ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc
     1261 aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
     1321 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
     1381 cggcagcgaa aattcactgc cgggcgcggt tttatttcag ccccagagcg gctttcatgg
     1441 tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc ggaccatacg
     1501 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcggaa ttcccgtaac
     1561 tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg
     1621 tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
     1681 taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
     1741 cgccaatttg cccacaagga ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa
     1801 cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggttttg tcggtcatct
     1861 gtgccagggt tggtacactg tcattacgtt gcggattagg cgtacaggtg actgcgggct
     1921 tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt
     1981 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
     2041 cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     2101 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
     2161 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
     2221 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg
     2281 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     2341 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     2401 ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc ggttgctgat gcagccgagt
     2461 cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg
     2521 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     2581 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     2641 gtttatcgct aagagaatca cgcagagcgg cggtctgatc actcgttaag cggcgagcac
     2701 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
     2761 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
     2821 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
     2881 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacgagga
     2941 tgcttcaata acttctttac gtaatcgcgc agcagctccg tatcgtcgtc aggaatgctg
     3001 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
     3061 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
     3121 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga
     3181 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac
     3241 ttcatagtgt ctggaagtat tatttttatg acaaaggaaa tactttgcgc aaatattttt
     3301 tgtttgatta ccagcgaaac gtttctctta tttaaataat tacccacaca aaatataggc
     3361 ttttaattat caacctttat tataaataaa agttatgaaa tatattctct tcgttaactt
     3421 ataattaaga gaaaaaaact caccgcattg ccatttaagc caggaataaa ttagcttccc
     3481 gttcatcatt tttgtgatct cgcgcacatc tttgagataa cgcactttct tcaccagtac
     3541 gttgcacgct ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt
     3601 tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac
     3661 tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg
     3721 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg
     3781 ataaacaagg gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc
     3841 ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg
     3901 agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc
     3961 cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca
     4021 atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca
     4081 ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg
     4141 ctaatcgtca caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg
     4201 taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt
     4261 acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga
     4321 tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct
     4381 gtggcgagat cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag
     4441 atggcgcgaa agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg
     4501 cgattgccgt tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt
     4561 ttttaacccc agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca
     4621 acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca
     4681 cacgtttgat tgagctggcg ctgaagcgtc acgctgcgga taacgcattg aaagccacaa
     4741 tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac
     4801 gacgaataat caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat
     4861 aggtctgcca accttttgct tcaaatccca gtaaccccac cacgccatta agaatgaata
     4921 taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca
     4981 tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg
     5041 tagacaacat tttcgtaatt gttaataaat ttaacttaag gttgccattt tgtaactcac
     5101 aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga
     5161 caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca
     5221 gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa
     5281 aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt
     5341 gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc
     5401 gtttaatgca ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca
     5461 ccattttcgg gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat
     5521 ggctgcactg gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc
     5581 cttatctggc atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc
     5641 tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg
     5701 aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata
     5761 aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg
     5821 gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt
     5881 tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc
     5941 gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt
     6001 aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta
     6061 agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat
     6121 tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc
     6181 accggtgcgc accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat
     6241 cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct
     6301 ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat
     6361 gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac
     6421 ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt
     6481 ttccagcgtc ggtgccagcc cctgactttc aaaggtgatg gcgagatctt tcgcccaggc
     6541 atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc
     6601 tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac
     6661 cgccaacaat gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct
     6721 caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag
     6781 acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa
     6841 agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt
     6901 accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata
     6961 gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg
     7021 tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc
     7081 ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg
     7141 cagtttgatc cctaccactg ccagccatcc ggtccccatc agcgaccaga cgatagcggc
     7201 aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag
     7261 cgttaccagc accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc
     7321 catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc
     7381 ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca
     7441 gcggaacaca tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag
     7501 cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg
     7561 cgaacttagc gcggtttgaa tcagatcata gaacggtgca taccaggcgt tgacggcgac
     7621 cccgacttcc accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca
     7681 gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac
     7741 aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg
     7801 gatctgcccg gaagcgccgg tgatacgcgc cacccagtct cccccaccgg cttgccagaa
     7861 gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgctcccg gctttgggaa
     7921 aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat
     7981 taaccgcatg accactacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca
     8041 aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag
     8101 attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg
     8161 aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct
     8221 ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca
     8281 ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc
     8341 gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat
     8401 gccgatcata ttttttacgg cagcggtgca acggggatgg cgctggttgt tatcgacggt
     8461 aaccagcgcg tctttcgtag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag
     8521 ctggattcgg tcgtgcgtat tgcttccctc accaaactaa tgaccagtga aatgctggtg
     8581 aaattgctcg accaggggac cgtaaagtta aacgatccgc taagtaaata tgctccgcca
     8641 ggcgcacgcg tgccgaccta caatgggacg cccattaccc tagtgaatct ggcaacccat
     8701 accagcgccc tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg
     8761 ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc
     8821 agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg
     8881 tccggcaagc cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa
     8941 gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt
     9001 gccagtccgt gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct
     9061 ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac
     9121 caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc
     9181 atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa
     9241 gagggtcgtc cggggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg
     9301 gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg
     9361 cgctttaaaa atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa
     9421 ccgttggtta tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag
     9481 tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac
     9541 gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg
     9601 caattcttca ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt
     9661 atgcccaatg agctgcacgt cgcgtaattc cagaattcta ttttgccaag ctaaccagta
     9721 aaaagcgtct cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag
     9781 atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa
     9841 accatcagcc agccccataa tataaggggc ctgggtaata ccgataagta cgttttcttc
     9901 tctacgcaga gagataactc cctctaaaat aacgaaagta tcttcgttat tgacatcact
     9961 ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca
    10021 tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt
    10081 tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac cagtatttct
    10141 tcggagtgaa gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc
    10201 tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct
    10261 gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc
    10321 accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg
    10381 agtcagagcc tgatcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata
    10441 acgcatgctg tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc
    10501 accagactgg aacagaccag aaacgctgcc gtaaggagtc acgtaaccag catcacccag
    10561 tttgaagtcg taaccggctt tcaagccgaa gccccaagcg tcggagttgg tgctaccgtc
    10621 aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa
    10681 gctaccatca acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg
    10741 gctgtcttga tctacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc
    10801 cgcaccgacg atccacttag cgttgttacc gtcaactttg gtatcaacac cgaccatgat
    10861 gccgttaaca tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa
    10921 gtagcttacc catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt
    10981 accaacggtg tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc
    11041 catgttagcg tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc
    11101 agcctgatag gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttggt
    11161 tttggtatca tcgatataga tgagctgctt acctgcgtaa tcagcaatcg caccagcgcc
    11221 agtggagttg tcgatacgaa ctttgtagtt accggttgca gctacaacgt tgtctgcaga
    11281 gctgttgtca acttcgtcgc cattgttaat gtcgttgtta ccattaacgg tcaagtgacc
    11341 atcagagttc atcgcaataa caccataacc gtagttagct ttgctggtgt cgttggtacg
    11401 atcgttgacg aggtctgcat tcaggatgta atcattacta tgaatgtcaa acacacctgc
    11461 gtaaacatta ccgttgttat ctttaacgtt gccactggtc agttccagag tatcagttgc
    11521 aaatacgctg gagatgtcat tcatgttggt gtttacatcc agcacaccac cgttagtaac
    11581 ggtaatggta ttggcataca gcggtgcata tgcccaagct gaagcagtat cttcaccgaa
    11641 gttaacggtt gagaaggtat caatggtcag gtgatcggtg ttaacttcac cgttaacttc
    11701 cattgaagaa ccaccgttca gagtgatggt gtcagaagtc agagaagaat ctgcactcac
    11761 gttaacctga gaaccgctgt tgatcgccag ggtatcgatc agggaagttt tcgtggtttc
    11821 ccaatcagaa ccgttgttca gaacaacatc gaatacgccg ctctggaatg cgttagcgtt
    11881 atcaacacga ttccagccat cttcagagta agaagcgtac gggttcaggc tgtttggtgc
    11941 aacgtcgtag aaggcttcgt cagcaacaac aacgtcataa tcaatattcg ctgaaccaac
    12001 ccatttggtg ttgttcagag tcaggttcag gtgatcgacg ttctgatcgt cagttgccca
    12061 accgccatga gtgtaagcag tggtagagtt gtcaatcagg tgacccgtcg cgtcaaagtt
    12121 agcgttccag gtactgtcaa atacaacgtc accaagcaac tgagagttgt tgaacgtaac
    12181 gttgttgacc atacgataat cagaagtgtc atcatcgatg aaggctaacg caacatcatc
    12241 agcaggagat gcgccattgc cgtagtcact tggttcaaca gaattgccga aatgaccacg
    12301 atctgattgt tcgtcttcag tccatgaacc agaagttaca gtagagtcat taacaacgat
    12361 gttgttgtcc cactgatgac cttcggtatt gtaggtcgca ttaccgaggt cgtgttgaac
    12421 caaagtaata cctgcaacat gggagttgtt gctgatgttg atgttacttt caacatccag
    12481 agtcacagcg gtaccaagac cagcggttac caaacgatca taatctgtct tagatgtttt
    12541 accgtctgca tccagataag tatcggtgaa gtagaaggcg tcaaaatcat cgtcaatagt
    12601 agaattagcg atgttcagag tgaagatgtc gccatcaacc cagtcgtggc cagtataatc
    12661 gctaccgttg gtccagacgt agtcgccata accaacctgg taagatgtaa ccataccatg
    12721 aacaattgag ttggagatgt tcagtgtatt accggtatct gtgccataat cgtggttcag
    12781 ataataagta gaaattacac catttacagt gtcgccgctg aaagtggtat caaagtaacc
    12841 atcagctgcg tggtcatata cataaccacc gtaagtcaga taatcgtgat caattttagt
    12901 gctgtcaccc caattgtaga tggtggctgc gtcatcgtga tacccgaagg tgttataagt
    12961 agctgcatta gcctgagagg tgatagccag agtgcaagcc aatgctaatt gtgatactac
    13021 aagtttcttt ttccaggagt gcatttgtca tccctcctca gggacgtaat aagttgatcc
    13081 atcaattatc aatgcataga aaacatatta tgtttcgcgc taacgattat gctgcgttac
    13141 agacgaatgg ttcaattatt ctttccccta agtccgaatg tggacatata taatttatgg
    13201 agaaaaacac gcatcaaatt gataaaagga cgatatttca acatgttgca gataatgtta
    13261 tttttgatac atacgcgtta taaatatcaa aaacacaaac cagataaata tcttatcaat
    13321 atacaacaaa gtttgaatta ttcaatttat agcagcacaa ccaaacgcca aaaaaaataa
    13381 taaccaagag ctataaataa agattaaaaa tattttcagt gaatatgaaa ttattgctat
    13441 ttctattaca aagatgtcct ggtctctctt tcattctctt cttgtcaaca tcacgacaac
    13501 tttcgtaaaa cactcctgcc ccgcttcagg tatactgtgc accttaattc cacaaacatc
    13561 aggcagacca tgacagactt aatccaacgc cctcgtcgcc tacgcaaatc tcctgcgctg
    13621 cgcgctatgt ttgaagagac aacacttagc cttaacgacc tggtgttgcc gatctttgtt
    13681 gaagaagaaa ttgacgacta caaagccgtt gaagccatgc caggcgtgat gcgcattcca
    13741 gagaaacatc tggcacgcga aattgaacgc atcgccaacg ccggtatccg ttccgtaatg
    13801 acttttggca tctctcacca taccgatgaa accggcagcg acgcctggcg ggaagatgga
    13861 ctggtggcgc gtatgtcgcg catctgcaag cagactgtgc cagaaatgat cgtcatgtca
    13921 gacacttgtt tctgtgaata cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc
    13981 gtcgacaacg acgcgactct ggaaaattta ggcaagcaag ccgtggttgc agctgctgca
    14041 ggcgcagact tcatcgcccc ttccgccgcg atggacggcc aggtacaggc gattcgtcag
    14101 gcgctggacg ctgcaggctt taaagatacg gcgattatgt cgtattcgac caagttcgcc
    14161 tcttcctttt atggcccgtt ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa
    14221 agctatcaga tgaacccaat gaaccgtcgc gaggcgattc gtgaatcact gctggatgaa
    14281 gcccagggcg cagactgcct gatggttaaa cctgctggag cgtacctcga catcgtgcgt
    14341 gagctgcgtg aacgtactga attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg
    14401 atgattaagt tcgccgcgct ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc
    14461 ttaggttcaa ttaagcgtgc gggtgcggat ctgattttca gctactttgc gctggatttg
    14521 gctgagaaga agattctgcg ttaatttttt tcctctcaga aagggggagt taacctgttg
    14581 aaagtacggt gaacgccccc tcaccctaac cctctcccca agggggcgag ggaaccgtcc
    14641 gcactcgtat caccccgccc gataaaacgg tttatcccca aggatcgtcg cccgatgcat
    14701 aatccgccgc tgtggcaggt aatcggcatt ggcatagtgc tgagtcacgc ggttatccca
    14761 aatcgcaata tcattcggtt gccagcgcca acgcacctga aactccggtt tagtgatatg
    14821 ggcgaataaa aagcctaaca aggcttcgct ctctttctcg ctgacatcga caattcgcgt
    14881 agtaaagcct tcattcacaa acagcgcctg tttaccgctc accggatgcg ttcgcaccac
    14941 cggatgtagc aacggaggat ttttcgcgac cgcctcgcgc cagcgttgat gttcctcctc
    15001 ggttttgcgg tatttgtatt ccgggaacga tttacggaaa tcatgctccg cacgcagccc
    15061 gctcagcagc tggcggaagg gaacagagag cgcctcatag gccgcaatac cgctggtcca
    15121 gagcgtatca ccgccggtcg aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg
    15181 cgtttcaata aatgtcacat cggtatgcca gttgtcgtta tccggcggat tatcgttatg
    15241 ggtatccagc acgatgatct cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg
    15301 cagttcgcca aaacgctggg ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg
    15361 cagaaatacc acctgatagc gcagcaccgc atggtaaagc tgttcaaact gattatcgct
    15421 taacgggcgc gtcaggtcgg cacccgaaat ttgtgcgccg atatacggcc ccagcggggt
    15481 aatactcaga cgttcactca ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac
    15541 gcgcgcagac ccagttctaa aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagt
    15601 accacgtcag tcgcgagaaa ttcacccgct gactgaacca taaatcctaa accgcgtgtc
    15661 gcggcaatca gctccgccgc taccagcgta gaccagccca cacccagacc aatgcgtaat
    15721 ccggtgagga tttccggcag cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg
    15781 ctggcaccca gcgactgggc ggcacgaatg cgaacctgct gcgcgctttt cacccccgcc
    15841 agcaccgaca tcgccaccgg tgcaaaaatc gctaaataga tcaataagat cttcgaggtt
    15901 tcaccaatac caaaccagat caccatcagc ggcaaataag ccagcggcgg caccggacga
    15961 taaagttcga ttaccggatc cagaatgccg cgcaccgtcg ggcttagccc catggcgatc
    16021 ccgaccggaa taccgatcac caccgccgcg agtagcgcca gcacaatgcg cgtcaggctg
    16081 gctgccagat gctgccacag cgtggcgtcc ataaatccct gcggtccggc aatggtgagc
    16141 agttttgcca gtacttgttg cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc
    16201 gccaccgtcc accatacagt gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga
    16261 cgcgagagcg gccagcgcca tttcagccgc cgcgaatgca gtttttcatt aatgagcaca
    16321 ctcatgagaa cgcctcccgt tgctcaaata cgcggcttaa aacatattcg cgcatggcga
    16381 tgaattgtgg atcggacttg atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa
    16441 agttgagcgg cagccgctcc agcacacggc cagggccgga tgaaagcaga accagttcag
    16501 tcgccataaa caccgcttct tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct
    16561 cctgccagag tttcagcagc agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac
    16621 caaacggttc gtcgagtaat aacagctggg gattcgccgc cagcgcgcgg gcaatcccca
    16681 cccgctgacg ttgaccaccg gaaagctgcc agatatagcg tttttctgcg ccttccagcc
    16741 ccactttttt cagcatctgg tgcgcgattt ccagtcgctg cattttctct atacctgcca
    16801 attgcaggcc gaacgccacg ttgtcctgta cattgcgcca cggtagtagc ccttcattct
    16861 gaaaaaccac cccgcgctct gctcccggtc cctcaatacg cttacccgcc agttgaatgc
    16921 tgccatgctg ataaggcaca aaaccggcaa tcagattcag cagggtggtt ttaccgcagc
    16981 cggacggccc cagcaccacc agtagctcgc cgctttccag cgtcaggttg atatcttcca
    17041 gtgccggttt gccgccataa tcggcataga gatgagagat ttgcagcatc tgcgcctcct
    17101 tttattgcac gaagcgcgag gtaacgtact ggctgtaatc attcgctaca gccgggacct
    17161 tgccctgctc tttcaaaaac tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca
    17221 gttctgccgt ttgttgctgc ggcgtcagat aggtattccc cttcaccagc cccggaacgt
    17281 caccttccgg cacgccgctt aaacgcgcca gtttgctgat gttttccggc tgtttcagcc
    17341 acgcgtctgg gttagcaatg tacggttgct gagcatcgat ggcgctttta gcgaacgctt
    17401 tcacgacctc aggatgtttc tcggcaaaat ctttgcgcac cacccagacg tccagcgttg
    17461 gcgcacccca ctgcccgacc tgttcagaat cggtcaacac cttgccgtct ttttccaggg
    17521 cgttaaccgc cggtgcccag acataagcac catcaatatc accccgctgc catgcggcga
    17581 taatcgcggg cggctgcagg ttcacaatct ccacttgccc tggtttaatc ccccagtgtt
    17641 tcagcgccgc cagcaggctg tagtgggtgg tggagataaa cggtacggca atgcgtttgc
    17701 caatcaaatc ttcgggtttg ctgatagttt tctttaccac cagcgcttcg gagttaccca
    17761 gttttgacgc cagcaagaag acttcaatcg gcacctgttg gctggctgca accgctaatg
    17821 ggctggaacc gaggttgccg atttgcacgt cgccagaagc cagcgcccgc acgatgctgg
    17881 ctccgctgtc aaacttacgc cagtccacgc tcgccccgct ctctttagca aaggtgttgt
    17941 cggcctgagc cactttcgcc ggttcggctg aggtttgata cgccacggtg acgttcaccg
    18001 cctgtgcctg aaaagcgatg aatgccagtg cggcaagaag tgtgttacgc gatgaaattg
    18061 ccatgattgt ctgctcccct gtcttgttat gggagcagta ttcaggaata aaaacattca
    18121 ttaaaagaat tagtcgttat cgcacagatg attttattct tagcaaaaaa acggcaaagg
    18181 aaagatt
//
LOCUS       DAJTVJ010000086        17767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000086 DAJTVJ010000000
VERSION     DAJTVJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.086"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(47..1021)
                     /gene="cysB"
                     /locus_tag="OO448_002844"
     CDS             complement(47..1021)
                     /gene="cysB"
                     /locus_tag="OO448_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6338012.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1231..3828)
                     /gene="topA"
                     /locus_tag="OO448_002845"
     CDS             complement(1231..3828)
                     /gene="topA"
                     /locus_tag="OO448_002845"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6338013.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4208..4459
                     /locus_tag="OO448_002846"
     CDS             4208..4459
                     /locus_tag="OO448_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6338014.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4495..5544)
                     /gene="sohB"
                     /locus_tag="OO448_002847"
     CDS             complement(4495..5544)
                     /gene="sohB"
                     /locus_tag="OO448_002847"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6338015.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5764..6522
                     /locus_tag="OO448_002848"
     CDS             5764..6522
                     /locus_tag="OO448_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6338016.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6519..7109
                     /gene="cobO"
                     /locus_tag="OO448_002849"
     CDS             6519..7109
                     /gene="cobO"
                     /locus_tag="OO448_002849"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6338017.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7149..8021)
                     /gene="rluB"
                     /locus_tag="OO448_002850"
     CDS             complement(7149..8021)
                     /gene="rluB"
                     /locus_tag="OO448_002850"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6338018.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8234..9348)
                     /locus_tag="OO448_002851"
                     /pseudo
     CDS             complement(8234..9348)
                     /locus_tag="OO448_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9376..9996)
                     /locus_tag="OO448_002852"
     CDS             complement(9376..9996)
                     /locus_tag="OO448_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6338019.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9993..10874)
                     /gene="yciV"
                     /locus_tag="OO448_002853"
     CDS             complement(9993..10874)
                     /gene="yciV"
                     /locus_tag="OO448_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6338020.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11012..11056
                     /gene="trpL"
                     /locus_tag="OO448_002854"
     CDS             11012..11056
                     /gene="trpL"
                     /locus_tag="OO448_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6338021.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11033..11126
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11148..12710
                     /gene="trpE"
                     /locus_tag="OO448_002855"
     CDS             11148..12710
                     /gene="trpE"
                     /locus_tag="OO448_002855"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6338022.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12710..14305
                     /gene="trpD"
                     /locus_tag="OO448_002856"
     CDS             12710..14305
                     /gene="trpD"
                     /locus_tag="OO448_002856"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6338023.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14306..15667
                     /gene="trpCF"
                     /locus_tag="OO448_002857"
     CDS             14306..15667
                     /gene="trpCF"
                     /locus_tag="OO448_002857"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6338024.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15679..16872
                     /gene="trpB"
                     /locus_tag="OO448_002858"
     CDS             15679..16872
                     /gene="trpB"
                     /locus_tag="OO448_002858"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6338025.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16872..17678
                     /gene="trpA"
                     /locus_tag="OO448_002859"
     CDS             16872..17678
                     /gene="trpA"
                     /locus_tag="OO448_002859"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6338026.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4246 a         4721 c         4451 g         4349 t
ORIGIN      
        1 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
       61 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      121 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      181 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      241 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      301 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      361 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      421 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      481 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      541 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagtgaaa tcagcattgc
      601 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      661 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      721 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      781 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
      841 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
      901 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
      961 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1021 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1081 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1141 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1201 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1261 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1321 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1381 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1441 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1501 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1561 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1621 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1681 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1741 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1801 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     1861 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     1921 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     1981 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2041 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2101 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2161 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2221 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2281 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2341 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2401 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2461 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2521 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2581 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2641 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2701 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2761 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     2821 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     2881 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     2941 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3001 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3061 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3121 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3181 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3241 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3301 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3361 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3421 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3481 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3541 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3601 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3661 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3721 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3781 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     3841 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     3901 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     3961 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4021 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4081 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4141 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4201 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4261 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4321 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4381 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4441 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4501 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4561 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4621 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4681 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4741 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4801 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     4861 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     4921 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     4981 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5041 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5101 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5161 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5221 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5281 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5341 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5401 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5461 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5521 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5581 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5641 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5701 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5761 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     5821 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     5881 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     5941 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6001 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6061 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6121 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6181 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttgggcg tcagggacga
     6241 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6301 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6361 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6421 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6481 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6541 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6601 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6661 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6721 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6781 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     6841 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     6901 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     6961 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7021 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7081 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7141 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7201 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7261 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7321 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7381 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7441 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7501 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7561 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7621 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7681 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7741 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7801 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     7861 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     7921 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     7981 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8041 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8101 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8161 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8221 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8281 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8341 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8401 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8461 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8521 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8581 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8641 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8701 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8761 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     8821 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     8881 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     8941 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9001 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9061 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccaccag gctgcatcca
     9121 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9181 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9241 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9301 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9361 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9421 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9481 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9541 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9601 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9661 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9721 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9781 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
     9841 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
     9901 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
     9961 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10021 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10081 agttcaatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10141 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10201 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10261 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10321 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10381 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10441 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10501 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10561 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10621 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10681 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10741 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10801 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    10861 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    10921 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    10981 tgcgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11041 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11101 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11161 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11221 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11281 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11341 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11401 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11461 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11521 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11581 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11641 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11701 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11761 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    11821 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    11881 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    11941 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12001 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12061 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12121 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12181 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12241 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12301 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12361 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12421 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12481 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12541 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12601 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12661 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12721 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12781 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    12841 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    12901 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    12961 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13021 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13081 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13141 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13201 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13261 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13321 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13381 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13441 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13501 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13561 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gctgcctgtg ggctgaaagt
    13621 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13681 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13741 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13801 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    13861 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    13921 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    13981 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14041 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14101 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14161 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14221 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14281 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14341 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14401 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14461 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14521 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14581 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14641 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14701 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14761 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    14821 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    14881 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    14941 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15001 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15061 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15121 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15181 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15241 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15301 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15361 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15421 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15481 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15541 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15601 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15661 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15721 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15781 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    15841 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    15901 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    15961 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16021 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16081 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16141 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16201 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16261 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16321 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16381 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16441 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16501 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16561 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16621 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16681 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16741 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16801 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    16861 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    16921 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    16981 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17041 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17101 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17161 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17221 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17281 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17341 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17401 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17461 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17521 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17581 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17641 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc cccctgccgc cagttccgct
    17701 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17761 cttttta
//
LOCUS       DAJTVJ010000087        17733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000087 DAJTVJ010000000
VERSION     DAJTVJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.087"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..183)
                     /locus_tag="OO448_002860"
     CDS             complement(<1..183)
                     /locus_tag="OO448_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6338027.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLT"
     gene            complement(393..911)
                     /locus_tag="OO448_002861"
     CDS             complement(393..911)
                     /locus_tag="OO448_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6338028.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1608..2108
                     /gene="tssB"
                     /locus_tag="OO448_002862"
     CDS             1608..2108
                     /gene="tssB"
                     /locus_tag="OO448_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCR6338029.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2143..2367
                     /locus_tag="OO448_002863"
     CDS             2143..2367
                     /locus_tag="OO448_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338030.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2418..3893
                     /gene="tssC"
                     /locus_tag="OO448_002864"
                     /pseudo
     CDS             2418..3893
                     /gene="tssC"
                     /locus_tag="OO448_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            3900..4313
                     /gene="tssE"
                     /locus_tag="OO448_002865"
     CDS             3900..4313
                     /gene="tssE"
                     /locus_tag="OO448_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6338031.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4317..6167
                     /gene="tssF"
                     /locus_tag="OO448_002866"
     CDS             4317..6167
                     /gene="tssF"
                     /locus_tag="OO448_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6338032.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMQPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6131..7213
                     /gene="tssG"
                     /locus_tag="OO448_002867"
     CDS             6131..7213
                     /gene="tssG"
                     /locus_tag="OO448_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCR6338033.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7238..8518
                     /gene="tagH"
                     /locus_tag="OO448_002868"
     CDS             7238..8518
                     /gene="tagH"
                     /locus_tag="OO448_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCR6338034.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8515..9039
                     /gene="tssJ"
                     /locus_tag="OO448_002869"
     CDS             8515..9039
                     /gene="tssJ"
                     /locus_tag="OO448_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCR6338035.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9042..10371
                     /gene="tssK"
                     /locus_tag="OO448_002870"
                     /pseudo
     CDS             9042..10371
                     /gene="tssK"
                     /locus_tag="OO448_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10376..11137
                     /gene="icmH"
                     /locus_tag="OO448_002871"
     CDS             10376..11137
                     /gene="icmH"
                     /locus_tag="OO448_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCR6338036.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11146..13929
                     /gene="tssH"
                     /locus_tag="OO448_002872"
                     /pseudo
     CDS             11146..13929
                     /gene="tssH"
                     /locus_tag="OO448_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            13926..14669
                     /gene="tagO"
                     /locus_tag="OO448_002873"
     CDS             13926..14669
                     /gene="tagO"
                     /locus_tag="OO448_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCR6338037.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14674..16086
                     /gene="tssA"
                     /locus_tag="OO448_002874"
     CDS             14674..16086
                     /gene="tssA"
                     /locus_tag="OO448_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCR6338038.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16105..>17733
                     /gene="tssM"
                     /locus_tag="OO448_002875"
     CDS             16105..>17733
                     /gene="tssM"
                     /locus_tag="OO448_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCR6338039.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPYV"
BASE COUNT         4133 a         4853 c         4854 g         3893 t
ORIGIN      
        1 cgtcaggtag gccattttgt ccagtacctg cggaaattca agggagagaa actgctggct
       61 gaccagagag agctcgaggg aaaagagcga agagagtgac tggttcagat gaaaggagac
      121 caccgcaaaa gcatccggtg gcaggccgtc cacttccagc gtgaaacgta atccggttga
      181 catagcaacc tccggggtta aggaaaaaca gacagttcgc tgttaaagcg gctgtgacag
      241 tacagtgtta cgcttgcgtt caggcacgct accgccgaag tttccggcat ccgccatctc
      301 ccgtgaacgt aaccccttac tgacaacctt tctttctacg gcgtaataca aaaacagcat
      361 ccggcctgat agccggatgc tgttggctta acttatgctt ccagtggtgc gcgccagtcg
      421 tcggcaccgg aggtgccaga tttgacgtgt tcccattcaa ttttgcggta ggccagtgaa
      481 acctcgagca actgggtgaa ctcgcgtttc gccgggtcct ggcaatgcgg catatgcagt
      541 ttcatatcga caatcgtcga atcagtcagg cgggtagtga aataatgctc ctgtttgccc
      601 tccacagagg tacgccacca gtgaagttca gtggttggca gcatttcgcc ggaggccagt
      661 gcgttgtaca tcagcggaac cgctttgttc agcgccacgg taaaaatgaa aggcttgtga
      721 gcacgctgac ccgcaggctg accggactga ggagtggtcg ggacagtaac gttatgcagg
      781 aattcctgca ccagcatctc gtcttcgtgc ccctgcacat agatgttgcc gacagaatca
      841 gcggtaaatg cacctgcagt aatatttccc tgggtctgac cagtgatgga gatataacac
      901 ggtgttggca tttgtaaact ccttgttaac gtggttaatt actacttaca atgacctgcc
      961 gttaatccca gacaggcaga tgcagctcac ttcacggaac cgcatcattt ttcagtattt
     1021 tttacataca aaccgagcta gaatttacgg ttgtcgtaaa aactattttt aaaatcatta
     1081 aatctcttat tcagcaataa cttaataata tatatctgga taaccatacc caactgttca
     1141 aagtccgtat tgcctccata aaatggccaa agctgaatca tgaataaata ttgtatttat
     1201 cctttctgaa ttatcatttt gataattttt gagatattcc ttaaaaatac ccacttaatc
     1261 aatcacaaaa caaatatata aacaagcaat cattagatta gcttattttt aatgaaatta
     1321 ttatatactt caataagtta cacaaaaact ctatatttaa attattgcca taatacacct
     1381 gaagcgaatt gcacaggttc acacaatcta tttaacgctt gcagaatacc cctctttacg
     1441 atcgataaaa agtccttttc ttatttattt tgaagcgtaa aatgcggggt gatgaaggag
     1501 tggtgatttt attttcaccg ccattagggc ggcattgccg atattttttt gcggtacaaa
     1561 tatgccgtgt tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat
     1621 ttgaaggcag cgtcgcgccc cgcgagcgta ttaatatatc gtatgtccca aaaacagatg
     1681 gccagacggc tgaagtggaa cttccgctta acatgctcgt tgtcggcgat accggaaata
     1741 cgcaggaaac atccccactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg
     1801 gtgcagtgat ggctgaagca gccattggcc tcaattttac tgtccccgcc acgctgaagg
     1861 gaagcacaac agacgatgaa ctgaatgtgg cgctgaatat caaatcactg gatgattttt
     1921 caccagatag cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag
     1981 cactgactgc actgaagggg ccgatgggta accttccggc attccgtacc cagctccagg
     2041 cattgctgga aaatgaagag tcccgcgagc agttgctcaa agaaatcggt ctggtgagca
     2101 ataaataacc accactattt cattaaacag ggttatccag gcatgacggc acacaaaaat
     2161 atctccggga cataccacct ctctgttgca gacattctgc aggtggttta ccaggtatgt
     2221 ttcagcccgt ccgttgaaat caaccaggat ggtgtggctg cgctgattac aacactggat
     2281 cgccgtatca gtgatctatt ggatgagatt atccattttt gtgagtttca gcagagcgcg
     2341 tcgcactggc agcgcgtact tcactgatgg cgaaaagccg gtttcagaaa cacagctaaa
     2401 acagaaggat tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc
     2461 agcctgagca ggcatcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcccg
     2521 gttcggaagg ttatgacgtt gccctccagg gtgtgacagc ctttattgcc agcatcctgc
     2581 aatccactgc atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata
     2641 ttgatgaacg catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg
     2701 tcgagtcgtt ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca
     2761 tcaaggtcaa cgtgcttcat gtcaccaagc aggaacttct ggaagacttt gagtttgcgc
     2821 cagaaattat tcagtccgga ttttataagc acgtttattc gtccggtttc ggacagtttg
     2881 gcggtaaacc catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca
     2941 tgaaactgct gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct
     3001 ccgtttcccc ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc
     3061 tttatgccat ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag
     3121 attcgcgcta tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa
     3181 cagataaccc tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt
     3241 atctgtgggg taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat
     3301 accgctgatg tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccga
     3361 tgcatctgtt cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca
     3421 ccgatcgccg cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag
     3481 actctgataa tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg
     3541 gaaaagatgc agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca
     3601 atcggttagc gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa
     3661 ggagcgactt agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa
     3721 acccgcctgc tgacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg
     3781 atgtagaagg cgaacccggc tggtaccagg ttgcgctaag cgtgagacct cattttaaat
     3841 ttatgggggc aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca
     3901 tgataccgga tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcgg
     3961 ccagaaaccg gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac
     4021 gaaacattct taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg
     4081 aaccggaagg ggctgaaaac ttccgcgaat ccatgagtcg ggccatcgag caatgtatag
     4141 aacgctatga accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca
     4201 gcccgctgga tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg
     4261 tgcttgaatt tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg
     4321 aatttgaaga acgctatttc cgggaagaac tcgattacct gcgccagctt agcaagctgc
     4381 tggcaacgga aaaaccccat ctggcccgct tcctggccga aaaagatgca gatccggata
     4441 ttgaacgcct gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg
     4501 atgaattccc agaactgacg cacgggctta ttaagatgct atggcctaat tacctgcgtc
     4561 cggttccggc aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg
     4621 tgttaatccc ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag
     4681 tgctgccctc tgatgctaaa aaggaggagc cgcctccctg taccttcacc ctctgccggg
     4741 atatctggct gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg
     4801 gtgttatcaa catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga
     4861 acaaacttcg cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat
     4921 ggttctgcga atacttgcag ggtgccgacc tgactgtggg tgaacagcat attcgcctgc
     4981 ctgagtttat gctaaaagct gtcggttttg agccgcagga tgccatgcag ccctggccga
     5041 aaaacgtcca cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc
     5101 tcttttttga tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta
     5161 ccctgcaact gcgtttttcg cgccctttgc ccgtggacat ccggctgcgc cgcgattccc
     5221 tgcgcctgta ttgcgcacct gccattaatt tatttatcca ccatgcagaa gccatcacgc
     5281 tggacaaccg gcgggcagac tatccgctgg ttcccagccg ccattaccca caacattacg
     5341 atgtattttc cgttaacagt gtggtgagcc aggtccagga tatgttcagg aaaaaagatc
     5401 tggggcgtcc tgtttcgacg caggccgcgc gccagtggcc agcctttgaa agtttcagcc
     5461 atcagataga atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat
     5521 ccctgttcca tcgcggcttt gatcataccc ttgcctttat acatgctgat ggcagttatc
     5581 cgtcagacga atctctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc
     5641 gtgagcttcc gtcacaaatt cgttccggcg atatcaccgg cacaaccgga aaaaatgcgg
     5701 ctgttgcttc atttcgcaac attacccgcc cgacgcaacc actctggccg gtcattgatg
     5761 gcagcctgca ctggccccta ctctccgcca tgaacctgaa ttatctgtca ttactggata
     5821 cggacgcgct gaagcaggtc atcgccaact ttgatcgcca cgcaatccat catccgcaga
     5881 cggcacggct gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccccgttg
     5941 atcgcctgtt tacgggtatt cccgtccggg ggctggcttc cacgctgtat ctgcacccgg
     6001 agccgtttgt ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt
     6061 cgctgtacgc cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg
     6121 agacatggaa atggacggaa agaatcgggc agcatcctct tatctgagtc cgggaaatcc
     6181 cccggcagat aaagagcaga atgatccgct ggcacaggtt ttccataatg cctgctctta
     6241 caattttttt gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc
     6301 agaattatcc ctgcgcgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag
     6361 ccttgccttc ccgtgcagtg atatcagtgc gctgaaacgg gatacgtcag gggcattcag
     6421 gatgaccacc acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct
     6481 cgctcacctg gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat
     6541 gttcagccac cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat
     6601 cagttttcgt aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct
     6661 gggccaccgc cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc
     6721 cgggatcctg gcaaatccgg gacgatcccc ggaaattatc tgtggcctgg tgtcccactg
     6781 ctttgatttg tcagaagtga cactgcaaaa ctggcaacgt cgcaaggtgg atattgaacc
     6841 ggatcagcaa aatagtctgg ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag
     6901 atcggtactg ggcaactttg ttctgggtac acgagtgccc gatctcagcg gaaaattcca
     6961 gctcagtatt accagcctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa
     7021 cttcctgcca ctgacgatgt tcgtgtcctt catcctgcgc gatcagcttg cctgggactt
     7081 acatctgggg ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc
     7141 gctgctgggc tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag
     7201 ggtacggtcg taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac
     7261 gctttcattg caggtcatta acggcagtga gctggaaagc gggcgggcgg cgcgctgtct
     7321 gttcacacag cagggaaatg tcggccatgg ccccgaatgc cactggtcgg tacaggatcg
     7381 tcagcagagc attccggccc aggcttttac cgttatcctg cacgatggca cattttgtct
     7441 gcgcccgcag acggcacaac tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct
     7501 aatacagttg cgccagggcg atgagatcca gatcggacgg ctgatggtga gggttcatct
     7561 gaaccgggga gatattcccc attacgatga ggaaatggcc actcccgaaa ccatcgttac
     7621 caatcgcgat atgctcacgg ataccctgct atcaacggag ggtgcgccac actatccggg
     7681 aatgactcac cggcaccagc ttgcagacac cgtggtaaac ggtttttctg ccgatccact
     7741 ccaggctctt cagtccgaaa gcctgattac cacgggcgat ccgctttcag gcattgcggc
     7801 tgtccggcca tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt
     7861 tatggatctg ccgcccattt atgccagccc cggcgatcgt aatgacgacg tctctgcggc
     7921 agaaatggca caacgccacg ttgcggtcac ccccttactg cgcggtcttg gcggctcgct
     7981 taccatgagc aattccgacg atgcggatga ttttctggag gaggccggac gaacgttaca
     8041 ggccgcaata aaaggtctgc tcgatttgca gcagcagcgt aacagcctct cagacaaaca
     8101 tttgcgcccg ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga
     8161 cgtaatgttt gccgaaggta aaagcccggt acatctggcg gctcccgccg ccgtcagtga
     8221 aagcctgcgc aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcgct
     8281 tcgtgtcctg ctggatgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg
     8341 ccgcagccat gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca
     8401 ttattacgaa gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt
     8461 ctacgcccag gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac
     8521 aggaaggcgt ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg
     8581 aaaaaaatca gtgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa
     8641 ccttcggaaa taaccgtcac cctgctgacc gaaccggaca ccaataccaa tgcggaaggt
     8701 gaatcagccg ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgctggca
     8761 gccgactatg accagatagc cagcacgccg ctaccggatg tgctgggtaa aaactatatc
     8821 gaccaccagg atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg
     8881 gatgagaaaa cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa
     8941 tggaaacaaa tcgagacggt ggaaggaatc ggccaccatt accgcctgct ggtgcatgta
     9001 cgtcaaagca gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca
     9061 aggtgatgtg gcaggaaggg atgctgatgc gtccacacca tttccagcag cagcagcgtt
     9121 acaacgacta cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta
     9181 ccgaactcac cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt
     9241 caggcacact gcccgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc
     9301 cgctgcaccc gcaaaatttt ccggacgaaa gaagccgcaa tatctacctc gctctgccgg
     9361 tcgccagtga tgtgagaaat gaaatcagcg acggacggcg aatcgggcgt taccggctga
     9421 attatgccga tgtccgggat ttgcattcag aagaaggtga cgcgcgaaca ttgacgctgg
     9481 gtcaactgac gccgcgcatt atgagcggtg cagaagatat gagcgcctat attacgctcc
     9541 cgctttgccg tatcagcgat cgccgccgat ggctccctga cgttggatga cgattttatc
     9601 ccctcctgcc agaatattca ggtcagtaag aaactgcgtg tttatctcaa agaggtacag
     9661 ggggccattg gcggacgggc aagcgatctg gcaaaccgta ttggctctcc ggcgcagagc
     9721 ggcatcgcgg atgtggcgga atttatgatg ttgcagttac ttaaccgtaa ccagacccgg
     9781 tttacccatc acgcccgtcg atcccagctc cacccggaag atttctacct tgatcttgcc
     9841 gggttgctgg gtgaactgat gacctttacc gagccgtcgc gcctgccctg cccgcttgat
     9901 gtgtatgatc atcatgacct gaccaaaata tttaaaacgc tgttaccgga agtcaaacgg
     9961 gcgctgcata ccgtactgtc gccaagagcc gtcaatctgc cgctgcatct gcgtgacggt
    10021 atctggcagg ccgatatcca tgacacagaa ctgctgcaat ctgccacctt tgtgctggcc
    10081 gtggcggcaa acatgccggt cgatcagatc cagcgccagt ttatccagca gtcgaaaatt
    10141 tcctcgccgg aaaaaatccg caatatggtc agtgtgcaga tccccggtat tccattgcgt
    10201 gcccttatgg tggctccccg ccagcttcct taccattccg ggttcagcta tttcgaactc
    10261 gacaagagtg gccaggcctg gacagaaatg gctgccgccg gggccgttgc actgcatgtt
    10321 tccggcagtt tcccggatct gaacatgcaa ctgtgggcga taagagggta agacgatgga
    10381 tgaggggtca ctgtctctgc caccatttac cggacacgac gaaaaaagcc agcgcaacta
    10441 ccatctcgcg cttcgtggca acagcctgaa tccgatgatc gacgcggcca cgccgctact
    10501 cggtatggta atgcgccttt caaccatgaa cagccagacg atgccggagc atctttttgc
    10561 tcaggtggtg acagatgttc aggccgtgga gcaactgctt caggagcagg gctacgaacc
    10621 gggcgtgatt atctcgtttc gctacatcct ctgcacgttt attgatgaag cggcgctcgg
    10681 taacggctgg tcaaacaaga acgagtggat caagcagtca ttgctggtcc atttccacaa
    10741 cgaagcctgg ggtggcgaga aagttttcat cctgctggag cgcctgatcc gtgaacccaa
    10801 acgttaccag gatctgctgg aatttctctg gatctgtttt tctctgggat ttcgcgggcg
    10861 ctacaaagtg gcagcacagg atcaggggga atttgagcag atctaccgcc gtctgtacca
    10921 cgttctgcat aaactgcgcg gcgatgcccc tttcccgctg ttacaccagg acaaaaaaac
    10981 ccagggcggg cgttatcagc tcatcagtcg cctgactgtt aaacatattt tttgtggtgg
    11041 cgttgtcgtg ctggcgttgt tttacctgtt ctatctgttg cggctggaca gccagacgca
    11101 ggacatcctg caccagctaa acaaattact tcgataagga cgtttatgat ccagattgat
    11161 cttcccacgc tggtaaaacg gctgaacctg ttctcccgcc aggcgctgga gatggccgcc
    11221 tctgaatgta tgagtcagca ggcagcggaa attaccgtca gccatgtgct cattcagatg
    11281 ctcgccatgc cacgcagtga cctgcgggtt attacccgcc agggcgatat tggcatggaa
    11341 gagttgcgcc aggcgctgac ggtagagaac tacacaaccg cccgttctgc ggacagctac
    11401 ccggcgtttt ccccgatgct ggttgagtgg cttaaagagg gctagctgct ggcgtcggct
    11461 gagatgcagc acagcgaact gcgcggcggc gtgttgctgc tggccctgct gcattcgccg
    11521 ctgcgttata taccgcctgc tgccgcccgg ctgttgaccg gcattaaccg tgaccgtctg
    11581 caacaggact ttgtgcagtg gacacaggag tcggcggaat cagtcgtgcc ggatgcagac
    11641 ggtaaaggcg caggcacaat gacggacgcc tctgacaccc tgcttgcccg ctatgccaaa
    11701 aacatgaccg cagacgcccg taacggcagg cttgacccgg tactgtgccg cgaccacgaa
    11761 atcgacctga tgatcgacat tctctgccgc cgccgtaaaa acaacccggt ggtggtgggc
    11821 gaagcgggcg tgggcaaaag cgcactgatt gaagggctgg cgctgcgcat cgtggcaggc
    11881 caggtgccgg acaagctgaa aaacaccgat atcatgaccc ttgacttggg cgcattgcag
    11941 gccggggcgt cggtgaaggg tgaattcgaa aaacgtttca aagggctgat ggcggaggtc
    12001 atttcctccc cggtgccggt cattctgttt atcgacgaag cacataccct gattggcgcg
    12061 ggcaaccagc agggcgggct ggatatctcc aacctgctca aaccggcgct ggcgcgcggc
    12121 gagctgaaaa ccatcgccgc caccacctgg agcgagtaca aaaaatactt cgaaaaagat
    12181 gccgccctgt cgcgccgctt ccagttggtg aaggtcagcg aacccaacgc tgccgaagcc
    12241 accattattc tgcgcggtct gtcggcggtc tatgaacagt ctcacggcgt gctgattgat
    12301 gatgacgcct tgcaggccgc tgcgacatta agcgagcgtt atctctccgg gcgtcagtta
    12361 ccggacaaag cgattgatgt gctggatacc gcctgcgccc gtgtggccat caacctgtcg
    12421 tcgccgccga agcaaatctc ggcgctgacc actctgagcc accagcagga ggcggaaatt
    12481 cgccagcttg agcgcgagct tcgcatcgga ctgcgtaccg acacatcacg gatgaccgag
    12541 gtgctggtgc agtatgatga aacgctgacg gcgctggatg aactggaagc ggcctggcac
    12601 cagcagcaga cgctggtccg ggagattatc gcgctgcgcc agcagttact gggcgtggca
    12661 gaggacgatg cggcgccgtt gccggacgca gataccgtgg aggatacgca gccagagtca
    12721 gagtcagagt cagagtcaga gtcagagcag gataataccg gtgccgaacc ggctgatgag
    12781 gccgacagag aacaaccgga agagaccgct gaaacagttt ccccggtaca gcggctggcc
    12841 cagctcactg ccgaactgga cgccctgcat aacgaccggt tgctggtctc cccgcacgtc
    12901 gataaaaaac agattgcggc ggtgattgcc gaatggaccg gcgtgccgct caaccgcctg
    12961 tcgcagaatg aaatgtcggt catcaccgac ctgacgaaat ggctgggtga caccatcaaa
    13021 ggccaggacc tggcgattgc cagcctgcat aagcatctac tgaccgcacg cgccgacctg
    13081 cgtcgtccgg gacgcccgct cggtgcgttc ctgctggctg gccccagcgg tgtgggtaaa
    13141 accgaaaccg tcctgcaact ggcagagctg ctctacggcg gtcgccagta cctgaccacc
    13201 atcaatatgt ccgaattcca ggagaaacac accgtctcgc ggctgattgg ttcgcctccg
    13261 ggctacgttg gctacggtga aggcggcgta ctgaccgaag cgattcgcca gaaaccctac
    13321 tcggtagtac tgctcgatga agtggaaaaa gcgcacccgg atgtgctcaa cctgttctac
    13381 caggcgttcg ataagggcga aatggcagac ggtgaaggcc gcctgattga ctgcaaaaat
    13441 atcgtcttct tcctgacgtc caacctcggc taccaggtaa tagtcgagca tgccgatgac
    13501 ccggaaacca tgcaggaagt acagtatccg gtgctggccg acttcttcaa acctgccctg
    13561 ctggcgcgta tggaagtggt gccgtatctg ccgctgtcga aagagacgct cgccaccatt
    13621 atcgccggaa aactggcccg tctggataac gtgctgcgca gtcgctttgg tgtagaagtg
    13681 gtcattgaac cggaagtgac ggacgaaatc atgagccgcg tcacccgcgc ggaaaacggc
    13741 gcgaggatgc tggaatcggt catcgatggc gacatgctac cgccgctctc gctgctgctg
    13801 ttgcagaaaa tggcggctaa cacggcgatt gcccggattc ggttgtcggc agtggacggc
    13861 gcatttacgg cagacgtgga agatgctcag aacgacgagt ccgtcacaaa ggatgaaacg
    13921 gttttatgag caggttgcgc gttttccggc agacaggatg gtttatcgcg ggcctgatga
    13981 cgggcttacc cgccactgcc gcgcccgcag aagcatcgtc aatggccggt gtggcggtcg
    14041 ccgtcgccac cacgaccccg ccggatgcga cagccacgtt acaggccatg cagtcctgtc
    14101 ggcgggagtc cgcggcgctg gagcggcttg actgctatga ccgcctgctg gcaccgctgt
    14161 ccccgtcagg gtttgacggg gcgctggtca aagccggttt tgtgggcgag gcctggaccc
    14221 gcgccacaga gcaggaaaaa cgccgcgagg gcaacaccac agaactgctg gtgacgcagg
    14281 tgccggggga gcgcccgacg gtggtgataa ccaccccggc tatcggtcat gttccgccac
    14341 gcccggtgct gatgttcagt tgcgtggaca atatcacccg gatgcaggtg gcgctgatgc
    14401 accctctgga tgtccatgat atcgccgtca ctctgaatgc cgacaaccgg gcattgcgca
    14461 gccactggtt tgtgcgcgag aacggcaccc tgctggagtc cagccggggg ttatccggga
    14521 ttgatgaaat taagcaactg ttcggcgcaa aaacgctgac ggtggatacc ggcgcagaca
    14581 atgccgccgg aaagctgacc tttaacattg acgggctggc aagggcgatt gctcccctgc
    14641 gcgatgcctg ccactgggcg ggagaataaa aatatggcaa tggacttacg cgatccgaat
    14701 gtctggatat cgcacctgct ggaaaacctg ccggaagaga aactggcatc ggcgctgaaa
    14761 gatgacaacc cgaactggga gtatatcgac ggcgaaatcg tcaagctggg atcgctggcc
    14821 cacagtcaac tggatatccc ggaactccag cgccgggggc tggtcattct ggcttcggag
    14881 agtaaggatt ttcgtctgct ggcgcatctg ctaagaaccc tgcaacacgc cggtgatcct
    14941 catctcgcat tgcgcctgct ggcgctgtat gtggagcatt actgggcggt ggctgcgccg
    15001 cagaatatgg cgcataaaaa acgctttgcc agccaggtca ttaaacgctt tgaaacaggt
    15061 attgaagttt tttcacagaa cgccgccaca gcgcagcgcg atgcgctgtc aggggaactg
    15121 gcaaaactgg cgcaatgctg gcagtcacat aacgccccgg aactggcaca ggctactgat
    15181 gatctttttg ccctgtacca gcgtgcgttt aatcgtgcgg caccggctcc tgtccccact
    15241 ccggcggcct ccggtagttc accacagacc accgccacgt ctgaaagcgg cgtgacgcaa
    15301 cccagtgctc cggctcccca aatcgtcatc gacagtcacg acgacaaagc ctggcgcgac
    15361 acgctgttaa aagtggcggc cattttatgt gaacgccagc cggattcacc gcagggctac
    15421 cgtctgcgcc gccatgcgct ttggcagaac atcaccagta cgccccaggc ggaaagcgat
    15481 agccgtactc cactggctgc ggtctccgcc gatatggtgg cagattacca tgctcagctc
    15541 ggtagtgccg atatggcgct gtggcagcag gtggagaaaa gcgtactgct ggctccttac
    15601 tggctggatg gtcactgcct ttctgcacag acggcgctgc gtctgggtta caaacaggtg
    15661 gcagacgcca tccacgatga ggtcatccgc ttccttgagc gtctgcccca gcttaccggg
    15721 ctgctgttta atgaccacac accgtttatc agtgagcaga cgaaacaatg gctggcagct
    15781 tcgcccgacg ccaaagttgc accggttgcg caaatcggtg aggaatcgaa ggcagccaga
    15841 gcctgttttg ctgagcaggg tctggaggcg gcgctgcgat atctggacat gctacccgaa
    15901 ggcgatcccc gcgatcagtt tcaccgccag taccttgccg cacagttgac ggaagaggcg
    15961 gggctggtac agcttgcgca gcagcagtac cggatgttgt tcaggatggg attgcagatg
    16021 atggtggcag actgggagcc gtccctgctt gagcagctag aacaaaaatt cacggcagaa
    16081 caataaaaga gaacaggagt ttttgtgttc agattaccaa caccccgact actcagcgga
    16141 ctcaaatcag ccctgcgacc ggcgatgccc cggtttaaag tctctgcttt ctggctgctg
    16201 atactggcgt ggatttttct gctggtgtgg atctggtgga aaggcccgat gtggacgctg
    16261 tatgaagaac agtggctcaa accactggcg aaccgctggc tggcaacggc ggcgtggggg
    16321 attattgccc tggtgtggct taccgtccgg gtgatgaagc gcctgcaaca gcttgaaaaa
    16381 atgcaaaagc aacagcgcga ggaagccgtt gatccgctca gcgtggaact gaacgcccag
    16441 cagcgttatc ttgaccgctg gctgctgcgc ctgcaacgcc atctcgacaa ccgccgtttc
    16501 ctgtggcagt tgccgtggta tatggtcatc ggcccggcgg gcagtggcaa aacgacactg
    16561 ctgcgcgagg ggtttccgtc cgacattatt tatgccccgg agggcgcacg cggcgcagaa
    16621 caacgcctgt acctcacgcc ccatgtcggt aaacaggcgg tgatctttga tatcgacggc
    16681 acactctgcg ctcccgctga tgcggatatc ctgcatcgcc gtctgtggga acatgccctc
    16741 ggctggctga aagaaaagcg cgcgcgccag ccgctgaacg ggattattct gacactcgat
    16801 ttacccgatc tgctgaccgc agacaaacgc cgccgcgagc atctgttaca gacgctgcgc
    16861 agccgcttac aggatatacg ccagcatctt cactgccagt taccggttta cgtggtactg
    16921 acccggcttg atttattaca gggttttgcc gccctgttcc agtcactcaa cagacaggat
    16981 cgcgatgcga ttctgggcgt cacgttcacc cgccgtgccc atgaaaatga tgactggcga
    17041 acagagttga atgctttctg gcagacatgg gtggatcgaa tgaatctggc gttgccggat
    17101 ctgatggtcg ctcagactca cacccgcgcg tctttattca gtttttcccg ccagatgcag
    17161 ggaagccgtg aaccgctggt gtcactgctt gagggtctgc ttgatggcga aaatatgaac
    17221 gtgatgctgc gtggtgtcta tctcacctct tcgcttcagc gtggacagat ggatgatata
    17281 ttcacccagt ctgccgcccg ccagtaccgg ctgggcaata acccactggc gtcctggccc
    17341 ctggtggaca ccgcgcctta tttcacccgc agcctgttcc cgcaggcatt actcgcagag
    17401 cctaatctgg caacagagag ccgcgcctgg ctgatacgtt cccgtcgccg cctgacggtt
    17461 ttctctgcca caggcggcgt ggcagcactg ctgctcatca ccggctggca tcactattac
    17521 aacggtaact atcagtccgg catcaccgtg cttaagcagg ccaaagcctt tatggacgtg
    17581 ccgcctccgc agggggaaga tgactttggc aacctgcaac tgccgctcct gaacccggta
    17641 cgcgatgcca cactggccta tggcgactgg ggcgaccgca gccgtctggc cgatatggga
    17701 ctgtaccagg gacgacgtat cgggccttat gtg
//
LOCUS       DAJTVJ010000088        17438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000088 DAJTVJ010000000
VERSION     DAJTVJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.088"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..502)
                     /locus_tag="OO448_002876"
     CDS             complement(<1..502)
                     /locus_tag="OO448_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6338040.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="OO448_002877"
     tRNA            complement(688..772)
                     /locus_tag="OO448_002877"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="OO448_002878"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="OO448_002878"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6338041.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO448_002879"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO448_002879"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6338042.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="OO448_002880"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="OO448_002880"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCR6338043.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="OO448_002881"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="OO448_002881"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR6338044.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="OO448_002882"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="OO448_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCR6338045.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="OO448_002883"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="OO448_002883"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCR6338046.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="OO448_002884"
     CDS             7117..8619
                     /locus_tag="OO448_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6338047.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO448_002885"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO448_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6338048.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO448_002886"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO448_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6338049.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="OO448_002887"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="OO448_002887"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6338050.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="OO448_002888"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="OO448_002888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6338051.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="OO448_002889"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="OO448_002889"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6338052.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="OO448_002890"
     CDS             complement(16186..17382)
                     /locus_tag="OO448_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6338053.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4332 c         4451 g         4172 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg agggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaa
//
LOCUS       DAJTVJ010000089        17143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000089 DAJTVJ010000000
VERSION     DAJTVJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.089"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO448_002891"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO448_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6338054.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO448_002892"
     CDS             2219..2767
                     /locus_tag="OO448_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6338055.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO448_002893"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO448_002893"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6338056.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO448_002894"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO448_002894"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6338057.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO448_002895"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO448_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6338058.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO448_002896"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO448_002896"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6338059.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO448_002897"
     CDS             complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO448_002897"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6338060.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9768..12380
                     /gene="pepN"
                     /locus_tag="OO448_002898"
     CDS             9768..12380
                     /gene="pepN"
                     /locus_tag="OO448_002898"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6338061.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO448_002899"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO448_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6338062.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OO448_002900"
     CDS             complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OO448_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6338063.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OO448_002901"
                     /pseudo
     CDS             complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OO448_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OO448_002902"
     CDS             complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OO448_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6338064.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16250..16825)
                     /gene="ssuE"
                     /locus_tag="OO448_002903"
     CDS             complement(16250..16825)
                     /gene="ssuE"
                     /locus_tag="OO448_002903"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6338065.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4298 a         4421 c         4350 g         4074 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aaagggagcc
     8281 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     8341 gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt
     8401 tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct
     8461 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     8521 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     8581 tactctgtgg atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa
     8641 ccgggtcgcc agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat
     8701 cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat
     8761 actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt
     8821 gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac
     8881 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga
     8941 tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc
     9001 gcgacatatc aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt
     9061 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     9121 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat
     9181 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta
     9241 accagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     9301 cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac
     9361 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     9421 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     9481 caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta
     9541 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     9601 tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta
     9661 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     9721 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     9781 cacaagccaa ataccgtcac gattatcgtg cgccagatta ccagattact gatattgact
     9841 tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     9901 atggtgcatc agatgctcca cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     9961 atattaatga tgagccgtgg accgcctgga aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcattttacg cttaagatcg ttaatgaaat aagcccggcg gcgaataccg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaaccaaa tatcccttcc tgctttccaa cggtaaccgc gttgcgcaag
    10261 gtgaactgga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgacaccttt accacgcgtt
    10381 ctggtcgcga agtggcgctg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactcaatga aatgggatga agaacgcttc ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccgattgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggca aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagtga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atcgacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcga aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggaggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctg tacgagaaga
    12361 tcaccaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 caatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctactgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctggccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tgtcttcctg aatctcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc tgccagcagg cgcagcaggg
    13201 tacttttgcc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcataccgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtcc gtttgcaaaa actcccgcgc
    13501 attcatcgcc agataaccaa tgcctgaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcaccagg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatttccac
    13681 cagaccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaatagcgt
    13741 gccgagcgcc accagaaaga tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gtacgttgcg caacatctga attgaggtat ccagcagccg
    13861 ttctccccag cgtgacagcc cgctaatcag tcccagaatc aagcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgctgccaca attcgccgct
    13981 ggcggagagc gtccagaacg ccgtcaccac tccttccggt gaaggcaaaa tacgcgtcga
    14041 cagccagcca accgaggagg ccagttgcca caccgccacg atgcccaccg gtaaaaacca
    14101 gggggcaacg cgcaataacc acttcttcac tggcgttgcc atcatcgtgt tccttagctt
    14161 tgcgcgactt tacgggggat aaaatcattc gccaccgctt cgccttgcgg attcagcggc
    14221 tgcggctggg gaatttccgg aatggcgaca tccagatgtg ggaacagcaa ctcgccaacc
    14281 cgatacgctt cttccagatg cgggtagccc gaaagcacaa agctgtcgat gccaagcgcg
    14341 gcgtattcgt tgattcgcgc ggcgaccgta ggaccatcgc ccaccagcgc cgttccggca
    14401 ccgccgcgca ctaagccaac gcccgcccat aaattggggc tgatctccag attgtcgcgc
    14461 ttgccgttat gtagcgccgc cattcgctgt tgtcctacgg aatccgtccg ggcgaatgcg
    14521 gcctgtgctt tggcgatagt ttcatcatca agatgcgaga ttaaccgctc ggcggcctgc
    14581 cacgcttcgt cgttagtttc acgaacaatc acatgcagac gaataccgaa acgaattttg
    14641 cgtccatgcg cggcagcttt cgcccgcact tgttcgatct tctcttttac cagctctggc
    14701 ggttcgcccc aggtgaggta gagatcaact tgttctgccg ccagctcctg ggcgacatct
    14761 gacgatccgc caaagtaaag cggaggatac ggctgttgaa tcggcgggaa gagcagtttt
    14821 gctccccgca catgaatatg tttaccgttg aaatcgacgc tttcccccag caataagcgc
    14881 cccgccagac ctgggtaaat tccgccgagg cttcgtagcg ctcgctatga tcaaggaaca
    14941 cgccgtcgcc tgccaactct tgtggatcgc tgcctgtgac cagattaaac aacgcacgac
    15001 catttgagag acggtcaagc gtggcggcct ggcgagcggc aacggtaggt gaggttacgc
    15061 tgggacgcag ggcgacaaga aacttcagcc gctgcgtcac cgggatcatc gacgccgcca
    15121 ccagccacgc atcttcacac gatcgccccg ttgggatcag cacaccggta tagcccagac
    15181 gatccgccgc ttgcgcaatt tgttgcagat agccgtgatc aaccgggcgt gaaccttctt
    15241 ccgttccaag ataatgcccg tcaccatggg tcggtaaaaa ccagaacata ttcagactca
    15301 taattgtttt ccttccaggt gagtgggctg ccagatgcgc tggcgaatat caactttttt
    15361 cgacaccaga cggttttcat aaaacagatc tgccgtttgc tgctgtaagg cggcaacttc
    15421 agcgctcacc ggtttgatgg tggtgggagg acgatgatcc agatacgagg caatcaccgg
    15481 tgccggtaag cccatcgttt ttgccagtaa agcgatgctt tgctcgcgct ggctgcgggt
    15541 taacgcatcg gcctcactaa aggttgccag tacgccctga ataaaagcgc cgtttttttc
    15601 tgcatagggg cgagctgcca gataaaacga tccagtttga ttgagatcgg tgccgtcttt
    15661 cagcacccgc acgccgccct gtaataacgc agcggagtag tagggatccc agatagccca
    15721 ggcgtcaacg ttaccttgct ggaacgcggc gcgggcatca gctggcgtca gataagtggg
    15781 ctggatgtca gtaaacttaa gtccggcctg acgcagtgca cgcagtaaaa ggttgtgtga
    15841 actggaacct ttctgaaagg caactttgtg acctttaaga tcggctacgg ttttgatcgg
    15901 gctgttttct gccaccagaa tcacttcggc tttgggcttc ggtggctcga cgcccacata
    15961 tatcaaatca gccccggcag cctgggcaaa gattggcgga atatccccgg tactgccgag
    16021 atcaatactg ccaacgttta acgcttccaa catttgcgga cccgcgggga actccaccca
    16081 ggagattttt gattgcggat agcgtttttc cagtaactgg tggctttttg ccaataccat
    16141 accaatactg cctttctgat agcctatacg taacgcttca ggcgaggatt ctgcagcaac
    16201 cgcaaaagta gagacgctaa gcaatcccgc cagcgccagt ttaatgatgt tacgcatgga
    16261 cattacctcg cagagacaga aggtcaggaa cctgaacatc gcggcggtgc aatgcctgcc
    16321 agaaagtttc tagcgcggta tcaagacggg tttgcagatt tggcgtgaac tggggtttgt
    16381 gatggtaatc aataacttgt gagtcatcgg caaacacgcc gtgcaggagc tcctgggctt
    16441 tcatcgcact taaaactggc ttaagggcat aatcgaccgc cagcagatga gccaccgtac
    16501 cgcccgtcgc cagcggtagc accactttgc cttgcaaagc gcgctctggc agcaggtcga
    16561 gcagggtttt cagcgcgccg gaataggcgg ctttatacac aggtgtagcg actatcagtc
    16621 catcagcctg ttgcagctgt tcggtgaagg tcttgagtgc cggactatcg aaacgagcgt
    16681 aaagcagatc ttccggggcg aagttttgca gattccagtg ataaacctct acatccaggc
    16741 catttagttt ttcccgcgca tattccagca aggagctgga gcgagaagga aagcgaggac
    16801 tacccgccag ggtgatgaca cgcatactct ctccttataa ccaattgttc tttttttgtt
    16861 aacattgata acaattcggt cagtctgtcg gagagacaag aaaatgccaa atataaattt
    16921 tgtgtatctt tttctgaaaa taaactaaag aaagggcttt atatatcacg catatttatt
    16981 tgttggatag tcattagatg ttgaatcaat gaattactcg ttcagttaat atttaaatat
    17041 tcatgaaatc tataaattaa agatttgtca cttattggat ttagtatgct tgatttaaat
    17101 ggcggtcaat tattgttatt attaagtcta atgtcattta aag
//
LOCUS       DAJTVJ010000090        16646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000090 DAJTVJ010000000
VERSION     DAJTVJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.090"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            79..2394
                     /gene="bisC"
                     /locus_tag="OO448_002904"
     CDS             79..2394
                     /gene="bisC"
                     /locus_tag="OO448_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6338066.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2363..2803)
                     /locus_tag="OO448_002905"
     CDS             complement(2363..2803)
                     /locus_tag="OO448_002905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6338067.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2800..3363)
                     /gene="tag"
                     /locus_tag="OO448_002906"
     CDS             complement(2800..3363)
                     /gene="tag"
                     /locus_tag="OO448_002906"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6338068.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3521..4219
                     /locus_tag="OO448_002907"
     CDS             3521..4219
                     /locus_tag="OO448_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6338069.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4448..5656
                     /locus_tag="OO448_002908"
     CDS             4448..5656
                     /locus_tag="OO448_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6338070.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5980..7671
                     /gene="eptB"
                     /locus_tag="OO448_002909"
     CDS             5980..7671
                     /gene="eptB"
                     /locus_tag="OO448_002909"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6338071.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7763..7839
                     /locus_tag="OO448_002910"
     tRNA            7763..7839
                     /locus_tag="OO448_002910"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7797..7799,aa:Pro,seq:cgg)
     gene            7874..8007
                     /locus_tag="OO448_002911"
     ncRNA           7874..8007
                     /ncRNA_class="other"
                     /locus_tag="OO448_002911"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8752..10359
                     /gene="dppA"
                     /locus_tag="OO448_002912"
     CDS             8752..10359
                     /gene="dppA"
                     /locus_tag="OO448_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6338072.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10667..11686
                     /gene="dppB"
                     /locus_tag="OO448_002913"
     CDS             10667..11686
                     /gene="dppB"
                     /locus_tag="OO448_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6338073.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11696..12598
                     /gene="dppC"
                     /locus_tag="OO448_002914"
     CDS             11696..12598
                     /gene="dppC"
                     /locus_tag="OO448_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6338074.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12609..13592
                     /gene="dppD"
                     /locus_tag="OO448_002915"
     CDS             12609..13592
                     /gene="dppD"
                     /locus_tag="OO448_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6338075.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13589..14593
                     /gene="dppF"
                     /locus_tag="OO448_002916"
     CDS             13589..14593
                     /gene="dppF"
                     /locus_tag="OO448_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6338076.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14623..15894)
                     /locus_tag="OO448_002917"
     CDS             complement(14623..15894)
                     /locus_tag="OO448_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6338077.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16370..16477
                     /locus_tag="OO448_002918"
     CDS             16370..16477
                     /locus_tag="OO448_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6338078.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         3962 a         4144 c         4487 g         4053 t
ORIGIN      
        1 attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa tacatctaac
       61 ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct gactgccgcc
      121 cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg
      181 ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt tcacagcaat
      241 acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga aaacccgcaa
      301 ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct ggatcttatt
      361 caccaacaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt tgctggttcc
      421 tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca acgctatatg
      481 gcgctggcag gcggttatac cgggcatctg ggggattatt cgaccggtgc ggcacaggcg
      541 atcatgccgt atgtcgtagg tggcagcgaa gtttatcaac agcaaaccag ttggccgctg
      601 gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa tacgctgaaa
      661 attgcgtgga atgcatccga tgagcagggg ctttcttact tttccgcact gcgtgacagt
      721 gggaaaaagc tgatctgcat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat
      781 aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc
      841 catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg caccacaggt
      901 tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagcgaa aaccgccgaa
      961 tgggcagcag agatttgtgg tgttggcgca gcgaaaatcc gccagctggc ggctattttc
     1021 caccaaaata ccaccatgct gatggcaggc tggggaatgc agcgccaaca gtttggtgag
     1081 caaaaacact ggatgatcgt cacgctggca gcaatgttgg ggcaaatcgg cacacccggc
     1141 ggcggttttg gtctttctta ccattttacg cggcgttctg cggtgctctc ttccatgcag
     1201 ggcagcttgc cgggtggctg cgatgcggtg gataaaatcc ctgttgcccg cattgttgaa
     1261 gcactggaaa accctggtgg cgcatatcaa cacaacggta tgaaccgaca tttcccggat
     1321 attcgtttta tctggtgggc gggcggtgcc aactttactc atcatcagga taccaatcgt
     1381 ctgatccgtg cctggcaaaa accggagctg gtggtgatct ctgaatgctt ctggacggcg
     1441 gcggcgaaac acgcggatat cgttctgcct gcgactacct cttttgagcg taatgatctc
     1501 accatgaccg gtgattacag taatcagcat ctggtgccga tgaagcaagt ggtgccgcca
     1561 cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt tgagtgaacg ttgggagaag
     1621 ggcggctatg cgcggtttac ggaaggaaaa agtcagctgc aatggctgga aacgttttat
     1681 aacgttgccc ggcagcgcgg ggcaagccag caggttgaat tgccgccatt tgctgagttc
     1741 tggcaagcca accagttaat tgagatgccg gaaaacccgg acagcgagcg gtttattcgc
     1801 ttcgctgatt tttgccgcga tccgctggcg catccgttaa aaaccgccag cggcaagatt
     1861 gaaatcttct cacagcgtat tgccgattac ggttacccgg attgccctgg gcatccaatg
     1921 tggctggagc cggacgaatg gcagggcaat gccgaaccag aacagttgca ggtactttct
     1981 gcccatccgg cgcaccgcct gcacagccag ctgaattaca gttctctgcg cgaattgtac
     2041 gcggtagcaa accgtgagcc tgtcaccatt catcctgacg atgctcaggc gcgcggcata
     2101 accgaagggg atatggtgcg ggtgtggaac tcccgtgggc agatccttgc cggagcggtc
     2161 attagcgagg gaattaaacc tggcgtgatt tgcattcacg aaggggcatg gccggatctg
     2221 gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc
     2281 cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg cgctggcatg gctggaaaaa
     2341 tacaacggtc cggaactgac acttacagcg tttgaaccac cggccagctc ataatccagg
     2401 tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt
     2461 aaaaatctat cgccggttga tttttttgat aaacctccag catcagatgg ggatagcgct
     2521 gctgcacata ctgcatcagc gccttaccaa taccgcgtct gacggccttc ggtgcgacaa
     2581 acattgctgc cagaaatcgg ccttccataa tgctgacaaa accgagaagc ttaccgtcct
     2641 cttcccagac ccagttttgc gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt
     2701 cacgccagta attcgctttt ataaagggat gcccccaggt tgtactttcc agccacagtt
     2761 cgaggatcgc ggggagttct gaacgttgcg cttcccgaat catggtttat ttcccggata
     2821 gcaacagcag ccaaccacat gatcattcac cagcccacat gcctgcataa aggagtaaca
     2881 gattgtggtg ccgacaaact taaaaccacg ttttttcagt gctttagata gggcgtcgga
     2941 ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg
     3001 atttacaaac gaccagacaa agtcggcaaa cggttcgccg ttctgttcca tttgcaggta
     3061 cgcccgcgca ttaccaataa ttgcctgaat tttccctcga tggcggataa tcccggcgtc
     3121 ctgtaccagt ctttcgacat cctcttcctg cattgctgcg accttcaccg gatcgaactg
     3181 atgaaagcag gcgcgatagt tttcgcgttt tttgaggacg gtgatccacg ataacccagc
     3241 ctgctgccct tcaaggcaga tcatttcgaa cagtttttta ctgtcagttt caggcacgcc
     3301 ccattcatta tcatggtagg caatataaag cgggtcctga ctcacccagc cgcaacgttc
     3361 catactttcc ctcgctatgt acccgatcgt aaaaatttca gccgcttttc ttgacgcatc
     3421 ggtgaaaaag tcaatattta atacagggct actatgcccg atatccttca tacaatgaca
     3481 ataatattgc cgaattcggc tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg
     3541 tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc agtgcctttt ttctcaacac
     3601 agcttacgcc tggcaacaag aatatatcgt tgatacgcaa cccggacatt ccacagagcg
     3661 ttacacctgg gatagtgatc atcaacctaa ttacaacgat attttgtcgc aacgtattca
     3721 aagtagccaa agggcgctgg gactggaagt caatctggcg gaagaaactc ctgtggatgt
     3781 gaccagcagt atgagtatgg gctggaattt tcctttatat gaacaggtta caaccggccc
     3841 ggtcgcggca ttacattacg atggcacaac cacctcgatg tataacgagt ttggcgacag
     3901 tactaccacg cagaccgatc cgttatggca tgccagcgtg agtaccttag gctggcgtgt
     3961 tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc agctataacc agcaatttgg
     4021 cgagaatatc tggaaggcgc aatcaggcct gagccggatg acggcgacaa accagaacgg
     4081 caactggctg gatgtcaccg taggcgctga tatgttgctc aatcaaaata ttgccgccta
     4141 tgccgcgcta actcaggcag aaaataccac taataatagc gactatctgt atacgatggg
     4201 ggttagcgcc agattttaac gtaacagtca caattgaaac cattaaataa caataattgt
     4261 ggcgatagtg ggtgctaact taccaaataa taaatttggt gaataattgt cgcgtcattc
     4321 attcctgaac taaggcattt cattccgttc tgatggcatt tcatgccgtt tttccccagg
     4381 cataaagtgc acttcgttat ggttgtcggc agagattttt cctttttatt actgcaggaa
     4441 tactgccatg acaccttcaa attatcagcg tacccgctgg ctgacactca tcggtactat
     4501 cattacccag tttgcgctgg ggtcggttta tacctggagc ctgtttaatg gcgcgctttc
     4561 cgccaagctg gatgcgccgg taagccaggt cgctttctct ttcggcttgt taagtctggg
     4621 gctggcaatt tcgtcttctg ttgcgggcaa attacaggaa cgtttttgcg ttaaacgcgt
     4681 caccatggct tccggcattt tgctgggatt aggcttcttc ctgaccgcgc attccaacaa
     4741 cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta
     4801 cctgctgacg ctctctaact gcgtgaagtg gttcccggag cgtaaaggtc tgatctccgc
     4861 gttcgctatc ggttcttatg gtctgggtag cctgggtttc aaatttatcg acacgcagct
     4921 gctcgaaacg gtgggtctgg aaaaaacatt tgtgatttgg ggagcgattg cgctggtgat
     4981 gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa
     5041 tggtgtggtg gagaaagatt acacgctggc agagtcgatg cgtaaaccgc agtactggat
     5101 gttagcggta atgttcctga ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa
     5161 agatatcgcc caaagtctgg cacaccttga tgtggtttcc gcagccaatg cagtaacggt
     5221 tatttccatc gctaaccttt caggtcgtct ggtgctgggc attctgtctg acaaaattgc
     5281 ccgtatccgt gttatcacca ttggtcaggt gatttcgctg gtgggtatgg cggccctgct
     5341 gtttgcacca ttaaatgcag tgacgttctt tgcagcgatt gcctgcgtgg catttaactt
     5401 tggcggcact attaccgtct ttccatcact ggtcagtgag ttcttcggcc tcaataacct
     5461 ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc ggtagcattt ttggttcgat
     5521 tatcgcctca ctgtttggcg gtttctatgt gaccttctac gtgattttcg ccctgctgat
     5581 tctgtcattg gcgctttcta cgacgattcg tcagccagag cagaaaatgt tgcgtgaggc
     5641 gcatggctcc ctttaatgct cagccgcgag ctactgggct tcagcttccg tgtgcatcaa
     5701 acgcatcaac gaaatgtata aacaatgact cgtaaaattt gggccagtta actggccctt
     5761 tttactgctt tttgtaacgc taaagtctct tttcaaactt gcatttttgt aaatttgtgc
     5821 ttcatgcaca ctctttcccc acactttttc gctttgctgt ggtctactta ttcgcgcgtg
     5881 tagattttac ttatctgact acctccgcac ttttcccctg ccgggcctga aaagccgtta
     5941 agcagggtgt tatcacctgt ttgtccaggg tttgtttgca tgagatacat caaatcgatt
     6001 acacagcaga agctgagctt tttgcttgca atctatattg gcctttttat gaatggcgcg
     6061 gttttttatc gccgcttcgg cagctatgcg cacgatttta ccgtctggaa aggcgtttct
     6121 gctgttgttg aactggccgc caccgtactg gtgaccttct ttttactacg tcttctatcg
     6181 ctgtttggcc gccgcagctg gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt
     6241 gccagctatt acatgacctt ccttaatgtg gtcattggtt atggcatcat cgcttccgtc
     6301 atgaccaccg atatcgacct gtcaaaagaa gttgttggtc tgaactttat tctctggtta
     6361 atcgccgtta gtgcattgcc tcttatcctt atctggaata accgctgtcg ctacaccttg
     6421 ctccgacaac tgcgaacccc agggcagcgt attcgcagcc tggcggtcgt cgtactggcg
     6481 ggtattatgg tttgggcacc gattcgtttg ctggatatcc agcagaagaa agtggagagg
     6541 gcgaccggcg ttgatttgcc gagttatggc ggtgtcgtag cgaactctta tctgccatca
     6601 aactggcttt ctgcgttggg gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat
     6661 aattcattgc ttaatccggc gaagaagttt acataccagg ctccacaaaa cgttgatgag
     6721 acttatgtcg tgtttatcat cggtgaaacc acgcgttggg accatatggg tattttcggc
     6781 tatgagcgta ataccacgcc gaaactggcc caggagaaaa atctggcggc gttccgtggt
     6841 tactcctgtg ataccgcaac caaactctca ctgcgttgca tgtttgtacg tcaggggggc
     6901 gcggaagata atccgcagcg cacattaaaa gaacagaaca ttttcgcggt tctgaagcag
     6961 ttaggattca gttctgacct ctacgctatg cagagcgaaa tgtggttcta cagcaacacg
     7021 atggcggaca acattgctta tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag
     7081 ccggtagatg atatgttgct ggtagacgaa atgcagcaat cgctagggcg caacccggat
     7141 ggtaagcatc tgatcattct gcataccaaa ggttcgcatt ttaactacac ccagcgttat
     7201 ccgcgtagct tcgcgcagtg gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa
     7261 gcgcagatga tcaactccta tgacaactcg gtgacctatg tggatcactt tatctccagc
     7321 gtgattgatc aggttcgcga taagaaagcg attgtgttct acgcagctga ccacggtgag
     7381 tcaattaatg aacgcgagca cctgcacggc acgccgcgtg aactggcacc gccggagcag
     7441 ttccgcgtac cgatgatggt ctggatgtca gataaatatc tggaaaatcc ggccaatgcg
     7501 caggcgtttg cgcagctgaa aaaagaagcc gacatgaaag tgccacgccg tcacgtagag
     7561 ctgtacgaca ccatcatggg ttgtcttggc tatacttcac cggatggtgg aattaacgaa
     7621 aacaacaact ggtgtcacat cccgcagaca aaagaggcag cggctaacta agaggcctgc
     7681 tgactttctc gccgatcaaa aggcattttg ctattaaggg attgacgagg gcgtatctgc
     7741 gcagtaagat gcgccccgca ttcggtgatt ggcgcagctt ggtagcgcac ttcgttcggg
     7801 acgaaggggt cggaggttcg aatcctctat caccgaccaa attcgaaaag cctgctcagc
     7861 gagcaggctt ttttgcattt gaagcccatg aggatgagaa cctccggggg cggggaggtt
     7921 cgactcgagc gaagcgagag aacgttgcgc cagcaacggc ccgcagggcg agccacgaag
     7981 tggcgagtaa tcctctatca ccgaccaaat tcgaaaagcc tgctcaacga gcaggctttt
     8041 ttgcatttgt agctcatgag gatgagaacc tccgggggca gggaggttcg actcgacgta
     8101 ggcctgataa gacgcgccag cgtcgcatca ggcaacggca ccgcatttat tgtcggatgc
     8161 ggcgcgaccg ccttattcga ccaacacacc acacattttc ctgccaactt tgcgccccat
     8221 atctcactat ccattaacgg atttgtgaca gaaaccattg tcttttttta tatttgctta
     8281 aatctttttt tgcgttactt atggctggcg ctggcatttt ccgctgtgcg ttttagcgtt
     8341 catggtatta atcgctcaga tatccacctt ttcctcagat ttttttcatt aaatgcataa
     8401 aacttaatca ccaaatgttg ctaaatatta agctgattgt tctggttatg ttggagtagc
     8461 acaaatttct ctgctgcaaa tagctgtttg aagttttttt aatctttgtt tgtggattct
     8521 caccgttggt gtaaatcccg gttggctgta ttgacgtttt cacattctgt tgacagattg
     8581 taggtcacga ggggcatttt atggaggatc cgcactgtta cactgatgtt aattagtacg
     8641 gcatccccac ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt caggcagcga
     8701 caacccactg caaagggtta aaacaacaaa catcacaatt ggagcagaat aatgcgtatt
     8761 tccttgaaaa agtcagggat gctgaagctt ggtctcagcc tggtggctat gaccgtcgca
     8821 gcaagtgttc aggctaaaac tctggtttat tgctcagaag gatctccgga agggtttaac
     8881 ccgcagctgt ttacctccgg caccacctat gacgcctctt ccgtaccgct ttataaccgc
     8941 ctggttgaat ttaaaatcgg caccactgaa gtgatcccgg gcctcgctga aaagtgggaa
     9001 gtcagcgaag acggtaaaac ctataccttc catctgcgta aaggtgtgaa gtggcacgac
     9061 aataaagaat tcaaaccgac gcgtgaactg aacgccgatg acgtggtgtt ctcgttcgac
     9121 cgtcagaaaa acgcgcaaaa cccgtaccat aaagtctctg gcggcagcta cgaatacttc
     9181 gaaggcatgg gcttgccgga gctgatcagt gaagtgaaaa aggtggacga caacacagtt
     9241 cagttcgtac tgactcgccc ggaagcgccg ttcctcgctg atctggcaat ggacttcgcc
     9301 tctattctgt caaaagaata tgctgatgcg atgatgaaag ccggtacacc ggaaaaactg
     9361 gacctcaacc caatcggaac cggtccgttc cagttacagc agtaccaaaa agattcccgt
     9421 atccgctaca aagcgtttga tggctactgg ggcaccaaac cgcagatcga tacgctggtt
     9481 ttctctatta cccctgatgc ttccgtgcgt tacgcgaaat tgcagaagaa tgaatgccag
     9541 gtgatgccgt acccgaaccc ggcagacatc gctcgcatga agcaggataa atccatcaac
     9601 ctgatggaaa tgccggggct gaacgtcggc tacctctcct ataacgtgca gaaaaaaccg
     9661 ctggatgacg tgaaagttcg ccaggcactg acctacgcgg tgaacaaaga cgcgatcatc
     9721 aaagcggttt atcagggcgc gggtgtatca gcgaaaaacc tgatcccgcc aaccatgtgg
     9781 ggctataacg acgacgttca ggattacacc tacgatcctg aaaaagcgaa agccttgctg
     9841 aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt gggcgatgcc ggtacaacgt
     9901 ccgtataacc cgaacgctcg ccgcatggcg gagatgattc aggcagactg ggcgaaagtg
     9961 ggcgtgcagg ccaaaatcgt cacctacgaa tggggtgagt acctcaagcg tgcgaaagat
    10021 ggcgaacatc agacggtaat gatgggctgg actggcgata acggggaccc ggataacttc
    10081 ttcgccaccc tgttcagctg cgccgcctct gaacaaggtt ctaactactc aaaatggtgc
    10141 tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca taacaaacgc
    10201 gttgaactgt acaagcaagc gcaggtggtg atgcacgatc agactccggc actgatcatc
    10261 gctcactcca ccgtgtttga accggtacgc aaagaagtca aaggctatgt ggttgatcca
    10321 ttaggcaaac atcacttcga aaacgtctct atcgaataat taaaagccat acaagactga
    10381 tggcaaaggc aaaactgcct gatgtgctcc gcttatcagg cctacgaaaa ttctgcaatg
    10441 tattgaattt gcacgatttt gtaggccgga taaggcgtta acgccgcatc cggcataaac
    10501 aaagcgcact ttgtcaacaa tctgtatacc cggtggcgat gtgcctctgc gtgcagcgcc
    10561 atccggcagc aatacttcat tccctgtccg ataagggcgg gaattgattt gtgagcaata
    10621 cagacacgca gttccaggct gcgggtcact atagagaatc cgggttatgt tgcagtttat
    10681 tctccgacgt ttgggactcg tcatccccac gtttatcggt attacccttc tcacatttgc
    10741 ctttgtccac atgatcccgg gcgatccggt gatgatcatg gcaggcgaac gcgggatctc
    10801 ccctgagcgt cacgcgcagc tgctggctga actcggctta gataaaccga tgtggcagca
    10861 gtatctccat tacatttggg gcgtaatgca cggcgatcta ggcatttcaa tgaaaagccg
    10921 aattccagta tgggaagagt tcgtgccgcg ctttcaggcc acgctggaac ttggcgtctg
    10981 cgcgatgatt tttgctacgg cagtcggtat tccggttggt gtgctggctg cggttaaacg
    11041 cggttccatt ttcgatcaca ctgcagttgg cctggcgctg accggctact cgatgccgat
    11101 cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg cactggaacc tgacgcccgt
    11161 ctccggtcgc gtgagcgata tggtgttcct cgatgactcc aatccgttaa ccggttttat
    11221 gctgatcgac accgccatct ggggtgaaga tggcaacttt atcgacgccg ttgcccatat
    11281 gatcttaccg gctattgtgc tgggtactat cccgctggcg gttatcgttc gtatgacgcg
    11341 ctcttcgatg ctggaagtgc tgggtgaaga ttacatccgc accgcgcgcg ccaaagggtt
    11401 gacccgcatg cgggtgatta tcgtccatgc gctgcgtaac gcgatgctgc cggtagtgac
    11461 cgttatcggc ctgcaggtgg gaacattgct ggcgggggcg attctgaccg aaaccatctt
    11521 ctcgtggccc ggtctgggac gctggttgat tgacgcactg caacgccgcg actatccggt
    11581 agtgcagggc ggcgtattgc tggtggcgac gatgattatc ctcgtcaacc tgctggtcga
    11641 tctgctgtac ggcgtggtga acccgcgtat tcgtcataag aagtaagggg acatcatgtc
    11701 acaggttact gaaaataaag tgattagcgc accggtgccg atgaccccgt tacaggagtt
    11761 ctggcactat tttaaacgca acaaaggcgc ggttgtcggg ctggtttaca tcgtcatcgt
    11821 gctgtttatc acgatctttg ccaactggat tgcgccttat aacccggcgg atcagttccg
    11881 cgatgcgctg ctcgccccgc cagcctggca ggaaggcggc agcatggcgc acttgctggg
    11941 caccgatgac gtaggccgtg atgtgctgtc gcggctgatg tacggcgcgc gcttgtcgct
    12001 gctggttggc tgtctggtgg tggtgttatc gctgattatg ggcgttattc tcggcctgat
    12061 cgccggttac ttcggcggcc tggtcgataa catcatcatg cgcgtggtcg atatcatgct
    12121 ggcgctgcca agtctgctgt tggcactggt gctggtggcg attttcggcc cgtcgattgg
    12181 taacgccgcg ctggcactga ccttcgttgc cttgccgcac tatgtgcgct taacccgcgc
    12241 cgccgtgctg gtggaggtga atcgcgatta cgttaccgcg tctcgcgtgg caggtgccgg
    12301 ggcgatgcgt cagatgttta ttaacatctt cccgaactgc ctggcgccgc tgattgttca
    12361 ggcgtcgctc ggtttctcta acgccattct cgatatggcc gcccttggct tcctcggcat
    12421 gggagcgcag ccgccaacac cagagtgggg caccatgctc tccgacgtgt tgcagttcgc
    12481 gcaaagcgcc tggtgggtcg tgaccttccc gggtctggcg atcctgctga cggtgctggc
    12541 atttaacctg atgggtgacg gtctgcgtga cgcgctcgat cccaaactga agcagtaaga
    12601 ggttcgagat ggcgttatta aatgtagata aattatcggt gcatttcggc gacgaaagcg
    12661 caccgttccg cgccgtagac cgcatcagct acagcgtaaa acagggcgaa gtggtcggga
    12721 ttgtgggtga gtctggctcc ggtaagtcgg tcagttcact ggcgattatg gggctgattg
    12781 attatccggg ccgcgtaatg gcggaaaagc tggtgtttaa cggtcaggat ttgcagcgta
    12841 tatcagaaaa agagcgccgc aacctggtgg gtgccgaagt ggcgatgatc ttccaggacc
    12901 cgatgaccag ccttaacccg tgctacaccg tgggtttcca gatcatggaa gcgattaagg
    12961 tgcatcaggg cggtaataag agcactcgcc gtcagcgggc gattgacctg ctgaatcagg
    13021 tcggtattcc cgatccggca tcgcgtctgg atgtttaccc gcatcagctt tccggcggca
    13081 tgagccagcg cgtgatgatc gccatggcga ttgcctgtcg gccaaaactg ctgattgccg
    13141 atgagccgac cactgcgctg gacgtgacca ttcaggcgca aatcatcgaa ctgttgctgg
    13201 agctacagca gaaagagaac atggcgctgg tgttaattac ccatgatctg gcgctggtgg
    13261 cggaagctgc gcataaaatc attgtgatgt atgcaggcca ggtggtggaa acgggcgatg
    13321 cgcacgccat cttccatgcg ccgcgtcacc cgtatactca ggcattgctg cgtgcgctgc
    13381 cagaatttgc tcaggacaaa gaacgtctgg cgtcgttgcc aggtgtcgtt cccggcaagt
    13441 acgaccgccc gaacggctgc ctgcttaacc cgcgctgccc ctatgccact gacagatgtc
    13501 gcgctgaaga accggcgctg aatatgctcg ctgacgggcg ccagtccaaa tgccattacc
    13561 cacttgatga tgccgggagg ccgacactat gagtacgcaa gaggccaccc tgcaacaacc
    13621 gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg aagaaaggca tgttcgcgcc
    13681 ggaacgtctg gttaaagcgc tggatggcgt ttcgtttaac cttgaacgtg gcaaaacgct
    13741 ggcagtagtg ggcgaatctg gctgcggtaa atcgaccctc ggtcggttgc tgacgatgat
    13801 tgaaacgccc accggtggcg agctgtatta ccaggggcag gatctgctta agcacgatcc
    13861 gcaggcgcag aagctgcgtc ggcagaaaat ccagatcgtc ttccagaacc cttacggttc
    13921 gctgaatccg cgtaaaaaag tcgggcaaat tcttgaagag ccgctgctga tcaacaccag
    13981 cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg atggcgaaag tcggcctgaa
    14041 aaccgagcac tatgaccgct atccgcatat gttctccggc ggtcagcgtc agcgtatcgc
    14101 catcgcccgt ggtctgatgc tcgacccgga tgtggtgatt gccgatgaac cggtttccgc
    14161 gctggatgtt tcagtgcgcg cgcaggtgct gaatctgatg atggatttgc agcaggagtt
    14221 ggggctgtct tatgtcttta tctcccacga cctgtcggtg gtggagcaca ttgctgatga
    14281 agtgatggtg atgtacctgg gccgctgcgt ggagaaggga acgaaagacc aaatcttcaa
    14341 taacccgcgc catccgtaca ctcaggcgct actttccgcg acgccgcgcc tgaacccgga
    14401 cgatcgccgc gagcgcatca agctcagcgg tgaactacca agcccactga atccaccgcc
    14461 gggttgcgcc ttcaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc agttgcagcc
    14521 gcagctaaaa gactacggcg gtcaactggt agcttgtttt gctgttgatc aggatgaaaa
    14581 tccgcagcgt taactcacaa aaccggggcc atgcgctccg gtttactcaa tcagcttcaa
    14641 cagtggcgac aagcatagca aaatgccgta cagcagaatc agccaggctt taaatccgcg
    14701 aagtttttcc agttgtgcga ctttatagat caggaaaaac ggaatgaggc acgacacaat
    14761 accatataac gggcttccca actgaaagaa caccagcacc gatacacgaa acgaaaccca
    14821 aatcgtcaac gtaatgacga taaaagcgca gatcgccaga gtcagcacgc gtgagttaat
    14881 tttcttggta tcaataattc ggcttaacag attgagaata atgcctttaa tggcctcgtg
    14941 gaaaccgaga taaatgccaa agaatgcggt cagtacggca aagatattaa gcaccgtaga
    15001 ggtgatatga atgatatgcc cagggatcac ctgcgcggcc agcgccagtg ctgagatatt
    15061 ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg aaaaggcaaa
    15121 aaacaggatc accgcgatga gcgtgatata actaattcgg tgggtacgca gcgcgagccg
    15181 ggttgccagt actttatccg cttcccgttt acgataggca atattcattg ggttaagtac
    15241 ctgaataaat actgcagaaa agaagcaaaa tggaatggta agcaagacat cgcggaaaaa
    15301 gacggaggct tgcgggaagg cggttatatt ggcgaaattc cagtgcggga tcatcgcaaa
    15361 accgaacacg acaataatcc ctactttgac caccaccatt ggcccggaaa tcttaaacag
    15421 taatcgttca ccaccagacg caatcgctac cagtacggcg aaaatagcga ctttataaag
    15481 tagagattgt gaaagatcgg catcggttaa accgaaggtt ttcaggtacg aggcgctgtc
    15541 gaaaaccacg gagagagagt agataaaaat cccgtggata atcatcaaaa agtagataac
    15601 cccgaggaaa attccccagt tcttccccag gtaatgactg ataatatcgg tgtagtcatt
    15661 acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc aggtggcagg
    15721 ataagcaatg atcgctgcgg taataaatac ccaaattccc ttcaagccaa tttgtactgg
    15781 catcagcacg gttccggcac caatcgccat gcctatgcat aataaaaccc agccgaagtc
    15841 gtagcgtgta aacggcaatt tctgattgtg tttcgatagt gtgttgtgct gcataaagtc
    15901 ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct tgcgcaacag
    15961 gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa cagattcact ggggaggtcg
    16021 cgaggaggcg caattctaca aaattgcgca tttcactacc agtctggagt cagcccggat
    16081 atgaactgca actattgcaa ataaagaaat taagctggaa ggcatcgcgc aatgaggaag
    16141 attgccgggt gctgacagta aaaagcaatc gtaaaatcag cggcaagccg aaagggttcc
    16201 ggtgaggcgc aatgttgcgg gggctttatc cctggtggca ttggttgctg gagagagaaa
    16261 acccccgcac gttgcaggta tgtacctgac aacataacgg gggctaatct tgaacctaga
    16321 ccactcaaga atagccgcga aacgttgtca ttacaacaca ggcggctata tgacgctcgc
    16381 agagctgggc atggccttct ggcatgattt agcggctccg gtcattgctg gcattcttgc
    16441 cagtatgatc gtgaactggc tgaacaagcg gaagtagcgt gtcatgtggg cgtcaggctg
    16501 ccgtaatggc aatttgcgcc cggaccaggc cgcaggagtg aaactctgcg gcctttttcg
    16561 taagaaaaat gcaacggttg caagcggata atattctgcg gaatgcgcag cacagagtga
    16621 agatgagacg gattccggaa cagaaa
//
LOCUS       DAJTVJ010000091        16341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000091 DAJTVJ010000000
VERSION     DAJTVJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.091"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..578
                     /locus_tag="OO448_002919"
     CDS             <1..578
                     /locus_tag="OO448_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6338079.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="OO448_002920"
     CDS             581..1744
                     /locus_tag="OO448_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6338080.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="OO448_002921"
     CDS             1828..3453
                     /locus_tag="OO448_002921"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6338081.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OO448_002922"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OO448_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6338082.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OO448_002923"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OO448_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6338083.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="OO448_002924"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="OO448_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6338084.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OO448_002925"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OO448_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6338085.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OO448_002926"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OO448_002926"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6338086.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="OO448_002927"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="OO448_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="OO448_002928"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="OO448_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6338087.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="OO448_002929"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="OO448_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6338088.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="OO448_002930"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="OO448_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6338089.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="OO448_002931"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="OO448_002931"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6338090.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="OO448_002932"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="OO448_002932"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6338091.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="OO448_002933"
     CDS             12037..12588
                     /locus_tag="OO448_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR6338092.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="OO448_002934"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="OO448_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="OO448_002935"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="OO448_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6338093.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="OO448_002936"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="OO448_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6338094.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="OO448_002937"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="OO448_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6338095.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="OO448_002938"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="OO448_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6338096.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="OO448_002939"
     CDS             complement(14856..15827)
                     /locus_tag="OO448_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR6338097.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..16296)
                     /locus_tag="OO448_002940"
                     /pseudo
     CDS             complement(15880..16296)
                     /locus_tag="OO448_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3839 a         3987 c         4342 g         4173 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgacattgc ccatgacggt aatgactcca acttattgat
    16321 agtgttttat gttcagataa t
//
LOCUS       DAJTVJ010000092        16181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000092 DAJTVJ010000000
VERSION     DAJTVJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.092"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO448_002941"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO448_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6338098.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="OO448_002942"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="OO448_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6338099.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIEGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="OO448_002943"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="OO448_002943"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6338100.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="OO448_002944"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="OO448_002944"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6338101.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="OO448_002945"
     CDS             complement(5254..6294)
                     /locus_tag="OO448_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6338102.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="OO448_002946"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="OO448_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6338103.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="OO448_002947"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="OO448_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6338104.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="OO448_002948"
     CDS             7229..7669
                     /locus_tag="OO448_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6338105.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="OO448_002949"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="OO448_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6338106.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="OO448_002950"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="OO448_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6338107.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..10832
                     /gene="rsxC"
                     /locus_tag="OO448_002951"
     CDS             8898..10832
                     /gene="rsxC"
                     /locus_tag="OO448_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6338108.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10833..11891
                     /gene="rsxD"
                     /locus_tag="OO448_002952"
     CDS             10833..11891
                     /gene="rsxD"
                     /locus_tag="OO448_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6338109.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11895..12515
                     /gene="rsxG"
                     /locus_tag="OO448_002953"
     CDS             11895..12515
                     /gene="rsxG"
                     /locus_tag="OO448_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6338110.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12519..13214
                     /gene="rsxE"
                     /locus_tag="OO448_002954"
     CDS             12519..13214
                     /gene="rsxE"
                     /locus_tag="OO448_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6338111.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13214..13849
                     /gene="nth"
                     /locus_tag="OO448_002955"
     CDS             13214..13849
                     /gene="nth"
                     /locus_tag="OO448_002955"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6338112.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14460..15962
                     /gene="dtpA"
                     /locus_tag="OO448_002956"
     CDS             14460..15962
                     /gene="dtpA"
                     /locus_tag="OO448_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6338113.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16067..>16181
                     /locus_tag="OO448_002957"
     CDS             16067..>16181
                     /locus_tag="OO448_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6338114.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
BASE COUNT         3761 a         4116 c         4125 g         4179 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat tgaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa agccgccgtc gaagccgcta
    10681 ttgcccgtgc caaagcgcgc aagctggaac agcaacagac taacgcagaa ccagaagaac
    10741 aggttgatcc gcgcaaagcg gcagttgccg cggctattgc ccgcgctcag gccaaaaaag
    10801 ccgcccagca gaaggttgta aacgaggact aaatggtatt cagaatagct agctcccctt
    10861 atacccataa ccagcgccag acatcgcgca ttatgctgtt ggtgttgctc gcagccgtgc
    10921 caggaatcgc agcgcaactg tggttttttg gttggggtac tctcgttcag atcctgttgg
    10981 catcggttag tactctgtta gccgaagctc tcgtactcaa actacgcaag cagtcggtag
    11041 ccgcaacgtt gaaagataac tcagcattgc tgacaggctt attgctggcg gtaagtattc
    11101 cccccctcgc gccatggtgg atggtcgtgc tgggtacggt gtttgcggtg atcatcgcta
    11161 aacagttgta tggcggtctg gggcaaaacc cgtttaatcc ggcaatgatt ggttatgtgg
    11221 tcttactgat ctccttcccg gtgcagatga ccagctggtt accgccacat gaaattgcgg
    11281 tcaacatccc tggttttatc gacgccatac aggttatttt cagcgggcat accgccagtg
    11341 gtggtgatat gaacacacta cgcttaggta ttgatggcat tagtcaggcg acaccgctgg
    11401 atacatttaa aacctctgtc cgtgccggtc attcggttga acagattatg caatatccga
    11461 tctacagcgg tattctggcg ggcgctggtt ggcaatgggt aaatctcgcc tggctggctg
    11521 gcggcctgtg gttgctatgg cagaaagcga ttcgctggca tattcccctc agcttcttag
    11581 taacgctggc gttatgcgca acgttgggct ggttgttctc accagaaaca ctggcagcac
    11641 cgcaaattca tctgctgtct ggtgcgacca tgctcggcgc attctttatt ttgactgacc
    11701 cggttaccgc ttctacgacc aatcgtggtc gtcttatttt cggcgcgctt gcgggcttat
    11761 tagtctggtt gatccgcagt ttcggcggct atcctgacgg cgtggctttt gccgtcctgc
    11821 tggcgaacat cacggttcct ctgatcgatt actacacgcg tccgcgcgtc tacggccatc
    11881 gcaaagggta aaccatgctg aaaactatcc gaaaacacgg cattacgtta gcgctatttg
    11941 cagcgggttc aacagggtta actgcggcca tcaaccagat gaccaaaacg acgattgctg
    12001 aacaggccag tctgcaacaa aaggcattat ttgatcaggt gctgccagcc gaacgctata
    12061 acaatacgct ggcacagagt tgctatctgg taactgcgcc agagttaggt aaaggtgagc
    12121 atcgggttta catcgccaaa caggatgaca aaccggtagc cgccgttctg gaagcaaccg
    12181 cgccagatgg ctattccggt gcgattcagc tgctggtggg agccgatttt aacggcacgg
    12241 tacttggcac gcgcgtgaca gagcaccacg aaacgccagg gcttggcgat aaaatcgaac
    12301 tgcgcctttc tgactggatc acccattttg cgggtaaaaa aatcagtggt gcggatgatg
    12361 cgcactgggc ggtgaagaaa gatggtggtg atttcgacca gttcaccggc gcgacgatta
    12421 ctccccgcgc ggtggttaat gcggtaaaac gcgccggatt gtacgctcag acgttaccgg
    12481 cacaactttc tcaacttcct gcctgtggag aataaagcgt gagcgaaatt aaagacgtta
    12541 ttgttcaggg gttgtggaaa aacaactctg cgctggtcca gttgctcggc ctttgtcctc
    12601 tgttggcggt cacgtccact gccactaacg ctctgggttt aggacttgcg actacgctgg
    12661 tactgacgct gaccaacctg accatttcga cgctgcgtca ctggacgcca gccgagatcc
    12721 gcattcccat ttacgtgatg atcatcgcct cggtggtcag cgctgtacag atgctgatca
    12781 acgcctacgc ctttggcctg tatcaatcat tagggatttt tattccgctg attgtcacta
    12841 actgtatcgt tgtgggccgc gctgaagcct tcgccgccaa aaaaggtccg gcgctttcgg
    12901 cactggacgg cttttcaatt ggtatgggcg caacctgcgc catgttcgtg ctgggttcac
    12961 tacgcgaaat tatcggcaat ggcacattgt ttgacggtgc agatgcgctg ttaggtagct
    13021 gggcaaaagt attacgcgtg gagattttcc acaccgactc ccctttcctg ctggcgatgc
    13081 tgccaccagg tgcatttatt ggcctgggac tgatgctggc aggaaaatac ctgattgatg
    13141 aaagaatgaa aaagcgccgt actgaagcag ctgccgaacg tgcattgcca aacggtgaaa
    13201 cagggaatgt ctgatgaaca aagcaaaacg cctggagatc ctcactcgcc tgcgtgagaa
    13261 caatcctcat cccaccaccg agcttaattt cagttcgcct tttgaattgc tgattgccgt
    13321 actgctttcc gctcaggcga ccgatgtcag tgttaataag gcgacggcga aactttaccc
    13381 ggtggcgaat acgcctgcag cgatgcttga actgggcgtt gaaggggtta aaacctatat
    13441 caaaacgatt gggctttata acagcaaagc agaaaatatc atcaaaacct gccgtatctt
    13501 actggagcag cataatggcg aggttccgga agatcgtgct gcgcttgaag ccctgcccgg
    13561 cgtaggtcgt aaaacagcca acgtcgtatt aaacactgca ttcggctggc cgactattgc
    13621 tgtcgacacg cacattttcc gcgtttgtaa tcgtactcaa tttgcgccgg ggaaaaacgt
    13681 cgaacaggta gaagaaaagc tactgaaagt ggttccggca gagtttaaag tcgactgcca
    13741 ccattggttg atcctgcacg ggcgttatac ctgcattgcc cgcaagcccc gctgtggctc
    13801 ttgtattatt gaagatcttt gtgaatacaa agagaaagtt gacatctgaa gaaaaggggt
    13861 aacaccgatt accccattga taacctttct ttatcctctt ttaaaacatc tttttaacgt
    13921 gaatgatgcc attgcttagc gttatcatca ggtaatccgt ttgcggataa ccaaaaatgc
    13981 aggttaattg tttttttaat agcgaaattt tctattcatt cgtgatcaag atcacgatgc
    14041 tgtcgcaatg taaaaacatt gcaagaatca ggttaaaata atttaaattt acacacgcaa
    14101 caaatattga cctacaaaac attacactgg ctatttttca gaaactggac tatctcacta
    14161 gtaaaaacgc taaatagcag aacatatcgc cgttaagcca tcacccccct ctcagtgcag
    14221 tgaaaaaatc tgccattacg ttttttgaaa aatttaacgc tggataacat ttcccggaat
    14281 ggttgaattc cccgcctcag ttatatgtaa cagattatta caaaggactt gtctgaaagt
    14341 gcaagatagt gaacattacc tgccgtttcc cctcccacta taacaattgc gcgtatgttt
    14401 tttagacata acgcgagaaa gcacccccgt taatatggga tgtaaaaaaa gaggtaaaag
    14461 tgtccactgc aaaccaaaaa ccaactgaaa gcgtcagttt gaacgctttc aaacaaccga
    14521 aggcgttcta tctcatcttc tcgattgagt tatgggaacg ttttggttat tacggcctac
    14581 aaggaattat ggctgtttac ctggttaaac aactgggtat gtctgaagcg gattcaatca
    14641 cccttttctc ttcctttagt gccctggttt atggtctggt cgctatcggc ggctggttag
    14701 gtgacaaggt actgggtact aaacgcgtaa ttatgctcgg tgctattgtg ctggcgattg
    14761 gttatgctct ggttgcctgg tctggtcacg acgccggtat cgtttatatg ggtatggcgg
    14821 ctattgcggt cggtaacggc ctgtttaaag ctaacccatc ttctctgctt tctacatgct
    14881 atgagaaaaa cgacccgcgt ctggacggtg cattcaccat gtactacatg tccgtcaaca
    14941 tcggctcttt cttctctatg attgctacgc cgtggctggc cgcgaaatac ggctggagtg
    15001 ttgcgtttgc gttgagcgtt gtaggcctgc tgatcactat cgttaacttc gccttctgcc
    15061 aacgctgggt taaacagtac ggttcaaaac cagacttcga gcctatcaac taccgtaacc
    15121 tgctgctgac cattattggt gttgtggcac tgatcgctat cgccacctgg ctgctgcaca
    15181 atcaggaagt tgcgcgtatg gcgctgggcg ttgttgcctt cggtatcgtg gttatcttcg
    15241 gtaaagaagc cttcgcgatg aaaggtgctg cgcgtcgtaa aatgatcgtt gccttcatcc
    15301 tgatgctcga agccattatc ttcttcgtgc tgtacagcca gatgccaacg tcactgaact
    15361 tctttgcgat tcgtaacgtt gagcactcca ttctgggtct ggccgtagaa cctgagcagt
    15421 atcaggcact gaacccgttc tggatcatca tcggtagtcc gattctggcc gctatctata
    15481 acaagatggg cgataccctg ccgatgccaa ccaagtttgc aatcggcatg gtgatgtgtt
    15541 ctggtgcgtt cctgattctg ccgctgggtg cgaaattcgc gtctgacgct ggtatcgtgt
    15601 ctgtaagctg gctggtcgca agctatggcc tgcagagcat cggggaactg atgatctctg
    15661 gtctgggtct ggcaatggtt gcacaactcg ttccgcagcg tctgatgggc ttcattatgg
    15721 gtagctggtt cctgaccact gccggtgcaa acctgattgg tggttatgtt gcgggtatga
    15781 tggctgtgcc ggataacgtt accgacccgc tgatgtcact ggaagtttat ggtcgcgtat
    15841 tcttgcagat tggtgtcgct actgccatta ttgcagtact gatgctgctg accgcgccta
    15901 aactgcaccg tatgacgcag gatgacgctg cagacaaagc ggcgaaagct gctgtagcgt
    15961 aaaattcagg gaaactcttt tacaagccgc taacttttcg ttagcggctt ttttttgttc
    16021 agcaacgcac taccatactt taaaccacag acaaaaggag ttaccgatga aattgttcta
    16081 caaaccgggt gcctgctctc tcgcttccca tatcaccctg cgtgagagcg gaaaggattt
    16141 taccctcgtc agtgtggatt taatgaaaaa acgtctcgaa a
//
LOCUS       DAJTVJ010000093        15964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000093 DAJTVJ010000000
VERSION     DAJTVJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.093"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="OO448_002958"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="OO448_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6338115.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="OO448_002959"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="OO448_002959"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6338116.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OO448_002960"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OO448_002960"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6338117.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OO448_002961"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OO448_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OO448_002962"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OO448_002962"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6338118.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OO448_002963"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OO448_002963"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR6338119.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="OO448_002964"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="OO448_002964"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6338120.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="OO448_002965"
     CDS             8229..8939
                     /locus_tag="OO448_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6338121.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTPLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="OO448_002966"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="OO448_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6338122.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="OO448_002967"
     CDS             9636..10343
                     /locus_tag="OO448_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6338123.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="OO448_002968"
     CDS             10343..10891
                     /locus_tag="OO448_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6338124.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="OO448_002969"
     CDS             10901..12067
                     /locus_tag="OO448_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6338125.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="OO448_002970"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="OO448_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="OO448_002971"
     CDS             13576..14229
                     /locus_tag="OO448_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6338126.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="OO448_002972"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="OO448_002972"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6338127.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15964)
                     /locus_tag="OO448_002973"
     CDS             complement(15612..>15964)
                     /locus_tag="OO448_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6338128.1"
                     /translation="LFYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAA
                     KHQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTR
                     VWSGYLTLKSLQRQ"
BASE COUNT         3701 a         4346 c         4163 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgcccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 agaa
//
LOCUS       DAJTVJ010000094        15916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000094 DAJTVJ010000000
VERSION     DAJTVJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.094"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..158
                     /locus_tag="OO448_002974"
     CDS             <1..158
                     /locus_tag="OO448_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6338129.1"
                     /translation="DFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFGMTPG
                     EYRRKFR"
     gene            complement(330..659)
                     /locus_tag="OO448_002975"
     CDS             complement(330..659)
                     /locus_tag="OO448_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6338130.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(664..849)
                     /locus_tag="OO448_002976"
     CDS             complement(664..849)
                     /locus_tag="OO448_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6338131.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(846..3485)
                     /locus_tag="OO448_002977"
     CDS             complement(846..3485)
                     /locus_tag="OO448_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6338132.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3693..4682
                     /gene="ldhA"
                     /locus_tag="OO448_002978"
     CDS             3693..4682
                     /gene="ldhA"
                     /locus_tag="OO448_002978"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6338133.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4793..5215
                     /gene="hslJ"
                     /locus_tag="OO448_002979"
     CDS             4793..5215
                     /gene="hslJ"
                     /locus_tag="OO448_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6338134.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5212..5478)
                     /locus_tag="OO448_002980"
     CDS             complement(5212..5478)
                     /locus_tag="OO448_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6338135.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5752..9276
                     /gene="nifJ"
                     /locus_tag="OO448_002981"
     CDS             5752..9276
                     /gene="nifJ"
                     /locus_tag="OO448_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6338136.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9642..10775
                     /gene="ompN"
                     /locus_tag="OO448_002982"
     CDS             9642..10775
                     /gene="ompN"
                     /locus_tag="OO448_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR6338137.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10916..11350
                     /gene="uspF"
                     /locus_tag="OO448_002983"
     CDS             10916..11350
                     /gene="uspF"
                     /locus_tag="OO448_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6338138.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11936..12850
                     /gene="sitA"
                     /locus_tag="OO448_002984"
     CDS             11936..12850
                     /gene="sitA"
                     /locus_tag="OO448_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6338139.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12850..13677
                     /gene="sitB"
                     /locus_tag="OO448_002985"
     CDS             12850..13677
                     /gene="sitB"
                     /locus_tag="OO448_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6338140.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13674..14531
                     /gene="sitC"
                     /locus_tag="OO448_002986"
     CDS             13674..14531
                     /gene="sitC"
                     /locus_tag="OO448_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6338141.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14528..15385
                     /gene="sitD"
                     /locus_tag="OO448_002987"
     CDS             14528..15385
                     /gene="sitD"
                     /locus_tag="OO448_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6338142.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15586..>15916)
                     /locus_tag="OO448_002988"
     CDS             complement(15586..>15916)
                     /locus_tag="OO448_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6338143.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT         3982 a         4017 c         3828 g         4089 t
ORIGIN      
        1 tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg gcaggcatcg
       61 gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag caacgctttg
      121 ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt gtgtgtactg
      181 caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa aaggcaattt
      241 gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc tttctcctgt
      301 agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt accgtttatc
      361 ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc gagtttcgcg
      421 atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc tttacgttcg
      481 gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc aaccagataa
      541 ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt caatgccatt
      601 acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt cttcatcatc
      661 gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt ctgccttgat
      721 gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct tgggcgcagc
      781 gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg cagccagtaa
      841 aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc cggcagacgt
      901 gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg ccacagcgta
      961 aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc atcaacgcga
     1021 ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa gttaattttt
     1081 gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc cgcagtcata
     1141 ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat tggcccgcta
     1201 tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa cggcaatgca
     1261 ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac cagttcgctg
     1321 gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg taattgtttc
     1381 atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa gggttcttct
     1441 tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt caccagatta
     1501 atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca ggctccatcg
     1561 gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg catccaggca
     1621 ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg cgcagcagaa
     1681 aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc gggtggcacc
     1741 agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg cagacgaata
     1801 ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa atcgccttta
     1861 aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga tggcggtaac
     1921 acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc gctgaaggag
     1981 ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg cggcttttct
     2041 accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt cagcgtaatc
     2101 gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat cggtgtaaaa
     2161 ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa atcattcgcc
     2221 agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg caaaatggcc
     2281 tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg ccagcggatt
     2341 tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc gcctggctca
     2401 aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg ggcatataaa
     2461 atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct gttatccatg
     2521 cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg cccctgggtc
     2581 agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca gttgtcgact
     2641 ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca gcgaccatcg
     2701 cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg atcgccgtta
     2761 tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc tgcatccacc
     2821 agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac aggaagtcca
     2881 tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg attttcaaac
     2941 gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc ttgaaattta
     3001 actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag agagagtttt
     3061 ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca ggtgttaggc
     3121 agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc cgtctgcggc
     3181 aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa ccagcggctg
     3241 ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac cagataacgg
     3301 agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa tgcaatacgt
     3361 gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag cgtcatcagc
     3421 agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt atatttaccc
     3481 agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac gggtattgtg
     3541 gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt tgatgaattt
     3601 ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt ttagtagctt
     3661 aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat agcacaaaac
     3721 agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag ctggaatttt
     3781 ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa gcggtatgta
     3841 ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag cacggcgtta
     3901 aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg gcaaaagaac
     3961 tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct gaacacgcca
     4021 tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt acccgtgacg
     4081 ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg gcaggcgtta
     4141 tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt ggtatgcgtc
     4201 tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg gagtatgtcg
     4261 atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg ctgacaccgg
     4321 aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc gtgatgatcg
     4381 tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg ctgaaaaatc
     4441 agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta ttctttgaag
     4501 ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc tgccacaacg
     4561 tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt atttctcaga
     4621 ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac gaactggttt
     4681 aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca aagacctttc
     4741 atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca caatgaagaa
     4801 agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta atgacaaaat
     4861 tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg taaacggtaa
     4921 gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa tgatgatttc
     4981 cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg aactgacagc
     5041 caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg aactcgataa
     5101 caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga accagttaac
     5161 gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga attaatagct
     5221 gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg gtaatgcaca
     5281 catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc cgatcattgc
     5341 acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta cgtgcgccgc
     5401 agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg cacaccctac
     5461 ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac accgccataa
     5521 ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc cgcattttta
     5581 cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac gctgatgtgg
     5641 gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg attactactg
     5701 ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca tatgattact
     5761 attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt tatcgccatc
     5821 taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc cggaaacggc
     5881 ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga agcgggtgct
     5941 atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt tacgtcatcg
     6001 cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact cacaccgttt
     6061 gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt tggcgatcat
     6121 tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc aaacgtccag
     6181 gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg cgtgccattt
     6241 attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt cccgctggcc
     6301 gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg cgcccgggcg
     6361 ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac ttatttccag
     6421 tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga acaggcgatg
     6481 aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta cgggcatccg
     6541 caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga agaagtggtt
     6601 gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct gtaccgcccc
     6661 ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt ggcggtactg
     6721 gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt gatgaccgca
     6781 ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat tggtgggcgc
     6841 tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt tgccgagctc
     6901 aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt aaccaatctg
     6961 tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc cttgttttat
     7021 ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat tatcggtaat
     7081 tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc gggcggcctg
     7141 acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct catttcccag
     7201 gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat ggctgagcgt
     7261 ttaaaacctg gtggcatttt cctgctcaac acgccgtaca gcgcagatga agtgtggtcg
     7321 cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt ctatgtgatt
     7381 aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac cgtcatgcag
     7441 atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc agaattgcag
     7501 ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg caactggcag
     7561 gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt aaatccgcac
     7621 agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa aaccgtaacc
     7681 gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc gccagacggc
     7741 acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga gatccccatc
     7801 tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc acactcagct
     7861 attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag cctgcattcg
     7921 ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt ggctccggaa
     7981 gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg tcagaatcca
     8041 gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga gaaaatcaat
     8101 tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg tattgatatt
     8161 cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc cggttgtggc
     8221 gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt gatcgctaac
     8281 gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta taccaccgat
     8341 gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc cgaatttggc
     8401 cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct gctggatcaa
     8461 tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc tacgccagag
     8521 gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc cgaagcacat
     8581 gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat tggtggtgat
     8641 ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt gacggaaaac
     8701 gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca ggcgtcgaaa
     8761 gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa agcgcgtaaa
     8821 gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat ttctctcggc
     8881 gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc gggaccatcg
     8941 ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact cagccatgac
     9001 cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga tcctcgtcgt
     9061 gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga agcactggaa
     9121 gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc agaagtggca
     9181 gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt cctggcacaa
     9241 atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag gattacccaa
     9301 ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg gaaaatcagt
     9361 ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcata taacccgcgc
     9421 agaataacgt atagaaaatt aaaagtataa tttttatttt gtatgtatta tcttcgatac
     9481 attcaccaga cttattatca tttcagaaga attatttgat taaggtttta cttaaggcgt
     9541 aacaaatgat atttatcggc taactgaact tctcctttag gatgttttca ctccccggaa
     9601 tggggataaa aggcaaataa aataactaaa ggatttattc aatgaaaagc aaagtactgg
     9661 cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa gtttataata
     9721 aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat ttttctgata
     9781 attcagcgaa agatggcgat cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa
     9841 ttaacgatca actcactggc tacggtcaat gggaatacaa tattcaggca aacaacactg
     9901 aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cgggctgaaa tttgcagatt
     9961 acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg
    10021 atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt atgactggtc
    10081 gtgccaacgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta aatggtctga
    10141 acttcgcggt gcagtatcaa ggtaacaacg aaggtgccag taatggtcag gaaggcacca
    10201 acaacggacg tgatgttcgc catgaaaatg gtgacggctg gggtctttcc acaacatatg
    10261 atttaggcat gggctttagc gctggtgcgg catacacctc atctgaccgc accaatgacc
    10321 aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct gggctaaaat
    10381 acgatgctaa caatatttac ctggcaacca tgtattcaga aacgcgtaat atgaccccgt
    10441 ttggcgacag cgattatgct gttgcaaaca aaacccagaa ctttgaagtc actgcacagt
    10501 accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
    10561 tgcacgctgc cgatggtgca gacaacccgg caggtgttga tgataaagat cttgttaaat
    10621 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacttat gttgactata
    10681 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
    10741 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
    10801 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
    10861 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
    10921 cagaacgatt cttgtcccta tcgatatttc cgattcagaa ttaactcaac gcgtgattag
    10981 ccacgttgag gcagaggcaa agattgacga tgcagaggtt catttcctga cggtaatacc
    11041 ttcactgcct tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
    11101 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
    11161 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
    11221 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
    11281 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
    11341 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
    11401 gctgacattt tcccctctaa tccgtaccat acacgccaca gttttatatc agatttctta
    11461 tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
    11521 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaagtaagg
    11581 gcaaaaatca caactatctg acccataagt attttattta tttttcaata tgttaaacat
    11641 tatcagcgac aacaataagc tacgataacc cactcttttt ttgccccttt tttgcccctt
    11701 ctacagcgtt ttgccccatt tttgccatca acaattttcc tcataaagtc atttcaaagc
    11761 ctaatagctg ctctactttt ttcctcaata tcatgtcgcc agcaggaaac aacctaatcc
    11821 ctgttttagg cctcaaagct gttaatgata atcattttca ttttgtatgg gttatgctat
    11881 aaaacatagc cgttgctatg tttgttctaa ttttgctcac tataggtact aaattatgca
    11941 ctcaataaaa aaagtaacca tgctcttggg gtgcctcgcc ctcacctgct cgatcgcatt
    12001 tcaggcaagt gcaactgaaa aattcaaggt cattacaaca tttaccatca tcgcggatat
    12061 ggccaaaaac gtggctggag atgctgcaga agtctcatcc ataaccaagc ctggtgcaga
    12121 aattcatgag tatcagccta cccctggcga tattaaacgt gcgcaggggg cacaactgat
    12181 tctcgccaat ggtatgaatc tggaattgtg gttccaacgc ttttaccagc atctcaatgg
    12241 ggttccagaa gtaattgtct cttcgggggt gacgccagta gggatcaccg aaggacccta
    12301 tgagggcaaa cctaatcccc atgcatggat gtcgccagat aatgctctga tttacgtcga
    12361 taatattcgt gatgcgttga taaaatacga tccggcaaat gcacaaacct accaacgtaa
    12421 tgccgatact tataaagcca agattaccca aacccttgcc cccctgcgta agcagattac
    12481 ggaactccct gagaatcagc gatggatggt caccagtgaa ggggcttttt cttatctcgc
    12541 acgcgatttg gggctaaaag agctttatct gtggccgatt aatgccgatc aacaaggaac
    12601 accgcagcag gtacgtaagg ttgttgatat agttaagaaa aatcatatcc cggcagtctt
    12661 tagcgagagt acgatttccg ataaaccagc gcgtcaggtt gcgcgtgaaa ccggcgcaca
    12721 ctacggcggt gtgctctatg tcgattccct gagcacagaa aacggcccgg tacctacgta
    12781 tatcgacctt cttaaggtta ctaccagtac gctggtacag ggaattaaag ccgggaaaag
    12841 ggagaaataa tgatgcaatc tgcaggcatt gtcgttaatg atgttactgt cacctggcgt
    12901 aacgggcaca cagcactgcg cgatgcatcc ttcacagtac caagtggatc tattgccgct
    12961 ctggttgggg taaacggttc cggaaaatcg acgttgttta aagcgattat gggatttgtg
    13021 cgtctgacca gcggaaagat atctgttctg ggtattccca cgcgacaggc gctacagaaa
    13081 aacctggttg cctacgtccc tcagtcagaa gaggttgact ggtcatttcc tgtactggtg
    13141 gaagatgtgg tgatgatggg ccgctatggg catatgggta tgttgcgtat agccaaaaag
    13201 agggatcgtc agattgtcac cgatgcgctg gaacgtgtcg atatggtgga ttttcgccat
    13261 cgtcaaatcg gcgagctttc cggtggacaa aagaaacgcg tctttctggc gagagcgatt
    13321 gcacagcagg gtgatgtgat cctgctggat gaacctttta ccggcgttga tgtgaaaacc
    13381 gaagcgaaaa tcatcagcct gttgagggag ttacgcgcgg aaggcaaaac gatgcttgtc
    13441 tcaacgcaca atctcggctc agtcacgaca ttttgcgatt atacggtcat ggtcaaaggc
    13501 accgtcctgg caagcgggcc gacagacacc acttttacag ccgagaacct ggagctggct
    13561 tttagcggcg tactgcgcca tgtcacactt aacgactcgg aagaaagcat tattactgac
    13621 gatgaacgcc cttttgtggc acatcgaccg tcagcggtgc agagggaagc aagatgaacg
    13681 tgctactgga acccttcagc tatgaatata tgctcaatgc gatgtgggtc tcggcaatgg
    13741 tcggcggtct ctgcgctttc ctgtcatgct atctgatgct aaaaggctgg tcgctgattg
    13801 gcgatgcgct gtcgcactct attgtcccgg gtgttgcggg ggcatatatg ctggggcttc
    13861 cgttttctct gggagccttc ttttccggcg gactggcagc gggcagcatg ctgtttctta
    13921 accaacgcac ccgcttaaaa gaagatgcca tcatcggcct gatcttctcg tccttttttg
    13981 gcctggggct gtttatggtg tcattgaacc cgacatccgt gaacattcag accatcgtcc
    14041 tgggcaatat tctggcaatc gatccggcgg atatcctgca actcactatc attggcattc
    14101 tctcgatcat cgtgcttttt ttcaaatgga aagatcttat ggtgaccttt ttcgacgaga
    14161 atcacgcccg tgctattgga ttacatccgg gcagattaaa aatcctcttc ttcaccctgc
    14221 tgtcggtttc gaccgttgcg gcgctgcaaa cggttggtgc ctttctggtg atctgtctgg
    14281 tggtgactcc cggtgcaaca gcatggttgt taaccgatcg ttttccccgc ctgctcataa
    14341 ttgctgtaac tattggcagt gtaaccagtt tcctcggcgc gtgggtcagt tactttctgg
    14401 acggcgcgac aggtggaatc atcgtggtgg cgcagacact gctgtttctt ttagcatttg
    14461 tatttgcgcc cacccatgga cttttggcaa atcgtcggcg tgcgcataaa gcgctggagg
    14521 atcgctcatg atggcattat tactggagcc actgcagttc acctttatga gtcacgcact
    14581 gctgatttca ctggtagttt ccataccctg tgcgctgctg tcggtctttc tggtcctgaa
    14641 aggttgggca ctgatgggtg acgcgatgag tcatgcggtt tttcctggca tcgtgcttac
    14701 ctggattctg gggttacctc tggctacagg ggcattcgtc gccggagtat tctgcgcggt
    14761 ggcgacggga tacctgaaag acaacagccg aatcaagcaa gatacagtta tggggattgt
    14821 tttttctggc atgtttgccg caggtctgat cttgtatatt gcaatcaaac cagacgtaca
    14881 tcttgaccat attcttttcg gcgacatgct cgggataacc atcggcgata taatccagac
    14941 ggtgattatt gccgggctgg ttacacttgt tattagcgca aaatggcggg attttttgct
    15001 gttcagcttt gattatcaac aggcgcaggt aagcggtttg catacgagat ggctgcatta
    15061 tgggctgctg tgcatggttt ccctgaccat tgtggcgacg ctgaaagcag taggtatcat
    15121 tctctctatt tctctgctca ttgcgcctgg cgccattgca gtactcctga cacaacgctt
    15181 tcatattgcg ctcctactgg cgactggcat atcggtaata gtgtcaatga ctggtgtctg
    15241 gctgtccttt tttatcgaca gcgcaccagc tccgacgatt gtcgtcttat tcgcggtcgt
    15301 gttcatcatg acgtttaccg ttaccagcat caacgcacgc acaaagggaa acgcccatac
    15361 acaggatctg ttatcaccca attagcacaa catccgagga cagcatcgtc cgccagccta
    15421 cagaagtccg gaaagccatg gaaggtcgtt gggaggaggt acgaattcta tcatgcaaaa
    15481 aatacgtaaa atcgataacg cctagaaatc attcaatact cgcactatcg aaagtttacc
    15541 agccaaccgc ggcacgttcc tgcatacgac gtgccgcagc ttctgttatt ccacgataaa
    15601 attcctaacc gaattcaaat ccttcagact attcagctct tccttcagct cccgctgacg
    15661 gcgataaatc tcgtcattgc gctcaacctg tgcctgagcc attgctgccg ccagttcttc
    15721 cagttccggc atcgactgtt tcacctgctg attatcggca tcaccccacg tcatcacgtc
    15781 ccgtacaatg tcggatttcg cagccattac caccggataa aggcggccca gtgagttggg
    15841 gccagcattc cagatacgac cgttccattc aaacgtgaac ggcttcgcct cctgttctgt
    15901 gcgccatgct tcaatt
//
LOCUS       DAJTVJ010000095        15863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000095 DAJTVJ010000000
VERSION     DAJTVJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.095"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..566)
                     /locus_tag="OO448_002989"
                     /pseudo
     CDS             complement(1..566)
                     /locus_tag="OO448_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(800..2220)
                     /gene="espX1"
                     /locus_tag="OO448_002990"
                     /pseudo
     CDS             complement(800..2220)
                     /gene="espX1"
                     /locus_tag="OO448_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2705..2968)
                     /gene="rpsT"
                     /locus_tag="OO448_002991"
     CDS             complement(2705..2968)
                     /gene="rpsT"
                     /locus_tag="OO448_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6338144.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3071..3289
                     /locus_tag="OO448_002992"
     CDS             3071..3289
                     /locus_tag="OO448_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6338145.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3297..4238
                     /gene="ribF"
                     /locus_tag="OO448_002993"
     CDS             3297..4238
                     /gene="ribF"
                     /locus_tag="OO448_002993"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6338146.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4281..7097
                     /gene="ileS"
                     /locus_tag="OO448_002994"
     CDS             4281..7097
                     /gene="ileS"
                     /locus_tag="OO448_002994"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6338147.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7097..7591
                     /gene="lspA"
                     /locus_tag="OO448_002995"
     CDS             7097..7591
                     /gene="lspA"
                     /locus_tag="OO448_002995"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6338148.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7716..8165
                     /gene="fkpB"
                     /locus_tag="OO448_002996"
     CDS             7716..8165
                     /gene="fkpB"
                     /locus_tag="OO448_002996"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6338149.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8167..9117
                     /gene="ispH"
                     /locus_tag="OO448_002997"
                     /gene_synonym="lytB"
     CDS             8167..9117
                     /gene="ispH"
                     /locus_tag="OO448_002997"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6338150.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9183..10097
                     /gene="rihC"
                     /locus_tag="OO448_002998"
     CDS             9183..10097
                     /gene="rihC"
                     /locus_tag="OO448_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6338151.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10264..11085
                     /gene="dapB"
                     /locus_tag="OO448_002999"
     CDS             10264..11085
                     /gene="dapB"
                     /locus_tag="OO448_002999"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6338152.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11541..12689
                     /gene="carA"
                     /locus_tag="OO448_003000"
     CDS             11541..12689
                     /gene="carA"
                     /locus_tag="OO448_003000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6338153.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12707..>15863
                     /gene="carB"
                     /locus_tag="OO448_003001"
     CDS             12707..>15863
                     /gene="carB"
                     /locus_tag="OO448_003001"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6338154.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAM"
BASE COUNT         3861 a         3743 c         4207 g         4052 t
ORIGIN      
        1 tcatcccccc agtagaaagg aatggagata aacagattaa aacgctcctc ttctttatta
       61 ttttcatcat aagattggct ggcagagaaa gtataactaa tgtgttgcca gttattggag
      121 taactgaatt ggtaatcttt agcatttcca cttcgccccc agtaattccg ccaaagcgca
      181 cttaatgata catttcccag attattggat aaaggttgca taatattggc agaaaggcta
      241 ttttttctgc caatatcata aaaatcatca taatcactct gatgatagtg tttatcattt
      301 tcgtaaagat ggtcgctgaa tgtcctgtaa tcctgcgaag cataacgcca ggcggccacg
      361 ctaaatcgag tggctgtttg tactagatat ttattatagg cgacctgata gctcgtacct
      421 tcgtgtctgg tatcattatt taacttacta ctcgatcggg cagcgtaaaa agagatggca
      481 cccagcggtg tattccaacc atttcccaga gttatagcat tatagttatc ggataaaatc
      541 gtacccgatt atgtgatcag gttgttatta tttagattaa tgtttaaatt ttttaatttc
      601 ttgatgtgaa gttgatgaaa gtttgaaaat ttaatattct taattgttcc cttctttata
      661 agaagagcaa ggtgccatca ttagagtgac agagtaggat gtttcagagc atgcttcaga
      721 ttgtttttta tttctctgca gtgtattaat aatcatcaca gataatttca ttctaaaatt
      781 acctctttct ttgagtgttt taatcctgat gaactatatt ttttataagg agcatgcact
      841 cgttgatgct ctgaaggtat tttctcattt ttgatgatgt tttgtcgtaa tcgtgcataa
      901 acttattaag ttctttatac acatcattat ttagctttac attatcgcga actaaagttt
      961 gtaccaatgg tatcaaaatt tcgaaacggg acggtttgtc atcaatactt gccagggttt
     1021 tgaatactcc tgtgtatctg taacctaagt gcttattgac aagttgagtg gtccatcggc
     1081 caggcataat atcttcacag cgcaaatttt ttatcatggt attagtgaac gatgttccaa
     1141 atatgttgct ttttttaatg aagaggttgt ttatttctcc atttgaaaag atggtgtttg
     1201 ataagtcgct ttcaaaacat gatatattgg ttaatacaca acaaaaataa tacccatatg
     1261 aaaggttgct cttttctatt gttatatttg atagcaacat tttatgtata ttaatattaa
     1321 gagcagtcaa atactggatc ttttgatcgt taagttcata catctccagt gatttattat
     1381 agggtaataa aagtataaat tcctttatat tatctccgca gtaagaaaag acgacattaa
     1441 atttattagt ggccatgttt tcagttgagc agtaagctga tttttctatt aaccgactgg
     1501 gcacgctttt aaataaagat aattttccac atagttttga atatgccgaa ttgctagttt
     1561 taaagttctg ccacattatt ccctggttat catctatcga tgaggagggg attttattgc
     1621 attgctcatt gtatagctca gggaaaatat tttttttaat aagctttttg atttcctggt
     1681 taagatgact ttttttcttg tctattgcac tgtattttgc gttttgtgac gtttcctgat
     1741 gatcataaaa tgactggaga tttctgcagg cctcataaaa tatttcagga gtatatagct
     1801 gtgggtatat ttcttggttg aagtttgcct ttaaaaaatt aacgaatttt tcatggcatt
     1861 cttggtatat cttaatctct tcagaaagac attcgcttgt tctgtcatca aaaacatcca
     1921 atctaagtaa tgtgatacct ttgtgtttaa aaaataaaat tgaactgtat gttctatgag
     1981 tttacatcta taaaaatatg catctctcag atgagtatat tgaaaaaaat gatgaaggac
     2041 tggagatggt ttaggcaagt tatggcttct aaaaaagtca tcaaacaaaa ttaagaactc
     2101 tgttttatac tcgcaaaata cttttactaa ctcattttgt cttgttttat ttattattac
     2161 atcatctact atatttgatt tataaagaca atgtggcgaa gttgagatac catttgtcat
     2221 tatcagtcct tgaagttgat gaatttttat taatttaatt tatgagtgaa tgtgactact
     2281 atcaaaaata gctcattaat tttatgaagt agaatataaa taggtattta acctgtgttg
     2341 attgttgtta attatttaca agatctaata aggagatatt tatgaatggt taaagtgtat
     2401 aggaatttaa ttcaaaaata gagtggtctt aagtttaata cctcaatgtg tatcacaata
     2461 tccacattct ttgtagggga gtctggagat tgagtagata ttcttgttca gaatgtatca
     2521 gccgatggtt ctacgattct taagccacga agagttcaga aagtgtaacg gcatgtctgc
     2581 aaaagccagc atcttttgac tatctggcaa ccggtagtgt gttctctcac gcatcataaa
     2641 agcagcaggc ataaaaaaaa cccgcttgcg cgggcttttt cacaaagctt cagcaaattg
     2701 gcgattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt gcagctttgt
     2761 ttttgtggat cagaccttta gcagcctgac ggtccacgat cggttgcatt tcgttaaatg
     2821 ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc ttgatgaaag
     2881 tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac tgaatggcgc
     2941 gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc tatatggaca
     3001 attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt tgtgcaaata
     3061 agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct accagcttgc
     3121 ggggtgtgaa tacagctttt ccgcgataaa aattgcagca ggcggtcact ttcttcccgt
     3181 gatttgcgcc atggcaatga aaagccactt ctttctgatt tcggtactca atcgccggtt
     3241 aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag ccagacatga
     3301 agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt gtgctgacta
     3361 ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc ttgcaggaag
     3421 aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa ccactggaac
     3481 tgtttgctac cgataaagcc ccggcaagac tgacccggct gcgggaaaaa ctgcgttacc
     3541 ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt
     3601 taaccgcgca aaatttcatc agcgatcttc tggtgaagca tttgcgcgta aaatttcttg
     3661 ccgtagggga tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg ttattacaga
     3721 aagctagcat ggaatacggc ttcgatatca ctagtacgca aactttttgc gaaggtggcg
     3781 tgcgtattag cagcaccgct gtgcgtcagg cgctggcgga tgacaatctg gctctggcag
     3841 aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt gatgaattag
     3901 ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt tccccggtga
     3961 aaggggttta tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa
     4021 acatcggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg catttgttag
     4081 atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca
     4141 atgagcagcg atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa
     4201 ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta atcaaaccga
     4261 aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg ccggaaacag
     4321 ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg cgttggactg
     4381 atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc
     4441 atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt aacaagattc
     4501 tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg tatgtgcctg
     4561 gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg
     4621 gtgagaaatt caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg
     4681 ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt
     4741 acctgaccat ggacttcaaa actgaagcca acatcatccg cgcgctgggc aaaatcatcg
     4801 gcaacggtca cctgcacaaa ggcgcgaagc cagttcactg gtgcgttgac tgccgttctg
     4861 cgctggcgga agcggaagtt gagtattacg acaaaacttc tccgtccatc gacgttgctt
     4921 ttcaggcggt cgatcaggat gcactgaaag caaaatttgc cgtaagcaac gttaacggcc
     4981 caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac cgcgcaatct
     5041 ctattgcacc tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg
     5101 cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac accattctcg
     5161 gcacggtaaa aggtgcggag cttgagttgc tgcgctttac ccatccgttt atgggcttcg
     5221 acgttccggc aatcctcggc gatcacgtta ccctagatgc cggtaccggt gccgttcaca
     5281 ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc ctggaaaccg
     5341 ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg ctggatggcg
     5401 tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc
     5461 tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa acgccgatca
     5521 tcttccgcgc gacgccgcag tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt
     5581 cactgaaaga gatcaaaggc gtgcagtgga tcccggactg gggccaggcg cgtatcgagt
     5641 cgatggttgc taaccgtcct gactggtgta tctcccgtca gcgcacctgg ggcgtaccga
     5701 tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtactctc gaactaatgg
     5761 aagaagtggc aaaacgcgtt gaagttgacg gcatccaggc gtggtgggat cttgatgcga
     5821 aagagatcct cggcgatgaa gctgatcagt acgtgaaagt gccggacaca ttggatgtat
     5881 ggtttgactc cggatctacc cactcttctg ttgttgacgt gcgtccggaa tttgccggtc
     5941 acgcagcgga catgtatctg gaaggttctg accagcaccg tggttggttc atgtcttccc
     6001 taatgatctc caccgcgatg aaaggcaaag cgccgtatcg tcaggtactg acccacggct
     6061 ttaccgtgga tggtcagggt cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc
     6121 aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca tcaaccgact
     6181 acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgctgccgat agctatcgtc
     6241 gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat ccagcaaaag
     6301 atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag
     6361 cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa gtggtacagc
     6421 gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc atcaaagacc
     6481 gtcagtacac cgccaaagcg gacagtgtgg cgcgtcgtag ctgccagact gcgctgtatc
     6541 acatcgcaga agcgctggtg cgctggatgg caccaatcct ctccttcacc gctgatgaag
     6601 tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggtgag tggtacgaag
     6661 gcctgtttgg cctggcagac agtgaagcga tgaacgatgc gttctgggac gagctgttga
     6721 aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg
     6781 gctcgctgga agcggcagta accttgtatg cagaaccgga actgtcggcg aaactgaccg
     6841 cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt gcagactata
     6901 acgacgcacc tgctgatgct cagcagagcg aagtactcaa agggctgaaa gtcgcgttga
     6961 gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat gtcggcaagg
     7021 tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa
     7081 aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct ggctgtggct
     7141 ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc agaactttgc
     7201 tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc gtaactatgg
     7261 cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct ttgccggtat
     7321 tgcgattggt attagcgtga tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa
     7381 gctaaacaat atcgcttacg cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg
     7441 cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact ggcacttcgc
     7501 caccttcaac cttgccgata ctgccatctg tgtcggtgcg gcactgattg tgctggaagg
     7561 ttttttgcct tctagagcga aaaaacaata ataaaccctg ccggatgcga tgctgacgca
     7621 tcttatccgg cctacagatt gctgcgaaat cgtaggccgg ataaggcgtt tacgccgcat
     7681 ccggcaaaaa tcctcaaata taagagcaaa cctgcatgtc tgaatctgta cagagcaata
     7741 gcgccgtcct ggtgcacttc acgctaaaac tcgacgatgg caccaccgcc gagtctaccc
     7801 gcaacaacgg taaaccggcg ctgttccgcc tgggtgatgc ttctctttct gaagggctgg
     7861 agcaacacct gttggggctg aaagtgggcg ataaaaccac cttctcgttg gagccagatg
     7921 cggcgtttgg cgtgccgtca ccggacctga ttcagtactt ttcccgccgt gaatttatgg
     7981 atgcaggcga gccagaaatt ggcgcaatca tgctttttac cgcaatggat ggcagtgaga
     8041 tgcctggcgt gatccgcgaa attaacggcg actccattac cgttgatttc aaccatccgc
     8101 tggccgggca gaccgttcat tttgatattg aagtgctgga aatcgatccg gcattggagg
     8161 cgtaacatgc agatcctgtt ggccaacccg cgtggttttt gtgccggggt agaccgcgct
     8221 atcagcattg ttgaaaacgc gctggccatt tacggcgcac cgatatatgt ccgtcacgaa
     8281 gtggtacata accgctatgt ggtcgatagc ttgcgtgagc gtggggctat ctttattgag
     8341 cagattagcg aagtaccgga cggcgcgatc ctgattttct ccgcacacgg tgtttctcag
     8401 gcggtacgta acgaagcaaa aagccgcgat ttgacggtgt ttgatgccac ctgtccgctg
     8461 gtgaccaaag tgcatatgga agtcgcccgc gccagccgtc gtggcgaaga gtcgattctc
     8521 atcggccacg ccgggcatcc ggaagtggaa ggtacaatgg gccagtacag taacccggaa
     8581 gggggaatgt atctggtcga atcgccggac gatgtgtgga aactgacggt caaaaacgaa
     8641 gagaagctct cctttatgac ccagaccacg ctgtcggtgg atgacacgtc tgatgtgatc
     8701 gacgcgctgc gtaaacgctt cccgaaaatt gtcggtccgc gcaaagatga catctgctac
     8761 gccacgacta accgtcagga agcggtacgc gccctggcag aacaggcgga agttgtgttg
     8821 gtggtcggtt cgaaaaactc ctccaactcc aaccgtctgg cggagctggc ccagcgtatg
     8881 ggcaaacgcg cgtttttgat tgacgatgcg aaagatatcc aggaagagtg ggtgaaagag
     8941 gttaaatgcg tcggcgtgac tgcgggcgca tcggctccgg atattctggt gcaaaatgtg
     9001 gtggcacgtt tgcagcagct gggcggtggt gaagccattc cgctggaagg ccgtgaagaa
     9061 aacattgttt tcgaagtgcc gaaagagctg cgtgtcgata ttcgtgaagt cgattaagtc
     9121 attagcagcc taagttatgc gaaaatgccg gtcttgttac cggcattttt tatggagaaa
     9181 acatgcgttt acctatcttc ctcgatactg accccggcat tgacgatgcc gtcgccattg
     9241 ccgccgcgat ttttgccccc gaactcgacc tgcaactgat gaccaccgtc gcgggtaatg
     9301 tctcggttga gaaaaccacc cgcaacgccc tgcaactgct gcatttctgg aatgcggaga
     9361 ttccgctcgc ccagggagcc gctgtgccgc tggtacgcgc accgcgtgat gcagcatctg
     9421 tgcacggcga atcgggaatg gctggctacg actttgttga gcacaaccga aagccaatcg
     9481 ggataccagc gtttctggcg attcgtgatg ccctgatgcg tgcaccagag cctgttaccc
     9541 tggtggccat tggcccgtta accaatattg cgctgttact ttcacaatgc ccggaatgta
     9601 aaccgtatat tcgtcgtctg gtgatcatgg gcggttcttc tggtcgcggc aactgtacac
     9661 caaatgccga gtttaatatc gctgccgatc cagaagcggc tgcctgtgtc ttccgtagcg
     9721 gaatcgaaat cgtcatgtgc ggtctggatg tcaccaatca ggcaatatta acccctgact
     9781 atctcgctac tttgccggag ttgaatcgca ccggaaaaat gcttcatgca ctgtttagcc
     9841 actaccgtag cggcagtatg caaagcggtc tgcgaatgca cgatctctgc gccatcgcct
     9901 ggctggtgcg cccggactta ttcaccctca agccgtgttt tgtcgcggta gaaactcaag
     9961 gcgaatttac ctcaggcacg acggtggttg atatcgacgg ttgcctgggc aaaccagcca
    10021 atgtaaaggt ggcattggat ctggatgtga aaggcttcca gcagtgggtg gctgaggtgc
    10081 tggctctggc gtcgtaacct gtcacatgtt atcggcatgc agtcattcat cgactcatgc
    10141 ctttcactga tatccctccc tgtttatcat taatttctaa ttatcagcgt ttttggctgg
    10201 cggcgtagcg atgcgctggt tactctgaaa acggtctatg caaattaaca aaagagaata
    10261 gctatgcatg atgcaaacat ccgcgttgcc atcgcgggag ccggggggcg tatgggccgc
    10321 cagttgattc aggcggcgct ggcattagaa ggcgtgcagc tgggcgcggc gctggagcgc
    10381 gaaggatctt ctttactggg cagcgacgcc ggtgagctgg ctggtgcagg gaaaacaggc
    10441 gttaccgtgc aaagcagcct cgatgcggta aaagatgatt ttgatgtgtt tatcgatttt
    10501 acccgtccgg aaggtacgct gaaccatctc gctttttgtc gccagcatgg caaagggatg
    10561 gtgatcggca ctacggggtt tgacgaagcc ggtaaacagg cgattcgtga cgccgccgcc
    10621 gatattgcga ttgtctttgc cgccaatttt agcgttggcg ttaacgtcat gcttaagctg
    10681 ctggagaaag cagccaaagt gatgggtgac tacaccgata tcgaaattat tgaagcacat
    10741 catagacata aagttgatgc gccgtcaggc accgcactgg caatgggaga ggcgatcgcc
    10801 cacgcccttg ataaagatct gaaagattgc gcggtctaca gtcgtgaagg ccacaccggt
    10861 gaacgtgtgc ctggcaccat tggttttgcc accgtgcgtg caggtgacat cgttggtgaa
    10921 cataccgcga tgtttgccga tattggcgag cgtctggaga tcacccataa ggcgtccagc
    10981 cgtatgacat ttgctaacgg cgcggtaaga tcggctttgt ggttgagtgg taaggaaaat
    11041 ggtctttttg atatgcgaga tgtacttaat ctcaataatt tgtaaccaca aaatatttgt
    11101 tatggtgcaa aaataacaca tttaatttat tgattataaa gggctttaat ttttggccct
    11161 tttattttta gtgttatgtt tttaaatatt ctataagtgc caaaaattac atgttttgtc
    11221 ttctattttt gttgttttaa tgtaaatttt gaccatttgg tccacttttt cctgctcgtt
    11281 tttatttcat gcaatcttct tgctgcgcaa gcgttttcca gaacaggtta tatgatcttt
    11341 ttgtcgctta atgcctgtaa aatatgcatg agccacaaaa taatataaaa aataccgcca
    11401 ttaagttgac ttttagcgcc catatctcca gaatgccgcc gtttgccaga aattcgtcgg
    11461 taagcagatt tgcattgatt tacgtcatca ttgtgaatta atatgcaaat aaagtgagtg
    11521 aatattctct ggagggtgtt ttgattaagt cagcgctatt ggttctggaa gacggaaccc
    11581 agtttcacgg tcgggccata ggggcaacag gttcggcggt tggggaagtc gttttcaata
    11641 cttcaatgac cggttatcaa gaaatcctca ctgatccttc ctattctcgt caaatcgtta
    11701 ctcttactta tccccatatt ggcaatgtcg gcactaatga cgccgatgaa gaatcttctc
    11761 aggtacatgc acaaggtctg gtgattcgcg atctgccgct gattgccagc aacttccgta
    11821 ataccgaaga cctctcttct tacctgaagc gccataacat cgtggcgatt gccgatatcg
    11881 atacccgtaa gctgacgcgt ttactgcgcg agaaaggcgc acagaatggc tgcattatcg
    11941 caggcgataa cccggatgcg gcgttggcgt tagaaaaagc ccgcgcgttc ccaggtctga
    12001 atggcatgga tctggcaaaa gaagtgacca ccgcagaagc ctatagctgg acacaaggga
    12061 gctggacgtt gaccggcggc ctgccagaag cgaaaaaaga agacgagctg ccgttccacg
    12121 tcgtggctta tgattttggt gccaagcgca acatcctgcg gatgctggtg gatagaggct
    12181 gtcgcctgac catcgttccg gcgcaaactt ctgcggaaga tgtgctgaaa atgaatccag
    12241 acggcatctt cctctccaac ggtcctggcg acccggcccc gtgcgattac gccattaccg
    12301 ccatccagaa attcctcgaa accgatattc cggtattcgg catctgcctc ggtcatcagc
    12361 tgctggcgct ggcgagcggt gcgaagactg tcaaaatgaa atttggtcac cacggcggca
    12421 accatccggt taaagatgtg gagaaaaacg tggtgatgat caccgcccag aaccacggtt
    12481 ttgcggtgga tgaagcaaca ttacctgcaa acctgcgtgt cacgcataaa tccctgttcg
    12541 acggtacgtt acagggcatt catcgcactg ataaaccggc gttcagcttc cagggtcacc
    12601 cggaagccag ccctgggcca cacgacgcag cgccgttgtt cgaccacttt atcgagttaa
    12661 ttgagcagta ccgtaaaacc gctaagtaat caggagtaaa agagccatgc caaaacgtac
    12721 agatataaaa agtatcctga ttctgggtgc gggcccgatt gttatcggtc aggcgtgtga
    12781 gtttgactac tctggcgcgc aagcatgtaa agccctgcgc gaagagggtt accgcgtcat
    12841 tctggtgaac tccaacccgg cgaccatcat gaccgacccg gaaatggccg atgcgaccta
    12901 catcgagccg attcactggg aagtagtacg caagattatt gaaaaagagc gcccggacgc
    12961 ggtgctgcca acgatgggcg gtcagacggc gctgaactgc gcgctggagc tggagcgtca
    13021 gggcgtgttg gaagagttcg gcgtgactat gattggtgcg accgccgatg cgattgataa
    13081 agcagaagac cgccgtcgtt tcgacgtagc gatgaagaaa attggtctgg aaaccgcgcg
    13141 ttccggtatc gcacacacga tggaagaagc gctggcggtt gccgctgacg tgggcttccc
    13201 gtgcattatt cgcccatcct ttaccatggg cggtagcggc ggcggtatcg cttataaccg
    13261 cgaagagttt gaagaaattt gcgcccgcgg tctggatctc tccccaacca aagagctgct
    13321 gattgatgag tcgctgatcg gctggaaaga gtacgagatg gaagtggtgc gtgataaaaa
    13381 cgacaactgc atcatcgtct gctctatcga aaactttgat gcgatgggca tccataccgg
    13441 tgactccatc actgtcgcgc cagcccaaac gctgaccgac aaagaatatc aaatcatgcg
    13501 taacgcctcg atggcggtgc tgcgtgaaat cggcgttgaa accggtggtt ccaatgtcca
    13561 gtttgcggtg aacccgaaaa acggtcgcct gattgttatc gaaatgaacc cacgcgtgtc
    13621 ccgttcttcg gcgctggcgt cgaaagcgac cggtttcccg attgctaaag tggcggcgaa
    13681 actggcggtg ggttacaccc tcgacgaact gatgaacgac atcactggcg gacgtactcc
    13741 ggcctccttc gagccgtcca tcgactatgt ggttactaaa attcctcgct tcaacttcga
    13801 aaaattcgct ggtgctaacg accgtctgac cactcagatg aaatcggttg gcgaagtgat
    13861 ggcgattggt cgcacgcagc aggaatccct gcaaaaagcg ctgcgcggcc tggaagtcgg
    13921 tgcgactgga ttcgacccga aagtgagcct cgatgacccg gaagcgttaa ccaaaatccg
    13981 tcgcgaactg aaagatgctg gcgcagagcg tatctggtac atcgccgatg ccttccgtgc
    14041 gggcctgtct gtggacggcg tgttcaacct gaccaatatt gaccgctggt tcctggtaca
    14101 gattgaagaa ctggtgcgtc tggaagagaa agtagcggaa gtgggcatca ctggcctgaa
    14161 cgctgaattc ctgcgccagc tgaaacgcaa aggctttgcc gatgcgcgtc tggcaaaact
    14221 ggcgggcgtg cgcgaagcgg aaatccgcaa gctgcgcgac cagtatgacc tgcacccggt
    14281 ttataagcgc gtggatacct gtgcggcaga gttcgccacc gacaccgctt acatgtactc
    14341 cacttatgaa gaagagtgcg aagcgaatcc gtctaccgac cgtgaaaaaa tcatggttct
    14401 cggcggcggc ccgaaccgta tcggtcaggg tatcgaattc gactactgct gtgtacacgc
    14461 ctcgctggcg ctgcgcgaag acggttacga aaccattatg gttaactgta acccggaaac
    14521 cgtctccacc gactacgaca cttccgatcg cctctacttc gagccggtaa ctctggaaga
    14581 tgtgctggaa atcgtgcgta ttgagaagcc gaaaggcgtt atcgttcagt acggcggtca
    14641 gaccccgctg aaactggcgc gcgcgctgga agctgctggc gtaccggtta tcggcaccag
    14701 cccggatgct atcgaccgtg cagaagaccg tgaacgcttc cagcatgcgg ttgagcgtct
    14761 gaaactgaaa caaccggcga acgccaccgt taccgctatt gaaatggcgg tagagaaggc
    14821 gaaagagatt ggctacccac tggtggtacg tccgtcttac gttctcggcg gtcgggcgat
    14881 ggaaatcgtc tatgacgaag ctgacctgcg tcgctacttc cagacggcgg tcagcgtgtc
    14941 taacgatgcg ccagtgttgc tggatcactt ccttgatgac gcggtagaag ttgacgtgga
    15001 tgccatctgc gacggcgaaa tggtgctgat tggcggcatc atggagcaca tcgagcaggc
    15061 gggcgtgcac tccggtgact ccgcatgttc tctgccagcc tacaccttaa gtcaggaaat
    15121 tcaggatgtg atgcgccagc aggtgcagaa actggccttc gaattgcagg tgcgcggtct
    15181 gatgaacgtg cagtttgcgg tgaaaaacaa cgaagtctac ctgattgaag ttaacccgcg
    15241 tgcggcgcgt accgttccgt tcgtctccaa agccaccggc gtaccgctgg caaaagtggc
    15301 ggcgcgtgtg atggctggca aatcgctggc tgagcagggc gtaaccaaag aagttatccc
    15361 gccgtactac tcggtgaaag aagtggtgct gccgttcaat aaattcccgg gtgttgaccc
    15421 gctgttaggg ccagaaatgc gctccaccgg ggaagtgatg ggcgtgggcc gcaccttcgc
    15481 tgaagcgttt gccaaagcgc agctgggcag caactccacc atgaagaaac acggtcgtgc
    15541 gttgctttcc gtgcgcgaag gcgataaaga acgcgtggtg gacctggcgg caaaactgct
    15601 gaaacagggc ttcgagctgg atgcgaccca cggcacggcg attgtgctgg gcgaagcggg
    15661 cattaatccg cgtctggtga acaaggtgca tgaaggccgt ccgcacattc aggaccgcat
    15721 caagaatggc gaatatacct acatcatcaa caccacttca ggccgtcgcg cgattgaaga
    15781 ctcccgcgtg atccgtcgca gtgcgctgca atataaagtg cattatgaca ccaccctgaa
    15841 cggtggtttc gctactgcga tgg
//
LOCUS       DAJTVJ010000096        15764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000096 DAJTVJ010000000
VERSION     DAJTVJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.096"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..3122
                     /locus_tag="OO448_003002"
     CDS             <1..3122
                     /locus_tag="OO448_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001745227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6338155.1"
                     /translation="RVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKR
                     VVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYG
                     YNNQLQLTSVTYPDGLRSSRKYDRQGRLAEETSRNGNITRWFYDSSRSGLPCAVEDGT
                     GVRRRITRNRYGQLQAFTDCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVS
                     QRDAQGRETRYEYSAAGDLTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAG
                     RITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHY
                     DASDRITRRTVNGEPAEQWQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTV
                     ETPETGEMLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEY
                     TRDRLHRETARSFGGEAYELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGP
                     QESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRI
                     AEDAHYVYRYDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQ
                     VESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQTGTTRIQ
                     TVYQPGSFTPLLRIETENGEQAKARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRA
                     GAVSAESEAWLAQCGLTAEQMAAQLEAEYIPERKLHLYHCDHRGLPLALISPEGETAW
                     QGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIG
                     LRGEWNLYKYPLNPVRFIDSLGLKFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSE
                     ETINIEYIEGTNVRFNSNNMTIYWNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCL
                     LDKENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQ
                     TMKITGTPE"
     gene            3134..3583
                     /locus_tag="OO448_003003"
     CDS             3134..3583
                     /locus_tag="OO448_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338156.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
     gene            3845..4981
                     /locus_tag="OO448_003004"
                     /pseudo
     CDS             3845..4981
                     /locus_tag="OO448_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            5151..5429
                     /locus_tag="OO448_003005"
     CDS             5151..5429
                     /locus_tag="OO448_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6338157.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            5429..5806
                     /locus_tag="OO448_003006"
     CDS             5429..5806
                     /locus_tag="OO448_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338158.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            6275..7411
                     /locus_tag="OO448_003007"
     CDS             6275..7411
                     /locus_tag="OO448_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6338159.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(7453..8223)
                     /gene="yafV"
                     /locus_tag="OO448_003008"
     CDS             complement(7453..8223)
                     /gene="yafV"
                     /locus_tag="OO448_003008"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6338160.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            8404..8850
                     /gene="ivy"
                     /locus_tag="OO448_003009"
     CDS             8404..8850
                     /gene="ivy"
                     /locus_tag="OO448_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6338161.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(8893..11337)
                     /gene="fadE"
                     /locus_tag="OO448_003010"
     CDS             complement(8893..11337)
                     /gene="fadE"
                     /locus_tag="OO448_003010"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6338162.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            11577..12155
                     /gene="lpcA"
                     /locus_tag="OO448_003011"
     CDS             11577..12155
                     /gene="lpcA"
                     /locus_tag="OO448_003011"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6338163.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            12361..13128
                     /locus_tag="OO448_003012"
     CDS             12361..13128
                     /locus_tag="OO448_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6338164.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13099..13839)
                     /locus_tag="OO448_003013"
     CDS             complement(13099..13839)
                     /locus_tag="OO448_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6338165.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(13995..14273)
                     /gene="yafQ"
                     /locus_tag="OO448_003014"
     CDS             complement(13995..14273)
                     /gene="yafQ"
                     /locus_tag="OO448_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCR6338166.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(14276..14536)
                     /gene="dinJ"
                     /locus_tag="OO448_003015"
     CDS             complement(14276..14536)
                     /gene="dinJ"
                     /locus_tag="OO448_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCR6338167.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            14722..15495
                     /locus_tag="OO448_003016"
     CDS             14722..15495
                     /locus_tag="OO448_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6338168.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         4233 a         3637 c         4148 g         3746 t
ORIGIN      
        1 gtcgggtgac ggagcaggtc aacccggagg ggctggacta ccgctttgag tacggagaga
       61 gccgggtaat catcacggac agcctgaacc ggcgggaggt gctgtacacg gaaggcgagg
      121 gtggcctgaa gcgggtggtg aagaaggagc atgccgacgg gagcatcacc cgcagcgagt
      181 atgatgaggc ggggaggctg aaggcgcaga cggatgcggc ggggcggcgg acggagtacc
      241 ggctgcatat ggcgtctgga aagctgactt cagtggtact gccggacggc aggacggtac
      301 ggtatggtta caacaaccag ttgcaactga cgtcagtgac atatccggat ggtttacgca
      361 gcagccggaa atatgaccgt cagggcaggc tggcggaaga gacctcgcgc aacggtaaca
      421 ttacgcgctg gttttatgat tcttcccgca gtggcctgcc ctgtgcggtg gaagacggaa
      481 ccggggtgag gcggagaata acgcgaaacc ggtacggtca gcttcaggcg tttacggact
      541 gctcggggta cacgacgcgg tatgagtatg accggtacgg tcaacaaatc gccgttcacc
      601 gggaagaagg catcagcact tacagcagtt ataacccgcg cggtcaactg gtcagtcaga
      661 gggatgcgca ggggcgtgaa acccgttatg agtacagcgc cgcaggagac ctcaccgcta
      721 tcgttgcccc ggacggcagc cgcagtgaga tacagtatga tgcgtgggga aaggccgtca
      781 gcaccacgca gggcggtctg acgcgaagca tggggtatga cgccgccggg cgcatcactg
      841 tgctgaccaa cgagaacggc agccagtcca cgttccggta tgacccggtc gacaggctga
      901 cggagcagcg cggctttgac ggccggacgc aacgttacca gtatgacctg accggaaaac
      961 tcacgcagag tgaagatgag gggcttatca ccctctggca ttacgatgcg tcggaccgta
     1021 tcacccggcg cacggtgaac ggtgaaccgg cggagcagtg gcagtatgac gaccacggct
     1081 ggctgacaga aatcagccac ctgagtgaag gccaccgtgt ggcggtccac tacggctatg
     1141 acgataaagg ccgtctgacg ggcgaacggc agacggtgga gaccccggag acgggggaga
     1201 tgctgtggga gcatgagacg gggcacgcgt acagcgaaca ggggctggcg acccgtcagg
     1261 agccggacgg cctgccgccg gtggagtggc tgacgtatgg cagcggttat cttgcgggga
     1321 tgaagctggg cggaacaccg ctggtcgagt acacgcggga ccggctgcac cgtgagacgg
     1381 cccgcagctt cggcggggag gcatatgaac ttgccaccgc ctggaatacc agcggccagc
     1441 tccggagcag gcacctgaac cttccgcagc ttgaccgtga ctacgactgg aacgacaacg
     1501 gacagctaat ccgcatcagt ggcccgcagg agagccggga gtaccgttac agcgacacgg
     1561 ggaggctgac gggcgtccac accacggcag cgaacctgga tatcgatatc ccgtatgcaa
     1621 cagacccggc aggaaatcgg ctgccggacc cggaactgca cccggacagc accctcacgg
     1681 cgtggccgga taaccgcatc gcggaagatg cgcactatgt ctatcgctac gatgaatacg
     1741 gcaggctggc ggagaagacg gaccgtatcc cggaaggggt tatccggatg cacgacgagc
     1801 gcacccacca ctatcactac gacagccagc accgcctggt gttctacacg cggatacagc
     1861 acggcgaacc acaggtggag agccggtacc tctatgaccc gctgggccgc cggacgggaa
     1921 aacgggtatg gcggcgggag cgtgacctga cggggtggat gtcgctgtcg cgtaaaccgg
     1981 aggtgacatg gtacgggtgg gacggtgaca ggctgacgac gatacagacc ggcaccacgc
     2041 gtatccagac ggtataccag ccggggagct tcacgccgct cctgagaatc gaaacagaga
     2101 atggcgaaca ggcgaaggcg cggcaccgga gcctggcgga ggtgttgcag gaggacacgg
     2161 gtgtgacgct accggcggag ctgtcggtga tgctggggag gctggagcgg gaactgcgcg
     2221 cgggagcggt gagtgcggag agtgaggcat ggcttgcgca gtgcgggctg acggcggagc
     2281 agatggccgc gcagctggag gcggaataca tcccggagag gaaacttcat ctttaccact
     2341 gcgaccaccg gggactgccg ctggcgctca tcagcccgga aggggaaacg gcgtggcagg
     2401 gggagtatga cgaatgggga aacctgctgg gcgaaaccag cgcgcagcac ctccagcagt
     2461 cactccgtct gccggggcag cagtatgatg aggagtcggg gctgtactac aaccgcaacc
     2521 ggtactatga cccgttgcag ggaaggtata tcacccagga tccgataggg ctgagaggtg
     2581 aatggaatct gtataaatat ccattaaacc ctgttcggtt tatagattct ttgggtttaa
     2641 aattccatgt taacggtgat ccaagtgatt ttaatcaggc tgttgagtat ttaaaacaag
     2701 attcacaaat gaaagaaact atagattttt tatcaagctc tgaagaaact ataaatatag
     2761 agtatattga aggaaccaat gttagattta attcgaataa tatgacgata tattggaata
     2821 gtagagcatc tttattttgt tcaactgaat taaactcgaa atcacaatcg cctgcattag
     2881 gtttgggaca tgagtttact catgcgcagt attgccttct tgataaggaa aatttcatgg
     2941 ctcttttaag ccgaacagat aagaaatatg agaataaaga agaggcaagg gttattacaa
     3001 taattgaatc gcgtgcggcg aaaactttag gtgagtgtac cagaggagct catagtggac
     3061 tgccttttta tcgggtggat ggaccgttgc agacgatgaa aataacgggt acgccagaat
     3121 agtgaggttt tttatgatga aatatttgat ggttttattg tctctatttt ctggttcggt
     3181 attggggatg gggcgagtga atgaattgtg tggaatagat agcgtgaaaa cgatagagat
     3241 aattaatctt ccatcgtatg ttaccacgtt agttccgctt agcaaggaag gtcttaatga
     3301 aatatacaga tataaagtgg tggtgaatga aattagagat ctttatgctg gtaagataat
     3361 tgatttattg caaatgaagt atttcagaaa agaaaaatat aacaatataa gatggggggg
     3421 tagtatcatt tcgaaaggta ataataaatg tgaaatttat tttgatgctt ttggtgaatg
     3481 tggaagtgtg aatggcatta acgtgtgttt tgaaaaaaat gaaatgatag gttggattaa
     3541 aaaagaaatc cctttgctgt cgcaaaaaat aggtgggctt taaatggtag ggcaatattc
     3601 aaagacagta taggtaacta tttgtatggc catgtaaggt aaaagaaaag gaataaacca
     3661 tgagcggaaa accggcggcg cggcagggcg acatgacccg cagggaaaga tcatcagaag
     3721 ttcacttttt gtactaaata attcgcattt tatgtttaaa aattgagata ttccttatta
     3781 cctaaagctg ttttttattg cttatacatg atcaaatact tcttacataa ttaaggagaa
     3841 caaaatggaa cttaaaaaat tgatggaaca tatttctatt atccccgatt acagacaagc
     3901 ctggaaagtg gaacataaat tatcggatat tctactgttg actatttgtg ccgttatttc
     3961 tggtgcagag ggttgggaag atatagagga ttttggggaa acacatctcg attttttgaa
     4021 gcaatatggt gattttgaaa atggtattcc tgttcacgat accattgcca gagttgtatc
     4081 ctgtatcagt cctgcaaaat ttcacgagtg ctttattaac tgaatgcgtg actgccattc
     4141 ttcagatgat aaagacgtca ttgcaattga tggaaaaacg ctccggcact cttatgacaa
     4201 gagtcgccgc aggggagcga ttcatgtcat tagtgcgttc tcaacaatgc acagtctggt
     4261 catcggacag atcaagacgg atgagaaatc taatgagatt acagctatcc cagaacttct
     4321 taacatgcta gatattaaag gaaaaatcat cacaactgat gcgatgggtt gccagaaaga
     4381 tattgcagag aagatacaaa aacagggagg tgattattta ttcgcggtaa aaggaaacca
     4441 ggggcggcta aataaagcct ttgaggaaaa atttccgctg aaagaattaa ataatccaga
     4501 gcatgacagt tacgcaatga gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat
     4561 tgtttgcgat gtccctgatg aacttattga tttcacgttt gaatggaaag gactgaagaa
     4621 attatgcgtg gcagtctcct ttcggtcaat aatagcagaa caaaagaaag agccagaaat
     4681 gacggtcaga tattatatca gttctgctga tttaaccgca gaaaagttcg ccacagcaat
     4741 ccgaaaccac tggcacgtgg agaataagct gcactggcgt ctggacgtgg taatgaatga
     4801 agacgactgc aaaataagaa gaggaaacgc cgcagaatta ttttcaggga tacggcacat
     4861 cgctattaat attttaacga atgataaggt attcaaggca gggttaagac gtaagatgcg
     4921 aaaagcagcc atggatagaa actatctcgc gtcagtcctt gcggggagcg ggctttcgta
     4981 atctttccct agggagtcag ccgaatgttg ggaaggattt gacggatgaa gagaagaact
     5041 gcttggccgg cggcagtacg ggaagcccgg gaggatgggg accagaagat gagaatagcg
     5101 caagggatcg tatgcagcat gaacggtata aagatgaatt gcgacagaaa atgggaaaac
     5161 ccgacgttac agattcaaat ttgaaaaata ttatagacga tctttatcgg ccaaatgcaa
     5221 aagtaggaag tggaagcaca gcagatgctg ttcgttatga attggccaca ggtgaaaaag
     5281 ttggtggtag aggacatgta ttgaaagcac aggattatac aagggcttta caggattggt
     5341 tggataagaa tccaacagca tctccagggg atcgagcagc agcagaaaac gttcttcagg
     5401 atttacagaa tgctttggga ggcaagtaat gaaccaatat attgctagtt atatagaaaa
     5461 aatggcatca ttttccactg ttttgtctga gacagtctca ttagctaatg aatactggca
     5521 tccagaaacg ccaccggtgg ttatgttgtt ctctcaggtt ggaaaaacga ttgtaaaaaa
     5581 tttttatgaa atgggggata ttgaaagaga aaaaatattt aaacatgtcg aagatggtat
     5641 ggtctcgctt gatgatgaat tggccacagt tgtagctaca gggcttatag aggcaatagt
     5701 cactgcgacc gatagcaatt ctttactttg gcaagattta agtaaatttc tgggtgacaa
     5761 atcaaagtct catgcattag tgtggaaaaa ttttggtcag gagtgatatt attcaagaag
     5821 tattaagctc gctattgaga tcttaatgct tcttgagatt aaattaaagt taggacgaag
     5881 taattgcagg tggaaataaa tccatagtga cgtgtggcaa tggctattag atgttatggt
     5941 gtgatgggct ttggtgattt aagagctaag ttagattgcc ttatgaaaaa gtatggttat
     6001 atatttaatg atgtgtatca tatatttaaa taaaaaacgg ttctctcttg tcaagatagt
     6061 aataggatag actattgtgt tgataaatat aattaagagt ctactttgtt gattttagaa
     6121 gactttggag cagggaaaga tcatcggaag tacacttttt gtactaaata attcgcgcta
     6181 aatgttcaat aattgagata ttccttatta cctaaaacta tttttcatag cttatatatg
     6241 atcaaatact ccttgcataa ataaggagaa caaaatggaa cttaaaaaac tgatggaaca
     6301 tatttctatt atccccgatt acagacaagc ctggaaagta gaacataaat tgtcagatat
     6361 tctactgttg actatttgtg ccgtcatttc tggtgcagag ggttgggaag atatagagga
     6421 ttttggtgaa acacatctcg attttttgaa gcaatatggt gattttgaaa atggtattcc
     6481 tgttcacgat actattgcca gagttgtatc ctgtatcagt cctgcaaaat ttcacgagtg
     6541 ctttattaac tggatgcgtg actgccattc ttcagatgat aaagacgtca ttgcaattga
     6601 tggaaaaacg ctccggcact cttatgacaa gagtcgccgc aagggagcga ttcatgtcat
     6661 tagtgcgttc tcaacaatgc acagtctggt tctcgggcag attaagacgg atgagaaatc
     6721 caatgagatt acagccattc ctgaacttct taacatgctg gatattaaag gaaaaatcat
     6781 cacaactgat gcgatgggat gccagaaaga tattgcagag aagatacaaa aacagggcgg
     6841 tgattattta ttcgcggtaa aaggaaacca ggggcggctt aataaagcct ttgaggaaaa
     6901 atttccgctg aaagaattaa ataatccaga gcatgacagt tacgcaatca gtgaaaagag
     6961 tcacggcaga gaagaaatcc gtcttcatat tgtttgcgat gtccctgatg aacttattga
     7021 tttcacgttt gaatggaaag ggctgaagaa attatgcgtg gcagtctcct ttcggtccat
     7081 aatagcagaa caaaagaaag agccagaaat gacggtcaga tattatatca gttctgctga
     7141 tttaaccgca gagaagttcg ccacagcaat ccgaaaccac tggcacgtgg agaataagct
     7201 gcactggcgt ctggacgtgg taatgaatga agacgactgc aaaataagaa gaggaaatgc
     7261 agcagaatta ttttcaggga tacggcacat cgctattaat attttgacga atgataaggt
     7321 attcaaggca gggttaagac gtaagatgcg aaaagcagct atggacagaa actatctggc
     7381 gtcagtcctt gcggagagcg ggctttcgta gtcttacccc gactctcccc cagccttaaa
     7441 cacaaccccc actcaccgca acctaaactc atccgcatcc tgccatgccg gaaacttttc
     7501 tctatattcc cgcaatgcca tcatcgacag ctccgcatca atgcgcgttg cctgatgcgc
     7561 gtcggcagta gcgataatct cgccttgtgg attaatcacc cggctgtcac cgcgataatg
     7621 gcagccgttg ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat
     7681 cgcgcgggcc gtcagcaatg cctgccagtg cagagagcgc ggagcaggcc agttggcaac
     7741 gtagatggcg aggtcataat cgttgagatt gcgcgaccac accggaaaac gtaagtcgta
     7801 gcacaccaac ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc
     7861 tttataatgt agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac
     7921 cgtgccgccc ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc
     7981 aacactgcct gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agtccactac
     8041 gtcatcttgt gctagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctt
     8101 cggtagaacg atcacatcgc gcccggtaat accttccagt tgacgatcaa aatgacgcag
     8161 gttggcaggg ccatccatcc acaccagtgg ttgctgcaaa agcgtaatct tcaaaccagg
     8221 cacggtgtac aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt
     8281 gccttagcgc agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat
     8341 tatcgtgtta tcgccaggct ttagaaggtt aataacatgg gcaggataag ctcgggagga
     8401 atgatgttta agacaataac gacagtcgcc gctctggtca tcgccaccag tgcaatggcg
     8461 caggatgatt taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcatttaat
     8521 cagatggtac aagggcataa gctgcctgcc tgggtgatga aaggcggtac ttatactccc
     8581 gcacaaaccg taacgttggg agatgatacg tatcaggtga tgagcgcgtg caaaccgcat
     8641 gactgtggct cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg
     8701 ctgttctcga ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac
     8761 gatgcgcttt cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg cagcctggaa
     8821 aaccatccgg atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc
     8881 tccgttattc aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt
     8941 cgtcgccagc tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc
     9001 agctttcacc agaatagcgg cttcatcttt atcaatcagc cccttcgcca gcgcgttgtg
     9061 cgccagttca tccagacggg taaacggcag gtttttaccc agctctttac agatccgctg
     9121 atgaattggg tcggcggcaa tcacatccac cagcgcctct tccagcaagc caaccggatt
     9181 atgctcgctc ggcgtcaggt actgaccgcg accaatgcgg gaacgggtgg cgttcggcac
     9241 ttgtaaaatc ttcgccactt tatgatccag cttgtcagaa ggtgccagat aatgacgtcc
     9301 ggtcgggaag atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag
     9361 taaatcatcc atcgcctgtt cagcctgata cagcgcatct tgtacgcccc agtgcaccag
     9421 cggcaggtcg gcttcattac ggccttcgtc gtcataacgc ttcagcacgg cagaggcgag
     9481 gtagagctgg cttaaaatat cccccagacg ggccgagatg cgctcgcgac gtttcaggct
     9541 gccgtccagc actgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt
     9601 caggtgctga tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg
     9661 cgtcaggccc agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa
     9721 caacagttta tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac
     9781 gtacggatgg caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt
     9841 agccccttca acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct
     9901 ttgcccgagc ataatgcctt tgccaccagt gatatccatc gcatcaataa tcgactgctg
     9961 cccgcggtgg gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag
    10021 cataatgccg taggtaatca gcgatgccgc agcatccatc acgtaggcat taccggcaat
    10081 acgcgccagc ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg
    10141 aatgtgagca tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga
    10201 aggcagggtg atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc
    10261 ggccattttc ggcccaccga tgatgtaatc gatcggtacg aagacatctt taccgcgcgt
    10321 cggtccgttc tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg
    10381 cgtggtggtc gggatcagcg cacaggtaat acctaaatct tctgcgccac ccagcagctt
    10441 ttctgggtcg gagagtttaa acgccagccc aagcacagtc gcaatcggtg ccagcgtaat
    10501 atagcgtttg ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat
    10561 gcagacaatc ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggcttgtcag
    10621 tgcaaagcaa gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc
    10681 gtcagtgccg taatgttgca gcagctcgcc cgggcctaat gagttcggca cgccgacggt
    10741 aatcgccagg atcccgctca cgccggagag tttttgcagc acgcgagact gggcataagc
    10801 tgagaactcc agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt
    10861 aaggtacgcc cacaactccg gcggcagatc cgccagctca tgagtaatct ggaaatcatt
    10921 cgccatccgg caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cggcggtcag
    10981 gcgcggctgc ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc
    11041 ctcccaccag gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat
    11101 caccttacgg aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa
    11161 gttaaatggc acgaggataa tggccagagg caccagtacc cacgctgacc acagaccagc
    11221 aacgccgagg gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg
    11281 gtgatagaac aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac
    11341 gaaaagcccc ttacttgtag gaggtctgac cacttgtgat gatatggttg tagtggatgt
    11401 aaaaacattt agcaatgtgt ttacaatata attacaacaa agctcacatt gttgctgttt
    11461 ttatccgcac ttcaggtcaa aaagtcctgg tcatagcacc tgcccgtact tctcgctttt
    11521 ggcggtatcc ggtacactgc attttgtcta ttacatttat gctgaaggat atcctcatgt
    11581 accaggatct tattcgtaac gaactgaacg aagcggcgga aacgctggct aactttttaa
    11641 aagatgacgc caatattcac gccattcagc gcgcggcggt cctgttagca gacagcttta
    11701 aagccggtgg caaagtgctt tcctgcggca acggcggttc ccattgcgac gctatgcact
    11761 ttgccgaaga gttgaccggt cgctaccgtg aaaaccgtcc gggctacccg gcgattgcta
    11821 tttctgacgt tagtcatatt tcctgcgtcg gtaatgattt cggtttcaat gatattttct
    11881 cccgctacgt tgaagcggta ggtcgcgaag gcgatgtact gctggggatc tccacctccg
    11941 gtaactctgc aaacgtgatc aaagcgatcg cagcggcgcg tgagaagggg atgaaagtga
    12001 tcaccctgac cggtaaagac ggcggtaaaa tggctggcac ggcggatatc gaaattcgcg
    12061 ttccgcactt tggttatgcc gaccgcattc aggagattca cattaaagtg atccatatcc
    12121 tgatccagtt aattgaaaaa gagatggtta agtaagtctg gcgtaggccg gataaggcgt
    12181 ttacgccgca tccggcactt gtgcgctgat gcctgatgcg acgctgatgc gtcttatcat
    12241 gcctacaaat ctgtacgcga accgtaggcc gaataagacg tttacgccgc atccggcctg
    12301 aaaattctta aatcaatctt cgccgggggc catgcgctcc cgctgttgtg gaggttaccc
    12361 atgtgcgaat tgctcgggat gagcgccaac gtccctaccg atatctgctt tagtttcacc
    12421 ggtctggtgc agcgtggtgg tggaaccggg ccacataaag atggctgggg cattaccttt
    12481 tacgaaggta aaggctgtcg cacatttaaa gatccacaac ccagctttaa ttcccccatc
    12541 gccaaacttg tccaggacta cccgataaaa tcctgttcgg tggtggctca tattcgccag
    12601 gccaatcggg gcgaggtggc gctggaaaat actcacccgt ttacccgtga gttatggggg
    12661 cgtaactgga cttatgccca taacggacaa ctgacgggct acaaatcact ggaaaccggc
    12721 aacttccgcc cggtcggtga aaccgatagc gaaaaagcct tctgctggct cctgcataaa
    12781 ttaacgcagc gttacccgcg cacaccgggc aacatggcgg cagtgtttaa atatatcgcc
    12841 tcactggcgg atgaactgcg gcagaagggc gttttcaaca tgctgctttc tgacgggcgc
    12901 tatgtgatgg cgtattgctc gactaattta cactggatca cccgccgcgc gccgtttggc
    12961 gtggcaacgc tgctggatca ggatgtggaa atcgacttca gctcgcagac cacaccgaat
    13021 gatgtggtca cggtgattgc aacacagccg ctgacgggca atgaaacctg gcaaaagatt
    13081 atgccaggcg aatggcgctt attttgcctc ggggagcgtg tagtttgatg ccagttgtgg
    13141 ctgcacaact tcgtggctta acggcttgct gaccacgtaa cgaccattga ccacagaaac
    13201 ggtcggtggc ttacgggttt gctcaaagta gtcgtaaccc ggcttcagtt gctcccagaa
    13261 gtccttaaag ttggaatatt tatggcgctt catattggcg tcggtcatgc ggaacgggta
    13321 aatactcact tgcacgctcg gctgaccaaa caccagcgca ccagtaacga actggaagat
    13381 ctcatcaata ccctgattgg tcattgcgta gcagccgatg gaaacacaat cgccgtggat
    13441 catcaggtat ttcccttcat aaccatgcgc acggtcatag gcattgggga aaccaatatt
    13501 aatcgctttg tagtaacggc tgtctggttt taactgatta cgctggacgc tataaaaccc
    13561 ttccgggctt ttgaaatcgc cctgacgctg ttttggccct aagccgccgg aatatttaca
    13621 gattttataa ctgtcgagca gctgatattg ctcgcccatt ttgacgtaga gatcgagcgt
    13681 acgttcttcc ttgaagatct ggatgtagac aggggatccc atcaactgct gcttatactc
    13741 tttgctcact ggcgtggtcg agctactgct gcccagcaaa ccggcaaacg aaacgcacgg
    13801 gatcaacaac atcgcaagaa ttaatgcgat tttacgcata ctgcttattc cttgataaaa
    13861 cggttacaca cgccaggacg gcaaaataga tcccaaatcg gaatagtctg gatttggaag
    13921 gctcacatta tcaccaaaag agttttacgc aagcctgtcg gcgcagggtt tacaaatttc
    13981 atcggcagcg tgctttaccc aaagagcgcc gcgtgagttc cagttctctc aaatcgtaaa
    14041 agtttatcgg taagtttgta aatcaggatc cagtccggtt cgacatgagc atcgcgataa
    14101 cctttccatg aaccttgcag cgggtggtct ttataaacag ctggaagcgg taaagcatta
    14161 ttgataagaa gcgtcataag atatttcaat ttattcatat ccttatgacg cttttgtgca
    14221 agttttacat cctttgaaaa ttgtcccgag tattcaatat ccctttgaat catatttaaa
    14281 ttcctaattt atcaaataaa tcatcagcgt ctttggcctt atgaacatca acgccagctt
    14341 cgctgttttt gattgattga atggttaatt gattaggctc gcgtaaatca aacggcaatg
    14401 ccttttcacg cgcgaccttt gtgagggtta tgcgaaccag gtcagagatg gtcagcccca
    14461 tcccggccag tacgtccgcc gcctgattct tcagatcttc atcgattcgg gcgcgaacaa
    14521 acgcgttagc agccattggg ggatctcctg tgctgtatat ttattcagct tgaattgtag
    14581 ctcaaatgag caacagcgac aatccatgag taatttaata aacggatgtt tttacataat
    14641 aaactgaaat atatttatta attttataat aatggtttat taagtttgtt caggtacggg
    14701 ccaggataca ctgcaacatc aatgcatgac cagccattat tgagaatgtc tttgccgtcg
    14761 attccctctt ttgtgttgtc gggattactg ttgctttgct tgccgttttc ttcatttgcc
    14821 tgcgccacca catcacatat ctctttcagc tacgccgccc gccagcggat gcaaaaccgt
    14881 gcgcgtttat taaaacagta ccaaacccat ctgaaaaaac aggccagcta cattgtggaa
    14941 ggcaatgccc aaagcagaag ggcgctgcgc cagcacaacc gggagcagat aaaacagcat
    15001 ccagaatggt ttcctgcgcc gctcaaggcg agtgacagac gctggcaggc gctggcggaa
    15061 aacaaccact ttttaagcag cgatcacctg cataacatca ccgaagtggc gattcaccgt
    15121 ctggagcagc agcttggcaa gccttacgtc tggggcggca cgcggcccga tcaaggcttc
    15181 gattgtagtg ggttagtttt ttatgcctac aacaagatcc tcgaggcgaa gctcccgcgt
    15241 acggccaatg agatgtatca ctaccgtcgg gcaaccattg tggcgaataa cgacctgcgc
    15301 cggggagatt tgctgttttt ccatatccac agccgcgaga ttgccgacca tatgggggtg
    15361 tatctgggcg acggtcagtt tattgagtca ccgcgcaccg gggaaaccat tcgggtaagt
    15421 cggttagctg aacctttctg gcaggatcat tttttgggcg cgcggcggat attgacggaa
    15481 gagacgattt tgtaggacgg ataaggcgtt tacgccgcat ccggcggtgg tgcgcagatg
    15541 cctgatgcga cgctgacgcg tcttatcatg cctacaaatc tgtgcgtttt tgcgagccgc
    15601 tttcctgtta ttccatcacc cagggaaaga tcatcagaag tacacttttt gtactaaata
    15661 attcgcattt tatgtttaaa atttgagata ttcctcatta cttgaaactg ttttttaatg
    15721 cttatgcatg atcaaatact ccttacataa ttaaggagaa caaa
//
LOCUS       DAJTVJ010000097        15694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000097 DAJTVJ010000000
VERSION     DAJTVJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.097"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1453
                     /locus_tag="OO448_003017"
                     /pseudo
     CDS             1..1453
                     /locus_tag="OO448_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="OO448_003018"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="OO448_003018"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6338169.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="OO448_003019"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="OO448_003019"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6338170.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="OO448_003020"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="OO448_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6338171.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="OO448_003021"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="OO448_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6338172.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="OO448_003022"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="OO448_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6338173.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="OO448_003023"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="OO448_003023"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6338174.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="OO448_003024"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="OO448_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6338175.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="OO448_003025"
     CDS             complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="OO448_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6338176.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8572..9174
                     /locus_tag="OO448_003026"
     CDS             8572..9174
                     /locus_tag="OO448_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6338177.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="OO448_003027"
     CDS             complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="OO448_003027"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6338178.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9732..10472)
                     /locus_tag="OO448_003028"
     CDS             complement(9732..10472)
                     /locus_tag="OO448_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6338179.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="OO448_003029"
     CDS             complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="OO448_003029"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6338180.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="OO448_003030"
     CDS             complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="OO448_003030"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6338181.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13153..13719
                     /locus_tag="OO448_003031"
     CDS             13153..13719
                     /locus_tag="OO448_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6338182.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14074..14322
                     /locus_tag="OO448_003032"
     CDS             14074..14322
                     /locus_tag="OO448_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6338183.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14923..>15694
                     /locus_tag="OO448_003033"
     CDS             14923..>15694
                     /locus_tag="OO448_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6338184.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH"
BASE COUNT         3985 a         3974 c         3603 g         4132 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtatag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata
     8521 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
     8581 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta
     8641 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
     8701 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
     8761 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
     8821 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
     8881 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
     8941 gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg
     9001 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
     9061 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg
     9121 attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac
     9181 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga
     9241 cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg
     9301 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
     9361 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
     9421 ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt
     9481 tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg
     9541 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     9601 atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca
     9661 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     9721 agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact
     9781 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     9841 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
     9901 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg
     9961 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
    10021 ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag
    10081 gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca
    10141 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    10201 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    10261 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    10321 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    10381 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    10441 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    10501 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    10561 cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg
    10621 gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    10681 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    10741 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
    10801 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    10861 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    10921 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    10981 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    11041 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    11101 aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    11161 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    11221 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    11281 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    11341 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    11401 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    11461 ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    11521 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    11581 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
    11641 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    11701 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    11761 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    11821 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    11881 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    11941 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    12001 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    12061 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    12121 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    12181 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    12241 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    12301 acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    12361 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    12421 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    12481 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    12541 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    12601 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    12661 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    12721 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    12781 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    12841 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    12901 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    12961 tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc
    13021 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    13081 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    13141 tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta
    13201 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
    13261 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt
    13321 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct
    13381 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
    13441 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
    13501 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
    13561 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
    13621 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
    13681 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
    13741 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
    13801 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
    13861 ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg
    13921 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
    13981 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
    14041 tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa
    14101 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
    14161 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
    14221 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
    14281 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
    14341 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
    14401 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
    14461 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
    14521 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
    14581 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
    14641 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct
    14701 cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag
    14761 aagttcttca atatataaca tatggagtta atatgcttac acaaactata tattcgcctc
    14821 ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa
    14881 gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac
    14941 aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat
    15001 gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga
    15061 gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg
    15121 tctgaattag gcctgacaac attacctgaa atccccccgg aaattaaatc aattaatata
    15181 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc
    15241 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
    15301 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
    15361 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
    15421 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
    15481 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
    15541 atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt
    15601 gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca
    15661 atagatatta gtgataaccc attgtcatcc catg
//
LOCUS       DAJTVJ010000098        15584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000098 DAJTVJ010000000
VERSION     DAJTVJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.098"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2067
                     /locus_tag="OO448_003034"
     CDS             <1..2067
                     /locus_tag="OO448_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6338185.1"
                     /translation="SGYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNS
                     GLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSV
                     RFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITD
                     LQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHS
                     DFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGD
                     VFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYD
                     IADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLR
                     RISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFAS
                     NNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQA
                     GASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMT
                     PYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQP
                     LTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDES
                     RNYICQ"
     gene            2083..3117
                     /locus_tag="OO448_003035"
     CDS             2083..3117
                     /locus_tag="OO448_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCR6338186.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="OO448_003036"
     CDS             complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="OO448_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6338187.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3756..4580)
                     /gene="rcnA"
                     /locus_tag="OO448_003037"
     CDS             complement(3756..4580)
                     /gene="rcnA"
                     /locus_tag="OO448_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6338188.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4701..4973
                     /gene="rcnR"
                     /locus_tag="OO448_003038"
                     /pseudo
     CDS             4701..4973
                     /gene="rcnR"
                     /locus_tag="OO448_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5065..5161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5196..5984
                     /gene="thiM"
                     /locus_tag="OO448_003039"
     CDS             5196..5984
                     /gene="thiM"
                     /locus_tag="OO448_003039"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6338189.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5981..6781
                     /gene="thiD"
                     /locus_tag="OO448_003040"
     CDS             5981..6781
                     /gene="thiD"
                     /locus_tag="OO448_003040"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6338190.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6846..7664
                     /locus_tag="OO448_003041"
     CDS             6846..7664
                     /locus_tag="OO448_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6338191.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7716..8462
                     /locus_tag="OO448_003042"
     CDS             7716..8462
                     /locus_tag="OO448_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6338192.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8436..9401)
                     /locus_tag="OO448_003043"
     CDS             complement(8436..9401)
                     /locus_tag="OO448_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6338193.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9398..10402)
                     /locus_tag="OO448_003044"
     CDS             complement(9398..10402)
                     /locus_tag="OO448_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR6338194.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10399..11676)
                     /locus_tag="OO448_003045"
     CDS             complement(10399..11676)
                     /locus_tag="OO448_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6338195.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11933..12985
                     /gene="fbaB"
                     /locus_tag="OO448_003046"
     CDS             11933..12985
                     /gene="fbaB"
                     /locus_tag="OO448_003046"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6338196.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13304..14158
                     /gene="gatY"
                     /locus_tag="OO448_003047"
     CDS             13304..14158
                     /gene="gatY"
                     /locus_tag="OO448_003047"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6338197.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14187..15444
                     /gene="gatZ"
                     /locus_tag="OO448_003048"
                     /pseudo
     CDS             14187..15444
                     /gene="gatZ"
                     /locus_tag="OO448_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15454..>15584
                     /locus_tag="OO448_003049"
     CDS             15454..>15584
                     /locus_tag="OO448_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6338198.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4089 a         3728 c         3988 g         3779 t
ORIGIN      
        1 agtggctatg ttccaccgga aaactgggag cggggtatta atgcgtttta tacctcttat
       61 tatgtgagtc agtattacag tgactataaa gcgtcgggta atagtaagag tacatatgta
      121 cgttttaaca gcgggttaaa tttactgggg tggcaactgc attctgatgc cagtttcagt
      181 aaaacaaata acaatccagg ggtgtggaaa agcaataccc tgtatctgga acgtggattt
      241 gcccaacttc tcggcacgct tcgcgtgggt gatatgtaca catcaagcga tatttttgat
      301 tctgttcgct tcagaggtgt gcggttgttt cgtgatatgc agatgttgcc taactcgaaa
      361 caaaatttta cgccacgggt gcaggggatt gctcagagta acgcgctggt aactattgaa
      421 cagaatggtt ttgtggttta tcagaaagag gttcctcctg gcccgtttgc gattacagat
      481 ttgcagttgg ccggtggtgg agcagatctt gatgtcagcg tgaaagaggc ggacggttcg
      541 gtaaccacct atctggtgcc ttatgcagcg gtgccaaata tgctgcaacc cggcgtgtcg
      601 aaatatgatt ttgcggcggg tcgtagccat attgaagggg cgagcaaaca tagtgatttt
      661 gtccaggcgg gttatcagta tggttttaat aacttattga cgttgtatgg tggctcgatg
      721 gttgcgaata attattacgc gttcactttg gggactggct ggaatacacg cattggtgcc
      781 atttccgtcg atgccacgaa gtcgcatagt aaacaagaca acggcgatgt gtttgacggg
      841 caaagttatc aaattgccta caacaaattt gtgagccaaa cgtcgacgcg ttttggtctg
      901 gcggcctggc gttattcgtc gcgtgattac cggacattta acgatcacgt ttgggcaaac
      961 aataaagata attatcgccg tgatgaaaac gatgtctatg acattgccga ttattaccag
     1021 aatgattttg gtcgcaaaaa tagcttttcc gccaatatga gccagtcatt gccagaaggt
     1081 tgggggtctg tgtcattaag tacgttatgg cgagattact gggggcgtag cggcagtagt
     1141 aaggattatc agttgagtta ttccaacaac ctgcgacgga taagctatac cctcgcggca
     1201 agccaggctt atgacgagaa tcatcatgaa gagaaacgtt ttaatatttt tatatcgatt
     1261 ccctttgatt ggggtgatga cgtttcgacg cctcgtcggc aaatatatat gtctaactca
     1321 acgacgtttg atgatcaggg gtttgcctca aataatacgg gattatcagg aacagtaggg
     1381 agtcgggatc agttcaatta tggtgtcaac ctgagtcatc aacatcaggg aaatgaaacg
     1441 acagctggag cgaatttaac ctggaacgcg ccggtggcaa cagtgaatgg cagttatagt
     1501 cagtcgagta cttatcgaca ggctggagcc agtgtttcag ggggcattgt cgcctggtcg
     1561 ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg ctgtgatgaa tgcgccagga
     1621 attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa caaaccgtaa tggagtggtg
     1681 gtatacgacg gaatgacacc ttatcgggaa aatcacctga tgctggatgt gtcgcaaagc
     1741 gatagcgaag cagaattacg tggcaaccgg aaaattgccg caccttatcg cggcgcggtt
     1801 gtactggtta attttgatac cgatcagcgc aagccctggt ttataaaagc gttaagagcg
     1861 gatgggcaac cattaacgtt tggttatgaa gtcaatgata tccacggtca taatattggc
     1921 gttgtcggcc agggaagcca gttatttatt cgcaccaatg aaataccgcc atcggttaat
     1981 gtagcaattg ataagcaaca aggactttca tgcacaatca ccttcggtaa agagattgat
     2041 gaaagtagaa attatatttg ccagtaatag tcaggtggtt ctatggaaat tcgcataatg
     2101 ctatttatat taatgatgat ggttatgcct gtgagctatg cggcatgtta tagtgagtta
     2161 tctgttcagc acaatttggt tgttcagggg gattttgcac ttactcaaac acaaatagcg
     2221 acatatgagc ataattttaa tgattcgtca tgcgtgagta caaatactat cacccctatg
     2281 agttcgtcgg atattattgt tgggctttat aacgatacca taaaattaaa tttacatttt
     2341 gaatggacca ataaaaacaa catcacgttg tcaaataatc agaccagttt caccagtggt
     2401 tattcagtta cggtgacacc tgcggccagt aatgcaaaag tgaatgtttc tgcggggggc
     2461 ggcggttcag tgatgattaa tggtgttgcg acattatcca gtgcttcatc atcgacacgc
     2521 gggagtgccg cagtacaatt tctactgtgt ttattaggtg gcaagtcatg ggatgcatgt
     2581 gtaaatagct acagaaatgc attggcacaa aatgcaggtg tctattcctt taatctgaca
     2641 ttgtcataca acccgataac cacaacctgc aaaccggacg atttattaat tactttagac
     2701 agtattcccg tttcacaatt accagccaca ggtaacaaag caacaataaa tagtaaacaa
     2761 ggggatatta ttctgcgttg taaaaattta ttaggtcaac aaaatcaaac atcacggaaa
     2821 atgcaggtgt atttatcaag ttctgatttg ttagccggca gtaacacgat cctgaaaggt
     2881 gcagaaaata atggagtcgg atttatgctt gaaagcaatg gttctccagt cacattttta
     2941 aatatcactg ataacagcaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa
     3001 cttacagata caacggtttc aattccgata actgccagtt actacgtcta cgatacaaac
     3061 aaagttaaat ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa
     3121 caagcaagac gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc
     3181 atgatgaggg ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat
     3241 tttaccgtcg gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc
     3301 ggcatcaggc gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg
     3361 gtacatctct gggacggtat cacctggttt gaaatgctta aagtcagcaa tgaaactact
     3421 taactcatac ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca
     3481 ggcggcactg gtaaccagca aaagcacacc cagcaacatc ttatttttaa tggtcataaa
     3541 ctctccactt tggggatctg taatatcagt cgcatatctt tcctgccata caatataact
     3601 gacaagcgat attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc
     3661 cctaaaagca tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct
     3721 gggctgaaga taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca
     3781 tgtacgccca tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt
     3841 ttagcgagag tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg
     3901 ccaacgccta cggtgacaag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt
     3961 gcgcccagtg tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg
     4021 caggggataa ggccaccggt taagccaaat agcaaaattt gccagttggt gacctctcta
     4081 ccatcaaagc gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg
     4141 ccatgttcgt gatggtgatg atgtccatgg tcgtggtgat cttcgtgatc gtgatgatga
     4201 tgctcataat catactcgtg catattctcc agccagttgc gttcgccgcg ccaggtacgc
     4261 cagaacatcc agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt
     4321 tctgctgatt gagcagtaaa gcgcttgctg atcaccatcc cgccaaaggc aattaaccag
     4381 accactgcgg tatgcgaaat agttgctgcc agtccgagca tcaccgcttg tttaatggtg
     4441 cctttgatgg cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca
     4501 tgaagcgcac caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga
     4561 agagttgtaa attcggtcat gataataatt cttagtatta attcggcaat ctgattctac
     4621 tccccccagt acctgatact accccccagt agattaatag tgctatgata ttttcatatt
     4681 cttgttaacc aggtgttgcc atgtctcata caatccgtga taaacagaaa ctgaaagcgc
     4741 gtgccagtaa gattcagggc taggtcgtgg cgctcaagaa aatgctcgac gagccgcatg
     4801 aatgcgctgc agttttacaa cagattactg ctatccgtgg cgcggtaaac ggtctgatgc
     4861 gggaagtgat taaaggtcat ctgacggaac acatcgttca ccagggggat gagctaaaac
     4921 gtgaagaaga tctggatgtc gttctgaagg tgctggattc atatatcaaa taatttatta
     4981 acgcgattgt aaaactgccg tttttcctcg tttacaacgc gtgcgctgga cattaccatc
     5041 ctcctctgcg atttatcatc gcaaccaaac gactcggggt gcccttctgc gtgaaggctg
     5101 agaaataccc gtatcacctg atctggataa tgccagcgta gggaagtcac ggaccaccag
     5161 gtcattgctt cttcacgtta tggcaggagc aaactatgca agtcgacctg ctgggttcag
     5221 cgcaatctgc gcacgcgtta cacctttttc accaacattc ccctcttgtg cactgcatga
     5281 ccaatgatgt ggtgcaaacc tttaccgcta ataccttgct ggcgctcggt gcatcgccag
     5341 cgatggttat cgaaaccgaa gaggccagtc agtttgcggc tatcgccagt gccttgttga
     5401 ttaacgttgg cacactgacg cagccacgcg ctcaggcgat gcgtgctgcc gttgagcaag
     5461 caaaaagctc tcaaacaccc tggacgcttg atccagtagc ggttggtgcg ctcgattatc
     5521 gccgccattt ttgtcatgaa cttttatctt ttaaaccggc agcgatacgt ggtaatgctt
     5581 cggaaatcat ggcattagct ggcattgcta atggtggacg gggagtggat accactgacg
     5641 ccgcagctaa cgcgataccc gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg
     5701 tggtcactgg cgaggtggat tatgttaccg atggccatcg cgccgttggc attcacggtg
     5761 gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg
     5821 cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc atctgcctgt cactggatga
     5881 aacaagccgg agagcgcgca gtcgccagaa gcgaggggct aggcagtttt gttccacatt
     5941 tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct
     6001 gacgattgcc ggtactgatc cgagtggtgg tgcggggatt caggccgatc ttaaaacctt
     6061 ctcggcactt ggcgcttatg gttgctcagt tattactgca ctggtggcgc aaaatacccg
     6121 tggcgtacag tcggtgtatc gcattgagcc tgattttgtc gccgcccagc tcgattcggt
     6181 gttcagcgat gtgcgaatcg ataccactaa aatcggtatg ttggcggaaa ccgatattgt
     6241 tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt
     6301 tatgctggca aaaagcggcg acccactgct ttcaccttcg gcagttgcca cgctacgcag
     6361 tcgattattg ccacaggttt cattaataac gccaaacttg cccgaagcag ccgccttgct
     6421 cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa caagggcgat cgctgttggc
     6481 gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc aaagcccgga
     6541 ctggctgttt acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa
     6601 cacccacggt actggttgta cactctctgc ggcgttggct gcactacgcc cgcgccatac
     6661 aaactgggct gacaccgtac aggaggcaaa aagctggctt tcatcggcgt tagcccaggc
     6721 cgacacgctg gaagttggtc acggtattgg tccggttcac cacttccacg cctggtggtg
     6781 agcaccgtac tatagctgct ttggttattg tcagcctttg gcactgacat tcagggatga
     6841 aaccaatgca gctaagaatt accagtcgaa aaaaactcac cgctctatta tgcgctcttg
     6901 ggctaatttc catcgttgca atttatccgc gtcagacggt gaattttttc tattcgacag
     6961 cagttcagat tacagactac atccacttct acggttatcg cccggttaaa tctttcgcta
     7021 ttcgtattcc tgccagttac accattcacg gaatagatgt ttcgcgctgg caggagcgga
     7081 tcgactggca gcgtgtggca aaaatgcgcg ataacggtat tcgcttacaa tttgccttta
     7141 ttaaggcgac ggaaggcgaa aagctggtgg acccctattt ttcgcgtaac tggcaactaa
     7201 gccgcgaaaa tggcttgctg cgcggggcat atcattattt ctccccgtcg gtagcagcac
     7261 cggttcaggt gcgattattt ctgcaaacgg tagatttctc acaaggcgat ttccctgcgg
     7321 tactggacgt agaagagcga ggaaaattat cggcaaaaga attacgcaag agggtaagtc
     7381 agtggctaaa aatggtcgaa aaaagtacgg ggaaaaagcc gattatttac tcaggagccg
     7441 ttttttatca cacgaatctg gcgggctatt tcaatgaata tccgtggtgg gtggctcact
     7501 actatcaacg tcgtccggac aatgacggca tggcctggcg cttctggcag cattccgacc
     7561 gtggacaggt agatggcatc aatggtccgg tggattttaa tgtgtttaat ggcacggtgg
     7621 aggagttgca gggattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata
     7681 tactcccacc agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga
     7741 ttgcccaact gaacgaacgt attttagcgg cggataacac gccgctttat attaagtttg
     7801 ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg
     7861 gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc
     7921 aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca
     7981 acatcttcga atactcgttg aaagaagcgc gaggtttttc tcagcaggtg gtgttgcgcg
     8041 ggaaaaagcc cgatacgcta tgggttaaca agcgtgtcgt gaaatgtccc gaagaagtcg
     8101 cgcagcagct ggcggtcgaa gcaggaagtg atgtcttttt gcttaagcgt attcgctatg
     8161 tcgatgaaga agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg
     8221 ttgatgccat cgggatttca ctttatgact atttccgcag ccagcacatt tacccacaac
     8281 gtacgcgttc ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt
     8341 tagatagcaa aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc
     8401 ctattgagta cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt
     8461 agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca cgacgccact
     8521 gcattgccca gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg
     8581 ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga
     8641 atgcagccag aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc
     8701 aacggcgcgg caaatttctc ctgccattgc tcgccaagtg ttgttatctc tgcggataaa
     8761 gcaaaacgtt cggcggcaat ctcagcctct tgacgattga gcgacactaa aggtcgacag
     8821 gccatgatcc gcgccagtaa tgcatccggg atatcgccaa tacgtgggcc aaaatcgata
     8881 aacggcgtca cgtcttgcag ctcttccagc cattccacta acaattcgcc gcagggcgag
     8941 gccagttgat aaccggaaaa atagagcagg ctgccaggcg caacggttaa tcgcgccagc
     9001 cactggcgat tccactgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc
     9061 ggctcaacca gcgccagaca ccagccgtta tcaccttcgg cgttatcgat caggctgatt
     9121 aagccctctt ttgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc
     9181 aaggcattac ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag
     9241 ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca
     9301 taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg
     9361 gttaattcag gcagcagcgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac
     9421 gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagtt
     9481 ccgcctttaa cgcaggatcg atagcgttaa cgccatgcaa cgcgccgcaa attgccgtcg
     9541 ccatagcacc gatggtgtct gtgtcgccgc caaggttagc gcacaggacg gcgcagcgat
     9601 tcgggtcggt ttgtgctagt tcaaccagcg caatggcgca cggaacggac tcaatagtgc
     9661 tggtacctgc gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat
     9721 tgcgcacaat tttcagcgca atctccagcc gcgccgccag tgaggcgctg aacgtagtga
     9781 tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaggga atcaacaatc gctgaccagc
     9841 tttctccgtc aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca
     9901 gatcggattt atgggtcggg ctggacgcca gcgctacatc atcaataaag gaatcaacat
     9961 cacgcgccgg aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggtcacgc
    10021 cgttgttttc cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg
    10081 aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg cagcgcccag tcgagaatat
    10141 tacgcccaat cagatccgga tcgatcttgc cttcacgttc cagtaacgca tccgccagac
    10201 acagcgccat cgaggtatcg tcggtgaatt cggcgcggtt aaaataacag gccgcgttat
    10261 tctcctttgg tccaggaaga aaacggtcaa tccagccaaa gtgtgcttta acgcggctgc
    10321 gtggccaaag ctcggagggc atccccatcg catcccctaa cgcctgccca taaagagcac
    10381 cgagaatacg ttctgttttc atttaacttc cccttgtgtc aacgcaatat cgcgatcatc
    10441 gaccttgata gccgtaattt cgttgtcgga ttcgcggaaa aaaatcatga acagcacggc
    10501 gataatggca atcatcaccg cgccgaaagt ccacatcccg gaccagttga aagtcagtcc
    10561 gtttaccggt tcctgataag cgaacatctt ttccatcatc acaccgccaa gacgatagcc
    10621 gagcaaactg ccgaagccct ggcagcagag cgtgatcagc ccctgcgcag cggtacgcat
    10681 atgcacgggg gcttttttat cgacatagat gtaagcggta acgtagtaaa aatcgtaact
    10741 tacgccgtga agcaaaatac cgaggaacag taacgcgtag gtgaaatatt catccgtact
    10801 accgtaaata aagaagccat agcggatcgc agcggtgacc agaccaagca ataatacctt
    10861 tttgatacca aagcgtttag tgaaaaacgg caatgccagc ataaagaaga tttcagagaa
    10921 ctggccgagc gtcatccagc cggtggcgtt tttcatgcca acttcggtca gataaccgtt
    10981 ggcaaagatg taatagaacg ccagtggcat cgcaaacagg aatgaacaga agaaaaagac
    11041 gaggaagttt ttatcgcgca gcaggatcag cgcatccagg ccgagcatga ctttaatatc
    11101 cattttgccg gtgctttttg gtggcgtgtc gggcaggaaa aacgcaaaca caccgagcag
    11161 agcagaactt ccggcggtaa tcagcagcgg gatgttagtc ggtgagatat cggcataccc
    11221 cagtatttgc ggcaagaaac cacatgccag accggaggca atccagccga tagtgcccat
    11281 cacacgaatg cgcgggaaat cacgctccac atccggcacg ttggcaaaag cgatgctgtt
    11341 agtcagcgca atggtcggca tataggttag agagtaagcc agcagtaacg gaaagaaccc
    11401 ggcaaaagtg gtctgttgcg cagcgaaata catcagcagc gcgcctgcga acatcaatac
    11461 cgccagcact ttttgcgccg agaaaaagcg gtcagtgatg gagccaacca gaattggcga
    11521 caggatcgcc gcaatggcgg tacaggcata cgaccagcca atttctccgg cactaaaacc
    11581 gcttttactt aaccacaacc acaatggcac aaaccacgcg ccccagataa accattcaac
    11641 aaacatcatg aacgacagct ttgctgttgt tttcatcttt taattccttc atgacaggta
    11701 atgtaaggta cgcgatgaca ataccactta acaatacctt ttaaatacct ttgcggtgaa
    11761 aacttgaccg cgctaacatt ttttctgatg aatcgagcca acagaaaacg ctgaaaaaac
    11821 atccaaaaga tggaaaaact cgcccgtaac gaccaggatt atgattgccc gcaaaaggcg
    11881 ggccaggaca atccatagcc gatatccaat cggaatttac gggagcatag taatgacaga
    11941 tattgcgcag ttgcttggca aagacgccga caacctttta cagcaccgtt gtatgacaat
    12001 tccttctgac cagctttatc tccccggaca tgactacgta gaccgcgtaa tgattgacaa
    12061 taatcgcccg ccagcggtgt tacgtaatat gcagacgttg tacaacaccg ggcgtctggc
    12121 tggcacagga tatctttcta ttctgccggt tgaccagggc gttgagcact ctgccggagc
    12181 ttcatttgct gctaacccgc tctactttga cccgaaaaac attgttgaac tggcgatcga
    12241 agcgggctgt aactgtgtgg cgtcaactta cggcgtgttg gcgtcggtat cgcggcgcta
    12301 tgcgcatcgc attccatttc tcgtcaaact taatcacaac gagacgctaa gttacccgaa
    12361 tacctacgat caaacgctgt atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc
    12421 ggttggtacg actatctatt ttggctcgga agagtcacgt cgccagattg aagaaatttc
    12481 tgcggccttt gaacgcgcgc acgagctggg catggtgacc gtgctgtggg cctatttgcg
    12541 aaactccgcc tttaagaaag atggcgttga ttaccatgtt tccgccgacc tgaccggtca
    12601 ggcaaaccat ctggcggcaa ccatcggtgc agatatcgtc aaacaaaaaa tggcggaaaa
    12661 taacggcggc tataaagcaa ttaattacgg ttacaccgac gatcgtgttt atagcaaatt
    12721 gaccagcgaa aacccgattg atctggtgcg ttatcagtta gctaactgct atatgggccg
    12781 ggctgggttg ataaactccg gcggtgctgc gggcggtgaa actgacctca gtgatgcagt
    12841 gcgtactgcg gttatcaaca aacgcgctgg cggaatgggg ctgattcttg gacgtaaagc
    12901 gttcaagaaa tcgatggctg acggcgtgaa actgattaac gccgtgcagg acgtttatct
    12961 cgatagcaaa attactattg cctgatacgc ctcatacgaa cagcaccacc ccgtaggacg
    13021 gataaggcgt ttacactgca tccggcaacc tatgcctgat gcgacgcgga agcgtcttat
    13081 catgcctaca tagcactgcc acgtatgtcg gaaaaggcgt ttacaccgca tccggcataa
    13141 aaacacgcgc actttgctac ggcttcccta tcgggaggcc gtttttttgc ctttcactcc
    13201 tcgaacagtt ttcatattgt cgttttgtga tcgttatctc gacatttaaa aacaaatcat
    13261 ttcattatat tttgaaatcg aaaacaagca acaggatatg aaaatgtacg tggtatcgac
    13321 aaagcagatg ctgaacaacg cacagcgcgg cggttatgcg gttccggcat tcaatattca
    13381 caatctcgaa acgatgcaag tggtggtgga aaccgctgcc aacctgcatg cgccggtcat
    13441 catcgccgga acgcctggca cattcactta tgctggtaca gaaaatctgt tggcgctggt
    13501 cagcgcgatg gcgaagcaat atcaccatcc actggcaatt catctcgacc atcacacgaa
    13561 atttgacgat atcgctcaga aggttcgttc tggcgtgcgc tcagtcatga ttgacgcctc
    13621 gcatttgcct tttgcgcaaa atatttcacg ggtcaaagag gtggtggatt tttgccatcg
    13681 ctttgatgtc agcgtcgaag cggagctggg gcaacttggc ggccaggaag atgatgtgca
    13741 agtcaatgaa gccgatgttt tttataccaa ccccgctcag gcgcgtgaat ttgccgaggc
    13801 aaccggaatt gattccctgg cggtcgccat cggcacggct catgggatgt atgccagcgc
    13861 accggcgctt gatttttctc gactggagaa cattcgccag tgggtgaact taccgctggt
    13921 gctgcatggc gcgtcagggt tatcgactaa ggatattcag caaaccatca aactggggat
    13981 atgcaaaatc aacgttgcaa cggagctgaa aaatgccttc tcgcaggcgt taaaaaatta
    14041 cctgaccgag caccctgaag cgaccgatcc ccgggattat ttgcagtcgg ctaaatccgc
    14101 aatgcgcgat gtggtgagca aagtgattgc cgattgtggc tgcgagggca gggcataacg
    14161 cacctgccat ttaacaagga aaaaacatga aaacgttaat tgcccggcat aaagctggtg
    14221 aacatatcgg catatgttca gtctgttctg cccatccgtt ggttatcgaa gcggcgctgg
    14281 catttgatcg caacagcacg cgcaaagtgc tgattgaagc aacgtcaaac caggtcaatc
    14341 aatttggcgg ttataccgga atgacaccgg cagactttcg cgaatttgtt tttacgattg
    14401 ccgataaagt cggatttgca cgcgaacgca ttattctcgg cggcgatcat ctggggccaa
    14461 actgctggca gcaagaaaat gcggatgcgg cgatggaaaa atccgtcgag ctggtaaaag
    14521 catatgttcg tgctggcttc agtaaaattc atcttgatgc gtcaatgtcc tgcgcggggg
    14581 atcccatacc gttagcacca gaaacggttg cggaacgagc tgctttgctt tgctgcggaa
    14641 agtgtggcga cagattgcca gcgtgagcaa ctgagctatg tcattggcac tgaagttccg
    14701 gttccgggcg gtgaggccag cgccattcag tcagtacaca tcacccatgt tgaagatgcc
    14761 gccaatactt tacgtacgca tcaaaaggcc tttattgccc gtgggctgac agaggcgtta
    14821 acacgcgtga ttgccatcgt ggtgcagccg ggcgtggaat ttgatcacag caatattatc
    14881 cattatcagc cgcaggaagc gcagccgctg gcgcaatgga tagaaaacac ccgaatggtt
    14941 tatgaagcac attctaccga ttaccagacc cggacggctt attgggaatt agtccgcgat
    15001 cactttgcaa tattgaaagt cggtcccgca ttaacctttg ctttacgcga ggcgatattt
    15061 gcactggcac aaattgagca ggaacttatc gcccctgaaa atcgcagcgg ttgcctggcg
    15121 gtaattgaag aagtgatgct cgacgaaccg caatactgga aaaaatatta tcgtacgggt
    15181 tttaacgatt cattactgga tattcgttac agcctgtcgg atcgtattcg ttattactgg
    15241 ccgcatagtc ggattaaaaa tagcgtcgaa acgatgatgg tgaatcttga aggcgtggac
    15301 atcccactgg gcatgattag tcagtatctt cccaaacaat ttgaacgcat tcagtccggg
    15361 gaattatcag caataccgca tcagctgatt atggataaaa tttatgatgt tttgcgcgcc
    15421 tatcgctacg gctgtgcgga ataaggacgg tatatgacta acctgtttgt tcgtagcgga
    15481 atttcttttg tcgatcgtag cgaagtttta acccatatcg gtaatgagat gctcgccaaa
    15541 ggtgtggttc atgatacctg gccacaggca ttaattgcca gaga
//
LOCUS       DAJTVJ010000099        15228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000099 DAJTVJ010000000
VERSION     DAJTVJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.099"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..751
                     /locus_tag="OO448_003050"
     CDS             <1..751
                     /locus_tag="OO448_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6338199.1"
                     /translation="ESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYE
                     LDKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQE
                     VNAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLL
                     NFDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTAS
                     LQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(792..1724)
                     /locus_tag="OO448_003051"
     CDS             complement(792..1724)
                     /locus_tag="OO448_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6338200.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2088..4874
                     /gene="polA"
                     /locus_tag="OO448_003052"
     CDS             2088..4874
                     /gene="polA"
                     /locus_tag="OO448_003052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6338201.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5256..5888)
                     /locus_tag="OO448_003053"
     CDS             complement(5256..5888)
                     /locus_tag="OO448_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6338202.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6470..6979
                     /gene="yihI"
                     /locus_tag="OO448_003054"
     CDS             6470..6979
                     /gene="yihI"
                     /locus_tag="OO448_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6338203.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7168..8541
                     /gene="hemN"
                     /locus_tag="OO448_003055"
     CDS             7168..8541
                     /gene="hemN"
                     /locus_tag="OO448_003055"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6338204.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8731..8841)
                     /locus_tag="OO448_003056"
     CDS             complement(8731..8841)
                     /locus_tag="OO448_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6338205.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8953..10362)
                     /gene="glnG"
                     /locus_tag="OO448_003057"
     CDS             complement(8953..10362)
                     /gene="glnG"
                     /locus_tag="OO448_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6338206.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10374..11423)
                     /gene="glnL"
                     /locus_tag="OO448_003058"
     CDS             complement(10374..11423)
                     /gene="glnL"
                     /locus_tag="OO448_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6338207.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11597..13006)
                     /gene="glnA"
                     /locus_tag="OO448_003059"
     CDS             complement(11597..13006)
                     /gene="glnA"
                     /locus_tag="OO448_003059"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6338208.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13379..15202
                     /gene="typA"
                     /locus_tag="OO448_003060"
     CDS             13379..15202
                     /gene="typA"
                     /locus_tag="OO448_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6338209.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3964 a         3800 c         3913 g         3551 t
ORIGIN      
        1 cgagtcgaaa tttaatcaga ttaacattat caatcacggg gaagacgttg cccaaatcga
       61 tgctttcgtt gcaaaaacca tgctggatcg cgttaaagac aaagattata tcaatgtcaa
      121 tctgacctac gaacttgata agttaacaaa agggaatcag caactcggta gtggtgagtg
      181 gtcattgatt gctgaatcta ttgatccctc agcagtgcgc caatttatca tccagtataa
      241 cattgctatg cagaagcagc ttgctgtaca ccctgagtta gcaaacgatg aagttgctct
      301 gcaagaagtg aatgctgcat tgttcaaaga gtatttaccg ttattacaaa aaagtgagcc
      361 gaccattaaa caaccggtaa gatggaagaa cgcactcggc gaactaaatg ccaatctgga
      421 tatcagtatt gccgacccag ccaaatcttc atcatccaca aacaaagata tcaaattgct
      481 caattttgat gtgaagttac cgcttaatgt cgtcacagaa accgcaaaac agcttaattt
      541 atctgaagga atggatgcgg aaaaagcgca aaagcaggct gataaacaaa tcagcgggat
      601 gatgacattg ggtcagatgt ttcagttaat cacgattgac aacaataccg cctcgctgca
      661 gctgcgttat acaccgggta aagttgtgtt taacggacag gagatgagcg aagaagaatt
      721 tatgtctcgt gccggacgtt ttgttcatta aaatactcac tcgcctctgc tcattcagag
      781 gcgagtaaaa cttacttatt ctgacgctgt gatgacatga gagacgtcag aagccggtct
      841 ttttcctgcc acaatgagtt cagccattgc tgaaaatggc gcttaaagct tttgtcattg
      901 atgtagtcgc catgtaactc atcggcaata ggctgtaaat ccacatgtac gacaatccgc
      961 gtcagtttac cgcttaacat atcgaagaac ggctgacggt tattgtccgg ataacacagc
     1021 gtaacattaa gcagtttatc gaattgttta cccagtacat ttagcgccat cgcaatgcct
     1081 gccgcctttg gtggcaacaa gttttgaaaa gtggagtggg tttgctgatg tttttcttgc
     1141 gtgaaacggg agccttcaac gaaattgaca atggtggtgg gatgcaggcg aaacttttca
     1201 caagaacgcc gagtggtttc aacatcttta ccacgtcgct ccggatgacg taataaataa
     1261 gcgcgggaat aacgcttcat aaacggcata tccagcgccc agcacgccag gccaagaaat
     1321 ggcacccagg ccagctgttg cttgaggaaa tatttattca tcggaatgtg cttacgaaac
     1381 agcacgcaca gtacgacaat atctgcccaa ctacggtggt tacaaataag cagataccag
     1441 ttcttcttac ttaacccttc cagcccgtga acttcccatt gcaggtgtgg gttcagatgc
     1501 agtaataccg ccagaccttc acaccagcaa tacatcataa aatcacaaaa acgcgaaacc
     1561 tttcgccaaa tgactggcac aggtagcaat agttttacaa tcccggcaat gatgatcggg
     1621 acggaacaaa aaatagtgac caaaatggtc aatacgatac tcagcaaaag ggttatcgca
     1681 gcgagtattc tcgtcataat gaatttattc aaaaggttag ccatagacag tgcgctaaag
     1741 aagcgcaagg cgcagatttt agcagaaaac gacccaaata acggatgatc cttaaggaga
     1801 aaaataatcc atatctatcc acattagaaa aaatcccatt atctcaatta ttagggatgg
     1861 ctttattttt aactgcatga aaaacaaaga caaacatcat gctgtaaaaa gcatgataat
     1921 aaattaaaag cgatgtaaat aatttatgca caaagttatc cacatgacga tttgcgagcg
     1981 atccagaaga tctacaaaag attttcacga aaagcggtga aaaactcatg ttttcatcct
     2041 gtctgtggca tcctttaccc ataatctgat aaacaggcac ggacattatg gttcagatcc
     2101 cccaaaatcc acttatcctt gtagatggtt catcttatct ttatcgcgca tatcacgcgt
     2161 ttcccccgct gactaacagc gcaggcgagc cgaccggtgc gatgtatggt gtcctcaaca
     2221 tgctgcgcag tctgatcatg caatataaac cgacgcatgc agcggtggtc tttgacgcca
     2281 agggaaaaac ctttcgtgat gaactgtttg aacattacaa atcacatcgc ccgccaatgc
     2341 cggacgatct gcgagcacaa atcgaacccc tgcacgcgat ggttaaagcg atgggactgc
     2401 cgctgctggc ggtttctggc gtagaagcgg acgacgttat cggtactctg gcgcgcgaag
     2461 ccgaaaaagc cgggcgtccg gtgctgatca gcactggcga taaagatatg gcgcagctgg
     2521 tgacgccaaa tattacgctt atcaacacca tgacgaatac catcctcgga ccggaagagg
     2581 tggtgaataa gtacggcgtg ccgccagaac tgatcatcga tttcctggcg ctgatgggtg
     2641 actcctccga taacattcct ggcgtaccgg gcgtcggtga aaaaaccgcg caggcattgc
     2701 tgcaaggtct tggcgggctg gatacgctgt atgccgagcc agaaaaaatt gctgggttga
     2761 gcttccgtgg cgcgaaaaca atggcagcga agctcgagca aaacaaagaa gttgcttatc
     2821 tctcatacca gctggcgacg attaaaaccg acgttgaact ggagctgacc tgtgaacaac
     2881 tggaagtgca gcaaccggca gcggaagagt tgttggggct gttcaaaaag tatgagttca
     2941 aacgctggac tgctgatgtc gaagcgggca aatggttaca ggccaaaggg gcaaaaccag
     3001 ccgcgaagcc gcaggaaacc agtgttgcag acgaagcgcc agaagtgacg gcaacggtga
     3061 tttcttatga caactacgtc accatccttg atgaagaaac actgaaagcg tggattgcga
     3121 agctggaaaa agcgccggta tttgcatttg ataccgaaac cgacagcctt gataacatct
     3181 ctgctaacct ggtcgggctt tcttttgcta tcgagccagg cgtagcggca tatattccgg
     3241 ttgctcatga ttatcttgat gcgcccgatc aaatctctcg cgagcgtgca ctcgagttgc
     3301 taaaaccgct gctggaagat gaaaaggcgc tgaaggtcgg gcaaaacctg aaatacgatc
     3361 gcggtattct ggcgaattac ggcattgagc tgcgtgggat tgcgtttgat accatgctgg
     3421 agtcctacat tctcaatagc gttgccgggc gtcacgatat ggacagcctc gcggaacgtt
     3481 ggttgaagca caaaactatc acttttgaag agattgcggg taaaggcaaa aatcaactga
     3541 cctttaacca gattgccctc gaagaggccg gacgttacgc cgccgaagat gcggatgtca
     3601 ccttgcagtt gcatctgaaa atgtggccag atctgcaaaa acacaaaggg ccgttgaacg
     3661 tcttcgagaa tatcgaaatg ccgctggtgc cggtgctttc acgcattgaa cgtaacggtg
     3721 tgaagatcga tccgaaagtg ctgcacaatc attctgaaga gctcaccctt cgtctggctg
     3781 agctggaaaa gaaagcgcat gaaattgcag gtgaggaatt taacctttct tccaccaagc
     3841 agttacaaac cattctgttt gaaaaacagg gcattaaacc gctgaagaaa acgccgggtg
     3901 gcgcgccgtc aacgtcggaa gaggtactgg aagaactggc gctggactat ccgttgccaa
     3961 aagtgattct ggagtatcgt ggtctggcga agctgaaatc gacctacacc gacaagctgc
     4021 cgctgatgat caacccgaaa accgggcgtg tgcatacctc ttatcaccag gcagtaactg
     4081 caacgggacg tttatcgtca accgatccta acctgcaaaa cattccggtg cgtaacgaag
     4141 aaggtcgtcg tatccgccag gcgtttattg cgccagagga ttatgtgatt gtctcagcgg
     4201 actactcgca gattgaactg cgcattatgg cgcatctttc gcgtgacaaa ggcttgctga
     4261 ccgcattcgc ggaaggaaaa gatatccacc gggcaacggc ggcagaagtg tttggtttgc
     4321 cactggaaac cgtcaccagc gagcaacgcc gtagcgcgaa agcgatcaac tttggtctga
     4381 tttatggcat gagtgctttc ggtctggcgc ggcaattgaa cattccacgt aaagaagcgc
     4441 agaagtacat ggacctttac ttcgaacgct accctggcgt gctggagtat atggaacgca
     4501 cccgtgctca ggcgaaagag cagggctacg ttgaaacgct ggacggacgc cgtctgtatc
     4561 tgccggatat caaatccagc aatggtgctc gtcgtgcagc ggctgaacgt gcagccatta
     4621 acgcgccaat gcagggaacc gccgccgaca ttatcaaacg ggcgatgatt gccgttgatg
     4681 cgtggttaca ggctgagcaa ccgcgtgtac gtatgatcat gcaggtacac gatgaactgg
     4741 tatttgaagt tcataaagat gatgttgatg ccgtcgcgaa gcagattcat caactgatgg
     4801 aaaactgtac ccgtctggat gtgccgttgc tggtggaagt ggggagtggc gaaaactggg
     4861 atcaggcgca ctaagattcg cctgaacatg cccttttttc gtaagtaagc aacataagct
     4921 gtcacgtttt gtgatggcta ttagaaattc ctatgcaaca actgaaaaaa aattacaaaa
     4981 agtgctttct gaactgaaca aaaaagagta aagttagtcg cgtagggtac agaggtaaga
     5041 tgttctatct ttcagacctt ttacttcacg taatcggatt tggctgaata ttttagccgc
     5101 cccagtcagt aatgactggg gcgtttttta ttgggcgaaa gaaaagatcc gtaatgcctg
     5161 atgcgctatg tttatcaggc caacggtaga attgtaatct attgaattta cgggccggat
     5221 acgccacatc cggcacaagc attaaggcaa gaaaattatt cgccgtcctg cgtttcttct
     5281 acaggctgca tctcgctaaa ccaggtatcc agtttctgcc gcagcttgtc cacgccttgt
     5341 ttcttcaacg aagaaaacgt ttcaacctgc acatcaccgt taaacgccag tacagcttca
     5401 cgcaccatat tcaattgcgc tttacgtgcg ccgcttgcca gtttgtccgc tttggtcagc
     5461 agcaccagaa cggcgatatt gctgtctacc gcccactcaa tcatctgctg atccaaatct
     5521 ttcagcggat ggcgaatatc cattagcacc accagacctt gcaggctctg acgtttttcg
     5581 aggtattcgc cgagcgcacg ctgccatttg cgcttcatct cttccgggac ttccgcataa
     5641 ccgtacccag gcaagtcaac cagacgcttg ccgtcagcca cttcaaacag gttgataagc
     5701 tgggtgcgcc ctggggtttt tgaggtacga gccaggcttt tctggttagt cagcgtgttc
     5761 agcgcgctgg atttacctgc gttggaacgg cctgcaaaag ccacttcaat tccggtatcg
     5821 gaaggtaggt ggcgaatatc aggcgcactc atcacaaaat gcgtctgttg ataattcaaa
     5881 ttagtcaaag cggtcgtctc cgtcagtcaa agctttgtgg cgattatacc tgaaccataa
     5941 taaaaggcgg atttttcggc gtgagcgttg taagtaaaag ccatacgctt tgtgagacat
     6001 tgccgatagt ctttatgcga aatagcagag aaaattctgc gatgcatgtc aaataagcta
     6061 tataaatcag tgaattgctt ttatataaaa ctctgaaaat gtgcaaatac tgatggcggt
     6121 tgattgtttg tttaaagcaa aggcgtaaag tagcacccat agagcgagga cgctaacagg
     6181 aacaatgact caggatgagg gtcaggagcg ccaggaggcg aagacagagg attgtcagga
     6241 agacaaacgt ccggagacgt aattaaacgg aaatggaatc aacacggatt gttccggcta
     6301 aaggaaaaac agggtgtgtt ggcggcctgc aaggattgta agacccgtta agggttatga
     6361 gtcaggaaaa aaggcgacag agtaatctgt cgcctttttt ctttgcttgc tttctgttag
     6421 attccgccgc aaatctatac tgaataaaac ggctaaaaga cgaaccatta tgaaaccatc
     6481 atcttcaaac tcacgcagca aaggtcatgc aaaagcgcgt cgaaaaacac gcgaggagct
     6541 ggatcaggaa gctcgtgatc gcaagcgtca gaaaaaacgt cgtggtcatg cgccgggcag
     6601 ccgcgcagcg ggcggaaaca ccacgtcagg cagcaaaggc cagaacgcac caaaagatcc
     6661 acgtattggc agtaaaaccc ctattccatt gggcgtggct gaaaaagtca ccaaacagca
     6721 caaaccgaag agtgagaaac ctatgctttc accgcaggcg gagttggaat tactggaaac
     6781 ggatgagcgt ctggatgcgc tgctggaacg tctggaagca ggcgaaaccc tgagcgccga
     6841 agagcaatcc tgggtggatg ctaaactgga tcgtattgat gagttgatgc agaaactcgg
     6901 cctctcttat gacgatgacg aagaagagga agaagacgag aagcaagaag acatgatgcg
     6961 tctgctgcgg ggtaactaac ggattgccac cgtgggcctt cccgttctgc tgataaccct
     7021 tccggttata tgttatctgg tgtggttatt cgttaaacta cagcggttgt cgcggcgaca
     7081 aaagtggctg cgcaaccggc tgatgacccg aaacgggcat ccgccggtac gccgtagccg
     7141 ccagagacgc catcggaagg agtgagcatg tctgtacagc aaatcgactg ggatctggcc
     7201 ctgatccaga aatataacta ttccgggcca cgatacacct cgtacccgac cgcgctggag
     7261 ttttcagaag acttcggcga acaggcgttt ttacaagccg tggcgcgcta tcctgagcgt
     7321 ccattatctc tctacgtaca tatcccgttc tgccataagc tttgttactt ctgcggttgc
     7381 aataagattg ttactcgcca gcagcacaag gccgatcagt atctggacgc gctggagcaa
     7441 gaaatcgtcc atcgtgcacc gctgttcgcc gggcgtcacg tcagccaatt gcactggggc
     7501 ggcggaacgc cgacgtatct gaataaagcg caaatcagcc gcctgatgaa gctgctgcgc
     7561 gaaaacttcc agttcaatgc cgatgcggag atttcgatcg aagtcgatcc gcgggaaatc
     7621 gaactggatg tactcgatca tttacgcgcc gaaggcttta atcgcctgag catgggcgtg
     7681 caggacttca acaaagaagt gcaacgtctg gttaaccgcg agcaggatga agagttcatc
     7741 tttgcactgc ttaaccatgc gcgtgagatt ggttttacct ccaccaacat cgacctgatt
     7801 tacggcctgc cgaaacagac gccggagagt ttcgccttta ccctgaaacg tgtggcggag
     7861 ctgaaccccg atcgtctgag tgtctttaac tacgcgcatc tgccgaccat ttttgctgct
     7921 cagcgcaaaa tcaaagatgc tgacctgccg agtccgcagc aaaaactcga tatcctgcag
     7981 gaaaccatcg ccttcctgac gcaatcgggc tatcagttta tcggtatgga tcactttgcc
     8041 cgtccggatg acgagctggc ggtggcccag cgtgaaggcg tgctgcatcg taacttccag
     8101 ggctacacca ctcagggcga taccgatctg ctggggatgg gcgtttccgc catcagcatg
     8161 attggcgact gctacgcgca gaaccagaaa gagttgaagc agtactatca gcaagtggat
     8221 gaacaaggca atgctctgtg gcgtggtatt gcgctaacgc gtgatgactg tattcgccgc
     8281 gatgtgatta agtcgctcat ctgcaacttc cgtctggatt acgcccctat tgagaaacag
     8341 tgggatttgc acttcgctga ttactttgcg gaagatctca agctgctcgc cccgttagca
     8401 aaagatgggc tggtggatgt ggatgagaag ggaatacagg tgacggcgaa aggtcgcttg
     8461 ctgatccgca acatttgcat gtgctttgat acctatctgc gccagaaagc gcggatgcag
     8521 cagttctcac gggtgattta aatagtgaat ggcgcttcgt ttacaaagta gaaaacgaaa
     8581 gcgccatcaa tgtgctgaga acaagattgc ctgatgcgct acgcttatca ggcctacaaa
     8641 acctgttgaa aatattgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga
     8701 acaaagagca cttcatcagc aatctgaaac tcaactaaac agcccaatca ttgcggcaca
     8761 cagcacggca gcaacggcag tttgcggtgt gtggctgtca actgcgccac tcgataccag
     8821 attaattatt gattccagca taatgactct ccccgttttc cgggcaagat catactgaac
     8881 ttatcggaac agtaaagcgt aaaataccag caatttgcgc tcaataatca atctttacac
     8941 acaagctgtg aatcactcca tccccagctc ttttaactta cgcgtcaggg tgttgcggcc
     9001 ccagccgagt agccgcgccg cttcctgttt atgcccctgc gtatgtcgta acgcggtcgt
     9061 cagtaacgtc cgctccagct ctggctgcgc ttcggaaagc agattttgat gaccggaacg
     9121 cagcgctctg tctgcccact gcgcaagaag cgttgcccag ctgtccggtt gcatttgcga
     9181 agtactctcc gcaaccgttg attcaaacag ttcgccgggc aaatcctgaa tcaacacttc
     9241 ctgcccggcg gccatcaccg ttagccagcg gcaggtgttt tccagctggc gcacgttgcc
     9301 tggccacgcc agacgcgtca gagcagcttc ggtttccgga tgcagtaact tcgcttctac
     9361 gcccagttcg cgcgcggcaa cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg
     9421 acgttcgcgc agcggcggca gatgaacgcg gataacgttc aggcggtgga acagatcctc
     9481 acggaactta ccttcctgca ctcgctgttc gagattctgg tgagtggcag cgataatacg
     9541 cacatccact ttcaccggcg catagccgcc aacgcggtaa aactgaccgt ctgccagcac
     9601 gcgcagcaaa cgcgtctgca catccagcgg catatcacca atttcgtcga ggaataatgt
     9661 accgccatcg gcctgttcaa aacgcccctg acgaatggta ttcgcgccag taaacgcgcc
     9721 tttctcgtgg ccaaacagtt ctgattcgat caaatctttt gggatagctg ccatattcag
     9781 cgcgataaac ggcgctttgg cgcgcggact gtggcgatgc agggcatgag cgaccagttc
     9841 tttaccggtg ccggattcgc cgttaatcag cacgctaata gaagaacgcg aaagccgacc
     9901 gataatacgg aacacgtcct gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc
     9961 gttaagctga atattacgcg gctgctgctg ttcctggtaa tgactgatag cgcgctcaac
    10021 cagcgccact gcttcgtcga tatcaaacgg tttgggcaga taatcaaacg ccccttgttg
    10081 ataggcgctg acggcagcat ccagatcgga atgtgcggtc ataatgatga ccggaagcat
    10141 tggatggcgc tgtttaatct gcttgagcag cgccagcccg tccattcccg gcatacggat
    10201 atctgaaagc agcacatccg gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt
    10261 ctcaaatgtc gtacaggtta aacccgctcc agcgagcgca cgttcaagca cccaacggat
    10321 ggaactatcg tcatcgacta cccagactat ccctcgttgc ataaacgtca cctttatttc
    10381 ctgataggca ggtaaaccga gaactcggta tgacctggcc aactggtaaa ttcaattttg
    10441 cctgaatgct gatcaatcaa attacgggcg atggataagc caagcccggt gccaccttcg
    10501 cggccgctga ccatcgggta aaacagcgta tcctgcaaat gaggcggaat gcccggcccg
    10561 ttatcttcca catcaatccg cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga
    10621 aacgcggtgc gggtacgcag aatgatttca ccgccttccg gccccagcgc ctgtagcgca
    10681 ttgcgcacaa tattcagcaa gacctgttca atttgatccg ggtcgtgcgc cagttccggc
    10741 aggctggggt cgtaatcacg aatcaaccgc acgttgtccg gcagttccat cgacaccagc
    10801 gtcaccacgc gttcagctac tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc
    10861 ggccccaaca gacggtcgac cagatttcgc agccggtccg cctgttcgat aatcactttg
    10921 gtatattcga gtagtgatgg gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt
    10981 aaacctccaa gcggattttt aatctcatgt gccaggccgc gcactaaatc acgggcagca
    11041 acctgctggg cgtgctgtag ctgttcctga cttaagcggc gctggttatc catcggagcc
    11101 atctccagca ggatcatgcc gtccggcata cgctgggccg tcacagaaag gatatgcgag
    11161 cgcccgtcga tgaccagcgt cacttcgtta tcggtaaaac cttgccccgc ctccagactt
    11221 tcttgcatca gctcgatatt taatgagaag tagctcaaca gttccggtaa cggtgtacca
    11281 aacaatttgc gggagctttg ggcgagcagt tgttgcgcgg cggggttggc gtaatggatc
    11341 gccagattgt catcaattaa caaaatactg ttaatcagcg agttgaggat ctgcccagca
    11401 tcgggctgcg tgcctgttgc cataaagcag tctcctgaac aggttgcacc attttagtgc
    11461 attatagctt tttacggata aaaagcgcga agcatcagag aattgacgga gaaaaaagcc
    11521 catgcagaga tgggctgaaa gtttccacgg caactaaatc ccggcgttgt tgcgccgggt
    11581 agtacaactt caactattag acgctgtagt acagctcaaa ctctaccgga tgcggagtca
    11641 tacgcacgcg gtcatcttct tcgcgacgta gagcgatgta cgcatcaatt gcttcgtcag
    11701 tgaacacgcc accggctttc aggaactcgc ggtccagatc cagttcgttc agtgcttctt
    11761 ccagagagcc tgcaacctgt gggatctctt tcgcttcttc tggcggcagg tcatacaggt
    11821 ttttgtccat ggcttcgccc ggatggatct tgttcttgat accatcaaga ccggccatca
    11881 gcagggcagc aaagcacagg tacgggttag ctgccggatc cgggaaacgt acttcgatac
    11941 gacgtgcttt cggagaagaa accaccggaa tacggataga cgcagaacgg ttacgcgcag
    12001 agtaagccag cattaccggt gcttcatagc ccgggaccag acgcttataa gagttggtgg
    12061 tcgggtttgc cagggcgtta atcgctttag cgtgtttgat tacgccgcca atgtagtaca
    12121 gcgcctgctc agacagacct gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag
    12181 acagagacat gtggcagtgc ataccggagc cgttatcacc gaacatcggt tttggcataa
    12241 aggtcgcggt tttaccgaag cggtgcgcta cgttgtgcac aacatatttg tagatctgaa
    12301 tttcgtcagc ttttttggtc atagtattga agcgggtagc cacttcgttc tgaccagcag
    12361 tcgctacttc gtggtgatgg gcttcaacca ccagacccat ctgttccatc accagacaca
    12421 tttcagaacg aatatcctga gccgagtcta ccggtggaac cgggaagtaa ccgcctttca
    12481 ctgccggacg gtgacctttg ttaccacctt cgtattgggt ggaggagttc catgcgcctt
    12541 cgatatcgtc gatagcaacg tgggaaccgg agatagatga tccgaaacgg atgtcatcga
    12601 acaggaagaa ttcaggttct ggcccgaaca gtacggtgtc ggcaatgcca gtggaacgca
    12661 ggtaatcttc ggcgcgcttc gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc
    12721 caggttcaag gatgtcgcaa cggataatca gggtggagtc ggcgaagaac gggtcaatca
    12781 ctgcggtgga tgcgtctggc atcagcacca tgtcggactc gttaatgcct ttccagccgc
    12841 caatcgagga gccgtcaaac attttgcctt cttcgaagaa ttcagcattc acctgatgag
    12901 cagggatagt gacgtgctgt tctttacctt tagtatcggt gaagcgcaaa tcaacaaact
    12961 tcacttcgtg ctcgttcagc atcgtcagta cgtgttcagc ggacatactt taactctcct
    13021 ggattggtca tggtcgtcgt ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa
    13081 aaaagataaa gcgaaatctg tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg
    13141 caccatcgtg caaaagggct gcaccacgat gtgaatgttg caccaatata gtgcttcaat
    13201 ggaaacatta agcaccatgt tggtgcaatg acctttggat aacccttttt atgctccgtg
    13261 aaagcgatca caaagggact ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt
    13321 tgtagtgcga ttaatccgtg tacaataacg cgctatttct aatgcctgag gcaaagttgt
    13381 gatcgaaaaa ttgcgtaata tcgccatcat cgcgcacgta gaccatggta aaaccaccct
    13441 ggtagacaag ctgctccaac aatccggtac gttcgactct cgtgccgaaa cccaagagcg
    13501 cgtgatggac tccaacgatt tggagaaaga gcgtgggatt accatcctcg cgaaaaacac
    13561 cgctatcaaa tggaatgatt accgtatcaa catcgttgat accccggggc acgccgactt
    13621 cggtggtgaa gttgaacgtg taatgtccat ggtagactca gtgctgctgg tggttgacgc
    13681 atttgacggc ccgatgccgc aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct
    13741 gaagccgatt gttgttatca acaaagttga ccgccctggc gcgcgtcctg attgggttgt
    13801 ggatcaggta ttcgatctgt tcgttaacct cgacgcgacc gacgagcagc tggacttccc
    13861 gatcgtttac gcttctgcgc tgaacggtat cgcgggtctg gaccacgaag atatggcgga
    13921 agacatgacc ccgctgtacc aggcgattgt tgaccacgtt cctgcgccgg acgttgacct
    13981 tgacggtccg ttccagatgc agatttctca gctcgattac aacagctatg ttggcgttat
    14041 cggcattggc cgcatcaagc gcggtaaagt gaagccgaac cagcaggtca ctatcatcga
    14101 tagcgaaggc aaaacccgca acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga
    14161 acgtatcgaa accgatctgg cggaagctgg cgatatcgtg gcgatcacgg gccttggcga
    14221 actgaacatt tctgacaccg tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc
    14281 cgttgatgag ccgaccgttt ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa
    14341 agaaggtaag ttcgtaacgt ctcgtcagat cctggatcgt ctgaacaaag aactggtaca
    14401 caacgttgcg ctgcgcgtag aagaaaccga agacgccgat gcgttccgcg tttctggtcg
    14461 tggcgaactg cacctgtctg ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc
    14521 ggtatcccgt ccaaaagtaa tcttccgtga aatcgacggt cgtaaacaag agccgtatga
    14581 aaacgtgact ctggacgttg aagaacagca tcagggttct gtaatgcagg cgctgggcga
    14641 acgtaaaggc gacctgaaaa acatgaatcc agacggtaaa ggccgcgtac gtctcgacta
    14701 cgtgatccca agccgtggtc tgattggctt ccgttctgag ttcatgacca tgacttccgg
    14761 tactggtctg ctgtactcca ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg
    14821 tcagcgtcag aacggcgtac tgatctctaa cggtcagggt aaagcggtcg cgttcgcact
    14881 gttcggtctg caggaccgcg gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg
    14941 tcagattatc ggtattcaca gccgctctaa cgacctgact gtaaactgcc tgaccggtaa
    15001 gaaactgacc aacatgcgtg cttccggtac tgacgaagct gttgttctgg ttccgcctat
    15061 ccgcatgact ctggaacaag ctctggagtt catcgatgat gacgaactgg tagaagtgac
    15121 tccgacctct atccgtattc gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa
    15181 ccgcgcaccg aaagacgatt aatttcgttt tttagttata aaaaacct
//
LOCUS       DAJTVJ010000100        15087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000100 DAJTVJ010000000
VERSION     DAJTVJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.100"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /locus_tag="OO448_003061"
     CDS             <1..781
                     /locus_tag="OO448_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338210.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="OO448_003062"
     CDS             complement(984..1337)
                     /locus_tag="OO448_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6338211.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OO448_003063"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OO448_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6338212.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="OO448_003064"
     CDS             complement(3165..3458)
                     /locus_tag="OO448_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6338213.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="OO448_003065"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="OO448_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6338214.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OO448_003066"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OO448_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6338215.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="OO448_003067"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="OO448_003067"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6338216.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="OO448_003068"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="OO448_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6338217.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="OO448_003069"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="OO448_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6338218.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="OO448_003070"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="OO448_003070"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6338219.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="OO448_003071"
     CDS             10838..11413
                     /locus_tag="OO448_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6338220.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="OO448_003072"
     tRNA            11520..11595
                     /locus_tag="OO448_003072"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="OO448_003073"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="OO448_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15087
                     /gene="cadB"
                     /locus_tag="OO448_003074"
     CDS             14114..>15087
                     /gene="cadB"
                     /locus_tag="OO448_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR6338221.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLL"
BASE COUNT         3708 a         3765 c         3617 g         3997 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgctgct
//
LOCUS       DAJTVJ010000101        14998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000101 DAJTVJ010000000
VERSION     DAJTVJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.101"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..659
                     /locus_tag="OO448_003075"
     CDS             <1..659
                     /locus_tag="OO448_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6338222.1"
                     /translation="FIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITI
                     RRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRS
                     TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWS
                     LAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG
                     "
     gene            695..1381
                     /gene="mlrA"
                     /locus_tag="OO448_003076"
     CDS             695..1381
                     /gene="mlrA"
                     /locus_tag="OO448_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR6338223.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1441..1548
                     /locus_tag="OO448_003077"
     CDS             1441..1548
                     /locus_tag="OO448_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6338224.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1529..2260)
                     /gene="yehW"
                     /locus_tag="OO448_003078"
     CDS             complement(1529..2260)
                     /gene="yehW"
                     /locus_tag="OO448_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6338225.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2265..3191)
                     /gene="yehX"
                     /locus_tag="OO448_003079"
     CDS             complement(2265..3191)
                     /gene="yehX"
                     /locus_tag="OO448_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6338226.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3184..4341)
                     /gene="yehY"
                     /locus_tag="OO448_003080"
     CDS             complement(3184..4341)
                     /gene="yehY"
                     /locus_tag="OO448_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6338227.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4348..5265)
                     /gene="osmF"
                     /locus_tag="OO448_003081"
     CDS             complement(4348..5265)
                     /gene="osmF"
                     /locus_tag="OO448_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6338228.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5517..7814)
                     /gene="bglX"
                     /locus_tag="OO448_003082"
     CDS             complement(5517..7814)
                     /gene="bglX"
                     /locus_tag="OO448_003082"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6338229.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8010..9725
                     /gene="dld"
                     /locus_tag="OO448_003083"
     CDS             8010..9725
                     /gene="dld"
                     /locus_tag="OO448_003083"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6338230.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9764..10696)
                     /gene="pbpG"
                     /locus_tag="OO448_003084"
     CDS             complement(9764..10696)
                     /gene="pbpG"
                     /locus_tag="OO448_003084"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6338231.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10870..11457)
                     /locus_tag="OO448_003085"
     CDS             complement(10870..11457)
                     /locus_tag="OO448_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6338232.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11627..12205
                     /locus_tag="OO448_003086"
     CDS             11627..12205
                     /locus_tag="OO448_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6338233.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12335..13096)
                     /locus_tag="OO448_003087"
     CDS             complement(12335..13096)
                     /locus_tag="OO448_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6338234.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13149..14585)
                     /gene="mdtQ"
                     /locus_tag="OO448_003088"
     CDS             complement(13149..14585)
                     /gene="mdtQ"
                     /locus_tag="OO448_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6338235.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            14809..14892
                     /gene="yohP"
                     /locus_tag="OO448_003089"
     CDS             14809..14892
                     /gene="yohP"
                     /locus_tag="OO448_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6338236.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3623 a         4109 c         3776 g         3490 t
ORIGIN      
        1 gttttattga ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca
       61 cgggtattcg tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga
      121 ggactataac catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg
      181 atgctggtac cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc
      241 aagcggaaat gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg
      301 ggagaagtac tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc
      361 agttttccgg accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa
      421 aacgcgcagg tctgagacac agaaaatcgt accaatccag gcatacttat gcatgctggt
      481 cacttgccgc tggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac
      541 aaatggtatt caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac
      601 tccttaacaa aagactatct gaaagtgtcc catgtatgcc ccataagaaa gtggggtaaa
      661 ataaaaactt gtaaaatcag ttagtttacc cttaatccct gtcacgttac gcgcgtggca
      721 gaggcgttac gggttgctga aaccgcaacg gacagacggc ggtcatcgac tgttcaacga
      781 tgccgatatt gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag
      841 caaagttaaa atgctgctca gtaatgaaaa tgttgatgtg cagaacggct ggcgcgatca
      901 gcaagaaaca ttactgacct acctgcaaag cggcaatctg catagcctgc gaacgtggat
      961 caaagagcgc ggtcaggatt accccgccca gacactcacc acacatctgt ttattcctct
     1021 gcgccgacgg cttcagtgcc aacaaccgac tctccaggcg ctgctggcga tcctcgacgg
     1081 cgtactgatc aactacatcg ccatttgtct ggcttcggca cgtaaaaaac agggtaaaga
     1141 tgcgctggtg gttggctgga atattcagga taccacccgt ctgtggctgg agggctggat
     1201 tgccagtcaa caaggatggc gcattgatgt cctcgcccac tcgctcaatc aactacgccc
     1261 tgaactattc gaaggccgta cattgctggt gtggtgcggt gaaaatcgaa cctccgccca
     1321 acagcagcaa ctcaccagtt ggcaagaaca aggccatgat attttcccac tcggcattta
     1381 atgattcgtt aacaaatgcg ctttactgta caatcctttc gttaacataa ggagtgcaat
     1441 atgcgcatag ctaaaattgg ggtcatcgcc ctgttcctgt ttatggcgtt aggcggaatt
     1501 ggtggcgtca tgctcgcagg ttataccttt attttgcgtg ctggctaagc gcctgcacca
     1561 gcctttcaaa caggcggtct gcgatgatcg ccgccagtgc caccagtaac gccccctgga
     1621 tcacatacgc ggtattaaat ccgctaagcc cgatgatgat gggcgtaccc agcgtgctgg
     1681 cccctaccgt tgaggcgatc gtcgccgtac caatgttgat aatcaccgaa gttcgcacgc
     1741 ccgccagaat caccggagcc gccagcggta gctcgacctt acgcagtcgc tgaccacgac
     1801 tcattcccat acctttcgca acttctgtca cgctggcatc aatcgctccc agcccggcaa
     1861 gtgtcgcctg caggacgggc agcacaccgt aaaggatcaa ggcgataatc gctggttgca
     1921 gaccaaagcc gatcaccgga acggcaatcg ccagcactgc gacaggcgga aaagtctgtc
     1981 caacggcggc aatagtttcc accagtgggc gaaattccgc gccccacggg cgagtgacag
     2041 caattccggc accagtgcca atgatcaccg caaacaaact cgaaattccc accagccaga
     2101 aatgagccag tgccagagct gcaaaacttt cttgctgata aacgggtcgt ggcagttgtg
     2161 ggaacaaggc agcaaacagc ggctggctgt aaggcagcca gaaaatcagt gccacaaaca
     2221 aagcaatgag ccagaacagc ggatcgcgca acatcttcat acgcttacgc ctcctccagc
     2281 agatcctgaa aatgcagcgt gccgcaaggc tggccctgcg tgttcaccac cggcagcacc
     2341 tcgcatcccc gcgcgacaaa cagggagagc gcatcgcgta gcgtcatctc ttctgccagt
     2401 gcctcacctt ctgcccgttc ttcgcgacgc acgtaatccg ccacactacg taacgaaagc
     2461 aggcgcacac ccagttcgct gcgcccaaaa aactggcgga caaaatcgtt tgccggacga
     2521 gtcagcatcg tcagcggatt gccctgctgc accacttcac cgtgatccat caataccaga
     2581 tgttctgcca gccgtagcgc ctcatcaata tcatgagtga ccagcacaat ggtacgcccc
     2641 agcaaacggt gaatgcgcgt catctcttgt tgcaacgcgc cgcgcgttac cgggtccagt
     2701 gcgccaaaag gttcatccat cagtaagact tgcggatcgg cagccagtgc gcgcgccact
     2761 cccacacgtt gctgctgacc accggaaagc tgatgcggat aacgctcacg taaatttgac
     2821 tccagcccca gtagcgccat taattcgtcg atacgatcgt caatcctcgc ccgcgaccat
     2881 ttttgtaatt gcggcacggt agcgatgttt tgcgccacgc tccagtgggg gaacaggcca
     2941 atagattgaa tggcatagcc catccggcgg cgcaactcca gtactggcag cgagcgaatt
     3001 tcttctccgg caaagcggat cactccgctg tcatgctcca ccaggcggtt aatcattttc
     3061 agggtggtgg atttgccgga gccagatgtg ccaatcagca ccgaaaaact cccttcctga
     3121 aaattgagat tgagatcgtt aacggctttt tgtgcgccga acagtttgct gacatggcca
     3181 aattcaatca ttacgtttca ccttcagcag tgcgataagc aaatcgaaca gcgcgtcggt
     3241 aagcaccgcc agaacaatta ccgggatcac ccccagcaac actaaatcaa tggcgctgct
     3301 tagcagcccc tggaaaacca gcgcaccaaa accgcctgcg ccgattaacg ccgcaatcac
     3361 cgccatacct acagtttgca ccatcaccac ccgcaggctg cgcagaaata ccggtaacgc
     3421 cagtggtaac tgaacatgca ggaatcgctg cgccccgctc atgcccatcg ctctggcgct
     3481 ctccagcaca tcgcgcggga tctggttcaa accgactacc acgccgcgca ccagcggcag
     3541 caaggcatag agcaccagcg caatcagtgc gggtgtcatt ccggttcctg ctatgccgag
     3601 cgtccccagc cacggaaagg ccgtaaccag cgcggcaagc ggcgcaatca acaggccaaa
     3661 gagcgccacc gaaggcacgg tctgaatgac attgagcaga gaaaaaattg ccccctgccg
     3721 agcagtggaa aagtagcacc agatgcccaa cggcacacca atcactaacg caggcagcac
     3781 cgcaccaaac agcaacgtca gatgttgtgc cagcgcgtcg tcaaacacat cctgacggtt
     3841 ggcgtattct ttcattagtg agagatcgtt aagcgtgccg gagtacagca accacagcgg
     3901 aataatggca atctgcatat gcaacaacca gcgccacagc ggatgcgtgg agattcggcg
     3961 gatggcatca ctacaggcca gcaatgccag cgccgcggcc agccagaaac cactgccgag
     4021 gctggtacgc gccagcgcac tgccattttg cgccagttgg gtcgccgcct ttcctgctcc
     4081 ccacaccagc aatacgaaga cgaattgcgc cagaatgagt gcaaaaatgc tgcctttttt
     4141 agcgggaata aaacaggctg tcagccaggc gcaaccaacg cccaccagca tccagatcgt
     4201 ttgcggccac agttgccaga gatgacgccc ttcacccgag actaaacgat taggcgcgta
     4261 actgataaac ggcagcgccg ctgcgattgc cgtcagcaac agcagcagcg ccagaacagg
     4321 attaatacgt agataagtca cgggaaatta cttcgtccac cctttttgtt tcaggtagtc
     4381 ggcagccact tttttggcat ccagtccttc cactgcaatg ctggcattca gttgctgcaa
     4441 tgtttttgca tcgaggctgg cgaagactgg ctgtagccac tgtgccattt gcggatactc
     4501 tttcagcacc gactcacgca ccactggtgc aggcgcgtag ataggttgca cgcctttcgg
     4561 atcgcttaag gtttgcagcc ccagcgccgc gaccggaccg tcagtgccgt aagccattgc
     4621 ggcattaacg ccagaagttt gctgggcagc ggctttaatc gttaccgccg tgtcgccgcc
     4681 agccagtgac agtaactgat cctgatcgag tttaaagtcg taggcttttt caaacgcggg
     4741 taacgcatcg gcgcgttcga taaactctgc cgaggctgcc agtttgaagg tgccgccctc
     4801 tttcagataa cgactcaggt cggcaagcga agtgagtttg tttttctctg ccacatcctg
     4861 acgcacggcg atggtccagg tgttattcgc tggtgcgggc gtcagccaga ttaacttgtt
     4921 ttgctctgca tcgagttttt tgactttctc gtaaccttgc tgcgcgtttt tccatgccgc
     4981 atcgttttca tctttaaaga agaaagcgcc attaccggta tattccggat agatatccag
     5041 ttcaccggaa gtaatcgccc cgcgcaccac aggagtcgtt ccgagttgca ctttattcac
     5101 cgtcggtacg ccgtggcttt ccagcacctg caagatgatg ttgccgagta gcgcgccttc
     5161 ggtatcgatt tttgaaccga ctttaacggg ggaagccgcc tgtagcggca ggctcacggc
     5221 tgccaacata accagtgaac ctgcccagac ctttgagagt ggcatgatgc tttcctcatt
     5281 cttttactgt tgttttcagc gaattaagag aaaagcatag ttgataatga cagagttagc
     5341 ctgagcggaa ggattcagtt gcagaatcag ataaatactt aaagggcata ttttgcccgg
     5401 cgtttcttgc cggatgcggc gtaaacgcct tatccggcct acaaaggtcg aaaattcagc
     5461 aagttaagat atgaacatca ggcaaagggc gcaaacgtcg cgcccttact aaagcattac
     5521 agcaactcaa actcgccttt cttaacgcgt gcggaatcag tgccgataaa gacattgaac
     5581 ttgccaggtt cggcgtcata tttcatctgt tgattccaga acttcagcgc ctcgatatca
     5641 atcgggaagc tgacagtctg agtttcgccc ggtttcaggg tgattttctc aaagcctttc
     5701 agctgcttca ctggacggct catggaagcc gtcacatcct gcaagtacat ctgtactacc
     5761 gtggcccctt cgcgtttacc ggtgttcgtc acctgcacgc tggcggtcac tttgccgtca
     5821 cgcttcatgg tcggcgcaga aagtttcaca tcagagacgg tgaaagtggt atagctcaga
     5881 ccatagccga acggataaag cgccccgtta gcttcatcaa aataacgcga agtgtatttg
     5941 ttcggcttgt cggcattata cggacgaccg gtattcagat ggctgtagta caccgggatc
     6001 tgcccgacag aacgcgggaa ggacattggc agcttgccgg acgggttgta atcgccaaac
     6061 aatacatcgg caattgcatt accgccttca gtccccgcaa accaggtttc cagaatcgca
     6121 tcagcctgct gatcttcttt caccagcgcc agcggacgcc cgttcatcag caccagcacc
     6181 agcggtttac cggtggcttt cagcgccgta atcaagtcac gttggctttg cggaatagtg
     6241 atatcggtac ggctggaggc ctcgtgcgcc atcccctgtg cttcaccgac cacagccacc
     6301 accacatcag attgtttcgc cgtctgcacc gcttcatcaa tcatctcttg cggcgagcgc
     6361 ggatcgactt tgaccgcttc ttcatactga ttcaggaaat cgataatgcc tttgtcactg
     6421 gtaacgttcg cccctttggc atacagcact ttaccgtttt caccgacggc atttttaatc
     6481 ccggtcagta cggtcacgga ttgatcggca acaccggctg cggaccagct gcccatcacg
     6541 tcacgtttac tgtccgccag tggcccaacc accgcaatgg tggccgattt tttcagcggt
     6601 aacgtttcga gacggttttt cagcaacacc aggctttcgc gcgccacttc acgcgcttct
     6661 ttacggtgca ggcggctttc ggcattggta tccaccgggt cagactcttt cggccccaaa
     6721 tggctgtatg ggtcgttaaa caatcccata tcatatttaa cgttcagtac atggcgggca
     6781 gcatcgtcca gctcttccat cgtcactttg ccggatttga tcaacccagg cagatacttc
     6841 gagtaatact cgtcgctcat actcatgttg attccggatt tcagcgccac gcgcaccgca
     6901 tcttccgggt ctgccgccgt gccatgttta atcagctctt tgattgcacc gtgatcggaa
     6961 acggtgatgc ctttaaagcc ccactggtcg cgcagaacat ctttcagcag ccaggaatcg
     7021 gaggtggctg gcgtgccgtt cagcgagttc agcgccacca tcaccgcgcc gctgcctgcg
     7081 tccagccccg ctttgtacgg cggcatataa tcattaaaca ggcgctgcgg actcatatcg
     7141 acggtgttgt actctttacc gccttctacc gcgccgtatg cggcaaagtg tttgacgctg
     7201 gtcatcaccg agtagcgatc tgccgggctt ttaccctgca tcgcttccac catggtttta
     7261 cccattgttg aggtgagata cgtatcttcg ccaaaacctt cggaagcacg tccccagcgc
     7321 ggatcgcgcg agacatcgac catcggtgcc caggtcatat tcaggccatc atctgccgct
     7381 tcataagcag agacacgtcc gaccgttttc actgcatcga ggttaaaaga cgaggccaga
     7441 ccgaggctaa tcgggaacac cgtgcgctga ccgtgcagca cgtcgtaagc aaagaaaaga
     7501 ggaattttca ggcggcttaa ttccatcacc tgatcctgca tggcgcggat atcctgacgg
     7561 gttacggtgt tgaaaatcgc cccaacctga ccgtctttga tcatctcgcg gatcgcctct
     7621 ttcgggttat ccggaccgac gctgattaag cgcagctgac caattttctc atcaactgtc
     7681 attttcttaa gcagttcggt gacgaacgca tcccgcgctt cgggcgttaa tggatggttg
     7741 ccgaataaat catccgccag tgctggctgc agggccagac tcaccgcgat tcctactgaa
     7801 catagccatt tcatatggat ttttcttcct ctgttgcccg acgaggcagc gaaaatggtg
     7861 caaaaaccgt agtttgccat aagcatgatg gagagagaaa aagaatgctc agtttattgt
     7921 ctgaattttc aaaatattca ctcgctgaat tgtcatacaa ggcgctattc tagtttgtga
     7981 tattttttcg ccaccacaag gagtggaaaa tgtcttccat gacaacaact gataataaag
     8041 cctttttgaa tgaacttgcc cgtctggtcg gtcattcaca cctgctcacc gatcccgcaa
     8101 aaacggcccg ctatcgcaag ggcttccgtt ctggtcaggg cgacgcgctg gctgtcgttt
     8161 tccctggctc actactggaa ttgtggcggg tgctgaaagc ctgtgtcacc gccgacaaaa
     8221 ttattctgat gcaggctgcc aatacaggcc tgaccgaagg atcgacgcca aacggtaacg
     8281 actatgatcg cgatatcgtg atcatcagca ccctgcgtct cgacaagctg cacgttcttg
     8341 gcaagggcga acaggtgctg gcctatccgg gcaccacgct ctattcgctg gaaaaagccc
     8401 tcaaaccgct gggacgcgaa ccgcactcag tgattggatc atcgtgtata ggcgcatcgg
     8461 tcatcggcgg tatttgtaac aactccggcg gctcgctggt gcaacgtggc ccggcgtata
     8521 ccgaaatgtc gttattcgcg cgtataaatg aagacggcaa actgacgctg gtgaaccatc
     8581 tggggattga tctgggcgaa acgccggagc agatccttag caagctggat gatgatcgca
     8641 tcaaagatga cgatgtgcgt cacgatggtc gtcacgccca cgattatgac tatgtccacc
     8701 gcgttcgtga tattgaagcc gacacgcccg cacgttataa cgccgatcct gatcggttat
     8761 ttgaatcttc tggttgcgcc gggaagctgg cggtctttgc tgtacgtcta gataccttcg
     8821 aagcggaaaa aaatcagcag gtgttttata tcggcaccaa ccagccggaa gtgctgaccg
     8881 aaatccgccg tcatattctg gctaacttcg aaaatctgcc ggttgccggg gaatatatac
     8941 accgggatat ctacgatatt gcggaaaaat acggcaaaga caccttcctg atgattgata
     9001 agttaggcac cgacaagatg ccgttcttct ttaatctcaa gggacgcacc gatgcgatgc
     9061 tggagaaagt gaaattcttc cgtccgcatt ttactgaccg tgcgatgcaa aaattcggtc
     9121 acctgttccc cagccattta ccgccgcgca tgaaaaactg gcgcgataaa tacgagcatc
     9181 atctgctgtt aaaaatggcg ggcgatggcg tgggcgaagc caaatcgtgg ctggtggatt
     9241 atttcaaaca ggccgaaggc gatttctttg tctgtacgcc ggaggaaggc agcaaagcgt
     9301 ttttacaccg tttcgccgct gcgggcgcag caattcgtta tcaggcggtg cattccgatg
     9361 aagtcgaaga cattctggcg ttggatatcg ctctgcggcg taacgacacc gagtggtatg
     9421 agcatttacc gccggagatc gacagccagc tggtgcacaa gctctattac ggccatttta
     9481 tgtgctatgt cttccatcag gattacatag tgaaaaaagg cgtggatgtg catgcgttga
     9541 aagaacagat gctggaactg ctacagcagc gtggcgcgca ataccctgcc gagcataacg
     9601 tcggtcattt gtataaagca ccggagacgt tgcagaagtt ttatcgcgag aacgatccga
     9661 ccaacagtat gaatccgggg atcggtaaaa ccagtaagcg gaaaaactgg caggaagtgg
     9721 agtaaaaatt acggatggca tagtatcgcc atccgtcatt cacttaatcg ttctgtgccg
     9781 tctgccccgc cgccgccatt tgggcggctt tttgtttttt atagctcaac gctgctgctg
     9841 gaacaggcat cactttacca gtttcaatcc aggtacgcag gcggctggca tcggcaaaat
     9901 gggtatattt gccaaacgca tccatcacta ccagcgctac cggtttatta ttgataaccg
     9961 tacgcatcac cagacaatgg cccgccgcat tggtaaaacc ggttttggtt aactgaatat
    10021 tccagttatc gcgatacacc agatggttag tattgcggaa cggcagcgta tacgtcgggt
    10081 tagagaaggt tgccatatct tcccgggtag tacttaactg cccgatcaac ggatattgtt
    10141 tgctggcaat gagcagtttg gttaagtcac gggcagttga aacgttatgc accgacaatc
    10201 cggtaggttc aacaaagcgc gtgttgttca ttccgagcga tttcgctttc gcattcattg
    10261 ccttaataaa ggctttgtaa ccaccgggat aatggtgtgc aaggcttgcc gccgcgcggt
    10321 tttctgaaga catcagcgcc agcaacagca tatctttacg gctgatttcg ctattcagtc
    10381 gtacgcgcga atagacccct ttcatctccg gcgtctggct gatatccact tttagttttt
    10441 catccagcgg cagtcgtgca tccagcacaa ccatcgcggt cattaatttg ctgatagacg
    10501 caatcggacg caccagatcc gggtggttcg agtagatcac tttgttggta ttcagatcaa
    10561 caatcatcgc gctaccggag gcaatttcag gttgtgaagc ggtggtagcg gctgccgttt
    10621 tcgcaacggc ctgcggtgcc aaaggcacag ccagcatcag ggccaggcta aataaagaaa
    10681 ctcgaaattt cggcatgatg agcattcaga tagtggttca cgcgcacagg ttgcgcaccg
    10741 ccggagtaag gatttactga ggctagcgac gccatcataa cgagcaaaaa gtgcgatcgt
    10801 caaaggagaa tcgtgaggaa atgctgcatt gctgacattt acgccagcaa tgcaacgtca
    10861 aagaactttc tagaacaaac gataaccgta gccccatagt ataacggtta gggcgagcag
    10921 cacttccagt accagcacgc caatcgccag cgtcgaacta gagaagctaa ggccttcctc
    10981 tttgttgata ttcaggaagc tcggaatacc aaggtaaagc aggtagccgg tgtaaaacag
    11041 cgccaccgtg ccgaccagcg cacacaacca gaccagtgga taaagcgcca ccagaccgct
    11101 taaaaacagc ggagttgcaa cgtagcccgc gaagaccatg cagtgcgcaa gtgacggacg
    11161 ctgcggataa ttacgcgcca tccaccagat gacccgcccc attaccgcaa ccccagccag
    11221 cataacgcca taaaacagga cagccagcgc cagtccggta aaccaggata acttcaggat
    11281 agtgccatcg ccaaaattcc agccaatctg tgtagtgcca atgaaggcgc aaatcaccgg
    11341 aatcgccgcc atcagcaaaa cgtggtgggt gtaatggtga gaaatcgttt cgttttcgcg
    11401 attaatcacc tgcatttcac gatcgggatg ggaaaacagt ccccagacat ggctcatacc
    11461 gcccccttgt tgtgagttca tgaacctgac agttcaagta taagtcagct tgtgattatt
    11521 ttttgttcgc tcatgtaatt tgcaccgtct gatgtacgtc ctttttcagc gtgaatgatt
    11581 tccagggtgt atgcttacag gcaaccaaag ggagacagac tggcctatgg atctcaatac
    11641 actcatctca caatatggtt atgccgcgct ggtgatcggt agcctggcgg aaggtgaaac
    11701 cgtgactttg ctgggaggcg ttgcggcaca tcaggggcta ttaaagttcc cgctggtggt
    11761 actttctgtg gcgcttggcg gcatgattgg cgaccaggtg ctttatctgt gcgggcggcg
    11821 gtttggcggc aagctgttac gccgtttctc gaaacatcag gataaaattg agcgggcgca
    11881 gaaacttatc caacgccatc cgtatctgtt tgtcattggt acgcgcttta tgtatggctt
    11941 tcgggtgatt ggcccgacgc tgattggtgc cagccagctg ccgccgaaaa tctttctgcc
    12001 gctgaatatt ctcggcgcat ttgcctgggc gttgattttt accactattg gttacgctgg
    12061 tggtcaggtg attgcgccgt ggttgcacaa tctcgaccag catttgaagc actgggtctg
    12121 gttgattctg gtcgtggttc tggtggtggg cgtgcgctgg tggctgaaac ggcgcgggaa
    12181 gaaaaagccg gatcatcagg cgtaaaacca ttgccctggg taaggcgttc gcgccgcatc
    12241 cgacatccgg tgcaccatgc ctgatgcgac gcttgtgcgt cttatcatgc ctaccaaaat
    12301 tttacccatt gaaataacca cgcacctcac tgcgctattc tgggttgaac tgcggattcg
    12361 ccaacataaa gccgccatcc actatcaacg actgcccggt ggtgtaattt gcgccctctg
    12421 agcacagcca cgctaccagg ctggcaatct catgcgttgc gccaaaacgc cgcagaggaa
    12481 tcgaaggctc cgcgtcgggc ttcacgtcgc tgtcatccat gccattcatt ggcgtggcga
    12541 tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct gaccagctcc agcgccatcg
    12601 ttttggttaa cccgccgagc gcatgtttag cggctgtgta agcgctggca tccggcagcg
    12661 gcgtatgttc atgtaccgac gtaatgttga tgatacgacc gccctgccct tgtttcacca
    12721 tctggcgggc cgcaatttgt gagcataaaa atgcaccatc gacatcaacg gtaaaaatct
    12781 tgcgccactc atcgaaagcc atatcaagaa acggcgcttt ggtcattgcg cccgcattat
    12841 tcaccagcac atcaatacgc cccagccgtt gaatgagttt ctccagcgcc tgcgcacctt
    12901 ctggcagttt accgagctcc agctgcacga tctccgcacg tacgccgtgg ctaactacct
    12961 cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca ggtaatgcca atatcaaacc
    13021 cctgctgcgc cagtaataac gcgcactctt tgccgatccc cgaatcggag gcggtaataa
    13081 tcgcaacctg tgccatcaag ttctccactt aacgctgaat aaacgttaag tatagaaggc
    13141 gcatatcatc agcgtttgta acccccgccc aacgcaccag tgagttgaat agaagcatcc
    13201 agccactgcc cttgcaataa caggccattg gcccgctcac gcagcgcggg gattctggct
    13261 tcgctgacgc gggaaccaac aatgatgccc gcgttaaagc gcgcctgcgc aagacctacc
    13321 acacgcaagg catcgcgctc aatttgcgcc tgatgctggt ttttctccgc aagtgtctga
    13381 acctgactgg ctgcccgcgc cacgtcattc accgcttcaa ccaccgcttt gttgtagctg
    13441 gcgatagaca agttgctttc ggcttttgcg atattgagat tggcgttaag acgaccacta
    13501 tcgaaaatgg gtagcgtcag gcctgccgta acgcccattt gctgcgcgga atgacggaac
    13561 agatcgctta agtgcaacgc atcctgttgc aggaaggcca tcaggttgat gtcaggataa
    13621 aatgccgctt ttgccgcatc aatggtgctt agcgatgact caacgtacca gtgcgccgcc
    13681 tgcaaatctg cccgccgggc cagtaaggag taccccagtt catcaggaag ctggcttgcc
    13741 actttcggca acgcgaccgg tttaagcttc aatgactttg tctggttatt tgtaagtgcg
    13801 cttaaccgtg cctcaataat tttcattttc cccgcgacat cgttgagctg ctgccgggtt
    13861 ttgctggcat taatatcggt ttccacacct tcaactgaag aagtaatccc gttctgatat
    13921 agctggcgat cggtcgcgat aatggtgttc tgctcttttt ctatttgctg caagaccgtg
    13981 tttaacgccg cctgggtttg ccactcccag tacaggcggg ctacgctgcc agccagcaat
    14041 tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg tacccaggcg ggcagtaacc
    14101 tccgcccgat tctttcccca gatatcgaga tgccagcccg ccgttaagcc aaaagtaccg
    14161 ttggtgtacc acgggccggt cgtacctgcg gccggatcgt tcagagcaaa cggccccatt
    14221 aagccttctg ccgacatttt ttgccgctcc atatccgccg aaaagtcgat ctgcggacca
    14281 tcctgagtgg caactgcctt cgcctgggct tcagctagct gaatgcgctg ttcagccacc
    14341 tgcatatccg gtgcgttctg tagtgcattg ttaattaatg aagtgagctg attatcgtga
    14401 tactccagcc accattggct gtctggccaa ccatttttca gcgccgtggg taatgcggtg
    14461 tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc gggtttcatg cataggcgca
    14521 cacccggcca gcatcagtaa cagcggaaaa caggcgatgg ctggataaaa ggaatcacga
    14581 ttcatggggg aataatcagg taagaaacgg tgcgcggaga ttaccgtgtg ttgcgatata
    14641 ttttttagtt tcgcgtggca atacatcagt agcaataaaa cgacataccc agaaaaatat
    14701 agactaagta aatgatatct tccgatttat cttaatcgtt tatggataac ggcaaagggc
    14761 ttcgtttttt cctatactta ttcagcactc acaaataaag gaacgccaat gaaaattata
    14821 ctctgggctg tattgattat tttcctgatt gggctactgg tggtgactgg cgtatttaag
    14881 atgatatttt aaaattaatt aatgtcatca ggtccgaaaa taacgagaat atttcagtct
    14941 ctcatcctgt tgcgctcctg tcatgtgcat tgcttcatat aatcactggc gcaaggag
//
LOCUS       DAJTVJ010000102        14965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000102 DAJTVJ010000000
VERSION     DAJTVJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.102"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OO448_003090"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OO448_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6338237.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OO448_003091"
     CDS             complement(1608..1931)
                     /locus_tag="OO448_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6338238.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO448_003092"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO448_003092"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6338239.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO448_003093"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO448_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6338240.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO448_003094"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO448_003094"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6338241.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO448_003095"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO448_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6338242.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OO448_003096"
     CDS             7531..8568
                     /locus_tag="OO448_003096"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6338243.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO448_003097"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO448_003097"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6338244.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OO448_003098"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OO448_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6338245.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO448_003099"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO448_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6338246.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OO448_003100"
     CDS             10739..11620
                     /locus_tag="OO448_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6338247.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO448_003101"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO448_003101"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6338248.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVHKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OO448_003102"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OO448_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6338249.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="OO448_003103"
     CDS             complement(14017..>14965)
                     /locus_tag="OO448_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR6338250.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3783 c         3922 g         3648 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttatgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAJTVJ010000103        14839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000103 DAJTVJ010000000
VERSION     DAJTVJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.103"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            102..191
                     /locus_tag="OO448_003104"
                     /pseudo
     CDS             102..191
                     /locus_tag="OO448_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(192..1250)
                     /gene="modC"
                     /locus_tag="OO448_003105"
     CDS             complement(192..1250)
                     /gene="modC"
                     /locus_tag="OO448_003105"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6338251.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1253..1942)
                     /gene="modB"
                     /locus_tag="OO448_003106"
     CDS             complement(1253..1942)
                     /gene="modB"
                     /locus_tag="OO448_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6338252.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(1942..2715)
                     /gene="modA"
                     /locus_tag="OO448_003107"
     CDS             complement(1942..2715)
                     /gene="modA"
                     /locus_tag="OO448_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6338253.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(2881..3030)
                     /gene="acrZ"
                     /locus_tag="OO448_003108"
     CDS             complement(2881..3030)
                     /gene="acrZ"
                     /locus_tag="OO448_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6338254.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3159..3947
                     /gene="modE"
                     /locus_tag="OO448_003109"
     CDS             3159..3947
                     /gene="modE"
                     /locus_tag="OO448_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6338255.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4015..5487
                     /gene="modF"
                     /locus_tag="OO448_003110"
     CDS             4015..5487
                     /gene="modF"
                     /locus_tag="OO448_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6338256.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            5748..6764
                     /gene="galE"
                     /locus_tag="OO448_003111"
     CDS             5748..6764
                     /gene="galE"
                     /locus_tag="OO448_003111"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6338257.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            6774..7820
                     /gene="galT"
                     /locus_tag="OO448_003112"
     CDS             6774..7820
                     /gene="galT"
                     /locus_tag="OO448_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6338258.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            7824..8972
                     /gene="galK"
                     /locus_tag="OO448_003113"
     CDS             7824..8972
                     /gene="galK"
                     /locus_tag="OO448_003113"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6338259.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            8966..10006
                     /gene="galM"
                     /locus_tag="OO448_003114"
     CDS             8966..10006
                     /gene="galM"
                     /locus_tag="OO448_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6338260.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10209..10961
                     /gene="gpmA"
                     /locus_tag="OO448_003115"
     CDS             10209..10961
                     /gene="gpmA"
                     /locus_tag="OO448_003115"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6338261.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11120..12172)
                     /gene="aroG"
                     /locus_tag="OO448_003116"
     CDS             complement(11120..12172)
                     /gene="aroG"
                     /locus_tag="OO448_003116"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6338262.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            12488..12868
                     /locus_tag="OO448_003117"
     CDS             12488..12868
                     /locus_tag="OO448_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6338263.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEKHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            12982..13923
                     /gene="zitB"
                     /locus_tag="OO448_003118"
     CDS             12982..13923
                     /gene="zitB"
                     /locus_tag="OO448_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6338264.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13920..14639)
                     /gene="pnuC"
                     /locus_tag="OO448_003119"
     CDS             complement(13920..14639)
                     /gene="pnuC"
                     /locus_tag="OO448_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6338265.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(14677..>14839)
                     /gene="nadA"
                     /locus_tag="OO448_003120"
     CDS             complement(14677..>14839)
                     /gene="nadA"
                     /locus_tag="OO448_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6338266.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3734 a         3927 c         3715 g         3463 t
ORIGIN      
        1 cagctaaaat tagtcgcttt tggcggtgaa tgctgaatta aaacgcagaa ttttgtccga
       61 tagtggtatc ggtgtaccat tcgcgcagac gaaaattctt aaatgacgcg gtaaaagcgc
      121 gcgccaacat tgtgattggt gataacacca ccgacagcat tgcccagttc atttatagcc
      181 acctgattta atcaggcggt tatcgacaca cttttaattt gcgcgtacag ccacaggcca
      241 ggtttgatcg ccagttcatc cctggcccac gggctgatac gcgcccacag cgttttaccg
      301 ccgacttcca gctccacttc cacctggccg ttgtcgtcat aactattaac aacttttgcc
      361 cgcaatacgt tacgaatgct ggtttgctgc ggcggttgta aaaccagaga aacatcggaa
      421 gcctgaatgc ggatacgtag cgcagcttgc agcggttcgt ccagcttatt gacccacaaa
      481 tgctgatcgc ccagcgccag cgcggtcatc gcgtaatgcg gatgatgctc aagtaccgtc
      541 actttcagaa tgctactttg ttgctctttc ggcagccacg gattcatcac gctactgccc
      601 cacacttcct ccagcgcgcc aaaggctttc acctgaccgt tttccagtac catcactctg
      661 tctgccagat ggaggatctc atccagcgaa tggctgacat acaacatcgg aatgttgatt
      721 tcccgtgtca gccgttgcag ataaggcaac agttcgcgtt tacgcggaat atccagtgac
      781 gccagcggtt catccagcaa cagcaattcc ggtgctgtca gcaaagcccg accaatcgcc
      841 acgcgctgtt tttcgcctcc ggacaggctg cctggtaaac ggtcaagcaa cggttcaatg
      901 cctaaaagcg ccaccagctt atcgaactga tcgaccatac ttttcgacat gccgtagcgc
      961 agattgccac gcactttgta atgcgggaac agccgcgcat cctgaaaaac atagccaacg
     1021 cgacgctttt ccggcgtcag gcagatacct ttttcggcat catttagtac ccgcccattg
     1081 aggacaatcc gccctttttg cgggcgcgtc agtccactga tggcgttaat cagcgaagtt
     1141 tttccggcac cggagacgcc aaagatagca gtgatgccat tggcgggcag tgtttcatta
     1201 atggtcaggc aatggttgcc caacgtctgg gaaaaattca gctccagcat gattagcgcc
     1261 ccgcccgttc acggctgatt ctggccagcc attctgaaat caacagggag atcatcgcca
     1321 gcgcaataga aataatgcac agtctcgccg cgccactttc cccgccgggg gtctggatca
     1381 gggtatacat ggcagaggga atggttcgcg tttcgccagg aatgttcgac acaaaggtga
     1441 tggttgcacc aaactcaccg agagaacgag caaaagccag taccgtacca acaataattc
     1501 ccggtaaggt cagcggtaac gtgatagtaa agaaaacgcg ccagcgcccg gcacctagtg
     1561 ttcttgcggc ctgttcgagt ttgacatcaa ccccttccag cgccagacga attgcccgca
     1621 ccatcagcgg aaacgacatg acggcggcag cgagaaccgc gccgcgccag ctaaaggcga
     1681 aggtaatacc aaaccagtca tacagacgtt caccgataaa tccgcgccgt cccatcgaaa
     1741 ctaataataa gtaaccgacg accacgggcg gtaacaccag cggtagatgc agtacgctgt
     1801 cgagcagagc tttgcccgga aacgtgcaac gcaccagtaa ccaggcaaaa aagatcccaa
     1861 acggcaggct aaacagcaca gccagggaag aaactttcag gcttaataaa actgcctgcc
     1921 attctggatc ggtcagtatc attacttggt tgtaaatccg taacgtttaa agatttccgc
     1981 tgcctgcggt cccttcagat aatcataaaa tgctttcact gtcgcattgt tatgcccttc
     2041 cacaacagca accggatatt ccactttttt atgtgaatct tccgggaagg tggcaaccac
     2101 ttttacccct ttgctgacaa ctgcgtcaga accgtaaaca atgcccaacg gcgcttcatt
     2161 gcgttcgacc aacgccagcg cgccacgaac atcttccgcc ggagccagtt tcggggagag
     2221 cgtatcccat gcgcccagtt tttgcagtgc ttcttttgca taaatgccag cagggacatg
     2281 ttccggatcg ccaaccgcga ggcgaccgcc attcagcagc gaagtccagt tagttgtgct
     2341 gtcgatgatg aaatctttct gctcgctggc tttcggtgct acaacgacca ggctattgcc
     2401 gagcagtgtc tggcgcgaag ccgtatcgat cgctttttta tcaaccgcat aatccatcca
     2461 tttctgatcg gcagagataa acagatccgc aggcgctccc gcttcaatct gacgggccag
     2521 agtagatgac gaagcgaaag aagaaaccac atccacgcct ttttcttttt tatactgcgt
     2581 agcaatgtcc tgcattgcgt tagtcagtga tgcggcggcg aacaccgtga ttttcccttc
     2641 atctgccagt gtattgccag caacagcgaa agagagtgcc gccccggcaa acaagttcaa
     2701 ccatttacga gccattggta accccttaat gtaacgttat ataggtgaca atgtaacggc
     2761 tagctcagaa ttttcccagc agttattgct aacctttgtg aggtagataa gaaaaaatat
     2821 cggctggaaa agcaggaagt tgagaaaaag aaatgcccga ctaagcgggc attcagggaa
     2881 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     2941 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     3001 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     3061 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     3121 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     3181 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg gatttcgcta ctaaaacaca
     3241 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     3301 cctgggatgc cattaacgag atgaatcagt taagcgagca tattctggtt gagcgcgcaa
     3361 caggcggtaa aggtggcggc ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     3421 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     3481 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     3541 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacattcaa cagcatgttg
     3601 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     3661 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     3721 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     3781 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     3841 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     3901 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     3961 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     4021 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     4081 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagcgggaaa
     4141 tcggccctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     4201 cagttttccc acatcactcg tctctccttc gagcaattgc aaaaactcgt cagcgacgaa
     4261 tggcagcgga ataacaccga tatgctcggc cctggcgaag atgacaccgg acgcactacg
     4321 gctgaaatca ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     4381 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     4441 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagtcg
     4501 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     4561 cagtccggta ttactctggt actggtgctc aatcgcttcg atgagatccc ggagtttgtc
     4621 cagtttgctg gcgtgctggc ggattgcacg ttagcggaaa ctggcgctaa agaggaactg
     4681 ctccaacaag cactcgtcgc gcaactggcg catagtgaac agcttgaagg tgtgcaactg
     4741 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaacc gcgcattgtg
     4801 ctgaacaatg gcgtggtttc ttataacgat cgccccattc ttaataacct tagctggcag
     4861 gtgaatccag gtgaacactg gcaaattgtc gggccaaatg gtgcaggaaa atcgacgtta
     4921 ttaagcctgg ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcgga
     4981 cgacgtcgcg gcagcggcga aaccatctgg gatatcaaaa agcatatcgg ttacgtcagc
     5041 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     5101 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     5161 cagtggctgg atattctcgg cattgataaa cgtacggctg acgctccgtt ccatagtctt
     5221 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     5281 cttattctcg atgaaccact acaggggctt gatccgctca atcgccagct tatccgccgt
     5341 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tatttgtttc gcaccacgct
     5401 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     5461 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     5521 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     5581 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     5641 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     5701 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     5761 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     5821 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     5881 agcgcttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     5941 tgaccgagat actgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     6001 ccgtcggcga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     6061 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     6121 ccaccgttta tggcgatcag cccaaaattc catacgttga aagcttcccg accggcacac
     6181 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     6241 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     6301 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     6361 cccaggttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     6421 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     6481 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     6541 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     6601 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     6661 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     6721 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     6781 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     6841 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     6901 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     6961 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     7021 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     7081 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     7141 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     7201 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     7261 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     7321 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     7381 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     7441 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     7501 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     7561 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     7621 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     7681 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     7741 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     7801 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     7861 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     7921 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     7981 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     8041 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     8101 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     8161 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     8221 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     8281 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     8341 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     8401 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     8461 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     8521 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
     8581 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
     8641 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga tctgaaacgt atgggcgagt
     8701 tgatggcgga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
     8761 acactctggt agaaatcgtc aaagcagtga ttggcgacaa aggtggcgta cgcatgaccg
     8821 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
     8881 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
     8941 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
     9001 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
     9061 ctggggtgcg actttacttt cagctcgtat tccgctttcc gatggcagcg tccgcgaggc
     9121 gctgctcggc tgtgccagcc cggaatgcta tcaggatcag gccgcgtttc tgggggcctc
     9181 tattggtcgt tatgccaacc gcatcgccaa tagccgttat acctttgacg gtgaaacggt
     9241 gacgctttcg ccaagtcagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
     9301 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
     9361 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
     9421 cgataaccgt atctccatta cttatcgcgc cacagttgat aaatcttgcc cggtgaatat
     9481 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
     9541 gcagattctg gcgggcgaat atctgccggt tgatgaaggc ggcattccgc acgacggcct
     9601 gaaatctgtc gccggaacct cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
     9661 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
     9721 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
     9781 gaaggtctac accaccgctc ccgctcttca attctactcc ggcaacttcc tcggcggcac
     9841 accgtcgcgg ggaaccgaac cttacgccga ctggcaagga ctggcgttgg aaagcgagtt
     9901 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
     9961 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    10021 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    10081 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    10141 gctgttgctt cgtcacggca atataatgag aattattatc ataaaaagat aatttgagga
    10201 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    10261 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    10321 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    10381 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    10441 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    10501 tgaacaaagc ggaaaccgcc gaaaagtatg gcgacgagca ggtgaaacaa tggcgtcgtg
    10561 gttttgccgt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    10621 cgcgttacgc gaaactgagc gagaaagagc tgccgctgac tgaaagcctg gcgctgacca
    10681 ttgaccgcgt gatcccttac tggaatgaaa ctattctacc gcgtatgaag agcggtgagc
    10741 gcgtgatcat cgctgcacac ggtaactctt tacgtgcgct ggtgaaatat cttgataaca
    10801 tgagtgaaga agagattctt gagctgaata tcccgaccgg cgtgccgctg gtgtatgagt
    10861 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcag
    10921 cgaaagcagc ggcggttgct aaccagggta aagcgaagta aacgtcattc gtttaaaaat
    10981 gagaaagccg actgcaagtg agtcggcttt tttgttgcta acaatggagc acattgcctg
    11041 atgcgacgct gcgcgtctta tcaggcctgt ggtgattcat cggatacgcc actctgacgg
    11101 cgcatccgac aattaaacct tacccgcgac gcgcttttac tgcattcgcc agttgacgta
    11161 acagagcatc ggtatcttcc cagccaatgc aggcatcggt gatgctctta ccgtaggcca
    11221 gcggctcccc gctatcgagg ctctgattgc cttccaccag atggctttcc accatcacgc
    11281 caataatggc cttttcgcca ccggcaatct gctggcaaac gtcagcacaa acatccatct
    11341 gctttttgaa ttgtttggac gagttagcat ggctgaaatc gatcatcacc tgtgctggca
    11401 ggcctgcttt gttcagccct tctttcactt cagcaacgtg cttcgcgctg tagttaggct
    11461 ctttaccgcc gcgcagaatg atatggcaat caccgttacc gctggtattc acaatcgccg
    11521 aatgccccca tttagttacg gacaggaagc agtgcggcgc accggcggca ttaatggcat
    11581 cgatagccac tttaatcgta ccgtctgtgc catttttaaa acctaccgga caagaaagac
    11641 cagacgccag ttcgcggtgc acctgcgatt ccgtggtacg tgcaccaatt gcgccccagc
    11701 tcatcaggtc agcgagatac tgaggagtga tcatatcgag aaactcaccc gccgctggca
    11761 gaccgctgtc gttaatatca agcagcaatt tacgggctat acgcagaccg tcgttgatct
    11821 ggaagctgtt atccatatgc ggatcgttaa tcaacccttt ccagcccacc gtggtacgcg
    11881 gcttttcaaa atagacgcgc attacgattt ccagctcatc tttcagctct tcacgcagcg
    11941 ccagcaagcg agtggcatac tctttagccg cgacaggatc atgaattgag cacgggccaa
    12001 tcaccaccaa caggcgatca tcattacctt tcaggatctt atggatcgct tttcgggcat
    12061 gggcaaccgt attcgcggca ttttcagtta cggggaattt ttccagcaat gcgacaggag
    12121 gaagtaactc tttgatttct ttgatgcgta aatcgtcgtt ctgataattc atgtctgttc
    12181 cagtgttgcc atacttatct tagtgaatgc aatacttcca atctatatct tccgtcagaa
    12241 tgtgtaaacg gggttttaca ctatgaacgg attaatcctg gaattagcaa gaaaaacgcc
    12301 agattgtcgc gaaaaacgag atctctccta caatttctaa ctgtaactcc tttgtttgtt
    12361 aattatttca agattctctg ctgcgtttca taaactggct gaaatcttaa accaatgcct
    12421 tatattcacc tgcaaatgca ctgttggaag aggttatccg acataacgac cataacagga
    12481 gcatcctatg aaaatgacaa aactggccac attatttctg actgccactc taagccttgc
    12541 cagcggtgcc gcactggctg ccgatagcgg agcgcaaact aataacggcc aggcaaacgc
    12601 cgcagctgat gcgggccagg tagcccctga cgcccgtgaa aatgtcgcgc caaataacgt
    12661 cgacaataac ggggtaaata ccggttctgg cggcacaatg ctgcattcgg atggttcttc
    12721 aatgaacaat gacggaatga ccaaagatga aaagcacaag aacaccatgt gcaaagatgg
    12781 tcgatgtccg gatatcaata aaaaggtaca aatcggtgat ggcatcaata atgatgtcga
    12841 taccaaaacc gacggtacca cgcagtaacc ctgcaaaata gccaataatg ggagagcgga
    12901 gtctctccct tttaattttt cacttctaaa agcagtatga tgtcataaag ttaagtgaga
    12961 ataattaagg gatgactcgt catggcgcac tcacactcac acacgtcctc acacctgcca
    13021 gaagataata atgctcgtcg cttgttgtat gctttcggcg tgactgccgg gtttatgctg
    13081 gtagaagtcg ttggtggttt tctttctggt tctctggcat tgctggccga tgcgggtcat
    13141 atgttgaccg atactgccgc cctgcttttc gccctactcg ccgtgcaatt ttcacgtcgt
    13201 cctcccacca ttcgccacac tttcggttgg ctaagactga ccacgctcgc ggcgtttgtg
    13261 aatgccatcg ccctcgtggt aattaccatt ttgattgtct gggaggcgat agaacgcttc
    13321 cgcacgccgc gtccggtcga gggcggcatg atgatggcaa ttgccgtggc tgggttgctg
    13381 gcaaatatac tttctttctg gttacttcat cacggcagcg aagagaaaaa cctcaacgta
    13441 cgagcagcgg cattgcatgt gctcggggac ctgttgggtt cggttggggc tattattgcc
    13501 gcgttgatta ttatctggac cggctggaca cctgctgacc ccattctctc gatagtggtg
    13561 tcgcttctgg ttctgcgtag cgcgtggcga ctgttgaaag atagcgtgaa tgaattactt
    13621 gaaggtgcac cggtatcgct ggatatcgct gaactgaagc gtcgtatgtg ccgggaaatc
    13681 ccggaagtcc gcaatgtgca ccatgtacat gtatggatgg taggcgagaa gccggtgata
    13741 acgctgcatg tgcaggtgat cccgccacac gatcacgatg ccttgttgga tcagatccaa
    13801 cactacctga tggatcacta tcagattgag cacgccacca ttcagatgga atatcaacct
    13861 tgtcatgggc cagactgcca cctgaatgaa ggggtgtctg gccattcaca tcatcaccat
    13921 taatgggaca gcgcgcgtga gcctctttca cgtgcgctgt tgatccacat ccgactgccg
    13981 ttgagcgcaa taaaggtcag gatgatgtac tccagagaca tggcgtaaac gccctgaagt
    14041 gcaaaaataa cgacgctaat cacgttaata atcacccaca acagccagtt ttccacatac
    14101 ttacgcgtca tcagaatcat tgccacgata gataacacca tcatgcatga atcccagaac
    14161 gggaaagcgt ccggttgcag ttcaggcatc acaacctgta atcctaatgc ttgcatgatc
    14221 atgactgcca cgcgggtcaa aaatgcaaac accggattga taaataccgt catcagacca
    14281 atcgaaacaa cgcaaaccgc caaccagctg agcgccttcg gcaatggtaa ccagcgaatt
    14341 ttcaactccg cctcgttctg actggtttgt cgcgaccacg cataccaacc gtaaatattc
    14401 gcggcaaaga aaaacacctg taatagcagg ctggcataca gctgaatctg aaagaaaata
    14461 atgccaaaca aggtgacgtt gatcaggcca aagaagtagt tgctgatctt ctccagactg
    14521 gcaaggccaa tacacagcaa cccggcgatc gtgcctaccg cttcgatcca tgagagatca
    14581 taaccgcctg cccctattgg tatatgtacc aggatattct gcacactaaa aaaatccatc
    14641 tttcccccaa agcgtaacgt tacgccttat tattcgttat ccacgtagtg tagccgcaaa
    14701 atccagcata cgattgagcg gcaccagcgc cctctctcgc agcctttcat caagaacctc
    14761 gtgattgctt ccttcctgtt ctaatgcctc tgcgatggac tgaaggccat tcatggccat
    14821 ccacggacaa tgcgcgcag
//
LOCUS       DAJTVJ010000104        14508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000104 DAJTVJ010000000
VERSION     DAJTVJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14508
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.104"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="OO448_003121"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="OO448_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="OO448_003122"
     CDS             1909..3261
                     /locus_tag="OO448_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCR6338267.1"
                     /translation="MNSNVLTQTIVTGSDPRGLLEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="OO448_003123"
     CDS             3285..3767
                     /locus_tag="OO448_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6338268.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3811..4766
                     /locus_tag="OO448_003124"
                     /pseudo
     CDS             3811..4766
                     /locus_tag="OO448_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4776..5255
                     /locus_tag="OO448_003125"
     CDS             4776..5255
                     /locus_tag="OO448_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338269.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(5392..6177)
                     /locus_tag="OO448_003126"
     CDS             complement(5392..6177)
                     /locus_tag="OO448_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338270.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(6507..6583)
                     /locus_tag="OO448_003127"
     tRNA            complement(6507..6583)
                     /locus_tag="OO448_003127"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6547..6549),aa:Asp,seq:gtc)
     gene            complement(6716..7447)
                     /gene="dnaQ"
                     /locus_tag="OO448_003128"
     CDS             complement(6716..7447)
                     /gene="dnaQ"
                     /locus_tag="OO448_003128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6338271.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            7512..7979
                     /gene="rnhA"
                     /locus_tag="OO448_003129"
     CDS             7512..7979
                     /gene="rnhA"
                     /locus_tag="OO448_003129"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6338272.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(7976..8698)
                     /locus_tag="OO448_003130"
     CDS             complement(7976..8698)
                     /locus_tag="OO448_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6338273.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            8732..9487
                     /gene="gloB"
                     /locus_tag="OO448_003131"
     CDS             8732..9487
                     /gene="gloB"
                     /locus_tag="OO448_003131"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6338274.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            9559..10917
                     /gene="mltD"
                     /locus_tag="OO448_003132"
     CDS             9559..10917
                     /gene="mltD"
                     /locus_tag="OO448_003132"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6338275.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(10966..11562)
                     /locus_tag="OO448_003133"
                     /pseudo
     CDS             complement(10966..11562)
                     /locus_tag="OO448_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(11593..12393)
                     /locus_tag="OO448_003134"
     CDS             complement(11593..12393)
                     /locus_tag="OO448_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6338276.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            12634..13548
                     /locus_tag="OO448_003135"
     CDS             12634..13548
                     /locus_tag="OO448_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6338277.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(13545..14348)
                     /gene="dkgB"
                     /locus_tag="OO448_003136"
     CDS             complement(13545..14348)
                     /gene="dkgB"
                     /locus_tag="OO448_003136"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6338278.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         4021 a         3409 c         3443 g         3635 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgagtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgctgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgaca cttgatgttg caaaaaaaaa aaaaaaaaaa
     4021 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa tactcagccc aataacatca tttgattaca
     4081 ttcattttat cactacacat ccttcaggta taaaagatac actcgcctgg ttagttaacg
     4141 cgagtaaatt aatgactgag tttgatgaca atgggaaaat tatatttaac attaatgcct
     4201 taaagtatac aaaagcaagt tactttgaaa tacttggaga gaaatatata aaaatcacaa
     4261 catccagccc ctggctttta gaaaagctcg gtaaatatat tttctcttca cgagcgcctc
     4321 aagttctgga actcgctatt ggctggcgag gagctttatc tgaatcaata aaaggagtta
     4381 agttctgtat ctggttttca gtagcctgga gaaccataga atttattatg agttcagagc
     4441 gagatctggt taattttctt ggcgattttt caatggatgt ggccaaagca gttatagccg
     4501 gtggtgttgc aaccgctatt ggaagcctgg cttcttttgc ctgtgttagc tttggctttc
     4561 cagtaattct tgtcggagga gcaattttac tgacagggat agtgtgtaca gttgttttaa
     4621 atgaaatcga tgctcaatgc catttatcag aaaaattaaa atatgcaatt agagatggac
     4681 taaaacggca acaggaactt gataaatgga aaagggaaaa catgactcca tttatgtatg
     4741 ttcttaacac tccacccgtg atatgaggat taaccatgcg tttacatgtt aaattaaaag
     4801 agtttctttc gatgtttttt atggccatat tatttttccc tgcattcaat gcttctttgt
     4861 tttttaccgg agttaaacct ctatattcaa taataaaatg ctccacggaa atattttatg
     4921 attggagaat gttgattctg tgctttggtt tcatgtcgtt ttccttttta aatattcatg
     4981 taattttact tacgataata aaattattct taatcaaaaa aacaaaggta gtcaatttcg
     5041 ccacagacat aacaatacag ctaactctta tatttttact aatagctatc gtaatagcac
     5101 ctttaatagc tccttttgtc acaggatatg tcaatgctaa ttatcacccc tgcggtaata
     5161 atacgggaat tttcccagga gcaatctata ttaaaaatgg catgaaatgt aataatgaat
     5221 atatttctcg caaggaagat tcagcagtta aataattaaa cattctaaaa caaacagttt
     5281 ttaactccgg attccatgtc ctttcttgca ctgtctttgt caagaaaaat ttcttatgca
     5341 aagaattttg tattatttcc gccagaaatt cctggcggca gatataattt attattgata
     5401 gcggttaacc aaatctttta ccatcgcttt ataggtcttt tgcttaagtt gcatgatttg
     5461 ctgctctgtc atatcagtcg tactggcttc attcttgtcg ggcaatggtg gaagcacttt
     5521 atattcataa ttcaccggcg accaggcagc ccactcacct gtagctacat ccaccagcgt
     5581 gaatcgtacc agatagcgca gtgacatcgt gtcagtcaat ttttgatttt tataatctga
     5641 ccaaactatt gatttcaatc cagagtcata ttttcctgtc tgcaacgtat cctggaaaac
     5701 aataatcgct ttctgatgcc cttttgcagc cacaaaacgc agtgcctgca tccagttcat
     5761 attctcagca ctggcaacat cctcgttctc atttttatct ttgtttttgt tacaagtcag
     5821 aggcttttgc ctgtccggga taccagagaa tgtggaaaca gtataatatt ttcgcatctc
     5881 ctcctgcata atggtttccg gtgcgcggtt gccggattga acaagtatta caggagaatt
     5941 aaggggaacg cggaactcat ctccatcaag cgcagcttga atatcctctt cggatacggc
     6001 cagagtggtt tcattaccga ataaatcttt gtctgtcagt tgctggggag tgtttttatt
     6061 aatattactt tgtgtgactt gagctttttt cttcttttca tcaatagaag attcagagac
     6121 acatcctgca agcagcgaga ataatacaca tatacaacaa acccttctta aattcataat
     6181 cggacactcc ctcgcctgtc ataatcatgg taatgtttag ctaacgattt ttaattatat
     6241 actccacttg aatcagaaca aacaaaaata acagtatata taaattcatt aaatcaatag
     6301 aaaaatgaaa atcataacat tcaaatatat ttaaattgaa tgatttgata gtatgattaa
     6361 atattaatga tatggtctac catatgtgat taacacattt acagcataac aaaaccatag
     6421 accgaagaaa tagcgggatt taaatgcaaa aaaaccgcaa aattaaaaat cttgcggttc
     6481 tctgaactca ttttcatgag tgaatttggc ggaacggacg ggactcgaac ccgcgccccc
     6541 ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccgcgttgt gttccgttgg
     6601 gaacgaggcg aatattacgg attgcctcac ccttcgtcaa cggtttttct catcttttga
     6661 atcgtttgct gcaaaaatcg cccaagccgc tatttttagc gccttttaca ggtatttatg
     6721 cccgccagag gcagcttccg cccttcttct ccaccagatc aagacgggct tcatgagctg
     6781 caagctcttc atctgtcgca aaaacaacgc gtaacttact tgcctgacgt acaatgcgct
     6841 gaattgttgc ttcaccttgt tgctgctgtg tctctccttc catcgcaaaa gccatcgacg
     6901 tttgaccacc ggtcatcgcc agataaactt ccgcaaggat ctgtgcatcg agtaatgccc
     6961 cgtgtagcgt tcgtttactg ttatctattt cgtagcgggc acataacgca tcgaggctgt
     7021 tgcgcttacc aggaaacatt ttcctcgcca ccgcaaggct atcggtgacc ttacagaagg
     7081 tattggtctt cggaatatcg cgcttaagca acgaaaactc gtagtccata aagccgatat
     7141 cgaacgctgc gttatggatc accaactccg cgccgcggat atagtccatg aactcatcgg
     7201 ctacttcggc aaacgtgggc ttatcgagca aaaattcatc ggcaatacca tgtacgccaa
     7261 aggcttccgg atccaccagc cgatcgggtt tgagataaac atggaagtta ttgcccgtca
     7321 ggcgacggtt caccacttca acggcaccaa tctcaatgat cttgtggcct tcatagtgcg
     7381 caccaatctg gttcataccg gtggtttcgg tatcgagaac gatctggcgt gtaattgcag
     7441 tgctcatagc ggtcatttat gtcagactta tcgttttaca gttcgattca attacaggaa
     7501 gtctaccaga gatgcttaaa caggtagaaa ttttcaccga tggttcgtgt ctgggcaatc
     7561 caggacctgg gggttacggc gctattttac gctatcgcgg acgcgagaaa acctttagcg
     7621 caggctacac ccgcaccacc aacaaccgta tggagttgat ggccgctatt gtcgcgctgg
     7681 aggcgttaaa agaacattgc gaagtcattt tgagtaccga cagccagtat gtccgccagg
     7741 gtatcaccca gtggatccat aactggaaaa aacgtggctg gaaaaccgca gacaaaaaac
     7801 cagtaaaaaa tgtcgatctc tggcaacgtc ttgatgctgc cctagggcaa catcaaatca
     7861 aatgggaatg ggttaaaggc catgccggac acccggaaaa cgaacgctgt gatgaactgg
     7921 ctcgtgctgc ggcaatgaat cccacactgg aagatacggg ctaccaagtc gaagtttaag
     7981 cttgtggttt acgacattgc cgggtggctc caaccgcctg gcgaattcgt ggcttgtttt
     8041 tactctgttt catcgggttt agcgttaaag gaatagtccg tttccgggca acaataagtt
     8101 gtaagcaacc aagcgcagga atatgcgcat tcaatagttt tcctccgtgt ttgttccacg
     8161 ggagaacgtg gaaacggctg gcgtgcagca cttcaaaatt caacaaagag agccagtcca
     8221 gttgccgcat cagagtaaac atccggctgt tatagggcga ggtttttcgc aataccggca
     8281 caagtttgcg taatcccatt aaactaatgg gattgaagcc actaatcacc agccagccat
     8341 catcaatcaa tacccgatcg gtttcacgca ataaacgatg cggatcggtg caccacggca
     8401 atgtatgtgc cagtagacaa acatcaacgg atttatcggc aaaaggaaga tgaagtggat
     8461 ccgcctgcac ctggacgggc attccttgcg cagaaacatt cacttgatga gaaaccgcgc
     8521 acgcttcgca attgatttct gcgcttaaat tgccaatctt aagcagatga aaaccataca
     8581 ttttagtgaa ccacgggttg agctggcgct ccagcgcctt gcgataaagc tctccccagg
     8641 gcaaatcgcc ccagcgatca ggagccacga cagtttgagg gactcttgcc ggtttcatca
     8701 caaccttccg tttcacacta agaggtaatc tatgaatctt aacagtattc ccgcctttga
     8761 tgacaattac atctgggttt tgaatgatga agcaggtcgc tgcctgattg tcgatcccgg
     8821 agacgcagag ccagtattaa acgccattgc cgccaataac tggcaaccgg aggccatatt
     8881 tctcacccac catcatcacg atcacgttgg cggcgtaaaa gaactggtgg aaaagtttcc
     8941 gcaaattgtg gtgtatggtc cacaagagac acaagataag ggaacaacac aggtagtcaa
     9001 agatggcgaa actgccttcg ttttggggca tgaatttagt gtaattgcca caccgggtca
     9061 cactttagga catatctgtt acttcagtaa accttatcta ttttgcggcg acacgctgtt
     9121 ttctggtggg tgtggtaggt tgtttgaagg gacagcatca caaatgtatc aatcacttaa
     9181 taagttaagt gcgttacctg acgatacatt ggtatgttgt gctcatgaat atacgttatc
     9241 aaatatgaag tttgctttga gtattcttcc gcacgatttg tccataaatg attattatcg
     9301 taaagttaag gagttacggg caaaaaatca aataacacta cccgtaattc tgaaaaatga
     9361 gcggcaaatt aatgtttttt taagaacgga agatattgat ttaattaatg taattaatga
     9421 agaaacatta ttgcaacaac ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag
     9481 gttctgataa aactttcttg tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa
     9541 gcaactattg acacacacat gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg
     9601 ggttgccaga gtaccggcaa cgttcaacag cacgcacaga gcctttctgc agctggtcaa
     9661 ggggaagcag caaagtttac aagtcaggca cgatggatgg acgatgggac gtctatcgcg
     9721 ccagatggtg acttgtgggc tttcattggc gacgagctaa agatgggaat tccggaaaat
     9781 gaccggattc gcgaacagaa acagaaatat ttacgcaata agagctatct ccacgatgta
     9841 actttacggg cagagccgta tatgtactgg atagcagggc aagttaaaaa acgtaacatg
     9901 cctatggaac tggtactact acccatagtg gagagcgctt ttgatcctca cgcaacgtct
     9961 ggcgccaatg ccgcaggcat ctggcagatc attccgagca cggggcgcaa ttatggtttg
    10021 aaacagaccc gcaattatga cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg
    10081 aatatgatgc agcgtctgaa caaaatgttt gacggcgact ggcttctgac cgtagcggct
    10141 tataacagcg gcgaaggtcg ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa
    10201 tccacggact tctggtcgtt accgttgccg caggaaacga agcagtacgt gcctaaaatg
    10261 ctggcattga gtgatattct caaaaacagc aagcgttatg gcgtacgtct gccaacgacc
    10321 gatgaaagcc gtgctctggc gcgtgtgcac ctgagtagcc cggttgaaat ggcgaaggtt
    10381 gcagatatgg cggggatttc cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc
    10441 tccacgctgg gcgcaagtgg gccgcagtac gtgatggtgc caaagaagca tgcagatcaa
    10501 ctgcgtgaat ctctcgcttc aggcgaaatt gctgctgtac agtcgacgct ggttgccgac
    10561 aatacgccgc ttaacagccg tgtttacacc gtacgctctg gcgacacgct ttcaagcatc
    10621 gcttcacgtc tcggcgtaag caccaaagat ttgcagcagt ggaacaaact gcgcggatct
    10681 aagctgaagc caggccaaag tttgacgatt ggtgcaggca gtagcgcaca gcggttggca
    10741 aacaacagcg atagcattac gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct
    10801 aaacgccacg gcgtgaacat caaagatgtg atgcgctgga acagcgacac tgcgaatctg
    10861 caaccaggcg ataagctgac gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa
    10921 accagataat aaaaaaggca ccgattcccc cggtgccttt tttatttatg ccgctttatg
    10981 tgcttctacc atgatgatat cactggtgaa agagccatca gtctgcaagg aaaaatacgt
    11041 tctcacctct gtcgatgcgc tctgttggta aatgcgaata gcgtctacta acgcttctgg
    11101 cgtacgcatt ctcacgaccc atgaagaaaa ttccagcggt aacttatctg taattaaatt
    11161 atcaactatc agattggctt cattgattaa cgtcaaccac gcaccgctgg cgtagtttcg
    11221 tacgtgagag gtatcgcgta atgcttctac cgcctgtaac cagatgtcgc gcactgggtg
    11281 acccggagac attacatcca tcacaatcag cctaccgcca ggtttcaata tcctattcac
    11341 ttctcgcagt gctgcaccaa catcatgcca gtgatgggca gaataacggc tgataacaat
    11401 atcaaatgcg ttatcggcaa atggcagact tgcggcatat ccctggcggg tggtgatatt
    11461 tttcagttgc cgggcttcgg cggcttgtgc cacgacatcc agcatgtggg cagataagtc
    11521 atacgccacc accgcgctca cgttttgcgc agcgccaaac gttggtctgc cctgcggcag
    11581 acctgacata ccttatttat caggcttgcc gggactgaat tcaacgagta gcggattgtg
    11641 atcggaagcg cgcgtaacca gtacagaagc ttcactgacg ttcagaccac ggtagaaaac
    11701 aaaatcgagc gggcgaccaa acgcccgacg gcgctgatca tcggtaaaac gcacctggcg
    11761 cagcgacatt tcccgcgcaa agcgatataa cgcgttcatc cttctacggc tccaggcatt
    11821 gaaatctccc gccataatga ccgggccgct gtggtgagct atctgatcac caataggaag
    11881 taactgctta ctatagacat ccacgcccag actgaagttg acggcgtgta tattaaccac
    11941 catcaacagg cgggtgtcag gtaatggata gaccgtcacc agtgccgact tcgccagacg
    12001 caaaatgggt tctcgttcac gtaacgggca gcaatacact ggatgtgccg ccgaaagggt
    12061 cattacgcca gaaggatgtt gtggcagcac gaaagcgggt acctgatcgg cggcaagata
    12121 gttagcggtc gcaaactgta ccaactctgg cgttgtttgc gcttcctgca ataacaccag
    12181 atgtgcgtct ttgccgtaat tctttaatac cgacaaccat tcagcgcgtt gctgtttgta
    12241 tatgttccac accaggatcc gaatacgctc ttcggtactt aacggttctc cgggtggtaa
    12301 tgcctggccg atgctcgcaa aagaccccgg cggtaagatc ctctccgcag gttgtccggc
    12361 aacatagcgc atggcatagg tgttttttcg cacttttgac tttgaaacct ctattacgtc
    12421 aacccttccg cactcgggag ggttctactg ttatagggat tttagctcac tctttcaacg
    12481 agcaattgta tattgttatg taagcaagtg cttacaccgg tcagaaattt gctcattatt
    12541 gagaacatta aatcggatga catatcacgc atcctgatgg tttaatgaca aataaattca
    12601 aaatatctca tctattttgc aacaggagca aaaatgaaag ccacgtcgga agaactcgcc
    12661 atttttgttt cggtcgtcga aagcggcagc tttagccggg cagcggaaca attagggcaa
    12721 gcaaactcag cggtaagccg ggcggtgaaa aagctggaaa tgaaacttgg cgttagcctg
    12781 cttaatcgga ccacgcggca acttagcctg acggaagaag gcgagcgtta ttttcgtcgc
    12841 gtacagtcaa ttttgcagga gatggcagcg gcagaatcag aaattatgga gacgcgtaat
    12901 acaccgcgtg gactgttacg gatcgatgcc gcaactccag tggtgctgca ctttctgatg
    12961 ccgttaatta agcctttccg tgaacgctat ccggaagtca ctttatcgct agtctcctcc
    13021 gaaacgatta ttaatttgat cgaaagaaaa gtggatgtcg cgatacgcgc tggtacgtta
    13081 acggactcca gcttacgtgc caggccgtta tttaacagtt atcgaaaaat tatcgcctcc
    13141 cccgattata tttcccgcta cgggaagcca gaaacgatcg acgatttaaa gcaacatgtt
    13201 tgcctgggat tcactgaacc cgcttccctc aatacctggc cgatagcctg tagtgatgga
    13261 caattacatg aggtgaagta cggtttgtca tccaatagtg gggaaacact gaaacagctt
    13321 tgcctgagtg gcaacgggat tgcgtgtttg tccgactaca tgatcgacaa agaaatcgct
    13381 cgcggagaat tggtggagtt aatggcagat aaagtgttgc cagtggaaat gccattcagt
    13441 gccgtctatt acagcgaccg tgcggtaagt acgcgcatcc gggcttttat cgatttcctt
    13501 agcgagcatg taaaaacagc tcccggagga gctgtcagag aggcttaatc ccattcagga
    13561 gccagacctt ccgggctaac caggcggtcg ttgcaatcca gtgcggcgat cgctttttta
    13621 tcttcggcat caagctgtaa attttgtgcc ttaagattac tttccaggtt tttacgttta
    13681 gtagaagaag gaattactga gtaaccttcc cccatagccc acgccagaat cacttgtgcc
    13741 ggagtcgcat tgtgtttagc tgcaatacga gcaataacct catctttcag tgccttacca
    13801 taagccagcg tcatatagga agtgatatga ataccgtgct gtttagccca ggcaaccact
    13861 ttacggtttt gcagataagg agagagttca atctggttgg tagcgatgtt ttcagcgcca
    13921 acagcagcaa tcgctttttc catcaacggg atcgtgaagt tggaaatacc gatctcacgt
    13981 gtcagacctt gttttttggc ctccagcagc gcctgcataa actcttcaac agagacttca
    14041 tcgtttggtg acggccagtg gattagcgtc agatcaacat aatcggtacg caatttttgc
    14101 aggctctctt ttagactcgg gatcaatttg tctttgctga gattttcaat ccagatttta
    14161 gtggtgatgt agagttcatg acgtggcacg ccactttctg caatcgcctg acctactgcg
    14221 gcttcgttat catagatttg tgcagtatca attgcgcgat aaccaagttc aagcgccgtt
    14281 ttcacagatg aaataacaac gtcgtctttg agacggaaag tacctaaacc aaatgcaggg
    14341 atagccataa tatgcctctt ttaatgctat tttcgttaac tgaggtgatt atgggcttca
    14401 gactgttgcg gaaaaagagc ataaaatgca gaggattttt gcgattctgg caataataga
    14461 tatacagacg aaaaaaaacc tcgagcgtta actcgaggct tcttaata
//
LOCUS       DAJTVJ010000105        14469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000105 DAJTVJ010000000
VERSION     DAJTVJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.105"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="OO448_003137"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="OO448_003137"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6338279.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OO448_003138"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="OO448_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6338280.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OO448_003139"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="OO448_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6338281.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OO448_003140"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="OO448_003140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6338282.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OO448_003141"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="OO448_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6338283.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="OO448_003142"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="OO448_003142"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6338284.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="OO448_003143"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="OO448_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6338285.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="OO448_003144"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="OO448_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6338286.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="OO448_003145"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="OO448_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6338287.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="OO448_003146"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="OO448_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6338288.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSSVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="OO448_003147"
     CDS             complement(11403..11900)
                     /locus_tag="OO448_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6338289.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="OO448_003148"
     CDS             complement(11897..12259)
                     /locus_tag="OO448_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6338290.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="OO448_003149"
     CDS             complement(12249..12596)
                     /locus_tag="OO448_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6338291.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /locus_tag="OO448_003150"
     CDS             12705..13154
                     /locus_tag="OO448_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338292.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13403..>14469)
                     /locus_tag="OO448_003151"
     CDS             complement(13403..>14469)
                     /locus_tag="OO448_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6338293.1"
                     /translation="YIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKN
                     FFQNRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNV
                     TVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQ
                     KKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVI
                     EDLKVSNMSKSAAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLA
                     VPPAYTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGE
                     MVQSGRSLKQEPTEMIQATA"
BASE COUNT         3415 a         3993 c         3744 g         3317 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggt ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ccccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattgc tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc agcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cagcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctagggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtggc cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc aggctgaccg tacccgctgc tgactttgac atgttgctga
    13801 ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt
    13861 gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct
    13921 gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca
    13981 gcttcttctg gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg
    14041 tggcgagttt agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag
    14101 ttgatacttc actttctgtc tgaatactga tgtaccactt accgcaggac tggctgacag
    14161 tgacattttt cacaacaccc gtgacctgac ggctgttccg gtagcgcatc cagcccagtt
    14221 tcggcagaaa aatacggctg ttttcctgat cgagcttaac gccctgcggg tagcggaata
    14281 catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgattctgg aagaagtttt
    14341 tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtgacggg gcatctttaa
    14401 gccattgcgt ttcagtggca tttttccact caaccagcca ggaagccatt ttcccgtaag
    14461 ggatgtatt
//
LOCUS       DAJTVJ010000106        14404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000106 DAJTVJ010000000
VERSION     DAJTVJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.106"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO448_003152"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO448_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6338294.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO448_003153"
     CDS             1828..3147
                     /locus_tag="OO448_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6338295.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="OO448_003154"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="OO448_003154"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6338296.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="OO448_003155"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="OO448_003155"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR6338297.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="OO448_003156"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="OO448_003156"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR6338298.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="OO448_003157"
     CDS             8179..9729
                     /locus_tag="OO448_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6338299.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="OO448_003158"
     CDS             9731..10753
                     /locus_tag="OO448_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6338300.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /locus_tag="OO448_003159"
     CDS             10750..11646
                     /locus_tag="OO448_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6338301.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="OO448_003160"
     CDS             11643..12629
                     /locus_tag="OO448_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6338302.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="OO448_003161"
     CDS             12658..13548
                     /locus_tag="OO448_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6338303.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO448_003162"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO448_003162"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6338304.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3577 a         3557 c         3630 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcacacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttatcaggca
    14401 gaaa
//
LOCUS       DAJTVJ010000107        14306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000107 DAJTVJ010000000
VERSION     DAJTVJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.107"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="OO448_003163"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="OO448_003163"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6338305.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="OO448_003164"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="OO448_003164"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6338306.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OO448_003165"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OO448_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR6338307.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="OO448_003166"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="OO448_003166"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6338308.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="OO448_003167"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="OO448_003167"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6338309.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OO448_003168"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OO448_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6338310.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OO448_003169"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OO448_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6338311.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OO448_003170"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OO448_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6338312.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="OO448_003171"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="OO448_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6338313.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="OO448_003172"
     CDS             complement(13987..>14306)
                     /locus_tag="OO448_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6338314.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAJTVJ010000108        14158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000108 DAJTVJ010000000
VERSION     DAJTVJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.108"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..228)
                     /locus_tag="OO448_003173"
     CDS             complement(<1..228)
                     /locus_tag="OO448_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR6338315.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="OO448_003174"
     CDS             385..561
                     /locus_tag="OO448_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6338316.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="OO448_003175"
     CDS             1187..1486
                     /locus_tag="OO448_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338317.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OO448_003176"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OO448_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6338318.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="OO448_003177"
     CDS             complement(2472..2939)
                     /locus_tag="OO448_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6338319.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="OO448_003178"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="OO448_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6338320.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="OO448_003179"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="OO448_003179"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6338321.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="OO448_003180"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="OO448_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6338322.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OO448_003181"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OO448_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6338323.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="OO448_003182"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="OO448_003182"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6338324.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="OO448_003183"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="OO448_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6338325.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="OO448_003184"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="OO448_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6338326.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OO448_003185"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="OO448_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6338327.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="OO448_003186"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="OO448_003186"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6338328.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13837..14082)
                     /locus_tag="OO448_003187"
     CDS             complement(13837..14082)
                     /locus_tag="OO448_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6338329.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3446 a         3424 c         3689 g         3599 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg acgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cttggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgt
//
LOCUS       DAJTVJ010000109        14110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000109 DAJTVJ010000000
VERSION     DAJTVJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.109"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..1374)
                     /gene="hprS"
                     /locus_tag="OO448_003188"
     CDS             complement(16..1374)
                     /gene="hprS"
                     /locus_tag="OO448_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6338330.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1374..2045)
                     /gene="hprR"
                     /locus_tag="OO448_003189"
     CDS             complement(1374..2045)
                     /gene="hprR"
                     /locus_tag="OO448_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6338331.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFSEKLIATIRGMGYSF
                     VAVKK"
     gene            2178..2591
                     /gene="hiuH"
                     /locus_tag="OO448_003190"
     CDS             2178..2591
                     /gene="hiuH"
                     /locus_tag="OO448_003190"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6338332.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2700..3704
                     /gene="msrP"
                     /locus_tag="OO448_003191"
     CDS             2700..3704
                     /gene="msrP"
                     /locus_tag="OO448_003191"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR6338333.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3705..4340
                     /gene="msrQ"
                     /locus_tag="OO448_003192"
     CDS             3705..4340
                     /gene="msrQ"
                     /locus_tag="OO448_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6338334.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4597..5247
                     /gene="zinT"
                     /locus_tag="OO448_003193"
     CDS             4597..5247
                     /gene="zinT"
                     /locus_tag="OO448_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6338335.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5590..6120
                     /locus_tag="OO448_003194"
                     /pseudo
     CDS             5590..6120
                     /locus_tag="OO448_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6690..6779)
                     /locus_tag="OO448_003195"
     tRNA            complement(6690..6779)
                     /locus_tag="OO448_003195"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6743..6745),aa:Ser,seq:cga)
     gene            6873..7670
                     /gene="mtfA"
                     /locus_tag="OO448_003196"
     CDS             6873..7670
                     /gene="mtfA"
                     /locus_tag="OO448_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6338336.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7771..7846
                     /locus_tag="OO448_003197"
     tRNA            7771..7846
                     /locus_tag="OO448_003197"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7804..7806,aa:Asn,seq:gtt)
     gene            8160..14110
                     /gene="yeeJ"
                     /locus_tag="OO448_003198"
                     /pseudo
     CDS             8160..14110
                     /gene="yeeJ"
                     /locus_tag="OO448_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3993 a         3354 c         3169 g         3594 t
ORIGIN      
        1 tactgaagcc ctatgttaat ttctttgcgg taacataatc cggaacacat tatgcttatt
       61 gagatagtga tacgtagcac ttcccccatg taattcggca atcgctttga ctaaagaaag
      121 accaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc tacggaagag
      181 tttttcaggc tcatgaattt tcgttccagg actggcgaca tcaatattaa gatagccgtt
      241 ggtatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc taatggcatt
      301 aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga tttgctgatt
      361 gcactcgacc ttaaagcaaa tctctttctc gtctgaaagg tattcaagat aatccaacaa
      421 attttcgact tccttattga gagaaagcga gtccagtttc accaaaacat tgtttttatc
      481 tgcccgggca agaaacagta tgttctctgt taaccgcgaa atattttcca gctcttcaat
      541 gttaccggca attgtttttt gatattcagc gatacttctg gtttgactga gcgtaacctg
      601 attctgaccc agtaatgcat taatgggcgt tctaagttca tgagcgagat cgtcagcaaa
      661 ttgacttaga cgctcaaaat ctttgactaa agcatgatgc attttattca acgcctgccc
      721 aagaggtttt agttctctcg gtaatgcgct aacctcaaca ggctcccggc tatcgttata
      781 attcagcgct tccgttacac cactcaactt tttgatctct cgtaatcccg ttctgattaa
      841 cagcggactt aatactgagc aaagtacaat ggcgacaatg caaattataa tgctattaat
      901 tttatactgt tcaagcatgt tatgtctggc tgaagccaat ttagccacag taaccgttaa
      961 tgatggcgaa acttcatcaa tattaattcg taaagcatca atctcagtat cattaataat
     1021 gcttctgtaa atgccagctg cgctgattgt ctcactagca ggtatgttat ttaacatgtc
     1081 atcactgaca tttgtccggt taacaatttt attgatgcca tcaccatgaa tgatcaagat
     1141 atcctgacta acatccatca tccggttaaa gtatacaggt aacgtatctg gatttacccc
     1201 atcaattaac aactgcttga tctgtgctgt ccggttaatg agtgttgtat catcgcgcca
     1261 tttcaactca cttgccaggc cattatagag agtccagaca attccagcgc cagcaacaga
     1321 cagcagcaat ataaaaagca aggttaaacg gacggttata gataatcttt tcattatttt
     1381 tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt ttcagaaaaa
     1441 ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt ggtatcacta
     1501 tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat ttcgccagct
     1561 ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat actgatattg
     1621 ttcctgctca cactttgact tacagagtcc attcttaagc cactgatttc taatgttgaa
     1681 ttcaaagcgt gatgttgcct taattgtgcc cgaacccttg ccagcaactc agaaaatgaa
     1741 aaaggtttta ccagataatc atttgctcca ctgtccagcc ctctgactct gtcatcgaca
     1801 gaatcccttg cagtaaggca aataacaggg gtttgctttg ctgttcttaa cgtttgtaag
     1861 atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc ataatcatcc
     1921 ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac ataacccgct
     1981 tcggaaagcc cctgcgttac ccactcctgg gtcctttgat tatcttcaat aagtaaaatc
     2041 ttcatgaaaa taatatgcca taaatcatat gttatagaaa cagcctagtt cattacaaaa
     2101 ttgtaatgct gctgtaaggt taccctggcc gctttttcgc tatcctcaaa actcattcac
     2161 atgacaagga tataaacatg ttaaagcgtt atttagtact ctccgtagta acggcagcat
     2221 tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg cacattttga
     2281 accagcaaac cggaaaaccc gctgccgacg tgacagtcac tcttgaaaag aaagcggaca
     2341 acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt aaggcactgt
     2401 ggcccgagca aactgcaact acgggcgatt accgtgtcgt atttaaaacc ggggactatt
     2461 tcaagaaaca aaatcttgaa agtttcttcc ctgagatccc cgttgaattt catattaata
     2521 aagtgaacga gcattatcat gtgcctttat tacttagcca atatgggtat tcaacctatc
     2581 gtggcagtta atgcataaat atgtaaaagc agatctctgc tactgttagg gaggacactc
     2641 cctgacagat taacagtaaa cggctcttgc tggctaacga caaaaaagtg tgatggctta
     2701 tgaaaaagaa tcaattttta aaagaatcag atgttacggc cgagtcggta ttctttatga
     2761 agcgtcgaca ggtgttaaaa gcactgggca tcagcgcagc tgcactttct ttgcctcacg
     2821 ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgcccgccc gcccccgccg
     2881 gaaaaccgct ggagttcagc aagcctaccg cctggcaaaa taatctgcca ctgacgccag
     2941 tagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa gccgatcccg
     3001 ccgctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc ggcgaagtgg
     3061 caaaaccatt gaccctcgat cacgatgatt taacccgtcg cttcccgctg gaagagcgta
     3121 tttatcgtat gcgctgcgtg gaggcatggt cgatggtggt gccgtggatt ggttttccgc
     3181 tgcacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc gctttcgaaa
     3241 caatttatgc accggaacaa atgccaggcc agcaggaccg ctttatcggc ggcgggctga
     3301 aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc acactgatga
     3361 ccgtgggtgt ttatggcaag gcgttaccgc cacaaaatgg cgcgccggta cgactgattg
     3421 tgccgtggaa atatggcttt aaagggatta aatcgatagt cagtattaag ctgacccgcg
     3481 agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt tacgccaacg
     3541 ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt attggttcag
     3601 gcggcatcct cgatgttcag cgccagccaa cgctactgtt taatggttac gccgaccagg
     3661 tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctgaatgcgt ctgacagcaa
     3721 aacaggtgac atggctgaaa gttagcctgc atcttgccgg attgttgccg tttctctggc
     3781 tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat attcagcatt
     3841 ttaccggtcg cactgcactg aaatttttgc tggcgacctt gttaatcaca cctctggcac
     3901 gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta tggtgctttg
     3961 cctgggcgac actgcattta accagttacg cattgctgga gctgggcgtg aataatctgg
     4021 cgttattagg caaggagtta attacccggc cttatttaac gttaggaatt atcagctggg
     4081 ttattttgct tgctttagcg tttacttcaa cccaggcgat gcagcgaaaa ctcggcaagc
     4141 actggcaaca gttgcataat ttcgtctatc ttgtcgcgat cctggcccca atacattatc
     4201 tgtggtcagt gaagattatt tcaccgcagc cgctcatcta cgctgggctg gctgtactgc
     4261 ttttagcctt acggtataag aagttgcgtt ccctttttaa gcggttacgc aagcaggttc
     4321 acaacaaatt atctgtataa cttcatattg ccgacaaagt acgcgttatc cctgccggat
     4381 gtggcgcgag cgccctgtcc ggcctgcgca gatcacatca ggttatgttt cgtttgtcat
     4441 caacctcaaa ttatcaacat aattcatttt atttcaataa gatagataag tacacctgag
     4501 aaaactatgc tctcatctcc taagagttgt tgcattttgc catatgttac aatataacat
     4561 tacacatcat atacaatgac tctggaggaa actgttttgg caattcgtct tcacaaactg
     4621 gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg tcatcactca
     4681 cacggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt tgatgatgcc
     4741 aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt ttatccttta
     4801 ctgcaaagtg gcaaacttga ccccgtcttt cagaagaaag cggatgcaga taaaattaaa
     4861 acatttgctg aaattaaaga ttattatcac aaaggttatg caacagatat cgagatgatt
     4921 ggcattgagg acggcattgt tgaattccat agaaataatg aaacaacatc ctgtaaatat
     4981 gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt tcgttactta
     5041 ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag cgatcatata
     5101 attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgattc acagcaattt
     5161 ttattaaatg aaatggaaaa ctggccaacg tattatccat atcagttgag tagcgaagaa
     5221 gtggtcgagg aaatgatgtc tcattgagta ttctcatgat aacgcctcga tgccgcttta
     5281 gtaagtcatc ataactgcca ctggtccatc cacaaacgcc actgaacgca agctagctac
     5341 agacacgctc atcactatga cgtgtctgta tattaataag ctaacccgca ttgagttaac
     5401 caataacgga ttccatacac aatacggcca acagagaaag atacctgtgc tcacgccatt
     5461 gcttatattg gctggttaca atgtgcacta tcaatttttt aaataacaaa ttcttaaaac
     5521 cacatctgta taaggaattt ttaaggttcg tgggatagct tgactgtaaa aattacagga
     5581 gctacaaaaa tgaaccgatt ctcaaaaact caaatttatt tacattggat aacgctgctt
     5641 ttcgttgcaa taacctatgc cgcgatggaa ctccgtggct ggtttcctaa aggtagtagt
     5701 acttatctgc tgatgcgaga aacacattac aatgcgggta tattcgtttg ggtgttaatg
     5761 ttttcacgcc tgattataaa acaccgttat agtgatcctt ctattgtacc accgccacct
     5821 gcctggcaaa tgaaagcggc ttcgctaatg cacatcatgc tttatataac cttccttgca
     5881 cttcctctgc tggggattgc tttgatggct tacagtggaa aatcgtggag tttccttggt
     5941 ttcaatgtgt ctccctttgt taccccaaac agcgaaatta aagcactgat aaaaaatatt
     6001 cacgaaacct aggcaaatat aggttacttt ttaatcgcag ctcacgctgg cgcagcactc
     6061 tttcatcact acattcagaa agataatact ctgttacgaa tgatgcatcg ccgcaaataa
     6121 gtcgatcccc tgacaaggaa atgtgaacca tgtacatcaa cgcgtcataa atgtttacgc
     6181 aagtgcacaa tgcatccttt ctactatcaa caaggaggag cagaaactgt gtaaatcgta
     6241 caatggtaag acttatcaaa aatacaggtt aattttggta tttaaacctt ttttacttgt
     6301 tagtgtgata caaaggcttt caaaaaagct gggaaattca acaactcttg gaaattatgg
     6361 actttgtccc gcggctttaa tctgacggcc gcgttccttt tttgactcaa ttattgaccc
     6421 cttctctacg caacttcagt tcccaccacc aactttgcgg cagtttcata ggatgaatgt
     6481 ctaaaagaat aatggtgagc gataagaaaa cgactgaata actgcacatt ttcgctcgaa
     6541 agcttcctgt cagatccata gcgaatcaag tgctgaatgt cacagtatcg aacagaaaac
     6601 agtgacgatc taacccttca agaatattct acaattgttc tgtttaggaa aagcaaggcg
     6661 ggaagtcagg agataagtca ttgataaagt ggcggagaga gggggatttg aacccccggt
     6721 agagttgccc ctactccggt tttcgagacc ggtccgttca gccgctccgg catctctccg
     6781 ttcagatggt tgccatgatg ccaggaaatt tggcatttta acagtccctg ttcgtgcaat
     6841 tttgttcaag tgacgagttt gcgagcaaaa cgatgattaa gtggccctgg aaagtacaag
     6901 aatcagcaca tcaaactgcc cttccctggc aggaagcact atcgatcccc cttttaacgg
     6961 gtctgacgga acaggaacaa agcaaattag tcactcttgc cgaacgtttt ttacagcaaa
     7021 agcggcttgt tcctttacag ggctttgaac tggattcatt aagaagctgc cggatagcac
     7081 ttctattttg cctacccgtt ctggagttag gactggaatg gctggatggt tttcatgaag
     7141 tcttaattta tcctgcgcca tttgtggtcg atgatgaatg ggaagacgat atcggtctgg
     7201 tgcataacca acgtattgtt cagtcaggtc agagctggca gcaagggcct atcgttttga
     7261 actggttgga tatacaagat tcttttgatg catctggttt taacctgatt attcatgaag
     7321 tcgctcataa gctggacacc cgtaacggcg atcgcgccag cggagttccc tttattccgt
     7381 tgcgtgaggt tgctggctgg gaacacgatc ttcatgctgc aatgaacaac attcaggaag
     7441 aaatcgaatt ggttggcgag aatgcggcga gcattgatgc ttatgcagcc agcgatcctg
     7501 ctgaatgttt tgccgtactt tctgaatatt tctttagcgc cccagaactt tttgctcctc
     7561 gtttcccttc attgtggcaa cgtttctgcc aattttatca acaagatcct ttgcagagac
     7621 tgcatcacgc taatgataca gactcgtttt cggcgacgaa tgttcattaa ttaacaactt
     7681 tgcagattaa ttaaccaatt gaaatgactt atgaaattta gtgttgacag acaaggtacc
     7741 gctaagtaat atgtgccccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg
     7801 actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt ctaaaaattc
     7861 gcttttttag cgcaatgtca ctgaccttag ttgaacattg ttttttaacg gatagcgggt
     7921 ttttaacacc ttaagcgccc tcgaccttta tggttgaggg cgttttgcta tgaacgccat
     7981 caccattttc ccctcgatta taaaacttga gttattcagt agtctcccct cttgcaactc
     8041 acacccaaaa ctgcctaacg aaaagttatt aattttcaat cgtattgcta tcagtattta
     8101 cattttttcg ctgtgctaga aagggcgcat ttatgttagc tcgttcaggg aaggtaagca
     8161 tggctacgaa gaagagaagt ggagaagaaa taaatgaccg acaaatatta tgcgggatgg
     8221 gaattaaact acgccgctta actgcgggta tctgtctgat aactcaactt gcgttcccta
     8281 tggctgcggc agcacaaggt gtggtaaacg ccgcaaccca acaaccagtt cctgcacaaa
     8341 ttgccattgc aaatgccaat acggtgccct acacccttgg agcgctggaa tcggcccaaa
     8401 gcgttgccga acgtttcggt atttcggtgg ctgagttacg caaactcaac cagtttcgta
     8461 cgtttgctcg aggttttgat aatgtccgcc agggtgatga actggatgtc ccggcacaag
     8521 ttagtgaaaa aaaattaacc ccgccgccgg gtaataacag tgacaacctc gagcaacaga
     8581 tagccagtac ttcacagcaa atcgggtctc tgctcgccga agatatgaac agcgagcaag
     8641 cggcaaatat ggcgcgtgga tgggcctctt ctcaggcttc aggcgcaatg acagactggt
     8701 taagccgctt cggtaccgca agaatcacgc tgggcgtgga tgaagatttt agcctgaaga
     8761 actcccagtt cgattttctc catccgtggt atgaaacgcc tgataatctc tttttcagtc
     8821 agcatactct ccatcgtact gacgagcgta cgcagattaa caacggctta ggttggcgtc
     8881 atttcactcc cacatggatg tcgggcatca acttcttttt cgaccacgat cttagccgtt
     8941 accactcccg cgtcggcatt ggcgcggagt actggcgcga ctatctaaaa ttaagcagta
     9001 acggctattt gcgactgacc aactggcgca gcgcacctga actggacaac gattataaag
     9061 cacgcccggc caatggctgg gatgtacgcg cagaaagctg gctacccgcc tggccgcacc
     9121 ttggcggtaa actggtctat gaacagtatt atggcgatta agtggccctg ttcgataaag
     9181 acgatcggca aagtaatcct catgccataa ccgctggact taactatacc cccttcccgc
     9241 tgatgacctt cagcgcggag caacgccagg gtaaacaggg cgaaaatgac acccgttttg
     9301 ccgtcgattt tacctggcaa cctggcagcg caatgcagaa acagcttgac ccgaatgaag
     9361 tcgctgcacg gcgtagcctt gcaggcagcc gttatgatct ggtggatcgc aacaacaata
     9421 tcgttctgga atatcgcaaa aaagaactgg ttcgcctaac cctgacagac cccgtgacag
     9481 ggaagtcagg agaagtgaaa tcactggttt cgtcgctaca aaccaaatat gccctgaaag
     9541 gctataacgt cgaagccacc gcactggaag ctgccggtgg caaagtggtc acaacgggta
     9601 aagatattct agttaccctg ccggcttacc ggttcaccag tacgccagaa accgataaca
     9661 cctggccgat tgaagtcacc gccgaagatg tcaaaggcaa tttgtcgaat cgtgaacaga
     9721 gcatggtggt cgttcaggca cctacgctaa gccagaaaga ttcctcggta tcgttaagta
     9781 cccaaacatt gaacgcggat tcccattcaa ccgccacact gacttttatt gcgcatgatg
     9841 cagcaggtaa tcctgttgtc gggctggtgc tctcgacgcg tcacgaaggt gttcaggaca
     9901 tcaccctttc tgactggaaa gataatggtg acggaagcta tacccagatc ctgaccacag
     9961 gagcgatgtc tggcacgctg acgctgatgc cacagctgaa tggtgtggat gcggctaaag
    10021 cccccgccgt ggtgaatatc atttctgttt cgtcatcccg aactcactcg tcaattaaga
    10081 ttgataagga ccgttatctc tccggcaatc ctatcgaggt gacggtagaa ctgagagatg
    10141 aaaatgacaa acctgttaag gaacaaaaac agcaactgaa taacgcagtc agcatcgaca
    10201 acgtgaaacc aggagtcact acagactgga aagaaaccgc agatggcgtc tataaggcga
    10261 cctataccgc ctataccaaa ggcagtggac ttactgcgaa gctattggaa tgaagatttg
    10321 cataccgctg gatttatcat cgacgccaac ccgcagtcag cgaaaattgc gacattatct
    10381 gccagcaata atggtgtact cgccaatgag aatgcagcaa acaccgtctc ggtcaatgtc
    10441 gctgatgaag gaagcaaccc aatcaatgat cataccgtca cgtttgcggt attaagcgga
    10501 tcggcaactt ccttcaacaa tcaaaacacc gcaaaaacgg atgttaatgg tctggcgact
    10561 tttgatctga aaagtagtaa gcaggaagac aacacggttg aagtcaccct tgaaaatggc
    10621 gtgaaacaaa cgttaatcgt cagttttgtc ggcgactcga gtaccgcgca ggttgatctg
    10681 cagaagtcga aaaatgaagt ggttgctgac ggcaatgaca gcgccacaat gaccgcgacc
    10741 gtccgggatg caaaaggcaa cctgctcaat gacgtcatgg tcactttcaa tgttaattca
    10801 gcagaggcga aactgagcca aaccgaagtg aatagccacg acgggatcgc cacagctacg
    10861 ctgaccagtt tgaaaaatgg tgattatagg gttacggcct ctgtgagctc tggttcccag
    10921 gctaatcaac aggtgaattt tatcggtgat caaagtactg ctgccctgac cctcagtgtg
    10981 ccttcaggtg atatcaccgt caccaacaca gctccgcaac atatgactgc aaccttgcag
    11041 gataaaaatg gcaacccact aaaagataaa gaaatcacct tctctgtgcc aaacgacgtc
    11101 gcaagtaagt tctcgattag caacggagga aaaggcatga cggatagtaa cggggttgca
    11161 atcgcctccc tgaccggcac gttagcgggc acgcatatga tcatggctcg tctggctaac
    11221 agcaatgtca gcgatgcaca gccaatgacg tttgtggcgg ataaagacag agcggttgtc
    11281 gttttgcaaa catcgaaagc ggaaatcatt gggaatggcg tggatgagac aactctgaca
    11341 gcaacagtga aagatccgtc gaatcatccg gtggcgggga taacggtaaa cttcaccatg
    11401 ccacaggacg ttgcggcaaa ctttaccctt gaaaataacg gtattgccat cactcaggcc
    11461 aatggggaag cgcatgtcac gctgaaaggt aaaaaagcgg gcacgcatac ggttaccgca
    11521 acgctgggta ataacaatac cagtgattcg cagccggtaa catttgtggc ggacaaagcc
    11581 tcggctcagg ttgtcctgca gatatcaaaa gatgagatca caggtaatgg cgtcgatagc
    11641 gcaacgctaa ctgcaacggt taaagatcag ttcgacaatg aggtgaataa tcttccggta
    11701 acattcagct cagcctcttc aggactcacc ctgaccccgg gagtaagtaa taccaatgag
    11761 tctggcatcg cgcaggccac tctcgcaggc gttgcctttg gtgagcagac ggtcactgca
    11821 tcactggcta ataatggtgc cagcgacaac aaaactgtgc attttattgg cgacacagcg
    11881 gcggcaaaaa ttatcgagtt gacgcctgtc ccagacagca taatcgccgg taccccgcag
    11941 aacagctccg gcagcgccat caccgccaca gtcgttgata ataatggctt tccggtgaaa
    12001 ggtgtgactg tgaacttcac cagcagaaca aactctgccg aaatgacgaa tggcggccaa
    12061 gccgtaacga acgaacaggg taaggctacc gtcacttata ccaatacccg ctcctcgata
    12121 gaatcaggag cgagaccgga taccgttgag gccagtctgg aaaatggtag ctccacgctt
    12181 agcacatcaa ttaatgtcaa cgctgatgcg tctacggcac atctcacctg gctacaggca
    12241 ctttttgata cagtctccgc aggcgagaca accagtctgt atattgaggt gaaggataat
    12301 tacggcaacg gtgtccccca gcatcaggtg accctcagcg tttcaccaag tgaaggcgtg
    12361 acccccagta ataacgctat atatactacc aaccacgacg gcaattttta cgcaagcttt
    12421 accgctacaa aagccggggt ttatcaagtg acggcaaccc tcgaaaatgg cgattcgatg
    12481 caacaaacag tgacctatgt gccgaacgtc acgaatgccg aaatcacgct ggcaacctcg
    12541 aaggatccgg tgattgccga caataacgat ctcacgacac taacagcaac agtcgctgat
    12601 acagagggca atgcgatagc caacactgaa gtaacattta ctctgccgga agatgttaag
    12661 gcgaacttca cgctgagcga tggcggtaaa gcgattactg atgctgaagg caaagcgaaa
    12721 gtcacgctga aaggtacaaa agcaggcgct catactgtta cagcatcgat aactggcggt
    12781 aagagtgagc agttggtggt gaactttact gcggatacgc tcactgcgca ggttaatctt
    12841 aacgttaccg aggacaattt tatcgctaat aacgtcggga tgaccaggct gcaggcaaca
    12901 gtgactgatg gaaacggcaa cccgttagcc aatgaggcgg tgacattcac gctaccggca
    12961 gatgtgagcg caagctttac tctcggacaa ggcggttccg ccattactga catcaacggc
    13021 aaggctgaag ttacactgag cggtacaaaa tccggcacct accccgtgac agttagcgtg
    13081 aacaattatg gtgtcagtga tacgaaacag gtgactttga ttgccgatgc tggtaccgca
    13141 acactagcct ccttaacctc tgtatactca ttcgtcgtca gcacgaccga aggcgcgacc
    13201 atgactgcaa gcgtcactga cgctaacggc aacccggtag aaggcataaa agttaatttc
    13261 cgcggaacct ccgtcacgct aagcagcacc agcgttgaaa cggatgatcg gggtttcact
    13321 gaaattcttg tgacaagcac cgaagtcgga ctgaaaacag tttcagcctc tctggcagat
    13381 aaacctactg aagtcatatc gcgattactg aatgcaaaag cagatattaa ttctgcaacg
    13441 attaccagtc tggagatacc tgaaggtcag gtcatggtcg cacaagacgt agcagttaaa
    13501 gctcacgtca acgaccagtt tggcaacccg attcttaatg aatctgtaac attcagtgca
    13561 gagccaccag agcacatgac catcagccaa aatattgtct ctactgatac gcatggtata
    13621 gccgaggtct ccatgacgcc cgaaagaaac ggttcgtata tggtgaaagc atccctggcg
    13681 aatggagcct cacttgagaa acaactggag gctattgatg aaaaactgac actctcggcg
    13741 tccagcccgc ttatcggtgt caattcccca acaggtgcaa ctctgacggc aacgctaact
    13801 tctgcaaatg gcactccagt ggagggtcag gtcatcaact ttagcgtaac gccagaaggt
    13861 gcgacgttaa gtggcggaaa agtgagaact aactcttctg gtcaggctcc agtcgttctg
    13921 accagcaata aagtcggtac atatacggtg actgcatcgt tccataacgg cgtaacaata
    13981 cagacacaga caaccgtgaa agtcactggc aactcaagca ccgcccatgt tactagcttt
    14041 atcgctgatc catcgactat cgccgccacc aacagtgatt taagtacctt aaaggcaacg
    14101 gttgaggatg
//
LOCUS       DAJTVJ010000110        13905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000110 DAJTVJ010000000
VERSION     DAJTVJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.110"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="OO448_003199"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="OO448_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6338337.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="OO448_003200"
     CDS             complement(1550..1723)
                     /locus_tag="OO448_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6338338.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="OO448_003201"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="OO448_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6338339.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="OO448_003202"
     CDS             3361..3690
                     /locus_tag="OO448_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6338340.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OO448_003203"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OO448_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6338341.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OO448_003204"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OO448_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6338342.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OO448_003205"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OO448_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6338343.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OO448_003206"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OO448_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6338344.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OO448_003207"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OO448_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6338345.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9613..9909
                     /locus_tag="OO448_003208"
     CDS             9613..9909
                     /locus_tag="OO448_003208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338346.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OO448_003209"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OO448_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6338347.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="OO448_003210"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="OO448_003210"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6338348.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3437 c         3231 g         3579 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttctg
//
LOCUS       DAJTVJ010000111        13874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000111 DAJTVJ010000000
VERSION     DAJTVJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.111"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            3..2126
                     /gene="ldcC"
                     /locus_tag="OO448_003211"
     CDS             3..2126
                     /gene="ldcC"
                     /locus_tag="OO448_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6338349.1"
                     /translation="MGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLKFIEHNP
                     RICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWFFEYALG
                     QAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQKSPVGCL
                     FYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTNGTSTSN
                     KIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIPRREFTR
                     DSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWVPYTHFH
                     PIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEAFMMHTT
                     TSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWFFDIWQP
                     PQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEGIPAALV
                     AKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRIKNMLPD
                     LYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAWQRQIKG
                     EVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSVGQHYPG
                     FETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2183..2572
                     /locus_tag="OO448_003212"
     CDS             2183..2572
                     /locus_tag="OO448_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6338350.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2637..3935
                     /gene="tilS"
                     /locus_tag="OO448_003213"
     CDS             2637..3935
                     /gene="tilS"
                     /locus_tag="OO448_003213"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6338351.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(3984..4244)
                     /gene="rof"
                     /locus_tag="OO448_003214"
     CDS             complement(3984..4244)
                     /gene="rof"
                     /locus_tag="OO448_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6338352.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4231..4431)
                     /locus_tag="OO448_003215"
     CDS             complement(4231..4431)
                     /locus_tag="OO448_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6338353.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4597..5142
                     /locus_tag="OO448_003216"
     CDS             4597..5142
                     /locus_tag="OO448_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6338354.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5139..5500
                     /gene="arfB"
                     /locus_tag="OO448_003217"
                     /pseudo
     CDS             5139..5500
                     /gene="arfB"
                     /locus_tag="OO448_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5574..6284
                     /gene="nlpE"
                     /locus_tag="OO448_003218"
                     /gene_synonym="cutF"
     CDS             5574..6284
                     /gene="nlpE"
                     /locus_tag="OO448_003218"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6338355.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6439..7263)
                     /locus_tag="OO448_003219"
     CDS             complement(6439..7263)
                     /locus_tag="OO448_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6338356.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7316..9034)
                     /gene="proS"
                     /locus_tag="OO448_003220"
     CDS             complement(7316..9034)
                     /gene="proS"
                     /locus_tag="OO448_003220"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6338357.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9145..9852)
                     /gene="tsaA"
                     /locus_tag="OO448_003221"
     CDS             complement(9145..9852)
                     /gene="tsaA"
                     /locus_tag="OO448_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6338358.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9849..10253)
                     /gene="rcsF"
                     /locus_tag="OO448_003222"
     CDS             complement(9849..10253)
                     /gene="rcsF"
                     /locus_tag="OO448_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6338359.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10371..11186)
                     /gene="metQ"
                     /locus_tag="OO448_003223"
     CDS             complement(10371..11186)
                     /gene="metQ"
                     /locus_tag="OO448_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6338360.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11226..11879)
                     /gene="metI"
                     /locus_tag="OO448_003224"
     CDS             complement(11226..11879)
                     /gene="metI"
                     /locus_tag="OO448_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6338361.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11872..12903)
                     /gene="metN"
                     /locus_tag="OO448_003225"
     CDS             complement(11872..12903)
                     /gene="metN"
                     /locus_tag="OO448_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6338362.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13091..13666
                     /gene="gmhB"
                     /locus_tag="OO448_003226"
     CDS             13091..13666
                     /gene="gmhB"
                     /locus_tag="OO448_003226"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6338363.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3367 a         3515 c         3690 g         3302 t
ORIGIN      
        1 ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg gagtcggcgc
       61 tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg ctgaaattta
      121 tcgaacataa ccctcgaatt tgtggcgtga tttttgactg ggatgagtac agtctcgatt
      181 tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc atcaacaccc
      241 actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt tttgaatatg
      301 cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac gaatatcttg
      361 ataacattac gccgccgttc acgaaagcct tgtttaccta cgtcaaagag cggaagtaca
      421 ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg gttggctgcc
      481 tgttttatga ttttttcggc gggaataccc tcaaggctga tgtctctatt tcggtcaccg
      541 agcttggttc gttgctcgac cacaccgggc cacacctgga agcggaagag tacatcgcgc
      601 ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg tcgaacaaaa
      661 ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc aattgtcata
      721 aatcgctggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg aaaccgacgc
      781 gtaatgcgtt ggggattctt ggtgggatcc cgcgccgtga atttactcgc gacagcatcg
      841 aagagaaagt cgctgccacc acgcaggcac aatggccggt tcatgcggtg attaccaact
      901 ccacctatga tggcttgctc tacaacaccg actggatcaa acagacgctg gatgtcccgt
      961 cgatccactt cgattccgcc tgggtgccgt acacccattt tcatccgatc taccagggga
     1021 aaagtggtat gagcggcgag cgtgttgcgg gaaaagtgat cttcgaaacg caatcgaccc
     1081 acaaaatgct ggcggcgtta tcgcaggctt cgctgatcca cattaaaggc gagtatgacg
     1141 aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt tatcccattg
     1201 ttgcttcggt tgagatggcg gcggcgatgc tgcgtggtaa tccgggcaaa cggctgatta
     1261 accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggttg cgggaagagt
     1321 ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc gaatgctggc
     1381 ccgttgcgcc aggcgaacag tggcacggct ttaacgatgc ggatgccgat catatgtttc
     1441 tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc aatatgagcg
     1501 aggaggggat cccggcggcg ctggtggcaa aattcctcga cgaacgtggg atcgtagtag
     1561 agaaaaccgg cccttataac ctgctgtttc tctttagtat tggtatcgat aaaaccaaag
     1621 caatgggatt attgcgtggg ttgacggaat ttaaacgctc ttacgatctc aacctgcgga
     1681 tcaaaaatat gctacccgat ctctatgctg aagatcccga tttctaccgc aatatgcgta
     1741 ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt cccggtttga
     1801 tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag gcatggcaga
     1861 gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt agagtatcgg
     1921 caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga gaaatgctga
     1981 ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta gggcaacatt
     2041 accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc gtttaccgcg
     2101 tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga gtaacgtgct
     2161 gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata ttgcgattat
     2221 tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt tcacgctgca
     2281 aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc ttaatgggca
     2341 atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc gaccggaagc
     2401 ttgcggcctg cgtcatctgg cttttagcgt tgatgatatc gatgcggcag tggcgcacct
     2461 tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc aaaaacgctt
     2521 cacgttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt aaggcttgtc
     2581 atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc accatcatga
     2641 cactcacgct caatagacaa cttctcacct cacgccagat tctggtggcc tttagcggcg
     2701 ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa aatccgggcg
     2761 tcactctacg cgctatccat gtgcatcatg gtttaagtgc taatgccgat gcctgggtta
     2821 ggcattgcga gaacatctgc caacagtggc aggtgccgct ggtggtcgag cgcgtgcaac
     2881 ttgcgcaaga aggactgggc attgaggccc aggcgcggca ggcgcgttat caggcatttg
     2941 ctcgtaccct gttacccggt gaagtgctgg tcaccgcgca acatctcgac gatcaatgtg
     3001 aaacctttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg gctatggggg
     3061 aagtttcgga atttgccgga acacggctta tccgcccgtt gcttgcccgc acgcgggggg
     3121 aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa agtaatcagg
     3181 acgactcata cgatcgtaac tttctgcgcc tgcgtgtagt gccgttattg cagcagcgtt
     3241 ggccgcattt tgccgaagca acggcccgca gcgccgcact ttgtgctgaa caagagagtc
     3301 tgttggatga actgctggcg gatgatttag cacactgcca aacgtcgcag gggacgctgc
     3361 agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatc cgccgctggc
     3421 tggcagggca gaatgcaccg atgccttccc gcgacgcgct ggtgcggatc tggcaggaag
     3481 tggcgctggc gcgggaagat gcctccccct gtttacgttt tggcgcgttt gaaatacggc
     3541 gctatcagtc gcaactgtgg tggattaaat ccgtcactgg gcaaagcgaa accattgtgc
     3601 cgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaagt gtacagctta
     3661 ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg cgtttcaaag
     3721 cgccaggatt gctgcatatt gtcgggcgta acggcggacg taagctaaag aaaatctggc
     3781 aagagctggg cgtgccgccg tggctacgtg acaccacgcc actgctgttt tatggcgaaa
     3841 cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa ggtgagaatg
     3901 gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa gacgcatcaa
     3961 acgtcgcatc cggcgaaagt caatcaggat tcgcttacca ccaccgtacc gatttccggg
     4021 tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc ggcctcgacg
     4081 accagatact ccacattttt gcgggagact aaatcactgg cttttgcctg caatttttcg
     4141 ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc aagattatcg
     4201 taatcatcac aattgattgg ttgatacgca tcattcattg acataatcgc tcaccagtaa
     4261 gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt cgtcggcagc
     4321 catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc ctaagtctcc
     4381 gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca catgccctcc
     4441 tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata aactctgttt
     4501 cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg cacgtattcc
     4561 gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc gacaatttat
     4621 aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc ctctctgacg
     4681 ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcctgct ggcgtggctg
     4741 aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatta tgagccggaa
     4801 gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg gctgccggat
     4861 gagcggcgga ttaagaaagc ctgcacccag gccgcaaaag tggcgctgtt tacctataat
     4921 agccgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca gtttgccaat
     4981 ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt tgccgatcgt
     5041 accatgacgc tgcaggcaac gattcaggat ggcgtgatct ggttatcgga tgataagaat
     5101 aatctggaag tgaacttaac cgcctggcaa caaccttcat gattgtgatt tcccgacatg
     5161 ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc gcgggcgggc
     5221 agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg gcgtccagcc
     5281 tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc agcatgatgg
     5341 cgtaattgtc attaaggcac aggaataccg cagtcaggaa ctgaaccgcg aagcggcgct
     5401 ggcccggctg gtggcagtga ttaaagattt aacaacagaa caaaaagccc gacgacccac
     5461 gcggcccacc cgtgcatcga aagagcgcag gctggcatag aaagcacaaa aatcaagcgt
     5521 gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa aaagaaggaa tggatggtga
     5581 aaaaagcgat agtgacagcg atggctgtaa tcagcctctt tactctgatg ggatgtaata
     5641 atcgggccga agtcgatacg ctttctccgg cgcaggctgc cgaactaaaa ccgatgccgc
     5701 aaagttggcg cggcgtgctg ccgtgtgccg attgcgaagg gatcgaaacc tctctgttcc
     5761 tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct cggtgctcgt gaagaacctt
     5821 cctccttcgc ttcctacggt acatgggcgc gaaccgctga caagctggta ttaaccgaca
     5881 gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc gctggagatg ctcgatcgtg
     5941 aaggcaatcc aattgaatcg cagttcaact atacgctgga accggcacaa tccagtttac
     6001 ctatgacgcc gatgaccctg cgaggcatgt atttttatat ggctgatgcg gcgaccttca
     6061 ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa cgcagagctg gagcgtagct
     6121 acctggctgc gcgcggtcac agtgaaaaac cggtgttact gtcagtagaa ggtcacttta
     6181 cgcttgaggc taatccggat actggtgcgc caaccaaagt attagcgccc gatacggcgg
     6241 gtaaatttta cccgaataag gattgcagta gtttggggca gtaaacctgc ttaaggcaga
     6301 aacaaacgca aaattgcctg atgcgctgcg cttatcaggc ctacagcata taatgcaaca
     6361 ttttgaatta gcacgatttt gtaggccgga taaggcgttc acgacgcatc cggcattttg
     6421 tgcacgaggc tggctttatc actgagtcag cccaacaaag cgacccgcct tgatataaat
     6481 ccctggcttc aggtgcccaa catactgtaa ctgcgtttct ggcttaaacg cagagacatc
     6541 gccagtacgc atatgcatca gatcggcagg gctaatccag cctgattgcg ccagcgtaag
     6601 cggatgcccg gctttggcaa atgcatcagt gacaaattcc gaacaaaacc acgacttttt
     6661 gtctccttcg cccacactgc ttaactgcgc tttcgccagg ccgctgacgc actgttggcg
     6721 aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa agggaataaa
     6781 ttcgacaatg ccgcgatagt tataactgct atctttgatt ttattggcaa acgcggtgat
     6841 atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcactatg
     6901 cttcatcgct tttttaaggg aaacgatctg gacgccagcg cctgtcgctt ctgcaacgtt
     6961 attatcaccc agatagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
     7021 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtac
     7081 cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt tgacagccca
     7141 tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga tatcaacggt
     7201 acaagccgaa agtaaaagaa agctggggag aagcaggcgg cagtacgcct ttggtttatc
     7261 cattttatac aatccatgta aaaaagggcc ctgaaattca ggaccctttc tggcatcagc
     7321 ctttaatctg tttcaccaga tattcgacga tgtcaccagt cttaattaac tgtttctcgc
     7381 cgttgcgacg atatttatat tcgatatcgt cgttgtcgag gttacggtcg cccagcacaa
     7441 tagtgtgcgg aataccgatc agttccatat cagcaaacat cacgcccgga cgctctttgc
     7501 ggtcatccag cagcacttcg ataccttgtg cacgcagttc gctgtacagt ttctcagcaa
     7561 gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag aatcgccacc tggaacggcg
     7621 cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc gccgcagcta
     7681 ccacacgcgt taccccgata ccgtagcaac ccatcgtcag gatttggtta cggccatctt
     7741 caccctgtac ggaggctttc agtgcttcgg agtacttggt acccagctgg aagatgtgac
     7801 caacttcgat accacgtttg atcagcagcg taccctggcc atccgggctt ggatcgccag
     7861 ccaccacgtt acggatatct gcaacttccg gggtagcgac atcgcgatcc cagttgatac
     7921 cgaagtagtg tttaccatcg atgttagcac cagcagcgaa atcactcatc gccgcaacgg
     7981 tacggtcaat caccaccgga atcggcatgt ttaccggacc cagtgaaccc ggaccggctt
     8041 taaccacagc acgaatttct tcttcggtcg cgaaagtcag cgggcttgca acctgcggca
     8101 gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg caccagcagc gcaaccagcg
     8161 ggaagctgct gccttcaacc gctttaacca gcagagtctt aaccgttttc tcaatcggca
     8221 gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca
     8281 tttcctgggt agcagcagcg cgcggttctt tcggcgcgat agcttctgcc agttcaatgt
     8341 tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca
     8401 cctggaattc gtgagaggcg ctgccgccga tagaaccggt gtcggcttgc acggcgcgga
     8461 aatccagccc catgcggctg aagattttgc tgtaggccgc atacattgca tcgtaggttt
     8521 cctgcaggga ttcctgagaa gtatggaaag agtaagcatc tttcatcagg aattcgcggg
     8581 aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa cttggtctgg atctgataga
     8641 agttcagcgg cagctgtttg taagagctaa gctcgttacg aatcaggtca gtgataactt
     8701 cttcatgagt tgggccgagt acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt
     8761 ccggaccgta ctgttcccaa cgaccactct cttgccacaa atcggctggc tgaaccaccg
     8821 gcatcgacac ctcgattgca ccggcgttgt tcatctcttc acgcacgatg ttttcgactt
     8881 ttttcagaac gcgcacgccg gtcggcagcc aggtatataa cccggaggcc agcttgcgga
     8941 tcatcccggc gcgcagcatc agctgatggc tgatcacctc ggcgtcggca ggtgtctcct
     9001 tgagagtgga gagcaggtat tggctagtac gcatgttgtt acggttccag ttggaaggta
     9061 gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt agtttaccag tgcaaaagaa
     9121 atgtcaaaag agaagggcgt gaatttaacg cggttccagc gcaaagactt caaaacctgc
     9181 gtcggtgacg cgccagcgaa cgttaaaatc atgtagccag acggcatagg ttttgcccgt
     9241 ttcctcacct ttacgatagg ccgggcgcgg gtcctgcgcc agtacttcgc ggataaacag
     9301 cgttaactgc ggataacgct tctccagcgt caaaagctgc ttttcgacct ctgcggtaaa
     9361 actcaccgcc atctctgcag ctggcgcact ttgcgcatag ctggcactgg catcgggaag
     9421 cgattcggca aaggggagat acggtttgat atccactacc ggcgtaccat cgaccagatc
     9481 cagactgccg agcttcagaa tcacgctgtc tttatggcaa acaacctctt tcagctctac
     9541 cagcgacata ccaattgggt tagggcggaa agtagagcgt gttgcgaaaa cccccattct
     9601 ggcgttaccg ccgaggcgcg gtggacgcac agtcggacgc cagccgcctt ccatcgtttg
     9661 atgaaagacg aaaaggatcc ataaatggct gaacgcttcc aggccgcgaa cggcgtcggc
     9721 ctggttgtag ggagcaatga gatgcagttc gccgttggcg ctttttacca gaccaggctg
     9781 gcgcggaacg gcgaattttt ctttataggg cgagcgaata acgcctattt gctcgaactg
     9841 gaaactgctc atttcgccgt aatgttaagc gcagagccga tacatacagc ctgacgatag
     9901 cagcctagcg taccgctggt gacttcgcag ctatgcagta ataccgcatt ggctttcatt
     9961 ttagaggcgt tgatttgcat ccgcttacgc gcggttggaa tgctcggcgg agagtcctga
    10021 ttagaggcct ggcaagagtc gccactgact tcaccgagat cgcggaacgg tttgccgact
    10081 aattcttctg cattggtata aattcggacc ggcgtggcgc gcggcgcttt cggttttgca
    10141 ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt cgacagggga tctgcttaac
    10201 atggaacagc cgcttagcat gagtgctact aaacagatcg gtaaagcacg catagtattt
    10261 cctcaatgta tgatcaaaac gtcaatattg aatcaggagc ttgtaaaaat gacaagacgg
    10321 gcaagcgccc gtcctgaatg atattacaaa ttgtggaaac agcctaaaaa ttaccagcct
    10381 ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt aaacttcatc agactgataa
    10441 gcctggacga atttcttcac gttctcggcg tctttgttat cttcacgcgt tacgatcagg
    10501 ttcacgtacg gggagtcttt atcttcgaca aagataccgt ctttcgctgg agtcaggcca
    10561 atctggctgg cataggtggt attgataact gccagagcga tttgcgcgtc gtccagagat
    10621 cgaggcagct gcggtgcttc cagctcaaca attttcagat tttttgggtt ctcaacaaca
    10681 tcaagaacgg tcggcagcag gccaacgcca tctttcagtt tgatcaagcc cactttttgc
    10741 agcagcagca gtgaacgacc aagattagtt ggatcgtttg gcacggcaac ctgcgaacca
    10801 tcctgcagtt catccagtga tttgattttc ttggagtaac cagcaatcgg ataaacaaaa
    10861 gtgttgccta ctgcgaccag tttgtagcca cgatctttca gttgctgatc aaggtacggt
    10921 ttatgctgga aggcgttggc gtcgatatcg cctttgctca atgcttcgtt tggcagaaca
    10981 tagtcgttga aggttaccag ctcaacgtcc aggccatatt tgtctttcgc aactttctgc
    11041 gcgacttctg ctacctgctg ttcggcacca acaatcacgc cgactttaat gtggtttgga
    11101 tctttttcat cctgaccgca gcctaccagt gccagtgatc caatcagggc tcccactgcc
    11161 gcaaaggttt tgaatttgaa cgccatacct tattccttct tcaattattt atgttgtgtt
    11221 gaacgttact tgcgagtgac agcccggacg atgcggtcgc ctgcgaactg aattaaataa
    11281 accagaatga ccagcaatac cagtaccgta ttcatcaccg tcgcgttgta gccgatgtag
    11341 ccatactgat agccaatctg acctaaacca ccggcaccga ctgcaccacc catcgcggaa
    11401 taaccgacca gggtaatcag ggtgatagtt gccgcattca ccagacccgg caacgcttcc
    11461 ggtaacagca ctttacggac gatctgcatt ggcgtggcac ccattgcgcg ggaagcttca
    11521 attaacccgg ttgggatctc cagcagagcg ttctcgacca tacgggcaat aaacggtgct
    11581 gcaccaacgg ttaacggaac aattgctgcc tgcaaaccaa tcgatgtacc gacaataacg
    11641 cgggtaaacg gaatcatcca taccagcaag ataatgaacg ggatggaacg gaaaatgttc
    11701 acaatcgcag aaacggtacg atacagcttc gcgttagcaa taatttgccc cggacgcgtg
    11761 acataaagca gaacgccaac cggcagacca atcacaaagc caaaaaatcc ggatacgaag
    11821 gtcattgcca gcgtttccca tacgccacga accagcagcc acatcatcgg ctcagacata
    11881 acccagtacc tctactttta catggtgttc ctgcagccag gcaatggcgg cttgcgtatc
    11941 ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaat ttaacgccac cggcgtagtc
    12001 catctgcgcg ctaataatgt tgttgttgac gttgaaacga cgcgcggttt cagaaagcag
    12061 tggggcatcg accgattgac cggtaaactc cagacgcagc atcggcacgc agtcagtaaa
    12121 tggctccgct tgcagacgtt cctggtaatc ttccgggata tccagatgca gggtcgactg
    12181 aataaacttc tgcgccagcg gcgttttcgg atgcgagaac acttcactta ccgtgtcctg
    12241 ctcgatcagt tcgccattgc tgatgaccgc cacgcaatca caaatacgct tcacaacgtc
    12301 catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg cggttaatgt ctttcagcag
    12361 ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg gtggcttcat cacacagcaa
    12421 tactttggga ttgctggcta atgcacgggc aatcgccaca cgttgtttct gcccaccgga
    12481 aagattcgac gggtagctat catgcttatc gccaagacca accaatgaca gcaattccgt
    12541 cacgcgacgt ttgatctcgt ctttcggtgt gttgtccagc tccagcggca gagccacgtt
    12601 gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg aaaatcatac caatctggcg
    12661 gcgagctttg gtcaactcgg attctgacag cgtggtcaat tcttggccat cgaccagcac
    12721 gctaccctcg gttgggcgct ccagcaggtt tacacaacgt ataagcgtac tcttacccgc
    12781 gcctgaggca ccgataacgc cataaatttg tccagctggc acatgcaggc tgacgttgtt
    12841 caacgcctgg atggtgcggg tgccctggtg gaacactttg gtgatattcg aaagttttat
    12901 cattgattat ttattatcgt cattaagtta gtcgtggcat ctcgaatgcc tgaaacgggc
    12961 aacgccgtca atgaaatgga tgttaaggca tccagacgtc taaatcaatc aggtttatgc
    13021 gaagagcact ttcttgcagg tcgaaacatg cgatactagc gtcccatgcc ttattaagga
    13081 gctataaaag gtggcgaaga gcgtacccgc aatttttctt gaccgtgatg gcaccattaa
    13141 tgtcgatcac ggctatgtcc atgagatcga caactttgaa tttatcgacg gtgttattga
    13201 cgccatgcgc gagctaaaaa aaatgggctt tgcgctggtg gtagtaacca accagtctgg
    13261 cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg ctgaccgagt ggatggactg
    13321 gtcgctggcg gaccgagatg tcgatctgga tggtatctat tattgcccgc atcatccgca
    13381 gggtagtgtt gaagagtttc gccaggtctg cgattgccgc aaaccacatc cggggatgct
    13441 tttgtcagca cgcgattatt tgcatattga tatggccgct tcttatatgg tgggcgataa
    13501 attagaagat atgcaggcag cggttgcggc gaacgtggga acaaaagtgc tggtgcgtac
    13561 gggtaaacct attacacctg aagcagaaaa cgcggcagat tgggtgttaa atagcctggc
    13621 agacctgccg caagcgataa aaaagcagca aaaaccggcg caatgattaa aagatgagcg
    13681 gttgaaataa aaatgcattt ttccgcttgt cttcctgagc cgactcccta taatgcgcct
    13741 ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa
    13801 aatcctgaaa ttcagggttg actctgaaag aggaaagcgt aatatacgcc acctcgcgac
    13861 agtgagctga aagc
//
LOCUS       DAJTVJ010000112        13795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000112 DAJTVJ010000000
VERSION     DAJTVJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.112"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..147)
                     /locus_tag="OO448_003227"
     CDS             complement(<1..147)
                     /locus_tag="OO448_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6338364.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="OO448_003228"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="OO448_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="OO448_003229"
                     /pseudo
     CDS             476..1021
                     /locus_tag="OO448_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="OO448_003230"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="OO448_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6338365.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="OO448_003231"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="OO448_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6338366.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="OO448_003232"
     CDS             3028..3201
                     /locus_tag="OO448_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338367.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="OO448_003233"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="OO448_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6338368.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="OO448_003234"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="OO448_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6338369.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="OO448_003235"
     CDS             complement(5499..6422)
                     /locus_tag="OO448_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6338370.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="OO448_003236"
     CDS             6639..7982
                     /locus_tag="OO448_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6338371.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="OO448_003237"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="OO448_003237"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6338372.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYALKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="OO448_003238"
     CDS             10002..10226
                     /locus_tag="OO448_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6338373.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="OO448_003239"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="OO448_003239"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6338374.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /locus_tag="OO448_003240"
     CDS             complement(10820..11800)
                     /locus_tag="OO448_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6338375.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="OO448_003241"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="OO448_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6338376.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="OO448_003242"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="OO448_003242"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6338377.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3482 g         3576 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gctcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catct
//
LOCUS       DAJTVJ010000113        13768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000113 DAJTVJ010000000
VERSION     DAJTVJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.113"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..720)
                     /gene="dsbG"
                     /locus_tag="OO448_003243"
     CDS             complement(<1..720)
                     /gene="dsbG"
                     /locus_tag="OO448_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6338378.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKT"
     gene            1092..1655
                     /gene="ahpC"
                     /locus_tag="OO448_003244"
     CDS             1092..1655
                     /gene="ahpC"
                     /locus_tag="OO448_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6338379.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1826..3391
                     /gene="ahpF"
                     /locus_tag="OO448_003245"
     CDS             1826..3391
                     /gene="ahpF"
                     /locus_tag="OO448_003245"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6338380.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3512..3940)
                     /gene="uspG"
                     /locus_tag="OO448_003246"
     CDS             complement(3512..3940)
                     /gene="uspG"
                     /locus_tag="OO448_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6338381.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4161..5399
                     /locus_tag="OO448_003247"
     CDS             4161..5399
                     /locus_tag="OO448_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6338382.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5630..6040)
                     /gene="rnk"
                     /locus_tag="OO448_003248"
     CDS             complement(5630..6040)
                     /gene="rnk"
                     /locus_tag="OO448_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6338383.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6270..7076)
                     /gene="rna"
                     /locus_tag="OO448_003249"
     CDS             complement(6270..7076)
                     /gene="rna"
                     /locus_tag="OO448_003249"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6338384.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7190..8653)
                     /gene="citT"
                     /locus_tag="OO448_003250"
     CDS             complement(7190..8653)
                     /gene="citT"
                     /locus_tag="OO448_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6338385.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8704..9582)
                     /gene="citG"
                     /locus_tag="OO448_003251"
     CDS             complement(8704..9582)
                     /gene="citG"
                     /locus_tag="OO448_003251"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6338386.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9557..10108)
                     /gene="citX"
                     /locus_tag="OO448_003252"
     CDS             complement(9557..10108)
                     /gene="citX"
                     /locus_tag="OO448_003252"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6338387.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10112..11644)
                     /gene="citF"
                     /locus_tag="OO448_003253"
     CDS             complement(10112..11644)
                     /gene="citF"
                     /locus_tag="OO448_003253"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6338388.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11655..12563)
                     /gene="citE"
                     /locus_tag="OO448_003254"
     CDS             complement(11655..12563)
                     /gene="citE"
                     /locus_tag="OO448_003254"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6338389.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12560..12856)
                     /gene="citD"
                     /locus_tag="OO448_003255"
     CDS             complement(12560..12856)
                     /gene="citD"
                     /locus_tag="OO448_003255"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6338390.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12871..>13768)
                     /gene="citC"
                     /locus_tag="OO448_003256"
     CDS             complement(12871..>13768)
                     /gene="citC"
                     /locus_tag="OO448_003256"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6338391.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3375 a         3491 c         3668 g         3234 t
ORIGIN      
        1 cgttttctga tcgggcaacc ccacggcctg ttgcagcgta ttttccttac tcatgtaata
       61 gatagccggc gtgacatttg cccccagatc gtccatcagt ttctcattgt cacttaacac
      121 tttcatttgc tctgtactta cgtttgcagg cacgtttagc ttaagcttgc caccagaggc
      181 ttcatattgt tgccaggttt ttgcgggatc tttggaggca agaattgccg ctgctgtcgc
      241 cgggctttct ggcttgataa ccccaaccaa caatgttctt aattgcactt tgccagaatc
      301 tacccacggg cgcgcctgct gccagaactg tttacaatat gggcagaacg gatcggcgaa
      361 gacgtagaca atgaccggcg catctttttt accgtcgagg atccagtggg attgttccat
      421 ccgttgccac atttcgcgtc cggctggtgc gtaaatttct ttttcgataa gtgtgttact
      481 caggttttca cctttctcgt tgtacatgta accagagata gcgtgcttac catcgggagt
      541 caggtagatg gtgacgccca tatcctgata ctttccgaga taacctttca ttcctccggg
      601 ggcatcgaac gttttgatga ttgtaatgcc ctgtttttca atcgctttta ctggggaagg
      661 aagttcctct gcgaaggcga ttgcaggaag cagagccagt aaaagtatct tttttaacat
      721 taatttgtcc ttttcagtca gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat
      781 aggtaagagc ttagatcagg tgattgccct ttgtttatga gggtgttgta atccatgtcg
      841 ttgttgcatt tgtaagggca acacctcagc ctgcaggcag gcgctgaaga taccaaaggg
      901 tagttcagat tacacggtca cctggaaagg gggccatttt actttttatc gccgctggcg
      961 gtgcaaagtt cacaaagttg tcttacgaag gttgtaaggt aaaacttatc gatttgataa
     1021 tggaaacgca ttagccgaat cggcaaaaat tggttacctt acatctcatc gaaaacacgg
     1081 aggaagtata gatgtccttg attaacacca aaattaaacc ttttaaaaac caggcattca
     1141 aaaacggcga attcatcgaa atcaccgaaa aagataccga aggccgctgg agcgtcttct
     1201 tcttctaccc ggctgacttt actttcgtat gcccgaccga actgggtgac gttgctgacc
     1261 actacgaaga actgcagaaa ctgggcgtag acgtatacgc agtatctacc gatactcact
     1321 tcacccacaa agcatggcac agcagctctg aaaccatcgc taaaatcaaa tatgcgatga
     1381 tcggcgaccc gactggcgcc ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc
     1441 tggctgaccg tgcgaccttc gttgttgacc cgcagggtat catccaggca atcgaagtta
     1501 ccgctgaagg cattggccgt gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt
     1561 acgtagcttc tcacccaggt gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc
     1621 tggctccgtc tctggacctg gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca
     1681 gctccggcgc gaaatacttc ggaaattcac ctaattcttc gggtgctgcg gcacccgatt
     1741 ttcttccccg caccatgatg caagctgcat ccaggtagcc gtagaggccg cttgcatgat
     1801 gatgtttaaa gcccaggaga taaacatgct cgacacaaat atgaaaactc aactcaaggc
     1861 ttaccttgag aaattgacca agcctgttga gttaattgcc acgctggatg acagcgctaa
     1921 atcggcagaa atcaaggaac tgttggctga aatcgcagaa ctgtcagaca aagtcacctt
     1981 taaagaagat aacagcttgc cggtgcgtaa gccgtctttc ctgatcacca acccaggttc
     2041 caaccagggg ccacgttttg caggctcccc gctgggccac gagttcacct cgctggtact
     2101 ggcgttgctg tggaccggtg gtcatccgtc gaaagaagcg cagtctctgc tggagcagat
     2161 tcgccatatt gacggtgatt ttgaattcga aacctattac tcgctctctt gccataactg
     2221 cccggacgtg gtgcaggcgc tgaacctgat gagcgtactg aacccgcgca tcaagcacac
     2281 tgcaattgac ggcggcacct tccagaacga aatcaccgat cgcaacgtga tgggcgttcc
     2341 ggcagtgttc gtaaacggga aagagtttgg tcagggccgc atgacgttga ctgaaatcgt
     2401 tgccaaaatt gatacgggcg cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc
     2461 ttatgacgta ttaatcgtcg gttccggccc ggcgggtgca gcggcagcaa tttactccgc
     2521 acgtaaaggc atccgtaccg gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac
     2581 cgttgatatc gaaaactaca tttctgtacc gaagactgaa gggcagaagc tggcaggcgc
     2641 actgaaagtt cacgttgatg aatacgacgt tgatgtgatc gacagccaga gcgccagcaa
     2701 actgatccca gcagcagttg aaggtggtct gcatcagatt gaaacagctt ctggcgcggt
     2761 actgaaagca cgcagcatta tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc
     2821 gggcgaagat cagtatcgca ccaaaggcgt gacctactgc ccgcactgcg acggcccgct
     2881 gtttaaaggt aaacgcgtag cggttatcgg cggcggtaac tccggcgtgg aagcggcaat
     2941 tgacctggcg ggtatcgttg agcacgtaac gctgctggaa tttgcgccag aaatgaaagc
     3001 cgaccaggtt ctgcaggaca aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc
     3061 gcaaaccacg gaagtgaaag gcgacggcag caaagtcgtt ggtctggaat atcgagatcg
     3121 tgtcagcggc gatattcaca acatcgaact ggccggtatt ttcgtgcaga ttggtctgct
     3181 gccgaacacc aactggctcg aaggcgcagt cgaacgtaac cgcatgggtg agattatcat
     3241 tgatgcgaaa tgcgaaacca acgtcaaagg cgtattcgca gcgggtgact gtacgacggt
     3301 tccgtacaag cagatcatca tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt
     3361 tgactacctg attcgcacca aaactgcata agaagaagta agattcacct gcaattgctt
     3421 agccgccggg gtcaaacctg gcggcttttt tatggcatta aaaagccctg ccgggatgtt
     3481 ccaggcaggg cggcataaat acttttatct gttagcgaat gaccagtaca ggcagattgg
     3541 cgtggcggat cacgctcgag gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc
     3601 gagaaccgat aactacaaca tcagccccca gttcttctgc caactcattg acttcatccc
     3661 gcacgctacc aaaacggaca tgttgtttaa tgcgggaagg atcgatggtg aagtggctga
     3721 ccatcgtttg cagacgttct tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat
     3781 cagcggcaaa acggtgcagg ctcaggctgg ctgacccggg tagtacgtga agtagatgaa
     3841 taactccgtc atcctgggcg aggaattcag cgtggcgaac agctttgtcg ctcaattcca
     3901 tttcaaatac atcaactggc ataatgattg tcttatacat aaccctttct ccctgttaat
     3961 catgaacaaa tcattcgcca tgattataat atttatccct gatatttgtc tggttctttt
     4021 tccttacgaa cggtttctgt gattaatata ttctcactga acaccaggaa ttctcccaaa
     4081 acctgcggta ccgcccgttt tcccgctgtg atagctaccc ttaaagactg actctttttt
     4141 gaactgtctc tggaggttgc atgaaagcat tgacttatca cggcccacat cacgttcagg
     4201 tagaaaatgt tcccgatccg ggcattgaac aggctgatga tattattctg cgtattacgg
     4261 caacggcgat ctgtggctct gacctccatc tttatcgagg caaaataccc caggttaaac
     4321 atggcgatat tttgggtcat gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa
     4381 aaaatttgca aaaaggcgac cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt
     4441 ttttctgtcg aatgcagcaa tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg
     4501 cgctcaataa aaaacagata ccagctcccg cggcattgtt tggttatagt cacctgtatg
     4561 gtggcgttcc tggtggacag gcggaatatg tccgcgtccc taaagggaat gtggggccgt
     4621 ttaaagtacc gcctttgctt tcagatgata aagccctttt cctttctgat attctgccaa
     4681 cggcatggca ggcagcaaaa aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg
     4741 gtgccggtcc agtgggattg ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga
     4801 tttttgttgt tgatcatcat ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga
     4861 tcccgattaa tttcgatgaa gatagcgatc cggcacagtc aattattgaa caaacggcag
     4921 gtcaccgggg cgtggatgca gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca
     4981 cggaaacggt gctgactaac ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt
     5041 gtattgcggc ggtcaggcgt ggcggcattg ttagcgtacc gggcgtctac gctggattta
     5101 ttcacggttt cctgtttggc gacgcctttg ataaagggtt gacgtttaaa atgggacaga
     5161 cccacgttca cgcatggctg ggtgaattat taccgttaat tgagaaagga ttactgaaac
     5221 cagaagaaat tgttacccac tatatgccgt ttgaagaggc cgcccgggga tatgagattt
     5281 tcgaaaaacg tgaagaggag tgccgtaagg tgattctggt acccggtgca caaagcgcag
     5341 aggcggcgca gaaggcggtt tcaggtctgg tgaatgcgat gccgggggga acaatatgat
     5401 cgtcaggagt ggttttcgag gtaaaggaca gccatgacga taatcgccgc cataatcaga
     5461 aatcctatca ggatgtaaaa tgcttctgcc atggttattc ccacaaacga aacggaataa
     5521 ttttgcagca aaagtgaaca gtgagaacca ggaaaaattg ctgattttgc gtaaagagga
     5581 tgcgagtgca tcctctgggc aaagcgagtt atcgcttgtg cagatgggat taaagcaggt
     5641 agtcgccagc agcttctggc tggtattcga gttccagcac ttcaaggtgg gttgcaacgc
     5701 cgcccggaag ttcccagtga atggaatcgc caacgcgcag ccccagcagt gcggcaccta
     5761 ccggagccat aacggaaagc tgagtattgc tatcggtcat ttttgccgga tacaccagcg
     5821 tgcgcacacg cacttcgcca tcgctaagat tgcggaattt aacccggctg ttcattgtca
     5881 ccacgtcgtg tggcatctct tctggcgaac acatttgggc gcgatccaac tctgcgttta
     5941 acgcgtcggc gattggcaaa ccagcatagg cgggttgctc cagcagaata tcgatgcgtt
     6001 cggcatccag gtcgttaatg atgatagttg gtctggacat ttttactcca tgtcgtcggt
     6061 actgcgagtg tcgcagataa acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg
     6121 tttaactcac atgatgatac tgactgttgc tcactctttg aagtgatttg cgtcacattc
     6181 agggaattcc tcaatgcagc gcattatgta taattcttaa tcgtcacggt ttatggaaga
     6241 cgaattagcg tgttttgtaa atcagatgat taataaccca ctttatcaat cacaaagttt
     6301 ttgccacagt tgcctgggtg tggttgtggc aagaatgagt ttgcagaaag cggagcgttg
     6361 atggcgtcag ctttgatcga gatctgaatt tcagtcaaat acgcagggtt accctggcac
     6421 gttagcttaa ctgctttcac gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag
     6481 tcacggcggc tcactgtttt accgtaatta tccgcaagga attttccggc ttcgctctct
     6541 ttaatttctt gattcaggcg taccatcgta ccgaagtatg cgtccggatc aaaaccaaag
     6601 caggcaccgt gtttggcata ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc
     6661 atgacttcac ttagtttagc ggccgtttcc agtgataacc cggtttccgg cgatgaacac
     6721 atccggctgg cgtgcgcttc tggtagattc gggattgggc gagtagcgca accgaagcgc
     6781 atccagcggc gttcatcaac accacgggca gcaaccgatt taggcaatcc tggccacaga
     6841 ccatgtacgg tcagaaaatc agctttgttg gtcgtttcgg tttgtaggcg acattcatct
     6901 cgttcgttac gatttcgatc gtgttgactc tggcaaaatc cggtttgcca ggagagggcc
     6961 aggacatagc gatcaaaatc gccatactgt tttgcctgca acgctacggc gttggcagaa
     7021 gagaagggaa gcagagaaac cgcgagcaac gcggcgttac gccagaatgc tttcataatg
     7081 ggtgtggaac tcatacatac actgaatact atctattaaa tcataaaaaa gcccgccagg
     7141 tgggcttacc tggcgggcgt gatgatttat tcagcgtttg gcgaacgtat tagttccaca
     7201 tggcgagaat cggccagcca accaacagca acatagagat gtaaatcacc ccgaagattg
     7261 cgccaagacg ccagtaatct tttgatttca catagccaca gccgtaaata atcaccccag
     7321 gaccggttgc atacggcgtc agacagccca tgataccgat agacagcacc agcaggatac
     7381 acagttgttc cattggtacg cccggaatac ctttaccgac ggccagaata accgacagca
     7441 tggttgcggt gtgcgcagac aggctggcaa acaggtagtg tgcaaagtag aacaccagaa
     7501 ccagtacaat caccgttgcg tttggtgaga atccttccag gtgcgtactc atggtaccgg
     7561 cgaaccagtc aataaagcca gaacgagtca ggccgttagc catcacaacc agagttgcca
     7621 ggttgaccag cgtgttccat gcgctgttat agcgggtaat atctttccaa ggcacaacgt
     7681 gcagggccag cattagcgaa actgccagca gaccaactgc agtagcatta atgacttcac
     7741 tgccaaaaac ccacaaacct aagctgagca atacaaggcc aatcagtgtc cactctctgc
     7801 gtgtcagcgc acccatggtt ttcagttcat cacccgccca ggttgccact tcttcactgt
     7861 gtgtgatttc cggtttgtac agcacgtagg aaagccacgg cgcaatgata agcaagataa
     7921 ccccaaccgg caggaagcag aggaaccact gcaaccagct aatctggata ccggcaattt
     7981 tgctgacgaa ctccagaccc agcacgtttg gtgccgcacc ggtgacaaac atggacgaac
     8041 tcagactggt actaatgacc atcatccaca tcaaatagcc gccaatacga cgcgcggacg
     8101 gatcgttggg gaatgattta aacagcggcg gcaggttttt aatgaccgga aaaaccgtac
     8161 ccccggtacg cgcggtgttg gacggtgtaa acggtgccag cagaatgtcg ataatgacaa
     8221 tcgcataacc caacgtcagc gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc
     8281 gacgacctaa cccggaaact tcatacccta atgcaaaaat aaatgcgcca aataccagcc
     8341 ataccgtggt gctggaaaaa ccagccagac cccatttcag cgcctgtttt tgcgcattaa
     8401 acgctgggtc agctaattct ttggcatcaa agagcaggta attactgcca ataacgcaaa
     8461 tagtaaccgc aataaaactg atcgctgttg ccggaattgg ctcgaggatc atgccgacaa
     8521 tcattgccac aaacacagcg aagtaatgcc atgcctgtgg tggcataccg tcggggacag
     8581 ggataagaaa catgacaccc atcaccacca gtggggccaa tagtttccat atattatctt
     8641 ttgctaaaga catacgggtt ctccgaaaat taatatttcc aaatttatca agtgcttaaa
     8701 taattaaatc tgtgctaaaa accaggtaag gatcagcagg tcggcactgc cgcctggact
     8761 gagatttcgt tcgatacact ccctgtcgaa ctgccggaga taatcgagat cggcgggggt
     8821 tcgaatgccc cctttttgca ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc
     8881 ctcgccaccg cgcgatgcaa cgttggtatc gccgttgatc gccatcagta ggagcaaggt
     8941 atcgagcaat gccagttcag gatctaaccc ctgatccagc agagtgaggt aatgcggcaa
     9001 ggcgtgattg atcaccagtg gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc
     9061 aagctgctgg tacaaccgtt gacctgccgt cagttgtgaa ttattggtac gcagttctcg
     9121 atcggtcagg ccacggcaga aacttgccgc cgtagaacaa acggttgttg gcgttaccgg
     9181 ttggttgagt tgaagcaaac ggccaattgc cgcacatagt agccctaaag aaaaaatgct
     9241 gcctttatgc gtgtttacgc ccgcagtggc gcggaacata tcaccttcgc aagccatacc
     9301 aattgggcgt aatccgtgga gtaccgcttc tggtgctatt tccgcactac aggcaccaaa
     9361 ttcaatgaaa cggggtagcc agccctgaat cgccagcgcg ctgcggtgga aatcttccag
     9421 cgccatatct ttatgtgcac cgcagttaat ccgatccacg aggcctggtt tcggtgacag
     9481 attgacttca gtcagcatgg cgcgccagcc cagcagggcg tactcatcga tcaatgacgt
     9541 cgcaagcttt gtggttttag ttgacgttgc aggcatcgac atcgttcagc agtgcctcca
     9601 tgcggttgag taaatcggtc agttgatggg tttttccacg cgcgcagacg gctgcgcttt
     9661 gctcgcacaa caggcagcgg cgaggcggca gtgaatagtc gcggcgggag agaatttcgc
     9721 cttcgggcgt caggacatcg atatcccata accgcccgag aggatgacta tgttcaagct
     9781 caatggtggc gagcttgagg tcgcgagccg gggcggcaat gctcaacatg ccctccggcc
     9841 cgctggcgga aaccagtgca gcctgctcct gaatttgcca gccctgtttt gcggctaagg
     9901 cacgcaaggc tgtcacgcca tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg
     9961 gcccaggcgc aaccacggta aaggagacca gtggaacagg atggcgcttg agccagacgt
    10021 gttgccgtgc ttgcctttca tcccggctga cgagcagctc gggaattgac accgcatggt
    10081 ggctggcgag ttcaggaagc aggtgcatgg cttattcctt cacctgatgc acaacatcga
    10141 tcaccgagcc atcgcggtaa cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct
    10201 gtggttcacc ggtcagcaga cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa
    10261 tgcccgcttc ctgcagacgt tctgccagtt ccggacgtgc cgggttaact gcgataccgt
    10321 ggtctgtgac cagaatatcg acactggagc ctggggtgat gcaggtcagc acgttatcca
    10381 ccagagtcgg aatacgaccg cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa
    10441 tcgcggtatc gcagtgacca ccggaagcac cacgcagtac gccgtcagag ccggtcagca
    10501 cgttaacgtt gaactgggtg tcaatttcca gcgcgctcag taccaccacg tcgagacgat
    10561 caaccgatgc gcctttcgaa ccccagttag cgtactggtt ggcgctgatt tcgatgtgat
    10621 tggggttacg ggccagcgat tgcgcagcat ggctgtcaaa gctctgcaca tccagcagtt
    10681 tgcggatcag acctttttcg tgcagatcaa ccatcgtcgc ggtaataccg ccgagggcga
    10741 agtcggcgcg aatatcgcgg ctacgcattt tgtcttccag gaaacgggtt accgccagcg
    10801 atgcgccgcc ggtgccggtt tgcatggaga aaccttcttt gaagtagcca gagttgacaa
    10861 tcacatccgc agcgctacgg gcaataagca gttcgcgcgg gttagtggtc atacgggtcg
    10921 cgccagcgcc gatttttgca gcatcgccaa cgcggtcaac tttgacgatc aaatcaacct
    10981 gatcttgctc aatgcttgcc ggattatgcg gataaggcag cagttcttcg gtaagcatca
    11041 caacctgttt tgcgttgtcg gcatcaacca ttgcatagcc gagggagccg cagcaggctt
    11101 taccggtgta accgttggca ttaccgaatt catcacagga cgggacgccg aggaaagcca
    11161 cgtcgatatt cagttcgccg ctctgtacca gatgcacacg tccgccgtga gagtggattt
    11221 gcaccggttc cgcaagcaga ccacgggaaa tttcttctgc cagtggaccg cgcaggccgg
    11281 aggtataaat gcgggtaacc acgccctggc gaatgtgttc taccagtggc gcatggcaat
    11341 cactcaggga gctggacgcc agggtcaggt ttttaaagcc catcttagcg atgacgtcca
    11401 tcaccatatt gacggtcagg tcaccgccac ggaaagcgtg atggaaggaa accgtcatgc
    11461 cgtcctgtaa accagagcga cgaatcgctt cttcctggtt ggcgcacagt ttgcgatcgc
    11521 gcgctttttc agcctggtag gtttgctttg gtgagttctg gaaagcggca agatcgcatt
    11581 cagcgcgacg attccaggcc gctacccgtt cttgtcgttg agattgttca attttctgcg
    11641 tcattttgat tgccttattc ttcgcggatg ccggaaagtt ctgcacggga gagcaccaga
    11701 cgggcacgat cgataaccgg accgtccacc atcttgccgt tcagggaaac cacgccgagg
    11761 ccttcgcgag cggcggcttc agcggcttct acgacgcggc gggcgtgatc cacttctttc
    11821 tgggtcggtg cgtagaggtt gtgcaacaga tcaatctgac gcgggttgat cagcgatttg
    11881 ccgtcaaagc ccagctgttt gatgtgggcg gcttcttgca ggaatccggc ttcgttgtta
    11941 gcgtcggaat agacggtatc gaacgcctga atacccgcag agcgcgcggc ctgcaaaatg
    12001 gaacagcgtg cgaacagcag ttcagttcct tccggggagc gttctgtacg caggttgcgc
    12061 acatagtctt ctgcaccgag ggcgataccg atcaaacgct cggaagcgtg agcgatttcc
    12121 actgcgcggg taatccccag cggagattca atcgccgcca gcaggccggt gctgccgggt
    12181 tcacgaccac aggctttttc gatacgcagg atctcttttt cgatatccag aacatcctga
    12241 gcggtatcgg ttttcggcag acgcacaacg tccgcaccac cgcgaacgac ggcttccagg
    12301 tcgttgacac cccattcgga atccagcgcg ttgacacgca caatggtttc aatatcgcga
    12361 tacagcggat gttgcagcgc gtggtaaacc atgcggcggg cggtgtcttt ttcacgcaat
    12421 gctacggagt cttcgaggtc aaacatcagg gcatcagccg ggtagatgaa ggagttgctg
    12481 accatcgcgg cattggcacc aggcacaaac aacatgctgc ggcgggtgcg agttttacgt
    12541 tgttgcagcg aagcggaaat cattggcaat cctcccatgg cagagccggg ataccgctgg
    12601 cgcgtgccag cagggcttcc agtcgtgcac gtaaaatgca gtccagtgcg cctttgtcat
    12661 cgacattcag ctgtacgccg cgcacgttgt agcgggcgag aacgtccaga atggtggtgc
    12721 gaattgcatc gccaaactgt ttctcaacgc tgctattgat ttgcaggtcg atatcctgcg
    12781 tatcgagtgg ggcgatgcgt atcatcacat ccccagactc aagggtgcct gcaacggcgg
    12841 gctggtttat tttcattttt cacctgtttc tcatgcgggg gtcttttgac gagctgccgc
    12901 gtcctggcgg gagtgctcaa gcaggttctg caaataatgc agcgtgactg cagggaccag
    12961 cggcgcgata gccgtgagat cgtttttcgc cagcagttga cgtacccggg aagcggatat
    13021 cggcatctcc tggtaacgca gccgctcaat ttcaaccagt tcgatgggcg gtgcggagat
    13081 agtcggcgtt tccagccagt agcgcatatc ctggttgtac tgggcggtaa cgcgacaaaa
    13141 gggttcagta ccgacaaagc ggtgagttac acccagcgcg ggagcgaggt actgacggaa
    13201 aatcttcaga tcaatttcgg tgtaacaatg gttaatgacg ctctgttctt taatgaagta
    13261 gcaagggaac gtagcgcggg agatgatgta ttcggagccg cgatgcacag tcaggcgtgg
    13321 aatatcggcg gtgcctttta acaccagatc caaccggtct tcatagggga agcgtgaaga
    13381 atcttctttg actaaaaaca gatgcaacca gtcgcactgt gccgcagctt gttgaatcag
    13441 ataacggtga ccattcgtga agggattggc gttcatcaca atgcagccaa tcttgttccc
    13501 tggatgacga aattttttca gcgattcggc atagcgtttc agccgcgtgg cgctgttttc
    13561 catcagcacc atcacgccgg gtacgctggt cagcgtggaa aaaccgcact ggcggaacag
    13621 cgcctcgtat tcggttttgg tataaataaa cagatgcgtg ctgtgccgct cataggcgag
    13681 gtttatcaat tcagtggcta atgtcagcgc cagtccttca ccgcgaacgg attcactgat
    13741 agcaacgcat ttaataatat ttccggca
//
LOCUS       DAJTVJ010000114        13261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000114 DAJTVJ010000000
VERSION     DAJTVJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.114"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OO448_003257"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OO448_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6338392.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO448_003258"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO448_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6338393.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO448_003259"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO448_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6338394.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAIFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OO448_003260"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OO448_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6338395.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OO448_003261"
     CDS             complement(4036..5568)
                     /locus_tag="OO448_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6338396.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OO448_003262"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OO448_003262"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6338397.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OO448_003263"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OO448_003263"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6338398.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="OO448_003264"
     CDS             complement(8663..10399)
                     /locus_tag="OO448_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6338399.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO448_003265"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO448_003265"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6338400.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="OO448_003266"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="OO448_003266"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6338401.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="OO448_003267"
     CDS             complement(12121..12855)
                     /locus_tag="OO448_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6338402.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13261
                     /locus_tag="OO448_003268"
     CDS             13056..>13261
                     /locus_tag="OO448_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6338403.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVV"
BASE COUNT         3366 a         3370 c         3488 g         3037 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 atcgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
    13261 t
//
LOCUS       DAJTVJ010000115        13254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000115 DAJTVJ010000000
VERSION     DAJTVJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.115"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(884..1975)
                     /gene="ychF"
                     /locus_tag="OO448_003269"
     CDS             complement(884..1975)
                     /gene="ychF"
                     /locus_tag="OO448_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6338404.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2092..2676)
                     /gene="pth"
                     /locus_tag="OO448_003270"
     CDS             complement(2092..2676)
                     /gene="pth"
                     /locus_tag="OO448_003270"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6338405.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2953..3231
                     /gene="ychH"
                     /locus_tag="OO448_003271"
     CDS             2953..3231
                     /gene="ychH"
                     /locus_tag="OO448_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6338406.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3286..4965)
                     /gene="dauA"
                     /locus_tag="OO448_003272"
     CDS             complement(3286..4965)
                     /gene="dauA"
                     /locus_tag="OO448_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6338407.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5089..6036)
                     /gene="prs"
                     /locus_tag="OO448_003273"
     CDS             complement(5089..6036)
                     /gene="prs"
                     /locus_tag="OO448_003273"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6338408.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6187..7038)
                     /gene="ispE"
                     /locus_tag="OO448_003274"
     CDS             complement(6187..7038)
                     /gene="ispE"
                     /locus_tag="OO448_003274"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6338409.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7038..7661)
                     /gene="lolB"
                     /locus_tag="OO448_003275"
     CDS             complement(7038..7661)
                     /gene="lolB"
                     /locus_tag="OO448_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6338410.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7875..9131
                     /gene="hemA"
                     /locus_tag="OO448_003276"
     CDS             7875..9131
                     /gene="hemA"
                     /locus_tag="OO448_003276"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6338411.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9173..10255
                     /gene="prfA"
                     /locus_tag="OO448_003277"
     CDS             9173..10255
                     /gene="prfA"
                     /locus_tag="OO448_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6338412.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10255..11088
                     /gene="prmC"
                     /locus_tag="OO448_003278"
     CDS             10255..11088
                     /gene="prmC"
                     /locus_tag="OO448_003278"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6338413.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11085..11477
                     /locus_tag="OO448_003279"
     CDS             11085..11477
                     /locus_tag="OO448_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6338414.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11481..12289
                     /locus_tag="OO448_003280"
                     /pseudo
     CDS             11481..12289
                     /locus_tag="OO448_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12325..13179
                     /gene="kdsA"
                     /locus_tag="OO448_003281"
     CDS             12325..13179
                     /gene="kdsA"
                     /locus_tag="OO448_003281"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6338415.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3343 a         3284 c         3393 g         3234 t
ORIGIN      
        1 acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc tatatagtac
       61 ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa tatcctctta
      121 tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa ttctctatta
      181 ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt acaaaggtga
      241 gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta ctataaatat
      301 ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt tgtaataatc
      361 ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat atgcgccatt
      421 tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt caatgggttt
      481 tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca ggatcgcacg
      541 taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa gtccttttct
      601 cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga tgtgtagttg
      661 aacaaaaact tacgttatat cactactaat gagatatccg tcatctgaca ctgatgtctt
      721 taattattct cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg ttacaagaat
      781 gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata tacttttgcg
      841 ctcttcatga gatttagcga aacctcatga gacaataaat taattagacg ttgaaaagga
      901 agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc attttgcctg
      961 cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg
     1021 cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg
     1081 ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag taagtttgca
     1141 ggttcagcag cttataaccg acacggatca cacggttcag gcccggttct tccagcccaa
     1201 gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag
     1261 cagcacaaac cggaaccaca acagaacctt ctttcgccgc gatttcacgc acctggtcaa
     1321 gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt gttggtttca
     1381 gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc
     1441 gcagcatacc tgcgttttcc agctggggca ggcatttttc taggaccgcc agctcagctt
     1501 tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca cgttcgcagg
     1561 tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc aatatcgtca gccgggttaa
     1621 ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg
     1681 cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct ttcgatgcgc
     1741 cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta cgttgcggtt
     1801 ttacgatttc agccagttga tccaggcgag ggtcaggcat tggtacgacg cctgtgttcg
     1861 gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc agcgcgttga
     1921 acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat cccatgattt
     1981 aaatcacctt aatatcttaa taatcaacct gttattgcta acagattgca gaaatggaaa
     2041 taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga cttattgcgc
     2101 tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt
     2161 acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa caggcggttt
     2221 gcctaacaca aaaccgacaa ctttattttt atcgccagga tgaccgattc cgatgcgtaa
     2281 acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc cattgtgacc
     2341 accatggcca ccgcccaatt taaatttggc gacgccagga ggcagatcca gttcgtcgtg
     2401 ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg caacggcttt
     2461 gccgctgaga ttcataaatg tagtcgggac taacaggcgg acatcttcgc ctccaagagt
     2521 gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc
     2581 tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt attcagcacc
     2641 ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt tttgtcctga gtgtgtacat
     2701 aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt atcaaatgca
     2761 gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag gcgtttattt
     2821 gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata acccacaggg
     2881 tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc cagaaagttc
     2941 cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct catggggttg
     3001 gggctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt accacagttt
     3061 aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg tgccattctc
     3121 tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta ctggtgggtt
     3181 cgtcactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta acaaataatg
     3241 cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata aatccgccat
     3301 cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa tgcctgcgcg
     3361 agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac atccttcggg
     3421 cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta ccggaacggc
     3481 atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt ccgtgaacaa
     3541 gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa catcgtctgg
     3601 aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca taaacagcag
     3661 cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca cggtcagcga
     3721 catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca agtcgaccac
     3781 tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca tagcggaaag
     3841 cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca gaatagagtg
     3901 gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag aacgcgcgat
     3961 ggcagctgta gcggtaatac caccaaagaa cggagcgata atattcccca gtccctgtcc
     4021 aaccagttcg ctgttcgcct tgtgtttcgt cccggtcata ccatccagta ccacggcgca
     4081 gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca gcagtgtgcg
     4141 aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca gcaccagttg
     4201 cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt agtggaattg
     4261 cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca ccgcgcaacc
     4321 agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc aaaaaacaag
     4381 aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg gcagcgccat
     4441 aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt gcagaccgag
     4501 aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga aacctaaggt
     4561 gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca tcagaatcaa
     4621 aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact gttgcgacac
     4681 gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc gtgacccacc
     4741 cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact ggggtgccac
     4801 accactacca atagccaacg ccatcgccag cgggatagca ataatcccga cggttatccc
     4861 ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc aagcgtcgat
     4921 cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa tgtttcatcc
     4981 gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca tacaaatgga
     5041 tattacagac aaaaaaaccc gccgcagcgg gtctttgagc gggttcgatt agtgttcgaa
     5101 catggcagag atcgattctt cgttgctgat acgacgaatc gcttcggcca gcatacctga
     5161 cagggtcaga gtacgcacgt tcggcagtga tttgatttca tcgctcagcg gaatggtatc
     5221 gcagacaacg acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt tgccagagaa
     5281 gatcgggtga gtcgcgtacg caaatacacg tttagcacca cgttctttca gagcttcagc
     5341 agctttacac agcgtaccgc cagtgtcgat catatcatcg accagtacgc agtcacgacc
     5401 tgcaacgtca ccgatgatat gcatcacctg ggaaacgttc gcacgcggac gacgtttgtc
     5461 gatgattgcc atatcggtat cgttcagcag cttagcgata gcgcgggcac gcacaacgcc
     5521 gccgatgtcc ggagaaacca caattgggtt atccagattc agctgcagca tgtcttccag
     5581 caggatcggg ctaccaaata cgttatcaac cggaacgtcg aagaaaccct gaatctgttc
     5641 agcgtgcaga tccactgtca gcacacggtc aacaccgacg ctggagagga agtctgcaac
     5701 cactttcgca gtgattggta cacgagcgga acggacgcga cggtcctggc gcgcatagcc
     5761 aaagtagggg ataacagcgg tgatacgacc tgcggaagca cgacgcaggg catcaaccat
     5821 aacgactaat tccatcaggt tgtcgttagt aggggcacaa gtggactgga tgatgaaaat
     5881 atcaccaccg cgtacatttt cgttgatttg tacactgact tcgccgtcgc taaagcgacc
     5941 tacagcggcg tcgccgagtg aagtgtacag gcggttggca atacgttgtg ctagttccgg
     6001 ggtggcgtta ccagcaaaaa gcttcatatc aggcacgaga agaacctcag gcatgcgtcc
     6061 attggtggaa agaatctgcc gaaaactgtg cgggccaggc atgatccttt ccaggcggtg
     6121 tattaaagag cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa actcagcttg
     6181 cccggcttaa agcatggctc tgtgcaatgg ggaaagatta acacctttcg ccacaaagcc
     6241 attgagccat tccggggctt gctctagcac ctggcgggct tcagactctg tatcaaattc
     6301 agcaaagaca caggcccctg tcccagtcag gcgcgacggg gcgtattcta acagccagga
     6361 aagcaccgca tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat cattgctgaa
     6421 ttcacatttt agcaacgttt ctattgacct ttttggcgta ttgcgcggga gttcaggatc
     6481 tttaaaaatc actggtgtcg gaatacttac accagggtgc gccaccagat accacttctc
     6541 tggcggatcc accggcgtta gtatttcacc aacgccttcg gcaaacgcgg catgcccccg
     6601 aacaaagaca ggaacatctg cgcccagcgt cagccccatt tccgccagct catccatgct
     6661 tagcccgcat tgccagagat gatttaatgc caccaggacc gtcgcggcat tggatgaacc
     6721 accgccgaga ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg caccgcttcc
     6781 cgtcggaaga cgcccgctgt ctgccgcagt tttcatcaac aaccgcgctg cgcgaacgat
     6841 caggttatct tcatgttcca cgccttcaac gggcgttaac agacgaatat ccccatcgtc
     6901 acgaagctca atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg tttgcagcgt
     6961 gtggtaacca tccgcacgct gaccggtaat gtataaaaac agattaagtt ttgccggaga
     7021 gggccactgt gtccgcatta tttcactatc cagttatcca tttttaactt gatgcgttga
     7081 ccaccgtcgg tgagttccat attggctggc atcgcaggtt gcgttttggt gtcataacca
     7141 ccataaacaa ccttccagtt tttgccattc tggctgtagg taatttcgct caggcggtac
     7201 tggtcgtcca gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca ttggcgcaag
     7261 ctgttgagcg gaattggcat tccggtcaat ttgccaatca tctcttcggc gtcatcggcg
     7321 gtataacgct gacctttatt gtcgactaac tgcacgttac ccggttgagc attcagctcc
     7381 agttccgtgc tgcccaatgg gttagtgagc agcagacggt agcgatcctg gccggtttgc
     7441 tgccagaaaa agcgggcgta cactttttgt tggtcagaaa tataagcgaa cgcgccgcga
     7501 gtctgatact gattaagatt gcgcacgtct tgctgatgct gacgccattg tggcgaatcc
     7561 gggcttttgc caggaccttt gggcgtggta acggaacagg cagtgagcac aagagcagcc
     7621 agcggtagca ggcggataag acgaaaatcg ggctggggca tagtgatgac aagtccttga
     7681 gatacgttgc agttataacc cttaatgcta gcgttaccgt ccgctatcgt ctatgttcaa
     7741 gttgtcttaa ttgccagaat ctaacggctt tcggcaatta ctccaaaagg gggcgctctc
     7801 ttttattgat cttacgcatc ctgtatgatg caagcagact aaccatatca acgttggtat
     7861 tatttcccgc agacatgacc cttttagcac tcggtatcaa ccataaaacg gcacctgtat
     7921 cgctgcgaga acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt gacagcctgc
     7981 ttgcgcagcc gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc acggaacttt
     8041 atcttagcgt tgaagagcag gataacctgc aagaggcgtt aatccgctgg ctttgcgatt
     8101 atcacaatct taatgaagaa gatctgcgta aaagcctcta ctggcatcag gataacgacg
     8161 cggttagcca tttaatgcgt gttgccagcg gcctggattc actggttctt ggggagccgc
     8221 agatcctcgg tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat atgaaggcca
     8281 gcgaactgga acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt cgcactgaaa
     8341 cagatatcgg tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg gcgcggcaga
     8401 tctttgaatc gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa accatcgagc
     8461 tggtagcgcg tcatctgcgc gaacataaag tacagaagat gattatcgcc aaccgcactc
     8521 gcgaacgtgc ccaaattctg gcagatgaag ttggcgcgga agtgattgcc ctgagtgaga
     8581 tcgacgaacg tctgcgcgaa gccgatatca tcatcagttc caccgccagc ccgttaccga
     8641 ttatcgggaa aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa ccaatgctgt
     8701 tggtggatat tgccgttccg cgcgatgttg agccggaagt tggcaaactg gcgaatgctt
     8761 atctttatag tgtggacgat ctgcaaagca tcatttcgca caacctggcg cagcgtaaag
     8821 ccgcagcggt tgaggcggaa actattgtcg ctcaggaaac cagcgaattt atggcgtggc
     8881 tgcgagcaca aagcgccagc gaaaccattc gcgagtatcg cagccaggca gagcacgttc
     8941 gcgatgagtt aaccgccaaa gcgttagcgg cccttgagca gggcggcgac gcgcaagcca
     9001 ttatgcagga tctggcatgg aaactgacta accgcttgat ccatgcgcca acgaaatcac
     9061 ttcaacaggc cgcccgtgac ggggataacg aacgcctgaa tattctgcgc gacagcctcg
     9121 ggctggagta gcagtacatc attttctttt tttacagggt gcatttacgc ctatgaagcc
     9181 ttctatcgtt gccaaactgg aagccctgca tgaacgccat gaagaagttc aggcgttgct
     9241 gggtgacgcg caaactatcg ccgaccagga acgttttcgc gcattatcac gcgaatatgc
     9301 gcagttaagt gatgtttcgc gctgttttac cgactggcaa caggttcagg aagatatcga
     9361 aaccgcacag atgatgctcg atgatcctga aatgcgtgag atggcgcagg atgaactgcg
     9421 cgaagctaaa gaaaaaagcg agcaactgga acagcaatta caggttctgt tactgccaaa
     9481 agatcctgat gacgaacgta acgccttcct cgaagtccga gccggaaccg gcggcgacga
     9541 agcggcgctg ttcgcgggcg atctgttccg tatgtacagc cgttatgccg aagcccgccg
     9601 ctggcgggta gaaatcatga gcgccagcga gggtgaacat ggtggttata aagagatcat
     9661 cgccaaaatt agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatccg gcggtcatcg
     9721 cgtgcagcgt gttcctgcta cggaatcgca gggtcgtatt catacttctg cttgtaccgt
     9781 tgcggtaatg ccagaactgc ctgacgcaga actgccggac atcaacccag cagatttacg
     9841 cattgatact ttccgctcgt caggggcggg tggtcagcac gttaacacca ccgattcggc
     9901 aattcgtatt actcacttgc cgaccgggat tgttgttgaa tgtcaggacg aacgttcaca
     9961 acataaaaac aaagctaaag cactttctgt acttggtgct cgtatccacg ctgcagaaat
    10021 ggcaaaacgg caacaggccg aagcgtctac ccgtcgtaac ctgctgggga gtggcgatcg
    10081 cagcgaccgt aaccgtactt acaacttccc gcaggggcgc gttaccgatc accgcatcaa
    10141 cctgacgctc taccgtctgg atgaagtgat ggaaggtaag ctggatatgc tgattgaacc
    10201 gattatccag gaacatcagg ccgaccaact ggcggcgttg tccgagcagg aataatggaa
    10261 tatcaatact ggttacgtga agcaataagc caacttcagg cgagcgaaag cccacgccgt
    10321 gacgctgaaa tcttattgga gtatgtcact ggcagggggc ggacttttat tctggccttt
    10381 ggtgaaacgc agctgactga cgaacaatgt cagcaacttg atgcgctact gacacgtcgt
    10441 cgcgatggtg aacccattgc tcatttaacc ggggtgcgag aattctggtc gttgccgtta
    10501 tttgtttcgc cagcgacctt aattccgcgc ccggatacgg agtgtctggt ggagcaggca
    10561 ctggcgcggt tgcctgaaca accttgccgt attctcgatc tcgggacggg taccggggcg
    10621 attgcgcttg cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt agatcgtatg
    10681 cctgatgctg tctccctggc acaacgtaat gcccagcatc tggcgatcaa aaatatccac
    10741 attctgcaaa gcgactggtt tagcgcgcta gccgggcagc agtttgcgat gattgtcagc
    10801 aatccgccgt atattgacga gcaggaccca catcttcaac aaggcgatgt ccgctttgag
    10861 ccgctcactg cgctggttgc ggcagacagt ggaatggcag acatcgtgca tatcatcgaa
    10921 cagtcgcgta acgcgctggt atccggcggc tttctgcttc tggaacatgg ctggcagcag
    10981 ggcgaagcgg tgcgacaagc atttatcctc gcggggtatc atgacgtcga aacctgccgt
    11041 gactatggtg ataacgagcg cgtaacgctc ggccgctatt atcaatgaca agtttttcta
    11101 cactgcttag tgttcatctt attagtatcg cgctttctgt tgggctatta accttacgtt
    11161 tctggctacg ttatcagaag catccacagg catttgctcg ctggacgcgc attgtgccgc
    11221 cggttgtcga tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa gcgcacatcc
    11281 tgccattttc cgggcaggca cagtggctga ctgaaaagct gtttggagtt atcatttata
    11341 tcgttttggg ttttattgca ctcgattatc gtcgtatgca cagtcagcag gcgcgcatta
    11401 ttgccttccc gctggcgttg gtggtgctgt acatcatcat taaactcgcc accacaaaag
    11461 taccgttact ggggtaagtc atgagatcgt tagctgattt cgaatttaat aaagcgccat
    11521 tgtgcgaagg catgatcctg gcttgcgaag caatccgccg cgattttccc tcgcaagatg
    11581 tttacgacga actggagcgt ctcgttagtc tggcgaagga agaaatcagc cagcttctgc
    11641 ctttagaaga gcagctggaa aaactgatcg cgctgtttta cggcgactgg ggatttaaag
    11701 cctcacgcgg cgtttatcgt ctttccgatg cattatggct ggaccaggtg ttaaagaatc
    11761 gacaggcagt gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt
    11821 gccgctgctg ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg
    11881 cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt
    11941 atggttaaag ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc
    12001 tgataacatt gaggtaatcc gcaaattgtt ggatacactc aaagcctcgt tgatggaaga
    12061 aaatcagatg gagctggcgt tacgcaccag cgaagcttta ttacaattca acccggaaga
    12121 tccctatgaa attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc
    12181 gttgaacgat ttaagttatt tcgttgaaca gtgtccggaa gacccgatca gcgaaatgat
    12241 ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat
    12301 tttactcaag attaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac
    12361 gtagcaaatg acctgccgtt cgtactgttt ggtggtatga acgtgctgga atctcgcgat
    12421 ctggcgatgc gcatttgcga gcactacgta accgtgaccc agaaactggg tatcccttac
    12481 gtgttcaaag cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg
    12541 ggcctggaag aagggatgaa aatcttccag gagttgaagc agacttttgg cgtgaaaatt
    12601 atcaccgacg ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt
    12661 cagttgccgg cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt
    12721 gcggtaatta acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt
    12781 gataaattca aagaaggcgg caacgaaaaa gtgattcttt gcgatcgcgg tgctaacttc
    12841 ggctatgaca acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt
    12901 aactcgccgg tgattttcga cgtgacccac gcactgcaat gccgcgatcc gtttggcgca
    12961 gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt
    13021 ctggcggggc tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca
    13081 tccgcgctgc cgctggctaa actggaaccg ttcctcaagc agatgaaagc gattgatgat
    13141 ctggtgaaag gtttcgaaga actggatacc agcaagtaat cttttttgct tgaaaaataa
    13201 agccttagcg ttctgcgtta agactttttt catgggtgcc agatacaaaa aagg
//
LOCUS       DAJTVJ010000116        13213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000116 DAJTVJ010000000
VERSION     DAJTVJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.116"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..807
                     /locus_tag="OO448_003282"
     CDS             <1..807
                     /locus_tag="OO448_003282"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6338416.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="OO448_003283"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="OO448_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6338417.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="OO448_003284"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="OO448_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6338418.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /locus_tag="OO448_003285"
     CDS             complement(3460..6573)
                     /locus_tag="OO448_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6338419.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="OO448_003286"
     CDS             complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="OO448_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR6338420.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="OO448_003287"
     CDS             7815..8093
                     /locus_tag="OO448_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338421.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="OO448_003288"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="OO448_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6338422.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="OO448_003289"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="OO448_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6338423.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10247..10579
                     /gene="hdeA"
                     /locus_tag="OO448_003290"
     CDS             10247..10579
                     /gene="hdeA"
                     /locus_tag="OO448_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6338424.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10695..11021
                     /gene="hdeB"
                     /locus_tag="OO448_003291"
     CDS             10695..11021
                     /gene="hdeB"
                     /locus_tag="OO448_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6338425.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11085..11732
                     /locus_tag="OO448_003292"
     CDS             11085..11732
                     /locus_tag="OO448_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR6338426.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11774..12304)
                     /locus_tag="OO448_003293"
     CDS             complement(11774..12304)
                     /locus_tag="OO448_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6338427.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12460..13026)
                     /gene="slp"
                     /locus_tag="OO448_003294"
     CDS             complement(12460..13026)
                     /gene="slp"
                     /locus_tag="OO448_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6338428.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3599 a         2982 c         3030 g         3602 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttt tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga
    10141 ttttgatatt ttatatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
    10201 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
    10261 aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca
    10321 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
    10381 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa
    10441 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
    10501 ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa
    10561 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac
    10621 aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt
    10681 gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt atgggcgctg
    10741 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
    10801 atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt
    10861 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
    10921 atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt
    10981 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
    11041 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
    11101 ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc
    11161 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
    11221 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactcgacc
    11281 ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc
    11341 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
    11401 ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
    11461 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
    11521 atttacagct gacattagtg aacggtaata tggtatcaat gctcgactgg tttaagcagc
    11581 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
    11641 ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta ttaaaaggta
    11701 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
    11761 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    11821 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    11881 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
    11941 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    12001 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
    12061 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
    12121 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    12181 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    12241 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    12301 acataaccga ctcctggtgc ggacattaac tttataatca atttgccaaa ggtaattaac
    12361 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
    12421 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    12481 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    12541 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    12601 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    12661 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactgggtc
    12721 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggttt
    12781 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    12841 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    12901 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
    12961 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
    13021 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
    13081 ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
    13141 ccttttgttt agttcaattc cattgatttt ggggcgtggc atgagtatcc atcgccccat
    13201 aatataatcc ttt
//
LOCUS       DAJTVJ010000117        12998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000117 DAJTVJ010000000
VERSION     DAJTVJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.117"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            98..1393
                     /locus_tag="OO448_003295"
     CDS             98..1393
                     /locus_tag="OO448_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6338429.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="OO448_003296"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="OO448_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6338430.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="OO448_003297"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="OO448_003297"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6338431.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="OO448_003298"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="OO448_003298"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6338432.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="OO448_003299"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="OO448_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6338433.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="OO448_003300"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="OO448_003300"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6338434.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="OO448_003301"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="OO448_003301"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6338435.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="OO448_003302"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="OO448_003302"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6338436.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAJTVJ010000118        12871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000118 DAJTVJ010000000
VERSION     DAJTVJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.118"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..695)
                     /locus_tag="OO448_003303"
     CDS             complement(<1..695)
                     /locus_tag="OO448_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6338437.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            951..1106
                     /gene="rseD"
                     /locus_tag="OO448_003304"
     CDS             951..1106
                     /gene="rseD"
                     /locus_tag="OO448_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6338438.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1103..1678
                     /gene="rpoE"
                     /locus_tag="OO448_003305"
     CDS             1103..1678
                     /gene="rpoE"
                     /locus_tag="OO448_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6338439.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1711..2361
                     /gene="rseA"
                     /locus_tag="OO448_003306"
     CDS             1711..2361
                     /gene="rseA"
                     /locus_tag="OO448_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6338440.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2361..3317
                     /gene="rseB"
                     /locus_tag="OO448_003307"
     CDS             2361..3317
                     /gene="rseB"
                     /locus_tag="OO448_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6338441.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3314..3793
                     /gene="rseC"
                     /locus_tag="OO448_003308"
     CDS             3314..3793
                     /gene="rseC"
                     /locus_tag="OO448_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6338442.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3991..5790
                     /gene="lepA"
                     /locus_tag="OO448_003309"
     CDS             3991..5790
                     /gene="lepA"
                     /locus_tag="OO448_003309"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6338443.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5806..6780
                     /gene="lepB"
                     /locus_tag="OO448_003310"
     CDS             5806..6780
                     /gene="lepB"
                     /locus_tag="OO448_003310"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6338444.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7053..7733
                     /gene="rnc"
                     /locus_tag="OO448_003311"
     CDS             7053..7733
                     /gene="rnc"
                     /locus_tag="OO448_003311"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6338445.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7730..8635
                     /gene="era"
                     /locus_tag="OO448_003312"
     CDS             7730..8635
                     /gene="era"
                     /locus_tag="OO448_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6338446.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8647..9375
                     /gene="recO"
                     /locus_tag="OO448_003313"
     CDS             8647..9375
                     /gene="recO"
                     /locus_tag="OO448_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6338447.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9387..10118
                     /gene="pdxJ"
                     /locus_tag="OO448_003314"
     CDS             9387..10118
                     /gene="pdxJ"
                     /locus_tag="OO448_003314"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6338448.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10118..10498
                     /locus_tag="OO448_003315"
     CDS             10118..10498
                     /locus_tag="OO448_003315"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6338449.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10720..10800
                     /gene="shoB"
                     /locus_tag="OO448_003316"
     CDS             10720..10800
                     /gene="shoB"
                     /locus_tag="OO448_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6338450.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10994..11254)
                     /gene="yfhL"
                     /locus_tag="OO448_003317"
     CDS             complement(10994..11254)
                     /gene="yfhL"
                     /locus_tag="OO448_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6338451.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11469..12659
                     /locus_tag="OO448_003318"
                     /pseudo
     CDS             11469..12659
                     /locus_tag="OO448_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         3278 a         3147 c         3353 g         3093 t
ORIGIN      
        1 cccgcgcgcc acgccatagc aatgccatcg ccagaagaaa tatccggatt ggtggtgtac
       61 tgatacacct tcgacgcacc gccggttgcc agtaccactg cttttgcgtg gcaggtttcc
      121 accgtttctt tatttcggtt ccatacccac gcgcctacaa cccgtcgcgt gcccggcagg
      181 ccaattttgt cagagacaat cagatcaact gcgttgctgc gctccagcac acgaatattc
      241 ggatggttct gcgccttgct caccagcgtg gattgtactt ctctaccggt ggcgtcggcg
      301 gcatgaagaa tacgacggtg actatgtcca ccttcacggg tcagatggta gctttcttcg
      361 ccattcggtt gaacgtgggt atcaaacaaa accccctggt caattaacca ttgcacacag
      421 gaacgagcat tgctggcgac aaattcaact gcatggcgat cgcaaatacc agccccggca
      481 atcaatgtgt cttccacatg cgagtcaatg ctgtcagttt catcaaacac ggcggcaata
      541 ccgccctggg cataaaatgt tgaaccttcc gttaccgggc ctttacttag aacgatgacc
      601 tgatgctggt cagccaggcg tagcgccagt gaaagtccgg ctgcgccgct accaataatc
      661 aacacgtcac atgaatgttc agggagagta ttcattttct ttgtttaatt tactaaacat
      721 tgtttggtca gcatagcatc atgttgtgcg aataaacacc tgctatttta atatttgtta
      781 cagtggctaa acacgttgac gcagggcggc gagaaaaaga gaagttactg gctggtggag
      841 gattaggtgg tgaaataaaa aggccgttgg gttactcttc aggcagttaa atgggcattt
      901 ctacacagat aatgcgatgt tcagattctg tagacttata atgatagata atgatccgtc
      961 tacagcatga caaacaaaaa cagatgcgtt acggaacttt acaaaaacga gacactctaa
     1021 ccctttgctt gctcaaattg cagctaatgg agtggcgttt cgatagcgcg tggaaatttg
     1081 gtttggggag actttacctc ggatgagcga gcagttaacg gaccaggtcc tggttgaacg
     1141 ggtccagaag ggagatcaga aagcctttaa cttactggta gtgcgctatc agcataaagt
     1201 ggcgagtctg gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg tacaagaagc
     1261 ttttattaaa gcctatcgtg cgctggattc gttccgggga gatagcgctt tttatacatg
     1321 gctgtatcgg attgctgtaa atacagcgaa aaattacctg gttgctcagg ggcgtcgtcc
     1381 accttccagt gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg gcgcgttgaa
     1441 agaaatttcg aaccctgaga acttaatgtt gtcagaagaa ctgagacaga tagttttccg
     1501 aactattgag tccctcccgg aagatttgcg catggcaata accttgcggg agctggatgg
     1561 cctgagctat gaagagatag ccgctatcat ggattgtccg gtaggtacgg tgcgttcacg
     1621 tatcttccga gcgagggaag ctattgataa caaagttcaa ccgcttatca ggcgttgacg
     1681 atagcgggat actggataag ggtattaggc atgcagaaag aacaactttc cgctttaatg
     1741 gatggcgaaa cgctggatag tgagctgctt aacgaactgg ctcataaccc agaaatgcag
     1801 aaaacctggg aaagctatca cttaatccgt gattcaatgc ggggtgatac tcccgaggtg
     1861 ctccatttcg atatctcttc acgcgtgatg gccgccattg aagaagagcc agtacgtcaa
     1921 ccggcgacat tgatcccgga agcccagcct gcgccgcatc aatggcacaa aatgccattc
     1981 tggcagaaag tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc agcatgcgta
     2041 tcgcttgcag ttatcgttgg cgtccagcac tataatggac aatctgaaac gtcccagcag
     2101 cccgaaacgc cggtatttaa tacactgccg atgatgggta aagccagccc ggtaagcctg
     2161 ggagtacctt ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca ggagcagcgt
     2221 cgtcgcatta atgcaatgtt gcaggattac gaactgcaac gccgactcca ctctgaacag
     2281 cttcagtttg agcaggcgca aacccagcaa gccgctgtac aggtgccagg acttcaaact
     2341 ttaggaacgc aatcgcagta atgaagcaac tttggtttgc catgtcatta gtgacaggta
     2401 gcctgttatt ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta ttacagcaga
     2461 tgaacctggc cagtcagtca ctgaattacg agctgtcatt catcagcatc aataaacagg
     2521 gtgttgagtc tctgcgttat cgacatgcac gcctcgataa ccgtcctctt gcacaattgt
     2581 tgcaaatgga tggcccgcgc cgggaagtgg tacagcgcgg caatgaaatc agctattttg
     2641 aaccgggact tgaaccgttc acgcttaatg gcgattacat tgttgattct ctgccatcgc
     2701 ttatctatac cgatttcaaa cgcctttctc cttactacga ctttatctcc gtcgggcgca
     2761 cgcgtattgc tgatcgtctt tgcgaagtca ttcgcgtggt tgcccgagat ggtacacgct
     2821 acagctacat cgtgtggatg gacactgaat cgaaattacc gatgagggtt gatctccttg
     2881 atcgcgatgg tgaaacgctg gaacaatttc gcgtgatttc ttttaacgtc aatcaggata
     2941 tcagcagcag tatgcagacg ctggcgaagg caaatttgcc gccgttgctt tctgttcctg
     3001 taggtgaaaa agctaaattc agctggacgc caacctggtt gccacagggt tttagcgaag
     3061 tttccagtag tcgacgtccg ctaccgacga tggacaacat gcctatcgaa tcacgtctct
     3121 attccgacgg attattcagc ttctcggtaa acgttaaccg cgctacgcca tcgagcaccg
     3181 atcagatgtt gcgcaccgga cgcagaaccg tcagtacaag cgtacgtgat aacgccgaaa
     3241 tcaccattgt cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag aatattaagt
     3301 tcggggcagc gcaatgatca aagagtgggc taccgtcgtc tcctggcaaa acgggcaggc
     3361 gctggtcagt tgtgatgtta aagcctcatg cagcagctgc gcttcacgcg ccggttgcgg
     3421 tagccgcgtg ttgaataaac ttggcccgca aaccacgcat accattgtcg taccctgtga
     3481 tgaaccgtta gtgccggggc aaaaagtgga attggggatc gccgaaggca gcctgcttag
     3541 ctccgcatta ctggtttata tgtcgccgct ggtgggatta ttccttatcg cttcgctatt
     3601 tcagctactc tttgcttctg acgttgcagc attatgtggt gcgatccttg gcggtattgg
     3661 cgggtttctg attgcgcgcg gttactcgcg taagtttgct gcccgggcag aatggcaacc
     3721 gatcatatta agcgtggcat tgccgcctga ccttgtgcga tttgaaacat cttcggaaga
     3781 cgcgagccag tgatttcact ggctttgttg cattttgcat aacaaatgcg aggacgtttc
     3841 cagataccac aatccatacg gtatgaacat ttcctcgcct caatgttgta gtgtagaatg
     3901 cggcgtttct attaatacag acgttaagct cagaacagcg acttctaaaa gcctggttaa
     3961 ccgggcatta aggcacaata atcatacttt atgaagaata tacgtaactt ttcgatcata
     4021 gctcacattg accacggtaa atcgacgctg tctgaccgta ttatccagat ctgcggtggc
     4081 ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga gcgtgagcgt
     4141 ggcattacca tcaaagcgca aagcgtgacg ctggactaca aagcgtctga cggcgaaacc
     4201 tatcagctta actttatcga caccccgggc cacgtagact tctcctatga agtttcccgt
     4261 tcgctggctg cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg cgtggaagcg
     4321 caaaccctgg caaactgcta caccgccatg gaaatggatc tcgaagtggt gccagtactg
     4381 aacaagattg acctgccggc agccgatcct gaacgcgtgg cggaagaaat tgaagatatc
     4441 gtcggcatcg acgccactga cgcggtgcgc tgttcagcga aaaccggcgt tggcgtgcag
     4501 gacgttctcg aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc ggaaggctcg
     4561 ttgcaggcac taattatcga ctcatggttc gacaactacc tgggcgttgt ttcacttatc
     4621 cgtattaaaa acggcaccct gcgtaagggc gacaaagtga aagtcatgag taccgggcag
     4681 acctataacg ccgaccgtct gggcatcttc acgccgaaac aggttgaccg cactgaactg
     4741 aaatgtggcg aagtaggctg gctggtatgt gcgattaaag atatccatgg cgctccagtc
     4801 ggcgatacct taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg ctttaagaaa
     4861 gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt ccgacgacta tgaagccttc
     4921 cgtgacgcgc tgggtaaact cagcctgaat gatgcctcac tgttctatga gccggaaagc
     4981 tccagcgcgc tgggctttgg tttccgctgc ggcttccttg gcctgctgca catggagatc
     5041 atccaggaac gtctggaacg tgaatacgat ctggatctga tcaccactgc gccgaccgta
     5101 gtgtatgaag ttgaaaccac gtcaagggaa gttatctacg tcgacagccc atccaagctg
     5161 cctgcggtaa ataacattta cgaactgcgc gagccgattg cagagtgtca catgctgctg
     5221 ccgcaggcat atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg cgtgcagacc
     5281 aatatggttt accacggtaa tcaggtggcg ctgacgtacg agatcccgat ggcggaagtg
     5341 gtgctcgact tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc tctggattac
     5401 aacttcaaac gcttccaggc gtccgacatg gtacgtgtag acgtattaat caacggtgaa
     5461 cgtgttgatg cgttggcgct gatcacccac cgtgataatt cgcaaaaccg cggtcgcgag
     5521 ttggtggaga agatgaaaga tctgatccca cgccagcagt ttgatatcgc cattcaggca
     5581 gcgattggta cgcacattat tgcgcgatcc accgtgaaac agctgcgtaa aaacgtactg
     5641 gctaaatgtt atggcggcga tatcagccgt aagaaaaagc tgctgcagaa gcagaaagaa
     5701 ggtaagaaac gcatgaagca gatcggtaac gtcgagctgc cgcaggaagc gttcctcgcc
     5761 attctgcacg tcggcaaaga caacaaataa cccttaggag ttggcatggc gaatatgttt
     5821 gccctgattc tggtgattgc cacactggtg acgggcattt tatggtgcgt ggataaattc
     5881 tttttcgcac ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc cggtgactca
     5941 ctggataaag caacgttgaa aaaggttgca ccgaagcttg gctggctgga aaccggagct
     6001 tctgtttttc cggtgctggc tatcgtattg attgtacgtt cgtttattta tgaaccgttc
     6061 cagatcccgt caggttcgat gatgccgact ctgttaatcg gtgattttat tctggtagag
     6121 aagtttgctt atggcattaa agatcctatc taccagaaaa cgctgatcga aaccggtcat
     6181 ccgaaacgcg gcgatatcgt ggtctttaaa tatccggaag atccaaagct tgattacatc
     6241 aagcgcgcgg tgggtttacc gggcgataaa gtcacttacg atccggtctc aaaagagctg
     6301 acgattcaac cgggatgcag ttccggccag gcgtgtgaaa acgcgctgcc ggtcacctac
     6361 tcaaacgtgg aaccgagcga tttcgttcag accttctcac gccgtaatgg tggggaagcg
     6421 accagcggat tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat tcgtctttcc
     6481 gagcgtaaag agacactggg tgatgtgacg caccgaattc tgacagtgcc gattgcgcag
     6541 gaccaggtgg ggatgtatta ccagcagcca gggcaacaac tggcaacctg gattgttccg
     6601 ccgggacaat acttcatgat gggcgacaac cgcgacaaca gcgcggacag ccgttactgg
     6661 ggctttgtgc ctgaagcgaa tctggtcggt cgggcaacgg ctatctggat gagtttcgat
     6721 aagcaagaag gcgaatggcc gacaggtgtg cgcttaagtc gcattggcgg catccattaa
     6781 tagcccatct tcgttcacgt tgtcgccgtt atggcgacaa cgtgaattat ttatgagata
     6841 aatctcccgt ggctaacgac atcccccgtc gttgtgtata gaatattccc ccgaagttta
     6901 aggttggcac ctccaggttg ccacggcaca cgaaacagcg ttggtcccca tataccggta
     6961 aactgaaact gcagcgaagc agttagcaga gccatgtata tcaggtctgt ttcgtgtgct
     7021 gaattgttga cgcatttatt tattggtatc gcatgaaccc catcgtaatt aatcggcttc
     7081 aacggaagct gggctacact tttaatcatc aggaactgtt gcagcaggca ttaactcatc
     7141 gtagtgccag cagcaaacat aacgagcgtt tagaattttt aggcgactct attctgagct
     7201 acgttatcgc caatgcgctt tatcaccgtt tccctcgtgt ggatgaaggc gatatgagcc
     7261 ggatgcgcgc cacgctggtc cgtggcaata cgctggcgga actggcgcgc gaatttgagt
     7321 taggcgagtg cttacgttta gggccaggtg aacttaaaag cggtggattt cgtcgtgagt
     7381 caattctcgc cgataccgtc gaagcattaa ttggtggcgt attcctcgac agtgatattc
     7441 aaaccgtcga gaaattaatc ctcaactggt atcaaactcg tttggacgaa attagcccag
     7501 gcgataaaca aaaagatccg aaaacgcgct tgcaagaata tttgcagggt cgccatctgc
     7561 cgctgccgac ttatctggta gtccaggtac gtggcgaagc gcacgatcag gaatttacta
     7621 tccactgcca ggtcagcggc ctgagtgaac cggtggttgg cacaggttca agccgtcgta
     7681 aggctgagca ggctgccgcc gaacaggcgt tgaaaaaact ggagctggaa tgagcatcga
     7741 taaaagttac tgcggattta ttgccatcgt cggacgtccg aacgttggca aatccacatt
     7801 gttgaacaaa ctgctggggc agaagatctc catcacctcc cgcaaagcgc aaacaactcg
     7861 tcaccgcatt gtggggatcc atactgaagg cgcgtatcag gcgatctacg ttgatacccc
     7921 tggcctgcat atggaagaaa aacgcgctat taaccgcctg atgaacaaag cggcgagcag
     7981 ctctattggc gacgttgagc tggtgatttt tgtcgttgaa ggcacccgct ggacgccgga
     8041 cgacgaaatg gtgctcaaca aactgcgcga tggcaaagcg ccggtgatcc tcgcggtgaa
     8101 caaagtggat aacgtgcagg aaaaagccga tctgctgccg cacttacagt tcctggcaag
     8161 tcagatgaac ttcctcgata tcgtgccgat ctctgctgaa accgggctga atgtcgacac
     8221 tatcgcagca atcgtgcgta agcatctgcc tgaagcgacc catcacttcc cggaagatta
     8281 catcaccgat cgctcacagc gttttatggc gtctgaaatc atccgcgaaa aactgatgcg
     8341 tttcctcggc gctgaactgc cgtactccgt gaccgtggag atcgaacgtt tcgtctctaa
     8401 cgaacgcggt ggttatgaca tcaatggttt gattctcgtt gagcgtgaag ggcagaagaa
     8461 gatggttatt ggcaacaaag gggccaagat caaaaccatc gggattgaag cgcgtaaaga
     8521 catgcaggaa atgttcgaag cgcctgtcca ccttgaactg tgggtaaaag tgaaatccgg
     8581 ttgggccgac gacgaacgcg cactgcgcag tctcggttac gttgacgatc tttaagagtc
     8641 actccgatgg aaggctggca gcgcgcattt gtcctgcata gtcgcccgtg gagcgaaacc
     8701 agcctgatgc tggacgtctt cacggaggaa tcgggtcgcg tgcgtctggt tgccaaaggc
     8761 gcacgctcta aacgctctac tctgaaaggt gcattacagc ctttcacccc tctcttgcta
     8821 cgtttcggcg ggcgtggcga agtcaaaacg ctgcgcagtg ctgaagctgt ctcgctggcg
     8881 ctgccattaa gcggtatcac gctttacagc ggtctgtaca tcaacgaact tctctcccgc
     8941 gtactggaat acgagacgcg cttctctgaa ctctttttcg attacttgca ctgcattcag
     9001 tctcttgcag gtgccactgg tacgccagaa cccgcgctgc gccgctttga actggcactg
     9061 ctcgggcatc tgggctatgg cgtcaatttt acccattgcg cgggtagcgg cgagccggta
     9121 gatgacacca tgacgtatcg ttatcgcgaa gaaaaagggt ttatcgcaag cgtcgttatc
     9181 gacaataaaa cgttcaccgg aaggcagtta aaagcgttaa acgcgcggga atttcctgac
     9241 gcagacacac tacgcgccgc gaaacgcttt acccgcatgg cgcttaagcc gtatcttggc
     9301 ggtaaaccat taaagagcag ggaactgttc cggcagttta tgcctaagcg aacggtgaaa
     9361 acacattatg aatgatgagg attgtcatgg ctgaattact gttaggcgtc aacattgacc
     9421 atattgcaac gctgcgtaac gcacgcggta ctgcttaccc ggatccggtg caggcggcgt
     9481 ttattgccga gcaggcggga gcggacggca ttaccgtgca tttacgtgaa gatcgccgcc
     9541 acattactga ccgcgatgtg cgcatcctgc ggcagacgct ggatacccga atgaatctgg
     9601 agatggcagt gaccgaagag atgctggcga tcgccgttga gacgaagcca catttttgct
     9661 gcctggtacc ggaaaagcgt caggaagtaa caaccgaagg cggcctggac gttgcagggc
     9721 agcgtgaaaa aatgcgcgat gcctgcaaac gtctgacaga tgccgggatt cagatttctc
     9781 tctttatcga cgccgatgaa gagcagatca aagctgcggc agaggttggc gcaccgttta
     9841 tcgagatcca caccggttgc tatgctgatg ccaaaactga cgccgaacag gcgcaagagc
     9901 tggtgcgtat cgccaaagcc gcgacctttg ccgctagtct cggtctgaaa gttaacgccg
     9961 gacacggtct gacctatcac aacgtgaaag ccattgccgc catccctgag atgcatgaac
    10021 tgaatatcgg tcatgccatt attggccgtg cagtgatgac cggactgaaa gatgcggtgg
    10081 cagaaatgaa gcgtctgatg ctggaagcgc gtggctaatg gcaatattag gtttaggcac
    10141 ggatatcgta gagatcgctc gcatagaagc agtgatcgcc cgatccggtg agcgcctggc
    10201 acgccgcgta ttaagcgata acgaatgggc aatctggaaa acgcaccacc agccggtgcg
    10261 ttttctggcg aagcgttttg ctgtgaaaga agccgcagca aaagcgtttg gcaccgggat
    10321 ccgcaatggt ctggcgttta atcaatttga agtattcaat gatgagctcg gcaaaccacg
    10381 gctacggcta tggggtgagg cattaaaact ggcggaaaag ctcggcgttg taaatatgca
    10441 tgtaacgctg gcagatgaac gccactatgc ttgtgccacg gtaattattg aaagttaaaa
    10501 taataccgtt ctctttaaaa agtaaaaccc ccgaactggt aaggaacggg ggttttactt
    10561 tttaacttta attttgtgca gcatgcaccc tcaacaacag cgattatgga tcagtgtact
    10621 tatcttttac aagcaacttg caatctttag cataaaaact cgagccttta cgaagaaagc
    10681 aatactgaac ggctgcattc cttgtttaag gaaaaacgaa tgactgattg ccgatacctg
    10741 attaaacggg tcatcaaaat catcattgct gttttacagc tgatccttct gttcttataa
    10801 cacaaggaaa cgtacttaag gtgcgtccgg tgaaccaatc ggacgcgcct ttaataacta
    10861 taaataagtg tcttggcaga tactatataa attaacttag tgaatgatta tgctaatgtc
    10921 atcaattaaa taaatataat ggcgttaagg cttcccagta atataattaa tactctactt
    10981 ccagagtaga atattaaatt ttatccgcgt ggtgcatcag cacaaattta tcccacaact
    11041 gttcttctgt ctcgacatgc gccggatctt tcacaatagt attggggatc gggcacacct
    11101 tctggcaggt tggtgtctcg tagtgcccta cgcattcggt acacttatcg ctgttaatct
    11161 cgtagatatg atctcccatt gaaatcgcct cattcgggca ttcgggttca cacatatcac
    11221 aattaataca gcgtttagta attagtaaag acatttcaat ggattaccgt taaatcattt
    11281 taaaatcagt aagttgtatc gagtttgtat gctttactgt cattaactta ctgtatgttg
    11341 atccagtgta tttaaccttg ataaactcag tccagcaaca caaaaccgca acacattgca
    11401 ttttgtcccg tagaaaagac ttgtatgtgt gagcttgttt tctgcgccta cgcagataag
    11461 gattgagaat gccgcgcact gtaacacata atccggatag ccccaataat gacgatgttt
    11521 tagccgcatc tgaaaaatgg gacgcctgta aaccccccta taccagcgca cacatgaaaa
    11581 tctgtgttgc tgccgccaaa atcatcctcg ctgcttccgg cgtggctcgc cgttccaaat
    11641 acgaaaaaga gaactatctc cgtatcgatt tcagcaaagc cggtaaggtt acattttacg
    11701 ccgagtttcc aaaaaagatg ggcctcaaag gtaaaaagct cggcgagtgg ccggagctcg
    11761 ctatccagct ggcgcgcgaa aaagcactag gtatggctga cggtggactg cgggcagagt
    11821 ccgtacatgc agcgctggaa atgtaccggg atgacctcaa agccaaagta gcccggcaga
    11881 agctgagccc ggacagtttc acaacctacg gggtgcgtat cgaccggatt aaagcaacgt
    11941 tcggcgagcg tgaggtgttc agcgacgtaa catacaatcg gctggtggaa gtgctggacg
    12001 agtggatcgc cactcgctcg aacaataacg ccctggagtt gtttgccgag ctccgtcggt
    12061 tctggaagtt ctgcgcacct actctttgca acggccgcaa tgttgccgcc agtctgccag
    12121 atgattatgt ttcctcccgc gtacagaaac ctacccccac acggcttttt accgatattg
    12181 aatcaatcgc ccgactctgg ctcaatgttg ctgcctgcac ctctgtacac cagaagaatg
    12241 ctgttcgctt catgatcatc actggtgttc gtccgattaa tgtccataac ctgcgctggg
    12301 actacgttta cgaggaggct ggtgaaattg tttatccgga aggggttatc ggcatgcgag
    12361 gggctatgaa aacacaaaag gctttccgcc tgccgataac gcctgagatc cggcggatta
    12421 tcgacgagca gaaagcctgg cgtgattcag ttcctgagtg caacagggat tatgtatttt
    12481 tgcagccacg tgatccaatg cagccatttt caaaacgatc actggataag ctggtgaaaa
    12541 catacagccc ggacggggct gtaaaaggaa taaaacatga tgggactgtt aaagggaaag
    12601 acggtgcatt taatacgatg tgccgtaaat tccttaagag caatgttatt gccttgatcg
    12661 ataggaatta aaaccccaaa aagattaaaa aaacaccaca aaacggatgt ttcttcaaca
    12721 ccacttttgc tcatatgaac ggaaccgacg attaaactgg atggctctga ttgattcagg
    12781 gtatgaatgg cggttttttg ctccgtttcc ctcaaaatgg acgcaacttt ccctctgcgg
    12841 ctctcagccg caccaccgca tccgggccag c
//
LOCUS       DAJTVJ010000119        12670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000119 DAJTVJ010000000
VERSION     DAJTVJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.119"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..156
                     /locus_tag="OO448_003319"
     CDS             <1..156
                     /locus_tag="OO448_003319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338452.1"
                     /translation="DRSGRAAPVAPETALPAHLTVCKATAPDGAVNPSAATPAACFPL
                     WTIRGLE"
     gene            400..834
                     /locus_tag="OO448_003320"
     CDS             400..834
                     /locus_tag="OO448_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HCR6338453.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            926..1192
                     /locus_tag="OO448_003321"
     CDS             926..1192
                     /locus_tag="OO448_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338454.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            1257..2198
                     /locus_tag="OO448_003322"
     CDS             1257..2198
                     /locus_tag="OO448_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6338455.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRQRDMLMCLDNLVRALQDIQDEEQITVLFNYL
                     LNGSEHVTVEFLQTLAQRLPQHEDSIMTLAERLKQEGIQQGIQQGIQQGIQQGVQQGA
                     LQKAREIARELRNAGMPAAQICQLTGLSEAELKNITH"
     gene            2260..2430
                     /locus_tag="OO448_003323"
     CDS             2260..2430
                     /locus_tag="OO448_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338456.1"
                     /translation="MTIAERLIQKGFEKGALEVAREIACRLRDMGWTPERIQEATGLS
                     GEELKKLFPDEQ"
     gene            complement(2461..2712)
                     /locus_tag="OO448_003324"
     CDS             complement(2461..2712)
                     /locus_tag="OO448_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6338457.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(2746..2943)
                     /locus_tag="OO448_003325"
     CDS             complement(2746..2943)
                     /locus_tag="OO448_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338458.1"
                     /translation="MQGAPHPARLKKNDGATMACGGHWHTTPAQVKTEKTARHLQSKK
                     VKFLHFFTLIFRADLVVMVIK"
     gene            3292..4140
                     /locus_tag="OO448_003326"
     CDS             3292..4140
                     /locus_tag="OO448_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6338459.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFIGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     SLPPRV"
     gene            complement(4225..4560)
                     /locus_tag="OO448_003327"
     CDS             complement(4225..4560)
                     /locus_tag="OO448_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCR6338460.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTASKKTDSAR
                     KDGIDMNNN"
     gene            4794..5126
                     /gene="nikA"
                     /locus_tag="OO448_003328"
     CDS             4794..5126
                     /gene="nikA"
                     /locus_tag="OO448_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HCR6338461.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            5137..7836
                     /gene="nikB"
                     /locus_tag="OO448_003329"
     CDS             5137..7836
                     /gene="nikB"
                     /locus_tag="OO448_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HCR6338462.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(7873..10164)
                     /locus_tag="OO448_003330"
     CDS             complement(7873..10164)
                     /locus_tag="OO448_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HCR6338463.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERACINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(10157..11227)
                     /gene="trbB"
                     /locus_tag="OO448_003331"
     CDS             complement(10157..11227)
                     /gene="trbB"
                     /locus_tag="OO448_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HCR6338464.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPPEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            complement(11246..12454)
                     /gene="trbA"
                     /locus_tag="OO448_003332"
     CDS             complement(11246..12454)
                     /gene="trbA"
                     /locus_tag="OO448_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HCR6338465.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            12587..>12670
                     /locus_tag="OO448_003333"
     CDS             12587..>12670
                     /locus_tag="OO448_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6338466.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELG"
BASE COUNT         3060 a         3178 c         3380 g         3052 t
ORIGIN      
        1 gaccgttccg gtcgcgccgc ccctgtcgcg ccggaaacgg cgctccctgc acaccttacg
       61 gtgtgtaagg caactgcgcc tgatggcgca gtcaaccctt ccgccgccac ccctgcggcc
      121 tgtttcccgc tgtggactat ccgggggctg gagtaaccac atcccgtttc agcagtccag
      181 gggaagatat accgtccaca aaatttttag ccataaaccc ggcaaaaaat tttgcgtccg
      241 cccccggaca gctgccccgt gacgtggtga cggacgcccc atcggaaagg tccactgcgg
      301 ggagaaaccc cgaagaagtg gacggcagaa ggggctttcg cccctccggg gagcaaccgg
      361 tcggcccggc gacctgattc agcagaagga gatgttttca tgacagtcag cagcacgatt
      421 tccgtttttt gccgcgacgg ggtgttccgt accgtttact gccacctgca cggggagccg
      481 acctggaacg gtcgcatcct gcatactcac tatgccaccg gtcagcaggc cgaagccctg
      541 gttgaacacg gtgatatccg ttgcctcggt ccccgttgtg acaaacccgc cgggcatacg
      601 cttcagaacc cggtggacgg tgtgacggct tattacggac gtgacagtgg tttccggatg
      661 gacagtgagg cgcgtgagta ccgttctttc agggaggcta ttgccactga aagcactgaa
      721 gaggtgcgct tccattatgt gttcatcgac ggctactgga aggtgatgta ccgcacgccg
      781 gaaggctgga agatgaaagc gctcgcgctg gcactgcgtc gctgtccgaa atgaaaaaaa
      841 ggcagggcga taaaccctgc ctctctccgc cgttacttcc ccgccaggaa gagcggcagt
      901 catcaacaac ataaggagta atattgtgag accatcaatt atcttcgcaa ccgccgagta
      961 tgtaaagcgt ctgcgtgaag agtgcctgcg ggagaataaa cccctgcacc gccatacccg
     1021 cttcagacgt caggagctgg cacaggatga gattaacccg gacgtcctgg cgatgagcgg
     1081 ccatatcgcc agacgctgca gtgagcagaa gcgggtgcgt atccccgcta tgaaagtcag
     1141 cgaatggggc cacctgctcc gcgcgcttga aattgagcgg gtctgccact gattaaccct
     1201 tgccgtctgc ctgtgcaggc ggcaggatac gttcagacat ctcaccagga ggaaccgtga
     1261 gtaagaagaa aaacaccaca acgcccacgc cgcatgatgc cgcgttccgg tcgttcctgg
     1321 cgaatcccga cgtcgccaga gattttctgg aactgcatct tcctgcggag taccggcagt
     1381 tgtgcgacct gtccacgctg aagctggaac ccgccacctt tgttgagccg gacctgcatc
     1441 agtacgccag cgatatcctc tggagcgtga aaaccaccgg gggtgaagat ggttatgttt
     1501 atacgctcat cgagcaccag agcaccgaaa atctgtacat gccttttcgc atgttacgct
     1561 acagtgtggc ggcgatgcag agacatctgg agcagcacaa aacgttgcca ctggtcattc
     1621 cggtgctgtt ctatcacggt gagcgcagcc cgtacccgta cagcatgaac tggctggact
     1681 gttttgagaa tccggcgctt gcggctaaaa tatacacaaa gccgtttccg ctggttgata
     1741 tcactgtcgt tgatgacaat gaaatcatga accatcgccg gatggccgca ctgacgctgc
     1801 tgatgaagca tatccgtcag cgggatatgc tgatgtgtct ggacaacctg gtcagagcac
     1861 tgcaggacat ccaggatgaa gagcagatta cagttctttt caactatctg cttaatggca
     1921 gcgaacatgt aacggttgaa tttttgcaga cgctggcgca gcgactgccg cagcacgagg
     1981 acagcattat gacactggca gaacgtctta agcaggaagg tatccagcaa ggtatccagc
     2041 agggtatcca gcagggtatc cagcagggag ttcagcaggg ggcgcttcaa aaagcacggg
     2101 aaatcgcccg ggagctccgg aacgcgggaa tgccggcagc acaaatctgt cagctgaccg
     2161 gactttctga agctgaactg aagaacatca ctcactgatg agagccaaaa ggcccggcaa
     2221 aatgccgggc cttttgccag caggagcaga gaaaacagta tgacgattgc agaacgtctt
     2281 atacagaaag gttttgaaaa aggtgctctt gaagtggcgc gggaaatagc ctgccggctt
     2341 cgggatatgg gctggacgcc ggaacggatt caggaggcca cgggactttc cggtgaagaa
     2401 ctgaaaaaac tgtttcctga tgagcagtag cctggcattc tgccaggctg tgaaccacgc
     2461 tcacacatcc tctgccgttt cccccagttc attttccaga cgcctgatga tatctgcctt
     2521 gcgcatcccc tgcatgtgag gcagcaggtc acggactgaa agtgcctggg tggtcctcca
     2581 gacggtgaca tccacctgtt ccgcattttc ccaggcgtgc catgttctct gtgtcacgcc
     2641 aaattctctg gccgcttttt cgcgtgacca gagcatgctt tttcgccaga ttcgcagttc
     2701 ccagccggtc ataaaacacc tttgaaaaag tgaaaaaact tcactttatt ttatcaccat
     2761 aaccaccaaa tcagcgcgaa aaataagtgt gaaaaagtga aggaatttca ctttttttga
     2821 ttgtaggtgt cgtgctgttt tctctgtttt cacctgtgcg ggagtggtgt gccagtgacc
     2881 accacatgcc attgttgcgc cgtcgttttt cttcagtcgc gccggatgcg gcgctccctg
     2941 cacaccttac ggtgtgtaag gccactgcgc ctgatggcgc agctgtctct gcctgtctgc
     3001 cgccgcactg cggcctgttc tccgcagatg gactttccgg gggtacatac acgaccacat
     3061 cccgtttcag tcgtccaggg gagagatgta ccagccacaa aactttttgc cgcaggcgtc
     3121 aaaaactttt gcgtctgccc ccggacaact gccccgtgac gtggtgagcg tcccccgtcg
     3181 gaaaggtcca ctgcggggaa atccccgaag acccggacgg cagaaggagc ttcgctcctc
     3241 cgggaaatga cctggctgac ccggcagtcg tgacgatgag gagacaatgt gatgaatcag
     3301 actttaccca ctgctgacct gaatactgcc ggcacgacag atgttattcc gtctgtggcc
     3361 atcgaccgca tcatcgcgca gcgtaacgaa ggtattgcac tgttcatgca ggcgatggaa
     3421 tgcctggcga cagcgcgcag gattctgctt gatgcgtcag gtgatatttt tctttacggt
     3481 tttgaagact gcgtgaccga ctccgttcgg tgcatggata aaccggaaga agcgaaaagg
     3541 aatatcaccc gtcttgccga ccggaaaatc tgggaccgcc tgatgacgga tacggggatg
     3601 tacaccttca tgagttcatg tcagcgtgat gagtggaaca gccagctgat gagcgacacc
     3661 tgtcctgaaa tcaccctgga caatgtactg gcaacttttc gccatctgaa tgccagtaag
     3721 atgcagacat ttgaacaggg actgattgat gtctaccgga aattgtcatg ggattacaga
     3781 accaataatc cctgccgtct gggcaagaga atcattattg aaaacctgct gtaccgctgg
     3841 agtaacgggc gtgttacgct ggactgcagc ggacgggagg cactggatga cctggtacgt
     3901 ccgttttatc tgctggaggg gcgcaacgtt cctgacttcc ggagcagcat cggggcgcag
     3961 tacggtgaat ttatcgggaa cggcgacaat gtcggtaagc tgttagaagg ggaatatttt
     4021 acggtgcgtg gctaccagaa agggaccgta cacattgtct ttaagcgtcc tgaccttgtt
     4081 gaaaaactga atgatattat tgcacggcat tatcctggtt cattgccgcc acgagtctga
     4141 ttaaacagaa aagccctggt atttatgccg gggctttttt tctgtaatca tcataatatc
     4201 agaacatgat gtgacattgt cacatcaatt gttgttcata tcgatgccat ctttcctggc
     4261 tgaatctgtc tttttagatg ctgttttaga ggaagaacgg cgggatggct ttttcgattc
     4321 tgcttcccga ataatgtcac gaagtgcctt ctctcctact tcataccctg catttttgag
     4381 cgtgtcacga acatctgcca gtgtataacc ctttgtcctt accagaacaa taatgtcatc
     4441 acgaatggca tcaagaaaat cacgcaatgt tttccgttga gcggtgagat ctggcaattc
     4501 cgataaagca gcttttgcca gctggatgtc atcatctgag taaaattttt tagaagccat
     4561 tggcactttc tctccgtttc atgttgaagt ctgattttat catcaaaaac tgactcctgg
     4621 cagggctgcg ccctgcctct gccgtctgta taagactatg atgcacaaaa ataacaggct
     4681 ataatggcct gaaaaacggg acaggatgtg caattgtaat accgtcacac gcgacgctat
     4741 tacaattgcc atctggtcag ggcttcgccc cgacaccccg taaggagcct gaagtgagtg
     4801 atagtgcggt aagaaagaaa agtgaggtac ggcaaaagac agttgtcagg acactcagat
     4861 tctctcctgt tgaggatgaa accatcagga aaaaagctga agattccgga cttactgtat
     4921 ctgcttacat ccgaaatgca gcactgaata agcgaattaa ctcccgtaca gatgatgctt
     4981 ttctgaagga gttaatgaga ctgggaagga tgcagaagca tctgtttgtt caaggaaaaa
     5041 gaaccggtga caaggaatac gcagaagtgc ttgttgccat aaccgaactt acaaatacat
     5101 tgcgtaaaca gttaatggaa ggttgaggat tctccggtga atgcagtcat tccgaaaaag
     5161 agaagggacg gcaagtcttc gttcgaagac ctggtatcct acgtctcggt ccgggatgac
     5221 atgaccgatg aggaactgaa tttatcttct tcttcgcagg ctgaacaacc gcaccggagc
     5281 cgtttcagtc gccttgttga ttatgcgaca cgtcttcgaa atgagtcatt cgtggcgctg
     5341 gttgatgtca tgaaggacgg ctgcgaatgg gtcaactttt atggtgtcac ctgctttcat
     5401 aactgtactt ctcttgaaac tgccgctgca gatatggagt acattgcccg gcaggcacac
     5461 tatgcaaaag atgacactga tcctgttttt cactacatcc tttcctggca gtcacatgaa
     5521 agcccgcgtc cggaacagat ttatgacagt gtacgtcata cgctgaaatc actcggcctt
     5581 gccgaccatc agtatgtctc tgccgtgcat actgatacag ataatctgca cgtccatgtg
     5641 gctgttaacc gggtacaccc tgaaacgggt tatctgaacc ggttatcatg gagtcaggaa
     5701 aaactcagtc gtgcctgccg tgagcttgaa ctgaagcatg gttttgctcc ggataacggt
     5761 tgctgggtcc atgcgccggg aaatcgtatc gttcgcaaaa ctgccgttga acgtgatcgc
     5821 cagaacgctt ggacacgcgg aaaaaaacaa acttttcgcg agtatgtcgc gcagacagcg
     5881 gtcgctggtt tacgcagtga acctgtaaat gactggttat ccctgcatcg tcgtcttgcg
     5941 gaagatggtc tgtacctgtc tcagatggac ggaaaatttc tggtgatgga tggctgggat
     6001 cgcaacaggg aaggtgtaca gcttgactcg tttggtccct cctggtgtgc agaaaaactc
     6061 atgaaaaaaa tgggtgacta cacgccagta ccaaaagaca ttttcagcca ggtggaagca
     6121 ccaggacgct ataatccgga cttcattgca gctgatgttc gcccggaaaa aatcgctgaa
     6181 acagaaagtc tgcagcagta tgcctgtcgt catcttggag aacgtctgcc ggaaatggca
     6241 cgggaaggac ggctggaaaa ctgccaggct attcaccgca cactggctga agcgggatta
     6301 tggatgcgtg ttcagcatgg tcacctggtt atctgtgacg gttatgatca taaccagact
     6361 cccgttcgtg ctgacagcgt atggtcactg ctgacgctcg acaatgtgaa tcaactcgat
     6421 ggtggctggc agcctgtacc aacagatatt ttccgccagg ttacgccaac agaacgcttc
     6481 cgtggtcgtc gtatggagag ctgtcctgcg accgataaag aatggcaccg tatgcgtaca
     6541 ggcacggggc cgcagggggc tatcaaacgc gaactgtttt ctgacaaaga aagtctgtgg
     6601 ggatacagca tcagccattg tagccctcag attgaagaaa tgatcacaca gggtgagttt
     6661 acctggcagc gttgtcatga gctgtttgca caacagggac tgatgctaca gaaacaacat
     6721 cacgggcttg ttgtcgttga tgcctttaat catgagcaaa cgccggtgaa ggccagcagc
     6781 attcatcctg atctgacact ggggcgtgca gagccacagg ccggaccttt tgtgagtgcc
     6841 ccagcagatt tgtttgacag ggtgcaacct gaaagtcgct ataacccgga gctggctgtc
     6901 agtgacaggt acggggtcag cagtaaacgt gacccaatgc tgcgccggca gcgacgtgaa
     6961 gccagagctg aggcccgtgc tgacctgcgt gcccgctatc tggcatggcg tgaacaatgg
     7021 cgtaaaccag atctgcgtta tggggaacgt tgtcgggaaa ttcatcaggc atgccgtctg
     7081 cggaagtcac acattcgtgc gcagtacgat gatccggcat tacgtaagct gcattatcac
     7141 attgcagaag ttcagcgaat gcaggcattg atcaggctga aagaagacat cagggatgag
     7201 cgacagaaac ttattgctga tgggaaatgg tatccaccct cttaccgtca gtgggtcgaa
     7261 attcaggctg ctcagggaga cagggctgct gtatcgcagc tgagaggctg ggattatcgc
     7321 gatcgccgta aagacaggtc acgcacaacg acgacagacc gctgtgtcgt gctttgtgaa
     7381 ccgggcggaa cgccggtata cggtaatacc ggtgatctcg aggcccgtct gcagaagaac
     7441 gggagtgttc gtttccgtga ccgtcggacg ggagagtttg tctgtacgga ttatggtgac
     7501 agggttgttt tccgtaatca ccatgaccgc aatgcgctgg cagataaact ggatttgatt
     7561 gcaccggtat tattcgggcg tgatccacgc atgggttttg aaccagaagg aaacgacaaa
     7621 caatttaacc aggtctttgc tgaaatggtt gcctggcata atgtgacagg ccgtaccggt
     7681 cacgaagact acaggattac gcgaccggac gtggatcatc accgtgaagg aagtgaacgc
     7741 tactaccgcg attatatcgc ggcgaacagc aacgatgacg catcactgcc tccgccggag
     7801 caggataaac gctgggaacc gccttctcca ggttaaaaaa aaggccgcgc cagaatccgg
     7861 cgcggccact gttcagtaca ttccgtccca gtccggacgg ttggtttttt tatgatgaaa
     7921 cccgtttaca ggacgccctg tcgggtggtt attgtcctgt ggaagcgtta acaggtttat
     7981 acatgctctt tctgctctca ctgatatctg attttcctct tttgtttcag gtagttctgg
     8041 tctgttcagc gtgatatagc gaattcgtcg ctcttcccgt gtggtcgttt taagcgtatt
     8101 caccgctgtt tcccacaagg cactttcacg tgcgttgata tctgtgtcgt actcttccag
     8161 cattgccata gccgcctgac gtcctgcaat gcgttgctgg aatgtatcca cgatcgtgtg
     8221 cggctctgtg cgaatttttc gtctttttct ggagggtttt gcagtcagga cgccaatgat
     8281 ggcactgaca ttttccggtg acggtattcg acgttgagta gtacggggaa caagacggcg
     8341 gagacggtcc aggcgtggcg catccacgct gatatacctg tttataacaa cgggatcgct
     8401 gctgcagctt ttctcatcgt cagggatgaa gaatgatgct gatggtacgg tttctccccg
     8461 gaaaatagaa aagaactcgc caggatgaag ttcaatcaac tcctgaacat ttatcttgct
     8521 ttccatcagg atgctgattt gtgggctgtc aatccatgat tcaccaaata tgccgtcctg
     8581 acgctgcagg ttgttcatcc tcgccctggc ttcttcaccg gcggcgcttt tcagcgttct
     8641 ggccgtacta ccctcgctga caatcctgcc ggcaaatttt gtccccgtgt tctgcatcag
     8701 tgttgcagta ttcgtggctg ttgtctgtcc ttctatacgt tcctggtcct gtgccatcaa
     8761 aatcagagca aaatccagtg aacgcacctg agtggcttcc acagcaatac ggtccgtata
     8821 gtatgccccc acttcatcaa ggaaacacag ataagggaaa cgccccttat attttttgac
     8881 ttccagtgca tcggaatcag ttccttcgag gcggtagccc aaatcacggg cgagaatcat
     8941 acttttctgg gtgataaaca ttcgtccaag agcagacgtt gtatgcgctg atgtatccag
     9001 agcggggatc attaccatca gaatcctgtc gctgtgaatg ctgtcacgga tatcgatatc
     9061 accggaatct tcagcaaaaa tatccccgaa tgcttccgtg aatgtgctga aagtttcaga
     9121 aaactgcccg gagagataag catgttgttt tctgggttct tccgtccagg ctgatggtgt
     9181 cctgaccagc gacaggtcga aaccgggaac gtcctgaagg tagttacgta acggtgcgat
     9241 agcttcttcc ggccactgat catcgaggcc acgacagtaa agtttggcca tcccttcgag
     9301 agtcatatgt tcacggagca tctgcagcga cattgttttg ccttcgcgca cacaccagaa
     9361 tttagtacca aataccagtg ccttattcat ggcaatggcc cgggactgcc actcgccccc
     9421 ctggacgttc tggggcagca ttgactgcat tgtctccgct gtgaatgcct cagtgctgta
     9481 acagaaggga ttccaggtat ttgactgagg acgcgttttt tcaccactca gaatgatttc
     9541 actgcgggat tttcctccgt tcatgaagtt aatgacttcc acatcgtctt cacgtccaaa
     9601 gcgtctggcc agataccaga tcgttcttgc cgtatcattc tgtgccttgc catcgacaag
     9661 ggtgaatcct cttgcccagc aaagtgggtt aatcgcccag gcaaagatgg tttccgtttt
     9721 accgccaccg gtggtggcga aaaacattat atggcgggtc agatcgtcca tactcagcca
     9781 taactccctg ccaatatccc tgacgcgttg ataaccaaca tagaatatgc cgctggccgg
     9841 tgattccaga atgacctcat actggaacag ggttggccag aagctgaaaa gacttctttt
     9901 aatcattctg tcctgtgacg ggtcagcaca ttccagcgtc atgggcatac gaagagggca
     9961 gcgccatcgc tgcagactaa ataccagcat tgttatcagt gtgaacaaca gacatgccgg
    10021 taatgcaagt ggccagataa aactggccac aagacttgcc accaggcaaa agccatagat
    10081 attgagactc tggagtgcat tccagacagg atttccccag gctgtacggt gtaaaagctc
    10141 ggggttaacg cgatgttcac tcatggccag actcgctgtc ggtggttttc aggaataaat
    10201 taagcgtgcc agtttcctgc agaattcctg taggcagatg ccagccggtg tcggacagca
    10261 cccatggggt accggcaaat ccgaacttac ggaaggcgac attgtttaca tcaattgcag
    10321 ctctggccgc tttcaggcat gtttcatctg caggcgttgt ttcttctggt ggcgtcatca
    10381 ttccgttatc tgcggaatac agtcggtgcc agccggcagc acgtttctga gcatcttttt
    10441 cacacagcag tggtgccaca cggtcggtgg attcctctcc accaataact gaaaccgggt
    10501 agataacgac attgaagtcg ctggccaggc gttttaaggc cggctccaga agtctgcagt
    10561 taggacaggc aggatcgaga aatgcgaaca gggttcgttc atgaccggat gacaggttga
    10621 tggtaaacat gccccgatcg gcgttccgct tcagaacagt agccagatta tgtcgcgcag
    10681 ctgcggcttc cggatttttg cttgtgattg ttttctccgg gacctggacg tcttttttcg
    10741 ggggaaccgg gagttgaatg ccttcattta atggcacagt gtgattcagg tgagggggta
    10801 gcagtcgtgg ctcacctgct gtctgtactg cttcaggcgt tgtatcatcc cagaatacac
    10861 gatcaacata gcggaacaga gagaagccaa tcgttgccat tacgaacagc acaacgctgg
    10921 cgattatgcc acggaacaaa ggtgttctgc tgaagcggcg ttccatcaga tattcaaggc
    10981 gcaggcagga atcaatagct gtaagtatct ggtcagcgtt atgctcctgt ttatcaatca
    11041 ggacggtttc tctgttacgg gtgcccatgg cgtcaaaggc tatatgaatc agttgattat
    11101 ttcgtgttac cagccctgac agggcatccc gtggaaggac ggcaataatt tctgtcctgc
    11161 catccggcca gattgcctgc acggaaatat gttttgcaat gcgtgttgca aagtactcga
    11221 ttgtcatttt cagttctccg gataatcaga attcgttgtc tgtagcaggt gtgcggggca
    11281 ggatgtcgtc ggtgagggac cagttggtca ggatgcggcg tttccggtcg cgatctggtt
    11341 gtggttcatc ccagataaga ccgagactga gcatatcccg ccgtaagcct tccaccgctt
    11401 catcaacaca gggttcaggg cagggaagac caaaacgcat ggcttcggcc tctgcacgtg
    11461 caacggccac cacgccagcg ccttcaataa atcctttcgt ggtattggcg cggtgtaatg
    11521 cataaaacag cgtcctgtcc acgcctttca gccaaatcca gttgggtggc gtaagccgca
    11581 ggtcatgtgc atacagccag accagcccac tgcggacgta gcgatgttgt ttcagccatt
    11641 gctgtgctcc gttgctttta atcactctct ggaaagctga tttggccagt gaataaacag
    11701 gggtacagaa tttcccgctg tcccgtttgc ttttaattct gcatgagagg ttcagtgtat
    11761 ccatgagctt taatgctgcc ttacgatcat ccaggaaata ctgaaggcca aatatggcaa
    11821 agagcgcttt ttcatgtggt gccatgtctt tccaggacgt taaaggttta cccaactggg
    11881 ccatgaaaca tcgtcttgca gctgcaacat cgagctgcat attggtgatg agagtatgct
    11941 gttcgacaaa tgcttccggg gtgagagcaa cccgccgttc aggtcgttta ccaccatgaa
    12001 agagtttttc cggatcacca tctgccagta caggggcgat agcaggggat aacgaagcaa
    12061 agatgtgtgg cagtcgggta atatctactg gtctgcgggt gaaacggctt tgtcccggat
    12121 gttgatacca ctcccacaaa caccagagtg tcacaggtaa gaggtacatc cacagaatac
    12181 ctatggtctg ttccatgacg ttaacccact ggctatagct tatgttggcg gcgttgttac
    12241 cggtcatggc aagcaggttg tatcgtggtg ctgcatagtt atgaaatggt ccccagtcga
    12301 ccagtcccca taaggtatga agaatcaggc agctggcgta aaccacttcc ggcaagaata
    12361 accatatgac gaatagcagc aggataagaa ggacaccaac agcgccccat atctgcatag
    12421 gatcttctgc gacaggctgt cgattgtaag acatgacaaa gtctccattt ctggaattta
    12481 aatcgaacgg aaaggttaaa tgtcacgaac ggtgcaatag tgatccacac ccaacgcctg
    12541 aaatcagatc cagggggtaa tctgctctcc tgattcagga gagcttatgg tcacttttga
    12601 gacagttatg gaaattaaaa tcctgcacaa gcagggaatg agtagccggg cgattgccag
    12661 agaactgggg
//
LOCUS       DAJTVJ010000120        12571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000120 DAJTVJ010000000
VERSION     DAJTVJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.120"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1213
                     /gene="narU"
                     /locus_tag="OO448_003334"
     CDS             <1..1213
                     /gene="narU"
                     /locus_tag="OO448_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6338467.1"
                     /translation="LNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWT
                     VFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQ
                     GSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVP
                     LLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAM
                     LAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFL
                     TLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQ
                     KEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTW
                     LVYGRRKFSQK"
     gene            1295..5035
                     /gene="narZ"
                     /locus_tag="OO448_003335"
     CDS             1295..5035
                     /gene="narZ"
                     /locus_tag="OO448_003335"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCR6338468.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5032..6576
                     /gene="narH"
                     /locus_tag="OO448_003336"
     CDS             5032..6576
                     /gene="narH"
                     /locus_tag="OO448_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6338469.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6576..7271
                     /gene="narW"
                     /locus_tag="OO448_003337"
     CDS             6576..7271
                     /gene="narW"
                     /locus_tag="OO448_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6338470.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7268..7948
                     /gene="narI"
                     /locus_tag="OO448_003338"
     CDS             7268..7948
                     /gene="narI"
                     /locus_tag="OO448_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6338471.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8027..8920
                     /locus_tag="OO448_003339"
     CDS             8027..8920
                     /locus_tag="OO448_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCR6338472.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9016..9861)
                     /gene="nhoA"
                     /locus_tag="OO448_003340"
     CDS             complement(9016..9861)
                     /gene="nhoA"
                     /locus_tag="OO448_003340"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCR6338473.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10034..10603
                     /locus_tag="OO448_003341"
     CDS             10034..10603
                     /locus_tag="OO448_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR6338474.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10607..10834)
                     /gene="pptA"
                     /locus_tag="OO448_003342"
     CDS             complement(10607..10834)
                     /gene="pptA"
                     /locus_tag="OO448_003342"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCR6338475.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10934..12069)
                     /locus_tag="OO448_003343"
                     /pseudo
     CDS             complement(10934..12069)
                     /locus_tag="OO448_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(12209..>12571)
                     /locus_tag="OO448_003344"
     CDS             complement(12209..>12571)
                     /locus_tag="OO448_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6338476.1"
                     /translation="KLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNH
                     WHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTNDKVFKAGLRRKMRK
                     AAMDRNYLASVLAGSGLS"
BASE COUNT         2978 a         3233 c         3225 g         3135 t
ORIGIN      
        1 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
       61 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
      121 acgccgatgg acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
      181 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
      241 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
      301 caaacaaggg agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
      361 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
      421 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
      481 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
      541 cgcctcaatt gacgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
      601 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
      661 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg
      721 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
      781 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
      841 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgactgc
      901 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
      961 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagaaagaag ccatcaccga
     1021 aacggcggcg gctctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     1081 gcaggcgttt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     1141 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     1201 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     1261 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     1321 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     1381 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaattg tgcgttccac
     1441 ccacggtgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     1501 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgaccagc ccaatcatga
     1561 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctctacagcg ctaaccgcct
     1621 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgtgaagctc tcaagcaaca
     1681 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcttaagcta
     1741 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac ttaaccaact
     1801 gattgccgcc gctaacgtct ggaccatcaa aatctatggt ccggatcgcg ttgccggttt
     1861 ctcgccgatc ccggcgatgt cgatggtgtc atacgctgcc ggaacgcgtt atctgtcgct
     1921 gttaggcggc acctgtttaa gcttctacga ctggtattgc gacctgccgc ccgcctcgcc
     1981 gatgacctgg ggcgaacaaa ccgacgtgcc ggaatctgcc gactggtata actccagtta
     2041 catcatcgcc tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     2101 cgaagtacgt tacaaaggca ccaaaactat cgccattacc cctgactact ctgaagtggc
     2161 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     2221 aatgggccat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     2281 ctactgccgc cgctatagcg acatgccgat gctggtaatg ctggaacctc gcgacgatgg
     2341 tagctacgta cccgggcgca tgatccgcgc gtctgacctg gtagatggac taggcgaaag
     2401 caataatccg cagtggaaaa ccgtggcggt taataccgca ggtgaattgg tagtgccgaa
     2461 tggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     2521 cggtaaggaa accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt
     2581 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     2641 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     2701 ggtggtcagc gtttatgatt tggtgctggc gaattacggc ctcgatcgcg ggctggaaga
     2761 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     2821 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     2881 ccataaaacg catggacgct caatgattat cctcggcgct ggcgtcaacc actggtatca
     2941 catggatatg aactaccgtg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     3001 aagcggcggc ggctgggcgc actatgtcgg tcaggaaaaa ctgcgcccac aaaccggctg
     3061 gttgccgctg gcctttgcgc tcgactggaa ccgcccaccg cgccagatga acagtacctc
     3121 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     3181 ttcaccgctt gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     3241 cgaacgcatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     3301 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     3361 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     3421 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     3481 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     3541 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     3601 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgttc tgcccaccgc
     3661 cacctggtac gaaaaagacg atatgaacac ctcggatatg catccgttta ttcatccgct
     3721 ttctgcggcg gtcgatccgg cgtgggaatc acgcagcgac tgggaaatct acaaaggtat
     3781 cgccaaagca ttttcgcaag tgtgcgtggg tcatcttggc aaagaaaccg acgtggtatt
     3841 acaaccccta ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     3901 gcgcaaaggc gaatgcgatc tgattccagg caaaaccgcg ccgaatattg tggcggtgga
     3961 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     4021 tggcaatggt ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     4081 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     4141 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     4201 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     4261 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     4321 ccccacatgg tccggtctgg aaagcgatca cgtctcctat aatgcgggat acaccaacgt
     4381 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     4441 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     4501 cgtcagtgag atgcgccaga tcccgccaaa cggcttcccg gaaaaagcac ttaacttcct
     4561 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     4621 gctctctcgc ggtggaccga ttgtctggat cagcgaaacc gatgcccgag aactgaccat
     4681 tgtcgataac gactgggtgg aagtctttaa cgccaacggc gcgctgacgg ctcgtgcggt
     4741 ggtcagccaa cgtgtaccgc cgggtatgac catgatgtat cacgcccagg aacgcattat
     4801 gaatattcct ggttcggaag taactggcat gcgcggcggc attcataact cggtcacccg
     4861 tgtttgcccg aaaccgacgc atatgattgg cggttacgcg cagctggcct ggggctttaa
     4921 ctactacggc accgtcggct cgaaccgcga cgaattcatc atgatccgca agatgaagaa
     4981 cgttaactgg ctggatgatg aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaata
     5041 cgttcgcaag tcggcatggt gcttaacctc gacaaatgta tcggttgcca tacctgttcg
     5101 gtgacctgta aaaacgtctg gaccggacgc gaaggcatgg agtacgcatg gtttaacaac
     5161 gtcgaaacca aaccgggcat tggttatccg aaaaactggg aagatcagga agagtggcaa
     5221 ggcggctggg tccgcgatgt gaatggcaag atacgcccgc gtctgggcag caagatgggc
     5281 gtaataacca aaatcttcgc caacccggtg gtgccgcaga ttgatgatta ctacgaacct
     5341 ttcaccttcg actacgaaca tttgcatagc gcaccggaag gcaaacatat ccctactgct
     5401 cgcccgcgtt cgctgattga cggtaagcgg atggacaaag tgatctgggg gccaaactgg
     5461 gaagaactgc tgggcggcga gtttgaaaaa cgtgcccgcg accgcaactt cgaggccatg
     5521 caaaaggaga tgtacgggca gtttaaaaac accttcatga tgtacctgcc gcgcctgtgc
     5581 gaacactgcc tcaatcccag ttgcgtggcg acctgcccaa gcggcgctat ctacaaacgc
     5641 gaagaagacg gcattgtgct gattgatcag gataaatgcc gtggctggcg tttgtgcatt
     5701 agcggttgtc cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc agaaaaatgt
     5761 atcttctgtt atccgcgaat tgagtcaggc caacccaccg tgtgctcaga aacctgcgtg
     5821 ggtcgcatcc ggtatctggg cgtgctgctt tacgacgccg accgcattga ggaagcggcg
     5881 agcaccgagc gcgaagttga cctctatgaa cgtcagtgcg aagtgttcct cgatccacac
     5941 gatccctcag tgatcgagga agccctgaaa caaggtattc cacaaaacgt gattgaagct
     6001 gcccagcgtt cgcctgtcta caaaatggcg atggactgga aactggcgct accgctgcac
     6061 cctgaatatc gcaccctgcc aatggtctgg tacgttcctc cgctgtcgcc gattcagtcc
     6121 tacgcagatg cgggcggttt gccgaaaagc gaaggcgtgc tgcccgccat cgaaagcctg
     6181 cgtattccgg tgcaatatct cgccaatatg ttgagtgccg gcgataccgg tccggtactg
     6241 cgggcgctga aacggatgat ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc
     6301 gttactgata ctcgtgccat cgacgaagta ggcctgagcg tcgcccaggt cgaagaaatg
     6361 tatcgctatc tcgccattgc caactatgaa gatcgttttg ttatcccgac cagccatcgg
     6421 gaaatggcgg gcgatgcctt cgcagaacgc aacggctgcg gttttacctt tggcgacggt
     6481 tgccacggtt cggacagtaa attcaacctg ttcaacagta gccgtatcga tgccatcaac
     6541 atcaccgaag tgcgcgacaa agcggagggc gaataatgca gatcctcaaa gtgatcggcc
     6601 tgttgatgga gtatccggat gagctgttgt gggaatgcaa ggaggacgcg ctggcgttga
     6661 tccgccgcga cgcgccgatg cttacggatt tcacccacaa cctgcttaac gcgccgctgc
     6721 tggataaaca ggccgaatgg tgcgaagtgt ttgaccgcgg gcgcaccacg tcgctgctgc
     6781 tgttcgaaca tgttcatgcc gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg
     6841 cggagtatga aaaggtcggc ctgcagctgg attgtcggga actgcccgat tatctaccgc
     6901 tgtatctgga gtatttaagc gtactgcctg acgatcaggc gaaagaagga ttgctcaatg
     6961 ttgcgccgat cctcgccctg cttggcggtc gcttaaaaca acgcgaggca ccgtggtacg
     7021 cgttgtttga tgctctgctg caactggcgg gaagtatact ttcaagtgac agtgtcacaa
     7081 aacaagtaaa cagcgaagag cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag
     7141 aggaacaggt gaagtttatt gaagataacg ccacggcgtg tgacagctcg ccgcttaatc
     7201 aatatcagcg acgctttagc caggatgtcg cgccgcagta tgtcgacatc agtgcgggag
     7261 gtgggaaatg attcagtatc tgaacgtctt tttttacgat atctacccgt acatttgtgc
     7321 gacggtgttt ttcctcggca gctggctgcg ctacgactac gggcagtaca cctggcgcgc
     7381 ctcctcaagt caaatgctcg ataaacgcgg gatggtgata tggtcgaatc tgttccatat
     7441 cggcattttg gggattttct tcgggcactt gttcggcatg ttaacgccgc actggatgta
     7501 cgcgtggttt ttgccagtgg cagcgaaaca actcatggcg atggtgctcg gcggtatttg
     7561 cggtgtgttg acgttgattg gcggcgcagg actgctgtgg cgcagtctga caaatcagcg
     7621 cgtgcgtgcc acttccacta cgccggatat catcatcatg agcattttgc tgatccagtg
     7681 tctgttgggg ctaagtacca taccgttttc agcacagtat cctgatggta gcgaaatgat
     7741 gaagctggtg ggttgggcgc aaagcattgt cactttccgt ggtggatcgt cagagatgct
     7801 caacggcgta gcgttcgtct tccgcctgca tctggtgttg ggaatgacca tcttcctgct
     7861 cttcccgttc acccgactgg tgcatgtgtg gagcgcgccg tttgagtact ttactcgtcg
     7921 atatcaaatt gtgcgttcgc ggcgataatt tcaccctggc cggatagccc ttcgctctcc
     7981 ggccacttgc aggctatgat agttctccag tcacaggaga acgttaatga aaccgcaggt
     8041 gtaccacgtc gatgctttta cctctcaacc atttcgcggc aattctgccg gtgttgtttt
     8101 ccccgccgat aatcttagcg aagcacaaat gcagcttatc gcccgcgagt taggccattc
     8161 ggaaactgct tttctgctgc acagcgacga cagcgatgtg cgcatccgct actttacgcc
     8221 aacggttgaa gtgccgattt gcggtcacgc gacagtagct gcgcactatg tacgtgcgaa
     8281 ggtgttaggt ttaggaaatt gcacgatctg gcaaacatcg ctggcaggaa aacatcgcgt
     8341 gactatcgaa aagcacaacg atgattatcg tatttcgctg gaacaaggta cgccgggctt
     8401 tgaactgccg ctggagggtg aagtacgcgc ggcgattatc aacgcattgc atcttactga
     8461 agacgatatt ctgccgggtt tgccgatcca ggtcgcgacg accgggcatt ctaaagtgat
     8521 gatcccattg aagccggaag tggatatcga cgctctttcg cctgacctta atgcgctgac
     8581 cgctatcagt aaacagattg gctgcaatgg tttcttcccg ttccagatcc gtccaggcaa
     8641 aaacgaaacc gatggtcgta tgttctcgcc tgcgattggt attgtggaag atccggtgac
     8701 tggcaatgcc aatggcccga tgggtgcatg gttggtacat cacaacgtat tgccccacga
     8761 tggcaacgtg ttgtgtgtta aaggccatca ggggcgcgca ttggggcgtg acggcatgat
     8821 tgaagtgacg gtgacaattc gtgataacca accggaaaaa gtcaccattt caggcacagc
     8881 ggtgattttg tttcatgccg aatgggcaat tgaaatctga aatcgttgcc tgatgcgctt
     8941 cgcctgtcag gtctacaacc gggctaatgt atgccggata aggcgtttac gccgcatccg
     9001 gcaacctgac ataaattatt ttcccgcctc cggatgcgta tcaaacgccg ccatcaccag
     9061 cgctaactca tccacagtaa agccatgttt cgcatcatcc acgcccagac caaactgttc
     9121 ctgcatcacg gcatataacg acgccacatc cggtagattt cgctgctcca ccgcgtgccc
     9181 attttcataa tgggtaaaat gaaaattggt cagtgtcagc ttgccgccgt ccggcaaatg
     9241 gcggcacatc agcaaatgat ggcgaaaatg cgactgcggc cagtgcgccg accagaaatt
     9301 gcccatcaca taatcgcttt gttgctgctc gcagagatcg aagcggtaca tcggctgcca
     9361 atgctgatga tgattaaact gcaacaccca atcatcaccc tcctgcaaca accgatactc
     9421 tccgtgtggc gtggtctgca cgagatcgga aactaaacga atcggcgcgg ttagcgtctg
     9481 cccaccgaaa ccgacatcag caatccattt ttccccttcc agttccacca acagcaaacg
     9541 atgggtgcgc ggcggtaatg ctggcggatt tgataacact acgcgcccta acaagctgcg
     9601 aacgttaaac cccagctcgc gtaacacccg ctcaaacacg ccattctgct caaaacagta
     9661 accgccacga cgggctatca ccagtttctc ttccagcgat tgatcatcaa gctgtatttc
     9721 cctcggcagc aaaacgtcaa ggttttcaaa cggaatggtg caattgtgtt tcaggtgcaa
     9781 tgcacgaagc gtatcaatat tgaccgcagc agctcccgac cagttaatac gggcaaaata
     9841 gtgattcaga atgggcgtca tcaggcgttt cccttagtgg ttttctcaac ttataacctg
     9901 gaatagacgc cgcaccggct attttgtgcg atcgcggaca tgcatcacgt ttgcaaatcg
     9961 ccaatcccgt taatttcgtt acttattgcg ttacggacta cgcttaaaaa aaccttcttc
    10021 gcgaggcgat accatgagcc gattcatccc catagaatta caccatgcca gccgtctgtt
    10081 aaatcatggc ccaactgtct tgatcaccag tttcgatgag caatcccagc ggcgtaacat
    10141 tatggctgca gcctggtcaa tgccggtgga gtttgaacct ccacgcgtgg caattgtggt
    10201 agataaatcg acatggacca gggagttgat tgaacgtaac ggtaagtttg gcatcgttat
    10261 cccgggcgtt gcagcaacta actggacgtg ggcggtggga agtgtgtcgg tgcgtgaaga
    10321 agataaattt aattgctatg gcattccggt ggtgaaaggt ccggtgcttg gtttgccact
    10381 tgttgaagag aaatgcctgg cgtggatgga gtgccgcctg ctacctgcga cttctgcgca
    10441 acaaaaatac gacacgctgt ttggcgaggt ggtatcagca gcggcagacg cacgggtatt
    10501 tgtcgaaggc cgctggcagt ttgatgatga taagctcaat acgttgcatc atttaggtgc
    10561 tggcacgttt gttactagcg gcaagcgtgt tacggcaggt taagcattac atgctgtaac
    10621 cgggtttctt tatcaaagcc tccatttggg gctcgatttc ggcatcccag atagcttgcc
    10681 aagattctgg ttgaatctgc tgtagagcaa tgcttatcga gctatcttta ctgttcagat
    10741 ggcgaataat aacgtcggta atatctgcag caagtgctgc tttttgttgt tcgtccagtt
    10801 cacgcggaaa gcatttaatg tcgatgtgcg gcatgattac gctccttttg aaagatgtca
    10861 gtaaaattaa cacacattgt gaatgtcatg tgtccggtaa agcgtttata cgacaataat
    10921 gcccagggca agattacgaa agcccgctcc ccgtaaggac tgacgccagg tagtttctgc
    10981 ccatggctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
    11041 aaatattaat agcaatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
    11101 ttttgcagtc gtcttcattc attaccacgt ccagacgcca atgcagctta ttctccacgt
    11161 gccagtggtt tcggattgct gtggcgaact tctctgcggt taaatcagca gaactgatat
    11221 aatatctgac cgtcatttct ggctctttct tttgttctgc tattatggac cgaaaggaga
    11281 ctgccacgca taatttcttc agccctttcc attcaaacgt gaaatcaata agttcatcag
    11341 ggacatcgca aacaatatga agacggattt cttctctgcc gtgactcttt tcactaattg
    11401 cgtaactgtc attctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt
    11461 tatttagccg cccctggttt ccttttaccg cgaataaata atcacctccc tgtttttgta
    11521 tcttctctgc aatatctttc tggcaaccca tcgcatcagt tgtgatgatt tttcctttaa
    11581 tatccagcat gttaagaagt tcaggaatgg ctgtaatctc attggatttc tcatccgtct
    11641 taatctgccc gagaatcaga ctgtgcattg ttgagaacgc actaatgaca tgaattgctc
    11701 ccctgcggca ctcttgtcat aagagtgccg gagcgttttt ccatcaattg caatgacgtc
    11761 tttatcatct gaagaatggc agtcacgcat ccagttaata aagcactcgt gaaattttgc
    11821 aggactgata caggatacaa ctctggcgat ggtatcgtga acaggaatac cattttcaaa
    11881 atcaccatat tgttttaaaa aatcgagatg tgtttcccca aaatcctcta tatcttccca
    11941 gccttctgca ccagaaataa cggcacaaat agtcaacagt agaatatccg ataatttatg
    12001 ttccactttc caggcttgtt tttaatcggg gataatagaa atatgttcca tcaatttttt
    12061 aagttccatt ttgttctcct taattatgta aggagtattt gatcatgtat aagcaataaa
    12121 aaacagcttc aggtaataag gaatatctca atttttaaac ataaaatgcg aattatttag
    12181 tacaaaaagt gaactcctca gggcaagatt acgaaagtcc gctccccgca aggactgacg
    12241 cgagatagtt tctgtccatg gctgcttttc gcatcttacg tcttaaccct gccttgaata
    12301 ccttatcatt cgtcaaaaca ttaatagcaa tgtgccgtat ccctgaaaat aattctgctg
    12361 catttcctct tcttattttg cagtcgtctt cattcattac cacgtccaga cgccagtgca
    12421 gattattctc cacgtgccag tgatttcgaa tcgctgtcgc aaacttctca gcggttaaat
    12481 cagcagaact gatataatat ctgaccatca tttcgggctc tttcttttgc tctgctatta
    12541 ttgagcgaaa ggagactgcc acgcataatt t
//
LOCUS       DAJTVJ010000121        12487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000121 DAJTVJ010000000
VERSION     DAJTVJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.121"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="OO448_003345"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="OO448_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6338477.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="OO448_003346"
     CDS             433..621
                     /locus_tag="OO448_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6338478.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="OO448_003347"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="OO448_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6338479.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="OO448_003348"
     CDS             816..989
                     /locus_tag="OO448_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6338480.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="OO448_003349"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="OO448_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OO448_003350"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="OO448_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR6338481.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="OO448_003351"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="OO448_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6338482.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OO448_003352"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="OO448_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6338483.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="OO448_003353"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="OO448_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6338484.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="OO448_003354"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="OO448_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OO448_003355"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="OO448_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6338485.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OO448_003356"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="OO448_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6338486.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3011 a         3203 c         3269 g         3004 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggtat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtt
//
LOCUS       DAJTVJ010000122        12451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000122 DAJTVJ010000000
VERSION     DAJTVJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.122"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..191
                     /gene="bdm"
                     /locus_tag="OO448_003357"
     CDS             <1..191
                     /gene="bdm"
                     /locus_tag="OO448_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR6338487.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            293..430
                     /gene="sra"
                     /locus_tag="OO448_003358"
     CDS             293..430
                     /gene="sra"
                     /locus_tag="OO448_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR6338488.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            587..2284
                     /gene="maeA"
                     /locus_tag="OO448_003359"
     CDS             587..2284
                     /gene="maeA"
                     /locus_tag="OO448_003359"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6338489.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2418..3420
                     /gene="adhP"
                     /locus_tag="OO448_003360"
                     /pseudo
     CDS             2418..3420
                     /gene="adhP"
                     /locus_tag="OO448_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3566..3850
                     /locus_tag="OO448_003361"
     CDS             3566..3850
                     /locus_tag="OO448_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6338490.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OO448_003362"
     CDS             complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OO448_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6338491.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OO448_003363"
     CDS             complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OO448_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6338492.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OO448_003364"
     CDS             complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="OO448_003364"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8081..8083),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6338493.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8900..9781
                     /gene="yddG"
                     /locus_tag="OO448_003365"
     CDS             8900..9781
                     /gene="yddG"
                     /locus_tag="OO448_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6338494.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10040..11140
                     /gene="ompC"
                     /locus_tag="OO448_003366"
     CDS             10040..11140
                     /gene="ompC"
                     /locus_tag="OO448_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6338495.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            11304..11786
                     /locus_tag="OO448_003367"
                     /pseudo
     CDS             11304..11786
                     /locus_tag="OO448_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11901..>12451
                     /locus_tag="OO448_003368"
     CDS             11901..>12451
                     /locus_tag="OO448_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6338496.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHN"
BASE COUNT         3231 a         3105 c         2968 g         3147 t
ORIGIN      
        1 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcaag
       61 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata
      121 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
      181 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
      241 ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc
      301 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
      361 agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc
      421 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      481 ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga
      541 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      601 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      661 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      721 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      781 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga
      841 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
      901 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
      961 gtttatctct taccagaacc gccacaatat ggacgatatt ctgcaaaacg taccgaacca
     1021 taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
     1081 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
     1141 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacaactgct
     1201 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
     1261 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt
     1321 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1381 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1441 aagccgagcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1501 agcgggatgc ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga
     1561 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat
     1621 gccgaacctg ctgcctttcc agaccaaact ggtgcagaaa cgcgaaaacc tcagtgactg
     1681 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1741 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1801 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     1861 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca cgggcagccc
     1921 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     1981 tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga
     2041 gatgctgatg tcggcaagtg aaacgcttgc acagtattcg ccgctggtgc tgaacggcga
     2101 aggtctggta ctaccggaac taaaagatat tcagaaagtc tcccgtgcaa ttgcgtttgc
     2161 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg aagctttgca
     2221 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     2281 ctaagcctgc gcccggtagt gaaggctacc gggcaatttt cctctccctt tttcagatct
     2341 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2401 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2461 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2521 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa accgacgtaa
     2581 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2641 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2701 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2761 gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg tctggactcg
     2821 gcggcggcca gcagcattac ctgtgcgggg gtcaccacct acaaagccgt taagctgtca
     2881 aaaattcgtc ccgggcagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc
     2941 ctgcaatacg caaagaatgt ctttaacgcc aaagtgatcg ccattgatgt caatgatgag
     3001 cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac
     3061 gccgccaaaa ttgtgcagga gaaaaccggt ggcgctcact ctgcggtggt aacagcagta
     3121 gctaaagctg cgtttaactc ggcagttgat gctgtccgtg caggcggtcg tgttgtggct
     3181 gtcggtctgc cgccggagtc tatgagcctg gatatcccac gtcttgtgct ggatggcatt
     3241 gaagtggtcg gttcgctggt cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc
     3301 gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc
     3361 tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgtcactaa
     3421 gaggtctttg ctgcgactcc catgttcggg tcgcagcatc gcacactctc caacatgaaa
     3481 tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc ccagagaagg
     3541 aacgctactg agagtaaaga cactaatgaa aatggccaat catccccgcc cgggggacat
     3601 tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca gagcaatgga
     3661 aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga cgccagaaat
     3721 ggcaattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa
     3781 cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgattac gccgtctggt
     3841 tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat
     3901 tgcctgatgc gctacgctta tcaggcctac acgttcactt caatctattg tatttgaagg
     3961 gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc cacgttgtct
     4021 acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca aaagagatta
     4081 tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg
     4141 atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgaccc
     4201 tttcacccag aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc
     4261 agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact gcgcaaagta
     4321 cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca tgatcgacca
     4381 gaacatcatc ttttgcccgg cgttgtactt accaacatcc gccactttgt gctcattgcc
     4441 ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg
     4501 atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc cgaagaacgg
     4561 gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg
     4621 gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg
     4681 acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca tgatgatcct
     4741 cctcgtcatc gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg
     4801 caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta
     4861 ccgaggtgtc gatcttcgga tctttcggca gaccgtaata cagctccggc tgatcggcgt
     4921 gatgcagcac gtacataacg tgcgtaccac cgaccccttt cgggttgtag acgccagcat
     4981 gttcgtaacc acgcgctttc agtttcgcca cgcgctcttc cgccagctcc agcatctcct
     5041 tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc
     5101 cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga
     5161 ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg
     5221 actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac gggcacgcct
     5281 tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc cactccagct
     5341 tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg
     5401 cggggttatc gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg
     5461 aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata agttttgcga
     5521 cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt
     5581 tgataatatc ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttga
     5641 ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag
     5701 tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca
     5761 ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt accaccgcca
     5821 ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta ttaatgcctt
     5881 tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga
     5941 gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc gtacccacat
     6001 acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg
     6061 ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca atcggctcgt
     6121 agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt
     6181 ccggctgcat gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa
     6241 tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc ggatcccacg
     6301 gtttaccatt gatatccgcc gaagcacggt tgtagagcac gcgacggttg agcggccacg
     6361 cccaggccca tcccagcgta ttccccagac cggatgggtc ggagttatcg cggttagcca
     6421 tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat gcggttgtac
     6481 cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc
     6541 cattagcgtc atagagatct tccagcgcat agccgttatt ctctttcgcc acttcgtcag
     6601 attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt
     6661 taccaccttc ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc
     6721 cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac
     6781 cggagttagc gatagaacca tcttcttcgg caaagcaggt cgaaggcaga cggaatactt
     6841 cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag
     6901 aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca
     6961 ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aagtaaccgg
     7021 tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact
     7081 tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca tagaaagatt
     7141 tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga tcagccagcg
     7201 tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggaa
     7261 gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcagc ccctgaatgt
     7321 tggagtgacc acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagca
     7381 actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc gtccagccca
     7441 gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca
     7501 ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc
     7561 gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt gtttcatcgc
     7621 gttttgcata gccgttttca tcgaactgat agttccagga cgacttatca tactggcgtt
     7681 tttcagcgtc gtagccgctg aacaggccgt cttcgaaagc aaaatcatca cgcaccagca
     7741 ggctggcgtt ggtgtaatgt ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt
     7801 agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat
     7861 ccgccacaga agcggtacgc gtaaaacgag gatcgacaac gatcaaggtt gcatcgttgt
     7921 tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc
     7981 ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc
     8041 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt
     8101 ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg gtttcgttgc
     8161 tggcaccgga ggcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct
     8221 cgttcttttc aataaagtta gcgtcacggt cagctttcat cagcttcgca atacgggaga
     8281 atgcttcttc ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt
     8341 agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc ggcccctttt gggcacagcg
     8401 caccacggct taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt
     8461 ttttcgcgcc atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc
     8521 aggtgttacg gatctcttta gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt
     8581 gcttcggggc aaagcccaat gccgctaccg ttgttccagc cataccgccc gcgcagattt
     8641 taaaaaattg tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg
     8701 cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgta
     8761 tgccacaaaa taatccgcct gcgcaaatta acctacctca atagcggtag aaaaacgcac
     8821 cactgcctga caggccagtt aaaaaaatgc tataaaattc agcttaattt ttaacggcaa
     8881 gagagacaaa acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat
     8941 cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc agcgaggggc tcggtccggt
     9001 cggcggcgca gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt
     9061 tccgcgtatt cggcagattc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag
     9121 ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg acccgtcatc aggcgattga
     9181 agtgggtatg gtgaactatc tgtggcccag cctgacaatt ctctttgcca ttctgtttaa
     9241 tggtcagaaa accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg
     9301 ctgggtgtta ggcggtgaga atgggttaca ttatgatgaa atcatcaata atatcaccac
     9361 cagcccattg agttatttcc tggcgtttat tggtgcgttt atctgggcag cctattgcac
     9421 agtaacgaat aaatacgcac gcggatttaa cggaattacc gtttttgtcc tgctaacggg
     9481 agcatgtctg tggatttact attttctcac gccacaacca gaaatggtat ttagcacgcc
     9541 cgtcatgatt aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa
     9601 tgtcggtata ttgcatggca atgtcaccat aatggcggta ggctcgtatt ttacgcctgt
     9661 actttcctca gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca
     9721 aggcgcgcta atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggtta
     9781 aaataattgc cgggtctacg gcccggcagt taatcagata aattccgaca atattaaaaa
     9841 ataaatttca aataccagta attcacctta tatctcattt cgacttcgat cacatatcga
     9901 taaataatat tatttcatct atttttctca ttgtttttaa tcaccattct attttaaaaa
     9961 cagtatgtca tttataaatg acgccgttta aaattcgttt agaaaatacg tttaacgata
    10021 attaaaagga ttctgttaaa tgaaattaaa aatagttgcg gtggttgtaa ctggtttgtt
    10081 agctgcgaac gtagcacacg ctgccgaagt ctataacaag gatggtaata aactcgacct
    10141 ttatggcaag gttaccgctc tacgttattt tactgatgat aagcgtgacg atggtgataa
    10201 aacttatgcc cgtctcggct ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt
    10261 tggtcactgg gaatatgatt ttaaaggcta taacgatgaa gccaacggct cgcgcggcaa
    10321 caagacccgt ctggcctatg ctggtttaaa aattagtgaa tttggctctc tggactatgg
    10381 tcgtaactac ggtgtcggct acgacattgg ttcatggacc gatatgttgc cagaatttgg
    10441 tggcgatacc tggagtcaga aagatgtctt catgacatac cgtaccaccg gtctagcaac
    10501 ctatcgcaac tacgatttct ttggcttaat tgaagggctg aactttgccg cgcaatatca
    10561 aggcaaaaat gaacgtactg acaacagtca tctttatggt gctgactaca cgcgtgccaa
    10621 cggtgacggt ttcggtatct cctcaactta tgtttatgat ggctttggta tcggtgcggt
    10681 atataccaaa tccgatcgga caaatgcgca ggaaagagcc gctgctaatc ctctcaatgc
    10741 ctccggtaag aatgcagaac tatgggccac aggtataaag tatgatgcca acaacatcta
    10801 ctttgcagct aattacgctg aaacattaaa catgaccacc tatggggatg gttatatctc
    10861 taacaaagca caaagttttg aagtggtggc gcaatatcaa ttcgacttcg gcttgcgccc
    10921 atcactcgct tacctgaaat cgaaaggcag agatctgggc cgctacggcg accaggacat
    10981 gattgagtat atcgacgttg gtgcgacgta tttcttcaac aaaaatatgt cgacctatgt
    11041 tgattataaa atcaacctga ttgatgaaag cgactttacc cgtgccgtag atattcggac
    11101 cgataacatc gtcgctacgg gcattaccta tcagttctaa cgtcctctcc tgaccagccc
    11161 ttctctgggc tggtttttga tttaagtgct gctgtatgac tccttaagta aaaatgagca
    11221 atttttgata tttctggtca ttcacctcaa ttaatattat tccatatcct ccggggacat
    11281 cttactataa tatttattga tgagtgatta tgaccaatat taatacagct tgtgtaaaaa
    11341 ataatgccag ttatcaactt aataacgcat tacctaacaa agaaaccatc tccagcaatt
    11401 tttgtgaacg actagaacaa tggggtaata aatcggttaa taatggtgaa gaaagagcaa
    11461 ttgccgtgga gcgaattaaa gaagcttaca attcgaacat ggcatctctt gatttgtcat
    11521 atcttgattt gagtgaatta ccgcctatcc cctcgacagt taatacgtta aatctggaaa
    11581 acaattgtct aacttgtctt gacttagctg acaatgccag tctcgttaat atcaacctca
    11641 gcgttaataa aattaacacg ataacctttc caaaagaatc aaaactggaa aacatttata
    11701 ttgatcataa caatctggaa agtttagatt taaaaaatca gcattcattg gttaatctgg
    11761 aagcgcaaaa taacaaactg acaaaaatta atatttctga tagttataaa ctgaaatttc
    11821 ttaatcttga ttataacaag ctagcatcgc tggatgtctc ccggcaagaa tctctgattg
    11881 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
    11941 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
    12001 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
    12061 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
    12121 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
    12181 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acatctatca
    12241 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
    12301 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
    12361 ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat aggctttcat
    12421 cagttactta ttttagtgcg gcacataatc a
//
LOCUS       DAJTVJ010000123        12232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000123 DAJTVJ010000000
VERSION     DAJTVJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.123"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..201)
                     /locus_tag="OO448_003369"
     CDS             complement(<1..201)
                     /locus_tag="OO448_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCR6338497.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFD"
     gene            complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OO448_003370"
     CDS             complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OO448_003370"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6338498.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OO448_003371"
     CDS             complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OO448_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6338499.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OO448_003372"
     CDS             complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OO448_003372"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6338500.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OO448_003373"
     CDS             complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OO448_003373"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6338501.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OO448_003374"
     CDS             complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OO448_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6338502.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OO448_003375"
     CDS             complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OO448_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6338503.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OO448_003376"
     CDS             complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OO448_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6338504.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OO448_003377"
     CDS             complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OO448_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6338505.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OO448_003378"
     CDS             complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OO448_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6338506.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OO448_003379"
     CDS             complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OO448_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6338507.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OO448_003380"
     CDS             complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OO448_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6338508.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11856..12206)
                     /gene="flhD"
                     /locus_tag="OO448_003381"
     CDS             complement(11856..12206)
                     /gene="flhD"
                     /locus_tag="OO448_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6338509.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2792 a         3422 c         2988 g         3030 t
ORIGIN      
        1 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
       61 aaaccagata acacacacgc ccactaacaa aatcagcaac gaggtcagtt cacgggaacg
      121 cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt
      181 gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagct taaatgatgc
      241 cagagcgcaa agcgttcaat ggtttgagta aggggcaaaa caggcgggat ttagggcttt
      301 tgctgccaca catcaagcat agtgtgcgtt tgtcggatgc ggcgtcatcg ccttatcaga
      361 ccgcctgata tgacgtggtc acgccacatc aggcaataca aatcaaaatc caagactatc
      421 caacaaatcg tccacctgat cctgactggc taccacgccg actttgctgg tatcgacctg
      481 aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt ccgggatgtt
      541 ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca tcatccgctt
      601 aatgaccagc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc
      661 gttagtaaag ctggtatgcg cgggtacatc tgccagaaat tgtcgtgtat ctgttaccag
      721 ttcacgggca tcggcaaggt caatcggatc ggcaaaccag tcatcccaac gttgggttaa
      781 cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa cactgttcag
      841 cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat cgcgcgcatc
      901 ggggatggct tccgccgctt cggcaatggc ctgatccagc cccagttccc gcaaactgtc
      961 gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc
     1021 agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt ttctcaaaga
     1081 ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg
     1141 cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc accattaaca
     1201 ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt cagcaattcc aggccatcca
     1261 tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc aacttattga
     1321 gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct ttcagcaggt
     1381 tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta agttctttat
     1441 ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa tttttgccaa
     1501 catttgctgg cttacctggc taagatcgac cacttcgcag acaccaccca tattgatggc
     1561 ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc
     1621 cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg tcaggatcac
     1681 cccaaccgca ttacgccccg cctgtttggc gacagaatgg aacaacacat ctaccgaagg
     1741 ccgatgacgg ttaaccgccg ggccgtcgtg aattttgatt tgataatttg cgccactacg
     1801 cgccagttcc atatgccgat cgcccggcgc aatataggca tgccccggca agacacgttc
     1861 tccgtcttcg gcttctttaa ccccgatctg gcaaagctta ttaagtctgt cggcaaaaga
     1921 gcgggtgaaa ccgggcggca tatgttgggt aattaacaat gccgggctgg aaagcggcaa
     1981 cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa tcgcaatcag
     2041 tttttcagaa ctcaacaacg gacccgcctt cagcgttgtc ggtgctgaca atggcttatg
     2101 tgctgcaagg ctcgcctttg ctgccgtacg caccttttca gcaatcattt cgctatacgc
     2161 cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctattg cccccagctc
     2221 cagcgcgcgc agcgtgactt ctgatccttt gccggtcaag gaagaaacca tcacaacggg
     2281 cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc gcggcatttc
     2341 aacatccagc gtcagcacat cgggattgaa tttcttaatc aagtcacgcg cgaccagcgg
     2401 atcaggcgcg gtcgccacca tttccatgtc gctatggctg ttgatgattt ctgtcatgat
     2461 ctggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca tcgttaatcc
     2521 ttacttagcg catacaccgt ctgaccacgc agcgtgaagc ggcgctcaag gtggctaaag
     2581 ttttcagagt aacccgcaaa cagcaatccg tcgggtttaa ggagcggaac aaagcggcgc
     2641 aaaatctcct gctgggtatt ttgatcgaag tagatcatga cgttacgaca gaagatcgca
     2701 tcaaacggcc ccggcacggt gtactgtttc gccagtagat tcagcggggc aaaatcaaca
     2761 tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt ccctcgcatg
     2821 aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg ataaataccg
     2881 cttctggctt tttccagcac ttcggtgtcg atatcactgg caaacacttt ccagcgtccg
     2941 ggcgcggtgc ccaatgtgtc agccagcgtc atcgcaatgc tgtacggctc ttcgccggtc
     3001 gaagccgccg cgctccatac gcgatactcg ccagaacggc gatgtgcgtg atccgcgagc
     3061 agagggaaat ggtgtgcctc acggaaaaat gccgtcagat tcgtggtcag cgaattgata
     3121 aacgcctgcc actcaccgct gtgctgatta gattccagca agttcagata atgaccgaaa
     3181 tccgtcagtc ccagcgaacg caaacgacga accagtcggt tgtaaaccat gtcgcgttta
     3241 tggtcagcca gaacgatccc ggctcgttga tagatcaatt gacttatccg ccgaaaatgc
     3301 gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg cccacagggc
     3361 agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc aatttgtaac
     3421 tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc cacacgcgac
     3481 gaaagatggt cggcctgatt cgccagttgt tccgttgcca ccgccgcttc ttctaccagc
     3541 gaggcgttct gctgagtcac ctgatccatc tggctgacag cctgtgcaac ctgctcaatc
     3601 ccccgctgtt gttcttccga cgcagaggca atttctccca taatgtcgtt cacgcgggtc
     3661 accgaactga caatatcgat catggtcgcg gcggcgttat tcaccagttt cgaaccctgc
     3721 tggacacgat tgactgactc ttcgatcagc cctttgatct cttttgctgc ctgcgcgcta
     3781 cggctggcaa gattgcgcac ttcacctgcc actaccgcaa aaccacgccc ctgctctccg
     3841 gcgcgagccg cttccactgc cgcattcagg gccagaatat tggtctggaa agcaattccg
     3901 tcgataacgc tgataatgtc gccaattttt tgcgagctgg tggcgatctc ctgcatggtg
     3961 tgagtcatgg tactgacctg aacaccgccc gcctgcgccg ttgtcgcggc attttttgcc
     4021 agttccgacg cctgtcgtgc gttatcggcg ttttgcccta ccgtggcggt taattgctcc
     4081 atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt cggtacgact tgagagatcg
     4141 ttattgcctg cgacaatctc cgcgatacca atgtgcatct cctggcttcc cttacgcaca
     4201 tcacttaccg tcccacgcaa agcctgctgc atggtcttca gactggcaaa aatggcggtg
     4261 atctcattac gaccatatac cgcaatcgga cgcgccagat tacccgcagc aatgctgtca
     4321 aaatggctac cgataatggc cagtggttga acaatcatct tgcgcgtcca ccacagcgca
     4381 ctgctgatgt agatcgccgc aacaataatc atgctgataa acaccagtgt cgaaatctga
     4441 tagttacgct ggctttgcgc actggcggct tccagcacat ggttgatctc cagctgccag
     4501 gcctcaaagt taacgtcaaa cgcattctgt gatccctgca ccggcgcagt gaggaaatcc
     4561 gaaagttgat tactttcaag ccaggtcgcc tgatgttcga gatcgttgtg ccagcgggca
     4621 aaacttttct ccgtctcttt ttgcaatgcc ctgacgtgct cgttgcccgc agtcatcgcc
     4681 ataaaacttt taaacagcgt ggtggattgc gtcagactgg cgcgcgccgt cgtcatcaac
     4741 gttttaatgt catccgccgg atagctaagt gccgtcagag tgcccgcttt gttcagcgcg
     4801 gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg ctgattgctc
     4861 tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga catgccgtta
     4921 ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt cgaaattcga
     4981 atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt aaatactcgt
     5041 caaaatgttt cccagtttgg atcttgttca gcaattcgca gtcgtggctg tgccggtggt
     5101 tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct ggctgccaga
     5161 cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc agcggcggcg
     5221 gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat ttccgaaacc
     5281 gccaatgcga cttgatcgat gccacggctc tgttcatccg atgccgatgc aatctcgccc
     5341 ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc cccggcgctt
     5401 tcgaccagca ccgaaccggt atcaacgcgt gcgacggagt cttcaatgag ggctttgatc
     5461 tcttttgccg cctgggcgct gcgactggca agattacgca cttcacccgc caccacggca
     5521 aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag cgcgaggata
     5581 ttagtctgga aggcaatacc gtcgataacg ctgataatgt cggcaatttt cttcgaacta
     5641 tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc gtgctgggcg
     5701 gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc gttttgcttc
     5761 actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga tgcctgctgt
     5821 tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc ggcatagatg
     5881 gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg catatgtgaa
     5941 acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt attcgctagg
     6001 ttaccaccgg cgatttcgcg aatgtgagca ataatttttg ccagcggagt aagcaacata
     6061 cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc gataaccgcc
     6121 agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc gcgatacagt
     6181 ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg cattccctgg
     6241 gttggctgag cgaaataagc tccagtattg ccataatcta gataatcaat cagttcagtt
     6301 aacgctgtgt aatagttttt atatttttca tcaatattac gactggtagc gaccatttca
     6361 ggtaacggtg ccatgctttt gaatttttta taatgcgtcg ctgcctgcgc caatgttttc
     6421 ctggcgctat cgagcaattc aactttggcg ttactttgtt gattggagga atccatcatc
     6481 atccgcaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc ccaggttgac
     6541 gtcagctcgc cctgctgttc ccgcaattga ttggaaacca caaagctctt ctggctatgg
     6601 tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa tacccccagc
     6661 accattacca acagcgtgac tacgcggata cggttaatca taaggcacct tcctgaaaac
     6721 aagttgatct cgttatcggc aagaaggggg gaaactttat tgctgatgcc acccgccgcg
     6781 aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcgg
     6841 gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc
     6901 agtttttcga tgttcaccag aatcaacata cggtcgccca gtgcgcccag tccagtgaga
     6961 tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc cgccgtcaat
     7021 gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg
     7081 acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag
     7141 tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg
     7201 ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag gatatcaata
     7261 ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc
     7321 agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg
     7381 tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg
     7441 ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac
     7501 tttcaaggtt tttaaccaca acctggtgtt gaccaattaa ttgatccacc agcaaggcgt
     7561 agcggcgacc gccactttgt aagatcacaa caattccctg ggtggcttcg gttttcgcgc
     7621 ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc cgcacttcca
     7681 gtacccgctc gccgccggcc agtggatgga gatcggcttc acggggttgc agtgattcca
     7741 taacagcatt cagcggcaga atgaaaactt catccccaac gcgtacggac atgccgtcga
     7801 ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtcccc tgcttcgact
     7861 ggatttcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc atgccgacgc
     7921 cgcgcccgga gacgtcggtg acctgctctg ccatggagaa gcctggtgca aatatcagca
     7981 tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag gccgcttttg
     8041 ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg caaatgttgc
     8101 cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct gcggcgagcc
     8161 gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg gtcagcgggt
     8221 cgataatgcg ttctatcagg cttttgtcga gttcagtaga actgcccacc agcgtcagtt
     8281 ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag cgactaaaaa
     8341 catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca cgggcgttac
     8401 gttgtaactg ccccatgctg gttatcaaat caccatgatt aaccgggtcc agttcgctgg
     8461 aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta attaattgat
     8521 caaccttttc taccgctaca cggatgctgg tggattcatt gctgcgcgtc gttttttccc
     8581 gctccacgcg gccggttggc gcttgttcgg ctgccagttt aagcactggt ggggtggata
     8641 tttttggcga gacttctact gtttcaaagg taatctgatc ggcttcaatc acaaaacaga
     8701 gtaccgctgt gatgtcatct tcggcgatgt cgcccggtaa tattgccgag agcgaatccg
     8761 cccctttcac cacgtcagtt aacgttgtca gatgtcccag ttcttcttcc agcaggtcga
     8821 cttccccggc cttcaggcgc gaaaggataa ttcgtcgcgg cgactgactg cgactctgct
     8881 catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat ggcgtttcgc
     8941 ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag ctggcggcat
     9001 ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc ttcgtttcca
     9061 aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct tcatcgagca
     9121 ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt cctgcccctc
     9181 ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct ggcgcttccg
     9241 gctgcaaaac cagcagatgc tgctccatgt cagccaacag ttcgtccgct tcatcaaaaa
     9301 atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc ggctgatggc
     9361 attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct tactggctca
     9421 ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt cagtaccagc
     9481 aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc
     9541 gccatgccga cgacacgtaa cactttgccg ctatccaacc ctccgaccat cagttcgcgg
     9601 cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tatatccttt ctcaccgctg
     9661 gcgtagggga aatcatcggt atgacctgaa aggctaatac ggttgggaat accgttcagt
     9721 acaggcgcaa tgacacgcag aatgtcgcgc atatagggtt cgacatcggc actgccggtt
     9781 ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc ctggaccaga
     9841 tcgattttga gatggggacg taacgcccgc agtttcggat cggactctat caactggtcg
     9901 agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcag atcttcgatg
     9961 ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccacc gccgggaatt
    10021 gggctttcac tattagaaat gcgatcgccg cccgtaaccg cagtcgccag tggagtccgg
    10081 aagtactccg caatctgaat cagctctttt gggctggaga tggagatcag ccacatcacc
    10141 agaaaaaagg ccatcatcgc agtcataaag tcggcataag caatcttcca cgatccatgt
    10201 gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg cgcttgattc
    10261 ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc acatgctctt
    10321 ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca aactcaacgg
    10381 cgataggcgg tgcgtaaccg ttcagattag aaagcagagt gactttgacg cactgcatca
    10441 ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa ataaatccgt
    10501 aagccaataa aatgccgagg aaagtcccca ccatcgcatg tgcgataagc gcacccagct
    10561 cggcggcagg acgatcggct gaacctaacg cgtgaacgcc ccccattaca gccgcgacaa
    10621 taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg acttctgcct
    10681 cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag gtgttcatat
    10741 gaccgctgat aatcagacgc agataatcga cgataaaatc aagcatgacg ctatccgcga
    10801 ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata tcacgttcca
    10861 gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga gccagcagat
    10921 ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc gccttcagcg
    10981 tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct gcaccggcaa
    11041 taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc atcaaataac
    11101 cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg acatcatcct
    11161 tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga ttggggcaaa
    11221 aaaagcagcg gtacgtcgtt accgctactg gaatgttgcg cctcaccgta tcagttaaac
    11281 agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat tctgggaaag
    11341 tttacgtctt tttactgccc gggatggcgg ttgacataag ctgcaggcaa agctgccaac
    11401 aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg aaagttgcag
    11461 taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc
    11521 ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca ccgcatcgac
    11581 gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg
    11641 aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg
    11701 tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta actgtgtttc
    11761 gctttccagc atctgcaaac gagcgcccag ggtaatcaat tccattgcca gctgaatatc
    11821 ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt gcgcagcgct
    11881 tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt
    11941 tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg gtggctgtca
    12001 aaacggaagt gacaaaccag ttgattggtt tctgccagct taaccatttg cggaagagtc
    12061 agtgccgcta acgttgtcgc catttcttca tttatgccga gacgaaacat agcggacgct
    12121 ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg acaagttgat gtcataaatg
    12181 tgtttcagca actcggaggt atgcattatt cccacccaga ataaccaact tt
//
LOCUS       DAJTVJ010000124        12216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000124 DAJTVJ010000000
VERSION     DAJTVJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.124"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="OO448_003382"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="OO448_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6338510.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="OO448_003383"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="OO448_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6338511.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="OO448_003384"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="OO448_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6338512.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="OO448_003385"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="OO448_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR6338513.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="OO448_003386"
     CDS             complement(2270..2761)
                     /locus_tag="OO448_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6338514.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="OO448_003387"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="OO448_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6338515.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="OO448_003388"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="OO448_003388"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6338516.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="OO448_003389"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="OO448_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR6338517.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="OO448_003390"
     tRNA            5405..5492
                     /locus_tag="OO448_003390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="OO448_003391"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="OO448_003391"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="OO448_003392"
     CDS             complement(5764..6450)
                     /locus_tag="OO448_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6338518.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="OO448_003393"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="OO448_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6338519.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="OO448_003394"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="OO448_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6338520.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="OO448_003395"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="OO448_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6338521.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /locus_tag="OO448_003396"
     CDS             complement(10902..11732)
                     /locus_tag="OO448_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6338522.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3225 a         3167 c         2823 g         3001 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt caacccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgtaact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaag
//
LOCUS       DAJTVJ010000125        12037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000125 DAJTVJ010000000
VERSION     DAJTVJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.125"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            9..323
                     /locus_tag="OO448_003397"
     CDS             9..323
                     /locus_tag="OO448_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6338523.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="OO448_003398"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="OO448_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="OO448_003399"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="OO448_003399"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6338524.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLSMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="OO448_003400"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="OO448_003400"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6338525.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="OO448_003401"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="OO448_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6338526.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="OO448_003402"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="OO448_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6338527.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="OO448_003403"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="OO448_003403"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6338528.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="OO448_003404"
     CDS             8424..8927
                     /locus_tag="OO448_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6338529.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="OO448_003405"
     CDS             9004..9687
                     /locus_tag="OO448_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6338530.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="OO448_003406"
     CDS             9766..10752
                     /locus_tag="OO448_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6338531.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="OO448_003407"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="OO448_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6338532.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2861 a         3094 c         3225 g         2857 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gtggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtcta tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAJTVJ010000126        11877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000126 DAJTVJ010000000
VERSION     DAJTVJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.126"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1760
                     /locus_tag="OO448_003408"
     CDS             <1..1760
                     /locus_tag="OO448_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6338533.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="OO448_003409"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="OO448_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6338534.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="OO448_003410"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="OO448_003410"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6338535.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="OO448_003411"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="OO448_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6338536.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="OO448_003412"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="OO448_003412"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR6338537.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8753
                     /locus_tag="OO448_003413"
     CDS             7437..8753
                     /locus_tag="OO448_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6338538.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSA
                     LYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKF
                     LIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
                     MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            8774..9436
                     /gene="csiR"
                     /locus_tag="OO448_003414"
     CDS             8774..9436
                     /gene="csiR"
                     /locus_tag="OO448_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6338539.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="OO448_003415"
     CDS             complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="OO448_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6338540.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9970..10128)
                     /locus_tag="OO448_003416"
     CDS             complement(9970..10128)
                     /locus_tag="OO448_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6338541.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10311..10610
                     /locus_tag="OO448_003417"
     CDS             10311..10610
                     /locus_tag="OO448_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6338542.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10620..11144
                     /gene="ygaP"
                     /locus_tag="OO448_003418"
     CDS             10620..11144
                     /gene="ygaP"
                     /locus_tag="OO448_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6338543.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="OO448_003419"
     CDS             complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="OO448_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6338544.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2965 a         2928 c         3062 g         2922 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggccctgtat accgcctcaa
     8281 ggatgctcta ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc
     8341 gcagtaaaac cccgtacgtg gcggtgttac tctccaccgg cgcggcattc ttaacggtgg
     8401 tggtgaacta ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc tccggcgcta
     8461 tcgccctgct ggtttattta gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc
     8521 gagcggaagg aagcgaaatt cgcttgcgaa tgtggcttta tccctggctc acctggctgg
     8581 tcatcggctt tattaccttt gtgttggtag tgatgctatt ccgtccggcg caacagttag
     8641 aagtgatctc caccggctta ttagcgatag ggattatctg taccgtgcca attatggctc
     8701 gctggaaaaa gctggtattg tggcaaaaaa cacccgttca taatacgcgc tgataatgat
     8761 caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga agaacgatat
     8821 tattcgcggt aattttcaac cggatgaaaa attacggatg agtttgctga catcgcgtta
     8881 tgcacttggc gttgggccgt tgcgggaagc actttctcaa ctggtggcgg aacggctggt
     8941 cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg agctgctcga
     9001 tattttcgat gcccgcgcca atatggaagc gatgttagtg agtctggcga ttgcccgcgg
     9061 tggcgatgag tgggaggcag acgttctcgc aaaagcgcat ctgctcagta agctggaagc
     9121 ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg cgtttcatac
     9181 agcaattgtg gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac ggttgtttga
     9241 tctggcggcg cgctatcgat ttatctggct gcggcgaacg gtgctttcgg tagaaatgct
     9301 ggaggataaa cacgatcagc accagaccct gactgcggcg gtactggcgc gagataccgc
     9361 gcgcgccagt gagttaatgc gccagcattt actgacgcca attcccatta tccagcaggc
     9421 gatggctggc aattaactac tcttccggaa tacgcaacac ttgccccgga taaattttat
     9481 ccgggctttt tagcatcggt ttattcgctt cgaagatttt attgtacaga ttagcgttac
     9541 cgtagacctg tttggaaatg gcactcagag tgtcgccaga cttaacggta taaaactggc
     9601 tggcagtggc tggtgtcgcc gttttcacct gatcatcaac actggcaata ccggaaatat
     9661 tccccaccgc aacaaggatt ttctccttcg cctcctgact caggccgtca ccagtgaccg
     9721 tcgctttgcc gtcggcaatt tgaatgttca ctttatcagc atcaggtata ccggttttgc
     9781 tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct gtaaccgcgt
     9841 cccagagttt ttctccggca tctttcacaa aattgaacag acccatagct acctcattcg
     9901 ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg
     9961 agcacaggtt taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg ggatataccc
    10021 aagcagtgtc agcagaatat taataatgaa cgcccaaccg aaccctttac cgagcagcac
    10081 gccgagcggc ggtagaatga tggtgatgac gattctccag aaacccatat gtactcccta
    10141 taagaaaatt actcattgtt taaaaagaga ttttatctct taagcgtagt aattttagcg
    10201 gagcctgcca gtttcagctt acgcttttac tctcatttac gctgctgagg ctggtatatg
    10261 ttgcattttt agtttagagc ttacttaatt tagaaaatac ttaaataaat atgactgaac
    10321 tcgcgcaatt acaggccagt gccgaacagg cagcggcctt attgaaagca atgagccatc
    10381 ctaaacgatt gctgattctg tgcatgctta gcggttcccc cggcaccagc gcgggagagc
    10441 tgacgcgcat taccggactg agtgcctctg cgacatcaca gcatctcgct cgtatgcggg
    10501 acgaagggct tatcgacagc caacgggatg cccaacgcat tctatattcc attaaaaatg
    10561 aggcggtaaa tgccattatc gccaccctga aaaatgtcta ttgtccgtaa ggagtcacta
    10621 tggctttgac aaccatttcg ccgcatgatg cacaagaatt aatcgcacgc ggcgcaaagt
    10681 taatcgatat tcgtgacgct gatgaatatc ttcgtgaaca tattcctgaa gcagatctgg
    10741 ctccattatc cgtgctggaa cagtcaggtc ttccacctaa attacgtcgc gagcaaatta
    10801 tattccactg ccaggcaggt aaacgcacca gtaataacgc cgataaatta gcagcgattg
    10861 ccgcccccgc agaaatattt ttactcgaag atgggattga tggctggaaa agagctggat
    10921 tgccagtagc ggtaaataaa tctcaaccct tgccgttaat gcgccaggtg cagatcgctg
    10981 cgggtggttt aatattaatc ggcgttgtac tgggttatac cgtaaatagc ggtttcttct
    11041 tattaagtgg ctttgttggt gccgggttac tgtttgcagg aatcagcggt ttttgtggaa
    11101 tggcaaggtt gttagataag atgccgtgga accaacgagc ttgagaagcg acgccggacg
    11161 cgccctagca gcgacatccg gcctcagtaa ttagatcagg aaatcgtcga gagatttacc
    11221 ttctgccagc gcctgagcaa ttggcttcgg tgtacggccc tgaccggtcc aggttttagt
    11281 ttcaccgtta acatcggtga atttatattt cgccggacgc ggctggcgtt ttttaccagc
    11341 gcgtggtgca gcagcagagc tattacccaa taactcttcc gggttaattc cgtcagcctt
    11401 catcagctcc agccaggtgc taattttttc ctgacgctca gccagttcac gctgctgctg
    11461 ttcttcttct tcacgtcttt ctttagtgac aaccctgaat ttttcgagca tttcttcaag
    11521 aacgtcaatg gagaattcgc gagccatcgc acggagggtg cgaatgttat ttaaactttg
    11581 taacattacg gacataataa agaaaacctt ttaacgccaa aacaaaaaag tatttcgtcg
    11641 cgtagtgtaa gcgatcactt attatatttc cacaaaattt aatctattta cagtcttgcg
    11701 cgagattatt tcagctgtta ttttctgaaa ctctcagaat aatttcaaga ttatatatta
    11761 gccattttga tgagtaattt agattattgg tgaacagaac ggcgctctct gctcgctaat
    11821 tccgcaagcc tgacaaaacc gaagtgaata aaaatgcaat ataacagaat aaaagaa
//
LOCUS       DAJTVJ010000127        11780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000127 DAJTVJ010000000
VERSION     DAJTVJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.127"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..612)
                     /locus_tag="OO448_003420"
     CDS             complement(<1..612)
                     /locus_tag="OO448_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6338545.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRF"
     gene            complement(735..2381)
                     /gene="fumB"
                     /locus_tag="OO448_003421"
     CDS             complement(735..2381)
                     /gene="fumB"
                     /locus_tag="OO448_003421"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6338546.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2459..3799)
                     /gene="dcuB"
                     /locus_tag="OO448_003422"
     CDS             complement(2459..3799)
                     /gene="dcuB"
                     /locus_tag="OO448_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6338547.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4370..5089)
                     /gene="dcuR"
                     /locus_tag="OO448_003423"
     CDS             complement(4370..5089)
                     /gene="dcuR"
                     /locus_tag="OO448_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6338548.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5086..6717)
                     /gene="dcuS"
                     /locus_tag="OO448_003424"
     CDS             complement(5086..6717)
                     /gene="dcuS"
                     /locus_tag="OO448_003424"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6338549.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6898..7128
                     /locus_tag="OO448_003425"
     CDS             6898..7128
                     /locus_tag="OO448_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR6338550.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7140..7412
                     /locus_tag="OO448_003426"
     CDS             7140..7412
                     /locus_tag="OO448_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6338551.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7639..7935
                     /gene="ghoS"
                     /locus_tag="OO448_003427"
     CDS             7639..7935
                     /gene="ghoS"
                     /locus_tag="OO448_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6338552.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7963..8136
                     /gene="ghoT"
                     /locus_tag="OO448_003428"
     CDS             7963..8136
                     /gene="ghoT"
                     /locus_tag="OO448_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCR6338553.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8255..9772)
                     /gene="lysS"
                     /locus_tag="OO448_003429"
     CDS             complement(8255..9772)
                     /gene="lysS"
                     /locus_tag="OO448_003429"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6338554.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10009..11466)
                     /gene="dtpC"
                     /locus_tag="OO448_003430"
     CDS             complement(10009..11466)
                     /gene="dtpC"
                     /locus_tag="OO448_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCR6338555.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11525..>11780)
                     /locus_tag="OO448_003431"
     CDS             complement(11525..>11780)
                     /locus_tag="OO448_003431"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6338556.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3045 a         2856 c         2892 g         2987 t
ORIGIN      
        1 aaagcgccgt agctggcggc aatgaaagcg agcgaggaat atcaccgtcg ttaatgcgcc
       61 aagcagcgcg cagaacatat cggattgcgt gtcccactgg tcgccctggg tgccgagaaa
      121 atcatccgct ccctggccca ttgccagcgc cgcccaccac tcgattaatt catacatcgc
      181 gcttatcgcc agcgccacgc agcagactag gaacgccacc attttacgtc cgcgcacgta
      241 catcccgcgc acgagaattt ctcgtgccac cagtgcaggc accagcccct ggaaaaaatg
      301 ccccagcttg tcatacggat tacggctcaa ccccagccat tcctgcacct caaaaccgat
      361 ggggactttc gcgtaggtgt attgcccgcc gaccatcagg atgatggcgt gaaagaaaat
      421 gagcgtatag agcagcggcg ttaacggata acgtttagcg gtggcaagca gtagcggcac
      481 gacaataatc accggtgtca cttccatcag ccaggtgagt ttgtcatggg ccgaaatgcc
      541 ggtataaatc aggattaacg ttagcgccag tgcgccggtg ttaagaatta acggtttgag
      601 tgtgcgggtc atggtgagtt cgcaagtcag ggaaaatact gactattcac cagcaagcat
      661 gaaattacaa tcgcaaaaga ggtggcccag ggggatcacc tggcagcatg ctgccaggcg
      721 ctgggccgaa gaggttactt agtgcagttc gcgcactgtt tgttgacgat ttgctggaag
      781 aagtcgttac ctttgtcatc gaccaggata aacgccggga aatcttctac ttcgattttc
      841 cagatagctt ccatacccag ctccggataa gcgacgcatt ccagatgctt gatgctctgc
      901 tgcgccagta ccgccgccgg accgccgatg ctaccgaggt agaagccgcc gtgtttatga
      961 cacgcgtcgg taacctgctg gctgcggttg cctttcgcca gcatgatcat gctgccgccg
     1021 tgggattgca gcagatccac ataggagtcc atacgacctg cggtggttgg gccaagtgaa
     1081 cctgatggat aaccggcagg agttttcgcc ggacccgcgt agtagatcgg gtgatctttg
     1141 atgtactgcg gaagttcttt accggcgtca atcagctctt tcagcttggc gtgtgcaata
     1201 tctcgaccca caataatggt gccagtgagc gacaaacgag tggaaaccgg atattgcgaa
     1261 agctgggcga ggatctcttt catcgggcgg ttaaggtcga ccttcaccgc ttcgccttca
     1321 ccggcctgac gcagttcttg tggaatgtac tgacctggat tgtgttccag tttttcgatc
     1381 cagatacctt cgcggttgat tttcgcttta atgttacggt cagcggagca ggagacgccc
     1441 atgcctaccg ggcaggatgc gccgtgacgt ggcagacgga taacgcgaat gtcgtgcgcg
     1501 aagtatttac cgccaaactg cgcgccaaga ccgagtttct gggcctcttc cagcagttcc
     1561 tgttccagct ggacatcgcg gaacgcctga ccatgttcgt tcccttccgt cggcagttca
     1621 tcgtaatagt gagcgcttgc taatttgacg gttttcaggt tggtttccgc agacgtaccg
     1681 ccaatcacaa acgcgatatg gtacggcggg caggctgcag taccgagggt acgcattttc
     1741 tcgacgagga agtttttcag tttgccggga gtcagcaggg ctttggtttc ctggtagaga
     1801 tacgttttgt tggcagagcc gccgcctttc gcaacgcaaa ggaatttgta ctcatcgcca
     1861 tctaccgcgt acaggtcgat ttgcgcaggc aggttagtgc cggtgttgac ctctttgtac
     1921 atgtccagcg ccgcgttttg cgagtagcgc aggttatctt cgatataggt gttatagacg
     1981 cctttcgaca gcgcttcttc atcaccgcca ccggtccaca cgcgctggcc ttttttaccg
     2041 acgatgatcg cggtgccggt atcctggcag gtcggcagca cgcctttggc ggcgatttcg
     2101 gagtttctta agaattgcag tgccacgtac ttgtcgtttt cgctggcttc tggatcgtga
     2161 agaatagccg caacctgttt ctggtgtgcc ggacggagca tgaaagaagc gtcgtgaaag
     2221 gcttgctgcg ccagcagggt cagggcttct ggttccactt tcaggatggt ttcgccgtcg
     2281 aagtcggcaa cgctaacgta atcggaagtg agtagatagt attcggtatt gtctttcccc
     2341 atcgggaaag gtgcctggta gataaagggt ttgtttgaca tagcttccag cctgtaactc
     2401 tgtatttgtt attcgaaaat ggcgtgccgc gtagtcacgg gcggcacgca aagtgcattt
     2461 ataagaaccc gtacatcgcg gcgaagatcc agccgaagac gcacgatacg ctcacaccaa
     2521 tcaaccccgg cagaataaag ctgtggttga tgacgaagcg accgatgtgg gtggtgccgg
     2581 aacggtcaaa ctgaatcgct gccagatcgc tcggataagt cggcaggatg taataaccgt
     2641 agcaagccgg tgctgaagcc acgatgtatg ccggatcaac gccgatcgcc agcgcgaccg
     2701 gaacaatcgc cgccagcgcc gcagcctgag agtttacaaa cttggaaacc agcagcagaa
     2761 caatggcata ggcccacgga tactctttca ccatttcacc cagtacgccc tgaatttcag
     2821 acatatgcgc accgaacatg gtttctgcca tccatgcgat accgtacacc gccacgatgg
     2881 cgatcatacc ggaacggaag acttcgtttt ttgagataga cgcgggattg gttttggtca
     2941 ggataataat cagcgccccg gtcagcagca taaacatctg aataaccagt accatcgaca
     3001 gcggtttgcc gccgaaggat ggacgcaggt ccgaatcagc accaagaagg gcgactacag
     3061 cgattgcccc gaggaaaatc cacattgcca gccagttgct tttcggcagt tttttgtcca
     3121 gcagcgtcgc ggtatcaccg taaacatact cacggttttc cggtacggag atgaatttct
     3181 ggaactcttc gtctttatcc agatctttac cacggaacca gctgaagata ccgatcgcca
     3241 ggataccgat taacgtcgat ggaatggtga ttgccagcag gtcgaggaac tcaagatggc
     3301 gaccatcaaa ggtgacatta cccagcattg caaccagaga cacgaccgca accgacaccg
     3361 gactggcgat gatccccatc tgtgcaccga tagaacttgc cgccatcgga cgttccggac
     3421 ggatgttgtt cttaatggcg acgtcgtaga tgatcggcag aatggtgtaa accacatgac
     3481 ccgtaccgca aagaatggtc agcgtacagg tcacaaacgg cgcgacaatt gagacatatt
     3541 tcgggttgcg gcgcagcagc ttctcggcaa tttgcagcat gacatcaaga ccgcccgaag
     3601 cttgcaaggt cgccgatgcc gccaccaccg caatgataac cagcatgaca tcaaccggtg
     3661 gtttacctgg ctgaaggtgg aagacgaaga ccagaatgac cagaccgata ccgcctaata
     3721 aacccagcgc gataccaccc tttctggcac cataaaacag acatatcagt attatgataa
     3781 gttggatagt aaataacatg tgtgaaccct cgcgataatc ctatttaaat ttttgctgaa
     3841 tagatcacag tcacgttctg ttttgtatga actgttttca gagcctgact aaatatccgt
     3901 ttggtcgtta ccggctttag caaatacctc acagtgaata ttggctgatt aaagcagcgg
     3961 caaatttatg cactgtgata agcggctttt tcagagagaa agactctctg gcgatgggtt
     4021 tatagtttgt actgcgcaga tgttaatgtt ttgtttgctg ttatcttatt gatatttatc
     4081 ggtctatttt ttgttctcag ggcagatgtc attaggttta tagattaatc tgatctaccc
     4141 atttgtgggt aaaaatacac ataatgcggg tgacataata gttaattaac ttttgttagc
     4201 gttttgaaat taaaaacacc gttcacctga agagatatta atttttagcg atgatgcagg
     4261 gataattata tttgatctgg cacaagtttt actgatgaag gatgtaactt gtgccagggg
     4321 tattttgcat tacggtaata attattactt acagataacc agcgatccgt tattggcaat
     4381 attgtttcag tagtgagtag tgttctgcct gaatacggta acggtaaact ggacgccccg
     4441 tgacgccata atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt
     4501 tacggcagga aacacgcgaa atgttaacct cgttggctag ctcgtcggtt gaaaattcat
     4561 agtcctgatg cgcgtcaatc cactggcaca gtgtgcgtaa cgtctgcggc gttaagcctt
     4621 ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct
     4681 cggcctggtc ataatactga tgtttttcca gcgccatttt cttttgccgc cagtcggtga
     4741 gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat
     4801 gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt
     4861 tgcaacgcgc gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga
     4921 tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct
     4981 ccagcgtcga ggctgttcca cagcattgaa agcctgggat ttgtgctacg tatcggcgat
     5041 tcagctccgc gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct
     5101 ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac
     5161 ggcgatgctg ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg
     5221 ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat
     5281 ccccggtcca tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac
     5341 gctaatttcg ccaccgggtt ccggccctaa tgcttccagc gcgttttcta tcagatttcc
     5401 caatgtggta atcagcgtcg cgacctggtc ctcactgccg ctgtctggca gctggctttc
     5461 actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcga ttaatcttgc tgattaaaaa
     5521 accagcgata accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt
     5581 attggctgtt tttagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc
     5641 gagaatcacg tgcaatttat tcataaattc gtgggatcgt tcacgaagtg cgtcggcata
     5701 gttgaccaga ccgtcgagtc gctgcatcag tttacgtact tcggttttgt ccctgaaggt
     5761 tgaaatggca ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag
     5821 ccggtcttta atcgtaatct cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga
     5881 gacatctacc acctgtgacc atgagtggct gagcgtcgac agtttctcat cgtcctgcga
     5941 cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc
     6001 gtccacggca acgacgcctt ctttgataga ctgcaacatg gcctggcgtt gctcaaacag
     6061 cgtggagatt tcgtagggtt ccaggccgaa aaggattttt ttcagtacct taaccagaat
     6121 gcaggtgcca atcagtccga ccagcatgcc aaataatacc gaccagataa tgctccagcg
     6181 actgtcattg atttgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat
     6241 ttgtttatga ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc
     6301 gcgattgata gcgacatttt ctttgccatt cagcgcttta aggatgtcat cacctttaaa
     6361 tggctgacca atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac
     6421 gacaataaac agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggatgccgct
     6481 ctcctgcggt tttttctgca agccctgacg gatttccggc gagttggcga gggtacgcgc
     6541 cactgccagt gccttgttgg ctagcccgtc tcgcgtcata tcactgattt gcgagaagta
     6601 aatcagatgc accaccaata gcaccgagaa cagtaccgca ctgaccatta agatcactgt
     6661 ggtactcaat ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag
     6721 cttccttgtg tgacaaattt cttaaacatt atctctgatg aggtgggtaa ttcaaaggga
     6781 gtaagaatga ttggctatat aggggaagag attctggcaa cggaaactgc cagtgctgta
     6841 tgaagattcc agggctatgc ttatagcgat aatcatactg atgagagagg gaaagtcatg
     6901 gatcaggcgc tactggacga gggttatcgc tgttataccg gcgaaaagat cgatgtctat
     6961 ttcaacactg cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt
     7021 aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg acgtcgccac tgtggttaaa
     7081 gtgattgata cgtgcccgag cggcgcgctg aaataccgtc ataaataagc gagggtaaaa
     7141 tggaaatacg cgaaggccac aataaatttt acattaatga cgaacaaggc aagcaaatcg
     7201 ctgaaattgt ctttgtgccg actggagaga atttagcgat tatcgaacat accgatgtcg
     7261 atgaaagcct gaaagggcag gggattggta aacagctggt tgcaaaagtc gtggaaaaaa
     7321 tgcgtcggga aaaacgaaaa attatcccac tatgcccatt tgcgaaacac gaatttgata
     7381 aaacgcggga gtatgatgat attcgcagtt gatgggagag tacagagtca cgatattttt
     7441 catgctctcc gcggagtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat
     7501 gggcagagag cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata
     7561 tctcctatat gagaatcatc aatcggggtt atcaagtttt gcgtccccag agcattcaat
     7621 attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt tgtttctttt
     7681 gattatccat catcttattc ctcagtgttc ttaaggttaa gatcattgat gtatgatatg
     7741 aatttctctt ctatcgtggc tgatgaatat gggataccac gacaattgaa tgaaaattcc
     7801 ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag gcttaaatgc
     7861 ttagacttac cggatattga ttttgacctc aacattatga cagttgatga ctatttccgt
     7921 cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact attctctaaa
     7981 atattaattt tttatgtgat tggtgtgaac atatcctttg tcattatctg gtttatctca
     8041 catgagaaaa cacatattcg tttacttagt gcattcctgg tcggaataac ctggccaatg
     8101 agtctgcctg tggcattact tttttctctc ttttaggagc tgtagttgag tggacccgct
     8161 ggttatcgtg aaacgaagcg tctctcctca aaactggatt tgcggaacga cgaacgaagc
     8221 agtcgcctga ctgcttcgtt cattaaagtg aaatttattt ctgtgggcgc atcgccggga
     8281 agagaataac gtcgcgaata gtatgactgt ttgtaaacag cataatcatt cggtcgatac
     8341 caatacccag accagcggtt ggcggcagac catattccag cgcagttacg taatcttcgt
     8401 catagaacat ggcttcgtcg tcacctgcag ctttagcgtt aacctgttcc tggaaacgtt
     8461 cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt accgatttca cgaccaccga
     8521 tgaagaattc aaaacggtcg gtgatttccg ggttaacatc attacggcgc gccagcggtg
     8581 acacttctgc cggatattcc gtaataaagg ttggctgaat cagatgtgct tctgccactt
     8641 catcaaagat ctccgtgata atacgtccca gcccccagct tttctctacc gtaattccga
     8701 tagattcagc taatgcttta gcagcatcaa aattatccag gtcggccata tcggtttctg
     8761 gacgatattt tttgattgct tcgcgcatgg tgagtttttc aaacggtttg ccgaaatcaa
     8821 acacatgctc gccataagtg actttagtgg tacccagaac ctcttgtgcc agggtgcgga
     8881 acagtgactc tgtcagttca atcaaatcgt ggtaatccgc atacgccatg tagagttcca
     8941 tcattgtgaa ctcaggatta tggcgaacag aaataccttc attacggaag ttacggttga
     9001 tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt cagatacagc tccggcgcga
     9061 tacgcagata catatctaaa tccagagcat tatgatgggt aataaacggg cgagcagatg
     9121 ccccacctgg aattacctgc atcatcgggg tttctacttc cataaagccg cgcgcgacca
     9181 tgaactgacg gatagcggcc agaatttttg aacggacaac aaacgtttga cgggatttat
     9241 cgttagcgat gaggtccaga taacgttgac gataacggac ttcctgatcc tgcagaccat
     9301 ggaatttatc tggtaaagga cgtagtgctt tagtcagcag gcgcaactca gtacagtgaa
     9361 tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg ggcaccgata atgtcaccca
     9421 gatcccattt tttaaactga tcgttataaa caccttctgg caggctatct cttgcaacgt
     9481 acagttgaat acggccaccg acatcctgca acgttacaaa ggaggctttc cccatgatac
     9541 gacgggtcat cattcggcca gcaaccgaga cttcaatgtt taaggattcc agttcctggt
     9601 tattcttcgc atcaaactct tcgtgcaact ggtcagaggt atggtcgcgg cgaaaatcat
     9661 tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt ttcgcggcga tttctcagtt
     9721 catcgttaaa atcaatagcc tcattggctc cccgtgtttc ttgttcagac attttggttc
     9781 ctctaaatcc agctttcaat ttagcgtaga taaagagaca gatcggtaca ctgtattatc
     9841 tgcctcgact attaataact cagtttatct tatcgaaact aacgcttacc tcaagttgat
     9901 atcactaata aataaccatc aaaatggttt aaatgactta ttaatctcga tttactagtg
     9961 tgattaactg aaagttaatt tattcataac caattgtttt tacgacagtt aatcgttgct
    10021 ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg gtagcaaagg ccaatacgac
    10081 gatgatcgcg acacaagcca acgtccactc tcccatctga gaaaagaatc gctggtaagc
    10141 tgcaattacc ataccgctaa tttgcgactc tgtcgtctgc tgtgccacga cgcctgccag
    10201 ccagttggcg accgcgcctg ttgccagcat ataaataccg gttaatacgc cagacatttt
    10261 cagacgcgtg atttgcgcaa tcgccaccgg gtcaataaag agttctgcaa agcccattag
    10321 cgccaacccg gatatcatca cgcccattga cgcttgaccg tcagccgctg catgtcgggc
    10381 atcaaatgcc aacaacataa agccacaagc catcagcagt aagccaaagg caaacttcag
    10441 ccagacgcgc aatgttgagt tgccgcggct ttctggcgac gccagccagg ccagtacaac
    10501 cccagcgagc atcactgcaa ttgcattcac cgactggaat agtgctgtag gtacttcaat
    10561 attgaatgcc tgacgattca caaagcgatc gataaacaag ctgatggtac tgccgccctg
    10621 ttgtgccagt acccagaaca acgtcccgac aaacatcaac aatacaattt gccaaagagc
    10681 acggcggtgt tcggggaatt tgatcatcat gcgggcaatg atttgtgcgg caatgaggca
    10741 aacgatcgcc agcaaatatc cggaccagtc gttctccagc agcagggtaa aaaatactgg
    10801 ggctaaacag agcatcacca ctaaccagct ccatactggt aaggcaaatt tgacgctagt
    10861 gagcgctttt ttatccatac tacgcgtgga ctggaaatga cgatgaccgc ttaagaaaat
    10921 caacaaaccg ataaacatgc cgccacccgc aagggcaaag ccaacatgcc atccatacca
    10981 ctgagcagcc aggccgcagg cgattggggc ggcgatagaa ccgatattgc ccgcagcata
    11041 cagcagcgaa aaaccgccat cacgtcgatg atcgttctcg tcgtagagct cgccaagcaa
    11101 acagctgatg tttgatttga ataaaccgta gccacaaata atgattgcca gcgccagata
    11161 caggctaaag gttgaatttg tatcaatgcc cagcaccaca tggccaaggg tcattaacag
    11221 cgcgccggca atcactgcag tgcggttgcc gagcaggcgg tcggcaagcc agccgccgag
    11281 aataggggta acgtaaacca gagaagcata tgcgctgaac aggctgatgg catggttatc
    11341 atcaaaacca agctgatggg tgagatagag aatgagtaag gcacgcatgc cgtaaaaact
    11401 gaagtactcc cagatttgga tcgccacgat atagtatatc gcgcgcggct gtgagggtgt
    11461 tttcatgtgt tctccttatg agcaaaaaag ggaagtggca agccacttcc cttgtacgag
    11521 ctaattattt tttgctttct tctttcaata ccttaacggt atagcggcca tcagcctgac
    11581 ggtatgcacc gtgaatatcg gtttcaaatc ccggatagtg agtgccgatt tcacacagca
    11641 tctgcaggaa ctccagaacc ggacggcttt cttcggtgat catttcaccc ggcattacca
    11701 gaggaactcc cggcggatac ggaaggatca tattggcgtt aatacgacct accatttcgt
    11761 cgaggtaaac ttcttcggtc
//
LOCUS       DAJTVJ010000128        11729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000128 DAJTVJ010000000
VERSION     DAJTVJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.128"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            95..1135
                     /locus_tag="OO448_003432"
     CDS             95..1135
                     /locus_tag="OO448_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6338557.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1167..2360)
                     /gene="lplT"
                     /locus_tag="OO448_003433"
     CDS             complement(1167..2360)
                     /gene="lplT"
                     /locus_tag="OO448_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6338558.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2353..4512)
                     /gene="aas"
                     /locus_tag="OO448_003434"
     CDS             complement(2353..4512)
                     /gene="aas"
                     /locus_tag="OO448_003434"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6338559.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5098..6129
                     /gene="galR"
                     /locus_tag="OO448_003435"
     CDS             5098..6129
                     /gene="galR"
                     /locus_tag="OO448_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6338560.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6136..7398)
                     /gene="lysA"
                     /locus_tag="OO448_003436"
     CDS             complement(6136..7398)
                     /gene="lysA"
                     /locus_tag="OO448_003436"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6338561.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7520..8455
                     /gene="lysR"
                     /locus_tag="OO448_003437"
     CDS             7520..8455
                     /gene="lysR"
                     /locus_tag="OO448_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6338562.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8442..9089)
                     /locus_tag="OO448_003438"
                     /pseudo
     CDS             complement(8442..9089)
                     /locus_tag="OO448_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9220..10638)
                     /gene="araE"
                     /locus_tag="OO448_003439"
     CDS             complement(9220..10638)
                     /gene="araE"
                     /locus_tag="OO448_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6338563.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10953..11714)
                     /gene="kduD"
                     /locus_tag="OO448_003440"
     CDS             complement(10953..11714)
                     /gene="kduD"
                     /locus_tag="OO448_003440"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6338564.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
BASE COUNT         3014 a         3239 c         2821 g         2655 t
ORIGIN      
        1 catccggctg gccttaagat ttttctcttt cccttttccc ttccctctgc catttttata
       61 ttccttatgt cgtgattata aaaaggaaac ggctatgcaa tatcaccgta taccccacag
      121 ttcgctggaa gtcagcacgc tggggcttgg cacgatgacg tttggtgaac agaacagcga
      181 agccgacgcc cacgcacaac tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt
      241 tgccgaaatg tacccagtac ctccgcgccc cgaaacgcaa gggttaaccg aaacctacgt
      301 cggcaactgg ctggcgaaac atggcagccg cgaaaagtta attatcgcct ccaaagtgag
      361 cggaccgtcg cgcaataatg acaagggcat ccgcccggat caggcgctgg atcggaagaa
      421 tatccgcgaa gcgctgcatg acagcctcaa gcgcctacac actgattacc tcgatcttta
      481 tcaggtgcac tggccgcagc gcccgaccaa ctgcttcggc aaactcggtt atagctggac
      541 ggattctgcg cctgcggttt cgctgctgga tacgctggac gcactggcag agtaccaacg
      601 cgcgggaaaa attcgttata tcggcgtgtc gaacgaaact gcatttggcg taatgcgcta
      661 cctgcatctg gcggacaaac acgatctgcc gcgtattgtc accattcaga acccttacag
      721 tctgttaaac cgcagttttg aagtaggtct ggcagaagta agccagtatg aaggggtcga
      781 actgctggcc tattcgtgcc taggtttcgg cacgctgacc gggaaatatc tcaatggtgc
      841 aaaacccgct ggcgcacgta atacgctctt tagtcggttc acccgctata gcggtgagca
      901 aacgcaaaaa gccgtcgcgg cgtatgttga tatcgccaga cgtcatggcc tagaccctgc
      961 tcagatggcg ctcgcgtttg tacgccgtca accgtttgtt gccagcactc tgctgggcgc
     1021 aaccacgatg gatcagctga aaactaacat cgaaagtttg catctggagt taagcgaaga
     1081 cgtattagct gaaattgaag cggtgcatca ggtttatact tatccggcac cataagatta
     1141 acgccggtta aaaccggcgt taaatattaa tgacggcgct gccagatcca cagcgccgtt
     1201 attgccagcg caaacagcgc accgaagcca atgccaatgg gcacgaccgg gatgcctacc
     1261 attaccgcca gcgagtaaat gcccagcatc aacaacatgg cgctgttttc gccaaggttt
     1321 tgtactgcaa tcgcattccc cgccccgacg ctttttttac cccgctcctg tagcaacgca
     1381 ttgagcggaa cgacaaaaaa gccccccatc acgccaatca gcatcaacaa ggcataggct
     1441 ggcagcagct cgtgttgcag ggaaaaaatc agtaccacca cgccaatcaa aatcccggct
     1501 ggcatacagc gtgacatggt ttccagcgta actaacttcg ctgccgcacc tgcgccaacc
     1561 acgataccaa tcgctaccat cgcgttgaga taggtgggcg tagcgttatc ggtaatgccc
     1621 agcgccaccg gtacccacag caccaacagg aaacgcagcg tgacacccgc tccccagaat
     1681 aaactggtgc ccaccagcga aaaacgcgtt tcaccattgc gccatagcga ggtgcaggca
     1741 ttcaggaaac tgcgggtcat gttgatgaga ttccaggact gccccggacg cgccgccgcc
     1801 agtttgggaa tgtagatatt ggcaacgacc gcaccaccgt aggccagtgc gcatgcggcc
     1861 agggcgacga ggacatgcca gtcagccagc acaccaccgg ctacggaacc gagcaaaatc
     1921 gccgctatgg tagaagcttc cattaaaccg ttagctttca ctaacttact acccgtggtt
     1981 aattcgccga gaataccgta tttcgccggt gaataggctg cagcaccaac acccaccagc
     2041 gtatagccga gaaacggatt gataccaaag cagatactgg ctgcgcccag cagcttcagg
     2101 ccgttggcaa acatcatcac ccggcctttg gcgaagctat ccgccacctg cccgacaaac
     2161 ggcgcaaaaa gaatgtaagc acctacaaac accatttgca ggatgggctg gctccactcc
     2221 ggatagaact gcgctttcag taacgccaga gtggcaaaca gtagggcatt atcgccaaac
     2281 gcagagagaa actgcgccac gataaccgct ttcatcccct tcgaccacaa cgaagtgtta
     2341 gtgtgcactg actcactcat cgtgttgttc cgcttcgtct acccagcttt tcaacgtgac
     2401 aaagtcaggt ttgccgctgc caagtaatgg catctgtttc agatagcgaa tatcgcgcgg
     2461 tacagcaagc tccggtacgc cgtgctcgcg ggcatactgt tgcaacttat cgcgcgtcag
     2521 ttcgttatct gtggtgaaaa gcaccagtgc ctcgcctttg ctggcatcgc tcttaatcgc
     2581 agtggcatgg actttatctg gcgaaacacc aagtgccagt tgttccacca tttccagcga
     2641 caccatttcg cctgcaattt tggcaaagcg ttttgcgcgg ccctgaatct gcacaaagcc
     2701 ctgctcgtca aaacgcacaa tatcgccagt gtcataccag ccgcgctcca tttcgccgcg
     2761 aacattctcg gcggtgggca cttccagtac acctggcttc tccacccgca gatagccgtt
     2821 cattatgttc ggccctttca gttgcaggcg tccgccctct tcgataccag ggaccgacaa
     2881 caggcgcgca tccattcctg gtagaatacg ccctaccgta ccgggtttcg ccgccatcgg
     2941 tacgttgata gaaacgacag gcgcgcattc ggtcacgccg tagccttcaa ggatgcgcag
     3001 gccaaattta tcctgccaaa gctgtttggt actttcttgt aatttttctg cgcctgccac
     3061 cacatagcgt agacgataga agtcatacgg gttggcgaaa cgcgcgtagt gaccgaggaa
     3121 agtcgaggtg ccgaacaaca cggtgcaact gcggtcatac accaactccg gcacaatacg
     3181 gtaatgcagc gggcttggat aaagaaacac ttctgcacct gtgagcagtg gcgtaaacag
     3241 gcctaccgtc agcccaaagg agtgaaacag cggtaacgcc gacataaagc gatcgttggt
     3301 ggtgaagtcg gcaatcgttt taatctgctc gacgttcgcc agaatgcttt tatggctatg
     3361 gacgacgcct ttcggatggc cttcagaacc ggaggtaaag aggatcagcg cctcttcttc
     3421 cggctgctgt tttacctgtg ccagacgtgg catcagcaga tgcgcgaaga tccatacttt
     3481 gtcggcagtg gtgacatctg cttttaaatc ttccagatag acccagcgca cctgagtaag
     3541 ttgctccggc agatgccaga gtttgccttt atcgagaaac tggcgggaag tgaagatggt
     3601 tttgatttca gccgccgtaa tagcactggt cagccctttt accccggcag tgtagttcat
     3661 cattgcggga atgcggcgac gggcgatggc cccaaaaatc actgccgcac tgatgcctgc
     3721 attgggcagc attaagccga tgcgttcgcc ttcaacactg tatttttcaa ggatgcgtcc
     3781 aacaaacagc gttttcgtaa gcaatttgcg ataggagtct ggggtaaagt tgacgtcttc
     3841 gacacatttc ttcccggctc cgaagcggta cattgcactc agtaaagatt cgtacagcgt
     3901 ttcacgcggg cgcaccgcca ttcgcgcttc catcattatt tgatgcagca tttcgccagc
     3961 gattttgcga cggtcacggg cacgcggcgc atccggcatc tccacctgcg ttggtggcaa
     4021 aatatgcaga gtaatttgcg ggaacaagcg acgtttaacc agacctttca ggcggctgaa
     4081 gtgcgtaagt tccgcccctt caatacgcac aggaataacc gttgcaccag acttcgccgc
     4141 gacaaaaccc gcgccatcgt agattttcat cagcgagcct gtggtggtga tgcgtccttc
     4201 agggaaaatc accactggtc ggccctgttc caccagacgt accagatgtt taatcgccat
     4261 aggttgcgtc gggtcgagag gaacaaagtc gataaatgat ttcagccaac gcatatacca
     4321 ctgttggctt attgaggtgt aaacggcaaa cactggacgc acaggtaaaa acagtccaag
     4381 caaaatgcca tcaataaaag agacgtgatt aggcgtaatt agaacgcgct cgcccttcag
     4441 tgcctgggtg tcacccgtaa cgcgaacgcg atacaaaaca cggcacaaat ttcgaaaaaa
     4501 gctaaaaagc attccaactc cctttgctct gattcagtaa aagcgaatgg agggagatta
     4561 cacgagataa agaacgcgag cgacagtaaa ttaggtgcga aaaaaaacct gcgcatccgc
     4621 gcaggttggt gcaagagaca gggtacgaag agcgtaccga ataatctcac caatcaatac
     4681 ctctgggatc ttgattgtgg tctgcacgac gactcttcgc cagcgagaaa acgcaaagga
     4741 atgaagggaa atgcaacgag gtgtgtaaat tgtcggttac tgttacagat tgatgaccgg
     4801 caaaaaaaaa cctgcgcatc tgcgcaggct ggtgtaattc atgtgctcaa cccgaagttg
     4861 acttcaccta tcaatacctc tgggatcacc actttagcaa cctgaagcca aacgccacca
     4921 gcagtcaatc gcaacagcat ttcgcaaagt gtaaccaaag gtttgcaatc tctttttttg
     4981 tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa acacgccgcc ccttgaacca
     5041 acgggcgttt tccgtaacac tgaaacaatg taagcgttta cccactaagg tattttcatg
     5101 gcgaccataa aggatgtagc ccgactggca ggcgtttcag tcgccaccgt ttcccgcgtc
     5161 attaataatt cacccaaagc cagcgaagcg tcccggctgg ctgtgcatag tgcaatggag
     5221 tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac cactgaaacg
     5281 gtcggtctgg tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt gaaagcggtc
     5341 gaacaggtgg cttatcacac cggtaatttt ttattgattg gcaatggtta ccacaacgaa
     5401 caaaaagagc gtcaggccat tgagcaactg atccgccatc gctgtgctgc gttggtcgtc
     5461 catgccaaaa tgatcccgga tgccgattta gcctcattaa tgaaacaaat gcccggtatg
     5521 gtgctgatca accgtatcct gcctggcttc gaaaaccgtt gtattgctct ggacgatcgt
     5581 tacggtgcct ggctggctac gcgtcattta attcaacaag gtcatacccg cattggttat
     5641 ctgtgctcta accactctat ttctgacgcc gaagatcgtc tgcaagggta ttacgatgcc
     5701 cttgctgaaa gtggtattgc ggccaatgac cggctggtga catttggtga accagacgaa
     5761 agcggcggcg aacaggcaat gaccgagctt ttgggacgag gaagaaattt cactgcggta
     5821 gcctgttata acgattcaat ggcggcgggt gcgatgggcg ttctcaatga taatggtatt
     5881 gatgtaccgg gtgagatttc gttaattggc tttgatgatg tgctggtgtc acgctatgtg
     5941 cgtccgcgcc tgaccaccgt gcgttaccca atcgtgacaa tggcgaccca ggctgccgaa
     6001 ctggctttgg cgctggcgga taatcgccct ctcccggaaa tcactaatgt ctttagtccg
     6061 acgctggtac gtcgtcattc agtgtcaact ccgtcgctgg aggcaagtca tcatgcaacc
     6121 agcgactaac cgcagttaaa gcaattccag cgccagtaat tcttcgatgg tctggcgacg
     6181 gcgaatcaac cgcgcctgac cattatcaaa cagaacttct ggtaacagcg gacggctgtt
     6241 gtagttggat gacattgatg cgccatatgc ccctgtatca tgcagtacca gataatcacc
     6301 cgccttcact tccggcaagg cgcgggtttc aacatttccc ccttcctgct gggtaaagac
     6361 atcgcccgat tcacataatg gcccggcgac gacggtttcc accgttggcg cgtgttccag
     6421 agaacgacca tcagctgcca gggcactgat atggtggtaa ctaccgtaca ttgccgggcg
     6481 catcagatcg ttgaacccgg catcaaccag cacaaagtgg cggctaccca tttgtttgac
     6541 gctccgcacc tgagtaatta acacgccaga ctgcgctacc agaaagcgac ccggttcaat
     6601 ttccagtttc acagggtggc ccaaatggcg agcgatttgc tcacgcgcgg cattccacag
     6661 accataataa tgttcggtat caaccgcctc ttcaccctgt tgataaggaa tagaaagccc
     6721 accgcccgca gaaatagcct gtaaatcctg accgaattcg atgacctgac gtaccatagc
     6781 accacacacc tgttccagat gggcataatc aacgccagaa ccaatgtgca tgtgaatgcc
     6841 gaccagctgc agatgatgac gttgtatcac gtccagtgcg gcgggcagat cggtgtacca
     6901 gataccgtgc ttgctgtttt cgccaccggt attggttttt tggctatgtc cgtgaccaaa
     6961 ccccgggtta acgcgcagcc atacccgatg ccctggcgaa acctggccca gttggtcgag
     7021 catatcaaca gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt caagcgtcgc
     7081 ctgatcgata acatctgccg taaaaacaat atcatcgggg tgcgtttgcg gattgtaacc
     7141 cgccgccaac gcacgctcta tttcgcctaa cgagacggaa tccactttca cgccctgctc
     7201 acgcattaat cgcaaaatat gaatattgga acaggctttc tgtgcaaagc gcaccacatc
     7261 aaactgtttc agcgctgcaa tctgccgacg aataatttgc gcatcgtaga cccacaccgg
     7321 gcagccaaat tcagcgggca aacgcagcag attttcggcg gtgagatcgg tatcggtgct
     7381 gaacagtgaa tgtggcataa caaactccag atgagtgctt ttttatgatt acgccacatc
     7441 ataaaaagaa taaaaaatat cgatttatgt cgagtctatg caaaaatgat atagattacc
     7501 ggattgcgag agagcgctaa tggccgccgt taacttacgt catattgaaa tttttcatgc
     7561 ggtaatgacc gccggaagcc tgactgaggc agcacacctg ctacacacct cacagccaac
     7621 cgtcagccgc gaactggcgc gctttgagaa ggtgatcggg ctaaaattgt ttgagcgcgt
     7681 acgtggacga ttacatccta ccgtgcaagg actgcgtctg tttgaagaag tgcaacgatc
     7741 ctggtacgga ctggatcgca ttgtcagcgc cgcagaaagt ctgcgcgagt ttcgccaggg
     7801 agaactgtct attgcctgcc tgccggtctt ttcgcaatct tttttaccgc agctcctgca
     7861 accctttctg gcacgttatc ccgatgtcag cttaaatatc gtgccccagg aatcaccgct
     7921 acttgaagag tggctctcgg cccagcgtca tgatttagga ctcactgaga cactccatac
     7981 gcctgcggga acagaacgta ccgaattact ctctttagat gaagtgtgtg tgttacctcc
     8041 gggtcatccg ctggcggtaa aaaaggtatt aacgccggat gattttcagg gtgagaacta
     8101 catcagcctt tcccgtactg acagctatcg ccagttgctg gatcagctat ttactgaaca
     8161 tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtct gcgcaatggt
     8221 acgggcgggg gtaggtgttt cggtggttaa cccactcacc gcactggatt atgcggcaag
     8281 cggtttagtg gtacggcggt tcagtattgc ggttccgttc accgtcagcc tgatccgccc
     8341 cctgcaccgc ccgtcatcag cgctggttca ggcgtttagt gagcatttac aagcggggtt
     8401 accgaaactg gtcacttctc ttgacgcgat tttgtcgtca gctacgacag cataaaagcg
     8461 acagcatcct cggcatggat cgccgcagta tcaaacacag gcaaaacact gcgctcttct
     8521 ggaaccaata aaccaatttc tgtgcagcca aaaatgacac cctgcgcccc ctgttctgca
     8581 aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt cggtaaattg ccccagacac
     8641 agttcttcaa aaataatctg attaatcttc gcccgttcat ccgcttccgg aataagacaa
     8701 ttgatggaaa attattccgt cagccgcccg cgataaaaat cctgttccat ggtgtaacgc
     8761 gtacccagca gcgccacacg agtcattccg gccccggtaa ttgcacgtcc ggtggcatcc
     8821 gccactttat gcatcgtatt ggtacataac acaatacctt ctgcgcccgc ccgctgtaag
     8881 ccaagcgccg cctcagccag aatgtccccg gttttatccc attccccgcg acgctggcac
     8941 tcttctattt catgaaaatc gacgctatgt agcagcactt gcgcagagtg aagcccacca
     9001 agccgctgtt taatgccttc atttatcaga cgatagtaag gaatggtgga ttcccagctc
     9061 attcctccca gcaaaccaat tgttttcatt cctctctctc ctgatgtgtg ttaccgcaac
     9121 aaggctgaat aaggaacgag acaaacgcct caacggccag gtgcccaatc tctattaacg
     9181 aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat cagacgccga tatttctcaa
     9241 cttctcgcct gccatcagtt tgcgttcgat atgttccagc gtgacatttt tggtttccgg
     9301 aatgagccag aaagtgatgc ccacaaacgc aatgttcagc gcagtgtaga gccagaacgt
     9361 accggcagca ccaatgctgt cgagcagcgt caggaaggtc gcgccgataa tcatattcga
     9421 cacccagttc gtggtggtcg aacaggtaat accgaaatcg cggcatttca gcggctgaat
     9481 ttcagagcac aggatccaca ccactggcgc ggcgctcatc gcataaccgg caatacacat
     9541 catcgtcatg ccaacagaga gccaggacaa gccactggaa gccgtaccgt tatcaaactg
     9601 catcaggcaa tagcccagca ccagagtgcc taacgccatc acgctgaaac caattttcag
     9661 agccggttta cgccctgctt tatctaccgt aaacaccgca ataaaggtgg cgaacataaa
     9721 ggtcagccct acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat
     9781 tttgaagata cgcggcgcgt agtacatgat gatgttcata ccggtaaact gctgcatcgc
     9841 ctgcaacaac ataccgagaa acacagcacg acggacgtta cggttgatct taaacagtgc
     9901 ccaaccgccc tgttttaact tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt
     9961 ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc tcaatatgac gccccttttc
    10021 cgccagccag cgcgggctat ttggcaggaa gaccaccaga ataatcagca ggactgctgg
    10081 taaagcaaga acccccaaca ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga
    10141 taaaaacgcc agcacgatgc cgagtgtgac catcaactgg tacatactga tcatcttacc
    10201 gcgaacgttt tcacttgcca tttcagaaag atacagagga gcggtgtaag acgcgatccc
    10261 gacagcaatg cccagcacca cccgagcggc gattaacatc tctacgctgg tcgcaaaagc
    10321 ggaccctata gaaccgagta caaacaggat ggcccccgcc atcaggctgt atttacgccc
    10381 caggcggaac gacagccaac cattaaacag cgcaccaatt gctgcgccga gcatcatgct
    10441 actaaccacc cattcctgca aacgactggt cagcacaaag tgatcggtaa tgaacggcaa
    10501 cgctccggcg attacgccga tatcaagacc aaataacaat cctgcgaccg cagcagctac
    10561 cgaaacaaac atattcatac gccgcgtatc ccgcaaagaa cgtggcgtta aagcagattc
    10621 cgtattgata gtaaccattt tttcctgcca gcagagagta agacatagtg aaaaaatacg
    10681 tgaacaactc acacaggtgt caggtcggaa acagcataaa tatggattaa attgctgcga
    10741 catgtcgtta tgtgatggat attccaattt tcaaattaag ttgaattatt gagattatta
    10801 ttaaccacct aattttacag cagataaaat tcataaagtt cattaattga taattgatat
    10861 ggattatttc ataaccatga tatgaattat gatgacctac aggtataaaa aaccctgcca
    10921 tgaggcaggg tcataaaagt aagaggaatg aattaacgag ccagccaacc gccatctacg
    10981 gcaatggtat aaccattcac ataatctgaa gcgctggagg caaggaacac tatcggcccc
    11041 atcaggtcac tcggcagtcc ccaacgacca gctggaatgc ggtcgagaat ttccgcgcta
    11101 cgttgttcat ctgcccgtag ttgttgagta ttgttggtcg ccatgtaacc cggagctatc
    11161 gcattaacat taatgttgtg tttagcccat tcgttcgcca tcaatcgcgt cacgcccatc
    11221 acgccgcttt ttgatgcggt ataagaaggc acacggatcc cgccctggaa ggagagcatt
    11281 gacgtgatat tgataatctt gccgccattg cgttgcgcga taaagtgttt cgccgctgcc
    11341 tgagacatga agaatacgct cttgatattc aggttcatga catcgtccca gtctttttcg
    11401 ctgaactcga gagcatcttc gcggcgaatc aatccggcgt tattcaccag gatatcaata
    11461 tgaccaaact cagctaccgc gcgatccagc agtgctggaa tgccatcaat ctttcgcaga
    11521 tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga cctgcttgat ggtttcagtc
    11581 ggttcaacga tgttaatgcc aacaatgtca cagcccgctt gcgccagccc caacgccatc
    11641 ccctggccca gtccagtatc acaaccagtg acgaccgcaa ctttaccttc gagagaaaat
    11701 gcacttaaaa tcataacaat accttattc
//
LOCUS       DAJTVJ010000129        11692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000129 DAJTVJ010000000
VERSION     DAJTVJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11692
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.129"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..382
                     /locus_tag="OO448_003441"
     CDS             <1..382
                     /locus_tag="OO448_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6338565.1"
                     /translation="EQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKP
                     YYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMV
                     YEIYGMWIDDMNDEQIAMLNARLS"
     gene            434..1369
                     /gene="ttcA"
                     /locus_tag="OO448_003442"
     CDS             434..1369
                     /gene="ttcA"
                     /locus_tag="OO448_003442"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6338566.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1498..2871)
                     /gene="dbpA"
                     /locus_tag="OO448_003443"
     CDS             complement(1498..2871)
                     /gene="dbpA"
                     /locus_tag="OO448_003443"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6338567.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3349..4332)
                     /gene="zntB"
                     /locus_tag="OO448_003444"
     CDS             complement(3349..4332)
                     /gene="zntB"
                     /locus_tag="OO448_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6338568.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4587..5819
                     /gene="dgcM"
                     /locus_tag="OO448_003445"
     CDS             4587..5819
                     /gene="dgcM"
                     /locus_tag="OO448_003445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR6338569.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5840..6403)
                     /gene="smrA"
                     /locus_tag="OO448_003446"
     CDS             complement(5840..6403)
                     /gene="smrA"
                     /locus_tag="OO448_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6338570.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6733..7641)
                     /locus_tag="OO448_003447"
     CDS             complement(6733..7641)
                     /locus_tag="OO448_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6338571.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7817..9127
                     /gene="abgA"
                     /locus_tag="OO448_003448"
     CDS             7817..9127
                     /gene="abgA"
                     /locus_tag="OO448_003448"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCR6338572.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPTSALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9127..10572
                     /gene="abgB"
                     /locus_tag="OO448_003449"
     CDS             9127..10572
                     /gene="abgB"
                     /locus_tag="OO448_003449"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR6338573.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10609..>11692
                     /locus_tag="OO448_003450"
     CDS             10609..>11692
                     /locus_tag="OO448_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6338574.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFN"
BASE COUNT         2969 a         2971 c         2898 g         2854 t
ORIGIN      
        1 ggagcaatac aaactgaccg ggcatcatcg caaaagcgaa atcacttttt atcatcggga
       61 gtacggcaga actgaaaagc aaaaactgca ttttgttttc atgcccaggg tgtgtaacga
      121 aaaacagaaa ccttattact cggtaagcag tttgggtgca agatggaatg cagcagtaaa
      181 acgtgctggt attcgccgcc gtaatccgta ccatacgcga catacttttg cctgctggct
      241 gttgacggca ggagcgaacc cggcatttat agccagccag atggggcatg aaactgcgca
      301 aatggtgtat gaaatttacg gtatgtggat tgatgacatg aacgacgaac agatagccat
      361 gttgaatgcg cggttatcgt agttgcaaag tttgccccca atttgcccca tttagtacca
      421 gagaactgaa ataatgcaag aaaatcaaca aattacaaag aaagaacaat acaacctgaa
      481 caaattacaa aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat
      541 tgaagaaggc gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct
      601 ggagattctg cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa
      661 cctcgatcaa aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct
      721 gggcgttgag tacaagattg tcgaagagaa cacttacggt atcgtgaaag agaagattcc
      781 agagggcaaa accacttgct cactgtgttc tcgccttcgt cgcggtatcc tttatcgtac
      841 cgcaacggaa ctgggggcga cgaagatcgc gctgggtcac catcgtgacg atatcctgca
      901 gacgctgttc ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat
      961 gagcgatgat ggcaaacata tcgttattcg tccgctggcc tactgccgcg agaaagatat
     1021 tcagcgattt gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca
     1081 gcctaacctg caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc
     1141 agggcgtatc gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga
     1201 taccaacctg ttcgatttca aaggcatcac ccacggttct gaagtggtta acgggggtga
     1261 tctggcgttt gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga
     1321 tgaaaatcag ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatggcg
     1381 cccgatgcgc aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc
     1441 gcatccggca atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta
     1501 ttttaataac cgcacccggc tcgtttttcc tttaatcttc ccgccctgta actgtttcca
     1561 tgctttatga gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat
     1621 tttgccaata tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc
     1681 cgggcgcatt ttggcttttt tcccaccatc gatacacaac gttgccattt ctgcttccag
     1741 cggcacaatg gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc
     1801 agaaatgata ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct
     1861 atttcctgca cgagctgtac ggccgatgcg atgtacatga acttcagggt cccacgccag
     1921 ctcaaagttc accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt
     1981 cgcaaccagt acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgatcgcg
     2041 ttgctccaaa tcgccgtgta atgacaatgc actttgccct acttcattca gcgcgtcgca
     2101 gacagcctgg caatcttttt tggtattgca aaacaccacg caagaggatg gctgatgcaa
     2161 gcttaataac cgttgcaaca gaggaatttt gcctttgctg gatgtctcat aaaattgttg
     2221 ttcaatgggt ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac
     2281 tcgtccgctg attacggcaa tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg
     2341 agatgcaggc gcaaaacgga tgacatcatc aatggcatcg ctaaatccca tatccagcat
     2401 gcggtcggcc tcatccatca ccaaagtatt cagcgcatcc agtgataccg tgcctttttg
     2461 caagtgatcc agcaaacgac ccggcgttgc cacgataata tgcggcgcat gttgcaacga
     2521 atcacgctgc ataccgaacg gttgaccacc gcacaacgtc aaaattttgg tatttggcag
     2581 aaaacgcgcc agccgacgca attcacctgc cacctgatcc gccagttcac gcgtaggaca
     2641 cagcactaaa gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc
     2701 aaaagccgcc gttttgccgc tgccggtttt cgcctgcacg cgaacatctt ttccggcaag
     2761 gatcgccgga agcgcggcgg cctgcaccgg cgtcatagtt aaataaccca actcattaag
     2821 gttcgtgagt tgggcgggag gcaaaacatt cagggtagaa aaagcggtca caatctattc
     2881 tcgtggtcat cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc
     2941 gacaatttaa tcggttcttc atcgggtggt gggtcaggca tgggttgcgg gcgagggatc
     3001 ggatcgggca ctggaacagg atcgccagga atcggttcag ggacaggaat ttgcaaataa
     3061 ataagtgtcg tcatatttcc ctccggtcat tgggtggact cttaaagggt agacgctgat
     3121 aaataacagg caaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct
     3181 atgcagtaat tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt
     3241 tgcattcacc tgcaagggag atattgccct gaatgggtag agagtttatt gacttcgctc
     3301 aaactttgcg gcgtttttgt atacagacag ccggaaaaat tgcttttgtt acaaccattt
     3361 actacggtgc aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat
     3421 tgaaaatccg aattgccacc cgccgccagg gatcccacca aggttgacgc caaataaccc
     3481 ggtcagaaag gtactgggta aaaagaccat tgccatcaac gacattgtat aggtacgacg
     3541 agctaaattt tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat
     3601 acaggcgtcg atttcgtcaa ggccgcgccc aaggcgatcg gcaatatcct gcatccgacg
     3661 gcgttggtca tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg
     3721 ttgcggtgcc atatagcgac gcatcacaat taattgtttg cgcagcagag ccaggaatcc
     3781 acgcggtgga atttgctgat caaggagatt atcttcaagg tcgataattt tatcgtgcag
     3841 ctgctcgata aattcgctgg aatgatcggt caacgcatcg cacacatcca ccagccaccc
     3901 tccgcaatcg gtcggacccg tgccctcttc cagatcgctc accacatcgt ccagcgccag
     3961 cactttgcgt tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac
     4021 cagttgatcg gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc
     4081 ttcaccgaga cggctgactc ggggccgcgt gctctcgccc gccagcgcat cacgtacgtt
     4141 attgggaagc agcggtgttg tcgccagcca ttgggcgcta tcatggtgta cataattaag
     4201 gtggagccag cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc
     4261 gcctctacca tccagcatcc aggcaaatac tgcatccggg acattaacgt ccgatccctt
     4321 aatcgcttcc acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc
     4381 tgttacgcaa catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat
     4441 tgaatacaaa aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc
     4501 tatcattatc agaattatct gatcatatga cgtggctttt ttgcgatcgg atagcaacaa
     4561 aaattgataa aaataacggg atctcaatga ttacgcacaa cttcaatacc ctggacttac
     4621 tcaccagtcc tgtctggatc gtttcgccct ttgaggaaca gttaatttat gccaatagcg
     4681 cggcgcgact gttgatgcaa gacctcacgt ttagtcagct acgaaccggt ccctattccg
     4741 tctcctcaca aaaagaactg ccgaaatacc tctccgatct gcaaaaccaa cacgatatta
     4801 tcgaaatcct cactgttcag cgtaaagaag aggaaacagc attaaactgt cggcttgttt
     4861 tgcgaaagct gacagaagca gaaccggtga ttattttcga aggtatcgaa gcgtcggcaa
     4921 cgctgggttt aaaagccagt cgctcggcaa attatcagcg caaaaaacaa ggtttttatg
     4981 cgcgcttttt tctgactaac tctgcaccaa tgttgttgat tgacccctca cgagatggcc
     5041 aaatcgtcga tgctaacctc gccgcgctca atttctatgg ttataaccat gaaacgatgt
     5101 gccagaaaca tacctgggaa ataaatatgc tcgggcgtcg cgtcatgcct atcatgcatg
     5161 aaatttcgca tttacccggt ggtcataaac ctctgaattt tattcataag ctggcggatg
     5221 gttcgactcg tcatgtgcag acctacgccg ggccgattga aatttatggc gacaagctca
     5281 tgttatgtat tgtacatgat attactgagc aaaaacggct ggaggagcag ctggaacatg
     5341 ctgcccacca tgacgcgatg accggattac tgaatcggcg acagttttat cacattaccg
     5401 agccaggcca aatgcagcat ctcgccatcg cccaggatta cagcttgttg ctcatcgaca
     5461 ccgatcgttt taaacacatt aacgatctct atgggcattc taaaggtgat gaggtgttat
     5521 gcgccctcgc ccgcaccctc gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg
     5581 gaggcgaaga gtttgtctta ttgctaccaa gaaccccact ggataccgcg ctttcgctgg
     5641 ctgaaactat ccgcgtaagc gtggcaaaag tgagtatttc gggcttacca cgctttaccg
     5701 tcagcattgg tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg
     5761 ttgatgatgc tttgtatcgg gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag
     5821 ccgcggatgc gtctcgagat caacgactgc gcttagcgtg gcgctcccag ttttcttgct
     5881 tcgcctgcgc cgttttacgt agtgcgacgt aacacgcccc gctgccgcca tgatgcggta
     5941 gcgcagtgca atatgcctga acatcatcaa attcggtcag ccagcgcgcc acatagctgc
     6001 ggacaatatt ggcatgcgat ttatcatccc gtcctttgcc atgaataatc agcacgttac
     6061 gcaaaccatc agccatggct tgttgaatga aactgaacat cattttgcgg cactcttcca
     6121 ccggctggcg caaaagattc aggctcgcct gttgcggata tttaccactg cgcagcttat
     6181 ccagcacccc atgttgcaac ccttcccgcc gaaactccag cggctgactt agtgggatga
     6241 tgtcgagaaa tccggtggtg aggaaattat caagctgcag cgtgtcgata cgctgcggcg
     6301 cacgttggtt acgcgttgga tgccagtgga catcggtagc acgtttcagc ggctggacat
     6361 cttccatggc gtcaagaaac agcgatttgt cgtcaaggtt catgttacat cctcccgcaa
     6421 ttaagagcgc gatatgataa ccagaccggg tcggtccaac aacgtattac ccaaaattcc
     6481 agtaataagt tccaaatatt gccgatattt taagcaaaat gcttatgcat gattattcat
     6541 tcacgatatt aataatgtaa cttatatttt cgtgaaatct gtcactgaag aaaattggca
     6601 actaaaggtt aaaaccgtta taacacagtc accggcgcag aggagacaat gccggattta
     6661 agacgcggat gcactgctgt gtgtactgta gagtctggcg gatgtcgaca gactctattt
     6721 ttttatgcag ttttaacttt gcagatagcc gcattctcgc cggaattgcg tgattaatga
     6781 cgcggtcagt ggtgtctggc gactatcacg ccgctgaatc aaataatagg ccgctttcgg
     6841 taaaatttcg ctaaccggca acatcaccag cccctgtccg tgcaagggat cgcagcccat
     6901 ttcttcaggc agtatgctga gaaaatcgct ttttgccacc agactgatac aggctgagaa
     6961 cgtctcgcag actacaccga cctgtggcgt ttgcgcctga tcgtcaagca attcactcaa
     7021 ctgtttgtag taactgccgt gtggcgtcgg cattgtccag ctgtaatcca gtaactgttt
     7081 gatcgaacgg gcaccaatgg cggggtgtcc cgggcggcaa aagatcgcga attgcttttc
     7141 cagtaatttc tcaaaagtaa attcgtggtc gtacggtccc tgataatagg tattgatggt
     7201 gaaatccaat tctccctgac gcaattcatt aatcatcgac accagttgcc cttccataat
     7261 gcgtactttt acctgcggat gctgctgatg aaaacgagat atgacagctg gcatcagact
     7321 gcgggaaata ctggccccca tgccgatatt aatctgccct gccagttgcc cttgtcgttg
     7381 gcgaatatcc tcttgggctg cgcgcagctc ttcaagaata agactggcgt gctgataaaa
     7441 actttcaccg gcatcagtta acgtcacgcc tttactacgg cgaaaaaaga gttgcgccgc
     7501 taatccttct tccagctcct gaatagattt actcagtgcc ggttgcgaca tattcaacat
     7561 tcggctcgct ccgcgaatgc tgccctgacg agccacttca acaaaagccc gaatttgatg
     7621 aatttttacc tgaaaagcca tgacgccacc gataaccgtt atttatcaga ccaaagaaac
     7681 tggcatctac tttaatgcag atgattgtgt cagggtaatt tatgaacggt taaaactgtg
     7741 aaaaatcagt tagtgataag taaaaactat cgctacgtga accgggtcac actttttact
     7801 gatgacggga aaggttatgg agtctttgaa tcaatttgtt aattcgcttg ccccaaaatt
     7861 atcgcactgg cgacgtgatt ttcatcacta tgcagagtct ggctgggtgg aattccgcac
     7921 tgccaccctt gttgcggaag aattgcagca gctcggctat tcactggcgc tgggccgcga
     7981 agtagttaat gaaagtagcc ggatgggact acctgatgaa ctcactctac aacgcgaatt
     8041 cgagcgcgct cgtcaacagg gtgcgctagc acaatggatt gcggtttttg aaggtggttt
     8101 cactggtatc gtcgccaccc tggataccgg tcgccccggt ccggtgatgg ctttccgtgt
     8161 cgatatggac gcgctggatc tcagtgaaga gcaggatgtc agccatcgcc cctaccgcga
     8221 cggttttgcg tcatgtaacg ccggaatgat gcatgcctgt ggtcatgatg gacataccgc
     8281 cattgggctt gggctggcgc atacccttaa acaattcgag tccggactac atggcgtcat
     8341 caaactgatt tttcagcctg cagaggaagg tacgcgtggc gcgcgggcga tggtcgatgc
     8401 aggtgtcgta gatgatgttg attattttac tgccgtgcac attggcactg gcgtacctgc
     8461 gggcaccgtg gtgtgcggca gtgataattt tatggcaacc accaaatttg acgcgcactt
     8521 caccggtacc gccgctcacg caggcgcaaa accagaagac ggtcacaatg ccttgttggc
     8581 ggcagcacaa gccactcttg cactgcatgc aatcgccccg cacagcgaag gagcttccag
     8641 agtaaacgtg ggcgttatgc aggcaggaag cggtcgtaac gttgttccta cctcggcgtt
     8701 gctgaaagtg gaaacacgcg gggccagcga cgtcattaat caatatgttt ttgaccgtgc
     8761 acaacaagcg attcagggcg cagcaaccat gtatggtgtc ggcgttgaaa ctcgtctgat
     8821 gggtgcagtt accgccagtt ctccttcgcc gcaatgggtc gcatggttgc aaagtcaggc
     8881 ggctcaggtc gcgggggtca atcaggccat tgaacgtgtt gaagcgcctg cgggttccga
     8941 agatgccaca ttaatgatgg cccgcgtgca gcaacatcaa gggcaagcct cctacgtggt
     9001 gtttggcaca cagctggcgg caggtcatca caacgaaaaa ttcgattttg acgagcaggt
     9061 tctcgctatt gccgtcgaaa cgctggcgcg caccgcgctc aattttccct ggacgcgagg
     9121 tatctgatgc aggaaatcta tcgttttatc gacgatgcga ttgaagccga tcgccaacgt
     9181 tataccgata ttgccgatca aatctgggat catccagaaa cacgttttga agagttctgg
     9241 tcagcggagc atctggcttc ggctctggaa tctgcaggct tcaccgttac ccgcaacgta
     9301 ggcaatatcc caaatgcctt tattgcttcg tttggtcaag gcaaaccggt tatcgccctg
     9361 ctgggagaat atgacgccct ggcaggttta agtcagcaag ctggttgcgc gcaacctaca
     9421 tccgcgacgc ccggtgaaaa tggtcacggt tgcggacaca atttgcgggg aaccgccgcc
     9481 tttgccgctg caatagccgt caagaaatgg ctggaacaat atgggcaagg cggcacagtg
     9541 cgcttttatg gttgtcctgg cgaagaaggc ggctcgggta aaacgttcat ggtccgcgag
     9601 ggggtatttg atgatgtgga tgcggcactc acctggcacc cggaagcctt tgccggtatg
     9661 ttcaataccc gcacgctggc aaacattcag gcatcatggc gctttaaagg gatcgcagca
     9721 catgccgcga attcccctca tttgggacgc agcgcccttg atgccgtaac gttgatgacc
     9781 actggcacca acttcctcaa cgaacatatt attgaaaaag cgcgcgtaca ctatgccatc
     9841 acaaatagcg gcgggatctc gcccaacgtg gtccaggcgc aggcagaagt gctttatctt
     9901 atccgcgccc ccgaaatgac cgacgtgcag catatttatg atcgggtcgc caaaatcgcc
     9961 gaaggtgcgg cattgatgac cgaaaccacg gttgaatgcc gcttcgacaa agcctgttcc
    10021 agttatctcc cgaatcgcac cttagaaaat gccatgtacc atgccctatc ccattttggt
    10081 accccggaat ggaactccga agaactggct tttgcgaaac aaattcaggc tacgctcacc
    10141 cccaacgatc ggcaaaacag tctgaataat atcgccgcaa ccggtggcga aaacggcaag
    10201 gcttttgcac tacgtcatcg tgaaacggta ctggcgaatg aggtcgctcc atatgccgcc
    10261 accgataacg tgcttgcggc atcgactgat gtcggcgacg ttagttggaa actgcctgtt
    10321 gcccagtgtt tcagcccctg ttttgccgtc ggtacaccgc tacatacgtg gcaactggtt
    10381 agccaggggc gaacatctat tgctcataaa ggaatgctgc tggcggcgaa aactatggca
    10441 gcaaccacag tcaatctctt cattgattca gggctattgc aagaatgcca acaagagcat
    10501 cagcaagtaa cggacacgca accgtatcac tgccctatcc cgaaaaacgt gacaccgtca
    10561 cctttaaaat aacaacaaca acgcaaacac aacaaccgag gaatgcccat gagtatgtca
    10621 tccataccgt cgtcctccca atccgggaag ctctatggct gggtcgaaag aattggtaac
    10681 aaggttctcc atcctttttt gctctttatc tatttgatta tcgtactcat ggtgacgacg
    10741 gcaattttgt cggcctttgg cgtcagtgcg aaaaacccga ccgatggaac gccggtggtg
    10801 gtgaaaaacc tgctcagtgt ggaaggatta cactggtctt tacccaatgt tattaaaaac
    10861 tttagcggtt ttgctccact tggtgcgatc ctggcgctgg ttttaggtgc cggtctggcg
    10921 gagcgcgtcg gcttactgcc agcgctaatg gttaaaatgg catcgcatgt taatgcccgc
    10981 tacgccagtt atatggtgct gtttattgct tttttcagcc acatttcttc cgatgcgacg
    11041 ttagtgatca tgctaccgat gggtgcgctg atttttctgg cggtgggcag gcatccagtt
    11101 gcaggtttac tggctgccat tgcaggcgta ggttgcggct ttacggctaa tttactgatt
    11161 gtcacaaccg acgtgttgct gtcggggatc agcacggaag cggcggctgc gttcaatccg
    11221 caaatgcacg tcagtgtaat tgataactgg tattttatgg ccagctccgt agtcgtactg
    11281 acgattgttg gcggcctgat aaccgacaaa atcatcgagc cacggttagg tcaatggcag
    11341 ggaaacagcg atgagaaact gcagacattg accgaaagtc agcgttttgg tttacgcata
    11401 gcaggtgtcg tatcgctact ttttattgct gcgattgcgc tgatggtgat cccggaaaac
    11461 gggatattgc gcgatccgat taatcacacc gtgatgccat caccctttat taaaggtatc
    11521 gtgccactga tcattctttt tttctttgtt gtctcgctgg cttatggcat cgctacccgc
    11581 acaattcgac gtcaggcgga tttaccgcag ttaatgattg aaccgatgaa agagatggca
    11641 ggatttatcg tgatggtttt tcccctcgcc cagtttgtcg ccatgtttaa ct
//
LOCUS       DAJTVJ010000130        11632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000130 DAJTVJ010000000
VERSION     DAJTVJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.130"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            486..1157
                     /locus_tag="OO448_003451"
     CDS             486..1157
                     /locus_tag="OO448_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCR6338575.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="OO448_003452"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="OO448_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="OO448_003453"
     CDS             2363..2923
                     /locus_tag="OO448_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6338576.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="OO448_003454"
     CDS             2925..3698
                     /locus_tag="OO448_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCR6338577.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="OO448_003455"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="OO448_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCR6338578.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="OO448_003456"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="OO448_003456"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCR6338579.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="OO448_003457"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="OO448_003457"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6338580.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="OO448_003458"
     CDS             complement(6457..6996)
                     /locus_tag="OO448_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCR6338581.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO448_003459"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO448_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCR6338582.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO448_003460"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO448_003460"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCR6338583.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OO448_003461"
     CDS             complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OO448_003461"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCR6338584.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OO448_003462"
     CDS             complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OO448_003462"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCR6338585.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGGYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11372..>11632)
                     /gene="mhpC"
                     /locus_tag="OO448_003463"
     CDS             complement(11372..>11632)
                     /gene="mhpC"
                     /locus_tag="OO448_003463"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCR6338586.1"
                     /translation="LENFVKSLEANPKQFPDFGPRLAEIKAQTLIVWGRNDRFVPMDA
                     GLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLVLNFLARP"
BASE COUNT         2852 a         3108 c         2845 g         2827 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9901 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9961 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
    10021 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
    10081 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
    10141 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
    10201 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
    10261 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
    10321 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10381 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10441 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10501 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10561 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10621 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10681 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10741 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10801 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10861 cgccgatgac ataccccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10921 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10981 tatacagttc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    11041 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    11101 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    11161 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    11221 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    11281 cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt
    11341 gctcaagagt atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca
    11401 attgattgaa agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat
    11461 gcagttcaga accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc
    11521 ggtcgttgcg cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa
    11581 aatccgggaa ctgtttcgga ttagcttcca ggctcttaac gaagttttcc ag
//
LOCUS       DAJTVJ010000131        11532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000131 DAJTVJ010000000
VERSION     DAJTVJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.131"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            460..963
                     /locus_tag="OO448_003464"
     CDS             460..963
                     /locus_tag="OO448_003464"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6338587.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKET"
     gene            complement(1336..2529)
                     /locus_tag="OO448_003465"
     CDS             complement(1336..2529)
                     /locus_tag="OO448_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6338588.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="OO448_003466"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="OO448_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6338589.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="OO448_003467"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="OO448_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6338590.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="OO448_003468"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="OO448_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6338591.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="OO448_003469"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="OO448_003469"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6338592.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="OO448_003470"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="OO448_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6338593.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="OO448_003471"
     CDS             complement(11165..11308)
                     /locus_tag="OO448_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338594.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2924 a         2896 c         2944 g         2768 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tagcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa at
//
LOCUS       DAJTVJ010000132        11427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000132 DAJTVJ010000000
VERSION     DAJTVJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.132"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="OO448_003472"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="OO448_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6338595.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="OO448_003473"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="OO448_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6338596.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OO448_003474"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OO448_003474"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6338597.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="OO448_003475"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="OO448_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6338598.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="OO448_003476"
     CDS             complement(3928..5214)
                     /locus_tag="OO448_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6338599.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="OO448_003477"
     CDS             complement(5235..6026)
                     /locus_tag="OO448_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6338600.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="OO448_003478"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="OO448_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6338601.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="OO448_003479"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="OO448_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6338602.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="OO448_003480"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="OO448_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6338603.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="OO448_003481"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="OO448_003481"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6338604.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="OO448_003482"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="OO448_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6338605.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="OO448_003483"
     CDS             complement(9882..10364)
                     /locus_tag="OO448_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6338606.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11427)
                     /gene="dgcN"
                     /locus_tag="OO448_003484"
     CDS             complement(10380..>11427)
                     /gene="dgcN"
                     /locus_tag="OO448_003484"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR6338607.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2802 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgct ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggcct tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtc
//
LOCUS       DAJTVJ010000133        11384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000133 DAJTVJ010000000
VERSION     DAJTVJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.133"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..187
                     /locus_tag="OO448_003485"
     CDS             <1..187
                     /locus_tag="OO448_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6338608.1"
                     /translation="RPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIW
                     DMGRIAMSVAQQPQCHK"
     gene            complement(455..766)
                     /locus_tag="OO448_003486"
                     /pseudo
     CDS             complement(455..766)
                     /locus_tag="OO448_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(785..2662)
                     /gene="bglF"
                     /locus_tag="OO448_003487"
     CDS             complement(785..2662)
                     /gene="bglF"
                     /locus_tag="OO448_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6338609.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2796..3632)
                     /gene="bglG"
                     /locus_tag="OO448_003488"
     CDS             complement(2796..3632)
                     /gene="bglG"
                     /locus_tag="OO448_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6338610.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3918..4643)
                     /gene="phoU"
                     /locus_tag="OO448_003489"
     CDS             complement(3918..4643)
                     /gene="phoU"
                     /locus_tag="OO448_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6338611.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4658..5431)
                     /gene="pstB"
                     /locus_tag="OO448_003490"
     CDS             complement(4658..5431)
                     /gene="pstB"
                     /locus_tag="OO448_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6338612.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5522..6412)
                     /gene="pstA"
                     /locus_tag="OO448_003491"
     CDS             complement(5522..6412)
                     /gene="pstA"
                     /locus_tag="OO448_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6338613.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6412..7371)
                     /gene="pstC"
                     /locus_tag="OO448_003492"
     CDS             complement(6412..7371)
                     /gene="pstC"
                     /locus_tag="OO448_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6338614.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7458..8498)
                     /gene="pstS"
                     /locus_tag="OO448_003493"
     CDS             complement(7458..8498)
                     /gene="pstS"
                     /locus_tag="OO448_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6338615.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8746..9819)
                     /locus_tag="OO448_003494"
     CDS             complement(8746..9819)
                     /locus_tag="OO448_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6338616.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9830..>11384)
                     /locus_tag="OO448_003495"
     CDS             complement(9830..>11384)
                     /locus_tag="OO448_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6338617.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2992 a         2933 c         2591 g         2868 t
ORIGIN      
        1 ccgccccgtc attgacagag catgggatgc tcaactcagg ctttgtaaga ggtatcgaaa
       61 acttcaggcc aaaggaaaga atgtcaatat tacaattgtt gctgttgcac gtgagctggc
      121 gggttttatc tgggatatgg gccgaatagc aatgtctgtc gcacaacaac cacaatgtca
      181 caaataaaaa atgagaataa ccagttccct gaaggcgacg tagcccggta caagagtaac
      241 ccacgttagc gcttggcgac ggcggaacaa agctgattcg cgacgtaaga aagtggcagg
      301 ctcatatgac ggacctctgt aatgcggtat ccaacccgcg aatatcagcg tgattcaccg
      361 tcggatttac tgctacgtcg ccctcaggga attaaaccta aaaagaaaat cgtcaaaatt
      421 agagattccg gtttgacacg gaaagtcata tcaacgctaa ccccgcttca ttcggttcgg
      481 cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga aatacgcaga caggtgaagc
      541 ccatctcggc aaataacgcg atatcttccg ggtaacggtg ataaaaatcg atggcgacat
      601 ctttgatatt ctctttcccc aggatgcgcg gttccatttt tcccataacg ccatgaggct
      661 gtaaatctga ggtcgtgctc cctttgccat cttccagcca ggcaccttcc acctgattgg
      721 cagctgttgc gccaccccaa agaaatgttt ctggaaatgc tttcataatt aactcctttt
      781 atcgttagcg aatgatggat aacagcggtt cacctgcgct tatctgcgcc gtgccgtggg
      841 gtaatacgtc tgtaaaatca tcgctattac tgattaatac cggcgtcgtc acatcaaatc
      901 cggcctcgcg aatagcaggg atatcaaaag aaatcagccg atcgcctgta ttgaccttgt
      961 cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag ttttacggtg tcgataccga
     1021 catgaatcag gatctccaca ccatcatctg actcaatgcc aatggcgtgt aatgtggcga
     1081 acaacgaagc aattcgaccc gcaaccggag aacgcacttc accaaccgag ggcagaatgg
     1141 cgataccttt acccaacagg ccactggcaa acgtggtatc agcgacgtga atgagcggca
     1201 caatctctcc cgtcatcggt gaacagatac cgccctgctc aggtggtgta ataacctctg
     1261 gtgttttctc ttgcggggca ccctgcgctg gctgacgttt agcggtgatg aaatgaagca
     1321 tcaccgtacc gacaaatgcg caaccgatgg caatgacacc gccaataacg ctggcccaga
     1381 cggtgaaatc aattcccgtt gacgggatgg tttgcatgaa ggtgaaaata cttggcaaac
     1441 caaaggagta cactttcgtt tgcgcgtagc caatgatggt ggcccccaaa gccccactga
     1501 tacaggcgat aacaaagggg tacttacgcg gcagattaac gccatatacc gctggttcgg
     1561 tgataccaaa caggcccgtc aacgccgctg atcccgccac cacttttttc tgcgcatcgc
     1621 gttcgcagag gaagacgccg agcgccgccc cgacctgcgc cataatggcg ggcattaaca
     1681 gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt gatacacagc ggcaccaggc
     1741 cccagtgcag tccgaacatg acgaagattt gccagaagcc gcccattacc gcgcccgcaa
     1801 atgcaggaac cgcctgataa agccagagat aaccggcggc aatcagttcg cttatccagg
     1861 ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat aaccatcaga catagcaatg
     1921 gtgtgaagaa atttttgatt gccgacggta accacgcatt aagtcggcgt tccagaatgc
     1981 tgcacaacca ggcagaaaaa ataatgggaa taaccgatga cgagtaattc aacaatgtga
     2041 ccggaatacc caggaaatcc agccccagcg catccgcttt ttgcccgttc tcgaaagcag
     2101 tcagaattaa tggatgcact aacgctccac caatcaccat ggcagtaaat ggattaccgc
     2161 caaagcgttt ccccgcggtg tatcccagga ttatcgggaa gaaccaaaac aaggcatcac
     2221 tggcgctgaa taaaattaaa taagtaccac tttgttcggt cgtccactga aaagtgagcg
     2281 ccagagccag catacctttc aggatcccgg ttgccgccat caaaccgatc agaggcgtaa
     2341 aaatacctga aataacataa acaaagcggt ttagcagatt acctttctca tcattttccg
     2401 gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt gaccgccagg aagacatcgg
     2461 ccacatggtt acctatgacc acctgaaact ggccaccgct ttccaccacc ataataatac
     2521 cgggggtgtt tttcagtacc tcagcttgcg ctttgctttc atcctttaac ttaaaacgta
     2581 atcgcgttgc gcaatgcatc agactaacaa tgttatctgc gcccccgact cctgcgacta
     2641 tttttctggc taactccgtc ataacttgcc ctctaccgct ttgcggcaaa actccaaaaa
     2701 aaaaactgaa aaaaacggcc tgacgtgaat caagcaattt ttttcaggtt ttgcccgctt
     2761 agtgcggtaa caatccttta ctcagtaata atatttcagt gttctttgcg cacgcgctct
     2821 atatttatgg ctaaaaacat aatctctgcg ggtgaaattt tacgttgata ctgcaaacca
     2881 ataaaaatgg cgatccgttc cgcacattgc catgcttgcg ggtaattttg ttttactgct
     2941 tgttgtaatg attcatcact atcgttaatt gaagcatgtt caagaatacg ccaggataaa
     3001 aacttcagat gtgtaaccag tcgctgataa ctcaagcttt cttcctggta attaaggctg
     3061 aactgaaatt ttattaattg cagcatttcg cgcattaact gcgtgacacc tgcaacatcc
     3121 tccatatttc cgctcatttg ggcactgacc agatgcatgg caataaagcc cacttcatct
     3181 ttcggtaact gcacgcccaa ccgtttatca ataatggtta acgcttcttc ccctagctgg
     3241 aactctttcg ggtaaagccg ctggatatcc cacagcaacg ggttgggcag caacacgttt
     3301 tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta gcgagatata aatactgtcc
     3361 tgtaattttc ccaagcgctc ctgcgctaaa gagataatac gatcacaggt tgccatcacc
     3421 tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt tcagttcatg actgctcaag
     3481 gcatactctt tttctattcc acttgagtta attctttcgc cagcgcattt ttgaaagcca
     3541 attcctcgcc ccatgacgac tttttcccgc tgttgatcat caataaccac cacaacatta
     3601 ttgttgagaa ttttggtgat ttgcatgttc atagcaagga ccttttttat aaacaaaaaa
     3661 aaccgacttc accagtattc tctggttatg tcaggttttg cctgcgaatg cagtaacaat
     3721 ccagtcattt attaatgttt tttataacga acatccaggt tcggaaatta atttaattgc
     3781 gtgcagagaa aatagcaatg cgctattgat aaaaatatga ccatgctcgc agttattaac
     3841 tttgtgtaat tttaggaatt tataaagtta tatataacaa atcccaataa ttaagttatt
     3901 gggatttgtc tggtgaatta tttgtcgcta tcttttcccg ccagcagttt atccagctca
     3961 tcaccgccta catgacggaa atcctgcccc ttcacgtagt agaagataaa ctcacaaata
     4021 ttctggcagc ggtcgccaat acgttcgata gaacgcgcgc agaacagcgc agtaagtacg
     4081 ctcggaatgg tacgcgaatc ttccatcatg taggtcatca gttgacgaac aataccttcg
     4141 tattcctgat cgactttttt atcttcacga taaatacgta ccgcttcgtc aatgtccatc
     4201 cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg tatgacggcc cagcgactcc
     4261 agacttacca gcaacggctg atgctgctgg gagaatttct ccagcgcagt acggcagatt
     4321 ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg ttttactgat caccataacc
     4381 agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg
     4441 atcgccactt ccatcatgtt gacgttcttg tcgccttcga tcacgcgctt cgccagatcg
     4501 ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct gctgctccac catgccaccc
     4561 atggtcatca cctgcgtgcg gatactttcc agttcggcgt tgaactggcc ggaaatatgt
     4621 ttattaagat tgagactgtc cataacgcac tcctgaatta accgtaacga ccggtgatgt
     4681 agtcttctgt ttgtttcttc gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa
     4741 tcaattcgcc caggtacata aacgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt
     4801 tgtgggtaac gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac
     4861 gcccggtaga gatggggtcg agcgccgaac acggttcgtc gagcagcagc acttccgggc
     4921 gaatggcgat accacgcgca atacacagac gctgttgctg accaccagag agagagtaac
     4981 cgctctggtg caatttatct ttggtttcgt tccacaatgc ggctttggtc aatgcccact
     5041 gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa cagacgaaca ccaaaagcga
     5101 tgttgtcgta gatggacatc gggaacggcg tcggtttctg gaacaccatg cccactttcg
     5161 cacgcagcag tgcgatatcc tgagagttgg tcaggatgtt gtcgccatca agcagaattt
     5221 caccttctgc gcgctgctcc ggatacagtt caaacatttt gttgaaggta cgcagcagcg
     5281 tcgatttacc gcagccggac ggcccgataa acgccgttac ctggttttta gcgatatcca
     5341 ggttgatgtt tttcagggca tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa
     5401 ttttactcgg ggcagtttca accatactca ttgcaatctt ttcctcatcg tacctgatgc
     5461 ttttcgctaa tcaggcttac cgtaggccgg gtaaggcgtt cacgccgcat ccggcaaagt
     5521 gttaaccgtg tttattcttc gcaaaaacaa cgcgcgccag aatgttcagc agcaggacgc
     5581 acagggtaat gatcaatacc ccggcccagg ccagttgttg ccattccgca aacgggctca
     5641 tcgcaaactt aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc
     5701 agaactggtt ggagagcgcg gtaaacagca gcggcgcggt ttcaccggca atacgggcaa
     5761 tcgccagcag gataccggtc ataatcccgg acaccgacgc tttcagcgta atcgcggaga
     5821 tcatcttcca cttcggtgta cccagcgcat aagccgcttc acgcaggctg tccggcacca
     5881 gtttcagcat gttctcggtg gtgcggataa cgatcggcac ctgcaacagc gccagcgcaa
     5941 ttacgcccgc ccagccggag aagtgctcca tctgtgccac cacgatggtg tagacaaaca
     6001 gaccaaccac aatcgacggc acggagagca gaatgtcgtt aatgaagcga atcacttctg
     6061 ccagccagga tttacgacca tattccgcca gataaatccc cgccataatg cccagcggcg
     6121 taccgaatac cgtggcccac aaaattaaca gcccgctacc cgccagggcg ttcgccagac
     6181 caccaccttc cgtattgggc ggcggcgtca tttcagtgaa cagcgccagc gacataccgt
     6241 cgataccgcg agtgatggtg gacattaaaa tccagatcag ccagaacagg ccgaaggcca
     6301 tcgtcgccat cgagagcgtc agcgcaatac ggtttttgag gcggcgacgc gcctgcattt
     6361 tgcggcgaga ttcagccagc gccgcagtgg tttgcatttc aaccatagcc attagcgtgc
     6421 cccctcattc ttagccaggc gcataatcat aaacttcgat gcggcgagga cgatgaaggt
     6481 aatcacaaac aggatcaggc ccagttccat cagtgcggca acgtgcagac cggattccgc
     6541 ttccgcaaat tcgttcgcca acgcagaggt gatactattg cccggcatgt acagcgaggc
     6601 gctgtcgagc tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccgagcgc
     6661 gcggcccagc cccagcatga tgccgccgat aacaccattt ttggtgaacg gaagaacgat
     6721 acgccagata acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac
     6781 cggggtttgt tcgaacacat cacgcattac cgccgcaatg tacggaataa tcatgatggc
     6841 gaggatcacg cctgccgcga ggataccgat accaaatgcg gggccagaga acagcgcgcc
     6901 aacaatcggg atattcgaca tgatattgcc gaccggctcc tgaaagtaaa cggcgaacag
     6961 cggcgcaaag ataaacaggc cccacatgcc gtaaacgata cttggaatgg ctgccagcag
     7021 ctcaatggcg atacccagcg ggcgtttcag ccagccaggc gcaagctcag tcaggaacag
     7081 ggcgataccg aaactcaccg ggacggcgat cagcagcgcg ataaacgaag tcaccaacgt
     7141 accgtagatc ggcaccagcg ccccgtagat atcgttcggt gcatcccact ctttggtcca
     7201 taggaaagcc agaccaaatt tctgaatgct cggccaggag gagatgatca gagagacaat
     7261 aatgccaccc aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat
     7321 tatgtcgccc tttttacccg gtgggttaaa agcaggcttg gttgcagcca taagttactc
     7381 ttcagttaaa cgcgtttaca aagtcatttt gtaatgccgg atgcggcgta aaacaccgta
     7441 cccggcctgg agttttatta gtacagcggc ttaccgctac tgtctttaat attggtcttc
     7501 cacgcagcgc gaacctgttc aactacacta tccggcaggc tggcgtaatc caggtcgttc
     7561 gcctgtttag ccccggtttt gtacgcccag tcgaagaatt tcagcacttc tgtgccttgt
     7621 tctggtttct tctgatcttt gtggatcaga atgaacgtgg tagaggtaat aggccatgca
     7681 tcttcgcctt tctggttggt cagatcctga gcgaaggttt tgctccagtc tgcacctttt
     7741 gctgcattag cgaagttttc ttcggtcgga ctaaccggtt taccatcagc ggagatcagt
     7801 ttggtgtacg ccaggttgtt ctgcttcgcg taagcatatt caacataacc aattgcaccc
     7861 ggcagacgct gaacgaacgc ggcgataccg tcgttacctt taccgcccag accgatcggc
     7921 cattttacgg tagagccagt accaacgttg tttttccact cttcgttcac tttcgccagg
     7981 tagctggtga agacgaaggt agtcccggag ccatctgcgc ggcgtactac agcaatgttt
     8041 tgtgaaggca gtttcagacc cggattcagt ttggcgatgg tttcatcatc ccacttcttg
     8101 attttgccca ggtagatgtc gccgagggtt ttaccatcca gcaccagttc gccagacttc
     8161 agccctggaa tgttaaccgc cagcaccacg ccgccaatca cggtcgggaa ctggaacaga
     8221 ccttcctgag ccagtttttc gtcagacagc ggcgagtcag aggcaccaaa atcaacggta
     8281 ttagcgataa tctgttttac gccaccggaa gaaccgatac cctggtagtt aactttatta
     8341 ccgatttctt tctggtaagt gtcagcccat ttggcataca ccggcgcagg gaaggttgca
     8401 cctgcacctg tcaggcttgc ttctgcaaac acagagaaag cgctcatcga taaggtcgcg
     8461 gcgacaacag ttgcgacggt ggtacgcata actttcataa tgtctcctgg gaggattcat
     8521 aaagcattgt ttgttggcta cgagaagcaa aataggacaa acaggtgaca gttatatgta
     8581 aggaatatga catttttatg acagaaagga attaacaaat aacttactga ttttaaatgt
     8641 gaagatgaaa atcccacatg tcatcttttt gtcatgatgc atttaaaagt gataaagaaa
     8701 tgggtgagat gacaacgatg tgatgaacaa tcgttgtcag acctattagc gcacaatctc
     8761 aacagttatc gtcgctgttg cagtaaattg ccccggggct gggcgagctc cagtaaggct
     8821 cgcaggagca gctgaaaaag ttacactacc tgatttttca ccatacactg ttgatttacc
     8881 catgtctgtt ggcaactcac caccgttcac tttgatttcc ttattatttt tgtcgtaaat
     8941 ttttatcccc acgttagagt ttgaagttac gaccattgaa ttgtccgtat tgggttcggt
     9001 agcataaaaa gacgtactga tatcatcggt attcagtatc cccaggtttt cacactcaat
     9061 agtgactgtt ttagtaattt tatgtgttgt taccgcactt ccaacaacag atgaaaaatc
     9121 tgcagctggt atttctttaa aatcaaaata aactggctgt aaattattta tttcgcaact
     9181 ttgcggagct gtaatatcgc cccgaatata aacatcagcc atcggctgtg agtaatccac
     9241 cggggtgtca cggctaattg ttccatacaa tgtcactatc tttgtttcag gaataatcaa
     9301 ttttcctgca aatgtcttct tgatataaaa atatactatc gctttacccc cactggatat
     9361 acttaaaggt tcaatgcggt tgcaattgta ctttgtatta ccagacgggt tgttagcatg
     9421 atattcaaaa ggtgcattca catacccaac cccaagaata tctatagcaa tcccaacatc
     9481 aaggttcgga ttaagatcat aataattaat attagagtga accccagcat aagttaatgc
     9541 ctggtttatt tttgacgtat aatagatata ggggaattct cctacattac cagtatcaca
     9601 atgacagcgt ccaggatatg aggatccatt agttacagtg gtattaaatg tcgttccaac
     9661 cttattttcg ttcgcggtaa taagatatcc cgagaaatca agcgtacggt gaaatacgcc
     9721 attatcaggg gtacagatac ctttaggagc tgcaagtgct acactactta acgagagcat
     9781 aaacacagca aagcaccact gtttgatata cttgttcata aaaatccttt taatgacatg
     9841 tcacaccctg ttcaattaat ccgctctcac tttctggtgt cgaaagtgaa taattgaaga
     9901 tgcattttgt tgaggcgttt tctccccagg ataacgtaac ctgtcctttt tctggtagac
     9961 cagaaagata aagttcgcca cggtcaccga caattcctga atctgccaaa cttgcatctt
    10021 gtatcgcagc aatggctcca aaaggtaccg gtttaccatt tgcatttaac actctgaaga
    10081 gaacgcgata acccacatga gtcacaaact ccgctttaac cactgcacca cgcgtgggaa
    10141 ccatcgtaac gacggtatta tcaagttcta tattcgactt cgagaaatag ttatcgctga
    10201 ttgctacccg attttcatca taagggttta gctgtgtctt gaccgtatag ccgcgccagt
    10261 ccgtcgcagc accgcgcata ttatctattt ctaacccggc ggccccaggt gctttaacaa
    10321 gagcaatagt ttcacctaac tcctgactta acgttacccc ttcgctgtgt gccagaattc
    10381 cccctctgat accataattc agcctctggg aattttggct gtagttatag ctgccgttca
    10441 ccgtgccata agagccatca taggtcaggg acatattacc gccaacctca tcatcatgac
    10501 tgttatagga ctgcattaaa ttccatgaaa ggttatttct ttccagcgcc gtaccgctga
    10561 gtccgagttg atgtgagcta ccggaattat cgttttgcga aacagaatag gtggcgtaac
    10621 tattacccca ccagcccaat aaaggaacag ataaagagag catgtagatc tgctcactgg
    10681 tgttatcact ggcatggttg ttccccgtcc cctgtctggc agtattggaa taactccagg
    10741 agacgttcca cgagagacgc ttccagctac tgctgtagcc aaattgcatt aatcgttcga
    10801 catcatcggt atgccagtat tgttgccggc ttaatgtcag gtataaattg ccataatctc
    10861 tccccaacga ctgcgttagc gtcccctcca cgcaactacg tcggttacct gtttcccaaa
    10921 agtcttcagg gctgttgcgc cgcgatgccc actcattgag ggtatagtag ccctcggtgg
    10981 agtaacgata gccaaccaag cgcaagctag tgtctgtgtc gtcaaatcgt tttgaataga
    11041 gaaagcgata tgactgaccc gtttctgtat catcatggct aaaattcgca cgagcatgtg
    11101 tcacatcgaa agaaattgcg cccaaagcgc caagatcttt accgacaccc agagctgcag
    11161 aaccataatt ttcagcagcc tgcatacctc catataaagt gatcccgtgg gaaaaaccat
    11221 gaagtatttg cgcctgaagg acgtccggtg taaatccgtc ttcatcgcgc aattcaccca
    11281 cgctgacatc aacatctgtc tgaccttcac gtttaagaat cgctaatgaa gcataaggtt
    11341 gggtgaatcg ttgttcgcta ccgtcttctt ctttgatggt tacg
//
LOCUS       DAJTVJ010000134        11357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000134 DAJTVJ010000000
VERSION     DAJTVJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.134"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OO448_003496"
     CDS             complement(<1..537)
                     /gene="espL1"
                     /locus_tag="OO448_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR6338618.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYA"
     gene            1062..2990
                     /gene="thrS"
                     /locus_tag="OO448_003497"
     CDS             1062..2990
                     /gene="thrS"
                     /locus_tag="OO448_003497"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6338619.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2994..3536
                     /gene="infC"
                     /locus_tag="OO448_003498"
     CDS             2994..3536
                     /gene="infC"
                     /locus_tag="OO448_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6338620.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3633..3830
                     /gene="rpmI"
                     /locus_tag="OO448_003499"
     CDS             3633..3830
                     /gene="rpmI"
                     /locus_tag="OO448_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6338621.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3883..4239
                     /gene="rplT"
                     /locus_tag="OO448_003500"
     CDS             3883..4239
                     /gene="rplT"
                     /locus_tag="OO448_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6338622.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4356..4480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4362..4406
                     /gene="pheM"
                     /locus_tag="OO448_003501"
     CDS             4362..4406
                     /gene="pheM"
                     /locus_tag="OO448_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6338623.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4689..5672
                     /gene="pheS"
                     /locus_tag="OO448_003502"
     CDS             4689..5672
                     /gene="pheS"
                     /locus_tag="OO448_003502"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6338624.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5687..8074
                     /gene="pheT"
                     /locus_tag="OO448_003503"
     CDS             5687..8074
                     /gene="pheT"
                     /locus_tag="OO448_003503"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6338625.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8079..8378
                     /gene="ihfA"
                     /locus_tag="OO448_003504"
     CDS             8079..8378
                     /gene="ihfA"
                     /locus_tag="OO448_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6338626.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8479..9459
                     /gene="btuC"
                     /locus_tag="OO448_003505"
     CDS             8479..9459
                     /gene="btuC"
                     /locus_tag="OO448_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6338627.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9522..10073
                     /gene="btuE"
                     /locus_tag="OO448_003506"
     CDS             9522..10073
                     /gene="btuE"
                     /locus_tag="OO448_003506"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6338628.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10073..10822
                     /gene="btuD"
                     /locus_tag="OO448_003507"
     CDS             10073..10822
                     /gene="btuD"
                     /locus_tag="OO448_003507"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6338629.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10900..>11357
                     /locus_tag="OO448_003508"
     CDS             10900..>11357
                     /locus_tag="OO448_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6338630.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2872 a         2631 c         3058 g         2796 t
ORIGIN      
        1 ggcgtattgc aatgacagat ggcgacaaaa aatagcgtca gaaggagatt gcgaaaaaaa
       61 tgcactacca ttgagattta ctgttggttg tatagttttt tctctatgga tttttttaga
      121 cagtttcata caatggttac gatcttcggc agttaactgt tcctgttcaa tataatccag
      181 tatgcttcgc aaatggttag ccttaatagg cgtggtttcc agaaataagg gaatgatttc
      241 ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg atgggtgaag tggcaaagtt
      301 aatgataaaa agggcagtgg aataacaatt ttgtgaaatt tttcgacagt ttgcggtaaa
      361 ttcctgagta acacagtatg acagcatcga acttaagtca gaggtatcaa gtagggtgat
      421 gagttggcgc gcaaattgat taataggctc tgatgaaggc gttggtatgt cgctatcgat
      481 gtgcaatcta tgaggagact tataatgggt tctgataatg atatcgtttt gactcataat
      541 ctattcgcct tatatatgtc tggaggtttt gaggatgatg attaattatt atcgccagaa
      601 cgaaatgtgc acgaaaaatt gctataagaa ttgtgtcact tttatcattg tgatatgacc
      661 acttcgtcat taatctcatg agcgtttaaa atactaccgc caaatcacgc aacgaacttt
      721 gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg
      781 atgttatcag tggttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat
      841 cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgttcaac
      901 cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgcga accaatttag
      961 catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt
     1021 gatctttcgt gtgggtcacc actgcaaatt aaggatataa aatgcctgtt ataactcttc
     1081 ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt gcgctggaca
     1141 ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg
     1201 cttgcgatct gattgaaaac gacgcacaac tgtcgatcat taccgccaaa gacgaagaag
     1261 gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcatgcgatt aaacaacttt
     1321 ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg
     1381 atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc
     1441 ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa
     1501 ctttcgccaa ccgtggggag agctacaaag tctccattct tgacgaaaac atcgcccatg
     1561 atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg
     1621 taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc
     1681 gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa
     1741 aagcacttaa cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa
     1801 tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc
     1861 acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag
     1921 agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa
     1981 ccggtcactg ggacaactac aaagatgcaa tgttcaccac atcttctgag aaccgtgaat
     2041 actgcattaa gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt
     2101 cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt aacgagccgt
     2161 caggttcgct gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct
     2221 tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata
     2281 tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac
     2341 gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag
     2401 aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg
     2461 aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact
     2521 tctccttgcc gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac
     2581 cggtaatgat tcaccgcgca attctggggt cgatggaacg tttcatcggt atcctgaccg
     2641 aagaattcgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt atcatgaata
     2701 ttaccgattc acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca
     2761 ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt
     2821 tgcgtcgcgt cccatatatg ttggtctgtg gtgataaaga ggtggaatca ggcaaagttg
     2881 ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga
     2941 agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag
     3001 gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg
     3061 aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc
     3121 tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc
     3181 cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg
     3241 aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctggtacag
     3301 atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata
     3361 aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg
     3421 aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa tccttcccaa
     3481 cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc
     3541 ttcaagtagc actgtctgtg gagccttcgg gcttcacagg ctttgttcgc ctttgtttcg
     3601 tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg
     3661 ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc
     3721 gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg
     3781 tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg
     3841 tttttaactt tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt
     3901 ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt
     3961 gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct
     4021 taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac
     4081 gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc
     4141 tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc
     4201 accgctctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta
     4261 gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc
     4321 aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc
     4381 gcttcttttt ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga
     4441 aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa
     4501 aaagccagtc tatcaggcgt aattgtcgca gccagtctgg acacggccag cgcggagaaa
     4561 ccggagcgta tttaagtacg tgagaatttc gagcactgcc cgggaccaaa atggcaagta
     4621 aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga
     4681 ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg
     4741 cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact
     4801 taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg
     4861 cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac
     4921 tggaaagcgc agcgctgaat gcgcgtctgg cggcggaaac gattgatgtc tccctgctag
     4981 gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa
     5041 gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc
     5101 ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt
     5161 tctggtttga cactacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca
     5221 tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgcgtttat cgtaacgact
     5281 acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca
     5341 tcagctttac caacctgaaa ggcactctgc acgatttcct gcgtaacttc tttgaggaag
     5401 atttgcagat tcgcttccgt ccttcctact tcccgttcac cgagccttct gcagaagttg
     5461 acgtcatggg taaaaacggt aaatggctgg aagtgctggg ttgcgggatg gtgcatccga
     5521 acgtgctgcg taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg
     5581 ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa
     5641 acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga
     5701 actgtggtta cgcgaatggg tgaacccggc gattgatagc gatgcgctgg cgaatcaaat
     5761 cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt
     5821 ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac
     5881 aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc tgtggtgcgc caaactgccg
     5941 tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat
     6001 taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tctctgaact
     6061 gggaatttct gacgatcaca gcggcattat cgaactgcct gcagatgcgc cgattggcac
     6121 tgacatccgc gaatacctga aactcgatga caacaccatc gaaatcagcg taacgccaaa
     6181 ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac gttgccgtgc tgaatcagct
     6241 gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc atcgacgaca cgttgccgat
     6301 tacagtcgaa gcgcctgaag cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa
     6361 cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc
     6421 tatcgatgca gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca
     6481 cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcaa aagagggcga
     6541 aacgctggtg ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc
     6601 cgaccacaac aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa
     6661 tgacgaaacg caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg
     6721 tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga
     6781 tccggcactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg
     6841 tggtgaggct ggtccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac
     6901 catcacttta cgtcgtagca aactggatcg cctgatcggc catcatattg ctgatgaaca
     6961 ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgagtggca
     7021 ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg ttgaagaagt
     7081 cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat
     7141 gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa
     7201 aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca
     7261 tccaggcgtt gaagccttac tgctgccaag cccgatctct gttgaaatgt cagcaatgcg
     7321 tctttctctg tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa
     7381 ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg
     7441 cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg
     7501 gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg aatccgttct
     7561 cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca
     7621 tccggggcaa tccgcagcga tttatctgaa aggggaacgt attggttttg ttggagttgt
     7681 tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga
     7741 gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc
     7801 gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc
     7861 cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg
     7921 cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac
     7981 cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt
     8041 aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa
     8101 tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg
     8161 aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg
     8221 gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg
     8281 gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa
     8341 aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg
     8401 ctctgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
     8461 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
     8521 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
     8581 gaacaatgga tttcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
     8641 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
     8701 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
     8761 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
     8821 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
     8881 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
     8941 atctgtagcg cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag
     9001 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
     9061 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
     9121 gcacttggcg agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg
     9181 ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
     9241 tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta
     9301 ttacttcccg gctgcgcgct ggcaggggcg agcgcagtgc tgctggccga tattgtagcg
     9361 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
     9421 ccggtgttta tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga
     9481 ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga
     9541 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg
     9601 tgctgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
     9661 agaatattca gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
     9721 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
     9781 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa cggcgaagga cgccatccgc
     9841 tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct
     9901 atgcccgtat ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt
     9961 ttgagaaatt tttagttggc agggacggat tggtcatcca gcgtttttcc ccggatatga
    10021 cgccggaaaa ccccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat
    10081 tgtgatgcag ttacaggatg ttgcggaatc tacccgccta gggccacttt ctggcgaggt
    10141 tcgggctggg gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact
    10201 ggcacgaatg gccggaatga ccagcggtaa gggaagtatt cagttcgcgg ggcaaccact
    10261 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac
    10321 gccgccgttt gcaatgccgg tctggcacta tctgacactg catcagcacg ataaaacgcg
    10381 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggacgtag
    10441 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca
    10501 aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag
    10561 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt gtcagcaagg
    10621 actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc
    10681 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc
    10741 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
    10801 aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct
    10861 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
    10921 atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
    10981 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
    11041 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
    11101 gtaacgatgc gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga acaagcctct
    11161 atcggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg
    11221 ggttccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac
    11281 gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat
    11341 ttctggcagg cgagacg
//
LOCUS       DAJTVJ010000135        11288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000135 DAJTVJ010000000
VERSION     DAJTVJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.135"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            781..1848
                     /gene="pilL"
                     /locus_tag="OO448_003509"
     CDS             781..1848
                     /gene="pilL"
                     /locus_tag="OO448_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HCR6338631.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAHPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1848..2285
                     /gene="pilM"
                     /locus_tag="OO448_003510"
     CDS             1848..2285
                     /gene="pilM"
                     /locus_tag="OO448_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HCR6338632.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            2299..3981
                     /locus_tag="OO448_003511"
     CDS             2299..3981
                     /locus_tag="OO448_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HCR6338633.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3974..5269
                     /gene="pilO2"
                     /locus_tag="OO448_003512"
     CDS             3974..5269
                     /gene="pilO2"
                     /locus_tag="OO448_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HCR6338634.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAASAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            5256..5708
                     /gene="pilP"
                     /locus_tag="OO448_003513"
     CDS             5256..5708
                     /gene="pilP"
                     /locus_tag="OO448_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HCR6338635.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5719..7272
                     /gene="tadA"
                     /locus_tag="OO448_003514"
     CDS             5719..7272
                     /gene="tadA"
                     /locus_tag="OO448_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR6338636.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            7285..8382
                     /locus_tag="OO448_003515"
     CDS             7285..8382
                     /locus_tag="OO448_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCR6338637.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            8400..9014
                     /locus_tag="OO448_003516"
     CDS             8400..9014
                     /locus_tag="OO448_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HCR6338638.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            9024..9584
                     /locus_tag="OO448_003517"
     CDS             9024..9584
                     /locus_tag="OO448_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6338639.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            9569..10225
                     /locus_tag="OO448_003518"
     CDS             9569..10225
                     /locus_tag="OO448_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6338640.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            10225..>11288
                     /gene="pilV"
                     /locus_tag="OO448_003519"
     CDS             10225..>11288
                     /gene="pilV"
                     /locus_tag="OO448_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HCR6338641.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITAGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGA"
BASE COUNT         3236 a         2669 c         2559 g         2824 t
ORIGIN      
        1 cgtctgcgtt tctccggtcc taagacatca atcatctgct ctccaatgac tagtctaaaa
       61 actagtatta agactatcac ttaaataagt gatattggtt gtctggagat tcagggggcc
      121 agtctactgt tatcttagtc gccggcatca actaacatga aaaataataa cgtaccatta
      181 tatctatggt tactttggtg tacgttttgc ctgtacatca ctaccccgtg gcaatacttc
      241 cgttatctat acactgacca catatcacta tcttatatat tatatttctg tatagtggtt
      301 attatctgct tttccccact acttttcaga tgcgtgcttc ttatcgtaag gtctgtatct
      361 aaatacaact tcacattctt tgcgaaaaaa aatggcagta aagaatcaag catacatctt
      421 agcccctatg ccgatacacg ttgcccggag tcaatgagga aacactggga tgtgttactt
      481 tctctgatat caaaagcaat taataatgat gaaaccattg ttatgcattc acatctcttt
      541 actcctgcaa gaataaataa acttataaag agactggaat cttatggttt gaatttctct
      601 gttattacat gcccacggaa aacaagtctg tatgaaagat tatcaattcc ttttgtatat
      661 ctcttatttc agtggtctct cccgcatttg cattcggacg gaatgacagt cgtcattcgt
      721 cctctccagc cgcctcacac caattaacaa acacgtatat acgaacacgg agtctccgtt
      781 atgaaaaaaa actatctgac acttatactg ccgtcattat tgtccggctg tgccggaatt
      841 tccggcagca ataacgtggc accatcagat acgcctgctc tggtttttgt tgacggacaa
      901 atcagtgaga gtgctggcgt tctggcactt acgcaacaac gtctttctcc acctaaaata
      961 actccccccc cgagagtcag cgtgccagag acagtaaaaa cagcacatcc agtcagcgaa
     1021 tcccgaccgt taccttcacc aacaataagt cagaatttct cccggttaat cattacaggc
     1081 aagcctggta gcgcccctgt aacactatcg ccgacggccc gtaaccggac cgtcagtcag
     1141 tggataaaaa cattactacc ggaaggatgg aaattcagac aagagaatgc agccacgccc
     1201 aagctgaata ccaaactggt ttcatggtct gccaatgatc agtggacccg ttctctgaat
     1261 cggttgctgg aagaacaaca cctatggggc catctggact gggataataa aacgttgacc
     1321 gtcacaacat cagctgctgc accggcacag gacacggcaa ccactatctc gccagcagcc
     1381 atggtaacca catccacttc ccctgaatct ccaacaacag caaatagcca gaacaaacca
     1441 cgaaatccat tcagaggaaa cagcgtatct tcgtcaaccc ccgccgccac cccgactcca
     1501 accggctcca cagtcaagtc tatcccgctc atgaccggaa cgcctgttaa accggtatct
     1561 cagggtaaag agtggcgtgc gccggcaggc accacattaa gagagaacat tatcaagtgg
     1621 gcagaagaaa caaaatgcga aagcatggca tcaacgcact ggatggtcat atggccactt
     1681 tctgtaaccg attaccgtct ggatgcgcct ttaatgttcc ggggatcatt tgaatcagcc
     1741 atggagcagg tatttgagtt atatcggact gcgcaaaaac ctttatacgc acaggcttct
     1801 cgcatgcagt gcatcattac agtttctgat acaagggatg gtcgctaatg ggatggctgg
     1861 tcatggccgt cggcctgacc attctgatta tcactggtag ctaccagaat cagaagatgt
     1921 ctgaaaccac aaacgcccag caatatgcaa gtgcgtctgt atgggcaagt cagatactga
     1981 tgattgctaa cagaatcaat gatatcagat acgtatcagg ccagcaggac ggagtaattt
     2041 cctcagataa actggctctc cctgtcactc cggactcccg aattaaacat cagttacaac
     2101 aggggcgtct gtgggtatgg atgcctgaac aaccaggtct tgttgaaact ttgcgttcaa
     2161 aaagtcgtgg ttctgcacta atagggatct tccaaaacgg gcaactcacc tggctatcag
     2221 gaactgcaac cggtctgaca cccccggcag gcattactgc tggttctgtc gtctacgtta
     2281 actaatctgg atatattcat gaaaaaatca caccagcgtt caatgaagct ggcggtgctc
     2341 ccctgcatga tcgccgtcgc tctttccatc tccggatgta ctttcagcga aatcaacaaa
     2401 atgcagaaaa aagcacagga agactcagca catgcacggg aaaaggtatc agccctttcg
     2461 gcccgtaaat cgcaggctct tacctggctc gataatcaat ggataaaccc tgttccggtc
     2521 gctcaggtat caagagagaa aaaacaaaca gctccggcct gctacatcac gcaggcaaga
     2581 aaaggagaga tcactctgca ggaactgggg caacgtatta ctgccgtatg cggcatccct
     2641 gtgatcatca cgcctgacgc agccaattca actcttgaag gaggcgctac ccgccagatg
     2701 acaggaacac taccagcacc agatgaaaat gggcgtctac cgttaagcag cctgggcagc
     2761 acaacaatga ctacctccac tcagccatta acgctgaata acctcatgtg gcagggagat
     2821 atcaatggtc ttctggatct gatggccagc cggagtggtc tgtactggcg catggataat
     2881 ggtcggattg tattctatct gactgaaacc agaacgtatc cacttcatat gctgaacacc
     2941 aaaaccagca gcagttccag tgtcagctct ggctcaacaa gcacaatggg ggcaacagga
     3001 ggccaggata actcagcatc cggtgatgca acgtcctctc agagcacaac cgttggtcag
     3061 gaatacgatc tgtatgaaga catccggaaa actattgaag caatgctgac accagaaaaa
     3121 ggccgttact ggttatctgc atcgagctca acgctgactg tcactgatac tccagctgtc
     3181 caggaagccg tcgcacgata tgtggacgaa caaaacagta ttatgaaccg ccaggtagcc
     3241 ctgaacgtac aggttctgag cgtcagcaat accagaaacg aacagttcgg tctggactgg
     3301 aaccttgttt ataaatcgct acattccgcc ggagcaacgt tgaacaatgc aagcggagat
     3361 tttacaggcg ctacatctgc aggcgtatca attctggata cggcaacagg gaatgccgcc
     3421 aaattcagcg gttccagtct tctgattaaa gcgctgagtg aacagggcga tgtcagtgtt
     3481 gtgacttcac aagaaagcac tgtcacaaac ctgacgccgg tacctatcca gatggcagat
     3541 cagacggttt acgtcgccca gtcagcaaca acaacgacta cggatgtagg agcaacaaca
     3601 acattaacgc cgggcatgat caccaccgga ttcaatatga ccctgctgcc tttaattcag
     3661 aaaacgggca atctccagtt gcagatgaat tttaatctgt cagatccccc aacaatccgt
     3721 agctttacgt caaaagacgg aaacagttac atcgaaatgc cgtataccaa actgcgttca
     3781 ctgagccaga aggtcaatct gaaagaaggg caatcacttg tcgttactgg tttcgatcag
     3841 aacaatacga cgacaagtaa agccggtacg tttacgccag caaatccatt atttggtggt
     3901 tcacaaaccg ggaaaaatga acgcagcacg cttgtaatca tcattacccc gactttcccg
     3961 tcaggaggca acaatggctg atgaagatat caacccggtt gtacttctcg cagatcctaa
     4021 agtaaatcat cgcgtgtggg cagcctgtct taaatggagt cctgtcgtaa aaaaacagcg
     4081 agtcccttct catcagaaac acaagcccca tgtaaaatcc agacggctta cctctctgaa
     4141 agtgactgta ggcagcagaa gttcccgtgg gaagatcagc cgcattaccg gcacaggaat
     4201 actggccagg ccggagcgca atcattattt ttcccttgct ctggctttct gctcatgggt
     4261 gagaaatgga tacggcgtct tccggtacag cgataaggag cttttattcc ttgcctccat
     4321 taatggccag cctgccgtta tggctgatct gtcaggaaat gatgctgatg ttgctcaaaa
     4381 ggtcagtctt tttctgacga tgaacgaaga gccccccgaa aaatggcagg tcgtatctcc
     4441 tctggaacat cctgataact gggaaagtat tattacccgg ttatcatcag cagatctccg
     4501 ccgttgcaaa cttactgtcg gcaatcgcag caaattcaca ctgcctgccg tacttttttt
     4561 agtagcagcc agcgcaggca ctgtattctg gatgactcag ccagagccgg atgtcgtacc
     4621 gacgcccgag gagatagctg cacgggcaag attgcagttt aaaaagcccg atccaccgcc
     4681 agaactcccg catccatggg catcacaacc tgtaatcagt gattttctga aagcctgcgc
     4741 ggaccttcga aaaccatcac cggtcgcgct agaaggctgg aaacttaccg gtggaacatg
     4801 tactcccgag acattcacac tgatttacga acgacagcca ggaggaacta ttgaaggctt
     4861 tctggcaaga agcaaagaaa tctttaatgt tattcctgac tttaacctta aagatggtgc
     4921 acgcctggct tctgtaaccc ggccactgcc ttccctgccc cgcagggatg aggcagtccc
     4981 taccccctca gaacaactga tgcgggtgtt cacgtggttt cagaaaaaac agttaacgcc
     5041 ggccattaat gaaatagcca taccggaacc attaccaggt aacgatggtg aaccagcccc
     5101 tgttcagaaa tggaaagaat atcagttcag tttatcaacg cctgtaaatc ctgatgagtt
     5161 gttcccttta tttcaggata ccggcgttcg tctgagcaat atccattttg aattaaacgg
     5221 cggtactttc agttattcct cagaaggtca tatttatgcg tccaggtaaa ttactcatta
     5281 ttccatccat atttttcttc tccggttttt catttgcaac aacacaacct cttgtcacaa
     5341 taggagaact tgaagcacaa caaaaccgga atattctttt acaggcaaaa gttcagggcg
     5401 cacagctaca gaaacaactc gaagaatcag atgttacttc atcatctgaa actgtttcag
     5461 gtttctctgg tgtgactgcc tctttgccat cagtttcaga gcagccgaca tctaaaaaca
     5521 gaaaggaatt gcccgttatt atggaaataa acggcaaaga taaacgtctt aatgccgttc
     5581 ttcgcatggc tgatggacgt cagacaagcg tcaccactgg cagccagctt ccaggcacat
     5641 ccgtcacagt gaagtcgatc tcattgtctg gcgtcacgtt aagtgatggc accaccctta
     5701 ctttctgagt aacgtgatat ggataaatat aatctgaagg atgctctcct ttttatatcc
     5761 aggggagaca cccatgaaat tcttatagaa acaaaccagc ggacaaggcc tgacgtacaa
     5821 agtaatctcc aggagctgct aaaattatat ccggatatca acccaaaagt agtctcgttg
     5881 tcggagttac aggaggcagg tcaggatgag aaaaacagag gcccgaaaga acgaattatt
     5941 catctgaaag accttgctga tgtcagtgaa agtgagaaga aagttctatc ttattttgaa
     6001 actgccagaa aacttggtgc ttcagatatt catttcttaa tttcagagtc tattttcaaa
     6061 gttcgcatga gaatattcgg tgaactgcag actgtagatg aagatcaacc agcactgggc
     6121 tattcacttt gcgcaacagc cattctgtca atggctgatg tcactgaaac cagtttcttc
     6181 cctcaaaggg agcaggatgc gcgcctgtcc ccacaattaa tgcgaaaaat aggaatattt
     6241 ggtgcacgct acagtcacag acctacggga gacggtctga ttgcagttat gcgcctgata
     6301 cccgatgacg gagataaagt tcccacgttt aaacaactgg gctttatccc ggaacaaatc
     6361 cggttgctga acataatgct gcgccgcccg gaaggaaaaa tagtcctgtc aggtccaacc
     6421 ggttccggta aatcgacaac gttacgcagc gcctgccgtg tatatcttga cgataatcag
     6481 gggcgacacc tgctaacaat cgaagatccg ctggaaggac agatactggg agcaacacag
     6541 actccgatta tctgcgacaa atccgacgaa gatgccgtca aactggcatg gagtcgggct
     6601 atttcatcgg caatgcgact tgaccctgat gccatcatgg aaggggaaat gcgcgatctt
     6661 atctcgatga tgtcaaccac ctacgcggca cagaccgggc acatagttct gacgacatta
     6721 catacaaact cagctctggg tataccggag cgaatgatta cgatgggtat gaatgccgat
     6781 cttatttgcg atgcgcaatt actgattggg atgatcagcc agcgccttgt accaactctg
     6841 tgtccctcct gccgtattcc atgggaaaag cgagcacctg aacttagcga cgacgagcgt
     6901 gattaccttg agcggcactg caataaagat tcactctgca gtacagataa tatctggttc
     6961 cgaaatcatc acggatgctc tgaatgtaat cacgacgtaa tcattaacgg cagaaagcgc
     7021 ggtgaaatag gtaaaggcct caccggcagg actgtaattg ctgaggtgat agaacctgat
     7081 aaccgcttat ttcagattct gaaaacacgc ggcaaagttg cagcacgtaa atactggctg
     7141 gaaaacatga aaggcatcag tcgtgtagag catcttcttc gccgtatcaa tgaaggtctg
     7201 gtagaccctc ttgaagccga cagaatcatc cctctggacg aagacgagcg cctcagcatc
     7261 gatgatgtct aacaggagaa caacatgcgt gaaatgaatt tcagtcaacg gctgaggcgt
     7321 ttcattgtca gaaaaacgtt cagcgccccc tacagggtgc agttttacga agccctgcgt
     7381 tttctgctgg aaaataaaca gccattaaag accgcactgg aacagatgcg ggatgcctgg
     7441 acagactttg gacgaaaatg gcatcccttt gctgaactgg ccacagactg cattgagtct
     7501 cttcgtgaaa acagtggcga aaactctctg gaatatactc ttagtttatg ggttcctcag
     7561 gaagaggccg cagtaatcag cgccgggata agaagtggtt caattgtgga tgcactgcaa
     7621 ttcgccacca ctcttacaga tgcaaaagaa caaatccatc aggctatatg gcaaatggct
     7681 atttatccgg tgggattgtt aatcatgatg acggggacat tatatgttct gaatacagag
     7741 ctaatacctg aactgagcaa aataagctcc cccgattcat ggagtggtgc tcttggtttt
     7801 ctttatggat tatctgtttt tgtcgataat tacggcgcta tatgtgccgt tctttttgca
     7861 gtgatcacag gtttaatatc atggtctctg ccaaactgga aaagccctga cagtgttcgt
     7921 acttttgccg acaagataat gccatggagt atttatcagg atatacaggg agcaacattt
     7981 ctcctgaata tggctgcact tctgaaagcc aaaatgacga cgctcaactc gctaaacatt
     8041 cttcaggagt ttgcatcacc atggttaagc acacgtcttg acagcattat ttaccgcgtc
     8101 cgccaggggg atcatcttgg actggctttg cgtcagtgcg gttatcagtt cccatccagg
     8161 gaagcagcca acttcctgtc tttattacag ggagatggcg caacagagct aatcagcaat
     8221 tatggtcaac gatggcttag tcaaacactt caacgtgtaa agaaacgggc aaacgtgatt
     8281 cgtttaatta tgctgatatt tcttgtcatg tctctcatgt tactggtttt tgccattatg
     8341 gatattcagt ctattagtga caacagcatg ggtaactttt aaacaaaacg aggatattta
     8401 tgttagtaga aaatattaat accactttaa ctggaaataa taagaagaat gagcctcatg
     8461 ataaaggatg ggcaattttg gagcagggaa caatagcgct cgttgttctt tttgttatag
     8521 tcgttgttct gggtagtttg tatgcgttgc gtacaagagc caatgtggca actgaaaccg
     8581 caaatataca aacaattatc acaagtgccc agagcctgct aaaaggtagc gatggatata
     8641 catttaccag cagtgccaaa atgacaggtg ccctcattca gatgggtgtc attccctctg
     8701 ggatgaccgt acagggcgac aaaacatctg gtacagcaac gctctataac gcatggggcg
     8761 gtgctgtaac tgttgccccg gcttcaacat cagggttcaa caacggtttt accgttacct
     8821 atgataaagt cccacaggat gcctgtatcc agattgccac acgaatcagt aaaactggtt
     8881 taactaacgg cattaccctg aacagtactg ctcacagtga tggaaaagtc actactgaag
     8941 aagccagtac gcaatgcaag gcagataatg gcagcactgg tacaaacaaa cttatcttca
     9001 ccatcaatgg ttaaggagtt ttaatgtcag ggggtacagt acccctcaga cttatttcat
     9061 taatcctgct tttttctctt attccacccg cccgagcttc atacgtagct cccaaacaga
     9121 tctggaccaa caagtgggac aattgctttg ccgctgccgg cgcgcgctat caaattgaac
     9181 cattattatt gaaggctatt tctgccgggg aatcttcgtt gaggccgggc gcaattaata
     9241 taaataagga caaaaaaaca ggtaaagcca gtagcacgga ttatgggttg atgcaaataa
     9301 actcaaccca tattccaaag ctgataaata tgggcgtaat aaaaaagtct gaggatttaa
     9361 ttaccaaacc atgccttaat atccatatag gaagctggat actggcaaga cattttcaga
     9421 tatgtggcgt cagctggaat tgccttgggt catacaatgc cggattccgt aaggacagac
     9481 atgaaacaag agagcaatat gccaataaaa tatggagaat ttatcgtgac atgaaaggga
     9541 tatgtcttcc cggacaggga ggcaggcaat gcagacaatc gtaggcataa gtctatcccc
     9601 tgtttatata ctgccattga tgttcacatt ttccattatt ggacgaactc tgctattcag
     9661 agttcgttat tttttatctg atacgggaca tctctggtat aagacacacc ctgctgttct
     9721 gtcaggaata tggctataca gtatagctgt ggcgttaatt attttatcat catcaccact
     9781 gctctaccgt attcatgctg tccttattct ttcgttcata ctgcaaatgg cggtaacgga
     9841 tgcactgaca ggtttattac caggaacatt cacccgacgt tttttaattg caggaatgct
     9901 atcacaaatc acgactgata tatggtggtt ccgtacaaca gaatttgcca ctgctgcaat
     9961 tgtcttattc tgccttcata aattagttaa ccgccatcgt cttaatattg gcactggaga
    10021 cctgtggctt attgcaggta ttaccgcatg gtcagggtta tataacgcca tatggtgtgt
    10081 gttgctgggc acaggaggat ttgtcttatg gcattcaaca tggtgcataa aagggcataa
    10141 agaaggccct ctgggtccat ggctatgttt tggccatgtc cttttgctgc tggataatct
    10201 ttatcaacca ctatgggtaa tctgatgaaa aaatatgacc gaggctgggc atcgctggaa
    10261 actggcgcgg ctttactgat tgttatgctc ctgattgcct ggggagcggg catatggcag
    10321 gactatattc aaacaaaagg atggcaaact gaagcacgtc ttgtcagcaa ctggaccagt
    10381 gcggcccgct cttatatagg gaaaaactac acgactctcc agggcagtag taccacaaca
    10441 actcctgccg ttatcacgac aaccatgctg aaaaataccg gctttttgtc cagcgggttt
    10501 actgagacaa acagcgaggg gcagcggtta caggcatatg tggttcgaaa cgcccaaaac
    10561 ccggaattac tacaggcaat ggttgtatcc agtggtggca caccttatcc agtgaaagca
    10621 cttatccaga tggctaagga tattaccgct ggtcttggtg gatatatcca ggacggcaaa
    10681 acagccacag gtgcattacg ttcctggtca gtagctttaa gtaattatgg tgccaaaagc
    10741 ggtaacgggc atattgccgt attgttatcg acagatgaac ttagtggtgc agctgaggac
    10801 actgatcgtc tttacagatt ccaggtcaat ggtcgccctg acttaaacaa aatgcacacg
    10861 gccattgata tgggatcaaa taacctgaat aacgttgggg cagtaaatgc ccagacaggt
    10921 aatttcagcg gcaatgtgaa tggtgtaaat ggcactttca gcggtcaggt aaaaggcaat
    10981 agcggaaact ttgacgtaaa tgtcaccgct ggcggtgata tcagaagtaa taatggttgg
    11041 ttaattactc gtaacagtaa aggctggctc aatgaaactc acggtggcgg attttatatg
    11101 tccgatggat catgggttcg aagtgtaaac aacaagggca tctataccgg cggtcaggtg
    11161 aaaggcggta ctgttcgggc tgatggtcgc ctttatactg gtgaatactt acaactggaa
    11221 agaactgccg ttgctggcgc atcatgttcg cctaacggcc ttgtaggccg cgataataca
    11281 ggggcaat
//
LOCUS       DAJTVJ010000136        11065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000136 DAJTVJ010000000
VERSION     DAJTVJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.136"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..309
                     /locus_tag="OO448_003520"
     CDS             <1..309
                     /locus_tag="OO448_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6338642.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OO448_003521"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OO448_003521"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6338643.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO448_003522"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO448_003522"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6338644.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="OO448_003523"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="OO448_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6338645.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OO448_003524"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OO448_003524"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6338646.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OO448_003525"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OO448_003525"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6338647.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OO448_003526"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OO448_003526"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6338648.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OO448_003527"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OO448_003527"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6338649.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /locus_tag="OO448_003528"
     CDS             complement(8117..9763)
                     /locus_tag="OO448_003528"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6338650.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /locus_tag="OO448_003529"
     CDS             complement(9766..9816)
                     /locus_tag="OO448_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338651.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OO448_003530"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OO448_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6338652.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..11065
                     /locus_tag="OO448_003531"
                     /pseudo
     CDS             10354..11065
                     /locus_tag="OO448_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2509 a         3049 c         2896 g         2611 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gccccctgat cactgttgag gtacatatca gtaaaggtct
    10441 acccggctta acgatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt
    10501 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10561 tgctccagcc gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10621 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga
    10681 actggctctt acaggcgctc tgcgtggcgt tcccggtgca atctccagtg caactgaagc
    10741 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10801 taacggtgaa ggatgcctga tagccgatca tctgcaagct gtctgtgcgt ttctggaagg
    10861 taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga
    10921 tctcagtgat gttgtcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg
    10981 caggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    11041 tattaatggc ctgttgccag attta
//
LOCUS       DAJTVJ010000137        11016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000137 DAJTVJ010000000
VERSION     DAJTVJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.137"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            24..236
                     /locus_tag="OO448_003532"
                     /pseudo
     CDS             24..236
                     /locus_tag="OO448_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            328..3012
                     /locus_tag="OO448_003533"
     CDS             328..3012
                     /locus_tag="OO448_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6338653.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3009..4097
                     /locus_tag="OO448_003534"
     CDS             3009..4097
                     /locus_tag="OO448_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6338654.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4180..5477)
                     /gene="exuT"
                     /locus_tag="OO448_003535"
                     /pseudo
     CDS             complement(4180..5477)
                     /gene="exuT"
                     /locus_tag="OO448_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5960..7372
                     /gene="uxaC"
                     /locus_tag="OO448_003536"
     CDS             5960..7372
                     /gene="uxaC"
                     /locus_tag="OO448_003536"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6338655.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7387..8874
                     /gene="uxaA"
                     /locus_tag="OO448_003537"
     CDS             7387..8874
                     /gene="uxaA"
                     /locus_tag="OO448_003537"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6338656.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8957..9508
                     /locus_tag="OO448_003538"
     CDS             8957..9508
                     /locus_tag="OO448_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6338657.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9513..10757)
                     /gene="sstT"
                     /locus_tag="OO448_003539"
     CDS             complement(9513..10757)
                     /gene="sstT"
                     /locus_tag="OO448_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6338658.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2916 a         2669 c         2856 g         2575 t
ORIGIN      
        1 agcggtatta agcgcagcta tttagcaaga ttgttccgct gacagtaacc tggggcggtg
       61 aagaaattaa agctgatgcg gcaaccactt ttaccgcaac caaaatcttt gcttccgatg
      121 cgttaactaa cggttctctg gctaaaaacc tgatgtttgc tcaaaccacc aaaggtgttc
      181 tggagacagg catctaccgt ggtgtagtaa gcatttatct gtctcaggat atctaatcca
      241 gataacagaa ttaacacgta acaaagggag gggtagccct cccttatttt aactgtttta
      301 gtatttgtta tggatttcag gattgccatg gataaaaaat tactggctct tttgatcctg
      361 gcgagtctca gcccggcaga ggcgacatta accgaaattc ccgcagggtt tgaggttatt
      421 gctcagggac agcaggagta tatcgaggtt tatttttcag ggaaaagtct cggtaaatat
      481 tatgcaatgg ttaatcttga taccgtaaca tttcttgatc cagcaagtct atataacaag
      541 ctggaactgg atgtagacga tcagaaaatc gcgcatatag tgaaagaaaa attatcgcag
      601 ccgctagctc accacggtga attggcttgc ggttatgtac gtactgactc agggtgtggt
      661 tttctgaata ccgatacgct ggaaataatc tataatgatg aagaaagttc ggcaacgttg
      721 tttattaatc cgcaatggaa ttcagctttc gatgcgaagt cattatattt aaatccagac
      781 aaaaatactg ttaatgcttt tatacatcag caagacatca atgttctggc acaggatgat
      841 taccaatcgt tgtctattca gggaaacggt gcgctgggaa taacagaaaa tagttatatt
      901 ggtgcacact ggaatttcaa cggttatgat gcagatgatg tcagtgacag taatgctgac
      961 gtcagcgatc tctattatcg ttatgatttt ttacgtcgtt attatgtgca ggcgggtcgc
     1021 atggacaacc gcacactatt taatgcacaa ggcgggaact ttacctttaa ctttctgcca
     1081 ctcggtgcaa ttgatgggat gcgtatcggg tcgacactga gctatttaaa ccaggcgcaa
     1141 agccagcaag gaaccccagt aatggttctg ctttcgcgca attctcgtgt tgacgcttat
     1201 cgtaatgagc aacttttggg atcgttttat ctcaatagtg gttcgcaatt tattgatacc
     1261 agttcctttc cgccaggcag ctacagcgtg gcattaaaag tctacgaaaa taaccaactc
     1321 acccgcaccg agcttgtgcc gtttaccaaa accggcggtc tgactgacgg aaatgcgcaa
     1381 tggttcttac aggcaggtaa aactacatca caggtttctg atgatgaaag ttcagcttat
     1441 cagctaggag tacgcctgcc attacatccg caatatgagc tctacgcagg gctggcgaat
     1501 gccgatgatg tgagtgcttt cgagttaggt aataactgga cggcagattt aggcggggtg
     1561 gggaatcttg caatcagcgc cagcgtgttc cgtaacgatg acggcggcaa aggtgatatg
     1621 caacaggcca actggagtaa tccgggatgg ccgacgttgg gcttttatcg gaccaactct
     1681 gacggcgatg cttgtacaac cgacagcaga gagagctata acgccttaag ctgttatgaa
     1741 agtatttccg cgacggtttc acagaatttt gtcggctgga atatgatgct gggttatacc
     1801 cgcacacaaa ataacactga tgatagtttg cgttgggata aacagcagag ctttgaaaat
     1861 aactatcttc gccagacaac agcgcaaagt atctctgaaa ctgtacaact tagcgcttcc
     1921 cgcgcttttg tgatgcgtga ctggattttg agtacgtcag tcggtgtttt ccatcgtaat
     1981 gacaacggtg gcgataacga cgacaacggc ttgtacttat cgttttcgtt atctgacacg
     2041 ccaacgatgg atagcaataa caacagccat tcaacaaatg tgtctacgga ttatcgttat
     2101 agcgaacagg atggcgatca aacgtcatgg cagttatcgc atacctttta taacgattca
     2161 ttcagccata aagaacttgg cgtaaccgtc ggaggcctga acaccgatac cataaacagc
     2221 gcggttaacg ggcgttggga tgggcaatac ggaaatgtct acgctaccgt atctgatagt
     2281 tatgaccgta agaatcatga tcatctttcg gcctttacgg gcacttatag ctctacgctg
     2341 gctgtcagtt gctatggcgt taatttggga gccagtggta cagacgattt gctgggtgcg
     2401 gtactggtgg atgtgaaagg cttctctgaa caggatgaag agagtcagga tctgcaactc
     2461 gaagcgcggg tggcaggcag ccgaactttg cagcttggtc aaagtgacag tgtgttgttc
     2521 ccttatcctg gatttcagtc tggttttgtt gaggttaacg acagtagcca gggcaatcag
     2581 caggggacaa caaacatcat taacggtgca gggaatcgtg aattaatgtt gttgcctggc
     2641 aagctgcgct atcgcgaagt gtctgccagc tttaactaca actatatcgg tcgtttatta
     2701 ctgccggcag cggtgaaaaa attcccgatt gtggggctga acagcgccat gttactggta
     2761 gctgaagatg gaggatttac acttgaaatt aacggcagcg aaaaagagct ctatctgctt
     2821 tccgggcagc aattccttaa gtgtccactg agtgttgtaa agaaacgcgc cagcattcgt
     2881 tacagcggag atgttacttg tagtgtggtg acttattcac aattaccgga gtcgattcag
     2941 gttcaggcac agttgaaaca gcctaaatta cgtggaaacg ttcagacggc gcaaagggag
     3001 gttgcaccat gagaaatcga ttgattgcgg cgatattggg cttgtttggc acgctcactg
     3061 gcgttcaggc agctcctgac gtgaccagtg aaattacgta tgatttggca tctggtagag
     3121 cggattatta cttctggaag gatgaagcgt cggcaggaaa taatggatat atgtggtatg
     3181 aatgttcgta tcctgacctc caacaaacct gtacagctaa tggaaatata tcgacagtac
     3241 aaatctattt aactgaacaa cgcagtggga tgcgttggcc ggtaaaactc aaaggattta
     3301 aaacagccat tgtaagtagc gatgaagcgc cgccaggatg caaggggggc aaagggcttc
     3361 agacgaatct taaggattct aatagatctt catgtacaga agatggtcaa cattattata
     3421 tatacgatac aaagtttctt acgctctacc ttgagcagac agagatgaag aatttgccga
     3481 ttggtggcgt ctggaagggg aaagttaaat tacattcgaa cagcccggcc caggactatt
     3541 tcgcaaatat taccctgaat acgctcgacc ccaaccatat tgacgtgttc ttcccggagt
     3601 tcgcccacgc cacgccaagg gtgcagttag acttgcatcc aacaggaagc gttaacggca
     3661 gcaactacgc gcaagatctg accatgttgg acatgtgcct gtacgatggt tttaacggta
     3721 atgccatcag ttatgaaatc atgctcaaag atgaagggcg accagctgca gggcgcagag
     3781 acggtgactt ctctatctat cgtcaaggag ggaccaccac cgacgaggga gaacgcattg
     3841 attaccgggt caaaatgtac aacccggaaa ccggtgggca aattgatgtg cgcaataatg
     3901 aaaatatggt ctggaacagc attaacctga aacgtgtgcg tccggtggta ctgcccggta
     3961 ttcgctatgc cgtgatgtgt gtgccgacac ccttgactct ggcagtcgat aaattcagcg
     4021 tgatggataa acaggccggg tactacatgg gcaaattgtc agtaatcttt acgccttcct
     4081 tgccaaccat caattgaccc cgcagaccag aaaaaactaa acaacgaagc cgcctgataa
     4141 ggcggcttcg tcattacaac ttacgttaaa cgttaaacgt taatgttgcg gtgcgggatc
     4201 gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc tgcaatacgg tccagataac
     4261 cagcgcaccc agcaggtcaa atactgccag aactgcgaac agcgggctga agccgatggt
     4321 gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt gccagccatg cggacatccc
     4381 ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac acgtcagaag agagcgtaat
     4441 cagcgcgcca gacagtgcct ggtgggcaaa accaccgata cacagcagca taattgcgac
     4501 atacgggttg gtgaacagac cgatcatacc cgggccaatc atcagcacag cacccagcgt
     4561 tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg
     4621 caggtaacca ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcgaacat
     4681 cgcgatctct ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt
     4741 aaaagtaccc caggccggtt ctgccaggaa acgcggcagt gcgatacccc agaactgacg
     4801 gttacgcagg atctgaccaa cggacatttt cttcgccgtg ctcacctggt gctgggcttc
     4861 ctgaccatta ataatatagt cgcgttcttc atcggtcaga tgcttctggt cgcgcggatg
     4921 tttatagaaa atcagccatg ccatcgccca gataaagctc aatgcaccgg agatgataaa
     4981 tgccatctgc cagctgtgca tcacgattgc cataccacca gcggcggcgc aatcatcgca
     5041 ccaatcgaag aacctacgtt aaagtagcct actgcgatgg aacgctcttt cgccgggaac
     5101 cattcggagc tggctttcag acccgccggg atcatcgctg cttccgcggc accgaccgca
     5161 ccacgagcaa cagccaggcc accccagcta cctgccagcg cggttgcacc acagaacacg
     5221 gcccacagca cagcaaacat tgcataaccg attttcgtac ccagcacatc cagtacataa
     5281 cctgctaccg gttgcatgac cgtataagca gcagaatagg ctgcgatgat ataggaatac
     5341 tgttgggtgg agatgtttaa ctcttccatc agagttggcg cagctgccgc cacagtgtta
     5401 cgcgtcaggt aaccaagcac ggtgccgagc gtcaccagtg cgatcatata ccaacgtaac
     5461 cctttaattt tacgcatgta aaacctcatc gttttgttat atccgcagga agcgcggcca
     5521 tcatcccgcc agaggctgaa gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac
     5581 gccccgaacg ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat
     5641 tcatctaaca aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc
     5701 agaaagataa cttgtcatac aactttaaaa ggtgagagcc atcacaaatg tgcgaatatt
     5761 tgtagggaca ttacctgacg acagcgaggc cagtactggc gcggcctgca gcgagattta
     5821 tcactttgag agtaattttt ttaactacgt ttattgatct aactcacgaa aatatcttcg
     5881 gactctggaa attggtgtga taactttgtc agcatcgcac cataagcaag ctagctcact
     5941 cgttgagagg aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga
     6001 atttgcccgc cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg
     6061 ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg
     6121 gctgaaaggc gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ccgagcgtct
     6181 gtgtaccggt gatgcatctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca
     6241 cactatcggc aacccgttat accactggac gcaccttgaa ctgcgtcgtc cgtttggtat
     6301 cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact
     6361 gctggcgcag gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt
     6421 cggcaccacc gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg
     6481 ctctttcacc atcaaagtgc tgccgagctg gcgtccggac aaagccttca acatcgaaca
     6541 ggcgaccttt aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg
     6601 ctttgctgac ctgcaaactg ccttgaccaa acgtctggat cacttcgccg ctcacggctg
     6661 taaagtgtct gaccatgcgc tggacgtggt gatgtttgct gaagcgaacg aagcggaact
     6721 ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg agcgagcacg aagtggcaca
     6781 gttcaaaact gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca
     6841 gcagtaccac attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc
     6901 ggatgtaggc tttgactcca tcaacgaccg tccgatggcg gaagagttgt ctaagctgct
     6961 gagcaagcag aacgaagaaa acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg
     7021 cgataacgaa gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa
     7081 aatgcagttc ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat
     7141 gacccaactg gcgcagctcg gtctgctgag ccgctttgtc ggtatgctga ctgacagccg
     7201 tagcttcctg tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg
     7261 tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa
     7321 aaatatttgc tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg
     7381 gttgatatgc aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat
     7441 ttggctgaag gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt
     7501 gctcgtggac ataaatttgc gttaacggat atcgcaaaag gggccaacgt catcaaatat
     7561 ggcctgccga ttggttatgc attggcggat attgcggcgg gggaacacgt ccacgcccac
     7621 aatacgcgca cgaatctgag cgatctggat cagtatcgct atcaacctga ttttcaggat
     7681 ctgcctgcgc aagcggcaga tcgtgaagtg cagatctatc gtcgcgctaa cggcgatgtc
     7741 ggggtgcgta acgagctgtg gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg
     7801 caaatccaga accgtttcct gaaagagacc aacaacgccg aaggtaccga cggcgtgttc
     7861 ctcttcagcc acacctacgg ctgctcacaa ctgggcgacg atcacatcaa catccgcacc
     7921 atgctgcaaa acatggtgcg ccacccgaac gcgggcgcag tgctggtgat tggtctgggc
     7981 tgtgaaaaca accaggttgc cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc
     8041 gttcatttca tgatctgcca acagcaggat gatgagatcg aagccgggat cgagcatttg
     8101 catcagctgt ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg
     8161 aagtttggtc tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg
     8221 ctggggcgtt tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg
     8281 ccggagatgt ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt
     8341 gaaaaactgg tcaccatggt caacgacttt aaacagtact ttattgccca tgatcagccg
     8401 atctacgaaa acccatcacc ggggaacaaa gcgggcggta tcaccacgct ggaagacaaa
     8461 tcacttggct gtacccagaa agcgggttcc agtgtcgtgg ttgacgtgct gcgttacggc
     8521 gagcgtctga aaacgcctgg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg
     8581 accagcgccc tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgcggcacg
     8641 ccatacggtg gatttgtgcc gacggtgaaa atcgccacca acagtgaact ggcggcgaag
     8701 aaaaaacact ggatcgactt tgacgcgggt cagctgatcc acggtaaagc gatgccgcag
     8761 ttgctggaag aatttatcga caccatcgtt gagtttgcca acggtaagca aacttgcaac
     8821 gagcgtaacg acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga
     8881 aatgagttgt taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac
     8941 ctgataagga tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt
     9001 tctgattggt atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc
     9061 ggtctatagc gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc
     9121 cagtgccatc cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt
     9181 aatggcgatt tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt
     9241 cgaactattg ccggttatcg gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg
     9301 gctcaccatg cgctgcgtga tgtggttttc aacgtgttgc tgggtgattc acaacttctg
     9361 ggcggggtcg ataggcggca caatgattga gggtagtttt ctgcttatga atggcttgaa
     9421 tatcattcgt ttctggcgga tgcagaaaag gggaattgat ccgtttaaag tagagaaaac
     9481 ccctcccgcc gtagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga
     9541 cgatcgtctt ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc
     9601 gcggtttcgc aagagtcctg caatacgccg atgataaagc cgacggcaac cacctgcatg
     9661 gcgatatcgt tcgaaatacc gaacatatta caggccagcg ggatcagcag cagagaaccc
     9721 cccgccacgc cggatgcgcc acaggcacac agagaagcca ccacgctcaa cagcagcgcc
     9781 gtgggcagat cgaccgggat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg
     9841 gtgattgctg cgcccgccat attgatggtg gcacccagcg gaatagaaac ggaataggta
     9901 tcgcgatcca gattcagctt ttcacacagt gccatattca ccggaatgtt cgccgcagag
     9961 ctgcgggtga agaaggcata cacgccgctt tcgcgcaggc acagcagcac cagcgggaac
    10021 gggttacgac gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac
    10081 atacagccaa ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg
    10141 gttgccaggg tagaagaaac cagaccaaaa ataccaatcg gtgcgaagcg aatgaccagt
    10201 ttcaccataa aggtgacggc attcgacata tcgttgacca ggtttttggt ggtctcgtta
    10261 ccgtgacgca gtgcgaaacc gaggccaatt gcccacacca ggatcccgat gtagttacct
    10321 ttcagcagcg cgtcgatggg gttggaaacc atgctcatta ccagcccgcg catcacttct
    10381 acaatgcctg acggtggcga aatatcaccc gcgctactgg ataagtgcag ggtagaaggg
    10441 aaggcgaagc tgaaaacgac tgcggccaga gcagcagaga aggtacccag cagatagagg
    10501 aacaaaatag ggcggatatt ggttttctgc ccatgctggt ggttagcaat agatgccatc
    10561 accagcatca acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct
    10621 aacagaccaa cagcttccgc cgcgggtttt gatatccatg ccagaagaat ccccagaaca
    10681 aggccgacca ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt
    10741 gaacgttgcg tagtcatttt tcgatccttt cattgtgtta tcgcacatcc ctggtgcgtt
    10801 ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt
    10861 acagcttcgg gaagcgaaaa atgctggatt ttaaagagaa gtcatatttt gaaacttaat
    10921 ggttacattt gtttgacata aacagaggga aggactggct actaaggctg attagataac
    10981 atatgggcaa aactatttgg aatatgagaa cggttg
//
LOCUS       DAJTVJ010000138        10914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000138 DAJTVJ010000000
VERSION     DAJTVJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.138"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..267)
                     /locus_tag="OO448_003540"
     CDS             complement(<1..267)
                     /locus_tag="OO448_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6338659.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSG"
     gene            complement(434..1174)
                     /locus_tag="OO448_003541"
     CDS             complement(434..1174)
                     /locus_tag="OO448_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6338660.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1267..1902)
                     /gene="argO"
                     /locus_tag="OO448_003542"
     CDS             complement(1267..1902)
                     /gene="argO"
                     /locus_tag="OO448_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6338661.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2041..2901)
                     /gene="mscS"
                     /locus_tag="OO448_003543"
     CDS             complement(2041..2901)
                     /gene="mscS"
                     /locus_tag="OO448_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6338662.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3259..4338)
                     /gene="fbaA"
                     /locus_tag="OO448_003544"
     CDS             complement(3259..4338)
                     /gene="fbaA"
                     /locus_tag="OO448_003544"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6338663.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4553..5716)
                     /gene="pgk"
                     /locus_tag="OO448_003545"
     CDS             complement(4553..5716)
                     /gene="pgk"
                     /locus_tag="OO448_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6338664.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5766..6785)
                     /gene="epd"
                     /locus_tag="OO448_003546"
     CDS             complement(5766..6785)
                     /gene="epd"
                     /locus_tag="OO448_003546"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6338665.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7157..7588
                     /locus_tag="OO448_003547"
     CDS             7157..7588
                     /locus_tag="OO448_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6338666.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7611..8189
                     /locus_tag="OO448_003548"
     CDS             7611..8189
                     /locus_tag="OO448_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6338667.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8190..8897
                     /locus_tag="OO448_003549"
     CDS             8190..8897
                     /locus_tag="OO448_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6338668.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8885..9562
                     /locus_tag="OO448_003550"
     CDS             8885..9562
                     /locus_tag="OO448_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6338669.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9556..10233
                     /locus_tag="OO448_003551"
     CDS             9556..10233
                     /locus_tag="OO448_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6338670.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10205..>10914)
                     /locus_tag="OO448_003552"
     CDS             complement(10205..>10914)
                     /locus_tag="OO448_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6338671.1"
                     /translation="IELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRTVV
                     FLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGAPE
                     TFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEKWC
                     QLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEESLP
                     ANLTLMMTATGEYRLVD"
BASE COUNT         2609 a         2894 c         2719 g         2692 t
ORIGIN      
        1 ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg ggaacagttt
       61 ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc gggtaaatag
      121 cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg aagccgtcat
      181 atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa tgaaattgcg
      241 catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc gcttaaccgt
      301 ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg tcatggtttt
      361 acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg acgaatacgt
      421 cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc acaggttcta
      481 actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg taagtttcct
      541 gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg ggctgatagt
      601 tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag ccgttcgcca
      661 gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg tctttatagg
      721 catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc gccttcagcg
      781 cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc accgttctca
      841 cagcgcggta gcctttcagg atacttttac catcctgata atcataatct ggctgggtgc
      901 gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg aaggaaatgt
      961 attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc gcggccacgt
     1021 taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc gtaccggagg
     1081 tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg ctaataccca
     1141 ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt taattgactt
     1201 gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt ttccatcaga
     1261 tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca gctgcaaggc
     1321 tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt ttgccgtgcg
     1381 caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca ggaaagaggc
     1441 gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc cgccaaggct
     1501 gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca ctgccagcat
     1561 ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta actcaatatt
     1621 actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg ctacgccgcc
     1681 ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc caccaaaaat
     1741 cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca taatgtggta
     1801 ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg gcagaatcat
     1861 agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata ttccgaatta
     1921 agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga taatattaat
     1981 tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc tcagcgttga
     2041 ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg ggtacgggaa
     2101 gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc agtacacgtt
     2161 ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg atgcaccaag
     2221 ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag actggataat
     2281 attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc caataataaa
     2341 ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa taattttacc
     2401 gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga aaatctgcac
     2461 actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac ggaacgggcg
     2521 gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca aagccagacc
     2581 aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta cacccacgcg
     2641 tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg cagaaagaaa
     2701 atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg cgttggaaat
     2761 catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga tgtttactgc
     2821 ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt ttatgctatc
     2881 gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat ggagaaatac
     2941 actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat aattttgcca
     3001 aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaat ccccgcagag
     3061 cggggctcag acagatatca atgtgcgctt tgtcatgccg gatgcggcgt gaacgcctta
     3121 tccggcctac aaaaacctgc aaattcaata tattgcagga tacgtgtagg cctgataagc
     3181 gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg ggccttgaga
     3241 taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga atgctttttc
     3301 cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat cgtagtattt
     3361 cttgttcggc tgatcttcgc ctttcgggtt acccaactgc ccctgcagat aagcttcgtt
     3421 cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg tatcgatgtt
     3481 catttttact acgccgtagc ttacggagtc tttgatttcc tgagcagtag aaccggaacc
     3541 accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttgg aaacatattc
     3601 ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt aaacaccgtg
     3661 tacgttaccg aaggacgcag cgatggtgaa acgcgggctg attttgctca gttcggtgta
     3721 tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt ggctgttgtc
     3781 cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca tgccgatttt
     3841 ggacatgcgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag attcttcaga
     3901 caggtcgatc atgtgagaag agaacagcgg cttaccggta gctgcgaagt gtttttcacc
     3961 cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt cagtgtgcag
     4021 gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac cagagatcgc
     4081 gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag cgataaagga
     4141 agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag cggtttccag
     4201 tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga agttgttttc
     4261 ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag gttttacgaa
     4321 atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact catcatactt
     4381 caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat gtaagctcct
     4441 ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat gaaaaaatgc
     4501 gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt ttttacttct
     4561 tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg aattcgagga
     4621 atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac aggtcgattg
     4681 ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg atagcgttag
     4741 ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc ggaccgttcc
     4801 acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa gcatcaccga
     4861 tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta gccgatgcag
     4921 tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg caggtggtca
     4981 gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc acatcgtggc
     5041 cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg tcagcgattt
     5101 tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca acgatagcca
     5161 ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca gctgccaaca
     5221 gcgggcctgc gcacgcaaca tcagcgaatt taccgatacc gtgagtagaa gcctgcgcgc
     5281 ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg tattttttgg
     5341 acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc agaacaacca
     5401 gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga accgggttag
     5461 acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg ttgtactcgc
     5521 cttcggtagg acgacccagg tgggaagtta ccatcacttt cgcgccttgt ttcagggcca
     5581 gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc ccgtctttta
     5641 ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc agatcggtca
     5701 tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca gacgctgctt
     5761 gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat tcggttagca
     5821 aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc actgacccgg
     5881 gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac agagaccaac
     5941 ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt ttgcagcaac
     6001 aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc gattgccgtc
     6061 acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa ttgcggaaaa
     6121 aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg gctggctgcc
     6181 cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg catggcggag
     6241 tgaattgtgg tcacagtgcc ggactcaata ccgtacgcat catctaacaa tttgatgacg
     6301 ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc cgcacgaagt
     6361 tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc aggatgtgaa
     6421 aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg ggagccatat
     6481 acgccggtgc agtcgaggac tacatcaacg ccaagttcac gccaggggag cgattgcagt
     6541 gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc gcgttcctgt
     6601 cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg cgccatgccc
     6661 gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg tccggattca
     6721 tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc tacgcgtacg
     6781 gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt aatccagcaa
     6841 atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt gtcgaacatt
     6901 taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg aatgattgtc
     6961 cagtcaaaga aaacaattat ctgacttacg tcacgttttc agccccgccc ctgtggaatt
     7021 tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt gccgtcacaa
     7081 accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca atacgtccga
     7141 gtttcggagt actccaatga tgacattgcc ctacccatat tcttcccccg cacgctttta
     7201 tgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt ttgcacagca
     7261 gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg acgtcgcctt
     7321 gcgctatgcc gggcaagaag gcaccacgct ccttaacggt acattcgaag tgatctcact
     7381 aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg atccacacgg
     7441 cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc ttgaactggt
     7501 gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt ctggttacga
     7561 cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt atggcgcgta
     7621 gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc aatatgatcg
     7681 gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc ggcacactta
     7741 ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg accaatttac
     7801 tctggggatt gctgaccgac ccgatcgccg ccgcatttgc acccgtcgcg atggtcatcg
     7861 gcctggtcgc cggttggctg gcgcgagcgg gttggtttcg caccctgcca aaagtcgttg
     7921 tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg cgtactgcgc
     7981 tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg cactcgatgg
     8041 ggcaaaacct ggtggagtcg gtggcgatta cagtgatagg cgcgaacctg gtcgataaaa
     8101 ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc accacgcgcc
     8161 attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta acactgtggg
     8221 cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgccg atctatagcg
     8281 cggcgacctt tttttgtctt attgcgctaa aggccacgcg gcggcgggca aaatatgtcg
     8341 cctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc tggctaacag
     8401 aatggctaag cggcacgccg cgttcgccgg agcgttggac gcatgctatt acgctctggt
     8461 tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc cccgtgcaat
     8521 gttttatccg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat ctgtttgccg
     8581 gaccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat gaagcgcagc
     8641 gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg atgcccgcgt
     8701 taattattct gctaacacac aatgcgctta acgatctggc tgtccgtggt gcggcgctgg
     8761 atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca gccgatagca
     8821 ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctaacagaa ttcggagcgt
     8881 ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc atcctccgtt
     8941 ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg acaacggcag
     9001 tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc tgccaggaga
     9061 gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc ttaatgaaca
     9121 atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg gctgtacctt
     9181 tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg aaacagagat
     9241 tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc gccatcgtca
     9301 cccggcaaca ctctccggtg gagagacaca acgcgtggtc atcgcctgcg ccatcgcgat
     9361 gcagccaaag ctcttaattc ttgatgaagc ctttagtcgc ctgacgccgc aggccagcga
     9421 aatgctgttg cagcgcttac aacactgggc gcttgaacgt ggttcgctga ttattctttt
     9481 tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat tgcaaaacgg
     9541 agcgctccag ccactatgtt aacgttaaat cagatatctt atcgctggcc tagcgcagca
     9601 acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt ggcgctgacc
     9661 ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct tcttacgccc
     9721 acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa ccgccagcgc
     9781 gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca cagcaccgtc
     9841 gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga aatcactcaa
     9901 cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc gcacccgttg
     9961 gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc actctctccg
    10021 ccactattac tgctggacga gccgagtcgg gattttgacg aaaattggtt gagcgtgttt
    10081 gaaagctggc tggagaagtg ccgccagcga ggcacgagcg tggtagcaat cagtcatgac
    10141 gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg tgtaatcagg
    10201 aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc aaatttgcag
    10261 gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca taaaaggctt
    10321 ctgcatctgc cagcgatacc cctcccgcca gcttgcgccc caccagacgc tcccgcaacg
    10381 cagtagctgg cgcgttgata aagatcgaga aatcacaaaa ttgcgcaagc tggcaccact
    10441 tctcgtcgtc cagcaacaac cagtttcctt cgacgatgac gagtggtgcg gtaacgtgca
    10501 acgcatcttc aacaggatca tgcttttgtc gatcgtactg cggccacgta caatcccctt
    10561 ccaccacctg gcgcagattt tccgccagtt tcgcaacgtc aaatgtctct ggtgcgcctt
    10621 tgaaggggcg caattgatgc gcgtccagcc agctattgta atggtgaaaa ccatccatcg
    10681 ggagcgtctg aatagcgggc agctccgggt cttgttgcac gagatattcc cagaaggtgg
    10741 ttagggtaga tttacccgtc cccggcggag cgcaaagaaa aaccaccgta cgccgctgcg
    10801 gatttaccgt ctgtaaggcc gccagcatac gcagcaacgg tttatgcacg cgttctatct
    10861 cttcatcatg gtactgcgct tgcacgttca gcccattcac cgttaattca attt
//
LOCUS       DAJTVJ010000139        10822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000139 DAJTVJ010000000
VERSION     DAJTVJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.139"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..611
                     /locus_tag="OO448_003553"
                     /pseudo
     CDS             1..611
                     /locus_tag="OO448_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="OO448_003554"
     CDS             608..1222
                     /locus_tag="OO448_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6338672.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO448_003555"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO448_003555"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6338673.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="OO448_003556"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="OO448_003556"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6338674.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="OO448_003557"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="OO448_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6338675.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="OO448_003558"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="OO448_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6338676.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6338)
                     /gene="phoE"
                     /locus_tag="OO448_003559"
     CDS             complement(5289..6338)
                     /gene="phoE"
                     /locus_tag="OO448_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6338677.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            6626..7729
                     /gene="proB"
                     /locus_tag="OO448_003560"
     CDS             6626..7729
                     /gene="proB"
                     /locus_tag="OO448_003560"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6338678.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7741..8994
                     /gene="proA"
                     /locus_tag="OO448_003561"
     CDS             7741..8994
                     /gene="proA"
                     /locus_tag="OO448_003561"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6338679.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9109..9184
                     /locus_tag="OO448_003562"
     tRNA            9109..9184
                     /locus_tag="OO448_003562"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9142..9144,aa:Thr,seq:cgt)
     gene            9790..9948
                     /locus_tag="OO448_003563"
     CDS             9790..9948
                     /locus_tag="OO448_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338680.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2736 a         2560 c         2762 g         2764 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcataacttt gccatagaca
     6241 tccagtttat taccgtcttt attatatatt tctgcagcct gtacagatgc agatgccaca
     6301 atgcccatca ccactaatgc cagagtgctc tttttcattt tcattcctga ttttaattaa
     6361 cgcgcgaata ttcagcggga gagccccgtt gaaaacagga aagtttttaa cctgagattg
     6421 ttaaagatat attacagatt aataatattc ttaaaatgtg gtaatttatt aaatctgtaa
     6481 taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac gccatgcttt
     6541 gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg atatcatttt
     6601 tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg taaaactcgg
     6661 caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatattg ttgaacttgt
     6721 tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga cgtcgggcgc
     6781 gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacta tcgcctcgaa
     6841 acaactgctg gcagcggtag ggcagagtcg actgattcaa ctgtgggaac agctgttttc
     6901 gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg aagaccgtga
     6961 acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata tcgttccggt
     7021 aatcaatgag aacgatgctg tcgctacggc agagattaaa gtcggcgata acgataacct
     7081 ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga ccgatcaaaa
     7141 aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta aagatgttta
     7201 cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc tcggaactgg
     7261 cggcatgagt accaaattgc aggccgctga cgtggcttgc cgtgcgggta tcgacaccat
     7321 tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca tttccgtcgg
     7381 tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga ttttcggtgc
     7441 gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc tggaacgcgg
     7501 cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc gtggtgaagt
     7561 catccgcatt tgcaacctcg aaggccgcga tatcgcccac ggcgtcagtc gttacaacag
     7621 cgatgcatta cgccgtattg ccggacacca ctcgcaagaa attgatgcaa tactgggata
     7681 tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag gagctggccg
     7741 atgctggaac aaatgggcat tgctgcgaag caagcctcgt ataaattagc gcaactctcc
     7801 agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa
     7861 atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gagccaatgg ccttagcgaa
     7921 gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc cgacgatgta
     7981 cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa tcgatggcgg cgtactggac
     8041 agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt gatttatgaa
     8101 gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg
     8161 atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc ggtgattcag
     8221 gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac
     8281 cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct gatcccgcgt
     8341 ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt gatcacaggt
     8401 ggtataggcg tatgccatat ttacgttgat gaaagtgtag agatcgctga agcattaaaa
     8461 gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga aacgttgctg
     8521 gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
     8581 ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg ccctgcgaag
     8641 gtggttgctg ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc
     8701 aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg cacacaacac
     8761 tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa cgaagtggat
     8821 tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca gtttggtctg
     8881 ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat ggggctggaa
     8941 gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaataaaa
     9001 ccgggtgatg caaaagtagc catttgattc ataaggccat tgacgcatcg cccggttagt
     9061 tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc cgatatagct
     9121 cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc tattatcggc
     9181 accattaaga aaattaataa cttagcttaa cttctctaag aaattgtatc ttctgttatc
     9241 acttacaaac gcctatacgt tgttatgggc tttggtagtg taacacgtat gggcgagtag
     9301 ttcccaggct caacctcgta acaagttcct ttgaagctgg aagacgtgcc accgtctcta
     9361 aaccttctac caaaactctt agcaagatgt tccacacctg gcgcggtgtc caatgatgct
     9421 acacgtcgtg agacgttgca gaggataaac aggctaaatg atgggaagag ttttaagtgc
     9481 aatagtggat taaagcccac ctcttcaaaa cctaacttga tcgggaaaga atcaagctct
     9541 agcacggttt cgtccaccgg agtacaacgg gtgcctgata agagaagaac tgctaagact
     9601 tcatagcttt tgtgaaaaag ccctttttag tggatacgcc tacggcgctt attttttggt
     9661 ataaccatca aaggcgcttt atggctccta ttgtcttgaa aatgtatctt tacttgtatt
     9721 gtgttttatg cgtagtctcc ctgtgtagta tgtaacttgt taattttcat atgaatacag
     9781 aggcacacga tgaaaaacat tgcagcaatc aaacgtaata accgcaagat tcacgctcgt
     9841 aagttcctgt ctacgccaga aggaaaagcc tggctagagc gtaaacagag agagaacgaa
     9901 gaaagaaaac tccttagtga gttgaaatgg cttagagagg atttctgaaa atctccagaa
     9961 cgcaacagga cgcattctaa cgctgttttc attactcggt atagggatag gtgatgtgtg
    10021 tttaatgcgt ctgtgcgctt gttttgtgcc ttattttgca ttttcataat gcggaagaga
    10081 acacggaaaa ccttaaaatt aattattggg gtatttaagg ctattttcag gtgatttaag
    10141 ggtattaaac ttgatttaat tgattaaaat ataaacagat aattatgtat cttattgatt
    10201 tataaggtta tttttaagta aaggtaaata aaaaataaat agccataaat agctataaat
    10261 acatcttaat acctatatcc cttcccttat ggctagattg cgagaggtgc ttaacgcgat
    10321 tttcagcgcc ccccgatctg gaaaatccag aatccccaaa tatcagcaag gaattttctt
    10381 gcgtaatcaa catcgttttt acctattaaa tcattacaca gaaagccttt ctgtaatgtc
    10441 tctcactaaa ctttcactaa acacgagaat gatcggtttt atgtcggatc agatgctaga
    10501 aacagcacca cgtctcacaa gggctgtaag cgatgaaacc agtgtttatg cgggtgcagg
    10561 tcaaaatata ggccaaaatc catttaacat aataaatgtt atatgtgcta aggaacacat
    10621 ataacgcctt gaattgatgt atcagtggaa aagtgatgga ttttttacgt aggagcacga
    10681 aagggcttct attgactttt gtgtgctatg aaatataatt aaagtttttt gtttttattg
    10741 tgtgatgtgg tgggttacac aggggaatag attaaatggt gttctgttta tacaggttta
    10801 tttgttagat caacaatttg gc
//
LOCUS       DAJTVJ010000140        10821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000140 DAJTVJ010000000
VERSION     DAJTVJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.140"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(34..1317)
                     /gene="aroA"
                     /locus_tag="OO448_003564"
     CDS             complement(34..1317)
                     /gene="aroA"
                     /locus_tag="OO448_003564"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6338681.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1388..2476)
                     /gene="serC"
                     /locus_tag="OO448_003565"
     CDS             complement(1388..2476)
                     /gene="serC"
                     /locus_tag="OO448_003565"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6338682.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2675..3367)
                     /locus_tag="OO448_003566"
     CDS             complement(2675..3367)
                     /locus_tag="OO448_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR6338683.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3497..5257
                     /locus_tag="OO448_003567"
     CDS             3497..5257
                     /locus_tag="OO448_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6338684.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5663..6520
                     /gene="focA"
                     /locus_tag="OO448_003568"
     CDS             5663..6520
                     /gene="focA"
                     /locus_tag="OO448_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6338685.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6575..8857
                     /gene="pflB"
                     /locus_tag="OO448_003569"
     CDS             6575..8857
                     /gene="pflB"
                     /locus_tag="OO448_003569"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6338686.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9049..9789
                     /gene="pflA"
                     /locus_tag="OO448_003570"
     CDS             9049..9789
                     /gene="pflA"
                     /locus_tag="OO448_003570"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6338687.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9882..10058)
                     /locus_tag="OO448_003571"
     CDS             complement(9882..10058)
                     /locus_tag="OO448_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6338688.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10158..>10821
                     /locus_tag="OO448_003572"
     CDS             10158..>10821
                     /locus_tag="OO448_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6338689.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     ILD"
BASE COUNT         2785 a         2708 c         2563 g         2765 t
ORIGIN      
        1 gaaagatttg gctatttatt gcccgttgtt cattcaggct gcctggctaa tccgcgccaa
       61 ctgctcgaaa taatccggaa atgttttggc cgtacatttg ggatcaagaa tcgtcactgg
      121 tgtatctgac aacgccacca gcgagaaaca catcgccatc cggtgatcat tgtatgtcgc
      181 gatctcggca aagttcagtt tttccggtgg agtgatacga atgtaatcgt gcccctcttc
      241 tacttccgca ccgactttac gcagttctgt tgccatcgca aacaggcgat cggtctcttt
      301 aacacgccag ttatagatat tgcgcagcgt ggtggtgcct tttgcaaata acgccgccgt
      361 ggcaatggtc atcgccgcat cgggaatatg gttcatatcc atatcaatag cgttcagttc
      421 accacgcgtg caggaaatat aatcatcgcc ccagcaaatg gtcgcgccca ttttttccag
      481 cacatcagca aagcgaatat caccctgcat actgttacgt ccaataccgg tcacttttac
      541 agtgccgcct ttgattgctg ctgctgccag aaagtaagaa gccgaagatg catcgccttc
      601 gaccaaataa gtacccggag actgataaga ctgcccgcct tttacgacaa attgttgata
      661 gtgctgattt tcaatttcaa caccaaacgt cttcatcaga ttgagtgtga tgtcgatata
      721 aggtttagaa accagatcgc ctttaatacg aatcaccgta tcttccggcg caagaggcgc
      781 agtcattaac agtgcggtga ggaattggct ggaaacggag ccatcaacgt caacgttgcc
      841 gccagtaaag ccgccctgta aacgcaacgg cggataattt tcttgttcca ggtaagtgat
      901 cttcgccccg cccaggcgca gcgcatccac cagatgacca atcgggcgtt ctttcatacg
      961 cggctcaccg gtcagcacaa tatcattgct acccagacaa agagctgccg ccagcggacg
     1021 cattgccgtt ccggcgttac cgaggaacaa ctccagggca ccttctgcgt gtaatggacc
     1081 gccgttaccg ataatttcgc aacgcgtacg atcggctgaa agcgtatagc ttacccctaa
     1141 cgctgttaat gcattcagca tatggcgcac gtcatcgcta tccagcagat tggttaatac
     1201 tgttttgccg tgtgctaatg ccgccagcaa taaagcgcgg ttagaaacgc tcttggaacc
     1261 gggcagatta atagtgccat cgacacgagc gatgggttgt aacgtcaggg attccatgaa
     1321 ctcaactctc tacaacagaa ataaaaaccc cacaggctgg ctgtggggat taagcaaaat
     1381 ttcggcatta accgtgacgg cgttcgaact caaccatgaa gtctgtcagc gctttaacgc
     1441 cttccagcgg catggcgtta taaatagaag cgcgcattcc gccgaccaca cggtgacctt
     1501 tcagtgcatg aaggccagca gcaaaagact cttcaaggaa caatttgtca agcgcactgt
     1561 ccgccaactg gaacggcacg ttcatccgcg aacggttagc tttcgccacg tcattgcggt
     1621 agaaatcgct gttatcaatc accccatata gcagttctgc tttttgctga ttgattttat
     1681 ccatttcagc tacaccgccg ttcgctttca gccatttaaa gaccagacca gatagatacc
     1741 aggcaaatgt cggcggcgtg ttaaacatgg agccgttatc gttgaggatg gaataatcca
     1801 gaatcgacgg acacgcgata ttcgctttgc ccagcaaatc ttcacgaacg atgacgattg
     1861 tcaggccagc cgggccgata tttttctgcg cgccagcgta aatcacgcca taacggctga
     1921 cgtcaatcgg acgggaaaga atggttgaag agaagtcggc ggcgaccacc acatctttgc
     1981 cgaagtctgg cgtttcgtcg atggcgatac cgtcgatggt ttcattcggg cagtaatgca
     2041 tataagcagc attatcagag agttgccatt cacgcattgg cttaaccgcg cgcagaccat
     2101 caacagtcac tttggcgtca aagacattag gcgtgcagta ttttttcgct tctttaatgg
     2161 cacttgccgc ccagtaaccg gcatcaacat aatctgcggt ggttttatca ccgagaatat
     2221 tcagcggtac cgcagcaaac tgaccgcgac caccgccatg gcagaataat accttgtagt
     2281 tggaggggac attaagaaga tcgcgaaaat ccttctcggc ttcctctgca acctgaatga
     2341 actctttgcc acggtgactc acttccatca ccgacgtacc aagaccgttc cagtcgcgca
     2401 gttcctgttg agcctgttta agcacctctg ccggtagcat tgccggacca gaactaaaat
     2461 tgaagatttg agccattgcc cctcaccacg ttgcgttgct attaacccgc ggtcaatcga
     2521 ttgcgaccac aggtaataca ttattcctgt ggctatcggt tttatcattc agtgacacac
     2581 atcgcaatga gtaaaaccgt tgagggcaaa aatgtggctt gcatcacaca aaagaatctc
     2641 tcggattatt agcaaaaagc cgatgttttt actgtcaggt cacccgtttt gcccgacttg
     2701 ccttcgtcca ttctggattt gcacagcgcg gacataattg tttttccccc gctttcatat
     2761 gaatgatttc actacaacga atacaggcat agtccgccga ctccggcacc actttgtaac
     2821 gctgcgtaca atcactgggt aaaataagta aacccggttt caccttgtca tcttcaaaga
     2881 aatagacact gatttgcccg ttggtttcga gaatcgccag acgtacctgc cccagctgct
     2941 ccacgccacg caatcgtagc tccataaaga actcaaattc cgtcatgttg gagttattga
     3001 gtttcgacca ggccagctcg ccatcttcaa taataacaac cggcttacct tccagtagat
     3061 cttccagttt ttcactgtgc gccatcaacc acattaccag gcggtataac aacgccagag
     3121 tgataaagac gataagtacc ggcaccatcg gcacatcatc ataaaacgcc acatctcccg
     3181 ccgctgaacc cagcgtgaga atgattaaca cttcaaacag cgacatctgt cgcacaccgc
     3241 gtcttccggt cattttgagg aacaaaaaaa ccagtacaaa ggtataaaga ctacgtagtg
     3301 caacttcgcc aaggaaatca aaaggcactt tatcaaatgc catacggtgg agatcgaacg
     3361 ccttcatttt tatgctctta tcgtggttta tccgctaaaa gcatagtcaa cagttcatcg
     3421 gcatggcggc ggtgatagcg cctttgagat aaaacccgta ttattgcgcg ctttccgtac
     3481 gactaaagtg attttcatga cgcaaacatt tatccccggc aaagatgccg ctctggaaga
     3541 ttccatcgct cgcttccagc aaaaactttc agacctcggc tttcagattg aagaggcctc
     3601 ctggctgaat cctgtgccta acgtctggtc tgtacatatt cgcgacaaag agtgcgcact
     3661 gtgttttacc aacggtaaag gcgcaaccaa aaaagcggcg ctggcttctg cacttggtga
     3721 atatttcgag cgtctctcaa ccaactactt ttttgctgat ttctggctgg gcgaaaccat
     3781 cgccaacggt ccgttcgtgc attatcccaa cgaaaaatgg ttcccactga ccgaaaatga
     3841 cgatgtgcca gaaggactac tcgatgaccg tctgcgcgcg ttttatgatc cggagaatga
     3901 actgaccggc agcatgctga ttgacctaca atccggtaac gaagatcgtg gtatttgcgg
     3961 cctgccgttt acgcgtcagt ccgacaatca gaccgtttat attccgatga atatcattgg
     4021 taacctgtac gtctccaacg gtatgtccgc aggtaatacc cgcaacgaag cacgcgttca
     4081 gggattgtct gaagttttcg aacgctacgt gaaaaaccgc attattgctg aaagcatcag
     4141 cctgccagag atcccggcag acgtgctggc gcgttaccca gcagtagttg aagccatcga
     4201 aacgctggaa gcggagggtt tcccaatctt cgcttatgac ggttcgcttg gcggccagta
     4261 tccggtgatt tgcgtggtac tgttcaatcc tgctaacggt acctgctttg cctctttcgg
     4321 tgcgcatcct gattttggcg tagcactgga acgtaccgtg accgagctgc tgcaaggtcg
     4381 tggcctgaaa gatttggatg tgtttactcc gccaaccttc gatgatgaag aagtcgctga
     4441 acataccaac ctcgaaacgc actttatcga ttccagcggt ttaatctcct gggacctgtt
     4501 caagcaggat gccgattatc cgtttgtgga ctggaatttc tccggcacca cggaagaaga
     4561 gtttgctacg ctgatggcta tcttcaacaa agaagataaa gaagtttata ttgccgatta
     4621 cgagcatctg ggcgtttatg cttgccgtat tatcgtgcct ggcatgtccg atatttatcc
     4681 ggctgaagat ctgtggctcg cgaataacag tatgggcagc catttacgtg aaacgattct
     4741 ttcgctacca ggcagcgagt gggaaaaaga agattacctg aacctcatcg agcaactgga
     4801 tgaagaaggt tttgatgact ttacccgcgt gcgtgagctg ttgggtctgg cgaccgggtc
     4861 ggataacggt tggtacaccc tgcgtatcgg tgaattaaaa gccatgctgg cgctggctgg
     4921 tggcgatctg gaacaggctc tggtctggac cgaatggacg atggagttta actcatcagt
     4981 atttagtccg gaacgcgcca actattatcg ctgcctgcaa acgttgttat tactggcaca
     5041 ggaagaagat cgccagccgc tgcaatatct gaatgcgttt gttcgcatgt acggcgcaga
     5101 tgccgtagaa gccgccagtg cggcaatgag cggcgaagcg gcgttttacg gcctgcaacc
     5161 ggtagatagc gatctgcacg cgtttgctgc acatcagtcg ttgttgaagg cctacgaaaa
     5221 gctgcagcgc gccaaagcag cattctgggc aaaataaaat caaatagcct acgcaatgta
     5281 ggcttaatga ttagtctgag ttatattacg gggcgttttt ttaatgcccc gctttacata
     5341 tatttgcatt aataaaataa ttgtaattat aaggttaaat atcggtaatt tgtatttaat
     5401 aaatacgatc gatattgtta ctttattcgc ctgatgctcc cttttaatta actgttttag
     5461 cggaggatgc ggaaaaaatt caactcattt gttaattttt aaaatttatt tttattttga
     5521 taatcaaatg tttactccgt atctgcataa aaaccatgcg agttacgggc ctataagcca
     5581 ggcgagatat gatctatatc aatttctcat ctataatgct ttgttagtat ctcgtcgccg
     5641 acttaataaa gagagagtta gtgtgaaagc tgacaaccct tttgatcttt tacttcctgc
     5701 tgcaatggcc aaagtggccg aagaggcggg tgtctataaa gcaacgaaac atccgcttaa
     5761 gactttctat ctggcgatta ccgccggtgt tttcatctca atcgcattcg tcttctatat
     5821 cacagcaacc actggcacag gcacaatgcc cttcggcatg gcaaaactgg ttggcggcat
     5881 ttgcttctct ctggggctga ttctttgtgt tgtctgcgga gccgatctct ttacttccac
     5941 cgtgttgatt gttgttgcta aggcgagtgg gcgcatcacc tggggtcagt tggcgaaaaa
     6001 ctggctaaat gtctattttg gcaacctggt cggcgcactg ctgtttgtac ttttaatgtg
     6061 gctttccggc gagtatatga ccgcaaatgg tcaatgggga ctaaacgtcc tacaaaccgc
     6121 cgaccacaaa gtgcaccata cttttattga ggccgtctgt cttggtatcc tggcaaacct
     6181 gatggtatgt ctggcagtat ggatgagtta ttctggccgc agcctgatgg acaaagcgtt
     6241 cattatggtg ctgccggtcg cgatgtttgt tgccagcggt tttgagcaca gtatcgcaaa
     6301 catgtttatg atcccgatgg gtattgtaat ccgcgacttc gcatccccgg aattttggac
     6361 cgcagtcggt tctgcaccgg aaaatttttc tcacctgacc gtgatgaatt tcatcactga
     6421 taacctgatt ccggttacga tcggcaacat tatcggtggt ggtttgttgg ttgggttgac
     6481 atactgggtc atttacctgc gtgaaaacga ccaccattaa tggttgtcga agtacgcagt
     6541 aaataaaaaa tccacttaag aaggtaggtg ttacatgtcc gagcttaatg aaaagttagc
     6601 cacagcctgg gaaggtttta ccaaaggtga ctggcagaat gaagtaaacg tccgtgactt
     6661 cattcagaaa aactacactc cgtacgaggg tgacgagtcc ttcctggctg gcgctactga
     6721 agcgaccacc accctgtggg acaaagtaat ggaaggcgtt aaactggaaa accgcactca
     6781 cgcgccagtt gactttgaca ccgctgttgc ttccaccatc acctctcacg acgctggcta
     6841 catcaacaag cagcttgaga aaatcgttgg tctgcagact gaagctccgc tgaaacgtgc
     6901 tcttatcccg ttcggtggta tcaaaatgat cgaaggttcc tgcaaagcgt acaaccgcga
     6961 actggatccg atgatcaaaa aaatcttcac tgaataccgt aaaactcaca accagggcgt
     7021 gttcgacgtt tacactccgg acatcctgcg ttgccgtaaa tctggtgttc tgaccggtct
     7081 gccagatgca tatggccgtg gccgtatcat cggtgactac cgtcgcgttg cgctgtacgg
     7141 tatcgactac ctgatgaaag acaaactggc acagttcact tctctgcagg ctgatctgga
     7201 aaacggcgta aacttggaac agactatccg tctgcgcgaa gaaatcgctg aacagcaccg
     7261 cgctctgggt cagatgaaag aaatggctgc gaaatacggc tacgacatct ctggtccggc
     7321 taccaacgct caggaagcta tccagtggac ttacttcggc tacctggctg ctgttaagtc
     7381 tcagaacggt gctgcaatgt ccttcggtcg tacctccacc ttcctggatg tgtacatcga
     7441 acgtgacctg aaagctggca agatcaccga acaagaagcg caggaaatgg ttgaccacct
     7501 ggtcatgaaa ctgcgtatgg ttcgcttcct gcgtactccg gaatacgatg aactgttctc
     7561 tggcgacccg atctgggcaa ccgaatctat cggtggtatg ggcctcgacg gtcgtaccct
     7621 ggttaccaaa aacagcttcc gtttcctgaa caccctgtac accatgggtc cgtctccgga
     7681 accgaacatg accattctgt ggtctgaaaa actgccgctg aacttcaaga aattcgccgc
     7741 taaagtgtcc atcgacacct cttctctgca gtatgagaac gatgacctga tgcgtccgga
     7801 cttcaacaac gatgactacg ctattgcttg ctgcgtaagc ccgatgatcg ttggtaaaca
     7861 aatgcagttc ttcggtgcgc gtgcaaacct ggcgaaaacc atgctgtacg caatcaacgg
     7921 cggcgttgac gaaaaactga aaatgcaggt tggtccgaag tctgaaccga tcaaaggcga
     7981 tgtcctgaac tatgatgaag tgatggagcg catggatcac ttcatggact ggctggctaa
     8041 acagtacatc actgcactaa acatcatcca ctacatgcac gacaagtaca gctacgaagc
     8101 ctctctgatg gcgctgcacg accgtgacgt tatccgcacc atggcgtgtg gtatcgctgg
     8161 tctgtccgtt gctgctgact ccctgtctgc aatcaaatat gcgaaagtta aaccgattcg
     8221 tgacgaagac ggtctggcta tcgacttcga aatcgaaggc gaatacccgc agtttggtaa
     8281 caacgatccg cgtgtagatg acctggctgt tgacctggta gaacgtttca tgaagaaaat
     8341 tcagaaactg cacacctacc gtgacgctat cccgactcag tctgttctga ccatcacttc
     8401 taacgttgtg tatggtaaga aaacgggtaa caccccagac ggtcgtcgtg ctggcgcgcc
     8461 gttcggaccg ggtgctaacc cgatgcacgg tcgtgaccag aaaggtgcag tagcctctct
     8521 gacttccgtt gctaaactgc cgtttgctta cgctaaagat ggtatctcct acaccttctc
     8581 tatcgttccg aacgcactgg gtaaagacga cgaagttcgt aagaccaacc tggctggtct
     8641 gatggatggt tacttccacc acgaagcatc catcgaaggt ggtcagcacc tgaacgttaa
     8701 cgtgatgaac cgtgaaatgc tgctcgacgc gatggaaaac ccggaaaaat atccgcagct
     8761 gaccatccgt gtatctggct acgcagtacg tttcaactcg ctgactaaag aacagcagca
     8821 ggacgttatt actcgtacct tcactcaatc tatgtaatta gatttgactg aaatcgtaca
     8881 gtaaaaagcg tacaataaag gctccacgaa agtggggcct tttttagcac gagagccttt
     8941 tttgtcagct atctatactc taaggtgact gccaaaacag actcgacgta gccttcgagc
     9001 tgcgcaccaa cacggcctca gatgggccac atctggagaa acaccgcaat gtcagttatt
     9061 ggtcgcattc actcctttga atcctgtgga accgtagacg gcccgggtat tcgctttatc
     9121 acctttttcc agggctgcct gatgcgctgc ctgtattgtc ataaccgcga cacctgggat
     9181 acgcatggcg gtaaagaagt taccgttgaa gatttaatga aagaggtggt gacctatcgc
     9241 cactttatga acgcttccgg cggcggcgtt accgcatccg gcggtgaggc aatcctacaa
     9301 gctgagtttg ttcgtgactg gttccgcgcc tgcaaaaaag aaggcattca tacctgtctg
     9361 gacaccaacg gttttgttcg tcgttacgat ccggtgattg atgaactgct ggaagtaacc
     9421 gacctggtaa tgctcgatct caaacagatg aacgacgaga tccaccaaaa tctggttgga
     9481 gtttccaatc accgcacgct ggagttcgcc aaatatctgg cgaacaaaaa tgtgaaggtg
     9541 tggatccgct acgttgttgt cccaggctgg tctgacgatg acgattcagc gcatcgcctc
     9601 ggtgaattta cccgtgatat gggcaacgtt gagaaaatcg agcttctccc ctaccacgag
     9661 ctgggcaaac acaaatgggt ggcaatgggt gaagagtaca aactcgacgg tgttaaacca
     9721 ccgaagaaag agaccatgga acgcgtgaaa ggcattcttg agcagtacgg tcataaggta
     9781 atgttctaat ttccccgtaa agcggcaact ttattgagtt gccgcttttt tattccgcac
     9841 ttcattctgc tacagcgttt ttaacacatc ttatagtgca tctaccatat cccgtacctg
     9901 agataataac gactgataat attacgccga tataaatcga gttccgtcac ctgtattttc
     9961 cgctccattg cccgctgatg gatcgcatca gcaatatgtg cggtcaatga atcatgacga
    10021 ggatgggccc ataccaaata aatacgttca gactgcataa ttttacctcc acgttaagtt
    10081 ctggagcgca ctttacgcag gcggcataat aatgtaaacg tgatgattca ggatgagtta
    10141 atgaaggatt ttcactaatg cggatgaata tgtctgactt tgccactttc tttgctgtgg
    10201 cccgtaatca aagctttcgt gcagcgggcg atgagttagg cttatcctcg tccgccatta
    10261 gccatagtat taaaacactg gaacaacgtc ttaaaattcg cttattcaat cggacaaccc
    10321 gaagcgtatc gttaacggaa gcgggttcta atctctatga acgattgcgc ccagctttcg
    10381 acgaaattca gatcatgtta gatgagatga atgattttcg tctgacacca acaggaacgc
    10441 tcaaaatcaa tgccgcccgg gtagctgcac gtatattttt aatgccttta ctggttggtt
    10501 tcacacgtga atatcctgat attaaagtcg agctcacaac tgacgactct cttgtcgata
    10561 tcgtccaaca gggctttgac gctggcgttc gacttagtgg catcgtcgaa aaagatatga
    10621 tcagcgtggc gatcggccca ccagtgaaat tatgtgtagc agcaacgcca gagtactttg
    10681 caagatatgg caaaccccgc catcctcatg atctgcttaa tcatcaatgt gttgtatttc
    10741 gttatcccag cggcaaacct ttccactggc aatttgcaaa ggagcaggaa attgctgtcg
    10801 cagggaatat cattctggat g
//
LOCUS       DAJTVJ010000141        10689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000141 DAJTVJ010000000
VERSION     DAJTVJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.141"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..784)
                     /locus_tag="OO448_003573"
     CDS             complement(<1..784)
                     /locus_tag="OO448_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338690.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="OO448_003574"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="OO448_003574"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6338691.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="OO448_003575"
     CDS             1860..2747
                     /locus_tag="OO448_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6338692.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="OO448_003576"
     CDS             2780..3790
                     /locus_tag="OO448_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR6338693.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="OO448_003577"
     CDS             3860..5293
                     /locus_tag="OO448_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6338694.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="OO448_003578"
     CDS             5392..6741
                     /locus_tag="OO448_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6338695.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="OO448_003579"
     CDS             6731..7618
                     /locus_tag="OO448_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6338696.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="OO448_003580"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="OO448_003580"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6338697.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="OO448_003581"
     CDS             8352..9224
                     /locus_tag="OO448_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6338698.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="OO448_003582"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="OO448_003582"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6338699.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="OO448_003583"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="OO448_003583"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6338700.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2463 a         2533 c         2854 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtcat
//
LOCUS       DAJTVJ010000142        10430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000142 DAJTVJ010000000
VERSION     DAJTVJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.142"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OO448_003584"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OO448_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6338701.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OO448_003585"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OO448_003585"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6338702.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="OO448_003586"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="OO448_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR6338703.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="OO448_003587"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="OO448_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6338704.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="OO448_003588"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="OO448_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="OO448_003589"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="OO448_003589"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6338705.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="OO448_003590"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="OO448_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6338706.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO448_003591"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO448_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6338707.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="OO448_003592"
     CDS             complement(9457..10149)
                     /locus_tag="OO448_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6338708.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2549 a         2516 c         2755 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DAJTVJ010000143        10365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000143 DAJTVJ010000000
VERSION     DAJTVJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.143"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..154
                     /locus_tag="OO448_003593"
     CDS             <1..154
                     /locus_tag="OO448_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6338709.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="OO448_003594"
     CDS             258..1172
                     /locus_tag="OO448_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6338710.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="OO448_003595"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="OO448_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6338711.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="OO448_003596"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="OO448_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6338712.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="OO448_003597"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="OO448_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6338713.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="OO448_003598"
     CDS             5467..7149
                     /locus_tag="OO448_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6338714.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="OO448_003599"
     CDS             7201..8358
                     /locus_tag="OO448_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6338715.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="OO448_003600"
     CDS             complement(8324..8446)
                     /locus_tag="OO448_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338716.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8655..10340
                     /locus_tag="OO448_003601"
     CDS             8655..10340
                     /locus_tag="OO448_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR6338717.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         3028 a         2208 c         2216 g         2913 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cactgaaaat taaaaatttc tatatatttc atattgttaa
     8581 atgatactta aataaatttc atcagtaaat cattgccata gataatcatt ggatggcaca
     8641 atcaattttc gctaatgata accattccca ttacgtttta tatgttgata gcaaagtatc
     8701 tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg ttgttaatca
     8761 ttattatcct cgcaatgatc cttggcgtgg ttaaaattca ggtctggtta aacgactgga
     8821 ataatgattt tttcaatgcg ctaagccaga aagaaaccga taagctttgg cagcttgttc
     8881 tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa acatggttaa
     8941 ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt aatcgctggt
     9001 ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat acagataacc
     9061 ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc agtctttcat
     9121 ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt tggcaaagcg
     9181 cgggtacgct ctcattcact gttggtggaa cagaatggaa tatccagggg tatatggtct
     9241 ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtg ggtaaacgta
     9301 ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac atttcgaact aatcttgtgc
     9361 agcataataa gcagacagaa ctgattgcgc taagcaatgc tgagtctcta caacgccagg
     9421 agttgcgcga caattttcat accattaaag agaactggca tcgtttaatg aatcgtcagc
     9481 ggtggcttga ttactggcaa aatatttatt cgcgctcgct tagcgttctt ccctactttc
     9541 tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg aaatcgcgcc
     9601 aggcatttat gctagtatcg aacaatttaa gctggtttat ttataaatat gacgaacttg
     9661 ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcact gaacagcgcc
     9721 ctacgaataa gcctaaaaat tgccaacatg cggtacaagt ggctaatgcg agtattcgta
     9781 cgcctgataa taagatcata ttagagaact tgaactttca tgtttcgcca ggcaaatggc
     9841 tattactgaa aggctactct ggtgcgggaa aaaccacact gcttaaaaca ttatcccact
     9901 gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat gtgtcacaaa
     9961 caccgttaat caaaagcggc ttactgaaag agattatttg taaagcactt cccctgcccg
    10021 tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa ttggctgcgc
    10081 gtattcatga ccacgatcgc tggggagata ttctttccag cggcgaaaaa caacgtatcg
    10141 ccctggcacg attaatttta cgacgtccga aatggatatt tcttgacgaa actacctctc
    10201 atcttgagga acaagaggct atacgcttac tgcgtttagt gcgtgaaaaa ctacccacaa
    10261 gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggctgat gatatttgtg
    10321 acattagcac ggttttataa caacaataaa agccctgctg tacgg
//
LOCUS       DAJTVJ010000144        10197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000144 DAJTVJ010000000
VERSION     DAJTVJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.144"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..902)
                     /gene="cra"
                     /locus_tag="OO448_003602"
     CDS             complement(<1..902)
                     /gene="cra"
                     /locus_tag="OO448_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6338718.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVL"
     gene            complement(984..1070)
                     /locus_tag="OO448_003603"
     CDS             complement(984..1070)
                     /locus_tag="OO448_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6338719.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1082..1573)
                     /gene="ilvN"
                     /locus_tag="OO448_003604"
     CDS             complement(1082..1573)
                     /gene="ilvN"
                     /locus_tag="OO448_003604"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6338720.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1576..3300)
                     /gene="ilvI"
                     /locus_tag="OO448_003605"
     CDS             complement(1576..3300)
                     /gene="ilvI"
                     /locus_tag="OO448_003605"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6338721.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3419..3463
                     /locus_tag="OO448_003606"
     CDS             3419..3463
                     /locus_tag="OO448_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338722.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3618..4562)
                     /gene="leuO"
                     /locus_tag="OO448_003607"
     CDS             complement(3618..4562)
                     /gene="leuO"
                     /locus_tag="OO448_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6338723.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5222..5308
                     /gene="leuL"
                     /locus_tag="OO448_003608"
     CDS             5222..5308
                     /gene="leuL"
                     /locus_tag="OO448_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6338724.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5401..6972
                     /gene="leuA"
                     /locus_tag="OO448_003609"
     CDS             5401..6972
                     /gene="leuA"
                     /locus_tag="OO448_003609"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6338725.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            6972..8063
                     /gene="leuB"
                     /locus_tag="OO448_003610"
     CDS             6972..8063
                     /gene="leuB"
                     /locus_tag="OO448_003610"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6338726.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8066..9466
                     /gene="leuC"
                     /locus_tag="OO448_003611"
     CDS             8066..9466
                     /gene="leuC"
                     /locus_tag="OO448_003611"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6338727.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFANIRNIK"
     gene            9477..10082
                     /gene="leuD"
                     /locus_tag="OO448_003612"
     CDS             9477..10082
                     /gene="leuD"
                     /locus_tag="OO448_003612"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6338728.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2683 a         2680 c         2482 g         2352 t
ORIGIN      
        1 agcacacgct ctgcgacatc gcggtgacgt tgagccactg ccagcaccgg gcactgtaag
       61 aagtcgagca gttcgttatc gccaaaggtg gcaattgcca ggtcagaagg cagtttacca
      121 tcgcgacgta gcgtgacatc catcacccct tgcaacaacg caaacgacgt tgtgaacagc
      181 gcctgcggca tcggatgcgt ttccagccat ttttcgaata actgggcagc cacctcccgc
      241 tcatagctgt tggcatacag gaaatgcact tcgcgcggat catctttcca ggcagtacgg
      301 aaaccttgtt cacgcaggaa gctgacagaa agctccggca gcgcgccaag ataaagcacc
      361 gtctcggcgg gaaacttacg taactcttcc gccagcattt cggcatcatc ctgatcggca
      421 ccaaccacgc tggtgaagtg ttcacgatcg agggcgcggt ccagcgcgac aatcgggaac
      481 gggtcgttag cccagcgttg ataaaaagga tgctcaggag gcaacgacgt cgaaacaata
      541 atggcatcga cctgacgctg taaaaggtgc tcaatacagc gcatttcgtt gtctggctga
      601 tcttctgagc aggcaattag cagttgataa ccccgttgcc gcgcctggcg ttcaagatag
      661 ttagcgatgc gggtatagct ggtgttctcc agatcgggga tcacaagacc aatagaacgt
      721 gtgcgtccag cacgaagccc agctgccacg gcgttcgggt ggtaattgtg ctcacgcacc
      781 acagccatga ctttttcaac ggttttgtcg ctcacacggt attgcttcgc tttgccgtta
      841 ataacatagc ttgcagtggt ccgcgacact cccgccagcc gagcgatttc atccagtttc
      901 acaattgccc cttgcgtaaa atgtaaaaac catagccatt gtactggtat gggttaaatt
      961 cattaacatc taagcgcata aaattaactg cggcaactgc ttttattcca ggttcccact
     1021 gatttcgcaa aaaaagccca acgtgacatg ttgggctgca aattgcgcat tcagatcatt
     1081 ctcaacgcat tattttatcg ccgcgcgaaa gtccgaccac accagaacga gcaacttcca
     1141 caattttcgc cacctcgcga atcgatgcta aaaatgcatc aagcttatcg ctggtgcctg
     1201 ctaattgaac ggtataaagc gagggtgtga catcgataat ttgcccacgg aatatttccg
     1261 tattacgttt cacttcgtca cgcccgtaac cgctggcctg aattttcacc agcatgattt
     1321 cccgctcaac atgcgcgccc tgccccaact cactcacgcg caagacatcg accagcttgt
     1381 gtaattgctt ttcgatctgc tcaagtactt tttcatcgcc cacggtctgg atggtcatac
     1441 gcgataatgt cggatcatcg gttgacgcaa cggtcaggct ttcaatgttg tagccacgct
     1501 gggaaaaaag gccaatcacg cgggataacg cgcctgattc attttcgagt aagactgata
     1561 atatccggcg cataatcagg ttctctccgt tttgcttaac cacatttcat ccattccgcc
     1621 cccgcgaatc tgcatcgggt agacgtgctc gctgccatcg acggtaacat caacaaacac
     1681 caggcgatta ttgtgcacct gttccagcgc ctcgctaagt ttgctttcca gctcatgcgg
     1741 atgagaaatc tggatcccga cgtgaccata ggcttccgcc agacggacga aatcgggtag
     1801 cgattgcata taagattgtg aatgacggcc ggaatagatc atgtcctgcc actgcttcac
     1861 catccccaga tagcggttat tgagattcac caccagtacg ggcaactcgt attgcaacgc
     1921 ggtagacagt tcctggatgt tcatctgaat actgccgtcg ccagtgacgc aaaccacggt
     1981 ttcttctggc aacgccattt tgacgcccag tgccgcaggt aaaccaaaac ccatcgtgcc
     2041 gaggccaccg gaattgatcc agcgacgcgg tttgtcgaat ggataataaa gtgcagcaaa
     2101 catctggtgc tgcccgacat cggacgtcac gtaagcgtct cccttcgtca accgccaaag
     2161 agtctcgatc accgcctgcg gtttaatctt ttcactgtga gtgtcatatt tcaggcactg
     2221 acgagcgcgc cactgttcaa tttgctgcca ccagtcgcgg atctcatcca gtggttgatg
     2281 ggcggattct tgcgacaaga gttcaagcat ttgttcgagg acctggcgag catcccccac
     2341 aatcgggata tccgcagtca cggttttaga aatggaagta ggatcaatat cgatatgcag
     2401 aacagtggca tttgggcagt actttgccag attgttcgtc gttcggtcat caaatcgtac
     2461 cccgacggcg aaaatcacat ccgcgttatg catcgtcata ttggcttcgt aggtaccgtg
     2521 cattcccagc atgcccagtg cctgacgatg cgttgccgga aacgctccca gccccatcaa
     2581 tgaggaaaca acgggcagat tcaacgcctc caccgtttct ttcaactgct gatggcagcc
     2641 cgccgtgatt gccccaccgc ctacgtagac aaccggtttt tttgccgcta ccagcgtttg
     2701 cagagcgcgc ttaatttgcc ctttatgtcc ggtagtagtg ggattgtaag aacgcatact
     2761 gaccgactcc ggccagacat agggtaattt gttcgccgga ttaagaatat ctttcggtaa
     2821 atcaacgact actggtccag gacgaccact tgccgccagc cagaaagcct ttttcagcac
     2881 ctgcggaatg tcttccgttt gcttaaccag aaaactgtgt ttaaccactg gtcgcgaaat
     2941 ccccaccatg tcgcactcct gaaaggcatc gtaacctatc aacgaggtcg ctacctgccc
     3001 ggaaaggaca actaatggaa tggaatccat ataagcggtg gcgatgccag taatcgcatt
     3061 ggtcgcccct ggacccgacg ttaccagcac gacgccgact tccccggtcg cgcgcgccag
     3121 accatcggcc atatgcactg ccgcctgctc atgacgaact aaaacatgat cgataccacc
     3181 cacggtatgc aatgcatcgt aaatatcaag gactgcgcct ccgggataac cgaatacttg
     3241 tttaacgccc tgatcgataa gcgatcggac gaccatctcg gctccagaca acatctccat
     3301 ggcctgcctc actgtttgac ggaaaaaatg tgtaaaacac attaaccgtc cgatcttgct
     3361 taagcaattg gcagccagag acataaacgc agggtaataa gaataaacag gaggaaaggt
     3421 gaaaagaaac gataaaatcc tccattacga aggattaaat tgataataaa tcggctgaat
     3481 cccacaactt acagcaattt ctctattttt ttaattaatc ctaaaataga gagcccacac
     3541 attcagaata aaattctaca ttcaataaaa aatacagaat aaaccagaca ttcatgtctg
     3601 acctattctg cattcagtta gcgtttgcaa attgagacta attgctcttc catccactga
     3661 tggcctttat cgcgcccggc agcttcatgc caggagagat aacaggttct gctattttgt
     3721 tttaacggca gcggtaatac ctgtaattct aaggattcag cgaactcttc agccagccaa
     3781 cgcggcgcaa tagcgaccaa atgcgtttgc gacaccacgc taagtacgct catcattgcc
     3841 atgccctgat acgcgatact ggcttgctta tctaccgtgt cataccaagg ttgactaaat
     3901 gacgcgaaac gatcgagcga aaccgccgca tgttgttcgt tataaacatc atgtttcagt
     3961 aacgggccct taattgttgg atgatttttg ctggctacca gcaccatttc atctttaaat
     4021 aatggcacgc tggtaaattc aggacgatgg aagtcttcat aactaatcac aaactccgtt
     4081 tcctgataac gcagctgatg ttcagtgttc tgatttaatg aagacttgaa cataacatgt
     4141 atatttggcg caatctgctc aatgtgatta taaatctgcg aggtcagaat gctgtctaac
     4201 gggctgcaaa cacaaagatg aaatacacgt tcactgctcg cgggttcaaa acctgaacca
     4261 ggcaattcat tttgtactag ttgcaatgcc tgacgaactg agccaaaaag ttgaaatgcg
     4321 cgagcagtcg gttgaatacc acggccataa cgaacaaaaa gctcgtcatt aaacatcacc
     4381 ttcaggcgtg caacagcgtt actgaccgca ggttgcgaca tacccagaac atgagcggca
     4441 cgggtaatgt tttgctcctg catcacggca tcgaaaacgg ttaataagtt gagatcgacc
     4501 atgcgtagct gtggcttgct taactccgcc gtctctgaat gatctgtttg tacctctggc
     4561 atacttaact ccactgtcac acttaactcc ctttccctta ttggaatgca gagaaataat
     4621 cctgaagaat atgatttatc atgcatataa aataagaaaa agcaaaatga gtaaaattcg
     4681 gaaaacataa agatacgggt tctgataaaa acagaatcca ttgccgtgga atgagtcatt
     4741 taccacagca taagaatcca taatgttttt gttgcgaaaa caatctaatc ataactaccg
     4801 cgaatactca atcatctaca aaatggatta aatgtgctta tttaataatt aatttatata
     4861 tttaatgcat taattcttaa cattaattga tcaataatat tcaccaaatc aatatcaaaa
     4921 aaaatcgcaa aacatataat tcaatacaaa tcatcaggat aggttttgca acgcgtgcat
     4981 tttgtcccct ttttcctcgt tgattagatg caaaaattta tgctgaaata tgtcaaccga
     5041 tgaaaagcgt cggtagttaa gcagaaatta atatcgctta ctttaaccac cgcagcacaa
     5101 ttagctaatt ttacggatgc agaactcacg ctggcgggac gtttttattg cgtcagggtt
     5161 gacatccgtt tttgtatcca gtaactctaa aagcatatcg cattcatctg gagctgattt
     5221 aatgactcac atcgttcgct ttatcggtct actactacta aacgcatctt ctttgcgcgg
     5281 tagacgagtg agcggcatcc agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc
     5341 attgcgcggg tttttttatg cccgaagcga ggcgctctaa aagagacaag gacctaaacc
     5401 atgagccagc aagtcattat tttcgatacc acattgcgcg acggtgaaca ggcgttacag
     5461 gcaagcttga gtgtgaaaga aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt
     5521 gacgtgatgg aagtcggttt ccccgtctct tcgccgggcg attttgaatc ggtgcaaacc
     5581 atcgcccgcc aggttaaaaa cagccgcgta tgtgcgttag ctcgctgcgt ggaaaaagat
     5641 atcgacgtgg cggccgaatc cctgaaagtc gccgaagcct tccgtattca tacctttatt
     5701 gctacctccc caatgcacat cgccaccaag ctgcgcagca ccctggacga ggtgatcgaa
     5761 cgcgctatct atatggtgaa acgcgcccgt aattacaccg atgatgttga attttcttgc
     5821 gaagatgccg ggcgtacacc cattgccgat ctggcgcgag tggtcgaagc ggcgattaat
     5881 gccggtgcca ccaccatcaa cattccggac accgtgggct acaccatgcc gtttgagttc
     5941 gccggaatca tcagcggcct gtatgaacgc gtacctaaca tcgacaaagc cattatttcc
     6001 gtacataccc acgacgattt aggcctggcg gtcggcaact cactggcggc ggtacatgcc
     6061 ggtgcacgcc aggtggaagg tgcaatgaac gggatcggcg agcgtgccgg taactgttcg
     6121 ctggaagaag tcatcatggc gattaaagtt cgtaaggata ttctcaacgt ccacaccgcc
     6181 attaatcacc aggagatatg gcgcaccagc cagttagtta gccagatttg taatatgccg
     6241 atcccggcaa acaaagccat tgttggcagc agcgcattcg ctcactcctc cggtatccac
     6301 caggatggcg tgctgaaaaa ccgcgaaaac tacgaaatca tgacaccaga atctattggt
     6361 ctgaaccaaa tccagctgaa tctgacctct cgttcggggc gtgcggcggt gaaacatcgc
     6421 atggatgaga tggggtataa agaaagtgaa tataatttag acaatttgta cgatgctttc
     6481 ctgaagctgg cggacaaaaa aggtcaggtg tttgattacg atctggaggc gctggccttc
     6541 atcggtaagc agcaagaaga gccggagcat ttccgtctgg attacttcag cgtgcagtct
     6601 ggctctaacg atatcgccac cgccgccgtc aaactggcct gtggcgaaga agtcaaagca
     6661 gaagccgcca acggtaacgg tccggtcgat gccgtctatc aggcaattaa ccgcatcact
     6721 gactataacg tcgaactggt gaaatacagc ctgaccgcca aaggtcacgg taaagatgcg
     6781 ctgggtcagg tggatattgt cgccaactac aacggtcgcc gcttccacgg cgtcggcctg
     6841 gccaccgata ttgtcgagtc ctccgccaaa gccatggtgc acgtactgaa caatatctgg
     6901 cgtgccgcag aagtcgaaaa agagttgcaa cgcaaagctc aacacaacga aaacaacaag
     6961 gaaaccgtgt gatgtcgaag aattaccata ttgccgtatt gccgggggac ggtattggtc
     7021 cggaagtgat gacccaggcg ctgaaagtgc tgaatgccgt gcgcaaccgc tttgcgatgc
     7081 gcatcaccac cagccattac gatgtaggcg gcgcagccat tgataaccac gggcaaccac
     7141 tgccgcctgc gacggttgaa ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag
     7201 gcggcccgaa gtgggaacat ttaccaccag accagcaacc agaacgcggc gcgctgctgc
     7261 ctctgcgtaa gcgcttcaaa ttattcagca acctgcgccc ggcaaaactg tatcaggggc
     7321 tggaagcatt ctgtccgctg cgtgcagaca ttgccgctaa cggcttcgac atcctgtgcg
     7381 tgcgcgaact gaccggcggc atctatttcg gtcagccaaa aggccgcgaa ggtagcggac
     7441 aatatgaaaa agcgtttgat accgaggtgt atcaccgttt tgagatcgag cgtattgccc
     7501 gcatcgcgtt tgaatctgct cgtaaacgtc gccacaaagt cacctcaatc gacaaagcta
     7561 acgtgctgca atcctctatt ttatggcgtg aaatcgtcaa cgagatcgcc acggaatacc
     7621 cggatgtcga actggcgcat atgtacatcg acaacgccac catgcagctg attaaagatc
     7681 catcacagtt tgacgttctg ctgtgctcca acctgtttgg cgacattctg tctgacgagt
     7741 gcgcaatgat cactggctcg atggggatgt tgccttccgc cagcctgaac gagcaaggtt
     7801 ttggtctgta tgaaccggca ggcggctcag caccagatat cgcaggcaaa aacatcgcca
     7861 acccgattgc gcaaattctg tcgctggcac tgctgctgcg ctacagcctg gatgccgatg
     7921 atgcggcttg cgccattgaa cgcgccatta accgcgcatt agaagaaggc attcgcaccg
     7981 gggatttagc ccgtggcgct gccgccgtta gtaccgatga aatgggcgat atcattgccc
     8041 gctatgtggc agaaggggtg taatcatggc taaaacgtta tacgaaaaat tattcgacgc
     8101 tcacgtagtg tacgaagccg aaaacgaaac cccgctgtta tatatcgacc gccacctggt
     8161 gcatgaagtg acctcaccgc aggcgttcaa tggtctgcgc gcccacggtc gcccggtacg
     8221 tcagccgggc aaaaccttcg ccactatgga tcacaacgtc tctacccaga cgaaagacat
     8281 taatgcctgt ggtgaaatgg cgcgcatcca gatgcaggag ctgattaaaa actgcaaaga
     8341 atttggcgtc gaactgtatg acctgaatca cccgtatcag gggatcgtcc acgtaatggg
     8401 gccggaacag ggcgtcacct tgccggggat gaccattgtc tgcggcgact cgcataccgc
     8461 cacccacggc gcgtttggcg cactggcctt tggtatcggc acttccgaag ttgaacacgt
     8521 actggcaacg caaaccctga aacagggccg cgcaaaaacc atgaaaattg aagtccaggg
     8581 caaagccgcg ccgggcatta ccgcaaaaga tatcgtgctg gcaattatcg gtaaaaccgg
     8641 tagcgcaggc ggcaccgggc atgtggtgga gttttgcggc gaagcaatcc gtgatttaag
     8701 catggaaggt cgtatgaccc tgtgcaatat ggcaatcgaa atgggcgcaa aaaccggtct
     8761 ggttgcaccg gacgaaacca cctttaacta tgtcaaaggc cgtttgcatg cgccgaaagg
     8821 caaagatttc gacgacgccg ttgcctactg gaaaaccctg caaaccgacg aaggcgcaac
     8881 tttcgatacc gttgtcactc tgcaagcaga agaaatttca ccgcaggtca cctggggcac
     8941 caatccaggc caggtgattt ccgtgaacga caatattccc gatccggctt cgtttgccga
     9001 tccggttaaa cgcgcgtcgg cagaaaaagc gctggcctat atggggctga aaccgggcat
     9061 tccgctgacc gaagtggcta tcgacaaagt gtttatcggt tcctgtacca actcgcgcat
     9121 tgaagattta cgcgcggcag cggagatcgc caaagggcga aaagtcgcgc caggcgtgca
     9181 ggcactggtg gttcccggct ctggcccggt aaaagcccag gcggaagcgg aaggtctgga
     9241 taaaatcttt attgaagccg gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc
     9301 gatgaacaac gaccgtctga atccgggcga acgttgtgca tccaccagca accgtaactt
     9361 tgaaggccgc caggggcgcg gcgggcgcac gcatctggtc agcccggcaa tggctgccgc
     9421 tgctgctgtg accggacatt tcgccaacat tcgcaacatt aaataaggag cacaccatgg
     9481 cagagaaatt tatcaaacac acaggcctgg tggttccgct ggatgccgcc aatgtcgata
     9541 ccgatgcaat catcccgaaa cagtttttgc agaaagtgac ccgtacgggt tttggcgcgc
     9601 atctgtttaa cgactggcgt tttctcgatg aaaaaggcca acagccaaac ccggacttcg
     9661 tgctgaactt cccgcagtat cagggcgctt ccattttgct ggcacgagaa aacttcggct
     9721 gtggctcctc gcgtgagcac gcgccctggg cattgaccga ctacggcttt aaagtggtga
     9781 ttgcgccgag ttttgctgac atcttctacg gcaatagctt taacaaccag ctgctgccgg
     9841 tgaaattaag cgatgcagaa gtggacgaac tgtttgcgct ggtgaaagcc aatccgggga
     9901 tccatttcga cgtggatctg gaagcgcaag aggtgaaagc gggagagaaa acctatcgct
     9961 ttaccatcga tgccttccgc cgccactgca tgatgaacgg tctggacagt attgggctta
    10021 ccttgcagca cgacgacgcc attgccgctt atgaagcaaa acaacctgcg tttatgaatt
    10081 aatccccttg cccggtcaaa tgaccgggct ttccgctatc gtccacgtca tcaaacgtct
    10141 ttaacctttg cggttaaaaa taatgcgaca acagaaataa ccgcaattac ccagtag
//
LOCUS       DAJTVJ010000145        10157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000145 DAJTVJ010000000
VERSION     DAJTVJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.145"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..353
                     /locus_tag="OO448_003613"
     CDS             <1..353
                     /locus_tag="OO448_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR6338729.1"
                     /translation="RRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLN
                     LVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRL
                     EDGKFRPLRMPGKP"
     gene            386..796
                     /gene="istB"
                     /locus_tag="OO448_003614"
                     /pseudo
     CDS             386..796
                     /gene="istB"
                     /locus_tag="OO448_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
     gene            909..2117
                     /locus_tag="OO448_003615"
     CDS             909..2117
                     /locus_tag="OO448_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR6338730.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKLSVEAALNAEMT
                     HHLGYEKNQSRPGANSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTRITG
                     MDNQILSLYAKGMTTREIATAFKELYDADVSPALISKVTDAVMEQVVEWQNRPLDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRD
                     LKAIYQAPTEEAGQQALEAFAAAWDSRYPQISRSWQANWPNLATFFAYPMDIRKVVYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQKWTMPLKDWRMAMSRFII
                     EFGDRLDGHF"
     gene            complement(2329..2610)
                     /locus_tag="OO448_003616"
     CDS             complement(2329..2610)
                     /locus_tag="OO448_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6338731.1"
                     /translation="MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(2607..2876)
                     /locus_tag="OO448_003617"
     CDS             complement(2607..2876)
                     /locus_tag="OO448_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338732.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(2931..3089)
                     /locus_tag="OO448_003618"
     CDS             complement(2931..3089)
                     /locus_tag="OO448_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338733.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISW"
     gene            complement(3784..4815)
                     /locus_tag="OO448_003619"
     CDS             complement(3784..4815)
                     /locus_tag="OO448_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6338734.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
     gene            5788..6051
                     /locus_tag="OO448_003620"
     CDS             5788..6051
                     /locus_tag="OO448_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6338735.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            5969..6256
                     /locus_tag="OO448_003621"
     CDS             5969..6256
                     /locus_tag="OO448_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HCR6338736.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            6698..7231
                     /locus_tag="OO448_003622"
     CDS             6698..7231
                     /locus_tag="OO448_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HCR6338737.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            7485..8168
                     /locus_tag="OO448_003623"
     CDS             7485..8168
                     /locus_tag="OO448_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HCR6338738.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLEAAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            8182..8748
                     /locus_tag="OO448_003624"
     CDS             8182..8748
                     /locus_tag="OO448_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR6338739.1"
                     /translation="MKLQYRIPLAIYSVWCIIAILLCKDGLYQMNIWTLVEILGMIAL
                     PLLVRPFFILLRIIFRKNSFKTKDMSDNHTVCVFLSALADTSSKKAIKKHFKQLLEKL
                     PPLLRQKKRIYMKSHLLTEARTQKLICSLRRKGLDVTAERRESAMNSVMFRILIVSRI
                     LSQWKVPHINPRCGIVILTLKNDSGNRE"
     gene            9016..9270
                     /locus_tag="OO448_003625"
     CDS             9016..9270
                     /locus_tag="OO448_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HCR6338740.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNC
                     LVVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
     gene            9313..9765
                     /locus_tag="OO448_003626"
     CDS             9313..9765
                     /locus_tag="OO448_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HCR6338741.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYGAIITVVFIESSEQARIRRQQYLQQYKMPNEIHENVVIENSIISRETTDDESDI
                     NTLYLDSFKSKNNCKSFETNSNLLDKNGKPVEIMVCDVQELHFFVPKN"
     gene            complement(9801..>10157)
                     /locus_tag="OO448_003627"
     CDS             complement(9801..>10157)
                     /locus_tag="OO448_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6338742.1"
                     /translation="FGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSP
                     ESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYL
                     RQQASNGLSDNRCLEI"
BASE COUNT         2549 a         2571 c         2430 g         2607 t
ORIGIN      
        1 gccgccgcgt gcatgccggc ctgaacaaag gtgaggcgcg caactcgctg gccagggcgg
       61 tgttcttcaa ccgccttggg gaaatcaggg atcggagctt cgagcagcag cgctaccggg
      121 ccagcggcct caacctggtg acggcggcta tcgtgctgtg gaacacggtg tacctggaac
      181 gcgccaccca ggggttggtc gaggccggca agccggtgga cggcgagctg ctgcaattcc
      241 tgtcgccgct gggctgggag cacatcaacc taaccggcga ttacgtctgg cggcagagcc
      301 gcagactgga agacgggaag tttcggccct tacggatgcc cggaaaacct tagcgtacga
      361 ttttttccga attctgcggg ctccccatga agccgtccgt gcaggtatca aagttcgctt
      421 cacaacagca gcagatctgt tacttcagtt atctacggca caacgtcagg gccgttataa
      481 aacgacgctt cagcgtggag taatggcccc ccgcctgctc atcattgatg aaataggcta
      541 tctgccgttc agtcaggaag aagcaaaact gttcttccag gtcattgcta aacgttacga
      601 aaagagcgca atgatcctga catccaatct gccgttcggg cagtgggatc aaacgttcgc
      661 cggtgatgca gcactgacct cagcgatgct ggaccgtatc ttacaccact cacatgtcgt
      721 tcaaatcaaa ggagaaagct atcgactcag acagaaacga aaagccgggg ttatagcaga
      781 agctaatcct gagtaaaacg gtggatcaat attgggccgt tggtggagat ataagtgagc
      841 ctgtatatag atttgtgtaa ttgcctgatt ttgatatgtt caatccagca tcaaatgaag
      901 gttaatttat ggacgaaaaa cagttacagg ctctggctaa cgaactggcc aaaaacctca
      961 aaacccctga agacctcagt cagtttgatc ggctgctgaa aaagctcagc gttgaagccg
     1021 ctctcaatgc tgagatgaca caccatcttg ggtatgagaa aaatcagtcc agaccaggag
     1081 ctaactcccg caacggttat tccacaaaga ccgttatcac aggcgatggt ccactggaac
     1141 tgcgtactcc gcgcgatcgt gacggtacct tcgaaccgca actggtaaag aaaaatcaga
     1201 cccgtattac cgggatggat aaccagatcc tctcgttgta tgccaaaggg atgaccaccc
     1261 gtgagatagc tactgcgttc aaagaactgt atgacgcaga tgtttcaccg gcactgatat
     1321 caaaagtcac cgatgccgtg atggagcagg ttgtagaatg gcaaaacaga ccactggatg
     1381 ctgtttaccc cattgtttat cttgactgta tcgtcctgaa agttcggcag gacagtcgcg
     1441 tcatcaacaa atcggtgttc ctggcactgg gcatcaatat cgaaggtcag aaagaactgc
     1501 tgggtatgtg gctggccgaa aatgaagggg cgaagttctg gctcaatgtg ctgactgaac
     1561 tgaaaaaccg cggtctgaac gatatcctca tcgcctgtgt ggatggcctg aaaggcttcc
     1621 cggatgccat caacacagta tatccgaagg cccgcatcca gttatgcatc gtgcatatgg
     1681 tgcgcaacag cctgcgcttc gtgtcatgga aggactacaa agccgtcact cgcgacctga
     1741 aagcgattta tcaggctccc acggaagagg caggccagca ggcactggaa gcgttcgctg
     1801 cggcctggga cagtcgctat cctcagataa gccgaagctg gcaggctaac tggccgaatc
     1861 ttgccacgtt cttcgcttat ccaatggaca tccgcaaagt ggtctatacg acgaatgcca
     1921 tcgagtcgct aaacagcgtg atccgccatg cgatcaaaaa gcgtaaagtg ttcccgacag
     1981 acgactcggt gaaaaaagtg gtgtggctgg caatccagtc tgcgtcccag aaatggacga
     2041 tgccgttgaa ggactggcga atggcaatga gccgctttat tatcgagttc ggtgaccgcc
     2101 tggacggtca cttctgagaa aaggcattta cacagaatct taaacaggct catataagtg
     2161 gatcactttt catccgtcgt tgacaggggc tggtttcatt ctgaattcat cgcgcaaacg
     2221 tcctttcgag aaacacctgt cttcggcaat cgcctgcgca cgtctggtgg ccagctcatc
     2281 attattcatg gttattgcat ctcctttcct tatatccgga cagcccctct atggccactt
     2341 ctgtgcagta tgcaacacgc gcaggatata cacttttccc cactggctgt cgacctcata
     2401 aaccagaaca aaatgcgggt gaataaccag ttcccgggta ccttccacac gaccgttgcg
     2461 gcctttatac ggctgcccgg ccaggctgct ggctgaataa ccaatccgtt cgtccagttc
     2521 aatggcggcc atcaggttct gctccgcaat gtacttacga atgcgcttcc tgtcctggct
     2581 ggccagcatg gtccagaata tctccatcat ttaccggcaa ccttcctgct catttcatcc
     2641 cgccatgcag cagcggaaga ttcgaccatg tcatggggaa gcacatcgcc gcgctcgagt
     2701 tgctgacgtc ctgcctcaac ctggtcacgg aaccaggtat tatgctgccg ttcaacggtc
     2761 tggcgcataa aatcccgaat taactgagag ccattacggt ccatgctttt tgcagcggcc
     2821 ataaaagcat ccttcagttc agcgtcgatt cttaaactca tattaacctg tgccatttat
     2881 cacctcgtca ttactacgta tatacacagt atataacgat tcagatatcg tcaccaggat
     2941 atgccgcgcc agcggcatgg aaggcggcac gctgtggtta catgtgatac cggagtaaaa
     3001 ccgccgaagc ccggcgtcag ccggtactga ttgatagatt tcaccttacc catccccagc
     3061 cctgctaagc catacccgct ttcagccatg agagagtttc tgtgcgcggt cggagtggtc
     3121 ccgacgaggg tttacccgaa gtcggggcgt atctccgcgt tagcgggccg tcagggccgc
     3181 ttacgagcgt gtactgagaa catccagcca gaagactgac agcgatgata atgtagttac
     3241 aacattcata attaaaagcg atgttgttcc cgccctttgg gcgggggcgg cctcgctttt
     3301 cagttatgag gggtgggcac ggtgctgcgt ttgtgtggtg tttttattcc tgatgtcttt
     3361 ttgttgtggt ttcagaacgg tgaggttggg gcccgcgcgt cagcgtggga ggtgctttta
     3421 gattctgagc ggcgcggcct tggccgctgc aagcgaaggc ctctttgtat ttgttcacag
     3481 tggtctcaga gataaagatt ttccctgcgg cgaagcaacg caggggatta aataatgggg
     3541 ttaagactat attgcaggtt tgctgtttca ggtttgcagc ttcttaaagt tctttgtttg
     3601 cggatttatg tggataactg aaacaacgtg gccatgttct gatgactgat gcgtggtttt
     3661 atgggcagat atgcccctct gaggggattt aaggtgatta ctgcggattt tgggctgagc
     3721 ggaaccagcg ccgaaagccc cggaggggct ggtgatgatt cagtgctgag ggaatggtgc
     3781 tgtctagtgc ggtggcgggg cagccagcgt cagttcaagc tgacgcaact ccctgatggc
     3841 cattcgcttc agatggtcat cggaacaaaa ataggcttca tccggaggca gacgcttcct
     3901 gagatactct gccatctcat gaatctgctg gtcggcaggc agtttcttca ggcgtctggc
     3961 gcgcttactg gctgctgctt tcgcacgacg gtgtttcagc gcatcctgac tgcgctgcag
     4021 ataccagcgt tttctggccg catgtacgga gatatcttca tcctcacgca gaacaccttc
     4081 ccgaatgagc tgctggcgga tttcactttc acgcagtcgt ttctgctgct gttcgtgaag
     4141 ttttaccatg tcgacgccca gcatctgcca gcctgccggt gttatccaga cgtaacgtgg
     4201 cagacgctgg cgggtttcac ggtcccacag ggtttcctct gaaacaccca gcacaccaaa
     4261 acgtacctgt tcggccagca aacgggaaag gcgggagacc gtcacttcca gttccggaat
     4321 aaccttcccc ttgctgtctt tcgggctgat ttctctggcc agacgggaaa cactcatgcc
     4381 tacggtatgt gtgcccgcat cactgaagct gaccagaacc ggccagacgg catcgaggag
     4441 gcgttgtttt tccggacgga aagcatattt gcgaccggca tacgttcgtt tctgcacgta
     4501 aaaaggatgc agagacatgt gcatgcgcag gctctgctcg ccggtcaccg ggtccttttt
     4561 caccagacgg ttgtaggcgt gcccgagctg cccgctcagc gccttatagc gctcaggacg
     4621 ataccaggac ggattttcgc atttgggttt ggtggagtgc tcaccacgac gacgcttcgt
     4681 gcgcttcctt tccggttcaa ccaggaaagt ggtcgccctt cccgcttcat agtcttccca
     4741 gaaacggatc tgctcttcgg gtgctaactg actccagtgt actgcgttat atggagtatt
     4801 cttcagacct gccacaagct gtcgcttatg gcggagcgat tgtgccgtgt atttatacac
     4861 tgcacagggt tgaatctctg gtacggtttc attatacttt acccgtattc tgtgaggccc
     4921 ccactatctt tcgtcgttcc gccaagttcg taagaaagat agtgggggtt ttccttttat
     4981 ggcttccgga acatcttcag tcttctgact tactgcccgt acatgccatc tctcctgaat
     5041 gccttatggc gcattctgcc gtccggcttt cgccgctttg ctccacagag tgccaccctc
     5101 tgcacagcta ataacagccg aaatataccc gctttacgtt aatttgaaaa gcattacaac
     5161 gatctcttct cttccagtgc ctcctcactg atacctctaa taagttaatc ttgacctgcg
     5221 tcaaaaaaat aaaatctatt tttaaaagca gatttttctg ctttagatct ttcttttgga
     5281 ttaaaagatc gacttttgtc ctactcacga tcatttatct tacaaccgat ctacccaaca
     5341 gaataatatt ctgttttaat gcaattaaac gatctaaata acctttaaaa aatcattttg
     5401 tagatcattc gatcgtaaat agaatgattc ttagatgata aaaccatttg ttacacaacc
     5461 cgccaattgt gtaacaacag cctctacaag agcatatttg ttcatatgtt cgtcagaact
     5521 gcggattgtc tggacgaggg tcagacaccg tttcgtgcat atggaccagc atgttcgaac
     5581 gccacaaagc agaagcatca tgcctggatc gcttcggcgc ggagcgttaa gttttgacgc
     5641 ttttttgtat gttttttaag ctgttagttg actcggtgcg ttatactaag taccctttac
     5701 tggtcacagg caaaactgtg atcgggcgta ggaacccgcg tagtcatcgt cggcgcagaa
     5761 gttaatttct tcatttggcg tcgttttttg ccataccata aagacaggaa cttgtatttc
     5821 ttgtcgtcat ctatggtagc tcaggcgggg caggcattag cctggccggt ttccgatgac
     5881 gccggtattc ctacccccgt ctgggctatc accaatgagc gtaggaactc ggtgatagcg
     5941 tttttagttg tcatcggaga cctttgctat gtcacctcaa ctgatacaga aactcccggc
     6001 catcactctg ttggaaggca tgtttcccga gctatcaaca aaccagctta aagtctgcgt
     6061 tttttacgca atgggtgttc cttacgacgc cattgctcag aactgtcggc tttctcccga
     6121 aacggtacga acctacctga agcgaagcct gaagaattta aatctggaag gatatgatgc
     6181 acttcgctct gctgtgctga tgcgaacttt cgttttcatg attagcaata cagctaaaga
     6241 aaacgaaaaa atgtgaacag gtgtccccat taaaggggac actttcatgg aaattgcatt
     6301 ttgcacttta caggcgatcc caaattgata aacataaaca ccaagcacaa gttactttaa
     6361 taaagcaaat cacttcagac ggtgtcccag ttatttgtat ctggctcatg cccgatatat
     6421 ggcgacctta ctctgaactg acgtgctgat gttttatttc gcgtatcaca atactgtgcc
     6481 actgtattgc tgatattagt gcttagccgt tctttatctg tgagttcgcc aaatcaccga
     6541 tacagaacgt actagagtct gctggttata tgcctgtcag aactgcagtt tatccagata
     6601 tgggctggac actgcttcag acatatgatt tagcatattc gcctgacatc gcaaaatcgt
     6661 ccatcatgga tcggttttgc gcggagcgtt gaacttcgtg aatatagaac acctgaataa
     6721 ccggaactgg tatcttgcgc aatataatac agccggcaaa aacagagaat ctttattctc
     6781 atggctgaat gagcaaaacg tcgtaccatg gactccatta ataacaagaa agataagacg
     6841 ggcagactcc aggtgctgtt accgtgaaag aatattcgct atttttccag ggtacttttt
     6901 tattctggct aattttgaca ttcacccagt atcagcatta cgtcgacata gcgccttcat
     6961 tgatttcgtg aagtttggcg gtgaaataaa acccgtcaat aaagatattg ttgatggatt
     7021 aatgaagatt tacccggacc ctgtgctaaa tccgggagcc agggaagagc tgaatgcggc
     7081 ttccagcata tggctgacaa aagcacaata tcagtatctc ctcagaatgg aaaatactct
     7141 ccagcctgag tcgaggatat ccctgctcct ggaactggta tcaaatgccg aacatcacgg
     7201 ctttatggag cgccttgtaa acatcccata gccagtattt cccggccttc gggccgggct
     7261 tttttcaatt caagagaata ttccggagtg aagcctgcac gaagccatcc ccaccatgga
     7321 ctccccctgt cttttgatgg aaccgtgtat ttcttaatgg cgaaagaaat aaaaggcttc
     7381 accccagagt attgattcgt tttatcaaag gccggcccgt gccatcacgt gtggcggaga
     7441 gaacggatac cggtcttctt ttcatctcac agtaggacat tcgcatgacc acgacagaac
     7501 atcccgtgcg ccagttgctc tggtgtgcac tgaatgccct gaaaacggca caggaaaacc
     7561 acaatataac cagtgaaacc ggattacggc attacctgct tgaatggctt tctggtgcca
     7621 gcagacatcc tgagttccgt agtctccctg gcgaaatcat ggcgttaaaa gcattgaccg
     7681 aaaaagatcg caatattccc atcatcggaa cgctgaatac gctctttctc agctctgcca
     7741 ctgtcgggga atgtccgttg tttcgttttc gggctgcact gggtaaatta cagaaagcag
     7801 gctggcgcac atatgtctgc ccatggcctg agagggtgtt caatgaatcc attgaacggg
     7861 cctgttccgg gaaacgtcat ctgctacaac taagtcgcac cgaagagtgt tttctaccga
     7921 caggcgatat gagggcacca gttacgttgc agctgatcac tccactacac cgcaaaacta
     7981 tgcagcttct ggaagcggca gagttaatat tctccgacga aggatttcag gtggtgagag
     8041 gatacgaaca tcaattcggg atcgaacgca gagaaaccct ctttcacacc ttatttatcg
     8101 ggctgcccag cctgccagaa gatgtctggg gtgcaacaca ggagcagacg actatcacat
     8161 tacactgagg aagagcaata catgaaactc caatatcgta ttccattagc tatatatagc
     8221 gtctggtgca ttatagcgat tttgctctgc aaggatggct tgtaccagat gaacatatgg
     8281 acgctagtag agatattggg gatgatagct ctcccgctgt tagtccgtcc gtttttcatt
     8341 ctgcttagaa taatatttag gaaaaatagt tttaaaacca aagacatgtc tgacaatcac
     8401 acagtatgtg ttttcctgag cgcactcgca gatacttcct caaagaaggc gataaaaaaa
     8461 cacttcaagc aattactcga aaagctgccc cccttattac gccagaagaa acgtatctac
     8521 atgaaatccc atcttctgac tgaagccagg acacaaaagc tgatttgctc tctgcgccga
     8581 aaagggctgg atgtaacagc ggaacgaagg gaatccgcca tgaatagcgt catgttccgc
     8641 atcctcatcg tcagcagaat cttatctcag tggaaagtgc ctcacataaa tccccgctgt
     8701 ggtattgtca ttctgaccct gaaaaacgat tctggaaaca gggaataaca gttataagag
     8761 caccacgatt tccgacagga aaacatacat gaaaatcaac gtagatgacc ctggcaaaaa
     8821 gagcttcgct ctcattacgc ctcacgtgcg ttaattcccg acacaccccg tgacccgtct
     8881 tcgcagcctg taccaccctt tctgccgttc ccgggattct ttctgtcact actcgcttgt
     8941 gccgtttttt cgcaaccggt acgtgcagtt acgacggtac agctgcttct tttcccgttc
     9001 cgaaaggagt ctttgatgcc acagcaacat cccggacgac tacaggtact ggtcgtcgac
     9061 acgcactgca aacgcaagct gttttcaacg aaaacacaga cagatcccga tgagcttgcc
     9121 cgacgcttct gcacgccgga taactgcctt gtggtggtac tatgcaacaa ccgatttctg
     9181 ttccgcctgg aacgtgcacc cggtagccac tgccgctggc gcaagggtag ccgctcccgc
     9241 catcagcacc tgcaggactg gctgtcgtaa aaacaacaaa aacaaaagac ctcgtcaaaa
     9301 caggagggga ccatgtcgtt tttatttaaa ttaaccatcg cgattttgat tgtctttgca
     9361 atcctttttc cgattttaat ttcatcagat aacaaactgt ccgcactttt ttggaagata
     9421 ccaaaagatt tctgggtttt cctgattatc atgtacgggg caatcatcac ggtggtgttc
     9481 atagaaagca gtgaacaggc acgcatccgc cgccaacaat atttgcaaca gtacaaaatg
     9541 ccaaatgaga tacatgaaaa tgtggtgata gaaaacagta ttatttcaag agaaacaaca
     9601 gatgacgaat ctgacattaa caccctgtac cttgattcat ttaaaagtaa aaacaactgc
     9661 aaatcttttg aaaccaattc aaatttactg gataaaaatg gtaagcctgt ggaaataatg
     9721 gtgtgtgatg ttcaggaact gcattttttc gttccaaaga attaaacagt taatccccct
     9781 tactctgttt ggatttgccc ctatatttcc agacacctgt tatcacttaa cccattactg
     9841 gcttgctgcc gtagatattc ccgtggcgag cgataaccca gtgcactatg cggatgccat
     9901 tcgttataat gctcgaacgc ctctgcaagg ttctttgctg ccgttaaccc gtctggtttg
     9961 ggcatgacac tgatgtagtc acgctttatc gttttcacga agctctctgc tatgccgtta
    10021 ctctccggac tccgcaccgc cgtgctcttc ggttcaagcc ccaacatccg ggcaaactgc
    10081 cgtgtttcat tagcccggta gcatgaacca ttatccgtca gccactctac tggagacgcc
    10141 ggaagctcgt tgccgaa
//
LOCUS       DAJTVJ010000146         9842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000146 DAJTVJ010000000
VERSION     DAJTVJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.146"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO448_003628"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO448_003628"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6338743.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="OO448_003629"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="OO448_003629"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6338744.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="OO448_003630"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="OO448_003630"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6338745.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="OO448_003631"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="OO448_003631"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6338746.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="OO448_003632"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="OO448_003632"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6338747.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5060..6193
                     /locus_tag="OO448_003633"
     CDS             5060..6193
                     /locus_tag="OO448_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6338748.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="OO448_003634"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="OO448_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6338749.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO448_003635"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO448_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6338750.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OO448_003636"
     CDS             complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OO448_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6338751.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OO448_003637"
     CDS             complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OO448_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6338752.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OO448_003638"
     CDS             complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OO448_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6338753.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8346..9374
                     /gene="epmB"
                     /locus_tag="OO448_003639"
     CDS             8346..9374
                     /gene="epmB"
                     /locus_tag="OO448_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6338754.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9764..>9842
                     /locus_tag="OO448_003640"
     CDS             9764..>9842
                     /locus_tag="OO448_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338755.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKI"
BASE COUNT         2514 a         2500 c         2500 g         2328 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacccggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctac agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttgcca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cgttactcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactcaagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc ggcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcacgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtgggggtg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaatgaac atgatggaag
     2221 gcacgctggt gcagatccgt gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc tccacgccgc gtggcgatgt ggtttatctc gacttgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgaccgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctggttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa tgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgggccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctagag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aagaaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg tctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggcg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tggcggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtatcgttac aatctaacgt atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttactgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 aacccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg
     6361 gcgtgacagt agtgaataat ttcgacgcta aacgctatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagtctgc
     6481 gtgatgacgg cggcctgaat gtcatcaata aaggctataa ccctgacaga gaaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac acgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgtcattgc acttgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca ggcgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacagtaat aacacttggc gtcaaacgac taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gagaattggg gccatccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt gtgactatcg
     7261 caaaaaaaca aacggcacga cataactgtc gtaccgttat tgcttattgc tgcgctttcg
     7321 ttgctgcacc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg
     7381 tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct
     7441 tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa
     7501 taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa
     7561 ttagctggca gcgcgggaga tggagttgcc gagatgtttg atgtcttcgc cgaaaccgcg
     7621 agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt
     7681 catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta
     7741 cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg
     7801 tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt
     7861 acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt
     7921 cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc
     7981 cagcagccat ttagcgttgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt
     8041 ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc
     8101 gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc
     8161 ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa
     8221 ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa
     8281 atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc
     8341 acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac
     8401 ttgccgatgt tgtgaccgat cctggtgaac ttctgcgtct tttgaatata gacgcggacg
     8461 aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat
     8521 ttatcgatcg catggagaaa ggcaatccgg acgatcctat tttgcgtcag gtacttacct
     8581 cgcaagatga gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca
     8641 gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg
     8701 gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg
     8761 gcaacaagcg taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg
     8821 agatgatttt ctccggcggc gatccgctga tggcgaaaga tcacgagctg gactggttgc
     8881 tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga
     8941 ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc
     9001 aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg
     9061 cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg
     9121 gtgtgaacga taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg
     9181 tgatgccgta ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga
     9241 gtgatgacga agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg
     9301 tgccgaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc
     9361 tacgccagca gtaactgatt aaacagggaa gttaaacatt agcctgatgc gctacgctta
     9421 tcaggcctac attgtgcatt gaaattgaat ggatttgcaa tgaactgtag gccggataag
     9481 gcgtttacgc cgcatccggc aagagcaaca aacgcaagta cagcaacctg tcattaaact
     9541 gggaaattta atctttgccc gttgcataac ctttaattat tcatatacat tgctgttgtt
     9601 actctttata tagccaacac caataattaa cctttctttt ctgtttatat agagtgagta
     9661 cggctattta tgttatttta acaactatta ctaccaagcc catttatctg gcttataaat
     9721 taaaattaat tatttaattg agaaatggtt agggagaacc tacatggcca taagcatcaa
     9781 gggtgttaat accggtgtta ttcgcaagag taataatttc attgcactgg cgctgaaaat
     9841 ta
//
LOCUS       DAJTVJ010000147         9821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000147 DAJTVJ010000000
VERSION     DAJTVJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.147"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(39..1034)
                     /gene="gapA"
                     /locus_tag="OO448_003641"
     CDS             complement(39..1034)
                     /gene="gapA"
                     /locus_tag="OO448_003641"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6338756.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1376..1789
                     /gene="msrB"
                     /locus_tag="OO448_003642"
     CDS             1376..1789
                     /gene="msrB"
                     /locus_tag="OO448_003642"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6338757.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1831..2103
                     /locus_tag="OO448_003643"
     CDS             1831..2103
                     /locus_tag="OO448_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6338758.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="OO448_003644"
     CDS             complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="OO448_003644"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6338759.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="OO448_003645"
     CDS             complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="OO448_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6338760.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASARGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="OO448_003646"
     CDS             complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="OO448_003646"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6338761.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6116..6667
                     /locus_tag="OO448_003647"
     CDS             6116..6667
                     /locus_tag="OO448_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6338762.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6784..7827
                     /gene="selD"
                     /locus_tag="OO448_003648"
     CDS             6784..7827
                     /gene="selD"
                     /locus_tag="OO448_003648"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6338763.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7832..9793
                     /gene="topB"
                     /locus_tag="OO448_003649"
     CDS             7832..9793
                     /gene="topB"
                     /locus_tag="OO448_003649"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6338764.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2434 a         2452 c         2618 g         2317 t
ORIGIN      
        1 ccgaagtcgc tctttttaga tcacagtttc atctcaactt atttggagat gtgagcgatc
       61 aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg
      121 aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg
      181 ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct
      241 tcagcagcgg ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga
      301 acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt
      361 ttgccattca gttctggcag tactttacct acagctttag cagcaccggt agaggacggg
      421 atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg
      481 gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag
      541 ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa
      601 acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg
      661 tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca
      721 gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc aacttcgtcc
      781 catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc
      841 agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc
      901 agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca
      961 gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct
     1021 actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa
     1081 ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc
     1141 tgtgcctaag cattacgcga ctgactcgcc tcactcttcc tttgggcttg aaaccacatg
     1201 gggtcggcgc cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt
     1261 taggtgcagc catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat
     1321 gttttgttag aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc
     1381 taataaacct tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac
     1441 gcagaatcat gggacagagc cgccatttac gggtcgttta ctgcataaca agcgtgacgg
     1501 cgtatatcac tgtttgatct gcgatgcccc gctgtttcat tcccaaacca agtatgattc
     1561 cggctgtggt tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa
     1621 agacttgtca catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct
     1681 ggggcatgtc ttccctgacg ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc
     1741 ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc
     1801 agcaacttat tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg
     1861 acgcctgagg tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcccgatggc
     1921 gttgcgttga ccgaggaaca aaaagcgaac tgcctgcaac tggtgatgct gtggcaagct
     1981 cgccataata ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag
     2041 agcaaacagc agttaaaaga agattttggt atctcagcaa agcctattgc tatgtttaag
     2101 tgaatgagtt atctagtatt taagcctgtt attaagggag ggcaaatata cttgttagga
     2161 cgtcgtgttt tagctgcaag taggttaaat gcaacactga caagtattat ctggcctaca
     2221 atgtcattga ggaaatgtag gccagagatg aattcaacta atattacccc tgtgtctctt
     2281 cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgcg
     2341 cactgtcctg gggctggata ttcacgccac gacaaccatc ggtaatcaca tttaccttat
     2401 aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca
     2461 taacgatcaa ttcatcgatt tcatgatcgc gtaaccagtc atcgagcgag gttttctgcc
     2521 gacggccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga
     2581 acaccgctgc gatcgctttt tggttcagta acggatgtaa ttgtgcgcct tcactgttct
     2641 gcacacagtg atctggccag aaggtttgtg gcaaaccgtc gagttggcct ggcgtataag
     2701 gctctacacc gtgctgactg gcaaaactgc cgtgattcgc cgggtgccag tcctgactgg
     2761 cgataaccgc ttcaccgcgc gactggcacc agtcaatcag gcggttagcg acatccaccg
     2821 tactgtcacc ttccggcacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga
     2881 ccagtaacag ggcgcgaggg ggcattacag tctccttaat catccggcgt cagttcgccg
     2941 cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg
     3001 taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgccaat aacgccggca
     3061 tgggcgaggg cgttaccggt ggcataacca cccatattca ctttaccgga catacattgt
     3121 gtcaggttga ccaccacaat accgcgagcg ctggcttctt gtaattcctg caggaaggct
     3181 ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt caccggttgg
     3241 cgcagaaaat tgcgcacgac gtcagcagaa atccctggat aaatcgtcac tacgccaatt
     3301 ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta
     3361 ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac
     3421 gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg
     3481 aaaaagagcg ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc attcagcaga
     3541 ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc
     3601 ggtttaccga gattctcgag catgaacgac agcgcagagg cagtatacgc catcgtgtcg
     3661 gtgccgtgca ggatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca
     3721 atatgctgcc agtcttctgg cgtcatatca gaagaatcca tcagcggcgt atattcatga
     3781 atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt
     3841 agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc
     3901 gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag
     3961 caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg
     4021 gggcgagaca gcgggaagag gattcgtcga cgtaaaaaat aatgtggccg acgatctgtc
     4081 ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt
     4141 ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga
     4201 ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc
     4261 agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg
     4321 ccactgtttc acttttgcca gctctgctgc tttggcgacc gcatcatcga aatccccgag
     4381 actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat
     4441 tttatcaatc tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt
     4501 atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt
     4561 gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc
     4621 cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct
     4681 ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat
     4741 gccgcccatc gatacaacca caggcttacc cgctgcccgg gctgctgcca gttcagcgcg
     4801 aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt
     4861 cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt
     4921 cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc
     4981 accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt
     5041 atcagtctta ctccagccga actctttggt tagagctttt tcgatttcgg cactcgatgc
     5101 cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt
     5161 taaaccctca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga tctgccggtt
     5221 agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc
     5281 gcgggctgcc ggtgacatgt catcacgaat aaacggttca acggcagatt tatacgtacc
     5341 cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc
     5401 gttggtagca aaaccgtgca aatccaccac gccttgcggt gacagccaaa ttttattggc
     5461 gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt
     5521 cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc
     5581 gcctgcgaag tttttcagat ccatcacgat accggtgata ttgcggtcgt ccttcgcctg
     5641 gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc
     5701 aagcagctgg cggcttaatt tactaaaccg ctgagaactg tcgggtttat cgacgatcac
     5761 accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact
     5821 gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac
     5881 catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg
     5941 ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat
     6001 gtggttatcg taatgacaca accggcactt gtcagcagga attgcggact gtgctgtaac
     6061 aaaatccact ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta atcaaatgga
     6121 tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac
     6181 gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat
     6241 gcaaccgtgg catttttttg tgattgaagg ggaagggcgc gagcgtttca gcgccgtact
     6301 ggaacagggg gcgattgctg ccggtagtga tgacaaagct atcgacaaag cccgtaatgc
     6361 gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcgaagaaa atcataaagt
     6421 cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc
     6481 cgttgcccag ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt
     6541 gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac
     6601 gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta
     6661 tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac
     6721 tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg
     6781 tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt
     6841 aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat
     6901 ccgaatttgc ttgtgggtaa tgagacccgc gacgatgcgg cggtgtacga tctgggcaat
     6961 ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat
     7021 tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg
     7081 attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc
     7141 gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt
     7201 cactccatcg atgcgacgga gccgattttt ggtctggcgg taacggggat cgtaccgacc
     7261 gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct gacgaaaccg
     7321 ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgctgaaacc agaacatcag
     7381 ggactagcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc
     7441 gaaggcgtaa aagcgatgac cgacgttacg ggttttggtc tgctgggcca tttgagcgaa
     7501 atgtgtcagg gcgcaggtgt gcaggcgcgc gttgactatg acgcgatccc aaaattgccg
     7561 ggggttgaag agtacattaa gttgggcgca gtgcctggcg gcaccgaacg taactttgcc
     7621 agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatttgct gtgcgatccg
     7681 caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga ggtcaaagct
     7741 acagccgccg agtttggcat tgaactgacg gcaattggcg aactggtgcc agcgcgcggc
     7801 ggtcgtgcca tggttgagat tcgttaattc aatgcggttg tttattgccg aaaaaccgag
     7861 tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat
     7921 cgagtgcggt aatggtcagg tggtgacctg gtgtatcggt cacctgcttg agcaggcgca
     7981 gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattatccc
     8041 ggaaaagtgg caattacagc cccgaccctc cgtgaccaaa caacttaacg tcatcaaacg
     8101 cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaact
     8161 gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca
     8221 gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgctcg accgtcttcg
     8281 gtccaacagt gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg
     8341 gctgtacggc atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca
     8401 gggcgtactt tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga
     8461 tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac
     8521 acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca
     8581 ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag
     8641 tggtcaacca gcaattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct
     8701 gcctttttcg ctttcggcgt tgcagattga agcggcaaaa cgctttggtc tgagcgcgca
     8761 gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg
     8821 ttccgattgt cgctatttgc cagaagaaca ttttgccgga cgccacgcgg tgatgaatgc
     8881 catcagcgtt catgcaccag atctgttgct gcagccagtg gtagatccag atatacgcaa
     8941 ccgctgttgg gatgacaaaa aggtcgatgc acaccacgcc atcatcccga ccgcacggag
     9001 ttcagccatc aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcagta
     9061 cttgatgcag ttctgcccgg atgtggtgtt ccgcaagtgt gttatcgaac tggacattgc
     9121 caaaggcaaa tttgtcgcta aagcgcgttt tcttgctgaa gcaggctggc gcacgctgtt
     9181 aggcagcaaa gagcgcgatg aagaaaacga cggtacgcca ttgcctgtgg tggcgaaagg
     9241 cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca
     9301 ttttaccgat gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa
     9361 agatctgaaa aagatccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg
     9421 gattattgaa ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc
     9481 taccgacgcc ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat
     9541 gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga
     9601 ctttatgcag ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc
     9661 ggtgcggcag tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg ataagaaaaa
     9721 ggctgcaccg cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag tcggaagcgg
     9781 ggcgatagcg taagcgagtg aatctttcgt gctattcgag t
//
LOCUS       DAJTVJ010000148         9724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000148 DAJTVJ010000000
VERSION     DAJTVJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.148"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            668..1621
                     /locus_tag="OO448_003650"
     CDS             668..1621
                     /locus_tag="OO448_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6338765.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEKELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            1621..1917
                     /locus_tag="OO448_003651"
     CDS             1621..1917
                     /locus_tag="OO448_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338766.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            2241..2531
                     /locus_tag="OO448_003652"
     CDS             2241..2531
                     /locus_tag="OO448_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338767.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            2752..2967
                     /locus_tag="OO448_003653"
     CDS             2752..2967
                     /locus_tag="OO448_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338768.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            3114..3419
                     /locus_tag="OO448_003654"
     CDS             3114..3419
                     /locus_tag="OO448_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338769.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            3895..4605
                     /locus_tag="OO448_003655"
     CDS             3895..4605
                     /locus_tag="OO448_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6338770.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            4630..4836
                     /locus_tag="OO448_003656"
     CDS             4630..4836
                     /locus_tag="OO448_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338771.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            4935..7115
                     /locus_tag="OO448_003657"
     CDS             4935..7115
                     /locus_tag="OO448_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6338772.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            7272..8016
                     /locus_tag="OO448_003658"
                     /pseudo
     CDS             7272..8016
                     /locus_tag="OO448_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8197..>9724
                     /gene="istA"
                     /locus_tag="OO448_003659"
     CDS             8197..>9724
                     /gene="istA"
                     /locus_tag="OO448_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCR6338773.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PERLEKACYVALHYNAPDRRFIDNLLRHHRDNVELPLSRQGEQHPAYASEHENLRGPG
                     Y"
BASE COUNT         3070 a         1912 c         2143 g         2599 t
ORIGIN      
        1 cataacgggc ttactattcg ttcaccgttt cctagtgcag tcgttgtttt taaaaaagaa
       61 ccacgtaagc acgaaattac acagacggtt tgctgtggtg acacgctatt accgttaacc
      121 atcattaaca gaggaggctt gagaggaaga cccaccattt acccacctga gacgctcgac
      181 agcttcataa aactatacag gcaaggaaaa cccattaagt gcattgcaga tgctctcaga
      241 atgcctctga gcacgtcata caggattgct caaaggttga gctaaaattc tcataaatat
      301 ggtcatttac aagataggtg attcagatat taagaagtat ccctgtgaac aataaatgac
      361 caatcagtaa ttattctgtg aaataattgt tgcaatttat aatcacatct gttatagttt
      421 ttatatgggg tgataaaaga actcagaaca ttaagaggtt acaacgttgg gaggcgtcag
      481 ggtattgaca gattttcacg gcctcttatt caatcaaggc ttttcctttt gctgttaata
      541 ctcgcgtaat cgctcaggct tctgagttcc tttatcagtc catcttctaa ttaattcact
      601 ccctctgggg ttggtcacta tacgtggtca gccctttctt tgttttattt ttaaaaagga
      661 aaatataatg gaaaaattcc acatgtttga tgaccgtatt tatcagatga ctaatggcgt
      721 taaagctgcc gaccgtatta tttacactgt catgttgaat gaacaaaagt tttatcacga
      781 taaggggcaa tattataccc cttcatatgc tcatcttggt cagtatgctg gaatcacaag
      841 aacatccgtg attgcatgta tgaaacaact tgaggcacat ggtttaatca agaaaactaa
      901 cgttgcaagc aatcagtgtg ttattaaagt ttattcactt gatgaagtac cggaggtttt
      961 aacagagctt acagctaagg aagccagaaa ggcaaagctg gagtctaaag ttactcagtc
     1021 agtcaagaaa gatgacccac tgagtcaaaa ttctttaccc actgagtcaa aattttttac
     1081 tcagtcagtc aaaatctttg acacgtataa taacagtaat aacaagaaag aacttattaa
     1141 taacaataaa gaagatgact ttgaagaaga atattttaag aagggagctt cgctctgtga
     1201 ttcacatgaa tgtgattctc ttggtattga agaggctatt aaagagactg ttaaaacatc
     1261 tgacttagat gaccttgata gtgaagctgc taaagaggtc gttaaaaagg aagataaaat
     1321 ctctgatgtg attgctagta gtgaccataa aaacacacag tgccccgcca ctgatgatga
     1381 attaccggat gttgaccttg atgatgaaga agaccctgaa aaggaattga aacgtgaaca
     1441 gcgtaaagca gaaattaaag aaagattcag aaatgcgaag ctaaagaaca aggttagtga
     1501 cctcgatgac tttgatgata gtgacgatga ttttactttt acaccaaaaa agaaaactca
     1561 caccgagcca aagaaagaaa aagttaaagt cagacgtgtt tatggtggag gaatgtttta
     1621 atgaaaactt ataatggcga gagaaaatac cgtgactatt tactcagacg ttacagcatt
     1681 tctcgtgaag gttacttgat gaaagatact cacggggaag tttaccggat tcgaccgaag
     1741 aaagaaggga gggattatta tttcgtcgat gatgtgacag atttaaagat tgacgctttg
     1801 aagtttgcgg tgctgtatca ttacgattta tgggattcag tccaccagct acgattaaaa
     1861 gacggtgacc ccggtaatct gagggccacc aatattatca agggaaagtg ccggtaaggc
     1921 caccagctat ttatttactg aaatctctat ctcaacacga agaagtttaa gtcgaattgt
     1981 aagctttgtc ttttttgtaa tgactcgatt cataacgcaa aacaccaata gcacaataaa
     2041 taccgcaaag cccaacgagc taaaacgatg agattcaata attgctgtga aaaaatcatg
     2101 cattaatttt ccttttgtga aaggaaacag cacctgtatc agcccctaca accatagtat
     2161 catgtaaatg aggtactgac aatatgttaa atagatgtta agattgtagt atgagtaaga
     2221 gatttaagga gatttttaca atgactcaca cagttacgcg ccttttgcca gaggcatttg
     2281 aacgactggc agcaatcaaa gccaaatatc ccgatatgaa gcacacagcg cttgttgggg
     2341 agattttaaa agagtcggta aaccttagcc ctttcgataa cgttaagggc gtcgcagagg
     2401 gttctaaggc catcagaatc acaacgggcc aactggaact actcaaagca cagtccgcag
     2461 tagcaggttg ctcaatgatt caggtgttaa gcgctaaggt ccatcaactg gcggatagat
     2521 tggaggttta atgtgttata ggttccaata ttccacactt ggccgccacc taatcagaca
     2581 gaatttagca gatgctgaat ttgaagatgt gaccctcaaa gcaaacaaac gattaaaacc
     2641 ctctcaaatc caccagctta aacacgaatt aaagccactg gtaatactcg actataacga
     2701 ccctgaacag cttgagcagg gaattaagct tatttctgaa tggggtgctt gatgaaaact
     2761 atcacagtac aacttcagac taataaagca tttcgttatt ttgaaaactt gcttgagtta
     2821 tacgaaggat ggggaagtat ccacggcaaa gatgatatct atcttcactt gtcagcccct
     2881 aactattctc tgaagactcc ggttaagcaa agctggttaa aagactacgg tcaccagatg
     2941 gggctattag tctcagactt gagctaaaaa cagcaaaaca tatctctcag gctttaccag
     3001 acgcaccaga aacgttttta agcccctaat cagggtaact tagggaatcg ccttaagtgg
     3061 tctaaaaaag gggttaaaac gtcttagaat gcgatacagg aaggatttta aaaatgaatt
     3121 acccaatgca aattatggtt gaccaagaaa ccatgaattg gttgattgac ctgaacaggt
     3181 cgggctttaa tatctatttc atgcattgtt ttgaagcgtc aacgtatatt gacaagcata
     3241 taatcaacga aattaaactt ggtgactatg ttcacaagct ggaagtaaca gacccataca
     3301 ataagcgctt tatcattgaa gagtgcaatc gacttgattt aagccctgag caattattga
     3361 agctgaatgt attcttgacc tatcagaatg aattaaaccc ttacagcgag gtgatgtaat
     3421 gacactcgtt attctctgga tgactttagg gttatttgtt ggttgctggg taggtaatac
     3481 ggtaaataag aattgatgtt accaatctgt taaaaagctg ttgacaaata accttaagca
     3541 tgagatgatg gtaataggga agagcaactc ttcctgaatt aaactattaa tcattctaca
     3601 tcaatgatag ttattctgaa aggtgcaatg cttttcgcta catgaccttt caggttagtc
     3661 tgagaggtca tctcgaaaag tactcttgtt cattcgtttg aatccttttt gaatccttta
     3721 gatgttttaa tttcttcacc ttatgaatgg ccctttataa gaaattatct agggctattc
     3781 agcttttaaa aagcatactt ggcaaaccgc cttttaagtc tcttctaagt ttagataggc
     3841 tgtcaggtgt gctttttaaa aactgaaaca cttccgctta gaagaggaaa agcaatgaca
     3901 aatgatattt acattaatgg caaagtgatt gatagcttca gtgactggtc gccacagact
     3961 gaacatccag ctatcactat tcagcgtaaa gagcatgaca agcgtattga gcttgagcaa
     4021 tcgctaaaga tgtcaccaaa aacaaccatc tttgtgtctc ctgagcctca agagatgcct
     4081 gatatttgca agagtgaagc attacttgag cttgaaaaga aatattacca actgctgaaa
     4141 gaacagcgcc ttagattaga tgacgcttac agcaaagtta ccttgatgca atcagctatt
     4201 gagccgtcag aatttgagat tcaggatgaa ttgtcacaga aaccatttgt ctattaccaa
     4261 tacgaagata atgatggttt tggtactttt caagaaaacg tcgcagccgt gattagtagt
     4321 cttcctgctg gacatcgaat tgtgaagatg gttaaggcat caagaggtgc tggtcagttt
     4381 atttacatga ctaataagag cgatgaagaa ctcgctgaaa ttgctagaca gaacatcttg
     4441 gcatcacgta acaagcaact tgataatgtt aagttgtatc tctcaagaga gcttcaggct
     4501 atgaaagagc ttgtgaaaga atatgaagat gctaagaaga ttgctatgca ggctgatatt
     4561 gagcagctta cgaagatttc tagcaaatat gcaaaggcta tttaattaat aattaaaagg
     4621 tggtggtgaa tgattgattt aacagaacgc tatgagctta ttaaagcaat tagtgaacga
     4681 ttgaaagaac aacccaacaa accaaaacga atcaaatcat tcacctcctc taagcaaaag
     4741 aatagaatca ggaaaatagc taagtggtgt cttgataaat taccaagtga ttgtcagatt
     4801 gttggtgaag atggtaatta ttatctggtt aggtaacaag attggttggg tgaaaatcta
     4861 cgggagagat tatcgagtga tatcggtagt ctttcccttt ttcgtatatg aacacttaaa
     4921 gaggtgaaac atgaatgagc aaattcacag ttgatagttt tatcgttgaa cttagtttca
     4981 gtgaaaacgt tattaagggc ttgcaaagag ttgaaaaggc agctttgcag tcagcacaaa
     5041 gaattgaacg caatttgaat agagcgttta aggtcgatac caagcaactt gacagcaatc
     5101 tgacaagttc tttagggcag cttgaaagga agtttaataa gacctttgac aagattgagc
     5161 agagagcaag aagcacaaaa gcatttcagt ttgccaccag atttaatgac actgttaatc
     5221 ctccaaaaca atcaaggcaa ccacgaatta gcggaaacag agcaatcaca gcagcgcata
     5281 gcgtgaatat gtctaagctt ggtgatatta atccgctgat ggctaaaatg tttaaagcac
     5341 agtattacag cttaagcggt aaggctggta atattggaag tgaaaagttt aacgctgaac
     5401 ttgcaaaatt aaatcaatct cttcgtgaaa cgttgaataa actcaggtca cagacaaaga
     5461 ccgccagcat taacaataca agcgacgcat ttaataatct tgcaagcaat gcgattaaat
     5521 tatctggtgc attttactct gtgatgggtg ctcttaacgc ttacaaggcc attatgaatg
     5581 caggtcttaa acgagattcc gcacagagag ccgctaagtt tgtacttggt gataaagcat
     5641 ctgaagctga ggtatttatt cgaaatttag cagacaagac aggtcttaat atctctgaag
     5701 ggctatcaag ctacgctaag tttgcagcag gtgctcaggg ttctatgtca caggaacaga
     5761 cacaggagct atttggtaat gcaacggcta tgagtcgctt aatggggctt tctaacgatg
     5821 agctaaacgg cattttgaga gcatttgagc agatggcgag caagggcaag attcaggctg
     5881 aggaattacg tgggcagtta ggtgaccgta tggctggtgc atttaagcta ttcgctgaag
     5941 cattgggtat gactgctaca gagcttgatg cagccatgaa aaacgggaag gtactttccg
     6001 ctgatactct ccctaaagtc gctaaacaga tgggcttgat gattgataaa gcgggtggct
     6061 gggctgaagt cactaagagc actcagacag ctttaggtaa gttagcaaac aattgggatg
     6121 atacgatggt gaaaatcttc tcaggttcac aggatgagct aaacggcttc ttatccagcc
     6181 tgagtaacct gcttagtgaa atggggatga gttcttctat cgctggtgat gctatcggtg
     6241 gacttatcga catgcttaaa gctggtgtag atgatattcg cgtattcaat aacaccattg
     6301 aaggctggat tttacagact aagaagttct attacagcct tgatgacact aaacgaaaac
     6361 tacttgatga agtcggtgat ggttttatca acttcgtcaa aggtcttgct atagcgttat
     6421 ctgcaaagac gctattcagt gctactacag gggttatgaa cctgacaaga gcaatcacaa
     6481 cgttaggtac aagagctaat caggtggctg gtcaggtagc tactggtaaa ggtggtggaa
     6541 aactgaaagg tgtagctggt ggtgtaggta gtgctcttgc aattggttat gctgatgacg
     6601 gttatgaaac agcgttagca ttagcttcaa tgattccaca aatcagaggc gtcactcttg
     6661 gtttatatgc attgaagaaa gcactggatt ttatgaatca ggaagtagtc aaaaacgcta
     6721 atatgcatcc gtctggggta ggtgtgggta gtgactttaa tcccgtgtat tctggtgacc
     6781 ctaataaacc gaatatgatt aacgaaggat ggagtagaat ttttagttca atgggtgaat
     6841 tgtttaacaa tgccacaatg aatattgcaa gattcaatca tccagagtta aacaacatta
     6901 agcaagattc agcattaacg agtgcagata ttcagggtct gagggatgaa attagtgctt
     6961 tgagtaagcg tattcaggag ccagtgaaag tttcacttgg tggtcaagtt gtcgttcgac
     7021 cagatgaacc atctttcttt actttcaatc aatcagctaa tgaccagttc acgcaggata
     7081 tgctcttaag ttcaagctat ccagaggaag aatgatttaa cggtgttcta cgtgaataaa
     7141 gcaaagttaa tacccacacc aaaaattatt tttcacgtag ctcacgctta acagagaaaa
     7201 tacatatata tcaaaggctt ggttaaaaat gattaaaata cactaagcct ttgaaatcaa
     7261 aggagaattt aatgatttac actaatcagc ttcgttacag tggcaaagat ggcatctatt
     7321 tccacttaag agacaatgta gatgccttct taaccttatc agcaactgaa aacatggaat
     7381 ttgatagccc tatgcaggtg actacacaga acatgcaatt atgaaggaag cttattatta
     7441 actcgtcagg gtcaattagt agaaaacttc attgacactc tggaaaactg gcgtgaccag
     7501 aagcagatta tttctgttat ctgtaaagat ggaattaaaa ttgacgattc cattattacc
     7561 aactttaaag cctctaaaga tgttactatc tctaacggtc ttcgtatcca gctaactttt
     7621 caggaaatta actttaaagc aatcgtaggt caaactgata tttcagcagc cactggcaaa
     7681 acggctacca caaatgacgg tggtgtgacc agtaagaaga atgcaggtaa cgtgaccact
     7741 gaacgcaata acgcgcctat gctatcgtgc aaagagttaa tgaatattag cgatgtatcc
     7801 ggttacgctc cagctatcag aaatgatatt ctcgatgctc agattaaatg taataaggct
     7861 tggaagctgg actattcaga cttaacagaa gctgaaaaaa ccaacgtcac caatgctaat
     7921 agtaacgctg gtcgagtagc ttcaaagctt aaaaagtact cagtgtgcaa aatggcatct
     7981 gaatcgatcg cagtttggca ttcgaatcga tcatgatttg gcatccatct ccgatcacag
     8041 tttggcatca caatcgatca cgatttggca tacttccgat cattgattgg aattcgccag
     8101 tcactccggg aattaactct tttcaccaca gtctccattg ccgtgtttaa accagtggag
     8161 acgtcaatgt ccaaaaagag aaaatccagg agcactatgg aaacttgttt taagatcctt
     8221 cagctcaagt tcgataagaa gctgactaac cgttgtatcg gactgacgtt acacatcagc
     8281 gcctcaaccg tttttgaggt tcttgcccgc ttcaaagcct cttctctgtc atggccatta
     8341 cctgcggata tatcccatga cacgctggag aaactcattt tcccgcccaa agacacctct
     8401 gcgtcagagc ttgttatgcc tgacatgctg tacttcgata cggaaatgag aaaacctggc
     8461 gtgacgcgcc aactgttgtg gatggaatac aaggcacagg cgggtgacaa agcgatggga
     8521 tactcacact tctgccgttg ctaccggaag tggaaaaaaa cgcgacgcct ctccatgcga
     8581 caggagcatc gtgctggcga aaagctcttt atcgacttct gtggtcctac ggtccctgtt
     8641 attaatccgg acaccggaga gatacgccgc gtggcaatct tcgtggctgt aatgggggcg
     8701 tccaactata cctatgttga agcgtgcgaa ggtcaggaca tgatgtcatg gttaaatgcc
     8761 cacagccgtt gcctgacctt cctgggcggc gtaccgaagc tgctcatccc cgataacctc
     8821 agaagcgcag tgaaaaaagc cgatcgctat gagcctgtca tcaacgacag ttatcaggcg
     8881 ctggctgagc actatggcac ggttattatc ccggcccgcc cgcgcaaacc taaagataaa
     8941 ccaaaagcag aaaacggcgt cctcatcgtc gagcgatggc ttcttgcccg catccgtaat
     9001 gaaaccttcc acacgctcag ggcgctgaat gcacgccttc gggagttgct gacggacatg
     9061 aataaccgac caatgaaggg atacggtaat caaacccgcg cagaacgctt ccgcatgctg
     9121 gatgcaccag cactctctcc gctgcctctg gagccttatg aatacacgga atacaaagcc
     9181 gtgaaggttg gccctgacta ccacgttgaa tatgcccgtc actggtactc cgttccacac
     9241 gaactggtcg gtcagcgtct gtcactgaag gtggggcagt cggtggtaca gctctggcac
     9301 aaggggcagt gtgtggcgca acatccgcgc agcacacatg aatataaaca cacaaccaat
     9361 ccgcttcaca tgccagaacg ccaccggcgg catgggacat ggacaccgga acgccttatc
     9421 gagcagggaa accgtacagg tccgtccaca ggccgggtgg ttgaatccat gctgaaagcc
     9481 aaaccccatc cggagctggc ttaccgtgca gtcctgggtc tgctggcact tcagaaaaaa
     9541 tacggcccgg aacggctgga aaaggcctgt tacgttgccc tgcactataa cgccccggac
     9601 agacgcttta tcgacaatct gctgcgtcat catcgggata acgttgagct tccgttatcc
     9661 cgccagggcg aacaacatcc ggcatatgct tcagagcatg aaaacctgcg tggtcctggc
     9721 tact
//
LOCUS       DAJTVJ010000149         9634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000149 DAJTVJ010000000
VERSION     DAJTVJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.149"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..667)
                     /locus_tag="OO448_003660"
     CDS             complement(<1..667)
                     /locus_tag="OO448_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6338774.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="OO448_003661"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="OO448_003661"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6338775.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="OO448_003662"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="OO448_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6338776.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="OO448_003663"
     CDS             complement(5698..7014)
                     /locus_tag="OO448_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR6338777.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="OO448_003664"
     CDS             complement(7004..8761)
                     /locus_tag="OO448_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6338778.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9634)
                     /locus_tag="OO448_003665"
                     /pseudo
     CDS             complement(8777..9634)
                     /locus_tag="OO448_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2444 a         2624 c         2271 g         2295 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaat
//
LOCUS       DAJTVJ010000150         9567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000150 DAJTVJ010000000
VERSION     DAJTVJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.150"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            90..1346
                     /locus_tag="OO448_003666"
                     /pseudo
     CDS             90..1346
                     /locus_tag="OO448_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1349..2677
                     /gene="ssnA"
                     /locus_tag="OO448_003667"
     CDS             1349..2677
                     /gene="ssnA"
                     /locus_tag="OO448_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCR6338779.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2728..3507
                     /locus_tag="OO448_003668"
     CDS             2728..3507
                     /locus_tag="OO448_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCR6338780.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3504..6443
                     /locus_tag="OO448_003669"
     CDS             3504..6443
                     /locus_tag="OO448_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCR6338781.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6376..6690
                     /locus_tag="OO448_003670"
     CDS             6376..6690
                     /locus_tag="OO448_003670"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR6338782.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6726..7076
                     /gene="ghxQ"
                     /locus_tag="OO448_003671"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6726..7076
                     /gene="ghxQ"
                     /locus_tag="OO448_003671"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7145..7633)
                     /locus_tag="OO448_003672"
     CDS             complement(7145..7633)
                     /locus_tag="OO448_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6338783.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7633..9552)
                     /gene="ygfT"
                     /locus_tag="OO448_003673"
     CDS             complement(7633..9552)
                     /gene="ygfT"
                     /locus_tag="OO448_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCR6338784.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2308 a         2565 c         2491 g         2203 t
ORIGIN      
        1 ctgatcctta actgatcggc attattcctc agttccgtat tcctgcagag ttaattcagc
       61 acgatatcga ttttgttgcc gctcacggcg tgaaatttga gtatggctgc tcacccgatt
      121 taaccgttga acagttaaaa aatcagggct tccactatgt tctgattgcc accggcactg
      181 ataaaaatag cggtgtgaaa ctggcgggcg acaaccaaaa tgtctggaaa tcactcccct
      241 tcctgcgtga atacaacaag ggtacagcgc tcaagctggg caaacatgtg gtcgttgtcg
      301 gggcgggtaa caccgcaatg gactgcgctc gtgcggcgtt acgcgttcca ggcgtagaaa
      361 aagcaacgat cgtttaccgt cgttcactac aagagatgcc cgcatggcgc gaagagtatg
      421 aagaagcgtt gcacgacggc gtagagttcc gtttcctgaa taatccggaa cgtttcgatg
      481 ctgatggcac cttaaccttg cgcgttatgt cgcttggcga accggatgag aaaggtcgtc
      541 gccgtccggt tgaaaccaac gaaacagtaa cactgcatgt agacagcctg atcaccgcca
      601 ttggtgaaca gcaggatact gaagccctga atgcgatggg cgtgccgctg gacaaaaacg
      661 gctggccaga cgtcgaccat aatggcgaaa ctcgtctgac tgacgtcttt atgatcggcg
      721 acgtacagcg cggaccatcc tccattgtcg ctgctgtcgg aaccgcgcgt cgggcgaccg
      781 atgccatcct tagtcgggaa aatatccgtt cccaccagaa cgataaatac tggaacaacg
      841 tcaatccagc ggaaatctat caacgtaaag gcgatatctc tatcacgctg gtgaacagtg
      901 acgatcgtga cgcgtttgtc gcccaggaag ccgctcgctg cctcgaatgt aactacgttt
      961 gcaacaagtg tgtggatgtc tgcccgaacc gcgccaacgt ctccattgca gtccctggct
     1021 tccagaatag attccagacg ctgcacctcg acgcttactg taacgaatgc ggcaactgcg
     1081 ctcagttctg tccgtggaac ggtaaaccat acgaagacaa aatcaccgtc ttcagcctgg
     1141 cgcaagactt tgataacagc agcaccccag gcttccttgt ggaagattgc cgggtacgag
     1201 tacgtctgaa taaccaaagc tgagtgttaa acatcgacag cgaaggtcag tttaacaacg
     1261 taccaccgga gctgaacgat atgtgccgca tcatcagcca tgtccaccag catcatcatt
     1321 atctgctggg ccgcgtggag gtgtaatcat gttgattctg aagaatgtca ccgcagtgca
     1381 gttacacccg gcgaaagtgc aggaaggcgt tgatatcgct atcgaaaatg acgtgattgt
     1441 cgctatcggc gatgccctga cgcaacgcta ccccgatgcc agctacaaag agatgcatgg
     1501 tcggattgtg atgccgggaa ttgtctgctc gcacaaccat ttttactcgg agctttcccg
     1561 cggaattatg gcaaacatcg ccccttgccc ggatttcatc tcaacgctga aaaatctctg
     1621 gtggcggctc gatcgcgccc ttgatgaaga gtcgctctat tacagcggac tgatttgttc
     1681 cctggaagcg attaagagcg gatgtacatc ggttatcgat caccatgcct cgccggcgta
     1741 tatcggcggg tcgctctcca cattgcgcga cgcattttta aaagttggcc tgcgcgcgat
     1801 gacctgtttt gaaactactg accgtaacaa cggcatcaaa gagttgcagg aaggtgtaga
     1861 agaaaacatc cgtttcgccc gtttgattga tgaggcgaag aaagcgacaa gcgagccgta
     1921 tctggtggaa gcacatatcg gtgctcacgc gccgtttacc gtgccggatg ccggtctgga
     1981 gatgctgcgt gaagccgtga aagccacagg ccgtggtttg catattcacg ctgcggaaga
     2041 cctttacgac gtttcctaca gtcaccactg gtacggcaaa gacctgctgg cacgactggc
     2101 gcaattcgac ctcatcgaca gcaaaacgct ggtcgctcat gggctgtact tgtcgaaaga
     2161 tgacatcgcc ctactcaatc agcgcgatgc gttcctggtg cataacgccc gttcaaacat
     2221 gaacaaccat gtcggctaca accatcacct tagcgacatc cgcaacctgg cattgggaac
     2281 ggacggcatt ggttcggaca tgtttgaaga gatgaaattt gccttcttta aacatcgcga
     2341 tgcgggtggt ccgctgtggc ctgacagttt tgccaaagcc ctgactaacg gtaacgaact
     2401 gatgagccgc aactttggcg cgaaatttgg gcttctggaa gccggttaca aagctgattt
     2461 gaccatttgc gattacaact cgccgacgcc gctgctggca gacaatatcg ccgggcatat
     2521 cgctttcggt atgggctcag gcagcgttca cagcgtgatg gtcaatggtg tgatggtcta
     2581 tgaagaccgt cagtttaact tcgattgcga ttccatttat gcacaagcca gaaaagccgc
     2641 tgccagtatg tggcgtcgga tggatgcgct ggcataaatg acagatgccc tcttcccgca
     2701 gggaagaggg ttaaataagg aaagaagatg attgaacaat ttttcaggcc cgactctgtc
     2761 gaacaggcgc tggaactgaa gcgccgctac caggatgaag ccgtctggtt cgccgggggc
     2821 agcaaactca atgctacacc aacccgtacc gataaaaaga ttgccatttc cttacaggat
     2881 ctggaactgg actgggttga ctgggataac ggtgcactgc ggattggtgc aatgtctcgc
     2941 ttgcagccac tgcgtgatgc gcgctttatt cctgcagcac tgcgtgaagc cctcggtttt
     3001 gtttactcac gccatgttcg taatcagtcg accattggtg gtgaaatcgc cgcccggcag
     3061 gaagagtcag tgctgcttcc cgtcctgcta gcactggatg ctgaactggt ttttggcaac
     3121 ggcgaaacgc tgtcaatcga ggactacctg gcctgcccat gcgatcgcct gctaaccgaa
     3181 attatcatta aagatccgta tcgcacctgt gcgacccgca aaattagccg ttctcaggca
     3241 ggtttaaccg tcgtgacggc agccgttgca atgacagacc acgacggtat gcgaattgcg
     3301 ctggatggcg tcgccagtaa agcactgcgt ctgcacgatg tcgaaaaaca aaatctggaa
     3361 ggcaatgcac ttgaacaggc tgtcgccaac gccattttcc cgcaggaaga tttgcggggc
     3421 agcgtggcct ataaacgcta tatcacggga gttctggtag ccgacctgta tgccgactgc
     3481 caacaggctg gggaggaagc cgtatgatca tccactttac tttaaatggc gcgcctcagg
     3541 agctaaccgt taatccaggc gaaaacgtgc aaaagctgtt gtttaacatg ggaatgcact
     3601 ctgtacgcaa cagtgatgat ggcttcgggt ttgccggttc tgacgcaata atctttaacg
     3661 gtaatatcgt taacgcgtcc ttgcttattg ccgcacagtt agagaaggca gatattcgta
     3721 ccgcagaatc tctgggtaaa tggaacgagt taagtctggt tcaacaggca atggttgatg
     3781 ttggcgtggt gcagtctggt tataacgatc cagctgcagc tctgattatc accgatcttc
     3841 tcgatcgcat cgccgcacct acccgcgaag agatcgacga cgcgctttct ggtttgttca
     3901 gccgcgatgc aggctggcaa caatactatc aggtcattga actggcggtt gcacgtaaaa
     3961 ataatccgca ggccaccatt gatatcgctc cgactttccg tgacgacctt gacgtcattg
     4021 gcaagcatta tcctaaaact gatgccgcga aaatggtgca ggcgaaaccc tgctatgttg
     4081 aagactgcgt aacggctgac gcctgcgtca ttaaaatgtt acgtagccca cacgctcacg
     4141 cactgattac tcatctggat gtcagcaaag ctgaagcctt accgggcgtc gttcacgtta
     4201 ttactcacct gaattgccct gatatctact ataccccggg gggtcagagc gcaccggaac
     4261 cgtcaccgct tgaccgccgt atgttcggca agaaaatgcg tcacgtcggc gatcgtgttg
     4321 ctgcggtcgt cgcagaaagt gaagaaattg cgctcgaagc actgaagctc atcgacgttg
     4381 aatatgaagt gcttaagccg gtaatgtcga tcgacgaagc aatggcggaa gatgcgcctg
     4441 tcgtgcacga tgaaccggtg gtgtatgttg ctggtgcgcc agatactctg gaagacgata
     4501 acagccatgc agcccagcgc ggcgagcata tgatcatcaa cttcccgatc ggttctcgcc
     4561 ctcgcaaaaa tatcgccgcc agtattcatg gtcatattgg cgatatggac aaaggctttg
     4621 ccgatgccga tgtgatcatt gagcgaacct ataactcaac gcaggcacag cagtgcccga
     4681 ctgaaacaca tatctgcttt acccggatgg acggcgatcg tctggttatc cacgcctcca
     4741 cccaggtacc atggcactta cgccgccagg tcgcgcgcct cgtgggcatg aaacagcata
     4801 aagttcatgt cattaaagag cgagttggcg gcggttttgg ttccaaacag gacatcctgc
     4861 tggaagaagt gtgcgcctgg gcaacctgcg tgaccgggcg tccggtacta ttccgctaca
     4921 cccgtgaaga agagtttatt gctaacacct ctcgtcacgt cgcgaaagtc accgtcaaac
     4981 tgggagcgaa aaaagatggt cgcctgacgg cagtgaagat ggatttccgc gccaacactg
     5041 gcccttacgg caaccactca ctcaccgtac cgtgtaacgg accggcgctg tcgctgccgt
     5101 tatatccgtg cgataacgtc gatttccagg tcaccaccta ctacagcaac atttgcccaa
     5161 atggtgctta tcagggttat ggcgcaccga aaggtaactt cgctatcacc atggcattag
     5221 cggaactggc tgaacagtta cagatcgacc aactggaaat tatcgaacgt aaccgggtac
     5281 acgaagggca agagctgaaa attctcggtg caatcggtga aggtaaagcg ccgacctccg
     5341 ttccttccgc cgccagctgt gcactggaag agatcctgcg tcagggtcgc gagatgatcc
     5401 aatggtcgtc accgaaaccg caaaatggtg actggcacat cggtcgcggc gtcgccatta
     5461 tcatgcagaa atcgggtatc ccggatatcg atcaggctaa ctgcatgatc aaactggaat
     5521 cggacggcac ctttatcgtt cattctggcg gtgcggatat tggtactggt ctggatactg
     5581 tagtgacgaa actggcagca gaagtgctgc actgcccacc gcaggacgtg catgttatct
     5641 ccggtgatac cgatcatgcg ttgtttgata aaggcgcata tgcctcgtcc ggtacttgct
     5701 tctcgggtaa cgcggcgcgt ttggcagcgg aaaatctacg ggagaaaatt ctgttccacg
     5761 gcgcgcaaat gttgggtgag gcagtggcag atgttcaact ggcaacgccg ggcgtcgtgc
     5821 gcggcaagaa aggcgaagtt agtttcgggg atattgccca taaaggcgaa accggcatcg
     5881 gctttggttc actggtggga actggcagtt atatcacgcc tgatttcgcc ttcccgtatg
     5941 gcgcaaactt cgctgaagtt gccgtcaaca cgcgtacggg tgaaatccgc ctggataaat
     6001 tctacgcctt gctggactgc ggtacaccgg tcaacccgga attagcattg ggacaaatct
     6061 acggtgctac cctgcgagct atcggccaca gtatgagcga agagatcatt tatgacgccg
     6121 aaggtcaccc gttaacgcgt gatttacgca gttacggcgc accgaaaatt ggtgacattc
     6181 cgcgtgattt ccgcgctgtg ctggtgccga gcgacgataa agtcggcccg ttcggggcga
     6241 aatcgatctc ggaaatcggt gtaaatggcg cagctccggc gattgctacc gcaattcacg
     6301 atacatgcgg catctgggca gaagaaagtc aaagtgggca tgggaacgga tatcggtgcc
     6361 ggaacaactt tcaacatgct gcaaacgctg aacgaagcct acaaagtgtt gcaattacaa
     6421 ggctatcgcc tctcggcata tgaagcgttt tacctggcca cgctcggcgg agcgaaatct
     6481 ctgggccttg acgatttgat tggcaacttt ttacctggca aagaggctga tttcgtggtg
     6541 atggaaccca ccgccactcc gctacagcag ctgcgctatg acaactctgt ttctttagtc
     6601 gacaaattgt tcgtgatgat gacgttgggc gatgaccgtt cgatctaccg cacctacgtt
     6661 gatggtcgtc tggtgtacga acgcaactaa taataaaact ttaacatcct cgtgaggaca
     6721 tcattatgtc tggagacatc ctacaaacac cggacgcacc aaagccacag ggcgcgctgg
     6781 ataattattt taaaattacc gctcgtggca gtaccgttcg tcaggaagta ctggctggct
     6841 taacgacctt tctggccatg gtttattccg ttatcgtcgt tccgggaatg ctgggcaaag
     6901 cagtttttcc tcccgcagct gtgtttgttg ccacctgtct ggtcgcgggc ttcggctcgt
     6961 tgctgatggg gttgtaggcc aatttgccaa tggcgattgg ttgtgcgatt tccttgacgg
     7021 catttaccgc attcagtctg gtactcgggc aacaaattag cgttcctgtc gcactggtcg
     7081 cattttacgc gggtggttgg gcaatctaat agattctccg cgcctttcag cgcggagatt
     7141 tctttcacag aggattctca ccgctggcaa caatacgctg ctggcggact cgcctgagtt
     7201 ctctttcagt aagcaactgc agcgcctgtg tcgggcaaga ttcaacacag gctggcccct
     7261 cttcccgctg ttcacagaga tcgcatttca caatttgttg tcgggtatcc cccggcaacg
     7321 actgaatggt gatcattcca aacgggcacg cactgacgca actctgacag ccaatacaac
     7381 gggcagaatt ggtctgcacc acctgctcgc ccatcgtcag cgcccccacg gggcaagcgc
     7441 caacacaagg ggcgttttca cactgatggc acatcaccgg cgcactgatg ctgtccagtc
     7501 gctgtacctt cagccggggt aaaaagacat cggcattcaa ctcctgttct gaagggtgag
     7561 cgaccacaca ggctacttca caagtacggc agccaataca gtcagccgga ttaacgataa
     7621 ttaacgattt catcacgatg ccttcgtatc aaacagagtt aacatatcgc gcgccgcctg
     7681 ccttcctgcg gccattgcag tgacaaccag atccgcgcca tgaactgcat caccaccagc
     7741 aaagactttt ttcagatgcg tctgggtagg taaatacccg acgtcaccgg tttgaatcag
     7801 gccccattta tcgagtttaa ttccgctgcc ctgcaaccac ggcatggcat gcgcctggaa
     7861 accaaaggcc ataatcagaa catcggcggg caattcaaac tctgaacccg ctaccggacg
     7921 aggacgacgg cgaccatccg gccccggctc acccatggcg gtacgaatca ggcccaccgc
     7981 agttaaacgc ccatcttcgt cacaagcgat atattgcggt tgaacattga actgaaactc
     8041 gacaccttcc tcgcgcgcat taaccacctc tttgcgcgag cccggcatac tgacttcatc
     8101 acgacgatac gcgcaggtca cgctggcagc attgaggcga atggaagtcc gcaaacaatc
     8161 cattgtcgta tcgccaccgc ccaataccac gactcgctta ccttccacgt ccgtcagcgg
     8221 atactcttca gactccggca atcccatgag ctggcgggta tgggcagtca ggaacggtag
     8281 agcctgaatg acaccgggcg catcttcatg cggcagatct gctcgcatca tcccgtaagt
     8341 ccccacgccg atgaaaactg catcatattc agaagttaaa tcgctaaagg taatatcgcg
     8401 gccaatttca cagttaagat gaaagtcgat tcccattgcg gtgaatatct ctcgccgctg
     8461 gcttaatacc gttttatcga gtttgaaagg aggaatgcca aaagtcagca taccgccaat
     8521 ttctggatgg cgatcaaaga catcaacctg aactcccgcg cgcgccagaa tatcagcaca
     8581 ccctaaccct gctggcccag cgccaatcac cgccactttt tcgatacggg gaacaacttt
     8641 gctgacatcg ggacgccagc ccatcgccag cgcggtatcg gtgatgtagc gttccagatt
     8701 accgatagag actgcgccag agtgatcttt caaagtacat gcgccttcac aaagacggtc
     8761 ctgtgggcat accctgccgc agatttctgg taaggaactg gtctggtggc aaagttctgc
     8821 cgcttcaata atctttcctt cctgtaccag acggatgtaa tccggaatag cgttatgcag
     8881 cgggcaatgc cagttgcagt tagctttttc ggcacaataa acacagcggt cactctcata
     8941 agtcgcttgc agtggatcca gcccgcaata gatttcgcca aagtgggttt tccgttcact
     9001 cgctgaaatt ttatctgcgc ctttacgcga gttaacgggg agcaacgctg cactgcgaga
     9061 tggctgagcg tctgatgacg cttttcctgc tgccgttttg cgctggcgag ccacctttat
     9121 ccactgtaac cctttatcgt ccatcagtcg taacgcctgt gttgggcaga cttcaataca
     9181 ggcttgcgtg ccggaactgc gctggttaca aaggtcgcat ttctgcgcaa tcgtatcgac
     9241 catctcaacg acgccaaagg ggcaagcgat tgcgcatctt ttacagccaa tacatttttg
     9301 ctcgtccagt tgtacgctat cggactggaa agtcagagca ttaaccggac aagccgtaac
     9361 acaaggggca ttgttgcagt gatggcaggc caccggattc gcagcctggc ctttccctac
     9421 aacgtggata cgcggtcgaa agtcactgtg actcagcggc cagttttctt gattgtgtgc
     9481 caccgcacag gcaatttcac aagcatgaca gcctatacat tccgcagctt cagcagcgat
     9541 aaacttattc atttgcatcc ctttcat
//
LOCUS       DAJTVJ010000151         9504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000151 DAJTVJ010000000
VERSION     DAJTVJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.151"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(149..1231)
                     /locus_tag="OO448_003674"
     CDS             complement(149..1231)
                     /locus_tag="OO448_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCR6338785.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="OO448_003675"
     CDS             complement(1228..3024)
                     /locus_tag="OO448_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6338786.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="OO448_003676"
     CDS             complement(3275..3631)
                     /locus_tag="OO448_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338787.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="OO448_003677"
     CDS             complement(3689..4111)
                     /locus_tag="OO448_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6338788.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="OO448_003678"
     CDS             complement(4127..4852)
                     /locus_tag="OO448_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR6338789.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="OO448_003679"
     CDS             complement(4874..5620)
                     /locus_tag="OO448_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6338790.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="OO448_003680"
     CDS             complement(5627..6691)
                     /locus_tag="OO448_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR6338791.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="OO448_003681"
     CDS             complement(6763..7188)
                     /locus_tag="OO448_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6338792.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="OO448_003682"
     CDS             complement(7185..7448)
                     /locus_tag="OO448_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6338793.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="OO448_003683"
     CDS             7560..8060
                     /locus_tag="OO448_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6338794.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="OO448_003684"
     CDS             8080..8274
                     /locus_tag="OO448_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6338795.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="OO448_003685"
     CDS             8274..8564
                     /locus_tag="OO448_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6338796.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8845..8997
                     /locus_tag="OO448_003686"
     CDS             8845..8997
                     /locus_tag="OO448_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6338797.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8997..9374
                     /locus_tag="OO448_003687"
     CDS             8997..9374
                     /locus_tag="OO448_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338798.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2445 a         2493 c         2017 g         2549 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaaa taatgatgct attgtttcag gcgtcaaccc accccgcccc acagaatgca
     8701 gggcaatact tcgagttacc agacagtggt caggggttaa gtagccagcc cgaggcgtaa
     8761 gaacatgacg gcagggttca atctttaact atgcagcagg ttttttgttc cgccaccccg
     8821 gcgttaaggg gaaatgaggt cagcatggat actatcgatc ttggcaacaa cgaatctctg
     8881 gtgtgcggcg tgttccctaa ccaggatgga acgtttaccg ccatgacgta taccaaaagc
     8941 aaaacgttta aaaccgaatc tggcgcgcgt tgctggttag ccagaaacac tgactgatga
     9001 ggctgacgat ggaatttaaa gatttaccgt ctgatgtaca gaaaacagca gctcatacat
     9061 tgcattctgt gctgcgagaa atcgggaaag atattgcaag cgagccagca aaagatctgg
     9121 cccggaaaat caagaccgct ttcgttgagc tttataatgt tggcactgac tctgaaactg
     9181 tcgagaccaa gaccgtaagt tcaccaatat tctcacttga gccaagcgta ttatcaggtg
     9241 aaatatgcac cgagatttcc agcgaactgc ttccggtaat acgagaagca attcgccgtc
     9301 gcggtttgga tggaagttat gatcatgacg ttctgcaagt tctcaggaca atggtgactt
     9361 cactggggat ttgatccctg cgtcctctaa gcttttgata tagcggtcct ccaagaagcg
     9421 gtaaattttg tcgaaatcat cttttccctc tggtttattc agaggattac aggacccgta
     9481 agtagcggca tacatttcaa ccgt
//
LOCUS       DAJTVJ010000152         9433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000152 DAJTVJ010000000
VERSION     DAJTVJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.152"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="OO448_003688"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="OO448_003688"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6338799.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="OO448_003689"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="OO448_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6338800.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="OO448_003690"
                     /pseudo
     CDS             1394..1915
                     /locus_tag="OO448_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="OO448_003691"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="OO448_003691"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6338801.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="OO448_003692"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="OO448_003692"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6338802.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="OO448_003693"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="OO448_003693"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6338803.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="OO448_003694"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="OO448_003694"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6338804.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="OO448_003695"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="OO448_003695"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6338805.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="OO448_003696"
     CDS             complement(6557..7450)
                     /locus_tag="OO448_003696"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6338806.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="OO448_003697"
     CDS             complement(7447..8100)
                     /locus_tag="OO448_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6338807.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="OO448_003698"
     CDS             complement(8111..9370)
                     /locus_tag="OO448_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6338808.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2160 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gcctgaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccaatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaacccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cctgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa acgagccagg taatgactcc
     9421 aacttattga tag
//
LOCUS       DAJTVJ010000153         9420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000153 DAJTVJ010000000
VERSION     DAJTVJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.153"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            223..2199
                     /locus_tag="OO448_003699"
                     /pseudo
     CDS             223..2199
                     /locus_tag="OO448_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3328
                     /locus_tag="OO448_003700"
     CDS             2192..3328
                     /locus_tag="OO448_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6338809.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="OO448_003701"
     CDS             3325..5325
                     /locus_tag="OO448_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6338810.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="OO448_003702"
     CDS             5447..5908
                     /locus_tag="OO448_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6338811.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="OO448_003703"
     CDS             complement(5949..6419)
                     /locus_tag="OO448_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6338812.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO448_003704"
     CDS             complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO448_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6338813.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO448_003705"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO448_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6338814.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="OO448_003706"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="OO448_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT         2291 a         2491 c         2416 g         2222 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
//
LOCUS       DAJTVJ010000154         9397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000154 DAJTVJ010000000
VERSION     DAJTVJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.154"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(350..1603)
                     /locus_tag="OO448_003707"
     CDS             complement(350..1603)
                     /locus_tag="OO448_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6338815.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="OO448_003708"
     CDS             1710..2603
                     /locus_tag="OO448_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6338816.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="OO448_003709"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="OO448_003709"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6338817.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="OO448_003710"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="OO448_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6338818.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO448_003711"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO448_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6338819.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALSASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO448_003712"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO448_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6338820.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="OO448_003713"
     CDS             complement(6839..7693)
                     /locus_tag="OO448_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6338821.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO448_003714"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO448_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6338822.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="OO448_003715"
     CDS             complement(8323..>9397)
                     /locus_tag="OO448_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6338823.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2378 c         2439 g         2131 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacaggtaca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcgga aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAJTVJ010000155         9220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000155 DAJTVJ010000000
VERSION     DAJTVJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.155"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            74..733
                     /locus_tag="OO448_003716"
     CDS             74..733
                     /locus_tag="OO448_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6338824.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            837..1811
                     /gene="ghrB"
                     /locus_tag="OO448_003717"
     CDS             837..1811
                     /gene="ghrB"
                     /locus_tag="OO448_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6338825.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1861..2571)
                     /locus_tag="OO448_003718"
     CDS             complement(1861..2571)
                     /locus_tag="OO448_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6338826.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3005..3295
                     /locus_tag="OO448_003719"
     CDS             3005..3295
                     /locus_tag="OO448_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6338827.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3576..3788
                     /gene="cspA"
                     /locus_tag="OO448_003720"
     CDS             3576..3788
                     /gene="cspA"
                     /locus_tag="OO448_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6338828.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3976..4128)
                     /gene="hokA"
                     /locus_tag="OO448_003721"
     CDS             complement(3976..4128)
                     /gene="hokA"
                     /locus_tag="OO448_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6338829.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4454..6523)
                     /gene="glyS"
                     /locus_tag="OO448_003722"
     CDS             complement(4454..6523)
                     /gene="glyS"
                     /locus_tag="OO448_003722"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6338830.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6533..7444)
                     /gene="glyQ"
                     /locus_tag="OO448_003723"
     CDS             complement(6533..7444)
                     /gene="glyQ"
                     /locus_tag="OO448_003723"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6338831.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7539..7838)
                     /locus_tag="OO448_003724"
     CDS             complement(7539..7838)
                     /locus_tag="OO448_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6338832.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8013..9008
                     /gene="wecH"
                     /locus_tag="OO448_003725"
     CDS             8013..9008
                     /gene="wecH"
                     /locus_tag="OO448_003725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6338833.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9050..>9220)
                     /locus_tag="OO448_003726"
     CDS             complement(9050..>9220)
                     /locus_tag="OO448_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338834.1"
                     /translation="LMVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPP
                     KETQVTQEEYSE"
BASE COUNT         2288 a         2331 c         2269 g         2332 t
ORIGIN      
        1 ttcatgttca cgcggcataa tctcccgcca cggaacccgt ggcaaagaat aaaaaggtta
       61 ttaaggatta acaatgaaga aacgtgttta tcttattgcc gccgtagtga gtggcgctct
      121 ggcggtatct ggctgcacaa ctaaccctta caccggcgaa cgcgaagcag gcaaatctgc
      181 tatcggcgca ggtctgggct ctctcgtggg cgcgggtatt ggtgcgctct cttcttcgaa
      241 gaaagatcgc ggtaaaggcg cgctgattgg cgcagcagca ggcgcagctc tgggcggcgg
      301 cgtgggttat tacatggatg tgcaggaagc gaagctgcgt gacaaaatgc gcggcactgg
      361 tgttagcgta acccgcagcg gggataacat tatcctcaat atgccgaaca atgtgacctt
      421 cgacagcagc agcgcgaccc tgaaaccggc aggcgctaac accctgaccg gcgtggcaat
      481 ggtgctgaca gagtatccga aaacggcggt taacgtgatt ggttataccg acagcacggg
      541 gggtcacgac ctgaacatgc gtctctccca gcagcgtgcg gattccgttg ccagcgcgtt
      601 gatcacccag ggcgtggacg ccagccgcat ccgtactcag ggccttggcc cggctaaccc
      661 aatcgccagc aacagcaccg cagaaggtaa ggcgcaaaac cgccgtgtag aaattacctt
      721 aagcccgctg taatcccttt catgccaggt gatgcagaaa tcacctggca ttcttcagtt
      781 ttccttcatc atatttcagg ctaaggtgat cgccttatca gtgaatggag agaagcatga
      841 agccgtccgt tatcctctac aaagccttac ctgatgattt actgcaacgc ctgcaagagc
      901 atttcaccgt tcaccaggtg gcaaacctca gcccacaaac cgtcgaacaa aatgcagcaa
      961 tttttgccga agctgaaggt ttactgggtt caaacgagaa tgttgatgcc gcattgctgg
     1021 aaaaaatgcc gaaactgcat gccacatcaa cgatctccgt cggctatgac aattttgatg
     1081 tcgatgcgct taccgcccga aaaattctgc tgatgcacac gccaaccgta ttaacagaaa
     1141 ccgtcgccga tacgctgatg gcgctggtgt tgtctaccgc tcgtcgggtt gtggaggtag
     1201 cagaacgggt aaaagcaggc gaatggaccg cgagcatagg cccggactgg tacggcactg
     1261 acgttcacca taaaacactg ggcattgtcg ggatgggacg gatcggtatg gcgctggcac
     1321 aacgtgcgca ctttggcttc aacatgccca tcctctataa cgcgcgccgc caccataaag
     1381 aagcagaaga acgcttcaac gcccgctact gcgatttgga tacactgtta caagagtcag
     1441 atttcgtttg cctgatcctg ccgttaactg atgagacgca tcatctgttt ggcgcagaac
     1501 aattcgccaa aatgaaatcc tccgccattt tcattaatgc cggacgtggc ccggtggttg
     1561 acgaaaatgc actgatcgca gcattacaga aaggggaaat tcacgccgcc gggcttgatg
     1621 tcttcgaaca agagccactg tccgtagatt cgccgttgct ctcaatggcc aacgtcgtcg
     1681 cagtaccgca tattggatct gccacccatg agacgcgtta tggcatggcc gcctgtgccg
     1741 tggataattt aattgatgcg ttacaaggaa aggttgagaa gaactgtgtg aatcctcacg
     1801 tcgcggacta agccgcgact gcgtgaagta aagcccgata atcgctcggg cttttactct
     1861 ttattgggtt gcagtaactg ctgtagtcca ggcctgatta aacgcctgat gttgtgccgg
     1921 taatggcgca atcagtttgt tatattcact tgcctgctgt gaagtcggga actggatgcc
     1981 atttgcgaca aagctcacct gagtaccttg ctgagcaatg tagtctccaa ccatcaccag
     2041 ttgttgcgtg aaagtttgtg ccgccggaat caacggttgc aatgcatcag ctggcgtcgt
     2101 taccactttg gtgaatgcct gatcaaatac cggtttcagg tcatcgctct gttttagcgc
     2161 agagtgcgca gcatcggcct gcaacttcgc attttgcagt tgttgcgcca gcacacccag
     2221 cgaaccattc atttcacgca gcggaccgct ttgcgtaacg taatcctgcg gtacgcgaat
     2281 tgcgttaacg ctgtctacga ccgggcgcag accagaatcc atcgcctgat tcacctgctg
     2341 agaataacca tacagaatcg cgtaatcaga gacaaaagga ccaaactgtt ttttctgatc
     2401 ggcagtcagg gttggtagac gttcaccgct acgcatcact gtattctgca ggaagtcgat
     2461 aaacgcttta cgctgatcgc cttctttatc aaaacaccca ctcaggctaa ctaccattaa
     2521 taacgccgca agaggcgcaa accagcgaga gcaggacttt cctgtcgcca ttttcttact
     2581 cctttcaccc aaaaaaagcg cacaacgaca cacgcgtgtc tgacgtgaca aggatagtcc
     2641 aggacaggct tgcaggatac cctttctacg taatcttcct ggtaaaatca tggagaaaat
     2701 gaaacaaggt taatttgttg tttcattgtt aaaaaatgat agtgccgttg ccttttttgt
     2761 agccagagat ttacgccttc gcagaaggtt agattttcga aatctttaca aaacaggagt
     2821 ggtaagagat atatcggcat ttatcagatc gttaggtggc tataagtcac gctaaatgac
     2881 aggctgaatc gaatcatagc cagagcatgc cctgacttca ccccgctgtg tctgcttttc
     2941 ccgactattc ttaatgagct tcgatgcaat tcacgatccc gcagtgtgat ttgaggagtt
     3001 ttcaatggaa tataaagatc caatgcatga gctgttgagc agcctggaac agattgtttt
     3061 taaagatgaa acgcagaaaa ttaccctgac gcacagaaca acgtcctgta ccgaaattga
     3121 gcagttacga aaagggacag ggttaaaaat cgatgatttc gcccgggttt tgggcgtatc
     3181 agtcgccatg gtaaaggaat gggaatccag acgcgtgaag ccttcaagtg ccgaactaaa
     3241 attgatgcgt ttgattcaag ccaacccggc attaagtaag cagttgatgg aatagacttt
     3301 tatccacttt attgctgttt acggtcctga tgacaggacc gttttccaac cgattaatca
     3361 taaatatgaa aaataattgt tgcatcaccc gccaatgcgt ggcttaatgc acatcaacgg
     3421 tttgacgtac agaccattaa agcagtgtag taaggcaagt cccttcaaga gttatcgttg
     3481 atacccctcg tagtgcacat tcctttaacg cttcaaaatc tgtaaagcac gccatatcgc
     3541 cgaaaggcac acttaattat taaaggtaat acactatgtc cggtaaaatg actggtatcg
     3601 taaaatggtt caacgctgac aaaggcttcg gcttcatcac tcctgacgat ggctctaaag
     3661 atgtgttcgt acacttctct gctatccaga acgatggtta caaatctctg gacgaaggtc
     3721 agaaagtgtc cttcaccatc gaaagcggcg ctaaaggccc ggcagctggt aacgtaacca
     3781 gcctgtaatc tctgcttaaa agcacagaat ctaagatccc tgccatttgg cggggatttt
     3841 tttatttgtt tttcaggaaa taaataatcg atcgcgtaat aaaatttagt attatttttg
     3901 tgaagaataa atttgggtgc aatgagaatg cgcaacgccg taagaaaggc gggaataact
     3961 tcccgccaaa gactcttact ctttcaaatt gcaggctaaa aatgccgcca gctcataact
     4021 ctcctgttta atatgcaatt cacacagtga atctcttatc atccaggtga aaaataaaag
     4081 cgtgaaacaa atcactatta aagaaagtaa tctatatttc tgcggcattc ccagcctcct
     4141 gtgttgattt ccaacgagta agaggctaac cttatggtgt tcaggcatag ggtagcctca
     4201 ctttgattta tagtcaggtg gggcttttct ctgtctgcct ttcggtgaat acctgagaca
     4261 aacagtctca agcacccgtg gctattctag cttaataagt ttgtttcttc tccttgatat
     4321 aatcctaaaa aaacctcata aaattaatat atgagataat ctttattcaa cagaagatta
     4381 ttaaaggttg ctgtattatt tagcgataat aaaaaaagcc tgccagatgg caggcttttt
     4441 aataacggcg ttattactgc aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca
     4501 gtttctccag catggtcaga cggttgagac gcaattcttt gtcatcaacc atgaccatca
     4561 ctttatcgaa gaaagcatca accggctcac gcagctctgc cagttcgacc agcgcatcct
     4621 ggtaacgacc ttccgcaaag tacggctcca gcttgtcacg tagcaccaca acctgcatcg
     4681 ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcggtcg ctcagcactt
     4741 cgtcagattt cgccagaatg ttagatacac gtttgttcgc cgccgccagt gcagcagctg
     4801 catccagggt acggaaatgc gataccgctt tcatacgggc atcgaaatca gccggacgag
     4861 tcggacgacg cgccagtacc gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc
     4921 aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aactacgttg gcgttagtca
     4981 gcttatcgcc atacagacgc actgcttctt cggtcagcgt ttgcagatca aggttgaggt
     5041 tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca aacgggtctt
     5101 tgtcgccttt cggatgctga ccgataccga agatacccgc cagagtatcc atcttatcag
     5161 caatcgccag cgcacaagct accgggttgg acggcaggtc atcaccggca aagcgcggct
     5221 gatactgctc attcagcgcg acggctacgt cttccgcttc gccatcgtga cgcgcgtagt
     5281 gcatccccat aacgccttgg gtgtcggtga actcgaagac catgttggtc atcaggtcac
     5341 acttagacag caggcccgca cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa
     5401 tccagccagc cagcgcctgg atgcggtcag ttttgtcacg cagcgtaccc agctgttgct
     5461 ggaacagcac ggtttgcagg cgcggcaggt tatcttccag acgtttttta cggtcggtat
     5521 tgaagaagaa ctcggcatcc gccagacgtg gacgaacgac tttctcgtta ccagagataa
     5581 tttgctgcgg atctttcgat tcgatgttgg caacgaagat gaagttcggc agcagtttgc
     5641 catcgttcgc atacaccggg aagtatttct ggtcaccttt catggtgtaa accagggctt
     5701 cagccggcac cgcgaggaat ttctcttcga attttgcggt cagaacgacc ggccactcca
     5761 ccagcgaagc cacttcttcc agcaggcttt cgcttaagtc agcgttaccg ccaatcttac
     5821 gcgctgcttc ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa tcggcgatga
     5881 ctttcccgcg ctcacgcaga atttccggat actgatcggc gttgtcgatg gtgaattccg
     5941 gttcgcccat aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg
     6001 ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc acgaagtgca
     6061 cgtcgcttgc gccccaacgc atcagtttcg ggatcggcag tttcgccagc gaagtcgcaa
     6121 ccatattcgg cagtagtgct tcagtgcttt cgcccttcac atgggcgcga tacagcagcc
     6181 attcgccttt atcggtagtc agacgctcag cctggtcaac ggtaataccg caaccacgcg
     6241 cccaaccttc tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg
     6301 ggccgcgttt ttcgatttca cgatctggtt gcgcttccgc caggttagct actttcaacg
     6361 ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca
     6421 gctccgcagt aaagttcgca gcaaaggact cagccaggct gcgcagtgct tttggtggca
     6481 gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc tcttacttat
     6541 ctttgttgca catcgggaag ccgagggctt cacgggaagc gtagtatgct tctgccactg
     6601 ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcagtgacg gagatggctt
     6661 tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg taggctggca
     6721 gcggcagcgg attttccagc gccagcagct gctgcgcttc tttctcgtac tgctcgaagc
     6781 aggtgaacag gaaatccaca tccgcgtatt cgaagttgta agtggactgc tccacttcgt
     6841 tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc cagaccaggt
     6901 cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg taggtgatct
     6961 cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg aactgcgtca
     7021 cttccatgcc gtttagccac acttcccagc ccagtcccca ggcacccaga gtcgggtttt
     7081 cccagttatc ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc agctctttca
     7141 gagaaccgag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc acctggaact
     7201 gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc gggcgacgag
     7261 aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga caggtcattg
     7321 ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg cagccctggc
     7381 gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta tcaaactttt
     7441 gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta tacccgctgg
     7501 atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga aagatgcaaa
     7561 aaatggccat ctgcgtcaaa agaacagaca aactgctctt tacggaaggt ataaccgcgc
     7621 atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg
     7681 ttataacgtt ttgccgcctg atccttgcat aacgcttcca tattcaacga acgcagagga
     7741 tcaaccttta cccgttgtgc tttctgcacg ggtgacggtg tagaccaacc cactagcacc
     7801 ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc tggtttgcag
     7861 gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc cttaagcctc
     7921 tataagctgt agtgataaaa ctcagattat tccagtgcgc caaccaaatg atttatcggt
     7981 cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac tggattgata
     8041 acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc tggtatgtga
     8101 ccaatgctca tagtgttagc cccgtcacat gggatatcgc caatgttctg aattctgcct
     8161 ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt ggcgaacgca
     8221 gcgcccagcc gcgccatttc ttgcgtatcg gcttatgtct gattttttat agcgcaatcg
     8281 cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg aaaaacctgc
     8341 tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg atttatctgg
     8401 tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg ctaatggcgg
     8461 tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt tttgaatggc
     8521 tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat ggcatgttgg
     8581 gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg agcgccgcgc
     8641 tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg cagtggcgcg
     8701 gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc tgcgcaatcg
     8761 cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga cttggcttaa
     8821 tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat gcgctgcgca
     8881 cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc ttttgcgcga
     8941 cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga aacagattag
     9001 tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat tattcgctgt
     9061 attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggcatca cgaatattct
     9121 tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa aaaccttttt
     9181 cactgagtaa taatgtcgca ttccacagac ctaccatcag
//
LOCUS       DAJTVJ010000156         9126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000156 DAJTVJ010000000
VERSION     DAJTVJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.156"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            375..1250
                     /locus_tag="OO448_003727"
     CDS             375..1250
                     /locus_tag="OO448_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR6338835.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1258..2034
                     /locus_tag="OO448_003728"
     CDS             1258..2034
                     /locus_tag="OO448_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR6338836.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2203..4464
                     /locus_tag="OO448_003729"
     CDS             2203..4464
                     /locus_tag="OO448_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6338837.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4533..5708
                     /gene="senB"
                     /locus_tag="OO448_003730"
     CDS             4533..5708
                     /gene="senB"
                     /locus_tag="OO448_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR6338838.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6234..6695)
                     /locus_tag="OO448_003731"
                     /pseudo
     CDS             complement(6234..6695)
                     /locus_tag="OO448_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6733..8271)
                     /locus_tag="OO448_003732"
     CDS             complement(6733..8271)
                     /locus_tag="OO448_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6338839.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPMEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
     gene            complement(8321..8668)
                     /gene="tnpB"
                     /locus_tag="OO448_003733"
     CDS             complement(8321..8668)
                     /gene="tnpB"
                     /locus_tag="OO448_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6338840.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(8665..9045)
                     /locus_tag="OO448_003734"
     CDS             complement(8665..9045)
                     /locus_tag="OO448_003734"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR6338841.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
BASE COUNT         2373 a         2260 c         2215 g         2278 t
ORIGIN      
        1 ttaacgtacg ttttcgttcc attggccctc accccccgat aacgacccag gtagcgcgcc
       61 gtattccccc cgccggccgt tttcttcgac atgtacatca ggcgcggaac tcatcggggc
      121 gcgggaatga aatcccattc agactccgga tagattcaag caagcccact tatcgtcaaa
      181 atcggtgttg caaaaacggg agtgaccata gatccgtttt ccaagcggac cccataatat
      241 cggctggtaa acatcactcg ctagtgcatt ctttttgttg gtaatgataa tcatttacat
      301 tcattctttg atgttttatc ataattaatg ttttgtcaat aaatatatgg aaacatattg
      361 tttcagatgg gaatatgaga aagttcatcc ttatttctat gataacgtta gctagtgtta
      421 gtctgggagc ctgcagacag aatgtcacca taaagcagga tgcgccaggg cagaaagctt
      481 tcctgactga cgggcagatt tacgatttgc acagtggcaa aattatttct agcagcgagc
      541 ttttggcgga tcttgctacc gctcgacatc ttattattgg tgaaaaacac gataatgccg
      601 agcatcatca gattgagctt tggcttattc aaaatttgct catccagcga ccacagggga
      661 gtgtgttgct tgagatgttg acgtcagagc aacagccccg agttaaccaa gttaaatgtt
      721 ggctgaaaga taatcctgtg gttcgagata gccgtgttca ggaactactt aactggcaaa
      781 aagggtggtc ctgggagatg tatggcgaca tcgtcatgca gctattacgt gggccttacc
      841 cgctgctcaa tgccaacatt ggccgggagc agatgttggc actgtacaaa aaaaacgaat
      901 ttcctaaagg aaaaaaatcc actgcacctg tggtacaaga agctctgcgt gaaaccatca
      961 tatctatgca tgaaggcaac cttgaaagcc agcaactgac gtcaatgctc tctattcagc
     1021 agcagcggga tcgctacatg gcccgccagc ttctgagtgc cccggtaccg tcgttgttga
     1081 ttgcaggtgg ttatcacgcg tcaaaaagca tgggcgtacc gttacacatg gaagatcttg
     1141 ccaccggcac ccatccagtg gtgttgatgc ttgcagaaaa aggaatgaac atcacagtag
     1201 atcatgctga ctacgtgtgg tttgtagcgc cagatacaac aaaaaggtaa accatggatg
     1261 atgaatattc tccacttctc acagtcggta aaatggagca gctggtttat ctgttctcta
     1321 ctgcttcatg gactgatttt ccttgcgttt atctggcgtt tttccgaagt tcagcccgct
     1381 atgtctcctg caccggcgat catgctgcaa tgggcagaaa ttatagaggc tccttcttct
     1441 ccactttcgc ttccggtggg aatcgctcaa caggagtccg cagtgacaga agaaaagcaa
     1501 caaactgagg acagacaaca acgtcctgtc actgaagaca gtgatgcaac cattgaaata
     1561 actaggaaga aaaagtcatc cgatggagaa aagaaaaaga cacgtccacc tcgtaagata
     1621 aaggctcaaa ccagcgacag caaccccaca gccgtttcca gtaatgctgc acctcaggct
     1681 ctggtcgaat cgtcccgtat tgctgcgcct tttaacagtg actcaacaaa gcgagataat
     1741 tctgaggcat cttgggaaag cagagtaaaa ggccacctaa accgttataa acgttaccct
     1801 ggcgatgcca gaaaacgtgc acgaaccggc acagccgtcg tgacttttac cgtaaacacg
     1861 gaaggcacta ttgtcagtag tttcttggaa atctcttcag gaaccctttc tcttgaccgc
     1921 gaagctattg ctgttttaga acgcgctcaa ccacttccca aaccaccgcc tgaaatattg
     1981 gagggaggat tatttaaagt aaaaatgccg attaccttta agttgaagga gtaaagggat
     2041 aattggccgt tcaagctcat ttaactgaaa tttaattttc atttttatgc tgaacatttt
     2101 atcaaacttt tccactgtcg aaaaaatcaa aaagacagta gaacataatt aataaacaaa
     2161 tagttatact tatctttatt aatattatac ccaaggaaat ttatgaacgt tataaaactg
     2221 gctatcggct caggcatatt attgctcagc tgcggtgctt actcacaatc catcagtgaa
     2281 aaaactaatt ccgacaaaaa aggagcggca gaattcagtc cgctcagcgt ttctgtcggg
     2341 aagacgacca gtgagcagga agctctcgag aaaacaggcg cgaccagttc ccggacaacg
     2401 gacaaaaacc tgcaatcact tgacgcaaca gtgcgtagta tgcctggtac ttatactcaa
     2461 atagatcctg gtcagggagc aatcagtgtg aatattcgag gcatgagcgg atttggtcgt
     2521 gtaaacacta tggtcgatgg tattacccag agtttttacg gaacctctac ctccggaaca
     2581 acgacgcatg gttcaactaa caatatggct ggcgtactta tagatcctaa cttactggta
     2641 gcagttgatg ttacacgcgg tgacagcagt ggctctgaag ggatcaacgc ccttgccggt
     2701 agtgcaaata tgcgtactat tggcgttgac gatgtaatat ttaacggtaa tacatatggc
     2761 cttcgttcac gtttctctgt cggtagtaat gggctgggac gcagcggaat gatcgccctt
     2821 ggtggaaaaa gcgacgcttt tacggatacg ggaagcattg gcgttatggc tgctgtgagc
     2881 ggcagttctg tgtactctaa tttctcaaat ggttctggaa ttaacagcaa agagtttggt
     2941 tatgataaat atatgaagca gaaccccaaa tcccaactgt ataaaatgga tatcagacca
     3001 gacgaattta acagcttcga actttccgct cgaacctatg aaaataaatt tacacgtcgt
     3061 gatataacca gtgacgacta ttacattaaa tatcattaca cccctttttc tgaattaatt
     3121 gactttaacg taacggccag taccagtcgc ggtaatcaaa agtatcgtga tggctcgctg
     3181 tatactttct acaaaacctc agcgcaaaat cgttctgacg cgctggatat caacaatacc
     3241 agccggttca ctgtcgcgga caatgacctg gagtttatgc tgggcagcaa actgatgcgt
     3301 acccgctatg accggaccat tcactcagcg gcgggcgacc cgaaagcgaa tcaggaatcg
     3361 atcgagaaca atccgttcgc accctccggc cagcaggata tttcagcgct gtataccggg
     3421 ctgaaggtta cgcgcggcat ctgggaggca gatttcaatc tcaactacac acgtaacagg
     3481 atcacagggt acaagcccgc ctgcgattca cgcgttatct gcgtgccaca gggtagctac
     3541 gatattgacg ataaagaggg tggcttcaac ccttcagttc agctttctgc tcaggtaaca
     3601 ccatggcttc agccgttcat tggctacagc aaatccatgc gcgccccgaa catccaggag
     3661 atgttcttct ctaattcagg aggcgcatcc atgaacccat tcctgaagcc tgaacgtgca
     3721 gaaacctggc aggcgggttt taacattgat accagagatt tactggtcga acaggatgcc
     3781 ctgcgcttta aggctctggc gtaccgcagc aggatccaga actacatcta cagcgagtct
     3841 tatctggttt gttctggagg tcgtaaatgc agtatgcctg aggtgattgg caatgactgg
     3901 gagggcatta gcgatgaata cagcgacaat atgtacatct acgttaactc ggcaagcgac
     3961 gttatcgcaa agggcttcga actggagatg gattatgatg caggttttgc ttttggccga
     4021 ctctctttca gccagcagca aacagaccag ccaacctcca tcgccagcac ctactttggc
     4081 gcaggggata tgaccgaact gcccagaaaa tacatgacgc tggatactgg tgttcgcttc
     4141 ttcgataacg cgttgaccct gggcactatc ataaaataca caggcaaggc tcgtcgcctg
     4201 tcgcctgatt ttgagcagga cgaacatacc ggcgcaataa tcaaacagga tttgccgcag
     4261 atcccaacga ttatcgatct ctatggtact tacgagtaca accgcaacct aacactgaaa
     4321 ctttcggtac aaaacctgat gaacagagat tattcggagg cgctgaataa gctcaacatg
     4381 atgccaggtc ttggtgacga gacccaccca gccaattccg cgcgtggcag aacatggata
     4441 tttggcgggg acattcgttt ctgatgacaa tttccgctag gttcagacaa tacgttttct
     4501 ctcttatgtc aattttattg caggaacgaa aaatgaatat tttcacttta tccaaagcac
     4561 cgctatacct gttaatttca ctatttttac ccacgatggc catggctatc gatccacctg
     4621 aacgcgaact ttcgcgattt gccctgaaaa cgaattacct tcagtcccct gatgaaggcg
     4681 tctatgaact ggcgtttgat aatgccagta aaaaggtgtt tgcagcagtc accgatcgtg
     4741 taaatcgtga agccaataaa ggctatctgt attcgtttaa ttcagattcg ctgaaagtcg
     4801 aaaataaata cacgatgcca taccgggcat tttcgctggc gataaatcag gataaacatc
     4861 agctctatat cggacacacc cagtcagcgt ccctgcgtat cagtatgttt gacaccccaa
     4921 ccggcaaact ggtaagaacc agcgacaggt taagttttaa agcggcaaac gctgcagatt
     4981 cgcgttttga gcattttcgc catatggttt acagccagga ttccgatacc ctgtttgtga
     5041 gttatagcaa tatgctgaaa acggccgagg gcatgaagcc tctgcataag ctgttaatgc
     5101 tcgacgggac gacgcttgcc ttaaaaggcg aggttaagga tgcttacaaa ggtacagcgt
     5161 atggtctgac gatggatgaa aaaacacaga aaatctacgt tggcggaaga gattacatca
     5221 acgaaattga tgcgaaaaat cagacgctgc tgcgtaccat cccgttgaaa gatccgagac
     5281 cacaaatcac aagtgtgcag aatctggcgg tggactccgc ttctgaccgt gcctttgtgg
     5341 tggtattcga ccatgacgat cgttccggta caaaagatgg actctatatt tttgacttac
     5401 gcgacggtaa acagcttggc tatgtgcaca caggagccgg agctaacgcg gtgaaataca
     5461 atccgaaata taacgaactg tatgtcacca acttcactag cggcaccatc agcgtagtgg
     5521 atgccaccaa atacagcatc acccgtgaat ttaacatgcc ggtctaccca aaccagatgg
     5581 tgttgtcgga cgatatggat accctttaca ttggcatcaa agaaggcttt aaccgcgatt
     5641 gggatcctga tgtgtttgtg gaaggagcta aagaacgtat tctgagcatt gatttgaaaa
     5701 agtcgtgatc ggggggaaaa aacatgggat aaaaactccg ttattccact aaactggccg
     5761 gactaggaaa agcaaacatc tctgtcctgc cggtacgcct gttacgccgc tccatgaggc
     5821 tgaggaatct ccagaaaacg agacaggcac agcattgtac gatcaactga tttgtgccaa
     5881 taattaaatg aaatcacagc tatgcctgtt catggggttc taggccggaa ccccagattt
     5941 ttccgattgt ttgttttcta actgattatt cagtagtttt agagggctga cgctcagtgg
     6001 aacgaaaact cacgttaaga ggttttgact atgataaaag tcaaagtagg ggtgagtttg
     6061 gatatggaaa ttattgtact ataaggagag catgaaaacc cctggggcag tcactgaatg
     6121 agggaccagc agcggtaaaa taaccagcat aatttctgca tggtcgattt ttataccgtg
     6181 acaggctgtt ttttatactt ggggtcagtt tggatatcga aaattattgt acgttaaggt
     6241 tgttttttga ccttgtcgat cttaactgat ctcaaacgac gaaacttacc tgaagtcggt
     6301 atccggctgt ttttccagat ataatcgcct gtcagattga tatgttccca acctaatggt
     6361 gataaatgcg ataatagttg agcatttatt ttcatgccct tacgcttcag agcatcaacc
     6421 gctctttcca tgtaaactgt gttccataag gaaatagccg ccgtcaataa agtcaatcca
     6481 ctggcgcgat aactttgatt ttccggtttc cgatctctaa tctcccccaa tcgatgcatg
     6541 aatacagcac gggccagagc attacgggct tctcctttgt tcagcccggc ctggacacgt
     6601 cgccgcaatg ccggatcccg aaaccagtcc agcataaaca gtgttcgttc tatacggcca
     6661 atctctctca gagctttagc caacccattt tgtttgtaag cgtgcagcaa gaaccgtatt
     6721 gacgggaatg tgttattcag tcggcagtgc tacgcgccag gggagcagtt cgccgacccg
     6781 gtttatcggc cagtcggcta tgacgtcaag gacatagcgg aggtagcttt ctggctccac
     6841 tccgttcagt ttgcacgtcc cgatcaggct gtacagcagc gctccccgct ctcctccatg
     6901 atccgaaccg aagaacaggt agtttttgcg gcccagactg accatccgca acgcattttc
     6961 agcgatgtta ttgtccgcct cagcccagcc atcatctgca tagtacgtca gcgccggcca
     7021 ctggttcagg gcgtatgcga acgctttcgc cagttctgag tgtcgcgaca gggttttcat
     7081 cttttcacgc agccagcttt ccagggattt cagcagcggt ttcgttttca actgacgttc
     7141 ggcaaggcgc tgttccgccg tcattcccct tatctctgcc tcgatggcgt acagttcgcc
     7201 gatccgtttc agcgcttcct ccgtcagggc tgacggggtg cgaacgtgca catcgtggat
     7261 tttacggcgg gcgtgagccc aacaggcggc ttccgttatc cggccatccc ggtacagctc
     7321 gttgaacccg gcgtatgcat ccgcctgcag tacaccactg aaccccgcaa gatgggtctg
     7381 cggatggatg ccttttctgt ccgggctgta agcgaaccac accgccggcg ccagcgttga
     7441 cccggcgtta cggtcgtcac gaacatacgc ccacaaccgc ccggtcttcg ttttcttatt
     7501 gcctggcaac agcaccggga caggcgtgtc atcaacatgg agcttaccgt cagtcagcac
     7561 atagccatga agcgcctctt ccatcggaga cagcagccgg cagcatgcat ccacccagcc
     7621 cgacagcagt gaacggctca gctccacacc ttgccggccg tatatttctg actggcgata
     7681 cagcggggtg tgctctgcat acttcgaggt cagcacgcgg gccagcagcc ccggtccggc
     7741 gataccccgc tcgatgggcc gcgaaggtgc aggtgcctgc acgatggcat cgcactgagt
     7801 acaggcatgt ttttcccgta ccgtccggat aacccggaag gcgctacgca tcaactccag
     7861 ctgttcggcg gtatcctcgc ccagatagct cagtgaaccg ccgcagttcg ggcagcacgg
     7921 cgccgcaggc aacagtcgct tttcgtcacg gggtagtgat tcagggaacg gcttacgggt
     7981 gcgggtctga cgcaacggac gctgtactgc cgggtcatac accctaccag tcagcgtatc
     8041 gctctctttc tgaagccggt tcagatcggc ttccatttgt gcgatacggc gggagacttt
     8101 ttcggaacga ctgccgaagt tcatccggcg gagtttatcc agctgcgcct gcagatggtc
     8161 tatttcgcgc tcccggttgc taagcttttc ctgcagggcg tggatcagcg cttcctgttc
     8221 ggccaggcgc tgtttcagca ggaagatgtc gtcagaagag atgtcgttca taagcccgta
     8281 ttttaccggg cttattctgt gacaaccagg ataaagagat ttacagcatg gtcagggagg
     8341 tcagcagccg cttaggctgt cgccagtcga taccttccag cagcatcgcc agctgcgcct
     8401 gtgtaaggaa cactttgcca tcacgggctg acggccaggc gaagcgccca cgctccagcc
     8461 gtttggtcag gaggcacagt ccgtcactgg tggaccacag cagtttaacc tgactgccgc
     8521 tgcggccccg gaaaatgaaa acatggccgg acatgggatc gtctttcagc gccgtctgta
     8581 ctttcgcagc caggccgttg aagccatttc tcatatcggt gataccggca accagccaaa
     8641 ttttgatccc ggaaggtaac gggatcatcg cttcagttcc tgtatcagca gagtcaggag
     8701 cttttcgctg acattgccat tgaagcggag cgtcccgtgc cggaacgtta cctcacagct
     8761 gatactgagg gttttcgggt cctctgcgag cgattctggc tgttcggcag ctgcatcgag
     8821 agtcacagga agtagctggg ggctctctga agaaggtaat agcagctttc cctcgcgcca
     8881 ttgttgtcgc catttgaaca acagattggc gttaatgcca ttttcaagag caagttttga
     8941 gatggatatc ccgggttcac aggaggcagc aacgagctgc tgtttaaatt cgggaggata
     9001 attagggcag ccttttcgcc tgccgggagt cacatttttc tgcatatctg acactttggt
     9061 tcccactact tatttggtgg acaccacttt gtctaattcg tcagattctg accagacggt
     9121 tcaggc
//
LOCUS       DAJTVJ010000157         8992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000157 DAJTVJ010000000
VERSION     DAJTVJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.157"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..400)
                     /locus_tag="OO448_003735"
                     /pseudo
     CDS             complement(1..400)
                     /locus_tag="OO448_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(586..3894)
                     /gene="icsA"
                     /locus_tag="OO448_003736"
                     /gene_synonym="virG"
     CDS             complement(586..3894)
                     /gene="icsA"
                     /locus_tag="OO448_003736"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6338842.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGNGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVVGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQNEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4423..5625
                     /gene="virA"
                     /locus_tag="OO448_003737"
     CDS             4423..5625
                     /gene="virA"
                     /locus_tag="OO448_003737"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6338843.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASNSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5804..6491
                     /locus_tag="OO448_003738"
                     /pseudo
     CDS             5804..6491
                     /locus_tag="OO448_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    6084..6200
                     /locus_tag="OO448_003738"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            6493..6859
                     /locus_tag="OO448_003739"
                     /pseudo
     CDS             6493..6859
                     /locus_tag="OO448_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6787..7260
                     /locus_tag="OO448_003740"
                     /pseudo
     CDS             6787..7260
                     /locus_tag="OO448_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            8013..8378
                     /locus_tag="OO448_003741"
     CDS             8013..8378
                     /locus_tag="OO448_003741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338844.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGISADG
                     MYCPECGTVHWPDGVIPPF"
     gene            8426..8855
                     /locus_tag="OO448_003742"
                     /pseudo
     CDS             8426..8855
                     /locus_tag="OO448_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2608 a         2036 c         1895 g         2453 t
ORIGIN      
        1 gttcagttca ggctgtacgc ttccccatgt aatttgcctc gccacttcac cgggctcagg
       61 accccgtagg atcttcaccg aaaaaaatgc atcaccagtt gctttattat ttgttttagg
      121 tagacctgtt actaatttat acctactctg accaagtctg gataagaaag gcacacctgc
      181 acatttctac ttggaaatgt tttcttttac cattttgctc aaaaatttca acatccaata
      241 ttcctcgtac agaactactc aagtcctgaa tagaaaaagt tccagctggt acagtagagt
      301 catatattac tcgaccttgt tgagatacaa caacacgtgc attagtttct gcaataccag
      361 taatctgagg cgcataacca cgaatatttg gcggtagcat tcgttcatcg ctttctaatg
      421 caattcctgt gtaacgccaa gcaacagcgc attctggcgc atgatctggt aaacacgttt
      481 ggcattgatc gcaggcatac catcatgtgc acaaaaaccc accgagcttt tacacggtgg
      541 gtcccagaga aatgcaggac atcaacacgc cctgcatttt tattatcaga aggtatattt
      601 cacacccaaa ataccttggg tgtctctgta actgttatta cccaattgct gagatacgtt
      661 tccccagatg cttaggttat ctgcaagacg cccccccaca ccagttttga gttcaatcac
      721 attacggttg cctatctggt gatttgacat gccattcata attacaccat attgctttga
      781 gctccatttc caattcactt ccaaaaatgg ttcgaactta cgaagagtct ccttatcgat
      841 acaactctga ctaattaagg atgtacgcaa tcccaatttt gcttgaatat tattgacccc
      901 agaggatgtg accatagtgc cattgtgttc aatatagtcg tttgctttta ccccatgcct
      961 agttatctgg acttgtggat tgagatacaa tgcgttgtca atattattat gagcagtcca
     1021 gcgtatggtt gggtagatat agccagcttc caaagctcct gttattcctt tgctggaata
     1081 tttctcacca gttaacccat ctcctttgac tgtattatta aaccaaccgt actgcaacca
     1141 agcatccata tatagacctg ttcgttcttt ttcattctga aaccaagaac tgtacaaacc
     1201 ggcagtataa ccgctcacag ttcctcgtga actataacta gtatacttat tatgagtttt
     1261 gctatcctga tatccataag ctcccataat acccacatgc aagccatcat gatcagtgaa
     1321 gtttgtcttc aaaatatctc caccaatctg ataaaccatc ctgttagctg tggtttttaa
     1381 ttgtctatca gctaaattat gctcattatg tccaccaaca gtacgaatcc acatggtggt
     1441 ttcgctgagc tgacctgtag taggatcaac aatagtgcta ccgtgtcggt catagagaga
     1501 aggactaaac atcgtgttca ttgctatcag gttagcaata taactgccag cttcagggcg
     1561 ccatgtgtga atactacctt cacccaaatt aagtgaagat acaagctgtg aactagctcg
     1621 gcgactactc atttgagtag actcttgatt gctcatctgt tttgattctt gattatccat
     1681 ctgactagtt agataccact tattggtatt cagtccagat acagtgccct gtttcaggcg
     1741 atagtcataa ctaccagcaa caatacggcc tttctgaata aaagcattct tatcagaaga
     1801 atctgtcgaa ataatttgaa caccttcaag agttttatct ccagagcctc cctcatttgt
     1861 cactgcaaca taggtaatac cagacgcatg gcctgcaatt tccagccggt cagttttact
     1921 gttgtcattg ccgagaaccg cgcccaaaga taatattccg ccctttccat gccaattacc
     1981 tttctgaaca taggtctgac cgacatttga cgaactatta ttgagaataa catgaccgtt
     2041 tttctccaga gtcatgttac ctgtgacaat gacggggtca ggcaaaaaag gggcattaat
     2101 attattgatt agcacagtcc cactatttaa taaagtgtta acagtttggt tcatgcctga
     2161 aaaattcaag gtagcacctt tattaacaat aacaccagcg gtacgtgtca tagcttcaac
     2221 tgtccccatt tttagaatgc ctgcattgat gttggtgaaa ccagagtagg tatttttttc
     2281 cgccaaaatg agagttccct cccctgattt tgttagggat tttccgtccc agctatttat
     2341 attatgatgg ctgagattat ctgccagaat agtactaaca gtaagttcag cgttttcttt
     2401 cagattgaac tctccatgac tgtcgccatc tgtatcattc cagctcaaac cgtagatcaa
     2461 tttattctgg tcactgatat agctttttgt atagtcaata gcaccagtag tgagtaacgg
     2521 attgatgata gatatattag aaacactgaa acctgttatc cctttatctg cacttagcag
     2581 tttatagtcc ttctgcgccc agttatttat aatagcttcc acttttacat tgttgccaaa
     2641 tgacatattt ccagaagctt ccagactact gattccagct aaatttaaag aactgttctc
     2701 accaaaagaa acgtcacctg aaacagtgaa tttgttacct gatagtgaaa cagttgtaca
     2761 atcaccagca cataaatcac cttcaatagt tggtgtggtc ccagtgtaat cattgttgat
     2821 aattttcaga atggatgaat tgttaacctg tacatcacca gtaatgacag aaccttcatg
     2881 gagtattaat gaattgtttc caccagtgat gtggatagca ttacctacta cattttgtgt
     2941 tccatcatct tctttacctg aaattatata tccgctatta gcaatggtca tattgctacc
     3001 atttatagca tccccccaat gtccaccatt accgcctaaa atagctccat tgttgattat
     3061 agacaggtta tctcctgtga tagcattacc accgtagcca tcataaccat aaccgtaagc
     3121 agcacctccg ttgcctccag cgaacgtgcc cttattgatt acagagaggc tgcttcctgt
     3181 gatggcatca cctccattac cgccatcacc ctcaccataa ttgttacctc cattaccacc
     3241 ggaaatagtc cctttgttga ttatgggtag attgactcct gtgacggcat cacctccatt
     3301 ccccccatca ctatcactat aactatcgcc accatgtcca cctgaaataa tttctccatt
     3361 tattataaac agattgtctc ctgtaacagc ctcaccacca tcaccgttat ggtcagcacc
     3421 gctaccgccg ttaccaccaa gaatcatgcc ttgattgatt atagacaagt cagatccggt
     3481 aatagagtca ccaccattac cgccacaact attaccatca ttattatcac cagcaccacc
     3541 atgacctgcg ctaatcataa tatcagagtt aatccgtagc ttagttctag atgcatgaga
     3601 ggggttggaa gaagttatat accacgcatc cattccatct tcaccagctc ccaagggaga
     3661 aagtccatca acaggtgtta ataatttttc ataattgtcc tctgaaaaat gaagttcttg
     3721 agtacccgaa agaggaatag caaaagctat tggccccccg agcaacaggg atgcaccaac
     3781 aacaaaagga gaaaaagaca atttgcatgt tttttccttt accgtaggta attctccggc
     3841 ccccccctgt gaacattggg tcatattaca aaaaaatttg tgaatttgat tcatgcacta
     3901 tattatcagt aagtggttga taaaccccta aaaagattcc gttttatccg gaataaaggg
     3961 acgatatatg caaaaatata ttaaacaaag cctcaaccaa aacatgagag ctgtgttata
     4021 tatcaatgca acgggagaaa tttacctgtg tcttgctttg aaaaaaagag ggggaaagct
     4081 atatgttcga taataaatta ataattttac tatgatagtc tgcttcagac cgcaactttt
     4141 cagttctttc tgtacgcttg cccaaagttt aaccactttg tttttctata cacccaatgg
     4201 ttctatctgc cccttgatgt taatccccaa agccaataac atgaatgtat tcatatcaca
     4261 actttcctac cagactctcc aatgcaataa ctgcataatt gtttttagtg gaaatgtatc
     4321 gttttcttaa agagaagaat aacattccat tttattatgt tcccaattta attatattaa
     4381 tatatattgg cattaatagg aaaatacatc aggagaaatc aaatgcagac atcaaacata
     4441 actaaccatg aaagaaatga ctcatcatgg atgtcaactg tcaaatcaac tacagaagta
     4501 agttggaata aattgagttt ttgtgacgtt ttactgaaaa taataacttt cggcatatat
     4561 tctccacacg aaactctagc cgaaaaatat agcgaaaaaa aactaatgga tagtttctca
     4621 ccatcactat cacaagataa aatggacgga gagtttgccc acgcaaacat agatggcatt
     4681 tcaatacggc tatgccttaa caaaggcata tgttcggttt tttatcttga tggagataaa
     4741 atacaatcca ctcaactttc atcaaaagaa tataataact tgttaagttc actccccccc
     4801 aaacaattca acttaggaaa agtacatact attacagctc cggtatcagg gaattttaaa
     4861 actcacaagc ctgcaccaga agttattgaa acagccatta attgctgtac atcgataata
     4921 ccaaacgatg attacttccc cgttaaagac actgatttta atagtgtttg gcatgatatt
     4981 tacagagata ttagagcatc taacagcaat agtacaaaaa tatatttcaa caatattgaa
     5041 ataccactaa aattaattgc agatctaatt aatgagttag gaattaatga attcattgat
     5101 agcaaaaaag agcttcaaat gctaagttac aaccaagtaa ataaaataat caattcaaac
     5161 ttcccccaac aagacctatg ttttcaaaca gaaaaacttt tgtttacttc actctttcaa
     5221 gaccctgcat ttatatctgc actaacatct gcattctggc aatctcttca catcacgtct
     5281 tcctctgtgg aacacatata tgcccaaatt atgtccgaaa acatagaaaa caggcttaat
     5341 ttcatgcctg aacaacgagt tattaacaat tgtggacata ttataaaaat caacgcagtt
     5401 gtccctaaaa acgacactgc aatctctgcc tctgggggga gggcttacga agttagctca
     5461 tcaatattac catcgcacat aacatgtaat ggtgttggta ttaataaaat agaaacaagc
     5521 tatctcgttc atgctggaac acttccaagc agtgaagggc ttcgcaatgc tatccctcca
     5581 gaaagtcgtc aagtatcatt tgccatcata tctcctgatg tttaagccaa tattatattg
     5641 gctgccagac tgtaaaaaaa cgttcttcag aacagctcta taaactatta aaccaaagct
     5701 aaaaggatgg ggaccaatag aatctcagcc catccttttt atatcattat gaaccgcccc
     5761 ggaaatcctg gagactaaac tccctgagaa agaggtaaac aggatgacta aaaatacacg
     5821 tttttcccct gaagtccgtc aacgggcagt tcgtatggtt ctggaaagtc agggcgaata
     5881 tgactcacaa tgggcagcga tttgttccat tgccccaaag attggctgta tactggagac
     5941 tctgcgtgtc tgggtacgcc agcatgagcg ggataccggg ggcggtgagg tgggctcacc
     6001 accgctgaac gtcagcgtct gaaagagccg gaacgtgaaa atcgtgaact gcgccgcagt
     6061 aacaatatcc ttcgcctggc ttccgcttat tttgcaaagg cggagttcga ccgcctctgg
     6121 aaaaaataat gccactgctg gataagctgc gtgagcagta cggggtcgga tcggtatgca
     6181 gtgaactgca tattgccccg tcaacgtatt accactgtca gcaacagcga catcatcatg
     6241 ataaacgcag tgcccgtgcg cagcgcgatg actggctgaa gaaagagata ctgcgcgtat
     6301 acgatgagaa tcatcaggta tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag
     6361 gtatcagagt ggccagatgc actgtggcac gtctcatggc ggttatgggg cttgccggtg
     6421 ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc gcaggcgacc
     6481 gcgtaaaccg tcatcaaggc aatatgccgc gtactccggg tggcccgcag cggctggtat
     6541 acgtggtgtc agcggcggac aaggataaac acacgtcagc agttccgtca gcactgcgac
     6601 agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc ctgacggatg
     6661 aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca
     6721 ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg cacacggcct
     6781 gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga accagaagtg
     6841 ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg gggcgccggg
     6901 ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg ctgatgaaag
     6961 ccgtctgccg caggatcagt gatgcacgtt ttatgagact gctgtggaaa acgccgtgtt
     7021 gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt gatctcgccg
     7081 ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg ctacctgagt
     7141 ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg ccgaagtact
     7201 gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta tgccgatgac
     7261 tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg tgcagccatg cggcacaaga
     7321 gatgagggaa ggcccctcgg tgtcggcgtg caggcgcagg caccaaacca tccactgctg
     7381 ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag gtcggacgtg agccgatcag
     7441 gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat
     7501 gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc
     7561 ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc
     7621 gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc cagagtacca atgcggggta
     7681 cagggacgga gcaccaagcg attgcgcagt aaacatgctt gacaacttaa ttggggcccc
     7741 ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc ggggatttca gtatgaaatg
     7801 aagtatattt aatatacttt acaaaaagtt tgtattagtg gaataaggac aatagtttat
     7861 ccaaactgcc aagccccttt attataagta agatctggta ctaaataagc gttctaaacc
     7921 gccccgagta ggcggcctga cggctgcggc ttgtcatggc ctgtgattac cgttataaaa
     7981 acagacaata tcattgtctt tcaggtagtt atatggcccg ttcagctaaa ccacgcaaac
     8041 gaaaacctgc acctcaaaga agcaaactcc tccgctatgt cgtaaaactt cacgaagatg
     8101 atttttttga cgaagaagaa gctgaggttc tgcgctttga taattttgac gatgccgttg
     8161 agtgttgcgc agacctgaat attccgttct ttgtggatgc cggaaacaaa aaactggtct
     8221 tctggtttgt acgtgttgat gacgaagggt atccggaaat tgcccgctgc acggagcggg
     8281 aatttgcgac aattcctgcc ggtatcagcg ccgacggcat gtattgccct gaatgcggta
     8341 cggttcactg gccggatggc gttattccgc ctttctgatg cttccccgtt ttgccgatat
     8401 ttttcagcag ggaaaccgat ggcttaactg gctggagaag cagccggtgc agatgagccg
     8461 cctggaacat tatgccggtc aggatgaaat tggcctgcgt tacaacagcc accggacaaa
     8521 acgagaagaa aaccttgtga tgagtggcga cgagtttatg gaaaggttct cctggcatgt
     8581 ggcggataag gggttccgga tggtgatacg gggtcctgag tccggtgaag cggcgattac
     8641 tggaagatgt ggtgtacgcc ataacggaga cagtgagaaa aacggcgagg caaatcacaa
     8701 ggagagggat gtatcagcag tgactgaagg ttgacccgct gaagtgcgag ctgtgcggag
     8761 gtcagatgcg gtttacgggg ctgaagaggg gttaccgtct ggcagagcag gttatgatgc
     8821 atgagccgct ggctcgaata cggtggtgcg gctgagagtc gcagagggga agttgcgtcc
     8881 atgttgaggg gaacggagca aaaaaccatc agagatacat tgaatcaatc aatgtaagca
     8941 atattaagtg tctataccga ttacttgatg atcaggttgt gttgaataaa cg
//
LOCUS       DAJTVJ010000158         8818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000158 DAJTVJ010000000
VERSION     DAJTVJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.158"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            802..1044
                     /locus_tag="OO448_003743"
     CDS             802..1044
                     /locus_tag="OO448_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338845.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1374..2303)
                     /gene="fdhE"
                     /locus_tag="OO448_003744"
     CDS             complement(1374..2303)
                     /gene="fdhE"
                     /locus_tag="OO448_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6338846.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2300..2935)
                     /gene="fdoI"
                     /locus_tag="OO448_003745"
     CDS             complement(2300..2935)
                     /gene="fdoI"
                     /locus_tag="OO448_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6338847.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2932..3834)
                     /gene="fdoH"
                     /locus_tag="OO448_003746"
     CDS             complement(2932..3834)
                     /gene="fdoH"
                     /locus_tag="OO448_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6338848.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3847..6897)
                     /gene="fdnG"
                     /locus_tag="OO448_003747"
     CDS             complement(3847..6897)
                     /gene="fdnG"
                     /locus_tag="OO448_003747"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6310..6312),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6338849.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7091..7924
                     /gene="fdhD"
                     /locus_tag="OO448_003748"
     CDS             7091..7924
                     /gene="fdhD"
                     /locus_tag="OO448_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6338850.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8077..>8818
                     /locus_tag="OO448_003749"
     CDS             8077..>8818
                     /locus_tag="OO448_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR6338851.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLI"
BASE COUNT         2112 a         2228 c         2262 g         2216 t
ORIGIN      
        1 ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt cttcgttggc
       61 accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt gtagagctga
      121 gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg agcgattttt
      181 gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa aatctatagc
      241 tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt catttactgc
      301 tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga aggaggctat
      361 aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta atttagaaaa
      421 acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa gaccggttgg
      481 tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct ggtgctgtta
      541 ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc cctgaatata
      601 atatattcat agcacccata actcatcctg ggcgataact aaaccatttt tgagtaataa
      661 atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa taaaaaatga
      721 tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca tagattcaaa
      781 aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg cagaatttta
      841 ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca gcgtaatctt
      901 cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca atcgcaaaca
      961 gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt cgaatatcag
     1021 cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt gctgacaacg
     1081 tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa agtcatgcaa
     1141 attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag gcaatcatac
     1201 gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag aacgaggcaa
     1261 acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat ggtgtcgtca
     1321 caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag agattactct
     1381 ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc ttgctccatt
     1441 cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt cggttctttt
     1501 tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc tttaatcgcg
     1561 gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca gttgctgcat
     1621 tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta acgcagaccc
     1681 tgagtggtgc caatttgcac catgctggac accggcatag agccacatac cgggcaatat
     1741 tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc catctgcgcc
     1801 cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac ggacgagaaa
     1861 tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc cttctccaga
     1921 ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc aattagcgcc
     1981 atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag cgggggcttg
     2041 ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg atggtcgtac
     2101 agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc acccagcgga
     2161 ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt cttgagccga
     2221 gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc cagctcatct
     2281 tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg acctcacggt
     2341 accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc accatggcgg
     2401 taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc acaattaacg
     2461 ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt gagaaagcag
     2521 gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc aggaaaataa
     2581 tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca cctacttcct
     2641 cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga ttgattaggt
     2701 tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc acgccgacaa
     2761 acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac cagttgaagg
     2821 acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag gcggtgatcc
     2881 agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt ttcatttgcg
     2941 ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt tcggaccgac
     3001 acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag ccgcgagcgg
     3061 tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct ccggcaagcc
     3121 atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg taccaccgac
     3181 gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct cagcaacgcg
     3241 ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc ccgttgggca
     3301 ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca gcgtacattt
     3361 gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc ccgcaatgca
     3421 ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg catactgaat
     3481 aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt gcatacagcc
     3541 gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga agcgcatcac
     3601 cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt tattgccgac
     3661 ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac agccgataca
     3721 ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct gaggcgcggg
     3781 ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag ccatatgtcg
     3841 tctccgttac accttttcca cattcacaag gaaggactta aactccggcg tctgcgtgtt
     3901 cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag ctacgccttc
     3961 atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg ctttcagcgt
     4021 gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg aggagacttt
     4081 cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga tttccacaaa
     4141 ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt ggaagtgctc
     4201 ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca atgcttcggc
     4261 gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca gcgggttagt
     4321 tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg ccatcttatc
     4381 gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga cgccgctgcc
     4441 cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc cgtcccattt
     4501 cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg cgcggttata
     4561 cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac cgaggccaga
     4621 cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc taccggcgaa
     4681 aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg agctaagttg
     4741 ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg ccagcgcctt
     4801 accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg cgtagttcca
     4861 ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca tcttgcgcaa
     4921 gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg gggcgtccgc
     4981 acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt tctcttctgc
     5041 gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt caacttcgtt
     5101 cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt cgatggtgac
     5161 gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga atgaggcaac
     5221 agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca tctcgaagta
     5281 ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc agctattttc
     5341 cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt tcgggtagtt
     5401 gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg taaggtaggt
     5461 ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc tctgcgacag
     5521 cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt taacgccgcc
     5581 gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac gaatgttttg
     5641 cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag ttttatcgtg
     5701 agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg gcgtaccaca
     5761 gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca agttccacac
     5821 gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt ccagttcgta
     5881 agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga acaggccatc
     5941 ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt cggtgtattc
     6001 gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga aagcaatgtc
     6061 agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg taaagcgagg
     6121 atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc agcggaaccc
     6181 gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg cgttcttgat
     6241 gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     6301 tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg cgcgggagaa
     6361 tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca tcccggtgga
     6421 gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag catcgcggtc
     6481 ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt gctgccattt
     6541 atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt ggatgaaatc
     6601 caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat ccggatcgcc
     6661 ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc tgtacatcaa
     6721 cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg tgcgcagcag
     6781 tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg ccgctgccgt
     6841 ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga cctgcatgga
     6901 ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg tgtgcaggga
     6961 gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc tcaatttccc
     7021 tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt tcttatctgg
     7081 ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa atatcacagg
     7141 tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt tagatgaggt
     7201 cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg tgatgatggc
     7261 gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg ggataataga
     7321 aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc ttgaagtaca
     7381 aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg cgctggcggg
     7441 acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa aaccggtgca
     7501 gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat tacgtcatct
     7561 caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg cctggatgtt
     7621 gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg cgctggacaa
     7681 actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg tactggtttc
     7741 cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag agattttgtt
     7801 tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta atctgacact
     7861 ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc gtttgagcaa
     7921 ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat ttcgtgaatt
     7981 ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttttc atttattatg
     8041 ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt attatcttcc
     8101 gctattatag tcgccatcat ggccctcggt ctgacgggtt gtgatgataa aaaagccgaa
     8161 acagaaacgc ccccgcctgc caatagtcaa cctgccgcac cagctcctga agctcccgtt
     8221 gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg tggtcgatga acaagcggtt
     8281 ttcgacgaaa aaatggatgt ctatatcaag tgctacaaca agttacagat ccctgtacag
     8341 aacagtctgg cgcgttatgc tgactggctg aaagatttta aacaggggcc taccggtaaa
     8401 gagagtactg tttatggcat ctacggcatt agtgaatcca acctcgctga gtgtgaaaaa
     8461 ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac ctattgatgg cgtggcggtg
     8521 agttatattg atgctgccgt ggcgttgggt aataccatta acgaaatgga taaatattac
     8581 acccaggaaa attataaaga cgatgcgttt gcgaaaggga aaacgctgca ccagacattc
     8641 ttaaaaaatc tggaatcctt tgaacctgta gcagaatctt atcatgcggc gattcaggaa
     8701 attaatgata agcgccagct tgcggaactg aaaaatattg aagaaagaga agggaaaaca
     8761 ttccactact actctctggc agtcatgatt tcagcgaaac aaattaataa cctgattt
//
LOCUS       DAJTVJ010000159         8810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000159 DAJTVJ010000000
VERSION     DAJTVJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.159"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..766)
                     /locus_tag="OO448_003750"
                     /pseudo
     CDS             complement(1..766)
                     /locus_tag="OO448_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1045..1998
                     /gene="tal"
                     /locus_tag="OO448_003751"
     CDS             1045..1998
                     /gene="tal"
                     /locus_tag="OO448_003751"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6338852.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2113..2700
                     /gene="mog"
                     /locus_tag="OO448_003752"
     CDS             2113..2700
                     /gene="mog"
                     /locus_tag="OO448_003752"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6338853.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OO448_003753"
     CDS             complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OO448_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6338854.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OO448_003754"
     CDS             complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OO448_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6338855.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(4189..4593)
                     /locus_tag="OO448_003755"
     CDS             complement(4189..4593)
                     /locus_tag="OO448_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6338856.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4970..6886
                     /gene="dnaK"
                     /locus_tag="OO448_003756"
     CDS             4970..6886
                     /gene="dnaK"
                     /locus_tag="OO448_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6338857.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6975..8105
                     /gene="dnaJ"
                     /locus_tag="OO448_003757"
     CDS             6975..8105
                     /gene="dnaJ"
                     /locus_tag="OO448_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6338858.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OO448_003758"
     CDS             complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OO448_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6338859.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2333 a         2187 c         2259 g         2031 t
ORIGIN      
        1 aaggtatatc ccgccgcgcc gcctgccgct tcctgccagc caaaagcact ctcaaaaata
       61 gaccaaatga cgtggggaag ttgcccgata tttattacgc aaatcaccat gctggtcagc
      121 acccagatta tcgccatcaa cgggacaaac ccctgcatga gccgggcgac gccatgaaga
      181 ccgcgagtga ttgccagcag agcaaagaca gcgagaataa tgcctgtcac cagcggggga
      241 aaatcaaaag aaaactcagg gctcgggcaa cggcgttcgc ctgaactccg ctgaaaatta
      301 tgccgtaggc gatgagcaaa aagacggcga acagaacgcc catccaacgc atccccagcc
      361 cgcgcgccat ataccatgcc ggtcctccac gaaactgtcc attgacgtca cgttctttat
      421 aaagttgtgc aagggaacat tcggcaaacg aggtcgccat gccgataaac gcggcaaccc
      481 acatccaaaa gacggctcca ggtccaccgg cggtaatagc cagcgcaacg ccggccaggt
      541 tgccgctacc cacgcgcgcc gcaagactgg tacacaatga ctgaaatgag gttaaaccgc
      601 ctggctgtgg atgaatgcta tttttaagac ttttgccaaa ctggcggatg tagcgaaact
      661 gcacaaatcc ggtgcgaaaa gtgaaccaac aacctgcgcc gaagagcagg taaatcatta
      721 ccgatcccca aaggacgctg ttaataaagg agaaaaaatc tggcatgcat atccctctta
      781 ttgccggtcg cgatgacttt cctgtgtaaa cgttaccaat tgtttaagaa gtatatacgc
      841 tacgaggtac ttgataactt ctgcgtagca tacatgaggt tttgtataaa aatggcgggc
      901 gatatcaacg cagtgtcaga aatccgaaac agtctcgcct ggcgataacc gtcttgtcgg
      961 cggttgcgct gacgttgcgt cgtgatatca tcagggcaga ccggttacat ccccctaaca
     1021 agctgtttaa agagaaatac tatcatgacg gacaaattga cctcccttcg tcagtacacc
     1081 accgtagtgg ccgacactgg ggacatcgcg gcaatgaagc tgtatcaacc gcaggatgcc
     1141 acaaccaacc cttctctcat tcttaacgca gcgcagattc cggaataccg taagttgatt
     1201 gatgatgctg tcgcctgggc gaaacagcag agcaacgatc gcgcgcagca gatcgtggac
     1261 gcgaccgaca aactggcagt aaatattggt ctggaaatcc tgaaactggt tccgggccgt
     1321 atctcaactg aagttgatgc gcgtctttcc tatgacaccg aagcgtcaat tgcgaaagca
     1381 aaacgcctga tcaaactcta caacgatgct ggtattagca acgatcgtat tctgatcaaa
     1441 ctggcttcta cttggcaggg tatccgtgct gcagaacagc tggaaaaaga aggcatcaac
     1501 tgtaacctga ccctgctgtt ctccttcgct caggctcgtg cttgtgcgga agcaggcgtg
     1561 ttcctgatct cgccatttgt tggccgtatt cttgactggt acaaagcgaa tactgataag
     1621 aaagagtacg ctccggcaga agatccgggc gtggtttctg tatctgaaat ctaccagtac
     1681 tacaaagagc acggttatga aaccgtggtt atgggcgcaa gcttccgtaa catcggcgaa
     1741 attctggaac tggcaggctg cgaccgtctt accatcgcac cggcactgct gaaagagctg
     1801 gctgagagcg aaggggctat cgaacgtaaa ctgtcttaca ccggcgaagt gaaagcgcgt
     1861 ccggcgcgta tcactgagtc cgagttcctg tggcagcaca accaggatcc aatggcagta
     1921 gataaactgg cggaaggtat ccgtaagttt gctattgacc aggaaaaact ggaaaaaatg
     1981 atcggcgatc tgctgtaatc attcttagcg tgaccgggaa gtcggtcacg ctacctcttc
     2041 tgaagcctgt ctgtcactcc cttcgcagtg tatcattctg tttaacgaga ctgtttaaac
     2101 ggaaaaatct tgatgaatac tttacgtatt ggcttagttt ccatctctga tcgcgcatcc
     2161 agcggcgttt atcaggataa aggcatccct gcgctggaag aatggctgac atcggcgcta
     2221 accacgccgt ttgaactgga aacccgctta atccccgatg agcaggcgat catcgagcaa
     2281 acgttgtgtg agctagtgga tgaaatgagt tgccatctgg tgctcaccac gggcggaact
     2341 ggcccggcgc gtcgtgacgt aacgcccgat gcgacgctgg cagtagcgga ccgcgagatg
     2401 cctggctttg gtgaacagat gcgccagata agcctgcatt ttgtaccaac tgcgatcctt
     2461 tcgcgtcagg ttggtgtgat tcgcaaacag gcgctgatcc ttaacttacc cggtcagccg
     2521 aagtctatta aagagacgct ggaaggtgtg aaggacgctg cgggtaacgt tgtggtacac
     2581 ggtatttttg ccagcgtacc gtactgcatt cagttgctgg aagggccata cgttgaaacg
     2641 gcaccagaag tggttgctgc attcagaccg aagagtgcaa gacgcgacgt tagcgaataa
     2701 aaaaatcccc ccgagcgggg ggatctcaaa acaattagtg ggattcacca atcggcagaa
     2761 cggtgcgacc aaactgctcg ttcagtactt cacccatcgc cagatagatt gcactggcac
     2821 cacagatcag accaatccag ccggcaaagt ggatgattgc ggcgttaccg gcaatgttac
     2881 cgatcgccag cagggcaaac agcacagtca ggctaaagaa aacgaattgc agaacgcgtg
     2941 cgcctttcag cgtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga
     3001 caccaaggaa ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg
     3061 caaccagcgt caaccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc
     3121 cttttttgta ctccagcaga ccggcaaaaa tttgcgcgat gccgccgtag aaaatgccca
     3181 tggcaagaat aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg
     3241 tggtcatgcc gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca
     3301 taattcctca aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taacagtagt
     3361 gttttcaggg cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg
     3421 aaaatgaaaa ttttccccgg tttacggtgt cagacctgag tggcgctaac catccggcgc
     3481 aggcaggcga tttgtattac ggctggaatt gtcacgcgat aggcactgcc gctgaccgct
     3541 ttaaccccat ttaatgctgc gcccacaggg cctcccagac ccgcgccgcg cagcaaacca
     3601 tgcccaagca cgctcattgc tgcgtgggtg cgtaaaatgc gggtcaattg gctggaaagc
     3661 aaatgcgaca cgccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc
     3721 agctcattca ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccata
     3781 ttcttccagg tgttacgcag aaattgttcc agtaactgtt gctcaatctc aaacgtagac
     3841 atctctttgt cggctttcag cttcaatcgt tttgaaacat cgagcaaaat ggcccgatac
     3901 aatttaccgt gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct
     3961 ccagcaatca gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac
     4021 agttcgttgc gcatcagtac gctggagagg cgagttttgc ctttttcatt atgggtgagc
     4081 aatcgggcga aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa
     4141 tcattcaggt aattaacatt cattttttgt ggtttctata ttctggcgtt agtcgtcgcc
     4201 gataattttc agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt
     4261 tgagacgcag cgtttattgt cgttatcact gttaatgttg atccagtcag tggtttgccc
     4321 ttcttttatt tctgaaggaa tattcagact ctgactggcg ctacgggcgg ctttgaaata
     4381 aaccgatgca ccgcttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac
     4441 aatgcgacaa acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat
     4501 gacgccgagg agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc
     4561 caggctggcg gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag
     4621 agggatgaat acccactagt ttactgctga taaagagaag attcaggtac gtaatctttt
     4681 ctttttatta caattttttg atgaatgcct tggctgcgat tcattcttta tatgaataaa
     4741 attgctgtca attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa
     4801 aaaatttttg catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc
     4861 agtgagtgag tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat
     4921 tacagactca caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat
     4981 aattggtatc gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccactcc
     5041 tcgcgtgctg gagaacgccg aaggcgatcg caccacgcct tctattattg cctataccca
     5101 ggatggtgaa actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa
     5161 caccctgttt gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg
     5221 tgatgtttcc atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga
     5281 agttaaaggc cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa
     5341 gaaaaccgct gaagattact tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc
     5401 atactttaac gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga
     5461 agtaaaacgt atcatcaacg aaccgaccgc agctgcgctg gcttacggtc tggacaaagg
     5521 tactggcaac cgtactatcg cggtttatga cctgggtggt ggtactttcg atatttctat
     5581 tatcgaaatc gacgaagttg acggcgaaaa aaccttcgaa gttctggcta ccaacggtga
     5641 tacccacctg gggggtgaag acttcgacag ccgtctgatc aactacctgg ttgaagaatt
     5701 caagaaagat cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga
     5761 agcggcagaa aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc
     5821 gtacatcact gcagatgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa
     5881 actggaaagc ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact
     5941 gcaggacgct ggcctgtccg tatctgatat cgacgacgtt atcctcgtgg gtggtcagac
     6001 tcgtatgcca atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga
     6061 cgttaacccg gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg
     6121 tgacgtaaaa gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat
     6181 gggcggtgtg atgacgacgc tgatcgcgaa aaataccact atcccgacca agcacagcca
     6241 ggtgttctct accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga
     6301 acgtaaacgt gcagctgata acaaatctct gggtcagttc aacctggatg gtatcaaccc
     6361 ggcaccgcgc ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatctt
     6421 gcacgtttcc gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc
     6481 ttctggtctg aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga
     6541 agctgaccgt aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca
     6601 cagcacccgt aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc
     6661 tatcgagtct gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat
     6721 cgaagcgaaa atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca
     6781 gcaacatgcc cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga
     6841 cgatgttgtc gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaacg
     6901 ggtaattata ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg
     6961 acaatttaaa aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag
     7021 cggaagagcg tgaaatcaaa aaggcctaca aacgcctggc catgaaatac cacccggacc
     7081 gtaaccaggg tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag
     7141 ttctgaccga ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc
     7201 aaggtggcat gggcggcggc ggttttggcg gcggcgcaga cttcagcgat atttttggtg
     7261 acgttttcgg cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg
     7321 atttacgcta taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga
     7381 tccgcattcc gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta
     7441 cacagccgca gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat
     7501 ttttcgctgt acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc
     7561 cgtgcaacaa atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa
     7621 tcccggcagg ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg
     7681 agcatggcgc accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt
     7741 tcgagcgtga aggcaacaac ctctattgcg aagtcccgat caacttcgct atggcggcgc
     7801 tgggtggcga aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg
     7861 aaacccagac cggtaagctt ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg
     7921 gcgcacaggg tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaga
     7981 agcagaaaca gttgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggtgagcaca
     8041 acagcccgcg ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc
     8101 gctaacctcc ccaaaagcct gcccgtgggc aggcttgggt aaaattaggg tgcgttgaag
     8161 atatgcgagc acctgaaaag tggcggggat cactccccgc cgttgctctt actcggattc
     8221 gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc
     8281 ttttctcgtt accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat
     8341 catcgcctta tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac
     8401 tctacatgtg ttcagcatat agggggcctc gggttgatag taaaatatca ctcggggctt
     8461 ttctctatct gccgttcagc taatgcctga gacagatagc ctcaagcacc cgccgctatt
     8521 atatcgctct ctttaaccca ttctgtttta tcgattctaa tcctgaagac gcctcgccta
     8581 tttttgtggc gtaatttttt aatgatttaa ttatttaact ttaatttatc tatttatcgc
     8641 aattattgac gacaagctgg attatttttg aaatattgtc ctaacaaata acgccgactg
     8701 acaagaaatt aattattact tttcctaatt aatccctcag aaatcctgac cttaagctat
     8761 gattatcagg gcttagggtc acccgttagc gctgatatga ctttccgtgt
//
LOCUS       DAJTVJ010000160         8762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000160 DAJTVJ010000000
VERSION     DAJTVJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.160"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1343
                     /gene="rtcR"
                     /locus_tag="OO448_003759"
                     /pseudo
     CDS             1..1343
                     /gene="rtcR"
                     /locus_tag="OO448_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1325..2083)
                     /locus_tag="OO448_003760"
     CDS             complement(1325..2083)
                     /locus_tag="OO448_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6338860.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2100..2930)
                     /gene="glpG"
                     /locus_tag="OO448_003761"
     CDS             complement(2100..2930)
                     /gene="glpG"
                     /locus_tag="OO448_003761"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6338861.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(2975..3301)
                     /gene="glpE"
                     /locus_tag="OO448_003762"
     CDS             complement(2975..3301)
                     /gene="glpE"
                     /locus_tag="OO448_003762"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6338862.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3491..4996
                     /gene="glpD"
                     /locus_tag="OO448_003763"
     CDS             3491..4996
                     /gene="glpD"
                     /locus_tag="OO448_003763"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6338863.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5048..5653)
                     /locus_tag="OO448_003764"
     CDS             complement(5048..5653)
                     /locus_tag="OO448_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338864.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5656..6423)
                     /locus_tag="OO448_003765"
     CDS             complement(5656..6423)
                     /locus_tag="OO448_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338865.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6426..7136)
                     /locus_tag="OO448_003766"
     CDS             complement(6426..7136)
                     /locus_tag="OO448_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338866.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7152..8657)
                     /locus_tag="OO448_003767"
     CDS             complement(7152..8657)
                     /locus_tag="OO448_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6338867.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2260 a         2271 c         2162 g         2069 t
ORIGIN      
        1 aagaggttta cgcctgcctg catgatttcg cccgtggtta cgagtttcag ccagaaaaag
       61 aagactattt aattcacatc accaccggca cccacgtcgc gcagatttgc tggtttctgc
      121 tggcggaagc gcgttacctg cccgcccggc tgatacaatc ttcacctccg cgcaaaaaag
      181 aacagccgcg cggcgcaggt gaagtgacga ttatcgatct cgatttaagc cgttataacg
      241 ccatcgccag ccgctttgcc gaggaacgcc agcaaacgct tgattttctt aagtccggca
      301 ttgccacgcg taacccccac ttcaaccgca tgattgagca gatcgaaaaa gtggcgatca
      361 aatcccgcgc gccgattctg cttaacggtc caaccggcgc gggcaagtca tttctggcgc
      421 gacgcatctt cgagttaaaa caggcgcggc atcagtttag cggcgcgttt gtggaagtga
      481 actgcgccac cctgcgcggc gataccgcca tgtcgacgct gtttggtcat gtaaaaggcg
      541 cgtttaccgg ggcgcggtaa tctcgtgaag gtttattacg cagcgccaac ggcggaatgt
      601 tgtttcttga tgagattggc gaactgggcg cagacgaaca ggcaatgctg ctaaaagcca
      661 ttgaagagaa aaccttttac ccgtttggca gcgatcgcca ggtgagcagt gattttcagc
      721 ttatcgccgg aacggtgcgc gatttgcgcc agctggttgc cgaaggcaaa tttcgcgaag
      781 atctgtacgc gcggatcaat ctctggacct tcaccctgcc gggtctacgc cagcgccagg
      841 aagatattga accgaacctt gattatgaag tggagcgcca cgccacactc actggcgaca
      901 gcgtgcgttt taacaccgaa gcgcggcgcg cctggttggc ttttgcgacc tctccccagg
      961 caacatggcg cggtaacttt cgcgaacttt ctgccagcgt cacgcggatg gccacctttg
     1021 ccactagcgg acgcatcact ctggacgtgg ttgaagatga gataaaccgt ctgcgctata
     1081 actggcagga gagccgcccc tccgcgctta cggcgttgct gggcgcagag gcagaaaaca
     1141 tcaatctctt cgaccgtatg caactggaac acgttatcgc tatctgccgc taggcaaagt
     1201 cgctttctgc agccggacga cagctttttg atgtttcgcg ccagggcaaa gccagcgtca
     1261 atgacgcgga tcggctacgc aaatacctgg cgcgttttgg tctgacgtgg gaagccgtgc
     1321 aggatcagca cagctccagt tgaatatgat ggtccgtcag cacctgcatt acgcttactg
     1381 gcggcggggc gtcggtgtag acggcatcta ccatgctgat gctgcccata ttgaccatcg
     1441 cgttacggcc aaatttcgag tgatcgacaa ccagcataac gtggcgcgag ttctcaataa
     1501 tggcgcgctt ggtgcgaact tcgtggtaat cgaactccag cagcgagccg tcgctatcga
     1561 tgccgcttat ccccagaatg ccgaaatcaa ggcggaactg ggagataaaa tcgagcgtcg
     1621 cttcgccaat gatcccgcca tcgcggctgc gtaattcgcc accggcgaga ataatgcgga
     1681 aatcttcttt taccatcaac gtgttagcaa cattgagatt gttggtgaca atacgcaaat
     1741 tgctgtgatt aagcagcgcg tgcgccaccg cttccggcgt ggtgccgata tcgataaaca
     1801 gtgtcgagcc gttggggatt tgctccgcca ctttgcgggc aatcctctct ttttcttcgg
     1861 tctgggtggc tttgcgatcg tgccacggcg tgttaaccga actggaaggc agcgccgcac
     1921 cgccatggtg acgcaggatc aggttttgct ccgccagctc attgaggtcg cggcgaatag
     1981 tctgcgggct gacggagaaa tgctctacca gctcttcagt actgacataa ccctgctgtt
     2041 taaccagttc gataataccg ttgtgacgtt gtgtttgttt catttataaa tccctggaat
     2101 tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca tcgctaaacc cacggctaac
     2161 ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca aatcaaacca tccggcgaca
     2221 atccagatca gcgcaaagat aattaaccca cgttgcaggt aaatgccact ttgcggatcg
     2281 cgttcaccac gtagccagac gtagcccatc agcgcataca ccacgccaga aagcccgcca
     2341 aaccacggcc cgctgaattt ttgctgcaca tagccgctta acagggcgct aatgagagta
     2401 ataacaatta gcttaccgct accgaggcgt ttttccaccg caccgccgag ataccaccac
     2461 cagagcaggt taaagaggat atgcatcagc gagaagtgca ttaacgcgtg ggtgaagtaa
     2521 cgccagaact caaatttcag tgctggatcg aatggccagg ccagccataa catcacttcc
     2581 tgatcgccga gaatttgcat ggcaataaac accaccacgc aggcgatcat catcacccag
     2641 gtcaccggac ctgcgcgttc acgcaaggcg gcaaagaaag gataacggcg ataatgcagg
     2701 ccactgccgg tatggcctgc ctgccagctg gccgccagat aacgcggatc tgccgggttt
     2761 tcgagaaaac gcgccagctc cgcccgtacg cgctcggcct gggactcatc cgccagccag
     2821 acatcgcttt ggttatgttg ttgaatcgtg aggataacac cctgcgtcgc catgtaatca
     2881 acaaacgcct gcgccacgcg ggggttagca aaagaggtaa tcatcaacat cgttgctgtc
     2941 gcttattcca cacaaaaggg gacagtataa agcgttacgc gccgtacgcc acctctgcgg
     3001 gaaactgacg ttgccaggct tcaaagccgc cgtcaatgct atagaccaca tcgtagccct
     3061 gttgcagcag atactgcgcc gcgcctttgc tgctattgcc gtgataacac atcaccatca
     3121 ccggagtgtc aaagtcgtta tcacgcataa acgcgcccag cgtgtcgttg gttaaatgga
     3181 aagcctgcac cgcatgcccc atagcgaaac tctgtggatc gcgaatatcg accagcaccg
     3241 cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt aatacattcg aactgatcca
     3301 tgcgtctctc tttctttaca aacaagtggg caaatttacc gcacagttta cgtcgaagcg
     3361 gcagataaac gccataatgt tatacatatc actctaaaat gttttttcaa tgttacctaa
     3421 agcgcgattc tttgctaata tgttcgataa cgaacattta tgagctttaa cgaaagtgaa
     3481 tgagggcagc atggaaacca aagatctgat tgtgataggt ggcggcatca atggtgctgg
     3541 tatcgcggca gacgccgctg gacgcggttt atccgtgctg atgctggagg cgcaggatct
     3601 cgcttgcgcg acctcttccg ccagttcaaa actcattcac ggtggcctgc gctaccttga
     3661 gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa cgtgaagtgc tgctgaaaat
     3721 ggctccgcat atcgccttcc cgatgcgttt tcgcctgcca catcgtccgc atctgcgccc
     3781 ggcgtggatg attcgcattg gtctgtttat gtacgatcat ctgggtaaac gcaccagcct
     3841 gccgggatca actggtttgc gttttggcgc aaattcagtg ttaaaaccgg aaattaagcg
     3901 cggattcgaa tattctgact gttgggtaga cgacgcccgt ctggtactcg ccaacgccca
     3961 gatggtggtg cgtaaaggcg gcgaagtgct tactcggact cgcgccacct ctgctcgccg
     4021 cgaaaacggc ctgtggattg tggaagcgga agatatcgat accggcaaaa aatatagctg
     4081 gcaagcgcgc ggcttggtta acgccaccgg cccgtgggtg aaacagttct tcgacgacag
     4141 gatgcatctg ccttcgcctt atggcattcg cctgatcaaa ggcagccata ttgtggtgcc
     4201 gcgcgtgcat acccaaaagc aggcctacat tctgcaaaac gaagataaac gcattgtgtt
     4261 cgtgatcccg tggatggacg agttttccat catcggcacc accgatgtgg aatacaaagg
     4321 cgatccgaaa gcggtgaaga ttgaagagag tgaaatcaat tacctgctga aagtgtataa
     4381 cacgcacttt aaaaagcagt taagccgtga cgatatcgtc tggacctact ccggtgtgcg
     4441 tccgctgtgt gatgatgagt ccgactcgcc gcaggctatt acccgtgatt acacccttga
     4501 tatccatgat gaaaacggca aagcaccgct gctgtcggta ttcggcggta agttgaccac
     4561 ctaccgcaaa ctggcggaac atgcgctgga aaaactgacg ccgtattatc agggcattgg
     4621 cccggcatgg acgaaagaga gcgtgctgcc gggtggtgcc attgaaggcg accgcgacga
     4681 ttacgcagct cgtctgcgcc gtcgctatcc attcctgact gaatcgctgg cgcgtcatta
     4741 cgcacgcact tacggcagca acagcgagct gctgctcggc aatgcggggg cgataagcga
     4801 cctcggggag gatttcggtc atgagttcta cgaagcggaa ctgaaatacc tggtggatca
     4861 cgaatgggtc cgccgcaccg acgatgccct ttggcgtcgc acaaaacaag gcatgtggct
     4921 gaatgcggat caacaatctc gtgtgagtca gtggctggtg gaatatacgc agcagaagtt
     4981 gtcgctggcg tcgtaaggat gatgcccggt tgaagtgcaa ccgggctatt tggattcctt
     5041 agactattca ttgtaaataa ttgaataacg gcccctgagc taaaatcgcc aaacttaagc
     5101 acgttaccca atcgccccac ttcttcctct ggtttctatt ttttctgcac caatggcaat
     5161 aatatcccaa tgcaaaacca agctgtgcac taatcgggcc gacaaaatca gtccaaggct
     5221 gcggccaaaa aggggaaaaa ttcaggggag gccagacaaa cgtcagaaag aaatttcgga
     5281 tattttccag caacgctgat ttctctggag aaatataaag aatgaaattc gagagaatta
     5341 accagaaata gaccggcgaa aagaccagcc agcaatgcag tctgtttttt aattcaggag
     5401 gcgttctgcg ggagaattgc caaccgatgc cagccattac actgaaaaaa agtgcatatt
     5461 caaccattaa ctcaagttgt aattcctgaa gagaaatacg cacaaccagg agcggaaagg
     5521 agaatattat ttctgccaga aaagttaacg tgaatataag cacggctaag acaataaccg
     5581 tcggccatac caataatggc acgataacaa aaccgagaat tacaggggga atataaagcc
     5641 ggaatttaat catttttatt ttcctggtga ttcggatgat gcgtcataca tcgcgcataa
     5701 acactcatca attatccagt catcttccca gacctgatat tctgtcattg gttcgggctg
     5761 ttttagcggg ttctcaatcg cttgccgcgc tctttctttc agtaaaggat gattatttct
     5821 agcccaggca ttgatttggg tggtaatggc gttatcgata tcgtcctggt tatctctgat
     5881 ataatcctca caatatctgt tcaataaaca ggcaagagaa acggtcgacc aaaagccggg
     5941 aactgaatta atggcatcca gcaatggcgc aagagcgata tcggcacgga aaaacatttc
     6001 cagcatatac tgtaattcga cagcttcatc gccggatacc caatcagtca tatactggca
     6061 ggcaaatcgc tgtaaaattg cccgttcatc cgcacgccag ttttctttgt tggcaaggtt
     6121 gatacggcgc agagaaaaaa ctacgtgaat attgtcaaaa tgcccctgtg caacaaattc
     6181 cagtaaacgc ggcagaaaat agcggacctc atcgctattt tgcggatcag gccctgggct
     6241 actattccag gcattgatta aggaatacgg gatcgctcgt aacgatgtac tccgtaatgc
     6301 cttttgttcc tgctgactca ggcaatattc acaacaaacc acaaatttct gcggcacccg
     6361 ataacgacga aaggtgtgat aaatctgctc aattaatgcc tgcatggcgg ggctgggttg
     6421 catagttacc ccctcgcccg ctgatcgcgt gtaaacggtt gatagacaaa acgagcattc
     6481 gccgcgttca ggcgaataag ggcgctacgc gaaagggaaa agctggggcg aatgcaacta
     6541 ttgcagtctc gacagctttc ctctgcgcaa ttttccactg agtaatgacg cgtgtagagt
     6601 ttcagttcgg cctgcttatc gcgaaatccg cgtaaactgt tattgttatg aaatttccag
     6661 gcatctttaa agacctgaaa gatatagaca tcctcaatcc aggagtcaca ttttttaagc
     6721 gggcgctttg ctattatcac ccgctcatcc ggatttagca tcgccgtaaa ttgtacggtg
     6781 acgtcatttg tcgtgttcag gtcacattga acatctggta catcactgat ataggggtca
     6841 aatccaaaat cagcaccaca ggcacaaaga cggctaatgg tggcggtatc gagggaaaat
     6901 cccggccata ccttgctgga ggttaagcca atatttaatg tgacatcata tcgaagcaat
     6961 ccctcaagac cgtcagcacc gacgaacttt tccccttcat ccaaggcctg gttaataagt
     7021 tgctgaacaa tgccttcatc ccagtatgcc gatttgcagt tggcaatcgt aatatccacg
     7081 cctgtgaagg gatcagtacc ggcaaggctt gcaaataatg tattaactaa ttccattttt
     7141 attatccagc tttaaggtag cgtcttatac atcgggacat aacctacatc ttccaccaaa
     7201 ctctgtccct gcggcgttaa aaaccactcc aacaattttt gtgtctctga cgtcgtattt
     7261 tcccgcgtta ccataaaggc atcgacgata tagggatatt tgccgttgcg aatgttttcg
     7321 gcagtcggtg caataccgtt aatcgctagc agttttatat ttttatcggc gttcatttgt
     7381 gttgcgtaat aacggaaggt gtaacctatt gcgttgtttg tattacggta ttcggcaacg
     7441 actttaatca ttccctccat catacttgcc acttctgttt cctgcggcga gatcatgcgg
     7501 acatttttca tgacctggga ttgcatcacc gtctggctac cagagtcttc cgggcgttgc
     7561 caggtctgga tctcctgatc gttaccgcca acagtacgcc agttggtaat tgcaccactg
     7621 aagatgtcac gcacctgttg ttcagtcagg gaattaaccg gattatccgc attgacgatg
     7681 aaaacaaacg cttcacgggc aaaaggcgtg tacagcaaag taacgcccga ttcctcagcg
     7741 cgttttttct gcccaccgga aggttgcgcc acaaaaataa tatcagcatc acctttaaca
     7801 atcctgttat acgcatcggg ggtacgagag ctctccagat attccctggt gtgaaaatct
     7861 tccggtataa cgcttaaagc ataaaatgca gaggcataaa ttgggtacgc cgccgtagca
     7921 ccatcgagtc gcggccagtt ttgcgtaaac tgaatttgcg gtttaccacg cagtggtgtc
     7981 agttgattat tgagtttatc gggtcgccag gcccaggtat caatattttc gcgcacaccg
     8041 acaccgggat tgagctgttt atataaataa ccctgataac cagcgacaaa accaagcatg
     8101 agaaggataa acaggaggga attacggcaa cgtagcccgg atgtaccggt taaaggatgt
     8161 ctggttggcc ggccgtaccc cagcgaaaaa ctgatatgcg cgccaacggg tataatgact
     8221 atccaccagt actcacctcc gactactgag actaacaaaa cgcccagcca ggggagcata
     8281 agatcagcca accaacccga tcccatctgg tgaaattgat aatctgccag atgcagcgcg
     8341 attatccagc caaccaggat taataaaccg ggtactaaaa aaagacattg tcgccagacg
     8401 gctatttctc ttttttctgg tgcactatcg cctgcccata ctcgccccga gaaaaatagc
     8461 attaatgcgc ccagcgccag cataaaaatg ttgagcagaa ggtaacttgt tggcgtgata
     8521 aggatattac aaacttcaat aataccggca agtccgacac ccagcatagc aacaaccgcc
     8581 gccaaaaatt gcgtcagtat ggggatgccg aaaaaagtca ttaccagcga ggacaaaatc
     8641 cattttctgt tttgcattat tctttccatt ctttttgaat ggtgaaatta tactccccga
     8701 gtccccttgc cccttctgga cacttttccg aaatgatggc ggaaaaaaac gggacccttt
     8761 gg
//
LOCUS       DAJTVJ010000161         8634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000161 DAJTVJ010000000
VERSION     DAJTVJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.161"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="OO448_003768"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="OO448_003768"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6338868.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="OO448_003769"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="OO448_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6338869.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="OO448_003770"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="OO448_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6338870.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO448_003771"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO448_003771"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6338871.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO448_003772"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO448_003772"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6338872.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="OO448_003773"
     CDS             complement(7826..8137)
                     /locus_tag="OO448_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6338873.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO448_003774"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO448_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6338874.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2248 c         2051 g         2179 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaa
//
LOCUS       DAJTVJ010000162         8449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000162 DAJTVJ010000000
VERSION     DAJTVJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.162"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..450)
                     /locus_tag="OO448_003775"
     CDS             complement(<1..450)
                     /locus_tag="OO448_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6338875.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
     gene            complement(567..1613)
                     /gene="ansB"
                     /locus_tag="OO448_003776"
     CDS             complement(567..1613)
                     /gene="ansB"
                     /locus_tag="OO448_003776"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6338876.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1789..2508)
                     /locus_tag="OO448_003777"
     CDS             complement(1789..2508)
                     /locus_tag="OO448_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6338877.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2692..3018)
                     /locus_tag="OO448_003778"
     CDS             complement(2692..3018)
                     /locus_tag="OO448_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6338878.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="OO448_003779"
     CDS             complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="OO448_003779"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6338879.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3898..4950
                     /gene="mutY"
                     /locus_tag="OO448_003780"
     CDS             3898..4950
                     /gene="mutY"
                     /locus_tag="OO448_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6338880.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4978..5253
                     /locus_tag="OO448_003781"
     CDS             4978..5253
                     /locus_tag="OO448_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6338881.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5318..6397
                     /gene="mltC"
                     /locus_tag="OO448_003782"
     CDS             5318..6397
                     /gene="mltC"
                     /locus_tag="OO448_003782"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6338882.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6599..7855
                     /gene="nupG"
                     /locus_tag="OO448_003783"
     CDS             6599..7855
                     /gene="nupG"
                     /locus_tag="OO448_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6338883.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7904..8161)
                     /locus_tag="OO448_003784"
                     /pseudo
     CDS             complement(7904..8161)
                     /locus_tag="OO448_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            8289..>8449
                     /locus_tag="OO448_003785"
     CDS             8289..>8449
                     /locus_tag="OO448_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6338884.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSS"
BASE COUNT         2006 a         2224 c         2054 g         2165 t
ORIGIN      
        1 ataattgaaa tcttcgctat tcacgatcag atatttatta ttcgttttga tatcgtcgac
       61 cacccgccca cgatcgctgg ctgccgcctg aaaagagtaa aagttcaccg accagccaga
      121 cgcgggcgtt cctttactgg ttaacatcgc cgagaatttt accggtttat ctttcgtcca
      181 ggtctgttcg agcagttcgt aatctgccaa ctgaccgacc caggtataga gatcgtcact
      241 ggaagagaca tcgccttccg ctgaccaggt ggcctgatta cccttagcat caagattttt
      301 taaggtgata gcatcaagct tgagaatgcc gtgatactgc tttttgaatt gatcttttaa
      361 gatattttca gttggcggtt cgccatctgc ccaggcatta ccagtcatca acataagcag
      421 tgccgtgcca acaatccatt gctttttcac ccgaaaaacc tcacgcggaa taatttattc
      481 cggtgattat cattagggta atgaaaattg gcaatggacg taagtctgaa gtgaaaaagc
      541 cccggcacga taccggggcg aggtgattag tactgattga agatctgctg gatctgctgc
      601 ggatctttgg tttgcgtcag agccagctgc agcaggacgc gcgctttttg cgggttcagc
      661 gtgccagagg cgacgaagcc gtatttcgca tcatccactt cagcatcctg agtggtagca
      721 cccgtcggta cgcgggaaga acgcactact gcagtgccgt ttttcgcggc ggttgccagg
      781 gtgtcgaaca cggatttata caggttacca ttacccacac cagcgctaac gatgccatca
      841 tagcccgcat ctaccagtgc tttagccgga agatcggatg cgttagcgta gttataaacg
      901 atgccgactt tcggcagctc attcagctta gagacatcga atggcgtatc gctggtgtgc
      961 ttacgtgccg gggtacgttg gtagtcaatc ttaccgttgt gaatgtatcc cagaggaccg
     1021 tagttaacag acttgaaggt cgctacgtcg gtggtgttgg ttttggtgac atcgcgaccg
     1081 tccagtacgg tgtcgttcat caccaccagc acgccacgat tagcggaggc tttatcagct
     1141 gcggtcacta ccgcgttata caggttgaat ggaccgtctg cgctcatgga cgtggacggg
     1201 cgcattgcgc cgaccatcac caccggtttg tcgcatttca ccgtcaggtc gaggaagtaa
     1261 gcggtttctt ccatcgtgtc ggtaccgtgg gtaatgacga agccgtcggt tttatcgcag
     1321 tcggtgttaa tttttttcgc cagtgtcagc cagacattat cgttcatgtc ctgggagccg
     1381 atattcacta cctgctcgcc tttaacgttc gcaatgtctt ttagttgcgg caccgcatta
     1441 accagatttt ctacgccaac tttacccgct gtgtagttag atttggttgc ggagtcacca
     1501 ccaccggcaa tggtcccgcc ggttgctaaa atggtgatat tgggtaatgc caatgctgca
     1561 ccactaaaac ccataaccag tgcggcaagt gccgtctttt tgaaaaactc catttcattc
     1621 ctccagttac gtgaacgcta cgcattatcc cttagctctg tatgggaaat ttgacgttaa
     1681 acaatttaca acgtgaatat attttggaga tctacaaagt tagaggcagg taacaaaacg
     1741 aagaattaaa cggcataaaa aagtattatg ccgtcttaaa atagaggatt attttaaatt
     1801 cccgaccagg gctttgcggc tatcttccag agtcacaacg cggctacaaa catctttgcc
     1861 aaactgctgg aaatcttttt cctgcttttt ccactctgtt tggattgagg attgcagccc
     1921 ccccaggctt cccagcacat tctgtaatgg gttaccgccg cttttcagca ccgctttcgc
     1981 gcccatttca ttaatgctgt cctgtaaaat tccacccatt gcctgattca ctaattgctg
     2041 gccttcggcg cgaacctgat caatggcttt atagtgaaac gtcaggccat cgctgcgcgt
     2101 ttcgataatg cggttcatct gctctttcag ctgcgcatca agtttggtca gacggctgcg
     2161 cattttgctg ctttcgccca tctcctgaac gataatttta tccagcgcaa tacgggcttt
     2221 ttcgacgcgg cttttcgcgc cttcatcaat ccacggcagc gtgctgcgta gttcagcctg
     2281 ataatccttc gcctgctcgc gctgggcggc attcagggaa tattgcttac cgttatacat
     2341 cacgttgccg tctggcgtga tcaccagatt gccgttttcg cccttcacct gcacggtttg
     2401 cgggctgaca atcacatcgt cacgcggcgt gacgctgcac tggtagtcgg cgtgagcggt
     2461 cattgccgtc actgaaagtg ctgccgccag cagcattttg cgcatcatag tcttccctca
     2521 agaaaaaatc aggccagcat ttgctggccc cagattgata acaaagtacg cgttttccat
     2581 gccggatgcg gcgtaaacac cttatcccgc ctactcgatc gtgcatcttt ccaggcctga
     2641 taagcgtagc gcatcaggca gttttgcatt tgtcatcgcc cgtatgcttt cttagtccca
     2701 ccaaacgtcg aaaagttcgc tggtgcgtac ctcatccagt ttgcgctctt ccagccactt
     2761 acgcacaatc gcctgatgtt cttcggtgca tttgccgatt tcctgcatgc agatcagacc
     2821 ttcccaggcc agataaccgc tgccgtcaaa ggccagtttg ttcggttcga taacctcgtt
     2881 aataaaatca tcaacggttt tatcaatctg ttcttccgat gtaccttccg ggaatcgcca
     2941 tgccaccgaa aatcctaatt cctggaattc gtcgatgtgc atttttttac gcagacgacg
     3001 gctacggttc tttgccatta tttcaccctc tcgaacatta agtcccatac tccgtgacca
     3061 agacgatgac cacgttgttc aaatttcgtc accggacgtg atgccggacg cggcacgtaa
     3121 tcattgctct ctgacaggtt tttataaccg tcaatagaag acatcacttc aagcatatgt
     3181 tccgcataag gttcccagtc ggtcgccata tggaatacac cccccagctg cagtttgctt
     3241 tttaccagtt cggcaaacgg cacctgtacg atacggcgtt tattatggcg cgctttgtgc
     3301 cacgggtcag ggaaaaagag ctgcaccatg cgcaatgaat tgtcaggaat cattttatgc
     3361 agcacttcaa ccgcatcgtg acacatcacg cgcaggttgc ttaaaccttc ttcatgcgca
     3421 gaagccaggc acgcaccaac gcccggtgaa tgcacttcaa tgccgaggaa gtcctgctca
     3481 gggcgatctt tagccattgc caccagcgac gcccccatgc caaaaccaat ctcaagcgtc
     3541 accggcgctt cacggccaaa aagcgcgggg aaatccagca tatcttcgct gaactcaacg
     3601 cccatcaccg gccagtagtt ttccagcgca tgttcctggc ctttggtcag tcgcccctgg
     3661 cggcgcacaa aactacggat acggcgcagt gggcggccgt tttcatcaaa ttccggtgaa
     3721 atgacgtcgt ttttcataaa gatttagtcg cttgtgaaag tgttctgaaa acgggcatta
     3781 tccaaagtta gttgccggat gcaagcatga taaggccgtg gctgcggaaa gttccggttt
     3841 acaccctgcc gtcgctgtgc tgcaatcttg cccccaacaa cagtgaattc ggtgaccatg
     3901 caagcgtcgc aattttcagc ccaggttctg gactggtacg ataaatacgg gcgaaaaacg
     3961 ctgccctggc aaattgacaa gacgccctac aaagtatggc tctcagaagt gatgttgcaa
     4021 caaactcagg ttgcgaccgt tatcccctat tttgaacgct ttatggcgcg cttcccgacg
     4081 gtgaccgatc tcgccaatgc gccgctcgac gaagttctcc acttgtggac cgggcttggc
     4141 tattacgccc gcgcgcgcaa tctgcataaa gcggcacaac aagtggcgac cttacacggc
     4201 ggtaaattcc cggaaacctt tgaagaagtc gcggcgttac cgggcgtcgg gcgttccacc
     4261 gcaggcgcga ttctctcgct ttctctgggt aagcactttc cgattctcga cggtaacgtc
     4321 aaacgcgtgc tggcgcgctg ctatgctgta agcggctggc ctgggaaaaa agaggtcgag
     4381 aataaattat ggagtttgag cgagcaggtg acgcccgcgg tcggcgtgga acggtttaat
     4441 caggcgatga tggatttggg cgcgatgatt tgtacgcgct cgaagccgaa atgttcgctc
     4501 tgtccgctac aaaacggatg tattgccgcc gccaataata gctggtcgct ttatccgggc
     4561 aaaaaaacga aacagacgct gccggagcgc accggctact ttttgctgtt acagcacgaa
     4621 gatgaagtat tgctggcgca gcgtccgccg agcggattgt ggggcggttt atactgtttc
     4681 ccgcagtttg ccgacgaaga aagtttgcgg cagtggctgg cgcaacggca gattgctgcc
     4741 gataacctga cgcaactgac cgcgtttcgg cataccttca gccatttcca cttagatatt
     4801 gtgcctatgt ggcttcccgt gtcgtcattc accggctgca tggatgaagg caatgcgctc
     4861 tggtataact tagcgcaacc gccgtcagtt ggcctggcgg ctcccgtgga gcgtttgtta
     4921 cagcagttac gcactggcgc gccggtttag cgcgtgagtc gataaagagg atgatttatg
     4981 agcagaacga ttttttgtac tttcctgcaa cgtgaagcag aaggtcagga ttttcagctg
     5041 taccccggcg agctgggaaa acgcatctat aacgagatct ccaaagaagc ctgggcgcag
     5101 tggcagcaca agcaaaccat gctgattaat gaaaagaaac tcaacatgat gaatgccgag
     5161 caccgcaagc tgcttgagca ggagatggtc aacttcctgt tcgagggtaa agaggtgcat
     5221 atcgagggct atacgccgga agataaaaaa taaaaacagt gccggagcac gcctccggca
     5281 acttgcataa aaacaaacac aacacgcacc cggaatgatg aaaaaatatc tcgcgctggc
     5341 tttgattgcg ccgttgctca tctcctgttc gacgaccaaa aaaggcgata cctataacga
     5401 agcctgggtc aaagatacca acggttttga tattctgatg gggcaatttg cccacaatat
     5461 tgagaacatc tggggattca aagaggtggt gatcgccggt cctaaggact acgtgaaata
     5521 caccgatcaa tatcagaccc gcagccacat caacttcgat gacggtacga ttactatcga
     5581 aactatcgcc gggacagaac ctgccgcgca tttgcgccgg gcaattatca aaacgttgct
     5641 gatgggtgac gatccgagtt cggtcgatct ctattccgac gttgatgata ttacgatttc
     5701 gaaagaacct ttcctttacg gtcaggtggt ggacaacacc gggcagccga ttcgctggga
     5761 aggtcgcgca agcaacttcg cggattatct gctgaaaaac cgtctgcaaa gccgcagtaa
     5821 cgggctgcgt atcatctaca gcgtcaccat taacatggta ccgaaccacc ttgataaacg
     5881 tgcgcacaaa tatctcggca tggtccgcca ggcgtcacgg aaatatggcg ttgatgagtc
     5941 gctgattctg gcaattatgc agaccgaatc ttcctttaac ccgtatgcgg tcagccgttc
     6001 cgatgcgctg ggattaatgc aggtggtaca acatactgcc gggaaagatg tgttccgctc
     6061 gcaggggaaa tccggcacgc cgagccgcag tttcttgttt gatcctgcca gcaatattga
     6121 taccggcacc gcgtatctgg cgatgctgaa caatgtttat ctcgacggaa ttgataaccc
     6181 aacatcgcgg cgttatgccg tcatcaccgc ctataacggc ggcgcaggca gcgtgctgcg
     6241 agtcttttcg aatgataaga ttcaggctgc caatattatt aacaccatga cgccgggcga
     6301 tgtttatcag acgctgacga cccgccatcc ctctgcggaa tctcgccgtt atctttataa
     6361 agtgaatacc gcgcaaaaat cctaccgccg ccgataattc cattaaccgc ccctgacgat
     6421 gctcaggggc aaaaatgtta tccacatcac aatttcgttt tgcaaattgg gaatgtttgc
     6481 aattatttgc cacaggtaac aaaaaaccag tccgcgaagt tgatagaatc ccatcatctc
     6541 gcacggtcaa atgtgctttt tcaaacactc atccgcatca caatgtgagg aaattaacat
     6601 gaatcttaag ctgcagctga aaatcctctc ttttctgcag ttctgtctgt ggggaagttg
     6661 gctgacgacc ctcggctcct atatgtttgt taccctgaag tttgacggtg cttctattgg
     6721 cgcagtttat agctcactgg gtatcgccgc ggtatttatg cctgcgctgc tggggattgt
     6781 ggccgacaaa tggttaagtg cgaaatgggt atatgccatt tgccacacca ttggcgctat
     6841 cacgctgttc atggcggcac aggtcacgac gccggaagcg atgttccttg tgatattgat
     6901 taactcgttt gcttatatgc caacgcttgg gttaatcaac accatctctt actatcgcct
     6961 gcaaaatgcc gggatggata tcgttactga cttcccgcca atccgtatct ggggcaccat
     7021 cggctttatc atggcaatgt gggtggtgag cctgtctggc ttcgaattaa gccacatgca
     7081 gctgtatatt ggcgcagcac tttccgccat tctggttctg tttaccctga ctctgccgca
     7141 tattccggtt gctaaacagc aagcgaatca gagctggaca accctgctgg gcctcgatgc
     7201 attcgcgctg tttaaaaaca agcgtatggc aatcttcttc atcttctcaa tgctgctggg
     7261 cgcggaactg cagattacca acatgttcgg taataccttc ctgcacagct tcgacaaaga
     7321 tccgatgttt gccagcagct tcatcgtgca gcatgcgtca atcatcatgt cgatttcgca
     7381 gatctctgaa accctgttca ttctgaccat cccgttcttc ttaagccgct acggtattaa
     7441 gaacgtaatg atgatcagta ttgtggcgtg gatcctgcgt tttgcgctgt ttgcttacgg
     7501 cgacccgact ccgttcggta ctgtactgct ggtactgtcg atgatcgttt acggttgcgc
     7561 attcgacttc ttcaacatct ctggttcggt gtttgtcgaa aaagaagtta gcccggcaat
     7621 tcgcgccagt gcacaaggga tgttcctgat gatgactaac ggcttcggct gtatcctcgg
     7681 cggcatcgtg agcggtaaag ttgttgagat gtacacccaa aacggcatta ccgactggca
     7741 gaccgtatgg ttgattttcg ctggttattc cgtggttctg gccttcgcgt tcatggcgat
     7801 gttcaaatat aaacacgttc gtgtcccgac aggcacacag acggttagcc actaattacg
     7861 caaagaaaaa cgggtcgcca gaaggtgacc cgttttttta ttcttacttc aacacataac
     7921 cgtacaatcg tttcatgcca tccgcatcgg tttcgctata gacaccttgc aactccggcg
     7981 aaaatcccgg caacaaattc acgccttctt ccagcgcaag gaaataacgt tgaaccgccc
     8041 caccccagac ttccccggga accacgcaaa gcacgccagg tggataaggc aacgcccctt
     8101 ctgccgcaat tcgcccttcg gcatcacgaa tccgcaccaa ctccacttca ccgcgaatat
     8161 aaggcagtat gactttccgt gagacagatg ctggccaacg tgggatattt aacgcgggtt
     8221 aacatcgttg tcagagccgc atgggtgagc tgttgctaca ccccggtatt tccaccacgg
     8281 aggccagcat gaacaacaat aacacattgt acgtcgggtt agatgttcac aaagaatcga
     8341 ttactgtcgc ttatgctatc aattcagaac ctgttgaact gatgggtaaa attggcacat
     8401 cacctactga tattcagaat ctttgtaaac gtctcaggtc aaagtcatc
//
LOCUS       DAJTVJ010000163         8389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000163 DAJTVJ010000000
VERSION     DAJTVJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.163"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            339..860
                     /gene="fecI"
                     /locus_tag="OO448_003786"
     CDS             339..860
                     /gene="fecI"
                     /locus_tag="OO448_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR6338885.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            857..1810
                     /gene="fecR"
                     /locus_tag="OO448_003787"
     CDS             857..1810
                     /gene="fecR"
                     /locus_tag="OO448_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR6338886.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1897..4221
                     /gene="fecA"
                     /locus_tag="OO448_003788"
     CDS             1897..4221
                     /gene="fecA"
                     /locus_tag="OO448_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR6338887.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4266..5168
                     /gene="fecB"
                     /locus_tag="OO448_003789"
     CDS             4266..5168
                     /gene="fecB"
                     /locus_tag="OO448_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR6338888.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5165..6163
                     /gene="fecC"
                     /locus_tag="OO448_003790"
     CDS             5165..6163
                     /gene="fecC"
                     /locus_tag="OO448_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR6338889.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLCLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6160..7116
                     /gene="fecD"
                     /locus_tag="OO448_003791"
     CDS             6160..7116
                     /gene="fecD"
                     /locus_tag="OO448_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR6338890.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7117..7884
                     /gene="fecE"
                     /locus_tag="OO448_003792"
     CDS             7117..7884
                     /gene="fecE"
                     /locus_tag="OO448_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR6338891.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1839 a         2399 c         2333 g         1818 t
ORIGIN      
        1 ccttcagtag cggagcagga aggacatctg atggaaatgg aagccacgca agcaccttaa
       61 aatcaccatc atacactaaa tcagtaagtt gacagcatta ccgaaatccc tatcctttag
      121 ggcagtgagg atgtcaataa tagcagacca catcatgagt cattctgacc gacacatgcc
      181 agaagcagag gctccatccc attttatacc taccttataa cacttagaaa aacaacatgt
      241 taaaaatgtc tattggaaac aattttattt ccaattgtaa tgataactat tctcatatta
      301 atatggctac gtgataatta acttttgatg cactccgcat gtctgaccgc gccactacca
      361 cagcttcctt aacattcgag tcgctttatg gcacacatca cggctggttg aaaagctggc
      421 tgacgcgcaa actccagtct gcttttgatg cagatgacat tgcccaggac acttttttgc
      481 gggtaatggt cagcgaaacg ctctcgacga tccgcgatcc tcgctccttc ctctgcacta
      541 tcgccaaacg cgtgatggtg gacctgtttc gccgaaacgc gctggaaaaa gcgtatctgg
      601 agatgctggc gcttatgccg gaggggggag cgccttcacc tgaggaacgc gaaagccaac
      661 tcgagaccct acaactcctc gacagcatgc tggacgggct aaacggcaaa acacgtgaag
      721 cgtttctgct ttcgcaactg gatggtctga catacagcga gattgcgcac aaactcggtg
      781 tttccatcag ctccgtgaaa aaatacgtgg cgaaagccgt cgagcactgc ctgctgttcc
      841 gtctggagta tgggttatga atcctttgtt aaccgattcc cgccgtcagg cgctgcgttc
      901 agcttcccac tggtatgccg tgctaagcgg cgagcgcgtc agcccacaac aggaagcgcg
      961 ctggcaacag tggtatgaac aggatcagga taaccagtgg gcctggcagc aggttgaaaa
     1021 cctgcgcaac cagcttggcg gtgtgcctgg cgacgttgcc agccgggcgt tgcacgatac
     1081 ccgcctcacc cgccgtcacg tgatgaaagg attactgctg ttgctcggcg ctggcggagg
     1141 ctggcagctc tggcagtcgg aaaccggcga aggtctgcgg gcagattacc gcaccgccaa
     1201 aggcacagtc agccgtcagc aactggaaga tggctccctg ctcacgctga atacccaaag
     1261 cgcggcggat atgcgttttg atgcgcatca gcgcaccgtc cggctctggt acggtgaaat
     1321 cgccattact accgcgaaag atgccctgca acgccccttc cgtgtcctga cccgtcaggg
     1381 ccagctcact gctttaggga cagaatttac cgtccgccag caggataatt tcacgcagct
     1441 tgacgtgcag cagcacgccg tggaagtgct tctcgccagt gcccccgcgc aaaaacgcat
     1501 cgtgaacgct ggtgaaagcc tgcagttcag cgcctctgag tttggcgcag tgaaaccgct
     1561 ggatgacgag agtacaagct ggacgaagga catcctgagc ttcagcgata aaccgctggg
     1621 tgaggtgata gccacgctaa cccgttaccg caacggcgtg ctgcgctgcg atcccgccgt
     1681 tgccgggctg cgcctgagcg ggacgttccc gctgaaaaat accgatgcga tcctgaacgt
     1741 tatcgcgcaa acgcttcccg ttaaaattca gtctattacg cggtactgga taaacatttc
     1801 accactgtaa ggaaaataat tcttatttcg attgtccttt ttacccttct cgttcgactc
     1861 atagctgaac acaacaaaaa tgatgatggg gaaggtatga cgccgttacg cgtttttcgt
     1921 aaaacaacac ctttggttaa cgccattcgc ctgagcctgc tgccgctggc cggtctctcg
     1981 ttttccgctt ttgctgcaca ggttaatatc gcaccgggat cgctcgataa agcgctcaat
     2041 cagtatgccg cacacagcgg atttaccctc tcggttgacg ccagcctgac gcgcggcaag
     2101 cagagcaacg gcctgcacgg cgattacgac gtcgagagcg gcctgcaaca actgctggac
     2161 ggcagcggac tgcaggtaaa accgctggga aataacagct ggacgctgga gcccgcgccc
     2221 gcaccaaaag aagatgccct gaccgtggtc ggcgactggc tgggtgatgc gcgtgaaaac
     2281 gacgtatttg aacatgctgg cgcgcgtgac gtgatccgcc gtgaggattt cgccaaaacc
     2341 ggcgcaacca ccatgcgtga ggtacttaac cgcatccctg gcgtcagcgc gccggaaaac
     2401 aacggcaccg gcagccacga cctggcgatg aactttggca tccggggcct gaacccgcgc
     2461 ctcgccagcc gctcgaccgt cctgatggac ggcatccccg tccccttcgc cccttacggt
     2521 cagccgcagc tttcactggc tcccgtttcg ctcggcaaca tgaatgccat tgacgtggta
     2581 cgcggtggtg gtgcggtgcg ttacggaccg cagagcgtgg gcggcgtggt gaactttgtt
     2641 acccgtgcca ttccgcagga ctttggtatc gaggcgggcg tggaaggtca gctcagccca
     2701 acctcttcac aaaacaaccc gaaagagacg cacaacctga tggtgggcgg cacagcggac
     2761 aacggttttg gcaccgcgct gctctactcc ggcacgcgcg gcagtgactg gcgcgagcac
     2821 agcgccaccc gcatcgacga cctgatgctg aaaagcaaat atgcgccgga tgaggtgcac
     2881 accttcaaca gcctgctgca atattacgac ggtgaagccg acatgcccgg tggcctgtct
     2941 cgcgcggatt acgacgccga tcgctggcaa tccacccgcc cgtatgaccg cttctggggt
     3001 cgtcgcaagc tggcgagcct gggctaccag ttccagccag acagccagca taaattcaac
     3061 attcaggggt tctacaccca aaccctgcgc agcggctacc tggagcaagg caaacgcatc
     3121 accctctcgc cgcgtaacta ctgggtgcgc ggtattgagc cacgctacag ccagatcttt
     3181 atgatcggcc cttccgcgca cgaagtgggc gtgggctatc gctatgtgaa tgaatcaacg
     3241 catgaaatgc gttactacac cgccaccagc agcgggcagt tgccgtccgg ctcaagccct
     3301 tacgaccgcg atacgcgttc cggcaccgag gcgcacgcct ggtatctgga tgacaaaatc
     3361 gacatcggca actggaccat cacgccgggt atgcgtttcg aacatatcga gtcataccag
     3421 aacaacgcca tcacaggcac gcacgaagaa gtgagctata acgcaccgct tccggcgttg
     3481 aacgtgctct atcacctgac tgacagctgg aatctttatg caaacactga aggctcgttc
     3541 ggcaccgtac agtacagcca gattggcaag gctgtgcaaa gcggcaatgt ggaaccggaa
     3601 aaagcgcgaa cctgggaact cggtacccgc tacgacgacg gcgcgctgac ggcggaaatg
     3661 gggctgttcc tgattaactt taacaatcag tacgactcca accagaccaa cgacaccgtc
     3721 actgcacgtg gcaaaacgcg ccataccggg ctggaaacgc aggcacgtta cgatctgggt
     3781 acgctaacgc caacgcttga taacgtttcc atctacgcca gctatgcgta tgtgaacgcg
     3841 gaaatccgcg agaaaggcga cacctacggc aatctggtac cattctcccc gaaacataaa
     3901 ggcacgctgg gcgtggacta caagccagga aactggatgt tcaatctgaa cagcgatttc
     3961 cagtccagcc agtttgcgga taacgccaat acggtgaaag agagcgccga cggcagtacc
     4021 ggccgcattc ccggcttcat gctctggggc gcacgcgtgg tgtatgactt tggcccgcag
     4081 atggcagatc tgaacctggc attcggtgtg aaaaacatct tcgaccagga ctacttcatc
     4141 cgctcttatg acgacaacaa caaaggcatc tatgcaggcc agccgcgcac gctgtatatg
     4201 cagggagcgt tgaagttctg atgtgttaac gcccggcttg ccgggctttt agctggaatg
     4261 tgattatgtt ggcatttatc cgttttcttt ttgcaggcct gctgctggtg atcagccacg
     4321 cctttgccgc cacggttcag gacgaacacg gcacgtttac actcgaaaaa acgccacaac
     4381 ggattgtggt gctggaactc tcgttcgccg atgcgctggc cgccgtggac gtcagcccga
     4441 tcggtattgc cgacgataac gatgcaaaac gcatcctgcc cgaagtgcgt gcgcacctga
     4501 aaccgtggca gtccgtcgga acgcgcgcgc agccgagcct ggaagccatt gccgctctga
     4561 aaccagacct gatcattgcc gacagcagtc gccatgcggg ggtttacatc gccttgcagc
     4621 aaatcgcgtc ggtactgctg cttaagtccc gcaacgaaac ctacgctgaa aatttgcaat
     4681 ctgcggctat catcggcgaa atggtgggta aaaagcgaga gatgcaggca cgtctggaac
     4741 aacataaaga gaggatggcg cagtgggcca gccagcttcc caaagggaca cgcgtggcct
     4801 ttggcacatc acgcgaacag caattcaacc tgcatactca ggagacctgg accggcagcg
     4861 tgctggcctc tctggggctg aacgttcccg ctgcgatggc gggtgcgtcc atgccgtcca
     4921 tcggcctgga gcaactgctg gcggtcaatc ctgcctggct gctggttgcc cactatcgcg
     4981 aagagagcat tgttaaacgc tggcaacaag atccgctctg gcagatgtta accgccgcgc
     5041 agaagcagca ggttgcttcg gtcgacagta acacctgggc gcggatgcgc ggtatttttg
     5101 ctgcagagcg tattgccgct gacacggtaa aaatcttcca ccatcagccg cttaccgttg
     5161 tgaaatgacc gcgataaaac acccggtgct gctgtggggg cttcccgttg cagcacttat
     5221 tattattttc tggctgagtc tgttttgcta ctcggccatt cctgtttccg gagcagatgc
     5281 aacccgcgcc ctgctgcctg gacacacgcc aacgctacca gaagcgctgg tgcaaaacct
     5341 tcgtttgcca cgaagcctgg tcgccgttct gatcggcgca agcctggcgc tcgcgggcac
     5401 gctgctgcaa accctgaccc acaacccaat ggcctctcct tcactgctcg gcattaacag
     5461 cggcgcggcg ctggctatgg cgcttaccag cgcgctgagt ccgacgccga ttgcaggcta
     5521 ttctctgtcg ttcatcgcgg catgcggggg cggcgtgagc tggctgctgg tcatgaccgc
     5581 aggaggcgga tttcgtcata cccatgacag aaacaaactg atcctcgcgg gtatcgcgct
     5641 gtcggccttt tgtatgggcc ttacccgcat caccctgctg ctggccgaag atcatgctta
     5701 cggcatcttt tactggctgg ctggcggagt gtcccacgcc cgctggcagg atgtctggca
     5761 gctcttgccg gtggtggtca ctgcagtccc tgtcgtgttg ctgctggcga atcaactgaa
     5821 cctgctcaac ctcagcgaca gcaccgccca tacgctggga gtgaacctga cgaggctatg
     5881 tttggtcatc aatatgttag tgctgcttct ggttggtgcg tgcgtcagcg tggcaggtcc
     5941 ggtggcgttt atcggtctgc tggtgccaca tctggcgcgc ttctgggcag gcttcgatca
     6001 gcgcaacgta ctgccagtga gcatgctgct gggggccacg ctgatgctgc tggcagatgt
     6061 actcgcacgc gcgctggcct tccccggaga tctgcccgca ggcgcagtgc tggcgctgat
     6121 tggcagccct tgctttgtct ggcttgtgag gaggcgagga tgaaaattgc gctggttatt
     6181 ttcatcaccc ttgccctggc gggctgtgcg ctgttatcac tccatatggg agtgatcccc
     6241 gtgccgtggc gcgcgctgct gaccgactgg caggccggac gcgagcatta ttatgtattg
     6301 atggagtacc gactgccgcg cttgctgctg gcactgtttg tcggtgcagc cctcgccgtg
     6361 gcgggcgtgc tgatacaggg gattgtgcgc aaccctctgg catcaccgga tattctcggc
     6421 gttaaccatg ccgccagcct ggcctctgtg ggggctctac ttcttatgcc gtcactgccc
     6481 gtgatggtgc tgccgctgct ggcctttgcg ggcggcatgg cggggttgat attgctgaag
     6541 atgctggcaa agacccacca gccgatgaag ctggcactca ccggcgtggc gctttctgca
     6601 tgctgggcca gcctgacgga ttatctgatg ctctcgcgcc cgcaggatgt gaacaacgcc
     6661 ctgctgtggc tgaccggcag cttatggggc cgtgactgga gctttgtgaa gattgccatc
     6721 ccgctgatga ttttatttct gccgctgagc ctgagttttt gccgcgatct cgacctcctt
     6781 gcactcggcg atgcgcgcgc caccacgctc ggtgtgtcgg tgccctatac ccgattctgg
     6841 gctttgttac tagctgtcgc catgacatct accggcgtgg ccgcctgcgg cccgattagc
     6901 tttattggtc tcgtggtgcc gcatatgatg cgtagcatca ccggtggacg tcaccgcaga
     6961 ctgctgcctg tttcggccct gacaggtgcg ttgctgttgg tggttgccga tctgctggcg
     7021 agaattattc atcccccact ggagctcccg gttggcgtgc tgaccgccat tatcggtgcg
     7081 ccgtggtttg tctggttgct tgtgagaatg cgataaatga ctttacgaac tgaaaatctg
     7141 acggtcaatt acggaacaga caaggtactt aacgacgttt cactctcact gccaacgggg
     7201 aagatcaccg ccctgatcgg tcctaacggt tgcgggaaat cgacgctgtt aaactgtttt
     7261 tcgcggcttt taatgccgca gtctggcacc gtatttctcg gcgataatcc cataaatatg
     7321 ctctcatcgc gccagttggc ccgcaggctt tcgctgctgc ctcagcacca tttaacgcca
     7381 gaggggatca cagtccagga gctggtttcg tatggtcgta atccctggct gtcactctgg
     7441 gggcgtctct ccgctgaaga caatgcacga gttaatgtcg ccatgaacca gacccggatc
     7501 aatcatcttg ccgttcgtcg gttaaccgag ctttccggcg gtcagcgcca gcgcgcattt
     7561 ctggcaatgg tcctggccca gaatacgccc gttgtattac ttgatgagcc aaccacctat
     7621 cttgatatca atcaccaggt ggacctgatg cggttgatgg gcgaactccg gactcagggg
     7681 aaaacggtgg tcgctgtgct gcacgacctt aatcaggcta gccggtactg cgatcaactg
     7741 gtggtaatgg caaacggaca tgttatggcg caaggcacac cagaagaggt gatgacccca
     7801 ggattgctga gaacagtatt cagcgtggaa gcggaaatac accccgagcc ggtatctggc
     7861 aggccgatgt gcctaatgag gtagattgca caggccgtaa gaaccaaacc acgactgaat
     7921 gaaactggac tggcgccagc aagcctgttc agactggggc tgaacttttc cggactctga
     7981 aagattacca atactcatcg tccatccgct tgctttaggc tgacaggttc ataatcaacg
     8041 caaaccagag ctgtacaggc ttgggcgcgg ctttcaaacc agtcgtgatc acggcaatca
     8101 attttgaact ctgcttaacg gacatttctg tataaccctt acggcaacga aaaacgcgaa
     8161 gttaaaattt tagaaaccca aaaacgtgac atgactaagt ttagatttca ggggggggag
     8221 atcaaaaaat ttcgctctgt gccagagcgg acattcacgg agctggttca ttaccaatga
     8281 ggttgggctt ttgaggataa atcaatgatc agacgccaac gtaaatcaaa agcaaccctg
     8341 gaacggaatt gctaatccag tttctgacca tcgatttttc taaaaagtg
//
LOCUS       DAJTVJ010000164         8326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000164 DAJTVJ010000000
VERSION     DAJTVJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.164"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            267..1925
                     /locus_tag="OO448_003793"
     CDS             267..1925
                     /locus_tag="OO448_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6338892.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="OO448_003794"
     CDS             complement(1922..2878)
                     /locus_tag="OO448_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6338893.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="OO448_003795"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="OO448_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6338894.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="OO448_003796"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="OO448_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6338895.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO448_003797"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO448_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6338896.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6789..8027
                     /locus_tag="OO448_003798"
     CDS             6789..8027
                     /locus_tag="OO448_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6338897.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
BASE COUNT         2057 a         1960 c         2233 g         2076 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatttatc atctagtgtt cgcgggtgtt cgtggtagtg ttttgtgtgc
     6661 attcatcgct atgattataa aggaaaattt tgttttcctt gttcgctctt gttcgtcacg
     6721 gtttgccctt gtacgatgca cggtgtagtc aatggtgtag ttaattttga tgaccggaaa
     6781 cgcgaaacat gagcagcgca attcatagac ttagcgacac tcttttacga aaattaagcg
     6841 gatcaccaac cacaaaaaac gcctttttta atgacggtgg caatcttagc gtaagacatt
     6901 ccaccagcgg cctgttaacc tggtatttca cttacagggc cggaactggc aggcaggtat
     6961 caccggaacg cctgagactg ggcaactatc ccgatctgag cctgaaagca gccagggaaa
     7021 aagcggcaca gtgtcgcgcc tggctggctg aggggaaaaa tccgcgctat gagcttaatc
     7081 gtgctgtaca ggatgcgtta gcccccgtga cagttaagga agcgctcact tactggcttg
     7141 aatcgtatgc aaaggaaaag cgcactgact acgaatcact gaaaagccgg atcaataaac
     7201 acataatcag ccagattggc gctttgccgt tggaaaaatg cgagctacgg cactggttgg
     7261 catgttttga ccagatggca aagcgaagcc cagtatctgc cggatttttg ctacaggtat
     7321 gcaaacaggc gcttaagtac tgccgaaaac gacgatacgc aatcagcaac gttcttgatg
     7381 atatggttgt cggtgatgtg ggaaaaaaag cagaaataag cgagcgcgta ctaacaaaca
     7441 aggagcttgg ggaattactc cgcgccctgg atgaaaaaat ttacccaccg tactacagcg
     7501 ccctgattcg cctcctgatt gtcttcggat gccgtgccac tgagctaaga cgctctgagg
     7561 tccaggaatg ggattttaaa gaaatgctct ggaccgtgcc gaaagaacac agcaaaacga
     7621 aggtagcaat attcaggcca ataccggaag cgatcttgcc gttcgtaacg aagctggtgg
     7681 agcagaacag gcacacggga ttattgctcg gagaactgaa agggcaatca tccgtatcag
     7741 agtacggaag aacggcacac agacgcatta atcaagcccc ctggacgttg cacgacatcc
     7801 ggcacacgtt tacaacgatg ctgaacgatt taggcgtgga tcctcatgta gtggagcagc
     7861 taaccgcgca ccagttgcca ggagtgcaac gagtctataa tcactcccgt tatctcgatg
     7921 caaaacgtga cgctcttaat ctatgggttg agcgtctcga gcttctccag aacaatgatg
     7981 aaaaaatcgt tgttatgacc ccgcgaattt actcccaaaa ttcttgacaa attacggctg
     8041 tttttcttgc gaaaaatggc cctctaaaat aaatcgtcga atatcggcaa atatcgacga
     8101 tttatatgtg tcaactatcg aaaaaaattg aaatgtcttc ttctgagttc ttattctctg
     8161 tttcttgatt attccaatac ctgatggttt atctatttgt ttttaatgag gtaattcagg
     8221 tgtcatgcca aatggcgaca ttttttgttg cgtcgcttaa aaactcgtga cacgatttta
     8281 aacactaacg agcttctacg aaccttagcg aacaaagcct ttagca
//
LOCUS       DAJTVJ010000165         8274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000165 DAJTVJ010000000
VERSION     DAJTVJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.165"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..936
                     /locus_tag="OO448_003799"
     CDS             52..936
                     /locus_tag="OO448_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6338898.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO448_003800"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO448_003800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR6338899.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO448_003801"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO448_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6338900.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OO448_003802"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OO448_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6338901.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OO448_003803"
     CDS             5160..6443
                     /locus_tag="OO448_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR6338902.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="OO448_003804"
     CDS             6590..8065
                     /locus_tag="OO448_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6338903.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2193 a         1981 c         1914 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaaccgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggca
//
LOCUS       DAJTVJ010000166         8214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000166 DAJTVJ010000000
VERSION     DAJTVJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.166"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OO448_003805"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="OO448_003805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCR6338904.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OO448_003806"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="OO448_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6338905.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="OO448_003807"
     CDS             2028..2546
                     /locus_tag="OO448_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6338906.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OO448_003808"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="OO448_003808"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6338907.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="OO448_003809"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="OO448_003809"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6338908.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="OO448_003810"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="OO448_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6338909.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="OO448_003811"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="OO448_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6338910.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="OO448_003812"
     CDS             complement(6710..8170)
                     /locus_tag="OO448_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6338911.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2171 a         2055 c         1870 g         2118 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaaac tatatgtttt gtta
//
LOCUS       DAJTVJ010000167         8184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000167 DAJTVJ010000000
VERSION     DAJTVJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.167"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="OO448_003813"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="OO448_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR6338912.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="OO448_003814"
     CDS             complement(2975..3697)
                     /locus_tag="OO448_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6338913.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="OO448_003815"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="OO448_003815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6338914.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8184
                     /locus_tag="OO448_003816"
     CDS             6679..>8184
                     /locus_tag="OO448_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6338915.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNAA"
BASE COUNT         1980 a         2031 c         2321 g         1852 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cgca
//
LOCUS       DAJTVJ010000168         8113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000168 DAJTVJ010000000
VERSION     DAJTVJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.168"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            163..317
                     /locus_tag="OO448_003817"
                     /pseudo
     CDS             163..317
                     /locus_tag="OO448_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="OO448_003818"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="OO448_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCR6338916.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="OO448_003819"
     CDS             858..2264
                     /locus_tag="OO448_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR6338917.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /locus_tag="OO448_003820"
     CDS             2509..3654
                     /locus_tag="OO448_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6338918.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="OO448_003821"
     CDS             3672..4685
                     /locus_tag="OO448_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6338919.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="OO448_003822"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="OO448_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..6370
                     /locus_tag="OO448_003823"
     CDS             5576..6370
                     /locus_tag="OO448_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6338920.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6392..7816
                     /gene="patD"
                     /locus_tag="OO448_003824"
     CDS             6392..7816
                     /gene="patD"
                     /locus_tag="OO448_003824"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6338921.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            8026..>8113
                     /locus_tag="OO448_003825"
     CDS             8026..>8113
                     /locus_tag="OO448_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCR6338922.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCM"
BASE COUNT         1912 a         1908 c         2222 g         2071 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt
     6181 cgatgaaatc atcgttacga cctttacggc aggtcatgaa cgaacgttac tgttgtggtt
     6241 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
     6301 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
     6361 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
     6421 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
     6481 actggaaatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     6541 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     6601 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     6661 caaaccgctg catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt
     6721 tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca
     6781 tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta
     6841 tccgctgatg atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt
     6901 gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga
     6961 tatcttcccg gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc
     7021 gctgaccggt catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga
     7081 gcacatcatc agccatgccg cgtcgtccat taagcgtact catatggaac ttggtggcaa
     7141 agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac
     7201 atttggctat tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa
     7261 aggcatttac gatacgctgg tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg
     7321 tgcgccggat gacgagtcta cggagcttgg acctttaagc tcgctggcgc atctcgaacg
     7381 cgtcagcaag gcagtagaag aggcgaaagc gacagggcac atcaaagtga tcactggcgg
     7441 tgaaaagcgc aagggtaatg gctattacta tgcgccgacg ctgctggctg gcgcattaca
     7501 ggacgatgcc atcgtgcaaa aagaggtatt tggtccagta gtgagtgtta cgcccttcga
     7561 caacgaagaa caggtggtga actgggcgaa tgacagccag tacggacttg catcttcggt
     7621 atggacgaaa gatgtgggca gggcgcatcg cgtcagcgca cggctgcaat atggttgtac
     7681 ctgggtcaat acccatttca tgctggtaag tgaaatgccg cacggtgggc agaaactttc
     7741 tggttacggc aaggatatgt cactttatgg gctggaggat tacaccgtcg tccgccacgt
     7801 catggttaaa cattaagtta gatgttagtg gctgttatag gacggccact atcgttttag
     7861 gttctgagtt taatggcggt taagtattag aatgattaca ttaataacta gcgtaaaaat
     7921 taggctaaat gtttgatggc gatttttcat cctgtcacag ttgattagca taaggcttta
     7981 gagtatcgtt attaaactaa taatttataa gtagaagtat taacaatgaa agttcaattg
     8041 tacaaattca cggaagataa aaataaaacc cttaccttca gatggacgaa aaaacacttt
     8101 gagttttgta tgg
//
LOCUS       DAJTVJ010000169         8104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000169 DAJTVJ010000000
VERSION     DAJTVJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.169"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(101..796)
                     /locus_tag="OO448_003826"
     CDS             complement(101..796)
                     /locus_tag="OO448_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6338923.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="OO448_003827"
     CDS             complement(789..2216)
                     /locus_tag="OO448_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6338924.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="OO448_003828"
     CDS             complement(2227..2946)
                     /locus_tag="OO448_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6338925.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEALPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO448_003829"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO448_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCR6338926.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="OO448_003830"
     CDS             4554..5879
                     /locus_tag="OO448_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCR6338927.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="OO448_003831"
     CDS             5988..6224
                     /locus_tag="OO448_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6338928.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="OO448_003832"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="OO448_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCR6338929.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="OO448_003833"
     CDS             complement(6989..7858)
                     /locus_tag="OO448_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6338930.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1982 c         1733 g         2196 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa gcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DAJTVJ010000170         8093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000170 DAJTVJ010000000
VERSION     DAJTVJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.170"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..603
                     /locus_tag="OO448_003834"
                     /pseudo
     CDS             1..603
                     /locus_tag="OO448_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1217..1593)
                     /gene="rnpB"
                     /locus_tag="OO448_003835"
     ncRNA           complement(1217..1593)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO448_003835"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1626..2771)
                     /gene="garK"
                     /locus_tag="OO448_003836"
     CDS             complement(1626..2771)
                     /gene="garK"
                     /locus_tag="OO448_003836"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6338931.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2868..3758)
                     /gene="garR"
                     /locus_tag="OO448_003837"
     CDS             complement(2868..3758)
                     /gene="garR"
                     /locus_tag="OO448_003837"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6338932.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3788..4558)
                     /gene="garL"
                     /locus_tag="OO448_003838"
     CDS             complement(3788..4558)
                     /gene="garL"
                     /locus_tag="OO448_003838"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6338933.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4574..5908)
                     /gene="garP"
                     /locus_tag="OO448_003839"
     CDS             complement(4574..5908)
                     /gene="garP"
                     /locus_tag="OO448_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6338934.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6283..7854
                     /gene="garD"
                     /locus_tag="OO448_003840"
     CDS             6283..7854
                     /gene="garD"
                     /locus_tag="OO448_003840"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6338935.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            8003..>8093
                     /gene="prlF"
                     /locus_tag="OO448_003841"
     CDS             8003..>8093
                     /gene="prlF"
                     /locus_tag="OO448_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCR6338936.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVR"
BASE COUNT         2114 a         2126 c         1908 g         1945 t
ORIGIN      
        1 ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta ctaaaaataa
       61 cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag tttgtcgaaa
      121 gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg agatttgcaa
      181 ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa ttaacaaaag
      241 aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa ttaccagaga
      301 tgaaagaatt ctcatcagaa aaaaatatat gattacatcg atataatcat tgaagattat
      361 gaaaatatca aagaaaatgc ttatctggtc gatccaaaaa ttaattacac tatagattta
      421 aacatagaag actctagctc agaggaattc ctttttgata atagcctcga gaaagacgaa
      481 aattcttcgg acaattgctt tgaggtcgaa aaatataacg catatgaagc atataactca
      541 gagaatctat attttaccag agaggaatat acgtatgatt acgacctttt aaatgcaata
      601 tagacataat aacttcttac aaaatctata attaataccg tagatattca ttcatcttac
      661 cctgtgcacc aaaagcaaaa actcactgac gcgggtttga aactgatggc aaccgcaaaa
      721 atgcctgatg cgctacgctt atcaggccta cgccatctct acaatatatt gaatttgcgt
      781 gcttttgtag gcaggataag gcgttcacgc agcatccggc atgaataaag cgcactcaac
      841 aatctgaaac ccgccggaac ggtttattac gtacatcagg tgaaactgac cgataagccg
      901 ctttcttttg ggtatagtgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa
      961 aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag ccgctttctt
     1021 ttgggtatag cgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
     1081 cgaagcggtt ttttacgtaa accaggtgaa actgaccgat aagccgcttt cttttgggta
     1141 tagcgtcgtg gacagtcatt catctttccg ctcctccaaa agcaaaaacc cgccgaagcg
     1201 ggtttttacg taaatcaggt gaaactgacc gataagccgg gttctgtcgt ggacagtcat
     1261 tcatctaggc cagcaatcgc tcactggctc aagcagccta cccgggttca gtacgggccg
     1321 taccttatga acccctattt ggccttgctc cgggtggagt ttaccgtgcc acggactgtt
     1381 accagccgcg cggtgcgctc ttaccgcacc ctttcaccct tacctgatcc cgcttgcgcg
     1441 ggccatcggc ggtttgctct ctgttgcact ggtcgtgggt ttccccccca ggcgttacct
     1501 ggcaccctgc cctatggagc ccggactttc ctcccctccg cccgtctccc ccgaagagga
     1561 cgacgacgaa gcggcgactg tctggtcagc ttcggcgcgc agtatagagg gtttgcgcgc
     1621 ccttgtcacc ccgcgttgcg cattccaatc gccagtgtcg cggcgatatt acgtgaagca
     1681 cggcagatat tgtcataagc cccgcggaat gcttcgtcca acgtacctat gctggtcaat
     1741 acgctgaaga ccgcatcgat gccatgctga tgtacaacgc caacatcatc ggtcaggcta
     1801 cccgcaatgc caatcaccgg tttatggtac ttcttcgcca cgtttgcaac gccaatcggt
     1861 accttgccgt gaatactctg gctgtcaata cgcccttcac cggtgatcac cagcgtacaa
     1921 tcgtgaatat gttcttccag attcagcgcc gtagtgacga tttcaatacc acttttcagt
     1981 tccgcaccaa gaaacgccat cagcgccgcg cccataccgc ccgcagctcc tgcgccgggg
     2041 acatctttca catcaacatg cagcgctttt ttaatgacat cggcatagtg agagaggtta
     2101 ttgtccagct caacaatcat cgcttcactg gctccctttt gtgggccaaa gatgcgcgat
     2161 gcgccgttat cgcccaccag cggattggtg acatcacaag cgacgcgaat gacgcaatct
     2221 tttaagcgcg gatcgaggcc ggaaatatca atatcattca gagtattaag actaccgccg
     2281 ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg cccccagcgc ctgtaccatg
     2341 cctgcgccgc catcatttgt agcgctgccg ccaatgccga taataatgtt tgttgcactg
     2401 ctctccagcg cctgcaggat taactcgcct gtgccgcgtg aagtggttac gagtggatcg
     2461 cgtttttccg caggtaccag ctccagccca ctggccgccg ccatttcaat aaacgcggtt
     2521 ttgccatcgc cggagatccc ccaactggca ttcactttct cgcccagcgg ccctgtaacc
     2581 caggcgtgac gttcagcccc ctgggtggct gcaatcatcg cttccaccgt tccttcacca
     2641 ccgtcggcaa ccggaacaga aacatactgt gcatcaggaa aaatttcccg aaatcctttt
     2701 tctatcgcct gcgcaacctc gctggcagat aaactttctt tataagagtc tggggcgatt
     2761 acgattttca tacctatgcc tgttaccaca tgacgccgga gggcgtttct cttattcggc
     2821 ctagattcca ggcccggatt gcaatacacc atccgggcac gacgtcatta acgagtaact
     2881 tcgactttcg ccagtttttc gtagtagcac gccagggcgc tatgatccgc cgttcctaaa
     2941 ccatctgcac gcagtgcctg catcatctcc ataaccgcag ctgtgagcag cagttgtgcg
     3001 ccgacgccgt gagaagtatc cagcgcattc gccagatcct taatatgcag atcaatgcgg
     3061 aagcccggct tgaagttgcg gtccatcacc atcggcgctt tggcatccag cacggtactg
     3121 cccgccagcc caccgcgaat tgcctgataa accaggtccg ggttaacgcc cgctttggtt
     3181 gccagcgtta acgcttctga catcgcagca atattcagcg ccacaatgac ctgatttgcc
     3241 agtttggtga cgttacctgc accgatttcc ccggtatgca ccacggaacc cgccatcgct
     3301 ttcatcaaat catagtattt gtcgaaaata gccttgtcgc cgcccaccat cactgacagc
     3361 gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag catccagcat atcaatgcct
     3421 ttcgctttca gcgcttcgct gatttcacgg cttgccagcg gtgcgataga actcatatcg
     3481 atcaataccg tacctggctt cgcgccttca ataatgccat tctcacccag cgccacctct
     3541 ttcacatgag gggagtttgg cagcatggtt atgatgacgt cgcactgttc agcgatcgct
     3601 ttagccgtag acgctgtttc tgcacctgca gcaatcacgt cagcaatagc ttctgggtta
     3661 cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt ttttactcat tggtttaccc
     3721 ataatcccca ggccaataaa accaactttc atagtcatat caaccatctc tcttgttgcg
     3781 gtggtggtta ttttttaaag gtatcagcca gtttctgagt ggcagagcgg aagacgccaa
     3841 gatcgctgcc gacagccaca aacgtcgcgc cccattccag ataacgacgc gcatcggctt
     3901 cgaccggcgc gagaataccg ctgggtttgc cgtgcgcgct ggcacggtta aaaatatgct
     3961 gaattgcttt ttgtacatcc ggatgcgagg cattgccgag atggcccaat gccgcagcca
     4021 gatcgctggg gccgacgaag ataccgtcta caccttcggt agcggcaata gcatcgacgt
     4081 tatctacgcc ctgctgactt tctatctgga ccagaatagt aatgttcttg ttcgactgag
     4141 cgaaataatc cgccacggtg ccaaacatat tggtgcggtg agaaacggag acgccgcgaa
     4201 tgccttccgg cgggtaacga gttgatgcca ccgcctgctc tgcttcctct tttgtttcta
     4261 caaaaggaat caggaagtta tagaaaccga tatccagaag acgcttaata attaccggct
     4321 cgttggtcgg cactcgcact actggcgcgc tggcgctgcc tttcagggcc attaactgcg
     4381 gaataaacgt ggaaatatcg tttggcgcat gttcgccatc cagcaccagc cagtcaaacc
     4441 cagccaaacc aagaacttca gtgctaatcg ggttagagag tgctgaccag caaccaattt
     4501 gtacctgttt cgcagccagt gcggctttga atttattcgg gaaaacatcg ttattcatcg
     4561 cttatacctt tgcttatttc tgcaattcca tacgtttaat gtcgccaact acgaagaggt
     4621 agcagaccat cgccatcagc gctgaacatc ccacgaaaac cagtgctgca ttgaaggagt
     4681 gcagttcact taccaggtag ccaatcacca gtggagtgac aatggaggca acattgccaa
     4741 agacgttaaa gacgccgcca cagaggccaa caatctcttt cggcgcggtg tcagaaatca
     4801 ccggccagcc cagagcacca aatcctttgc caaaaaaagc cagcgccatc agcatgacca
     4861 ccagcgtggt gttgttggtg tagttacata agatgatggt ggaaaccagc aacattccca
     4921 gcacaatcgg tagcttacgt gccagggtca gggataaacc gcgtttgatc agataatccg
     4981 agaagacacc tcccagcacg ccgcccgcaa aaccacacag tgccggaatc gaggcgacca
     5041 gacccacttt cagaatcgac atgccttttt cctgcaccag ataaatcggg aaccaggtga
     5101 ggaagaacca ggtgatggtg ttgataaaat attgtccgaa aaatacgccc aacatcatgc
     5161 ggttagagag caattgcttg atgtaatgca gtttgggacc gcttgctgcc gcgctgcccg
     5221 gctttttgtg gtccatatcg accaccgcgc cattttcaga gataaacttc agctcttccg
     5281 cagacatacg tgggtgatct gtcgggttat gaatcaactt gatccacagc gccgtcagca
     5341 caaaaccaat cccccccata acggtgaaga cgtgctccca gccccaagcg aaagtcagcc
     5401 agccaagcag cggcgaaaag agcgccagcg agaaatattg cgccgagtta aagatggcgg
     5461 aggcagtacc acgttctttc gtcgggaacc aggcggcgac aatacgggcg ttcgccggga
     5521 atgatggcgc ttccgagaac cccagcataa agcgcataaa gaacatggag atccctgccc
     5581 aggccagcgg gaacatatca acaaagcctt gcaggaaggt gaacagcgac cagaaaaaga
     5641 ggctgtaggt gtaaactttt ttcgagccaa acttatcaag caaccagccg ccggggattt
     5701 gcatcagcaa gtaggcccag ccaaaagcag agaagatgta acccatcgaa accgcactta
     5761 actgcaactc ttttgccact tcggtaccag caatagacag cgttgcacga tcggcgtagt
     5821 taacggcggt aacaataaaa ataatcagta atattaaata gctggtatgc acgcctttct
     5881 tttttacgtc aacggtgtcc agaatcattt tatttacctc gggtacttat gctgattttt
     5941 attattatgg ggaaggtgtt atttatgagt tttatttatg ccgttacgac aatgaactcg
     6001 ggaattagta taagcagcgg gagaataata atcattgtgc aaatgctaat ttaatcagta
     6061 ctatttaaat attattttga gcatatgcac ataaggttgc gcgctaaagc acagatttgc
     6121 gctttacctt accgggcggc actgcaatcc ctgaaatgat tgacattgat cacatttctg
     6181 cgtttaaact cctgacattc ttatttcacc caatgaagtc atttattttt aaatgagacc
     6241 agatcctcat tttaataacc cctggctgga gaatattgca caatggccaa catcgaaatc
     6301 agacaagaaa cgccaactgc gttttatata aaagttcacg acacagataa tgtggcaatt
     6361 attgttaatg ataatggcct gaaagcagga acgcgttttc cggatgggct ggaattaatt
     6421 gaacatactc cccaggggca taaagtcgca ttgctggaca ttccagctaa tggtgaaatt
     6481 attcgttatg gcgaagtgat tggttacgcc gtgcgcgcaa tcccacgcgg aagctggatc
     6541 gacgaatcaa tggttgtact accggaagcg ccgccgttac acacgctgcc actggcaacc
     6601 aaagtcccgg cccccttacc gccgctggaa ggatacacct ttgagggcta tcgcaatgcc
     6661 gatggcagcg tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca ctgtgtggca
     6721 ggcgtggtgg actatgtagt aaaaatcatt gaacgcgatc tgctaccgaa atacccgaac
     6781 gtcgatggcg tggtggggct gaatcatttg tacggctgtg gcgtggcgat taacgcaccg
     6841 gcggcagttg tgcctattcg taccatccac aatatttcgc tgaaccctaa ctttggcggc
     6901 gaagtaatgg tgattggtct gggttgtgaa aagttgcagc ctgagcgcct gctggtcggc
     6961 acggatgatg tgcaagctat tccagtagaa agcgccagca ttgtcagttt gcaggatgaa
     7021 aagcatgtcg gctttcagtc catggtcgag gatattttgc aggtcgccga acgccatcta
     7081 caaaaactga atcaacggca gcgagaaacc tgcccggctt cagaactggt cgttggtgtg
     7141 cagtgcggtg gcagcgatgc gttttctggt gtaacggcaa acccggcggt tggctatgcg
     7201 tctgatctac tggtgcgctg cggcgcaacg gtgatgtttt cagaagtaac ggaagtgcgt
     7261 gacgcgatcc atctgctgac accacgcgca gtgaacgaag aggtcggcaa acggctgctg
     7321 gaggagatgg agtggtacga taactatctc aatatgggaa aaaccgaccg cagcgccaac
     7381 ccttcgccgg gcaacaagaa aggcggtctg gcaaatgtgg tggagaaggc actcggctcc
     7441 attgctaaat cgggtaaaag tgcaattgtt gaagtgctgt cgcccggtca acgcccgact
     7501 aaacgcggat taatttacgc cgcgacgcca gccagcgatt ttgtctgtgg cacgcaacag
     7561 gtggcttcgg gtatcaccgt acaagtgttt acgaccggcc gtggtacgcc gtacggcctg
     7621 atggcggtac ccgtaattaa aatggcaacc cgcaccgagc tggcgaaccg ctggtttgat
     7681 ttaatggata ttaacgcggg cactatcgct accggcgaag agagcattga agaggtgggc
     7741 tggaagttgt tccactttat tctcgacgtc gccagcggga agaagaaaac cttctcggat
     7801 caatgggggc tgcataacca gctggcggtg tttaacccgg caccggtgac ctgatttctt
     7861 ttggaacgtc cctcgcaaaa catggcctta gtgccatgtt tttattgttt aaagccccca
     7921 cgtccattaa taatgcattt gcattacctt agttcaagct tataatttga gcagaaaaca
     7981 ggctgtaaaa ggacaatgaa tcatgcccgc taatgctcgc tctcacgctg tactgaccac
     8041 tgaatcaaag gtcacgatac gcggacaaac aactatcccc gcgccagtgc gtg
//
LOCUS       DAJTVJ010000171         7945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000171 DAJTVJ010000000
VERSION     DAJTVJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.171"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            109..441
                     /locus_tag="OO448_003842"
     CDS             109..441
                     /locus_tag="OO448_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6338937.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            588..1949
                     /gene="yegQ"
                     /locus_tag="OO448_003843"
     CDS             588..1949
                     /gene="yegQ"
                     /locus_tag="OO448_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6338938.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2279..2596)
                     /locus_tag="OO448_003844"
     CDS             complement(2279..2596)
                     /locus_tag="OO448_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338939.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3002..3901
                     /gene="yegS"
                     /locus_tag="OO448_003845"
     CDS             3002..3901
                     /gene="yegS"
                     /locus_tag="OO448_003845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6338940.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3983..4756)
                     /locus_tag="OO448_003846"
     CDS             complement(3983..4756)
                     /locus_tag="OO448_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6338941.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4862..5902)
                     /gene="gatD"
                     /locus_tag="OO448_003847"
     CDS             complement(4862..5902)
                     /gene="gatD"
                     /locus_tag="OO448_003847"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6338942.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5950..7305)
                     /locus_tag="OO448_003848"
     CDS             complement(5950..7305)
                     /locus_tag="OO448_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6338943.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7309..7593)
                     /gene="gatB"
                     /locus_tag="OO448_003849"
     CDS             complement(7309..7593)
                     /gene="gatB"
                     /locus_tag="OO448_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6338944.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7624..>7945)
                     /gene="gatA"
                     /locus_tag="OO448_003850"
     CDS             complement(7624..>7945)
                     /gene="gatA"
                     /locus_tag="OO448_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6338945.1"
                     /translation="AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQAD
                     DDNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVL
                     DSDE"
BASE COUNT         2148 a         2037 c         1783 g         1977 t
ORIGIN      
        1 acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat cggtaaacag
       61 cacctttaga tgctgttttc gatacgcaat ttcaatcaag gagtcattat ggctggttgg
      121 tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc gggcaatggt
      181 gagactatcc tcaccagcga gctgtataca tcaaaagcct ctgcggaaaa gggcatcgcg
      241 tcggtgcgta gcaacagccc gcaagaagaa cgctatgaga aaaaaacggc aagtaacggc
      301 aaattctatt tcaatctgaa agccgccaat catcaaatta tcggctccag ccagatgtac
      361 gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg cacaagccag
      421 acggtgaaag acaatacgta attacgatgc cgggcgtggg gtgtcggcgt ccggcgcttt
      481 accagatccc cccacagcgc tacaatgccc gcccttaaag tgggggcact cccctaaccg
      541 cttcatcagg tgaagcggag ctgacctgtc atcagaacga gagaattatg tttaaaccgg
      601 aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct tatggcgcag
      661 atgctgttta tgcgggccag ccgcgttact ccctgcgtgt gcgcaacaac gaattcaacc
      721 acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag ttttatgtcg
      781 tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac ctgaaaccgg
      841 tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt atgctggtgc
      901 gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc gtaaactggg
      961 cgacggtgaa attctggcag caaatgggcc tgacccgcgt gatcctctct cgcgagctgt
     1021 cgctggaaga gattgaagag atccgcaatc aggtgccgga tatggagatt gagatcttcg
     1081 ttcacggtgc gctgtgcatg gcctactccg gtcgctgcct gctctctggc tatatcaaca
     1141 agcgcgatcc gaaccagggt acctgcacca acgcctgccg ctgggagtac aacgtccagg
     1201 aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt ccggtgcaaa
     1261 atgttgagcc gacgctaggt atcggcgcgc caaccgacaa agtgtttatg atcgaagaag
     1321 cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact tacatcatga
     1381 actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg ggcgtgcatt
     1441 cgctgaaaat cgaaggccgt actaaatctt tctactattg cgcacgcacc gcacaggttt
     1501 atcgtaaagc tatcgatgat gccgctgcgg gcaaaccgtt cgataccagc ctgctggaaa
     1561 ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt catactcacg
     1621 acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag tttgttggtg
     1681 agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat aaattctccg
     1741 ttggcgacag ccttgagctg atgacgccgc aaggcaacat taactttacc cttgagcaca
     1801 tggaaaacgc caaaggtgaa gcgatgccgg tcgcaccagg cgatggttat actgtgtggc
     1861 tcccggtccc gcaggatctt gagctaaatt acgcgctgct gatgcgtaat ttctccgggg
     1921 aaaccacgcg taacccacac ggtaagtgat taatttcgat tatttttccc ggatggaaaa
     1981 ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt ttcgctgaaa
     2041 aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg tgtaatctcc
     2101 cttacacggg cttatttttt atgcataagc cctatccctg gtcaccgtct tccattgacc
     2161 acatcgatag aatctccctt catagcacga tgcctttcac gtaacggcat cgtgctcgca
     2221 caggttccgg ctaagcacaa ccagaacgcg catgtttgat gcttaccaaa aaatattctc
     2281 actctccaca tttgaatgtc agacgagcga cgccatgtaa tcctgcacct tctgtcttca
     2341 ggtcaactat ctgcattttt ttgccctgag taacacagaa atgggctgca tcatttttta
     2401 ctatattttc tgcaccagat attcttcccc tagctaaaga agcttcggct tcggtgtagt
     2461 attggttatc gagtttacgc tgaatattac ttttatatgc aagaccaaat ttaccgatac
     2521 ttgtttcatc attatgcaca gcacaaccag atataagaaa aatactaatt aatgatatag
     2581 cagctatctt tttcatctca ccttccccca ttaaatacca acgacactct ctagtgttta
     2641 aatataataa tggcatgatt attataattg aataggatta taataaatgt tctgtacaac
     2701 atttcctaca taagtaggaa ttacggacat tgaggccctt cagggtaact ccatgggggc
     2761 tttaatatat tatattgaag atgccactgt ttagttgaat attaggtata tgctcttttt
     2821 tgaaatttat cggtggcagc cgttagtatt cgctgtcccc attgcaagct cctggtggta
     2881 accactgaat cctccatact tgaactgact ttttatcctc cgactttcat cctgttctga
     2941 ctccaccttt tgttttctgc tctacactat ctacagacca atcataaagg cacatacgat
     3001 catggcagaa tttcccgcca gcttactgat tcttaatggc aaaagtactg acaatctacc
     3061 cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg acgatccatg tgcgggtcac
     3121 ctgggagaaa ggcgatgccg cacgatatgt agaggaggcc cggaagttgg gcgtcgcaac
     3181 ggtgattgcc ggtggtggcg atggcaccat taatgaagtt tctacggcgt tgattcagtg
     3241 tgagggggat ggcatacccg cgctgggaat tttgccatta ggaaccgcca atgattttgc
     3301 caccagtgta gggattcctg aggcactgga taaggcgctg aaactggcaa ttgccggtaa
     3361 cgccattgcg atagatatgg cgcaggtcaa caaacaaacc tgttttatta atatggcgac
     3421 aggcggattt gggacgcgta ttaccacaga aacgccggaa aaattaaaag ccgcgctggg
     3481 tggcgtctct tacatcattc atggcttaat gcgcatggat actctgcaac cggaccgttg
     3541 tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc ctggtcattg gtattggtaa
     3601 cgggcgtcag gccggtggcg gtcagcaatt gtgtccgaac gcgttaatta acgatggctt
     3661 gctgcaactg cgcattttta ccggcgatga aattcttccg gctctcgtat caaccttaaa
     3721 atctgacgaa gataacccga atattatcga aggcgcttcg tcgtggtttg atatacaagc
     3781 cccacacgaa atcactttta atcttgatgg cgaaccgttg agcggacaaa actttcatat
     3841 tgaaatactt ccggcggcgt tgcgttgtcg attaccacca gattgtctat tattgcgtta
     3901 atcaaactat tctctcttga accagggaga atccgaaatg ccggatgcgg tgtaaatacc
     3961 ttatccggca tacagaacaa tactacaccg taattaattt tacccgtgtc ttctgcaatg
     4021 ccagtttatc cacctctgat acagcatcat cggtaataat aatgtcaatt ttttcgattg
     4081 gcagtacctg attaaaaccg cgacggttaa atttcgatga atcgagaacg gcaatcactt
     4141 tattagcggc ggtgaccatc gcaccgctaa tcgaataacc ttcattaaag gtcgtaatac
     4201 cattaaccgc atcaatacca tcagcaccga caaacattaa atcggcatta atatcctgca
     4261 atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt atggcggacc gtgccaccac
     4321 agacgaccag agtaatgtct ttattttcgg aaagggcaaa cgctgccggg agactgttgg
     4381 taatcaccgt gatattttta gcggtcatta atccttcggc gataagcatt gtcgtacttc
     4441 cgctgtcgag aataacggtc atcccttcat ggatcatttt gactgccgcc tgcgctattc
     4501 tgttttttgg cgcgctggcg agctgaaatc gctctttaaa cccgacttcc tgggtctcgg
     4561 tttcactatt accggacatt attttcgccg caccgccatg aaagcgcgta accacgcctt
     4621 tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca aatactcccg
     4681 ccagatcctg aacaagcacg gttccctgtt cattcactaa ttggatgatc ttattccttc
     4741 gctcgaatga attcatcgtc atttacccat attcaattgt ggctagtgta aacgaagggg
     4801 gaccattggt gaataatctg ttttgcacaa taccgggcca gcatcacgct ggcccgcggt
     4861 ttcatggaat gagcaacact ttgcccggca tggcattacg agcgatgtca cgcaccgcct
     4921 gggtgaagct ttcaaagctt ccacggtgag cgattaatgg ctccaggctt aacttacgtt
     4981 ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg ccaagggctg gaatagttca
     5041 tccaactgcc gataaccgtc agctctttac gcaatatttt gccaaacgtt gccgatgtta
     5101 aatgcagatc ctgatgcaac gtgcccacca gcgccagttg ggcatgagga ccggcaatct
     5161 ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc gaggataagc tgattaaagc
     5221 gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt
     5281 gcatcgcacc gaaagatttt gccaatgcca gtttttctga gctaatgtcg atcgccgtca
     5341 cactctttgc tcccagcgcg acagcgcact gaatagccag caggccaatg gttccggcac
     5401 caataataat aacgttttta ttctcacaac cttgcgctaa atgaaaagca tgcaggccaa
     5461 cggtaatcgg ctcaataaaa gccccatcct caataggcat atccgtgggt agagcaaaga
     5521 cattttttcg cttaacgaca atatattcag caaatccacc atcacgccgc gagccaataa
     5581 aatcatattt tgcgcactgg gaataaaacc ctttcaaaca ctctggataa gtaaaacagg
     5641 gtaataacgg cacacaggca accgcatcgc cagggtgtaa atcatcaaca ccggatccca
     5701 ccgcatcaat atagccgcta aattcatggc ctaacgttat tggataataa tgtgcaccat
     5761 ttttaaatat cctgggtaaa tcggaaccac ataagcccga gctggcaatt tttacccgca
     5821 cctcatcctg atgtttaatt tcaggaatga cgctttctgc aacgcgcacg ataccatcag
     5881 tatcattcac cattgatttc ataaaaactc ctgattgtta agggggataa cctccccctc
     5941 aggtaaaaat tattctgcga gaacggtttt ctcttgttta ataaaaccac gcgctctacg
     6001 ccaggtcatg aaaataccgg taagataaat tgcgccgata ataatgaaac cgggaatatt
     6061 ttgcgtggag aaaacctgaa tcagtaacca ggtaatggga gaaccgccct gatccattga
     6121 agccaccata cccccggctt ttaacgcccc agcattagcc gccagttggg tgtgcaaacc
     6181 aatagtttgc gtcgcgatcc acagggtgat gctcataatg atgacacccg agattaaggt
     6241 gcggaacaga tttccacgat gcaccgcgac cgccatcgcc acgaagaagc cgatggtggc
     6301 aagatcgcca aacggcagta cctgattacc cggaacacaa acagcaatta aaatggtgag
     6361 cgggataaaa atcaggcttg ccgataccac cgccgtatgc cccagcagta atgcgggatc
     6421 caggccaatc aggaactcct gaccgccgaa cttcgcctgt aaacgactac gggcctgctt
     6481 agcgatgggc gttaaaccat ccatgatggg tttaatcacc cgtggcatca gcagcataac
     6541 tgccgccgtt tttaccgcca gctgcaatac acctttgaca tcgtaaccgg cgaggatgcc
     6601 gataatcagc cccatcacaa aacccacggt gacaggctcg ccaaatggac caaatttgcg
     6661 ctgaatatcg tcggcgctaa atttaattcg gttaacgcct gggattttct cgatgatagc
     6721 atcgaccagc accgcaatcg gccccatata cgccgacgta ccgtgcggaa tagcaatacc
     6781 ttccagctca aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa
     6841 cgccgcgtga attaccacac ctgccatccc tatcatccat gaaccggttg ccagatgcag
     6901 caacgcgccg gtgaaggtca tatgccagat attccagata tcaacattta ccacccgcgt
     6961 catacgggtc agtaacatcg ccacgttaac cagaatcgca atcggaatcg ccaccagcgc
     7021 aatttgcgac gcccaggtca ttggtgaaga gcccggccag ccgacatcga ccacatgcag
     7081 attcaggtcg aaattttccg ccatcgcttt cgccgccgga ccaatggaat ccagcattaa
     7141 gccaatcaca aggccaatgc caacaaagcc aatcccgata tgcagacccg ctttaaagca
     7201 atcgcctgcc ttcatgccta atattttaga aaaaataatg atgacaatcg gaagcatcac
     7261 cgtagggccg aggtcgagaa tataacgcat gacttctgaa aacataggtc acccctgtaa
     7321 gatagtcaga attttatttt gtaatgcttc gataccgaca ccagaaataa aaggcatgcc
     7381 gtgaactaac ggaatatcgc caaaactacg atccactttg gcagtggtgc atatcaaatg
     7441 cacaccatcc atataggttt ctatttcatt aacccgacac tggattaatt caacaggaat
     7501 actatgattc tgacacaact ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc
     7561 gcctccgcaa gcgacaataa tcttgcgttt cataaatagt cctttttatt gttacagagg
     7621 gatttattcg tctgaatcta aaacatactt tgtgaagtat tcctttaact gggtttcagg
     7681 aagagtgatt agtgtctcga cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa
     7741 aagtttcaat tgctgctgcg gattctccac aatcaacgca ataaccaacg atacctccac
     7801 gtcgttatca tcatccgctt gctgaaaatg aactttattt gttggcctta acagataaat
     7861 ggctgacgac ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt gctgctcaag
     7921 cattatcccg gtagggaatt ctgct
//
LOCUS       DAJTVJ010000172         7789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000172 DAJTVJ010000000
VERSION     DAJTVJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.172"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(326..1162)
                     /locus_tag="OO448_003851"
     CDS             complement(326..1162)
                     /locus_tag="OO448_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6338946.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1162..1965)
                     /locus_tag="OO448_003852"
                     /note="aph(3'')-Ib"
     CDS             complement(1162..1965)
                     /locus_tag="OO448_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6338947.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2026..2841)
                     /gene="sul2"
                     /locus_tag="OO448_003853"
     CDS             complement(2026..2841)
                     /gene="sul2"
                     /locus_tag="OO448_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6338948.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4030..4179
                     /locus_tag="OO448_003854"
     CDS             4030..4179
                     /locus_tag="OO448_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338949.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(4935..5375)
                     /locus_tag="OO448_003855"
     CDS             complement(4935..5375)
                     /locus_tag="OO448_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6338950.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            5593..5982
                     /locus_tag="OO448_003856"
     CDS             5593..5982
                     /locus_tag="OO448_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338951.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6026..6214
                     /locus_tag="OO448_003857"
                     /pseudo
     CDS             6026..6214
                     /locus_tag="OO448_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6181..7380)
                     /gene="tet(A)"
                     /locus_tag="OO448_003858"
     CDS             complement(6181..7380)
                     /gene="tet(A)"
                     /locus_tag="OO448_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6338952.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            7486..>7789
                     /gene="tetR(A)"
                     /locus_tag="OO448_003859"
     CDS             7486..>7789
                     /gene="tetR(A)"
                     /locus_tag="OO448_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6338953.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHA"
BASE COUNT         1793 a         2117 c         2128 g         1751 t
ORIGIN      
        1 tccagggcgg ccgtcttcac gttctgcctt gcgcagatga gatagatcta gcgtggactc
       61 aaggctctcg cgaatggctc gcgttggaaa ctttcattga cacttgaggg gcaccgcagg
      121 gaaattctcg tccttgcgag aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg
      181 gcttgtctcg cccctctccg cgtcgctacg gggcttccag gggtcgtttg cgggaagggg
      241 cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga tgtgttccca
      301 ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg
      361 ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct gcggaaaggc
      421 acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc gcacgagaga
      481 atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg
      541 gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg acgggatcta
      601 tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg ccatgtagcc
      661 cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg tggacgtagt
      721 cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat
      781 cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac agcttcgcca
      841 ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga
      901 gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga
      961 ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca tcagcaatgt
     1021 cttctatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat gaaactttcc
     1081 aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc caatgagcag
     1141 gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca gatagaaggc
     1201 aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag cgaaggcgcg
     1261 ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga gtgccaaatc
     1321 ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc attgaagagt
     1381 tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa ccatatcggt
     1441 gcgctcttgg tcgagccgca ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg
     1501 cggcgtactc ttgtcctcgt ccggtaagaa gtcgggattg acggcattgc gggacaccac
     1561 atcaacggcg cgtccgaaca ttcgcgacag cctgcgctca aacggacatt gatcaaccga
     1621 taggctgtga acagcgccaa gttgctgccc cattgacggc cacgctttga gcaaatccgc
     1681 tccagacaga tcagccgccg gtactcccgg aattgccgtt atcaccaagc atgcaccctc
     1741 ctgttcctcc tgccagttga tgacctcggg gcaagccaca cctcgacctt tgagccaaat
     1801 gaggcggtca cgctctccag cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa
     1861 ggcatgcccg tcaccacgtc gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa
     1921 ccagtcagaa tgcgattcac caaaaaaaat attagttcga ttcaatggag gttccttcag
     1981 ttttctgatg aagcgcgaat atagagaaat atcccgaatg tgcagttaac gaattcttgc
     2041 ggtttctttc agcgccgcca ataccgccag cccgtcgcgc aaggggcgcg gctcgtgtgt
     2101 gcggatgaag tcagctccac ctgcggcggc ggcaagctct gcagcgagtg tcgcggcccc
     2161 gacatccccc ggaccacggc ctgtgagcgc gcgcagaaag gatttgcgcg aaacagacag
     2221 aagcaccggc aaatcgaagc gcagccgcaa ttcatcgaac cgcgccagca ccgagagcga
     2281 ggtttcggga gcagccccca gaaaaaaccc catgccggga tcaaggacaa ggcggttgcg
     2341 tttgataccg gcacccgtca gcgccgcgat gcgcgcgtca aagaacgccg caatgtgatc
     2401 catgatgtcg ccagcgggtg cctcgcgccg atctgcctgc ccgtcttgca ccgaatgcat
     2461 aacgacgagt ttggcagatg atttcgccaa ttgcggatag aacgcagcgt ctggaaaacc
     2521 gcgaatatca ttgagatagg ccacaccacg cgacaaggca taggcttgcg tcgcgggttg
     2581 ataactgtcg agcgagacgg gaatgccatc tgccttgagc gcgtccagca ccggcgcgat
     2641 acgcgcgatt tctgtgtcgg acgaaacagg cgcggcgtcg ggattgctgg atgccggacc
     2701 gaggtcgatc acatctgccc cctcggccat cagcttacgc gcctgcgcaa tggctgcgtc
     2761 tggcgccaga taccggcctc catcggagaa actgtccgag gttatgttga cgatgccgaa
     2821 aatgatgagc gatttattca tgggggcttc tataataata ataatcgagc atgagtctca
     2881 tacggatgct cgggtcgaaa gggaatcccc aggcgagtaa cctgtttgcg gtgatccgtt
     2941 tcatgatgat gtatctgtag cattttggaa ataaatatat catctctcaa taagtagata
     3001 tatttatctt ttttgcttct ttggtgctgc aagtcaccaa tacgagggaa acctgttacg
     3061 gagcggttta accgtaatca acagtgactg gtgtcatgtt cggaagatta attgatagta
     3121 atctacaaag gatcttcttg agatcctttt caatgaatcg taatctcttg ctctgtaaac
     3181 gaaaaaaccg cctggggagg cggtttgttc gaaggttaag tcagttgggg aactgcttaa
     3241 cccggtaaca gggttcacga gaccgccatt accaaaactg ttctttcagt gtagccgcag
     3301 tcaggcctct acttcaagac ctcttaatct atcttctcgt gcactcctgt tactaatggc
     3361 tgctgccagt ggcgttttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     3421 caaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     3481 tgccttcacg gaactgagtg tcaggcgtgg aatgagaaac cgcggccata acagcggaat
     3541 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac cagggggaaa
     3601 cgcctgggat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttt
     3661 gcgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg ccttatctct
     3721 tctttgctaa gtgtcttcct ggtatcttcc aggaaatttc cgtccccgtc cgtaagccgg
     3781 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
     3841 tctcctgtat cgcatattct gctgacgcgc cggtgctgcc ttttttctcc tgccacatga
     3901 agcacttcac tgattttcgc aatcgtggca acatagtaag ccagtataca ctccgctagc
     3961 gctacatgac tggttcaggg ctgcgccccg aaacccgcta acaacctctc agctactgga
     4021 tatcacccca tgccatctga atatgatgaa caactggatt cgactgatac gcagcttccc
     4081 ggcgaatatg acctttttga tttaatggcg caggttactg aagagaacct gcatgcccct
     4141 gtggattttg ggtcgcctgt cggccgggaa gcgttatagc tttttcgctt gcccgcgtac
     4201 gtggtaatac tccggtatta ccttttattt ctttattttc atgtaattac agtggatttt
     4261 taatacccgg gtattactcg tgaaaacgaa atattccttt tttttctttt aattcatgca
     4321 gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat tttgaggttg aaaaataatc
     4381 aaccatggcg ggtgatttac gcaggttaag gggagatcgt gacaaaaaaa gcgttcctca
     4441 ctgtatcgtt catgcctgca tgaacgggtt attcttcctg actacagaat tacatcgctc
     4501 cttcagttca tacatcactt ttacatcact tatacatcac ttatacatca cttttacatc
     4561 attccttcag ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct
     4621 gaacgtgctg acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa
     4681 ttgcgtcccc gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc
     4741 tgaatggtgt gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac
     4801 agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc
     4861 gtcagaaacg ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag
     4921 agtcacctgc actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt
     4981 ttttcctgtg ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc
     5041 tcatttcgca ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca
     5101 gtctgctgca gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg
     5161 ctaacgacag atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc
     5221 tcgccatagg cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg
     5281 cgatagctga caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt
     5341 aatttcagtg tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt
     5401 accgtattgt aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt
     5461 gaggcgtgaa aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt
     5521 cgaatactgt atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa
     5581 gaggataatt ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg
     5641 ggaccaaatg acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt
     5701 aacggtggtg gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt
     5761 ggtctcatca ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc
     5821 gatctttctg ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac
     5881 ctagatactg cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca
     5941 acggcaggca tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa
     6001 acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga
     6061 tcgggatact ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt
     6121 tgacgagtat tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg
     6181 ctatgtgcaa cgggaatttg aagaatttct ccaaagcccg cgacgcagcg ccggcaggca
     6241 gagcaagtag agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc
     6301 cgcatagatc gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc
     6361 cagtgagcct tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg
     6421 cagcgccggc attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca
     6481 tccccgtgtc gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca ttccgagcat
     6541 gagtgcccgc ctttcgccga gccgggcggc tacagggccg gtgatcattg cctgggcgag
     6601 tgaatgcaga atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa
     6661 gcgatcctcg ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat
     6721 gaagaagacc gccatcaggg cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc
     6781 gagcgggttg agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa
     6841 aaggaaacag cccgtcagga aattgaggcc gttcaaggct gccgcggcga agaacggagc
     6901 gtggggggag aaaccgccca tcagcccacc gagcacaggt cccgcgacca tcccgaaccc
     6961 gaaacaggcg ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc
     7021 aatataagcg ccggctaccg ccccagtcgc cccggtgatg ccggccacga tccgcccgat
     7081 atagagaacc caaaggaaag gcgccgtcgc catgatggcg tagtcgacag cagcgccggc
     7141 cagcgagacg agcaagaccg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg
     7201 tgcgcaggca aattgcatca acgcatacag cgccagcaga atgccatagt gggcggtgac
     7261 gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat
     7321 gccgacagcg tcgagcgcga cagtgctcag aattacgatc aggggtctgt tgggtttcac
     7381 gtctggcctc cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga
     7441 taagttggtg gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag
     7501 ccgaatacag tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg
     7561 acgacacgca aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc
     7621 aggaacaagc gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg
     7681 cattcggtgc cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc
     7741 ttcaggcagg cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccg
//
LOCUS       DAJTVJ010000173         7780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000173 DAJTVJ010000000
VERSION     DAJTVJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.173"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1783)
                     /locus_tag="OO448_003860"
     CDS             complement(<1..1783)
                     /locus_tag="OO448_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6338954.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQNR"
     gene            complement(1924..3264)
                     /gene="gntU"
                     /locus_tag="OO448_003861"
     CDS             complement(1924..3264)
                     /gene="gntU"
                     /locus_tag="OO448_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6338955.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3268..3795)
                     /gene="gntK"
                     /locus_tag="OO448_003862"
     CDS             complement(3268..3795)
                     /gene="gntK"
                     /locus_tag="OO448_003862"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6338956.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3934..4929)
                     /gene="gntR"
                     /locus_tag="OO448_003863"
     CDS             complement(3934..4929)
                     /gene="gntR"
                     /locus_tag="OO448_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6338957.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5153..5848)
                     /gene="yhhW"
                     /locus_tag="OO448_003864"
     CDS             complement(5153..5848)
                     /gene="yhhW"
                     /locus_tag="OO448_003864"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6338958.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5971..7008)
                     /locus_tag="OO448_003865"
     CDS             complement(5971..7008)
                     /locus_tag="OO448_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6338959.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7342..>7780
                     /gene="yhhY"
                     /locus_tag="OO448_003866"
     CDS             7342..>7780
                     /gene="yhhY"
                     /locus_tag="OO448_003866"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6338960.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1979 a         2102 c         1872 g         1827 t
ORIGIN      
        1 aacggttttg ctgcattttc tgcggtggat tgtccacgac tttttcaatg gcatcaataa
       61 aaggtaagcc aagtttcgcc gccagtcgcc gggcaaaatc aggaaccagg tcgaggtgct
      121 ttaatgaagg aacgcagcat acccaggttg gttcaggatg cggattccag cgttggcgaa
      181 ccatttccgc acatgcctcc accagctcat cactgaatcg ccccgttttt ttctcctgtg
      241 ctacctgctt tccccacccg gaatctcccc aacgagaaag gactcgcccc gtagatcctt
      301 gtaaacccgc aggcaagttc cctttaaatc cgtattgcgt aaaagctcca gatgctacct
      361 gtttattgag atttaatggc aggtcagcgt gtttaataaa taaatttgcc gcatggagta
      421 agccgctatc aatatcgggc gacaacaacg gatgctgaag acaactgcta catttaccac
      481 aacgttcggc acttggctca tcgagtgcgc ggcgcagaaa ttgcattttg cactccttgg
      541 tcgtgatata gctttcaaca tctgccagct cattttttct ctgattcatc aaatggttaa
      601 ttcgttcatg gtcaggagag aaactgacga tagttctgcg ccataatttc tcggtataca
      661 ccacaggcga tggattttca gctaccaata atttcagtgc tttttctatt tgcccataac
      721 ggagattcgt ccgttgttca atgcctcgta gggtaagacc gtcattctcg ctaagtacgt
      781 tgagtatttc atgaatttgc gcctccgcag gaaaggcgct ttcacggaag aatttatgaa
      841 tagcgcggtc ttcaccacca caaagcaata tgccaactgc ggaatctata gcacgcccgg
      901 cacgccctac ctgttggtag taaccgacaa tagaccccgg catttgataa tgaatgacga
      961 aacctaaatc aggtttatca aaccccatac ctaatgccgt cgtcgcgacc agcactttga
     1021 ttttatttgc cagcagcgcc tgctccagat attccctggc agtattagaa tcttccgcgc
     1081 cttcacaagt cacgccgctg tagtaagcaa atgcactgat accatttgtc cttaaccact
     1141 cggcgacaag ttcggcatcg cgagtagtca gggtataaac aattcccgat ttggaaaact
     1201 gagggataac cgttgctaac catgccagac gggatgactg ttcacctaag actaaggcat
     1261 caagcgccaa actttcgcga gccagcgttc cacgctgaat cacaatgtca cccaattgct
     1321 gacggatatc ctcaacgaca cgattattcg ctgtcgcggt tgtacccaga acaggggtat
     1381 tcgcagtttc aagaaaatcc tgattcgcca accgttcagg tgaaatcagc agacaatcta
     1441 caccaccccg cagcaactta tcgctaatgc gctgccattc ttccctgttt gtagaattca
     1501 acgtttcagc cgtaatacct aaccgttcag cggcggcaac ctggttacgc atcagggcaa
     1561 gcaaaggaga gatgataatt gtcgggccag cgccgcgatc gcggaagatt ttactggcga
     1621 tgaagtacac ggcacttttc ccccagccag tgcgttggac aaccagcagt tttcgccgct
     1681 gattaaccac cgcatctata gcttcccatt gcccttccct gaaagtagcg ctggtgttac
     1741 ttaacatccg ctgtaacagc aattcagctc cgtgtttctc catcatcttc tccctcgtcc
     1801 gttaacgcag tgattagaac ggataatgtt ccggtctttt tgcgagagag atcaaaaaga
     1861 tgaagacagg agataagaga aatgaattac tgcgagcaag ctcgcagtaa tttgtcgcag
     1921 taattaactc aacagctgga acgcaatcat cccaacaatg gcaccaacag tgccgaggat
     1981 ggtttccatc atggtccagg ttttcagcgt ttcggcttcg gtcgcgccgg taaatttacc
     2041 gaacaaccag aaaccagcgt cgttaacgtg gctgacaaca atcgaaccac cggcgataca
     2101 aatcgacagc gccgccattt gcgcaccgga attgttcagt tgttcaatga ccggcatcac
     2161 cagccccacc gccgttaaac aggcaacggt ggcagacccc tgaatgatgc gcactgcagc
     2221 tgccagcacg aagcaggtga tagcaatcgg caggcccatg ccggttaacg cttcgcccag
     2281 tgccggacct acgccagagt caaccagcac ctgtttgaac acgccgcctg caccaatcac
     2341 cagcagaatg atccccgccg gttgcagcgc gtgaccgcaa atctccatca ctttgtcttt
     2401 tggcatgccc tgacgcattg ccaggccata aatcgctacc agacaagcaa ccagaatcgc
     2461 ggtaaacgga tgaccaataa actcgaacca ttcgtaagcg gtagagcctt ctggcacaaa
     2521 acgcgcggca atggttttca gccccaccag caccagcggc aacaggatca gcgacaggct
     2581 gaatccgaaa gatggcattt taccttcgcc gagatgcggc tcgttgatgt cgtcaggaat
     2641 atgcagctca acgtaacggc tgatgaagtt accccacagc ggcccggcaa taatcattcc
     2701 cggaattgcc gcacacaggc caatcaggat catccagcca aaatcggcgt tcatctgcga
     2761 tgccagcagc attggcgctg gccctggcac caggaacgcc gccgctgccg ccacgcctgc
     2821 gaataatggg attatcagct tcaccaggtt cgtatcggtg tggcgcgcca ttgagaaagc
     2881 aacgctaatc agcagaacaa tcgccacttc aaagaacagc ggtagcgcac agaccagccc
     2941 cgcaaggccg atggcataat gcgcgcggct gtgaccgaag gatttgagca ttttgacggc
     3001 aatctgatcg actgcgccgg tttcatgtaa gatcttgcca aacatagctc ccagggcgac
     3061 aaccaccgcc aggaagccga gggtgcctcc catccctttt tccatcgtcg ctgcgatttt
     3121 atcgagcggc ataccggaaa aaaggccagc ccccatggac accaccatca aagccaggaa
     3181 agcgtgcata cgcgccttca tgactaaaaa cagcagcagt aaaacagacc ctactgctgt
     3241 taaaacaagc gttaatgtag tcactactta tttgcctttt ttaataacct caatggtgct
     3301 tgccacaaca ccttccagcg gttgatcgat atccaccacc agtacatcgg tttcgtccgc
     3361 acccggctcc tgcagcgttt caaactgcgt caccaacatt tgggttttaa agaaatggcc
     3421 tttgcgcgct ttcaggcggc tttcaatcac gtcaaaatcg cctttcaggt agatgaaaga
     3481 gagattcgga ttaccttcac gcagcaagtc gcgatagtgt tttttcaatg cagaacagac
     3541 gatcagtgac actttattag tgcgctgcat agcaaacgcg gcgtcgttca gcgcctgcaa
     3601 ccagggtttg cgatcgtcgt cattcagcgg ttcgccagac gccatttttt cgatattgcg
     3661 ccgtggatgg aggaaatcgc catcaagaaa cgcggcatga agttgatgcg ccacttcact
     3721 ggcgaccgca gatttgccgc tgcccgatac gcccatcaag acgtaaatgt ggtgatcatg
     3781 gttagtcgtg ctcaaagtgg tgcccccaca atacaagaat taaaaatgtt acgggtaact
     3841 gttatcggta acattgtcca gccggacaat atcagaagca atatccatcc gtgccttaag
     3901 tgtataagtg tgagctactt caaatttgta ggcttaaata gatccgcccg gtgacaaggt
     3961 gaaacctaaa tctaacattt tcggtgtcac agattcgcca cgaatacgcg ccagcaggcg
     4021 ttcagcgcca atactgccca tccgctcacg cggcgtcagc acgctcgcta accgcggctc
     4081 catcacctgg ccaatgtcat gaccgtggaa accggcaatc gccatatcgt caggaacctt
     4141 caatcccaga cgctgacatt caaacgccgc gccgaccgcc aggtcatcgt tggtacagaa
     4201 cacgccatcc agctgcggat attcccgccg cgcctggcga atcagttcaa taccggaaga
     4261 gtaagaagaa gattgctcaa ccatcacgct atacggcacc agacctgcat ccagcatcgc
     4321 ctgttcgtat cccttctgtt tgatgatagt acgttcgtcg agacgcgcgc cgagataggc
     4381 aatgtggcaa tgcccgcgag caataatggc ggtggtcatc tggcgtgccg cttcaaagtt
     4441 atcaaaaccg acggcgatat cgaggcacgg cgactggctg tccatgagtt ccaccaccgg
     4501 gatccccgcc acttcaatca tctttaaggt gcgcggcgtg tgggtacgtt cggtgaggat
     4561 cagtccgtcg atattccagg agagcatgga ttcgagacgt tcttgctcca tttccggttt
     4621 ataaccgtag tgagccagca tggtctgata accgtgcgcg tcggtgacgc tttcgattcc
     4681 gcgtaatact tccgcgaaaa cctggttggt gagagaaggt aacaggacgc caatcgcccg
     4741 gctggtggcg ttagagagga tatcgggcgc acgattggga atatagccca gttcatcaag
     4801 agcggcggca atcttgccgc gtagagcgac ggaaacctgc tccgggttgc gtaaaaaacg
     4861 gctgaccgtc attttggtca cgcctacacg gtcagccaca tcctgaagta cgggtctttt
     4921 ctttttcatc gtcctgaagg tacaaaagag atagattctc ttagtttaac acggacgcat
     4981 cagaaccttc ccggggaaaa cagtgcttgt ttacaattat tagatataca ctaaatcaat
     5041 tgcatcgcat tgtgctaacg acgcggtaaa accccggaag cgtgcatcag cacgcgaccg
     5101 gagcgagcga ggaagcctgc aaaaaggcag gcttcaaaga tgccgttgag ttttaaaccg
     5161 gcggcagatc gaacagtaac acttcgctgt cgctatccgc atggatggag attgcctgct
     5221 catcccagat tgccagacca tcgctggtcg aggctttcac gccgttaatg gtgacattgc
     5281 ctttcaccac ctggatccag acgcggcgtt cagcggcaat ctgatgcacc gactgctcat
     5341 ctttcagcaa cgcccagcgg tacagttcca tatcctgatg cactttcaat gaaccatctc
     5401 gcgcatccgg cgagagcacc agctgtttgc cctgtacggc atcgaagcga cgctgttcat
     5461 aacgcggcgt aataccgttt tcttcaggca tgatccagat ctgatacaga tgcagacgct
     5521 cggtgctgct tgggttgtac tctgagtgac gaatacccgt acccgcactc ataatctgga
     5581 actcacccgc cggaacctgc tctttattgc ccatgctgtc ctgatgctca acagtacctt
     5641 ccagcacgta ggtcaaaatt tccatatctt tatgcgggtg agtgccgaag ccctgccctg
     5701 cttcaatcac gtcgtcgtta atcacgcgca gcgcggagaa gcccataaag ttcggatcgt
     5761 aatagttggc aaaagagaaa gtatgccagg agtccagcca gccatgattt gcatgaccac
     5821 gttcatttgc tttgcgtaag tagatcattt gtattcgccc catgaatgat ttcgatgggc
     5881 ttagtgtgga cccgatctgc ctgggatgat agagggtgaa aattgacccc tctgttcaaa
     5941 aaaattgaac tattcgaggg gccatcaaac ttacttcgcg agagtcactg tggagggaga
     6001 ggcttgctca aatccgcgtt caaggatttc cagattggta agaacttcag attccttgac
     6061 gtaatttggc gcaccgtggg tgatggtttg atacaacgca tcataaacgc gcccgtaatc
     6121 gcccatctcc ggcttcatct cttctctgac cgtcacgccc tcgtcattga catactccag
     6181 cacaccgacc gaatcatccg ctgcgaatcc cggttcgccc ggcataatat tagccttcag
     6241 gctggtttcc tgctggtcga taccgtattt aataaacgaa cctttcttac cgtgaacgat
     6301 aaatttcgga taatcgattt tcaccagatg gctggttttg acgatggctt tcaggtcgcc
     6361 ataaaacagt tgcgcttcaa aggtgtcgtc aggattggct ttattacgca ggctgcagat
     6421 gtcataagcg acgtgatccg ggcgaccgaa cagagaaata atctggtcca tcgtatgcac
     6481 accaaggcca tagaacgcgc catcctgcgg caacccaggt ttggtttctg ccaccgggcg
     6541 gtaatagtca aaatggcttt ccacttcaac aatctctccc agcttaccgc tttcaatcgc
     6601 ttttttcgcc gtcaggaagc aggaatcaaa gcgacgattc tgatatggcg tgacggtcag
     6661 ccctttgctt ttcgccagtg caaacagctc tttcgcctgc gcaagtgtcg gagtgaacgg
     6721 tttttcgacc agcacatttt tcccggcttc cagcgcgcgc ttcgcgtact cgaagtggct
     6781 gtccgcatgg gtgcagacaa caaccaactt aacatcggga tcgtttagca cttcgtcgag
     6841 atcgctggtg aaatggatat gagagtaaat gggagcctgt tcttccggct tcgcatggcg
     6901 acgaaaaata tgcgcgacat gccagctatc cttgcggtta agtatatacg gcagatggta
     6961 acgggtggtg cttttgccga agccaataaa ggcgcagttg atgaccatga tttcgtcctt
     7021 tttaaggtgg ttatttacac cttagcgcaa agcggacgtg gttcctactg gagtgcgcat
     7081 aacgaacaca agcactcccg tggataaatt gagaacgaaa gatcaaaaaa aaagccagca
     7141 cccggctggc taagtaatac tggaagcaat gtgagcaatg tcgtgctttc aggttctccg
     7201 cgagggtctt cctgatcgcg agacaataat aatcattctc attcgcactt gtccaacact
     7261 ttttgcaaaa aaaatgcatt tgactcgcat ttgaaagtca atgatgttga aagggacatt
     7321 taccccaaag aggacaaagg aatgagtgag atagtaatac gccacgcaga aacacgggat
     7381 tacgaggcca tcaggcagat tcacgcccag ccggaggtgt attgcaacac actacaggtg
     7441 cctcatcctt ccgatcatat gtggcaggag cgactcgccg atcgtcccgg catcaagcaa
     7501 ctcgtcgcct gtattgatgg agacgtcgtg ggccatctca ccattgacgt gcaacagcac
     7561 ccacgccgca gtcatgttgc cgattttggt atctgtgtcg actctcgttg gaagaaccgc
     7621 ggcgtcgcca gcgccctgat gcgagagatg attgaaatgt gcgacaactg gttgcgggta
     7681 gatcgcattg aactaaccgt gtttgtcgat aacgccccgg caattaaggt ctataaaaaa
     7741 tacggctttg aaattgaagg gactggtaag aagtacgcat
//
LOCUS       DAJTVJ010000174         7613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000174 DAJTVJ010000000
VERSION     DAJTVJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.174"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..96
                     /locus_tag="OO448_003867"
     CDS             <1..96
                     /locus_tag="OO448_003867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6338961.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="OO448_003868"
     CDS             complement(152..487)
                     /locus_tag="OO448_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6338962.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="OO448_003869"
     CDS             complement(491..835)
                     /locus_tag="OO448_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6338963.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="OO448_003870"
     CDS             complement(837..1010)
                     /locus_tag="OO448_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338964.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="OO448_003871"
     CDS             1111..1296
                     /locus_tag="OO448_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338965.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="OO448_003872"
     CDS             1257..1376
                     /locus_tag="OO448_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6338966.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO448_003873"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO448_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6338967.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO448_003874"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO448_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6338968.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO448_003875"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO448_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6338969.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="OO448_003876"
     CDS             4176..4544
                     /locus_tag="OO448_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6338970.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="OO448_003877"
     CDS             complement(4652..5053)
                     /locus_tag="OO448_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6338971.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="OO448_003878"
     CDS             5295..5588
                     /locus_tag="OO448_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6338972.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="OO448_003879"
     CDS             5660..6319
                     /locus_tag="OO448_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6338973.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="OO448_003880"
     CDS             6411..6857
                     /locus_tag="OO448_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6338974.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7613)
                     /gene="hlyE"
                     /locus_tag="OO448_003881"
     CDS             complement(7064..>7613)
                     /gene="hlyE"
                     /locus_tag="OO448_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR6338975.1"
                     /translation="DGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSY
                     FQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFT
                     TLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKM
                     INTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1955 a         1710 c         1771 g         2177 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tca
//
LOCUS       DAJTVJ010000175         7611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000175 DAJTVJ010000000
VERSION     DAJTVJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.175"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(81..2459)
                     /gene="ppsA"
                     /locus_tag="OO448_003882"
     CDS             complement(81..2459)
                     /gene="ppsA"
                     /locus_tag="OO448_003882"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6338976.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            2792..3625
                     /gene="ppsR"
                     /locus_tag="OO448_003883"
     CDS             2792..3625
                     /gene="ppsR"
                     /locus_tag="OO448_003883"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6338977.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            3782..4828
                     /gene="aroH"
                     /locus_tag="OO448_003884"
     CDS             3782..4828
                     /gene="aroH"
                     /locus_tag="OO448_003884"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6338978.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            4960..5151
                     /gene="hemP"
                     /locus_tag="OO448_003885"
     CDS             4960..5151
                     /gene="hemP"
                     /locus_tag="OO448_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6338979.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5155..6591)
                     /locus_tag="OO448_003886"
     CDS             complement(5155..6591)
                     /locus_tag="OO448_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6338980.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6654..7367)
                     /gene="ydiV"
                     /locus_tag="OO448_003887"
     CDS             complement(6654..7367)
                     /gene="ydiV"
                     /locus_tag="OO448_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6338981.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         1929 a         2023 c         1808 g         1851 t
ORIGIN      
        1 gatgtctgtg aagagattga ataagtttca tcttcgggga tcacataacc ccggcgactg
       61 aacgccgccg gggatttatt ttatttcttc agttcagcca agcttaacca ggtttgcacc
      121 acggtgtccg ggttcagaga caggctatcg atcccctctt ccatcaacca tgcggcaaag
      181 tcttcgtggt cggacggacc ctgaccgcaa atcccgacat atttgccctg tttcttcgcg
      241 gcacggatag ccatcgacag cagtgctttc accgcatcgt tgcgctcatc gaacaattca
      301 gacaccacgc cggagtcacg gtccagaccg agcgccagct gcgtcatatc gtttgagcca
      361 attgagaagc cgtcgaaata ttcgaggaac tgctcggcca gcaaggcgtt ggacgggatt
      421 tcacacatca tgatgatttt cagcccgttc tcaccacgct tcagcccctg acgcgccagt
      481 tcctcaacca ccgctttcgc ctgatctacg gtacgcacga acgggatcat gatctcaacg
      541 ttggtcagcc ccatgtcgtt gcgcacacgt ttcactgctt cgcactccag cgcgaagcag
      601 tcgcggaagc tgtcggaaac atagcgtccc gcgccacgga agccgagcat cgggttctct
      661 tcatctggct cgtaacgctc accaccgacc aggttggcat attcgttcga tttaaaatca
      721 gagagacgga caatgacgcg cttcggataa aacgcggcac ccagcgtcgc gatcccttca
      781 gtcagacgac caacgtaaaa ttcacgcgga gaatcaaaac ctttcatcat ctcgcggatt
      841 tcattttgca actgcggttc ctgatcgtca aactcaagca gtgcgcgtgg gtggacgcca
      901 atcatacggt tgatgataaa ttccagacgc gcaaggccca cgccttcgtt cggcaggcag
      961 gcgaagtcga aagcacggtc cgggttaccg acgttcatca tcactttcaa cggcagatcc
     1021 ggcatcattt ctacgctgga gcttttcacg ctaaattcca gcaactccgc atagacgtaa
     1081 ccggtatcac cttcggcaca agaaacagtg acgttctcac cgtctttcat ccgttctgtt
     1141 gcatcaccac agcccactac cgccggaatg cccagttcac gagcgatgat cgccgcgtga
     1201 caggtacgac cgccacggtt ggtgacgatg gcagatgctt tcttcatgat cggttcccag
     1261 tccgggtcgg tcatgtcagt gaccagcacg tcaccaggtt cgatgcggtt catttcgctg
     1321 atctcatgga tgactttcac cggacccgcg ccgatgcgat gaccgatagc acggccttcg
     1381 gcgataatct taccctgtga atgcagcgta taacgctcca tgacctgacc gcgtgagcgc
     1441 acggtttccg gacgcgcctg cacaatgaac agtttgccag tgtggccatc tttcgcccac
     1501 tcaatatcca tcgggcgacc gtagtgtttc tcaatttgta cggcctgttt tgccagttcc
     1561 tgcacttctt cgttggtcag cgagaagatg tcacgctgtt cctgcggtac gtcttcgatt
     1621 ttaacctgct tgccgtgctc ctgggtcggc gcgtaaacca tgcggatttt tttcgacccc
     1681 atggtgcgac gcacgatagc cgggcgattc gccgccagtg tcggtttatg gacgtaaaac
     1741 tcatccgggt taaccgcacc ctgcacgacc atttccccaa ggccccatgc ggaagtgata
     1801 aacaccacct ggtcaaagcc ggattcggta tcaatggaga acatcacgcc agaagatgcg
     1861 aggtcggagc gcaccatccg ctgaacaccg gcagagagcg ccacgccacg gtgatcgtaa
     1921 ccctggtgca cacgataaga gatggcgcga tcgttaaaca gagaagcaaa tacatgcttc
     1981 actgccacga gaacggcgtc aaaaccctga acgttgagga aggtttcctg ctgaccggca
     2041 aaagaagcgt ccggcatatc ttctgcggtg gcggaggagc gcaccgcaaa agaggcgttt
     2101 tcgtcatcgg cggaaagctg tgcataggct tcgcggatgg cgttttccag ctcaggctgg
     2161 aagggagtgt cgataatcca ctggcggatt tgcgcgcccg ctttcgcaag ctgagtaaca
     2221 tcgtcaatat ccgttttatc cagcagttca taaatgcgct ggtttacgcc gctttggtcc
     2281 agaaactggt taaacgcgtc ggcggttgtg gcgaaaccat tcggaacgga aacacccatt
     2341 ccggaaaggt tagtaatcat ttcacccagg gaggcatttt tgcccccaac cctgtctaca
     2401 tcattcatgc cgagttggtt ataccaaagc accagcggtg acgagccatt gttggacatc
     2461 gaacaatcct tttgtgataa atgaacggtt tgagaaacac atttctgcgc atttatcttt
     2521 gcatatttaa ccggatgaaa aaaacggtga atcgttcaag caaatatatt tttttacttt
     2581 ttaagactga tcccagcgtt gcgcaaatct gctctcttcg acaatttcca cataaaccat
     2641 cggtataaac gaaaccataa aaatgaaatg ctgttttcat aaaaaataaa attgaaggtt
     2701 cattttataa accagacata acgtcctacg cttctgtgcg tttttaattt atgctttcat
     2761 agaattatgt ctgcatcacg ggaagaacaa aatggataat gctgttgatc gccacgtttt
     2821 ttatatttct gatggtacgg caataactgc ggaggtatta ggacacgcag taatgtcaca
     2881 atttcccgtc actatcagca gcattacgct gccgtttgtc gaaaatgaga gccgtgcacg
     2941 ggcagtgaag gatcagattg acgcaattta tcaccagaca ggtgtgcgcc cgctggtctt
     3001 ctactccatc gtgttgccgg agattcgcgc catcatcttg caaagtgaag gcttttgcca
     3061 ggatatcgtt caggcgctgg ttgccccgct acaacaagag atgaaactgg atccaacgcc
     3121 gattgctcat cgtacccatg gccttaaccc taataatctc aataaatatg atgcgcgcat
     3181 tgcggcgatt gattacaccc tcgcccacga tgacggcatt tcgttgcgca atctggatca
     3241 ggctcaggtg atcctgctcg gtgtttctcg ctgtggtaaa acccccacca gtctttatct
     3301 ggcaatgcaa tttggtatcc gcgcggcaaa ctaccccttt attgccgacg atatggataa
     3361 tctggtgcta cccgcgtcgc tcaaaccgct tcagcataaa ttgttcggcc tgactatcga
     3421 cccggaacgt ctggcggcga ttcgcgagga acgtcgggag aacagtcgct atgcctcgct
     3481 tcgtcagtgc aggatggaag tcgcggaagt ggaagcgttg taccgtaaaa atcagatccc
     3541 gtggattaac agtaccaatt attcggtaga agagattgcc accaagatcc tcgatatcat
     3601 gggccttagt cgccgaatgt actagagaac tagtgcatta gcttattttt ttgttatgat
     3661 gctaaccacc cggcgaggtg tgacacacct cgcacttgaa atcagcagcg attggtttat
     3721 cgtgatgcgc atcacttccc ggcagtcctg ccgtagaagc aacaaatttc tgagacttgt
     3781 aatgaacaga actgacgaac tccgtactgc gcgtattgag agcctggtaa cgcccgccga
     3841 actcgcgcta cggtatcccg taacgcctgg cgccgccacc catgtcaccg actcccgccg
     3901 cagaattgaa aaaatactca atggtgaaga taagcgactg ttggtcatta ttggcccctg
     3961 ctcgatccac gatctcaccg ctgcaatgga gtacgccccc cgtctgcagt cgctgcgcaa
     4021 ccagtaccag tcacggctgg aaatcgtaat gcgcacctat tttgaaaaac cacgaactgt
     4081 tgtcggctgg aaaggactaa tctccgatcc agatttaaac ggcagctatc gggtaaatca
     4141 cggtctggag ctggcgcgca aattactttt acaggtaaat gagctgggcg tcccaaccgc
     4201 gactgagttc ctcgatatgg tgaccggtca gtttattgct gatttaatca gttggggcgc
     4261 gattggcgca cgtactaccg aaagtcagat ccaccgtgaa atggcttcgg cactctcctg
     4321 tccggtaggt tttaaaaatg gtaccgatgg caatacgcgg attgctgtgg atgctatccg
     4381 cgcagcccgc gccagccata tgttcctctc gccagacaaa aatggtcaga tgaccatcta
     4441 tcagaccagc ggcaacccgt atggccacat tattatgcgt ggcggcaaaa aaccgaatta
     4501 tcatgccgat gatatcgccg cagcctgcga tacgctgcac gagtttgatt tacctgaaca
     4561 tctggtggtg gatttcagcc acggtaactg ccagaagcag caccgtcgcc agttagaagt
     4621 ttgtgaggat atttgtcagc aaatccgcaa tggctctacg gcgattgctg gaattatggc
     4681 ggaaagtttc ctgctcgaag gaacgcaaaa aatcgtcggc agtcagccgc tcacttacgg
     4741 tcaatccatt accgacccgt gtctgggctg ggaagatacc gaacgcctgg tcgaaaaact
     4801 cgcctctgcg gtagataccc gcttctgaat gcgtgcccat tcctgacgga atgggcattt
     4861 ctgcgcaact tgttgtcttc tcaacaaatt actgcttgct ctggcctgcc ataatattga
     4921 taataagaat cattgttata tcaattatta ttaatcttta tgcgttatac ggatagcaga
     4981 aaactcacgc ctgaaacgga cgccaatcac aaggccgctt ccccgcagcc catccggcga
     5041 attaccagcc agacactgtt aggtccggat ggcaaactga ttatcgatca tgacgggcaa
     5101 gaatatctgc tccgtaaaac tcaggctggc aagctgctgt taactaagta gcctttaact
     5161 cgagcagctg acttccagcc gtttacccca gtcaggtgga cgactgacat agtcatcatc
     5221 tctatcgctg aaaggatttc gcaacgcccc atgcaggcgg tgcaattccg tcatatcacc
     5281 cttttctgcc gcttcaatcg cccgttgcgc caaccagttg cgcaatacca gagcggggtt
     5341 aacgctttgc atcaactgct gacgctcact atcgctaacc tcgtcttgtt gcaaacgccc
     5401 ccgataacgg gcaaaccagc catcaaatgc cgcacgatca ataaactcat cacgtagcgg
     5461 tgacgccgtg ctgtgctgct cggtcagact cagcatgcgg aatgtgcggg tataatcgct
     5521 gcgctctcgc gccatcagac tgaataattc attcagtagc gcgttatcct ctttttgctc
     5581 cgtgatgaag cccagtttct gccgcatccg ttgtccataa tgcgtcaaca aaacctgctg
     5641 atagctgtcc agggcctcat tcagggcatc tacggcaaca aatggtgaca atgtctgcgc
     5701 cagacgctgt aaattccaca acgcgacagc aggttgatta tcaaaactat aacgcccttg
     5761 atgatccgag tgattacaaa taaaaccggg ttcgtaatca tcaagaaaac caaacggccc
     5821 gtaatcaagc gtcagtccca gcagcgacat gttgtcggta ttcatcaccc catgtgcaaa
     5881 gccgaccgtc tgccattggg caattaacga cgcggtacgt gcgacaacat cgctaaacca
     5941 gagacggtat ttgtcctcat catcttcaag atgtgaccag taatgacgaa tagcaaagtc
     6001 agctaactga cgaacctttt ccgactcgcg gcggtagtaa aaatgttcga aatgaccaaa
     6061 gcgcagatga cttggtgcca cacgcatcag catcgcgcct ggctccgccg tttcccgata
     6121 cactggcgaa tcgctggtga cgatacttaa cgcgcgggtc gtcggaatgc ccagataatg
     6181 catcgcctca ctggcgagac tttctcgtat cgtcgaacgt aaaaccgccc gtccatcacc
     6241 cattcgcgaa taaggcgtca ggccagcacc tttcagatgc cagtccattg tagtgccatc
     6301 agcaagcagt tgttcgccga gtaaaatgcc gcgcccatca cccagttggc ccgcccagac
     6361 gccgaactga tgaccactgt aaacctgcgc cagtggtgac ataccaggca gtagggcttc
     6421 gccgccccag acacctgcgc catttttaaa cagcgacgat ggaatactca gcgtgttagc
     6481 cagttcggta ttatgccaaa tcagccgggc attatttaaa ggcgtagggg aaagtgctgt
     6541 ataggtttct ggcaattcat cgcgccagcg ggtaacaaaa gacagggtca tagatcctcc
     6601 tgtttgatag tgtagacggt tactctcgtc ttaaacacca gcaaacagaa gggttatcgc
     6661 tgaaccaacg tcgttatctg atttatggga acggcaggcc acaggcatcc ctggagagca
     6721 tggaaatcga agggggtaat ctgtgccaat atttctgccg tatcaatgcc tcccgcaatg
     6781 atgcaattgc aacaagggga aatttgcgcc tggatcgcac gaataaacgg ttcgaaggag
     6841 cggtgcgtga tttgttgctg aataaaactt ttatccagca tgacgcgggt gaaaagacca
     6901 tcaaaaaccg ctttcattgt gctattgcct gctcccaaat tacctaacac aaggggatac
     6961 acttgcgata aggataaaag accgcgatta tcttttcctt cattgagatg cgggtaattc
     7021 tcattaatca acagttcaat aaacggatat tttaatagtt cgcctgcgta attatccctc
     7081 tctaataata atgtagccac ttgtggcgtt aaattaagcc aggcaaagag tttatgttga
     7141 ataaaaaaat gttggcatga ttttagtaat tccaactgct ctgaaaataa ttgccaatgt
     7201 tgctcctcgg taagttgcgc tataacccga ctcgtcggga tacgcaccgt gccatcttca
     7261 ctggagaaat gggtaattaa ctcaacgccg acaaggactt gctggttatc tcttatcggc
     7321 aggaaatagc aatcagaatg ataaagattc tccaaaaaaa tcttcatggt aaccgtccct
     7381 ctcgaaggat gtttttcagt atccggctaa aagggatgag gccataagac ataacaatta
     7441 cagaaggagt aactttcatt tgttccatgt taaccacttt ttcaggggtc ctttttttag
     7501 attatcctga ttataaacga ataatccatt tacggaattt ttgtctgcaa aatactactg
     7561 tattcaggag taaatggacg gcgatgtatc atcgccgtcc atttttcgac a
//
LOCUS       DAJTVJ010000176         7586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000176 DAJTVJ010000000
VERSION     DAJTVJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.176"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..183)
                     /locus_tag="OO448_003888"
     CDS             complement(<1..183)
                     /locus_tag="OO448_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338982.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLT"
     gene            complement(1004..1216)
                     /locus_tag="OO448_003889"
     CDS             complement(1004..1216)
                     /locus_tag="OO448_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCR6338983.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1292..1909)
                     /locus_tag="OO448_003890"
     CDS             complement(1292..1909)
                     /locus_tag="OO448_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCR6338984.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2176..3675
                     /gene="ansP"
                     /locus_tag="OO448_003891"
     CDS             2176..3675
                     /gene="ansP"
                     /locus_tag="OO448_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR6338985.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3790..4851)
                     /locus_tag="OO448_003892"
     CDS             complement(3790..4851)
                     /locus_tag="OO448_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR6338986.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5093..7195
                     /locus_tag="OO448_003893"
     CDS             5093..7195
                     /locus_tag="OO448_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6338987.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7231..>7586)
                     /gene="mcbR"
                     /locus_tag="OO448_003894"
     CDS             complement(7231..>7586)
                     /gene="mcbR"
                     /locus_tag="OO448_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6338988.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
BASE COUNT         1943 a         1757 c         1830 g         2056 t
ORIGIN      
        1 cgtcaggtag gccattttgt ccagcacctg cgcaaattca agggagagaa actgctggct
       61 gaccagagag agatcgaggg aaaagagcga agagagtgac tggttcaaat gaaaggaaac
      121 caccgcaaaa gcatccggtg gcaggccgtc cacttccagt gtgaaacgta atccggttga
      181 catagcaacc tccgtgaaaa tgaataataa tccgcacata ttgcatgtac aacatgtcgg
      241 gcggatgaga taagcgagtc gacatctggc gttaccgggg aatatcagaa aaatgtcatg
      301 gttatgtgat gatttagtct gggattttac ttcagagaaa cgggtgtctg tttagtcaac
      361 aaaataatca caaacgcaac ggatgggttc tcgacgttat tcacgacagg ctcgtacatt
      421 acagcgagta ataaaaaacc tgatatgcaa agtcaaacca atattcataa tgtcttttta
      481 ttcacaacaa taaataattc aataacaatc attgaaatga atgatggttt gtataaggaa
      541 tatacgctac cgcccaccct gataacatga tctgcacatt gccatcttaa cgtcgttaaa
      601 cactggctta atagtggata tttcaaatta ggaatttatt aaattcacct gatgaatata
      661 ataagcaaat agcatagaaa ataatggcaa ctccccccga gaggaccatt tccaaagcac
      721 tgcaccacca cctggtgccc ggatgatctt gcttcagtgg taagagtacc tgattcattg
      781 ttcccaaaat gatgttttcc ccggccccgc caataatgtg cagctctcac gtgataatct
      841 attgattagg ttatattaag aatcaatatt taaaatacat ccattttctt gaataaatca
      901 acatatttat ttatcaattg ctgttttagc tctgactcat caagctctcc atatttatcc
      961 tatagataat tgcgaataaa tacagagaac gccatttatc cttttagcat acctgccgga
     1021 tatttttatt tgcattaata ttaattgcat ctccaactcc gagcatatcc atcattaaaa
     1081 actttttgag attatagaat acctgtgata acaagtgaaa catctgcaca atgtgaatag
     1141 ttagtttctt ttcagaggaa atgaaataga caagctgact gaaggggaga gtgatataaa
     1201 tcaaaaaaca taacatgaat ttaattaatt acgcccatca ttgtggataa agttactgaa
     1261 taataaataa cataatcctc tggtatccca ctcaaataat ttcattcctt tttaatactt
     1321 cttgtaattt cggtaactga catacagcat cagcaatcgc actgatattc gtggcattat
     1381 cctgaaacca ttcatgccca gacccccatg tgcgcatagt gcaaagataa cagtccacca
     1441 gcgttagttg ctcaccaaac gcataaggtt cagcggtcaa ttgtgaattc agccagatat
     1501 aaagcgattt acgatattca ataacattct tctttaactg ttcaggtgcg tcaggagccc
     1561 aacgctcagg gtaatcggcg aaagtgaatg ttggatagac attagcaacc agccagacca
     1621 atagccgttg aaactgctga cgttcggcgc gcccaaccgg tggcgcaagg tccgggcgac
     1681 gatcgagaac catcaatgca atcgccgctg tctccgtcat aatctcgtca ttttccagcg
     1741 ccagtgtcgg aatctgacac aacgggttca gggttttcaa taactcgcgt gaagttccct
     1801 cgtggtcaaa accactgaca tcaacgaact gataaggaat atcagccagt gttagcatca
     1861 gctcactgat tgtagagccc catccgggta cgccatacac cttaatcata tgcccctcac
     1921 aatcttttaa agattaagtg taggacagta cacaggttaa ccagcaaaag tatttgtcat
     1981 tttttcgtca gttgtatgaa tccccttcag gattgtaagg ggatgttttg catcgtatat
     2041 cgataacggg taggatgctg cgccgatagt tttataccca taaagaataa tggtgataac
     2101 tatcatcgcc aggatgaata aacattgttc atggcaactt atatgacttt ttcattaaag
     2161 caatcaggga gagcaatgag taaacacgac accgacactt cagatcaaca cgccgcgaaa
     2221 cgccgctggc ttaatgccca cgaagagggg tatcacaaag cgatgggcaa tcgccaggta
     2281 cagatgatcg ccattggcgg cgcgattggc accggcttgt ttttaggtgc aggagcccga
     2341 ctgcaaatgg cgggtccagc actggcactg gtttatttaa tttgtggctt gttttcgttt
     2401 tttattctgc gtgcattggg tgagctggtg ctacaccgcc cttccagtgg cagttttgtc
     2461 tcttatgccc gtgagttttt gggtgagaaa gccgcttatg ttgctggctg gatgtacttc
     2521 atcaactggg cgatgacggg gattgttgat attaccgccg tcgctctgta tatgcattac
     2581 tggggtgcgt ttggcggcgt gccgcagtgg gtctttgcgc tcgctgcact taccatcgtt
     2641 ggcaccatga atatgatcgg tgtgaaatgg tttgcggaga tggagttctg gtttgcgctt
     2701 attaaagtgc tcgccattgt gacctttttg gtcgtgggta cagtgttcct cggtagtggt
     2761 cagccgctgg atggcaacac cactggcttt catttaatca ccgataatgg cggcttcttc
     2821 ccccacggtt tgctgcctgc gctggtgttg attcagggcg tagtgtttgc ttttgcctcc
     2881 attgaaatgg tgggtacagc tgccggagaa tgtaaagatc cgcagaccat ggtgcctaaa
     2941 gccattaaca gtgtgatttg gcgtattggc ctgttttacg tcggctccgt ggtgttgctg
     3001 gttatgttat tgccgtggag cgcgtatcag gcggggcaaa gtccgttcgt gacgtttttc
     3061 tctaaactgg gtgtgccata tatcggcagc attatgaaca ttgtggtgct gaccgctgcc
     3121 ctctccagcc tgaactcagg tctgtactgc accgggcgta ttctgcgctc gatggcgatg
     3181 ggcggttccg caccgagttt tatggcgaaa atgagtcgtc agcatgtgcc gtatgccggg
     3241 attctggcga cactggttgt gtatgtcgtc ggcgtattcc tcaactatct ggtgccgtcg
     3301 cgcgtatttg agattgtgtt gaacttcgcg tcgctgggaa tcatcgcttc atgggcgttt
     3361 atcatcgtgt gccagatgcg cctgcgtaaa gcgattaaag aaggcaaagc agcggatgtc
     3421 agttttaaac tgcctggcgc gcccttcact tcctggctga cattactgtt tttactgagt
     3481 gtccttgtgc tgatggcgtt cgattacccg aacgggactt atactatcgc ggcgctgccg
     3541 attatcggta ttctgctggt tataggctgg tttggtgtgc gcaaacgcgt tgctgaaatt
     3601 cacagcactg cgccagtcgt cgaggaagat gaagaaaaac aggaaattgt gtttaagcct
     3661 gaaacggcga gctaattgca aaattgcccg gctacgtgcc gggcaaattt agattactga
     3721 cgaaggtgtg ccgcatgcgg cgtgataaaa gtcaggcaag tctctccgtt ttcaccctcg
     3781 ccatatctat tacagcgcaa tacgaatcac atcgtctggc tgggtagctt cctgctgttt
     3841 agtggatttt tgtttcacac tgacatacag cgttttgcca tcggcagaca gcgccaggct
     3901 gtttggatga gtcggcgtat cgaacgtttt cactacttta tagcttttcg cgtcaatcac
     3961 actgacttta cctgcctgac gatgcgttac gtaggcttca ttacgcgctg ggttaaacag
     4021 cacagccagt gattccggtg ctgcaacctt cgccagaata ttgccattac gggtatcgac
     4081 cactaacact tctgcggctt tagaatcggt gataaatgca cgctgcctag cggtatcaag
     4141 gctaatgttg ataaagaagt gctctttgcc gtcatccagc agctttttac ggctgaggat
     4201 tttattgtcg gcggtgtcga tggtaatcaa ttcgccgtca gcgttagtgg tgtaaagacg
     4261 tttgcctttg ctatcaagcg ccagaccggt actcatttta ccggtgttct ggatggcggt
     4321 tttcagtttg atattctcgc catcaacgac ccaaatcacg ctctctttac caataccact
     4381 gatgtaaacg gtgttcgtgg catcgtcagc taccagctca cgcggttgca gcgggcgcac
     4441 ctcttccgta cgcttacgat catccagcac cagacggcct ttcacctcgc ccgttttggc
     4501 atctatcgcc gtgaccgcgc tgtttacagt gttaccaaac cacagtgtct gagtcgtgtt
     4561 attgatggtg acaccaaacg gcttgagatc gttatggatc gcctgcgtca cttccagagt
     4621 gaccggatca agacgataaa ccacgccgcc tttatccagt ttgcggcttt gcgaagtggc
     4681 gagccacagc gcgttttctt gctggctata agccatttcg taggcacctt tacctaccgc
     4741 tttacgcagc atttcttctg cggcctgcgt actgaatgat gaagcaacaa gcaatgaacc
     4801 taacagtaat gaaccacgca ggcgcgatga aaacagatga cgtaaatgca tgacgactcc
     4861 ctttgatgaa ccgatatttt tgctgcttca caatgcttcg ctaagcgtaa tcatcgttac
     4921 gctcttgtta taaatgggaa tggtaatcat tattttctca aatgtggagc atttcttgcc
     4981 gttacgacta caattttctc aattacttaa cgtatcgtgc cgtttcgttt tccatgaatt
     5041 tacaatttta ttaccataac ggcgttttcc cattcttaac ctggcttttt tcatgaagat
     5101 tttttccgtc cgacagaccg ttttgcccgc actacttgcc ctttcccccg ttgtttttgc
     5161 cgctgatgaa cagaccatga ttgtcagtgc cgcaccgcag gtggtttcag aactggatac
     5221 cccagcagca gtaagcgtgg tggatggcga ggagatgcgc ctggcaacac cgcgcattaa
     5281 cttgtccgaa tcactgaccg acgtgcctgg tttgcaggta caaaaccggc agaactatgc
     5341 gcaagattta cagctgtcga ttcgcggatt tggctcccgc tccacttacg gtattcgcgg
     5401 tattcgcctg tatgtggacg gtattcccgc caccatgccc gacgggcaag ggcaaacatc
     5461 caacatcgat ttaagcagtg tgcaaaatgt ggaagtgctg cgtggcccct tctctgccct
     5521 gtatggcaac gcgtctggtg gggtaatgaa tgtcaccacc cagaccggac aacagccacc
     5581 aaccattgaa gccagtagtt actacggcag ttttggcagc tggcgctatg ggctgaaagc
     5641 aacgggcgca acgggagacg gcacacagcc tggcgatgtc gattacaccg tctcaaccac
     5701 gcgttttacg acccacggct atcgtgacca tagtggcgca cagaaaaatt tagccaatgc
     5761 caaactgggc gtacgcattg atgaagccag caaattaagt ctgattttca atagtgtgga
     5821 tatcaaagca gatgacccgg gtgggctaac caaagcagaa tggaaggcta atccacaaca
     5881 agcgcctcgt gcagaacagt acgacacgcg aaaaaccatc aagcaaactc aggctgggtt
     5941 gcgctatgag cgtagcctga gcgcgcaaga tgatatgagt gtgatgatgt atgccggaga
     6001 gcgagaaacg acccagtacc agtcaatacc catggcacca caacttaacc cgtcacatgc
     6061 gggcggcgtg attaccctgc aacgccatta ccaggggata gacagccgct ggacacaccg
     6121 tggtgaactg ggcgttccgg tcacgttcac taccggcctg aactacgaaa acatgagtga
     6181 aaaccgcaag ggctacaata acttccgcct gaatagcggc atgccggagt acgggcaaaa
     6241 aggtgagttg cgtcgcgacg aacgcaatct gatgtggaac atcgatccct atttacagac
     6301 gcagtggcag ctgagcgaaa aactgtcgct ggatgctggc gtgcgctaca gctccgtgtg
     6361 gtttgattcc aacgaccatt acgttactcc gggtaacggc gatgacagcg gtgatgccag
     6421 ttatcataaa tggctacctg ccggttcgtt aaaatatgca atgaccgatg cctggaatat
     6481 ctatctggca gccgggcgag gttttgaaac gccgacgatt aatgagctgt cttatcgtgc
     6541 tgatgggcaa agcggtatga actttggttt aaaaccatcc accaacgata caattgagat
     6601 cggcagtaaa acgcgtattg gtgatgggct gcttagtctc gcattgtttc agaccgacac
     6661 tgatgatgaa attgttgtcg atagcagtag cggtgggcgt acgacttaca aaaatgccgg
     6721 aaagacccgt cgtcaaggcg ctgaactggc atgggatcaa cgtttcgcag gagattttcg
     6781 cgtaaacgcg tcctggacct ggcttgatgc gacctatcgc agcaatgttt gcaatgaaca
     6841 ggattgtaac ggtaatcgga tgccagggat cgcccgtaat atgggctttg cgtcgatagg
     6901 ttatgtaccg gaagatggtt ggtatgcagg cacggaagcg cgttatatgg gcgatattat
     6961 ggcagatgat gaaaatacgg caaaagcgcc gtcttatact ctcgtcggct tattcaccgg
     7021 gtataaatac aattaccaca atttaactgt ggatttattt ggtcgtgtcg ataatttatt
     7081 cgataaagaa tacgttggtt ctgtcattgt caatgagtca aacgggcgat attacgaacc
     7141 ttcgcccgga cgaaattatg gtgtcggcat gaatattgcg tggagatttg agtaattacc
     7201 ttgatgcccg gtatttgccg ggcatttact ttaacgattg tattgctggt ataaaatagc
     7261 aatattttgt tgcataattt cagcaagaca atgtctgcat ccctctttgt cttttgcttt
     7321 taacgcagcg agtaacagat gatagttttc tatatgctcc cgtaattccg ctggattaat
     7381 cgcttcatag agataatgta aaccaggccc catcctgacc cacagttgct caatcatctc
     7441 acacaggatg ggcatatttg agcgatgata aatcgctaag cgaaatagcc tgtttacatt
     7501 aatgatttgt tccatgtcgc ccttttcttg cgcctgctgt aatttctcga gcaattcctg
     7561 gagttccgtc aggtcttgcg gggtga
//
LOCUS       DAJTVJ010000177         7460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000177 DAJTVJ010000000
VERSION     DAJTVJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.177"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..388
                     /locus_tag="OO448_003895"
     CDS             <1..388
                     /locus_tag="OO448_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6338989.1"
                     /translation="TLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDY
                     ANELHITESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDP
                     AYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            639..2201
                     /gene="hpaB"
                     /locus_tag="OO448_003896"
     CDS             639..2201
                     /gene="hpaB"
                     /locus_tag="OO448_003896"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR6338990.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2219..2731
                     /locus_tag="OO448_003897"
     CDS             2219..2731
                     /locus_tag="OO448_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6338991.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3124..5274
                     /gene="btsT"
                     /locus_tag="OO448_003898"
     CDS             3124..5274
                     /gene="btsT"
                     /locus_tag="OO448_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6338992.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5324..5527
                     /locus_tag="OO448_003899"
     CDS             5324..5527
                     /locus_tag="OO448_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6338993.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5538..6494
                     /gene="yjiA"
                     /locus_tag="OO448_003900"
     CDS             5538..6494
                     /gene="yjiA"
                     /locus_tag="OO448_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6338994.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6572..>7460)
                     /locus_tag="OO448_003901"
     CDS             complement(6572..>7460)
                     /locus_tag="OO448_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6338995.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1728 a         2035 c         1943 g         1754 t
ORIGIN      
        1 caccctactg ctgcgtaatg caaaactcga cgaccatgcc gccagcggga tgcgcggaga
       61 attaaaactg ttccagcgtt ttaatatgct gattgaaagc cattttcatc agcactggac
      121 agtaccggat tacgctaacg aactgcatat caccgaatca cgcctcacgg acatctgccg
      181 ccgctttgcc aaccgtccgc caaaacggtt gatttttgac aggcagctgc gagaagccaa
      241 gcggctgctg ctgttttctg ataacgccgt gaacaatatt gcctggcaac tcggttttaa
      301 ggatccagct tattttgcgc gcttttttaa tcgcttagtc ggttgctcgc ccagtgctta
      361 tcgtgccaaa aaagtacctg tgacgtgaaa atcccttagc cttaacggga aaccaggcac
      421 cacctgctat tcccctttct ccgcctggag caaggggatt tagtaacgtc aaaaatcgct
      481 aaaagcgacc gcgatcacaa aaccgtaccc gacctgaaaa gtaccagcca ttgtcccaaa
      541 agtgtcttcc cgtaatcgcc cctgcaagct tcaattgaat aacaaaaaaa acacacaaaa
      601 ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg
      661 ccagtaccca acgtccgttc accggggaag agtatctgaa aagcctgcag gatggtcgcg
      721 agatctatat ctatggcgag cgagtgaaag acgtcactac tcatccggca tttcgtaatg
      781 cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg caggactctc
      841 tgtgctggaa caccgacacc ggcagcggcg gctataccca taaattcttc cgcgtggcga
      901 aaagtgccga cgacctgcgc cagcaacgtg acgccatcgc tgagtggtca cgcctgagct
      961 atggctggat gggccgtacc ccagactaca aagctgcttt cggttgcgca ctgggcgcga
     1021 atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtacacc cgtattcagg
     1081 aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgatcgt catttgccga
     1141 ccgataaagt gaaagacgtt tacatcaagc tggaaaaaga gactgacgcc gggattatcg
     1201 ttagcggtgc gaaagtggtt gccaccaact cggcgctgac tcactacaac atgattggct
     1261 tcggctcggc acaagtaatg ggcgaaaacc cggacttcgc actgatgttc gttgcgccaa
     1321 tggatgccaa tggcgtcaaa ttaatctccc gcgcctctta tgagatggtc gcgggtgcta
     1381 ccggctcgcc gtacgactac ccgctctcca gccgcttcga tgagaacgat gcgattctgg
     1441 tgatggataa cgtgctgatc ccatgggaaa acgtactgat ctaccgcgat ttcgatcgct
     1501 gccgtcgctg gacaatggaa ggcggttttg cccgtatgta tccgctgcaa gcctgtgtgc
     1561 gcctggcagt gaaactcgac ttcattacag cactgctgaa aaaatcactc gaatgtaccg
     1621 gcaccctgga gttccgtggt gtacaggccg atctcggtga agtggtggcg tggcgcaaca
     1681 ccttctgggc attgagtgac tcgatgtgtt ctgaagcgac gccgtgggtc aacggggctt
     1741 atttaccgga tcatgccaca ctgcaaacct atcgcgtact ggcaccaatg gcctacgcga
     1801 agatcaaaaa cattatcgaa cgcaacgtta ccagtggcct gatctatctc ccttccagtg
     1861 cccgtgacct gaacaatccg cagatcgacc agtatctggc gaagtatgtg cgcggttcga
     1921 atggtatgga tcacgtccag cgcatcaaga tcctcaaact gatgtgggac gccattggca
     1981 gcgagtttgg tggtcgccac gaactgtatg aaatcaacta ctccggtagc caggatgaga
     2041 ttcgcctaca gtgtctgcgc caggcacaaa gctccggcaa tatggacaag atgatggcga
     2101 tggttgatcg ctgcctgtcg gaatacgacc agaacggctg gactgtgccg cacttgcaca
     2161 acaacgacga tatcaacatg ctggataagc tgctgaaata acgcagcagg aggttaagat
     2221 gcaattagat gaacagcgcc tgcgctttcg tgacgcgatg gccagcctgt cggcagcggt
     2281 aaatattatc accaccgagg gcgacgccgg acaatgcggg attacggcaa cggccgtctg
     2341 ctcggtcacg gatacaccac catcgctgat ggtgtgcatt aacgccaaca gtgcgatgaa
     2401 cccggttttt cagggcaacg gtaagttgtg cgtcaacgtc ctcaaccatg agcaggaact
     2461 gatggcacgc cacttcgcgg gcatgacagg catggcgatg gaagagcgtt ttagcctctc
     2521 atgctggcaa aaaggtccgc tggcgcagcc ggtgctgaaa ggttcgctgg ccagtcttga
     2581 aggtgagatc cgcgatgtgc aggcaattgg cacacatctg gtgtatctgg tggagattaa
     2641 aaacatcatc ctcagtgcag aaggtcacgg acttatctac tttaaacgcc gtttccatcc
     2701 ggtgatgctg gaaatggaag ctgcgattta agctccagcc ccttcacgca ttcgtcgaag
     2761 gggcattttt attgtgatct caatcacata tccacccgat gaataattct tgcccaaccg
     2821 ctcaccgcca ttcctgaccg cttacgtccc taaaataacc actcagttat ttaccttact
     2881 ttacgcgcgc gtaactctgg caacatcact gcaggatagc ggtcaattta cctcctcaaa
     2941 cgcaacgcaa acctagaacg gcttcggcct atgattaaca aattactcta tgaattttgg
     3001 tttgcaggaa ggcagcaagt gagtgaatcc ccgggagctt acaacagtaa gtgacagggg
     3061 tgaacgaacg caactgccgc acctgtaagc caaaagacga cgagtaaaat gccaggtttt
     3121 actatggata cgaaaaaact attcaagcac ataccctggg tgattctcgg aatcatcggt
     3181 gcattctgcc tcgcggtagt tgcattacgt cggggggagc acgtcagcgc cctgtggatc
     3241 gtggtcgcct ctgtgtcggt gtatctggtg gcgtatcgct actacagtct gtacatcgcc
     3301 cagaaggtga tgaaactcga ccccacgcgc gcgacgcctg cggttattaa caacgacggt
     3361 ctgaactacg ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc cgctatcgcc
     3421 ggtgctggtc cgctggtcgg tccggttctc gccgcacaga tgggccacct gcctggcacg
     3481 ctgtggctgc tggcgggggt agtactggcc ggtgcggttc aggactttat ggtgctgttt
     3541 atctcctctc gccgtaacgg cgcatctctc ggtgagatga taaaagaaga gatgggacca
     3601 gtaccgggga ctatcgcgct gtttggctgt ttcttaatca tgatcatcat ccttgccgtc
     3661 ctggcgctga ttgtggttaa agccctggcc gaaagtccgt ggggtgtctt caccgtttgc
     3721 tcaaccgtac cgattgcgct gtttatgggt atctacatgc gctttatccg tccggggcgt
     3781 gtgggtgaag tctctgtcat tggtatcgtg ctgctggttg cctctatcta cttcggtggc
     3841 gtgattgctc acgatccgta ctggggtccg gcactgacct ttaaagacac caccattacc
     3901 ttcgcgctga ttggctatgc gtttgtttcc gcattgctgc cggtgtggct gatcctcgca
     3961 ccgcgcgact atctggcaac cttcctgaaa atcggcgtta tcgtcggcct ggcgctgggt
     4021 atcgtggtgc tgaacccgga actgaaaatg cctgcgatga cccagtacat tgacggtact
     4081 ggcccgctgt ggaaaggtgc tctgttcccg ttcttgttca ttaccatcgc ctgtggtgcg
     4141 gtatctggct tccacgcact gatctcttcc ggtacgacgc cgaaactgct ggctaatgaa
     4201 accgacgcgc gtttcattgg ctacggcgca atgctgatgg agtccttcgt ggcgattatg
     4261 gcgctggttg ctgcgtccat catcgaaccg ggtctttact tcgcgatgaa caccccgcct
     4321 gctggccttg gcatcaccat gcctaacctg catgaaatgg gtggcgagaa cgcgccgatc
     4381 atcatggcgc agctgaaaga cgttaccgca cacgcggcag cgaccgtcag ctcttggggc
     4441 ttcgtgattt cgccagagca gatcctgcaa accgcgaaag acattggtga gccttctgtc
     4501 ctgaaccgtg caggtggtgc gccaacgctg gcggtaggta tcgctcacgt gttccacaaa
     4561 gtgctgccga tggctgacat gggcttctgg tatcacttcg gtattctgtt cgaagccctg
     4621 ttcatcctga ccgcgctgga tgcgggtacc cgttctggcc gttttatgct gcaagacctg
     4681 ctgggtaact tcatcccgtt cctgaaaaaa accgactctc tggttgccgg tatcatcggt
     4741 actgcgggct gtgtgggtct gtggggctac ctgctgtatc agggcgtggt cgatccgctg
     4801 ggcggcgtta agagcctgtg gccgctgttc ggtatctcca accagatgct ggcagccgta
     4861 gcgctggtac tgggcaccgt tgtgctgatt aagatgaagc gcacccaata catctgggta
     4921 actgttgttc cggctgtatg gctgcttatc tgcaccacct gggcgctggg tctgaaactg
     4981 ttcagcacca acccgcagat ggaaggcttc ttctacatgg caagccagta caaagagaag
     5041 attgctaacg gtactgacct gacggcgcag cagattgcca acatgaacca catcgttgtg
     5101 aacaactaca ccaacgcagg cctgagtatt ctgttcctga ttgtggtgta cagcatcatc
     5161 ttctacggtt tcaaaacctg gcttgcggtg cgtaacagcg acaaacgtac tgacaaagaa
     5221 actccgtacg ttccaatccc ggaaggcggc gtgaagatct cttcgcacca ctaaccgtgt
     5281 ttagccccgc ttcggcgggg ctttgttctt atcagagtga actatgtttg gtaacttagg
     5341 acaggcaaaa aaatatctcg gccaggcggc gaagatgttg attggcattc cagactacga
     5401 caactacgtc gagcatatga agaccaacca tcccgacaag ccgtacatga gctatgaaga
     5461 attcttccgc gaacgccaga acgcgcgcta cggcggcgat ggtaaaggcg gtatgcgctg
     5521 ttgttaatgg agagagcatg aacccgattg cagttaccct actcaccggt tttttaggcg
     5581 cgggcaaaac caccctgctg cgccatattc ttaacgaaca acacggctac gagattgccg
     5641 tgattgaaaa cgaattcggc gaagtctccg ttgatgatca actgattggc gaccgcgcca
     5701 cgcagatcaa aacgctgacc aacggctgca tctgctgttc gcgctccaac gagctggaag
     5761 acgcactact cgacctgctg gataatctcg acaagggcaa tattcagttc gaccgtctgg
     5821 tcattgaatg caccggcatg gccgatcccg gcccgattat tcagaccttc ttctcccatg
     5881 agattttatg ccagcgctac ctgctggacg gggtgattgc gctggtggat gcggtacatg
     5941 ccgatgagca gatgaaccag ttcaccatcg cccagtccca ggttggctac gccgaccgta
     6001 ttctgctgac gaaaaccgac gtcgcaggcg aagcagagaa actgcgtgaa cgcctggcgc
     6061 gcatcaacgc ccgcgcaccg gtctacaccg ttacccacgg cgatatcgac ctgggccttc
     6121 tgttcaacac caacggtttt atgctggaag aaaacgtcgt cagcaccaaa ccgcgtttcc
     6181 actttatcgc ggacaaacaa aacgatattt cgtcgattgt ggtggaactg gattacccgg
     6241 tagatatcag cgaagtttcc cgggtgatgg aaaacctgct gctggaatcg gcggataaac
     6301 tgctgcgtta caaagggatg ctgtggattg acggcgaacc taaccgcctg ctgttccagg
     6361 gcgtccagcg cctctacagc gccgactggg accgcccgtg gggcgatgaa aaaccgcata
     6421 gcacgatggt gtttatcggg attcagttgc cggaggatga gattcgggcg gcgtttgcgg
     6481 gactcaaaaa gtaaccagcc ctccaacatc acaatacctg agactttatc accctcatat
     6541 tcctccagaa tatgagggca ttcgggctta attatctctg aacgtgcaac acttcctgcg
     6601 actccttatg cgcctccaca tcctgcttcg ttaaccagag cgacttacga ccaaaatttt
     6661 cgtacatttt cagctgatct tcatagtgct gatcaactgt tccatcggga gcaataaacc
     6721 cactctgacc gggtgcgacc acatcccagg caagcaccgg acgatcgctt gtcgttggtg
     6781 agaaaacaat catatcgttt tctgttccac ggttttgata ctccgcctga tgacgcgttt
     6841 cttccgctgc ggcctgcggt acaccaaaga aattatttgc acggaacgtt aaggccattg
     6901 caggtgtttt ccagttactc acattattgc catagcgttt ggaaagagtc tcccaggtat
     6961 cttccagcgc agccaacaca acctcctgct gtggtttccc agcaaacaga tcaaccgcct
     7021 gtgggattgg tgatttgtct ccctgcaccg cctcatacaa aatttttgct ccaacactta
     7081 tattcagcga accagttggg ccgtcctggg ttgtttcgta gccactggcg ctgtaccact
     7141 tatcaaatgg cataggtacg gcagccacta cggtacgctt caacatactg gtcagccaaa
     7201 cgttcaggat ggcagagcct ggctgctgcc aggttttacc atcatcatta agcaaattga
     7261 tgccatccca acgtgttaat gtatccacca actgacggcg cggatcgctc tgtgtcaaac
     7321 cagatgtcgc tgcttgcaga gtaggtaaaa aaagcctcag gttaagatcc tgacgactgg
     7381 tttggcgaat aacatcccat gcctgatcag cagttaagcg tggcttttgc tcaagcagtc
     7441 ggtcgatctc cgtaacgcga
//
LOCUS       DAJTVJ010000178         7444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000178 DAJTVJ010000000
VERSION     DAJTVJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.178"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1252
                     /locus_tag="OO448_003902"
     CDS             <1..1252
                     /locus_tag="OO448_003902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6338996.1"
                     /translation="ENMLLEYVERKISLALSKYPKVRDVIKFFYLYIASLFGIILNKN
                     KTVIQSKIYEISIDDSEESFFGYYDHSPMSSNGRYVLFHSSAFSTKRHPKKVKYISIC
                     VKDLLNNKVYKLYDTRAFNWQQGSRLMWIDDDNIIFNDYENNGYISVVYSLSLMKVIK
                     KINYPIYDVNNYKAVTLDFSWLAKYDSDYGYYNKKSFSTDISIINLNTGGIELFLSLD
                     EMLKRTNFKCNIDVEHVVNHFMFAPDGRSVMFIHRYYTPKGKRERLIHWNLINDNVRV
                     LINESIISHCCWNGNDEIIGFFGAEIDSLNYYRLSIESCNTEKLFFDARKYSDGHPTI
                     VHNRYIISDTYPDKNRIKKLFVYDLVKNDYRELGLFYESMSFFSYSRCDLHPRISVDN
                     RFLFVDSVHSGKRKLYFMRSGICE"
     gene            1245..2231
                     /locus_tag="OO448_003903"
     CDS             1245..2231
                     /locus_tag="OO448_003903"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6338997.1"
                     /translation="MSDVLVSLIIVCFNAEKYIEKSLLAFINQDVGLDKFELIIVDGD
                     SSDNTISIVQDVFSKHSNIKHKIINNKKRTLATGWNIGVLEANGKFVCRVDAHSDIPN
                     NYISKLLDDYFNIMQFDDSVVGVGGVLTNSYKTKFGSIVADFYASKFGVGNSPFRCVD
                     KNNRLKKTDTAVFALYNKDVFFDVGLFNEVLDRNQDIDFHKRVLSNNLSLYTDNSLFV
                     EYYVRDNFKDFIKKGFLDGFWVVMSGAYYFRHIVPLFFVLYLIVSFSLFFATGDYIYL
                     SFLFFYFLISILFSIRDGRSFIGRVFLPFIFLSYHISYGCGSLLSFLKRYFK"
     gene            2228..3382
                     /locus_tag="OO448_003904"
     CDS             2228..3382
                     /locus_tag="OO448_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HCR6338998.1"
                     /translation="MKNFIPFALPEIGEEEIAEVIDSLRSGWITTGPKAKQFEQEFSN
                     YLGANVQSLAVNSATSGLHLALEAVGVKPGDQVIVPSYTFTATAEIVRYLGADPVIVD
                     VDRKTFNISVDAIEKAITNETKAIIPVHFAGLACDMDSILSIAKKYDLKVVEDAAHAF
                     PTTYKGSKIGTLDSDATVFSFYANKTMTTGEGGMVVSKNKDIIERCKVMRLHGISRDA
                     FDRYQSKTPSWFYEVVAPGFKYNMPDICAAIGIHQLRKIDDFQKKRQRMAKIYDDALK
                     ELPLELPEWPTNASDIHAWHLYPIRLKTDSAINRDDFIKKLSDLGIGCSVHFIPLHKQ
                     PVWRDTYNLNASDFPVSEECYLNEISIPLYTKMTDQDQLFVIKSIRQLFM"
     gene            3399..3989
                     /locus_tag="OO448_003905"
     CDS             3399..3989
                     /locus_tag="OO448_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR6338999.1"
                     /translation="MKRIFDVIVAGLGLLFLFPVFIIVSMLIVADSKGGVFFRQYRVG
                     RFGKDFRIHKFRTMFIDSEKKGRITVGQDARVTRVGWYLRKYKIDELPQLIDVLSGTM
                     SLVGPRPEVREFIDEYPDDIREKVLSVRPGITDLASIEMVDENEILSSYDDPRRAYID
                     IILPIKQRYYLDYVANNSVKYDCVIIWKTIIKILSR"
     gene            4145..5923
                     /locus_tag="OO448_003906"
     CDS             4145..5923
                     /locus_tag="OO448_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR6339000.1"
                     /translation="MWFAAAILTIFTVFIFIRIGLYRAVLRYVSAKIMLLIPVGILAS
                     TLSLVVISYSLSIMLPRTVVGIYFLVLLLLTSGSRLLFRMILNYGVKGSAPVLIYGAG
                     ESGRQLLPALMQAKEYFPVAFVDDNPRLHKAVIHGVTVYPSDKLSYLVDRYGIKKILL
                     AMPSVSKSQRQKVITRLEHLPCEVLSIPGMVDLVEGRAQISNLKKVSIDDLLGRDPVA
                     PDAKLMAENITGKAVMVTGAGGSIGSELCRQIVRYKPAKLVLFELSEYALYAIEKELS
                     ALCDKEVLNVPVIPLLGSVQRQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVR
                     NNVFGTLYCAESAIESGVETFVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTR
                     FCMVRFGNVLGSSGSVVPLFEKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMG
                     HGGDVFVLDMGDPVKIYDLAKRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEEL
                     LIGDSVQGTSHPRIMTANEVMLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFN
                     PTDDICDLVWQQKKSLLSQASNVIRL"
     gene            6042..6549
                     /gene="aqpZ"
                     /locus_tag="OO448_003907"
                     /pseudo
     CDS             6042..6549
                     /gene="aqpZ"
                     /locus_tag="OO448_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
     gene            6707..7444
                     /locus_tag="OO448_003908"
                     /pseudo
     CDS             6707..7444
                     /locus_tag="OO448_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS629 family transposase"
     misc_feature    6988..7104
                     /locus_tag="OO448_003908"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         2062 a         1244 c         1696 g         2442 t
ORIGIN      
        1 tgagaatatg ttgttagaat atgttgaaag aaaaatttcc ttagccttga gtaagtatcc
       61 taaggtaagg gatgttatta agttctttta tttatatatc gcatcattat tcggaattat
      121 tttgaataaa aataagacgg ttattcaatc aaaaatatac gagatttcaa ttgatgattc
      181 tgaagaatca ttttttggct attatgacca tagtccaatg agctctaatg ggcggtacgt
      241 attgttccac tctagtgcgt ttagcactaa acgacatcca aagaaagtta agtatatatc
      301 tatttgcgta aaagaccttc ttaataacaa agtttataag ctatatgata cgcgagcatt
      361 taattggcag cagggaagcc gattaatgtg gattgatgat gacaatataa tttttaatga
      421 ctatgaaaat aatggataca ttagtgttgt ctattctttg tctttgatga aggttataaa
      481 aaaaataaac tatccgattt atgatgtgaa taattacaag gctgtgacgt tagatttctc
      541 atggctggct aaatatgata gcgattatgg ttattataat aaaaaatcat tttctacaga
      601 tatttcaatc attaatttga atacgggggg aatagaatta tttttatcct tagacgaaat
      661 gctaaagaga actaatttta aatgtaatat tgatgttgaa catgtggtca atcattttat
      721 gtttgctccc gatggacgtt ccgttatgtt catacatcga tactatacac ctaaaggaaa
      781 gcgtgaaagg ttaatacatt ggaatttaat aaatgataat gttcgagtcc taataaatga
      841 atcgattatt agtcattgtt gttggaatgg gaatgatgaa attataggtt tttttggtgc
      901 agaaatagat tcgctaaatt attatagatt gtcaattgaa tcctgtaata cagagaaatt
      961 gttttttgat gcaagaaaat attctgatgg acatcctact atagttcata atagatatat
     1021 tatatctgat acttacccag ataaaaatag aattaaaaag ttgtttgttt atgaccttgt
     1081 caaaaatgat tatcgcgagc ttggattatt ttatgagtca atgagttttt tttcttattc
     1141 tcgatgtgac ttacatccaa ggatctcggt tgataataga tttttgtttg ttgattcagt
     1201 tcactcaggg aaaagaaaac tatattttat gaggagtggt atttgtgagt gatgttctag
     1261 tatctttaat tatagtttgc tttaatgcag agaagtatat tgaaaaatct cttttggcat
     1321 ttattaatca agatgttgga ttagataaat ttgaattgat tattgtagat ggggattcat
     1381 ctgataatac aatatctatt gttcaggatg ttttttctaa acatagcaac attaagcata
     1441 aaattatcaa taataaaaaa agaactcttg ctacgggttg gaatattggg gtgctagaag
     1501 ctaatggtaa gtttgtgtgt agagttgatg cacatagtga tattccaaat aactatatat
     1561 ctaaattatt agatgattat tttaatatta tgcagtttga tgatagcgtt gttggtgttg
     1621 gaggtgtatt aactaattct tataaaacta agtttggttc aattgtagcg gatttttatg
     1681 catcgaaatt tggtgttggt aattctccat ttaggtgcgt agacaaaaat aatcgactaa
     1741 aaaaaacaga tacagctgtc tttgctttat ataataaaga tgtgtttttt gatgttggac
     1801 tttttaatga agtattagat agaaatcaag atattgattt tcataagaga gttttaagca
     1861 ataatttgtc attatataca gataatagtt tatttgttga gtattatgtt agagataatt
     1921 ttaaagattt cataaagaaa ggttttcttg atggtttttg ggttgttatg tctggagcat
     1981 attattttag acatatagtg ccactttttt ttgttttgta tttaattgta tctttttctc
     2041 ttttctttgc tactggtgat tatatatatt tatctttttt atttttttat tttcttattt
     2101 ctattttgtt ttcaattcga gatgggcgaa gttttatagg tagagtattt cttcctttta
     2161 tatttttgtc ttatcatatt tcttatggat gtggatcgtt attatctttt ttgaaaaggt
     2221 attttaaatg aaaaatttta ttccttttgc gttacctgaa attggcgaag aagaaattgc
     2281 agaggtaatt gactctttac gttcaggttg gattacgaca ggtcctaagg ctaagcaatt
     2341 tgaacaagaa ttttctaatt acctaggagc gaacgttcaa tcattagctg ttaactctgc
     2401 tacgtcgggc ttacatttgg ctcttgaagc tgttggcgta aagccgggag accaagttat
     2461 tgtcccatca tatacattca ctgctactgc cgaaattgtc aggtaccttg gtgctgatcc
     2521 tgtaattgtt gatgtagatc gtaaaacatt taatatatca gttgatgcca ttgagaaggc
     2581 tattactaat gaaacaaagg cgattattcc agtacacttc gctggattag cttgtgacat
     2641 ggattcaatc ttatcaattg ctaaaaaata tgacctaaag gttgtcgagg atgccgctca
     2701 tgcatttcct acaacatata aaggaagtaa gataggaacg cttgattcag atgctacggt
     2761 ttttagcttc tacgccaata aaactatgac aaccggtgaa ggcggaatgg ttgtttcaaa
     2821 aaataaagat ataattgagc gttgtaaggt aatgcgttta catggaatca gtcgtgacgc
     2881 ttttgaccgg taccagtcta aaactccttc ttggttttat gaggttgtag ctccagggtt
     2941 taaatacaat atgcctgata tctgtgcggc aatcggtatt catcaactta gaaagatcga
     3001 tgattttcag aaaaaacgtc aacgaatggc aaaaatttac gatgatgcgt taaaagaatt
     3061 gccacttgaa ttgcctgaat ggcctactaa tgctagtgat attcatgctt ggcatctata
     3121 tcctatccgc ttaaaaactg attcggctat taatcgcgat gattttatta agaagttatc
     3181 agatcttgga attggttgtt ctgtccattt tataccgttg cataagcaac cggtttggcg
     3241 tgatacatat aatttgaacg ccagtgactt tccagtttct gaggagtgtt atttaaatga
     3301 aatatctatt cctctttata ctaaaatgac ggatcaagat cagttgttcg ttatcaaatc
     3361 gattagacaa ttatttatgt aatggtattt tatattaaat gaaacgtatt tttgatgtta
     3421 tcgtggcagg cttaggcctg ctttttctat ttcctgtttt tatcattgtg tcaatgttaa
     3481 ttgttgctga ttctaaaggg ggggtttttt ttaggcagta tagagttggg agatttggga
     3541 aagattttag gatacataaa tttagaacga tgtttatcga ttcagaaaaa aaaggacgga
     3601 taacagttgg tcaagatgct cgggtaacca gagttggatg gtatttacgg aagtacaaaa
     3661 tcgatgagct tcctcaattg atagatgttc tttctggaac aatgagtttg gttggcccaa
     3721 gaccggaagt gagggagttt attgatgagt atcctgatga tataagggaa aaagttttat
     3781 cggttaggcc agggataact gacttagcat ctatagaaat ggtagatgaa aatgagattt
     3841 tgtctagtta tgatgaccca cgtagggctt atatagatat aattcttcca atcaagcaaa
     3901 gatattattt agattatgtt gctaacaatt cagtaaagta tgattgtgtg ataatttgga
     3961 aaactattat taagattttg tcgcgataat aaggtagtgt aggatgattg atagaatatt
     4021 ggagctgcca agaattgtta agagaggtat catcatctgc attgatgtag ttatggtgat
     4081 attctcattt tggttgtctt attggttgag gcttgatgag caaacggctt ttcttagtgc
     4141 accgatgtgg tttgctgcag ctattcttac catatttacc gtgtttatat ttatcaggat
     4201 tgggctttat cgggcagtct tacggtatgt tagtgcaaag ataatgttgc taataccagt
     4261 tggtattctg gcctcaacgt tatctcttgt cgttatatca tattcgctat ccataatgtt
     4321 gccgcgcact gttgtcggaa tttatttttt ggttttactt ttactgacat caggctctag
     4381 attgcttttt agaatgatac ttaactatgg agttaagggt agtgcgcctg ttttgattta
     4441 tggcgctggt gaatctggcc gacaattatt gccagcatta atgcaggcaa aagaatattt
     4501 tcctgtggca tttgtggatg ataatcctcg cttgcataag gctgtcattc atggtgtaac
     4561 agtttatccc tcggataaac tgagttacct tgtagatcgc tatggtataa agaaaattct
     4621 tttggcgatg ccgagcgtca gtaagtcaca aaggcagaaa gtgattactc gtttagagca
     4681 tctaccgtgt gaagttctct ctattccggg tatggtcgat ttagtcgaag gtcgagcaca
     4741 aatcagtaat ctaaaaaaag tatcgattga tgacttacta ggtcgtgatc cggttgctcc
     4801 tgatgccaaa ttgatggccg aaaacattac tggcaaagcc gttatggtca ctggggcggg
     4861 aggctcgatc ggctctgagc tttgtcgtca aattgttcga tataagccgg ccaaattggt
     4921 tctatttgaa ctgtctgaat atgccctcta cgctattgag aaagagctct cggcgctgtg
     4981 tgacaaagaa gttttgaatg ttccagtgat ccctctgttg ggctcggtgc agcgtcagaa
     5041 tcgcttacag atggtgatga agtcctttgg tattcaaacg gtttatcatg cggccgctta
     5101 taaacatgtg cctctggttg agcataatgt ggtggaaggg gtacgtaata acgtgtttgg
     5161 taccttgtac tgcgctgagt cagcgatcga aagtggcgtt gaaacttttg tgttgatttc
     5221 caccgataaa gcggtgcgcc cgaccaacac tatggggaca actaagcgtc tggccgaatt
     5281 ggtattgcag gctttgtctg cacggcaaag ccaaactcgc ttttgtatgg tgcgatttgg
     5341 taatgtactc ggttcttcgg gctctgtcgt gccgttgttt gaaaaacaga ttgcccaagg
     5401 tgggccagtt accttgactc atcgtgacat tattcgctat ttcatgacaa ttccggaagc
     5461 atcacagttg gtgattcaag cgggggcgat ggggcatggc ggcgatgtct ttgtcttaga
     5521 catgggcgat ccggtcaaga tttatgactt agccaaacgc atgatccggt taagtggctt
     5581 gagtgtacgg gatgataaaa atccagatgg cgatattgcc attgaagtta cgggattacg
     5641 tccaggtgag aaactgtatg aagaattact gattggtgat tcagttcaag gtacctctca
     5701 tccacgaatt atgacggcca acgaagtgat gctaccgtgg caggatctat cgctcttact
     5761 taaagagctg gatcaagctt gtcatgactt tgatcatgag cgaattcgca gtttgttgtt
     5821 acaagcacca gcggcattca atccaactga tgatatttgc gatctagttt ggcagcagaa
     5881 aaaatcgctg ttatcacaag cgagcaatgt cattcgcctg tgattgctta ggtttaacct
     5941 tccacaccaa ttcttcacct ctcttacaaa tccccgctag gcggtacatc gtgaccgcct
     6001 ttaccctgat gcctgctctt taacaaacag gacatcagtg tatgtttaaa ccttttagcg
     6061 ccgaattttt cggcactttc tggctggttc tgggtggctg tggtagcgcc ttgatctctg
     6121 ctgctttccc acagttaggt ataggctttt tgggcgtggc gttggcgttt ggtctgacag
     6181 tagtcaccat ggcttatgcg gtcgggcaca tctctggtgc gcattttaac cccgcggtga
     6241 ccttgggtct gtgggccggt ggacgcttcc cagcagcgcg cgtgttacct tacattatcg
     6301 ctcaggttat cggcggtatt gccgctgcgg cagtgctgta tggtatcgcc agcggtaagg
     6361 ctgggtttga tgcgacaacc agcggttttg cggctaatgg ttatggcctc cattcacctg
     6421 gcggctatgc gttaagcgcc tgtatgctga gcgagtttgt cctcagtgcg ttttttgtcc
     6481 ggagcgacag aaaaacgcgc tcctgcgggc tttgcgccac tggcgattgg tctggtaatc
     6541 accccgtaaa ttaaccagcg tcaaaagtag aattttctcg taccataaac gcaggagatt
     6601 ctttatgaaa acatcaaaat ttaccgacag ccaaatcatg gcgatcctca aatgaaccgc
     6661 cccgggaatc ctggagacta aacttcctga gaaagaggta aacaggatga ctaaaaatac
     6721 tcgtttttcc cccgaagtcc gtcaacgggc agtccgtatg gttctggaaa gtcagggcga
     6781 atatgactca caatgggcga caatttgttc cattgctcca aagattggct gtacgccgga
     6841 gactctgcgt gtccgggttc gccagcatga gcgggatacc gggggcggtg atggagggct
     6901 caccaccgct gaacgtcagc gtctgaaaga gctggagcgt gaaaatcgtg aactgcgccg
     6961 cagtaacgat atccttcgcc aggcttccgc ttattttgcg aaggcggagt ttgaccgcct
     7021 ctggaaaaaa tgatgccact gctggataag ctgcgtgagc agtacggggt cggaccgcta
     7081 tgcagcgaac tgcatattgc cccgtcaacg tattaccact gtcagcaaca gcgacatcat
     7141 ccggataaac gcagtgcccg tgcgcagcgc gatgactggc tgaagaaaga gatacagcgc
     7201 gtatacgatg aaaatcacaa ggtatacggt gtgcgtaaag tctggcgtca gttgttacgg
     7261 gaaggtatca gagtggccag atgcactgtg gcacgtctca tggcggttat gggacttgcc
     7321 ggtgttctcc ggggtaaaaa ggtccgtacg accatcagcc ggaaagccgt tgccgcaggc
     7381 gaccgcgtaa accgtcagtt cgtggcagaa cgacctgacc agctgtgggt ggctgatttt
     7441 actt
//
LOCUS       DAJTVJ010000179         7437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000179 DAJTVJ010000000
VERSION     DAJTVJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.179"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(97..1605)
                     /gene="putP"
                     /locus_tag="OO448_003909"
     CDS             complement(97..1605)
                     /gene="putP"
                     /locus_tag="OO448_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR6339001.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2027..5989
                     /gene="putA"
                     /locus_tag="OO448_003910"
     CDS             2027..5989
                     /gene="putA"
                     /locus_tag="OO448_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6339002.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6029..6667)
                     /gene="rutR"
                     /locus_tag="OO448_003911"
     CDS             complement(6029..6667)
                     /gene="rutR"
                     /locus_tag="OO448_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6339003.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6955..>7437
                     /locus_tag="OO448_003912"
     CDS             6955..>7437
                     /locus_tag="OO448_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6339004.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1775 a         2064 c         1947 g         1651 t
ORIGIN      
        1 gcgcgctccc tgcaccattc aatttatatg gcgaggcaca ttaagggagc gcaacccgct
       61 gctgaagcca aacaaaaccg ccgcaggcta agtcccttag ttttcctgca accgtgacgg
      121 cggagccgaa tgatagtgcg catcggcctc ggcaaagcgt ttttgcatcg ccgctgacgg
      181 cgctttaccc agcaaactaa acactacaat cccgatactg ccgaagatga agcccggaat
      241 aatttcgtac agtcccagcc agccgaactg cttccagacg ataactgtca gcgcaccgat
      301 gatcatcccc gccagcgcac cgttgcgcgt catgcgtgac cacatcaccg agaacagcac
      361 caccggacca aacgccgcgc caaagcctgc ccacgcgtag ctcactaagc ccagcacgcg
      421 gttttccggg tttgccgcca gcgcaatcgc caccagcgcc accaccagca ccatcacacg
      481 tcctacccac accagctctt tctggctggc ctgtttacgc agaaacgctt tgtataaatc
      541 ttcggtaatc gcactggagc acaccagcag ctggcaactt aaggttgaca ttaccgccgc
      601 cagaatcgcc gacagcagaa tcccggcaat ccacgggtta aacagaattt gcgccagttc
      661 gataaacacg cgttcagcgt tctggtttac cgcacctgct accgccggat gctcgttaaa
      721 gtaagcgata ccaaagaagc caacagccac cgcccctgcc aggcaaagga tcatccaggt
      781 catactgata cgacgcgcat ggacaatgct gtggtgagaa tctgccgcca taaaacgcgc
      841 caggatgtgc ggctgcccga agtaacccag cccccaaccc atcaatgaga taatggcaac
      901 aaagttcagc cctttgagca tatccacgtt ttcgatgctc ttttgtttga tcacttccag
      961 cgagtcacca aagccaccga cactgataat gacgataacc ggcgtcagga tcagggcaaa
     1021 aatcatcagg ctggcctgta cagtgtcagt ccagctcacc gcgaggaaac cgccaataaa
     1081 ggtgtaaagg atcgtcgacg cagctcccgc caacagagcc gtttcgtaac tcatgccaaa
     1141 ggtactttca aacagacgcg cgcctgccac aatgcccgaa gcgcaataaa tggtgaagaa
     1201 cagcaaaata accagcgcgg agataatgcg caaaatgcgg cttttatctt caaaacgccc
     1261 ggtgaaataa tccggcaatg ttaaggcgtt attattgtat tcggtatgca cacgcaaccg
     1321 cccggccacc agcttccagt tgatccacgc gcctaatgtc aggccaatgg cgatccagct
     1381 ttcggaaatc ccggaaagaa aaaccgcgcc aggcaacccc attaacagcc agccgctcat
     1441 atccgacgca cccgccgata atgccgtcac gaatggccca agactacgac cgcccagaat
     1501 atagtcgtca aagtttttcg ttgatcgcca ggcgataaac ccaatcaata tcatgccaaa
     1561 gatatagaca caaaatgtca ccaacatcgg tgtgctaata gccatctaaa gtctccaaaa
     1621 aattattatc ggcaatgtcg aaacttgccg ttatatctgc caccggaacg gggtaacaga
     1681 gtttatgttt taccagggcg accgtatcct gccggaagcg ctggttattc acaatcgatt
     1741 taacacacca tttacattaa attttagtgc tcagcgacac tatttttcat caggttgcac
     1801 tctctcacat tttttgcggt tgcacctttc aaaaaggtta actgccgcag agaaaaagtc
     1861 tgagttattt tttaatcact gtcatatcga tttcttttat taacatttca ttcattttta
     1921 agcttgctac gcaagtcaca tttaacatgg ttgcacaaag ttgcaacatc atggatattt
     1981 cacgataacg ttaagttgca cctttcagaa caacaggagt aatggcatgg gaaccaccac
     2041 catgggggtt aagctggacg acgcgacgcg tgagcgtatt aagtctgccg cgacacgtat
     2101 cgatcgcaca ccacactggt taattaagca ggcgattttt tcttatctcg aacaactgga
     2161 aaacagcgat actctgccgg agctacctgc gctgctttct ggcgcggcca atgagagcga
     2221 tgaagcaccg actccggcag aggaaccaca ccagccattc ctcgactttg ccgagcaaat
     2281 attgccccag tcggtttccc gcgccgcgat caccgcggcc tatcgccgcc cggaaaccga
     2341 agcggtttct atgctgctgg aacaagcccg cctgccgcag ccagttgctg aacaggcgca
     2401 caaactggcg tatcaactgg ccgataaact gcgtaatcaa aaaaatgcca gtggtcgtgc
     2461 aggaatggtc caggggttat tgcaggagtt ttcgctgtca tcgcaggaag gcgtggcgct
     2521 gatgtgtctg gcagaagcgt tgttgcgtat tcccgacaaa gccacccgcg acgcgttaat
     2581 tcgcgacaaa atcagcaacg gtaactggca gtcacacatt ggtcgtagcc cgtcactgtt
     2641 tgttaatgcc gctacctggg ggctgctgtt tactggcaaa ctggtttcca cccataacga
     2701 agccagcctc tcccgctcgc tgaaccgcat tatcggtaaa agcggtgaac cgctgatccg
     2761 caaaggtgtg gatatggcga tgcgcctgat gggcgagcag ttcgtcacgg gtgaaaccat
     2821 cgcggaagcg ttagccaatg cccgcaagct ggaagagaaa ggtttccgtt actcttacga
     2881 tatgctgggc gaagctgcac tgactgccgc agacgcacag gcgtatatgg tttcctatca
     2941 gcaggcgatt cacgccattg gtaaagcgtc taacggtcgt ggcatctatg aaggtccggg
     3001 catttcaatc aaactgtcgg cgctgcatcc gcgctacagc cgcgcccagt atgaccgggt
     3061 aatggaagag ctttacccgc gtctgaaatc actcaccctg ctggcgcgtc agtatgatat
     3121 tggtattaac atcgatgccg aagaggccga tcgtctggag atctccctcg atctgctgga
     3181 aaatctctgt ttcgagccgg aactggcagg ctggaacggc atcggttttg ttattcaggc
     3241 ttatcaaaaa cgctgcccgt tggtgatcga ttacctggtt gatctcgcca cccgcagccg
     3301 tcgccgtctg atgattcgcc tggtgaaagg cgcgtactgg gatagtgaaa tcaagcgtgc
     3361 gcagatggac ggccttgaag gctatccggt ttatacccgc aaggtgtata ccgacgtttc
     3421 ttatctcgcc tgtgcgaaaa agctgctggc ggtgccgaat ctaatctacc cgcagttcgc
     3481 gacgcacaac gcccatacgc tggcagcgat ttatcaactg gcaggtcaga actactatcc
     3541 gggtcagtac gagttccagt gcctgcatgg tatgggcgag ccactgtacg agcaggtcac
     3601 cgggaaagtt gccgacggta aacttaaccg accgtgtcgt atttatgccc cggttggcac
     3661 acatgaaacg ctgctggcgt atctggtgcg tcgcctgctg gaaaacggtg ctaacacctc
     3721 gtttgttaac cgtattgccg acacctcttt gccactggat gaactggtcg ccgatccggt
     3781 cactgctgta gaaaaactgg cgcaacagga agggcaaact ggattaccgc atccgaaaat
     3841 tcccctgccg cgcgatcttt acggtcacgg gcgcgacaac tcggcagggc tggatctcgc
     3901 taacgaacac cgtctggcct cgctctcctc tgccctgctc aatagtgcac tgcaaaaatg
     3961 gcaggccttg ccaatgctgg aacaaccggt agcggcaggt gagatgtcgc ccgttattaa
     4021 ccctgcggaa ccgaaagata ttgtgggctt tgtgcgtgaa gccacgccac gtgaagtaga
     4081 acaggcgctg gaaagtgcgg ttaataacgc gccaatctgg tttgccacgc ctccggctga
     4141 acgcgcggcg attttgcatc gcgctgccgt gctaatggaa agccagatgc agcaactgat
     4201 tggtattctg gtgcgtgaag ccggaaaaac cttcagtaac gccattgccg aagtgcgcga
     4261 agcggtcgat tttctccact actacgccgg acaggtgcgg gatgatttcg ctaacgaaac
     4321 ccaccgtcca ttagggcctg tggtgtgtat cagtccgtgg aacttcccgc tggctatttt
     4381 caccgggcag atcgctgccg cactggcggc aggtaacagc gtgctggcaa aaccggcaga
     4441 acaaacgccg ctgattgccg cgcaagggat cgccattttg ctggaagcgg gtgtaccccc
     4501 tggcgtggtg caattgctgc caggtcaggg ggaaaccgtg ggcgcgcaac tgacgggtga
     4561 tgatcgcgtg cgcggggtga tgtttaccgg ttcaaccgaa gtcgctacgt tactgcagcg
     4621 caatatcgcc agccgcctgg acgctcaggg tcgccctatt ccgctcatcg ctgaaaccgg
     4681 cggcatgaac gcgatgattg tcgattcttc agcactgacc gaacaagtcg tcgtggatgt
     4741 gctggcctcg gcgttcgaca gtgcgggtca gcgttgttcg gcactgcgcg tgctgtgcct
     4801 gcaagatgag attgccgacc acacgttgaa aatgctgcgc ggtgcaatgg ccgaatgccg
     4861 gatgggtaat ccgggtcgcc tgaccaccga tatcggtccg gtgattgata gcgaagcgaa
     4921 agccaatatt gagcgccata ttcagaccat gcgtagcaaa ggccgtccgg tgttccaggc
     4981 ggtgcgggaa aacagcgaag atgcccgtga atggcaaagc ggcacctttg tcgccccgac
     5041 gctgatcgaa ctggatgact ttgccgaatt gcacaaagag gtctttggtc cggtgctgca
     5101 tgtggtgcgt tacaaccgta accagctacc agagctgatc gagcagatta acgcttccgg
     5161 ctatggtctg acgcttggtg tccatacgcg tattgatgaa accatcgccc aggtcactgg
     5221 ctcggcccat gttggtaacc tgtatgttaa ccgtaatatg gtgggcgcag tggttggtgt
     5281 gcagccgttc ggcggcgaag ggttgtccgg taccgggccg aaagcaggcg gtccgctcta
     5341 tctctaccgt ctgctggcga atcgcccgga aagtgcgctg gcagtgacgc tcgcgcgtca
     5401 ggatgcagag tatccggtcg atgcacagtt gaaagccgca ttgactcagc cgctaaatgc
     5461 actgcgggaa tgggcagcaa atcgtccaga attgcaggcg ttatgtacgc aatatggcga
     5521 gctggcgcag gcaggaacac aacgattgct gccggggccg acgggtgaac gcaacacctg
     5581 gacgctgctg ccgcgtgagc gcgtgttgtg tattgccgat gatgagcagg atgcgctgac
     5641 tcagctcgcc gccgtgctgg cggtgggcag ccaggtactg tggccggatg acgcgctgca
     5701 tcgtcagcta gtaaaggcat tgccatcggc agtcagcgaa cgtattcaac tggcgaaagc
     5761 ggaaaatata accgctcaac cctttgatgc ggtgatcttc cacggtgatt cggatcagct
     5821 tcgcgcattg tgtgaagccg ttgctgcgcg ggatggcgca attgtttcgg tgcagggttt
     5881 tgcccgtggc gaaagcaata tccttctgga acggctgtat atcgaacgtt cgctgagtgt
     5941 gaataccgct gccgctggcg gtaacgccag tttaatgacg atcggttaaa caggtagccg
     6001 gaggatgtta caacctcctc cggcatcttt aacgtggtcg aatcccctca ataataatcc
     6061 gctgcacgtt ttcaaccgtt tgattgaaaa atatctcatc gcgcaacgtc gcgcctgtca
     6121 ccgcctccac ctgaggggcg aaatcggcgt aatgttgagt ggaagcccaa atcatgaaaa
     6181 tcaaatgctg cggatcaatc ggcgcgagtt tgccgctttt gacccaaccg gcaatcagcg
     6241 cagatttctc atcaattaat gccttcaaat cgcccgtcag ttcatccatt aacagcggcg
     6301 cgcctgccag catctccata cagaacaggc gcgaagcctg cggataatcg cgggagactt
     6361 ccagcttcag acggatgtac tcttttatcg ccgccagcgg agcgaaatct tcacgaaacg
     6421 cttttaacgg tgccagccag atatcgagaa tctgccgcag cacggcaata tacagcgcct
     6481 cttttgacgg aaagtaatac agcagattgg tttttgaaac acccgccaac tctgcgatct
     6541 gctccagcct tgtgccgtga aaaccgaatt gtgaaaaagt gtccagtgct gcgctaagaa
     6601 tcgctttttt cttcgcgctt actgcgcgcg aacgtttacc cgttgttttc actgcgcctt
     6661 gcgtcatgcg ctctcccctc ttgtagattg gcaacaggat agcaaaaccc ccgcccggcc
     6721 tcgaccttct gcactctcat cgcgcacaga tgcatgtttt atgtgcaact gttttgaccg
     6781 tttagtccac tttttatcag atattttaga taacgtaata ttaacatatt aataacatta
     6841 actttttaaa aactggcatc cgctttgcaa acaagccaat acgcggctga aaaggatatg
     6901 caggatgcag cgccccgtct gactttcact ttacgagacg aagagaggtt gatgatgaaa
     6961 attggcgtat tcgtacctat tggcaacaac ggctggctca tttcgaccca cgcgccgcag
     7021 tacatgccga cctttgaact gaataaagcc attgtgcaga aagcggagca ttaccatttc
     7081 gatttcgccc tgtcgatgat caaactgcgt ggctttggcg gcaaaactga gttctgggat
     7141 cacaaccttg agtcgttcac cttgatggcg ggtctggcag ccgtgacctc gcgcattcag
     7201 atttacgcca ctgctgccac cttaacgtta cctccggcaa tcgtcgcccg tatggccgca
     7261 accatcgact ccatctctgg cgggcgtttt ggcgtcaacc tcgtgactgg ctggcaaaag
     7321 cccgtgtatg agcagatggg tatctggcct ggcgatgact atttctcccg tcgttacgac
     7381 tatctcaccg aatatgttca ggtgctgcgc gacctgtggg gcacggggaa aagcgat
//
LOCUS       DAJTVJ010000180         7418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000180 DAJTVJ010000000
VERSION     DAJTVJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.180"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..88)
                     /locus_tag="OO448_003913"
     CDS             complement(<1..88)
                     /locus_tag="OO448_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCR6339005.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPA"
     gene            complement(113..1213)
                     /locus_tag="OO448_003914"
     CDS             complement(113..1213)
                     /locus_tag="OO448_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6339006.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1601..2347)
                     /gene="cutC"
                     /locus_tag="OO448_003915"
     CDS             complement(1601..2347)
                     /gene="cutC"
                     /locus_tag="OO448_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6339007.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2361..2927)
                     /locus_tag="OO448_003916"
     CDS             complement(2361..2927)
                     /locus_tag="OO448_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6339008.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3143..4879
                     /gene="argS"
                     /locus_tag="OO448_003917"
     CDS             3143..4879
                     /gene="argS"
                     /locus_tag="OO448_003917"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6339009.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5056..5418
                     /locus_tag="OO448_003918"
                     /pseudo
     CDS             5056..5418
                     /locus_tag="OO448_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5422..6492)
                     /locus_tag="OO448_003919"
                     /pseudo
     CDS             complement(5422..6492)
                     /locus_tag="OO448_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6485..>7418)
                     /gene="flhB"
                     /locus_tag="OO448_003920"
     CDS             complement(6485..>7418)
                     /gene="flhB"
                     /locus_tag="OO448_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCR6339010.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1941 c         1775 g         1782 t
ORIGIN      
        1 atgcggggag cggtgctgcc gatgtcacca cctgcgctcc agcggcaata ccgctgtgtt
       61 taataaattc acgtcttgtt aatgtcataa cttcctccct gatcaacgag gatcactgtt
      121 tctcggtaat atctttggcg ttgtactgga aataccgcgt taaaatgtcc agttcgtttt
      181 cgctcatgct ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt
      241 aatggtcggc ggcaataggg gcatgacaac cagcgcaata ggtatcgtca agtttttcag
      301 cgtattgcca tagcggttta cggtccgcta atccgggatc ggtaagcgca ccctgtaaag
      361 acgcctgacg ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg
      421 ccttgatacc ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga
      481 gcgtgttttc actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac
      541 tggcatcaac gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag
      601 aagttttgaa aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca
      661 gctcatgcgc cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat
      721 gacaatcgat acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt
      781 cactttgcga ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt
      841 cagttgcttt taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact
      901 tatcgtcact gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta
      961 atttagcaaa taaataatcc atccctgact ttggaatatg gcaatctgca cattccgcac
     1021 gtatcccttt ctggttcgaa aagtggacag ttccctgata ttcctcaaaa gatttactca
     1081 tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca
     1141 gcaataaccc gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt
     1201 ttttccctcg catagattcc tctaaataaa ttccagaaca ccggaatcat tgtataatta
     1261 aatacacaac gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata
     1321 aagaatatga tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt
     1381 agaatatata tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa
     1441 tattattagc acaccgctat gttcactgtc acccgctcaa cagatcacgg ttatcgttcg
     1501 ttttttatac tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg
     1561 agcaagcatc atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt
     1621 caatgattcc tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt
     1681 ccgcgtgttc atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt
     1741 gccacgctcc cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt
     1801 tttctgcacg gactcctgct ccggccataa tgattggagc atcacgatgg gcaataagtt
     1861 ccataatttt tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg
     1921 caatgcccaa ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg
     1981 cgcgatgaaa agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca
     2041 tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc
     2101 ggaccgtgcg cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac
     2161 caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca
     2221 gtacccccaa cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt
     2281 ctgcgccgtt ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta
     2341 atgccatttt tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac
     2401 cacggatgaa acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat
     2461 cgctcatgct caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct
     2521 tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cagcagaacg
     2581 atttcaatat gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc
     2641 acaatagaaa actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc
     2701 ggtctgccat tgatcatatt ttctgacaaa agctcgccac actgttcaaa cccgcgacgc
     2761 cagcgttcag cagtggcgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga
     2821 gtgatattca accccagacg gagggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa
     2881 tccgatgcaa tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt
     2941 ctttcagtaa aagcgttaat ttaccctgtt gccctgcgcc aaccaaccgc tgatttcacg
     3001 ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca
     3061 gccatttgca gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc
     3121 ttcatcaatg taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca
     3181 ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg caggttcgtc attcagcaaa
     3241 agttcagttc ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat
     3301 gacaccgcga caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag
     3361 caaagttgag atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc
     3421 tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt gtttctacgc cagaaaaaca
     3481 gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa gagatgcatg tcggtcacct
     3541 gcgctctacc attattggtg acgcagcagt gcgtactctg gagttcctcg gtcacaaagt
     3601 gattcgcgca aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct
     3661 ggaaaagcag cagcaggaaa acgccggtga tgaaatggag ctggctgacc ttgaaggttt
     3721 ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc gtgcacgtaa
     3781 ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc gcaaactggt
     3841 cgacatcacc atgacgcaga accagatcac ctacgatcgt ctcaacgtga cgctgacccg
     3901 tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg ccaggaattg tggcggatct
     3961 caaagccaaa ggtctggcag tagaaagcga aggggcgacc gtcgtattcc ttgatgagtt
     4021 taaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg gcggctatct
     4081 ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc atgccgatcg
     4141 cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcat gggcgatcgt
     4201 ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt tcggcatgat
     4261 gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtacagtga aactggccga
     4321 tctgctggat gaagccctgg aacgtgcacg ccgtctggtg gcagaaaaga acccggatat
     4381 gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg tgaaatatgc
     4441 ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca tgctggcgtt
     4501 tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt ccgtgttccg
     4561 taaggcagaa attaacgaag agcaactggc tgcagctccg gtaatcatcc gtgaagatcg
     4621 tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg tggttgcccg
     4681 tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggctggtc tgttctctgg
     4741 cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct
     4801 gaaactggca caactgacag cgaagacgct aaagctgggt ctggatacgc tgggtattga
     4861 gacagtagag cgtatgtaat cgatttttcg tgagagtaaa gcctgatcag ggttacctga
     4921 tcaggctttt ttattgccat ctaaatgtat tctgaaaatg gacatgccat tgttttctga
     4981 ctgttggata agaggccaga agcgtaatat ccggccccag ggaaacgata acggttgaat
     5041 ttaaggaata ccgcagtgtt taaatttctt gtattaacat taggcattat ctcttgccag
     5101 gcttacgcag aagatacggt tatagaaaac gaccatgaca tttcagccat caaagattgt
     5161 tggcaaaaaa attcagatga tgatactgac attaacgtga tcaaatcatg cctgcgacaa
     5221 gaatacaatc tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta tcgttatata
     5281 gaacaagtgc cacgcacagg tgtaaaaaaa cctgataccg aacaacttaa cttgcttaaa
     5341 aaatcacagc gagcctggct ggattttagg gacaaagaat gtgaattaat cctttcaaat
     5401 gaggacgttc aggatttaat gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     5461 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     5521 ggccagcccg agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     5581 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     5641 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgacccc
     5701 cgcggcggta aaaatcacca gcgccggaat ttgtgccacc agaccatcgc caatggtcaa
     5761 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca cgccgaccag
     5821 caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat cgccgcgaac
     5881 aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca cttcggagcg
     5941 gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt caatcgccat
     6001 ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga tacgcccggc
     6061 acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca cgataccgat
     6121 agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc ctgccgccgc
     6181 cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa gtgccagacg
     6241 caacaacgta gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg tgcgctgggt
     6301 aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa acaacaggtc
     6361 gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga tcaaaatcgg
     6421 tccggcaagg atctgccatt gtgtcgattt caggtttgcg ggcaggcgca gcatcgcggc
     6481 cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc accggaagat
     6541 gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt tgccagaccc
     6601 aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga ccaatctccg
     6661 catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg ttattttcag
     6721 cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc actttcggtg
     6781 cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg ttattgacaa
     6841 tgacatcagc tttcggcaca tcggccatca tccgacgccg tgcagcagct cgctgcatct
     6901 gacggatccg ccctttaaca tgagggtcac cttcgctttg tttgaactca tcacgaatat
     6961 cctgccgcga catacgcagc tttttcaggt ggctgaagat ttggaaaaag acgtcaaatc
     7021 ccaccattgg aatgacacca agcaccacca gcagtgcgca tagcccaacc agatccatcg
     7081 cattacccat ggcggtagtc ggagactcgg ccattaagcg catcatctgc ggccagtgat
     7141 gccagagata aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt gccttaagca
     7201 actccgcgcc agtctgtgcc gagaacatcc gtttaatgcc cggtagcggg ttgagtttgg
     7261 aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc ccccagcatg accggagaaa
     7321 tgagcgccac cagcaccacg ccgctaatca gcggcagcag cgccagcatg gcttctctga
     7381 tcagcagaat aatctgcccg aggatcagat tcgggtca
//
LOCUS       DAJTVJ010000181         7341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000181 DAJTVJ010000000
VERSION     DAJTVJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.181"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..489)
                     /gene="mug"
                     /locus_tag="OO448_003921"
     CDS             complement(<1..489)
                     /gene="mug"
                     /locus_tag="OO448_003921"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6339011.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QAL"
     gene            614..689
                     /locus_tag="OO448_003922"
     tRNA            614..689
                     /locus_tag="OO448_003922"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:647..649,aa:Ile,seq:cat)
     gene            complement(743..1507)
                     /gene="nfeF"
                     /locus_tag="OO448_003923"
     CDS             complement(743..1507)
                     /gene="nfeF"
                     /locus_tag="OO448_003923"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR6339012.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1795..2418
                     /gene="nfeR"
                     /locus_tag="OO448_003924"
                     /gene_synonym="yqjI"
     CDS             1795..2418
                     /gene="nfeR"
                     /locus_tag="OO448_003924"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6339013.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2525..4045)
                     /gene="aer"
                     /locus_tag="OO448_003925"
     CDS             complement(2525..4045)
                     /gene="aer"
                     /locus_tag="OO448_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6339014.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4463..5842
                     /gene="ygjG"
                     /locus_tag="OO448_003926"
     CDS             4463..5842
                     /gene="ygjG"
                     /locus_tag="OO448_003926"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6339015.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5884..6216)
                     /locus_tag="OO448_003927"
     CDS             complement(5884..6216)
                     /locus_tag="OO448_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR6339016.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6435..>7341
                     /gene="ebgR"
                     /locus_tag="OO448_003928"
     CDS             6435..>7341
                     /gene="ebgR"
                     /locus_tag="OO448_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6339017.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYE"
BASE COUNT         1796 a         1979 c         1835 g         1731 t
ORIGIN      
        1 cagcgcctgg tccagctcgc gatacgcttc aaccagtttc tctagtgaaa cgcgacttaa
       61 accgctggga tttggcagca cccaaatctg cgtcgaacca atggtgagcg tttgtttccc
      121 ccactgtgca ccgcgctggc tgaatccctg ttcatatgct tgtttgccca gaatcgccaa
      181 cgcctgcggc tgataatctt caattttttc aatcagctta cgcccacctg cgtgcagctc
      241 ctgctttgaa acttcattgg cttgcaccgt tggacggtct accagtttgg tgacgccaca
      301 acgataatcc agcagatgct gtgcctcctg cggcttcaac tgacggtcgg taaacccggc
      361 ctgatatatc accttccaga agcgatttgc tggatgagca aagggaaaac cagtcccggc
      421 ggatgaaagc ccagggttga taccgcaaaa tacgacccgt aaccctggag ccaaaatatc
      481 ctcaaccata tttactcctt tctaacatca ccaggaaaag tataacggat tgaaaataca
      541 ttgtttataa aaacagcagg cgcacggtaa tggctggatt gcgacacgga gttactttat
      601 aatccgctac catggcccct tagctcagtg gttagagcag gcgactcata atcgcttggt
      661 cgctggttca agtccagcag gggccaccag atatagcaaa ggctgacgag aaatcgtcag
      721 cctttttctt tttatatatc agttactttg cgtgccagta agccgctgca cgtacccgct
      781 gtgggtcata ctgttccgct tcaaagcggc ggcttaaatt cttaacgact ttaccttcgc
      841 cggttatcca gatgaagtaa tcatcggcag ggatttgcat ctgcgccaga cgcgcatcta
      901 ccgcctgctc atcatgtgcc agccattcga tattaaaacc atcaaggtgc gcgagataat
      961 cctgacaggc gttatcccgc acgctaacca gcgcactaac ttgcggttta acggcaagtt
     1021 tgctcaacgt ttccaagcgg cggcgcagtg cgggcattcc ggattcatcg cagacataca
     1081 gctgatacgc gtaatcttcc ggcaccacca gcgaaccgcg cggacctgca accgtaagtt
     1141 tatcgcccgg ttgcgcctgc atcgcccagc cgctggcgac cccaccgtcg tgaataaaga
     1201 aatcgatcgc cagttcatgg cgtagttcgt catacagcgg cgtatagtca cgcgacggtg
     1261 ggcgtggtcc ttccggccag acgatgccct cttccgttac cgttggcggc acaaagtgag
     1321 cgtcaggttg aggaaagaag agtttgctgt gatcgtcaaa gccgcgcgat gtaaaaccgt
     1381 ccagcgcctc gccgccgagg acaatgcgct gaaaaccggc gctgatgcgc tcaacgcgta
     1441 acacagtcag ttcacggaag cgcagatcat tgcgaacgcg ctgcgggtag cggggggagt
     1501 tattcattgt tatcgccttc gtgatggtaa tcagatatat ctaaataatt ttcgtaaatg
     1561 ataatgattg ttaatcatga taaatgcaag cgatttgtag aactgatatg tctatagtct
     1621 gataagacga accgcctctt ctcaggcatc attactcaac gccggatgcg gcgtgaacgc
     1681 cttatccggc ctacgtgtga gatgagtcca catttaaaaa tcatttttat tcttgcaaga
     1741 gtgctcatat cagatataaa ttagatatat ctaatcaagc aaaaggaggc tgatatgagc
     1801 catcatcacg aagggtgttg taaacatgaa ggccagccac gccatgaggg ctgctgcaaa
     1861 ggtgagaagt cagaacacga gcactgcgga cacggtcacc agcatgaaca cggtcaatgc
     1921 tgcggtggtc gccacagtcg cggcggcggt cgtcggcaac gtttctttgg tcacggtgaa
     1981 ttgcgtctgg tgattctgga tattctctcg cgcgatgaca gccacggtta cgaattgatt
     2041 aaagcgattg agaatctaac ccaggggaat tacaccccaa gcccgggcgt catctaccca
     2101 acgctggatt ttctgcagga gcagtcgctg attaccatcc gcgaagagga aggtggtaag
     2161 aagcagattg cgctgaccga acaaggcgcg cagtggctgg aagaaaaccg cgaacaggtg
     2221 gagatgattg aagaacgcat caaagcgcgt tgcgttggtg ctgcgctgcg ccagaacccg
     2281 caaatgaagc gggcgctgga taattttaaa gcggtgctgg atttacgcgt caaccagagc
     2341 gatatcactg atgcacaaat aaaaaagatc attgcggtga tcgaccacgc cgcttttgat
     2401 attacccaac tggattaatc accgattgcc ggatgcggcg taccctacac accccgcagg
     2461 cctgataaga tgcgccagca tcgcatcagg cattgtgctc caaccgccgg atccggcata
     2521 ccgattaatg cagcaccgtc accgcgtctt ccagtcggct ggcgcggtgt ttcaccatcg
     2581 ccgacacctg cgcactctct tccaccagct cagcattttt ctgggtgatc aggttaagct
     2641 catccactgc acgggtcagg ctggaaagcc catcggcctg ttccagcgtt gaatggctaa
     2701 tctgggcgat caactgggtg acgtttttca cctgtgccac aatatcttcc atcgtccgtc
     2761 cggcggcgtg tacctgctgc gaaccggatt gcaccttatc agcactggca tcaatcagct
     2821 tgcgaatatc gttggcagca ttagcgctgc ggctggctaa atgacgcact tcccctgcca
     2881 ccactgcaaa acctttgccc tgttcgccgg cacgcgccgc ttccaccgcc gcattcaggg
     2941 ccagaatatt ggtctgaaac gcaatatcgt taatcagcga agtaatggtg ccaatgcgct
     3001 gggtactgtc ggcgatatcg tccattgtct tgatcaccgt ggtcatcgcc tccccaccct
     3061 gcactgccgc attactggcg gtgatagaaa gtttatcggc agccgacgcc gtggcagagt
     3121 tctgtttcac cgacgccgcc atttggttca tggtcgccac cgtttgctga acgttatcaa
     3181 ctgtctgctg ggtatgttcg ttcagttcat cggtgccttt cgccagcgtc tcactgccat
     3241 ttctgacact ggacacctgg cttgagacat cgttaattag ccaacggcac atcaggccaa
     3301 gctgccctac cgcgcgtaat gtcagcccca gctcatcgct gcgattcagg tgctcaacac
     3361 tattacgctc tccggtcgcc accttcagtg cctgacgggc gacattttct atcgggcgca
     3421 caatctgcca ttcaaaacaa gcgcttgcca acaataccac taacacacag aggaaatacg
     3481 tcaccaccgg ggcagcaaca aaccaaagca tggccgccag caagataaac atcagggtca
     3541 tcactccacg cgcccgccag cgaagcggta atgaaggcag tttacccagc cagcctttac
     3601 gcaccaccag gcctttatga atacgcttac tggtacgtcc ggcgttcagc gctttgtaca
     3661 gcggctccac cgccgcaatc tcttcatccg tcgcccgggt acgaatcgac atatagccac
     3721 tgatttttcc ctcgcgcacc atcggtaccg cattggcccg cacccaataa tggtcgccat
     3781 ttttgcggcg atttttcacg atgccgctcc agggctcccc ttttttcagg gtgaaccaca
     3841 tatccgcaaa cgccgctttt ggcatatccg ggtgacgcac catgttgtgc ggctgccctt
     3901 gtaactcttg caaggtaaag ccgctcacct gcacaaaagt gtcattagca tgagtgatat
     3961 agctttgcag atcggtagtg gacatcagag tggtatcgtc cgccagcggg gtattttgct
     4021 gggtgacata cggatgagaa gacatgttcg cgtcctgtgc aggttatatg gttgttaact
     4081 tcttgtcaga gtttatgtcg gccccgctgc ggttatcttt aaccgattaa tttgatttag
     4141 atcgcaattt gcgattaaag cacaaatcta attctttgat ttaaaatact ttcactctgt
     4201 tactatacga aaacgttaat tatcttgccc aaaaatcagg caattattgc cctgaaaacg
     4261 tgcatttgcg cagcaatcat caaatccata cccgacaaaa accgtgcaaa ataacaacaa
     4321 atgttaacag atagcattaa atattgcaca aatgataacc gaatttgtgt ttatcccgat
     4381 tttcgcgatc gcagccggag tggcgcaatc cctgcaatac ttaaatcggt atcatgtgat
     4441 acgcgagcct ccggagcata ttttgaacag gttaccttcg agcgcatcgg ctttagcgtg
     4501 cagcgcccac gccctgaatc tcattgagaa gcgaacgctg gatcatgagg agatgaaagc
     4561 acttaaccga gaggtgattg aatacttcaa agagcatgtc aatccggggt ttttagagta
     4621 tcgcaaatct gttaccgccg gcggggatta cggagccgta gagtggcaag cgggaagttt
     4681 aaatacgctt gtcgacaccc agggacagga gtttatcgac tgcctgggag gttttggaat
     4741 tttcaacgtg gggcaccgta atccagttgt ggtttccgcc gtacagaatc aacttgcgaa
     4801 acaaccgctg cacagccagg agctgctcga tccgttacgg gcgatgttgg cgaaaaccct
     4861 tgctgcgcta acgcccggta aactgaaata cagcttcttc tgtaatagcg gcaccgagtc
     4921 cgttgaagca gcgctgaagc tggcgaaagc ttaccagtca ccgcgcggca agtttacttt
     4981 tattgccacc agcggcgcgt tccacggtaa atcacttggc gcgctgtcgg ccacggcgaa
     5041 atcgaccttc cgcaaaccgt ttatgcagtt actgccgggc ttccgtcatg tgccgtttgg
     5101 caatatcgaa gccatgcgca cggctcttaa cgagtgcaaa aaaaccggtg atgatgtggc
     5161 tgcggtgatc ctcgaaccga ttcagggtga aggtggcgta attctgccgc cgccgggcta
     5221 tctcaccgcc gtacgtaagc tatgcgatga gttcggcgca ctgatgatcc tcgatgaagt
     5281 acaaacgggc atggggcgca cgggcaagat gttcgcctgc gagcatgaga acgtacagcc
     5341 ggatatcctc tgccttgcca aagcgctcgg cggcggcgtg atgccgattg gcgcgaccat
     5401 cgccactgaa gaggtgtttt cagttctgtt cgacaaccca ttcctgcata ccaccacctt
     5461 tggcggcaac ccgctggcct gtgcggcggc gctggcgacc atcaatgtgt tgctggagca
     5521 gaacttaccg gctcaggctg atcaaaaagg cgatatgttg ctggacggtt tccgtcaact
     5581 ggcgcgggaa tatcccgatc tggtacagga agcgcgtggt aaagggatgt tgatggcgat
     5641 tgagtttgtt gataacgaaa tcggctataa ctttgccagc gagatgttcc gccagcgcgt
     5701 actggtggcc ggaacgctca ataacgccaa aacgatccgc attgaaccgc cactgacact
     5761 gaccattgaa cagtgtgaac tggtgatcaa agcggcgcgt aaggcgctgg cggccatgcg
     5821 agtaagtgtc gaagaagcgt aatatcaaat cggatggcga tacgacgtcg ccatccgatc
     5881 tttttatacg acgcgtacgc ccgcaggcat catccgctcc ggcgttaaca gcacgctttc
     5941 actgccgtca tcggtttccg cacacaacag catgcattcc gacgtttcac cacgcatttt
     6001 cgctttttgc agattgcata agaccaccac cgtttttccc atcagctcct cttcgctgta
     6061 gtacggcacc aggctggtca cggtttgcag tgttttttcc cccacatcaa cctgtacgat
     6121 gtacagcttg tcggcgtttt catggcgttt cacttccaca atctttccga cgcgcatttc
     6181 cagacgtgca aaatcagcgt aagccacggt ttccattgct tcctcccttc aagtaaaatt
     6241 ttactaaact atagcaaaat tttttctcaa tcctgtaggc taaaaatgga gaatgcaggc
     6301 gtgatcacat tcgtaagccg ctgtgttacc gttacagcgt caaagaaacg cgctttattt
     6361 actgaaaaca ggtgacccga taagcacttc ctctacaatg ggggcgcaca tcagggaaag
     6421 taaaaaaggt aaacatggca acattaaaag acatcgcaat cgaagctggc gtatccctgg
     6481 cgacagtatc cagggtctta aatgacgatc cgacattgaa tgtgaaagaa gagacgaaac
     6541 atcgcattct cgagatcgcc gaaaagctgg agtacaagac cagtagcgcc cgtaaactac
     6601 agacaggcgc agtcaaccaa caccatattc tggctatcta cagctaccag caggagctgg
     6661 agatcaacga tccttactat ctggcgatcc gccacggcat tgaaactcag tgcgaaaagc
     6721 tggcgatcga actgaccaac tgttatgaac acaacggatt accagacatt aaaaacgtca
     6781 ccggtatttt aattgtcggc aaacccacgc ccgccctgcg cgccgctgcc agcgcgttga
     6841 ccgacaatat ctgttttatc gactttcacg aacccggcag cggttacgat gcggtggata
     6901 tcgatctggc acgcatcagt aaagaaatca tcgacttcta tatcaaccag ggcgttaatc
     6961 gtattggttt tattggcggt gaagatgagc ctggcaaggc ggatattcgt gaggtcgcct
     7021 ttgcggaata tggccgactg aaacaagtgg tacgcgaaga ggatatctgg cgcggcggtt
     7081 tttccagttc gtcgggttat gaactggcaa aacaaatgct ggcgcgggaa gactatccga
     7141 aggcactgtt tgttgcttcc gattccattg ctatcggcgt actgcgggca attcatgaac
     7201 gtggcctgaa catcccacag gatatttcgc ttttcagcgt taacgatatc cccaccgcgc
     7261 gatttacctt tccgccgctc tccaccgtgc gcatccattc cgagatgatg ggaagtcagg
     7321 gcgttaacct ggtgtatgaa a
//
LOCUS       DAJTVJ010000182         7213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000182 DAJTVJ010000000
VERSION     DAJTVJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.182"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..135
                     /locus_tag="OO448_003929"
     CDS             <1..135
                     /locus_tag="OO448_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6339018.1"
                     /translation="DVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRFER
                     "
     gene            complement(488..1228)
                     /gene="phoN2"
                     /locus_tag="OO448_003930"
     CDS             complement(488..1228)
                     /gene="phoN2"
                     /locus_tag="OO448_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6339019.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(1557..2423)
                     /gene="ospB"
                     /locus_tag="OO448_003931"
     CDS             complement(1557..2423)
                     /gene="ospB"
                     /locus_tag="OO448_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6339020.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            complement(2673..2792)
                     /locus_tag="OO448_003932"
                     /pseudo
     CDS             complement(2673..2792)
                     /locus_tag="OO448_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3036..3408
                     /locus_tag="OO448_003933"
                     /pseudo
     CDS             3036..3408
                     /locus_tag="OO448_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3970..4917)
                     /gene="icsP"
                     /locus_tag="OO448_003934"
     CDS             complement(3970..4917)
                     /gene="icsP"
                     /locus_tag="OO448_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6339021.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIDYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(6299..6451)
                     /locus_tag="OO448_003935"
                     /pseudo
     CDS             complement(6299..6451)
                     /locus_tag="OO448_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6507..7199
                     /gene="ospZ"
                     /locus_tag="OO448_003936"
     CDS             6507..7199
                     /gene="ospZ"
                     /locus_tag="OO448_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR6339022.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYGK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RLSQIKARMPVT"
BASE COUNT         2204 a         1391 c         1365 g         2253 t
ORIGIN      
        1 gatgtaaaca aaaaatttca aaaagcaaat agtggggaca ctatgcttga caatgcaatg
       61 aaaagcaaag attcaaaaat gattgatttt ttattaaaaa atggagcggt atcaggcaaa
      121 cgatttgaga ggtgacctaa aatatattta ggttgtgccc tgaatctgaa gttgtcattt
      181 ctgttatatt gatcgacata ggggatggtg cagaccgttc ccctgcaaca ccacattgca
      241 tgtggtgaac ggaaccgccc cgagtttcct agagaatgtt ttatctgtga actcaggttg
      301 ccagatcatc gtttccgatg gcagcataat aagctttttc tgcttctgcc ggagggatat
      361 gtcccagcct ttccagtaat cccttaaatt caggggtaaa acgtggtgtg cccatagact
      421 cctcctatgc tcatataggg tagatttgtc tatgggccct ccatagcctg atacaggctg
      481 tccagcttta tggggtcagt tcattggtag gagtatttaa ttcttcaaac tcttttttgg
      541 cttcgctaag gcttttggta aattcaggtg tattatgaag tactgcaaca accgatgctc
      601 ccattaaacg cccagcctct acatcgcttt gccaatgcgc accgcagatg acccgacttt
      661 ctccaaactc atatccacgt cgaagtattt ccgctttacg ttgaggatta atctccgcaa
      721 gtatcagtgc tactgcccaa ccaaaggatg catgaccaga gggataagag ccagtgatag
      781 ccatgttctc atctttatca ggtgtacagg ttgcgtcttt ataaataacg aatggacgaa
      841 ctcttttata atattctttg gcgttacgca cggcgtaatc atggctatcc tgtagtactt
      901 gtgttaacaa ctgatataag attggggtat tttcccttga aatatccatg ccataagcat
      961 ctgaaaatgc aagaccaaaa ttctcgtagt atgcatcttc gctagcgaga cgtacacgat
     1021 tagcatcccg gagcgagcgg ccgaattcat aatgagcttt gtcagcctga aatactactg
     1081 aatcctctgc cggaggcggc ggaagtattg acaaactgtc tggtgaagtt tgttgagtga
     1141 gaaaaccttc tgccttcaga gcatttgctg aggggataaa aatcatattt gtagcaatac
     1201 aaaaaagaag aaagtttttg gttttcataa tttatttcct tagcataatt aattgatgat
     1261 tttggtcagc tgtaaaaaac gagctgtgtt acaaaaaaca caagtcatgc ggaaaaattt
     1321 gaacagagtg atggaaaaac aaaaaatagt cttatattaa agtaataaca ttatttttca
     1381 tattcacgaa aaataatgtg gtaaaagaat gatgtttttt atctatttgt tcgtatcgtt
     1441 ttgtctctgt attatgatga tttgtgattt tccctcatag ggaagcactt ttctactaaa
     1501 gataaaataa cttcatctta aatgcaaaca attaactata ggttaaaaat tttatactaa
     1561 tccagttctt tattaataat aaaagtcttt ttctgagagc ttctttttac tgtatgctcc
     1621 ggatcagcag gaattgaagt tgaacgataa tgtgagtagg gaaaaagctc ttgaccatag
     1681 tgaactccat atccatgata gccggatatt tttagaccat cactcaatga ctcatcgttt
     1741 tcaaggtgtt tttttatctg ctctagcaaa gattcttttt ccttaaaaaa aggcagagag
     1801 ttaaggatac aagaaagact ttcagcaggg gcattgttaa aatttttagg agccacttta
     1861 tcagcggagc cgcatgaagt aaaacggata tctttcacat ttagaatacc acagtctttc
     1921 attcttgaaa tgattgtttg tacatcaata agttccgaac caaggcctaa ttgatgagaa
     1981 ccaggactac cgtgcccaag aatataaacc ttattatact ctttaaaatc agtatcgttt
     2041 actgttttta ttttcgcttt ttttacagaa aaataagcag cagcattcaa tgcgtcatca
     2101 tcattattta ttactattgg ttcactctgt ggtagccatg attcagccat ttttagaaaa
     2161 tcatttttac ttttttcatt tttaaaaaca taatcatata acatcttgtt cttaaatact
     2221 cttggtattt gttgtcccat aatagataga acatcattgc tatcacaaaa acctttctct
     2281 cctaaaaaaa ccaatgctgc ttttgggtga gtacatgatg aattttttac cctttttatt
     2341 atatgtgcaa atgcaatatc tgatatgctt tcattctttt tattgactat tctacagtat
     2401 ggtctaacac catctaaatt catacctaca tgactccata tattgtttta taaacatcat
     2461 agtaaagggt gttatatttt catatagtat gcaagcttct ttgtatacta tgatttaaaa
     2521 aaacggaaca aaaatgcaat tatattctgg cattgtttaa tgctgtttgt cttttttatt
     2581 cattatttca atgaaagaat ttcaactaca aagttaattg atcctaccta cataatatag
     2641 acacagtacc taaagtaccg tggagatcat ccggggaagc cccgggtctg ataccagtgc
     2701 aggtagaccg ctatcttgtt ccggtaatgt tctgccgttc tttacggcat cacctctagc
     2761 agaaacatcg cgtaactgcg ccaagtatga tgaacttctc ttaccgctgc gatctggttc
     2821 aacaatatgc agatggtaca caacctcttt aaccagccca tccggattat tcagagccgc
     2881 ttccgcaatc cggaataaaa gtgctgtttt accgtgaacc ctctgaaaat cagcgagcag
     2941 tgttttaacc agccgacgtt cagagcgtac tgacagacgg tggacaattt gaataaacag
     3001 ttcaatcaga ccgtagatgc tctcccgttg gcgacatgct gggagcggtg gaacaccgct
     3061 tcggcaacga gcttccggcg tctccagtga agtgactgac ggataatggt tcatgctacc
     3121 gggctaatga aacacgccag ttcacccgga tgttgggact ttgttgaacc gaagaacacg
     3181 gcggtgcgga gtccggagag taacagaata gcaaagagct tcgtgaaaac aataaagtgt
     3241 gactacatca gtatcatgcc caaacctgac gggttaacgg cagcaaagaa ccttgcagag
     3301 gcattcgagc attataacga atagcatccg catagtgcgc tggattatcg ctcgccacgg
     3361 gaatatctgc ggcagcgggc caatgataac aggtgtctgg aaatataggg gcaaagccac
     3421 ccacgaacga tccagcatat aatcaagaaa ttagatttca tttaacataa gatagattat
     3481 gtgcaccaag aggcgtttcc ttaatttctg aatttacagg ctcttttgct ctctttgcta
     3541 tgctgtggat ttggaaagat agagtgctgg ctgattattt aggtattttc atcagcatgt
     3601 ccctgatagc actgttccat cagcgctttc aggcgataat agtagacctt gggtagcctc
     3661 tgcccatgtg taacttgata ccccgagaac agccacgatg atctaagcgg ttcttatttc
     3721 tccggtctca gggtcggtaa tgccaacagt aagtccacag taatcaacga agagtttttt
     3781 gaaccgcccc ggaaatcctg gagactaaac gccctgagaa agaggtaaac agaatgacta
     3841 aaaatacacg tttttccccc gaagtccgtc aacgggcagt tcgtatggtt ctggaaagtc
     3901 agggcgaata tgactcacaa tgggcggcaa tttttagcca tgaaaacgta atcaactcgg
     3961 tgaaccaatt caaaaaatat actttatacc tgcggaagtg agaaaaccaa tatattcaat
     4021 acttgcacta tttttaatgg tgccagatat attggtactt tgctcataaa gagatgtatc
     4081 acctttttta ttagaaatgt aatttctgga gccttctata taaaattttg cactgggggt
     4141 gacatagtat ccaatattcg cagcaacaga gcagtaattt tggtttttta tttcatcttt
     4201 aaaaattgtc tcagtctgat agtgtttatc tgtatcagat gaacgaaccc aaccactata
     4261 tttcagttct gctccaaact caaaattctc atggcggtaa ttagcagtta atccaatata
     4321 aggtattttg aagagctgtt tgtaatctat tgttctttca ccactaggat gtgccccttt
     4381 tttattcctg ttccccccgt tttcactata aatataactc cctcccattg cattaaaact
     4441 gtaacgactc tcttggtaac ctgctattag tccaagtcga taatccaaat tatttaataa
     4501 ccaaccttta agattcagct cgaattcgtt agcatcacgg agatgcgtat tgggatgcca
     4561 gctctggtcg gtccatacct gaggggtatt ggaattgttc cagtctttat caaccatgct
     4621 tgctttctgg ttacctaaag tagtccaacc ggaaactccg aaagacactt ttggaattag
     4681 cttccaatcg atgccacctc taacaatagt cgcattactg tatttccagt ccagttgact
     4741 aatttttctc cccccttcct tgggatgata aacgcgttct tttgttttgc cactcagaga
     4801 tcctagacta atatccgtac tgatgttgtc cggtatgaaa agtggatagt tagtggtagc
     4861 atgtgtagta aagatcgctg gtacacaaag tgcaagtaca aggaatttta atttcatcga
     4921 gaactctact tttttggttg aaatgtccat gatcttctta ctttataaga taaacgtcaa
     4981 tgccaagcat tattttaatt aaatatttgt ttatgttacc aagaacatat cttttattat
     5041 aatatttatt gaacttttgt aaaaataaaa aaaatacctc attgtgcgaa taaagtaacg
     5101 ggggccatag atttctctac ctaccttttg gtgttcatac ctgaggctga tagtttcagg
     5161 attccgcgac aattctactc accttcttgt tctctcgcca atatgaaatc aatcagaaaa
     5221 gcgttccata agaacaacga taagacaata aattgtcatc acggctgcaa tagccaacgt
     5281 gcctttcagt accaacatga caacctcctg tatttgacgt aacgtgtgct gataaatatg
     5341 tatttgtatc tcaactgact caatacaaca cttaagtata gtttttcaga ttttgttttt
     5401 cccgtgaaat attatggaaa tggcagaagc aatacattga attccgattc gagtgaatac
     5461 ggctagcggg cacctcactt tagcactgaa gccatcaact tttatttgac ttatttcaat
     5521 ggaaagcttt ctcactatca gtacaggtag ttcccatcct tttaaagtcg agagtcagac
     5581 tgcgactaat acagttaaag tgaccaaact cagttatcct ctacacattt tacctcatgt
     5641 ttacttggac aattcattgg tgcttttata aaagaataaa tattctacta atttaatgca
     5701 tcacctaaaa tagtattttt tataatagaa ctgtatctat agtatattat taaagcttaa
     5761 ttatttatta tgctttaatg tggttgtccg attaaggact aatccttgaa aacgccgtat
     5821 agggcgggag ttcactctat tgcgataacg taaccgtaca atattgtgcg tttcccaaat
     5881 tgaccactac tgaactattc tgggatttaa atgcaataaa atggttggtt gaaggtcgtg
     5941 gtgatgatgt tagtcctttt gagaatattc cagtgccaga tcttacttat aataaagagg
     6001 cttggcagtt tggataagct attgtcctta ttccactaat acaaactttt tgtaaagtat
     6061 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
     6121 ataaaaggaa gagggagccc caattaagtt atcaagcatg tttatgatac ctgcagggta
     6181 taaattttct ggctgcaagc agacacatat aaaacgtctt tattgatcct acccacataa
     6241 tatggacgca gtcgtaagtg aggctctgtg agcctaccca cgtaatatgg acacaggcct
     6301 aagcgaggtt ctggttttca aattgttccg gactgaggcc gccacaccaa cggtgccgtc
     6361 gccagcgatt gtaatcgcac tcgatataat taaacactgt tgcccgcact atttcccgat
     6421 agataaagtg ttctccgtgg agacattcca cttaggaaca attatattgt tcatggtaat
     6481 gtttatattt gagtatagag attaaaatga ttagtcccat caagaatatt aagaacgtat
     6541 ttccaataaa tactgcaaat actgaatata tagtgagaaa tatttatcct agagttgaac
     6601 atggttactt taatgaatca ccaaacatat atggcaagaa gtatatctct ggtataacca
     6661 gaagtatggc acaattaaaa atagaggagt ttattaatga gaaatccaga cgacttaatt
     6721 atatgaagac aatgtattct ccatgtccgg aagattttca gcctatttcc agagacgaag
     6781 ccagtacacc ggaaggaagc tggttgacag ttatatctgg aaaacgtcct atggggcaat
     6841 tttctgtgga tagtttgtac catcctgact tacatgcatt atgtgagctc ccagagatta
     6901 gttgtaagat cttccctaaa gaaaatagtg attttttgta tataattgtt gtattcagaa
     6961 atgattctcc ccaaggagaa ctacgggcaa atcggtttat agaactatat gatataaaaa
     7021 gagagattat gcaagtgtta agagatgagt cgccagagtt aaagtctatt aaatctgaga
     7081 ttattattgc tcgtgaaatg ggagaacttt ttagttatgc atcagaggaa atagatagct
     7141 acataaaaca aatgaatgac aggctttctc aaatcaaggc tcgaatgcct gttacttaat
     7201 gtatattaat tgt
//
LOCUS       DAJTVJ010000183         7134 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000183 DAJTVJ010000000
VERSION     DAJTVJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.183"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..4263)
                     /gene="traI"
                     /locus_tag="OO448_003937"
     CDS             complement(<1..4263)
                     /gene="traI"
                     /locus_tag="OO448_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR6339023.1"
                     /translation="MMSIAQVRSAGSADNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVTNVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKRKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     MYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     VGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLERVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEG"
     gene            4345..4572
                     /gene="vapB"
                     /locus_tag="OO448_003938"
     CDS             4345..4572
                     /gene="vapB"
                     /locus_tag="OO448_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6339024.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            4572..4970
                     /gene="vapC"
                     /locus_tag="OO448_003939"
     CDS             4572..4970
                     /gene="vapC"
                     /locus_tag="OO448_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6339025.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(4979..>7134)
                     /gene="traD"
                     /locus_tag="OO448_003940"
     CDS             complement(4979..>7134)
                     /gene="traD"
                     /locus_tag="OO448_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCR6339026.1"
                     /translation="LPRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCI
                     YWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATV
                     VALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGRDSDIRIG
                     DLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPS
                     IDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAY
                     LMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVK
                     AIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIR
                     GLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYG
                     EKAAATLFDVMNTRAFFRSPSHKIAEFAVGEIGEKEHLKASEQYSYGADPVRDGVSTG
                     KDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEM
                     ENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQQPQQ
                     PVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
                     QENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         1484 a         2201 c         1827 g         1622 t
ORIGIN      
        1 accttccgtg ccttcaagcg cgatggcaaa ggtttcactc gccccctgtg caccgtgggc
       61 ggtgatggca taggccaggt caatatgttg ctctgcccgc tcctgaccgg ggcgaatcac
      121 acgggtctgc tgaccgtcag aaagtgtgac actgtcaccg gaaaccgctg tcaccgtcca
      181 gacgctgttg gccacataac cgcgctcccg gtcactcttc gtgaagcgta tcctgtcacc
      241 ggtccccacc cggatggtgt ccggggtgta cagcgtgacg ccttcagcca ccgcctcccg
      301 gggcgaaatc agccgcgtgt taccttccgc atcctgcagg gttatcagcc cgtcatcctt
      361 gctgataccg gcaatccggt gatacacatt atccaccagg gcaagggcat ccgggttatt
      421 ctcccaggtg gagagacgac gcagctcccc gtcgcgtata ttcgctgtgt tcaggacagg
      481 caccatgacc tgcacctgcc ccagttctcc ggccttttcc cgtgcatcat gaatcatgct
      541 gttcagtacg cgccgatcct cattcaggtg cgtgacaatc agcgtctgct cccgtgcttc
      601 cggtgtcctg ccggtatagt cgcggacaat ggcttcatac agtgtcatgg ggacatccgg
      661 aaaagcctcg cctttcagca tcgccttctg ctgcgcttct gccagtttcg cttcctggct
      721 gtgactgaac tccgtcacgg agtgctccgg tgcccatgcg ccctccagac gtggcacctg
      781 agacggtttc acacgctcaa gcccggacag cgccctttcc acatcccggt taatcaggct
      841 gtataccgcc tcccgcagtt ccggcgtctg acgcacaatc tccttcatga tgaccacatc
      901 ggcagcactg cgcgtctgct ggagacggaa aggctgacca ggcgcgatgg cctgcagctg
      961 gtccgtgtca ccgctggcca cagcccgacc gccaccgacc gcaatcaggg cgtatgcccg
     1021 tgccatgtcg gtattgccca ccattgagct ctcatcgagc aggaacagcg tgttgctgaa
     1081 atccggcgtt tctccgctgc gctgctgcag ctgcgtgtca tgcagaaagg acgccagtgt
     1141 ctgcgcatcc acgccggcgc tgcgcatctc cccgaccgcg cggtgcgtgg gacccagccc
     1201 aacgacacgg ggacgttcac tctccggcag catgttcacg gctgacatca ccgcccggaa
     1261 ctgtgtggtc ttacccacac cggcatagcc ctgcaccacc gtgaaacggt cggacgtttc
     1321 cagtatcata cgggtggcgg cgcgctgtcc tgatgtcagt ttctccatga gttcgccagg
     1381 tactctctcc atcagcggcg tgaccgcctc cttaccttcg agaatatggc gaagaatgct
     1441 cttttctgcc tcatacgacg cacgggaaac cagcaggcct gtgccatagc cttttgccac
     1501 atccacatac agtaaatcac cccgttttat ctgcgcattg atttcccctc ccaggtcagc
     1561 aaaaccggtt ccttctgcag caaacgactt cgcctctgtc agcaggtcca ccatgctgaa
     1621 ggccaggttt ttactttcca gcacaggaag ggccagatga atggcctgct gtgccggcgt
     1681 gtgaagcccg gctttctgca gactgatagc ggtttccagc aatgtctcac cggcacgcgc
     1741 ctttatctgc tcagaaacca ccgtaaagga gggatggcgg gcaagttttt ccgcagtacg
     1801 ggtttcatcc agtgaggaat acagccggac atcacgacca ctgcgggcca gaccgttcag
     1861 ggtggcattg tccattgcca tctgtgtgac ggaggcaaaa accgtcgcac tgtcgctgac
     1921 ggaatgcccg ggcgtttcca cccagccgtt ctccagcttc agtgccgtga acggtgaatc
     1981 gctcacaggc agggtggccg gttcagcccg tcccggcaca acaaccgtca tcgcatcttc
     2041 actgacggat gccacctgca ggcggtcacc gccggagacg cggagtccgg gaattttccc
     2101 tgtcaccctc agacgctcac cgtctgccac cggcattttt tccggccgga acagcgacca
     2161 gctgctgtcc agggaagaaa tacgcaccac ctgcgtttcg ccctgcgcat tccgcagggt
     2221 caggctgtga ctctgcgccg tcacacgttc tgtcacatag cggtcatgac tgcgtgtctc
     2281 cgggttccac tgctccatca ccattcccgg gcggtacata tcccgcagat aacggctccg
     2341 gctgtccagc cagaccggtg acagggcggt catggttacc tccgggtgtc cgagcacgcc
     2401 ctgtgttttc agctcactgc gaatggcctg tgtcagtatg gcctgttccc gtactccgct
     2461 gacctgtgcc acgctctctt ctccggcttt cacgctggcc gcaaaatctc cggccagccg
     2521 ggcatagcgg acattacggt ccggttcact gatgatggtg gccggtcgct gttctccccc
     2581 ctgccagcga tatgtgttca ccccggcatc cttcatggcc atcagtgcac tgcctgtacc
     2641 ggttcgctgc ccgctgtcgg ttatcaggac ctgtacgtta tgacgtgcgg caccatccag
     2701 cagggttaac gtctctttca gggagagttt ttcgccctgg tcgacgataa cggtactgcc
     2761 cggcgtgaag gccatgcctt ccagcaactg acgacgtccg gttatcagtt caccggacag
     2821 ccgttcatcc tgcttcaggt tcatctgcga gcgacggtca gcagcgataa tctgcacctc
     2881 ccgcccctgc tcccgggcca tcatgaccag ttcagccacc cgctcacgct gcccggctgc
     2941 accgccctgc ccggacacaa tggccagcga cggacggtcc tgtgccagaa cactgacggc
     3001 atcgctgtaa ccggccgtcc gggggacact tttctccgga tgtacggtca cccggttctg
     3061 tttcatgatg tcacgactga gtgcccggac tgacagctca tcgagcatat gaatcccgga
     3121 cgtgaacagc cccttctcac gatcgagggg gataagctgc tcacggctga tggcctcatc
     3181 gataccggcg cgtgcccgtt caatcacacc attttccggc ggcagtatgc caaccgtcct
     3241 ggccagcagg tccgtataca taaactgcac tttgcgttca cttaatccgg caatggcctg
     3301 tgtcaccgcc tgctgcacat ccggcccgtc ctgtgaagcg ggtccgggtg tctgcgtgcg
     3361 ggtatatgcc cgttgttccg ctgcgtcacg atatgcccgg atgtcgaacc cggtttcctt
     3421 cagcgtctgc atccactcgg ccattctgac ttcaggatcg acgtgctgtt tggatttacg
     3481 cgtatccagc gccgccacat cccgggattt cagcgaggcg tcttccccga cggcctcccg
     3541 gatagtctgt gagcgtccgg aaaaggcctc caccggtaca cccggcattt cccacatacc
     3601 gtgcttaccg accacttcag tttcatagcc cagcgcctca acctgctctt tccgtttttc
     3661 ccggtagagc ctgccaaagg caatctgatt agcgtacaca ttctcaatga accccgtttt
     3721 ccccactttg tcactgctca gtgtcttcca ttcgccgtta tgctgcgtaa cattagtcac
     3781 caccgcatgc gtgtgtaact gtggttcctg atcgcgactg gtgtcgtggt taaacagtgc
     3841 catcaccaga ttaccggtca gcaccgtttc tgactgtccg tccgtcatca cccgtgtgga
     3901 ggccagtgcc tccacctgac ggacagcaaa atccacggcc tggttatgtg cttcaataag
     3961 acgtttgtcc cccccgagca tggccatcat ggagacactt ttgggagcgg agaaggtcag
     4021 gtcgtagccg ggacgatgcc tgttactgcc atcctgcatg cggcttaaat ccgccccgtc
     4081 cggcagcctg ccctccagaa ggcgggtaaa gacatcctta tcgacactgc cctgcagccc
     4141 cagttgttca gccccctggc cggcccagcg ttctcccatg ctgcccagca catagtaatt
     4201 atccttgtcg gtataataat tatcggcact tccggccgat ctgacctgcg cgatactcat
     4261 cataaacgta tatccctttg acatatcccg gcatcaatcc cacaatagat atacacaaga
     4321 catatccaca taaggaggca aataatggaa accaccgtat ttctcagcaa ccgcagccag
     4381 gcggtcagac tgccaaaagc ggttgcattg ccggaaaacg taaaacgcgt tgaagtgatt
     4441 gctgtcggac gaaccagaat cattacgcca gccggagaga catgggacga atggttcgac
     4501 ggacacagcg tcagcgccga ttttatggat aacagggaac agcccggcat gcaggaaagg
     4561 gagtcattct gatgctgaag tttatgctcg ataccaacat ctgcattttt acgataaaga
     4621 acaaacccgc cagcgtcagg gagcgtttta acctgaacca ggggagaatg tgtatcagtt
     4681 cggtcaccct gatggagctg atatatggtg cagaaaaaag ccagatgcct gaacgtaatc
     4741 tcgctgtgat cgagggattt gtttcccgca ttgatgttct ggattacgac gctgctgcag
     4801 ccacacacac cggccagata agagcagaac ttgcccgtca gggacgccct gtcgggccat
     4861 ttgatcaaat gatcgcaggt catgcccgca gtcgggggct gattattgtg actaataaca
     4921 cccgggaatt tgaacgtgtg ggcggcctga gaactgaaga ctggagctga cctgttcatc
     4981 agaaatcatc tcccggctca acatcctccc cgcgttcccg gtgcacattg atgttcacct
     5041 cttcacgacg ctgcatctgc tgccaggtgt ccggatgatt ttcctgctgc catgcctcat
     5101 aagcggccat atccaccact tcaccggatt cactgatccc gggtggcagt tgctgttcca
     5161 tctcttcttc cggtttcatt ttcagctcct gctcaatccc ccctgccgga acattcacac
     5221 ctgaatctga cttcttatcg ttgatgacag gagacaccgg ctgttgcggc tgttgcggct
     5281 gttgcggctg ttgcggctgt tgcggctgtt gcggctgttc agcctgagtc acgtcttctc
     5341 cggaaacaac ctccgggaca tccggttcga agaggctggc catctgacga ccttctgctt
     5401 cccttgcggc aagtacggca ctcagacggt tctccatttc cgggttgatg tcacgcggaa
     5461 taaactccgg agcgaccttc ggacgttcct gatatttcag tgagagtttg actgccggat
     5521 acggtccggg cagggtgaca tagcaggtca gatccggcag ggactgaatg tcggaataac
     5581 tgaccagcgt ctggcgctcc atatccttac cggtcgatac cccgtcacgt accggatcag
     5641 caccgtagga atactgttcg ctggctttca ggtgctcttt ctcaccaatt tcaccgacag
     5701 cgaactccgc aatcttatgg ctgggagaac ggaaaaaggc acgggtgttc atgacgtcaa
     5761 acagcgtggc agccgctttc tcaccgtaga tatcttccag ctgggcatag gactggatac
     5821 caaacacata acagccaccg aacttacggg cttccggcag gatctccacc aggtccggca
     5881 gtttgtgtaa tgtgggtaac tcatcacaga agaaccacac acgacgatta cggttttctc
     5941 ccattgccag cagaccacga atggcaatgg acagccacat ggagatcacc ggcttcaggg
     6001 aggcatgagt gtcggcattc gacgagataa acagccagcc gtttttctga tcctcccgga
     6061 caccacgcat ccagtcacgg atggtgaagg gctcaccgtt atgctcaatc ccctgcaggt
     6121 aacggatggc tttcacatag ttggtcagca cagcacggat ggaaatcgcc gttttctcaa
     6181 ttttctcttc caccaggttg gccgccggtg aattacgcag gtaggtacgc agtttttcaa
     6241 ttttgatgga aagcagtgtg tccaccagtt tgctgtagct gcggttgggg tcattacgca
     6301 tcagatacgc cgcttccgca aaaatggtac gtcctgaacc ctgccagaac gggtcctctt
     6361 ttgtgcccat cgggatcaga gtatttgcgg tattatcaaa atccggctgt gtcaggcact
     6421 ccttccacaa atcccaggcg gcacagcgtg catccagcgg attcaggatc ttatcgatgg
     6481 agggatcata gtaactttta acaaattccc ctgaacggtc ataaatcacc accatatcac
     6541 cacgctgacg ggcgtagttg gccagacgac ggataacctc cgacttaccg gccccgaccg
     6601 tgccgtgcag acagaagttc tggatttcgg aatcccggat aatcggcaga tcgccaatcc
     6661 ggatatcgga gtccctgccg tcttttttca gcatccgggc aacgtctttc ggattgtctg
     6721 tcagttgacg accacctgtg acttcatttt cgctctgttg tttaccctga cgccccagaa
     6781 tccaggagac aacaaagaag gtaatcaggc aaataaccag tgccacaact gtggccagaa
     6841 cgaatgcaga ccataactgc tcaccgcacc agatcatata tttatcatgc agtacctgag
     6901 cagcgttcat ccggaaggtt ttgccgtaat actgaatctc atataccggc tgagacttga
     6961 ttaaatcccg catgccttcc agcgtggtac accaccagta aatacagcca ttcacaaacg
     7021 tctgccagct tatttttacc cataaaacca gaccaacgag tatccagaaa aaaataaaca
     7081 ggcagtaaag cataatattg gcgatctggc tgaacatgcg gatacgcggt aatg
//
LOCUS       DAJTVJ010000184         6947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000184 DAJTVJ010000000
VERSION     DAJTVJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.185"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..142
                     /locus_tag="OO448_003941"
     CDS             <1..142
                     /locus_tag="OO448_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339027.1"
                     /translation="ENALCHPQPKPPAPPAWAMMPPSNSLQLLDETFSVSGTESSATR
                     QH"
     gene            complement(275..787)
                     /locus_tag="OO448_003942"
     CDS             complement(275..787)
                     /locus_tag="OO448_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6339028.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            982..1746
                     /locus_tag="OO448_003943"
     CDS             982..1746
                     /locus_tag="OO448_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339029.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1697..3100
                     /locus_tag="OO448_003944"
     CDS             1697..3100
                     /locus_tag="OO448_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6339030.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3100..4566
                     /locus_tag="OO448_003945"
                     /pseudo
     CDS             3100..4566
                     /locus_tag="OO448_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4457..5200
                     /locus_tag="OO448_003946"
     CDS             4457..5200
                     /locus_tag="OO448_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6339031.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5204..6424
                     /locus_tag="OO448_003947"
     CDS             5204..6424
                     /locus_tag="OO448_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6339032.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6428..6934
                     /locus_tag="OO448_003948"
     CDS             6428..6934
                     /locus_tag="OO448_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339033.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1842 a         1662 c         1899 g         1544 t
ORIGIN      
        1 ggaaaatgcc ctgtgccatc ctcagccgaa acctccagcg cctccggcat gggcaatgat
       61 gccaccgtcg aactctctcc agttgctgga cgaaacgttc tcggtatccg ggacggaatc
      121 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
      181 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      241 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      301 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      361 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      421 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      481 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      541 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      601 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      661 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      721 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      781 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      841 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      901 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      961 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
     1021 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
     1081 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1141 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1201 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1261 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1321 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1381 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1441 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1501 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1561 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1621 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1681 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1741 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1801 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1861 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1921 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1981 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     2041 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     2101 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2161 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2221 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2281 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2341 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2401 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2461 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2521 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2581 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2641 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2701 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2761 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2821 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2881 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2941 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     3001 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     3061 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3121 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3181 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3241 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3301 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3361 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3421 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3481 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3541 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3601 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3661 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3721 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3781 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3841 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3901 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3961 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     4021 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     4081 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4141 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4201 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4261 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4321 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4381 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4441 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4501 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4561 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4621 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4681 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4741 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4801 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4861 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4921 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4981 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     5041 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     5101 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5161 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5221 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5281 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5341 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5401 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5461 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5521 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5581 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5641 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5701 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5761 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5821 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5881 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5941 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     6001 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     6061 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6121 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6181 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6241 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6301 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6361 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6421 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6481 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6541 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6601 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6661 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6721 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6781 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6841 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6901 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacgaat
//
LOCUS       DAJTVJ010000185         6797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000185 DAJTVJ010000000
VERSION     DAJTVJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.186"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="OO448_003949"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="OO448_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="OO448_003950"
                     /pseudo
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="OO448_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="OO448_003951"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="OO448_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6339034.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="OO448_003952"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="OO448_003952"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6339035.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5493)
                     /locus_tag="OO448_003953"
                     /pseudo
     CDS             complement(4172..5493)
                     /locus_tag="OO448_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(5501..>6797)
                     /locus_tag="OO448_003954"
     CDS             complement(5501..>6797)
                     /locus_tag="OO448_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6339036.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1805 a         1696 c         1552 g         1744 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 tagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagcgtgt tgataagatc aattgccacg ctacgccgat attcagcaat
     5341 atttgccacc gcaaaagcag tatcaataac ggactgtttc aggctataca cccgttcctt
     5401 taccatttga gtgtagatcc cgcaggcaac tgctgaaatc atgataaaaa tcagcagact
     5461 gactgtaagt acgaacttat tgattctgga catacgtaaa ctactctttt actaattttc
     5521 cacttttatc ccaagcggag aatggcatgg gtttctggta cagatacccc tgagcctgaa
     5581 cacatcctaa tttaattaat tcgcgcgcaa cgtcttcgct ctctacgcct tccgcaatca
     5641 ccgggatccg gttatagcgt gccagatcga aaataatgcg cacaattttt cccgcagtac
     5701 cgaaaatatc gccggtaaat tgcgagtcca cctttatgct gtctggatta atttcataaa
     5761 aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat cgatatccgt accccttgtt
     5821 tacgcagttg ttcaatattg agataaaaat gttcattaat tcgctcgatc acggtttcag
     5881 tgatctcaac acagaggctg ttcgccttca ggccattttc tttcatgatg gtggtaaaac
     5941 gctcatgaaa gtgcggttcg tttaactgcc agggcgaaac attaatatgc agtaagaaat
     6001 catccttcag cgacgcacgc tgactaaaat gtctgaattc acccattgcc gttttaataa
     6061 tctgataacc cagctcattg ataaacccga tatcttcagc aatggtaatg aacttcagcg
     6121 gtgaaatgat cccccgctcc gcagaaaccc aacggcacaa cgcctccgcg cccagaatgg
     6181 cgcgggtgtt aatatcaacg atcggttgat agacgaggaa tagttcgcca atttccattg
     6241 catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt ttcgcgtcat
     6301 cacagaaaat aaactcggag aacatatggt cacggatact ggaaatcgcc agtttcagtt
     6361 tttctgcgta actccaggcg ttttccccct gcaaaggttc acaaatggca gacttatgga
     6421 tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt taccatcgct attttacggg
     6481 taaatgtctg tacattttcc cgacacagaa gtaaatagag atcggcattg aatgtcacga
     6541 tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg acgattttat
     6601 tgcgttcatc aacaccaaac accgcttcca cggcttccag accacacagt ttgatgatcc
     6661 ccacagccag atactgatta ttgttggact caataatcgc catattttta ataaaaccgc
     6721 tttcctgatc gtaagagatc agtgagagca atttatttga ccgttcaatt tcggcatctt
     6781 tcatttccgc gatcgaa
//
LOCUS       DAJTVJ010000186         6762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000186 DAJTVJ010000000
VERSION     DAJTVJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.187"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(61..204)
                     /locus_tag="OO448_003955"
     CDS             complement(61..204)
                     /locus_tag="OO448_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HCR6339037.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(302..553)
                     /locus_tag="OO448_003956"
     CDS             complement(302..553)
                     /locus_tag="OO448_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5 immunity protein"
                     /protein_id="HCR6339038.1"
                     /translation="MKLSPKAAIEVCNEAAKKGLWILGIDGGHWLNPGFRIDSSASWT
                     YDMPEEYKSKIPENNRLAIENIKDDIENGYTAFIITLKM"
     gene            complement(621..2291)
                     /locus_tag="OO448_003957"
     CDS             complement(621..2291)
                     /locus_tag="OO448_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HCR6339039.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPDLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2524..4743)
                     /locus_tag="OO448_003958"
     CDS             complement(2524..4743)
                     /locus_tag="OO448_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6339040.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4922..5188
                     /locus_tag="OO448_003959"
     CDS             4922..5188
                     /locus_tag="OO448_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6339041.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARIRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            complement(5402..6328)
                     /locus_tag="OO448_003960"
     CDS             complement(5402..6328)
                     /locus_tag="OO448_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6339042.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
BASE COUNT         1597 a         1889 c         1547 g         1729 t
ORIGIN      
        1 aaaaaaaagc gcgtcagaat cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg
       61 ttactgcgtt gctaatccgg tcagttcagc tgaggatgac ggggatacag tcccgccctg
      121 aacatcgcgg atatagtttg cctgacatgc agcaagaata atggctgcaa gaagcaataa
      181 aataatccct gttatttttt tcatactgtt ccttgtatat atggactcag gcattcatcc
      241 ataaaaacct gaaatccaaa ggttgaaatc atccatgaaa cataaaattc tataacacta
      301 tttacatctt taacgtgata atgaaagcag tgtatccatt ctcaatatca tctttaatat
      361 tttcaatagc caatctatta ttttcaggga tttttgattt gtattcctcc ggcatatcat
      421 atgtccatga tgctgaacta tctatcctga atccaggatt cagccaatgc ccaccatcaa
      481 tgcccaaaat ccataagcct tttttcgctg cttcattaca aacttctatt gcagcttttg
      541 gtgataactt catagaagca tttcctttta attcaaaaaa ggcatcacca ctatcatata
      601 acaccgtatg ttcaaataat ttattggtca tttttatttc cccattgaat tctcgaatcg
      661 tccacccaac ccggatctgt cttatcacta atctgagtaa cctcaccagt acgatcattg
      721 acaacaacat atttaccagg agaaccatac actgttgctg gatcatttcg acctaaatag
      781 tctggaggtg ttttcttcgg actacgctta tcaaaagatg taccagttgc accgttagat
      841 acggtatttt tgatatcatc ctctgtccat cctctttctg gcatttgtcc cctaattttc
      901 tgatctattt ttagatctgg tatctttcct ttattttttg ctaactcatc atttagtttg
      961 ccttcagcat ctcttttatt atcctctttc ttcttcctgc tttccatcgc agtacttaat
     1021 gcagcatcag catctgcctt ctcttttgca gcagcatcaa atgcagcctg cttattattt
     1081 acatccgtct gcgcccgctg ggctttaagc ccggccattt gccacattct gtgaccgcca
     1141 gccattgggt catgtgcaaa tcgttcaaat tgttttattt cagctattgc atcagcaaga
     1201 gttttattcg ctgcatcaag ttcgctttta cgcgaattat aaacctgaac agctttagcc
     1261 tgtcgctcct gatttctggc aacatcttca tttgcctgat tcagctctgc acgcgcgcgt
     1321 tcataatttc gctcagccgc ttcaaccgga tgcgtagcat cccattcctg ctgacggcga
     1381 ttttcttcat cctgacgttg ttttacctgg tcagggctaa gaacatcact cactgaaaca
     1441 tatacggcat tatgaccgct gtccttcggg aatcgaataa ctgcatccct ggtattaccc
     1501 ccctgagtaa atcctgccgg gcgaacatcc ttatcagtat tatttgtaac acctgggctt
     1561 aatgtctgta ctgctggcgt actgttatta actgaaatat tcagaacagg tgcacctgga
     1621 attgatgccg taaaaacacc tggacgttcg gtaggttttg catcaaccac cggaacactc
     1681 atcggaacac ctgaaacaac cgaaatattc tgtcgttcgt cttttacatc atcaacaaca
     1741 cgaacattta cgtttactgt tgccttatcg agaggtaatg aactgacagg tgattcagta
     1801 atatcatctg cgggtaatga cgtcacaatc tttgacatca tattggggtc atctttcgct
     1861 atttgtgatg gcaatacacc atataaagcc accccccaaa gaccaaattt aaacggtcct
     1921 ttcagggcag ccataatatc agcaatagct gccgataatg ctcccgctga aatactgacc
     1981 gccagaccgc cagcacctgg agtggaaagt gccggaaaac caaatgccac tggcgcagct
     2041 actgctgaca gattaccgcc tgttcccgag ccaccaccgg aattaccatt ccccccgcca
     2101 ttaccatgac cggaaccacc tccccagtga atgccgctac cggaaccacc accccacggg
     2161 ttattttccg aactccaccc ggaaccatca gaagcaccac cacctacacc aagcccggtc
     2221 gggccaccat taatgttacc acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg
     2281 ccaccgctca taaaaattcc ctcttttaag cgttaaaaca attaattaca cgattaatac
     2341 tgtacataaa accactggtt ttatgtacag atcaaaaagt acccgtcatc gttttttctg
     2401 tcaagctcag gtggaaaatt ttttagaaat caggagggat aacacttaga aaacgcagag
     2461 cgggccacaa aaaattttgt ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg
     2521 acatcacata ctcatgcccc ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc
     2581 gtcccgcttc cactcatgcc aggtctgatt aaaaccgagt tgacggaaga gataatccgg
     2641 catctcttcc ggtttgtgga cagcagccaa aagcctgtca tgatggcctt tgagccggaa
     2701 ctgcatcagg tgcggttccg gtgggtacgg agtatcctca tcatggttta tagcttctcc
     2761 gtaacctgcc aggcataccc cgttctccgt gatggtgctg actatcagat gactgtaatc
     2821 cggcgacgga agcacaagaa cggggcaaaa ccctccttcc cggcgaacaa acccgacaag
     2881 catttcctcc ggccctttgc ataacaacag attcagcgtc ctgctgtttc cctctctcga
     2941 aaatacaaca ggcatgagtt gttccggtac agcatcagcg gtgatatcca cccggtgcgc
     3001 tgacggcagc ggagaggcta cgggcctgcg gtcgtcgctg aaataaaaat tatccggcca
     3061 gtgatgcggc agcgccggaa aaccgtaaga gggccacccc tggcgcatct gttccagtac
     3121 atgccggacc gtcgcaccgg aatacattct cacacgctca ggccgcgcca tcgtctcatg
     3181 agattctggc attccctcac gtatatccga gaccatcaca gccccgcctg gcgacaggta
     3241 aacattcacc gggtcatgaa cactctgggg gtaatgcgcc agctcatcac cccataccgt
     3301 gaattctccg gctctgttac tcagcatcat gtagcgggtt atcctgcccg ttcccggctc
     3361 tctggcacgc cccagctccg taataacccc gactaatgcc gtttcacggc tcagacggtg
     3421 gttatcatga cgtttcagcc gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc
     3481 ggcttcctcc ctgcgccgct ccgcttcccg tgctgacagt ttttctttca gctctgccat
     3541 tcccggcgca atccacgtat tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc
     3601 ccgttctttc acggccctgt aggtgcggcg aagagattca atctgtttcc cgagtttcca
     3661 gaaacggagt tgtcttttgc ggtattccgg ttcagcccat gtcatcaggg ggatctctga
     3721 cagaagggaa gcgacagggc gtgctttttt gagtccggtc atctcctttt cgagaatggc
     3781 ctgctctttc agcagcgcat tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc
     3841 caggaactcc ggggtcatct cccacgcgtt gccgcattcc tgcggcaacg tggcaatgaa
     3901 ttcccgcagg gtatccgctg aggcattcgc agcgggaatg gtcaggttaa gggcttccgc
     3961 tgcacgacgt gcggcatcgc tgagttcccg tttccgcacg ttctcctgta cctggatgac
     4021 tgccagcctt gtgcgggaaa cctgtatttc caggttaagg ctgttaatca gacggttctg
     4081 ctcaccgcgt tctgtttcca ttccccgttt ttccatcgca caggcagcct ttccgaggtg
     4141 aaggccgggc gctttctcca gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg
     4201 gtagccggcg ttcgcaagcg ctctgttcgc atggtcagcc catgatgcgc gccacgtctc
     4261 ggcgagtgcc cggtcgttcc agtccctgac ctttcctgca aatcccgttt ccccgacagc
     4321 tctcgtggtc agcatgatgt gcgcatgggg attggtccgg tccatgtgat ggaacgccac
     4381 atcggcaatc atgccccgac tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg
     4441 tgcggcctcg cgggaaatct cacggggaat ggcgagttca atctcccttg ccagctggga
     4501 gttccggcgc tgttcggctt tctcgaccgc attccagaga acggaacggt caccacacca
     4561 ggacggtgca ttcgcagggg tgagaatgac cgcatcatca acgccgcgtt tacgggtgta
     4621 atcgtgggtt aatccggtac ggtcatcggg tatgacagag ccggaacggt aggcagcaga
     4681 agcaacggca ctgtagccgt tttttcgcga aatgattttc atgctgagat gataaatcgc
     4741 catttctgcc gtaatgcctt tttgccgttt tcttttagcg tcgcagaccg cacacaatcg
     4801 aaacgaagtg gagattgtat aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc
     4861 agttttgcaa ctaccagtga cgcgctaaaa tgcgattttt caccgcgcat ggagtcacaa
     4921 aatgagcaga acactcgaac agaagattgc cgaagctgag gccagattac agcgcctgaa
     4981 ggcgaagagc cggagcctgg acacggcgca aaaagtcatt gtgggggctg ccatgctggc
     5041 gaggatacgg aggccggaag aggcgcaact gcgtgcgttc ctgctccagt tcctgcgaaa
     5101 ggaagtcaca agacaggccg atgtgaaccg gatacaaccg ctgattaacg agctggagaa
     5161 gctacccaaa ccacctgcga aaccgtagaa cggagaaacc ccggcgcgta agtgccacat
     5221 gcaccaccga cacctttgcg cttcctcgct cattcgctcg ctacgctcgg tcatgagact
     5281 gcggcgagcg ctacaacctc aatcatgcgg aaagccgcac cattgcaggg aggaaacgaa
     5341 aaagccacca aacgccaaca gctcaaaaaa gcgctgttat ctgatatagg ttttatttgg
     5401 tctactctac aagacctcgt tttttcagtt ggtaatacca ggcgcggcta attccaagag
     5461 tctcccacgg tttcaacgtt cgcgcagatg tggcaacagc accacgctta gacttagcac
     5521 ctccaatttt cccgcccttg cgaccacgtg cagcctgaat ttctggcgtg tgcgtatcag
     5581 ccacatactg tgcgaaagtt tccggcgtga agttcctgtg cgtgtatttc gcaatgctct
     5641 tgccaatagc ccgacattcc gccggagaaa gcggaacggg aagcgatacg ttgtacattt
     5701 cgacacgctg gatcacggca tcaagccatt gtgagaatac aggccagccc tgacgaatcg
     5761 ccctgtaagc ccatttgcgc gtcatttcga acaggtggca gtttcgcccc atcccgtaat
     5821 gtttatcgat gctacggcgc gctgaggcgc tcaaatcgag ataatcagcc agttcatcga
     5881 gggtataggc ttcctcgcgc cactccatca ccagccattc caggtggaac ggatttttgc
     5941 aaatcagtcc gctgtagttc acatctgcac caagtttctc acacagcgca cgctctatcg
     6001 cggcagcgta cttcagtgcc ttaaccgacg catcaggcgc ggttctcacg gcgatgttga
     6061 gtgcatagag caggtgagca tgaccgttca cgggattttt tacggtgatg ttcggcgcgg
     6121 gggcgttccg gtcgctccag tcaatcgcag ccccctcgcg gtcaacgtca aaaaccagcc
     6181 agaactgcgc atgaggctgg ttctgctgga tgtaccgcgc aagaagcgca cgccctttgc
     6241 cggagatgcg aagaccaaaa gcgagatcat ccgtgtgata gggacggtgg ggtaaatttt
     6301 cttcgaagta ttgaagcgcg gcgctcatgt gttacgattc cttccaggtt atcgaccgac
     6361 ctcgcccctg tattctcgtg acaatcttgg cggagagaca cgaatttcag gggcgtttgt
     6421 ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc gaaaacccga
     6481 tccatttacc ataactcccg ttgtacgtac cccttaaaac gccctcaatc gctttctgac
     6541 gcaaaaaacg caaaaagaca aggaaacaca gggtaaacat aaaaaaatgc gtcagaatcg
     6601 cttttagcgc gttgcataac tatgcacgaa agcgatgcgc gatcggtttc gtgcgttctg
     6661 aacaccgaac ggctcaaaac gacaagtttt ctccccctgg atggaaagtt ctgcacgagg
     6721 attttagatc ccatctccca catggaattt ttgtgacact tc
//
LOCUS       DAJTVJ010000187         6651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000187 DAJTVJ010000000
VERSION     DAJTVJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.188"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..739)
                     /locus_tag="OO448_003961"
     CDS             complement(<1..739)
                     /locus_tag="OO448_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6339043.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDI"
     gene            complement(880..2913)
                     /gene="metG"
                     /locus_tag="OO448_003962"
     CDS             complement(880..2913)
                     /gene="metG"
                     /locus_tag="OO448_003962"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6339044.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3045..4154
                     /gene="apbC"
                     /locus_tag="OO448_003963"
     CDS             3045..4154
                     /gene="apbC"
                     /locus_tag="OO448_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6339045.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4399..4680
                     /locus_tag="OO448_003964"
     CDS             4399..4680
                     /locus_tag="OO448_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6339046.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4923..5465
                     /locus_tag="OO448_003965"
     CDS             4923..5465
                     /locus_tag="OO448_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6339047.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5545..6219
                     /locus_tag="OO448_003966"
     CDS             5545..6219
                     /locus_tag="OO448_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6339048.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6235..>6651
                     /locus_tag="OO448_003967"
     CDS             6235..>6651
                     /locus_tag="OO448_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6339049.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELES"
BASE COUNT         1757 a         1508 c         1594 g         1792 t
ORIGIN      
        1 caatatcaat ctgttgaagg tgtattatta catcagtagc atattccaga ccgccttcct
       61 ggacgatctg atccataaca tcctcacgca ctttatatct tgtgcatttc atttcaagtg
      121 ccgtgagtac agccattact ggtgatggta actgtgttgt tttcaggccg tcgatctgac
      181 tgatcgcctg ggcgacggtg ttttgccatt ctaaagaaca ggcgctgaag tcaaatgtgg
      241 ctgttttatt atcccgttgc gacacactac aggctaagcg acgtaattgg gtcaatttgt
      301 ctgcttgctg aacaggaact ccgggaaagc ggcgatgact taacgtggtg tgtacaactt
      361 cgggcggaaa aacgacagca atatctttcg ccagccacgg taaaacagca tctgtatttt
      421 tgtcaggcgc acgggtttct gctacgaaag agggttgttc ctgaataatg gtggataagt
      481 ccgctacctt gagagagccc gttaccgttt gggaaggttg caaagaatta tccttcgctt
      541 gctccacata ccccttcttc accttctccg caatcagctt aagctccgct tttgccgctg
      601 ccgcagcatc tgaaaaactt tttatctggc tttgcccttt ggtgccaact tttccccaac
      661 tgatatgcaa ctcgtttccc tgttgctcaa ctgcccagaa tttatgtgat ttttcgtcct
      721 gatagataaa gtgtctcatt ctgcatccct gtaattttat ccagttgaat gcagatgcta
      781 ccagtattta tgcgggttag agagagacaa aatcgcaaga aaacaacttt aggaatttat
      841 aaagaaaagg cgctgtcgat gcagcgcctt gaagagggat tatttcacct gatggcctgg
      901 tttagcacca gcatccgggc ttaacaggaa gatatctttc ccgccaggac cagcagccat
      961 taccatgcct tcggagatac caaagcgcat tttacgcggt gccaggttgg ccaccataat
     1021 ggtgtgacgc ccgatcagtg cctgcggatc cgggtaggca gaacgaatac cggagaagac
     1081 attgcgtttt tcaccgccga gatccagtgt caggcgcagc agtttgtcag aaccttcaac
     1141 aaactctgcg ttttcgatca gcgccacgcg caggtcaact tttgcgaagt cgtcaaaggt
     1201 gatggtttcc tgaatcggat catctgccag tgggccagta actggcgcgg cagctgcttt
     1261 cacttcttct ttagacgctt ccaccagtgc ttcaacctgc ttcatatcga tgcggttata
     1321 cagcgctttg aacggattca ctttgtggcc cagcagcggt tgctggatac catcccaggt
     1381 cagttctgta ttgaggaatg cttctgcgcg ctcggtcagt ttcggcagta ccggcttcag
     1441 gtaagtcatc agcacgcgga acaggtttat gcccattgag caaattgcct gcaggtcggc
     1501 atcgcggcct tcctgttttg ccaccaccca cggagcctgc tcgtcgacat agcggttagc
     1561 cagatcagcc agcgccatga tttcgcgcac tgctttacca aattcgcggc tttcccacgc
     1621 ttcaccaatc acttcagcgg catcagtgaa ggttttgtac aactgcgggt cagccagttc
     1681 gcttgccagc acgccgtcaa aacgcttgtt gataaagccc gcattacgag aggccaggtt
     1741 aaccactttg ttcacgatat cggcattcac acgctgaacg aaatcctcca ggttgagatc
     1801 gatatcatca atgcgcgaag agagtttcgc agtgtagtag taacgcaggc tgtcagcgtc
     1861 aaaatgattc agccaggtgc tggctttaat aaaggtgccg cgagacttgg acatctttgc
     1921 gccgttcacc gtcacatagc catgaacaaa caggttggtc ggcttgcgga agttgctgcc
     1981 ttccagcatg gcaggccaga acaggctgtg gaagtaaaca atatctttac cgatgaagtg
     2041 gtacagctcg gcggtggagt ctttcttcca gtattcatcg aagcttacgc tgtcgctgcg
     2101 cttgtcgcac agattcttga aagaacccat gtagccaatc ggtgcgtcca gccagacgta
     2161 gaaatatttg cccggcgcgt tcggaatttc aaaaccaaag tatggcgcgt cgcgggagat
     2221 atcccactgt tgcaggccgg attcaaacca ctcctgcatt ttatttgcca cctgctcctg
     2281 caacgcaccg ctgcgggtcc atgcctgcaa catttcgctg aaagagggca gatcaaagaa
     2341 gaagtgttca gaatcacgca ttaccggcgt agcgccagaa accaccgatt tcggctcgat
     2401 caattcagtc gggctgtagg tcgcgccgca gacttcgcag ttatcgccgt attgatccgg
     2461 ggatttacat ttcgggcagg tgcctttcac aaaacggtcc ggcaggaaca tgcctttttc
     2521 cggatcgtac agctgagaga tggtgcggtt tttaataaaa ccgttctctt tcaggcgaga
     2581 gtagataagt tctgacaact ggcggttctc ttcgctgtgc gtcgagtgat agttgtcata
     2641 gctgatgtta aagcctgcga aatcagtctg atgctcctga ctcatttcgc caatcatctg
     2701 ctccggggtg ataccaagct gctgagcttt cagcatgatc ggtgtaccgt gggcatcgtc
     2761 ggcgcagatg aagttgacct cgtggccgcg cattcgctgg taacggaccc agacatcagc
     2821 ctggatgtgc tccagcatat ggccgaggtg gattgagccg ttagcgtacg gcagtgcgca
     2881 cgtcaccaga attttcttcg cgacttgagt catagtaggc attacttctt aatagtgaaa
     2941 aggggttaga tagtaccaaa tgggaaaatg ttaagtaagt acttctctat attcttcagg
     3001 taaactatca gctgatggtt tatctataca aaaaaggagt cgggatgaac gaacaatccc
     3061 aggccaaatc accagaagcc ttgcgcgcaa tggtcgccgg gacactggcc aattttcagc
     3121 acccaaccct gaagcataac ctcaccacgc tgaacgcgtt gcaccatgtc gcctggatgg
     3181 acgacacgtt gcatgtggaa ctggttatgc cattcgtctg gcatagcgcg tttgaagagt
     3241 taaaagagca atgtagcgcc gaactgctgc gtatcaccgg ggcgaaagct atcgactgga
     3301 aactgtcgca caatatcgcc accttaaaac gcgtgaagaa tcagccgggt attaatggtg
     3361 tgaagaacat cattgcggtc agttccggta aaggcggcgt gggtaaatcc tccacggcgg
     3421 taaacctggc gctggcactg gcggcggaag gggcgaaagt cggcattctg gacgccgata
     3481 tctacggccc atcaattccc accatgctgg gcgcagaaaa tcagcgtcca acttcgccgg
     3541 acggtaccca tatggcaccg attatgtctc atggcctggc aaccaattca attggttatc
     3601 tggtcactga cgacaatgcg atggtgtggc gtggaccgat ggcgagcaag gcgttaatgc
     3661 agatgttgca ggaaaccttg tggccggatc tcgactatct ggtgcttgat atgccgccag
     3721 gcaccggtga cattcaattg acgctggcgc aaaacattcc agtaactggt gcagtcgtgg
     3781 tcaccacccc gcaggacatc gctttgatcg atgcgaaaaa aggcattgtg atgttcgaga
     3841 aagtcgaggt tccggtgctt ggtattgtcg agaacatgag tgtgcatatt tgcagtaact
     3901 gtggtcacca tgaaccgatt ttcggtaccg gtggtgcaga gaaactggct gaaaaatacc
     3961 acacacagct gctggggcag atgccgttac acatctcctt acgtgaggat ctcgataaag
     4021 gcacgccaac ggttatcagc cgtccggaga gcgaatttac cgctatctac cgccagctgg
     4081 ctgaccgcgt tgcagctcag ctctactggc agggtgaagt cattccaggc gagatttcct
     4141 tccgcgcggt ctaacgccta ttaaccattc tggttattct tcatacaccg cagagatgtt
     4201 acattgatgc ggtgtattag tttttgccgc attaatgaac acttatttaa tactgcgaat
     4261 attctgcgga ccttcgtccg aaactctaca aagtcgattg atcatacagg caatgcttca
     4321 ttatcatcct tcgtggctgt gtagtggatg tacagctata gtttgttatg cactcattct
     4381 atccaggcat aaccttcaat gaataagtat tggctgtcag gaattatttt tcttgcctat
     4441 ggattggctt ctcccgcatt ctcttcagag actgccacat tagcgattaa tggcaggatc
     4501 agtcccccaa catgcagtat ggctatggtt aatggtcaac ctcaaccgca ttgtggtcag
     4561 ttaacgtata atgttgacac ccgtcatctg ttttcttcgc ccgtcaaagg tgtaacgaca
     4621 gaagtggttg ttgcaggtag cgatagcaaa cgtcgaatcg tgttaaatcg ctacgattaa
     4681 tttggcaacc tgatttttat tgatatcaac aataccattt acataaatct acattaattg
     4741 atagatgcga taaatagtta atactcttaa atatttgttg tatttatatt gcaatggtta
     4801 atttgtttca aataaataaa acaatgtgtt tacattgcgg taactttttt gatgtgagaa
     4861 atttgtttcc aagagataca gactcttaac aaccaaaaaa aaattttatt aaggaacata
     4921 ttatgaaacg ttcaattatt gctgccgctg tcttttcttc tttttttatg agcgctggag
     4981 tatttgctgc agacgttgat accggaacat taactattaa ggggaatatt gcagaatctc
     5041 cgtgtaaatt cgaagcgggt ggtgattcag taagtattaa tatgccgact gtaccaacca
     5101 ctgtctttga aggtaaagct aaatattcta cctatgatga tgcagtcggt gtaaccagca
     5161 gcatgttaaa aattagctgc ccgaaagaag ttgctggtgt aaaactctcg ttgattacca
     5221 acgataaaat aaccggtaac gataaggcga tagccagtag caacgatacc gtgggttact
     5281 atctctattt aggtgataac agcgatgtcc tggatgtttc tgcacctttt aacattgaga
     5341 gttataaaac agcggaaggt caatatgcta ttccgtttaa agcaaaatac ctgaaactga
     5401 cagataactc agtgcaatca ggtgatgtgt tatcttctct ggttatgcgt gtggcgcagg
     5461 attaataaat aaaattgtaa gtttctccag gtaataaaca tggccgctat cccatggcgg
     5521 ccttttaatt taagaggcat taaaatgaaa ggattattat ctttactcat tttttctatg
     5581 gtccttcctg cacatgccgg aattgttatc tacgggacgc gcattattta cccggcagaa
     5641 aataaagaag tgatggtgca gttgatgaac cagggaaacc gttcttcgct gctgcaggcg
     5701 tggattgatg atggcgatac gtcattacca ccagaaaaaa ttcaggttcc tttcatgtta
     5761 acgccaccag tggcaaaaat aggggcaaat tccgggcagc aagtaaaaat caaaattatg
     5821 ccgaataaac tgcccactaa taaagaaagc attttttatc tgaatgttct ggatattcca
     5881 ccaaatagtc cagagcaaga aggtaagaat gcactgaagt ttgcgatgca aaacagaatt
     5941 aagttgtttt accgaccagc gggtattgct ccggtaaata aagcgacatt taaaaaattg
     6001 ctggtaaatc gcagtggcaa tggtttggtg ataaaaaatg actcagctaa ttgggtgacg
     6061 atttcggatg tcaaagctaa taatgtcaaa gtcaattatg aaactattat gattgccccc
     6121 ttagaaagtc agagtgttaa tgtcaaaagt aataatgcaa ataactggca tctgaccatt
     6181 atcgatgacc atggcaacta tattagtgac aaaatttaat tgcaggagct gcctatgttg
     6241 agaatgaccc cacttgcatc agcaatcgta gcgttattgc tcggcattga agcttatgca
     6301 gctgaagaaa cctttgatac ccattttatg ataggtggaa tgaaagacca gcaggttgca
     6361 aatattcgtc ttgatgataa tcaaccctta ccggggcagt atgacatcga tatttatgtc
     6421 aataagcaat ggcgcgggaa atatgagatt attgttaaag acaacccgca agaaacatgt
     6481 ttatcaagag aagttatcaa gcggttaggc attaatagcg ataacttcgc cagcggtaag
     6541 caatgtttaa catttgagca acttgttcag ggtgggagct atacctggga tatcggggtt
     6601 tttcgtctcg atttcagtgt cccgcaggcc tgggtggaag aactggaaag t
//
LOCUS       DAJTVJ010000188         6623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000188 DAJTVJ010000000
VERSION     DAJTVJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.189"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(25..606)
                     /locus_tag="OO448_003968"
     CDS             complement(25..606)
                     /locus_tag="OO448_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339050.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(653..4510)
                     /gene="sigA"
                     /locus_tag="OO448_003969"
     CDS             complement(653..4510)
                     /gene="sigA"
                     /locus_tag="OO448_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6339051.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4871..5875)
                     /locus_tag="OO448_003970"
                     /pseudo
     CDS             complement(4871..5875)
                     /locus_tag="OO448_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5860..6237
                     /locus_tag="OO448_003971"
                     /pseudo
     CDS             5860..6237
                     /locus_tag="OO448_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6276..>6623)
                     /locus_tag="OO448_003972"
     CDS             complement(6276..>6623)
                     /locus_tag="OO448_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6339052.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1644 a         1472 c         1494 g         2013 t
ORIGIN      
        1 cacctcaaaa taccgggaca gagatcaggc gtgattaatc tgcagcattt ttaataacag
       61 aagtactgag cttactttac ctttgatgat gtaatagtta tttccgtcgg tactcatttt
      121 tccctgaaat aatgtcacgg atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt
      181 catattttta atgagctgag atccgggtgg caaggggtat ttgctgtcag atactgggcg
      241 actgagtctg atgtggcgcg tatacagccc gttttcgtac agggtggcct ctgttatggt
      301 gtcagtgacc agcatgtggg agacaatgct gtattctttt ttaaagcact caccagaaac
      361 ggtgattgta atgataatgg tcttctcgat cgagatgtaa atacccgttt cgttatactg
      421 tccgtatcgg aaccatgaga actcagttgc attccagcca tgctccagaa tatgtgcatt
      481 tttttgcctg atagtttctt tttttattcg ctcatgttta aggcataaca ggcagataaa
      541 taatgtcaga aaaagaaagc taataatgag agcaagagcg atatagtctg tcacagggta
      601 atacatattc tctacggtag aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag
      661 agtaacggaa gttggcgttg atcgcgttat ccacgttgta tttaccaaat gccgatttct
      721 caaactcaag accgaagcga agattatcgc gaatttcggc gttgagacca acattcatga
      781 gaatacgacc gtctttttca cctttgatac gtttctcacc ggacgcatca cgcagtacgg
      841 tttcaccgtt ggcaaacagg tcaaactggt agccaaggcc ggcgcgggct gtgactttcc
      901 agtccttacc ggagaaggat ttacccacat caacaccggt acgcccaatc agcggattaa
      961 agtccttatc cttcatggtc acgctcattc cgcgatcctg ccagtcgaac cgtttacctg
     1021 atacggcccc ataaaccagt tctgcctgcg gctcaatcca ggagtcttct gttacatgat
     1081 agcggtagcc tgcttcagca cccacataca aggaatgaga actgtagtct tttgttccga
     1141 gtccagcaaa ggtcgccgag tactcattat catggtgaac atacttacta atcaggtcga
     1201 tataggcacc agagtcaaat atagcggaag catacagacc tgccccgacg gacttcgtct
     1261 tgccactgaa tgcattactg ctggcatgac tgtcggtata cgtcatagtc agaccagtga
     1321 aaaggtcaag tccatccagc tcatgttttt tatccacacc aatctgcaca tgggtgtagt
     1381 tgtcactgta tccactagaa gctgaacctg ctccgctcat gatgcgggcc catgcaccgg
     1441 cctcgccatt ggtgtcacgc agatccccca ttcgtttatt caggttgttg acttctgtca
     1501 ggaaggcttt gtaattaccg gacatatacg attttgctgc accggaagat gctgtatttt
     1561 cagccacctt atatccggtc agggtccacg tggtatgttc ctgatcctct ttcgtgctga
     1621 tcacaggaga tatgttgctg aaacctatgg cctgagtact ggctttaaat atctctttat
     1681 tgcttccttt tggggctgtt atcaggtcaa cgtttaatcc tgagttatta tttcccactt
     1741 tctgtatatc gaccgacaga ctgttattgc ttccggttaa ctgattttta acaatgagtt
     1801 tatccgattg atttacagac tggctcagat cagcccgcat aatcacagca gtattagtgg
     1861 catccagtgt gtttactgtc acagtatgga aacccgaatt ctctatgtta ctaaacaaca
     1921 aaagactgtc gcgggttttt aatgattgca gggctgactg tccgtttact ctccataagg
     1981 tgttatacat gctggctgag gcagcaggag catcaatacc tccggtataa gaagtatcaa
     2041 agccacccag caaacctgta gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt
     2101 tatactggtc aacaccaaac agaatagatg cttttttgtc tgattttata ttaccattga
     2161 cccaggcata attttgagcc tttaacgtgg caccatcttc tgtcatatta tatccatctg
     2221 acatatagat tgccgggctg tatttattat tttctgtagg ggtgcctgtt aatgtcaggg
     2281 ttccggccag ttcaatagct ttatctacat acatttttcc ctgggcggta acagtagctc
     2341 ctttgttgat aatcaggtct gtattaatta aggataatgc accagtcata gtaacagttg
     2401 atttatcacc aacagttatt ttgctgtttt gagcactcag actgccgttg aaactacttt
     2461 cgcctgctga atccccggag cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt
     2521 ttcttcctga gaacatgtca atgtatgcag ttttatttcc cagaacaatt tgagagtttt
     2581 tagcctctat gtgtcctttt acattagcat cccggcctat actcagggtt gcgttttcta
     2641 aatttaattt ccggaaatta aatttacgac tttcccagtc gggctgggaa aaagaggcta
     2701 tctgattgtt ggacttatat tccgtattgt aacgtttatt tacagtgctt tcctggtcct
     2761 ttatggcagc agaatagtct ttttgacata aaaaaggaat tggacagttg ttattgcctt
     2821 ctttaaatat ggcatgttct gtcgcatgtc cttgtaatgt taactgtgca ttccggacag
     2881 agatatcgtt tttgatatcg actgagccat caaagatcag attggcatta ttgttatgtt
     2941 gctgggtagt attgataata tgatttatgc ttatattacc tgatacattc ccatggtaca
     3001 tatatgcatc agtattagta agtgataatg ttgattgctt cacgtttgag ttcgttacgg
     3061 ttgttcccgc atctgttgct gcgattttct gaaatgactg gtcatagccg tttagatcca
     3121 gtgttccgcc atttctggta aaatagattt cgccattacc gctttcgctt aaagcgtctt
     3181 tggcatttat ttttaccgta cctttacctc cggccatgta aattttattg aaggcttttt
     3241 cagcactaag gatgacagtc ccattaccta ttttaaggtt atttccctgt gctattttta
     3301 catccagagt accactacct attttatgca gattatctcc ggcaacgccc ttaacattcc
     3361 attcaacggt ggtattatta tcaatatcta tcccggcacc tttataagac ttatcttttc
     3421 cgtacacttt atatgtctgg ttcttatcga atataagccc gccaatgcca agatcggtat
     3481 cctgcttgag cgttaattcc cctcctcctg agacagacag gtccttatct tttataatcg
     3541 atgataactc ttgtttttta ccatccagct gcatagtgtt tgtgaaattc ttttttaaat
     3601 tgtttaccag aatattatta aataacgagt agtttgtcca ggctatattg gtactactga
     3661 gaaaaccaac tgtactggta acaccatgga aaacccattt ttgttgttct ttatcatata
     3721 gataagaacc agaaccacta tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg
     3781 tataaatgta actcttgttt ttccagtcca gattaaaaag acttgcgctt aacaagtcgg
     3841 gattatagtt ttgtccaaac agatattgtt tgccgtttaa tatcgttgag gttgttccgg
     3901 cacctgctct gaagccaatt atttgttcct taccgttgta gtttacgcca tatcttttca
     3961 tcgcatcttc gggagaaagt gagaagtcgg cactctccgt aacccctgtt gtttccacga
     4021 cgaatttatt cagacgatga accgaaaagt cgccagatga tactctgtta cttgctttat
     4081 atgctgtctg atcccatgac tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg
     4141 cagtaacact gtacgcacct ccaattgatg ttgttgcacc tttgttggaa acagcagaaa
     4201 aatcaggtac aggtagttct ggaaaatgaa atatctttcc atctttcatc attaattgaa
     4261 catttgttgc acccggtctg aactctcctc tgttttgagc aaggtcaaga taatcgcggg
     4321 tccatatatt atttatatcc agcattgctg caaatgctgt ttcgccataa cttccatata
     4381 ttgaggataa aataataccg aggataactt tctttcttga tgtaccgaca ctaactttcc
     4441 gggtcagttc agaaacagca actaatccac ctgtaatatg actatatttc agtgaataaa
     4501 ttttattcat acgtgaactc cgatatttct gagttgtggt tatgggatag catttaatta
     4561 caacggggaa ctatcagtca tggtacgtaa ctgataaggg tataatgata atcactgtca
     4621 gttatatttg tatgacagat gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt
     4681 ggtcagatat tgatcacttg gtgtttgtat tttgaacttt gcgatcaaaa tttgatcttt
     4741 gttgtttccg gtcaatcctt tacaggtttt ctgtattatc cgggggagtt aagtctgtcg
     4801 atgtccgaca gacttttgat gatggaaaca acatgcagcg ataatgtgca tgctgttttg
     4861 caggtggaac tcagaaggtg aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc
     4921 cgatcaggcc tgcaacatca aggtgaacca catcaaacgg tgagatcccc accccggccg
     4981 tgaatgcact gccgtcgtta ccggccagat tctgacggta accggcgcgc agctgtgccc
     5041 atttccaggc attaaattct gcgccaatgg cggcaaactg acggttgctg tcggaggtga
     5101 aaccgctggc cggggtcaga tccacatcaa atgcggtggt gaacatcgcg ttgtgccagg
     5161 agacaccggc cgtcacctgc gggcggatcc tgaaggtttc cgtgataccg ttcacttctt
     5221 ttgtctcaat actacggggg atcaggttct gtgcgaccag tcccagcgtc cagttgtcat
     5281 ccaggtccgt actggcaccg atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt
     5341 cgcctttgaa ggcgctgctg tcataatttc tgaccagcac gttgtagttg aacaggtcga
     5401 cgcgctggaa tttggggggg aagcccagag accatttctg cccggccgtt tccagttccg
     5461 tagccagagc aatacccaca tcagtgatca gtgccgcgcg ggcaaaggcc tgtgatgtca
     5521 ggcggctttt gtccacctcc tgcccggtgc tgtttgccac ctcttccagg taagtgaggt
     5581 cagcatcaga gactttaccg tcgacactga cggtgccgtg tgatttaacc atcagtgcgg
     5641 cagagaggcg atcaccggga agggccgcca cagcagaaac tcccagctgt gcggcggcat
     5701 gtgtgttacg gaagtcccgg aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt
     5761 ggttactttc gatggcgcgg tcgaaggcct tccactcatc actgatccgg tcggcgttgt
     5821 cggtgatatt atccggattg gagagctgcg cgccgacgga cggaagcagg atgctgggct
     5881 catgcgcgac gcaaaatcca cgatgtatat atcagtacta aaagcgcgac ggcggaagaa
     5941 gctctgaaac taatcggtga actgtacgcc atagagcacg atatacgcgg gttaccggtg
     6001 tctgaacgcc tggcggtcag gcaaatgcag agtagaccgc tcctgacttc cctgtataag
     6061 ctgatgcagg agaaagaaca cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg
     6121 tatatcagga agcactgggc tgcgttgtgc aacttctgtg atgatggcct ggctgaggta
     6181 gataataatg cagcagaaag agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg
     6241 aggatcactc attccgtgtc tgactgcttc agggtttaat tttgcttctg ctgactgaat
     6301 gatgattggg cagcaagtgc aatatcccac ataaatccag ttaactcacg agccaccgcc
     6361 gtaatggcta cttgtgattt tttccctttt gctgtcagag ttcggtaacg agaacacaat
     6421 cgttgttgag ctttccatgc aatatctttg gaatattgtg ttacagagtc cggtcggtaa
     6481 tatataagcc atgctccaac ttttgcaggt gtacgataag accaagcggc ttcgtaaagc
     6541 agacgtctca gttcgctgtt tccaactttt gttattcctc tgggacgaat actgtttcca
     6601 ctggaatatt cacctggcac cag
//
LOCUS       DAJTVJ010000189         6537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000189 DAJTVJ010000000
VERSION     DAJTVJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.190"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="OO448_003973"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="OO448_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6339053.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="OO448_003974"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="OO448_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6339054.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="OO448_003975"
     CDS             2408..3157
                     /locus_tag="OO448_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6339055.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="OO448_003976"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="OO448_003976"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OO448_003977"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OO448_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6339056.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="OO448_003978"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="OO448_003978"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6339057.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="OO448_003979"
     CDS             6018..6491
                     /locus_tag="OO448_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6339058.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAJTVJ010000190         6532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000190 DAJTVJ010000000
VERSION     DAJTVJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.191"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..906)
                     /locus_tag="OO448_003980"
     CDS             complement(<1..906)
                     /locus_tag="OO448_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCR6339059.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="OO448_003981"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="OO448_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6339060.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="OO448_003982"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="OO448_003982"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6339061.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="OO448_003983"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="OO448_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6339062.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OO448_003984"
     CDS             complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OO448_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6339063.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6387..6532
                     /locus_tag="OO448_003985"
                     /pseudo
     CDS             6387..6532
                     /locus_tag="OO448_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1686 a         1686 c         1526 g         1634 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga ca
//
LOCUS       DAJTVJ010000191         6502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000191 DAJTVJ010000000
VERSION     DAJTVJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.192"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            28..240
                     /locus_tag="OO448_003986"
     CDS             28..240
                     /locus_tag="OO448_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339064.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            273..491
                     /locus_tag="OO448_003987"
     CDS             273..491
                     /locus_tag="OO448_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6339065.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            646..879
                     /locus_tag="OO448_003988"
     CDS             646..879
                     /locus_tag="OO448_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6339066.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(912..1550)
                     /gene="ompW"
                     /locus_tag="OO448_003989"
     CDS             complement(912..1550)
                     /gene="ompW"
                     /locus_tag="OO448_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6339067.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1790..1879
                     /locus_tag="OO448_003990"
                     /pseudo
     CDS             1790..1879
                     /locus_tag="OO448_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1907..2650
                     /locus_tag="OO448_003991"
     CDS             1907..2650
                     /locus_tag="OO448_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6339068.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2680..3219
                     /locus_tag="OO448_003992"
     CDS             2680..3219
                     /locus_tag="OO448_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6339069.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3324..3722
                     /gene="yciA"
                     /locus_tag="OO448_003993"
     CDS             3324..3722
                     /gene="yciA"
                     /locus_tag="OO448_003993"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6339070.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3762..4481)
                     /gene="tonB"
                     /locus_tag="OO448_003994"
     CDS             complement(3762..4481)
                     /gene="tonB"
                     /locus_tag="OO448_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6339071.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4572..6092
                     /locus_tag="OO448_003995"
     CDS             4572..6092
                     /locus_tag="OO448_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6339072.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6125..>6502)
                     /locus_tag="OO448_003996"
     CDS             complement(6125..>6502)
                     /locus_tag="OO448_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339073.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1706 a         1489 c         1549 g         1758 t
ORIGIN      
        1 atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat taacactgaa
       61 aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac tggcgacgag
      121 aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc aaattattgc
      181 tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca caacgattaa
      241 tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga aaagaatatc
      301 cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta ttgatgatac
      361 cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca gttactgaat
      421 tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt aatccgtata
      481 aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa cgaattacag
      541 gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct tagtccgtgg
      601 ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt taagccagca
      661 acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg gcgtgctaat
      721 ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt cagagagcag
      781 ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga tatttacaac
      841 gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt gcagaggttt
      901 tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg atccagtcgt
      961 accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc gatatccatg
     1021 taccacactg acatgttaac caaccagtca cggttaatca gataatcaac ccccacctgc
     1081 ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc tttgccatga
     1141 tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac ataaggacgg
     1201 aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg cagatgatga
     1261 acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg cgtcgctgcc
     1321 agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc cagttgcgtg
     1381 ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc ttctgttgga
     1441 cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc aaaggcactg
     1501 ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat atccgctccg
     1561 tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa tgatcctaca
     1621 tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt agaagttgca
     1681 ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct acgcaaatat
     1741 taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat tacctccgct
     1801 ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct taaccagggt
     1861 acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt ctatcacggc
     1921 gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga ccattctgtt
     1981 ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct cacccagtga
     2041 tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg ggttgttcga
     2101 cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt cagcggcgtc
     2161 cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat taattatcca
     2221 gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca gtgcgccgat
     2281 attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga ttggcatcat
     2341 gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac cggtgatgct
     2401 ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc tgacttgggc
     2461 gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa cactgttgct
     2521 gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac tggcgaacac
     2581 cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt atatgttgat
     2641 tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt tcttgatttt
     2701 ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc ggctactgcg
     2761 gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt tcgtaaggtt
     2821 gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt gacgctgttc
     2881 ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct gtttgcggga
     2941 gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat gctgggtaaa
     3001 gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc tgttttcttt
     3061 atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa tatttgggtc
     3121 aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt aagcggtatc
     3181 tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca gtccttcggg
     3241 gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg cgcctgtgat
     3301 tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag ggcgatcttg
     3361 ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc tttggtggtt
     3421 ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt gcccacggtc
     3481 gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg gtcggcgatg
     3541 tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc attaatattg
     3601 aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa gcgacagaag
     3661 cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta cctgttgagt
     3721 aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat ttcggtggtg
     3781 ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc cggctcataa
     3841 cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg cttggctgag
     3901 aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac tttaacctgc
     3961 ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg gcttaatgcg
     4021 cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc cgtacttgat
     4081 gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc tacgggtttg
     4141 acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg cttaggtttc
     4201 ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat cggctcaggt
     4261 tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg cggttcgaga
     4321 tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag ttcaataacc
     4381 tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca gaccgaaagt
     4441 aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta gctattcggc
     4501 attgtagcat tagccataac gtttataagt tcatctttaa atgataatca attgcatggg
     4561 agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc atacaaggcg
     4621 ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg ctgaagaaga
     4681 atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt tacaaaggta
     4741 aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg gagtttccag
     4801 aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag catcttgaaa
     4861 cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca ttgcaagatg
     4921 gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc atccagcgtg
     4981 tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt tctcagaaat
     5041 gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag atggttcctg
     5101 gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg agattgatat
     5161 tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt caaagcaacg
     5221 gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac tctgtacgca
     5281 ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt aatgaccgcc
     5341 ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat aaagctcaag
     5401 agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca ctaacgatta
     5461 acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct accggaacaa
     5521 agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc tgtgatgttc
     5581 caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg gggatgattg
     5641 acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat gaggttgaaa
     5701 ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat gatattgatg
     5761 aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag accacgattg
     5821 ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt aaagatattg
     5881 accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag catctaaaag
     5941 ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact gaaatcacca
     6001 tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt gctgataaag
     6061 agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag ccccttttta
     6121 tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga gcattacccg
     6181 gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc tcaccatttt
     6241 taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct gataactcac
     6301 ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg ctcgttgtag
     6361 tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg gtctttccag
     6421 tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct tggaaggtca
     6481 tctgaatacg aagaccgtta gc
//
LOCUS       DAJTVJ010000192         6497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000192 DAJTVJ010000000
VERSION     DAJTVJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.193"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1163)
                     /locus_tag="OO448_003997"
     CDS             complement(<1..1163)
                     /locus_tag="OO448_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6339074.1"
                     /translation="MLIYLYPVLLLFNILPVFFYGQMNSDLERFFGVPIGYIPDLIFY
                     FFVVLTSIITLRFHVSLWTKKLLFLGIIFLIYISIQMLLLSADISGVVILLSFFSNFI
                     ALVLLVSFCIGKDELYLTHSVRNINVVMCFGIICGVVKLFIGYSEDSNFIVYLNRNAT
                     AIIVVCFYCVYSYFYRGRKSWYVSSVLYSLFFLFLDSRAGIISFAISLFFVFLQLTKK
                     EKLLISLFFVPLLTLGISFTDIGTRLERMLSSSQVIFSGGNTLTKSQNDYRRVELVFI
                     GVDVLKENYLIGTGLGVANYVKAIDKKFLGSTNFGLAHNFYLSYSAQLGIIGFILLIS
                     VFYIMLSPIFKCGGYIGKGCVFALAFYVFFNEYILTPAIYIYISIFLSVVFIR"
     gene            complement(1232..2512)
                     /locus_tag="OO448_003998"
     CDS             complement(1232..2512)
                     /locus_tag="OO448_003998"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR6339075.1"
                     /translation="MFRKMIDAGGTFLLKAIFQIGVFVYFTHVSDITTFGIISYVFTV
                     YWFVLNFSDYGFRTKLVKDISDNSYSASELLSRSDGVKTYVFFFIFIIFMFYSYVSDS
                     ISLTLLVYISSAYFVCISSGRFSLLQAVGRFRCELYINIYSTIIYIGCNLFLSLFIEP
                     LYYSAISIFIYSISLLVFSSHKCNVPCFHIKRPSILVYKDFLDATPFAILVLLNVVLS
                     SIDLFILKEYFSYNSVAIYQVVTRVNTGLIIVFNVIYTVLLPSFSYYLKNSEWGNIRK
                     LQRYISLLVLLLCLCYYFFGIYFVGILFGDEYKVISSATFLIMFMALIKYNFWLINEL
                     YLVCSGNQSERVKSYCIGVVISMAVFFYFIPRYGWSGAVFGSAIATLVIGIFYIISVK
                     KDCGKILHDKYSLMMIFVPIFFYFIINGQQRLLY"
     gene            complement(2601..3626)
                     /gene="tviC"
                     /locus_tag="OO448_003999"
     CDS             complement(2601..3626)
                     /gene="tviC"
                     /locus_tag="OO448_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosaminuronic acid C-4 epimerase TviC"
                     /protein_id="HCR6339076.1"
                     /translation="MSRYEEITQQLIFSPKTWLITGVAGFIGSNLLEKLLKLNQVVIG
                     LDNFSTGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQAALGS
                     VPRSIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEENIGN
                     PLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWTAAML
                     KGDDVYINGDGETSRDFCYIDNVIQMNILSALAKDSAKDNIYNVAVGDRTTLNELSGY
                     IYDELNLIHHIDKLSIKYREFRSGDVRHSQADVTKAIDLLKYRPNIKIREGLRLSMPW
                     YVRFLKG"
     gene            complement(3719..4999)
                     /gene="tviB"
                     /locus_tag="OO448_004000"
     CDS             complement(3719..4999)
                     /gene="tviB"
                     /locus_tag="OO448_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HCR6339077.1"
                     /translation="MKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTIGFDINKSRIDE
                     LRNGHDSTLECSNLELLEATKLTYACSLDALKECNVFIVTVPTPIDKHKQPDLTPLIK
                     ASETLGKIIKKGDVIIYESTVYPGATEEDCIPVVEKVSGLKFNIDFFAGYSPERINPG
                     DKEHRVTNILKVTSGSTPDVAEYVDQLYKLIITVGTHKASSIKVAEAAKVIENTQRDV
                     NIALINELSIIFNKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQS
                     VGYHPEMILAGRRLNDSMGQYVVSQLVKKMLKQRIQVEGANVLVMGLTFKENCPDLRN
                     TKVIDIISELKEYNINIDIIDPWCSTDEAQHEYGLTLCEDPKVNHYDAIIIAVAHNEF
                     REMGESAIRALGKDEHVLFDLKYVLDKKSIDMRL"
     gene            complement(5068..6174)
                     /locus_tag="OO448_004001"
     CDS             complement(5068..6174)
                     /locus_tag="OO448_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6339078.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFHQAYARILNQPLDT
                     NALANGLFSDLILIAESPDTKVKFLESTEYYKKETNNLSTEQDKKIWLAEQANKGLVI
                     TPPKEKGNTSYYIIQASADSAQEAYKLLQGYLKNVNNQAVTLSLDEFGQNVNTLLVNL
                     NKEIIDIDFQRKSEKLDQIAHIQRDLTTAEQAGIIDYRSSKGGFDNAQSSYKFLLGEK
                     LLSAELKATKDAPIIYPFRYYEVKRQIDELEGMLRDNIQAQAYRYQMKPSEPVIKDKP
                     NKALILILGALPGAMFAIVGTLVYATLKDKTKLD"
BASE COUNT         2354 a         1256 c          912 g         1975 t
ORIGIN      
        1 ttacgtataa aaaccaccga taaaaaaata gaaatataaa tatatatcgc tggcgtcaat
       61 atatactcat taaaaaagac atagaaagcc aaagcaaaaa cgcatccttt accaatatac
      121 cctccgcatt taaaaattgg agacagcatt atataaaata cagaaataag caaaataaaa
      181 ccaataatcc ctaactgagc cgaataagat aaataaaaat tatgcgccaa cccaaagttg
      241 gtacttccta aaaacttttt atctatagcc tttacataat ttgcaacacc taatccagtg
      301 ccaattaaat aattttcttt taaaacatca accccaataa atactaactc aactcgacga
      361 taatcattct gactttttgt aagagtgtta ccaccagaga atataacctg tgacgaagac
      421 agcattcgtt caagacgagt gcctatatca gtaaaagaaa tacctaaagt tagaagagga
      481 acaaaaaaca atgatattaa taacttttcc ttctttgtta actgaagaaa aacaaaaaac
      541 aacgatatag caaatgatat tattcctgct cggctatcca gaaaaagaaa gaacagagag
      601 tacaatacag atgagacata ccaagacttt cgaccacgat aaaagtatga atatacacaa
      661 taaaagcaca ctactataat tgcggtggca tttctattta aataaactat aaaattacta
      721 tcttcagaat aaccaataaa taattttaca actccacaga taataccaaa acacattaca
      781 acatttatat ttctaaccga atgagttaaa taaagctcat ctttaccaat gcaaaatgac
      841 accaaaagaa ccaaagctat aaaattagaa aaaaacgata ataaaattac gacacctgat
      901 atatccgctg ataacaacaa catctgaatg ctgatataaa tcaggaatat gatgcctaaa
      961 aataataatt tctttgtcca cagagaaacg tgaaacctca acgttattat agatgttaaa
     1021 acaacaaaga aataaaatat tagatctgga atatagccaa taggaactcc aaaaaaacgc
     1081 tctaaatcag agttcatttg tccataaaaa aaaaccggaa ggatattaaa taacaaaagt
     1141 acaggatata ggtatattaa catacttaac ctttattagg cttccaatcg agtctaaaca
     1201 tattaatgga acgatataaa accacaacat attaatataa caaccgctgc tgaccattaa
     1261 taataaaata aaagaaaatt gggacaaaga tcatcattag tgaatactta tcatgaagaa
     1321 ttttcccaca atcttttttc acagaaataa tataaaatat tccaattact aatgttgcaa
     1381 tggcacttcc aaaaaccgcc ccactccatc cataccgagg tataaaataa aagaaaaccg
     1441 ccattgaaat gaccacacca atacaatacg atttaactcg ctcgctttga tttccactac
     1501 acacaagata aagttcattt attagccaaa aattatattt aataagagcc ataaacatta
     1561 tcaaaaatgt tgcagaagat attaccttat actcatcacc aaacaatatc cctacgaaat
     1621 agatgccaaa aaaataatag cataaacata gtaataagac taacagtgat atatatcgtt
     1681 gtaatttcct tatattaccc cattcagaat ttttcagata ataagaaaat gaaggcaata
     1741 aaacagtata aataacatta aacactatta ttagaccggt attaacccta gttaccacct
     1801 gatatatagc aacactatta taagagaaat attcttttaa tataaaaagg tcaatactag
     1861 ataaaacaac atttagtaac accagaatag cgaacggagt tgcatccaaa aaatctttat
     1921 aaactaaaat acttggtctt tttatatgaa aacatggcac attgcattta tgtgatgaga
     1981 aaaccaaaag cgaaattgag tatatgaata ttgatatcgc actataatat agaggttcga
     2041 taaacagaga taaaaataaa ttacacccaa tatatataat tgttgagtag atatttatat
     2101 ataattcaca tctaaaccga ccaacagcct gtagcaagct aaatctacca cttgaaatac
     2161 aaacaaaata tgcagatgaa atataaacaa gcagagttaa tgaaattgaa tcagaaacat
     2221 aagaataaaa catgaagatt ataaaaatga agaaaaaaac atatgtttta actccatcac
     2281 ttcttgataa taattctgat gcagaataac tattatcaga aatatctttc actaattttg
     2341 ttctaaatcc ataatcagag aagttaagca caaaccaata aacagtaaac acataactaa
     2401 taataccaaa tgtagtaata tctgacacat gtgtgaaata aacaaaaact cctatttgaa
     2461 atattgcttt aagtaaaaat gtaccacctg catcaatcat ttttctaaac atacactgac
     2521 catttagaat aaatacatgg attttcaccc ctgcattaat aacagaggtg aaatagatta
     2581 tttattcatg ttaatataat ttagcctttt aaaaatctca cataccacgg cattgaaagt
     2641 cgtaatccct ctctgatttt tatatttggt ctatacttta gtaaatctat agccttagta
     2701 acatcagcct gagaatgcct aacatctcca gatctaaact ctctatactt aatgctcaat
     2761 ttatcgatat ggtgaattaa attaagctca tcataaatgt aaccagataa ttcatttaac
     2821 gttgttctat caccaactgc aacattatat atattatctt tagcactgtc cttcgctaat
     2881 gcagataata tattcatttg tataacatta tctatataac aaaaatcacg actcgtttca
     2941 ccatcgccat taatatatac gtcatcacct ttaagcattg ctgctgtcca ttttggaatt
     3001 actgcagcat aagctccatt aggatcttga cgacgaccaa atacattaaa ataacgtaat
     3061 ccaatagttt taaaaccata tgttcgagca tatacctgag cataaatctc gttaacatat
     3121 ttagtaactg cataaggaga aagtggatta ccaatgtttt cctctacttt tggtagtgcg
     3181 ggatgatctc cataagttga gcttgatgca gcataagtaa aactttgtac ttgtgcattt
     3241 ttagccgcat gtaagatatt caaaaatcca gtaatattag ttgcattggt tgttatagga
     3301 tcaacaattg aacgaggtac agaacctagc gcagcctgat gtaagacatg atcaacacct
     3361 ttcataactt gctcacaggt agtgagatct cgaatatcac cttctataaa gcaaaatcga
     3421 ctccactgtt cagtggaaac taatgtttta acttcatcaa gattatattg atgtcccgtg
     3481 gaaaagttat ctaacccaat aacaacctgg tttaatttaa gtaacttttc taaaagattt
     3541 gatcctataa agccagcgac accagtaatt aaccaagttt tcggtgaaaa aattaactgc
     3601 tgtgtaatct cttcatagcg agacatataa atatccatgt atattaatca agtgctaaac
     3661 ctagcataaa atgctgaggt gtatcatatc aaagaggatt tgattttttt aatcactctt
     3721 acaagcgcat atcgatactt tttttatcaa gcacatattt taaatcgaac aaaacgtgct
     3781 cgtctttacc taatgcacga atagcgctct ctcccatctc gcgaaactca ttgtgtgcaa
     3841 cagcgataat tattgcatca taatgattaa ctttaggatc ttcacataaa gttaatccat
     3901 attcatgttg tgcctcatcg gtagaacacc atggatctat aatatctata ttgatattgt
     3961 attcttttaa ctctgaaata atatcaatca ctttagtgtt tcgtagatct gggcaattct
     4021 ctttaaatgt aagccccatc actaacacat tcgccccttc aacttgaatc cgttgtttca
     4081 acattttttt gactaactgg gaaacgacat actgccccat actatcattt aaacgacgtc
     4141 cggctaaaat catctccgga tgatagccga cactttgcgc tttatgtgta agataataag
     4201 gatctacacc tatacagtgg ccacctacta aaccgggcct aaaaggtaaa aaattccact
     4261 tcgtacctgc agcctcaaga acctctaagg tatcaatccc taacttatta aatataatag
     4321 ataactcatt aatcaatgca atattgacat ctcgctgcgt gttttcaatt acttttgcag
     4381 cctctgctac ttttatcgat gatgctttat gcgtaccgac agtaattatt aatttatata
     4441 gctgatctac atactcagca acatccggtg tagatccact ggtcacctta aggatattag
     4501 ttacacgatg ctctttatcc ccaggattaa tacgctcagg tgaataaccg gcaaaaaaat
     4561 caatattaaa cttaagacct gatactttct ctacaactgg tatacaatct tcttctgtcg
     4621 ctccagggta aactgttgac tcataaataa taacatcgcc tttctttatt atcttaccca
     4681 atgtttcaga tgctttaatt agaggtgtta gatctggctg tttatgttta tcaattggag
     4741 ttggaacagt tacaataaat acattacact cttttagtgc atctaatgaa caggcgtacg
     4801 tcaatttagt tgcttctaac aactctaaat ttgagcactc taatgtacta tcgtgaccat
     4861 ttcgtaattc atcaatacga gacttattaa tatcaaatcc aatcgtcgtt actttctttc
     4921 caaactcaac agcaagaggc aatccaacat aaccaaggcc tataatccca attttcgcat
     4981 tcaaagtatc gaatttcatt ttcttatcca tcataaagtg cacatagaac atctatttcg
     5041 cattgacaca acaatacgta acccagttta atctaacttg gttttatctt ttaatgtcgc
     5101 ataaactaat gtaccaacta tagcaaacat tgcccctggt aatgcaccaa gaatcaaaat
     5161 taatgctttg ttgggtttgt cttttataac tggctcagat ggcttcattt gatatcgata
     5221 tgcttgcgcc tgaatgttat cgcgtaacat tccttctaac tcatcaattt gacgtttcac
     5281 ttcgtaatat ctaaatgggt aaataattgg cgcatcttta gttgctttta gctctgctga
     5341 taacagtttt tcgccgagca agaacttata gctactttgc gcattatcga agccgccttt
     5401 gctagagcga taatcaatga ttccggcttg ttccgcagtt gttaaatctc gctgaatatg
     5461 agctatttga tcaagctttt ctgattttct ctggaaatct atgtcaataa tttctttatt
     5521 tagattaacc aaaagagtat taacattttg accaaactca tcaagactta atgttacagc
     5581 ttgattatta acattcttta gatatccctg cagtagttta tatgcctctt gcgctgagtc
     5641 tgccgatgct tgtattatgt agtaacttgt atttcccttt tcctttggtg gcgtaatcac
     5701 aagaccttta ttcgcttgct cagctaacca aattttctta tcttgttcag tagataaatt
     5761 atttgtttcc tttttataat actcagtact ctctagaaat ttaactttgg tgtctggcga
     5821 ttcagcaatc aaaatcaaat cggaaaacaa tccattagcc aacgcattcg tatctaacgg
     5881 ttgatttaat attcgggcat acgcttggtg aaatttaaga tagctgccta attcagcaat
     5941 acgtggtgct tgaatatagg ttttagccgt ccactgctct tttgcggtaa aggcatacac
     6001 tgctgctaac cccgtagtca gcaacgtgac acataaaata acccattttt tcttccacaa
     6061 ggtgcctaaa agctcgaaaa gatcgatttc atcctctgct tttggcatat aagttggata
     6121 gaccccttgc gggatcagat aaggggtctg ttgtggttca gatgcttttg acatgaaacc
     6181 aatccattat ttatcaatgg gttaaaaata aaaatgaaga ataagcctga caaaaaagta
     6241 cgctaccgcc cttgagtttc agctcccaaa gcgctgcgca gaaacaggat atagccaact
     6301 gcgatacaac aagtgatatc tgggattaga ggacataatc gagaacactc cgtgtgaaga
     6361 gacatataca gtcccacact tccctgctgc aaaccggcgg atagcccgca gaatttggag
     6421 tgcagattat aaagcaatgc ctgtataaga ccaaatctgc cttcaaaatg ataacagtat
     6481 gtttgtaaat tctgtga
//
LOCUS       DAJTVJ010000193         6397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000193 DAJTVJ010000000
VERSION     DAJTVJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.194"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(112..462)
                     /locus_tag="OO448_004002"
     CDS             complement(112..462)
                     /locus_tag="OO448_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6339079.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(505..969)
                     /locus_tag="OO448_004003"
     CDS             complement(505..969)
                     /locus_tag="OO448_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6339080.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(966..1463)
                     /gene="rrrD"
                     /locus_tag="OO448_004004"
     CDS             complement(966..1463)
                     /gene="rrrD"
                     /locus_tag="OO448_004004"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCR6339081.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(1463..1678)
                     /locus_tag="OO448_004005"
     CDS             complement(1463..1678)
                     /locus_tag="OO448_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6339082.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2359..2433)
                     /locus_tag="OO448_004006"
     tRNA            complement(2359..2433)
                     /locus_tag="OO448_004006"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2398..2400),aa:Gly,seq:tcc)
     gene            complement(2437..2512)
                     /locus_tag="OO448_004007"
     tRNA            complement(2437..2512)
                     /locus_tag="OO448_004007"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2477..2479),aa:Thr,seq:tgt)
     gene            complement(2515..2591)
                     /locus_tag="OO448_004008"
     tRNA            complement(2515..2591)
                     /locus_tag="OO448_004008"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2555..2557),aa:Arg,seq:tct)
     gene            complement(2593..2669)
                     /locus_tag="OO448_004009"
     tRNA            complement(2593..2669)
                     /locus_tag="OO448_004009"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2633..2635),aa:Ile,seq:cat)
     gene            complement(2859..3422)
                     /locus_tag="OO448_004010"
     CDS             complement(2859..3422)
                     /locus_tag="OO448_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6339083.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(3526..3744)
                     /locus_tag="OO448_004011"
     CDS             complement(3526..3744)
                     /locus_tag="OO448_004011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339084.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(3903..4289)
                     /locus_tag="OO448_004012"
     CDS             complement(3903..4289)
                     /locus_tag="OO448_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6339085.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(4279..4650)
                     /locus_tag="OO448_004013"
     CDS             complement(4279..4650)
                     /locus_tag="OO448_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6339086.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(4650..4907)
                     /locus_tag="OO448_004014"
     CDS             complement(4650..4907)
                     /locus_tag="OO448_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339087.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(4904..6295)
                     /locus_tag="OO448_004015"
     CDS             complement(4904..6295)
                     /locus_tag="OO448_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6339088.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(6292..>6397)
                     /locus_tag="OO448_004016"
     CDS             complement(6292..>6397)
                     /locus_tag="OO448_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6339089.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT         1536 a         1753 c         1458 g         1650 t
ORIGIN      
        1 cctggttaaa tttcattttt cgcgggtata aaaaaacgat ggggcgggca gtccggaaga
       61 cgtcaggtca cagggatttg ccccgcccct ccctacaagt gagaataatt atcaccggat
      121 tcgttcgcgc gctgttttcg ctttatggca gggccagcac agactctgca ggttgctgtc
      181 tgcgtctgtt ccgccatgcg ctttcgggat gatgtggtcg acggttttcg cctcgctcac
      241 cacaccgaca cgcagacaca actgacacag acctttatcg cgcttcagaa tacgggcacg
      301 aatcaccgtc cattttgagc catagccacg ctggtggcgg ctcagtccgc gctggtgctg
      361 cacccagcct tcaccgcgat gtttatcgca gtaacccgaa ctgtctgtgg ttgtacctgc
      421 acatccacgc ttacggcagg cacgtgggat tagtgatggc ataaatacct cataccctgc
      481 gaaatgttta ccacgataaa aaggctactt aatgcactga gtgcggatat actcctgtgc
      541 cccttccagt tgcatctgca tcgtcatcag ccgctctctg agggtgaaat aatcccgttc
      601 agcggtgtct gccagtcggg ggctggttgc attatccacg ccggaggcgg tggtggcttc
      661 acgcactgac tgacagactg ctttgatgtg caaccgacga cgaccagcgg caacatcagc
      721 gcgcagagtt tcattttcag ctttcgcatc agctaactcc ttcgtgtatt tagcatcgag
      781 tgcattaaca tcacgctgtc tctgctgcat gtcagtaatg gttgcgttcg ccagcttcag
      841 ttctcttgcg tttttgtcgc gctgctcttt gtaggtaatg gcgttatcac ggtaatgatt
      901 aacagcccat gacaggcaga cgatgatgca gataaccaga gcggagataa tcgcggtgac
      961 tctgctcatt gctgccccca caaacagact tcacgctcaa tctcacgacg ggtcatcagc
     1021 cctttccatt gcttaccgcc agcatatgtc cagcgacgta gctggtcaca tgcgcctttg
     1081 atatcgccct ggtttatttt gcgaagaagc gtcgatgttc tgaaattgcc agcgcccacg
     1141 ttgtaaacga acgagtaaag agcgccgcgc gttgtttccg gtatatcgac tttgatgtac
     1201 gggttaattt gtctggcgac agtggcaagg tctttattca ggagggcttt gcattctgct
     1261 tcggtatacg ttttaccgag catgatgtct tttccggtgt gtccgtgaca tacagtccat
     1321 acaccaatga tatctttgta tggtatgtag ctgacacctt ccagaccatc gttaccacct
     1381 gggccagtga ttaacacaga tgctatagca acagccccac caccaatagc agctgcaaca
     1441 gccttgcgta atgatggcga cattattcac ctctcgcagc cttacgcttg tcttctctta
     1501 ttttgaaata cagatttgtc agataagtca gaagccccag aagcagactt cccagcacac
     1561 caatcgcagc ccactgtgat ggactgacct gatccaacca ctgcaaaaac cagtagccgg
     1621 cactgccggc ggaggtgccg taggcaatgc ctgttgatat tttttccatc tgatacatat
     1681 cccgcccccc gacagacctg tgctatcgga aagaaaaaag gccatcagca aaactctgat
     1741 ggcctgaatc acctttacca atattgtatg aaaaaacacg cacgactaat tgacaataat
     1801 tttcatttcc attaaaaaag atcgcgtagc attcttaatt catgaggaac tttacccgcc
     1861 agcaatctga gtagcgaaag ctgttcatcc ccaatgtttt tgctggcggg tccttttttc
     1921 tacggtcctc ctccccggag gggggcattt cattattttt ctaatcgtta ctggtgagaa
     1981 ggcggaagaa aaagccagtt cttcgcccct aaataacagc atgattccag tccatgcaaa
     2041 atctgatttc cagctcacac ataccgagcg ctgtacaaaa aaccgccagc caaggcagtt
     2101 aagagtgtgt tcccggggtt tgcttagaat attttgtaag ttgtccgaag agatatttac
     2161 aacaccagaa tgatgaatca tcagtcccct gccagaggtt cactacgtaa cctgaccgac
     2221 aatgcaccat tctggtgctg aaaaaagagc actggaactg caacaaaaca acatcacaga
     2281 acaggagatg gtaaggagtc agacattccc acacaatatt gtgtcagtgc aaataacaac
     2341 cttcgtctca aatctcgctg gagcgggcag cgggaatcga acccgcatca tcagcttgga
     2401 aggctgaggt aatagccatt atacgatgcc cgcatatggt gccgactacc ggaatcgaac
     2461 tggtgaccta ctgattacaa gtcagttgct ctgcctgctg agctaagtcg gcgctggccc
     2521 accaccgagg actcgaacct cgcaccgtca acttagaagg ttgatgctct atccggatga
     2581 gctagtggtg gttggtggcc cttgctggac ttgaaccagc gacctggcga ttatgagtcg
     2641 ctcgctctga ccaactgagc taaagggccg gaggcagaat aataaccata tgtcatcaca
     2701 tctgcaaact catctgacca ccagcacgtt taacgtcctg tgccgttttt caggtataaa
     2761 aaaacccgct catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat
     2821 tcaacttttt acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc
     2881 accagttgct ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt
     2941 tccagagtac ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt
     3001 ttatcgaagt ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct
     3061 ggtggctgtt tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga
     3121 tcggtttcga gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag
     3181 cgaacgttac gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt
     3241 aacgcatcac cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga
     3301 ttgaaattgt ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt
     3361 gtcatgacag gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc
     3421 atagacatgt cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca
     3481 aaaccgtcga aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg
     3541 gtcataaacc aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc
     3601 tttcgaggtg ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac
     3661 cgtcaggttc caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac
     3721 cggcgagctt ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg
     3781 acaataaaaa acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc
     3841 cgacgccaga ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc
     3901 acctacataa ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac
     3961 atgccatcaa taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac
     4021 ccatgtttcc gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa
     4081 tcgtgcaaat cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca
     4141 tcatcatcag aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca
     4201 gcagcgatcg acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt
     4261 tccataacct gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac
     4321 gcccagtcgc ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc
     4381 gtcgtctatg agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc
     4441 caggtcacga cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga
     4501 caggtttaat ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc
     4561 accaactttt gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg
     4621 cggataaggt aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca
     4681 aactttccaa ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt
     4741 tgtgcgcgat gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg
     4801 gcatcttttg ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg
     4861 tgtttacgca attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca
     4921 tcttactgct gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg
     4981 tggaaagaac ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc
     5041 ggtaaatcgt ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg
     5101 tagcctgtgt atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat
     5161 ccgcctctat ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg
     5221 actccacacc gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca
     5281 ggcctttaag cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg
     5341 tttttatcag cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat
     5401 ggtgtttcgt tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga
     5461 tattgcgccc ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa
     5521 agtgagccac agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt
     5581 caccaatctg gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag
     5641 tcaccttgtc gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa
     5701 tgaataccag gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg
     5761 tcatcaacgt cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt
     5821 ccggaagcac atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga
     5881 ccatcatgtt ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac
     5941 gtaatttctg cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc
     6001 gcaacgctgc aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag
     6061 taatcaccgg ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg
     6121 aaacacgggc ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa
     6181 tgtccgccgg tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga
     6241 atgcgccaat gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc
     6301 cacacctctg atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc
     6361 gtctgtgatc ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAJTVJ010000194         6385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000194 DAJTVJ010000000
VERSION     DAJTVJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.195"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..326
                     /locus_tag="OO448_004017"
     CDS             6..326
                     /locus_tag="OO448_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCR6339090.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="OO448_004018"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="OO448_004018"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6339091.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="OO448_004019"
     CDS             2213..2929
                     /locus_tag="OO448_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6339092.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3382..3457)
                     /locus_tag="OO448_004020"
     tRNA            complement(3382..3457)
                     /locus_tag="OO448_004020"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3422..3424),aa:Asn,seq:gtt)
     gene            complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="OO448_004021"
     CDS             complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="OO448_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR6339093.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5206..5281
                     /locus_tag="OO448_004022"
     tRNA            5206..5281
                     /locus_tag="OO448_004022"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5239..5241,aa:Asn,seq:gtt)
     gene            complement(5319..6269)
                     /gene="cbl"
                     /locus_tag="OO448_004023"
     CDS             complement(5319..6269)
                     /gene="cbl"
                     /locus_tag="OO448_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6339094.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1814 a         1542 c         1373 g         1656 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
     3001 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
     3061 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
     3121 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     3181 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     3241 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     3301 tttttacaag gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag
     3361 agatgtctca aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat
     3421 taacagtccg ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt
     3481 agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat
     3541 aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggttcg
     3601 gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc
     3661 cgcacagccc agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg
     3721 atgcccagca cataaccgac gacaacccgg caaccccaca tgctcaacat agaaacccac
     3781 atggcgtaac gggcatcgcg agcaccttta aatccagcgg gtagcaccca tgaggcggac
     3841 caaataggca taaataaagc atttagccaa atcagaatca caacgacatg tttaacctgt
     3901 ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg
     3961 gccgttaatc caagagtgga aagccagaac acatgccgca actgaatctc tgcttgcgct
     4021 atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagagcc gagcgcactt
     4081 ccgggtaagt tgataagagc cgcaattgaa aacgcgataa aatttccggc aataacactg
     4141 gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat
     4201 tcgacactcg cgggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt
     4261 ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac
     4321 aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccagccctgc cccgacaaat
     4381 cccagtcccg gccaggagaa aaggccgtaa atcaatatgc cgctaataat aatattaaga
     4441 atattcaggc taccgttaat caatagcggt attttcgtat tccctgcacc acgaagtgcc
     4501 ccgctaccaa taagagtgat ggcagctgct ggataactga gtaccgtcag ctccagataa
     4561 gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt
     4621 tcgccaaaat gatgaataag cgttgccaac agtacggcaa acaacgtcat gatcaccaat
     4681 gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct taccgagact aaatgccaca
     4741 acgacagtag taccaagatc gatagcagca aaaaaagcca taatgaccat attgaagctg
     4801 tccgccaatc ccacgccggc catcgcatct tttcccagcc agctgaccag aaaagtgctc
     4861 agaaccccca tcaacaggac acaggcattc tccatgaaga taggaacagc aagcggggtt
     4921 atctcgcgcc agaacaacac tttgtagctc ttgcgtttag cgtgccagcg agtgccgtga
     4981 acaacctggc gtaaagcaga ggagatattc aaagccgacc ttaattgcag aaagtgaaac
     5041 cacatttcaa ataatgaggg agaatcagca aagctgcaaa gattttcgcc aacaaattgt
     5101 ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt tggggaattt aaaaatcggg
     5161 tttgacaaaa gatttttcgc cgttaagatg tgcctcaaca gcgattcctc tgtagttcag
     5221 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     5281 aaatttctga aaagcccgct tttatagcgg gctttttgct atatctgata atcaatttcc
     5341 tcttcactgt tttccatcac ctgccgcttg atatcctcaa ctgacagtcc tgcattacaa
     5401 agttccagaa agcgccagac atagttacgc tgaagttgtc ctcgcttcag tcccaaccag
     5461 acagtattag catcgaaaag atgccgcgta tccagacgga ttaaattctc ttcctcttgt
     5521 tcgccgctgg attgttcggc aactaatccg atcccaagcc caagagcaac ataggtttta
     5581 atgacatcag aatcctgcgc acttaataca atatctgcca gcaaaccttt gcgggcaaat
     5641 gcgtcatcaa tacgtgagcg ccccgtaatc ccctgtcggt aagtgattaa cggccacttc
     5701 gctattgatt ccagcgtcaa tggtgtaatt tgcgttaagg gatgatcgag tggaacaagc
     5761 aaactatggt gccaacgaaa ccacgggaag gcgacgagct gcgggtcatt actcaaacgc
     5821 tcgctggcga taccaatatc agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggc
     5881 gtcccctgga ttagctcgag ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc
     5941 tctggcaagc tataacgtgc ctgagtatgc gtcgttgcaa tagtgagaac gccagacgta
     6001 tcgttggtaa acaggtctgc aagccgacga acattactgg cttcattcag aatacgttct
     6061 gcaatgacca gtaatgcttt gcccggttca gtcatgccca gcagtcgctt acctcgtcga
     6121 acaaatattt cgatgccaag ttcatcctcc agttcccgaa tatgacggct gacgccggac
     6181 tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg
     6241 cggattatct ttagttgttg gaaattcacg gtaaactccg ggcagttcag atttcccgtt
     6301 attgttaaag tctaatgccc ggcataacaa ataataaaaa cccgcatctt attccatccc
     6361 gatataacgc ttagctcacc aattg
//
LOCUS       DAJTVJ010000195         6253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000195 DAJTVJ010000000
VERSION     DAJTVJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.196"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            122..952
                     /locus_tag="OO448_004024"
     CDS             122..952
                     /locus_tag="OO448_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCR6339095.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OO448_004025"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OO448_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6339096.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="OO448_004026"
     CDS             complement(2081..3541)
                     /locus_tag="OO448_004026"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6339097.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OO448_004027"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OO448_004027"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6339098.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6253
                     /gene="gntP"
                     /locus_tag="OO448_004028"
     CDS             5146..>6253
                     /gene="gntP"
                     /locus_tag="OO448_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6339099.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIIS"
BASE COUNT         1480 a         1618 c         1587 g         1568 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtg
//
LOCUS       DAJTVJ010000196         6221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000196 DAJTVJ010000000
VERSION     DAJTVJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.197"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            430..957
                     /locus_tag="OO448_004029"
     CDS             430..957
                     /locus_tag="OO448_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6339100.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWDDNGITRYITEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     TPEGEDYGFSDDIPF"
     gene            1015..1248
                     /locus_tag="OO448_004030"
     CDS             1015..1248
                     /locus_tag="OO448_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCR6339101.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            1307..3265
                     /locus_tag="OO448_004031"
     CDS             1307..3265
                     /locus_tag="OO448_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6339102.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAGSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRALAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALESDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVKDNTKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPVQISLECKHYSLTSDAPSGKEGAAFLVLMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3320..3754
                     /gene="psiB"
                     /locus_tag="OO448_004032"
     CDS             3320..3754
                     /gene="psiB"
                     /locus_tag="OO448_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCR6339103.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3751..4470
                     /locus_tag="OO448_004033"
     CDS             3751..4470
                     /locus_tag="OO448_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCR6339104.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            4467..5063
                     /locus_tag="OO448_004034"
     CDS             4467..5063
                     /locus_tag="OO448_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339105.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            5526..6026
                     /locus_tag="OO448_004035"
     CDS             5526..6026
                     /locus_tag="OO448_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR6339106.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLATFDDERDFFAACRSL
                     HQDEADPELMFQDYEGFPGNMASECHINWAYVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         1293 a         1684 c         1930 g         1314 t
ORIGIN      
        1 ggcttgcagc gaccgttccg gtcgcgccgc ctctgtcgcg ccgtgacctg cgctccctgc
       61 acacctgacg gtgtgtgagg aaactgcgcc tgatggcgca gcagtcttcc ctcctgccgc
      121 cacctctgcg gcctgtttcc cgctgtggac tttccggggg gcagacgtaa ccccctcccg
      181 ttccatctgt ccagggtgag atgcaccggc cacaaaattt ttagccatga acccggcaaa
      241 aaattttgcg tccgcccccg gacagctgtc ccgtgaggcg gtaaacgggc ccccatcgga
      301 aaggtccact gcggggagaa acccctgtga agtggacggc aggaggagct ttcgctcctc
      361 tgaaggggca catgaacgtc ccgtctccgg tatccggttt atctcacatc tttcagtcag
      421 gaggttctta tgagtgcacg tggtatcaac aaggtcatcc tcgtcgggcg tctgggcaat
      481 gatccggagg tccgttacat ccccaacggt ggcgcagtgg caaacctgca ggtggccacg
      541 tcagaaagct ggcgcgacaa acagacgggg gagatgcggg agcagacgga atggcaccgt
      601 gttgtgctgt tcggcaagct cgcggaagtg gcaggtgaat atctgcgcaa gggcgcgcag
      661 gtctacatcg aggggcaact ccgtacccgt agctgggacg acaacggcat cacccgctac
      721 atcactgaaa ttcttgttaa gaccacgggc accatgcaga tgctggggag tgcaccacag
      781 cagaacgctc aggcgcaacc gaagcctcag cagaatgggc agccacagag tgctgacgcg
      841 acgaaaaaag gtggcgcgaa aacgaaaggc cgtggacgta aggccgcgca gcccgagcct
      901 cagccgcaaa cgccggaggg ggaggattac gggttttcag acgacatccc gttctgatcg
      961 ggctgactgt gacaaccgcc ccgccctgtg cggggcatca ccggagatat gaggatgagc
     1021 gaatatttca gaatacttca gggactgccg gacggctcct ttacccgcga acaggcggaa
     1081 gccgttgccg cacagtaccg gaacgtcttt atcgaggatg accacggaga acattttcgc
     1141 ctggttgtcc gtaataacgg aacaatggtc tggcgtacct ggaattttga ggacggtgcc
     1201 gggtactgga tgaaccatgc catccgtgat ttcgggattc ttaagtaaga gaggtgccgg
     1261 acgcgcgaac gtccggcagg acataagcaa ttataagggg atgattatgc ctgtaacgaa
     1321 gtgtgaacca gaaaccaccc gcaaagcaag ccgtaaatct gcaaaaacgc aggaaactgc
     1381 cctgtctgcc ctgctggcgc agacggagga agtgagcgtg ccgctggcct cactgattaa
     1441 atcaccgctg aatgtgcgca cggtgccgta ttctgtggag tccgtcagcg aactggctgg
     1501 gtccattaag ggcgttggcc tgttgcagaa tctggtcgtg cataccctgc ctggtgaccg
     1561 ttacggtgtc gccgcaggtg gtcgccgact ggctgcactc aacatgctgg cagagcgcaa
     1621 catccttccg gctgactggc ctgtccgtgt gaaggtcatc ccgcaggaac tggcgactgc
     1681 cgcatcgatg accgagaacg gtcatcgtcg ggatatgcac cctgccgaac agattgccgg
     1741 attccgcgca ttggcgcagg aaggcaaaac acctgcacag actggcgacc tgctgggcta
     1801 ttcaccccgc cacgttcagc gaatgctgaa actggcagac cttgcgcctg tcatcctcga
     1861 tgcgctggca gaggaccgca tcaccaccga gcactgtcag gcgctggcgc tggagagcga
     1921 caccgcgcgt caggtgcagg tgtttgaagc ggcctgccag tcaggatggg gcggtaaacc
     1981 ggatgtgcgg gttatccgca acctgattac ggaaagtgaa gtggcagtga aggacaacac
     2041 taaattccgc tttgtggggg ctgatgcctt ctcgccagac gaattgcgca ccgatttgtt
     2101 cagcgatgac ggggacggtt atgtcgaccg cgtggcgctc gatgccgccc tgctggaaaa
     2161 actccaggct gttgcagagt tccttcggga agccgaaggc tgggaatggt gcgccggacg
     2221 tatggagcct gtcggtgagt gccgtgagga tgccggaaca taccgctgtc tgccggagcc
     2281 ggaagcggtg ctgacggagg cggaagacga acgcctgaac gaactgatga cgcgttacga
     2341 cgcgctggaa aaccagtgtg aggaatccga cctgctggaa gcagaaatga agctgatgcg
     2401 ctgcatggcg aaggtcagag cgtggacgcc ggagatgcgt gccggaagcg gtgtggtggt
     2461 gtcctggcgt tacggtaatg tgtgtgtcca gcgtggtgtg cagttgcgca gtgaggatga
     2521 tgcggctgac gacgctgacc gcacggaaca ggtgcaggaa aaagcgtcag tggaggaaat
     2581 cagcctgccg ttgctgacga aaatgtcttc cgaacgcacg ctggcagtcc aggcggcact
     2641 gatgcagcag ccggacaaat ctctggcact gttggcatgg acgctctgcc tgaatgtgtt
     2701 tggcagcgga gcgtacagta aaccagtaca aatcagtctg gaatgtaaac attattcgct
     2761 gaccagcgat gcgccgtcag ggaaggaagg tgccgcattc ctggtgctga tggcagaaaa
     2821 agcccgtctt gcggccctgt taccggaggg atggtcacgg gacatgacga cgttcctgtc
     2881 actcagtcag gaggtgctgt tatccctgct cagtttctgc accgcatgta gccttaacgg
     2941 tgtccagacc cgcgagtgtg gtcacacgtc acgcagtccg ctggattcgc tggagagcgc
     3001 catcggtttt cacatgcgcg actggtggca gccgacgaaa gcaaacttct tcggacacct
     3061 gaaaaagccg cagattatcg cagccctgaa tgatgccgga ctgtccggtg ccgcacggga
     3121 cgcggagaag atgaagaaag gtgatgcggc tgaacatgca gagcaccata tgaaagacaa
     3181 ccgctgggtt ccaggctgga tgtgtgcacc acatccacag acggatacca ctgaacgcac
     3241 cgataacctg gctgatgccg cctgatgaac aaccacaccg ccccgccgga gacggggcgg
     3301 cagcaaggga gataccgtga tgaaaactga actgaccctg aatgccctgc agtccatgaa
     3361 cgcacaggaa tatgaagata tccgtgctgc gggaagcgat atgcgccgta atctcactca
     3421 cgaggtgatg cgtgaagtgg acgcaccggc taactggatg atgaatggcg agtatggcag
     3481 tgagttcggg ggctttttcc ccgtccaggt tcgtttcacg ccagcccacg aacgtttcca
     3541 cctggcatta tgttcgccgg gagacgtctc tcagctctgg atgctggttc tggtgaattg
     3601 tggtggacag cctttcgccg tcgttcaggt gcaacatatc ttcacgcctg tcgctatcag
     3661 tcacacgctg gcgcttgccg cgacacttga tgcgcagggg tacagtgtta acgacatcat
     3721 ccatatcctg atggcagaag gaggtcaggc atgagcgcac gttcacgggc actgatcccc
     3781 ctcagcgcag agcaacaggc cgccatgcag gctgtggctg tcaccgaaca acgtcgtcgt
     3841 cagggacgca cactttcagc atggccttat gccagcgctt tctttcgctg cctgaatggc
     3901 agtcgccgga tttcgctgac cgatctccgc ttttttgctc ctgcgctgac gaaggaggaa
     3961 tttcatggca accgtctcct gtggctggct gccgtggata aactgattga aagttttggt
     4021 gaagtctgtg ttcttcccct gccatccgat gcggggcatc gtctgttccc gtccgttcct
     4081 tttcgtgaag gtgagcggcg tcgtcagaaa accacgctga cagagcagaa atacagccgc
     4141 cagcgggaac gtgaggcaga acgacgggaa ctggaatacc agacatgttt tgctcaggcg
     4201 cagattgacc ttgcgtttca tactcccgcc acggtcggaa gctggttgtc ccgctggtct
     4261 ggtgttgttg aggagcatga tctggaaacg attttctggg ggtggtgcgg gcgttttcca
     4321 tcactgtcat catttgaccg gtttttctgg caggaggaac cactctggcg gctgattttt
     4381 gaagccggtg aggccggtcg tggtgcaccg gtacaggtac gtgcacttga gcagtggatg
     4441 atcccgaaca agctggagaa cgcaatatga tgaaatcaga cgaacaatat caggttcccg
     4501 tgtggatgcg acctctgttg ccgttgctct gcaataccgg tggtaatgat ccggaggaac
     4561 tgctgaatga tacagaaacc accgccagtg cgaatattgt ccgttatgta ctgatagttg
     4621 cagtgcggtc gcagattgat ctgctgcagc tactgtacag gaaaggactg ttgcgcacag
     4681 agataccagg tggcttttca ccggaagaag cgcaggaact cctggataat ctggtgcgca
     4741 gccatatcag caaggcgttg tcgggtgagc gaatggcagc ccgtgacaga aatgccgatc
     4801 tgacctggat tcgccagcaa ctggtcgatg ccgcctggtt tgtccgtgcc acactggaag
     4861 cgcatgggat gggcgtcgga aatgagagtc cctctgctcc gccggagaca atgccggaca
     4921 tacagacacg ggaactggtt atgttgatca agcgactggc atcatcgctg aaagcggtga
     4981 aacccgacag tagcgtggtg cgtgaagcgc aggactggct gtgcgacaga aaacttgtgg
     5041 atatcacaga tattctccgg tgaaccagac atgtatattt tacgcaggga cacgccgatc
     5101 atgatcgttt catatcaggg gtggtctgga ggtcatgcgg cgtgtcctct gcactcgccg
     5161 gaataaggaa gtcgccggcg gctccgcttt tacccggcca tgcggggcat ggccttgtgg
     5221 gttttcagct ctgtggcctc agcgtcgtgt gcgggctgtg ccgtgcctcc atcttagccg
     5281 ggctggcagg gatgcaaggg tacgcttcgc cgctgcggtc acccggtccc tccttcccgt
     5341 ctgccgtgat tttccggtcg ttcacccgct cagatttcgc ggtctcaccc tccaactccc
     5401 gccagccgtg tgcgtcaggc tgcggcttcc cttgcgccct gtgcatcccc gccttatcgc
     5461 ccggctttta tggaggcacg gcaccgcccg gtgtcgcaga acatgtgact atggaggatt
     5521 cgggaatgtc tgttgttgca cctgctgtat acgttggaac ctggcacaaa tacaactgtg
     5581 gaagcatcgc tggccgctgg tttgacctgg ccacgtttga tgatgaacgc gactttttcg
     5641 ccgcctgccg ctctcttcac caggatgaag ccgatcctga actgatgttt caggattatg
     5701 agggattccc ggggaatatg gcctcagaat gccatatcaa ctgggcctat gttgaaggct
     5761 tccgccaggc gcgggatgaa ggctgcgaag aggcttatcg tctctgggtg gatgataccg
     5821 gtgagacgga ttttgacacc ttccgcgatg cctggtgggg cgaggctgac agtgaggagg
     5881 cttttgcggt tgagttcgcc agtgataccg gcctgctggc tgacgtgccg gagacggtgg
     5941 cgctctattt tgactatgag gcgtatgcgc gggatttatt cctggactcc ttcaccttta
     6001 ttgacggtca tgtgttccgt cggtgattta ctcccccgct ccggcggggg tatggcctgc
     6061 caggccgccg gaaaaccctg gcaggcttac gcctgcctga aatgcaggag ggccgcgcct
     6121 gcggcgctgc ccttcctgcc cgtcatggtg tgaggcattt ttctgttatg tcgtttctgt
     6181 tgttctgatt tccggaggcg gggacacctt cggctcccgg c
//
LOCUS       DAJTVJ010000197         6215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000197 DAJTVJ010000000
VERSION     DAJTVJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.198"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            245..367
                     /locus_tag="OO448_004036"
                     /pseudo
     CDS             245..367
                     /locus_tag="OO448_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(629..1195)
                     /gene="ipgB2"
                     /locus_tag="OO448_004037"
     CDS             complement(629..1195)
                     /gene="ipgB2"
                     /locus_tag="OO448_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6339107.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSREIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1478..1792)
                     /locus_tag="OO448_004038"
     CDS             complement(1478..1792)
                     /locus_tag="OO448_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339108.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(1870..1980)
                     /locus_tag="OO448_004039"
                     /pseudo
     CDS             complement(1870..1980)
                     /locus_tag="OO448_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2304..2981)
                     /gene="ospD1"
                     /locus_tag="OO448_004040"
     CDS             complement(2304..2981)
                     /gene="ospD1"
                     /locus_tag="OO448_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6339109.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKSNIINVTNSAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3352..3489)
                     /locus_tag="OO448_004041"
                     /pseudo
     CDS             complement(3352..3489)
                     /locus_tag="OO448_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3568..3774)
                     /locus_tag="OO448_004042"
     CDS             complement(3568..3774)
                     /locus_tag="OO448_004042"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6339110.1"
                     /translation="MDAENETVLNANMTHHLGCEKNQLRSGSNSRNGCLTKIITTGDE
                     PLEIRTLRDRNGTFEPQQLKKNQP"
     gene            complement(4458..4617)
                     /locus_tag="OO448_004043"
                     /pseudo
     CDS             complement(4458..4617)
                     /locus_tag="OO448_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4621..5775)
                     /locus_tag="OO448_004044"
                     /pseudo
     CDS             complement(4621..5775)
                     /locus_tag="OO448_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5795..6142)
                     /gene="tnpB"
                     /locus_tag="OO448_004045"
     CDS             complement(5795..6142)
                     /gene="tnpB"
                     /locus_tag="OO448_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6339111.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6142..>6215)
                     /locus_tag="OO448_004046"
     CDS             complement(6142..>6215)
                     /locus_tag="OO448_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6339112.1"
                     /translation="RLKGKLTPALLQILIREIKGSSH"
BASE COUNT         1599 a         1408 c         1322 g         1886 t
ORIGIN      
        1 aaattcaggc gaatgctgtt tacgtgtttt tttgctggtt gatactgttt ttgtcatgtg
       61 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
      121 caatttgctc actggtattt actacgtgca tcgaatgagt gatcaagagc cgtatgacgc
      181 gagagtgtca cgtacggttc cgtgagcagc tggagagtga aactctctcc ggctgactcg
      241 actcgcaggt tccgacagcg gtgacgaaca tgcagcggtg ttgttctcgc tgatcggcac
      301 acgccgtctg aacaatgtgg agccagaaaa atggctgcgt tacgttattg aacatatcca
      361 ggactgaccg gcaaaccggg tacgcgatct gttgccttgg aaagttgatc tgacttctca
      421 gtaaatatca atatgattct gacgagccgc ttacctttga gcagagaaag gggagaggga
      481 tcccacatcc atgagctagg aaaatacaaa aactggctat acaacaattc aatatattag
      541 gacttacttg cgaggagaaa tatagcaccg ctcaggcaat tcctgggtat ttacttttct
      601 tagtttcttt actcgggatg ttctgtcatc agaaaggcga ttctaaattt gtaatatagt
      661 cactcatatt cccacgtaat ttctttagat caatatattg cacatttttc tcaaaaaact
      721 gatcggaaat aagatttgca acattgcttg ttgtatcatt tttaacgctt ctattcatcc
      781 cttcacaaag ttcgtccatt ttctttttga aataactgct tcctgataca agatgattta
      841 tggaactttg tgcagcattg atgtctagag aaatgcgata atctttctca acctgtttaa
      901 atatctgttt acggaggtta tcgccgattt ctcggctgtg ttctgcacaa aacttatcaa
      961 tacattggtt taccacccgg gatacgtaga cttttttttc atcatttttc caatctgtaa
     1021 tattttcacc gatttgttct gaaacagagc tcaaaatact agtatttatt ttggttgatg
     1081 atatttttac tatccttttc cccattgtac agcggataat ctcatccacc tttcctgaaa
     1141 aatatctttt gtgagataaa ctgattccaa aattattaaa agatgttcca agcattataa
     1201 tcactttgtc atgtaaaaat agtaacattg tcattatttt agctgattca aacaattctg
     1261 taaatgcttt gagttcttta ctcccttggc tcaatgatgg accgtctgga atcccagaga
     1321 ctaaactctc tgagaaagag agtatgaaaa aacttggggc agtccctaaa tgaagggacc
     1381 aacagcggta aaataaccgg catcattcat aatgatgccc tgttagttgt agttatgaag
     1441 atttacgtca ttaaaattgc tgttcgtagt gtcagtccta tggtctggca tcagctgagg
     1501 atcgtagccg atacatcact ggcttcacga cattcgtgtt gaggctattc atgaaaactc
     1561 cacgctgaaa acgccgtttt gtataaacgg tcacggtatg cccggagcca ctgttgctga
     1621 tgaattcgat aaaaccttgg cgtttcttga agctatcgtt aatgcggata atgaaacaac
     1681 gattggtgag attcgctctt ttgccgacgc cctggacgct gtccggttca atcgcaacaa
     1741 aatcaaccgg cagctgagca aacccaacct tgcctctctg gcgctggagc atgaagtgat
     1801 ttggctggga cgtagccgct gacagcttct ataaaagtat gatcctaccc acgtaatatg
     1861 aacacaggct taagtgaggt tctggttttc aaattgttca ggactgagac ctccacacca
     1921 actgtgccgt cgtcagcgat tgtaatcgca cttgatataa ttaaacactg tatatttcat
     1981 ttgtggagtc tccagtctga gggggcttta tgcattccgt attgggcact atgatgccag
     2041 aaatctgcga ggctccacac cttcctgtat ttcccttcct ctatcacagt gcactttact
     2101 taatagggtg ggatgtgttc attacattta ttgtgttttt tatgattgtt tttgcgtcca
     2161 gtgtcgttcg tcatgggaaa ctggttctat gggtagtatc gctcagtcta gttaactgtt
     2221 tgtttctttt tagaaattaa gcagatcaca caattcgggg agagatcctt ctaagtgcaa
     2281 ttctttgcag ctatttagca cacttaaaac aactcttgta ttttgtcaga aataatatta
     2341 aacatgtctt gttcattatt gttaagtctg tctgctattt ctgtaagttt tatcccacta
     2401 tgatggcata tatttatata tgccatcata gcctcgatat tccctttccc tgcggccaga
     2461 aataatcctg gtaaaccctc attatctctt acatccaata gtttaatcgt ttcttttggt
     2521 gataaatcaa aagttttaat aaaatctcca taagcttgaa tcgcagcgga atgaccttct
     2581 tgaagagcta tgaacaaagc agattcccca gcattatttc gtgctgcaag aacttctagt
     2641 ttttcctctt tgggtatttc ggctgccaac agcgattcac cgtatttacg cgctgcatcg
     2701 gcatttccat gccaaatcaa tgaaaacaaa ttatggcggt tatactcctt attattcttt
     2761 gtcggaatgg atatgctctg tattttggcc aattttctga aagaaacacc actatatccc
     2821 ccccctgatt tttcttcatt gatggcgtgg gatatagcgg aatttgtcac gttaatgata
     2881 ttacttttca ttcctttatt ttcattagca gtattgctgc ctattattag gtgcatattt
     2941 ttgttgtttg ctggatgtaa tccatagtta tttattgaca tttttgttca tcctttttgc
     3001 taaaaaaaac aataacctat ttttgtaatg taatagatat tagtctattg ccaatctaaa
     3061 aaaaccattg ccacattgaa tggctatatt caccaataaa ttctttcgat ttcgtatatt
     3121 gacatatcgt agatatatct tatccctaag attcaataat cgagtccgtt taccattctg
     3181 tgcaagcgcc ttcactcaga agtgaccgtc aaggcgatca ccaaacttga taataaagca
     3241 gctcattgcc atacgccagt ccttaagggg cgttgtccat ttctgtgatg ctgcctagat
     3301 ttaactgcca gctccggctt atctacatct aaatatgtga gcctaatacc gctacacttt
     3361 ggccagacta tgtttttctc cgggaagtgg gctaaccgtc tccataaagt gacgaacttt
     3421 tttcaacagt ggccatattg aacggcactg atgattacgg gttattgtct catgaagagc
     3481 cggccacatc actgacagca gtgacttcag gccttcagca tcagacagcc tgaaccagtt
     3541 aatccatcgc ccgacagatt tagtaatcta gggctggttt ttcttcagct gttgcggttc
     3601 gaaagtgcca ttacgatcac gcagagtacg gatttccaga ggttcatctc cagtagtgat
     3661 aatcttcgtt aagcaaccat tgcgggaatt gctgccagat ctaagctgat ttttctcgca
     3721 cccaagatga tgagtcatat tagcattgag tacggtttca ttttcagcat ccaatcaaac
     3781 tgactgagat cttcaggatt tttgagactt ttggctagtt cattagctaa agcccgcaac
     3841 ttattagatg tcaactgttt ttcattcata aattcacctt catttgatat caaattaaat
     3901 agttcaacat cagagaatta tacaaactta tgtacaggct cctatgatct tcgaatcctg
     3961 aagattatag tgaaaataag ttttcacgtt gaaatcccac atagttagta tatgataaat
     4021 aatcatggat gacctgatta acctcttggg gtagtgcttt tatagaagaa taaatattct
     4081 actaatttaa tacatcacct aaaatagtat tgttttttat ggttggttga aggtcgtggt
     4141 gatgatgtta gtccttttga gaatattcca gtgccagatc ttacttataa taaaggggct
     4201 tggcagtttg gataaactat tgttcttatt ccactaatac aaactttttg taaagtatat
     4261 taaatatact tcatttcata ctgaaatccc cggaagtatt caatcaaaca attacaccat
     4321 aaaaggaaga gggggcccta attaagttgt caagcatgtt tatgatacct gcagggtata
     4381 aattttctgg ctgcaagcag acacatataa aacgtcttta ttgatcctac ccacataata
     4441 tggacacagt cgtaagtgag gctctggttt tcaaattgtt ccggactgag accaccacac
     4501 caacggtgcc gtcgccagcg attgtaatcg cactcgatat aactaaacac tgttgcccgc
     4561 actatttccc gatggataat agtgttctcc gtggagacat tccattttca gcgaatgaat
     4621 tttcatctgt tgctgtatcc agtcatacag tgactgcatc agtggcgcgg ctctggcttt
     4681 tcttgccgcc agacgctgtt ctgctgaaca tccccggact tctgcctcga tggcatacag
     4741 ttcaccgata cgctgctggg cttccgttat tctgccggat tcgtacaacg cccggtaact
     4801 accgtaagca tcggcctgaa gcactccgct gtaaccggcc agatgattct gtggatggat
     4861 acctttccgg tccggtgagt acgcgaacca gaccgccgga ggcatttccg aaccggcgtt
     4921 acggtcatca cgaacgtaga cccacagccg ggcagtcctt gttttaccgc tgcccggctc
     4981 ctggaccgga acggggatat catcggcatg gactttaccg ggcatcagca catactggcg
     5041 caggatgtca tacagcggtt ccagcagttc agcaacagca cctgtccagc gccccagtgt
     5101 ggcacggctc agctccacgc cctgacgacg gtatatttct gactggcggt ataacggcag
     5161 atagtctgca tattttccag cgacaacatg ggccagaagc cccgctccgg cataactgcg
     5221 tgcaatgggt tttgaaggca ccggtgcctg cacgatatgg tcacaccggc aacaggccag
     5281 tttcggacgt tgtgtttcga taaccttaaa ggcgctgctg ataagctcca gctgctctaa
     5341 cacatcacat cccagagaac tgagttcgcc accacaggcc gggcagcatt cctcttccgg
     5401 acggatgacc cgggtttcac ggggaagtga ggcaggtaac ggtttatggg ctgaagactg
     5461 gcgcagggga gatggcagta ccgggtcata ttgctcaccc agcgtttccg ccatttcttc
     5521 ctgaagtgcg ctgattcgct cctgtgcttc ctgtatctgc cgttcggttt ttgcgcgaag
     5581 tttttctgag cttttaccga actgcatgcg ctgcagtttc gcaaccagcg ccttcagccg
     5641 gttgatttcg gaagcataag ccgccacccg ctgtgagagc aggcggttgt atttcgccat
     5701 ctggcggatg gtgtcctgtt gcgtctgcaa cagtgcccgc aggcgggcat tctcatgagc
     5761 aagtgaggtg tccatatcct cactttacaa cgggttatat gcggatgcca gcgcgttccg
     5821 ttcgtttcgg gtgtttccag tcgatgccct caagaagcat ggataactgg gccggagtaa
     5881 ggtgcacctt gccgtcacgg gtgaccggcc agacgaagcg gccccgctcc aggcgtctgg
     5941 taaagaggca cagtccgtca ctgtcagccc acaacacttt tatctggtca ccccggcgtc
     6001 cgcggaagat gaacagatgc ccggagaacg ggtcatcctt caggacgttc tgaacttttg
     6061 aggccaggcc gttaaagcca tttcgcatat cggtgatacc tgcaaccagc cagatacgcg
     6121 aacctgcagg gaaagatatc atcagtggct gctccctttt atttcgcgga taagtatctg
     6181 taataacgcc ggagtcagtt tacctttaag cctga
//
LOCUS       DAJTVJ010000198         6201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000198 DAJTVJ010000000
VERSION     DAJTVJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.199"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            10..752
                     /locus_tag="OO448_004047"
                     /pseudo
     CDS             10..752
                     /locus_tag="OO448_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(794..1246)
                     /locus_tag="OO448_004048"
                     /pseudo
     CDS             complement(794..1246)
                     /locus_tag="OO448_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1310..1456
                     /locus_tag="OO448_004049"
                     /pseudo
     CDS             1310..1456
                     /locus_tag="OO448_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1456..2613)
                     /gene="intS"
                     /locus_tag="OO448_004050"
     CDS             complement(1456..2613)
                     /gene="intS"
                     /locus_tag="OO448_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6339113.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2775..2849)
                     /locus_tag="OO448_004051"
     tRNA            complement(2775..2849)
                     /locus_tag="OO448_004051"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2814..2816),aa:Arg,seq:cct)
     gene            complement(2925..3857)
                     /locus_tag="OO448_004052"
     CDS             complement(2925..3857)
                     /locus_tag="OO448_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6339114.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4151..4906
                     /gene="mlaA"
                     /locus_tag="OO448_004053"
     CDS             4151..4906
                     /gene="mlaA"
                     /locus_tag="OO448_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6339115.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5088..5423)
                     /locus_tag="OO448_004054"
     CDS             complement(5088..5423)
                     /locus_tag="OO448_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339116.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5381..5773)
                     /locus_tag="OO448_004055"
     CDS             complement(5381..5773)
                     /locus_tag="OO448_004055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339117.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5780..6156)
                     /locus_tag="OO448_004056"
                     /pseudo
     CDS             complement(5780..6156)
                     /locus_tag="OO448_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1546 a         1369 c         1372 g         1914 t
ORIGIN      
        1 gtggatgata tggcaactac atgttcagtt atattgattt tggagtcctt tgatgtttat
       61 ttcggaaaag agagtgtgtt tctggagaga ggttcatctg tacttgtcga ctctagctct
      121 agagattttt tcctgacata tcctgaaaga gtgatagtgg cggattttgg cgctgagttt
      181 attagtcgct atttgaaagc taataactta agggatattt ctgattgtag ggaatatcta
      241 tcttatttaa aaataaactt tgctgacttc agtttaatta aaggattaat tagttgggct
      301 aatcactgtg ctgaatacat agaaattttt gatgagtcta ttgcttttac atgtctctct
      361 gcattttctt ctgaaaaaca atttggagta tttctgtttg gatgtttgaa aagcacaggg
      421 ctaaagttaa aacgattatt catacggatt tatctgcacc atggcgtctt aaggatatat
      481 catcaagatt atatctcagc gaaagtttac taaagatgaa attgaaagaa gagggggtat
      541 cattcagtaa gatcatactt gatgagagga tgcaaatggc tgaatattta ctcagcactc
      601 gttgttatcc tattagtaaa gtagctaagg tctgtggtta tgccagtgtc tcatacttta
      661 cttatgtatt tagacgttat tttggtgttt ctccaagtca atactctcag aggagttcag
      721 aaagtaaaat tcttactcac cagggaatct gatcattgtt cttgccccct tatttccaga
      781 cagggggtgt atcttaagtt aacgttaccc gctgacgtcg atattctcgc ggagagcgat
      841 aacccagcgc actgtgcgga tggttttcat tgtaatgttc gatcgccact gcaagattat
      901 gcaatgccgt tcttacattc ggtttcggca tgaacgcgat gtagtcttcc ttcatcgttt
      961 tcacgaacct ttctgccatt ccattactct gaggactgct gattgccgtt gtgcagggct
     1021 ccagattcaa ctctctggcg aactgccgtg tttcatgcgc tctatacgct gaaccgttat
     1081 ctgtcagcca ctggatggac tgttccggta ctttatcgcc aaagcgtttt tctatcgctc
     1141 ctaacatgac atcctgcact gtcgctttat cgtaacctcc cgtgcttgcg gcccagtcta
     1201 tggcttcacg atcgcaacag tccagggcga acgtaacacg cagttttgaa tcgccacggg
     1261 tttaacagac acctcagagt catttaagat ggcttaaaga gaggtgccca tgagcggtaa
     1321 gcgttatccc gaagagttta aaactgaagc agtcaaacag gttgttgatc gcggttattc
     1381 tgttgccagc gttgcaacac gtctcgatat cactacccac agcctttatg cctggataaa
     1441 gaagtacggt ccggattact ccactttttc atcaagccaa tccgcccacc actgcatcat
     1501 ttctctgcgc ttatcgagat actgagcatg gttgtaaata ccgcgcacag atccgccgtt
     1561 ggcatgtgcc agctgcactt caatagcgtc agcaggccat tcgtgctcgt tcataatcgt
     1621 gctgaattca tgcctgaatc cgtgaccgct ttccagacct tcatagccga tttgtttgat
     1681 cacaagcaat accgcgttct cgcagattgg cttcttctta tcgttgcgcc cggcaaaaac
     1741 aaactctgag actggtttgg tgattgagct tagcgtagtg agaagttcaa ccacctggtc
     1801 tgacatcggg accacatgaa ttttgcgtcc cttcatcaca ctggcgtcga tggtgataat
     1861 cctgttttca aaatcgacgt tcttccatag catggaacga agctcttttg ttcttagggc
     1921 tgtgtagcgt aaaactttgg tcgcaatgag cgatacgata cttcctgaaa atgttgccag
     1981 tgctttgttg aatgcaggca tctggtctgc tggaaggaac gggaagttct tcttgcggta
     2041 tcctttcatg gcgtctgcaa ggtcaggtgc cgggttatat ttagcccttc cggtgacaat
     2101 agcgtaacga aaaacctcgc cgcatcttct gcgggctttg ttggctcgct ccattgcacc
     2161 gcgatcttca aatctgcgga ttacttccag cagttgcatc ggctcaatat cctgaatctc
     2221 aagaccgccg atgatgggta aaatgtcgtc atcaaacatt ttggcaagtt cagttgcata
     2281 gcctactgac cagacttgct tcttgtgctc gtaccattcc ttgtaaatcg cactaaatga
     2341 attgttgtta gacgaagcct ttttcgcctt taccggaacg atgccaaccg agatgtcttt
     2401 cctcgcagtc catgccttat cccttgcttc ctgcaaagtc ataagcggat attttccgac
     2461 ggtcaggatt ttctccttac cgtcaatctt gtagcgaagc tgccatacct ttttcccgga
     2521 tacaggaaca taaaggtaca ggccattacc atcgagaagg cggtatggtt tttctttcgg
     2581 ctttgctgct tcaatctgct taacggtgag catgggtaaa aatccggtgg gtaaaattat
     2641 tttatccact ttttacccgt catggagtgc ggctgtcaac gatctgacgc gaaccatgac
     2701 gaaccgtgaa tatacggaag gcttgatatt caggggattt tgcggactgg tacggatggg
     2761 agcgaactga taaatggtgt cccctgcagg aatcgaacct gcaattagcc cttaggaggg
     2821 gctcgttata tccatttaac taagaggaca atgcggcatg agtatacccg ctaatggagt
     2881 gcggggtaag tacgctgccg ctcgattgct taaaccctcg ccatttatgc cgggttttta
     2941 tcatttttct taatgttttc cgcacgttct gctttttggc gtgcttctgc tttacgcttg
     3001 ttgctcatgt cgttacgaat ctgtgcatga ctcattaacg cgaagataaa ggtgccgccg
     3061 cagatgttcc ccgctaaagt aggtagtgcg aagggccaga tgaaatcgct ccagtgcagc
     3121 gtaccgttaa acaccagata gaggatttca acagaaccga ccacgatatg ggtggtgtca
     3181 cctagggcaa taagccaggt catcaatata atcaccacaa tctttgccgc acctgctgca
     3241 ggaaacatcc ataccatagt ggcgatcagc cagccggaaa tgatcgcgtt ggcaaacatc
     3301 tcgctggggg tgttcttcat cacatccatg ccgattttga caaatgcatc gcgagtttct
     3361 tcattgaaga taggcatata ttcaaatgcc cacgcagcaa taccagtccc gagaatatta
     3421 cccagcagca cgactcccca taaccgcata agtaagccaa cgttgctcat tgtcggtttt
     3481 tgcatgacgg gtagtaccgc agtcacggtg ttttcggtaa ataattgctg gcgggccatt
     3541 atgacgataa taaaaccaaa ggtataaccg agattctcca gtaagaagct gcccggcaca
     3601 ccttccagtt cgacatgaaa tatccctttt gccagtaacg aagcgcccat cgacagaccc
     3661 gccgcaatgg ctgaccacag tagcgccatt gcgtcgcgtt ccagctcttt ttcaccatcc
     3721 tggcggatat gctcatgaat tgccatcgcc cgggagggga gtcggtcttc atctatttct
     3781 atttttttgc cgcgctcttt ttcttcgctc tcaacttcaa tttcgtcgct gtgttgatca
     3841 attttgtcgt tgtccatggt ctcttctctt gaattagcac gtatagctaa agcgtagcgg
     3901 cttttttgct cgcaactggc gggagttact ctgaaaatgt agaaaaggct gcgtttgcct
     3961 ttttctgttt ctatagaatc aagtagccta cagggcggcg attaccaggc tatgatcaaa
     4021 tcagcaaatc agggcgtctg gacatcagtt gacgtgctgt tacaatcgcc cacacctaaa
     4081 caggcggata cggtatcgtt ccgtcatgga tggcaaactg cataagccat aaaaaaacag
     4141 ggagacattt atgaagcttc gcctgtcggc gcttgctctg ggaactacgc ttctggtggg
     4201 gtgtgcgagt tccggtacag atcagcaagg gcgttctgac ccgttagaag ggttcaaccg
     4261 caccatgtac aacttcaact tcaacgtatt agacccgtat attgttcgac cggtcgctgt
     4321 cgcctggcgt gattatgttc cgcaaccggc gcgtaatggt ttgagcaact ttactggcaa
     4381 ccttgaagaa cctgcggtga tggttaacta cttcttgcag ggcgaccctt atcaggggat
     4441 ggtccacttt acccgctttt tcctgaacac cattttgggg atgggcggct ttattgatgt
     4501 tgcagggatg gcgaacccga aactgcaacg gactgaacct caccgcttcg gtagtacgct
     4561 tggtcattat ggcgtgggtt atgggcctta cgttcagtta ccgttctacg gtagcttcac
     4621 gctgcgtgat gacggtggtg atatggcgga tggtctttac ccggttcttt cctggctgac
     4681 ctggccgatg tctgtgggta aatggacgct tgaagggatc gaaacccgcg ctcagctgct
     4741 ggattccgat ggtctgctgc gtcagtcgtc cgatccttat attatggtgc gcgaagcgta
     4801 cttccagcgt catgatttca tcgctaatgg cggcgaactc aaaccgcagg aaaatccgaa
     4861 cgcacaagcg attcaggatg atttaaaaga tattgattct gaataagaaa caaataaaaa
     4921 aggtgagtcg caaaactcac cttttttgtt gttatccctc aattaccaca agtaaaagga
     4981 gataaaccga caatgacccg gacgtaatga tgtaaaaata ctgatgttcg tcactgacta
     5041 tttccgatat tcaggcgttg aaaaatcaaa aaactggaaa aataatttta aatatcatca
     5101 aaattaacac ctggcgcaga tagttgatta atttcatttt gatatttcgc ctggcgattt
     5161 tgaagtcttt tttcaacgtt atcttcgaga tccttaaagt ctttgtattt attttcagga
     5221 tggtcaacgt caactttgtt agcgaataaa ttagaaatta tttcttttac attgtcgcgt
     5281 ctaaattttg catcgtccac ttgaaaatta tatcttattg ctcgacctaa acgtttaaaa
     5341 acttcgttag gttctctcag tctttccccg ttgagctcgt ttacaacctg atgatcggaa
     5401 cctgttgcaa atgattctgc catgatagct ttactgcttt caaaccacgc tgaagcattg
     5461 ctgaagcatt cctggctttt tcactacaat attgggtgag actatcacca ttaatacgaa
     5521 ttcctctttg aatgtggtag tcgtcctgat cgtagtaacc gagagataag taatcatttc
     5581 cgtttttatt tttaattaca atagcgagag agttgccttt tttatcttta ccgtaccagg
     5641 taaataagtc cgttggttcg ctattcccgc tctcatcaaa ttctgttacc gtatgtctga
     5701 gtttgtgtga tatcttatcg aagataaaac caggatctcc atcatatatt tcttttaatg
     5761 cgctgaagat catttccttg ttattgatat catgagtctt ctcaactatg ttgtcattaa
     5821 tgatagtacg gacgagttct ttgtctttat agttcgcagg taattctatt ggttcttggt
     5881 tgtcgatacc aatagtaata tggtctgtat cattggagcc aatatcaata taagtaactg
     5941 aggaattatt aatattaaat cttaaggatc gtcgtttact cgtatggcgt tttttataag
     6001 ctcaagtaga tgtttaatac aggtgcttat tccttcctta ccattaaaaa ggtttttgag
     6061 ctcttgtact aatccattaa cttcatcatt gttattttca ttaatggatt ctgggtaagt
     6121 tgcgctggta ttgttgattg aaatagatgg tagcatgatt ttattccatt ttgattgatt
     6181 tgttacaaat atatattacc t
//
LOCUS       DAJTVJ010000199         6119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000199 DAJTVJ010000000
VERSION     DAJTVJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.200"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..358
                     /locus_tag="OO448_004057"
     CDS             <1..358
                     /locus_tag="OO448_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6339118.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="OO448_004058"
     CDS             complement(398..1069)
                     /locus_tag="OO448_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR6339119.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="OO448_004059"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="OO448_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6339120.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="OO448_004060"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="OO448_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6339121.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /locus_tag="OO448_004061"
     CDS             2798..3211
                     /locus_tag="OO448_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6339122.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="OO448_004062"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="OO448_004062"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6339123.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="OO448_004063"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="OO448_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6339124.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="OO448_004064"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="OO448_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6339125.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6119)
                     /gene="fliD"
                     /locus_tag="OO448_004065"
     CDS             complement(5600..>6119)
                     /gene="fliD"
                     /locus_tag="OO448_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6339126.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1497 a         1608 c         1527 g         1487 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gagataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttcttta
//
LOCUS       DAJTVJ010000200         5965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000200 DAJTVJ010000000
VERSION     DAJTVJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.201"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..202)
                     /locus_tag="OO448_004066"
     CDS             complement(<1..202)
                     /locus_tag="OO448_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6339127.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCN"
     gene            718..1287
                     /locus_tag="OO448_004067"
     CDS             718..1287
                     /locus_tag="OO448_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6339128.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            2015..2188
                     /locus_tag="OO448_004068"
     CDS             2015..2188
                     /locus_tag="OO448_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6339129.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OO448_004069"
     CDS             complement(2446..3774)
                     /gene="rutG"
                     /locus_tag="OO448_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6339130.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OO448_004070"
     CDS             complement(3795..4289)
                     /gene="rutF"
                     /locus_tag="OO448_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6339131.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(4300..4890)
                     /locus_tag="OO448_004071"
     CDS             complement(4300..4890)
                     /locus_tag="OO448_004071"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6339132.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OO448_004072"
     CDS             complement(4900..5700)
                     /gene="rutD"
                     /locus_tag="OO448_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6339133.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5708..>5965)
                     /locus_tag="OO448_004073"
     CDS             complement(5708..>5965)
                     /locus_tag="OO448_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6339134.1"
                     /translation="FADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYA
                     AINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1605 a         1533 c         1444 g         1383 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcattg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagcgt aaaaccaaat tcacggtgta
      121 agttcttaaa taacaacaat ggagttctcc agtaatcaga acccccatga tgtttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaaatttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagata agagatactt aacaggcttt ctaaacggat atcctttctg
      301 gaatatctat ctcttatctt aaaagtcttt acagatactt taaagatatc tgtattaaat
      361 ctgtacagta acttttcaga atctgaaaat ttttataaaa tcgtagtagc acctcttcaa
      421 attccccttc taaaaatctt cgaaaatatc tgacagatgt ctgtagctgc taagtacccg
      481 tcacaccagc tatgtgaccc cctttgcaaa aaagccaaat atcattgact ttctcggtga
      541 attgctataa aggcatctat ctaggtcttt aactgtataa atacacatga caacaaacgc
      601 ccgttaagtg tctatataaa aaggtagctg tagctataga caacaattgt gataattgat
      661 ttaatggcta tagacaagca agacagacct ttgttgacta tatagagaga taagaacatg
      721 cgtacattcc tttatgctcg tgtatcagtt gatgacctca ccacacagaa ccaagtagaa
      781 gctgttaaac gtgctggtta tgaagtcaga ccttcacgag taattgagga agttattagt
      841 ggggctacac cagctatgga cagaccacaa tttgtaaagc tggtggataa gctggaagaa
      901 ggtgattgtc ttgtagtgac caagattgac cgcttaggaa gggataacat tgacgtacag
      961 aagaccgtca caatgttact tggtatgggt gttaagctgg tctgtctaga tttgcctgtc
     1021 caagaccttt caagtgctga aggtaaactt attcttcaac tattcagtac ctttgcagag
     1081 tttgaactta accgcatcag agagcgaacc agagagggct tagaacgagc taaagcacaa
     1141 ggtaaggtgt tgggtcgtcc tgtagctaaa aacactacag aggccgtttt agagcataag
     1201 gctaagggat tgtcacaaag tcaggttgca aagcttctga atctctctgt gagaacggtt
     1261 agccgtcact ggacaaagac actgtaaaaa aaacttagta gcatcttcat agcctccttc
     1321 atggggactt ttttgtgtct gtaacatatg gtgaccaaac atctatatat cgttatatag
     1381 ccctgtatat agcactatat atagtgtctg gagagggacg actaacaccc tgcatagttc
     1441 cctgaaaagg ggcttcacag attctctata tatttcaata agttaggata aaaagggact
     1501 ataccgatac tgcatagaat ctgtgtagga ttgcatagtc ataggggttg gaacaagccc
     1561 aactacacct gataaagcct gttattaaca ggtagttgaa tgtacgtgtt tagctatgta
     1621 aaagcaccag aactttagcc atttctaacc actcctcgtg ttatctctat tccgtagcga
     1681 ttcgctacca cttatttaaa gataagacgt ccttttcaga gtgcaaattt cacaaccact
     1741 tatttgattt ataacaactt tcacaagcac gtaattttgt cgcaaaatga cacattttta
     1801 tctcatcgcg tttttttaat cataagagcg gcttatggat aattattgga gatgatatct
     1861 attctcgcta agaagctgtt gcaggatatt accaaacgcg ggtctacccg cgtcagttca
     1921 ctaagcttag tcccacgtag cgaaaatatg gcagccgcca tacgccgcgt taattctatg
     1981 caatatgatg tctataccca gacggaggtc agtaatggca aaccatcgag gcggttccgg
     2041 caattttgca gaagaccgcg aaagagcatc agaagcaggt aaaaaaggtg gacagcacag
     2101 cgggggtaat ttcaagaatg acccgcagcg cgcatctgaa gcaggtaaaa aaggtggcaa
     2161 gagcagtcac ggcaaaagcg acaactagcc gggctaatca atgacgaatg catttttgtc
     2221 tgtagctcgt caaaaagcca tcaccgccgg ttacccggtg gttgatactg atgacaaatg
     2281 taagcttgcc tgatgcgcga tgcttatcag gcctaccaga agattgcaat atattgaatt
     2341 tgcactgttt tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa caatgcgtac
     2401 gttgtcaaca atctgcaccg ccgggtaacc ccggcggttt tctgtttatg gctcctgatg
     2461 aacaacttct ggcggtggaa cgtcaaccaa ccttctactt aacaacgcat tgagtaaaat
     2521 cgcgccaaag gtggctgtac cgatccctcc caacgtaaaa ccgcccagcg tgagagcaaa
     2581 atcacccgcg cccagcacta aggttactgc gaccataatc aaattaccgt tctggcttaa
     2641 atcgacacgg ttttgtaccc agatccttgc gcctgcgacg gcaatcagcc cgaacacaac
     2701 aattgatgca ccaccaatca ccgcggccgg aatggtatgg atcagcgcgc caaatttcgg
     2761 cgaaaagccc aacagcatgg cgatgacggc agcagcaaca aacaccagcg tcgagtagac
     2821 ttttgtcacg gccatcacac cgatattttc agcataggtg gtgacgccgc taccgccgac
     2881 agagccggaa agcatcgttg ccagaccatc accgacgaat gctcgcccca tatacgggtc
     2941 catattgcgt ccggtcatcc cggcgaccgc cttgagatga cctaagtttt ccgccaccag
     3001 aatcaccgcc acgggcgcaa tcagcatcat tgcctgacta ttaaaagcag gagtggaaaa
     3061 atgtggcaaa ccgaaccagg cagcatggct gacgagagta aaatcgacgg cttttcccag
     3121 ccctaaaacg ttggtcatca cgccatacag cagacaggcg acaattaaac ctacgagaat
     3181 caataaccgc tggatcatgc cacgggtaaa caccgccacc agcccaatac acagcaccgt
     3241 cattaccgcc atccagctat caaaggctga agccgataca cttttcactg cgataggcgc
     3301 taagttcagg ccaatcgcca tcaccaccgc acccgtcacc accggcggca ttagtcgttc
     3361 aatccagcgt gtaccaattt tcatcaccac caggccaatg acggtataaa ccagcccaca
     3421 ggcgataatc ccgcccagtg cgatgctgat attcgggtta atgccctgac cgttaaagcc
     3481 cgtcgcggca atcaccacgc cgacaaaagc cgcgctggag ccgagataac tggggacgcg
     3541 cccgccggta ataaagaaaa acagtaacgt gccgatcccc gacattaaaa tggaaagatt
     3601 gggatccagc cccatcagaa tcggcattaa caccgtcgcg ccaaacatcg ccaccgcgtg
     3661 ttgaacgccc attactgccg tctgagcaaa cggcaatcgt tcatccggcg cgaccacgcc
     3721 gctctctgta gaggtcgatt ttaactgcca gtgaggaaaa ccgaacattg ccatcagctg
     3781 tctccttaag gaggttaaca agcagggcgc atcagcgcgt gataactgcg atcgaaccac
     3841 accagcccgt agggtgtggt gtgacgatga atcgcttcga tggcgcaaaa cagaatgtcg
     3901 tgggtgccga cgctcaccac ctggctgatg cggcagtcaa acgaaaccag agcctcttcc
     3961 agttgcgggc atccggtcac acccgtctgc cagcgggcgg cggcaaagcg gtgttccatg
     4021 ggcgttttgc cgccaaaaag atttgaaagc ggctcctgcc cggcgctcag tgtatttaca
     4081 cacagcgttc gattttcatt gaataccggc cagacggacg ccccacgatt caggcacacc
     4141 agtaatgtgg gcggcgtgtc ggtcacgctg cagacggcgc tggcggtgaa cccggcgcgc
     4201 ccggatggac cgtccgtggt gatgatattg accgccgcgc ccatacagga catcgcatcg
     4261 cgaaaggttt gttgatcaac tatgttcata gtttgctcct tacaacagcc cgcaggcttc
     4321 ttcaaaggac agacgtggca ggcgcgcaaa aagtttgctg ctatcgccat agccgatatt
     4381 aatcagcaga ttgctcttca gcgtgctgcc cgcaaaaaag gcgtcgtcaa cgtgttgacg
     4441 gtcaaaaccc gacatcgggc cggtatccag tcccagcgca cggcaagcga cgatcagata
     4501 ggccgcctgc atggagctgt tgcgaaacgc tgtttcttcg gcaagttgtg ggctggaggt
     4561 aaaccaactg cgggcatcac cgtggggaaa cagtagtggt aaccgttcat aaaattcact
     4621 gtcccaggca acgatagcgg tgacgggcgc ggtcagggtt ttttgtagat tgccgctgga
     4681 aagcgccggg tgcagacgtt cttttccttc tacagtgcgg ataaacacga tccgtgccgg
     4741 agaacagtta gctgatgtcg gcccccattt tatcggggca taaagctccc gtaacgtctc
     4801 atcgctgacg ggtgtctccc gccagccgtt gtgagtgcgg gcatcggtga acagggtgct
     4861 aagcgcacct gggctaacgg cttcgttcat agcaattcct tacagggcgg cttcacggtg
     4921 atgtaacagg ctggcaagcc cgttgagtaa cagagcatta aacgtttcgg ggtcggtcac
     4981 gttgcaggcg tgtccgccat agcgcatcac cattttctgg ctatcgggca gggcggcatg
     5041 aagctcactg gaacatgctg atggcaccag cagatcatca ctggcgcaga tgatttgcac
     5101 cgggcagcgg atgcgatccg catggtgact aaagtcagcg cgtttgaggg cgttaagtcg
     5161 acgcagtaaa ttatttttgc cctgaaaatg cgccagtgcc agcgcgtctt ctgcctcaag
     5221 gcgaggtgcg cgggccgcca tccagtcggc gggatagagg aacaacggct gcgcttccac
     5281 ccatgcctgc gcgccgccgc tatacagtaa tcgttcgcga acctgaaaac agcggcgcgt
     5341 atgggcgttt attcgtagcc agccgttaac gctgatcagc acagttaccg acgcgggata
     5401 atccagcgcc agctgcattc ccaccagcgc accgagcgca tggccgacca ccgcgtaatg
     5461 ctcaatccct gcggctacca gcgcctgatg cagttccgct gccatctggg cgatactgta
     5521 atcttctgcc agcgtgtcgg gattattgcc ggtgccgcgc tggtcgtaac agactacctg
     5581 atactcctgc tccagcaccg ccagttgcgg taaccagtaa ctgccgctac cgccaagacc
     5641 cgaaatcaac accactacgg gcgcatcagc ataaggggga ggtgagagtg aaagtttcat
     5701 cgcggcctca cttggcgata tgcgcaattg tggcgatttc caccagcgcg tcaggtttta
     5761 ccagtccgca ctgaatgcag aatcgcgccg gtttatcacc cggaaaaaac tcggcgtaga
     5821 tttcgttaat cgcggcgtaa tttttccagt cggtaataaa gatgctgttg aaggtcacat
     5881 ccgccatcgt gccacccgcc gtctcgatca ccttgcggat agtttccaga acgtggcggg
     5941 tttgcgcctt tgggtcatcg gcaaa
//
LOCUS       DAJTVJ010000201         5927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000201 DAJTVJ010000000
VERSION     DAJTVJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.202"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            282..358
                     /locus_tag="OO448_004074"
     tRNA            282..358
                     /locus_tag="OO448_004074"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Arg,seq:tct)
     gene            complement(403..1164)
                     /gene="envY"
                     /locus_tag="OO448_004075"
     CDS             complement(403..1164)
                     /gene="envY"
                     /locus_tag="OO448_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6339135.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1347..2237)
                     /locus_tag="OO448_004076"
     CDS             complement(1347..2237)
                     /locus_tag="OO448_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6339136.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2238..5210)
                     /gene="nfrA"
                     /locus_tag="OO448_004077"
     CDS             complement(2238..5210)
                     /gene="nfrA"
                     /locus_tag="OO448_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6339137.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5197..>5927)
                     /gene="nfrB"
                     /locus_tag="OO448_004078"
     CDS             complement(5197..>5927)
                     /gene="nfrB"
                     /locus_tag="OO448_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HCR6339138.1"
                     /translation="QLDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWES
                     IDAWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKV
                     RYFIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLF
                     AEILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSM
                     QSLLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1345 a         1558 c         1550 g         1474 t
ORIGIN      
        1 aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc tcataggagc
       61 ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac gggttttctt
      121 gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa tcagaaaatc
      181 agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca gcaagggttg
      241 accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta gctcagttgg
      301 atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg gcgcgccatt
      361 tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca tcactttttg
      421 tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa aaacagagat
      481 aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat ttttgttatc
      541 catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct ggctatagct
      601 gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata gcgaactggc
      661 gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc agacgctgtc
      721 gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt gctcaagaaa
      781 gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg gtttgctggg
      841 ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc tatgacaagc
      901 gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct tatttaaaaa
      961 caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt gatttaacgt
     1021 tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt tcggaagggt
     1081 aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga tcaccacgca
     1141 aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact ccgctcaaag
     1201 gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt ctttctttag
     1261 cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag gtaagccagg
     1321 tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc gacgggcaaa
     1381 tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc taacagcgag
     1441 gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc ggcgacaaac
     1501 agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct ggcctgtaga
     1561 taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg tacccagaca
     1621 ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg cgacatgttt
     1681 ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat cagccgctgc
     1741 gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca gttcaggtcg
     1801 tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc gctccataat
     1861 ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag cgctgcggac
     1921 gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat cagtttcagg
     1981 ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc tggctgggta
     2041 aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg caaacgtaac
     2101 tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt ttgtggttgc
     2161 cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt cagcaatacg
     2221 aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt gttgcgctct
     2281 ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag actgactttg
     2341 tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac cccgactccg
     2401 ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac gtgagcgtaa
     2461 ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata atctgccgtc
     2521 cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt ttgggcaaac
     2581 cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa gaatgaggcg
     2641 ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa ctgctgttcg
     2701 acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt gccggacatc
     2761 ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac gcggctataa
     2821 actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc ggcttccagt
     2881 tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt gttagcggta
     2941 ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca gcggcgaccg
     3001 acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg ggtcagtggg
     3061 gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg ctgcgtcgca
     3121 ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc cggatcgtcc
     3181 ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc accgctatcc
     3241 cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag ttccagcgcg
     3301 gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat tgtcgcccgc
     3361 gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc gttcagtgcg
     3421 agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag ggcattgctt
     3481 cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc atttcctgcc
     3541 gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg aagactgatt
     3601 ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg ataggctacc
     3661 gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc atacaacgcc
     3721 acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc agcatcgtag
     3781 gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc aatacccggc
     3841 aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa aatcgccacc
     3901 ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg cgccattaaa
     3961 gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa atcggcagct
     4021 tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg ggtaagattt
     4081 gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt gttacgggtc
     4141 gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt ggcgagcagt
     4201 ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc gccaacaaca
     4261 tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac ggggttggcg
     4321 ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt aaacagcggc
     4381 ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc cagcgcggtc
     4441 gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag tgctaggatc
     4501 cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc tgccgcgctt
     4561 aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca ttgtttcagg
     4621 tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga tgcagcaaac
     4681 gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag ggcattctgc
     4741 ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg ctgggcaagc
     4801 agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag actgcgctca
     4861 agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg cgcccggtca
     4921 tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat attatccggc
     4981 acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt atttttctgt
     5041 gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc gctcagcccc
     5101 agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa taaagacgtc
     5161 agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat tttcggactc
     5221 cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt gcatcgaaac
     5281 ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga taacgccttc
     5341 ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata acaacacgtt
     5401 aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca ggaactgatg
     5461 cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact gatgctgaac
     5521 cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc agtgacgtaa
     5581 ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc cgactttacg
     5641 cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa ttaactcatc
     5701 attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg gcatttcggc
     5761 aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc cgttttgctc
     5821 tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca ttgaaccgcc
     5881 caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgtt
//
LOCUS       DAJTVJ010000202         5837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000202 DAJTVJ010000000
VERSION     DAJTVJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.203"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO448_004079"
     CDS             complement(<1..242)
                     /locus_tag="OO448_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6339139.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="OO448_004080"
     CDS             complement(604..3387)
                     /locus_tag="OO448_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6339140.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="OO448_004081"
     CDS             complement(3444..5816)
                     /locus_tag="OO448_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6339141.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1487 a         1321 c         1312 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttgcgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacagc
//
LOCUS       DAJTVJ010000203         5729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000203 DAJTVJ010000000
VERSION     DAJTVJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.204"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1934)
                     /gene="speC"
                     /locus_tag="OO448_004082"
     CDS             complement(<1..1934)
                     /gene="speC"
                     /locus_tag="OO448_004082"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6339142.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SADMTFRVKPESLILTIFFLG"
     gene            2336..3043
                     /locus_tag="OO448_004083"
     CDS             2336..3043
                     /locus_tag="OO448_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6339143.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3149..3224
                     /locus_tag="OO448_004084"
     tRNA            3149..3224
                     /locus_tag="OO448_004084"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3182..3184,aa:Phe,seq:gaa)
     gene            3422..4393
                     /locus_tag="OO448_004085"
                     /pseudo
     CDS             3422..4393
                     /locus_tag="OO448_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            4531..5550
                     /locus_tag="OO448_004086"
     CDS             4531..5550
                     /locus_tag="OO448_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR6339144.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT         1529 a         1452 c         1430 g         1318 t
ORIGIN      
        1 cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt catatcagcg
       61 ctatgcgcat cctgggggtt catcaccact gacgggaaac tctgctggcg gaacatcgct
      121 ttttgtaggt ctttgacgtc gaaactgaca tacagatcgt gcatctcctg acacaactgg
      181 cgcagggtgt agtcgcgata gcgcaccgga tatttgttat aaacgctcgg caacacctca
      241 gccagcggcg agtcatcctc aatatgctgt tcaaattgcg ccagcatcgc caccagttgt
      301 gccagcttct cgtggctttc cgccggagtt aataagaaca gaatggagtt gagatcgcac
      361 ttctccggca caatgccgtt ctcacgcaga tagtgcgcca gaatcgtcgc cggaacgcca
      421 aagtcgctat attcgccggt ttcggcattg atacctggtg tagtgagtaa cagcttgcac
      481 ggatcaacaa aatactgatc cgcggcatat ccttcaaagc cgtgccactt cgcccccggc
      541 tcaaaactga aaaaacggcg gtcgctggct aacacagatg tcggataatc ctgccacaat
      601 ttgccatcaa caacgggcgg gataaacggg cggaacagct tacagcgcgc aagaatagcc
      661 ttgcgcgact caatacccaa ctcaacgcac tcagcccaca gccgacgccc actctcccct
      721 tcatgaattt tggcgttaac atccagtgca gcaaacagcg gatagaaagg gctggtagaa
      781 gcatggagca taaaggcgtt attcaaccgc ttatgcgggc aaaaacgcgc ctgtccgcgg
      841 atatggttat cttttttatg gatctgcgac gtctgtgaga atcccgcctg ctgtttgtgc
      901 accgactgag tcacaaagat ccccggatcg ttttcgttaa gttctaatag cagcggcgag
      961 ctatccgcca tcatcgggat aaactgttcg taaccgaccc acgcggaatc aaacagaatg
     1021 taatcacaca gatgcccaac ggtatcgatc acctgacggg cgttatagac agttccgtca
     1081 taggttccca gctgaataat cgccaggcga aacgggcgcg gcagctcggc tttttctggc
     1141 gcaacgtcgc gaatttgctg gcgcagatac tcttcattaa aacagtgcgc atcaataccg
     1201 ccaatgaaac caaacgggtt gcgtgaagct tccagataga ccgacgtcgc ccccgcctga
     1261 atcagcgcgc cgtgatgatt cgacttatgg ttgttacggt cgaagagcac cagatcgcca
     1321 cgcgttaaca gcgcattcgt caccacttta ttcgctgccg atgtgccgtt cagcacaaaa
     1381 taggttttat cggcatgaaa gactttggct gcgaatttct gcgcatcctt cgccgatcct
     1441 tcatgaataa gcagatcgcc caattttacg tcagcgttac acatatcggc gcgaaagacg
     1501 ttctcaccaa agaaatcgta aaaatggcgt ccggcaggat gctttttaaa aaacgcacca
     1561 tgttgatgtc cagggcaagc aaaggtgctg ttgcccatct caacgtactg cgtcagcgtg
     1621 tcataaaacg gtggcagcaa attctcttca tactgacagg ctgcggattc cagctccagc
     1681 cactgctgct cgttgccgtt gattaccgcc gtaacgcccg caggtaattc aacagcatgt
     1741 tcggaataca aaaacaccgg tagatgaaaa ccggtgcgct taagcaacgc aagaatgcca
     1801 ctgcgactat ccgcagcggt aatgacgact gccgcgacat ccgtaaaatc agtatctccc
     1861 aacgccacca cgcggcgatg agtagaaagt cgggatacca gttcactact ggcggcaata
     1921 ttcattgatt tcataagcgc aaaacccgtt tcggggaagt aagaataccg gacaaagtgt
     1981 aaaaccctgc cccatgagat atgggtcaaa ctggtcacca gctccgacga ccgccagaca
     2041 tcagtaaaag cagaaacgct ctgattttac tgttgtcctg cagtgaacgt gcgttaactt
     2101 caccgcatga gcagtaacat agaaagggaa acgtttcgcg cgaaacggcg ataagcgaga
     2161 gaatgtaagg agatggcgtg catcgggcaa actccgtaca gagaggagaa aattcgcgca
     2221 atcatggcac ctttcgctaa ggcgtgcaag ccaggacttt gcgaccagac aagccatcaa
     2281 caaggcaaaa agttcataat aagaaaatca aacaatacag ctaacaggaa ctcttgtggt
     2341 catcggccct tttatcaacg ctagtgcagt cttactgggt ggcgttctcg gcgcactgct
     2401 cagccaacgc ttaccggaac gtatccgcgt ctccatgaca tctatttttg gtctggcatc
     2461 gctggggatt ggtattttac tggttgtgaa atgcgccaac cttccagcga tggttttagc
     2521 tacgctactt ggcgctctaa ttggcgaaat ttgtttgctg gagaaaggtg tcaatacagc
     2581 agtcgccaaa gcacaaaatc tgtttcgtca ctcacgtaag aagccagcgc atgaatcttt
     2641 tattcagaat tatgtcgcga ttattgtcct gttttgcgcc agcggcaccg ggatcttcgg
     2701 ggcgatgaac gaagggatga ccggcgatcc gagtatttta atcgccaagt catttcttga
     2761 tttctttacg gcgatgatct tcgcctgctc gctgggtatt gcggtgtcgg taattagtat
     2821 cccattactg atcatccagc taacgctagc gtgggctgcc gcgctgatat taccgctgac
     2881 cacaccgtcg atgatggcag acttcagcgc cgtaggcggt ttattgctgc tggcaaccgg
     2941 attacgcatc tgtggcatta aaatgttccc ggtggtcaac atgcttccag cactccttct
     3001 ggcaatgccg ctttccgccg cctggaccgc ctggtttgcc tgacaatgcg tgcaatagcg
     3061 gcaaagtgat gatagattgt gcagtctgca gtaaattgaa gaaatttgat tgacgagacg
     3121 aggcgaatca ggtttaatgc gccccgttgc ccggatagct cagtcggtag agcaggggat
     3181 tgaaaatccc cgtgtccttg gttcgattcc gagtccgggc accaaattca tatcaacgga
     3241 cctccacgga ggtccgtttt tcgtttcaga acgccacgat ttaagcgttc tgcctacaaa
     3301 tcaattctac cgaactcaac cagattcccc ccacatcaac cccattgtgt gggtataatt
     3361 gcgggcatac cccagttcga cagaatttgt accctcttta acgcattcag aaggctgaac
     3421 tatggcactg actgacgcaa aaatccgggc tgcaaagccc actgacaagg cttataaact
     3481 cactgacggg gctggcatgt tcctgctggt acatcctaat ggctcccgtt actggcgtct
     3541 ccgttatcgt attctgggta aggagaagac tctggcactt ggtgtgtatc cagaagtttc
     3601 tctctccgaa gctcgtacaa aacgggatga ggcccgaaaa ctgatttcgg agggggttga
     3661 cccttgcgaa cagaaaagag ctaaaaaagt agtccctgat ttacagctct cttttgaaca
     3721 tattgcgcga cgctggcatg ccagtaataa acaatgggca caatcacaca gcaataaagt
     3781 actcaaaagc cttgagactc acgttttccc ctttatcggc aaccgggata tcacaacact
     3841 caataccccg gatctgctta tccctgttcg tgctgcagaa gctaaacaaa tttatgaaat
     3901 cgccagtcgt ctgcagcaaa gaatatctgc cgtaatgcgt tatgccgtac agtctggcat
     3961 catcagatat aatcctgctc tggatatggc tggcgcattg actacggtaa aacgccagca
     4021 tcgccccgct cttgatcttt ctcgcctgcc tgaacttttg tcgcgtattg gcagttacaa
     4081 agggcaacct gtcacccagc ttgccgttat gctgaattta ctggttttta ttcgttccag
     4141 tgaactcaga tacgcccgct ggtctgaaat tgatattgac aatgccatgt ggactattcc
     4201 agccgaacgc gaacccctgc caggcgtaaa attctcacac cggggctcca agatgcgaac
     4261 accacatctt gtgccactca gcaaacaggc tgtagccata ctgacagaac tacagacatg
     4321 ggctggtgaa aatggtctga tctttacggg agcacatgac ccgcgtaaac caatcagtga
     4381 aaatgtaatg aacgcatccc acacaattaa acacggagca gtatgatggt taaaagggtg
     4441 aaactggcag gtgtggagcc tcgcagattt ccggcatcat agtgcccaat acgggatgca
     4501 taaagctccc tcagactgga gactccacaa atgaaatata caccggttgg cgttgatatc
     4561 gcaaaacatg tcattcagat tcacttcatc aatgagcaca caggtgaagt ggttgataaa
     4621 cagttgcgta gacaggattt tctgacgttc ttcggcaacc gtgagccatg cctgattggt
     4681 atggaggcct gtggaggttc tcagcactgg gcacgggaac tgacaaaact tggtcataaa
     4741 gtccggttgt tgcaggcccg cttcgttaag gcattcgtca tgggcaataa gaatgatgtg
     4801 atggatgccc gggctatctg gatggcggtt cagcagccgg gtaaagaaat cgccgtaaaa
     4861 acagaagaac agcagtcggt actggttctg caccgtaccc gcatgcaact ggtgaagttc
     4921 cggaccgcac aaattaatgc cctgcacggg acgttactgg agtttggtga aaccatccac
     4981 aaaggccggg cagcgatgga gcgggagttc cccgaagcac tggaacggat gaaagagaga
     5041 ctgccaccgt atctcattat ggttctggaa aaccagtaca accgactgaa tgagctggac
     5101 tcactgatag aggatattga aaaacagctt accagcgtgg cgaggcagaa tgaaacctgt
     5161 aagcggttgc tggatattcc tggcgttgga ccacttattg cgacggcagc ggtggccacc
     5221 atgggggaag catcagcgtt taaatcgggg cgagagttcg ccgcatatgt tggtctggtt
     5281 ccaaaacaaa ctggctccgg agggaaagta cgtctgctgg ggataagcaa acgtggtgac
     5341 acttatctca ggacattatt tatccacggt gcaagagcgg tggcattagt agctaaagag
     5401 cctggcccgt ggataaccga actgaaaaaa cgtcgtccag ccagtgtggc aatcgtcgcc
     5461 atggcaaaca agctggcacg aacagtatgg gcgataaccg cccatgaccg taagtatgac
     5521 aggaaccacg tcagtatcag accatattaa tcgctgatac cattaaacaa tgaactctta
     5581 acaaaagggt gaatgctgaa aggttgctat ggcggccaga gtgatgacaa agacaggtaa
     5641 gaccgtgact cactaaacct gaacagtatt ttgggcttga agtccgccgt gaaaataagg
     5701 ggtgagtcgg cgaattacat aggggctcg
//
LOCUS       DAJTVJ010000204         5640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000204 DAJTVJ010000000
VERSION     DAJTVJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.205"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO448_004087"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO448_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6339145.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO448_004088"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO448_004088"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6339146.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO448_004089"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO448_004089"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6339147.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO448_004090"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO448_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6339148.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAJTVJ010000205         5616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000205 DAJTVJ010000000
VERSION     DAJTVJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.206"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="OO448_004091"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="OO448_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6339149.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="OO448_004092"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="OO448_004092"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6339150.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="OO448_004093"
     CDS             2475..4124
                     /locus_tag="OO448_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6339151.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="OO448_004094"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="OO448_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1257 a         1270 c         1456 g         1633 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgcat
//
LOCUS       DAJTVJ010000206         5531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000206 DAJTVJ010000000
VERSION     DAJTVJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.207"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1116
                     /locus_tag="OO448_004095"
     CDS             <1..1116
                     /locus_tag="OO448_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6339152.1"
                     /translation="LRDIEVEAVTKMLACGTRILGVKEYNCDKPECPHVRYVTNSCGS
                     RACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLAVE
                     NLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGCLNKHGQWKKLSFLKDAMR
                     SRWMWNMRQRLLKAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSKKTAGGR
                     NTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELVARLKQH
                     IPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYVQMVKQFLSRDPFE
                     CVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
     gene            complement(1508..1570)
                     /locus_tag="OO448_004096"
                     /pseudo
     CDS             complement(1508..1570)
                     /locus_tag="OO448_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            2021..3433
                     /gene="ospC1"
                     /locus_tag="OO448_004097"
     CDS             2021..3433
                     /gene="ospC1"
                     /locus_tag="OO448_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6339153.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSIANQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(3537..3686)
                     /locus_tag="OO448_004098"
                     /pseudo
     CDS             complement(3537..3686)
                     /locus_tag="OO448_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3762..5459
                     /gene="ospD3"
                     /locus_tag="OO448_004099"
     CDS             3762..5459
                     /gene="ospD3"
                     /locus_tag="OO448_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6339154.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1815 a         1042 c         1184 g         1490 t
ORIGIN      
        1 ctgcgcgata tcgaggttga ggctgtcaca aaaatgctgg cctgcggcac gcggatactg
       61 ggagtgaagg aatacaactg cgataaacct gagtgccccc acgtcagata cgtcactaac
      121 tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg ccacagacct gtggatagcg
      181 acacagctga atcgtcttcc tgactgcgac tgggtacatc tggtcttcac cctgccggac
      241 acgctgtggc cggtgttcga aagcaaccgg tggctgctga atgacgtgtg ccgtctggcg
      301 gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg aacccggtat cttctgcgcc
      361 atccacacgt atggccgtcg tctcaactgg catccgcatg tacatgtgtc tgtaacctgt
      421 ggatgtctga ataagcatgg tcagtggaaa aagctgagct tcctgaaaga cgcgatgcgt
      481 tcgcggtgga tgtggaatat gcggcaacgg cttctgaaag cgtggtcaga ggggctggcg
      541 atgccggagt cgttgtcaca tatcacgacg gaatcacagt ggagaagcct ggtgctgaaa
      601 gccggaggaa aatactggca tgtgtacatg tcgaaaaaaa cggccggcgg gaggaatacg
      661 gcgcgctacc tgggtcgtta tctgaagaag ccgccgatag cggcctcccg gctggcacat
      721 tacaacggag gggcgagcct gagcttccgt tacctggacc acaaaacggg agaaacggcg
      781 acggaaacgc tgacacagcg tgagctggtc gcgaggctga aacagcacat cccggagaag
      841 ttttttaaga tggtgaggta cttcgggttc cttgccaacc gggtgtgtgg agagaagctg
      901 ccgcaggtgt accgtgctct ggggatggat aaaccggaac cagtggcgaa agtgtgctat
      961 gtacaaatgg tgaaacagtt cctgagtcgt gacccgttcg aatgcgtgct gtgtggcggc
     1021 cggatggtat accgccgggc cattgcgggg ctgaatgtgg aggggctgaa gaaaaacgcg
     1081 cgggatatca gtctgctgag gtatatgccg gcctgacaca ggctaagtgt gcccgggaat
     1141 cggtgcaagt ataaaaaaca gcctgttgcg gtataagaat cgaccatgca gagagagaaa
     1201 acaccggagt ggtggggaaa aacagaaagg ctcacgtggc ataaggcgcg gacaggggta
     1261 tcgcttagtt tttcaatttc ctatacgtga acgcccgcct gcgggcactg ttgcagacgc
     1321 aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg
     1381 cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgtagt
     1441 tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg
     1501 cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact
     1561 cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc
     1621 tctattaaaa aaacatcagt ttttcacact tagttgagct tcatgaaaaa atcatcgagg
     1681 atttcattgc agaatacaca ttccactaac tggaagcact ccataagagg aatcagagaa
     1741 agtatcgttt ttttatagta aaattcattg ctgttcaata aaattgatat agttattaaa
     1801 ttatgaaaat aatttcacaa aaaagattaa cttgagttct taagcatcgg attttagata
     1861 ttttgaaata tctggcatca cataacatca aaagtatata acacccttat ctaattacat
     1921 gcagtatatt gtatatatac tcaaaaattg taattcatac cctgctatat cttgtaggga
     1981 aagatattaa ttaaaactgt tttcatataa ggttcatttt atgaatatat cagaaacact
     2041 gaactcagca aatacccaat gcaatataga ttctatggat aacagattac atacattgtt
     2101 tccaaaagtg acatcagtgc gaaacgctgc acaacaaact atgccagatg aaaaaaattt
     2161 aaaagatagt gcaaatatta ttaaagattt ctttaggaaa actatagcag cacagagtta
     2221 tagtagaatg ttctctcaag gctctaactt taaatcttta aatatagcaa ttgatgcacc
     2281 atcagacgct aaagcctcat ttaaggctat tgagcacctt gacagattat cgaagcatta
     2341 tatatctgaa ataagggaaa aacttcatcc tctttctgca gaggaactca atttgctttc
     2401 gctaattatt aattctgatt taatcttcag acatcaaagt aattctgatt tgtctgataa
     2461 aattttaaac attaagtcat tcaataaaat tcagtctgaa ggaatatgca caaaacgaaa
     2521 cacatacgct gatgatataa aaaaaatagc taatcatgac tttgtgtttt ttggcgttga
     2581 aatctctagc catcagaaaa aacaccccct gaatacaaaa catcacactg ttgattttgg
     2641 tgcaaatgcg tatatcattg atcatgactc tccatatgga tatatgacat taaccgatca
     2701 ctttgataat gctattccac ctgtttttta ccatgagcac caatcatttt tagataaatt
     2761 ttcagaggtt aataaagaag ttagtcgata cgtacatgga agtaaaggaa ttatagatgt
     2821 accaatattc aatactaaag atatgaagtt agggctcgga ttatacctga ttgactttat
     2881 tagaaaaagt gaagaccaaa gcttcaagga gttttgctat ggaaaaaatc ttgcccctgt
     2941 ggatctggat agaatcataa actttgtttt tcagccagag taccatatac ctaggatggt
     3001 aagtacagaa aacttcaaaa aagttaagat tagagaaata tccttagagg aggctgttac
     3061 agcatctaat tacgaagaaa ttaacaagca ggtcactaac aaaaaaattg ctctccaggc
     3121 tctttttctt tcgattgcta atcaaaaaga ggatgtcgcc ttatatatat tatctaattt
     3181 tgagataact agacaagatg ttatttccat aaagcatgag ttgtatgata ttgagtatct
     3241 acttagcgct cataattcaa gctgtaaagt acttgagtat tttatcaata agggattggt
     3301 tgatgtaaac acaaagttca aaaaaactaa tagtggggat tgtatgttgg ataacgcaat
     3361 aaaatatgag aatgcagaaa tgataaaact attattgaaa tatggtgcaa catctgacaa
     3421 taaatatatt taatcaaaat tgaatatcgt ttagcaaaag gcagtatttg cacaaaatat
     3481 cgatatattg tttattgtca gtatggctca atgtgataat agttggaaag tttgatgggt
     3541 ttcgccccgt tgtagcggta gtcgagctgg aaggtcttca ggcactgctt acagcgatag
     3601 agcagccccc ctgaactgga atggccgttc cggatacccc cctgagtttc agagtaacgg
     3661 ggacaaacca catcaatctt tgccatcaat catccaaagg gcaaagagta caacaacact
     3721 aagtctgcgt cacaacccat caatgaaagg aatatataca tatgccatca gtaaatttaa
     3781 ttccatcaag gaaaatatgt ttgcaaaata tgataaataa agacaacgtc tctgttgaga
     3841 caatccagtc tctattgcac tcaaaacaat tgccatattt ttctgacaag aggagttttt
     3901 tattaaatct aaattgccaa gttaccgatc actctggaag acttattgtc tgtcgacatt
     3961 tagcttccta ctggatagca cagtttaaca aaagtagtgg tcacgtggat tatcatcact
     4021 ttgcttttcc ggatgaaatt aaaaattatg tttcagtgag tgaagaagaa aaggctatta
     4081 atgtgcctgc tattatttat tttgttgaaa acggttcatg gggagatatt attttttata
     4141 ttttcaatga aatgattttt cattccgaaa aaagcagagc actagaaata agtacatcaa
     4201 atcacaatat ggcattaggc ttgaagatta aagaaactaa aaatgggggg gattttgtca
     4261 ttcagcttta tgatcccaac catacagcaa ctcatttacg agcagagttt aacaaattta
     4321 acttagctaa aataaaaaaa ctgactgtag ataattttct tgatgaaaaa catcagaaat
     4381 gttatggtct tatatccgac ggtatgtcta tatttgtgga cagacatact ccaacaagca
     4441 tgtcctccat aatcagatgg cctaataatt tacttcaccc caaagttatt tatcacgcga
     4501 tgcgtatggg attgactgag ctaatccaaa aagtaacaag agtcgtacaa ctatctgacc
     4561 tttcagacaa tacgttagaa ttacttttgg cagccaaaaa tgacgatggt ttgtcaggat
     4621 tgcttttagc tttacaaaat gggcattcag atacaatctt agcatacgga gaactcctgg
     4681 aaacttctgg acttaacctt gataaaacgg tagaactact aactgcggaa ggaatgggag
     4741 gacgaatatc gggtttatcc caagcacttc aaaatgggca tgcagaaact atcaaaacat
     4801 acggaaggct tctcaagaag agagcaataa atatcgaata caataagctg aaaaatttgc
     4861 tgaccgctta ttattatgat gaagtacaca gacagatacc cggactaatg tttgctcttc
     4921 aaaatggaca tgcagatgct atacgcgcat acggtgagct cattcttagc ccccctctcc
     4981 tcaactcaga ggatattgta aatttgctgg cctcaaggag atatgacaat gttcccggac
     5041 ttctgttagc attgaataat ggacaggctg atgcaatctt agcttatggt gatatcttga
     5101 atgaggcaaa acttaacttg gataaaaaag cagagctgtt agaagcgaaa gattctaatg
     5161 gtttatctgg attgtttgta gccttgcata atggatgtgt agaaacaatt attgcttatg
     5221 ggaaaatact tcacactgca gaccttactc cacatcaggc atcaaaatta ctggcagcag
     5281 aaggcccaaa tggggtatct ggattaatta tagcttttca aaataggaat tttgaggcaa
     5341 taaaaactta tatggaaata ataaaaaatg aaaatattac acctgaagaa atagcagaac
     5401 acttggacaa aaaaaatgga agtgattttc tggaaattat gaagaatata aaaagctgaa
     5461 tattatctcg tggtgataca ctgaatttga ggtaaccggc tcatttaaac cgtctggtct
     5521 gttacctccg g
//
LOCUS       DAJTVJ010000207         5480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000207 DAJTVJ010000000
VERSION     DAJTVJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.208"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            526..1652
                     /gene="ltrA"
                     /locus_tag="OO448_004100"
                     /pseudo
     CDS             526..1652
                     /gene="ltrA"
                     /locus_tag="OO448_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1735..1914
                     /locus_tag="OO448_004101"
                     /pseudo
     CDS             1735..1914
                     /locus_tag="OO448_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1944..2426)
                     /locus_tag="OO448_004102"
                     /pseudo
     CDS             complement(1944..2426)
                     /locus_tag="OO448_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2682..3662)
                     /locus_tag="OO448_004103"
     CDS             complement(2682..3662)
                     /locus_tag="OO448_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6339155.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIHLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3662..4861)
                     /locus_tag="OO448_004104"
     CDS             complement(3662..4861)
                     /locus_tag="OO448_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6339156.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5183..5480)
                     /locus_tag="OO448_004105"
                     /pseudo
     CDS             complement(5183..5480)
                     /locus_tag="OO448_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1519 a         1244 c         1292 g         1425 t
ORIGIN      
        1 cagggtttac aggagcactg accaaacgac ccggtgctgc ttcattctaa ggaatgaggt
       61 ggtgagcgac tcgggaaaag gcagagatct actgtcacgt ggggttcggg aagctgaacg
      121 aaagtaaact gtccgatgaa gcatcgaaat cttagatgat gtcaaaatgg ggccgtacag
      181 ccgccccctg ataagcgcag aagacacctg cttactgact gcgcggcatc cggtgtgaag
      241 gcagcaggat cccgggccag accatctcag ggaacgtgag aacctgtttc cggcacgtat
      301 cgggaaacag gggcggaccc attcgtagta gtgatgaaag atctgtaatg ggtctggagc
      361 gaagggatgg ggatcacatt cattgcagaa gacacaaccc aaaaggggat gacgtaactg
      421 atgcaacgaa aatcatttga aataccaaaa gcactggtct gggcatcata tctggatgtg
      481 cgtcgcaaca agggagcccc cggatgcgac gggcagactt tgaaaatgtt cgaccagcaa
      541 cgtgatggca atctatacaa gatctggaac cggctatgtt cgggaacatg gtttccgcca
      601 ccagtgctgg aaaaacggat cccgaagtcg aatggcaagg aacgcattct ggggatccca
      661 acagtatccg atcgaattgc tcagggagcg ataaaacttt tcatggaaga aaagcttgac
      721 ccgattttcc atgctgattc atatggttac cgaccaggca aatcagctca tgacgctctg
      781 aaacaatgtg ccatccggtg ctggcgttat agctggatac tggaagtgga catcagtgcg
      841 tttttcgacc atgtgagaca tgatctggtg ctcaaagctc tggaacacca cgggatgcct
      901 aaatgggtca tcctgtactg tcgacgctgg atggaagcgc caatgcagag ttgtgaaaat
      961 ggagaattaa taacccgaac acgaggcact ccgcagggcg gggtcattag tccgctactt
     1021 gctaacttgt tccatcacta tgcatttgat ttgtggatgg aaagagaata tcgggggtac
     1081 cgtttgagag gtacgctgac gatattgtag tacattgttc acgaatgagt gatgcaacaa
     1141 ggctgaagaa caggttatcg gagcgcttct cggaagtcgg gctggtcctg aatgcaggga
     1201 aaacgaatat cgcctacatt gacacgttta aaaggagaaa cgtcgcaacg agtttcacct
     1261 ttctcggata tgacttcaaa gtgcgtacgc tgaagaattt caaaggcgaa cggtaccgaa
     1321 aatgcatgcc gggtgcgtca aatgcagcaa tgcgcaaaat aacagaaaca atcaagaagt
     1381 ggcgtataca tcgctcaaca gctgagagtt tgctggattt tgcgaggcgc tacaatgcga
     1441 tagtgagagg ctggatcgag tactacggaa agttctggtc cagaaatttc aactatcgac
     1501 tgtggagtgc aatgcagtca cgtctgctca agtggatgca gtctaaatac agactttcga
     1561 accggaaggc tcagcgaaag ctgacgctgg taaggaagga gtatccgaag ctatttgttc
     1621 actggtattt actacgtgca tcaaatgagt gatcaagagc cgtatgacgc gagagtgtca
     1681 cgtacggttc cgtgagcagc cggggagtga aattctctcc ggctgactcg actcgcgggt
     1741 tccgacagcg atgacgaaca tgcggcggtg ttgtactcgc tgatcggcac atgccgtctg
     1801 aacaatgtgg aaccagaaaa atggctgcgt tacgtcattg agcatatcca ggactggctg
     1861 gcaaaccggg tacgtgatct gttgccctgg aaagttgatc tgagctctca gtaaatatca
     1921 atacggttct gacgagccgc ttacttttgg cgtaaatctg ctgcgccttt actgaacaca
     1981 atgccgtatt cagcaagaag tccacgaatg tgatttatca gttgtgtcct ttccattgaa
     2041 accaaccgac gcatattcaa aaaagtctgg acgtcctgtt gctccagagt ttttattggt
     2101 acgggtcgta tagttccatg ttggcaggcc tcagctattg cctgtgcgtc gttataatca
     2161 tttttctggc accgctgata agctttaaca tgttgtgccg gaagcaatat tacatcatgg
     2221 tcgagtttcc tgatttcccg ggcccagtaa tgagatgccc cgccagcctc catcgcaaca
     2281 gtcattttag gctgttgcac cagccagtta agcagttgat tgcgagaaag tttggtcttt
     2341 cctgcgggtt tgccatgagc attgatactg aaaagataaa agttcgtttt agccagatca
     2401 atgccggtga ttttctgttc agacatggta ttccgcttac ttttgtggtt aaagtttatc
     2461 catcatggcg caattgaagg ctgccgtgaa agagggggaa gactatctca tcggttactg
     2521 ttaaacggct aagagaaact atctttttca agcaaagaag atacggtgaa acaggcgatt
     2581 tcaccgtgaa atactgttca aaaaaaacgc taataaaagc aaaatttctt ttaaaacagc
     2641 caattaaaaa ctggtgagtt gctggcgaaa aacttgaatt ttcacagcga attcagatta
     2701 tcttttagta caagagcaat catacgatct aacttatcct gaacttccaa cggtaatctg
     2761 ccgaattcat atgagaacgt acgtcccttt actcgttttc gagcaaattt atctttacta
     2821 tcaaatttcc ataacaaagt tacaaccgct ttatccttta ctcctgaatc aagcaatgaa
     2881 gacatttcct tggttataag ccgcataata ctgtttttaa cctcgtcttc agaaagattg
     2941 tcatcagcag taataagctc aatactcggc gagatattct tcacaacttc atcaatagat
     3001 atatcaccca gcctcagtcg ttcacttaat tcagccagtt gcctgtaatc ggcgtatgtc
     3061 agctcagact gaacaggaaa aagagacaca aaatctttag gtacggacgc tgcttggagg
     3121 gcacgagtca ccttagctgc cgacaatccc tctctttccg caatttgctt ctggttcata
     3181 ccggcctctt tgagacgtac taaacgcaac ccaatttcac gaatattatg ttcaagtgac
     3241 gtctgcaaat ctttcgccag atgctgagct tcagaaacag taagctcatc atccgttaca
     3301 agtacacgta atccaacctt gcatagcaaa gctgctgccc gacgacggga accatccaaa
     3361 atctcaataa gatcgccagt ccgaatccca atacatggat agaattgttg taaatgtata
     3421 gttctggtga tgtcttttag tgagtccggc gtcagggctg tctgttcgcg gccattcacc
     3481 tcttcaacca caaaagtttg agtgtccact ttgtcatgat caaccttgat ctcagtaaat
     3541 tttgcctttc ggccagtatt gagggtaaat acatgaactg gagccgatat ttcttcagta
     3601 ttattcagga tatttgtatt cagcgtacgg ccaatagtcg gtcggtgctt tctgttttcc
     3661 atcagtagtt ggcccttata aattcaatac gatcaaaaac tgctttagcg aaatcttctg
     3721 ctgccatacg tgcatttttc aaagcttcac cactgccaac ataagtaaca ggattcgcag
     3781 atatcacggt atcaaacgac tcgccgcttc tctcaaaacc atcaagacgt ggcattgaaa
     3841 catcaagcat gtcaccaccg aaaatctctt ttgtcaggct atggcagtat ttatgatctg
     3901 acttattcgc cagtttcgac atgaagccaa tatttgcctg caaacggcat gaacatccat
     3961 cctgttcaat aatctgtaca agttcaggta aacgagccaa atatttaagc gtggaatgga
     4021 aatcaacttg tgctggcggc accggtgtaa acatgatatc tgcggcagca atggcatttt
     4081 tcaaaaatgc gtccagatgc gggcctgtat caataaggat gaagtcgaaa tcatgcttca
     4141 gcttatcaat aatattctca cgcaatatcg cgtgtttgtt ttgtcctaat agatgctcct
     4201 cgcacagagt atcccagttt gatgcaataa aggcgtcgtc aatagaagcc ggcataacat
     4261 aaacgccagg gataactgac ggaacgataa aatcttccag tagttcctca cgactcacgt
     4321 tctgcaacat agcctgcggt gcggttgtat caaccagccc caccgcatgc aagtagttca
     4381 gaaacatagt ggctgatgac tggggatcta aatcaaggag cagaatacgt aaatcttctg
     4441 acagcaggtg aggatgagca cgcagggcgt gagctacgga aacactactc acggttttag
     4501 acacaccgcc ttttagacta ccgatgaata tggaatatgc ctcactataa cgatcgcgat
     4561 atttaggaat gccacgatgt gcatagatat cgataatgtt ctggatggtt aatgcatatt
     4621 ttgtcgcggt tcctgctggg cgtttttcaa actgatagcc ctgagcctcc atctcaccga
     4681 ctgcaccatc cacgcttgcg cgcgttaatt ttggaagctt agctacagca gactttgaat
     4741 agacttgata aaaaacatca gcctgaaact cctgcttttg ctcctgaatc tgttttgtta
     4801 gggcgagcaa cattttatct gcacgctgcg caaccttact taactgctca aaactagtca
     4861 ttccccctcc gtgctatgta ggattttaac ttattacaaa aaaaactgca ttagcagggt
     4921 aatgcatgca agtacatatt gtacatgctt atattaaaaa tgcgcgcatt gctgatttat
     4981 taatcatcgg aatgaatatt actgtaagat agtatgcctt tccgggagaa agacgctgat
     5041 ttcccccgaa taatccagtt caccaccaca ctccggacaa cagctttctt caggctccag
     5101 atggtgtatt tcgcgggaaa catgttccgg taacggacgg cgatgacgtg actgtcgcaa
     5161 caggcggggg tagtgcttcc aatcagtaga acatgtgctt ttcaatgaag gagccgatga
     5221 ctttttcgtg gatttccacg gaacgcgaga agcagattgt tttacgtgcc agccgcttga
     5281 tgcgggtgcg gagggtccgg ttgttacgtt caatgcgttg cgtgaagatt ttgccggtca
     5341 ggtgtttctc cttcggcact tctctggcgt agctgcccca gtcatcgctg gtcaccatgc
     5401 caccatgccg atacagaacg gagtcaacag ggctaacagt tcccgacagg tctcatcatt
     5461 ccgtggcccg aaagtcgaaa
//
LOCUS       DAJTVJ010000208         5478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000208 DAJTVJ010000000
VERSION     DAJTVJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.209"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(279..650)
                     /locus_tag="OO448_004106"
     CDS             complement(279..650)
                     /locus_tag="OO448_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339157.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(711..1208)
                     /locus_tag="OO448_004107"
     CDS             complement(711..1208)
                     /locus_tag="OO448_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339158.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1284..2072)
                     /gene="aadA1"
                     /locus_tag="OO448_004108"
     CDS             complement(1284..2072)
                     /gene="aadA1"
                     /locus_tag="OO448_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6339159.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2130..2654)
                     /gene="sat2"
                     /locus_tag="OO448_004109"
     CDS             complement(2130..2654)
                     /gene="sat2"
                     /locus_tag="OO448_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6339160.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(2749..3222)
                     /locus_tag="OO448_004110"
     CDS             complement(2749..3222)
                     /locus_tag="OO448_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6339161.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3554..4090
                     /locus_tag="OO448_004111"
     CDS             3554..4090
                     /locus_tag="OO448_004111"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6339162.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            4205..4531
                     /locus_tag="OO448_004112"
     CDS             4205..4531
                     /locus_tag="OO448_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6339163.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(4719..4958)
                     /locus_tag="OO448_004113"
     CDS             complement(4719..4958)
                     /locus_tag="OO448_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6339164.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1426 a         1306 c         1219 g         1527 t
ORIGIN      
        1 tctctctaga tagcagggat ttcttggcct tgcctcatgc tgatagcttg taaatatcac
       61 acgatcctta gccccacaaa gtgagcatac tccgtagctt tcatttatag aaatcgtcgt
      121 tagattgtcg cagcatgtgc acgttccgac tatttgctca ggaagcatcg ccataccttg
      181 gcctaggtaa agaagatcag actcgtagcc acatcggtgg caactgagct tgtacgcaat
      241 gcccatctgg ttgtctcacc tgaatcgtaa cgtcagagct attcatccat ccattcggca
      301 tcaatgtatg ccactaagcc gaccagctga ccgtttggac tgaagtatgt tgcctgcacc
      361 ccacttgaca tctcatcaat accaccgcac tgcgctaggc gccggtccca cttgcccaca
      421 gaagcgtcag gatcggctga gttccccaag acccttgtga gccatgcctc ctttaactca
      481 acctctcgaa tgattgcgtc ggccagctcc tccgcgttat ctagttcgac atagtctgga
      541 attcggacgt acgttctgaa cacagctgga tacttacttg ggcgattgtc atacatgaca
      601 tcaacaatgt acccgtttgt gtaaccgtct cttggaggtt cgtatgacac tagtggttcc
      661 cctcagcttg cgactagatg ttgaggccta acattttatt agagagcagg ctagttgctt
      721 agatacatga tcttcaggcc gttatctgtc agggcaagcg aaaattggcc atttatgacg
      781 accaatgccc cgcagaagct cccatctttg ccgccataga cgccgcgccc cccttttggg
      841 gtgtagaaca tccttttgcc agatgtggaa aagaagttcg ttgtcccatt gttggcaatg
      901 acgtagtagc cggcgaaagt gcgagaccca tttgcgctat atataagcct acgatttccg
      961 ttgcgactat tgtcgtaatt ggatgaacta ttatcgtagt tgctctcaga gttgtcgtaa
     1021 tttgatggac tattgtcgta attgcttatg gagttgtcgt agttgcttgg agaaatgtcg
     1081 tagttggatg gggagtagtc atagggaaga cgagcttcat ccactaaaac aattggcagg
     1141 tcagcaagtg cctgccccga tgccatcgca agtacgaggc ttagaaccac cttcaacaga
     1201 tcgcgcatag tcttccccag ctctctaacg cttgagttaa gccgcgccgc gaagcggcgt
     1261 cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc gcctttcacg
     1321 tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttgtccaaga
     1381 taagcctgcc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     1441 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
     1501 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     1561 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     1621 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     1681 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
     1741 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
     1801 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
     1861 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
     1921 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
     1981 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
     2041 gtcgatactt cggcgatcac cgcttccctc atgatgttta actcctgaat taagccgcgc
     2101 cgcgaagcgg tgtcggcttg aatgaattgt taggcgtcat cctgtgctcc cgagaaccag
     2161 taccagtaca tcgctgtttc gttcgagact tgaggtctag ttttatacgt gaacaggtca
     2221 atgccgccga gagtaaagcc acattttgcg tacaaattgc aggcaggtac attgttcgtt
     2281 tgtgtctcta atcgtatgcc aaggagctgt ctgcttagtg cccacttttt cgcaaattcg
     2341 atgagactgt gcgcgactcc tttgcctcgg tgcgtgtgcg acacaacaat gtgttcgata
     2401 gaggctagat cgttccatgt tgagttgagt tcaatcttcc cgacaagctc ttggtcgatg
     2461 aatgcgccat agcaagcaga gtcttcatca gagtcatcat ccgagatgta atccttccgg
     2521 taggggctca cacttctggt agatagttca aagccttggt cggataggtg cacatcgaac
     2581 acttcacgaa caatgaaatg gttctcagca tccaatgttt ccgccacctg ctcagggatc
     2641 accgaaatct tcatatgacg cctaacgcct ggcacagcgg atcgcaaacc tggcgcggct
     2701 tttggtacaa aaggcgtgac aggtttgcga atccgttgct gccacttgtt aacccttttg
     2761 ccagatttgg taactataat ttatgttaga ggcgaagtct tgggtaaaaa ctggcctaaa
     2821 attgctgggg atttcaggaa agtaaacatc accttccggc tcgatgtcta ttgtagatat
     2881 atgtagtgta tctacttgat cgatcaggct tttgtatatc tccccaccac ctgaaacaat
     2941 gacatgatcc gttattttct ttaggttggt taaagcatct ttaattgatg gaaagatcaa
     3001 tacgttctca ttgtcagatg taaaacttga acgtgttacg accgcatact ttcggttggg
     3061 taatgctccc attgattcaa aagtcttgcg tccaaccaac agccattggt tataggtaat
     3121 agctttaaac aggagctgtt cacctttggc actccatgga atatcagggc cattcccgat
     3181 aactccattc ttcgatatag ctaccattag tgatagtttc acaattcttc ctcagaggtt
     3241 aacattttat tagtaagcat gctcgttttg tcacccgctg atgcttaccg ttaattaata
     3301 taatcacttg tattaaatag actttcttta aaaatacaag acactctgtt attacaaatc
     3361 gtgcatgccg tctatcctgc ttgcacgatg cacattatct cacccaaaga actcataaca
     3421 ttttaattta tttagcatta aattaaaact ctaccggtaa acaagcatct ctaggcgtaa
     3481 aaaacaggca cttttttaaa tcccagctag aataggctgt ataggcagac agttgcaaga
     3541 caaggagcgt tttatgtcta acagtccatt tttaaattct atacgcacgg atatgcgaca
     3601 aaaaggttat gcgctgaaaa ctgaaaaaac ttacctgcac tggattaagc gttttattct
     3661 gtttcacaaa aaacgtcatc ctcagaccat gggcagtgaa gaggtcaggc tgtttttatc
     3721 cagcttagca aacagcagac atgtagccat aaacacgcag aaaatcgctt taaatgccct
     3781 agcttttttg tacaacaggt ttttacaaca gccgttgggc gatattgatt atatccctgc
     3841 aagcaagcct agacggctac cctctgttat ctctgcaaat gaagtgcaac gcattttgca
     3901 ggttatggat actcgcaacc aagttatttt tacgctgctg tatggtgcag gtttgcgcat
     3961 taatgaatgc ttgcgtttgc gggttaaaga ttttgatttt gataatggct gcatcactgt
     4021 gcatgacggt aagggtggga aaagcagaaa cagcctactg cccacgcgcc taatcccagc
     4081 aataaaataa ctcattgagc aagcgcggct tattcagcaa gacgacaact tacaaggcgt
     4141 agggccatcg ctgccttttg ctttagatca caaataccct tctgcttatc gacaagcggc
     4201 gtggatgttt gtctttccct ccagcacgct ctgcaaccac ccgtataacg gcaaattatg
     4261 ccgccatcat ctgcatgact ccgttgcgcg aaaggcattg aaggcagccg tacaaaaagc
     4321 aggcatcgtt agcaagcgtg tcacttgtca tacatttcgt cactcgtttg ctacgcatct
     4381 attacaagcg gggcgtgata ttcgcactgt gcaagaactc ttagggcata acgatgttaa
     4441 gaccacgcaa atctatacgc atgtgttggg tcagcatttt gccggcacca ccagtcctgc
     4501 ggatggactg atgctactta tcaatcagta aaagtagata aatatcttta tgtagccctg
     4561 agtagcccct gatacttttt atggtgaaaa agataaacaa tcatagaggg gataaatatg
     4621 agccagttct accattgttc agataagttt aagcaagata ctgtcaatca cagtgaccat
     4681 tcatggtcat tacacttcct ccagttgcca gacaatgcct aacataaaaa cgcaaaagcc
     4741 caaaagtcag agcttaggtg ttctaggcgt tttctaagcg ctgaagaaat acgtcttacc
     4801 gtagattgcg tgcgtttctg aagcacattt tccttataca tggctttatc ccaggcagct
     4861 tcatcaagct cttggagtaa caagccagta ataatgcgac actctcgggt ttgtagcgag
     4921 ccgctggtga gatcggagtt atagcggaaa ggggtcattt gtaattattt gtgggtggag
     4981 gatagttaac ttattattgt agttggaaga ggctgctttc caaacattag cacctcgtaa
     5041 tgcttgttac tgtatattaa tttatgctct tagcgcatgt taatgggcta ttacaatgca
     5101 taatgtccac atcaagataa ctgtggctta acacctgtac aagcgaaaaa aatcaatcac
     5161 ctgtccggaa atacgggatc actgtaaagt gttggtgagg cagtctagga gggggattta
     5221 atttgggata ctgatgaaag gattatgatc agttggatat aggatcgtac taagctgagc
     5281 ggcaggttag ctagattagt taaccagata agtgaaatct agttccaaac tattttgtca
     5341 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     5401 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     5461 gcccacaagg gctgccga
//
LOCUS       DAJTVJ010000209         5434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000209 DAJTVJ010000000
VERSION     DAJTVJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.210"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..513)
                     /locus_tag="OO448_004114"
     CDS             complement(<1..513)
                     /locus_tag="OO448_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6339165.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="OO448_004115"
     CDS             complement(759..1211)
                     /locus_tag="OO448_004115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6339166.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OO448_004116"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OO448_004116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6339167.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OO448_004117"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OO448_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6339168.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="OO448_004118"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="OO448_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OO448_004119"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OO448_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6339169.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
BASE COUNT         1286 a         1425 c         1418 g         1305 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctt
//
LOCUS       DAJTVJ010000210         5330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000210 DAJTVJ010000000
VERSION     DAJTVJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.211"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..188)
                     /locus_tag="OO448_004120"
                     /pseudo
     CDS             complement(1..188)
                     /locus_tag="OO448_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
     gene            complement(422..2131)
                     /locus_tag="OO448_004121"
     CDS             complement(422..2131)
                     /locus_tag="OO448_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6339170.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2563..3282)
                     /locus_tag="OO448_004122"
     CDS             complement(2563..3282)
                     /locus_tag="OO448_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6339171.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPNYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3407..3970)
                     /locus_tag="OO448_004123"
                     /pseudo
     CDS             complement(3407..3970)
                     /locus_tag="OO448_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3954..4212
                     /locus_tag="OO448_004124"
                     /pseudo
     CDS             3954..4212
                     /locus_tag="OO448_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4321..4899)
                     /locus_tag="OO448_004125"
     CDS             complement(4321..4899)
                     /locus_tag="OO448_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339172.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
BASE COUNT         1552 a         1119 c          911 g         1748 t
ORIGIN      
        1 gtacttagca ttcttggtat atgatactca agctgaaaca caaagtttat aattctatcc
       61 agactaacag gatcaatgtt tttgttataa caaaactctc ggaatccttg atctttactc
      121 tttcttataa aatcaataag atgcaaccca attccaagcc tcatatcttt agtattaaat
      181 attggaacat actcaccctc tggatgtgac ctttatccat gaagaagatt tggccacacc
      241 atggaaacct gaacaatcat cagtttacgc ccatgtaaac ccctacggga tatttgaact
      301 cgatatggaa ataaggttgc taatcgaggt ggttgcctga aagttggcag ttacccggtc
      361 actacacctt gttggagtga acatatcaaa aacaggtaat tacacaattt aaattacagt
      421 ctcataaaaa atgattaaat ctatttatta tttcaataaa cgtttttttc atcttctttg
      481 aaaattgttc tgctatagta tccggttcca gattgttctc ttttattatt ctacaatact
      541 ctaatattgt ttctttgtct ctatttacta cagatacaaa tacaccagga ataccatctt
      601 tattagttgc ggacagcatt tcgaccaatc gtcttgtggt aaaactatag ttttttagaa
      661 tattacaaaa ggcacgtatt gtttcggtat ggccattata aaatgccata aaaagtcctg
      721 atgtaccgtt gggatttttt atccataata attcatctat atattcctgg tgagggggga
      781 gtatttttag tatatctcca tacgccataa tggcctttgt gtgcccattt tgaagtgcca
      841 taaacaaccc atgctttact ttcaataatt ctttaatttg ttcactacta agcattttct
      901 tattaagcag acttccgaac atacgaatac tatctgcatg tccattctgc aatgcaaaaa
      961 ataacccagg taaatcatgg ctgtctttcg cagctaatag atataatctc ttatcggaag
     1021 ttaacggggt tttaaagact atttccccat aggcttttat tgtgtctaca tgtccgttca
     1081 taaaagacat gcataaaccc ggcacattgt ttgcccctac cgcagcaagc aatgtaagta
     1141 ttgtttcttg actaagattg cacatattta atatcttacc atatacttga ataatgtctg
     1201 aacatccgtt ttgaagtgct atataaaatg caggagtacc atcagagctt ctcgtggcca
     1261 gaaggttcac cagtttagat tgagaaattt caccattttt gtatttttcc atcagtataa
     1321 ataatacttc tgtcagattc tttgttatcg catgatatat aactctggaa ttgatcaatt
     1381 catctggtaa aagtttcact gtgtttgtta gaggggaatt atgcctatct tcaaatatta
     1441 cctgcccggg ttgctccaga ccataaattt tccaataaaa atcatcaaaa aaatccttag
     1501 cggataattg actcagttcg aaatggctat cacaagtaaa tacagtacgt ttatgagtaa
     1561 cagttcgatt aggatcgaaa aactgaacaa cccatttttc actttcttgt acgcgcttta
     1621 ttttcaaccc taacgccatt tggtggttta atgtgcatgc tgataatgtt ctgacaaagt
     1681 ctccattttt tttcatgtta taaaaaaaat tagtaataac tgttccccac tttttatttt
     1741 ccacaaaata aatataagct ggtgattttg aaaaatcttc cgttttttcc aaacatacat
     1801 tttttgcaat agcatcaggg gtgctaagaa gctgatagtt taccttccca tttgtttcca
     1861 taaaattcat ttcccaatag gaagctaggt gacggcaaac tatttgtctt ccatatatat
     1921 cttttgccac tccatttata tttaccttca catttctctt ttctgaaaaa taaggtgctg
     1981 ttttattctt aaactgaatt agttcactgg agtttgagac cctcataata gaacttgtta
     2041 ttgttctatt atctctgtta acagacatat ctgttgataa cgaatttttt gttgaaaata
     2101 tactagatga aaatgtttta tttaacggca tagaagacaa ccatagagat gttataatac
     2161 tattagattg cagttactaa tagtaaaagc ctccttacaa ggaggtagct tattcagaat
     2221 gctcctataa gatagcgagc tcgtcaacga tacagatgat ctgattatat tgtgcaaaag
     2281 gagaacagga aaataggcac tggaaaagat gaaggcctga tgggagcatt aactaatgca
     2341 aaatccgaca ggttaatatc ccgaaaagca atcaaacgac tcaaacagga gatgcacgct
     2401 gaagtggtgg acaactgttc ggatttcaga tgagtagctc agcagtttat gagccgaatg
     2461 ctctgtcacg ggcaggaatc aggctgcata cgcagtgcca aatctaatcg tagttattct
     2521 aaccagtttt caagctacaa ggtggtgtag ctggcatctt ctctactcta tcatcaaacg
     2581 ataaaatggt tcctctcgta acatttgctc ttgcctttca cttccatctc gatcgcttcg
     2641 tagttcatta cgatagctga catatttcca gtcttcaggt ctaacatctg acgccggata
     2701 ctccccggga gcgattttgc ttcttaatag attagactcg agacacatta taaattgtcg
     2761 tattttatga agtaataaag cactatattg cgagcattcc tgatctgatt ttacatatag
     2821 cgtaaactga gctcctatcc ccacacgaga ttgctgagaa actcgattca tatcagttat
     2881 cttccattta tctataggac tgtcctctga aaataataaa ccagacaaaa tttggaatgc
     2941 cagaggaact tgctccctag ctatactaat atgaaacttg tccccaacaa aatccccctt
     3001 actctgagga ttttcacgac atgcatgaat aaacacatct ccactatggt tcacaatctg
     3061 gaatccttgg tacatgacag aaaaaaattg acatatatca gagtaatttg gcacgttcga
     3121 tgctactttg aaatagtacg ctgggtattg ccgtatttgg ttgtacaata tattgaagtt
     3181 atttttcaat ctttcattcg cagaaagcat ctgcggtatt tcagacctca caacattcaa
     3241 agaatctaaa tttagtttta gacagggctt ttttatgggc atagaaaacg tcctctataa
     3301 aatagatata ttatcttata ttgattgtct atcatctttt ataaaacgat acattatatg
     3361 taatgtatgt aagcgtaagg gtcttctccg atcatggtgg gaaggcttag aacgccatat
     3421 tcagtttccc atagtggaac atcactccca gcttaaagcg ctcccggttg cggtagccct
     3481 tggcttttat tctcagcagc ctgatcttgc tgttcagtgc ttccgcattt ccgtttgata
     3541 cgccatggcg catggcgttc aggataccgt acaacctttt ccctatcgtt ttagccacat
     3601 tcttcatcat ggggacgtca ctgttggccg ccagcgacag ccatctctgc cagtcgttgc
     3661 gtctttcctc gctccatggt ctgctccaga tatcctttgc cagttctttc agcgcccagc
     3721 acaggctcgt cagcttcatc tgcgcacgca gccatatcag cttttcctgc ctggattcgg
     3781 tcatccattt attgctgtgc tgccacagga agcgagttcc ctttgcctga cgacgacttt
     3841 caacaggaag atgcggatgt tcattctggc gggttttatc caccacctca cccggttgtt
     3901 tcgccacatg aaagcgatcg aaggctattt tctcaaccgc attgggtaag tggatgcgcg
     3961 ctgctcttag tttgctgagc cagtttagca ttgccttcta catgaccgat gcctgagccg
     4021 gtataccgaa ctttattgtc ctcaaccagt catgttataa gcaagaaatg cactcagcgg
     4081 atagagcgac ataatctgaa cctgcggaca catcttaagc ggcttacccg caaaaccata
     4141 tgcttctcaa agtctgatga tatgcactac aagatcatcg gctggtatct gacaatcaac
     4201 caccaccact aaatctgcgt cacgacctgc aactgagtct ggttgaacag gctgaggccc
     4261 tgctgaagca acgcgaacct ctcgactctt gttggatttt accaaatagt ttttctaaaa
     4321 ttaaacacat ttatataagt tctttgctga acaaaacgca gcccgccatc ctttttcatt
     4381 cataaattca gcaatggcag ataaaacttt ttcatcaagc atataaaaaa taatgccagt
     4441 agactcaata tgcctcatac aaataaggcc gcacatatcc gtaatatatt tgtcaatgct
     4501 gctataattt gcatggtaat atgccatgct attatctgat gattttacat atgcagtatg
     4561 acatattttt ccttcacgat taacaaaact gacaaaacca ctttcaccag gagtaatata
     4621 taaagatgaa tttaacgtgc gctcactaat attcattaat tgttgaaatt caatggaata
     4681 gttttcagtt aatgtatcta ttatcttggc tttgtctttc tttgttatct cattgtcttt
     4741 ttcggcaaga aaaattgcca ctttatgaca tgcattcagc tcgttaattt tacctgataa
     4801 aactgctttt cttaccacat cgttgaactc atcgatagtg tgttttttat tatatatatc
     4861 atgaaaaata tagcctaaag acttaaacga gactttcatt ttattttata ttctccttaa
     4921 atattaacag gatatatata tcacattttc ctctatattt tcagaaacca gctatcattt
     4981 tccccattac tggtacgtcg cttcagatat ttccgcagca agcgataacc ctgcgcacta
     5041 tgaggatgcc attcattata atgtttgaac accttcgcga gactacttta ctgctgttaa
     5101 cacgtcaggt tcgaacataa cactgattta atcatatttc atcgttttga cgaagttttc
     5161 tgccattcca ttttgcaccg ccgtgtgttt gggggcaagg ccgaccatct gtgtttcatt
     5221 cgccagatat aagtgatgag atggcattct ccctccttca cggcagaagc attttgaccg
     5281 tttaggggat gcggacgatt ttcatgcctg ctaagatact ttcgttactg
//
LOCUS       DAJTVJ010000211         5321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000211 DAJTVJ010000000
VERSION     DAJTVJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.212"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(31..867)
                     /gene="kduI"
                     /locus_tag="OO448_004126"
     CDS             complement(31..867)
                     /gene="kduI"
                     /locus_tag="OO448_004126"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6339173.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1154..2335)
                     /locus_tag="OO448_004127"
     CDS             complement(1154..2335)
                     /locus_tag="OO448_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6339174.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            2590..3819
                     /locus_tag="OO448_004128"
     CDS             2590..3819
                     /locus_tag="OO448_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6339175.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            4279..4911
                     /locus_tag="OO448_004129"
     CDS             4279..4911
                     /locus_tag="OO448_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6339176.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            5245..>5321
                     /locus_tag="OO448_004130"
     CDS             5245..>5321
                     /locus_tag="OO448_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6339177.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADL"
BASE COUNT         1503 a         1215 c         1100 g         1503 t
ORIGIN      
        1 gcaaatcttt aacggccact atgccgacaa ctagcgcaaa tctttaacgg ccacatggtc
       61 catatcatca aagacctggt tttcaccaac catgccccag ataaaggtat aagctttggt
      121 tccgacgccg gaatggatcg accagctggg ggagatcacc gcctgctcgt tatgcatcac
      181 aatgtgacgc gtttcttgcg gctgccccat catgtggaaa acgcaggcgt catcatccat
      241 attgaaatag aaataaactt ccatccggcg ctcgtgagtg tgacacggca tggtgttcca
      301 caagttacct ggagccagct ccgtcagccc catactcaat tggcaggttt ccagcacatc
      361 cgggacaaaa tatttgttaa ttgtgcgacg gttactggtg aggttatcgc ctaacgtgac
      421 tggagatact tcgtccggtg tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca
      481 attgtaataa aactttgccg gagtggcggt atcaatactg gcaaagacaa cttcttttgc
      541 acctttacca acatacaggg cgtcgcggtg accgatttca tagcattggc catcgacagt
      601 aatcgtaccg gcaccgccaa tattgataac acctaactcg cgacgttcaa ggaaatagct
      661 tacgccgagt tgtttaccaa cttccccgcc aacggaaacc gttttagtta tcggcataat
      721 gccgccaaca ataattcggt caatgtggct gtaaaccatg gtgtactcat cggcgacaaa
      781 taccttttca accaaaaatt cattgcgcag cccttgggta tccagcgttt ttgcgtgcgc
      841 actgtggatg ctctgtctta cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat
      901 agaaaacgat agtgaaacac tgtttcgttt tctctataaa gggatgatat gcccatgggc
      961 tagggttttc aattaaaaat aaaacaatgt ttcattttta agttaaggat taaaaaaagt
     1021 gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg agggagccca gaaaatgtat
     1081 gtcgacttgc cagccatcat tattcaacag attaatgacg gcagatattt atcggtaagg
     1141 aggaaatgca gagctactcg tcacgttcaa tggtcaatgc cacgccctgg cccccgccga
     1201 tacaaagcgt tgccagccct ttgcgggcat tacgtttcac catttcatga accagagaaa
     1261 ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga
     1321 cattgacccg acgctcatcc cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa
     1381 acgcttcatt agcctcgata agatcgacat cagccaactg ccagcctaca cgctccaggc
     1441 aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg
     1501 caaatgcgcg gatgcgggcc agcacgggta aattcaacgc tcgtgctttg gcttcgctca
     1561 tcatcattac cgcagctgcg ccatcgttta tggatgatgc attccccgcg gtcacagaac
     1621 cgagactatc aaatgaagga tttaaacggg ctaaaccttc tgcactggcg tcagtgcgtg
     1681 gctgttcatc ggtatcaaca accaacgtct gaccgttact ttggatcatt accgggacga
     1741 tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg
     1801 cgtaagcatc ctgcaactga cggctgatgc catattcgcg agccagattt tcggcggtga
     1861 caccaatatg ataatcattg aaggcatccc acaacccatc atgcaccaga ctgtcaacca
     1921 actggctatt gccaagctgt gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc
     1981 tcatgttttc ctggccaccg gcgatgacaa tatcagcctc gccacactgt atcgcctgag
     2041 tagccagatg cagtgcttta agtccggaac cgcaaacgtc attaatcgtg attgcagaaa
     2101 cgctattagg aagaccacct ttaatagccg attgccttgc cggattctgc cctgcccctg
     2161 cagtcaacac ctgaccaaga attacttcat ccaccgcata tgcaggaacg ccggtacgtt
     2221 ctattaacgc tttcacgacc agactaccaa gttccacggc ggaatgaccc gctaacgcac
     2281 cacgaaagca gccgataggt gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa
     2341 cctcgcacca attaacagtg caatagtaaa tgattgttaa ttacagttat cttaattgtt
     2401 taaaaaaagt gatttttatc acaaaggaaa tatgcctgag cagtagtcag agtcataact
     2461 ggcacgtaag gtttgcaacc actaacccac caatagatgg gtagataagg cgttaatctc
     2521 ccatacttaa cctggtttat ggtaaattgc cctccatttt gtttaatttg tagatgatac
     2581 gttcagataa tgtctaatat ttggtctaaa gaagaaactc tgtggagttt cgcgctctac
     2641 ggcacagccg ttggtgcagg cacgctcttc cttcctattc agttaggttc ggcaggggct
     2701 gtggtcctgt ttattactgc tctggtcgcc tggcctttaa catattggcc acataaagcc
     2761 ttatgccagt tcatcctctc atcaaaaaca tcagcatgtg aagggataac gggcgcggta
     2821 acacactact atggcaagaa gattggtaat ctgattacca cgctgtactt catcgccttt
     2881 tttgtcgtcg tgttgatata tgcagtggca attaccaact cacttacgga acagctggca
     2941 aagcatatgg ttattgatct tcgcatccgt atgttggtga gtctgggtgt tgtattaatt
     3001 ctgaatctca tttttctgat gggacgtcat gccactattc gggtaatggg atttttggta
     3061 ttcccattga ttgcctattt cttatttctt tccatttacc ttgtcggtag ttggcaacct
     3121 gatctattaa cgacccaggt agagttcaat cagaataccc ttcaccagat atggatatcg
     3181 attcccgtga tggttttcgc ctttagccat acgcccatta tttctacgtt tgccatagac
     3241 agacgtgaaa aatatggcga acacgctatg gataaatgca aaaaaattat gaaagtcgct
     3301 tatctcatca tctgcataag tgtactgttc tttgtcttta gctgcctgct ttctattcca
     3361 ccttcgtata ttgaagcggc taaagaagaa ggggtcacca ttttatcggc gctttctatg
     3421 ctgccgaacg ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt agttgcgatg
     3481 tcgaaatcat tcctgggtac gtactttggc gttattgaag gtgccacgga ggtcgtcaaa
     3541 acaacactac agcaggttgg tgtaaagaaa agtcgtgcat ttaaccgcgc actatcaatt
     3601 atgttggtat cgctgattac cttcattgtt tgttgcatta acccgaacgc gatttcgatg
     3661 atttatgcga tcagcggccc gctcattgcc atgatacttt tcatcatgcc tacgttgtca
     3721 acgtatctga tcccggcgct taaaccctgg cgctccatcg gaaatctgat tacgctgatc
     3781 gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat atccgttgtg gatggttcaa
     3841 aattgctaca attttgaacc attccatttt tcataatatt catttgttat tattccgccc
     3901 tatcacttct ctcgtcatat cctagaatta tcagttagtt aaacacacaa agagatctga
     3961 acatcttaaa taagactatt taagatgcat aacttagatt cgcaagatat cttctggtca
     4021 ttcaaaaaca atttgcgata tagatattct cataccatga gatagttatc tggaccacgt
     4081 tcgaccatgc aagattaaat cagtgaaaca taatcatatt taataacgag aaatgcattt
     4141 ttaaatgtct taatcagaat tatagcaaat acagattaat cgcaattttt cccagcaaaa
     4201 ttacaaaatc aagatgtatt gacatcatca atcaccaatg aaattatgca attatatacg
     4261 gatagggagg ttcttaacat ggggctttgt agtcgttata aaagtcttac atgcaatagt
     4321 tgctcaatgc attgccaaat aatgccagaa gagtcaccgc gtttgcagta ttgcgcaaac
     4381 tcgtgttttt gtatgtggcc cgaagaaagt tcatatttta atcgtggtgt ggtggaaggt
     4441 atcttaacaa aaaaccacaa tgcgagatta agcggatata tttttgtgga tttttcagta
     4501 agttttttac gtctattcct tgaaaaagac tggattgact atcttgcaag tactgacatg
     4561 gggatagttt tagtcagcga ccgcaatatg caatcgttag ctaactactg gcgaaaacac
     4621 aacaccgcaa tatctgctgt catatataat gatgatggtc ttgatgttgc caatgaaaag
     4681 atcagacaac tatttattgg tcgttattta tcatttacca gaggtaacac attaactcag
     4741 atggaattca ccataatggg gtatatggtt tctggttata atccatatca gattgctgaa
     4801 gtgctggata tggatatccg tagcatctac gcgtacaagc aacgaatcga aaagagaatg
     4861 ggtggtaaaa taaacgaatt atttattcgt tcacattcgg tccaacattg atactcaacg
     4921 accagccaga atcatactct gttcatacgg gcatctattg aaatgcccgt attttataaa
     4981 taagcagtaa ccccaagagt aaacaacata gtatttaaaa ccatcacacg aaaaattcag
     5041 aagcattacg aaatatgaat ttacaaacaa tagtggcata aatgttaacc atgttaattt
     5101 acgtaaagtt ttacgttgca acattaaagc ctcatttcaa tcatcatgat aaatataaaa
     5161 ttaatatata tttatgccgt aaataccaat atacttagca aactatgtga tctccatttc
     5221 gattgattta gtgtttattg acgtatgtac tggattatta acgataatat cgagttctgg
     5281 cctgagcacc gaaaattaat atcggtacat aacgccgatc t
//
LOCUS       DAJTVJ010000212         5274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000212 DAJTVJ010000000
VERSION     DAJTVJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.213"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..287
                     /locus_tag="OO448_004131"
     CDS             <1..287
                     /locus_tag="OO448_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6339178.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
     gene            289..816
                     /locus_tag="OO448_004132"
     CDS             289..816
                     /locus_tag="OO448_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6339179.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(845..1378)
                     /locus_tag="OO448_004133"
     CDS             complement(845..1378)
                     /locus_tag="OO448_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6339180.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1381..2271)
                     /locus_tag="OO448_004134"
                     /pseudo
     CDS             complement(1381..2271)
                     /locus_tag="OO448_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2370..2552
                     /locus_tag="OO448_004135"
     CDS             2370..2552
                     /locus_tag="OO448_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6339181.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            2598..2696
                     /locus_tag="OO448_004136"
                     /pseudo
     CDS             2598..2696
                     /locus_tag="OO448_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(2751..3419)
                     /locus_tag="OO448_004137"
     CDS             complement(2751..3419)
                     /locus_tag="OO448_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6339182.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSA
                     SGKK"
     gene            3476..3745
                     /locus_tag="OO448_004138"
                     /pseudo
     CDS             3476..3745
                     /locus_tag="OO448_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            3860..4030
                     /locus_tag="OO448_004139"
     CDS             3860..4030
                     /locus_tag="OO448_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339183.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(4157..4714)
                     /locus_tag="OO448_004140"
     CDS             complement(4157..4714)
                     /locus_tag="OO448_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6339184.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(4711..4986)
                     /locus_tag="OO448_004141"
     CDS             complement(4711..4986)
                     /locus_tag="OO448_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6339185.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            5171..5274
                     /locus_tag="OO448_004142"
                     /pseudo
     CDS             5171..5274
                     /locus_tag="OO448_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT         1470 a         1124 c         1208 g         1472 t
ORIGIN      
        1 ttgcgctggg aagtggtgcg caagttgcgt ctagtggcga tatttatggt tcagtgtggg
       61 aaaacaactg gttaagcaca tggctgcata atcatgtcgt tcgggatatt cgtcttggca
      121 gcattgaata taaaaacgta tggcgcgact acggctttgg cgatgcgtca ggttatgttt
      181 taacagccgc aattaacagc aatgcggatg atattgtcga cactgttgcc agaaggccaa
      241 ttcagaaatt gattggggga atatggtaca acgtggggag tgtttaagat gatgcacctg
      301 aaaaatatta aggccgggaa tccgaaaaca ccggaacaat accagctaac taaaaaagcg
      361 ggcgtggtat ggctcttttg cgaagatggc aaaaactggt atgaggaaca aaagaatttt
      421 cagccagaca ccataaaaat tgcttacgat gagaacaaca tcatcgttgc tgtcagtaaa
      481 gacgtttcaa ctattaatcc tgaagggcta agcatagttg aagttcctga tattacggca
      541 aatcgtcacg cagacaataa cggaaaatgg atgtttctgg atggtaaagt agtaaagagg
      601 gaatatacag aacaggaact gcaacaacag gccgaatcac aaaaggccgc attgctttcc
      661 gaagctgaat cagtcatcca gccgctggaa cgagctgtca ggctgaatat ggcaacagac
      721 gaggaacgca cacgactgga agcatgggaa cgctacagtg ttctggtcag ccgtgtggat
      781 acggcaaatc ctgaatggcc acaaaagcct gaataaaaat taaggcccga tagcgggcct
      841 tctctcattc tggttgttcg ggaaacgtta ctggcaggct ggaggtgtct gtggattcaa
      901 ccttctgcgc atagagcatc cactcggtta atttttgttt attctcatcg gaaatgatgc
      961 ccagctgtag ctgagagtcc cacagctggg ttttatccct gacgagctgc agcaggctct
     1021 gcttttcatt ttccgcttgt tgcctttgct cttcctcggt ataagttcgc tttatcaccg
     1081 caccatcttt gaacatccag ttgcccgaaa tatcagcccg gcgatttgct gtaatatcag
     1141 gcaactcaac gacacttaaa ccttctggat taattgctga tacatccttg tctatagcaa
     1201 taattattcc ctcaactgtg taagccagtt ttattgtatc ctcctgaaag ttttttaatt
     1261 cctcatgcca attttttcca tcttcggacc ataaccagat aacatcaaaa ttctttgtta
     1321 gctggtattg ttcaacggtt tttggattac ctgcgacaat gttttttaaa tgctgcatga
     1381 ttatacctgc gatacgttat accatgttcc gttaattaac ttttgtattg gtctgaatac
     1441 tgccggatca tcaccatcga cttcaccgat aataccaagc cccgtgatta catggcctga
     1501 tttttcatac atcacgcctt tcttcatggt ctaaacaact cgtgtgccaa gtctgacatc
     1561 tctcacatag cgggaatcaa agttaccgta atccgaggga ttaacacgtc ccgtaatatt
     1621 tatggtttta ttactttgaa tgcttccgga gacaaagcgc ataacatgga cgttattagc
     1681 ataaacatcc agattaccat cgcctttttg tttaaagccc gtgtcattat cacccaatac
     1741 aatcgaatta ccgccaagag cactggatgt tccgataccc agtgcaccat tcagttgacc
     1801 accagataac ggcagtgcac caacatctcc tgctgaaggt tttctggtgg tgtaaaattc
     1861 ggaccagtcg gcttcaaaac cataaccatc acgggctgaa cgataaaaaa taccgccatt
     1921 tttataatta atccgaaact gagctgcagg acaacttcct tctcccatat aaaaatgaat
     1981 aattaacgtt gatgcaccac taatagttgc gttataggct ccgctactcc agttccatcc
     2041 aactgcttta tcattcgcaa cggtgcttcc tgttttccct aaggcaaacg caccaacatg
     2101 acttgctttt aatgtgatat cggaggaacc atcaaaagcc acattgctta ttttcctggc
     2161 agtttttaat tttgcagctg tagaagcatt gccggatagt tcaccagaaa ggccaccgct
     2221 gaatgtttgt cgattagtcc aggtattcgc tgtactgagt aacggtattt tctccccgct
     2281 tgtgccgagt tctcgtaaac cgaggtttta gataatggcg gtttctggcc tgcatggcat
     2341 gatttgtgct tttggacggg agattcagcg tgctgattgg ctatgtaagg gtatcaacaa
     2401 atgaccagaa tacagacctg caacgaaacg ctcttgtttg tgcaggatat gaacaaatat
     2461 ttgaagataa attaagcgga acaaggacag gccgacctgg attaaaacgt gctttaaagc
     2521 gccttcaaaa aggtgacgca ctggttgtct gaaaactgga ctgactgggg cactgtatgc
     2581 ggcatcttgt cgtgctggtg gtggaagcct agaaaaagta tcgggtgttg ctgaaccgtg
     2641 ttgatacatc aactgcacct gatattgagt ggcctacgaa ccctgtcagg gagtaatcat
     2701 tgggattatg ccgcagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     2761 cctgaagcgg aaaacattac tacagtacct tgaaccctgg ttttaacatt ctcgaaatgc
     2821 tctgagagta tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc
     2881 tgattgtaaa tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga
     2941 attgtggctc cgtatagagt tccatcgtca gttaaggcct gcgccgcatt gcgtattaca
     3001 cagctttttg tagatatatt tccaggcagg cagtgaagaa ggtaaaacat ggaaatggaa
     3061 tcaaattgac catgtaacgc cgcgggataa ggttcaaaaa catcatggct aattttatgt
     3121 ttaatttttg attccccagc ccttgtagat gccgcgttca ggctagcttc gttcaaatcc
     3181 attaaagata tcagactact ctcaggtacg tgagtaaggt aaaacccagt tccaacacca
     3241 atatccagat ggttgttacc taaatgttcc agaaagtgtg gaagaaggtg ttcctttgta
     3301 ggacatcccc atgcaagccg atttgatact cccaaaaccc accagtcata aagctttagg
     3361 gtaagtggtg tgtaaatttt agccccatca tctgtgtttg ttttcattga tttcaccatg
     3421 ttatagtttt atttgtgaat taaatcaatt atggcgatga attacaaggg gttaaatgct
     3481 gccgcagcat agcgatattg aaatagccta gtatgcttcg atacagcagg agccgaatgg
     3541 ctggaagacc gtcaccacac agttctacat ccaggaattt agtgagtata ttgcgccact
     3601 gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa agatcgttgc tggaggcatg
     3661 gaataaatat cgggtattgt tgaatcgtgt tgatacatca actgcacctg atattgagtg
     3721 gcctgcaaat cctgtcaggg agtaatcatt gggattatgc cgcagacacg tcgtatgcag
     3781 gaacgtgctg cggttagttt gtgagctttc gatagtggtt gttatttttg cccttatttg
     3841 ttccggaggc catggttcaa tggtccgtct gccccctgtg gtgatgtcag caaaatcagc
     3901 cactgcgcga accacaatag cccgggaaga tgctgaagat caccaggtaa agctgtcagc
     3961 gcagaaactg gaagaactgc tcgcatcaat ggttaaggat gaggttgatc gcaatgatgg
     4021 gatttattga cgtcagcgag agcagaagga agaactgaat aacctgaatg atttacgctc
     4081 aatcagagcg atggcgatta gcggcaatca cgctcgataa aattaacctc ggtgatcatc
     4141 cggggttttg cattcattaa aaccgcatca acctttccac caattgttct ttacgggcaa
     4201 cgatccaccc atgttgctcc agataaaatt taaaccgttc cagagtgcat accatcgcat
     4261 cggcggggac tttttctgtg aactcgacct gaccgtgttt atcgaagtgg atcagtaatg
     4321 cgcatccatt atttggggcg ggggagtttt gtgctgcagg aggacgtttg tggagttctt
     4381 cttccattgc gttaaatttt tcaatgtaag cttcttttag tctggctgct tttttgccag
     4441 taaatcccat tacgaggaac atgaagccgt cttttgtgat caggtagcat cgtgattcac
     4501 gttctgcgcc gttgccaatt tcgacagtct gaacatcggc cgaaaagttg gctgatgtaa
     4561 attcgggaga gcagtcgagt gtgtcaatct tagctaaaac gtgtttatgt tgtttatcga
     4621 agtagtcggc aacagcttga gaggtggtaa ccaggcgacc atctttgaaa aagacttttg
     4681 gtgactcgtt agaaaaagtt atagctgtat tcatatgtga tgactccaat taattggatg
     4741 atcaccacct ctgacgccaa tcagggaggt ggtgagacgt acagggttgg cgtaaccggt
     4801 catcacaaaa cccggcgcgg atttctccgc ccctgcacgc cccaccataa tgcgaatgtg
     4861 gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc gttgtgatgt tatccgagac
     4921 gccaatcccg acaaccgatt ttgcggttgt gtgaggaata tagccccgga gaacaaaaac
     4981 tgtcaacggg caaaaaaatt tccgttatca ggaatgtata aaattaaagc gatcgagcaa
     5041 aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat caggcagtta aaaacacgga
     5101 aaacggtata aaaaagcgcc tcaaaatcct gttttttctt tcagatacaa tgtcttattg
     5161 cattttttaa aacatgtcag tgtggtacat ggatatcgat accatcgcca attataaact
     5221 gggcggtgca cagcaacatg atagcgtacg cctcgatccg tgggtgttta tgtt
//
LOCUS       DAJTVJ010000213         5271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000213 DAJTVJ010000000
VERSION     DAJTVJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.214"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..471)
                     /locus_tag="OO448_004143"
     CDS             complement(<1..471)
                     /locus_tag="OO448_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6339186.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="OO448_004144"
     CDS             complement(471..1391)
                     /locus_tag="OO448_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6339187.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /gene="pgrR"
                     /locus_tag="OO448_004145"
     CDS             1529..2428
                     /gene="pgrR"
                     /locus_tag="OO448_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6339188.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2765..4378
                     /gene="mppA"
                     /locus_tag="OO448_004146"
     CDS             2765..4378
                     /gene="mppA"
                     /locus_tag="OO448_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6339189.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4429..>5271)
                     /gene="ynaI"
                     /locus_tag="OO448_004147"
     CDS             complement(4429..>5271)
                     /gene="ynaI"
                     /locus_tag="OO448_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6339190.1"
                     /translation="VIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQQIK
                     KGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS
                     NFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSS
                     ISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQF
                     ADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPP
                     EQGR"
BASE COUNT         1423 a         1203 c         1226 g         1419 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac
     1441 tatagatatt gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt
     2641 aatatattga tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt
     2701 tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa
     2761 acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct
     2821 ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg
     2881 ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat
     2941 tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt
     3001 ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg
     3061 cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg
     3121 gcagcgtctg gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg
     3181 aatcaacaac gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac
     3241 cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa
     3301 tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga
     3361 gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt
     3421 caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact
     3481 gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc
     3541 gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga
     3601 tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac
     3661 gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg
     3721 ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc
     3781 agatgttacc gccggattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga
     3841 actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct
     3901 gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc
     3961 atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac
     4021 ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga
     4081 ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc
     4141 acgctttaac aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt
     4201 taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat
     4261 tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc
     4321 cattaataac ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg
     4381 tgatgggaac tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg
     4441 ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc
     4501 gtgtgactgc acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc
     4561 agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa
     4621 ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat
     4681 ctcacgtacg gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat
     4741 ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa
     4801 cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca
     4861 gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat
     4921 actgaaagga cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt
     4981 accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct
     5041 catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat
     5101 gcgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa
     5161 ctgattaatc agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat
     5221 aaaatcgata acgtctgggg tgataaattt gagtttatag ttttctataa c
//
LOCUS       DAJTVJ010000214         5219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000214 DAJTVJ010000000
VERSION     DAJTVJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.215"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(291..575)
                     /locus_tag="OO448_004148"
     CDS             complement(291..575)
                     /locus_tag="OO448_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339191.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="OO448_004149"
     CDS             complement(593..790)
                     /locus_tag="OO448_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339192.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESKWEEHSH"
     gene            1002..1298
                     /locus_tag="OO448_004150"
     CDS             1002..1298
                     /locus_tag="OO448_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339193.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="OO448_004151"
     CDS             complement(1843..2076)
                     /locus_tag="OO448_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6339194.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="OO448_004152"
     CDS             complement(2083..2613)
                     /locus_tag="OO448_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6339195.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2591..3349)
                     /locus_tag="OO448_004153"
                     /pseudo
     CDS             complement(2591..3349)
                     /locus_tag="OO448_004153"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="OO448_004154"
     CDS             complement(3291..3614)
                     /locus_tag="OO448_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6339196.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="OO448_004155"
     CDS             3679..3870
                     /locus_tag="OO448_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6339197.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT         1366 a         1433 c         1119 g         1301 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccatttactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgcatata taaacactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgttc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgcacccg gtaaatttta accacatgca tagctatgca gccatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagca ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct catagaatgc tcctttcagt ctgatattct
     2641 tcccgccgtt cgggtctgca atgctgagac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagggagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacttttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattcccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca agcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttcc agtaaccggg catgttcatc atcagtaacc
     3601 cgtatcgtga gcatcctctc gcgtttcatc ggtatcatta cccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 atcagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcacgtcagc gggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcgcc atcagtgcgg atggtatgaa aagtgcgcca taccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca gccgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ttaccgttta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catggggata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtggcagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAJTVJ010000215         5167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000215 DAJTVJ010000000
VERSION     DAJTVJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.216"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1310..1519
                     /locus_tag="OO448_004156"
     CDS             1310..1519
                     /locus_tag="OO448_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339198.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1585..2118)
                     /locus_tag="OO448_004157"
     CDS             complement(1585..2118)
                     /locus_tag="OO448_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339199.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2121..3047)
                     /locus_tag="OO448_004158"
     CDS             complement(2121..3047)
                     /locus_tag="OO448_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6339200.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            3224..3622
                     /locus_tag="OO448_004159"
     CDS             3224..3622
                     /locus_tag="OO448_004159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339201.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3826..4740)
                     /locus_tag="OO448_004160"
     CDS             complement(3826..4740)
                     /locus_tag="OO448_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6339202.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1436 a         1351 c         1104 g         1276 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc
      781 atacagctaa ttacatataa atactattaa accatctgga cattaaaaat aaactcttat
      841 aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat
      901 cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag
      961 ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag
     1021 aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac
     1081 ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact
     1141 tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc
     1201 tccttacata tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa
     1261 tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt
     1321 aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc
     1381 atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag ctcttactga
     1441 tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt atcgaccaaa
     1501 gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaattcttt cgcgggtcgt
     1561 gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga caggtttctc
     1621 caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt
     1681 cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac
     1741 tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat
     1801 attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc
     1861 gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac
     1921 gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga
     1981 aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag caacagcttt
     2041 ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg taagtaattt
     2101 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
     2161 cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
     2221 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc
     2281 cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga atagtccggt
     2341 tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagcttca
     2401 aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca
     2461 atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt
     2521 tttcgcaatt cccgcaactg ctctttttgc tgagtgagag tcaggcttcc ggaatccttt
     2581 ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta
     2641 cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgccg
     2701 ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg ttcgccagct
     2761 tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggt
     2821 aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg gtcagccgcc
     2881 tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg tgcggaatat
     2941 tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca
     3001 gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc
     3061 tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat
     3121 tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac
     3181 gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca tcagcaacat
     3241 cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca
     3301 gagacagatt attgacctga tcgacaacga agccggactc acagaacagg aacgcaaact
     3361 tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga gcgaacgaaa
     3421 acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca atcaccggct
     3481 ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgagct
     3541 atgctataaa aaagacagga tattacaagc acttaaggaa cttaagtacg atctggagac
     3601 atccccaaac ccagacgcgt aagtgcctcc atccgtgttc cgattccctg gtgcttcctc
     3661 gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccac cacaatcacg
     3721 cggaaagccg cgccattgca gggctgaacg gggaaaaacg gcaaaaaacc tgctgctcaa
     3781 aaagacgcca gaaaggcgct gttatctgat ataggcttat ttggtctact ctacgagacc
     3841 tcgttttttc agttggtaat accaggcgcg actgatccct aatgcctccc acggtttcag
     3901 cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta gaaccgccct tgcgaccgcg
     3961 tgcagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag tttccggcgt
     4021 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt cagccagtga
     4081 aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg cttcaagcca
     4141 ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc gcgtcatttc
     4201 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac gctctgaggc
     4261 gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc gccactccat
     4321 caccagccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt tcacatccgc
     4381 acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg ccttaaccga
     4441 ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag cgtgtccgtt
     4501 cacaggattt ttaacggtga tgttcggcgc gggagcgttc cggtcgctcc agtcaatcgc
     4561 agccccctcg cggtcaacgt caaaaaccag ccagaactgc gcatgaggct ggttctgctg
     4621 tatgtaccgc gcaagaagcg cacgcccttt gccggagatg cgaagaccaa aagcgagatc
     4681 atccgtgtga tagggacgat ggggtaaatt ttcgtcgaag tattgaagcg cggcgctcat
     4741 gtgttacgat tctctccggg ttatgcgacc tacacgccct ctgtatttca tgttgaatct
     4801 tgccggatct ggacatgatt tcaggggcgt ttgtttttca gtcgcttaca ctacacccaa
     4861 cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt taccataact cccgttgtac
     4921 gcccccctta aaacgccctc agtcgctttc tggcgcaaaa aacgcaaaaa gacaagaaaa
     4981 caccaggcca aaccagaaaa acgcgtcaga atcgttttca gggcgttgca taactatgca
     5041 cgaaagcgat gtgcgatcgg tttcgtgcgt tctgaacacc gaacggctca aaacgacaag
     5101 ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg tttccaaact tcacatctcg
     5161 ccaggac
//
LOCUS       DAJTVJ010000216         5114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000216 DAJTVJ010000000
VERSION     DAJTVJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.217"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..379
                     /locus_tag="OO448_004161"
     CDS             <1..379
                     /locus_tag="OO448_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339203.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OO448_004162"
     CDS             complement(575..2125)
                     /locus_tag="OO448_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6339204.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OO448_004163"
     CDS             2297..2743
                     /locus_tag="OO448_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR6339205.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OO448_004164"
     CDS             complement(2937..3872)
                     /locus_tag="OO448_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6339206.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OO448_004165"
     CDS             4384..>5114
                     /locus_tag="OO448_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339207.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAJTVJ010000217         5029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000217 DAJTVJ010000000
VERSION     DAJTVJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.218"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            72..187
                     /gene="rrf"
                     /locus_tag="OO448_004166"
     rRNA            72..187
                     /gene="rrf"
                     /locus_tag="OO448_004166"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(305..748)
                     /locus_tag="OO448_004167"
     CDS             complement(305..748)
                     /locus_tag="OO448_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6339208.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            905..1834
                     /gene="metA"
                     /locus_tag="OO448_004168"
     CDS             905..1834
                     /gene="metA"
                     /locus_tag="OO448_004168"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6339209.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2103..3704
                     /gene="aceB"
                     /locus_tag="OO448_004169"
                     /pseudo
     CDS             2103..3704
                     /gene="aceB"
                     /locus_tag="OO448_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3734..5025
                     /gene="aceA"
                     /locus_tag="OO448_004170"
                     /pseudo
     CDS             3734..5025
                     /gene="aceA"
                     /locus_tag="OO448_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1260 a         1317 c         1283 g         1169 t
ORIGIN      
        1 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
       61 aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg ccgaactcag
      121 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
      181 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc attggcgcag
      241 aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc ctgggcaggc acggcagggg
      301 cagcttattc ctccacatac gccagattca gcaacggata cggtttcccc aaatcgtcca
      361 cctcagagcg tcccgtaacc ttaaaaccca ccttcttata gaacccaacc gcctgctcat
      421 tttgctcatt aacgttggtt gtcagttccg gtgccatcga gagcgcatgc tccaccagca
      481 tccgacccac gccgcagccg cacacatcag gatcgataaa cagcgcatcc atatgctgcc
      541 cacttaacaa cataaaccca accggctgat cccgttcatt aaccgcgacc cacaacggcg
      601 cttccggcag gaaggaacga actagcacct ccagctcggc ccgatactct gctgataaaa
      661 aatcgtgtgt ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc ccttcctcat
      721 gccgtgagcg gcgaatacta ataaccattt tctctccttt tcgtcattct tatattctaa
      781 cgtagccttt tccttgaaac tttctcacct tcaacatgca ggctcgacat tggcaaattt
      841 tctggttatc ttcagctatc tggatgtcta aacgtataag cgtatgtagt gaggtaatca
      901 ggttatgccg attcgtgtgc cggacgagct acccgccgtc aatttcttgc gtgaagaaaa
      961 cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa attcgtccac ttaaggttct
     1021 gatccttaac ctgatgccga agaagattga aactgaaaat cagtttctgc gcctgctttc
     1081 aaactcacct ttgcaggtcg atattcagct gttgcgcatc gattcccgtg aatcgcgcaa
     1141 cacgcccgca gagcatctga acaacttcta ctgtaacttt gaagatattc aggaacaaaa
     1201 ctttgacggt ctgatagtta ccggcgcgcc gctgggcctg gtggagttta atgatgtcgc
     1261 ttactggccg cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca cctcgacgct
     1321 gtttgtctgc tgggcggtac aggccgcgct caatatcctc tacggcattc ctaagcaaac
     1381 tcgcaccgac aaactctctg gcgtttacga gcatcatatt ctccatcctc atgcgcttct
     1441 gacgcgtggc tttgatgatt cattcctggc accgcattcg cgctatgctg actttccggc
     1501 agcgttgatt cgtgattaca ccgatctgga aattctggca gagacggaag aaggggatgc
     1561 atatctgttt gccagtaaag ataagcgcat tgcctttgtg acgggccatc ccgaatatga
     1621 tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa gccggactag acccggatgt
     1681 accgtataac tatttcccgc acaatgatcc gcaaaacaaa ccgcgcgcga gctggcgtag
     1741 tcacggtaat ttgctgttta ccaactggct caactattac gtctaccaga tcacgccata
     1801 cgatctacgg cacatgaatc caacgctgga ttaatcttct gtgatagtcg atcgttaagc
     1861 gattcagcac cttacctcag gcaccttcgg gtgccttttt tatttccgaa acgcacctca
     1921 gcaagtgaat aaattttatt catattgtta tcaataagtt atcaagtatt tttaattaaa
     1981 atggaaattg tttttgattt tgcattttaa atgagtagtc ttagttgtgc tgaacgaaaa
     2041 gcgcacaacg atccttcgtt cacagtgggg aagttttcgg atccatgacg aggagctgca
     2101 cgatgactga acaggcaaca acaaccgatg aactggcttt cacaaggccg tatggcgagc
     2161 aggagaagca aattcttact gccgaagcgg tagaatttct gactgagctg gtgacgcatt
     2221 ttacgccaca acgcaataaa cttctggcag cgcgcattca gcagcagcaa gatattgata
     2281 acggaacgtt gcctgatttt atttcggaaa cagcttccat tcgcgatgct gattggaaaa
     2341 ttcgcgggat tcctgcggac ttagaagacc gccgcgtaga gataactggc ccggtagagc
     2401 gcaagatggt gatcaacgcg ctcaacgcca atgtgaaagt ctttatggcc gatttcgaag
     2461 attcactggc cccggactgg aacaaagtga tcgacgggca aattaacctg cgtgatgcag
     2521 ttaacggcac catcagctat accaatgaag caggcaaaat ttatcagctc aagcccaatc
     2581 cagcggtttt gatttgtcgg gtacgcggtc tacacttgcc ggaaaaacat gtcacctggc
     2641 gtggtgaggc aatccccggt agcctgtttg attttgcgct ctatttcttc cacaactatc
     2701 aggcactgtt ggcaaagggc agcggtccct atttctatct gccgaaaacc cagtcctggc
     2761 aggaagcggc ctggtggagc gaagtcttca gctatgcaga agatcgcttt aatctgccgc
     2821 gcggcgccat caaggcgacg ttgctgattg aaacgctgcc cgccgtgttc cagatggacg
     2881 aaatccttca cgcgctgcgc gaccatattg ttggtctgaa ctgcggtcgc tgggattaca
     2941 tcttcagcta tatcaaaacg ttgaaaaact atcccgatcg tgtcctgcca gacagacagg
     3001 ccgtgacgat ggataaacca ttcctgaatg cttactcacg cctgctgatt aaaacctgcc
     3061 ataaacgcgg tgcttttgcg atgggcggca tggcagcgtt tattccgagc aaagatgaag
     3121 agcgtaataa ccaggtgctc aacaaagtaa aagcggataa agcgctggaa gtcaataacg
     3181 gtcacgatgg cacatggatc gctcacccag gccttgcgga cacggcaatg gcggtattca
     3241 acgacattct cggctcccgt aaaaatcagc ttgaagtatt gcgcgaacaa gacgcgccga
     3301 ttactgccga tcagctgctg gcaccttgtg acggtgaacg caccgaagaa ggtatgcgcg
     3361 ccaacattcg cgtggctgtg cagtacatcg aagcgtggat ctccggcaac ggctgcgtgc
     3421 cgatttatgg cctgatggaa gatgcggcga cggctgaaat ttcccgtacc tcgatctggc
     3481 agtggatcca tcatcaaaaa acgttgagca atggcaaacc ggtgaccaaa gccttgttcc
     3541 gctagatgct gggcgaagag atgaaagtca ttgccagcga actgggcgaa gaacgtttct
     3601 cccagggccg ttttgacgat gccgcacgct tgatggaaca gatcaccact tccgatgagt
     3661 taattgattt cctgaccctg ccaggctacc gcctgttagc gtaaaccacc acataactat
     3721 ggagcatctg cacatgaaaa cccgtacaca acaaattgaa gaattacaga aagagtggac
     3781 tcaaccgcgt tgggaaggca ttactcgccc atacagtgcg gaagatgtgg tgaaattacg
     3841 cggttcagtc aatcctgaat gcacgctggc gcaactgggc gcagcgaaaa tgtggcgtct
     3901 gctgcacggt gagtcgaaaa aaggctacat caacagcctc ggtgcactga ctggcggtca
     3961 ggcgctgcaa caggcgaaag cgggtattga agcagtctat ctgtcgggat ggcaggtagc
     4021 ggcggacgct aacctggcgg ccagcatgta tccggatcag tcgctctatc cggcaaactc
     4081 ggtgccagct gtggtggagc ggatcaacaa caccttccgt cgtgccgatc agatccaatg
     4141 gtccgcgggc attgagccgg gcgatccgcg ctatgtcgat tacttcctgc cgatcgttgc
     4201 cgatgcggaa gccggttttg gcggtgtcct gaatgccttt gaactgatga aagcgatgat
     4261 tgaagccggt gcagcggcag ttcacttcga agatcagctg gcgtcagtga agaaatgcgg
     4321 tcacatgggc ggcaaagttt tggtgccaac tcaagaagct attcagaaac tggtcgcggc
     4381 gcgtctggca gctgacgtga cgggcgttcc aaccctactg gttgctcgta ccgatgccga
     4441 tgcagcggat ctgatcacct ccgattgcga cccgtatgac agcgaattta ttaccggcga
     4501 gcgtaccagt gaaggcttct tccgtactca tgcgggcatt gagcaagcga tcagccgtgg
     4561 cctggcgtat gcgccatatg ctgacctggt ctggtgtgaa acctccacgc cggatctgga
     4621 actggcgcgt cgctttgcac aagctatcca cgcgaaatat ccgggcaaac tgctggctta
     4681 taactgctcg ccgtcgttca actggcagaa aaacctcgac gacaaaacta ttgccagctt
     4741 ccagcagcag ctgtcggata tgggctacaa gttccagttc atcaccctgg caggtatcca
     4801 cagcatgtgg ttcaacatgt ttgacctggc aaacgcctat gcctagggcg agggtatgaa
     4861 gcactacgtt gagaaagtgc agcagccgga atttgccgct ataccttcgt atctcaccag
     4921 caggaagtgg gtacaggtta cttcgataaa gtgacgacta ttattcaggg cggcacgtct
     4981 tcagtcaccg cgctgaccgg ctccactgaa gaatcgcagt tctaagcaa
//
LOCUS       DAJTVJ010000218         4935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000218 DAJTVJ010000000
VERSION     DAJTVJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.219"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..1226
                     /locus_tag="OO448_004171"
     CDS             63..1226
                     /locus_tag="OO448_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6339210.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1686..2123)
                     /locus_tag="OO448_004172"
     CDS             complement(1686..2123)
                     /locus_tag="OO448_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339211.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2212..>4935)
                     /locus_tag="OO448_004173"
     CDS             complement(2212..>4935)
                     /locus_tag="OO448_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCR6339212.1"
                     /translation="RRKYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRK
                     TMTWSRYGQLLTVTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDT
                     QGHEMRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIR
                     LTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEAD
                     RLTHRTVKGETAERWRYDERGWLTDISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQ
                     TEALLWQHETRHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDR
                     LHRETLRSFGRYELTIAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSY
                     SYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHY
                     LYRYDHHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYL
                     YDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPG
                     SFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILAD
                     RVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWC
                     AEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGL
                     KGGWNFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGN
                     NGNNMQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRD
                     GFLTQSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT         1091 a         1236 c         1269 g         1339 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gatgattttt ctttaatatt
     1321 cattatgtta ataagttctg gatgagtgtg ctctcattgg attttttatc cgtcttgttc
     1381 tgccgcagga tgagactata catcgtcgag atcgcaatga tgatatgagt tgttcctcct
     1441 gcgacggctc taatcaaaaa agagcaggag tgcttttcca tctattgcga tgatggcttt
     1501 attatctgaa ggatgacagt cacatttaaa aaatatttaa tctatagatc acaatgtagg
     1561 gtcatggaat atatgtatac aactattttc ttcttcactt ttaattatcg tattcattat
     1621 aaaactaagt taaaaaaact cctagagagt gagttattaa tatattttta aataaaatta
     1681 agttatcatt catcgatgca ttttatagaa tcatcaatgg ccataatagt actattaact
     1741 ctcaatttta tatttttatc atcactacaa atattaactg aatttatatc aatgattttt
     1801 ttattcttat cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga
     1861 tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag
     1921 gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat
     1981 tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag
     2041 gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc
     2101 cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca
     2161 ctcgcagaaa tagagaaaaa aatacaatta ttaataactt tgccatattt tttagtccgt
     2221 aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt
     2281 agatgaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc
     2341 gtttaaacaa ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg
     2401 tactaaacgt ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct
     2461 ccatacccct tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt
     2521 acattgcata ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga
     2581 gttatcatca ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa
     2641 acaggtcgct aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa
     2701 attccatccc cccttcagtc caatcggatc ctgagtgata tatcgcccct gcagcgggtc
     2761 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
     2821 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
     2881 gtattctgcg caccattctg ttgccccttc cgtgctgata agcaccagcg gcaggccgcg
     2941 atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt ccatctggtt
     3001 ttgcatctgc tccaccgtca ggccgcacga tgccagccag cggcggcttt cctcactcac
     3061 ccggtcagcc aggatttcac tttccagccg gtcgagcatc tgcaccagca ccggcgggaa
     3121 caccacactg ccaccgtctt cgccgccgga ctgctgaagg gcatccgcca ggctgcggcg
     3181 ctgcgttttc gccagctcac cggtggcagt ttcgaccctg atgagtggcg tgaagctccc
     3241 cggctgataa atcgtctgga tgcgggttct gtcgttctgt attgtggtca gccggtcgcc
     3301 gtcccagccg taccaggtca cttgcggttt ccgtgacagc gacatccagc ccgtcaggtc
     3361 ccgttcacgc cgccataccc gttttgccac cctgcggccc agcgggtcgt aaagatagcg
     3421 actttcgacc agcggctctg catattgtgt ccgcgtgtag tgcaccagcc ggtgctgact
     3481 gtcgtaatgg taccggtggg tgcgctcatc atccgtgcgg ataacccctt ccgggatgag
     3541 gtcggttttc tctgtcagtc tgccgtgatg gtcataccgg taaagatagt gcgcgtcacg
     3601 ggcgatacgg ttatccggcc acatgctgag ggtgctgtcc gggtgcagct ccgggtcggg
     3661 caggcggttc cccgccgggt ctgtggcata cgggatgcgg atatccagat tcgctgcggt
     3721 ggtgtgaacg ccggtcagcc tgccggtggt gctgtagctg taactccggg tctggcgcgg
     3781 gctgctgatg cggatgagtt cgccgttgtc gttccaggtg taatcgcggt cagacagcag
     3841 gctgttcagg tgctggctct gtaactgccc ggcaggggta taagcgatgg tgagttcata
     3901 acggccgaag ctgcgcagcg tttcccggtg caggcggtcg cgggtgtact ccaccagcgg
     3961 tgtgtcgccg agtttcatgc ctgacagcca gccgctgccg taggtcagcc attccacggc
     4021 ggacaggctg tccggtatac agcggttcgc cagcccctgc gcgttgtacg catgtctggt
     4081 ctcatgctgc cagagcagtg cttccgtctg cgggtgatgc accgtctgac gctcaccggt
     4141 cagccggcct ttctcatcat acccgtaatg caccgtcacc cggtgccctt cgctgagatg
     4201 gctgatgtct gtcagccagc cgcgttcgtc gtaccgccac cgctctgcgg tttcaccctt
     4261 cacggtgcgg tgcgtgaggc ggtctgcttc gtcatagtgc cagtgggtga ccagcccctc
     4321 atcctcgctg cggataagtt tgccggtcag gtcgtggtga taacgctgtg tgcggccgtc
     4381 aaagccggtt tcctgtatca gccggtcgag tacatcgtaa cggaaggtgg tgtggctgcc
     4441 gttttcactg gtcagacgga tgacccgtcc ggcagcatcg tattccatac tgcgcgtcag
     4501 cccgccctgc gtggtacaga tggcttttcc ccacgcatcg tactgtgtcc cgtttctgct
     4561 gccgtccggg gcaatgacgg cggtcaggtc accggcggcg ttgtattcat accgcatttc
     4621 atggccctgc gtgtctttca ccgcaattaa ctgtccacgg ctgtcgtatg cgcggtactg
     4681 gctcagccct tcctcgcggt gcaccgccgt cacctgacca aaacggtcat ggtcataacg
     4741 ggttacataa ccggaacagt cagtgacggt cagcaactga ccgtaacggc tccatgtcat
     4801 ggttttccgg ctgccggtgg catcttccgt tgcgcagggt aagtcactgt gtggattatc
     4861 ataacggtag cgggtgatat cgccgtcagg ggcggtttcc tgaatcagac ggccggattc
     4921 atcatatttg cggcg
//
LOCUS       DAJTVJ010000219         4914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000219 DAJTVJ010000000
VERSION     DAJTVJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.220"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            133..510
                     /locus_tag="OO448_004174"
     CDS             133..510
                     /locus_tag="OO448_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339213.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            533..685
                     /locus_tag="OO448_004175"
                     /pseudo
     CDS             533..685
                     /locus_tag="OO448_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(741..2357)
                     /locus_tag="OO448_004176"
     CDS             complement(741..2357)
                     /locus_tag="OO448_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6339214.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(2358..3689)
                     /locus_tag="OO448_004177"
                     /pseudo
     CDS             complement(2358..3689)
                     /locus_tag="OO448_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
     gene            3759..>4914
                     /locus_tag="OO448_004178"
     CDS             join(3759..4008,4008..>4914)
                     /locus_tag="OO448_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6339215.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT         1340 a         1219 c         1050 g         1305 t
ORIGIN      
        1 aagtttcaaa tatcaatgcc caagatgcca tgccttcagt atggagcctc cggatctacc
       61 ggagatgctt tgggactaat gctccgcttt gcctcgggta tccgttcgac ctatgtttaa
      121 gaggaaacgc acgtgtctgc cgccaaagct cttgcctatg cactttgtga caagttggaa
      181 agcaaaaaag ttgatgaagt cgtacgaaat cttgtttcta gcggctggaa tattagggaa
      241 gtggcctggg aagcttcgtc ctttgatcat gaaatgccct actttgacac ggggcgggac
      301 ttcgatattg gatttcctga cacgctgtca tccaagattg agtttttgac cgcgaatgga
      361 tcaatcgtcc aagcacgtgc tcgcctgcac ttcaagcagg ctctaatttt tgccaaggcg
      421 tccaaacatg tcgccgcgct caagaactca ttggattact actatggatc gtctgtaatc
      481 ccccctcaaa accacggagg ctatttgtag aaaattccat taatatgaac tcaaggtgtt
      541 ccctacggac gaatcagcga tgaaggtggt gtacctggct atccaggcgg cgaccaagaa
      601 atggacgatg ccgatccgca actggaagcc ggccatgaac cgttttatga tcgaatttgg
      661 tgaccgttta aatggccacc tttaactgac cggcaaaagc acttacacaa aagggtttac
      721 aggctctttc agtcattcct ttaatgcgta aattgctctc taaccactct ctcagcccac
      781 ccttccatat cctctctcga tacttgcagc tcccccaacc cctttggatg attcacccct
      841 ctatgcccgt cttgtccata taattgatca aacaaactat ttggccaatt caatgagctc
      901 ttcaccactc cacgccgtat cttttcaaaa tcattccgcc atgtttcgct atcaacgcca
      961 ctcaacactt tggtagaaag cattttcacc ccatccgatg catccacctc tagcaataca
     1021 aattcactgc cattacgttg caccccaacg gctttaatca ctcttggtga gccatcttta
     1081 cacagatgta atcggctacg cccaactttt ggcaaaacca aggtttcttc aaaaagcacc
     1141 cgacatgcaa atttagtttt tagaattgaa agtagctcat caaaagcagc aaatcgatta
     1201 gcaaaaatag agttgtaatt ggttgcatcc tgcttcccac ccacatctgc cgctgctagg
     1261 acaccaccta agtgtggctc atcagtacta accaaattac tattttcact tgttgttgct
     1321 tcttcttttt ctaaaatagt ttggctactt tttcttgccc tgcgagattt ttcaattcga
     1381 ctaggtctac taaaacttat ccatgtcggc tcagcctcta tgactcgtgt gtctgtgtct
     1441 atattggctg tttcctcatc gtccaactgt agatgctcat catcaacaac tgactctgtt
     1501 gaaaccgcta tgtgctgcgt actaccatca cctgcctcct tttcctgaaa agaggcatgg
     1561 ctaatagctg ttgtgctagg aagcatagcg ttgatttcta aacctactat ttcctcaact
     1621 aaataatcct tatcctcgtt agaagaacgt ccttttacat gtaatttcca accctgcatg
     1681 ggcggagggt caaagctaaa gcaccagctt tcaacgccgt tcttaatttc tctattttgt
     1741 tgatagtgcc taaaaatact ttcatacgaa tccataacat cttgatccga aaacaaccaa
     1801 accaaaagct gcactacggc cgactgctct aacgcccctt taggaaacga tgaactgggt
     1861 aagatcctta tctctaaatg atctcgctca acctcatact gaacgtcgaa ctcttgctgt
     1921 aaagcggtac tgctcaaaca gcttcgacag aaataggagt taattaaaaa taatgaccga
     1981 gctagctcta actgtgggat gtgataacga gtcttgctat atggtgtttc agcttcgtaa
     2041 ataaatgaac tctgcgctcc atccttgttg cgtacggaag caaaggacga gagattagga
     2101 aaatcgatta ttttggctcg ttgaagatcc gatgcttcaa actcaatcac acgatccgcc
     2161 ttatttattt gctttgttgt actgttaacc accttagctc gacttaataa aggcaaatga
     2221 gtaaatcgag tccatttccg ttcttgcatt ggattaaacc aaacaaaaat acgccactcc
     2281 ttatgacccg agttacggaa taaatgacca atatgtacaa cctgcacatt gtcattaaat
     2341 gtagctagcc taaccactca ctcttcccca taaaccattt ccaagaatct ttgtgcttcc
     2401 tcagttatcc gctctttaga taacgttgca cttcttaata atcgccagcg cctaagctca
     2461 acatcttcct gtttaagctt aataaaagct tggctaatcc gtctaatttg ataatcttcc
     2521 acactctctg agtaacgctt taagcaaagc gctaccaaag gcagtttctg tagatttttt
     2581 gcaagagagg ttccgttagg agtttgcttt aacagccagc tcgatgtcgc tcttgggtga
     2641 tcaaggctac tatctagacg ctttatgatt cttaatagtt gtcgtacagc aattcgatct
     2701 ctttggttcc aatcaactct aggggcgggt gccagacgct cttgttgatg ctgttgattc
     2761 cagtgaacta gccaatccct gtcatgtcgg taaagccaag cgtatagcac cccaccctct
     2821 aaagactgtc ttgccgcctt aattccttgg tatttatgca ctagttgctg ccagtcttta
     2881 tgcttaacag ataaatcttc agagttaggt tgcacagact ggctaacagg tcttgtcgtt
     2941 atagagtgct cagtaagagc acttgcctgc tgtagcgctt ctataaccgt tagttttggc
     3001 aataaggctt gccacacaat actatgctgt aaataactaa aggcttttct atgcttacgg
     3061 aatatacttt tcagccaaca cgtgtccttg ttctctgcca actttaaatc cagtttctct
     3121 agtgcctcat cactaaaagt ctgcctcact ctctccgcca ccaagtcatg acgaatgtgc
     3181 ttgcttttgg ttagccctag atcctgcgct aagcgctgat aaaacagcgt ccactgctca
     3241 aggcttgggg aaagctcttg cgctcgtgga gcatctaaca gaggggctat ataagcagct
     3301 agtgctgtta attgagatag ggagtctttg gggtagtctg aaagcagctc agtatgaccc
     3361 aaagcccaaa attgatgtcg gtgatcatct acagctctat caaagaagac taaagcaccg
     3421 tgttttggac aatatggcaa agcgggcaaa taccaatctc gttgccaaaa ggcttcccca
     3481 tacctattta gctgaagagc aacgcaatca gggcagtatc taaagcggtt atcgctctta
     3541 actctagaag cagcgactcc tagcattaaa tgcaccgcac cttgcgcttg gtactccatt
     3601 aaccgaatag cttcgtctcg gcgctccttg cctacaaacg gagcatataa agggaataag
     3661 gtatgctcat aaataagctg ctgaactgaa gtggcacact gaatttggcc acctgaacag
     3721 aggtgatatg ctcacctcag aacaacacag gtgctccaat gaaaaaaaga aattttagcg
     3781 cagagtttaa acgcgaatcc gctcaactgg ttgttgacca gaaatacacg gtggcagatg
     3841 ccgccaaagc tatggatgtt ggcctttcca caatgacaag atgggtcaaa caactgcgtg
     3901 atgagcgtca gggcaaaaca ccaaaagcct ctccgataac accagaacaa atcgaaatac
     3961 gtaagctgag gaaaaagcta caacgcattg aaatggagaa tgaaatatta aaaaaggcta
     4021 ccgcgctctt gatgtcagac tccctgaaca gttctcgata atcgggaaac tcagagcgca
     4081 ttatcctgtg gtcacactct gccatgtgtt cggggttcat cgcagcagct acagatactg
     4141 gaaaaaccgt cctgaaaaac cagacggcag acgggctgta ttacgcagtc aggtacttga
     4201 gctacatggc atcagccacg gttcggccgg agcaagaagc atcgccacaa tggcaacccg
     4261 gagaggctac cagatgggac gctggcttgc tggcaggctc atgaaagagc tggggctggt
     4321 cagctgtcag cagccgactc accggtataa acgtggtggt catgaacatg ttgctatccc
     4381 taactacctt gaaaggcagt tcgccgtgac cgagccaaat caggtgtggt gcggtgatgt
     4441 gacctatatc tggacgggta agcgctgggc gtacctcgcc gttgttctcg acctgttcgc
     4501 aagaaaacca gtgggctgtg ccatgtcgtt ctcgccggac agcaggctca ccatgaaagc
     4561 gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg gtgatgttcc acagcgatca
     4621 gggcagtcat tatacgagca ggcagttccg gcagttattg tggcgatacc agatcagaca
     4681 gagtatgagc cggcgcggaa actgctggga taacagccca atggaacgct tcttcaggag
     4741 tctgaagaac gaatggatgc cggtggtggg ttacgtaagc ttcagcgagg cagctcacgc
     4801 cataacggac tatatcgttg gatattacag cgcactaaga ccgcacgaat ataacggtgg
     4861 gttaccccca aacgaatcgg aaaatcgata ctggaaaaac tctaactcgg tggc
//
LOCUS       DAJTVJ010000220         4802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000220 DAJTVJ010000000
VERSION     DAJTVJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.221"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(277..1320)
                     /locus_tag="OO448_004179"
     CDS             complement(277..1320)
                     /locus_tag="OO448_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6339216.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1732..2916)
                     /locus_tag="OO448_004180"
     CDS             complement(1732..2916)
                     /locus_tag="OO448_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6339217.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3216..3310)
                     /locus_tag="OO448_004181"
     tRNA            complement(3216..3310)
                     /locus_tag="OO448_004181"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3274..3276),aa:Sec,seq:tca)
     gene            3603..>4802
                     /locus_tag="OO448_004182"
     CDS             3603..>4802
                     /locus_tag="OO448_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR6339218.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1253 a         1056 c         1090 g         1403 t
ORIGIN      
        1 gataccagtt caaccgaaaa cgccagagga ggggattacc cgctggcagg gtaaatctgt
       61 ggtttttgat gccagctata acgtgtttga cgataaagta ttacgaaaga actttaatga
      121 gttcatggag taattcgtta aagtaattta caacgctatc cttttgcaaa ggaaactttc
      181 agaaaataac cccagggtaa tcacctgggg aaggaacaca gtgttattat tggataataa
      241 attattactt gttttaaagt tacactaatg caatatctac tgatactgga tgcttgaatg
      301 tttcatccct gctgcggaaa agggcaatat ccagggccag atatattcac cgttcatatt
      361 tctgcatttc tgtacaattt cctcagaaag tgtcttgtca attcttttcc acaggtgctc
      421 ctgctgtctt aaaatacttt cgcatatttc gaaatcatct tcctgaatca gcatttcggc
      481 gcatttaatg atttctgatc gttcatgcag cacggaggaa tggttataga gccagtttac
      541 aaacagcaat gcttccggat attgtcccca taatggtgag tatgttgcat agtaatcttc
      601 tgccgtatcc agagagccgg agaagtcatg ccacacagca agtggaaatg gatgcagatt
      661 tccgcacagc atttctgcga tgtaagtgtt tgtggcaaag ccatggcgaa aagctgtggc
      721 ggccttaaca tgttccccgt tcagaaaatg caggagaccg agttcatacc agtaaggtga
      781 aaattcatca gcatgctctt tcagtacact gaaggctcgc tcatgatcgc cggtgcgtaa
      841 cagctctgaa cccaacaacc agcgagcccc ctgattatct tcaggattgt atgccagtat
      901 tttatcagtg agcataaccg catcctgatg gcgctgtaag tgcacatttg cgagaattgc
      961 tgcataaagc gcgcgcagat aaggccggtt ttcgggatgc atccagatta tttcaccaca
     1021 gaaactttcc gggatgatac gattacctgc cgccagtcct ttaagagagg cgttcagtgc
     1081 cttacgcggg gcattctgtt ccagaaatgc atatgccaga tgagcgtgta tatcaataaa
     1141 gtccggctcc cgttgagcaa ggcgtcggag tttgttgatg taactcgtct cgctgatttc
     1201 atcagcgtcg tatgcatcaa gcacttcttc aaactccccc agcaatgggc caaaccaggt
     1261 atccggaaat acgaaataac ccttgttatc gtgcacttca aagcataatc tatcgttcat
     1321 ttctgagcct gtaaaacatg tggtgatata gtctgagagc ataaaagttg agattctgtg
     1381 tatcaagtcc tgtcttggca ccgaatgaat attgcttacg aagccccttc atgctttcct
     1441 tgagaacaat aattttatct ttatgtgaat ctcatttttt ataatgatgt cagatgattg
     1501 aaatgctaaa ttatatctga atcaactttt cctttgctaa aggtatcgaa ataactgcaa
     1561 ggctgtattt tgtaattgcc aatttaaaaa tgtcaatttg tttttaatta tgtttttgta
     1621 ataagtttga agacgcaaaa aaaataatat aataacaaaa atcctccagc cttgtaggcc
     1681 agaggataaa atacttaatt gaggaaaata tcagggaaaa gtatttctga cttaactttc
     1741 ccggctggag agtgaatcaa tgtaatcagc gtaccattgc atcatttcac ggcggccatc
     1801 gagatacagg gcatgattat aggtacccct gatggaattt ttatccacat gagctaactg
     1861 catttcaatc caggcggaat taaagccttg ttcatgcaga attgtactca tggtgtgtct
     1921 gaaaccgtgc cctgtgagtc ttcccttata tccaagtaat tcaattactt ggttaatgct
     1981 ttccttacta attggtttgc ggggatcatt tctaccgata aagactaggg gatgatgttt
     2041 tgagattggt tcaagctgct taaatagcat aatgacctgc tcagagagcg gcacaatatg
     2101 aggtcgcttc atcttcatta cttctgccgg gatctcccac aattttgtct caaaatcgat
     2161 atcttcccag cgtgcaaaac gcagttcttg tgttcgtacg ccggttaaca taatgatctg
     2221 agtagctgtc ttagtgatta tacttccggt ataacccgcc agatcgttga ggaagtgggg
     2281 aagttcatta gcagtaagaa agggaaaatg tacttttttc ggtgtagcta aagcactggc
     2341 aagatcgggg gctgggttat agtctgcccg gccagtaaca attgcatagc ggaacacctc
     2401 accacagcgc tggcggacct tgcgcatttt ctccagagct ccacgttttt ccattttacg
     2461 cagtgcttcc agtaactcca ttggtttgat ttcggcaata gggcgcttgc caatgtaggg
     2521 aaaaatgtct ttttcaaagg tatcaatgat ttcatcacga tagcgtaatg accaccgatc
     2581 agcttttgac tggtgccatt cgcgagcaat agcctcgaac gtatttttca gactcatttg
     2641 ctgcgcgatt ttttcttcct tttttgcctc ccctggatcg cctccagcag cgaggagttt
     2701 cctggcttca ctgcgtttct cgcgagcatc ggctagcgtt acatcggggt atacgccaat
     2761 agaaagcatc ttttctttac cagcaaagcg gtacttcatg cgccagtatt tagaaccatt
     2821 agtgttaatc agtagataca taccgccgcc atcagcaagc ttatagggtt tgtctttggg
     2881 ttttgcggtt ttcactttga tatcggttaa tgccatgaca tcctccggct tttgatgaat
     2941 tcatgttcct gagggggatt tctgccagta actaacctga tacccccaat acaccccttg
     3001 tataacaaag atattgttaa aagccgttgt tatgtatcac atttttccta ttatgggggt
     3061 gcttgtattt caataggaaa taacaccccc taaagtaccc ccaaaagaaa cttgatatgg
     3121 ggtgaagtag gttgacctca ggatacaaat aaatactgat atacaaggga ttagaagaga
     3181 tttattgaca ttgggagagt tcagaagagg taaactggcg gaagatcaca ggagtcgaac
     3241 ctgcccggga ccgctggcgg ccccaactgg atttgaagtc cagccgcctc accggagacg
     3301 acgatcttcc gcgcctcgat tgctacatgg aggcggggcg cattatagct acttccttga
     3361 gtttctacat cccccagatc agttttttcc gcctaataca cgcagcgtat tatcacgaaa
     3421 gcattataac tcatataaaa cccatatttt acaatgatat atgagcggaa ttaacgctac
     3481 cacgatcaca taaaacaaga atcataaatt aataaccaga tatcggaata ttcgctctca
     3541 cagggatggt ttacaaaatg cgttatcggt ctcagcaccc ctatagcatc aaggaaaagc
     3601 agatgaagag tgaagtgttg tccgttaaag agaaaattgg ttatggcatg ggagacgccg
     3661 ccagccacat tattttcgat aacgtaatgt tatatatgat gttcttttat accgatattt
     3721 ttggcattcc tgccggattt gtcggaacca tgtttttggt cgctcgtgca ctggatgcga
     3781 tttccgatcc ttgcatgggg ttgttggccg atcgaacgcg ctctcgctgg ggtaaatttc
     3841 gtccgtgggt actgtttggc gcactgccat tcgggatcgt ctgtgtactg gcctatagca
     3901 cgccagatct cagtatgaac ggcaaaatga tctatgcagc aattacttac accctactta
     3961 ccttacttta taccgtcgtc aatatccctt actgcgcatt gggtggtgta atcaccaatg
     4021 acccgactca gcgtatctcg ctgcaatcct ggcgttttgt gctggcgacc gcgggaggca
     4081 tgctttctac tgttctgatg atgccactgg ttaatttaat tggcggtgat aataaaccac
     4141 tcggtttcca gggcggtatc gcggtccttt ccgtggtggc attcatgatg ctggcatttt
     4201 gtttcttcac cactaaagaa cgcgttgaag caccacctac aacaacgtct atgcgggaag
     4261 atttacgtga tatctggcaa aacgaccagt ggcggattgt cggtttacta accattttca
     4321 atatcctggc ggtgtgcgta cgcggtgggg cgatgatgta ttacgtcaca tggattttgg
     4381 gcacgccgga agtgtttgtc gcttttctca ccacttattg cgtgggtaac ctgattggtt
     4441 ccgcactggc aaaacctctg accgactgga aatgtaaagt cactatcttc tggtggacga
     4501 acgccctgct ggcagtgatt agcctcgcga tgttctttgt tcccatgcag gccagcatca
     4561 ctatgtttgt cttcatcttc gtgattggtg tgttgcatca actggtgaca cctatccagt
     4621 gggtaatgat gtccgatacc gtcgactacg gcgagtggtg caatggtaaa cgcctgaccg
     4681 ggatcagttt tgctggcacg ctgtttgtgc tcaaactggg gttggccttc ggcggcgctc
     4741 ttatcggctg gatgctggct tatggcggat atgatgcggc agaaaaagcg cagaacagcg
     4801 cc
//
LOCUS       DAJTVJ010000221         4799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000221 DAJTVJ010000000
VERSION     DAJTVJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.222"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="OO448_004183"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="OO448_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6339219.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="OO448_004184"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="OO448_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR6339220.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="OO448_004185"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="OO448_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6339221.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OO448_004186"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="OO448_004186"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6339222.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OO448_004187"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="OO448_004187"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6339223.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..>4799
                     /locus_tag="OO448_004188"
     CDS             4447..>4799
                     /locus_tag="OO448_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6339224.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENAST"
BASE COUNT         1311 a         1229 c         1096 g         1163 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaat
//
LOCUS       DAJTVJ010000222         4598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000222 DAJTVJ010000000
VERSION     DAJTVJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.224"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2163
                     /locus_tag="OO448_004189"
     CDS             <1..2163
                     /locus_tag="OO448_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6339225.1"
                     /translation="KGTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKA
                     SGMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMR
                     EMMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGK
                     PAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLS
                     SVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVY
                     EIDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYEN
                     VIAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCF
                     GAGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSAS
                     ELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKV
                     CSKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLA
                     GNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLA
                     NIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVI
                     HEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2397..3680)
                     /locus_tag="OO448_004190"
     CDS             complement(2397..3680)
                     /locus_tag="OO448_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6339226.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3815..4507)
                     /locus_tag="OO448_004191"
     CDS             complement(3815..4507)
                     /locus_tag="OO448_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6339227.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         1094 a         1139 c         1192 g         1173 t
ORIGIN      
        1 aaaggcacta ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa
       61 cttaaaatta agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag
      121 accgctaaag cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat
      181 aactcactct gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg
      241 gcagctcagc gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat
      301 atgcgtgaga tgatggcagg cgggggcaaa atgatcctcg ggtcagacag ccacacccgt
      361 tacggtgcat tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg
      421 cttaatgaca cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa
      481 cctgcgccgt atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa
      541 aacggttacg tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct
      601 accgatttcc gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc
      661 tggcaaactg atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac
      721 tgccagctta accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc
      781 gccatcaaac caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca
      841 ctgaaccaga acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg
      901 cacggtaaag ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag
      961 caggggatta tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat
     1021 gcactgcgcg gtcaatcctg tggcaatgac accttctcgc tggcagttta cccgtcatca
     1081 cagccggtgt ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc
     1141 gcaatcatca gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac gccaatcaac
     1201 aacggtttga gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca
     1261 gctaatgggc agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg
     1321 gcaaacggtg gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag
     1381 tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca
     1441 actcagcaac cgctgattta cgggccgaac attaaagact ggccggaatt gggtgcgctg
     1501 actgacaata tcgtcctgaa agtgtgctcg aagatcctcg acgaagtgac caccaccgac
     1561 gaactgattc cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcggag
     1621 tttaccctgt ctcgccgtga tcccggttat gttggcagaa gtaaagcgac tgctgagctg
     1681 gaaaatcagc gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag
     1741 cagattgctg gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtctat
     1801 gcggtgaaac caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt
     1861 ggcggtctgg cgaatattgc cgaggagtac gcgactaaac gctaccgttc taacgtcatc
     1921 aactggggga tgttacctct gcagatggcg gaagtgccaa cctttgaagt gggggattac
     1981 atttacatcc ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat
     2041 gtgatccatg aagatgcgcc ggtaacggaa attacgctct atatggaaag tctgactgct
     2101 gaagagcgcg agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg
     2161 taaaaagcgc catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgcttgc
     2221 gcatcttatc cgacctacga atcgcatcga atctgtaggc cagataaggc atttacgcag
     2281 catccggcac ttattgtcgg atgcgatgct tgcgcatctt atccgaccta caaatcgcat
     2341 cgaaccgtag gccggataag gcgtttacgc cgcatccggc aaatagttaa ttgctcttac
     2401 ttcttcgcct ctgcaaccac tttactaccc acgccgcggt tattgtattc ccacatgcgg
     2461 ttgtagttag tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta
     2521 ttacccgcaa acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa
     2581 ccttcgttga tggcgctatc acggatcacc acctgaccgt tggtattggc atcaacatcc
     2641 agcgagcggc ccagttgcgc cacaccatca ccgaaagcat tgaaacggct gtttacggcg
     2701 aggaaaccgt agtacatgtt ggacagcgta gccggtgcaa acacatacgc ttcttgctga
     2761 gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa
     2821 acgatatcca catccccttc aatgtagctg ttggtcacca gcgtacgcgg ctgacgattc
     2881 gtttccagac ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga
     2941 atgttaacgt tgttaatctg tacctggtca ccatcagtac gcagtgccac cgccggatgg
     3001 ttacctgcat ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagg
     3061 ccattgtttt gtgaccagaa gaccgcagag cagagaacac cgatactgtc gctgcgtttg
     3121 ctctggcagc tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg
     3181 ttgacgtcgt gacgccagtc ggcagggctc atgccaccat caagggaaag cccaatcttc
     3241 acatcaatcg gtttttcacc tgtaccgtac agagtaattc cacccggagc ggcagggaca
     3301 tataccgttc cctgatactc accaggcatc acggcaatat actggcgctt gttggtacgc
     3361 ttgataattg ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtgccc
     3421 gccgggccga caacaaagtc aggttgcgca ggcagggtaa tcggggaagg attccacgct
     3481 gcagcacctg gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg cgcttctttt
     3541 gccgacagaa tcgggcgaga agaggtacca ggcgcggttt gatcagaagg acgttgatcg
     3601 ggcggggttg agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg
     3661 gaaactgaaa atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg
     3721 aagcctgctt ttttatacta agttggcatt ataaaaaagc atcgcttatc aatttgttgc
     3781 aacgaacagg tcattatcag tcaaaataaa atcattattt gatttcaatt ttgtcccact
     3841 ccctgcctct gtcatcacga tactgtgatg ccatggtgtc cgacttgtgc ccgagaagat
     3901 gttgagcaaa cttatcgctt atctgcttct catagagtct tgcagacaaa ctgcgcaact
     3961 cgtgaaaggt aggtggatcc ccttcgaagg aaagacctga tgcttttcgt gcgcgcataa
     4021 aataccttga tactgtgccg gatgaaagcg gttcacgacg agtagatgca attatggttt
     4081 ctccgccaag aatctctttg catttatcaa gtgtttcctt cattgatatc ccgagagcat
     4141 caacatgcaa tgttgtaggg atggcaattt ttacgcctgt tttgctttgc tcgacataaa
     4201 gatatccatc tacgatatca gaccacttca tttcgcataa atcaccaact cgttgcccgg
     4261 taacaacagc cagttccatt gcaagtctga gccaacatgg tgatgattct gctgtttgat
     4321 aaattttcag gtattcgtca gccgtaagtc ttgatctcct tacctctgat tttgctgcgc
     4381 gagtggcagc gaccggtttt gttgttatat ggccttcagc tattgcctct cggaatgcat
     4441 cgctcagtgt tgatctgatt aacttggctg atgccgcctt gccctcgtct atgtatccat
     4501 tgagcattgc cgcaatttct tttgtggtga tgtcttcaag tggagcatca ggcagccccc
     4561 tccttattgc tttaattttg ctcatgtaat ttatgagt
//
LOCUS       DAJTVJ010000223         4591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000223 DAJTVJ010000000
VERSION     DAJTVJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.225"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(98..856)
                     /locus_tag="OO448_004192"
     CDS             complement(98..856)
                     /locus_tag="OO448_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6339228.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(864..1967)
                     /locus_tag="OO448_004193"
     CDS             complement(864..1967)
                     /locus_tag="OO448_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6339229.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1977..3154)
                     /locus_tag="OO448_004194"
                     /pseudo
     CDS             complement(1977..3154)
                     /locus_tag="OO448_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3222..4247)
                     /locus_tag="OO448_004195"
     CDS             complement(3222..4247)
                     /locus_tag="OO448_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6339230.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1267 a         1189 c         1085 g         1050 t
ORIGIN      
        1 gggtctcccc atgcgagagt agggaactgc cagacatcaa atcaagcgaa aggccatccg
       61 aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc tgcgataaaa
      121 atgccttcgt acgctctgat ttaggatgcg caaaaaattc atcaggtgca gcttgctcca
      181 ctatttcccc acgatccata aaaatcaccc ggtcagcgac ggttcgcgca aaccccatct
      241 catgcgttac acacaacatt gtcatacccg attgcgccag cccaatcatc gtatccagca
      301 cctctttcac catctccgga tcgagcgccg acgttggctc atcaaacaac ataattttcg
      361 gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacca gaaatttgtc
      421 cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc atcgccagag
      481 cttcagcctc tttctttggc atcttgcgta cccaaatcgg tgccagggta cagttctgta
      541 gaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact tcctgcctga
      601 cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctactacg atccgtccct
      661 gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg gaaccagaag
      721 ggccacacag aacgatccgt tctcccagtt gcacggttaa atttatattt ttcaaaacat
      781 ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta gcaggttgca
      841 gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa acgtttttcc
      901 agatgctggc tatagcgcga catgctgaaa cagaagatcc agtagatcag tgcggcgaaa
      961 acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg ctgaacgcta
     1021 ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa gagtgcgatg
     1081 atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat aaccagcccc
     1141 tgagttttcc agtaacccaa tgccagtgac tctgccgctt catattgccc tttaggcagc
     1201 gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag gatcacgcca
     1261 accagcgcgc ggatcagttt gtcgatattg gtgccttctg ccataaacaa aggcagcatg
     1321 accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc gataaaaatg
     1381 accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag taagatcccc
     1441 cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt cagcccgccc
     1501 cattgccggg tttcaacccg ctccagacca aaaaaaccgc catacatcag ccaccagaca
     1561 atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg gagtattttc
     1621 cagaacattg gtgcgattga cacaaggcca atcagtaatg ccaggttaat tcgccagcgt
     1681 tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat gaagacccaa
     1741 caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccaattagc ctgcaaaaat
     1801 gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc aatagtcagc
     1861 aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc gcggcttgag
     1921 ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat gactccttag
     1981 cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat agtcaggctg
     2041 ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc cgtctggttc
     2101 agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc ggcaagagag
     2161 gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac tcgcagtgcc
     2221 tggggaataa tgacctagcg tagcgtaacc gggttgggta atcccagtga ccgagccgct
     2281 tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc gataaatgca
     2341 gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa aaccatcccg
     2401 ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc gccaaaaagc
     2461 cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca ggtgcggcgc
     2521 agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac agaaaggact
     2581 atagccataa caacagccag gataaacgca ataaatccgt ctcccagctg cggtgacgga
     2641 atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc ctggcgtggt
     2701 ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag cgggggaata
     2761 ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt atccgaaagt
     2821 ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa tgcagaaacc
     2881 agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc ctgctggtaa
     2941 tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc aaaacctgaa
     3001 gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaaca atgccgacaa
     3061 cagcaacaac ggcaaggatc tggaataacc aggcacgaac cgtagggttg gcaaaagaga
     3121 gtgagccttt aacggttgag cggcgatgag acatagaaac ctcggaaagc ataacgctga
     3181 agagagcgcc gccgtagcga cgctcgttac ctcacaccga cttaacgcac gggcggcgcg
     3241 tactgaatac cgccgttatt ccagagatta ttttgcccac gtttaatttt cagcgggctt
     3301 tctgaaccta cgttacgctc gaaaatttcc gagtagttac ccacctgttt gatgatgtta
     3361 taggcccatt tattatccag cttcagatcc ttgccgtaat cgccttcttt acccagcaga
     3421 tgtgccatat caggcgttgc tggattagcc gctttttcat cgacgttctg ggaattgatg
     3481 cccatctctt cagcattcag catggcgaaa agcgtccagc gtacaatcga gaaccattca
     3541 tcatcgccac gacgaactac cggcccaagc ggttctttag agataacctc cggtaagaca
     3601 atccattcag ctgggttgct taatttgatg cgcagggcat acagttgcga ctgatccgag
     3661 gccagcgtat cgcagcgacc agattccagt gccttcgctg attcgtcaga gcgatcgaaa
     3721 gtcaccggtg tgtacttcat attgtttgcc ttgaaatagt cggcgacgtt gagttcggta
     3781 tctgtacccg cctgaataca gacggtagcg ccatccagtt ctttcgcgct ttttagcccc
     3841 gctttatcgt gcgtcaggaa gccaatgccg tcgtaatcag tgacgccagt aaatgccatt
     3901 cccatcccgg catcgcgaga tgaagtccag gtcgtattac gggagagcaa atccacctcc
     3961 cctgactgta aagcggtgaa gcgttctttt gcagtgagcg gggtatattt cactttcgtg
     4021 tcgtcaccaa atacagcagc ggcaacacca cgacaaacat ctacatcaat acctgaaaac
     4081 ttaccgtctg catcggcata agagaaccca ggtaatccat cactgatccc gcattgcaca
     4141 aaaccttttt tctgtacggc atcaagcgtc gcgccagcat gcgcctgatt tgcaacggca
     4201 agcagcacgc tggcggcagc cagtgtggct atcatcatct ttttcataat gcttcctgtg
     4261 aggcgaaaat tatcgttata ggacgtttgg gaaacgtctt ttacctgtct gtggccatcc
     4321 tcttataagc aaaagtagtg ccatcttttg cgcacgattt cgcgtcgcaa taagctgagt
     4381 gtagacagga aataataaaa acagcgcccc gcaatggtgc gaaaatgaaa catgcgccac
     4441 attttggcgc aaaatattta cgcgtgtttt actaagcaga ataatgattg ttagcgtgag
     4501 tatatttctg gtgggaggtg tgaaagttaa attgatgcgt tatttcgaaa gcagcgaaat
     4561 aacgcgatat aacatgctaa ggtaatgact c
//
LOCUS       DAJTVJ010000224         4466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000224 DAJTVJ010000000
VERSION     DAJTVJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.226"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..258)
                     /locus_tag="OO448_004196"
     CDS             complement(<1..258)
                     /locus_tag="OO448_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339231.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTP"
     gene            487..1095
                     /locus_tag="OO448_004197"
     CDS             487..1095
                     /locus_tag="OO448_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6339232.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1193..2584
                     /gene="selA"
                     /locus_tag="OO448_004198"
     CDS             1193..2584
                     /gene="selA"
                     /locus_tag="OO448_004198"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6339233.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2581..4425
                     /gene="selB"
                     /locus_tag="OO448_004199"
     CDS             2581..4425
                     /gene="selB"
                     /locus_tag="OO448_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6339234.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1003 a         1134 c         1319 g         1010 t
ORIGIN      
        1 gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca gcggggcggg
       61 cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcaggggat tgaccggatg
      121 gccggacgtc actccgccgg ggcacaccga acaggccaca ccggtggggg caccgatgcg
      181 caccccggct gaaccctgaa caatgctacc gccatactgc gtcatgtcgc cctgacgcgc
      241 tgccggtttt ccgctcatct gctcatcctt gttttatcgg gttatttgtc tgcttccagg
      301 caaaacccta cagatttata agaaagcctt aagtgagata tccatcagat agaaacaatt
      361 aggaaaatat ttatatcacc atgataaata tgtgatttat ttggaagtaa caaaccatca
      421 acattttctg ccctttttcc catcaccagg ccatactgat atttttcctc gcacaaagga
      481 ctcggcatga aactcgtcgg tagctacacc agcccgtttg tacgcaaact ttctattctg
      541 ttgttagaaa agggcataac tttcgaattt attaatgaac tgccctataa cgcggacaac
      601 ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa
      661 tgctggtttg attcgccgat catcgctgaa tatattgagt taatgaatgt cgctccggcg
      721 atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca aaattgaggc gctggcggat
      781 ggcattatgg atgccgggct ggtatcggtg cgtgaacagg cgcgtccggc ggcgcagcag
      841 tctgaagatg aattgttacg ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa
      901 ggatatctgg tcgatggcac actcaaaacc gatacggtca atctggcgac tatcgccatt
      961 gcctgtgctg tcggatatct caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc
     1021 ccacacttag tcaaactggt cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa
     1081 ccgccaaagg cttgatgcgc gatatgtcct cctgacccat ctcacgttac aatccgtggt
     1141 tatgttaaac gcccttctcc gtgtgagagg gccttgatca gccaggtttc ctatgacaac
     1201 cgaaacgcgt tccctctata gtcaacttcc ggctattgat cgcttattgc gcgatagctc
     1261 cttcctttct ttgcgtgata cttatggtca cacccgcgtg gtggaattgt tgcgtcagat
     1321 gctcgacgaa gcgcgagaag tgattcgtga cagccagacg cttcctgcgt ggtgtgaaaa
     1381 ctgggcgcaa gaagtcgatg cccggttgac gaaagaggcg cagagcgcgc tgcgtccggt
     1441 gatcaacctg acgggaaccg tgctgcatac caaccttgga cgagctttac aggcggaagc
     1501 cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg accctcgagt acgatctgga
     1561 cgacgccgga cgcggacatc gcgatcgggc gctggcgcaa ctgctgtgcc gtattacggg
     1621 ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg gtgttattga tgttggcggc
     1681 cactgccagc gggaaagagg tggtagtatc tcgcggcgaa ttggtggaga ttggcggcgc
     1741 gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc ctacacgaag tagggaccac
     1801 caaccgcacg cacgcgaatg attatcgtca ggcggtgaat gaaaataccg cactgttgat
     1861 gaaagtacat accagtaact acagcattca ggggttcacc aaagcgatag atgaagcgga
     1921 actggtggcg ctcggcaaag agctggatgt tcccgtagtg actgatttag gcagtggctc
     1981 gctggtcgat cttagccagt acggtttgcc gaaagagcca atgccgcagg agttgattgc
     2041 ggcgggcgtc agtctggtga gcttctccgg cgacaagttg ttaggcgggc cgcaggcagg
     2101 gattatcgtt ggtaaaaaag agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc
     2161 attacgcgcg gataaaatga ccctcgcggc gctggaagcc acgttgcgtc tttatttaca
     2221 ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg cttacccgca gcgcagaggt
     2281 cattcaaatc caggcacaac gtttacaggc tccccttgcc gcacattacg gcgcggagtt
     2341 tgcggtacag gttatgccat gtctttcgca gattggcagt ggttcgctgc cggttgatcg
     2401 cctgccgagc gcggcattaa cgtttacacc ccatgatgga cgcggtagcc accttgagtc
     2461 attagccgcc cgctggcgtg aattgccagt gccgatgatt ggtcgtattt atgacggacg
     2521 attgtggctg gatttacgct gccttgaaga tgagcaacgg tttttggaga tgttgttgaa
     2581 atgattattg cgactgccgg acacgttgac cacggcaaaa caaccttatt gcaggtgatt
     2641 actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc gcggcatgac catcgatctc
     2701 ggctatgcct actggccgca gccggatggt cgcgtgccta gttttatcga cgtacccggt
     2761 catgaaaagt ttctttccaa catgctggcg ggcgttggtg gtatcgatca cgcgctgttg
     2821 gtggtggcgt gcgatgacgg cgtgatggcg cagacccgtg agcatctggc gattttgcag
     2881 ctgaccggta acccgatgct gacagtggcg ctgaccaaag ccgatcgcgt ggacgaagcg
     2941 cgtgttgatg aggttgaacg ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag
     3001 gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat
     3061 ctgcttcagt taccaggacg tgaacacgcc agccaacata gtgtccgcct cgcgatcgac
     3121 cgcgcattta ccgtaaaagg tgccggtctg gtcgtcaccg gtacggcgtt aagcggggaa
     3181 gtgaaggtag gcgattcact ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg
     3241 ctgcatgcac aaaaccagcc aacagaaacc gcccatgccg ggcagcgtat cgcgcttaac
     3301 atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg actggctgct tgccgatgcg
     3361 ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa cccatacgcc gctgacccag
     3421 tggcagccgc tgcatattca ccacgccgcc agccacgtta cgggacgcgt ttcgctgctg
     3481 gaagataacc ttgccgagct ggtgttcgac acgccgctgt ggctggcgga taacgatcgc
     3541 ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg gggcgcgtgt ggtgatgctt
     3601 aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc aatggctggc gtctcttgca
     3661 cgggcgcaga gcgatgccga tgcgttatct gttcatctgg aacgcggcgc ggttaacctt
     3721 gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga tgcgcgaatt gctgcaacag
     3781 cctggctata ttcaggctgg ttatagcttg ttgaatgcgc cggttgccgc ccgctggcag
     3841 cggaaaattc tcgacacatt agcgacttat catgagcaac atcgcgatga acctggtcct
     3901 gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag atgaagcgct ggtactgttg
     3961 ctgattgaaa agatgcgcga aagcggcgac atcctcagtc atcacggttg gctgcatctg
     4021 ccggatcaca aagcgggctt cagcgaagag cagcaggcca tctggcaaaa agcagagccg
     4081 ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa aagagacggg taccgacgag
     4141 caggcaatgc gcctgacgct acgccaggcg gcgcagcaag gaataattac cgcgatcgtc
     4201 aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc
     4261 gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga
     4321 aagctggcaa ttcagattct ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga
     4381 aatgatcatt tattgcgcga cgcattatta tttccggaaa aataaggaaa tgattaatta
     4441 atttttaaaa taaattaata caaatt
//
LOCUS       DAJTVJ010000225         4406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000225 DAJTVJ010000000
VERSION     DAJTVJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.227"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            94..1176
                     /locus_tag="OO448_004200"
     CDS             94..1176
                     /locus_tag="OO448_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6339235.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1365..1748)
                     /gene="quuD"
                     /locus_tag="OO448_004201"
     CDS             complement(1365..1748)
                     /gene="quuD"
                     /locus_tag="OO448_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCR6339236.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1834..1974)
                     /locus_tag="OO448_004202"
     CDS             complement(1834..1974)
                     /locus_tag="OO448_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCR6339237.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1971..2333)
                     /gene="rusA"
                     /locus_tag="OO448_004203"
     CDS             complement(1971..2333)
                     /gene="rusA"
                     /locus_tag="OO448_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCR6339238.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2330..2620)
                     /locus_tag="OO448_004204"
     CDS             complement(2330..2620)
                     /locus_tag="OO448_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6339239.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2613..2783)
                     /locus_tag="OO448_004205"
     CDS             complement(2613..2783)
                     /locus_tag="OO448_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339240.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2783..3238)
                     /locus_tag="OO448_004206"
     CDS             complement(2783..3238)
                     /locus_tag="OO448_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCR6339241.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3503..3880
                     /locus_tag="OO448_004207"
     CDS             3503..3880
                     /locus_tag="OO448_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339242.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1228 a          998 c          940 g         1240 t
ORIGIN      
        1 aaacttacat cttgaaataa tcacattgat tagatgaata tttatcgcgc aatgacatca
       61 ttttttaata atagttcaaa aaaagggctc acgatgaaaa aattaacagt ggcaatttct
      121 gctgtagctg catcagtact gatggcgatg tctgctcagg cagctgaaat ttataataaa
      181 gacagtaaca agctggatct gtacgggaaa gttaatgcta agcactactt ctcctctaat
      241 gatgcagatg atggtgatac tacttatgcc cgtcttggct tcaaaggtga aacccaaatc
      301 aacgatcaac tgactggttt cggtcagtgg gaatatgaat tcaaaggcaa ccgcgctgaa
      361 tctcaaggtt cctccaaaga taaaacccgt cttgccttcg ctggcctgaa attcggtgac
      421 tacggctcca tcgattacgg ccgtaactac ggtgtagcat acgacatcgg tgcgtggact
      481 gacgtcctgc cagaattcgg tggtgacact tggactcaaa cagatgtgtt catgactggt
      541 cgcaccactg gtgttgcaac ttatcgtaac aacgacttct ttggtctggt tgatggtctg
      601 aactttgctg ctcagtatca gggtaaaaat gaccgcactg acgtaactga agctaatggt
      661 gatggtttcg gtttctccac tacttatgag tatgaaggat tcggtgtagg tgcaacctat
      721 gctaaatctg accgcactaa taatcaggtt atctacggta acaacagcct gaatgcatct
      781 ggtcaaaatg ctgaagtatg ggcagctggt ctgaaatatg atgcgaacaa catctatctg
      841 gctaccacct attctgaaac ccagaacatg actgtttttg gtaataacca tattgccaac
      901 aaagcacaaa acttcgaagt agttgcacaa tatcagttcg acttcggtct gcgtccgtcc
      961 gttgcttacc tgcaatctaa aggaaaagac ttgggtgcgt ggggtgatca ggacctggtt
     1021 gaatatattg atgtaggtgc aacctattac ttcaacaaaa atatgtccac ttttgttgat
     1081 tacaaaatca acctgattga taagagcgat ttcacgaaag catctggcgt tgctaccgat
     1141 gatatcgttg ctgtaggtat ggtttaccag ttctaatttg attactaaaa gatatgttgc
     1201 gggaggcttt gcctccccaa catataagtg gctccctcaa gccacttcct ttaggagcac
     1261 aaccttgctt ctaactatat aaaccttctg ttatatatta ccctttattt ttgggggcgt
     1321 tgcaacgccc catttttaat aatttttagt aaacaattgg catattaatt agagttatta
     1381 acaacgatat ccatctctaa ccggatatct aatgccatta acatcccttc aattatgccc
     1441 tcagccttct gtaacctttt cccgatataa ccatcagagc agcaatgctt acctgccagt
     1501 gacatgaatg tcataccgac tacataataa tctactaata aatcgtgcaa atcgctgttg
     1561 ttctttttca gacgggccat gcacccgcaa ataatcatcg cgtcatcgtc acaacattgc
     1621 gggcgagatt ttacttttga agtaattaat cccttaaaac cggcggcaat ggacgaccag
     1681 gtcacatctt catgattatt agccgcccac gctccccaac gctcaagaac catctgaata
     1741 tcacgcatca acttactcca caaaaatcag accagaacgc caattacaag caaaaatcaa
     1801 caaaacagta ttagttgatt gttatctctg acttcatact cctgctcctg tcagggtttt
     1861 ggcgtaattc ttcagtattc ggtaatcggt caaaacagaa ccggggaaac gatataagcg
     1921 cagatgcccc cagcggtggc gaagaagttc tgccatataa aactcaaaca tcattcattc
     1981 cccatttcgg tgatggtcag ttccagcctc ccacctttgg taacaggcat cttcacaacg
     2041 cggtaatcaa cgacctgagc atcatccagc cagaaacctg ctttggtgag tgcgtcaaaa
     2101 gcggcttttt gcagattatc caggtcacgg cgacggcgat ccggcatgtg gcactcaata
     2161 cggattttca ctggcatagc caggccgata tccagcattg cgtttttaat gattcgggtg
     2221 acgttatcgc ggtatgcctg cccttctgcg ctgatgtgtg tgcgcccgcg attatggcgg
     2281 taatagcggt tattgctcgg aggccagggt aatgtgatgc tgtaggtatt cacgccttaa
     2341 taaccccctc tttcagccag ataacctgcg ttctcgccat accttccagc gcgcattctt
     2401 ttgcatactc agcatcgaca aaatatgtgc ggcggtcgat ttcgtcgtga caggcagaac
     2461 atgcaatggt ggcaatcagg tctggcggtt tgataccggt accgcacaat ccagccagcc
     2521 ggatatgtgc cagtactgac gtttcaggat tgccattaca tacgccaggg attcttacct
     2581 ggcattcccg accacgcgct gcttttctca aatcagccat gattcctcct tgctgccagt
     2641 cgcaaccatt ttttatcaac caggctagcg gtatatccga gcagtgttgg tatttcggat
     2701 ggcttcagct caggcttacg cttacgacga tttgatactc tgtagatgtg cccgttcatg
     2761 acacgaataa gcggtgtagc cattacgcct cctgcttatc gcgcagctgc tggtattcac
     2821 agctctgcgg aattgtcagg tggcagccga tattcatcgc ccaggcttcg actttgcaca
     2881 ggaagatgta catctcgccg gtttccagct ctgacgtatg gcggagggat tggacggtgg
     2941 tgacctcgcc ggacacgaca tctacacggt ctttgctttc gtagccgaga taggtgtgct
     3001 tcatcgcgtc tttgacccac tcaggcgtag cgaaggtctt gccgcgggcg atgagatatt
     3061 cgctgatctc cgtgtaccac atgtggctga gcgcgttctg cgacaggctg cgcttctcgc
     3121 gccacggctt aacctgcagg cgtaagcatt gcccggcatc cagcaatggc tgaatctgct
     3181 ggccgatggc cgcgaagtta ccgcgatgga gtttgatgcc gtctactggc agagtcatac
     3241 ggcctcccta acggaaaccg cagaatgcag aaaatcgcag gtgcatttct gcatctgtga
     3301 caaggtgatg agttcagatt gtggtcgcat ttaagtcccc ttaaatgcgc agaagtcacc
     3361 ggagttgttc aggctccgat gagaggatta tggcggattg attacgataa atcaaaacta
     3421 tcttgaagcc aaaacagtga tcttcgcttt aaaaaccttg tatactttca cctaatcaca
     3481 gccagaacta tggatctaac caatgaccaa caaagaaaac aaagataaat atattaacaa
     3541 gttcaagaaa gagctttcaa aatatataca accaactgct ggagtggaca ttcaattatt
     3601 taactctaag gatggtggtg gtgtaattaa agccactttg aatagaagtg gaaaaagaaa
     3661 atcatctatt gctggtaatt ttactaaatt aggtgaggca attacctcga gtgggcagag
     3721 ggtttttggt ggtgatctat caaatgtgaa tttttacgga acaaatacga tttttgatgg
     3781 agatacaata tttttaatta aatcacctga ttcagaggag tggtcatcac aaaaagcttc
     3841 caaagacgta gagggcatcg tatttggagg gaaaaaatga ttaacattac tactaaggat
     3901 gcactcaagt cagtggtgaa gtaagagatc tagtaattct tcttgaaact gatggaaaaa
     3961 cggtgtctga ttatttgcag ccaatccctc ccaaaaaaga tacacactgg agcatgattt
     4021 tcattccggt agcaatttat atactactta atcttgtata cattattgct cacccattat
     4081 ttcctgaaaa atatctaaaa acatcaacat ctatatttct attcatttcc ttcatattaa
     4141 gtgcaatagt agcatatttt gttttccata agcataaatc tgccactcta tccatatgca
     4201 gcttagtgtt cttagtgtta attgtaggtg ctaacgtagg gtttatcagt tacagcgacc
     4261 taacacaaaa agcgacgcaa aaattagata gtttgtctaa ataattatgt tgccatgcga
     4321 agtatgcatg gctgcatgtc tgccttccat ttaaaatggg ctaagaccta taaccctaaa
     4381 tattattctt tattatcttc tttacc
//
LOCUS       DAJTVJ010000226         4404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000226 DAJTVJ010000000
VERSION     DAJTVJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.228"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(139..3654)
                     /locus_tag="OO448_004208"
     CDS             complement(139..3654)
                     /locus_tag="OO448_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6339243.1"
                     /translation="MDKNALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="OO448_004209"
     CDS             complement(4106..>4404)
                     /locus_tag="OO448_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6339244.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1128 c          905 g         1230 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAJTVJ010000227         4362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000227 DAJTVJ010000000
VERSION     DAJTVJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.229"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            649..1526
                     /locus_tag="OO448_004210"
                     /pseudo
     CDS             649..1526
                     /locus_tag="OO448_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1526..1816
                     /locus_tag="OO448_004211"
     CDS             1526..1816
                     /locus_tag="OO448_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339245.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            2137..2427
                     /locus_tag="OO448_004212"
     CDS             2137..2427
                     /locus_tag="OO448_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339246.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2427..2846
                     /locus_tag="OO448_004213"
     CDS             2427..2846
                     /locus_tag="OO448_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339247.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            3014..3337
                     /locus_tag="OO448_004214"
     CDS             3014..3337
                     /locus_tag="OO448_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339248.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            3767..>4362
                     /locus_tag="OO448_004215"
     CDS             3767..>4362
                     /locus_tag="OO448_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339249.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1444 a          786 c          899 g         1233 t
ORIGIN      
        1 cataacaggc ttactattag gtcaccgttt ccaagtgcag tcgttgtttt taaaaaagaa
       61 ccacgtaagc atgaaattac acagatggtt tgctgtgctg atacattgct accgttaacc
      121 atcattaacc gtggtggact aagaggaaga cccaccattt acccacctga gacgctcgac
      181 agcttcataa aactatacag gcaaggaaaa cccattaagt acattgcaga tgctctcaga
      241 atgcctctga gcacgtcata caggattgct caaaggttga gctaaaattc tcataaatat
      301 ggtcatttac aagacaggtg attcagatgt taagaagtat ccctgtgaac aataaatgac
      361 caatcagtaa tcattctgtg aaataattgt tgcaatttat aatcgcatct gttatagttt
      421 ctatatgggc tgataaaaaa gttcatgtct tgaagtggta acacttgggg aggtgtcaga
      481 tggttggcgt atattaacct ctcattcagg gtatacctca gcgttttaat catcgtactg
      541 tttcaggcat ggacttcttt atcagtccat caatccccca gatattttaa tcacacaggc
      601 taatcggttt tatccggttg gccttttcac gtatgaggta atttatgaat gtctaaatat
      661 aacccaaatc agttttataa atttgatgtg cgaatctctc aactttcaga agaaattaac
      721 gcagcagcaa gattgattta tatggtcatg gtccatagct gtgacttcta tgaagagaaa
      781 tacaaatcat attcaccaag tattgaccat ctcgcttata tggctggtgt atcaaagaga
      841 acagcaatca gagccattga gacacttgag aaacacggtt taattcaggt gcaaaagaat
      901 caggggcgtc aaagcactta tcgtgtattc acactggatg ataagccgga attacttgta
      961 agacctacat cacaggaagt tgcagcctca gtaagacaaa atgacaccac taccagtgac
     1021 aaaatgtcat gggggtagtg acaaaatgtc acccgtgtta gagtttagat aagttattag
     1081 ataataatat tacagataag aataaagaga ctggacttaa tgatgatatt aaagaagatg
     1141 actttctttc tttggatgaa ttaaatgatt ttctctctat gaatgaatct gaccttgaag
     1201 taagctctaa aaagacttct aaacaagaca gagaaatcac tgatgtgagt gttgatagtg
     1261 accataaaaa cacacagtgc cccgccactg atgatgagtt ggaccccttc gatgatgaag
     1321 aggatgaacg tgaaaaagcc attaagaggg cagagaagaa aaagagattc gagcttgcaa
     1381 gacaaaagaa caaagtatcg ggtttagacc cattcgatga cagtgaagat gattattatc
     1441 tttcaccgaa aaagaaacaa actcaggatg acaagaagca aaaaattaaa ccgaagatgt
     1501 attttgccgg tggaggaatt aagtaatgaa agaatatcac ggtgaaaaga gatacaaaga
     1561 ttacctgtta aagcgttaca gcatttctcg tgaaggtcat cttatgaagg atactcacgg
     1621 ggaagtttac cggattagac caaagaaaga agggaggggt tatttctttt acgatggtgt
     1681 gacagattta aagattgacg ctttgaggtt tgcagtgctg tatcattttg atatctggaa
     1741 ttcaatccac cagctacgat taaaagacgg tgaccccagc aacttaaagg ccaccaatat
     1801 tatcacaaaa cgttagtttg ttttaccatc atcaatcgta attgtgattt catatttgaa
     1861 aagcttaatg ctgaaattga atcgtgtact tctggcgata atcattttta aaagctgata
     1921 cacaagaagc accagaaaca caaagcaacc taagcttgcg aatctgtgag attcagcaat
     1981 tgtttcaaga aagcccatta attcccctta tttatcaaag ggaatgacct gtatcagccc
     2041 cctacaacca tattaacatg taaatgagtt gttggcaata tgttataaat atgttaaagt
     2101 tagttataag tcaaagattt taagaaggaa aatttaatga gacacacagt tacacgcctt
     2161 ttgccagagg catattccag actggcagca atcaaagaca aatacccagc catgagccac
     2221 acagcacttg ttggtgagat tttgaaggaa cacatagccc ttacaccgtt tgacgctatc
     2281 aagggcgttg cagagggttc taaggccatc agaatcacaa cgggccaact ggaactactc
     2341 aaagcacagt ccgcagtggc aggttgctca atgattcagg tgttgagtgc caaggttcat
     2401 caactggcgg ataagctggg ggtttaatgc tatgcacatt tgattatcat gcgttttatc
     2461 gccatagagg tcaccttgtc cgtgagaata acggaacatg gaaagattta actcgtaatg
     2521 ctgaaccctt cacacaccag caattaaaat cccgcttctc cagatggaag aatgcaccag
     2581 cagatactgg tgattttgag attatccata ccttcgatta tgcctcagaa gatgaccttc
     2641 acgatgcaat taagctgatt gaagacttca atacctcaga agatgtggat ggtgacaaat
     2701 ataaatcccg tggtaaccgc tataaccgtg atgatatcac tcaggccata aatgctggtc
     2761 aatctcagcg atacattaca gaaaaatatg gtatttcacg gagacagtat ttctacatta
     2821 agccattgat taataaagac aattaaaaat aaccatctaa gaaacggtag aatgcacttt
     2881 acaggcagag ttgcacctta atttgataaa ttaccttacc agccagtgca actcgctctg
     2941 taacccgcat ggttactggc ctcagaatgg ctattttaag aggtctgagg tgtgtttcat
     3001 aggagattta aaaatgaata attacccaat gcaaattatg gttgataacg acactgctat
     3061 gatggtgcaa agcttcattg atgcaggtat cgcaattaat tttgataaat tactaaagct
     3121 gatggctgat aatgtagaaa acatctctga ctttattcag tcggtggagt ttaacgagcc
     3181 tcgaatgatg ctaccaatta cagacagcaa catgaaaaga ttggttatcg agcaaacgaa
     3241 catatacagt atttccccag agcaattttt gaaaggtgct gtgacaatcc tgtacagtga
     3301 taatattctg gtgacggatt ctgtgagggt tcactaatgg gtttaataat tgcttggacg
     3361 ttgcttggct tatttgttgg ttgtattatt aacaatgcgg taaataagaa ttgatgttat
     3421 caacctgtta aaaagctgtt gacaaataac cttaagcatg gcataatttt aattatggga
     3481 gagcaattct ctcttatttt tctttcaata atccgatgga tggatattct gagttggaaa
     3541 ggtgcaatgc ttttcgtcat ggcctttcag gttagtctga gaggctatct caaaaagtac
     3601 tcttgttcat tcattattga atcctttgcc cttcacagaa atgtttaggg ctttttcagt
     3661 ttttaaaagc acacttggca ggttcttgtt cagtcagact cttcacctct gattgctgag
     3721 tgtgcttcta taaattgaaa taacaaataa actaaggggt gaacgaatga ttagaaatga
     3781 acatgaaatg cttatcgaac acagaccaag tgcacttgat attcacgaat taactttgcg
     3841 tgaatctttt gatagagctt ggcgggaatc acacgaagta aataaaggtc catatattcc
     3901 accagaacca ctcgagattc caagagttga aattgatttt tctatgaacg aacggtgtga
     3961 acttgatttg aagttgaagc tacaacgccg atatttccaa gctattaaag actctcaaga
     4021 agcaatgaga agtgtttacg agaaaattca gtccaagctt gaagacgaaa ttgacgaaat
     4081 ggttatttcg atggagcttc aaaccaattc ttatgcttat ttctctcgac tagaccgtga
     4141 cggttggggg tatgaccctg tagagattgg taaagtgatt gcagatttac cagaaggtca
     4201 aagagttgtt cgtacagaaa gagctgtcag agcaccacat cgactgtctc taattgttgc
     4261 tgataaatca caagctgagc ttgaagaaat tgccaaagag aaactgatga aagcaagaga
     4321 aaatgagatt aatttgctga aatcaagact ggctaagctg at
//
LOCUS       DAJTVJ010000228         4325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000228 DAJTVJ010000000
VERSION     DAJTVJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.230"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..579)
                     /locus_tag="OO448_004216"
     CDS             complement(<1..579)
                     /locus_tag="OO448_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6339250.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYTSYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(642..1091)
                     /locus_tag="OO448_004217"
     CDS             complement(642..1091)
                     /locus_tag="OO448_004217"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6339251.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OO448_004218"
                     /pseudo
     CDS             complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OO448_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1027 a         1089 c         1167 g         1042 t
ORIGIN      
        1 gttcgggatc atcgcgtcaa cggtgatggc gtcagcaggc tgtacgccgc caaccagcgc
       61 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
      121 ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
      181 caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc cgaggaaggc
      241 atacgaagta taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca ggtttacgaa
      301 ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccatgc
      361 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
      421 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagatc
      481 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
      541 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgagt
      601 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
      661 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
      721 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac ggtttctccg
      781 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
      841 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
      901 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg gtaagtcaat
      961 gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
     1021 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
     1081 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
     1141 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
     1201 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
     1261 tggagccgag gccaagcagc tggtgatcga ggttcagggt gatgtcatca ctgcgctgca
     1321 gctcgttaca gtgctgggca gcgtggatgt tttcctaggt atagtgccag gcgctgaagt
     1381 taattgggcg ctgcggaacc accagcagac cgttaccgtg gcggttagtc agtgccgtcc
     1441 agcggacatg ctgacggtta ccgttgttct gcgggaaggg atagttctcg aacatggcat
     1501 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
     1561 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaattccc atggtgaaac
     1621 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
     1681 cctggccatc ggcagcgatg cgccagatgt aggtgcagcg cattccgaaa tcaaacaccg
     1741 gcggggcaat aaccgtgcgg ctgatgatca acacttcacc ctcgctctgt tctacggcaa
     1801 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
     1861 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
     1921 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
     1981 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
     2041 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
     2101 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
     2161 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
     2221 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
     2281 cctctgcgtg cagggggtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
     2341 actcgccgcg agtcagatcc cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact
     2401 ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
     2461 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
     2521 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
     2581 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
     2641 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
     2701 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
     2761 aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg cggatgttac
     2821 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgcg
     2881 cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
     2941 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
     3001 caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg
     3061 tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg acgcgatcca
     3121 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
     3181 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
     3241 gttgtggtac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
     3301 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
     3361 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
     3421 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
     3481 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacggtga
     3541 aatcgttaat atgtgttagt tgttttccga tcagataaac atcgcggaag atccccgctg
     3601 accaccacat atcctggtct tccacgtagg tagagtccgc ccattgcatc acgcgcacac
     3661 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
     3721 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
     3781 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
     3841 gataggcacc ggttgggtta tcgctgggaa caaacggcac atcgatgggg aacggaaaac
     3901 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
     3961 tatgccccca gtcagccatt aactcagagg tgaaggcttc aggcacttgc agcgggtggt
     4021 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
     4081 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgt ggcgcaagtc
     4141 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
     4201 aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt agtaaaattt
     4261 ttactaaaaa gtgacaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
     4321 cactg
//
LOCUS       DAJTVJ010000229         4322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000229 DAJTVJ010000000
VERSION     DAJTVJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.231"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(84..2033)
                     /locus_tag="OO448_004219"
     CDS             complement(84..2033)
                     /locus_tag="OO448_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6339252.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2118..2453)
                     /locus_tag="OO448_004220"
     CDS             complement(2118..2453)
                     /locus_tag="OO448_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6339253.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2453..2809)
                     /locus_tag="OO448_004221"
     CDS             complement(2453..2809)
                     /locus_tag="OO448_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6339254.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2809..4305)
                     /locus_tag="OO448_004222"
     CDS             complement(2809..4305)
                     /locus_tag="OO448_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6339255.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT          991 a         1297 c          956 g         1078 t
ORIGIN      
        1 aaaatggctt tatcacaaaa caaactccat tgttagcatg ttacgcgata attttgcctc
       61 atctttcacc acaaaaaaag aactcacctt ttaaatggag aatacccaac atcatgggtg
      121 attttcataa agggatcgcc tgttttcggc aaatcgataa cgcgcattcc aggcggcgcg
      181 ttgtcaaacg tcaccttaag ttcgctgcgg gttgccggtg aagatgcaag gcttaacagc
      241 tgattccttt ccagtgatac attcggggtg tagcgtttat cgggagataa atacccatca
      301 ccacgtggca tctgccatcc ggtcatgtca tagacaaaat catggaactg tttgccccac
      361 tcgtcgaact tatcattcag accaaatcca ctgttcagtg catcagccac aaaattttta
      421 acaagccagg gatgtgcttt ttcaaactcc tgcgccatca tgcccagttc aagcaatcca
      481 ccaatcagac tgatctttcc gaggcccttt aatttcagcc ctgtttttcc tgccgccgtt
      541 gtccattgat tctgggcgac tgttgccgca cgaatacttg ccaccgcctt cacaccaata
      601 tacagggcag aaaccgttgc tattgttttt actgcctgtt cccatccccc cattgctctg
      661 acaacctgat caacctcctg ccagactttt ttcacaaccg gaccgaccgt ttcccagtta
      721 tcaattatga gatatgcacc accgacgaga agcgcaatta atccctttgc cggcgtcata
      781 ttcatcacgc tacccatgat ttttgttatg cgtgtcaatg tgccaatcgc cacgcccatc
      841 gtcagtaatg ccgcaccagt tttcgcgatg gtttttacga cctcaggatt ttctctgaca
      901 aacgtgcgca cttcttccag aaaaggcttc atctctttta tgccttcatt gagtgaaggc
      961 aggaaggttt cccccagcgt ggaagaaatc gcattgatct ggttctgcag cagcaacaac
     1021 tgattttctg tcgtcgcggc acgggcggca tattctttct gcattgagcc gccatactgc
     1081 tgcgcatccg ccacacgatt aaagttggtt cgcaacaaat ccagattcgt gagcagcggc
     1141 gcaatggccc ccagggattc cttcccgaac agcgctttca gcacagcagc ctgtttttct
     1201 ttcggcactt ttgccagaga atccagtacg tgcagcatgg ccccgcgggc gtctttctgc
     1261 atatccgccg ccagtttttt cgggctaatg cgcaacgagc ggagcacttt tttctgcgat
     1321 ttggtggcag aatccctcgc tgtcagcgac aacataaaat tctttatccc cgtcgcagca
     1381 atttctgact ccacccccat tccggcaatg gttgccccca ttgcggcaat ctctccggag
     1441 gccacacccg caacactgcc taaagggcca atacgggtca caacatcaga aatctttttc
     1501 gcacttgcag gaccggtatt accaagatag ttaatcttat ccgcaagccc tgccacctct
     1561 ccctgtgtca gtttaaaggc agtgcgccac tgcgccatca tctgaccgga ttcttctgcc
     1621 gtggtgtcaa aggccacgcc catcttcacg gcatcgtcag taaactgcat cagctcgtca
     1681 cgggcgatac cagcctgacc acccgccgcc acaatttcgg cgataccttc cgccgacatg
     1741 ggcagttctg ttgacaggtc gcgcacctgc tccgtcattg ccttaaacgc ttccggcgta
     1801 tccagaccat ccaccacttt ccggacatcc gccatttttg attcaagggc aatggcagat
     1861 ttgaccggga gcgccagcgc ccccagtact gcggttccgg cacccgctgc gcccagtaac
     1921 aggctggcaa actccttttt aaaacctttc agctggcgct gcattccctt cagcggtgcc
     1981 gataccttat ccacggcagt gatgatcgcc ttcagctgaa aactgtcagc catgcttcat
     2041 ctcctcgtta atgcggacgg cctcggcttc cagttctgta aactgcgaaa cagccccccg
     2101 tcgcagctcc agtggattca gtttccagaa ccaggcaaca ttgtagagtc gtttccggag
     2161 gtgcttcccg tctccgactg ggtaaaaaaa cgcaggatct gcatgctggt cttaaaaata
     2221 tccagttttg ccatctgcgc cgccgatgaa cgcgggatcc ccgccagcaa cgggatatat
     2281 ttcagtgcca cctggctgtc cagtttaata ctgccttcac cggaaataat gaaaggaaaa
     2341 cccagcgcct cgatttcgtc atacgtgggc tcgcgcaact ccagcacatg cagcatttcg
     2401 ttatgcgctg tcaccggttt ttttaaaact atttctgtaa cgttcattgc cattactgat
     2461 accctccctc ttcgccgtgg aattcaagat ctgccgtgcc ttcttcggca ttatgattag
     2521 cctctccgtg cagccatgcc gccgaaagca catacaccat gccgtttgcc agctcagcgg
     2581 taatcgtcat ctggtctgag gtggtaattt tgtcgaccgg aaagtttttg ggcactttaa
     2641 acgtgccctt aacgtaaggt gaccgccagg tctccttgta atccacatcc cctgccatgc
     2701 cgacgatgtc atcccggaca ttggtgttca tcggcacttc aatgccacct gtcagcgata
     2761 actgctgacc atccacttta aaaaaacagg ttccggcgat ctttgccatt atgctgactc
     2821 ctctgaatac tgaagacgga actggttaac caccgcaaag acacgtaact gattaacata
     2881 atccggcggg aacagcgtat tcagtcggtt cgggttatct gcatcacgct caactatcag
     2941 atactgttta aacagatcgt aattttccac aataccggca cgctccatct gacgatatgt
     3001 cgccagaagt tcccctttga taacggcagg agtgacaatc gcctgccccg gaccaaaacg
     3061 ggtaccatca tttgccagct tgtgacgtcc gtacttgctt gtgatgaccg atttcagttt
     3121 gcgaagaaca tatgcactgg tatgcagagt ttcactgtcc agatagctgt tgtctgccac
     3181 gccatacgca ttctttttgt aggtggttac ggaacgctgg atctgcaacg tgccgccttc
     3241 cacataagcc gtcgccacgc cgtgagataa aagggtctgc tgctctgtca tgatgaatcg
     3301 cttacctttc ggtgccggaa gcatccccac cagctccccc gtctgtgtcg gacgggcagg
     3361 atcattccgg ataaataccg cttcacgggc aaggcgactg gcaaccagtt catcgacagg
     3421 cgactgggtt tctttttcgt aaccggcaag cgtgatatgt tgctgattat gcatatctcc
     3481 ggcaccaacc agctctgaca gcgttcccag ttttgcggta tagccatgcc cgtataactg
     3541 gcgcgcataa ctccagcgac cgctgctgtc attcatttcg gtcatcatca tattgatgga
     3601 ggcggcatcg ttgaacggca gaccgataaa gtcgaatgcc tcatcgccca tagcagcaac
     3661 agcggcggta agatcaggcg ctccgctacc ggcagcaccg acttccgtca ccacccgaag
     3721 ccctgctggc agaatctcac caccaccaga accataataa ttcagacaga ccggtaactc
     3781 attaccatac agccccttat gacgggcagt cagcgtcacc acacctgcat cagatgacgc
     3841 cgtaaacggc agggtgataa ccccgtttac cgcttcctta atcgcggtgg caaccgcagt
     3901 ggcatcatcg ccattcacca caggcacctg tacacgggag cgcccgacat acagactcag
     3961 ggtgccgctt tcctctgctt ctccggcaac cgtcaccctg accgtcgccg ccgcccctct
     4021 ggcttccggt actgcaataa catacagttc accgaaagga tctgtctgac ggtagacttc
     4081 caccatacgc gccagctggc tccccgcccc acaaatctgg cgggcataat ctgccgatgg
     4141 catcagcacc aggctgttaa cctcaatggc tgcatcgttg ctggcatgcc cgatcagtaa
     4201 tgcaggcgcg ctggtcaccg ctgtatttgc cgcagaattg ttcatctcgg cgtagaacaa
     4261 cggcaccagc gtattcgacg gaacagtatt aaaacttatc gtcatgattt tttagcctta
     4321 tc
//
LOCUS       DAJTVJ010000230         4309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000230 DAJTVJ010000000
VERSION     DAJTVJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.232"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(108..1472)
                     /locus_tag="OO448_004223"
                     /pseudo
     CDS             complement(108..1472)
                     /locus_tag="OO448_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1537..1656)
                     /locus_tag="OO448_004224"
     CDS             complement(1537..1656)
                     /locus_tag="OO448_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6339256.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1811..2029)
                     /locus_tag="OO448_004225"
     CDS             complement(1811..2029)
                     /locus_tag="OO448_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6339257.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2383..3861)
                     /locus_tag="OO448_004226"
     CDS             complement(2383..3861)
                     /locus_tag="OO448_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCR6339258.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1220 a          942 c          760 g         1387 t
ORIGIN      
        1 cctaagacat caatcatctg ctctccaatg actagtctaa aaactagtat taagactatc
       61 acttaaataa gtgatactgg ttgtctggag attcaggggg ccagtctagc aactaaagca
      121 ctgcccgtct cctggttaac cccatgtgct ataccgccgg tatagacaat cagcgagcct
      181 ccggcgctaa tattgctgcc agttgccgta ccatctttct caacaacctg ccggctcccg
      241 gtggaaatat ttgtcgtgtc agctttcccg ccgctcttga tattttgtgt tccgctgttg
      301 atattggtac ccgtggcaat accatgatta ttaatattct gtgtaccacc attgattatg
      361 gtattggtcg cgtttctggt aacatccatt accccgccgt tatctattcg ggtcgcatca
      421 gctttagcat tcgttaaaac tgacattgtt cctttatctt taataatcgt cttatttgcc
      481 gaaccatatg cgtttatgtc taaatgacca ccgttttcca gcaacacatt gtctgccacg
      541 tgattgtgga tggagaatgc accttcacta ttcgtaccgc tcaccgtcgt accgttagtg
      601 tttgttttta aaattgcacc atcgtgctgg gtaacatttg ttgccgtacc accactaaca
      661 tcaagcacgc caccggaata aacttcaata acatccgagg agctggtgta atcaacaatt
      721 tgcgtaccac cagaatagat ctgagtattt tttgccgttg acttattatt cagagactga
      781 gttccacctt caatcgtcgt gtccagcgca tggctttcat atactctttg ctcaccgcca
      841 tttttaatgg tggttgttcc tactgtgctc tgttcaacat actgtcgtcc accatttatg
      901 attgtgttcg ttgccatact tccttgcgtg atgtcctgat aaccggactt atttatcgtt
      961 gtaccatcaa catgcccctg agttgttact gtctgccctc caccatcaat gatggttcca
     1021 ttcgcagtcc ccccacttat gtgtgaatta ccacccttaa ttgtcgttcc attgctgata
     1081 ccgcctgtat aaacgtcctg attgccactg tcgattatcg taccggtgga aatgcccccg
     1141 tcatgaattg tctgtcttcc tccgttaatg gttgtattat tagactgccc catataattg
     1201 ttattccttc caacatcttg atatccacca gattcaataa gacttccatt agatactccg
     1261 ccataaacac tctgctggcc atggttgata atatgagtgt tatttgttgt acctcgttca
     1321 tctactcgtt ggttgccatc tacagtctca tcgtttacca caccaataac attagtagta
     1381 aaggccgcca tcacgggcgg ggcatatatc aaggcagata tcaataagga aagtactgag
     1441 cggcgacaat aatggggact ggtcctgttc ataaatttca tcctctgaaa agtgaatact
     1501 gagtagcgtt taagcgactt tagctctgct gcaacatcag cccgcaggca ccagaccggg
     1561 gggttcatcc tgaagagaca gcgcaagtgt attgtgttca ccgctcatca aagacatcat
     1621 gatgaaatga tgatgttcca cataagaaag aggcattttt taaacacagt gcgctgaagt
     1681 gtgattggat aaaaaagtca atctattcag gaaatacggg cgtattcttt tctttcgaca
     1741 atcaggccgt cggcaaaata aaatgattta cataatcgtt tctgatgaat atcttctgct
     1801 cacataaaaa tcacacaata actttgagat cgcagattct tttactttta cagcattcgt
     1861 ccccccattg ttgggcaaat atagattggg ccagagcacg aaagttaata ccacgtttgc
     1921 acagctcctc caacagcacg acaagatgcc acatactgcg ccccagtcgg tgcggtttac
     1981 agactacccg tgcgtccacc gccgataatg tcctgagcag ttttttcagt ccggacctcc
     2041 tgccacggtt ttacgttgaa gaggtaaccc tgagcacgca gttcttcagt caggcgtggt
     2101 gcaccgtaac gctgttattg ctgggtaaga tcaaaaaact ttcaggcagc taaggaaagt
     2161 tgaaccagac attagatgaa atatttcaac caattacagc accaatttaa actcagtaaa
     2221 tcacagcaca gatcaagggg ttacgtgaaa gcgtagcccc tttttctttg gtagtggcag
     2281 caaaatggtt gtagtgtaaa aaataatccc gtttaatcaa tcaataatac atattgtttc
     2341 aatctacgtt attatctctt tgtaaaaata gccatttatt aatcattgaa aactgctttt
     2401 agaacttgat acaacgggac tagtcacaac aggactattc tcaacgggat catcctcaga
     2461 ggaactatca tcaaagtcat catccataaa taaaatatca tcgaatggtg ccacgcccgt
     2521 gatgagtttt attttattat tacgatcagt caagactcca cttaaaccgt tttcgctcac
     2581 aggttttaat gatttttcat tactcttgtt gtaagcaggc gcattaaaaa tacacggagt
     2641 atcaagatca aacaatgacg ttctccagtt cacatattga atatcatagt tactgaagtt
     2701 ctgtccagaa aagaagcatc ccttaaaatc caatccacct aaattatata aaccatcctc
     2761 ttctttttgg agagtaatgt taattctggc tatctcccgg acaaaacttc cttttttgta
     2821 tttgaatacc gtttcaagat tttcaccaga cagcttgact tctgggaata atttaaaatt
     2881 aaaccctaca tcattattat tcaaagtaga tgaacgaaag atggaaaaag cttgtagtgc
     2941 tgaattatag ctttcgattt tatcttgagg ctgcgctctt ggtaaaaact tatagcagca
     3001 ctcataaaaa ttgaataaac gttctgaaca attcccattt ttatcaatta ataccccccg
     3061 gaattctttc acaaaggcat ccgttatttt ttgaatatct atggtgtgtt cctttgactc
     3121 tgacaaagat ggagtctgtt gaagtgcatt gctcatctgg ttaaggaaca tctgcatttc
     3181 tttaaattta tctccatagt ggcctttaac ttgctgagag tttttagtta caggtgaaac
     3241 cttatctatt tggttgtcat ataaattatg tggttccata cagacatttg gttgtgtttc
     3301 taaaataaca ctacgtgtac tacctggctt aaatgaatca acatgaaaat cactttttct
     3361 tatattatcg aatagggtct gttcatttct cttagcgcat tcaataaact gatcaacatt
     3421 atttttattc gatgacaggt cagtttcaga aaccgcattt tctttgttct ccagctttag
     3481 tttaatgaga agatcttccc agacctgctt gcttaaacat actacgtcag gctcaccatc
     3541 agtaactaac tcttcattat tatcaaagta tacgttgaat acttttaagt tattttcatc
     3601 aacatcatat ttaatacgtt ttaattgctc tccagatccc ataatgacaa aggcgttgcc
     3661 ttcatgatat atacattcag acatcatttt ttgtaaagtt tcaagagcac cgcgatacgt
     3721 ccctgaggca gcttccttac aaattaaatt tataatacta taagctagta gctccatgga
     3781 ttcagaagat tttctttcat tacaatttcc actatctata aatgagctag tgttaaattc
     3841 gttgctttta ctaactaaca ttgtctattc ctcagttaat gtctacatgg ctatttttaa
     3901 ttttattatt gtttgtcact ataaaaaatc gctcatttga gacaattgct ggcattaaca
     3961 gcttcatatg ctatacatgg tacttttaat taaattagca caagaatgtt aaatttaata
     4021 aacaaaaagt tatttcgctg tatgataaaa accacccgtt ataatttatt agtgaaattc
     4081 gtttttcgag tgttagaaat ttatatctaa atagcgttga ttaatgagca aagccagact
     4141 cctgtaacgc tcacaagact catctacctg cgacgggtat tctggtcagt agtagatgtt
     4201 taaggcgtgg cagagacatt tcatccttac tctacggcat tggcccgcat acaaatcgtg
     4261 gttgtgacac tcacttatca tcagtgagcg aacagagaat agttcagtg
//
LOCUS       DAJTVJ010000231         4290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000231 DAJTVJ010000000
VERSION     DAJTVJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.233"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            225..1067
                     /locus_tag="OO448_004227"
     CDS             225..1067
                     /locus_tag="OO448_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6339259.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1070..2158
                     /gene="capU"
                     /locus_tag="OO448_004228"
     CDS             1070..2158
                     /gene="capU"
                     /locus_tag="OO448_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6339260.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2163..3113
                     /gene="virK"
                     /locus_tag="OO448_004229"
     CDS             2163..3113
                     /gene="virK"
                     /locus_tag="OO448_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6339261.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            3178..4122
                     /gene="lpxM"
                     /locus_tag="OO448_004230"
                     /gene_synonym="msbB"
     CDS             3178..4122
                     /gene="lpxM"
                     /locus_tag="OO448_004230"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6339262.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         1225 a          799 c          980 g         1286 t
ORIGIN      
        1 ttcaggtagc caaattcagt gtgccacctc aaggttctta gtgagtcgtt tttaaaagat
       61 atgataatat atcattgttt atgaattgtt gatgtttaat ttagtaaatt atatcaaata
      121 tgccatgggt ttgctatgtt attcatccag tatctggaaa taaaatgcac cagctttatt
      181 gggttttcct tttgtctctg cttactaggt gccagaaact ttaaatgtta aacgaagggg
      241 gcattttgtt caaagccaat catgttccgg tattaatgta tcatcatgtc agtcattgcc
      301 ccggactggt tacactttct cccgtgacat tccggaagca aatgaaatgg ttggctgaaa
      361 acaactggaa aacactgtca tcagatgaac tggagttttt ttatcggggc ggaaaactcc
      421 cccggaaaag cgttatgttg acatttgatg atggatacct tgacaactgg tttcaggtat
      481 atcctttatt aaaagagttt aatctcaaag cacatatttt tttaattacc ggttttattg
      541 ggaatggtcc ggttcgtcac tctcctggaa aggaatattc tcatcgcgat tgcgaacatc
      601 aaatagcgac agggaatgct gataatgtta tgcttcgctg gtctgaagta aacgaaatgt
      661 tgcaatctgg tctggtcgaa tttcatgttc atacgcatac tcatacacgc tgggataaaa
      721 agttcagttc acgagaagaa cagtgtaaac atcttcggca ggacttgttg agcgggagag
      781 aatatttgaa agaaatgaca ggaaaatgca gtaaacattt gtgttggccg gaaggttatt
      841 ataacaagga ttatattcag gtcgctgaag aacttggatt ctattattta tatacaacag
      901 aaagaagaat gaatgctcct gctaaaggaa ctacacggat aggaagaata agcaccaaag
      961 aacgggaaag ttgcgcatgg ctgaaaagac gactttttta ttatacaaca ccgttttttt
     1021 catcgttgct ggcctttcat aaggggccaa gattaccgga tgattaataa tgaatatact
     1081 atttacggaa tcatcaccga atataggtgg tcaggaatta caggctgttg ctcaaatgaa
     1141 ggccctgaag aaaatggggc attcagttct gcttgtctgc agggaaaaca gcaaaattgc
     1201 ttttgaagcc agtaaattgg gaattgatat cacattcgcg ttatttcgaa acagtcttca
     1261 catccctact gcatggagat tactcggaat agttcatggt tttcagccca atgcaatcgt
     1321 ttgtcacagt gggcatgata gcaatattgt tggtttagta cggttattca cttggaaaca
     1381 tccattcaga attatcaggc aaaagacata tttgacacga aaaacaaaag ttttttcaat
     1441 aaatcatttt tgcgatgagg tgattgttcc cggaacaagt atgaagacac atctggagca
     1501 ggaaggatgt cgaacccggg ttactgttgt gcctccaggc tttgatttcc agaaattata
     1561 cgttgattcc cgaaacagtt tgcctccaaa tgttctttct tggctggcgt cccgaagggg
     1621 atgccccgtt attgctcagg taggaatgtt gcgcccggaa aaagggcacg aatttatgtt
     1681 gaatttactg ttccatttaa aaatgaatgg acgacagttc tgttggttga ttgtggggtc
     1741 tggttcgcct gaactgcggg agcatttaca gtatcagatt gacagtatgg gcatgcatga
     1801 tgatgttttt attgctgaca atgtttttcc tgccgccccc gtatatcggg ttgccagtct
     1861 ggtggttctg ccttcagaaa acgaatcgtt tggtatggta ctggcagaag catcggcatt
     1921 ttctgtgcct gtactggcca gtcagattgg tggaatccct gatgttattc agaacaacca
     1981 gaccgggaca ttgttaccag caggtaataa gcacgcatgg atgtgcgccc tgaatgattt
     2041 ttttaatgac cctgggcgtt tttatcagat ggctcgccag gcaaaacagg atatagaaga
     2101 gcggtttgat attaataaaa ctgcgttaaa aatactcaca ttagcgaagc acaagtaaca
     2161 atatgttttc tgtaagtaac ttatcattta tcggtttcct taaaaggatt gttttttcct
     2221 cagattcact cccggggaag tgggaacaca gaaaattccg gttcatgtac attttgcgat
     2281 gtgctataaa tccggttgcc agtattcgat attattacga actgcgttcc ttgcagtgca
     2341 ttgaggatat tctggcaata caacccacgt tgccagcaag aattcatcga ccttatttgc
     2401 ataaaggggg gcgagcctgg tcccgggggc aatatattct tgagcattat cgctttgtgc
     2461 aaaatttgcc agaaaaatat tctgaatttt tgtttccaca aaaatcggta tcattagtcc
     2521 aattcatcgg gaaagatgga gaggattttg atatccagtg ttctcccagt ggttttgacc
     2581 gggaaggaga gttgatgttg tcattgtttt tcaataaaat agtgattgcc cggttaacat
     2641 tttcagtcat tctgactcaa aatggacaca ccgcttttat tggcggatta cagggagccc
     2701 ccaaaaatac cggacctgat gttattcggt gcgcaacccg ggcctgttat gggcttttcc
     2761 caaaacggat tatttttgaa gcattttgtg cgttgatgaa agcttgtaat gtctctgagt
     2821 gtctggcagt cagtgagcac agtcatgttt tccggcaatt gagatactgg tatcagaaac
     2881 gcaaaacctt tgttgctgtc tacagtgatt tctgggagtc tgtagcaggg aaaacctgtg
     2941 gtgactggta caaattacca acacaggtgg ttcgaaagcc gctaagcaat atagctagta
     3001 aaaaacgctc tgagtatcga aaacgatacg ctttgttgga ttatatccat gaaactgcta
     3061 tccgctcttt ggatgcatat cctgtaaact cagaacatca ggacttaaat taaggtttga
     3121 tgtattctct gaataaaata ttaatgatga ttttggtagg ggcattcgca ctaaataatg
     3181 aaaaaataca aatccgagtt tattcctgaa tttaagaaaa attatctttc ccctgtttac
     3241 tggtttacat ggttcgtttt gggaatgatt gcaggtattt caatgtttcc cccttcattc
     3301 agagatcctg tcttggccaa aatagggcgt tgggtgggca gattgagcag aaaagctcgt
     3361 cgcagggcga cgattaattt atcgctttgt ttcccggaaa agagtgatac agaacgggaa
     3421 ataattgtcg acaatatgtt tgccacagca ttgcaatcca tagtgatgat ggcagaactg
     3481 gcaattcgtg gtccggaaaa gttccagaaa cgagtgttct ggaaaggcct cgaaattctt
     3541 gaggaaatcc ggcacaataa cagaaatgtg atttttctgg ttccccatgg ctggagcgtg
     3601 gatattcctg caatgttgct ggcagctcag ggggaaaaaa tggccgccat gtttcatcag
     3661 caacgaaatc cagtgattga ttatgtctgg aattctgtac ggcgtaaatt cggggggcgc
     3721 ttacattccc gggaggatgg gataaaacca tttattcagt cagtacgcca gggatactgg
     3781 gggtattacc ttccagatca ggatcatggt cctgaataca gtgaatttgc tgattttttt
     3841 gcgacctata aagcgacatt accaattatt ggacgtctga tgaacatcag tcaggctatg
     3901 attataccgc ttttcccggt ttatgatgaa aaaaaacatt tcctgactat tgaagttcgg
     3961 ccaccaatgg atgcatgcat tgccagcgcg gacaataaaa tgattgcccg acaaatgaac
     4021 aaaacagtgg aaattttggt ggggtcacat ccggaacagt atatctgggt tttaaaattg
     4081 ttaaaaacgc gcaaatcaaa cgaagcggac ccgtaccctt gaaattataa agtacaggta
     4141 tttccactag ttgtttctta caggttacca atcgaaacac atcccccttc cgtgtgttgc
     4201 ccgtttggca cggaggactg tgtagatgca gttcgttaaa tttaatttat gcctaaatag
     4261 ctgcgcttaa taccgctaca cttttgccag
//
LOCUS       DAJTVJ010000232         4271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000232 DAJTVJ010000000
VERSION     DAJTVJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.234"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..460)
                     /locus_tag="OO448_004231"
     CDS             complement(<1..460)
                     /locus_tag="OO448_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6339263.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQ"
     gene            complement(453..863)
                     /locus_tag="OO448_004232"
     CDS             complement(453..863)
                     /locus_tag="OO448_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6339264.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(869..1402)
                     /locus_tag="OO448_004233"
     CDS             complement(869..1402)
                     /locus_tag="OO448_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6339265.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1380..2462)
                     /locus_tag="OO448_004234"
     CDS             complement(1380..2462)
                     /locus_tag="OO448_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6339266.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLGENILSCDTERSIRERFSEYQVSGQRPGND
                     DDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETT
                     YTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPAD
                     AYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2459..3850)
                     /locus_tag="OO448_004235"
     CDS             complement(2459..3850)
                     /locus_tag="OO448_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6339267.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(3897..4103)
                     /locus_tag="OO448_004236"
     CDS             complement(3897..4103)
                     /locus_tag="OO448_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339268.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1025 a         1246 c         1010 g          990 t
ORIGIN      
        1 gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct gccacaacag
       61 gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag gtcacctgat
      121 tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg ccatcccagc
      181 gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt ctggcgtgcc
      241 ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga taatcgatat
      301 acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc gcatccatac
      361 gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc atagaaataa
      421 gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact ccagatatca
      481 tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc aagtgccagc
      541 gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc atcctccacc
      601 atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt atttgtcagc
      661 ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac cgcaggccag
      721 gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg ccgccatgta
      781 aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt cgacacgggt
      841 cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc gtgtcagtga
      901 tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc cgcatttgct
      961 gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat tcgactggca
     1021 tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc ccgtccacca
     1081 caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc gccacttccc
     1141 cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc acaccatgag
     1201 aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg gcagatgtaa
     1261 aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc attttcaacc
     1321 ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg gaaatcagat
     1381 tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt cctggcggta
     1441 acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt cgtgccccgg
     1501 tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa attattcagc
     1561 gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc ctgccgccag
     1621 ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc ttcaaattca
     1681 caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa caaagggcga
     1741 taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt ggcctcacca
     1801 aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa ccgctctctg
     1861 atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac cagcgccgtc
     1921 gccgctttac ctgttccggg ttttcccaga accagtcgcc cgcattcatc gtcataagcc
     1981 agcgtctgaa cctgccccag taaccggtta agacaatcgg caacggtttc gccgtgttcc
     2041 ggctgggcat caatcaccgc tgtctgcggc acgccagcat caacaacggt gatgccaaat
     2101 ggcgcagcca gttcactgac tattctgagc aggttttttc cgctctgctg gagtggcaaa
     2161 gcagagcagt caaccagatc ggctgttttg cttcgcccga caattcccat gctgacgctg
     2221 ctggcgtcat aacgaagcgg tagtgcctcc acatatccgg tgagcacagg ctcatccccg
     2281 ataagcactt caaccagctc accatttttt atccggggtt gataatcccg gcttccgggc
     2341 cagcgggtgg taatggcaac attaaaatcc cgggcaatac ggttaatgcc cgcactgata
     2401 cggacggatg tccagccgcc ccattcgcga ccggaaaccc gaagtaaaac ggtatcattc
     2461 atctgacggg tacccttaat gccctgaccg gaacaaagcc cggatgagag atggcatttc
     2521 gatccaggat atcagtttca cgggacgcat cgtcgtacca tgacgcagcc agaacaagcg
     2581 caggcagaac ttccgctggc gttcgctctg cggtttcctc cgtctgaacc agacgcgcct
     2641 gaatatcccg gttcagttct gtacgtaatg acgtcagctg aaaaaacagc ctgtcatccg
     2701 ccgtacgcct gagctcctgt tcaatcgccg cgttcagcga tttgcgtatg atggtgagat
     2761 tttcccgcgt gggtggttgc gctctctcat cctgtcctgt gctggctgcc acgctgtcaa
     2821 gtgccggatg agaaacgtga ataatatcgg actgacgttc agtggaacca ccaacagcca
     2881 caaccgcctg ctgatttttc accaggcttc cgggttgtgg cagtgaagtg accgcccttg
     2941 tggcttcgct gattgccgtc gtccggatga cagcagccac aaggtttgtc tgctgttttt
     3001 gcctgataac cgatgcggaa tctgtgggcc acaccgcacg cggtgccagt cctgggtcca
     3061 gcgtaatgcc ggacattgtc gttatggact gcaccagatc ctgtgtgtta tccaccagcc
     3121 gggttccggc ccgccaggta tcctgcaacg tatgcacaaa atcactggca accgacggcg
     3181 gcatcagaat gacggataaa tcgccctgca gtagtctcgc tccggcagaa acagccgaat
     3241 taaccatttt gaaagctgtc tgaacggttc ccagcatatc ggtcgtccgg gcaataacat
     3301 cgttctgaat aaagtccggt attcccgcga gatcaaaatc gccgaacatg tcttcaatca
     3361 actcatccag gaatacggac gactcctcca gttttctggc ggttgcagct ccggcgaccg
     3421 gaaatgacag ttccccgctc tcaacaaact gaaatgaaac ccggcacata cggccttcag
     3481 tgccggagtg agaaacggtc acctgcccgt caatacagcc ctgcatttca ccgaactgcg
     3541 gatggatcag cgtccccggc ccggcggttt caatcgctgt aatcagcctg tcgcgctgct
     3601 ctgcgtaatc atcaccaacg agatacgcat taatcgtcag ccgtcgcgtg gcacgcccga
     3661 gatcttccgt gtacggtttg tcacggtttg ggtattcatg gacctgaacg cggcgtccga
     3721 acgaaccctc gtcgctctcc accgaaaacg gaacgccacg aaatgaagcg tcgtaaagat
     3781 tatcgcgcca attgatacct gaagatgcag aaaagagtga agaaagagaa ggtaaagaag
     3841 gtaagtccat actgtctcca ctataaaaaa accgccaatt tctggcggct tatatattaa
     3901 tcatcattaa tatctttcat attcaatttg caattttcgt aggtcactcc cgactcgcca
     3961 ttaacattaa ctccagctct gatattcccg ttttctttta catctacaaa aaactggcct
     4021 cttggcatct tgaacgcaaa tgtaaaacca acagtatctg aatcaaaatc atcatcttta
     4081 tatattggga tcaaaaatgt catcgtcgct ggtgatgcat tgtttgcaaa atcaaataca
     4141 cctatacttt tgccgaattc ataagaagct tttctcaata taatcgcacc gctggcagaa
     4201 ggggatgggc acgatatcct cagttgtctg aatccatctg tatccaagtc aacgtcatta
     4261 tctgcagatg t
//
LOCUS       DAJTVJ010000233         4247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000233 DAJTVJ010000000
VERSION     DAJTVJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.236"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="OO448_004237"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="OO448_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6339269.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OO448_004238"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OO448_004238"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR6339270.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OO448_004239"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OO448_004239"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCR6339271.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4086..>4247
                     /locus_tag="OO448_004240"
     CDS             4086..>4247
                     /locus_tag="OO448_004240"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6339272.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGI"
BASE COUNT         1048 a         1079 c          980 g         1140 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca gtcgaagtat
     4201 ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaatt
//
LOCUS       DAJTVJ010000234         4192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000234 DAJTVJ010000000
VERSION     DAJTVJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.237"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO448_004241"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO448_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6339273.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="OO448_004242"
     tRNA            complement(330..405)
                     /locus_tag="OO448_004242"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="OO448_004243"
     tRNA            complement(412..486)
                     /locus_tag="OO448_004243"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="OO448_004244"
     tRNA            complement(603..687)
                     /locus_tag="OO448_004244"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="OO448_004245"
     tRNA            complement(696..771)
                     /locus_tag="OO448_004245"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="OO448_004246"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="OO448_004246"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6339274.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO448_004247"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO448_004247"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6339275.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO448_004248"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO448_004248"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6339276.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1042 c          936 g         1134 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAJTVJ010000235         4188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000235 DAJTVJ010000000
VERSION     DAJTVJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.238"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(8..1069)
                     /locus_tag="OO448_004249"
     CDS             complement(8..1069)
                     /locus_tag="OO448_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6339277.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="OO448_004250"
     CDS             complement(1066..2463)
                     /locus_tag="OO448_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6339278.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="OO448_004251"
     CDS             2618..3616
                     /locus_tag="OO448_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6339279.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4188)
                     /gene="ompG"
                     /locus_tag="OO448_004252"
     CDS             complement(3727..>4188)
                     /gene="ompG"
                     /locus_tag="OO448_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6339280.1"
                     /translation="FNGWLSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNY
                     YLERGFNMDDSRNNGEFSTQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIERE
                     GHDFNRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1019 a         1140 c         1035 g          994 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaattgatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaa
//
LOCUS       DAJTVJ010000236         4177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000236 DAJTVJ010000000
VERSION     DAJTVJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.239"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..326
                     /locus_tag="OO448_004253"
     CDS             <1..326
                     /locus_tag="OO448_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6339281.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            553..1818
                     /locus_tag="OO448_004254"
     CDS             553..1818
                     /locus_tag="OO448_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6339282.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIRGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1830..2696
                     /gene="ydiB"
                     /locus_tag="OO448_004255"
     CDS             1830..2696
                     /gene="ydiB"
                     /locus_tag="OO448_004255"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6339283.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2727..3485
                     /gene="aroD"
                     /locus_tag="OO448_004256"
     CDS             2727..3485
                     /gene="aroD"
                     /locus_tag="OO448_004256"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6339284.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3629..>4177
                     /locus_tag="OO448_004257"
     CDS             3629..>4177
                     /locus_tag="OO448_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339285.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDI"
BASE COUNT         1092 a          934 c         1004 g         1147 t
ORIGIN      
        1 cattttatgt ggtgataata tttgcttttg tcattggttt tacctctgct ggaggtgttg
       61 tgcaaattgg cctgacgtta atggctgaac gtttccctta cgctaaaggt aaagctacag
      121 ggatctatta cagtgcgggc agtattgcga cctttactat tccgttgatt acggctcatc
      181 tgtcccaaag aagtattgcc gatattatgt ggttcgatac cgccatcgct gccatcggtt
      241 ttttactggc actgtttatc ggcttacgca gccgcaaaaa aacgcggcat cactcgctaa
      301 aggaaaatgt cgctccgggt gggtaatgca atattctttt caggtcatgc aagatcttac
      361 ggataaataa ctctttctgc gctaactaag gaaaatcgcg atcaaaaaca aactatgaca
      421 tgcaatattc ctggaaacat aaactttatg ccatgtaccc agggaaaatc atcttcagta
      481 tagtaattat gtaaaccgtc ggagaacaat acgtacggta acgaaattat ctttcagcaa
      541 ggagctgtga aaatgtctca aaataaggct ttcagcacgc catttatcct ggctgttctt
      601 tgtatttact tcagctattt cctgcacggc attagtgtta ttacgcttgc ccaaaatatg
      661 acatctctgg cagaaaagtt ttccactgac aacgcgggca ttgcctattt aatttccggt
      721 atcggtttgg ggcgattgat cagtatttta ttcttcggtg tgatctccga taagtttggt
      781 cgtcgggcgg tgatattaat ggcagtaata atgtatctgc tattcttctt tggtattccc
      841 gcttgcccga atttaactct cgcctacggt ctggcagtgt gcgtaggtat cgctaactca
      901 gcgctggata cgggtggcta ccccgcgctc atggaatgct ttccgaaagc ctctggttcg
      961 gcggtcatac tggttaaagc gatggtgtca tttgggcaaa tgttctaccc aatgctggtg
     1021 agctatatgc tgctcaataa tatctggtac ggctatgggc tgattattcg gggtattctg
     1081 tttgtactga tcacgctgat gctgttgaaa agcaaattcc ccagccagtt ggtggacgcc
     1141 agcgtagcta atgaattacc gcaaatgaac agcaaaccgt tagtctggct ggaaggtgtt
     1201 tcatcggtac tgttcggtgt agccgcattc tcgacctttt atgtgattgt ggtgtggatg
     1261 cccaaatatg cgatggcttt tgctggtatg tcagaagctg aggcattaaa aaccatctct
     1321 tattacagta tgggctcgtt ggtctgtgtc tttatttttg ccgcactact gaaaaaaatg
     1381 gtccggccca tctgggctaa tgtatttaac tctgcgctgg caacaataac agctgccatt
     1441 atctacctgt acccttctcc actggtgtgt aatgccggcg cctttgttat cggtttctca
     1501 gcagctggcg gcattttaca gctcggtgtt tcggtcatgt cagagttttt ccccaaaagc
     1561 aaagccaaag tcaccagtat ttatatgatg atgggtggac tggctaactt tgttattccg
     1621 ctgattaccg gttatctgtc gaacatcggc ctgcaatata tcattgttct cgattttact
     1681 ttcgcgctgc tggccctgat taccgcaatt attgttttta gccgctatta ccgcgttttt
     1741 attattcctg agaatgatgt gcggtttggc gaacgcaaat ttagcacccg gttaaacaca
     1801 attaagcata gaggttaaag gagttaatta tggatgttac cgcaaaatac gaattgattg
     1861 ggttgatggc ttatcctatc cgccacagtt tatcgcctga aatgcagaat aaagccttag
     1921 aaaaagcggg attgccattt acctatatgg ccttcgaagt ggataacgat agctttcccg
     1981 gagcaattga aggattaaaa gccctcaaaa tgcgcggaac tggtgtgtcg atgccgaaca
     2041 aacaactggc gtgtgaatat gttgatgaat taacgccagc agccaaactg gtgggtgcca
     2101 tcaacaccat cgttaatgat gatggctatc tgcgtggcta taacaccgac ggcacgggcc
     2161 atattcgcgc cattaaagag agcggttttg atatcaaagg caaaacgatg gtgctgttag
     2221 gggccggagg tgcctcaaca gcgattggcg cgcagggggc aattgaaggt ttaaaagaaa
     2281 ttaaactctt taaccgtcgg gatgagttct tcgataaagc cctcgccttc gcgcagcggg
     2341 ttaacgaaaa caccgattgt gtcgtcacgg ttaccgatct cgccgatcag caagcctttg
     2401 ctgaagtcct ggcttccgcc gacattttaa ccaatggcac aaaagtaggt atgaaccccc
     2461 ttgagaatga atcattggtt aatgatatca gtctgttaca tccgggactg ctggtcactg
     2521 aatgcgtgta taacccgcat atgacgaagt tattgcagca ggcacaacaa gcaggttgca
     2581 aaacgattga tggatacggc atgttgttgt ggcaaggggc tgaacagttc acgttatgga
     2641 ctggcaaaga tttccctctg gaatatgtta aacaggtcat ggggttcggt gcctgacagg
     2701 ctgaccgcgt gcagaaaggg taaaaaatga aaaccgtaac tgtaaaagat ctcgtcattg
     2761 gtgcgggcgc acctaaaatc atcgtctcgc tgatggcgaa agatatcgcc cgcgtgaaat
     2821 ccgaagctct cgcctatcgt gaagcggact ttgatattct ggaatggcgt gtggaccact
     2881 ttgccgacct ctccaatgtg gagtctgtca tggcggcggc aaaaattctc cgtgaaacca
     2941 tgccagaaaa accgctgctg tttaccttcc gcagtgccaa agaaggcggc gagcaggcga
     3001 tttccaccga ggcttatatt gctctcaatc gtgcagccat cgacagcggc ctggttgata
     3061 tgatcgatct ggagttattt accggcgatg atcaggttaa agaaaccgtc gcctacgccc
     3121 acgcgcatga tgtgaaagtt gtcatgtcca accatgactt ccataaaacg ccggaagccg
     3181 aagaaatcat tgcccgtctg cgcaaaatgc agtccttcga cgccgatatt cctaagattg
     3241 cgctgatgcc gcaaagtacc agcgatgtgc tgacgttgct taccgcgacc ctggagatgc
     3301 aggagcagta tgccgatcgt ccaatcatca cgatgtcgat ggcaaaaact ggcgtaattt
     3361 ctcgtctggc tggtgaagta tttggctcgg cggcaacttt tggtgcggta aaaaaagcct
     3421 ctgcaccagg gcaaatctcg gtaaatgatt tacgcacggt attaactatt ttacaccagg
     3481 cataagcaat aatatttcgg cgggaacacc ctccccgccg aacttaaaaa atatatttaa
     3541 ccgtatttaa taaaaatatt tcgtgagtct ctgtgcgcta attctccatc tggcgtaggg
     3601 aaaatcacat ctgaatcagg aattaacaat gaaacctgta aaaccacctc gtattaatgg
     3661 acgagtgccg gtcctgtcgg cacaggaagc ggtgaattat attcccgacg aagcaacact
     3721 ttgtgtgtta ggcgctggcg gcggtattct ggaagccacc acgttaatta cagctcttgc
     3781 tgataaatat aaacagactc aaacgccacg taatttatcg attattagtc cgacagggct
     3841 tggcgatcgc gccgaccgtg gtattagtcc tctggcgcaa gaaggtctgg tgaaatgggc
     3901 attatgtggt cactggggac aatcgccgcg tatttctgaa ctcgcagaac aaaataaaat
     3961 tattgcttat aactatccac aaggtgtact tacacaaacc ttacgcgccg ccgcagccca
     4021 ccagcctggt attattagtg atattggcat cgggacattt gtcgatccac gccagcaagg
     4081 cggcaaactg aatgaagtca cgaaagaaga cctgattaaa ctggtcgagt ttgataacaa
     4141 agaatatctc tattacaaag cgattgcgcc agatatc
//
LOCUS       DAJTVJ010000237         4142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000237 DAJTVJ010000000
VERSION     DAJTVJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.240"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            65..172
                     /locus_tag="OO448_004258"
     CDS             65..172
                     /locus_tag="OO448_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6339286.1"
                     /translation="MTLAELGMAFWHDLAALVIAGILASMIVNWLNKRK"
     gene            548..655
                     /locus_tag="OO448_004259"
     CDS             548..655
                     /locus_tag="OO448_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6339287.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(671..2350)
                     /gene="bcsG"
                     /locus_tag="OO448_004260"
     CDS             complement(671..2350)
                     /gene="bcsG"
                     /locus_tag="OO448_004260"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6339288.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLIRAQIAITAA"
     gene            complement(2347..2538)
                     /gene="bcsF"
                     /locus_tag="OO448_004261"
     CDS             complement(2347..2538)
                     /gene="bcsF"
                     /locus_tag="OO448_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6339289.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2535..4106)
                     /gene="bcsE"
                     /locus_tag="OO448_004262"
     CDS             complement(2535..4106)
                     /gene="bcsE"
                     /locus_tag="OO448_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6339290.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1068 a         1029 c         1074 g          971 t
ORIGIN      
        1 attcttgaac ctgaacaact caagaatagc cgcgaaacgt tgtcattaca acacaggcgg
       61 ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctctggtcat
      121 tgctggcatt cttgccagta tgatcgtgaa ctggctgaac aagcggaagt aacgtgtcat
      181 gcgggcgtca ggctgccgta atggcaattt gcgcccggac caggccgcag gggtgaaact
      241 ctgcggcctt tttcgtaaga aaaatgcaac gattgcacgc ggataatatt ctgcggaatg
      301 cgcagcacag agtgaagacg tgacggattc cggaacagaa aaacagtcgt agaatcagcg
      361 gcaagccgga aaggttccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt
      421 ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa catcacgggg
      481 gctaatcttg aacccaaaca actcaagaat agccgcgaaa cgttggcatt acaacacagg
      541 cggctatatg acgctcgcag aactgggcat ggccttctgg catgatttag cggctccggt
      601 cattgctggc attcttgcca gtatgatcgt gaactggctg aataagcgga agtaacgtgt
      661 catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg gatcaggcga acgtacggtt
      721 tatcctggta ttgaataact actgcatttg agttctcgga gaccggtgct gtttgtggca
      781 acccactggt gagttttttc cagtcaacat tgtcttcggt gaaaatcttg ccatcaagaa
      841 cgcgaaccac cagatcggag atagctagga agctgctcgg ttggtcaatg acaatcggtg
      901 ccccctgatg tggtgccttc atgccgaaga atttcacccc aacggggacg tctgtgatag
      961 acgggctagg gatatcacgt aggccagata cctgcattct gtcgcccttc agcgcgccgc
     1021 cgtgttccgg caccacgacc accatcactt tacgacccga tttctccagt tcagtaaaga
     1081 aggcgtccag ttcatcaaag aatttctgcg cccgcgcttt gtaatccgct gttttgctga
     1141 cccccggata atggttgccg tcatgcagtg gaagcgtgtt gtagaacgtg gcactacggc
     1201 tgtttttatc tttttcggta acgtccagcc agcggttaag cacggcggta tcgtcataaa
     1261 ccggcgaacc atcaaagccc agcaaaataa ccggcagatt tgtttgatcc atcaattcag
     1321 tctgcatgcc gccattttcg cgaacttctt tcaaaaaacc gccgaactgg ccgttatgtc
     1381 ccatcatcag gtgctgggta aagcccagtt tcgaaaggtt atcaaacaga tagcagtcgt
     1441 tatttgccgg ttgatacaga ttagtgtgcg aagtctgccc gcagctggcg cgcagtaaac
     1501 ggatcgccgc cgggccactg taggaggtgg cggagttaaa gttcttgaac tcaatatcga
     1561 aatgcgacca cagtggatgc gacatcaacc cggcggcttc tatatccgac caggaaagcg
     1621 aacagatgtt aatcaccagt agttcaaatg gctgagcatc agcgggcagc gaagacggga
     1681 aggtcgattt acgtttcgcc tccgcgttat agaaattatt cagccaggcg ttaaggttcg
     1741 ccgttgttgg cggtgcagtt tgtgcgggca tatcacccac taccgatgcg ccacccgtcg
     1801 ccgcaacggt tgccgctgcg ttaccacccg tcgttgttac agtggtcgtc ggttgtccgg
     1861 ctggccacaa ggagaaactt ggtcccgcca gggtaagtac gttcagccat agcagtatgg
     1921 caaccacaaa aacggtaatg cgaatccatt gtgacaggaa taaccaggcc actaataaaa
     1981 caaaaatggc cccaatcatc tgccagttaa taaagcgtgt gacaaggtcg attaaataat
     2041 cggtactgaa ccccgccacc tgcgaaccct ggctcattat gctttccggg ccaggcaacc
     2101 aggtgtcatg ccagaacaaa gcaaagccga tcggcagggc aatccagtgg cgcaagcgat
     2161 gcaggctgta gcgcggaatg ggcatcagca gaaacgtggc aaacaccaaa ttgaggagcg
     2221 gatggaagtt aagatatccc gcccacaaca ggccgaactt aaccagaaaa tagaagttcc
     2281 agccggaaag gccgcgccag tattgccaga gggaagaagg catggcggta ttttgcgtaa
     2341 attgagtcat tttttggttg ccctggcttt ttccgtgcgg cgtaacgtcc cggccggttt
     2401 aacataacga ggtttagcaa agaataaacg taaggtgtcg cgaatgcgtc gcaaaaagtg
     2461 ccgcgccaga tagcccagcg ggaaaaatat cagtgcgcaa acgacaataa tttcaatgat
     2521 atcgctgatg gtcatcatga tgagcgctcc acagcatcat ctaacagtcg catgggttct
     2581 ggtattcgtc gccagtggcg accatcgtgc tcggcattga tgaccggttt agaactttgc
     2641 gttaaaggca gcggcatgcc ccattgttct ggggcaagca agcgcatctg caccagctcg
     2701 gcactgattt gatcatcttc aaaccagacc atacggtttg agaaaatgtc gccagtaggc
     2761 aatgggaaaa tatgattcaa cgcggtatcc agatcgttaa tccgacagaa tgagagaaac
     2821 agcaccagcc gattaccgcc aatggtcatg atatcgccgg tacggttagg gcgacacagc
     2881 gtcagggctt gttcaacgcg gatacccggt accggacgta gggcaaccag aacgccttta
     2941 ccgtgggcag gtaatagagg gttattcatc atgttgttga cggcattaca gaacacatcc
     3001 cacttctgga aaccacgcag tttgagcggc tgggtcattg acagcaaggt agtgatatct
     3061 tccggcacat agcgactaaa cttctgccct tgcacgcttt cgatcatcgt cagacaacgg
     3121 gagagtggcg cattccacgg aataaccata tttgcaccgc aggccaataa caaacgttca
     3181 tcggtggcgc gcaggctagc ggtattttcc cgcacgagga ttttcatcgc actaccgcgc
     3241 tggcgacgca gggtatgaat gctgcgggcc agtggctcga tttgcgcatt ttgctgtaaa
     3301 gaaaagacca ccgtcgccgc ctgagcggta cgggcttcat tgaacaggac ttcgttattg
     3361 ttgaacagtt gccagtgttc cgatagcggc ggcgcacctt ccagtacagc aacattactc
     3421 agaatgcgtt tttcgtcgct gcgtggttgg atctccgcct cttcgctttg aactaatgtc
     3481 cagataccat tttgttgctg aacgctaagc tgctgacggg cgctgacccc tttttcgttg
     3541 caccagaagg caatatccag caaatgttgg tcaccctgaa aacgcaaact ggcaagacca
     3601 aaaagtgaac ggtattcctc aagcaacaat gaaaattgtt tatcgttatt atttccggga
     3661 ttaattacca aaagcgaaca atggtttaac ctgctccatt tattcatttt atccaaccat
     3721 gagcgaagcc gctcggcagg aatgttttgc catgcgttat ttgcgcaaac aagaataaaa
     3781 aggtaattat ggggatcaat agaacactgc aaatcacggg ccaaatagta tagacctttt
     3841 tcatgattta gcattgaaaa taattttatt ttttccggcc cttgagaatc atctaattga
     3901 aagattttcg ccggatcgct gtccatgcta atgaccgcga cgtgtgcggt ttcagcctgg
     3961 gatgcaattg tttgattcgc cagactgata gcatcttcat ggcgatcgac gttaaaccac
     4021 cagacgccgc ctgctggcat atgtcgcagc tcatcccata atgatgagat accgatagag
     4081 aatacagggt ccacaatgtc cctcatgtca ccattttgtc tgattatcag tttactagcg
     4141 aa
//
LOCUS       DAJTVJ010000238         4132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000238 DAJTVJ010000000
VERSION     DAJTVJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.241"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            116..529
                     /locus_tag="OO448_004263"
     CDS             116..529
                     /locus_tag="OO448_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6339291.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(574..891)
                     /gene="hspQ"
                     /locus_tag="OO448_004264"
     CDS             complement(574..891)
                     /gene="hspQ"
                     /locus_tag="OO448_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6339292.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(949..2139)
                     /gene="rlmI"
                     /locus_tag="OO448_004265"
                     /pseudo
     CDS             complement(949..2139)
                     /gene="rlmI"
                     /locus_tag="OO448_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2234..2512
                     /gene="yccX"
                     /locus_tag="OO448_004266"
     CDS             2234..2512
                     /gene="yccX"
                     /locus_tag="OO448_004266"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6339293.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2509..2838)
                     /gene="tusE"
                     /locus_tag="OO448_004267"
     CDS             complement(2509..2838)
                     /gene="tusE"
                     /locus_tag="OO448_004267"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6339294.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2929..3588)
                     /gene="yccA"
                     /locus_tag="OO448_004268"
     CDS             complement(2929..3588)
                     /gene="yccA"
                     /locus_tag="OO448_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6339295.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3795..3882)
                     /locus_tag="OO448_004269"
     tRNA            complement(3795..3882)
                     /locus_tag="OO448_004269"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3846..3848),aa:Ser,seq:tga)
BASE COUNT         1101 a         1048 c         1017 g          966 t
ORIGIN      
        1 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
       61 catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
      121 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
      181 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
      241 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
      301 cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta attcagaagc
      361 ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct ggatgcaact
      421 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
      481 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
      541 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
      601 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
      661 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
      721 ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg ccagctcatc
      781 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
      841 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
      901 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
      961 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
     1021 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
     1081 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
     1141 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
     1201 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
     1261 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
     1321 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
     1381 ctaggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
     1441 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
     1501 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
     1561 cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac
     1621 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
     1681 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
     1741 tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
     1801 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
     1861 cagtcagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
     1921 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
     1981 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
     2041 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
     2101 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
     2161 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
     2221 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
     2281 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc
     2341 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
     2401 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct
     2461 cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt
     2521 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
     2581 tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
     2641 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
     2701 cacttcccag tgttccggtg acagcgaaat cccttcgttc tctgcaatca ccactgccag
     2761 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
     2821 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
     2881 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
     2941 ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
     3001 aaccgtggca cgaatatagt tcgtttcacc gccatgaacg atgttgctgg tttcaaacaa
     3061 aatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
     3121 ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
     3181 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
     3241 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
     3301 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
     3361 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
     3421 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
     3481 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
     3541 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
     3601 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
     3661 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
     3721 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
     3781 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
     3841 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
     3901 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
     3961 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
     4021 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
     4081 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca aa
//
LOCUS       DAJTVJ010000239         4117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000239 DAJTVJ010000000
VERSION     DAJTVJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.242"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2877
                     /locus_tag="OO448_004270"
                     /pseudo
     CDS             1..2877
                     /locus_tag="OO448_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            3858..4117
                     /locus_tag="OO448_004271"
                     /pseudo
     CDS             3858..4117
                     /locus_tag="OO448_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1025 a          999 c         1275 g          818 t
ORIGIN      
        1 ggtgacctgg ccggggaaat caccgacgtg acggacggtg ccgggcggga gttccgtctg
       61 gtgctgacca cgcaggcgca gcgtgcggaa gaggcccgca cctcttcgct atcttcttct
      121 gacagttccc gccctctctc agcctcagcg ttccccgaca cactgcccgg taccgaatac
      181 ggccccgaca ggggtatccg cctttcggcg gtgtggttga tgcacgaccc ggcatacccg
      241 gagagcctgc ccgctgcgcc actggtgcgg tacacgtata cggaagccgg tgaactgctg
      301 gcggtatatg accgcagcaa tacgcaggtg cgcgctttca cgtatgacgc gcagcacccg
      361 ggccggatgg tggcgcaccg ttacgcggga aggccggaga tgcgctaccg ctacgacgat
      421 acggggcggg tggtggagca actgaacccg gcagggttaa gctaccgcta tctttatgag
      481 caggaccgca tcaccgtcac cgacagcctg aaccggcgtg aggtgctgca tacagaaggc
      541 ggggccgggc tgaaacgggt ggtgaaaaaa gaactggcgg acggcagcgt cacgcgcagc
      601 gggtatgacg cggcaggaag gctcacggcg cagacggacg cggcgggacg gcggacagag
      661 tacggtctga atgtggtgtc cggcgatatc acggacatca ccacaccgga cgggcgggag
      721 acgaaatttt actataacga cgggaaccag ctgacggcgg tggtgtaccc ggacgggctg
      781 gagagccgcc gggaatatga tgaaccgggc aggctggtat cggagacatc gcgcagcggg
      841 gagacagtac gctaccgcta cgatgacgcg cacagtgagt taccggcgac gacaacggat
      901 gcgacgggca gcacccggca gatgacgtgg agccgctacg ggcagttgct ggcgttcact
      961 gactgctcgg gctaccagac ccgctatgaa tacgaccgct tcggccagat gacggcggtt
     1021 caccgcgagg aaggtatcag cctttaccgc cactatgaca accgtggccg gttaacctcg
     1081 gtgaaagacg cacagggccg tgaaacgcag tatgagtaca acgccgcagg cgacctgact
     1141 gccgttatca ccccggacgg caaccggagc gagacacagt acgatgcgtg gggaaaggcg
     1201 gtcagcacca cgcagggcgg gctgacgcgc agtatggagt acgatgctgc cggacgtgtc
     1261 atcagtctga ctaacgagaa cggcagtcac agcgacttca gctacgatgc gctggaccgg
     1321 ctggtacagc agggcggctt tgacgggcgg acgcaacgtt atcattatga cctgaccgga
     1381 aaactcacgc agagtgagga tgaggggctt gtcaccctct ggcactacga tgcgtcggac
     1441 cgcatcacgc accggacggt gaacggcgac ccggcagagc agtggcagta tgacggccac
     1501 ggctggctga gagaaatcag ccacctgagc gaaggccacc gtgtcgctgt ccactacggc
     1561 tacgacgata agggccgcct gacgggcgaa cggcagacgg tggagaaccc ggagacgggg
     1621 gaactgctgt ggcagcatta gacgaaacac gcctacaacg aacaggggct ggcaaaccgc
     1681 gtcacgccgg acagcctgcc gccggtggag tggctgacct acggcagcgg ttacctggcg
     1741 ggcatgaagc tgggcgggac gccgctggtc gagtatacgc gggacaggct gcaccgtgag
     1801 acggtgcgca ccctcgcacc ggacctggac atccgcatcc cgtatgccac cgacccggcg
     1861 ggaaaccgcc tgccggaccc ggagctacac ccggacagca cgctcacggc gtggccggat
     1921 aaccgcatcg cagaggatgc gcactatgtt taccactatg atgaatacgg cagactgacg
     1981 gagaagacgg accgcatccc gacgggtgtg atacggacgg acgacgagcg gacccaccac
     2041 tacgacagcc agcaccgcct ggtgttccac acgcggatac agcatggcga gccactggtc
     2101 gagagccgct acctctacga cccgctggga cggcgaatgg cgaaacgggt atggcggcgg
     2161 gagcgtgacc tgacggggtg gatgtcgctg tcgcgtaaac cggaggagac atggtacggc
     2221 tgggacggcg acaggctgac gacggtacag accgacacca cacgtatcca gacggtatac
     2281 gagccgggaa gcttcacgcc gctcatccgg gtcgagacag agaacggcga gcgggagaaa
     2341 gcgcagcgcc gcagcctggc ggagaagctg cagcaggaag ggagcgagga cgggcacggc
     2401 gtggtatttc cggctgaact ggtgcggctg ctggacagac tggaggaaga aatccgggca
     2461 gaccgcgtga gcagtgaaag ccgggtgtgg cttgcgcagt gcgggctgac ggtggagcaa
     2521 ctggccagac aggtggagcc ggaatacaca ccagtgcgaa acgttcatct ttaccactgc
     2581 gaccatcggg gccagccgct ggcgctcatc agcgaagacg gcgatacggt gtggagcggg
     2641 gcgtatgatg agtggggcaa ccagctaaat gaggagaacc cgcatcatgt gtatcagcca
     2701 taccgtctgc cggggcagca gtatgatgag gagtcggggc tgtactacaa tcgtcaccgg
     2761 tactatgatc cgttgcagag acggtacatc acccaggacc ccattgggct gaaaggtggg
     2821 tggaatctgt atcagtatcc gctgaatccg gtgagcagga tagatccgtt ggggttattc
     2881 tcatgtgaga gttgtagtca ttttatagat gatataaata ataaactgga taaccttccg
     2941 tttaacccga caaaaatgtt tacagctagt ttaaatactg ctaattcagc aaggctgtat
     3001 gcaagtggca tgacaaaatt cggtataagt gttgctgccg atggaacgat cattgctgcc
     3061 ccagtcggtg cagcttctat ggctttaggg gcgtggaata taaaatctgc gacagctgca
     3121 tataacagag caaatttaca attatcagaa tcacttcagg aaaatggtag agataggtgt
     3181 tggagaaatt tattgggtat tttaccttat ggtgagcagt ttgatgaccc tggtgaacca
     3241 tcaatattag acgtttatgg tgaaaagatt gagaatatca aagattctcc aatgaagttt
     3301 tttgaagagc ttgggacgct ggggttgtaa tgtgtatttt taatgatatt tttatgtttg
     3361 tgtttattgt tagtagtata cttgtaaccg tgcattttgt gtattcttta ttttttaaaa
     3421 gtggaaaggt aaaagataag gtaaaaaaat ggattaaagt aatgtgtgat ttattttggg
     3481 ggataggata gtggtgtgat atggataaat ataaattaca gtggctggca tatttcgacc
     3541 tgacgtcgga gtagataaaa aaacagacag gattggagta tagcaagcgt ctgacaatct
     3601 ctcgggacag tagtatgatg aagaggcggg gttgtactat aaccgtaacc ggtactacga
     3661 tccgctaaag ggcaagtata tgactcaggg aaagatcatc agaagttcac tttttgtact
     3721 aaataattcg cattttatgt ttaaaaattg agatattcct cattacctga agctgttttt
     3781 ttattgctta tacataatca aatactcctt acataattaa ggagaacaaa atggaaatta
     3841 aaaaattgat ggaacatttt ttgaagcaat atggtgattt tgaaaatggc attcctgttc
     3901 acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcat gagtgcttta
     3961 ttaactggat gcgtgactgc cattcttcag atgataaaga cgtcattgca attgatggaa
     4021 aaacgctccg gcactcttat gataagagtc gtcgcagggg agcgattcat gtcattagtg
     4081 cgttctcaac aatgtacagt ctggtcctcg ggcagat
//
LOCUS       DAJTVJ010000240         4079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000240 DAJTVJ010000000
VERSION     DAJTVJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.243"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..787
                     /locus_tag="OO448_004272"
     CDS             <1..787
                     /locus_tag="OO448_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6339296.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            800..1760
                     /locus_tag="OO448_004273"
                     /pseudo
     CDS             800..1760
                     /locus_tag="OO448_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1798..2697)
                     /gene="nhaR"
                     /locus_tag="OO448_004274"
     CDS             complement(1798..2697)
                     /gene="nhaR"
                     /locus_tag="OO448_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6339297.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2763..3929)
                     /gene="nhaA"
                     /locus_tag="OO448_004275"
     CDS             complement(2763..3929)
                     /gene="nhaA"
                     /locus_tag="OO448_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6339298.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1226 a          953 c          861 g         1039 t
ORIGIN      
        1 tattgccaaa acacgtcacc aaattaactt atcgaattcg acctcatttt ccaaagatgg
       61 ctattcctcc aacaatactg gaattactgg catagccggt gaacatgatc agttaaatta
      121 tggtatatat gttaatcagc aacaacaaaa taatgatacc tcgcttggta cgaatttaag
      181 ctggagaact cccatcgcca taatagatgg cagctatagt cattctaaaa acgcctggca
      241 aagtggtgga agtattagta gtggattagt tgtctggccc ggtggtatta atatcactaa
      301 ccagttatcc gatacatttg caattctgga tgcgcctggg ttagaaggtg cacatattaa
      361 tggacaaaaa tacaatcgaa caaacagcaa aggccaggtt gtttacgacc tgataatacc
      421 tcatcgtgaa aaccatctgg tacttgatat agcaaacagt gaaagtgaaa cagaattgca
      481 gggcaatcgt caaattattg caccttaccg tggagcagtt tcttatgtgc agtttacaac
      541 tgaccaacgt aagccctggt atatacaggc actgcgtccc gatggttcgc cattaacctt
      601 tggctatgac gtactggatc tccaggaaaa taatattgga gtcgttggcc agggtagtcg
      661 cctttttatt cgcgtagatg aaattcccac tggaataaaa gttgctctca atgatgaaca
      721 gaacttattc tgtactatta cttttcaaca cgttatcgat gaaaacaaaa catatatatg
      781 ccagtaaaag gagtttacta tgaagtggct tttattaatc accttgagtc tatattcttt
      841 tattgtgcaa agtgctccct gtgcattgac caatgtcggg gaacaacgtg gaacatatat
      901 actcaagcca ctgagtatga aaggtaatct caccgccgaa aaaattgaaa acaccggaaa
      961 tatcattgag tgtgcagact tacaagagac gaccaacgcc attaaagttc attttttaac
     1021 acagaacgct ttaaggttta aagtcggaag tcgtcattat attattaatt tcccctttga
     1081 atctgacggt aaaaaaaata gagcttccag gaaggtcatc ttataattta gaagaaatgc
     1141 tatctgcgat aaatttcacc attccatact ctatcgcatc tgtatcagat agttccaaca
     1201 ccgcagatat ttttccagga actccttttc ctttgttgtt cgaaattgaa gtgagttcat
     1261 gtacagggaa taatactaat tgtaatagcg tccgctttaa ttataattta aatactattt
     1321 tacaggtaaa tttaatgacc tgtggttttg aagatcaatc catcgacact ggacgatttg
     1381 tactgtctcg tatcaataat gaaaattatc agtttcatca tataagattt gactgcatcc
     1441 agggcgagca aggtgaatta gtttttgaac ccagtgatgt tgaatattac tttgaaccag
     1501 tgacgataca ggagtcagga accagcatat taaaaaatga gttagaaaac agtgaggacg
     1561 gtgcgggtca agtaggcttt caaccctcca atgatggtac gaatgaaatc cagtatggta
     1621 aaagcaatta ctactctttt cagcatcccc atgaaggaag taatcagatt cctctattta
     1681 ttcgcccgcg tacatatgga aataatgtct caagcgggca aataatgagc cgagtcaaaa
     1741 tcgtggttat gtacaactaa tctatctccc ccaccttaac tttgggggag gtgccgatta
     1801 acgcgccgct ggactaaaaa gcgcagaata atccgtattg cagattcgct gtaccgccgg
     1861 gtgctgaatc atccgctcag caaaaatagc atggtactct tccatcacat tctcgacgcg
     1921 accaatttct acgacagttt tatcggcata aaagtcatat gcataaagcg ttggggcaac
     1981 gaagattgca ttgtgcatcg caccaaaagc tttcatcaac gcggcatcat caaactcgcc
     2041 gaggatttct acgtttaatc cctgggagtt aaaccagtta agcaatttgc gccctaacat
     2101 tgaacgtcgc ccaggaatca aaagtcgccg ttcttccaga caagccggga acgatttttc
     2161 tggtggtgga tttgtacacc agaaactcac gccacattcg ccaattctca cggagaacag
     2221 gccttcctgc tgcgtagagt ctatcggaca gtcagaaatg atcatatcca gtttatgctg
     2281 acttaattgc tccagcagca tttcgtgggt ggattcgaag cagcgaagat gaatgggctc
     2341 gccttctacc actgcggcgt taagtacgct actgaccagg cgtttggaaa gtgcatcagc
     2401 cacgccaacg tcaaacaata aattggattc tttgcgatag ttcacaatat ccagcatttc
     2461 ctggcttaag gtgaacattt tatcggcata gcgatagacc agttctccca gctcgctggg
     2521 ttcgagacca cgtcccttgc gtttaaataa tttgccttgc aggcgctctt ccagcgctcg
     2581 aatctgtccg gtaatggttt gtggagttaa ataaagcgcc tccgctgcgc caaccacgga
     2641 accttcttta tagacatgcc agaagtaata caagtggttg taattgatat gagacatgct
     2701 catttctctc cctgataaca atgaaaaggg agccgtttat ggctccccgg taaaccgtcc
     2761 tgtcaaactg atggacgcaa acgaacgcgt aaccagctgt atccaattac cgccgaagag
     2821 atagaaccga ccaggatacc gagtttcgcc cagttaatca gttctggatc tacgctacca
     2881 aaggccaggc tggcaataaa gatagacata gtaaaaccga taccgcacag gatccccacc
     2941 gccataattt gctgataagt cgttccctca ggcagatgcg ccagtttcaa acgcagcgcc
     3001 aaccagcaga acagactaat ccccagcggt ttgccaatca gcaagccagc gatgatcccc
     3061 aatggcagaa tggaggtcaa gccatccagc gtgacgcctt gcagtgaaac gccagcatta
     3121 gcaaatgcaa acagcggtaa aatcagatac gccacccacg ggtgcaatac atgctccagt
     3181 cgcttcgctg gagaacgccc atgcttctct ttcaaaggaa taaagaagcc gacaattacc
     3241 cccgccaggg ttgcgtgaac ccccgatttc aacaccgcag tccacaacac cacgccaaca
     3301 agaatataga cgcccgtgcg gcgtacacca cacagattca ataccgcgag taccgcaatt
     3361 gctacagccg cgacgccaag agaggccatc gataagtcat tagtgtagaa caatgcgatg
     3421 ataatgatgg ccccaagatc gtcgataata gccagagcca tcaaaaagat cttcagtgct
     3481 aacggaacac gacttcccaa cagcgccagc acaccaagtg caaaggcaat gtcagtagcc
     3541 gccgggatcg cccacccttc gcgggtaatc ggatcggcat agttaaaggc cagatagagt
     3601 aatgccggaa caatcatccc accaatagcg gcgataaccg gaaatgcggc ctggcgtaag
     3661 ctggctagcg atccttgcat cagttcacgt ttaacttcca gaccgactaa caggaaaaat
     3721 accgccatca gcgcgtcatt tatccataac agcatgtttt tgttgatttc gagtgaacca
     3781 acccggagct gaaccggcgt ctccagaaag tcgtgatacc atccactggt tgcgccgctg
     3841 ttggccatca tcatcgccag gatagcggca atgataagaa taatgcctcc cgaggcatca
     3901 ctgctaaaga atcgatgcag atgtttcact ttttatttct ctttcaggtg aatagatcgt
     3961 ttttactatt ttaccccgca caaatcatcg ttaaaatcag attatagtgt aggaataact
     4021 cgttttttac gagcaattaa aggatttgcg ccatcagcgg tgagatgagt ttttgacgg
//
LOCUS       DAJTVJ010000241         4029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000241 DAJTVJ010000000
VERSION     DAJTVJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.244"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            4..1150
                     /locus_tag="OO448_004276"
                     /pseudo
     CDS             4..1150
                     /locus_tag="OO448_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1444..2685
                     /gene="agp"
                     /locus_tag="OO448_004277"
     CDS             1444..2685
                     /gene="agp"
                     /locus_tag="OO448_004277"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6339299.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2723..2950)
                     /locus_tag="OO448_004278"
     CDS             complement(2723..2950)
                     /locus_tag="OO448_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6339300.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(2971..3576)
                     /gene="wrbA"
                     /locus_tag="OO448_004279"
     CDS             complement(2971..3576)
                     /gene="wrbA"
                     /locus_tag="OO448_004279"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6339301.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3628..>4029
                     /locus_tag="OO448_004280"
     CDS             3628..>4029
                     /locus_tag="OO448_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6339302.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVKL"
BASE COUNT         1135 a          861 c          952 g         1081 t
ORIGIN      
        1 tatatgggta gtaatataca tggaattagt tgcactgcaa ataattattt gaaacaggcc
       61 tggaacgata taaaaaatga gtacgaaaaa aatcaaacat attcaatcac gctttttgaa
      121 aacacactgg tgtgttttat gcggttatac aatgaactca gacgtaaagt aaatgaagag
      181 gatactccat gtctggaatg tgaatcacta gaaaaagaat ttgaggaaat gcagaatgat
      241 aatgatctat cattatttat gagaacattg cgtactaatg atacacaaat ttattcaggg
      301 gtttcaggag gtattacata tactatacaa tatgttcaaa atgttgatat tgttagagtg
      361 tccttgccgg gcagaggttc agagtctatc acagatttta aaggttatta ttggtatggc
      421 tttatggaat atattgaaaa cattactgcg tgtgatgata ttttttctga gtattgttta
      481 gatgatgaaa atatgagtat ccagccagaa cagataaata cgccgggaat atctgattta
      541 gatactggtt ttaaaagaac atgagtattc atgggcgctg ggcggccata agacgcaaaa
      601 gcaagtatac gaagagttta ttaaaattgt tgataaacaa acgatcgagg ctcttgttga
      661 tacggcattc tataatctca gggaagatgt aaagacgtta tttggcgtta atctacaatc
      721 tgacaacaaa attcaggggc agggtggttt tttatcatgg agttttttgt cttttaaaaa
      781 agaatttatt gatagttgct tgaatgacaa aaaatgcata ctgcatttac ccgaatttat
      841 atttaatgat aacaaggcac gccttgtttt agacaccgac ccagagcaga aggttaatga
      901 ggtgaaaaat ttcctcactg ctctttcaga tagcatttgc tcattattta ttgtttacag
      961 caatgtggca tcaatctcct tagggaatga atccttttca acagatgacg accttgagta
     1021 tgggtattta ataaacactg gcaatcatta tgacgtttac ctccctcctg aactttttgc
     1081 tcaggcttac gagttaaaca ataaggaaag gaatgcgcaa attgattttt taactcgtta
     1141 tgcaatttaa tgacaaaggc atgtgctaaa aaccattgtt attagcctca cttctcttat
     1201 tattggtact attgcctctg tattagtaac gccgctgata ttctgtttat ccacaggtgc
     1261 aattatcagc ggcgtacgcg aggcaggggc taatcaggca tagtttgcgt caaaccttgc
     1321 ctgtttttga agatgtatat agaaaaacag gcgttcaaca agccattttg cgaacctgtt
     1381 cccggaaaaa agtcatattt ctgtcacact ctttaatgat tgataacaaa agaggtgcca
     1441 ggaatgaaca aaacgctaat cgccgcaact gtggcaggga tagttttact cgcttcaaat
     1501 gctcaggcac aaaccgtacc ggaaggctat cagctacagc aagtgctcat gatgagccgc
     1561 cataacttac gtgcgccgct ggcgaacaat ggcagtgtgc tggagcagtc gacgccgaat
     1621 aaatggccag aatgggacgt ccccggtggg caactcacca ccaaaggtgg cgtgctcgaa
     1681 gtgtatatgg gccattacat gcgtgaatgg ctggcagagc aggggatggt gaaatcgggg
     1741 gaatgcccgc cgccggacac cgtttatgcc tatgccaata gtctgcaacg taccgttgcg
     1801 accgcacagt tctttattac cggcgcattc ccggggtgtg atattcctgt gcatcaccag
     1861 gaaaaaatgg gcactatgga cccaaccttt aacccggtga tcaccgatga ttccgccgca
     1921 ttcagtgaac aggcggtggc ggcaatggag aaagagctca gcaaactcca gcttaccgac
     1981 agctaccagc tactggaaaa aatcgttaac tataaagatt cccctgcctg taaagagaaa
     2041 caacagtgtt cgctggtgga tggcaaaaat acctttagcg ccaagtatca acaagaacca
     2101 ggtgtttccg ggccgctgaa agtcggcaac tcgctggtag atgcgtttac tttgcaatat
     2161 tacgaaggtt ttccgatgga tcaggtggcc tggggagaaa tcaaatctga ccagcagtgg
     2221 aaggtgttgt cgaagcttaa aaacggctac caggacagcc tgtttacctc accggaagtg
     2281 gcgcgcaatg ttgcgaaacc gctggtcagt tatatcgaca aagctctggt caccgatcgc
     2341 accagcgcac cgaaaattac agtgttggtt gggcacgact ccaacattgc ctctctgtta
     2401 acggcgctgg atttcaaacc gtatcagttg catgaccaga acgaacgcac gccgattggc
     2461 ggcaaaatcg ttttccagcg ttggcatgac agcaaagcca atcgcgattt gatgaaaatt
     2521 gaatatgtgt atcagagtgc ggaacagtta cgtaatgccg atgcgttaac cctgcaggca
     2581 cctgctcagc gtgtgacgct ggaattaagc ggttgcccga tagatgccaa cggtttctgc
     2641 ccgatggata aatttgatag cgtgttgaat gaagcggtga aataacagaa aactcccccg
     2701 cgagaagcgg gggagtcgct ggttaaacgt ttttacgttc gatggtctgt tcgccccaaa
     2761 aaagcgaatc tttatcggcc ttagcaaagg ctttgactaa cacttcatcg ctaccttctt
     2821 cccaaatctt ttctgccatt ttttcgtcgt acccggcgac ttcgaaaatg gcctcggcta
     2881 tttccggcga cgtattgcgc agagatgccc attcaccgac gtgatgagct ttcgcttctt
     2941 gagttggcat gcgtatcctc ctgttgaaga ttagccgtta agtttaactg ccagacctgc
     3001 gacatattcc ccttgataac gagcaataga cagttcttcc tggcttggct ggcgtgagcc
     3061 gtcaccgcct gcgatggtgg ttgcgccgta cggcgtaccg ccgcgaacct gtgaaacgtc
     3121 aaataattcc tgcgctgcgt agccaatggg gacaatcacc atgccgtgat gcgcaagggt
     3181 cgtccaggtt gaagtaatag tttgttcctg accgccgcca gtaccggtgg aactaaagac
     3241 gctcgccagt tttccgtata gtgcgccgga agcccacagg ccgcccgtct ggtcgaggaa
     3301 ggtacgcatt tgaccggaca tgttgccaaa gcgggtaggt gtaccaaaaa taatggcgtc
     3361 gtaatcggcc agttcttgcg gggttgcaac cggtgcagtt tgcgttttac cgcctgcttt
     3421 ttcaaataat tgcggcggca tggtttccgg tacacgctta acgacaactt ctgcgccatc
     3481 gactttgctt gcaccctcag cgactgcgcg tgccatcgtt tcaatatgtc cgtacatgga
     3541 ataataaagc actagtattc ttgccttaac actcatatca ttgttctcct gttcaatgaa
     3601 ccacttagat aagcatagtc cgaagatatg aagctgctag tgacatacat caggtggagt
     3661 actaaagagc aagactcagg agactcctta agacggcaaa caaacctcat tgatgctttc
     3721 tactctaagc acaaaaacga ttattacctt cttcctgctc acagatatgt tgataagggt
     3781 aaatctggtt ttcaccagca acataagaat caaggttctg acttcagacg tatgtttgaa
     3841 aatgttatgt ctggggctat tccagaaggt tccttgattg ttgtagagaa ctttgaccgc
     3901 ttcagtaggg ctgatatcga cacagctata gatgatgtca ggcagatact gaggaagggg
     3961 gtctctatac tgactctggg tgatggtgaa ctctacgaca agtcagcctt aacagaccct
     4021 gtgaagttg
//
LOCUS       DAJTVJ010000242         4024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000242 DAJTVJ010000000
VERSION     DAJTVJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.245"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            172..507
                     /locus_tag="OO448_004281"
     CDS             172..507
                     /locus_tag="OO448_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6339303.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            500..982
                     /locus_tag="OO448_004282"
     CDS             500..982
                     /locus_tag="OO448_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339304.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1016..1096)
                     /locus_tag="OO448_004283"
     CDS             complement(1016..1096)
                     /locus_tag="OO448_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339305.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1132..1556)
                     /locus_tag="OO448_004284"
                     /pseudo
     CDS             complement(1132..1556)
                     /locus_tag="OO448_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1986..2476
                     /locus_tag="OO448_004285"
                     /pseudo
     CDS             1986..2476
                     /locus_tag="OO448_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2701..3192
                     /locus_tag="OO448_004286"
     CDS             2701..3192
                     /locus_tag="OO448_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6339306.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3527..3985
                     /locus_tag="OO448_004287"
     CDS             3527..3985
                     /locus_tag="OO448_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6339307.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT         1505 a          649 c          648 g         1222 t
ORIGIN      
        1 aagatccata atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat
       61 acacacaatg ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc
      121 gcaaagaatt tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc
      181 gcatttgtca taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca
      241 tttttcaagg attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa
      301 gatacaattg atgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg
      361 attaactgta aaaaatatcc gtggttatac ttccctcttg caaaatcctc accggggatg
      421 attgttatgg catgcaataa aaactataac caacatgaag tggcagactg tttaaccctg
      481 tcttaccgtg aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt
      541 tatcagtggg tttattctct tcactgtcta ttcttacact gcagaaaaaa tgatatataa
      601 cgaaacatgc actgcaaatt gggtaatttt taatgaccag ggacgagcaa atcttaccat
      661 cgactttatg tataacaaaa aaaataaaac aggaactgtt gcgttgagtg gaacctggca
      721 acaaggtaac agagaaagta aatcaatacg gcgaaatatt gaatacacat ggattgaaaa
      781 ctatgacaca gcccatttaa catcaaagaa agttaataag tttgagatta tggatcaagt
      841 tgatgatgat agacttgcac aacttattcc tgatttttat gtctttccag aaaaaagtgt
      901 aagctataat atactaaagc aaggtaagca tgcttttatt ttgagcattg gtaacagagc
      961 aataatgcat tgtgcaaggt aaactaaaat agataacaaa aaagatggaa cctcgttaat
     1021 cataaaacaa taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt
     1081 gatcaacatc tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca
     1141 tctggtgcaa atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg
     1201 tacagtcgat gtaaacaata cagaaagagg attattaaga accagtttac tcaggaatca
     1261 atagaggaaa ggactaacgt ttctttaaaa gaattgattt catcatctgt taaactaaac
     1321 tcatcattga cagatcgtga gatataactg tttttaactt tactctttac gttgacttta
     1381 ttgacagaat taacattcac atatcttgaa tttaatgtcc atgatgttgt ttgcgtgaga
     1441 acattctcag cattaaggaa tttttttaac ggaatccttg gtttcttttt cgagtccgaa
     1501 tttacaatat catgcatata gaccatttca tgaatctgcg aaaactcaat gtccattgta
     1561 tacctcacat ttttaccgtg actcgatgtt actgttcaat aatcaccttc catcaatact
     1621 aaaattaata cccctaatgt gccgataaca aatatagtca ttctacgtaa cgtctccata
     1681 aggtgataac cttgatcaaa tgaatttcga atataataaa ggaaatcttt tgagatgttt
     1741 atcacgaata aagctattta tgttaccaat atcatttcga gtgcgcacac tatctatctg
     1801 ttttttcgag atataaaaaa caacgtgtaa gttatgtaaa ttgccgatgc tactgagtaa
     1861 cattatggta aaaaaagtaa aaatgagcga tttttgatag aataaagcat gagaaaaggt
     1921 gatatttgac attatcagaa gctgcgaatt cggattttgc tctaatcaaa tgagagaaat
     1981 gtaaaatgaa gccacgaaat attaataata gcctaccact gcaaccatta gttcctgatc
     2041 aggagaacaa aaataagaaa aatgaagaga aatccgttaa tccagttaaa atcacaatgg
     2101 ggtctggttt aaattatatt gaacaagaat ctcttggagg aaaatatcta acacatgatt
     2161 tgtcaataaa gatagcggat atttctgaag agataattca gcaagcaata ttatctgcta
     2221 tgagcatata taaattttcg ataacagatg atttaatgag tatggctgta aatgaactca
     2281 taaaactgac caaaatagag aataatgtag acctgaataa attcactact atatgcacag
     2341 acgttctatc ccccctcgtc accagacata ataaagaaaa aaaacaaacg acattctacc
     2401 cttctcaaaa atcccctttt taattttcat tgaaaaaatg gcaattgata ctatgaatcc
     2461 aggtcataca cattaaggca tttatgtaat gtcgtaatta agataactaa taaggtgaat
     2521 attagtaaaa caactaatga tgttattagt ttgtagtgaa cagtactttt accaataatg
     2581 aaaaatatac cataagcaat gcaagcttaa aactaataat ttaaaaaaga taaatgtaaa
     2641 aatattaatg agtcaatcaa taataattta gcatcacgag acacatcaca gaggaatatt
     2701 atgagcacag aaacaattga aatattcaat aatagtgatg aatgggcaaa tcaactaaaa
     2761 cacgcattat cgaaaggaga aaatctggca ttactacatg gtttaactcc tgatatcctt
     2821 gatagaatat atgcatatgc attcgactac catgaaaagg gtaatataac ggacgcagaa
     2881 atttattata aatttttgtg catttatgcg ttcgaaaatc atgagtatct aaaagatttt
     2941 gcatcagtat gtcagccaaa aaagaaatat caacaagcat atgaccttta caaactaagt
     3001 tacaattact ccccgtatga tgactattca gttatttatc gtatgggtca atgtcagatt
     3061 ggggctaaaa atatcgataa cgcaatgcaa tgtttctatc acattattaa caattgtgag
     3121 gatgatagtg ttaagagtaa agcgcaggca tatattgaac tcttaaacga taattcagaa
     3181 gataatggct aagcattaca atcatttggt taaatttgct ggttatagtg cagttaacta
     3241 ctataaccag cacctcttat gttttcttaa atcagcatct taaaacagtt aagagcatac
     3301 tataaatcat tatttttcaa tgcgttagag aacaatctac tttataaaaa ttgaataagg
     3361 ctatttcaga ttacatcact gaattcctaa gataaattag tgaacacaac catagatatg
     3421 tcgaatgtat tttcctgtag cattaatgac acataaatat gaatagccat aatttctatt
     3481 gcttaagcag gatgcaagaa accaattttt tcatagaggt taactaatgg acttagaaaa
     3541 taaattctca tatcattttc ttgagggatt aacgctcacg gaagatggaa ttcttactca
     3601 aggaaatgag caagtttata ttccacagaa agagttaggt gtattaatag tattacttga
     3661 atccgctggt catgttgtac tgaaagatat gatcatcgaa tcagtatgga aaaatattat
     3721 tgttagtgac gagtccctga caagatgtat ctattctttg cgctgcattt ttgaaaaaat
     3781 tggctatgat cgttgcatag aaacaatcta ccggaaaggt tatcgtttca gcgggcaggt
     3841 tttcaaaact aaaattaatg aagataatac ttcagactat tccatagcta tattcccttt
     3901 cactacttca ttgaatacac tggatccatt aatacttaat caggaattag tgcaaatcat
     3961 ttcaaataaa aaaatcgatg gttagactgg ccccctgaat ctccagacaa ccagtatcac
     4021 ttaa
//
LOCUS       DAJTVJ010000243         3999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000243 DAJTVJ010000000
VERSION     DAJTVJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.246"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="OO448_004288"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="OO448_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6339308.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="OO448_004289"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="OO448_004289"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6339309.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="OO448_004290"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="OO448_004290"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6339310.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="OO448_004291"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="OO448_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6339311.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          954 a         1130 c          957 g          958 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaa
//
LOCUS       DAJTVJ010000244         3961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000244 DAJTVJ010000000
VERSION     DAJTVJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.247"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO448_004292"
     CDS             complement(<1..242)
                     /locus_tag="OO448_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6339312.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO448_004293"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO448_004293"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6339313.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="OO448_004294"
     CDS             2255..3904
                     /locus_tag="OO448_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6339314.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1032 a         1001 c          928 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 g
//
LOCUS       DAJTVJ010000245         3915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000245 DAJTVJ010000000
VERSION     DAJTVJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.249"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..301
                     /locus_tag="OO448_004295"
     CDS             <1..301
                     /locus_tag="OO448_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339315.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="OO448_004296"
     CDS             390..2258
                     /locus_tag="OO448_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCR6339316.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..>3915
                     /locus_tag="OO448_004297"
     CDS             2295..>3915
                     /locus_tag="OO448_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6339317.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YALMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTYADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRNMENKLHDA"
BASE COUNT         1230 a          721 c          766 g         1198 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtcgg tgatggcggc gctggccccg gtgaggaaca tatagaacga ttaacacgta
     3721 tacgcaatat tgatggctta cctcatgttg atttcagccg tgttgataag ttcttcactt
     3781 acgccgacgc ctttcgcgaa agcttgccca ttatttcagg tgaactttat ttcgaggctc
     3841 atcaagggtg ctttacatca gagtcagcaa ctaaagctca taatcgaaat atggaaaata
     3901 aattacatga tgctg
//
LOCUS       DAJTVJ010000246         3910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000246 DAJTVJ010000000
VERSION     DAJTVJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.250"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..3153
                     /locus_tag="OO448_004298"
     CDS             <1..3153
                     /locus_tag="OO448_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6339318.1"
                     /translation="DRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDI
                     TIKGKTTSQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQC
                     YPNTALVGVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYS
                     NNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNS
                     YLTTQRKAWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPF
                     RYSFSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQ
                     AHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQ
                     TRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVW
                     GLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVT
                     PPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTE
                     TTYRFRQLALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVP
                     KLAVYDPTVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRS
                     VNTVGKSAFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRT
                     SITNVSNEITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAG
                     IGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPT
                     ITSGGNPPAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEG
                     DLVKTVGKPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNI
                     YSSCRLIVKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTA
                     SISDLLVVVMKKATAGITIS"
     gene            3221..3820
                     /locus_tag="OO448_004299"
     CDS             3221..3820
                     /locus_tag="OO448_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6339319.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          915 a          961 c         1205 g          829 t
ORIGIN      
        1 gaccgtctgc gcctgacctt cggtgtgcag gcactggtgg aaaccacctc aaagggtgac
       61 cggaatccgt cggaagtccg cctgctggtt cagatacagc gtaacggtgg ctgggtgacg
      121 gaaaaagaca tcaccattaa gggcaaaacc acttcgcagt atctggcctc ggtggtggtg
      181 ggtaacctgc cgccgcgccc gtttaatatc cggatgcgca ggatgacacc ggacagcacc
      241 acagaccagc tgcagaacaa aacgctctgg tcgtcgtaca ccgaaatcat cgatgtgaaa
      301 cagtgctacc cgaacacggc gctggtcggc gtgcaggtgg attcagagca gttcggtaac
      361 cagcaggtga gtcgcaatta tcatcttcgc gggcgcattc tgcaggtgcc gtcgaactat
      421 aacccgcaga cgcggcaata cagcggtatc tgggacggaa cgcttaagcc agcatacagc
      481 aacaacatgg cctggtgtct gtgggacatg ctcactcatc cgcgctacgg catggggaaa
      541 cgtcttggtg cggcagatgt ggacaaatgg gcgctgtatg tcatcggcca gtactgcgac
      601 cagtcagtga cggacggatt tggcggcacg gagccgcgca tcacctgtaa ctcttacctg
      661 accacacagc gtaaggcgtg ggatgttctc agtgatttct gctcggcgat gcgctgtatg
      721 ccggtatgga acggacagat gctgacgttc gtgcaggacc gaccgtcgga taaggtgtgg
      781 acctataacc gcagtaatgt ggtgatgccg gacgatggcg cgccgttccg ctacagcttc
      841 agcgccctga aggaccgcca taatgccgtt gaggtgaact ggattgaccc agataacggc
      901 tgggagacgg cgacagagct tgtggaggat acgcaggcca ttgcccgtta cggtcgtaat
      961 gtcacgaaga tggatgcctt tggctgtacc agccgggggc aggcacaccg agccgggctg
     1021 tggctgatta aaacggaact gctggaaacg cagaccgtgg acttcagcgt gggcgcagaa
     1081 gggcttcgcc atgtaccggg tgatgtcatt gaaatctgcg atgatgatta tgccggtatc
     1141 cgcaccggtg gtcgcgtact ggcggtgaac agccagaccc ggacgctgac gctcgaccgt
     1201 gaaatcacgc tgccgtcctc cggtaccacg ctgataagcc tggttgacgg aagtggcaat
     1261 ccggtcagcg tggaggtcca gtccgtcacc gacggcgtga aggtgaaagt gagccgggtt
     1321 cctgacggcg ttgcagaata cagcgtgtgg gggctgaagc tgccgacgct gcgccagcgt
     1381 ctgttccgct gcgtgagtat ccgtgagaac gacgacggca cgtatgccat caccgccgtg
     1441 cagcatgtgc cggaaaaaga ggccatcgtg gataacgggg cacactttga cggtgaccgg
     1501 cgcggcacgg tgaatggtgt cacgccgcca gcggtgcagc atctgaccgc cgaagtcacc
     1561 gcagacagcg gggaatatca ggtgctggcg cgatgggaca cgccgaaggt ggtgaagggt
     1621 gtgagcttcc tgcttcgcct gaccgtggca gcggacgacg gcagtgagcg gctggtcagc
     1681 acagccagga cgacggaaac cacataccgc ttcaggcagc tggcgctggg gaattacagt
     1741 ctgacagtcc gggcggtaaa tgcccggggg cagcagggcg atccggcgtc ggtatcgttc
     1801 cggattgcgg caccggcagc gcctgtcact attgaactga taccggggta ttttcagata
     1861 acggcggtcc cgaaactggc tgtatatgac ccgacggtgc agtttgagtt ctggttttcg
     1921 gaaaagcgga ttgcggatat caggcaggtt gaaaccagcg cccgctatct tggcacggcg
     1981 ctgtactgga tagccgccag tatcaatatc aggccgggcc atgattatta tttttacgtt
     2041 cgcagtgtga acaccgttgg caaatcggca tttgtggagg ccatcggtcg ggcgagcgat
     2101 gatgcagaag gttatctgaa tttttataaa gggttgatca ataaaacgca tctcggcaag
     2161 gagttgtgga cgcagattga taacggtcag cttgcgccgg acctgactga aatcaggacg
     2221 tccattacga atgtcagcaa tgaaatcacg caaaccgtca ataaaaaact ggaaaatcag
     2281 agtgctgcaa tccagcagat acagaaagtt caggttgata caaataataa cctgaacagc
     2341 atgtgggctg tgaagctgca acagatgaag gacggacgcc tttatattgc gggtatcggt
     2401 gccggtattg agaatacgcc agcaggaatg cagagtcagg tgctgctggc ggcagacagg
     2461 attgcgatga ttaatcctgc gaatggcaac acaaagccga tgtttgttgg tcagggcgat
     2521 cagatattca tgaacgacgt gttcctgaaa tgcctgacgg ctccgaccat taccagcggc
     2581 ggtaatcctc cggcattttc cctgacacct ggcggacggc tgacggcgaa aaatgccgat
     2641 atcagcggta acgtgaacgc gaactccggg acgctcaaca acgtcacgat taataagaac
     2701 tgtcgggttc tgggaaaatt gtccgcgaac cagattgaag gcgatctcgt taaaacagtg
     2761 ggcaaacctt tcccacggga ctcccgggca ccggagaggt ggccatcagg gaccattacc
     2821 gtcagggttt atgacgatca gccgtttgat cggcaaattg ttattcccgc ggtggcgttt
     2881 cgcggtgcta aacatgagcg gaagaataac aatatttatt cgtcatgccg cctgatagtg
     2941 aagaaaaacg gtgctgaaat ttataaccga acgaccctgg ataatacgct gatatatacg
     3001 ggtgttattg atatgcctgc cggtcacggt cacatgacgc tggagttttc tgtatcggca
     3061 tggctggtaa atggctggta tcccacagca agtatcagcg atttgctggt tgtggtgatg
     3121 aagaaagcca ctgcaggcat cacgattagc tgaattttat aacccagata cgggcaccag
     3181 aaatggtgcc ttttttattg cagaaaagcg agaggtaatt atgcgtaaag tttgtgcagc
     3241 cattttgtcc gcagccatct gtctggccgt atccggtgcg cctgcatggg cgtctgaaca
     3301 tcagtccacg ctgagcgccg ggtatcttca gacccatact gatatgccag gcagcgatga
     3361 cctgaagggc attaacgtga aataccgtta tgagtttacg gacacgctgg ggctgattac
     3421 gtcattcagt tatgccaacg ctgaagatga gcaaaaaacg cattacagcg atacccgctg
     3481 gcatgaggat tccgtgcgta accgctggtt cagcgtgatg gcggggccgt ctgtgcgcgt
     3541 gaatgaatgg ttcagcgcgt atgcgatggc gggcgtggct tacagccgtg tgtcgacctt
     3601 ctccggggat tatctccgcg taactgacaa caaggggaaa acgcacgatg tgctgaccgg
     3661 aagtgatggc ggtcgccaca gcaacacgtc tctggcgtgg ggggctggcg tgcagtttaa
     3721 cccgaccgaa tccgtggcca ttgaccttgc ttatgaaggt tccggcagtg gtgactggcg
     3781 cactgacggt ttcatcgtgg gtgtcggtta taagttctga ttagccaggt aacacagtgt
     3841 tatgactgcc cgctggttca ggcgggcttt ttgtggggtg aatatggcag taaagatttc
     3901 aggtgtactg
//
LOCUS       DAJTVJ010000247         3908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000247 DAJTVJ010000000
VERSION     DAJTVJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.251"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            24..590
                     /locus_tag="OO448_004300"
     CDS             24..590
                     /locus_tag="OO448_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6339320.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="OO448_004301"
     CDS             594..1169
                     /locus_tag="OO448_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6339321.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="OO448_004302"
     CDS             complement(1211..2263)
                     /locus_tag="OO448_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6339322.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="OO448_004303"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="OO448_004303"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6339323.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3777
                     /locus_tag="OO448_004304"
     CDS             3580..3777
                     /locus_tag="OO448_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6339324.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          987 c          951 g          999 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAJTVJ010000248         3878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000248 DAJTVJ010000000
VERSION     DAJTVJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.252"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            452..2104
                     /locus_tag="OO448_004305"
     CDS             452..2104
                     /locus_tag="OO448_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6339325.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2115..2285)
                     /locus_tag="OO448_004306"
                     /pseudo
     CDS             complement(2115..2285)
                     /locus_tag="OO448_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2418..2537)
                     /locus_tag="OO448_004307"
                     /pseudo
     CDS             complement(2418..2537)
                     /locus_tag="OO448_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2611..3045)
                     /locus_tag="OO448_004308"
     CDS             complement(2611..3045)
                     /locus_tag="OO448_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR6339326.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3222..3878)
                     /locus_tag="OO448_004309"
                     /pseudo
     CDS             complement(3222..3878)
                     /locus_tag="OO448_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847473.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1078 a          880 c          898 g         1022 t
ORIGIN      
        1 gtggactggt ataataatcg acgattgctg gaaaggctgg gccacatccc tccggcagaa
       61 gcagaaaaag cttattatgc ttctatcgga aacgatgatc tggcagcctg agttcacaga
      121 taaaacattc tccaggaaac ttggggcggt tcacgtcagt atcagaccat attaattgct
      181 gaaactgata aacatcaaac cttatagaag ggtgaatgct gaaagggggc tatggtgatc
      241 gacgtgatga caacgacagg taagaccgtg acctgataaa cctgaattgt gtcctagaca
      301 taaagtccgt gaagggaata aggctcaggt cagcgaatct tatagtggcc cgcagctctg
      361 actgcaataa ggccggatac agagcagcaa ctactcgtcg atgtcagaac aacggaagcc
      421 ttgcaaacgg ggatgcgttc atacaggtac aatgataccg ttaaaaaaaa acattacgct
      481 aataatgttc accctgtcat tactgacagg aaatcctgct atagcgtatg aaacagataa
      541 agtatataaa ataaccgttc tgcatacaaa tgaccaccat ggacactttt ggcgtaacaa
      601 tcatggtgag tacgggttat cctcccaaaa aactctggtg gacaatatcc ggcagaaagt
      661 aataaataat ggcggaagtg ttctattatt atctggtggt gatattaaca ccggtgtacc
      721 tgaatcagac cttcagaagg ctgaacccga tattcgtgga atgaatctaa ttggttatga
      781 tgcaatggcc gtcggtaatc atgaatttga taatcctcta aatatattac gtcagcagga
      841 aaaatgggcc acatttcctt tcctttctgc taacatatat caaaaaagta cagggaggcg
      901 tttattcagt ccatggaaaa ttttcatccg tcagaatttg aaaattgcag tcattggctt
      961 gacgacagat gatactgcga aaacaggaaa ttctgaatat tttactgata ttgagttccg
     1021 tcaacctgct gctgaggcga ggagtgtcat tgatgaactt aatcagcagg aaaagccaga
     1081 tattattatt gcggcaacac atatgggtca ctatgacaat ggcgagtcag gttcgaatgc
     1141 tcctggtgat gttgaaatgg ctcgcagttt gccgactggc tcattagcaa tgattgtggg
     1201 aggacattca caagctcctg tctgcatggc cagcgataat aaaaaacagt ggaattatat
     1261 tccggggaca acctgtgtac cagataaaca aaatggcatc tggattgtac aagcgcatga
     1321 gtggggaaaa tatgtcggtc aggctgattt tgaattctgt aacggtacaa tgaaactggt
     1381 taattatcag ttacacccag ttaatctcaa aatgagaatt accagagaag atggaaaaac
     1441 ggaattttct ttttacaccc ccgaaattac tgaagatcca cagatgttat ccttactgac
     1501 accattccag aataaaggca aagcacaact ggatgtgaag gttggggttg tgaacggacg
     1561 tctggaaggg gacagaagta aagttcgttt tgttcagacc agtatgggcc atctgattct
     1621 gagcgctctg acagaacgta ttgacgcaga ttttgctgtt gtcagtggtg gggaaattcg
     1681 agattccata gagagtggta acatcacata caaagatata ctgaaagttc agccatttgg
     1741 taatacggta gtaagtattg atttgacggg gaaagaagta gctgactacc tagctacagt
     1801 ggcacagatg aaaccggatt ccggagccta cccacaattt ttgaatacca gctttgtagt
     1861 caaaaaagga aaaatcgaaa tgctaaaaat aaaaggtaaa tcggttgacc ttaacaaaaa
     1921 atatcgtatg acaacattta gctttaatgc tactggtggg gatggctatc cacgtattga
     1981 taatagaccc ggatacataa ataccggatt tattgatgct gaagtactta tcgaatatat
     2041 ccgggaacat tcaccgctgg atgctgcttc atatgaacca aaaggtgaag tcagttggca
     2101 gtgataacgt aattgacgat catcacgtgt tttttgctga cagtcgctgt gactttgtga
     2161 aagtagtctc tgcggatgtt tgcgatatgg gaatgcagtt gctgtatttt gtgtttctgc
     2221 ttctgccagt tgttgctgaa tttaaccttg cggcttaact gatgttggag cgctgccagc
     2281 ttcttcgtca atttcgctga ttaaagttta ttcattatgg catgatctgc gtaagcggct
     2341 cgtcagaacc gtattgatat ttactaagag ctcagatcaa ctttccaggg caacagatcg
     2401 cgtacccggt ttgccggtca gtcctggata tgctcaatga cgtaacgcag ccatttttct
     2461 ggctccacat tgttcagacg gcatgtgccg atcagcgagt acaacaccgc cgcatgttcg
     2521 ccaccgctat cggaaccccg ccggagagag tttcactctc cggctgctca cggaaccgta
     2581 cgtgacactc tcgcgtcata cggctcttga tcactcattc gatgcacgta gtaaatacca
     2641 gtgggcaaat agcttcggat actgtttcct taccagcgcc agctttcgct gagccttccg
     2701 gttcgaaagt ctgtatttag actgcatcca cttaagcaaa cgtgactgca ttgcactcca
     2761 cagtcgatag ctgaagtttc tggaccagaa ctttccatag tactcaatcc agcctctcac
     2821 tatcgcattg tagcgcctcg caaaatccag caaactctca gctgttgagc gatgtatacg
     2881 ccacttcttg attgtttctg ttattttgca cattgctgca tttgatgcac ccggcataca
     2941 ttttctgtac aattcgcctt tgaaattctt cagagtacgc actttgaagt catatccgag
     3001 aaaggagaaa ctcgtagcga cgtttctcct tttaaacgtg tcaatgtagg cgatattcgt
     3061 cttccctgcg ttcaggacca acccgacttc cgagaagcgc accgataacc tgttcttcag
     3121 ccttgttgca tcactcattc gtgaacaatg tactacaagt cgagtcaccc gagggaacct
     3181 ccccctctgg tgctcacgga accggacgtg aacctctcag ctcatccggc tcttctcatt
     3241 gaggcctgtc cccatagtaa cggccagtgt gcaaacaacc tcgtttcact ccgcgcaaca
     3301 cgggccagcc agtggctggc ccgtcgtcgg tggcccctaa gtcgcttgtg tttacggggc
     3361 agccataaca cgagtttgcg atcgatttgc ctcagcgtca gatacagtgc tgatttataa
     3421 aaagcaccgt agtactttat ccagcctctg attttcgcat tgaacatatg cgccagctcg
     3481 tacaaggact tgtcactgcg attctcctca cctcctgacg gattgctttt cctgctctgg
     3541 cactcatcgc cggactgaag ttaacgaagg tcttacccca gcgattcatc gacagacgcg
     3601 gccggaatgt atagcccaga aagtcgaagc tcgtttccgg gtagtccccc cgtcggtcag
     3661 catccttaca gtaaactatt cgtgtttttt gcggatgcag ctgcagcccg cattgcgcaa
     3721 gccgctgcct cagtccactt atcagagcgt cagcctgcca ctggctgtga cagtggcata
     3781 caacatcatc ggcgtacctt tcgaagggaa cgccgggaaa ctgacgttgc atccacatat
     3841 caaaggcata gtgcaggaac agatttgaga gcaggggg
//
LOCUS       DAJTVJ010000249         3854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000249 DAJTVJ010000000
VERSION     DAJTVJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.253"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            116..511
                     /gene="caiF"
                     /locus_tag="OO448_004310"
     CDS             116..511
                     /gene="caiF"
                     /locus_tag="OO448_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6339327.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(720..1310)
                     /gene="caiE"
                     /locus_tag="OO448_004311"
     CDS             complement(720..1310)
                     /gene="caiE"
                     /locus_tag="OO448_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6339328.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="OO448_004312"
     CDS             complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="OO448_004312"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6339329.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2210..3763)
                     /gene="caiC"
                     /locus_tag="OO448_004313"
     CDS             complement(2210..3763)
                     /gene="caiC"
                     /locus_tag="OO448_004313"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6339330.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          909 a         1048 c          941 g          956 t
ORIGIN      
        1 cttatcgacg gtgaagttgc atactatcga tatatccaca attttaatat ggccttgttt
       61 aattgcttca aaacgagtca tagccagact tttaatttgt gaaactggag ttcgtatgtg
      121 tgaaggatat gttgaaaaac cactctactt gttaatcgcc gaatggatga tggctgaaaa
      181 tcggtgggtg atagcaagag agatctctat tcatttcgat attgaacaca gcaaggcggt
      241 taataccctg acttatattc tgtcggaagt cgcagaaata agctgcgcag ttaagatgat
      301 ccctaataag ctggaaggac ggggatgcca gtgccagcga ctggtaaaag tggtcgatat
      361 cgatgagcaa atttacgcgc gcctgcgcaa taacagccgg gataaattag tcggcgtaag
      421 aaagacgccg cgtattcctg ccgttccgct cacggaactt aaccgcgagc agaagtggca
      481 gatgatgttg tcaaagagta tgcgtcgtta atgtcatttc gttgatacca cgcagcctgg
      541 ttgctggatg caatgctggc gcatcttatc cagcctacgg ttgctctcca gccgggttgg
      601 ataaggcgtt tatgccgcat ccgacagcta tcgccggatg cgatgctggc gcatcttatc
      661 cggcctacgg ttgctctcca gcagagttgg ataaggcgtt tacgccgcat ccggcaagat
      721 caccgtttag gcgtcacatc cgtcgtcccc tgcaaacggg ggcgattttc ctccatttgc
      781 ctcagtggct gtgtttcatg taacgatgca tgacagcgcc cgacaagatc ctgatactct
      841 ttggtattca accgtttcca gtgtaactcg tcgtcactaa cactgcgtac agcgcgggct
      901 ggcgtaccca tcaacaactg gcgtttctcg ccgtgaaagc ctgctttgac aaagctcatg
      961 gcggcaacaa tgctctcttc gccaatgacc gcgccatcca taatcacgct gttcatcccg
     1021 accaatgcat cgcgaccaat cacacaacca tgcaggatcg ctccgtgccc gatatggccg
     1081 ttttccccaa cgatggtgtc agtgtcgcag tagccatgca taatgcagcc atcctgaata
     1141 ttggctcccg cttgcacgat caaccgcccg tagtcaccac gcagtgaggc gagtgggccg
     1201 atgtagacac cggctcccac aatcacatcg ccaatcaaga cggcactggg atggacaaac
     1261 gccgtcgggt gaaccaccgg aattaacccc tcaaaggcgt aatagctcac ggttgttaac
     1321 gtcctttcca caccggatcg cgcttctcgg caaacgccag cggcccttca atggcatcct
     1381 ccgaatgcag aaccgacgga tagtgtttca acacgccgct gcgaatatag cgatacgctt
     1441 cttctaccgg catttcgctg gtggtgcgga agatctcttt cagcgccgca atcgccagcg
     1501 gggcgctgtt aaccagctgc tgcgccagtt cgcgggcgtt atccatcagt tccgcctggc
     1561 taaccacgcg gttgactatc ccccaacgca gcgcctcttc tgcgcccatt cgtctgccgg
     1621 tcatcaccat ttcattgacg atggcaggcg gcaggatctt cggtagacgc agcacgccgc
     1681 cgctgtcagg aacgatgccc agcttggctt ccggcagggc gaagctggcg ttatcggcac
     1741 aaacaataaa atctgccgcc agcgccagtt cgaaaccgcc gtcaaaggca tagccgttca
     1801 cagctgcgat aaccggtttg tcgagattga aaatttcggt taatcccgca aatccacccg
     1861 ggccaaagtc agcatccggt gcttcgcctt ctgctgccgc ttttaaatcc cagcccgcgg
     1921 aaaagaactt ctctccggca ccggtaataa tggcgacacg taattgcgga tcgtcacgga
     1981 aatttagaaa tacttcgccc atttcaaagc tggtttttgc atcaatagca ttcgcttttg
     2041 gacgatcaag ggtaatttcc agaattgatc cattgcgggt cagatgtaat gattcactca
     2101 ttccttttct ccatttttgc tttttcaggg acgacaacat ccctgcaaaa aatgcatatt
     2161 gttttatagc gtgattatta gttggcaggg tagttccctg ctgtttcatt tatttcagat
     2221 tctttctaat tattttcccc gagcaattac gtggcagatc ttttctgatc tccagataag
     2281 agggcacttt aaatttcgcc atattttgtt cgcagaagcg gaaaaattcc tcttcgctca
     2341 atgtttcacc ttcattcagc accacaaatg ctttgatggc ttcatcgcga atcgaatctt
     2401 taatacccac aaccacgata tcctgaattt ttggatgggt ggcgatgata ttttccagct
     2461 ccacgcagga gacattctcg ccaccgcgtt tgatcatatt gcagcggcga tcgacgaaat
     2521 aaaaaaagcc ctcttcgtcg cagtatccgg tatcgccggt atgtagccag ccgtcggctt
     2581 ccagcacttt cgcagtggct ttcgggttga gaaaatactc tttgaagatg gttttccctg
     2641 gtacgccttt aatacagatt tcaccgatct cacctgccgg gagcgggcga ttgtgatcgt
     2701 cgcggatctc cgcttcgtag caaaaccccg cccgaccaat cgacggccag cgtcgtttat
     2761 cgccagggcg atcgccgata atgcccacaa tggtttccgt catcccataa gacgtcagca
     2821 agcgaacgcc gaagcgttca caaaacgcat ctttttcctg ctccgacaag ttgagataaa
     2881 acatcacttc ccgcaggcgg tgttgccgat cgttcgctga aggcggctgc gccatcaagg
     2941 tacggatcat catcggaata cattcggtaa cggtggcgcg gtacttctgt acctgtcccc
     3001 agaaggcgcg ggcgctgtat ttctcgacca gcacaaaggt ggccccggca gaaaacgccg
     3061 ccatcgccgc agtacactgg caatcgatat gaaacgcagg cattaccgtc aggtagacgt
     3121 catcgtcacg cagtgcacac tgccaggcgg agtaatatcc agcgaagcgc aggttgtaat
     3181 gggtaatcac cacacctttc ggtcgggagg tggtgccgga ggtgaagaga atttccgccg
     3241 tatcgtcagt cgatagcggc ggtgcatagc acaaggtggc aggttgttga tttttcagtt
     3301 gagtaaacga actcacgcca tcatcagcgg gaagtgccac atctgtcagg caaatgtgcc
     3361 gcaattgagt ggcatcttcc tgctgaatct gttgatacat aggatagaat tgcgcactgg
     3421 tcaccagcag gcacgcctgg ctattttgca ggatccacgc gctttcctcg cgcaacaggc
     3481 gggcgttaat cggcaccata atcgcgccaa tttttgccag cccgaaccag caaaagataa
     3541 attccgggca gttgtcgaga tgtagagcaa ccttgtcgcc tttgcgaatc cccagcgtat
     3601 aaaacaggtt tgtcgtgcgg ttaatctcct gatttaactc aagataacta taccggttaa
     3661 cgactccgcc gctggattca caaatcagcg ccgttttatg accgtaaacg tccgccagat
     3721 cgtcccacat ttgacgtaga tgttgtccgc caatgatatc cattgcacct ctatccattt
     3781 ttgttcgttt gttattgggc gggcgctagt caggcaagcc gactgacgcc acgcgtttag
     3841 tcctcaactt tggc
//
LOCUS       DAJTVJ010000250         3829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000250 DAJTVJ010000000
VERSION     DAJTVJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.254"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..263)
                     /locus_tag="OO448_004314"
     CDS             complement(<1..263)
                     /locus_tag="OO448_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6339331.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            350..757
                     /gene="nudG"
                     /locus_tag="OO448_004315"
     CDS             350..757
                     /gene="nudG"
                     /locus_tag="OO448_004315"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6339332.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(723..995)
                     /locus_tag="OO448_004316"
     CDS             complement(723..995)
                     /locus_tag="OO448_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6339333.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1231..2574
                     /gene="gdhA"
                     /locus_tag="OO448_004317"
     CDS             1231..2574
                     /gene="gdhA"
                     /locus_tag="OO448_004317"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6339334.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2691..3730)
                     /locus_tag="OO448_004318"
                     /pseudo
     CDS             complement(2691..3730)
                     /locus_tag="OO448_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          996 a          931 c          917 g          985 t
ORIGIN      
        1 agagaaatat caagaaagcc gcccgcgtcg gtaagctctc tgcgccgcgc cagcgcaccg
       61 tccagaccat caagcagcct gttcaacaaa atgacgacta gcgccgccag ataccagccc
      121 agcgccagaa acggcagagc cagcacgccg atggcaaatc ccactaatgt taaaccatct
      181 ggcgtaatgc ccggtttatc aagaacccgc acgcactgat gcaacaacgg tttaatccgg
      241 ggatgaagat ggcggtctag cacaggcact ccttaaatat aaagcctttc tgattgagca
      301 acagtgcgga tattatggca tttttcgctt atctgcccgt gtgtaattta tgaaaatgat
      361 tgaagttgtt gccgccatca ttgaacgtga tggcaaaatt ttactcgcgc aacgccccgc
      421 ccagagcgat caggcgggat tatgggagtt tgccgatggt aaagtcgagc tggatgaaag
      481 ccagcagcag gcactggtgc gtgagttaaa cgaagaattg ggcatcgaag caactgtggg
      541 tgagtatgtt gccagccatc agcgagaagt ttcgggccgg attatccatc ttcatgcctg
      601 gcacgtaccc gacttccacg ggacgttaca ggcacatgaa catcaggcgc tggtctggtg
      661 ctcacctgaa gaggcgctgc aatatccgct ggcccctgct gacattccac tattagaggc
      721 gtttatggct ttacgcgccg ccagagcagc ggattagtgc taagggtttt gtcatcccgc
      781 tggcattgca gcagtattcc ttcggcttta attaccgccc cttcagaata attttgatcc
      841 tgataaacgc agcactgagt acagggctgc gctgactgcc cgcctgaact gaacacttct
      901 ggcggtacgt ttacctccac gtccggacga taatgcgggt tagccagtgc aattaatgga
      961 aatgctaata ctacggcgaa caatgctcga ctcacaggga actccttaac gttattgtct
     1021 ctgcaactga taacggtagc cgggtggcaa aactttagcg tctgagttat cgcatttggt
     1081 tatgagatta ctctcgttat taatttgctt tcccgggtca tttttttctt gcttaccgtc
     1141 acattcttga tggtatagtc gaaaactgca aaagcacatg acataaacaa cataagcaca
     1201 atcgtattaa tatataaggg ttttatatct atggatcaga catattctct ggagtcattc
     1261 ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc cgttcgtgaa
     1321 gtaatgacca cactctggcc ttttcttgaa caaaatccaa aatatcgcca gatgtcatta
     1381 ctggagcgtc tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg ggttgatgat
     1441 cgcaaccaga tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc catcggcccg
     1501 tacaaaggcg gtatgcgctt ccatccgtca gttaaccttt ccattctcaa attcctcggc
     1561 tttgaacaaa ccttcaaaaa tgccctgact accctgccga tgggcggtgg taaaggcggc
     1621 agcgatttcg atccgaaagg aaaaagcgaa ggtgaagtga tgcgtttttg ccaggcgctg
     1681 atgaccgagc tttatcgtca tctgggcgcg gataccgacg ttccggcagg tgatatcggc
     1741 gttggtggtc gtgaagtcgg ctttatggcg gggatgatga aaaagctctc caacaatacc
     1801 gcctgcgtct tcaccggtaa gggcctttca tttggcggta gtcttattcg cccggaagct
     1861 accggctacg gtctggttta tttcacagaa gcaatgttaa aacgccacgg tatgggtttt
     1921 gaagggatgc gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc tatcgaaaaa
     1981 gcgatggaat ttggtgctcg tgtgatcact gcgtcagact ccagcggcac tgtagttgat
     2041 gaaagcggat tcacgaaaga gaaactggca cgtcttatcg aaatcaaagc cagccgcgat
     2101 ggtcgagtgg cagattacgc caaagaattt ggtctggtct atctcgaagg ccaacagccg
     2161 tggtctgtac cggttgatat cgccctgcct tgcgccaccc agaacgaact ggatgttgac
     2221 gccgcgcatc agcttatcgc caatggcgtt aaagccgtcg ctgaaggggc aaatatgccg
     2281 accaccatcg aagcgactga actgttccag caggcaggcg tactgtttgc accgggtaaa
     2341 gcggctaatg ctggcggtgt agcaacgtcg ggcctggaaa tggcacaaaa cgctgcgcgc
     2401 ctgggctgga aagccgagaa agttgacgca cgtttgcatc acatcatgct ggatatccac
     2461 catgcctgtg ttgagcatgg tggtgaaggt gagcaaacca actacgtgca gggcgcgaac
     2521 attgccggtt ttgtgaaggt tgccgatgcg atgctggcgc agggtgtgat ttaagttgta
     2581 aatgcctgat ggcgctacgc ttatcaggcc tacaaatggg cacaattcat tgcagttacg
     2641 ctctaatgta ggccgggcaa gcgcagcgcc cccggcaaaa tttcaggcgt ttatgagtat
     2701 ttaacggatg atgctcccca gggaacattt cttatgggcc aacggcattt cttactgtag
     2761 tgctcccaaa attgcttgtc gtaacgataa cacgcttcaa gttcagcatc cgttaacttt
     2821 ctgcgatagc agcagatatg ccagtaaaga aaccccatta gactattttt ttgataatct
     2881 tcttcgcttt cgaacaactc gtgcgccttt cgagaagcaa gcattatata atgccaggcc
     2941 agttcttctt caattgtccc gttttgaaaa gctgtgcttg atatcgaggt catccatgat
     3001 aattccgccg cccatattag cttcgccgag gatttaccgg agcgattatt agcgcaatca
     3061 gagatatagt ctgagggaaa aacagcaaat ttattcaaca aggcgataac ctgctctggg
     3121 gcttcctcca tgtttgcttt aaaggtattg gctccatggt cgccagaaag aaaatgctcc
     3181 attaggcaca ataactttcg ctatcttcga taccccattg attctctaaa gactcgcgtc
     3241 ttttactgat gatatcgatc gagtcaaaag gaagcacatg atattggaag gtatctttac
     3301 caggttcagg ctttcgcggc cagaactcta gcgtttcaga ccattgctta tgatagaatc
     3361 gataaggtgc gatcaattgt agcgcctgta acttctcgat actgagcggc tcaatacctt
     3421 tagcctgata ataatgcagt tgttcttttt ttgctttaaa accggcccga acaataagcc
     3481 ccatcataat taatagataa agaaaagagc atcccgcggt aatcaggcct ctttcattca
     3541 aaccgttgga tgttatcgct gcgaacacaa acattacagc gacaacacat gttaaataaa
     3601 acccccactt acaaagcagc atggccttat tttctttaat catccgttca aaattactat
     3661 taaatatttc ccagccatta aaagaatact tctcgctccc aggatggttt tgtaataaaa
     3721 cttttttcat cacattccct gttattacat actttagagg cgataatttt atccatgcaa
     3781 aaaaaatatc caacgaattt tcttgatccg ctggatattt cagaatatg
//
LOCUS       DAJTVJ010000251         3796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000251 DAJTVJ010000000
VERSION     DAJTVJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.255"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..879
                     /gene="adeQ"
                     /locus_tag="OO448_004319"
                     /gene_synonym="yicO"
     CDS             <1..879
                     /gene="adeQ"
                     /locus_tag="OO448_004319"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6339335.1"
                     /translation="IGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFII
                     TVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLEL
                     AGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTS
                     SVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLM
                     TSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLC
                     VVVVASLFALKIILVD"
     gene            932..1384
                     /locus_tag="OO448_004320"
     CDS             932..1384
                     /locus_tag="OO448_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6339336.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1595..2775
                     /gene="nepI"
                     /locus_tag="OO448_004321"
                     /pseudo
     CDS             1595..2775
                     /gene="nepI"
                     /locus_tag="OO448_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2816..3109)
                     /locus_tag="OO448_004322"
     CDS             complement(2816..3109)
                     /locus_tag="OO448_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339337.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3331..>3796
                     /locus_tag="OO448_004323"
     CDS             3331..>3796
                     /locus_tag="OO448_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HCR6339338.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKL"
BASE COUNT          814 a          848 c         1004 g         1130 t
ORIGIN      
        1 attggattat ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa
       61 gacacgctgg tgatgattgg cgatttaagt tctcacggcg tgttgttagg tattttaggg
      121 ttttttatta taaccgtgtt gtcatcacgt cattttcatg ccgcggtgct ggtttctatt
      181 gtggtgacgt cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc
      241 attccgcctg atattagcgg cgtcattggt gaagtagatt tgagcggcgc gttaacactt
      301 gaactcgccg gtatcatttt ctcctttatg ctgatcaacc tatttgattc atcaggaaca
      361 ttaattggtg taactgataa agcgggctta atagatggta acggtaaatt ccccaatatg
      421 aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg
      481 tctgttactg cctatattga aagtacttct ggtgtggcag tcggtggccg cacggggctg
      541 actgcggttg tggttggcgt tatgttcctg ttggttatgt tcttctcccc gctggtggcg
      601 atggttcctc cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttcg
      661 agcctggcgc gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg
      721 gtgatgatgc cctttacttt ctcgatcacc gaagggattg cactcggctt tatgtcgtac
      781 tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc
      841 gcatctctgt ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga
      901 tatcatgagt tctgataacg caggagaact catgatctgg ataatgctcg ccacgctggc
      961 ggtagtgttt gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg
     1021 tctcagtgat cggctgaata tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg
     1081 aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca
     1141 aaacgccgcg caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa
     1201 cctgctgcaa tggcatcgga ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc
     1261 tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa
     1321 tgcttttcag atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca
     1381 ttaaaaccgc ttcgtcttat cagcctacag accgcgcctg acctgtcggg tggataagac
     1441 gttcacgccg catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag
     1501 caacaccgta aaagttgcaa aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc
     1561 ggcttttccc ctgaaacatg ccacgggtaa caccatgagt gaatttattg ccgaaaaccg
     1621 cggcgcggat gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt
     1681 cgcctgtctg attatcgttg agtttttgcc cgtcagtttg ttgacgccaa tggcccagga
     1741 tttaggcatt tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat
     1801 gtttgccagt ttgtttatta cccagacaat tcaggctact gaccgccgca acgttgttat
     1861 tttgtttgcc gttttgctga cgctctcctg cttgctggtt tcctttgcta actcattcag
     1921 tttgctttta atcggtcgtg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc
     1981 ggcgtcgctg accatgcgtc tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat
     2041 cttcggcgcg gtttctattg cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga
     2101 gcttatcggt tggcgcaatg tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt
     2161 ctggattatc aaatcattgc cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac
     2221 tttccgctta ttacaacgtc cgggtgtgat ggcagggatg atcgccatct tcatgtcttt
     2281 cgccgggcag tttgctttct tcacatatat tcgcccggtg tatatgaacc tggcgggatt
     2341 cagcgtggat ggcttaacgc tggtgctgtt gagttttggc attgccagct ttatcggtac
     2401 gtcgctttcg tcattcattc ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt
     2461 aatactggct gtgagtgcgt tggtgctgac gttgtgggga agcgataaaa tcgttgctac
     2521 cggcgtggcg attatctggg ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg
     2581 gatcacccgc tcactggccg atcaggcaga aaaagccggg tctattcagg tggcggttat
     2641 tcagcttgtg gcgcggcaat cggcggttat gcgctggata atattggtct gacttcgccg
     2701 ttgatgtttt ctggcacatt gatgttgctg actgcattgt tggttactgc aaaggtgaaa
     2761 atgaagaaat cctgatatca ggtaaagaag cgttggtcag tagttgttgc tgttcttaca
     2821 tatccgggca ttctacctta cccaacgctt cccaataatt gctctgatta ttacgttcca
     2881 tcgccacgat ggcctcgcgt atatgttgtt tattgttttc atctgacaac attaactttt
     2941 cccacgcttc atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata
     3001 ccgtggtttt ctcttttgcg gactgcagtg agctaaaagg cgactcggcg tatacgatgc
     3061 ctggcatagc gaaaaaagtg aaaataagca gtagtgtcgt tggtttcatg gattcaacct
     3121 tttacaaatg tgtatcgaat gaagcataag tgtagtttgc tttcatcgct ctcatcatgc
     3181 tgtacgaaac gcgcttgcgt ggtcagtaag aagtgccaga ctttatattc cacttttatt
     3241 cctttttatt cttatcgata gcgtttcgtt ttttaaaccg cagcgacctt accgctatag
     3301 tcaggtaatc attaataaaa ggataaaaaa atgaaactga caacacatca tctacggaca
     3361 ggggccgcat tattgctggc cggaattctg ctggcaggtt gcgaccagag tagcagcgat
     3421 gcaaaacaca ttaaagttgg cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct
     3481 aaaaaggtgg caaaagagaa atatggcctc gatgttgaac tggtaggatt tagcggttcg
     3541 ttgctaccga acgatgcgac aaatcatggc gaacttgatg cgaatgtctt ccagcatcga
     3601 ccctttcttg aacaagataa tcaggcgcat ggttataaac tggtagcgct gggaaatacg
     3661 tttgtcttcc ctatggcggg ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa
     3721 ggcgcgacag tggcgatccc caacgacccg accaaccttg gtcgcgcact tttactgttg
     3781 caaaaagaga agctaa
//
LOCUS       DAJTVJ010000252         3632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000252 DAJTVJ010000000
VERSION     DAJTVJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.256"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..783)
                     /gene="ydcO"
                     /locus_tag="OO448_004324"
     CDS             complement(<1..783)
                     /gene="ydcO"
                     /locus_tag="OO448_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR6339339.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTMTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIV"
     gene            836..1372
                     /gene="sutR"
                     /locus_tag="OO448_004325"
     CDS             836..1372
                     /gene="sutR"
                     /locus_tag="OO448_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR6339340.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1445..3406
                     /gene="rlhA"
                     /locus_tag="OO448_004326"
     CDS             1445..3406
                     /gene="rlhA"
                     /locus_tag="OO448_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR6339341.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3498..>3632)
                     /locus_tag="OO448_004327"
     CDS             complement(3498..>3632)
                     /locus_tag="OO448_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HCR6339342.1"
                     /translation="KNVDHQLRHEADSDELRAVAEESAEGLREHFYWQKSRKPEAGQR
                     "
BASE COUNT          934 a          918 c          932 g          848 t
ORIGIN      
        1 tacaattaat ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg
       61 tgcgttttgc gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc
      121 aaacgaaaaa tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt
      181 gacaacgtca ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc
      241 atagcgcggc gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt
      301 aaattgaccg tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag
      361 cattgccgcc gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat
      421 gccgcagagg actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt
      481 tagtccctgc aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac
      541 aggtacgcga taccataatg tcagagtcat cgtactgacg cccattgcca gccccagcgc
      601 cgtcatccag ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat
      661 tattgccgct gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg
      721 tggaggaata gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag
      781 catttgtccg ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga
      841 aaatctcgct cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag
      901 tctttcgcga ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg
      961 taatgagtcc agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc
     1021 attttcaaca tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca
     1081 gcaggcgatg gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt
     1141 ctcgattcag atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat
     1201 cgaacatgtc gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac
     1261 ccttaattgt ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg
     1321 tggggagcaa accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa
     1381 actatttcgc aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa
     1441 gaatgtgacc gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat
     1501 tgcccgcgaa gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg
     1561 tgcccgtcat aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca
     1621 tcgttatggt gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga
     1681 acccgcgcaa cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca
     1741 ggatatgggg attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg
     1801 cgacattcgt acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt
     1861 gctggcgcga gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac
     1921 cattgaattc tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc
     1981 tcatgcgcaa acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc
     2041 atacacattg aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat
     2101 gaaagataac gatcagactg ccaacctcgg cgcgctgatt gatgctggcg tacgctcctt
     2161 caagattgaa gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg
     2221 ccagatgctg gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg
     2281 tactgaacat ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta
     2341 ttttgtgaat gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct
     2401 gccggtgggt gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc
     2461 actggcaaat ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc
     2521 caatacggtc gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc
     2581 agatttgcac aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca
     2641 gcaggcactg acaaaaacct ccagcgaacg tcgggtggcg gtagacattg aactgggcgg
     2701 ctggcaggaa caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac
     2761 gctggacggg cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga
     2821 tggtctggca aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc
     2881 gggggcgctg tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat
     2941 gctggatgct gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc
     3001 tgcgccggtt tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc
     3061 gcgtgaattt tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga
     3121 agagaagggc gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct
     3181 gtgcccgaaa caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt
     3241 taacggcgat gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat
     3301 tggcaaaatc aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt
     3361 aagtccggat gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct
     3421 ggttactcac aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc
     3481 atccggcatc aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa
     3541 aaatgctcgc gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc
     3601 gcttcatgac gcagctgatg atccacattc tt
//
LOCUS       DAJTVJ010000253         3556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000253 DAJTVJ010000000
VERSION     DAJTVJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.257"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..351
                     /locus_tag="OO448_004328"
     CDS             <1..351
                     /locus_tag="OO448_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6339343.1"
                     /translation="RWYLCPQVRVADIVQLNGNIRPRSRQWWQLFRMVSQWHVDVVIV
                     ELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWL
                     NTTGADQQSPEHRS"
     gene            591..851
                     /gene="repA"
                     /locus_tag="OO448_004329"
     CDS             591..851
                     /gene="repA"
                     /locus_tag="OO448_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6339344.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1075..1149
                     /gene="tap"
                     /locus_tag="OO448_004330"
     CDS             1075..1149
                     /gene="tap"
                     /locus_tag="OO448_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6339345.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1142..1999
                     /gene="repA"
                     /locus_tag="OO448_004331"
     CDS             1142..1999
                     /gene="repA"
                     /locus_tag="OO448_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6339346.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2699..2890
                     /locus_tag="OO448_004332"
     CDS             2699..2890
                     /locus_tag="OO448_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339347.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTAACRLT
                     CRWRGIRIIQAAFNAFAKV"
     gene            2963..3214
                     /locus_tag="OO448_004333"
     CDS             2963..3214
                     /locus_tag="OO448_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6339348.1"
                     /translation="MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            3211..3498
                     /locus_tag="OO448_004334"
     CDS             3211..3498
                     /locus_tag="OO448_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6339349.1"
                     /translation="MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT          940 a          872 c          908 g          836 t
ORIGIN      
        1 cggtggtatc tgtgtccgca ggtacgggtc gcggatatcg tccagctgaa cgggaatatc
       61 cggccacgat cacgccagtg gtggcagtta ttcaggatgg tgtctcagtg gcatgttgat
      121 gtggttatcg ttgagctgcg ttcgttcagt attgttgcag cagtagagct ggatgatgcc
      181 agccatttac gaccggaacg cagacgccgg gatattcttc tggaagaggt tctgaggcag
      241 gctggtattc cgttgctcag aagccacgat gccagaaaac tgctgcagat gaccggagaa
      301 tggctgaata caacaggggc tgatcagcag tcaccggaac atcgtagctg acgccttcgc
      361 gttgcttagt tgtccaaccc cggaaacggg aaaaagcaag ttttcctcgc tcccggcgtt
      421 tccataactg aaaaccatac tatttcacag tttaaatcac attaaacgac agtaatcccc
      481 gttgatttgt gcgccaacac agatcttcgc cacaattctc aagtcgctga tttcaaaaaa
      541 ctgtagtatc ctctgcgaaa cgatccctgt ttgagtattg aggaggcgag atgtcgcaga
      601 cagaaaatgc agtgacttcc tcattgagtc aaaagcggtt tgtgcgcaga ggtaagccta
      661 tgactgactc tgagaaacaa atggccgcag ttgcaagaaa acgtcttaca cacaaagaga
      721 taaaagtttt tgtcaaaaat cctctgaaag atctcatggt tgagtactgc gagagagagg
      781 ggataacaca ggctcagttc gttgagaaaa tcatcaaaga tgaactgcag agactggata
      841 tactaaagta aagactttac tttgtggcgt agcatgctag attactgatc gtttaaggaa
      901 ttttatggct ggccacgcca taaggtggca gggaactggt tctgatgtgg atgtacagga
      961 gccagaaaag caaaaacccc gataatcttc tttaactttg gcgagtcaga aagattaccg
     1021 gggcccactt aaaccgtata gccaacaatc aagctatgcg gggagtatag ttatatgccc
     1081 ggaaaagttc aagacttctt tctgtgctcg ctccttctgc gcattgtaag tgcaggatgg
     1141 tgtgactgat cttcaacaaa cttattaccg ccaggtaaag aacccgaatc cggtgttcac
     1201 accccgtgaa ggtgcccgga cgcttccgtt ctgcgggaaa ctgatggaaa aggcggtggg
     1261 cttcacctcc cgttttgatt tcgccattca tgtggcacat gcccgttcgc ttggtctgcg
     1321 tcggcgcatg ccaccggtac tacgtcgccg ggccattgat gcgctgctgc aggggctgtg
     1381 tttccactat gacccgctgg ccaaccgcgt tcagtgctcc atcaccacgc tggccattga
     1441 gtgcggactg gcgacggagt ctgatgccgg aacactttcc atcacccgtg ccacgcgtgc
     1501 cctgacgttc ctggcagagc tggggctgat tacctaccag acggaatatg acccgcttat
     1561 cggctgcaac attccgaccg acatcacgtt cacaccggcg ctgtttgccg ccctcgatgt
     1621 atcagaggag gcggtggcgt ccgcgcgccg cagtcgtgtg gaatgggaaa acagacagcg
     1681 caagaagcag ggactggata ccctgggtat ggatgaactg atggcgaaag cctggcgttt
     1741 tgtgcgtgag cgtttccgca gttaccagac agagcttaag tcccgtggaa tgaagcgtgc
     1801 ccgtgcgcgt cgtgatgcgg acaggcagcg tcaggatatt gtcaccctgg tgaaacggca
     1861 actgacgcgt gaaatctcgg aagggcgctt cactgccagt cgtgaggcgg taaaacgtga
     1921 agtggagcgt cgtgtgaagg agcgcatgat tctgtcacgt aaccgtaatt acagccggct
     1981 ggccacagct tccccctgaa agtgacctcc tcagaataat ccggcacgcg ccggaggcat
     2041 tcgcacgcct gaagcccgcc agtgcacaaa aaatcagcgc tacatacaaa aaacaacctc
     2101 atcatccacc ttctggtgca tccggtcccc cctgtttttg atacaaaaca cgcctcacag
     2161 acggggaatt ttgcttatcc acattaaact gcaagggact tccccataag gttacaaccg
     2221 ttcatgtcat aaagcgccag ccgttagctt tacagggtgc aatgtatctt ttaaacagct
     2281 gtttatatct cctttaaact acttaattac atccatttaa aaagaaaacc tgttcactgc
     2341 ctgtcctgtg gacagacaga tatgcacctc ccaccgcaag cggcgggccc cgaccgaagc
     2401 cgatttagtt acaacatgca aacataaacc cctaaaaaac ccaagtccga cgcagaactg
     2461 aaaccacaac gcccctcctc ataactgaaa agcgccgctg cccccgcccg aagggcggga
     2521 acagagtcgc ttttaattat gaatgttgta actacatcgt catcgctgtc agtcttctgg
     2581 ctggacgtat cgagtacacg ctcgtaagcg gccctgacgg cccgctaacg cggagatacg
     2641 ccccgacttc gggtaaaccc tcgtcgggac cactccgacc gcgcacagaa gctctgtcat
     2701 ggctgaaaac gggtatggct tagcagggct ggggatgggt aaggtgaaat ctgtcaatca
     2761 gtaccggcta acgccgggct tcggcggttt tactccggta tcacatgtaa ccgcagcgtg
     2821 ccgccttaca tgccgctggc gcggcatcag aataatacaa gcagcattta atgcatttgc
     2881 aaaagtgtga gtaataaaga cttgttaagt ggtcgtttca gggatatcgt taagacttgt
     2941 taaacagtct caaaggtgcg ttatgcctaa catcattctg tctgagacca gtgcaagtgt
     3001 cagtgaactg aaaaaaaatc ctatggctac agtcagtgcc ggagatggtt accctgtcgc
     3061 cattcttaac cggaatcagc cggcattcta ctgtgtgcct gctgaactgt atgaaaggat
     3121 gcttgatgct ctggatgacc aggaactggt gaaactggtt actgaacgca gcaatcagcc
     3181 actgcatgat gtggacctgg acagttattt atgacttata cggtaaaatt cagggatgat
     3241 gcgctcaagg aatggctgaa actggataag accattcaac aacagtttgt gaaaaaacta
     3301 aaaaaatgca gcgaaaatcc tcatataccg tcggcaaaac taagagggct aaaggactgc
     3361 tacaaaatta aactacgtgc atcgggattt cgtctggttt atcaggtgat tgacgatatg
     3421 ttaattattg cggttgttgc ggtaggaaaa cgagaacgta gtaacgttta taatctcgcc
     3481 agtgagagaa tgagatagta acatataaat aacctttccc gcagataact cagaattgtt
     3541 attttctctg tggact
//
LOCUS       DAJTVJ010000254         3547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000254 DAJTVJ010000000
VERSION     DAJTVJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.258"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..143)
                     /locus_tag="OO448_004335"
     CDS             complement(<1..143)
                     /locus_tag="OO448_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339350.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /locus_tag="OO448_004336"
     CDS             complement(189..530)
                     /locus_tag="OO448_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339351.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..890)
                     /locus_tag="OO448_004337"
     CDS             complement(699..890)
                     /locus_tag="OO448_004337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339352.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OO448_004338"
     CDS             complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OO448_004338"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6339353.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2226..>3547)
                     /gene="xylA"
                     /locus_tag="OO448_004339"
     CDS             complement(2226..>3547)
                     /gene="xylA"
                     /locus_tag="OO448_004339"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR6339354.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          925 a          983 c          784 g          855 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 cgtacggtaa tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact
      961 acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac
     1021 atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt
     1081 ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg
     1141 tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg
     1201 ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc
     1261 cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg
     1321 gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata
     1381 gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac
     1441 tgcaccagct gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc
     1501 tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat
     1561 ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac
     1621 atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg
     1681 cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc
     1741 atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt
     1801 aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc
     1861 gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc
     1921 atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag
     1981 agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag
     2041 tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc
     2101 gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat
     2161 cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt
     2221 agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca
     2281 ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac
     2341 atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg
     2401 atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc
     2461 atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga
     2521 cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca
     2581 tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt
     2641 tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct
     2701 atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta
     2761 atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca
     2821 tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg
     2881 attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga
     2941 cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt
     3001 tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg
     3061 aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag
     3121 ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca
     3181 accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc
     3241 acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct
     3301 gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc
     3361 cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa
     3421 cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat
     3481 gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag
     3541 gcttgca
//
LOCUS       DAJTVJ010000255         3545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000255 DAJTVJ010000000
VERSION     DAJTVJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.259"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(100..909)
                     /gene="proC"
                     /locus_tag="OO448_004340"
     CDS             complement(100..909)
                     /gene="proC"
                     /locus_tag="OO448_004340"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6339355.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1029..1487
                     /locus_tag="OO448_004341"
     CDS             1029..1487
                     /locus_tag="OO448_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6339356.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1670..2194
                     /gene="aroL"
                     /locus_tag="OO448_004342"
     CDS             1670..2194
                     /gene="aroL"
                     /locus_tag="OO448_004342"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6339357.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2244..2435
                     /gene="yaiA"
                     /locus_tag="OO448_004343"
     CDS             2244..2435
                     /gene="yaiA"
                     /locus_tag="OO448_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6339358.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2693..3370
                     /locus_tag="OO448_004344"
     CDS             2693..3370
                     /locus_tag="OO448_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6339359.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3442..>3545
                     /locus_tag="OO448_004345"
     CDS             3442..>3545
                     /locus_tag="OO448_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1255 family protein"
                     /protein_id="HCR6339360.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGE"
BASE COUNT          899 a          835 c          931 g          880 t
ORIGIN      
        1 atcgtgagtg gttgaaatcg gcagatttgg cgctttacaa agcaaagaaa gccggacgta
       61 accgcaccga agtggcggcc tgacgtccgg cgaaagtcat caggatttgc tgagtttttc
      121 tgatttttcc atacacttcg tcatcgcttc gatcactgcg gcacggaagc ctttctcttc
      181 cagtacgcgt accgcttcaa tggtggtgcc tcccggtgag cagaccatat ctttcagtgc
      241 ccccggatgt tctcccgttt ccagcaccat ttttgcggaa cccattaccg cctgagcggc
      301 aaatttatac gcctgggcgc gtggcatccc gcccagcacg gcggcgtcgg ccatcgcttc
      361 gataaacata aatacgtagg ctggcgaaga accgctcaca ccgaccaccg ggtggatcat
      421 cggctcagca attacttccg cttcgccaaa gcagcggaaa atattcagca catcagcggt
      481 atcttcgggg gttaccagcg cgtttggcgt tacggaggtc atcccggcat taaccagcgc
      541 gggagtgttc ggcatggcgc ggataatttt ccggtcatgg cccagcgcgc gggcaagctg
      601 gtcgagcgtg acacctgcag caatagaaac gaccagagag tctttattca ggctggaggt
      661 gatttcgcta agcactttaa tcatgatgcc aggtttaacg gcggcaaaaa tgatgtcggc
      721 gatttgcgcc acttcttgcg ccgattctgc ggcgttgatg ccgaactggt catgcagggc
      781 ggcgacttta tccggggagg gggtgtatac ccagatttgc cctggaagca cctgaccgct
      841 ggcaatcaga ccgccgagaa tggcttttcc catattgccg cagccaataa aaccgatttt
      901 cttttccatt gcctcactcc tgccgtgaaa ttcattgttt tgataatcgc tggcagaagc
      961 ataaacagaa ctatgccgga aggcaaaagc gcgacacaat agaggattac ccaacaaagg
     1021 atgactttat gacaatttgg gtggatgccg acgcgtgtcc caatgtaatt aaagagattt
     1081 tgtatcgcgc ggcggaacgt atgcagatgc cgctggtact ggtagcaaac cagagtttac
     1141 gcgtgccgcc atcgcgattt attcgtacgc tgcgcgtcgc ggcaggtttc gacgttgccg
     1201 ataacgaaat tgtccggcag tgtgaagcgg gcgatttggt gatcaccgca gatatacctt
     1261 tggctgctga agccatcgag aaaggcgcag cggcgcttaa tccgcgcggc gaacgttaca
     1321 cgccagcgac cattcgtgag cgcctgacga tgcgcgattt tatggatacc ttacgtgcca
     1381 gtgggatcca gaccggcgga ccagatagcc tttcacaacg tgaccgccag gcctttgccg
     1441 cggagctgga gaagtggtgg ctggaagtgc aacgtagtcg tggctaaatg taatttatta
     1501 tttacacttc attcttgaat atttattggt atagtaaggg atgtattgag attttcactt
     1561 taagtggaat tttttcttta caatcgaaat tgtactagtt tgatggtatg atcgctattc
     1621 tcatgacacc ggctttcgcc gcattgcgac ctattgggga aaacccacga tgacacaacc
     1681 tctttttctg atcgggcctc ggggctgtgg taaaacaacg gtcggaatgg cccttgccga
     1741 ttcgcttaac cgtcggtttg tcgataccga tcagtggttg caatcacagc tcaatatgac
     1801 ggtcgcggag atcgtcgaaa gggaagagtg ggcgggattt cgcgccagag aaacggcggc
     1861 gctggaagcg gtaactgcgc catccaccgt tatcgctaca ggcggcggca ttattctgac
     1921 ggaatttaat cgtcacttca tgcaaaataa cgggatcgtg gtttatttgt gtgcgccagt
     1981 atcagtcctg gttaaccgac tgcaagctgc accggaagaa gatttacggc caaccttaac
     2041 gggaaaaccg ctgagcgaag aagttcagga agtgctggaa gaacgcgatg cgctatatcg
     2101 cgaagttgcg catattatca tcgacgcaac aaacgaaccc agccaggtga tttctgaaat
     2161 tcgtagcgcc ctggcacaga cgatcaattg ttgattttag agcgcctata cttaacgttc
     2221 atcccgtgaa ataaggaaga acgatgccaa cgaaaccgcc ttatcctcgt gaagcatata
     2281 tagtgacgat tgaaaaagga aagccaggac agacggtaac ctggtaccaa ctcagagccg
     2341 atcatcctaa accagactcg ttgatcagtg aacatccgac ggctcaggaa gcgatggatg
     2401 cgaaaaaacg ctatgaggac cctgacaaag agtgaccgca tcagactgct cggaagggat
     2461 tctgagtgcc actacaaggg atctgcgtca catttttcat aattcatgtt tttctaataa
     2521 ttagaatatt aaacaataac aatccattac tggaatcatt tggaatcttt acattatgcc
     2581 gtgcacgtct gctgctacgc tttttgtcat ttgtagcaca agtaagtgtc agcagtggtg
     2641 ctccacactt gcccggtaat taacgacgaa agaaaagtaa ggtggatgaa caatgagtgc
     2701 gtcgttggcg atcctgacca tcggcattgt acctatgcag gaggttttgc cgctcctgac
     2761 ggaatacatt gacgaagata atatttccca tcatagcctg ctggggaagt taagtcgtga
     2821 agaagtgatg gcggagtacg cgccagaagc aggcgaagac accattctca cattattaaa
     2881 tgacaaccag ctggcccatg tttcgcgtcg taaagtggag cgagacctgc aaggtgtggt
     2941 tgaagtgctc gataatcagg gttatgacgt cattttatta atgagtacag caaacattag
     3001 tagtatgact gcgcgtaata cgatctttct tgagccgtcg cgaatattgc ctccactggt
     3061 ttcctctatt gttgaagatc atcaggtggg ggttatcgtt ccggttgagg agatgctgac
     3121 cgttcaggcg caaaaatggc aaattttgca gaaatcgccg gtattttcat tgggtaaccc
     3181 cattcatgat tcagaacaaa aaatcattga tgccgggaaa gaattactgg caaaaggggc
     3241 tgatgtcatc atgctggatt gtttaggatt tcaccaacgt catcgcgatt tactgcaaaa
     3301 acagctcgat gttcctgtct tgctgtctaa cgtattgatt gcacggctgg ctgcggaatt
     3361 actggtgtaa ttttgcgtga cagccagcgc ctctggcccc tatagtgaag tagatgttca
     3421 actaccagac agggccagtt tatgcttcaa agtaatgagt acttttccgg caaagtgaaa
     3481 tcaatcggct tttccagcag cagcactggt cgcgccagcg tgggtgttat ggttgaaggc
     3541 gaata
//
LOCUS       DAJTVJ010000256         3496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000256 DAJTVJ010000000
VERSION     DAJTVJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.260"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            129..1376
                     /locus_tag="OO448_004346"
     CDS             129..1376
                     /locus_tag="OO448_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCR6339361.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="OO448_004347"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="OO448_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCR6339362.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="OO448_004348"
     CDS             complement(2228..2572)
                     /locus_tag="OO448_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339363.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="OO448_004349"
     CDS             2814..3416
                     /locus_tag="OO448_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339364.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          873 a          703 c          807 g         1113 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct agggttgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggagagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaata
//
LOCUS       DAJTVJ010000257         3483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000257 DAJTVJ010000000
VERSION     DAJTVJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.261"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..427
                     /locus_tag="OO448_004350"
     CDS             <1..427
                     /locus_tag="OO448_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6339365.1"
                     /translation="PGKRALNKDEPVFTPIKGVEPPEWFAEEDLPLATIMWQLTTKEL
                     CGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQGNTITGAMGGRVKNPELTAKKEQE
                     SEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLKIIES"
     gene            424..2157
                     /locus_tag="OO448_004351"
     CDS             424..2157
                     /locus_tag="OO448_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6339366.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            2169..2351
                     /locus_tag="OO448_004352"
     CDS             2169..2351
                     /locus_tag="OO448_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339367.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            2351..3483
                     /locus_tag="OO448_004353"
                     /pseudo
     CDS             2351..3483
                     /locus_tag="OO448_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          810 a          868 c         1040 g          765 t
ORIGIN      
        1 ccccggcaag cgagccctga acaaagatga acctgttttt acgcccatca aaggtgttga
       61 gccaccagag tggttcgctg aagaagatct ccctctcgcc acgatcatgt ggcaactgac
      121 aaccaaagaa ctctgcggtc agggcctgct gtgcgtgact gacctggcgg tacttgagcg
      181 gtggtgcgtg gcctatgagt tctggcgacg tgccgtgaaa aatattgcca tacagggcaa
      241 caccatcacc ggtgcaatgg gcggcagggt caaaaatccg gagctgaccg ccaaaaaaga
      301 acaggagtcc gagatgagca gcacgggggc aatgctcgga ctcgacccca gcagccgcca
      361 gcgtctgatt ggcctggcgg ggcagaagaa agccactaac ccgtttctga aaattatcga
      421 atcatgagcc ggaaatctta ccccaacgta aatgctgcaa atcagtatgc ccgggatgtc
      481 gtgcgcggaa agattgttgc ctgccagttt gtgattcagg cctgccagcg ccatcttgat
      541 gacctgatgg cggaaaaaag taagtcgttt cgttaccgct tcgacaagga cctggctgaa
      601 cgggccgcga aatttattca gctgttgccg cacaccaagg gggagtgggc attcaaacgg
      661 atgcccatca cgctggagcc gtggcagcta tttgtgatct gctgtgcgtt tggctgggtc
      721 aataaaggca cccggttgcg ccgcttccgg gaggtgtaca ccgaaatccc ccgtaagaac
      781 ggcaaatcag caatctctgc cggtgttgcc ctgtattgtt ttgcctgtga taacgagttt
      841 ggcgcggaag tgtattccgg tgccacgaca gagaaacagg cgtgggaagt ctttcgcccg
      901 gcgcgactga tgtgtaaacg cacacccatg ctgacggaag cgttcgggat tgaggttaac
      961 gcctcaaaca tgaaccgtcc ggaggatggc gcgcggtttg aaccgctgat cggcaaccca
     1021 ggtgatggtt catcacccca ctgtgccgtg gttgatgaat atcacgagca tgccaccgat
     1081 gcgctttata ccacaatgct taccgggatg ggggcgcgac gtcagccact gatgtgggcc
     1141 atcaccaccg ccgggtacaa cattgagggg ccgtgctacg acaagcggcg ggaagtcatc
     1201 gagatgctca acggctcggt gcctaacgat gaactgttcg ggatcatcta taccgttgat
     1261 gaaggtgacg actggaccgg cccgcaggtg ctggaaaaag ccaatccaaa tattggcgtg
     1321 tcggtttatc gcgaattttt gttaagtcag cagcagcgtg cgaaaaataa cgcccgtctg
     1381 gcaaacgtct ttaaaacaaa acacctcaat atctgggtgt cggcgcgttc ggcgtatttc
     1441 aacttggtga gctggcagag ctgcgaggat aaatcactga cccttgagca gttcgagggg
     1501 cagccgtgca ttctggcctt tgacctggcg cgtaagctgg atatgaacag catggcgcga
     1561 ctttataccc gcgagattga cggtaaaacg cattactaca gtgtggcccc gcgtttctgg
     1621 gtaccgtatg acacggtgta cagcgtcgag aaaaatgaag atcgacggac agccgaacgc
     1681 tttcagaaat gggtggaaat gggcgttctg accgttaccg atggtgcgga ggtggattat
     1741 cgctacatcc tcgaggaggc caaagcggcg aacaaaatca gcccggtcag tgagtcaccc
     1801 atcgacccct tcggggcgac cgggttgtca catgaccttg ctgatgaaga cctgaatccc
     1861 gtcactatcg tccagaactt caccaatatg tccgatccga tgaaagagct ggaagcagcg
     1921 attgaatcgg gacgttttca tcatgacggc aatcccatca tgacctggtg tatcggcaac
     1981 gtggtcggca aaaacatgcc gggtaacgat gatgtggtga aacccgtcaa agagcaggcg
     2041 gaaaacaaaa tcgatggtgc agttgcgctg attatggcgg ttggcagagc catgctgtac
     2101 gagaaagaag acacgctgtc tgaccacatt gagtcctacg ggatccgctc gctttaactg
     2161 aggtaattat gatcatgctg attctcgcgc ctctggtggg cgtgctgggt gcgcttttgc
     2221 tggcgtatgg tgcctggctg atttatcccc cggcgggttt tgttgttgcc ggggcgctgt
     2281 gcctgttctg gtcgtggctg gtggcgcgat atctcgaccg tacacggcag tctgtcggcg
     2341 gaggtaaata gtgttctttt cgggattatt tcaacgaaaa agcgatgtgc cggtgaccac
     2401 gccagcagaa ctgctgatgc tatcgggctg tcatacgaca cctataccgg aaagcggatc
     2461 agtagccagc gggccatgcg cctgacggcg gtctattctt gcgtcagggt gctggcagag
     2521 tctgttggta tgctgccctg cagtctctac aaaatcagcg gtaccctgaa aacacgggcg
     2581 gtggatgaac gactgcataa gttggtttcg gcaaaaccta atggctacat gacaccgcag
     2641 gaattctggg agctggttat tgtctgcctg tgtctacggg ggaattttta cgcctacaag
     2701 gtaaaggcac tgggagaagt ggtggagctt cttccgatag atccgggctg tgtggaaccg
     2761 aagctgaaca gccagtggca gccggtttat caggtgacgt ttccggatgg ttccgtggat
     2821 gtgttgaccc agaatgaaat ctggcatgtg cgcaccctga cgctggatgg acttgtcggg
     2881 ctgaatccca ttgcgtatgc gcgtgaggcc atttcactgg cagcggcaac cgaggagcac
     2941 ggtgccaggt tgtttagtaa tggtgcggtg acatccggtg tgttgcgtac ggaacaaaag
     3001 ctcacgccgg atgcttacga gcgtctgaag aaagattttg aggagtgtca cacggggctt
     3061 ggcaatgctc accgcccgat gattctggaa atggggctgg actggaagtc gatggcgctg
     3121 aacgccgagg acagtcagtt cctggaaacc cgcaagtttc agctggaaga aatctgtcgt
     3181 ctgttccggg tgccgttgca catggtgcag aacaccgatc gcgccacctt caacaatatc
     3241 gaagagctgg ggctgggatt tatcaactat tcactggtgc cgtatctgac ccgcatcgaa
     3301 cagcggatca acaccggact ggtacgaaaa agtaagcagg gcgtttatta cgccaaattt
     3361 aacgccgggg cgttactgcg cggggatatg aagtcccgtt ttgaagccta cgccaccggg
     3421 atcaactggg gaatttactc ttccaatgac tgccgcgacc tggaagatat gaatccgcgt
     3481 ccc
//
LOCUS       DAJTVJ010000258         3443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000258 DAJTVJ010000000
VERSION     DAJTVJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.262"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            143..400
                     /locus_tag="OO448_004354"
     CDS             143..400
                     /locus_tag="OO448_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR6339368.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(450..1400)
                     /gene="uspE"
                     /locus_tag="OO448_004355"
     CDS             complement(450..1400)
                     /gene="uspE"
                     /locus_tag="OO448_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6339369.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1552..2304)
                     /gene="fnr"
                     /locus_tag="OO448_004356"
     CDS             complement(1552..2304)
                     /gene="fnr"
                     /locus_tag="OO448_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6339370.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2499..3014)
                     /gene="ogt"
                     /locus_tag="OO448_004357"
     CDS             complement(2499..3014)
                     /gene="ogt"
                     /locus_tag="OO448_004357"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6339371.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3025..>3443)
                     /locus_tag="OO448_004358"
     CDS             complement(3025..>3443)
                     /locus_tag="OO448_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6339372.1"
                     /translation="VGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSILAP
                     IFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGTYYS
                     LVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT          909 a          822 c          837 g          875 t
ORIGIN      
        1 gaagtagcaa cattagctaa ctaatcaatg cgttgattgt aaatccagct aagaggtgag
       61 gttttcagag cagacaacgg tgaaatgtca tggtattgtt acgtttaggt aacaagaaat
      121 ttgtctgcac aaggattaca tcatgattat ggcgaaactg aagtcagcga aagggaaaaa
      181 atttctcttt ggtttgttgg cggttttcat tattgcggcg tcggttgtga ctcgcgcgac
      241 tatcggcggc gttatagaac agtacaatat tccgctgtct gagtggacga catcaatgta
      301 tgtgattcag tcatcgatga tttttgttta tagcctggtc tttactgtgt tgctggcaat
      361 cccgttggga atttatttcc ttggcggcga agagcagtaa gtaaaaaata ggcccgataa
      421 ctcgggcctt gtcagttatt gaagagtcgt taatcgtctt cttcgtcatc cagttcaacg
      481 ggtgtctgat actggtcagg tttaatgacc aacagatcgc agcgaagatg atcaatcacc
      541 tgttccgccg tgttgccgag gaatgctgct gaaataccgg tgcgtcctac cgtgcccaga
      601 accacaatcc ccgcctgtaa gtgctccgcc aaatcaggaa taacctcttc cggcagacct
      661 ttttctacgt gcgtcatgtt ttcattaatg ccgaatttct gccgcagagc tttcattgcc
      721 agcaaatgtt gcccacgaat ggcatcgtta taaacgctcg gatcaaattc cggcagttca
      781 atcgcgatat taattggcgt tactgggtaa gcgccaacca gatgaacttc ggtatggttg
      841 acttgttctg ccagttcgat cgtctctttg accagttttt cattgagcgc attatgatac
      901 ggctcttcac tggcgagatt caccgccacc agcgccttac ctccttccgg ccacggctgg
      961 tctttcacca tccacaccgg gcttgggcat ttgcgtaaca gatgccagtc cgttggcgta
     1021 aaaatcaccg cttccagacg gtcatgttgg tgcgccattt ttagcaccaa atcgtgcccg
     1081 ccgctgatca cttcctgaat gatggcttcg aagggacggt tatgccagac cactttaatt
     1141 tcaatgggaa cgccagcatt gagataatat tttgcctgct cgtggatcca ggctgtacgc
     1201 tggctgatga cgccctgacg catagcggta cgttcgtccg gggagagcag ggtggtcatt
     1261 tcgtatgaga agtcatagat cggcaaaaag gctttaattt tgccaccaat ccgttgatgt
     1321 aaataaacag ctcgccgcaa tgctggttgg tcgtcctggt taggatcgat aacaacgagc
     1381 atgttctgat acatagccat acagggtctc cttacaacaa ctgtcaacgc agtttgtaat
     1441 taaaagatta acccatatct ggtgaatgaa acagtgatga accttctgcc agatcaataa
     1501 atcagaaaaa tttaatgata tgacagaagg atagtgagtt atgcggaaaa atcaggcaac
     1561 attacgcgta tgaccagcaa gctgggccag cgcatcgtta ttttcgatgg tgatgtattt
     1621 acctttgact gccagcatac cgcttttctg gaagcgacct agcagacggc tgatggtttc
     1681 taccgtcagg cccagatagt taccgatatc gccacgagtc atcgtcaggc ggaattcacg
     1741 aggggagaag ccgcgttggg caaaacgacg ggacaggttg tagatgaatg cagccagacg
     1801 ttcctcggca tttttcttcg acaacagcaa gatcatgtcc tgatcgcctt tgatttcacc
     1861 gctcatcaga cgcatcatct gctgacgcag attcggcatt ttaccggaca aatcgtccag
     1921 cgtttcgaac gggatttcac ataccatcga ggtttccagc gcctgagcga agctcgggtg
     1981 atggccgctg ccgatggcgt caaatcccac caggtcgcca gctaaatgga aaccagtgat
     2041 ttgctcgtcg ccttgctcag tgatggtata acttttaatc gtaccggagc ggatggcata
     2101 aagcgattta agttcatcac cagccttaaa cagcgtctgg cctttctgaa taggcttctt
     2161 ccgctcaatg atattatcaa gctgatcaag ctcatgttcg ttgagtgtga acgggatgca
     2221 aagctggctg atgctgcaat cctggcaatg gatagcacaa ccgccagact gaatgcgccg
     2281 tataattcgc ttttccggga tcataggtct gctcaagccg taattgatat ttgtcaattt
     2341 taacatcttt ttagggagca agtaagtcta agcaaacctt aacagcagag aattccgata
     2401 ttagatgtaa atatatgtct atctatttga aaacccttaa gttgttaagg gtaactttac
     2461 ataaaagtgt gaacaagctg gcacaaattg tttaatgttt acagcaaaag ataaccttcg
     2521 tggcgcaata accactcttt tcgctgaact ccgcctgcat atccggtcat ggtgccgttt
     2581 cggccaataa cccgatggca aggtacgacg atgctgatgg gattcgatcc gtttgccgca
     2641 ccaacggcac gcgccgcgcc agggcggccc aattgttcag ccagttggcc gtaatgcatt
     2701 acctgtccgc aggggatagt gcgtagagtt ttccagactt cgcgctgaaa tggcgtcccc
     2761 cctgtggcag tgggaagcgt atcaataatg ctaagattac cggcaaaata atcacgaagc
     2821 ttgtcgctta aaccgcctgg gttggtggca gaaatgcgct catagccttc tttgcgataa
     2881 tggatgtcca gcagctgcac catgcgttcg ctgtactctt cccattcaac cgcccgcagg
     2941 cgaaattgct catcgcaaat cacccacagt ggacccagtg gcgtggcaat tttttcttca
     3001 agtaatctca gcatccgttc tctcttaaga caaacgtggg taaatccccg gacctatcgg
     3061 cagaccaaca agataccacg ccagcaacat cagcagccat accaccaaaa agataagcgg
     3121 atagggcaag actaacgaat agtaagtacc cagtttcgcg tctggtttgt agcgttgcag
     3181 gaatccaaga aacagtggaa caaaaggaga taccggcgct aaaggcaata cggatgagtc
     3241 ggcaatacga aagaggattt gcgcaaatgc cgggtgaaag ccaagtagca taaacattgg
     3301 tacgaaaatg ggggccagaa tcgaccagat tgcggaaccg ctggcaataa acatgcataa
     3361 gaaagaggaa agcaacgcca gaccgacaaa cgccgggatg ccgctaagcc ctgaactttc
     3421 cagtatatcg gtcagcccca cgg
//
LOCUS       DAJTVJ010000259         3392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000259 DAJTVJ010000000
VERSION     DAJTVJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.263"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(263..463)
                     /locus_tag="OO448_004359"
     CDS             complement(263..463)
                     /locus_tag="OO448_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339373.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="OO448_004360"
     CDS             complement(463..735)
                     /locus_tag="OO448_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6339374.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="OO448_004361"
     CDS             complement(738..1016)
                     /locus_tag="OO448_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339375.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="OO448_004362"
     CDS             complement(1017..1574)
                     /locus_tag="OO448_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339376.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPGKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(3138..>3392)
                     /locus_tag="OO448_004363"
     CDS             complement(3138..>3392)
                     /locus_tag="OO448_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6339377.1"
                     /translation="FDLSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHM
                     GRPVKASSEAVQTLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          943 a          694 c          628 g         1127 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcctg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttgcaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccggaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa
     2221 ttagccatat atagagaact aaaagatttt ttagctattt tgtttaaaag catatcaaac
     2281 gatacatttt attgatttag gtcaaagaca aaagcattga caaaatggta agattgttca
     2341 catgacctta cttaacagac ttttaagaga ctttgaaaca gcctgtttaa caggtcacta
     2401 cctctggtct tatcgttccc ttctttctag gtggttatct gttaagagtt tttcagtttt
     2461 tattcttaag ataattgtta agatgatact ctcccttcgg gttatatcgt atctcttctc
     2521 ccgttaaata tcccttcaca gtcagactaa ataacacctg tgtagttctt ggtagaatct
     2581 ttcaaggtaa cttagctctg agtttatgaa gttgacctat gagatagact ctaccgaatc
     2641 tgttagtcaa ttgttgctta ggtgctgcta aggcttactt agtggaaaga gaggggatat
     2701 gtgaccatag gggaacccct cctgttaaaa gactacttag aagtctgagg ttttagtaaa
     2761 atctgtgaat atggcttctc agacgctctg taacgcattc taagcgattt taccccttta
     2821 ttttaaggtg gttagtacat ccctataggt tacccctaaa aatggggtta aaactcgcta
     2881 cagagctttc tatgagatta gcttctaatc gaatcaacta tgaggtttgg taggattgtg
     2941 caaaatctgt acagtaactt ttcagaatct gaaaattttt ataaaaaatg actagccctc
     3001 tttgaatctc atcttccccg aatcttcaaa aatatctgac agatgtctgt agctgctaag
     3061 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
     3121 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctaac
     3181 tcttcctgaa tctgcaacct cgtcttacct tgtgaaataa gcgtctgtac agcctctgaa
     3241 gaagctttta caggcctccc catatgtacc ccttcagctt tagcacgttc aataccagcc
     3301 tttctacgct ctgctatcaa gcttctttca agctgtgcca acgttgacat aatgcctaac
     3361 attgcttcac ccatagcgct tgataagtca aa
//
LOCUS       DAJTVJ010000260         3380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000260 DAJTVJ010000000
VERSION     DAJTVJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.264"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1396)
                     /locus_tag="OO448_004364"
     CDS             complement(<1..1396)
                     /locus_tag="OO448_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6339378.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAVLFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIR"
     gene            complement(1393..1692)
                     /locus_tag="OO448_004365"
     CDS             complement(1393..1692)
                     /locus_tag="OO448_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6339379.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(1699..2019)
                     /locus_tag="OO448_004366"
     CDS             complement(1699..2019)
                     /locus_tag="OO448_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339380.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(2012..2209)
                     /locus_tag="OO448_004367"
     CDS             complement(2012..2209)
                     /locus_tag="OO448_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6339381.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            2263..2715
                     /locus_tag="OO448_004368"
     CDS             2263..2715
                     /locus_tag="OO448_004368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339382.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            2773..2949
                     /locus_tag="OO448_004369"
     CDS             2773..2949
                     /locus_tag="OO448_004369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339383.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(3061..3270)
                     /locus_tag="OO448_004370"
     CDS             complement(3061..3270)
                     /locus_tag="OO448_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6339384.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
BASE COUNT          850 a          905 c          714 g          911 t
ORIGIN      
        1 ggcgaatcac aaccgccagc tctccttcta ttttttctgc cagcatgggg tcgcgttcgt
       61 tctccggtac aacttccgaa aaattgaata tcacccgacg acgtgagatc cccccgctgc
      121 ggtcactgaa tgacatggcg ttattgttaa ccgccagcac tactgccgga atacgcgttg
      181 agtagggggc tttgtgtttc gggtcaattg ccaccttgtc accgcctgta atggccttaa
      241 tccctgcccc atcaccagcg tagcgggtca tatccggcat gataatcagc gaaaagccaa
      301 ccactaacgc gcgttccctt gcatcttcca gcgccttcat gcttgccgat actgtgttgg
      361 ccttacccgc cagcatggtg caaatctccg ccatcacgct tttaccactt ccccctggac
      421 ctgttacctc aatgaataac tgccagtcgt accggttcgc cagcaccatg aataatacag
      481 ccagtacgcg atccgccttg cggtcattct cagccaccga acggcgtaac cacttccaga
      541 aattcggcgc atgtgttgcc agcgtttccc cctctgctgg tgggctgaaa ggtaattcac
      601 tggcaattaa caaccagtcg tttttgttat gctcccgaaa attacctgtt ctggtatcaa
      661 ataccccgtt actgaatcca atcaggttac gggctgtatt ccccattaca ggcaaactta
      721 acttcatggt atcgaccgcc gatttaatgg cgttctgcga atagctgatc tccgcatcaa
      781 tgaaaatctg tgccatagct cgctgtaatt ctttatcctg tactggctcc catacaacgc
      841 cgttgtaatg gtgaacagtg tcagagtcag catgaatcgc cagttcaccg ccataatgtg
      901 ccaggagaac ttcgccgcgt tgacttgctc ccatctggtt aagcgccagt gatgaagcgt
      961 tatcgtcttt tacccgctct tttttcttta caggcagttc aactaccttt ttcttttccg
     1021 cctgctctgc ccgttcacgt tccagatatt cgcgccagtt ctcccgtttc tggctgtgca
     1081 ttccttcagg gtaataatca gcatccctga cacctgccgc tgccagtttc tgcccgatgg
     1141 tattaacaag ccccggacgc aataaccccg cctggtagag acgcacccga tagcgtccgt
     1201 ccggtacgat ttgcagctta tccagttcgg caagttgttg ttctccaagc cagacaggag
     1261 gcacgctgtc gccagccagt cgcccgtcct gttcctgcca ctgcttcgca tgtgcccacg
     1321 catcattacc cgcaaaaatg atgacttccg tcattttgtc acgcggctgg tgttttaaat
     1381 ttggcgcttt tttcatttct gctctctcca cgcggcaatc atgtttttca gttcctgtag
     1441 ttttttatca acatccatac atgacacatg gttatttctg gaaagcggga tttcccgcct
     1501 gaatctgcta ataaagatct ccacgttcag cgaactatga aatgaatagc catcacgaat
     1561 aaaatacaca cggtcaaaca tcagttcttt taccgttact ctgttaccgt tcttatccag
     1621 ataaatagcg ccggggataa ttttggggtg tgcataaccg ctggcagtca agccagataa
     1681 atacgttctc atgattattt atctcagatt tgaatcagta ttcgctttct ctatggcatt
     1741 taatgcatct gtggcatttt caatggtgca ccgtaacgaa atatcaaact gcccaagcat
     1801 tgccagtaac aagccaatat tacccatgtc aatgcgcata gccttttcgt catattcctc
     1861 attttctgac gcatgccaca tcaggctacc aattgacgca acagccattg atatattgtc
     1921 agtagcccca tccgcagcgg aataaacctt tttagcaata tcatgctcac agttaaaatg
     1981 cggattaatc aggtactggt aattggtcat gtcaggcatg gcacacctcc tgacgaatac
     2041 gggcggcgaa tacagcaaca caacctgacg ggcaacggtt acgcgcttcg cgttccgtcc
     2101 aggcggttac gtggatgatt tgagattctc cggcactcag ggccagaaaa cgccacacaa
     2161 aggccgtttg tgtgtgtaca aggtgtggta tatgatttac ggcaaccata acggctcctc
     2221 gtttacgttg ttggttagaa gccctgcgag tggtaacgac acttgcgggg cttcgtcgtt
     2281 tcaattgctg aattgcatgt atcagcactt gtggtattca cattacattt aggtgaatac
     2341 catttcaagt cttttttggt attcactttt gtattacact gcatcccgtt attaatggga
     2401 ggtgcaaaca tgtccaggag ttctgttaac aataagtcac agcaactgaa tgccagattt
     2461 ccacatgaag tagtgagtgg cattgaggca tccctacagc caggggaaac taaagcgaat
     2521 tttatagtta cggctgtacg cggtgagatc gccaggcgcc aggcagaagg aagtggagaa
     2581 aatccccttg tgtcttcact ggatgcccta gctaatgtcg aacaaatcgg catcaaggca
     2641 gcggaggaaa tcgggcagct tatcaccgtt gcacgcgaag aactccagcg ccgcaaggcc
     2701 aaagaatcag aataatcact atcagcgccg tggtgtgagg aactccggcg cattgcttta
     2761 caggcgcaca caatgaccaa cgaagaatca accaaaaaaa catcacctgc acgaaaaaga
     2821 cgacgcagaa agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag
     2881 aagtacctta aagaatactc atcaaaggag agagcttcgc agttatggca acgcactgaa
     2941 acagactgat agcattgccc accagcctga tagcggctat cattgacatg cttatgttta
     3001 gtgtttccaa actggcgacc gccccaggtc gcctttgttt tatgtgtcat atgctcccct
     3061 ttacactgcc ttacctgaat taatgcgatc ccggctttta acccattcca taacctcgga
     3121 cagcagccac cctacagaac gaccgcccag attaagacgt gacggaaagc gccctttttt
     3181 ctccagttcg taacgtgtag tgcggcacac tccagttaac ttacgacatt catcctcacg
     3241 gattacgcga tcttcattta tttcacgcat acaaaaacct acataaaaat tacgtatata
     3301 aacttttctc ttagctgtag atatatgaga tcggatatta cttagattct ttttcacctc
     3361 ttaaattaaa aacacaacca
//
LOCUS       DAJTVJ010000261         3341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000261 DAJTVJ010000000
VERSION     DAJTVJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.265"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            227..943
                     /gene="nanC"
                     /locus_tag="OO448_004371"
     CDS             227..943
                     /gene="nanC"
                     /locus_tag="OO448_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6339385.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            963..2069
                     /gene="nanM"
                     /locus_tag="OO448_004372"
     CDS             963..2069
                     /gene="nanM"
                     /locus_tag="OO448_004372"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6339386.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2134..3114
                     /locus_tag="OO448_004373"
     CDS             2134..3114
                     /locus_tag="OO448_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6339387.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          997 a          700 c          775 g          869 t
ORIGIN      
        1 attaaacatc agaagagatt ttatactcag gtatttaatc tgattctctg tttatttaaa
       61 taatgtggaa agagaatttt cacaggagac cttatacaaa aatataaaat aagctacccg
      121 ttgccaaaga cactataagc ctggcaaaaa atattacaca acataaatgc taattgttta
      181 tgcgggcttt gtattgcttt ctgtatccta caaatgagtg aaatttatga aaaaggctaa
      241 aatactttct ggcgtattat tactgtgctt ttcgtcacca ttaatttctc aggctgcgac
      301 actggacgta cgtggtggat atcgtagcgg aagccatgcc tatgagactc gactcaaagt
      361 cagtgaggga tggcaaaatg gatggtgggc aagcatggaa agtaatacct ggaataccat
      421 tcatgataat aaaaaggaaa atgccgcact caatgatgtt caggttgaag ttaattacgc
      481 gattaaactt gatgatcaat ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa
      541 cggcacacgc tacggaccct acgtaaaact gtcctgggac gcgacaaaag atcttaattt
      601 tggcattcgc tatcgttacg actggaaagc ttaccgacaa caagacttat ccggtgatat
      661 gtctcgtgat aacgttcatc gttgggatgg atatgtcact taccatatta atagtgattt
      721 caccttcgca tggcaaacga cgctatacag caaacagaat gattatcgct atgcaaacca
      781 caagaaatgg gcgacggaaa atgcctttgt tctacaatac catatgacgc ccgatattac
      841 gccatacata gaatatgact accttgaccg ccagggtgtt tacaacggca gagataattt
      901 atcggaaaac agttatcgca ttggtgtgtc atttaaactg tagtagacag gagacagtca
      961 caatgaataa aactataatg gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt
     1021 ctgtattacc ggaaactcct gtgccattta aaagtggtac cggagcaatt gataacgaca
     1081 ctgtctacat tggtttaggt agcgcaggta cagcatggta caagctagat acacaggcca
     1141 aagataaaaa atggactgcg ttagctgcat tccctggtgg accgagagat caagcaacct
     1201 ctgcatttat tgatggcaat ctatatgtgt ttggcggcat tggcaaaaac agcgagggat
     1261 tgactcaggt atttaatgac gtacacaaat acaaccccaa aaccaatagc tgggttaaat
     1321 tgatgtcgct cgcgccgatg ggcatggcgg gtcatgtgac ttttgtacac aacggcaagg
     1381 cttatgttac tggcggtgtt aaccagaata tcttcaatgg ctattttgaa gatctcaacg
     1441 aggctggaaa agattcaacc gctatagata aaatcaacgc ccactatttt gacaaaaaag
     1501 cagaagatta tttcttcaat aagtttctgt tgtcttttga tccctcaaca cagcaatgga
     1561 gttacgctgg cgaatcgccc tggtacggaa cggctggtgc ggcggttgtg aataaaggtg
     1621 ataaaacctg gcttattaat ggcgaagcca aaccaggatt gcgaacggat gccgtatttg
     1681 aacttgattt caccggtaat aatttaaaat ggaataagct tgatcccgtc tcatcaccag
     1741 atggcgtcgc tggcggtttt gcggggataa gcaatgattc tcttatattt gccggagggg
     1801 ccggattcaa aggttcacga gaaaattacc agaacggtaa gaactatgcg catgaaggcc
     1861 tgaaaaaatc atatagcact gatattcatc tttggcataa cgggaaatgg gataaatcgg
     1921 gtgaattatc gcaaggtcgg gcctacggag tatcattgcc ctggaataat agtttattaa
     1981 ttattggcgg tgaaactgca ggcggcaaag cggtgacgga ttcagtttta atttctgtga
     2041 aggataataa agtcacagta caaaactaac gcttcagggc cccggtaaag gggccctgct
     2101 atcaatttgt catttgtatt acgcgaaaat tatatgaacg caataatatc gcccgattat
     2161 tactatgttc ttaccgttgc tggtcagtct aatgccatgg cgtatggcga aggactgcca
     2221 ttaccggaca gggaagatgc gcctcattcc agaattaaac aattagcgag atttgcgcat
     2281 acgcatcccg gaggcccgcc atgtcacttt aacgacatta ttccactgac tcactgccca
     2341 cacgatgttc aggatatgca gggttatcac catcctctgg caacgaatca tcaaacacag
     2401 tacggcaccg ttggccaggc actgcatatt gcacggaaat tactgccctt cattcctaat
     2461 aatgcaggga ttcttatcgt tccgtgttgc cgtggcggat cggcttttac cgcgggcagc
     2521 gaagggacat attcagaacg gcacggagcc agccatgatg cttgtcgttg gggaacggat
     2581 actccgctgt accaggattt agtcagcaga acgcgagccg cactggcaaa aaatccgcag
     2641 aacaaattcc tcggcgtatg ctggatgcaa ggcgaatttg acttaatgac cagtgactac
     2701 gcgtcacacc ctcaacactt taatcatatg gttgaagcct ttcgtaggga tctaaaacaa
     2761 taccattctc agcttaataa tattactgac gcgccatggt tttgcggcga taccacctgg
     2821 tactggaaag aaaatttccc gcattcgtat gaagctattt atggcaatta tcaaaataat
     2881 gttttagcca atattatttt cgtcgacttc cagcaacaag gtgaaagagg actgacgaac
     2941 gcgcctgatg aagacccgga cgatttaagc acgggatatt acggttcagc gtaccggtca
     3001 ccggagaact ggacgacggc actgcgaagc agtcatttca gcacggcagc ccgtcggggg
     3061 attatttctg acagatttgt agaagcaatt ttgcagtttt ggcgcgaaag gtgagcgggg
     3121 agtttattca tgggggatgt ccttgttcgt tctcagagta gacaacaaaa aacaaataag
     3181 atcaaggtaa tatctcttca cttgcgggct cgaacagcag gcaattgcca atataataat
     3241 ctggcacaag ggtgaaaaac gcgcaccgca taaaccgttg ttattgctta cagattagcc
     3301 ggatgcctta atagacatcc gcgccttttc gattatggct c
//
LOCUS       DAJTVJ010000262         3323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000262 DAJTVJ010000000
VERSION     DAJTVJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.266"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OO448_004374"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OO448_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6339388.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..1086)
                     /gene="fliA"
                     /locus_tag="OO448_004375"
     CDS             complement(367..1086)
                     /gene="fliA"
                     /locus_tag="OO448_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6339389.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1251..2825)
                     /locus_tag="OO448_004376"
     CDS             complement(1251..2825)
                     /locus_tag="OO448_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6339390.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3080..3323
                     /locus_tag="OO448_004377"
                     /pseudo
     CDS             3080..3323
                     /locus_tag="OO448_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          852 a          791 c          712 g          968 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
      541 cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc gctgatgatc
      601 atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag
      661 ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg
      721 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
      781 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
      841 ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg
      901 tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc
      961 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
     1021 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
     1081 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
     1141 ttttttaatc ggacgattag tgggtgaaat gaggggttat ttaggggtta ccggtaaatt
     1201 gcggacagaa aaaaccccgc cggtggcggg gtttgagcga taagtgtaaa ttaacgcagc
     1261 agagacagta cgttctgcgt ggtctggtta gcctgtgcca gaacagaggt acccgcttgt
     1321 tgcaggatct gcgcacgaga catgttagac acttcggttg cgtagtcagc atcttctata
     1381 cggctacggg cagaagacag gtcgtttacg gtgttgccaa ggttggtgat ggcagagtcg
     1441 aaacggtttt gtactgcacc caaagaactg cgcaacttat caacttttgc caaagcttcg
     1501 tctaatttgg ataatggatt tgtggttgct aaggctttca aatctgaggc actcagttca
     1561 gcacctttgg ttgccgtctt attcgtgata ttggctgtat aaaccttatc atcttcacgc
     1621 ataattgcct gtgcagaacc atcatttttt actgcccatt caactgcctt accattagga
     1681 tcagtaataa cagacgtgtt acctgtagcg gcatctacat ttactgcaat gtaggtgccg
     1741 tctgatgttt tagcagcaaa tgaattaggt acaggattcc cttgggtatc ttgtacttgg
     1801 tggacatcta ggttttttcc cccaaaataa acagacattg gtgcatcatt ataagctaat
     1861 gctttaccca ttttacccgc agctaaatca actgatgtgg cagaaccatt atcttcaaca
     1921 aatgcagtta cacgaccacc aaagtttgct aactgaacat ccttaccacc cacctgaact
     1981 ttttcataac ctgtagagtt ttgtttcaat aatgtaaaac cgttcgtgtt ggtattatta
     2041 tcttgaccaa caaaataagt ctgcccttcc acctgtactt gataaacttt tgttcctgca
     2101 gtaccggtcg catcagttgt atcctttgta tcttgtacat taccggtacc aaaataagtt
     2161 tcaacatcag catttttata attgctaccg aatgctttag cacctcccac actaccatca
     2221 gttgcattta ccttagttaa gtcaggagcg gcttcgctct taagtgcaac cgcagaggac
     2281 attggaactt ttcctagtgc tacgctaaag ttatcaagcc ccaaggtttt agcatcaatt
     2341 ttatcaaggg caatctcaat ggtttcacca tcgttcgcac ctacttgaat tttcatagtc
     2401 tgatcagaag ccagaacttt tacaccatta aattgtgtct ggttagaaac acgatcaatt
     2461 tcactaaggc gttgtgtaat ttcatcctga attgaattca ggtcggagtc agagtttgta
     2521 ccattagtgg cctgtactga caattcacga atacgctgta agttattgtt gatttcagaa
     2581 agcgcacctt cagtggtctg cgcaacagaa ataccatcat tcgcgttacg ggaagcctga
     2641 gtcagacctt taatatttgc tgtaaaacgg ttagcaatcg cctgacctgc tgcgtcatct
     2701 ttagcactgt taatacgcag gccagaagag agacgttcaa tggcggagct cagagaagac
     2761 tgagatttgt tcaggttatt ctgggtcaac agcgacaggc tgtttgtatt aatgacttgt
     2821 gccatgatct tttccttatc aattacaact tgatgttatt gggctgttgc ccacggtttc
     2881 tcaccgtacc ccttgtatcg gcacctgaat ttcgaacttt agaaaatttt agtttctctc
     2941 ctgttttttc ttaacggctg aaatatccgt aatatacaac tctactctgc gcatcatctt
     3001 cgtgaatcaa ctattaaact ttgcgcacaa attgccgata acccgcttat ctactgtttt
     3061 caatcaaaag gaattaggta tggcttcatt tacatctctt ggcgttggtt cgaatctgcc
     3121 gctggataca ttgctaaaca acctgaccat cgctgaaaaa aaacgcttaa acccaattac
     3181 tcagcaacag agtgacaata ccgcacgtct gacagcctat ggcacattaa aaatgtgcat
     3241 tagaaaaatt ccagactgca aatactgcgt taaataaagc cgatctgttc aaaagcacta
     3301 ccgttaccag tagcaatgaa gac
//
LOCUS       DAJTVJ010000263         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000263 DAJTVJ010000000
VERSION     DAJTVJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.267"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="OO448_004378"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="OO448_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="OO448_004379"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="OO448_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCR6339391.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..>3258
                     /gene="sfmF"
                     /locus_tag="OO448_004380"
     CDS             2834..>3258
                     /gene="sfmF"
                     /locus_tag="OO448_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HCR6339392.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTL"
BASE COUNT          802 a          740 c          842 g          874 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc tcgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacact
//
LOCUS       DAJTVJ010000264         3257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000264 DAJTVJ010000000
VERSION     DAJTVJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.268"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            201..353
                     /locus_tag="OO448_004381"
                     /pseudo
     CDS             201..353
                     /locus_tag="OO448_004381"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            330..509
                     /locus_tag="OO448_004382"
                     /pseudo
     CDS             330..509
                     /locus_tag="OO448_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            648..1379
                     /locus_tag="OO448_004383"
                     /pseudo
     CDS             648..1379
                     /locus_tag="OO448_004383"
                     /inference="COORDINATES: protein motif:HMM:NF019126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1780..1920
                     /locus_tag="OO448_004384"
                     /pseudo
     CDS             1780..1920
                     /locus_tag="OO448_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1931..2512
                     /locus_tag="OO448_004385"
     CDS             1931..2512
                     /locus_tag="OO448_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6339393.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT          967 a          718 c          823 g          749 t
ORIGIN      
        1 cggcggcgtc atcggcatca tcggcgtctg cttcaaaaga tgaggcgacc agacaggcgt
       61 cagcagcgaa gggcagcgcc acgacggcat ccacgaaggc gacagaggct gctggtagtg
      121 cgacggcagc agctcagagc aaaagtacgg cggaatctgc agcaacgcgc gctgagacag
      181 cggcaaaacg ggcagaggat attgcatccg ccgtggcgct tgaggatgcg agcacgacga
      241 aaaaggggat agtacagctc agtagtgcga ctaacagcac ttccgagtca ctggcggcaa
      301 caccaaaagc cgttaaggcc gcgtatgacc tggctaacgg gaaatacacc gcacaggatg
      361 caacgacagc acagaaaggg ataatccagc taagcagcgc gaccaacagc acgtctgaaa
      421 cgctggcggc aacgccaaag gcagtaaaag cagccaatga caatgctgag aaacgtctgc
      481 agaaagatca gaacggtgcg gatatccctg gcaaagacac ctttacgaaa aatattggtg
      541 cctgccgtgc cttcggtggg tcagtaagca caacaacagg aaactggacg actgcacagt
      601 ttatcgagtg gctggattct cagggagcat ttaaccatcc atactggatg tgcaagggtt
      661 cctggtctta tggcaataat aaaatcatta ctgatactgg ctgcggtaat attcatctcg
      721 ccggagctgt cattgaagta atggggataa agtcagcgat gacgatccgc attaccactc
      781 cgaccacatc cagcggtggc ggaacaacca acgcccagtt tacctatatt aatcacggaa
      841 cagattattc acctggctgg cgaagggact ataactccag aaataagcca acggcatcag
      901 agatcggggc gctaccgtca ggtggaacag cagtatcatc agttaatctg gcttcaaaag
      961 gtcgggtagc cgcgctgaca gacaatacgc agggggcagc aggtcttgag ttatacgagg
     1021 tgtataacaa cggatatcca acagcgtatg gaaatatcat tcacctgaaa gggatgacag
     1081 ccgttggcga aggtgagtta ctcatcggct ggagtggtac aagcggtgct catgctccgg
     1141 catttattcg ttcacgacgg gatacgaccg acgcaaactg gtcgccgtgg gcgcagcttt
     1201 acacctcggc tcatcctcct gcagagtttt atccagtcgg tgcaccaatc ccgtggccat
     1261 cagataccgt tccgtctggt tatgccctga tgcaggggca gacttttgac aaatctgctt
     1321 acccgaaact tgcagccgct tatccgtcag gcgtgatccc tgatatgcgt ggctggacga
     1381 ttaagggcaa gcccgccagt ggtcgtgccg tattgtctca ggaacaggat ggcattaaat
     1441 cgcacaccca cagcgccagc gcatccagta cggatttggg gacgaaaacc acatcgtcgt
     1501 ttgattacgg cactaaatcc acgaataaca ccggggcgca tacgcacagt ctgagcggct
     1561 ctacaagttc agctggggcc caccaacata gtcagactgg gcccagaacg aatagcggga
     1621 gtcaacctac tggaatgttt ccggcaggga gtactcaagt tagcgggact aatcaggttg
     1681 gtatcagtgg ctcattaacc agtggcacct cccaatgggt aggtaaatca tcttcagaag
     1741 gaaaccatac acactcactg tccggcacag cagcatctgc gggtgcacac gcgcatactg
     1801 tcggtattgg tgctcatacg cactctgttg cgattggttc acatggacac accatcaccg
     1861 ttaacgctgc tggtaacgcg gaaaacaccg tcaaaaacat cgcatttaac tatattgtga
     1921 ggctcgcata atggcattca gaatgagtga acaagcacgg accataaaaa tttataacct
     1981 gctggccgga actaatgaat ttattggtga aggtgacgca tatattccgc ctcatacagg
     2041 tctgcctgca aacagtaccg atattgcacc gccagatatt ccggctggtt tcgtggctgt
     2101 tttcaacagt gatgagtcat cgtggcatct cgttgaagat catcggggta aaacggttta
     2161 tgacgtggct tccggcgacg cgttatttat ttctgaactc ggctcattac cggaaaatgt
     2221 cacctggtta tccccggaag gggagtttca gaagtggaac ggcacagcct gggtgaagga
     2281 tacggaagca gaaaaactgt tccggatccg ggaggcggaa gaaacaaaaa acagcctgat
     2341 gcaggtagcc agtgagcata tagcgccgct tcaggatgct gtggatctgg agatcgcaac
     2401 ggagaaagaa accttgttgc tggaagcttg gaaaaagtat cgggtgttgc tgaaccgtgt
     2461 tgatacatca actgcaccgg atattgaatg gccagtagca cctatagggt aaattcgtaa
     2521 tgattaccta aatacgttat tcttttgtta aaaaagtgat tttgctatag gtagggagta
     2581 tgttcttgga ttaagaataa aaaatcgcca tgaaagagct tcatggcgag tacataaacg
     2641 tacaaaatgt gatgaaagta gagtggcgta agccactata ttaagatatg acaatagcga
     2701 agaaaagtaa ataacatttt atggacgttt aattgttaat tataaaaact agagtgcccg
     2761 atattgttta tcttgcttta aaataatgta ctcaaaccaa atttttgagc taaaagctca
     2821 aataatataa atgcaataat aatgtttaat aagacaatta gcttgctttg tattttcatt
     2881 tcggaatact gttaagtaac tttgatgacc ctaacaatag atcggaattt tcttagagtc
     2941 aatggtcact gtttttgctt gcaaagcaaa tgttttggtg gttataagtc gtcttaatat
     3001 agtcacgcat gcaacttgtt ccatgcggag attaaagatt ggaatataac gcgaagatgg
     3061 aattaaatta catttgcaat aattaattta ttattactca ataattaccg atattattat
     3121 tgtaaattaa tcattagaat gcaaaaagaa atgtaacggc aaaataagag gacgatagct
     3181 gagaaaaaag cgtgctttcc caatctgggg caaatcacgt ctgaatgaca cctgaaaaca
     3241 ggtggtagcc tcaagaa
//
LOCUS       DAJTVJ010000265         3222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000265 DAJTVJ010000000
VERSION     DAJTVJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.269"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            8..511
                     /locus_tag="OO448_004386"
     CDS             8..511
                     /locus_tag="OO448_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6339394.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(557..973)
                     /gene="rraB"
                     /locus_tag="OO448_004387"
     CDS             complement(557..973)
                     /gene="rraB"
                     /locus_tag="OO448_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6339395.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1135..2139
                     /gene="argF"
                     /locus_tag="OO448_004388"
     CDS             1135..2139
                     /gene="argF"
                     /locus_tag="OO448_004388"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6339396.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2211..>3222)
                     /locus_tag="OO448_004389"
     CDS             complement(2211..>3222)
                     /locus_tag="OO448_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6339397.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPG
                     YVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGW
                     NN"
BASE COUNT          893 a          721 c          675 g          933 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
      481 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg
      541 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
      601 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
      661 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
      721 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
      781 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
      841 tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc ggatcgctgc
      901 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
      961 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     1021 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     1081 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg acctatgtcc
     1141 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
     1201 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     1261 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     1321 ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc aagcggcagc
     1381 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     1441 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     1501 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct ggcggatctc
     1561 ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct agtctatgca
     1621 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     1681 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
     1741 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga
     1801 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagag
     1861 aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa gatgatgcag
     1921 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     1981 acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     2041 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     2101 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     2161 cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt tcaattattc
     2221 caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt tcctaactgt
     2281 tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt catcattctt
     2341 gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac ataacccgga
     2401 agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc taactcatta
     2461 agatacagct gttcgccctc ataacactta agtaattgat agaagtacca cttagcaaaa
     2521 tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat gctgacaggc
     2581 acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg tgatttttct
     2641 tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat caattcctgt
     2701 aatttacggc tatttttata gggttcttta tcgagattga aaaataaggt ctcagctatt
     2761 ttaattttag attgatcgca gctaaaatct ttagattgat cgcagctaaa atcaatatga
     2821 tttatcacgg agcatgcata ctttatcttt tcagaatcac caaaatattg cattctttta
     2881 ttaaaacacc aatccagtat tcttacatca ttaatatatg gggattttaa aaaagtctcc
     2941 agaatcgaat cgatgaccgg tgtaaggtta acttttgcat catctaagga atcaataaga
     3001 atatgagaca ggttaacttt gatgtcatta atttcattgt cagtagcttt cttattatta
     3061 agaagataat tatttactgc gttaaggtca tctatttctt tcagtatccc tttttcacca
     3121 agcaagtatg aaatcgattc attacataca taaggcatta atagcagatg ttctttcctt
     3181 attgctaata atgcattgtt caatggtttt tctaataaat cc
//
LOCUS       DAJTVJ010000266         3200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000266 DAJTVJ010000000
VERSION     DAJTVJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.270"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(55..582)
                     /gene="mobB"
                     /locus_tag="OO448_004390"
     CDS             complement(55..582)
                     /gene="mobB"
                     /locus_tag="OO448_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6339398.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(564..1148)
                     /gene="mobA"
                     /locus_tag="OO448_004391"
     CDS             complement(564..1148)
                     /gene="mobA"
                     /locus_tag="OO448_004391"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6339399.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1218..1487
                     /locus_tag="OO448_004392"
     CDS             1218..1487
                     /locus_tag="OO448_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6339400.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1564..2550
                     /gene="srkA"
                     /locus_tag="OO448_004393"
     CDS             1564..2550
                     /gene="srkA"
                     /locus_tag="OO448_004393"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6339401.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2567..3193
                     /gene="dsbA"
                     /locus_tag="OO448_004394"
     CDS             2567..3193
                     /gene="dsbA"
                     /locus_tag="OO448_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6339402.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          820 a          772 c          776 g          832 t
ORIGIN      
        1 gcagaaaaaa atgccggatg cgacactggc gcgtcttatc cagcctactc ttgcttatcc
       61 gttctgcttt tgcatccact ccaccacaaa atcagccagc ccctcaacat cattaatatc
      121 cagtaatgcg acatcaagat taagcggcac atcactggct acagcaataa catgcctgtc
      181 tatcactaat tcttcaggtc gatgtccggc tccatcgcga aacagcacaa tctttgcgat
      241 ctcttcatgc ttaaaccctt cgaccagaat caaatccagc tttgaggtat ccattcgact
      301 tgcgagaaac tgtagatcca gctcttcttc gtctggtttt tccgtcatca aggcccatcg
      361 ttgctggctg gcaacgatgg tttgtgccgc gccagccttg cgcagctcat agctatcttt
      421 gcctggctta tcaacatcca tatcatggtg cgtatgctta atcagccctg gacggatccc
      481 tctggcgcat aatgccggga tcagtttttt caaaagcgta gtttttccgg taccactcca
      541 cgcggcaaag gcgagtaacg gtatcatcgt ttttcctgcc atcgggcaag ctcctctggc
      601 gtattcacgt taacaaatgc atctttatga tcgctgaaat caaccgcatg gccgccagcc
      661 agacgcataa atgccattac ccggcgttct cctgcttgca gatattctag taataaaggc
      721 tcaatagcac ggtttaccag agcaatagtc gggtgatcgc gttcaccgtc atggacccac
      781 acgacaggcg catctttgcg ctgatgatta agccgggaag ctaaaacatg gggaatgtaa
      841 ggcgtatcgc acggacaaaa caaaaaccac tcaccagctt cctgctgcat tactgaaagc
      901 attcctgcca gagggcctgg gtaatccgcc agtgaatctt caatcacttt cagaccgctt
      961 gcctgataga tttcctgatg acgattggca ttaaccacga cgtgagagag ctgcgtcata
     1021 agcgcgtcag cgacatgttg ccataatggt ttgccgttta attcaagcaa tcctttatct
     1081 acgccgccca ttcgtctggc tttaccgcct gccagcacaa cgcctgttat cgtcgtcatc
     1141 agattcaccg atatcgcctc ttttattgtg ggattgaccc tgctaacgtg tctgtctcaa
     1201 gagtaaggag catcactatg aaatgtaaac gtctgaatga agttattgaa ctcctccagc
     1261 cagcctggca aaaagagcca gaccttaacc tgttgcaatt tttgcagaaa ctggcgaaag
     1321 agtcaggttt tgacggcgaa ctggcggatt tgacggatga cattctgatc tatcacctga
     1381 aaatgcgtga ttccgcgaaa gatgcggtga tcccgggttt gcagaaagat tatgaagaag
     1441 acttcaaaac ggcgctgtta cgcgctcgcg gcgtaattaa agagtaaaag cttgtaagcg
     1501 gcgccaccaa aatcatcgtg aaatgatatc cttcatcatt cgtaatgttt tccggatgat
     1561 gggatgaaca acagcgcttt tactttccag acactacacc cggataccat catggacgct
     1621 ctgtttgagc aagggatccg ggtggattcc ggtcttaccc cgcttaacag ctatgaaaac
     1681 cgtgtctatc aatttcagga cgaagagcgt cgacgttttg tcgtcaaatt ttatcgccct
     1741 gaacgttgga cagccgatca aatcctcgaa gaacatcaat ttgcgttgca gctggtaaat
     1801 gatgaagttc cggtcgcagc acctgtggcc tttaacggtc agactttatt gaatcatcag
     1861 ggattttatt tcgctgtttt tccaagcgtc ggtggtcgcc agttcgaagc tgataatatc
     1921 gatcagacgg aagcggttgg gcgttattta gggcgtatgc accagacggg gcacaaacag
     1981 ctttttatcc atcgcccgac catcggtctg aatgaatatc tcattgagcc acgcaagctg
     2041 tttgaggacg ctacactgat accttccggg ttgaaagcgg cattcctgaa agcgacagat
     2101 gagctgatcg ccgccgttac agcacactgg cgggaagatt tcaccgttct gcggctacat
     2161 ggagactgcc acgccgggaa tattctctgg cgcgatggtc caatgtttgt tgatctggat
     2221 gatgcacgta atggtccagc tattcaggat ttgtggatgt tgctcaatgg cgataaagcc
     2281 gagcagcgga tgcaactgga aactattatt gaagcttatg aagaatttgg cgagttcgac
     2341 accgctgaaa tcggactgat tgaaccttta cgcgccatgc gtttggttta ttatcttgcc
     2401 tggctaatgc ggcgttgggc tgatcccgcg ttcccgaaaa atttcccgtg gttaaccggg
     2461 gaagattact ggctgcgaca aacggcgact tttatagaac aggcaaaagt tctacaagaa
     2521 ccccctttac aattaacacc tatgtattaa tcggagagag tagatcatga aaaagatttg
     2581 gctggcgctg gctggtttag ttttagcgtt tagcgcatcg gcggcgcagt atgaagatgg
     2641 taaacagtac actaccctgg aaaaaccggt agctggcgcg ccgcaagtgc tggagttttt
     2701 ctctttcttc tgtccgcact gctatcagtt tgaagaagtt ctgcatattt ctgataatgt
     2761 gaagaaaaaa ctgccggaag gcgtgaagat gactaaatac cacgtcaact tcatgggtgg
     2821 tgacctgggc aaagatctga ctcaggcatg ggctgtggcg atggcgctgg gcgtggaaga
     2881 caaagtcact gttccgctgt ttgaaggcgt acagaaaacc cagaccattc gttcagcatc
     2941 tgatatccgc gatgtattta tcaacgcagg tattaaaggt gaagagtacg acgcggcgtg
     3001 gaacagcttc gtggtcaaat ctctggtcgc tcagcaggaa aaagctgcag ctgacgtgca
     3061 attgcgtggc gttccggcga tgtttgttaa cggtaaatat cagctgaatc cgcagggtat
     3121 ggataccagc aatatggatg tttttgttca gcagtatgct gatacagtga aatatctgtc
     3181 cgagaaaaaa taattcaatg
//
LOCUS       DAJTVJ010000267         3192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000267 DAJTVJ010000000
VERSION     DAJTVJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.271"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..549
                     /locus_tag="OO448_004395"
                     /pseudo
     CDS             1..549
                     /locus_tag="OO448_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            556..1302
                     /locus_tag="OO448_004396"
     CDS             556..1302
                     /locus_tag="OO448_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6339403.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1317..1739
                     /locus_tag="OO448_004397"
     CDS             1317..1739
                     /locus_tag="OO448_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6339404.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1797..2153
                     /locus_tag="OO448_004398"
     CDS             1797..2153
                     /locus_tag="OO448_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339405.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2322..2987
                     /locus_tag="OO448_004399"
     CDS             2322..2987
                     /locus_tag="OO448_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6339406.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          882 a          712 c          885 g          713 t
ORIGIN      
        1 ttcaggttgg atggctggtc gaaaatgacg gagggctttc tctacctaac tttgaacgtc
       61 ataacggaaa aagctctaaa aaaaacgggc ggttacaaac gagcgagtaa caaaaatacg
      121 cgaactgaaa cgaaaaggta acgctgccag cgttacacaa acggatcaaa aagcgttacc
      181 agaggaagag gaagaggaag atctaaatac tgatctcccc ctcgccaaaa acgagcgtct
      241 aaaaaattcg agccggaggc tattgagctg cccgattggt tgccggaaac actctggcat
      301 gagtgggtcc ggttcagaca ggcattgcga aaaccgattc gaacggagca gggcgctaac
      361 ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga gcaggtgatt
      421 cgacacagca tcgccaatga ataccagggc ctgttcgcgc cgaaaggtgt tcggcctgag
      481 acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc gccaggcttc
      541 agggggtaac ggaccatgaa aaatattgcg acaggcggcg ttctggaacg catccgccga
      601 ctgaccccgc cacatgtaac cgccccattc agaacggtag cggagtggcg cgagtggcaa
      661 cttgctgaag gccagaaacg tagcgaggag atcaaccgcc tgaatcgcca gttgcgggtg
      721 gaaaaaattc tgaatcgctc aggcatccag ccgttgcacc gtaaatgctc gtttgcgaat
      781 taccaggtgc agaacgacgg ccagcgatac gcgttaagcc aggcgaaatc catcgccgat
      841 gaactgatga ccgggtgtac aaattttgcg ttcagcggaa aacctggtac cgggaagaat
      901 cacttagcgg cagctatcgg gaatcgcctg ctgaaagacg gtcagacagt gattgtggtt
      961 accgtggctg atgttatgag cgccctgcac gccagctatg acgacgggca gtcaggcgaa
     1021 aaatttttgc gggagctgtg cgaagtggat ctgctggttc ttgatgaaat tggcattcag
     1081 cgcgagacga aaaacgagca ggtggtgctg caccagattg ttgatcgccg gacagcgtcg
     1141 atgcgcagcg tggggatgct gacaaacctg aactatgagg tgatgaaaac attgctcggc
     1201 gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt taactgggag
     1261 agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga ggatttatgg
     1321 cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg gaactcgtgc
     1381 atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc agtcgtgcgt
     1441 ctgtcggtcg cttatgcata gaactggtcg caagtggtga tgtatataat tctggctacg
     1501 gcttattccc gtctgaacag gctcgcaagg actggcaaag cgcccgcaaa aaactctcga
     1561 gagtaaaggt gaggaaaccg gttgttgttg atccggacct tatctggtca ttacctgacg
     1621 gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc cggaagagcg
     1681 aagctatgca gcgtgtactg gctttctatc agggtaattt tcaggaggcg atactgtgaa
     1741 tgaaattagc tatcaggctt caattaccgc tggcattcgc atcaaaggag aggagcatgg
     1801 aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa gaggatgacc
     1861 ctcgtaatca ggttaatttt ctttatgagc catcggaaag accatattgc catcacgcct
     1921 ctgtacgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt gcggttgtgg
     1981 agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca gatgatgtaa
     2041 ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg atacagattg
     2101 agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa taattaaatt
     2161 tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca aatcatcagg
     2221 agaccacccg aaagggcggg gagcagtcac acatctgttt ccgatagccc cgttctaatg
     2281 ctacactctt tgatattttt atgaccccaa taaacatatt tatgacagtt gctgatttca
     2341 aacggcccaa attggagctc ccaaacgggg caaacaaact actactgcac tcttgctgtg
     2401 ctccatgttc cggtgaggtg atggaggcgc ttcaggcctc gggaatcgac tacaccatct
     2461 ttttctacaa cccgaacatt catcctcaga aagagtattt aattcgtaag gatgagaata
     2521 ttcgctttgc tgaacaacac ggcgtgccgt ttattgatgc tgattacgac acagacaact
     2581 ggtttgaacg agccaaagga atggaatggg agcccgaacg agggatccgt tgcaccatgt
     2641 gttttgacat gcgttttgag cggacagcgc tgtacgccgc tgaaaatggt ttcagtgtga
     2701 tcagcagttc actgggcatt tcacgctgga aaaatatgca gcaggttaac gactgtgggc
     2761 ggcgagccgt cgcgcattat ccgggcatgg tgtactggga ttataactgg cgcaagcagg
     2821 gcggctcgtc ccgtatgatt gaaatcagca agcgcgaaaa attctatcag caggaatatt
     2881 gtggctgtgt gtattctctg cgcgatacca atctacaccg caaatctcag ggacgccctc
     2941 ttatcaaaat tggtcaactc cactacggta aagaagagaa ggagtgattt tatggggcac
     3001 ctttctgatt gatttcatat tggcgaggta agtagaatga ctgcgggtgc ttgaggctat
     3061 ctgcttcagg catgaacacc aaaaggcaga tagagaaaag ccccagttaa cattacgcgt
     3121 ccggcaagac gcttaacatt aatctgaggc caatttcatg ctttgcacat gtaggttagc
     3181 ctcttacatg cc
//
LOCUS       DAJTVJ010000268         3165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000268 DAJTVJ010000000
VERSION     DAJTVJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.272"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            533..2386
                     /locus_tag="OO448_004400"
     CDS             533..2386
                     /locus_tag="OO448_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6339407.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2537..2752
                     /locus_tag="OO448_004401"
     CDS             2537..2752
                     /locus_tag="OO448_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6339408.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2757..3071
                     /locus_tag="OO448_004402"
     CDS             2757..3071
                     /locus_tag="OO448_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR6339409.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          777 a          759 c          881 g          748 t
ORIGIN      
        1 cttccaggcc catccgctcc ggtgggcctt tttactgcag aaaacaggtt ccccgttaaa
       61 tgctatgttg ctcacaattc agtaagttga cagttgcctg tcagactggg catttgttaa
      121 aaaaatttcg catggtgaat ccccctgagc ggaggggcga ctggtgacgg tataatctct
      181 gattatcaaa acgagaatga cgcgggttta gtggcaccgg gctgaactca ccgggaggca
      241 cccggcacca tgtgcatgat gatacagata cgcggcttta gcccctctcc ggaggggttt
      301 tcttgtgggc aaaaaaagcc cgcgctggga gacgcgggcg gcaaggaata aacaataaaa
      361 cgtgaagtaa tatttcagct ggcgaataat accccatagt aatcactctg cgcaactgcg
      421 cggtcttttt cgaattgcgg gctgtcgtct ctcttctgcc attgtcctgt aacttccgga
      481 cttcagcccg ctccttattt tactcacaat attatcccgg ccgggaggat tcatggcatt
      541 taaacactat gacgtggtca gggcggcatc gccgtcagac cttgcgaaac gactgacaca
      601 aaaactgaag gagggctggc agccgtttgg tagtccggtg gccataaccc cttataccct
      661 gatgcaggcg attgcagcag aaggtgatgt ggtcgtcagt ggtgcaactg agccggagtg
      721 gtactacgtc atcgtactgg ccgggcaatc caatgccatg gcttacggtg aaggtcttcc
      781 gcttccggat tcatacgatg cgccccatcc gcgcattaag caactggccc gtcgtaacac
      841 agtgactccc ggtggtgaag tatgcgtatt taacgacatc attcctgctg accattgtct
      901 gcatgatgtt caggatatga gtacgattaa ccatccccgg gctgacctga gcaaagggca
      961 gtacggctgt gtcggacagg gcttacatat tgccaaaaaa ctgcttccgt atatccctaa
     1021 taatgcgggg atcctgctgg taccatgctg tcgtggtggt tcggcattca cccagggcac
     1081 ggaggggaca ttcagcgagt ccacgggggc cagtcaggat tcggctcgct ggggagtggg
     1141 taagccgtta tatcaggatc tgcttttccg cacgaaggca gcattgcaga aaaacccgaa
     1201 aaacgttttg ctggcgatat gctggatgca gggggaattc gatatgacga atgccagtta
     1261 cgcccagcag ccagcagcat ttcttgcaat ggtacagcag ttccgtgctg accttgccgg
     1321 gctggcggcg cagtgtcacg gtggaagtcc ggcatcagtc ccctggattt gtggcgacac
     1381 gacatacgcg tggaaacaag aacacggtac gcaatatgaa gtggtatatg gtgcatataa
     1441 aggtaaagaa tcccagcaga tttattttgt tccctttatg accgatggta gcggagttaa
     1501 tacaccgaca aacaacccgt cagaagatcc tgatattgcc gggtctggtt attacggttc
     1561 ggcatcccga acgaacaaaa actgggtatc atcaaatcgc ccgacgcatt tcagctcatg
     1621 ggcgcgtcgt ggcattattc ccgatcgtat ggcaactgct attctgaacg tagccggtcg
     1681 caccttagcc ttcattagtg gtaaggcacc ggaaatcaaa ccctcgcccg gcggcgacac
     1741 gccatcgggg ccgtctgaag atgcatccat acgcacaatc tccctgttgc cgacagccgg
     1801 agacgctgct gcgcagggct ggagcattaa gaatggcgga gttcagttgt cagatggtgt
     1861 atttaagatc accaagcaga gcaataaagc ctggtccctg acgcgcccgg tggatgacgc
     1921 agtctccctg ctgacacggg gtggcagact gagctgtaag tttcgactgt caggcgcact
     1981 gaccaacaac cagttcggtc tgggaattta tctgtatacc gatgtagcgt tacctgacgt
     2041 cgtggcgatg accgggactg gtaacccgtt cctgatgtcg ttcttcaccc agaccacaga
     2101 cggcaaactg aatctgatgc atcacaagaa agcaggaaac acaaagttgg gcgagttcgg
     2161 gaattacagt aacgactggc tgacgctgga gctggtgttc accgccggca gtgccacggt
     2221 tactccgaaa ctgaatggag tggctggccc ggcattccag gtcataaaag acagtctgac
     2281 actggggctg aatgcgctga cgctgacgga tattaccaaa aatgcagcgt atggcgttga
     2341 gatagaaagt ctggtgctgg agataaatgc accggcatca tcataaaaag tgagccagcc
     2401 aaatggaagg tatcgttaaa ctcaccggta gtgtcagtgg gtcgtctgag atgcctgcat
     2461 gagttatcag agccatcagt acttaactgg tggctttttt tattgttgtc agcttccgga
     2521 taacgggaga cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac
     2581 gtcagcggtg ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca
     2641 gtgggcggca ataggcgtgc tggggagtct gctgtttggg ctgctgacat atctgactaa
     2701 cctgtatttc aaaatcagag aggaccgtcg taaggctgca cggggagagt aattcaatga
     2761 ctcaaaacta tgaactgatt gtgaaaggga tccgcaattt tgagaataaa gttacggtaa
     2821 ctttagcgtt acgggacaaa aaacgctttg acggtgaaat ttttgacctg gacatctcgc
     2881 tggaccgtgt tgaaggtgcc gcgctggagt tttatgaggc agcagccaga aggagcatca
     2941 gacaggtctt cctggatgtt gctgccgggt tatgtgaagg ggacgagctg ttgccagaaa
     3001 cgcgcccctg ttcagaggcg cggtatacca taaaaattaa cagttctgat aactcgatta
     3061 cgggttgtta gctttttgca gttggctttc cagtatcttt cattggtagc atcctgataa
     3121 atatccatga gcgcaaaaat caaatacggc ctgtcagctg ctgtt
//
LOCUS       DAJTVJ010000269         3105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000269 DAJTVJ010000000
VERSION     DAJTVJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.273"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(110..3022)
                     /locus_tag="OO448_004403"
     CDS             complement(110..3022)
                     /locus_tag="OO448_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339410.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPKGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
BASE COUNT          784 a          834 c          744 g          743 t
ORIGIN      
        1 atctgctgga gcaggatttt tacgccagtg gtccgaacca gaagtgggcg ggagacatca
       61 cgtacttacg tacagatgaa gttcggttac atcctgtcag cacagaacct cacgcttttt
      121 gagcggcacg acggatcccc cccacccagt atccgacaga cttttcactg cgttcgtaaa
      181 aacccttctt ccctcccagc atatccacta attccggtga catatcctgc agataagtct
      241 gcaaatcatg ccccagatct tccggtgact gcaactcata aaccgcgcgc cacacatcac
      301 cctgccttat tttaagcccc gggttaagaa tatactgtcc gtggttcagc ctcagaaaaa
      361 gcttttttcc ataaggattt ttgctgttaa cttcatgttg agagagctgg ctgctgatat
      421 aactccttcg tttgcggcgt tccggcagca acgcttcagg aagatgaacc acacactctg
      481 ccagtcgtgc cgcactgaag gcttccgaaa aaaacagagc acttcccaga tgcgtgtgga
      541 acagcgcctg catcaggatc accagaaaca ttgccatctg ccggttgtcc agtttgatga
      601 ggcgtccttc cacctgcagt gacagacttt caggtttcag cagggtatac aactgcgcac
      661 ttttctgttg cgcataacgg ggcgtactga ccgccgctga cagaacttga tgataagccg
      721 ccagcccgtc attaccggtc agcaagggat ctgcaccacg ctccagcagt aactgaacca
      781 gtgcaatatt acctgcccgg gctgctgaca tcagcggagt aagattaaac ggcgaacggt
      841 gatcaacacc ataacgttca atatcccgca acacggcaac cgggttattc attgagtatg
      901 gccggaaata cttcagctcc agtaactgca gagtcttcgt tctgggctgc cttgtgggct
      961 taaaatcttc ccggtacagt gcattataca gcgggaacag ggaataaatc agactgtact
     1021 ccagcgcctg cagttgtatg gttttatcgg ctgtacctgc gaggatatgc tgtttccact
     1081 gtcgtgcatc ctctgcggtg ataatctgcc atggcatctc agatgtctgc aggatccggc
     1141 tacggatggt gtccgcctgc tcctgcttcc cctgtttttc aagcagatgt gcggttttct
     1201 gccactcatc acggctggaa atctcttcct gcagattcag aggctcttcc tgatgcgtga
     1261 gcgcaagcag actccacaga ggatgctcca cctgctgttc aacaagatac acattgtgtg
     1321 tagcacgggt gagcgcaaca tacagcgcgt tggtaaaaaa tttatagatt tccgctgaac
     1381 gatcctgcct gtcccgcgga cgggaataac gggcctcgcc ctcaagatca gccggtgtaa
     1441 gaccctcgca aatatcatca aatgcctgcc gggcagcgct tactatattg taaaggatca
     1501 ccgtttcgta ttccagcccc ttaacctcct gaaccgaaaa gaccagcggc gtactgaacc
     1561 agcatcgcgc ctgtgctttc tgttccggat gcatcacaat gaccgctaca cgattcgaaa
     1621 gtgaggtttt ggcattcagt tcctgctttg tctcttccct gtcgtccagc aaccggatgg
     1681 tcccttctgc ctgaccgcag cttctgacaa aatggtgact ttcccgatca atagcactaa
     1741 agcggacctg tttgagtctc agcaaccggt tagccagtgc agtgacgtgg tgcccgttac
     1801 ggtaattcgc ctgcaggata ttaacggttg tgtcgtttcc ctgttgctgc cggaaaaaga
     1861 gacttttgag cgaactccat gagaaaaagc tcggatgaac tatctgattg gcatcaccac
     1921 agagtaaaaa ctgaccggga tgacgtagcg ttttcagtac cagttgcagt tgaacaggcg
     1981 tcatatcctg aacttcatca acaaaaatga catcataacg gggagttgcc aacgaaagat
     2041 actgatgact gagcagatta ctgtcatact gatgcgactg tttcagccat gcgatataac
     2101 gttcaaacag gacatagacc gtcgggcggt cctccatgcc atagagcgac tgccgtatcc
     2161 ccagtgacag atacgcctcc cgggacagcg gcccattccc ggaggcaacc gcaccgatca
     2221 cgcccctgaa ctcctcatac agggtatggg cagcccccag tgcggcgata gccctgttgc
     2281 ggggcagcca gtcgctgaac gcactgaaag tcacctccct gcctttcgga atgcgcagag
     2341 tttccagaaa ttccgtcagc gaaagaaagt caatattctg tacctcactc ccttcccctg
     2401 aagcgtcata gagagtacgc gctttttcca ccagaaatga ggaaaggctg agatacagta
     2461 tatcaccagc agcctgcttc attttttcca gtaccagcgc cgtcttacca cttcccgccg
     2521 agccaacaat caccagcggt agcgggtgtt cgtacagtgc ctgctgatca tcatcaaagg
     2581 acagcatttt atccaggcgg tggaaacgcc catgagacgg attgatatac gtcagcgctt
     2641 cagtcgcgat atcaacagga tccggcactg gttgttgttg taagcgccct tcatcaatca
     2701 ccacgtttct gcgaagaaaa cgtgaagtgt tataggcatg attgcgaata tattccagaa
     2761 caaggcagat agtttcaccg tggtagcgat acagcgaaaa cagtaatcgg tcccggatac
     2821 tgagccgggc gcggtaaaga ttggtgtcaa ttttacgaac atcagcctga acaaagttat
     2881 cggtggcaat agcctgtgca aattttttat aacccgggat cgtgtcagga ttcagagtat
     2941 gataaagaca gattttcatg gctgccggtt tccgtcgtgc aaaaaacatg ctgattttac
     3001 gaaggaatgc cttactgaac aaccttgccc ggaatacccc ccgaaatcag atggaacaat
     3061 cagaaaaatg ctctgcagtt atggaataaa gaaggtgggg tggta
//
LOCUS       DAJTVJ010000270         3091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000270 DAJTVJ010000000
VERSION     DAJTVJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.274"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..309)
                     /locus_tag="OO448_004404"
     CDS             complement(<1..309)
                     /locus_tag="OO448_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCR6339411.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     R"
     gene            complement(306..692)
                     /gene="trbI"
                     /locus_tag="OO448_004405"
     CDS             complement(306..692)
                     /gene="trbI"
                     /locus_tag="OO448_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCR6339412.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(689..1939)
                     /locus_tag="OO448_004406"
                     /pseudo
     CDS             complement(689..1939)
                     /locus_tag="OO448_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(1941..2123)
                     /locus_tag="OO448_004407"
                     /pseudo
     CDS             complement(1941..2123)
                     /locus_tag="OO448_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2145..2456)
                     /gene="traL"
                     /locus_tag="OO448_004408"
     CDS             complement(2145..2456)
                     /gene="traL"
                     /locus_tag="OO448_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCR6339413.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(2471..2821)
                     /gene="traA"
                     /locus_tag="OO448_004409"
                     /pseudo
     CDS             complement(2471..2821)
                     /gene="traA"
                     /locus_tag="OO448_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(2867..>3091)
                     /locus_tag="OO448_004410"
     CDS             complement(2867..>3091)
                     /locus_tag="OO448_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6339414.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          711 a          887 c          756 g          737 t
ORIGIN      
        1 ccgcccttcg ttatcccgga tatccgcagc cagtctgacg gacgggtcaa acaaccggct
       61 gctgtatttc tccgtgcgtc cgataccggg cacggcaggg ggccgcagac tgttgcggat
      121 cacccgctcc ttaaaggcat ccattttccg gcccatctca ccggactgct ccagtgccgt
      181 caggcgctgc atgatcaccg tcagcatgtc cggctccttt accggccaca gatcacccca
      241 ggtgccaaga tcggcggcgg ccacactctg cccccatatc agcagggcaa tcagcccccg
      301 gcatctcatg gttccgctct cattcgccgg gcaatatcct gctggatttc ccgggtgata
      361 tccggagccc cctgtaccac ggcaggcgac accagaatga ccgcgtggtg tttctgctgc
      421 cacgcctgca ggctggcttc cagtgccgtg ttaaaacgcg ctgaaagggc ttttgactgt
      481 gcttctgaca gttgtttctg actggcgctg tcaaaaaatg catccacagt ctgtttcatg
      541 ttgaaggcgg cagtcatcgg tgcgttcagt ctgacaatgc cgtaactgac ggctgcgttc
      601 agtaacacca ttgccaggca tcccggcacc gtccaccacc agtggctgcg tcggggggct
      661 gtaacatcag tcgttttttg cgttgtggtc atgcaacact cctgtatttc tcatgttctt
      721 ccagccaggc ttccagcgaa gccatctccg gccctgactt cttccacgcc agctgccaca
      781 ccgcctcatg aatactcagc ccctctttac gtttctgctg cacaaactca aaatccgggc
      841 cgtcagagct gtacatggcg cggcttaacg ggtccacaaa caggcggtgc caggaggaat
      901 ggttttccac ctgcagcagg aaggaactga accactggtc tttggccgca ccaaacttac
      961 caatcatgtc gcgctgcagt ggctggaact ggtccggata cagctggttg tattttgcga
     1021 actccttcgc actctgtttg aggataattt tgtaggagga gttaccccat gccgcacggg
     1081 ccgcactgga ggccttgtca gagtcaaagt cgacgatgtt ctgtgtgatg gtgatataag
     1141 caccggtatg acgacgggcg gtacggtaac ctttctcaat aaactcaccg accttgtgat
     1201 ttttgaagtc cagcagacgc cagccttcat caatgacgtt cagtttcttg aggttacgcg
     1261 gcgtgcggta catcctgttc tcgatgtaga taatcaggga gaacatcacc gcaaccagca
     1321 gtgacggacg gtcttccagt ccgcccagct ccagcaccac catttttgcg tcatcccgca
     1381 gggacggctc atcagagtta aaatactggc cgtaagtgcc gttggcagtg tactggtcaa
     1441 gcagcacaat catttcgtcc agacggctgc ggatagtcgg tgacccggca tactgctcgc
     1501 tgtcactggc gtttttcagg aaatccacca cgtcatcaat acgtgccctg ttctctttgg
     1561 ccagccagga ggccctgacc gcctgcagca gcagaccttc atgcacttca tccaggttac
     1621 cgttggggct ggccatcact gacaactggt cacggacacg ctccgctgac tggtcgatat
     1681 cggtgatgtt cgcaaacggg ttaaaacgca gggtttcacc gtccagatat acgccaccca
     1741 tgttctcaca cagggacttg tagccatccc ccatgtcgaa caccacggca aagcccccgg
     1801 agtccagcac gctgcggata agtggctgta tcagcccggt tttaccggcc ccggaggtgc
     1861 cacagaccgc catgttgtag ttggtgttat tcattccccg gaagaaaata tcgataaacg
     1921 ccagctggtt acggtaggtg tgacacggca aagggcacat taaatcccat tggggtcacg
     1981 gcagtgcgtt gctcattctg aagacggtag ttattcaccc cctgaatgag gttaacagag
     2041 aggctgagaa caataagcac tgacataaat ataaaggcga tggccattac acgactggta
     2101 cttaaacggg caccgtgttc catataacaa tcctggtatc agttctattt aatccactgc
     2161 cggaaacacg aatcgggaac attatgaaaa ataccgcgca gcagggctgt tggcatatac
     2221 cagtaaatca ggtcacgtaa ccaggaactg ccccgccctt ttttcagttt tttaatcccg
     2281 aaataaacca gaaccgctgc accaataccg aacagatatt tcgatgttgt gataccccag
     2341 ccaacacaga ttgctgcggg gatcagttca tccagtggca ggccaaacca gcggctctgg
     2401 ttggtcagtg tttccgggaa acgatatttc ttaagtttat tctcgtctcc caacatcgtt
     2461 ttatttcctg tcagaatccg gcaacagaca tacctactgc aataaataca gagagtatgg
     2521 caaagccgac caggaacttg acgtttttgg tcatcatgta catgactgca ccgaccagaa
     2581 cttcagccag aacaacccat ttaacgatac tggagtcctt accgaaggtc gccttaaccg
     2641 tttcctgacc tttagccatc aggtccgtgc ctttaccagc agccatagct aactcaggga
     2701 acaacatcat cagaacagca accgggatca ctgcacgaac caggcgaagc atattcagac
     2761 gagtgaattt agaaaaaaac gatttctttt tgacgggcgc agaagtaccc tgaacactta
     2821 acgctgaacc gccccgggtt tcctggagag tgttttatct gtgaactcag gctgccagat
     2881 catcgtttcc gatggaagca taataagctt tttctgcttc tgccggagga gtatggccca
     2941 gcctttccag caatcgtcga ttgttatacc agtccaccca cgtgagtgtg gccagctcca
     3001 cttctgtccg gtttttccag ctcttacggt gtattacctc cgctttgtaa aggcccttga
     3061 tgatctctgc catcgcgttg tcatacgagt c
//
LOCUS       DAJTVJ010000271         3073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000271 DAJTVJ010000000
VERSION     DAJTVJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.275"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            194..907
                     /locus_tag="OO448_004411"
     CDS             194..907
                     /locus_tag="OO448_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6339415.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            939..1697
                     /locus_tag="OO448_004412"
     CDS             939..1697
                     /locus_tag="OO448_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339416.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1743..>3073
                     /locus_tag="OO448_004413"
     CDS             1743..>3073
                     /locus_tag="OO448_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6339417.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNA"
BASE COUNT          697 a          739 c          820 g          817 t
ORIGIN      
        1 ggaggtgtta ttgactgcat gaatatatcc tttaatttaa acatccatta aaaatattta
       61 tttggttaat atgtttttat gaaagcgtaa ttcaggtcaa tgtcacaatt aaccattgtc
      121 acaataaggt tgaacggata ttctgagcac ggaccacgta atattcaata cattatttac
      181 tgccgggttt ttcgtgaatc aacgtaatat gtcaataatt aattccacgc cagtgcgtgt
      241 tattgccatt gtaggatgtg atggttcagg taaatcgccc ctcacggcaa gcctggtaaa
      301 tgaactggca gcaagaatgc caacagaaca catttatctc gggcaatcgt ccgggctaat
      361 tggcgaatgg atttcacagc tccctgttat tggcgcacct tttgggcgtt atctgcgaag
      421 taaagcggca catgtgcacg aaaaaccctc aacaccgcct ggcaatatta ctgctctggt
      481 tatctatctg ctttcctgct ggcgggcgta caagtttcgc aaaatgttgt gtaaaagcca
      541 gcaaggcttt ctgctcatca ccgaccgcta cccgcaggtt gaagtgccgg gttttcgctt
      601 tgatggcccg caattggcaa aaaccacggg cggtaacggt tggataaaaa tgttaaggca
      661 gcgcgagctg aagctgtacc aatggatggc atcttatttg cccgtattgt tgattcgtct
      721 tggcattgat gaacaaaccg cgtttgcgcg taaacctgac caccaactgg cagcgttaca
      781 ggaaaaaatc gccgttacgc cgcaactgac tttcaacggc gccaggatcc ttgaactgga
      841 tggtcggcaa ccagccgatg aaatcctgca agcgtcacta cgtgcaattc acgccgcgct
      901 ttcctgatca ccaaaatgga taggggatga tttaacgaat ggatgcacta caaacacaaa
      961 ctgttaatag tacaaccgcg ccgcagccca gctacattcc ggggctaatt gcggtggtcg
     1021 ggtgcgatgg caccggtaaa tccacgctga ccaccgatct ggtgaaatca ttgcaacagc
     1081 actggcaaac cgagcggcgc tatctggggc tgctctcggg cgaagacggc gacaaaatca
     1141 aacgattacc gttggttggc gtctggctgg aacggcgact ggcggccaaa tcctcgaaaa
     1201 cccaaagcat gaaaaccaaa tctccggcgc tatgggcggc ggtgattatg tactgcttat
     1261 cgctgcgaag aatggcgaat ctacgcaagg ttcagcgact ggcgcaaagt ggcgttctgg
     1321 tggtcagcga tcgcttcccg caggctgaaa tttcgggctt ttattacgac ggaccgggga
     1381 ttggcgtcga acgtgcgacc gggaaaatca gcatgtttct ggcgcagcgc gaacggcgtt
     1441 tataccaaca aatggcgcaa tatcgcccgg aattaattat tcgcctgggc attgatattg
     1501 agactgccat ctcccgcaag cctgaccatg actatgccga gctgcaggac aaaatcggtg
     1561 tcatgtcgaa gattggctat aacggcacaa aaattcttga gattgattcc agagcgccct
     1621 acagcgaagt gctggaacag gcacagaagg cggtttctct ggttgccatc gtttctgacc
     1681 gccgaagttt aacttaaggc tggcgggata ctttttacta tcaacgataa ggtcaggctg
     1741 aattggcggg ttttaacatc aaacattggt ttgcagatgg cgcgtttcgc accattattc
     1801 gcaatagcgc ctggttaggc tccagtaatg tcgtgagcgc cttgctgggg ctgttggcgc
     1861 tctcgtgtgc cggtaaaggg atgacgcccg ccatgtttgg cgtactggtg attgtgcaat
     1921 cgtacgccaa gtcgatcagc gattttatta agtttcagac atggcagctg gtggttcagt
     1981 acggaacgcc agccttgacc aacaataatc cgcagcaatt tcgcaatgtc gtctcatttt
     2041 ccttctcgct ggatatcgtc agcggcgcgg tggcgattgt cggcggcatt gccttactgc
     2101 cattcctttc ccattcatta ggtctggatg accaaagctt ttggctggcg gcgctgtatt
     2161 gcacgctcat tccttcaatg gcttcctcca cgccgaccgg cattctgcgt gcggtagatc
     2221 gcttcgattt aattgctgta cagcaggcga cgaaaccttt tctgcgcgca gcggggagcg
     2281 tcgtagcctg gtattttgac tttggttttg cgggttttgt tattgcctgg tacgtgtcga
     2341 atctggttgg cggcaccatg tactggtggt ttgcagcgcg cgaattacgc cgcagaaata
     2401 tccataatgc cttcaaattg aatctgtttg agtctgcccg acacatcaaa ggcgcgtgga
     2461 gttttgtctg gtcaaccaac attgcccact ccatctggtc ggcgcgtaac tcgtgcagca
     2521 cagtgctggt ggggatcgtg ttaggacccg ctgccgccgg gttatttaaa atcgccatga
     2581 cattctttga cgccgccgga acgccagcgg gtttgctggg taaaagtttt tacccagagg
     2641 tgatgcgttt agatccgcgc accaccagac cgtggttgtt aggtgtgaag tccggtttac
     2701 tggcgggcgg aatcggtatt ctggtggcgc tcgcggtgtt gatcgccggc aagccgctca
     2761 tttcgctggt gtttggcgtt aagtatctcg aagcgtatga cctgattcag gtgatgctgg
     2821 gcgcaattgt gatttcgatg ctgggtttcc cgcaggagtc attgctattg atggcgggaa
     2881 aacagcgcgc ttttctcgtg gcgcaaacca tcgcctcaat cggctacatc gtattgctgt
     2941 tcatgttctg tcatctgttt ggtgtgctgg gagcagcgtt tgcttacttc ggtgggcaat
     3001 gtctggatgt ggtgctctcg ttaattccaa ctttaaaggc attttttcag cgtcattcct
     3061 tgctttataa cgc
//
LOCUS       DAJTVJ010000272         3046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000272 DAJTVJ010000000
VERSION     DAJTVJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.276"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..173
                     /locus_tag="OO448_004414"
     CDS             <1..173
                     /locus_tag="OO448_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339418.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="OO448_004415"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="OO448_004415"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6339419.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="OO448_004416"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="OO448_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3046
                     /locus_tag="OO448_004417"
     CDS             2191..>3046
                     /locus_tag="OO448_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCR6339420.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          711 c          836 g          803 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcaccg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggcgaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggat
//
LOCUS       DAJTVJ010000273         2996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000273 DAJTVJ010000000
VERSION     DAJTVJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.277"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..493
                     /locus_tag="OO448_004418"
     CDS             <1..493
                     /locus_tag="OO448_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6339421.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="OO448_004419"
     CDS             493..1083
                     /locus_tag="OO448_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6339422.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..2110
                     /locus_tag="OO448_004420"
     CDS             1070..2110
                     /locus_tag="OO448_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6339423.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYPPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2113..2658
                     /locus_tag="OO448_004421"
                     /pseudo
     CDS             2113..2658
                     /locus_tag="OO448_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2682..>2996
                     /locus_tag="OO448_004422"
     CDS             2682..>2996
                     /locus_tag="OO448_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6339424.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLIAPDEGDLKPDV
                     VKR"
BASE COUNT          776 a          660 c          852 g          708 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattaccccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacgacga ctcaaagccc ggcacgctga
     2941 atgactttct gattgctcct gatgagggcg acctgaaacc ggatgtcgtc aaacgc
//
LOCUS       DAJTVJ010000274         2995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000274 DAJTVJ010000000
VERSION     DAJTVJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.278"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..1077
                     /locus_tag="OO448_004423"
     CDS             19..1077
                     /locus_tag="OO448_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR6339425.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1163..2779
                     /gene="bglH"
                     /locus_tag="OO448_004424"
     CDS             1163..2779
                     /gene="bglH"
                     /locus_tag="OO448_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6339426.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2806..>2995
                     /locus_tag="OO448_004425"
     CDS             2806..>2995
                     /locus_tag="OO448_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HCR6339427.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSL"
BASE COUNT          839 a          632 c          746 g          778 t
ORIGIN      
        1 gatcggctgt ttgatgaaat ggcgcaggcg gggatcaggc cgctggtaac gttatcccat
       61 tacgaaatgc catatgggct ggtgaaaaac tacggcggtt gggctaatcg ggcggtcatc
      121 gatcacttcg agcattacgc ccgcacggtc tttacccgct accaacataa agtagcgtta
      181 tggctgacgt ttaatgaaat caatatgtcg ttacacgcgc cattcacggg cgtggggctg
      241 gcagaagaga gtggcgaggc ggaagtttat caggcgatcc atcatcaact ggttgccagt
      301 gcgcgggcag ttaaagcctg tcatagcctg atccccgaag cgaaaatcgg caatatgctt
      361 ctcggtgggc tggtttaccc cctcacctgc cagccacagg atatgttgca ggccatggaa
      421 gagaaccggc gctggatgtt ctttggtgat gttcaggcgc gtggccagta tcccggctat
      481 atgcagcgtt tcttccgcga ccacaatatc accattgaga tgactgaaag tgacgcagaa
      541 gatttaaaac atactgtaga tttcatctct tttagttatt acatgactgg ttgtgtttcc
      601 cacgacgaaa gcattaataa aaatgcgcag ggcaacatac tgaatatgat ccccaatccg
      661 catctgaaaa gttcagagtg ggggtggcaa attgatccgg ttggattacg ggttctgtta
      721 aatacgcttt gggatcgtta tcaaaaaccg ttatttattg tcgagaacgg attaggcgca
      781 aaagacagcg ttgaagcgga tggttcgata caggacgatt atcgaattgc ctatttaaac
      841 gatcacctgg tacaggtaaa tgaagcgatt gccgatggtg tggatattat ggagtacacc
      901 agttgggggc caattgattt agtcagtgca tctcattcac aaatgtctaa gcgctacggc
      961 tttatttatg tggatcgtga tgataatggc gaaggaagcc tcacaagaac gcgtaagaaa
     1021 agcttcggat ggtatgcaga agtgatcaaa acgcgggggc tgtcattaaa aaaataacca
     1081 ataaagcacc ttaattatcg tcgcattcag aacagtctgg atgcgatacg ttaattcttt
     1141 ctttgcacca taaagggata ttatgtttag acgaaatctt attacctctg ccatcttatt
     1201 aatggcaccg ttagcctttt ctgcacaatc attggctgaa tcattaacgg tggaacaacg
     1261 ccttgagtta ttagaaaaag cgttaagaga aacgcaaagc gaactcaaaa agtataaaga
     1321 tgaagagaag aaaaaatata cgccagcgac ggtgaatcgt agcgtaagta cgaatgatca
     1381 agggtatgcc gccaatccgt tcccgaccag tagtgccgca aaacctgatg ctgtactggt
     1441 caaaaatgaa gagaaaaatg ccagtgagac aggttcgatt tattcttcca tgactctgaa
     1501 agatttcagt aagtttgtga aagatgaaat tggctttagt tacaacggct attaccgttc
     1561 tggttggggg accgcctctc atggttcacc taaatcatgg gcgattggtt ctctgggccg
     1621 ctttggtaac gaatactccg gctggtttga tttgcagtta aaacaacgtg tctacaacga
     1681 aaacggcaaa cgggttgatg ccgttgtgat gatggatggt aacgttggtc agcagtactc
     1741 taccggctgg tttggcgata acgccggtgg cgagaacttt atgcagttct ccgatatgta
     1801 cgttaccacc aaaggtttcc tgccctttgc gccagaggct gatttctggg tgggtaaaca
     1861 cggtgcgccg aaaattgaaa tccagatgct ggactggaaa acgcagcgta ctgatgccgc
     1921 agcgggtgta ggtctggaaa actggaaagt cggtccgggt aaaattgata tcgcgctggt
     1981 tcgcgaagat gtcgatgatt acgatcgcag cctgcaaaac aaacagcaga ttaataccaa
     2041 tactattgat ttacgctata aagatatccc gttatgggat aaagcgacct taatggtgag
     2101 tggtcgttat gtcacggcaa acgaaagcgc atcggaaaaa gataatcagg ataataacgg
     2161 gtattatgac tggaaagata cctggatgtt cggcacatct ttaacgcaga aatttgataa
     2221 aggtggcttc aacgaattct ccttcctggt tgcgaataac tctatcgcca gtaactttgg
     2281 ccgttatgct ggcgcaagtc catttaccac ctttaatggt cgttattatg gtgatcacac
     2341 cggcggaaca gcagttcgtc tgacctcgca gggcgaagcc tatatcggcg atcattttat
     2401 tgtggctaac gcgattgttt actccttcgg taacgatatt tatagctacg aaacaggcgc
     2461 acactctgat ttcgaatcta ttcgtgcggt tgttcgcccg gcctatattt gggaccaata
     2521 taaccagaca ggtgttgaac tgggctattt cacccagcaa aacaaagatg cgaatagtaa
     2581 taaatttaat gagtctggtt ataaaaccac gctcttccat acctttaaag tcaataccag
     2641 tatgttgacc tcgcgtccgg aaattcgttt ctacgccacg tatatcaaag ctctggaaaa
     2701 cgaactggat ggcttcacct ttgaagacaa taaagacgac cagtttgctg tcggtgcgca
     2761 ggctgaaatc tggtggtaat ggaacttaat gaaaggaata ttacgatgaa tataaaaatt
     2821 gctgctttaa cgctggctat tgccagcggt atttctgctc agtgggccat agcagcggat
     2881 atgccagcca gcccggcacc cactattccg gttaaacagt atgtgactca ggtcaatgcc
     2941 gataacagcg tgacctttcg ctactttgcc cctggggcaa aaaacgtctc cttag
//
LOCUS       DAJTVJ010000275         2941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000275 DAJTVJ010000000
VERSION     DAJTVJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.279"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1086)
                     /locus_tag="OO448_004426"
     CDS             complement(<1..1086)
                     /locus_tag="OO448_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6339428.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSA"
     gene            complement(1243..1452)
                     /locus_tag="OO448_004427"
     CDS             complement(1243..1452)
                     /locus_tag="OO448_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339429.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1475..2185)
                     /locus_tag="OO448_004428"
     CDS             complement(1475..2185)
                     /locus_tag="OO448_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6339430.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2701..>2941)
                     /locus_tag="OO448_004429"
     CDS             complement(2701..>2941)
                     /locus_tag="OO448_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339431.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          863 a          614 c          483 g          981 t
ORIGIN      
        1 agcggatact ttaggaaaaa catctttaga aagaacctta ccgtctttca tggccttgtc
       61 taattcttcc gtagtcatcc caagacttct ggcaaataac tgaaaagcac ctgccattct
      121 gtcacctaat tgaccgcgta gctcttcagc ctgaatcttt cctttagatg ccatctgctc
      181 gaaagctttt aaaatcccgt taatttcatc ggtgcttagc cccattaatc tggatgtacc
      241 aatcacatta gaaaataagg attctttctg gtctgcattc atatcaccag caccagcgga
      301 aaacttagca aaactgctta atgtttcaat ctggtctaca ccactactat cagcaagatt
      361 cttaacaaat tctgtagctc gtttagcacc ttcatcacct aaaacgaatt gtgccgctcg
      421 ttgtgatgct tcttttttca gaccaacctc cataatccgc ctgtaagctt ctaaggctgt
      481 ctgaaaacta taaatggctg tacctgcttt gattgcactc gtagctaata catccagact
      541 atttgaagca ttttcactgt gattattttt gctggtggtc ttgttaaact tagaaagtgt
      601 ttcacgaaga gactgattta actttgctaa ctcacgatta aaagtttcgt ttccaagtgt
      661 cttggattta ctgcttaagc tgtaatactg gcttttaaaa tacttctcaa gcataggatt
      721 aaagtcacga agcttgctca tattcgctga gtaagctgct gtgattgctc tgcttccgct
      781 aattcgtggc tgtcttggtt gtctaagtgg ctttaaagta tcctcaattt cagttttaat
      841 cttaaacgct ctagtgtttt taagacgctg ctcaatcttg gaaacagttt tatcgacatt
      901 tctttccatt agtttgagag aatcatttaa aggcgtagga ttaactctaa acgacttatt
      961 caatcgcttc tcaattcgtg atgctacctg catcgtttgt ttctcggctt tttccaagcc
     1021 ttttaaaact ttctgagagt taaaacttaa ctcaatcaga aaatcatcaa ctttaaattg
     1081 tgccattcat atttttcacc tctttaagcg tgttgtacgt aaattctatt tttcagtgtg
     1141 ggtattagct ttacagattt cacgtagcac acatacaaaa aaagggagag ctaccgatgt
     1201 cactcgataa tctctccccg tatttaccca accaattctt ttttaatgtc ggacaagata
     1261 atgtaattcc gtttcaccta tgatttgagc atcagaagga atacggtcaa tacaccactt
     1321 tggaatcttg cgtgtttttg gtttgtgtga ggtgataact tgacgtttgt ttttaattct
     1381 taaggctggc ttagtttgta actttaagtt ttcacaaaca gaccgaatag tctctaaacg
     1441 ttcttcaaac attaatcacc tctttaattc ataatcaaac agccttgcta tacttctgag
     1501 aaatcttcgt taactgctca atatcagcca tcaaagcaat cttcttctgc gactcatatt
     1561 cactaacaag ttctttcata gtatcaagtt cttttttaag cttttgcttt aacttatcca
     1621 gtttaccgga ataatgctta gtcaaattag cttcagctaa ctgaagaatc tcttcatctg
     1681 ttttatcagt catcagaata aattgaccag aaccccttaa tgcttgtctg acatcaatta
     1741 accggaaccc ttcagggata ctttgaagaa ctttaggaac gtcttcaatc gctgtaccaa
     1801 aaccatcatt aaagtttcct tcataataga taaacgggtt tgacttaatc tcgtaagcaa
     1861 tatcatattc tgaaggtttc gaggtttctt cgaagttctt aacagcatcg taagcgacat
     1921 caagtctaag acgctgtgat tttaaagctg ggtaatattc acgctcaagc tcaaatagtg
     1981 cttcagtttt gcagatatca ggcatttctt gaggctcagg agacacaaag gaaattacct
     2041 ttggtgacat ctttagctct tgctcaagct caatgcgctt gtcatgctct tgacgctgaa
     2101 tagtatgtag cggatgttca ccctgtggtg accagtctcc gaagctatca acctttttac
     2161 cgttaatata taaatcgttt gtcattataa tttcctctaa gtgtggttat ttcttaatag
     2221 agcaccttca acgccaatac acttagaaga tgacttaacg atttattaaa gatgctctat
     2281 taagaaaagc cctaaacata atctgtgaag ggctttggag gtttgcttat aacgttaacg
     2341 aagtttcagg aaaactttct ttaaaagctc tgtttaaaaa ctcttaaaga aagccctaac
     2401 tcatgattag ggcaaagctg taaaggattc aataatgaat gaacaaagac tttttgagat
     2461 agcctctcag accaacctga aaggctagga cgaaaagcat tgcacctttc caactcagaa
     2521 tatccattca tcggattatt gaaagaaaaa ataagagaga attgctctcc cccatatata
     2581 caattatgtc atgctcaagg ttgtttgtca acagccttta acagattggt aacatttata
     2641 ctttaataga tgcacctgca atcataccaa tcaatacaaa acagataatc accaattcca
     2701 ttaatgcacc accttcgaat ccgtcaccag aatattatcg ctatacaaaa ttgctatagc
     2761 cgccttgaga tatttttcag ggctaacgct gtatttgttc gtttcttcga taataagacg
     2821 cttcatgttt gagtcggtaa caggaaacat aaaacgcggt tcaccagttt caatttcctg
     2881 aataaagtct tcaatttctt ctgagttttc agccattagc tttagtaatt tatcaaggtc
     2941 a
//
LOCUS       DAJTVJ010000276         2936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000276 DAJTVJ010000000
VERSION     DAJTVJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.280"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..262
                     /locus_tag="OO448_004430"
     CDS             35..262
                     /locus_tag="OO448_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6339432.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="OO448_004431"
     CDS             complement(263..637)
                     /locus_tag="OO448_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCR6339433.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OO448_004432"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="OO448_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="OO448_004433"
     CDS             complement(2097..2672)
                     /locus_tag="OO448_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6339434.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          761 a          695 c          687 g          793 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctcttat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattgc tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag atttgttgtc tatagctata
     2761 gtgactcttc tatgtagaca ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttc
//
LOCUS       DAJTVJ010000277         2815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000277 DAJTVJ010000000
VERSION     DAJTVJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.281"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..991
                     /locus_tag="OO448_004434"
                     /pseudo
     CDS             1..991
                     /locus_tag="OO448_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1007..1756
                     /locus_tag="OO448_004435"
     CDS             1007..1756
                     /locus_tag="OO448_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6339435.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1758..>2815
                     /locus_tag="OO448_004436"
     CDS             1758..>2815
                     /locus_tag="OO448_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339436.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKIT"
BASE COUNT          886 a          566 c          585 g          778 t
ORIGIN      
        1 taaggttaaa tacatacgta agtttggctc gtaatactta ctgggacgcc agcagtaatg
       61 tgaattattc attatcactt agccgcgatt ttgatatcgg gccattaaaa aacgtctccg
      121 cttctctaac atttagccga ataaactggg aagaagacaa ccaggatcaa ctgtacctaa
      181 atatttcgat tccctgggga accagtagaa cattgagcta tggtatgcaa cgaaatcagg
      241 ataacaagat ttcgcatact gcttcgtggt atgactcttc cgatcgaaat aattcctgga
      301 gcgtttctgc ttcaggcgac aatgatgaat tcaaagatat gaaagcgtca ctacgcgcca
      361 gttatcagca taataccgag aacggtcgtc tctatctctc cgggacatca cagcgagaca
      421 gttattattc tctgaatgcc agttggaatg gttcattcac ggcgactcgt cacggagccg
      481 ctttccatga ctatagtggt agtgctgact cgcgttttat gatcgacgca gatggtgcag
      541 aagatattcc gttgaacaat aaacgcgcgg taactaatcg gtatggcatc ggagttattc
      601 catcagtcag cagttacata acaacatcat taagcgttga tacccgaaat cttccagaaa
      661 atgtggatat cgaaaactcg gttatcacca ccaccttaac cgaaggtgct attggctacg
      721 ccaaacttga taccccaagg gctatcaaat catgggggtt attcgcctgg cagatggtag
      781 tcacccacca ctggggatta gcgtaaaaga taaaaccagc cataaagaat taggactggt
      841 tgctgatggc ggctttgtat acctcaacgg cattcaggat gacagcaaac ttactttacg
      901 ctggggtgat aaatcctgtt ttattcaacc acccaatagc agcaacttaa ccaccggaac
      961 ggttatttta ccgtgtatta gccaaaatta attcaggatg aaactaatgc gctacttaaa
     1021 cacaaagaat ctcattgcag caggagtatt actcgcctgc atgagcagca ttgcctgggg
     1081 tgccatcatc ccggaccgta cccgtatcat aatgaatgag tcagataaag gggaagcatt
     1141 aaaacttact aatcaaagca aaaatctacc ctatctggcg caaacatgga tagaagatac
     1201 gaaaggcaat aaatcacgtg actttattgt cacggttccc cctatggtac gtttaaatcc
     1261 cagtgagcaa attcaaataa gaatgataag tcaggaaaaa attgctcaac tgcctaatga
     1321 cagagaaacg ctcttctatt ttaacgtgcg agaaataccg ccaaaaacag ataaaaaaaa
     1381 tgtgatgcaa gtcactatgc agcacgcatt gaaactattc tggcgaccaa aagctattga
     1441 attagaagat gatggggtaa tgtcttacga aaaagttgaa atcatccgta gaaatgatgg
     1501 ttcgatccgc tttaacaata agatgcctta tcacgttacg ctgggttata tcggaacaaa
     1561 cggaatcaca atgttgccac aaacgcaaag tttaatggta accccttata gccatgcaga
     1621 tacgcagttt aaaaatgttc cgtcagcttt tcaggttggt tatatcaatg attttggtgg
     1681 attaagtttt tatgaaataa actgcccgac ggttaataac tcttgtaatg tttccgtagc
     1741 caaacgagat aaataatatg cgctacttat taattgtaat tatatttatt ataggtttta
     1801 gtgcattacc tgtatgggca atggactgct atgctgaaca tgaaggggga aataccgtag
     1861 tcattggtta cgtaccaaga atttctatcc ccagtaatgg taaaaaaggt gataagatct
     1921 ggcaaagcag tgagtatttt atgaatgtct tctgtaataa tgcattacct gccccatcac
     1981 ctggagaaga atacccatca gcctggacta atataatgat gtttttacca ggcggtcagg
     2041 acttttataa tcagaactcc tatatttttg gcgtaacata caatggagtt gattatgatt
     2101 cgactgcccc taatgctatc gcagcaccag aatgcattga tataaaagga gcgggtacct
     2161 ttaataatca ctataaaaac cccgctgtct gtagtggagg tcctgagcct caattatcag
     2221 taacctttcc agctcgcgta caactctata ttaaattagc aaaaaatgcc aatagagtta
     2281 ataaaaacct ggtgttacct gatgaatata tcgctctaga gtttaaaggt atgagtggcg
     2341 caggagctat agaagtagac aaaaatttga ccttcaggat tcgtggatta aataatattc
     2401 atgtccttga ctgctttgtt aatgtcgcac tggaaccagc tgatggcgtt gttgactttg
     2461 gtaaaataaa ttcccgaaca attaaaaata ccagcgttag tgagacgttt agtgtagtca
     2521 tgacaaaaga tcctggtgcg gcctgtactg agcaattcaa tattttaggg agctttttca
     2581 ctacggatat tttgagtgat tatagccatc tggatatggg taatggactg ctattgaaga
     2641 tatttcataa agatggaaca gcaacggaat ttaaccgctt ctcacaattt gcttcttttt
     2701 catcgtctag tgcaccttcg gtcaccgcac cattcagggc agaacttagt gcgaacccgg
     2761 cagagactgt tgttgaggga ccgtttagta aagacgtaat cctcaaaatc accta
//
LOCUS       DAJTVJ010000278         2787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000278 DAJTVJ010000000
VERSION     DAJTVJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.282"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..411
                     /locus_tag="OO448_004437"
                     /pseudo
     CDS             1..411
                     /locus_tag="OO448_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            996..1712
                     /locus_tag="OO448_004438"
     CDS             996..1712
                     /locus_tag="OO448_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339437.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYILARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2264..2668
                     /locus_tag="OO448_004439"
     CDS             2264..2668
                     /locus_tag="OO448_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339438.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          808 a          533 c          611 g          835 t
ORIGIN      
        1 gaagacccag atatgaacag gcacattcag agttacttta aagagcttac tgaacgtctg
       61 aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat cgagcagcct
      121 ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg actcgggaac
      181 tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc tctgtacatg
      241 gcggcgcttg aggcaccccg gtttaatctg gtgataaagg cgttttacat gcgcctactt
      301 gcagccggaa acgccaaaaa agtggcactg gttgcctgta tgcgtaagtt gctgaccatt
      361 atgaacgcaa tgctcagaaa aaacgaagaa tggaatgaat cgtatttatg aaaactggat
      421 tgtgggccat aaatattcca aaaagatagc ccgctccgtt tattaccgga acgggcgaga
      481 gcatgacatt gtctgtaatt ataatgagac tgctgattct acttattttt aatttcaaga
      541 aaagcgtttt ctacaaagaa ttatgagatt taatgttttg tttagacaat attatcattc
      601 tgttcaataa tgaaagtctt catcaacaac aagtaaaaac aacgtccctc tcatcgaacc
      661 ggacttgcag atttcccgca tccggctctc ttgcaacaca tcagaccttc acgttcgggc
      721 ggttatgggg aaggtccagc aagctgtatg agccctaact gtttgaatac cagtagatta
      781 ccagaaactt ctatcgattg aagacattac caactagcgt tactaaatgt tgaccaagtt
      841 ttgttcactt gtctggtttt gatagtagca ttctgcagag tattttgata tgataaatat
      901 gcattaacaa tgatattatt gttaatttcc tagcatgaaa attaaaagat caacttttta
      961 tctcgaatat tttttacatt atcagttggt ttcttatgaa tgacaattca ttgcttagaa
     1021 attcttcatt attcatcgcc tatatgggat gtgtagggtg ggtcagtgct tattcttatg
     1081 gctggggaac ctctttttac tatggatttc catggtgggt tgttggtgca ggtttggatg
     1141 atgttgccag aagtttactc tatgctatca tagtaatggg tatacttttt accggttggg
     1201 ggattggtat tttatttttt cttcttataa agaaaagaag taaaatacaa gacttgagtt
     1261 tttttagact attttttgct attaccctac tgtttttccc tgtaatattc gaacttttaa
     1321 ttctcaaaca atatttcata cttccattat cactttcatg cattattagt tcattggtta
     1381 tttcaataat aattcgtata tatggacgga tcttttctgt atcatgtttt tcagatatcc
     1441 cttttgttag agaacaccgc attaagttaa ttatggctgg ttttttggtt tatttttggc
     1501 ttttttcctt tcttgttggc tggtataaac ctcaattaaa aaaagaatat cagatgcttt
     1561 gttataataa tagttggtat tatattcttg ctcgttatga ttcacggctc gttttgtcta
     1621 gttcatttaa agatgacagt aacagattct taatatttaa tacagaacag tcgggatttt
     1681 atgaaatcaa tgatgtttat gtcagaaagt aataatcctc tcccacaaag tgaattaaat
     1741 gaatatataa tgcgttcgaa gtatatgtat tgagctgtca tactgagaaa caaaccttag
     1801 ggcagcacaa ttaaagccag aaacaactat gcaagaaagt tactaaataa gtgctcggaa
     1861 cagtaaaaca ctcagggggg actcagtccg gattatgtaa tcagataaat tgccaaacat
     1921 cacaaatcac cagccggact gagtgatgcc gagcattgca ccaatacccc gtgatgaacg
     1981 atgcctgatg cagaaagctc cctataaaac aaagaacaaa aactacgacc ggcgattcac
     2041 cgtcatactg atgctgcatc gtggtgacac tgtctgtcac tttcatgttg actgaacaac
     2101 tctgctgcga agaaggtgta ccgccggcgg gatttgattg tccggcgtct agcttccact
     2161 tccctgatgg aactgaaagt tgttatggcc ggtcagcttg ccctgtccgc aggacatgag
     2221 gtgctgtgac ggacagcacc agtaacggaa agtgacaatg aaaatgaata ctgcgctgat
     2281 agttgcacta atgtgtatgt ggtatgcggt acccgctgct gcaaaagaga ctttactggc
     2341 tatgccacgt aattcaacag aacattgcta tgcggagatt aatgtgcacg gaccatatgg
     2401 ggtgtatttc cgagtggtcc cgcatccccc cgggggaaag agctgggtcg aatgtaacag
     2461 tgactactat tactctgata aaccaccggg agtacagata ctcggaaccc gtgcagggtg
     2521 tcgggtatac ggaatctgcg ggaccacctc gacactgcat gtggctggaa gaggggttgt
     2581 gtgtattaaa aatatatgca gtccacgtgg gatgataatt cacaggatac ggaaacgtcc
     2641 tgtagtggct gtaagcgatg agatgtgatc ttacccagca atagtggaca cgcggctaag
     2701 tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac cagcaaaaaa
     2761 acacgtaaac agcattcgcc tgaattt
//
LOCUS       DAJTVJ010000279         2741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000279 DAJTVJ010000000
VERSION     DAJTVJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.283"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1008)
                     /gene="shiA"
                     /locus_tag="OO448_004440"
     CDS             complement(<1..1008)
                     /gene="shiA"
                     /locus_tag="OO448_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCR6339439.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1323..2375
                     /locus_tag="OO448_004441"
     CDS             1323..2375
                     /locus_tag="OO448_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCR6339440.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          593 c          617 g          682 t
ORIGIN      
        1 cgccataaag aaaggaaatg cgctcaacgt tccgattaac gcacctgtga tataaaccct
       61 acggcgacca aaacgatcgg caagccaggc aaaacaggga attgtcaggc agcttaatcc
      121 acctaccagc aaaccaatat taaggaaaag ttcgcgcggt agccccatat tctgggttga
      181 ataattaagt gcaaaggcag taacgatgta catcgtcagc aattcgcaca gtcgtagcgc
      241 aataatcttc aggaaagcac cgggatgtcg taacagcgct tcgataaccg ggatgcgttt
      301 tttcgcggca gcttgataat gttgctgttg ttcaaattcc gcggactcct ccatgccatt
      361 gcgcacccac aatgctccca gtaccagtac gatgctaaac aggaaaggaa tgcgccagcc
      421 ccagcttaaa aactgttcgt cagtcgtcat catactgatc aatgaaacca gtccggttga
      481 aagcagtaaa cctacaccgt agccaacttg tacaccgcta ctgtaaaagg cttttttatt
      541 tttcggtgca ctttcaacgg aaagcaacgc cgcgcctccc cattcgccgc cgacggcaaa
      601 tccctgaatg acacgcagtg tcaccagtaa aataggtgcc caccacccaa tggtcgagaa
      661 tgaaggaaga ataccaatca aggctgtcgc gatgcccatc atccagacgg tcagcattaa
      721 catgcgctta cgtcccagtc ggtcgccaaa gtgaccgaaa atgacaccgc cgagcggacg
      781 gaaaaggaat ccgacgccaa aggtagcaaa tgcggcgagc gttcccatcg ccgggcttac
      841 ttgcgggaaa aactcgcgat taaacaccag tgcggcggtg atgccataga gtaaaaaatc
      901 ataccagtcg acgacggcac cagcgaagct gcctaacgca gctcgtcggg cgcgacttaa
      961 cgaaagcgtc ccttcatcgg gacgagtgga gatgagcgtg gagtccataa tgatcctgtt
     1021 tgtactgtct ttttataatt agtatggaaa aggtaactca cattgccgtc gatctataaa
     1081 cgaaccttat gagactacct cgacagagcg catctgcaaa tgattttatg gctgattagc
     1141 ggcaacatga aaaattcgat ggaagatgaa agcgcaggag ttgctggtgt tgtttaagga
     1201 ttaagcagag gacccacagg ttagagatat gaaacataaa agtccgcata tgcacaaaat
     1261 ggcaccctga tgagtctgat agactgcatt gcattataac cagtagggag gcgaggttag
     1321 gtatgttttt agcatcattg ttgagacgta ttgcatttag ttactacgat tataaagctt
     1381 ataatttcaa tattgaaaaa acagactttg ttgtcatcca tattcccgat cagattggcg
     1441 atgctatggc catctttcct gttattcggg cgcttgaatt gcataaaatt aagcatcttt
     1501 taattgtaac gtcgacaatt aatttagaag tctttaatgc gcttaaactt gaacagacta
     1561 aattaacatt agtcacaatg actatgcagg atcacgcaac attaaaagaa ataaaagatt
     1621 tagcaaagaa cataacacag caatacggta cgccggatct ttgtattgag gggatgcgta
     1681 aaaagaacct gaaaacgatg ttatttatca gtcagttgaa agcaaaaacg aattttcagg
     1741 ttgttggtat aaccatgaat tgctactccc ctttgtgcaa gaacgcgtcc cgtatggatc
     1801 agaaactccg ggctcccgta cctatgacat gggcatttat gatgcgtgag gcgggttttc
     1861 cagcagtcag gccaatatat gaattgccac taagtgagga tgtactcgat gaggtgcgcg
     1921 aggaaatgcg atcgttagga tcttacattg cgttcaattt agaaggtagc tcgcaggaac
     1981 gtacattttc attatcgatt gcagaaaatc taatagcaaa aattcaaagt gaaacagata
     2041 taccaatagt gatcgttcat ggacccaaag gtgaagataa agccagggca ttagtggatt
     2101 gttataataa tgtctaccgt ttatccttac caccctcgat taaacgttca gcagcaatca
     2161 taaaagatgc ttatatcgca ataactcctg acacctcaat attacatatg gcaagtgcct
     2221 ataatacccc tgttgttgca atttatgctg attacaaaac gcgatggccc gcaatggccg
     2281 atgtctcgga gtcagtcgtc gttgggcaaa aaattgacaa tataagtctg gatgaattcg
     2341 caaaggcatt aaaaagtgtt ttggcgagaa tatgagacag tacttatgca tagtaatgcc
     2401 gatctgttat gaacggtggt gtcatcaata ctctgaggct ggtcaatcat cacagaaaac
     2461 cagcctttga atcaaagcag tactcaccgg atacgatcat agtcacatcg aacgcaattc
     2521 tggcggtgcc gcatttttat ttattcattt tctgtgagag aagggcaagc tctatagagg
     2581 gaatgtaaaa aagagagctc cgaatggagc actctttaag aaattataac ggaaaatcag
     2641 aggtttttat aacatgtcgc ataagcaaac ccaagctttt caaatacgct ttgatcacct
     2701 gttgccaggg aaactacgta ttgttcgctt gagtttgcgg g
//
LOCUS       DAJTVJ010000280         2689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000280 DAJTVJ010000000
VERSION     DAJTVJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.284"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            230..769
                     /locus_tag="OO448_004442"
     CDS             230..769
                     /locus_tag="OO448_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6339441.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OO448_004443"
     CDS             complement(995..1318)
                     /locus_tag="OO448_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6339442.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OO448_004444"
     CDS             1604..1798
                     /locus_tag="OO448_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6339443.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          856 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataggcgct cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     1921 cgagcggtgt cagctcactc aaaggcggta atgcggttat ccacagaatc aggggataac
     1981 gccggaaaga acatgtgagc aaaaagccga aggcagggac aaaggccgcg caggtggcgt
     2041 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2101 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2161 gctctcctgt tccgaccctg ccgtttaccg gatacctgtc cgcctttctc ccttcgggaa
     2221 gcgtggcgct ttctcatagc tcacgctgtt ggtatctcgg cccggtgtag gtcgttcgct
     2281 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2341 actatcgtct tgagtccaac ccggtaagac acgaattatc gccactggca gcagccactg
     2401 ataaccgaat tagaggagta agacttgaag tcatgcgcct gttaaggcta aactgaaaga
     2461 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggctcaaaga gttggtagct
     2521 ctgagaacct tcgaaaaacc gccttgcagg gcggtttttt cgtttacagg cagcagatta
     2581 cgcgcagaaa aaaaggatct caagaagaac ctttaatttt ttttcacact tggtatccta
     2641 ctttaaccca taaaataaac acttacaccg acacaccctt aaacaaaag
//
LOCUS       DAJTVJ010000281         2641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000281 DAJTVJ010000000
VERSION     DAJTVJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.285"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..1165)
                     /locus_tag="OO448_004445"
                     /pseudo
     CDS             complement(1..1165)
                     /locus_tag="OO448_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            1154..1561
                     /locus_tag="OO448_004446"
     CDS             1154..1561
                     /locus_tag="OO448_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR6339444.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVE
                     GLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDAD
                     YDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            1699..2583
                     /locus_tag="OO448_004447"
     CDS             1699..2583
                     /locus_tag="OO448_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCR6339445.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
BASE COUNT          415 a          790 c          860 g          576 t
ORIGIN      
        1 ggtaggcttg cacgccctgc ggcacgtaca gcttggtttc gccgaggtcg cggatgcgcg
       61 gcgcgaagcg gaagcctagc aggtgcatca gggcaaagac gtgatcggtg aagccggccg
      121 tgtcggtgta gtgctcctcg atccgcaggt cggactcgtg gtacagcagg ccgtcgagca
      181 cataggtgga atcgcggacg ccgacattca ccacgcgggt gctgaacggc gcgtactggt
      241 cggagatatg ggtatagaac agccgtcccg gctcgctacc gtacttcggg ttgacgtgcc
      301 cggtgctctc gccccggcca cccgcgcgga agcgctggcc atcggaggat gaggtcgtgc
      361 cgtcgcccca gtgggcggca aaggcgtggc gatactggtg gttgaccagc tcggccaagg
      421 ccgccgaata ggtttcgtcg cggatgtgcc aggcttgcag ccaggacagc ttggcgtagg
      481 tcaggccggg gctcgactcg gccatcttgg tcagcccgag gttgatcgca tcaccgagga
      541 ttgcggacag cagcaacgtc ctgtctttgg cctcggcccc gtccttcaag tgggtgaagt
      601 ggcggctgaa gcccgtccag tcgtccacgt ccatcagcag ttcggtgatc ttgatgcgcg
      661 gcagtaactg gctggtttgg tcgatcagcg cctgcgcccg atccggcacc gccgcatcca
      721 gcggggtgat tttcagccct gactcggtga ggatggcatc gggcagctcg ttgtccttgg
      781 ccaggcgggt gacggtggcc aactgctcgt ccagcagctg caaacgctct tccaggtact
      841 ggtcgctgtt cgggttgatc gccaggggca gggcctgctc gcgcttgagt gcggcgaact
      901 tctcggccgg cagcaggtag tcgtcgaagt cgcggaactg ccgcgagccc ttgacccaga
      961 tgtcgccgga gcgcagggcg ttcttcagct cggacagggc gcagatttcg tagaatttcc
     1021 ggtcgaggcc ttccggggtg atcaccagcg gcttccagcg cggcttgatg aaggccgtgg
     1081 gtgcatcggc cggcaccttg cgcaggttgt cggcgttcat ctcacgcagg gtctgcacgg
     1141 ctgccagcac gccttgcgcg gccggcccgt gctgttcccc aacttttcgc tcggcgaaga
     1201 gtatgaacat gcgccgccgg cgacgaatcg ccaaatctca ccgtatctcc cgagcggacg
     1261 atttcgcacc ggtctgcccg tcgaagggct tgcgatcgaa cggggcgacc ttttctatgc
     1321 atgtccgcga gccagcgtct tttatggcac ggcgctcgac gccgaccttc ggacgcgcgg
     1381 cgtaagcacg cttgtcatgg ccgggataag caccaccggc gttgttcttt caagcgtcgc
     1441 ctgggctagt gatgcggact acgacgtgcg tttggtccag gactgctgct acgacccgga
     1501 tcgggatgcc cacgaagctc tgttgcgttc cgggttcggc ggacgtgtac aggtcgtgta
     1561 attttcgggc ctcgcatgat cgcggccata gccgcaggtg gattggtgcg aggcagggcc
     1621 agtccaagtc agggtgcggt catcgcggcg cctgcaactc tcaaaaaccc atcgccgtag
     1681 cggaggtctt gtaatcgcat gtcattacgc acgcccgatt tattgttcac agcgatagca
     1741 cctgccattt ggggcagcac ctacattgtc accacccaat acctgccgaa cttctcaccg
     1801 atgacggtcg cgatgctgcg ggcgttgccg gcgggtttat tgctcgtgat gatcgtccga
     1861 cagattccaa cgggaatctg gtggatgcgc atcttcatcc tcggcgcact taatatttcg
     1921 ctattctgga gcttgttgtt tatttcggtc taccgcctgc cgggcggggt cgcggcgacg
     1981 gtaggcgctg tgcagccgct gatggtcgtg ttcatctctg ccgctctgct aggtagcccg
     2041 atacgattga tggcggtcct gggggctatt tgcggaactg cgggcgtggc gctgttggtg
     2101 ttgacaccaa acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc gggggcggtt
     2161 tccatggcgt tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc tctgctcacc
     2221 tttaccgcct ggcaactggc ggccggagga cttctgctcg ttccagtagc tttagtgttt
     2281 gatccgccaa tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg gctcggcctg
     2341 atcggagcgg gtttaaccta cttcctttgg ttccggggga tctcgcgact cgaacctaca
     2401 gttgtttcct tactgggctt tctcagcccg gggaccgccg tgttgctagg atggttgttc
     2461 ttggatcaga cgctgagtgc gcttcaaatc atcggcgtcc tgctcgtgat cgggagtatc
     2521 tggctgggcc aacgttccaa ccgcactcct agggcgcgta tagcttgccg gaagtcgcct
     2581 tgacccgcat ggcataggcc tatcgtttcc acgatcagcg atcggctcgt tgccctgcgc
     2641 c
//
LOCUS       DAJTVJ010000282         2635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000282 DAJTVJ010000000
VERSION     DAJTVJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.286"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..454
                     /locus_tag="OO448_004448"
                     /pseudo
     CDS             1..454
                     /locus_tag="OO448_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            451..930
                     /locus_tag="OO448_004449"
     CDS             451..930
                     /locus_tag="OO448_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCR6339446.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(984..1934)
                     /gene="corA"
                     /locus_tag="OO448_004450"
     CDS             complement(984..1934)
                     /gene="corA"
                     /locus_tag="OO448_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6339447.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2304..>2635
                     /locus_tag="OO448_004451"
     CDS             2304..>2635
                     /locus_tag="OO448_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339448.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          780 a          595 c          565 g          695 t
ORIGIN      
        1 taaatttatc ttttcatgaa aaaataaata tggttataat agtccaatac gaataatcaa
       61 agaatgtatc gaaaatggca ttaattcaac tccctcgaca ggataccaac ctcttatcct
      121 gcaaagcatt aaaaccaaat tactttcatc aagactaaat aaatttaatg acttcgagga
      181 cagttttaat ggcttaggaa tcagtgttca tgatatttca gctcaaaaaa tatctctttt
      241 aagttttcaa aaatacgcta taggatggag tgcaacaatt cattttgtag cacaggacca
      301 cttcggtctt gatgtaactg atatcaaaaa caagacttac agcaaatata gattttttcg
      361 catatggttc tttttacaac ggcataaaga ctttgctttc aaaccttttt tcaccaattt
      421 taacactatc gaaagaatcg aaaattatct atgaaaagca aactccagaa aattatatta
      481 tgtttatttc ttctatgctg catctataat ttatggacat tgcgtcctgt acagattttg
      541 tatacatact ctgatgctgg caattcagtc tttctggtcg ttgatcatct gccatggact
      601 gacagcgata aaataaattg gtatctgaag catcagaatg aaattaaaaa tcaacatccc
      661 ctaccagaag gtagttggca tacctggtat gtcattgata tcggtaatgg cttcactgac
      721 tataaaaaat atatcgaagg gccgtatgaa gatctctact gtttcccgac aattaagagc
      781 aatgataatt gcatcgtgaa aaattactta atggttatta atgaataccc ttatcgtaac
      841 actcatattg gtattaacga ttttactgag tatcaattaa cacaagaaaa taaaatagag
      901 cgagttttca atccgcatga ctttaaataa gacaattttt agaaataagc cggataaaaa
      961 aatagcttta tccggcactc atattacagc cagttcttgc gcttaaagta cagatacggt
     1021 gccaggcccg cgaggatcat aaagataatc gcgcctgggt agccgaaact ccacttcagt
     1081 tctggcataa actcaaagtt catcccatag ctggaagcaa cgagcgtcgg cggcaggaat
     1141 accacggata ccaccgagaa gattttgatg atgcggttct gctcgatgtt gataaaaccc
     1201 atcgccgcct gcatcaggaa gttcaccttc tggaacaggg attcgttatg cggcagcagg
     1261 gattcgatat ctcgcaggat ttcacgcgcc tgctccagtt gcccacccgg taaacgcgct
     1321 ttacgcacca ggaagttgag cgcgcgctgg gtatccatca gacacaggcg aactttccag
     1381 ccgatatctt ccagttccgc cagagtggag agcgcctcgt cgtactcatc gccctgatgc
     1441 ccttccataa tcacccggct caactgctcc aggtcgctat agatattttc aatttcatct
     1501 gccaactgtt cgattttggt ttcgaacaga tccagcagca gctcgtaggc gttaccgtct
     1561 accatcgact ggctacgggc acgcatacga tacagacgaa aagcgggcag ttcacgctca
     1621 cgcagagtaa acagacgacc atcacggatg gtaaatgcca cagtggagtt accggcgtga
     1681 tcttccgcat cttcaaagaa gaagaaggag tgaatatgca ggccgtcgtc gtcttcaaag
     1741 aaacgtgccg atgcttcgat gtcttccagt tccgggcggg ttgccaggct ctggccaagt
     1801 tcagattgta cgcgcagtcg ctcgtcgtcg tccggttcga caagatcaat ccatactgca
     1861 tttacaaggg gttgtgactc ttcgacttcc agccgggtca gtcggttatt ttccagttga
     1921 aatgcgctca gcatgaccgg gactcccaat gcttaaaata tcggacagtt cggtgggcac
     1981 acagaaacaa attgggtttc agaccattaa acagcctgac tcagcgcgac gggaaaaatt
     2041 gaggtcgctg acaaccgcta aggctatcag caaaaaggga tagccttagg agttgatcct
     2101 ggatgacagg atagtgagcc agtatctact gggtgtgtcc aaggcgaatg tcctcttaga
     2161 gtgatcgtgc gcgcatgtta cgccagcaaa attttgccgt caacacgcaa cagaacacca
     2221 cagaacaata gttgaacttc gcgctttttg gtactcgtca aggtcgctaa caggataaaa
     2281 aaatccaaca tccaggacct ataatggcgc atcggctact cattggtaaa ggaatgatca
     2341 ccttgaatct caaacggatt tttctcgccc tcaccttact tcccttattt gctgttgccg
     2401 ctgatgattg cgcactctca gatccgatgc tgaccgtaca ggcgtatacc gttaatcctc
     2461 aaacagagcg ggtgaaaatg tactggcaaa aagccaatgg cgaagcgtgg ggaactttac
     2521 atgctctgct ggtggatatg aatagtcagg gtcaggtgca gatggcgatg aacggcggca
     2581 tctatgatga aagctatgcg ccgctcggtt tgtacatcga aaacggtcag cagaa
//
LOCUS       DAJTVJ010000283         2628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000283 DAJTVJ010000000
VERSION     DAJTVJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.287"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            31..2547
                     /locus_tag="OO448_004452"
     CDS             31..2547
                     /locus_tag="OO448_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6339449.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
BASE COUNT          752 a          641 c          597 g          638 t
ORIGIN      
        1 acaaatataa aaaacacagg tcatcaggga atgccacaac gacaccacca gggacataaa
       61 cgcacaccga aacagttggc gctcatcatc aaacgttgtt tgccgatggt gctcactggc
      121 agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc cattatgctg
      181 gaaactacaa aaagtggtat gcaaacaacc gatctgtcac gtttttcaaa aaaatacgca
      241 caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt ttcacagaaa
      301 aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac ggtagaacaa
      361 ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa agatgacgat
      421 agcgtgatca actcgcttga acaaatcatt cccggtacag cagctgaatt tgatttcaat
      481 catcagcgac ttaatttgag cattccccaa attgcactgt accgtgatgc aagaggttac
      541 gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta ctcgtttaca
      601 ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct gaatatgcaa
      661 aatggtgcta attttggccc ctggcgatta cgcaactatt ccacatggac acgcaacgat
      721 cagacatcaa gctggaatac catcagtagt tatttacaac gtgatatcaa ggcattgaag
      781 tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag ctacaccttt
      841 actggcgttc aactcgcttc cgacgataat atgttgccaa acagccagcg cggatttgcc
      901 ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca aaatggttat
      961 gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct ctacccctct
     1021 tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacgca acgtcgcttt
     1081 atccagcctt attcttcatt acccatgatg cagcgacctg ggcatctaaa atatagcgcg
     1141 accgctggac gctatcgcgc tgatgcaaac agtgatagca aagaacccga atttgctgaa
     1201 gccacggcaa tatatggttt gaataatact tttacgctgt atggtggcct gctcggttct
     1261 gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact gggcgcgttg
     1321 tcgatggata tcaacagagc tgacacccaa ttcgataacc agcactcttt tcatggctat
     1381 caatggcgta cgcagtacat caaagatatc ccggaaacca acaccaatat cgctgtcagc
     1441 tactatcgct ataccaacga tggctatttt agttttgatg aagccaatac ccgtaattgg
     1501 aactataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac aatatttgat
     1561 ggggtaagtc tgtatgcctc cggttcgcag caagactatt ggggcaataa cgataaaaac
     1621 aggaatatct cagttggggt ttccggtcag caatggggaa ttggttacag cctgaattat
     1681 caatacagtc gctacacgga tcaaaataat gaccgcgcac tctctttgaa tctcagtatt
     1741 ccgttagaac gctggttacc gcgtagccgg gtttcctatc agatgaccag ccagaaagat
     1801 cgcccaaccc aacatgaaat gcgtcttaat ggctcactgc tggatgatgg tcgcctgagc
     1861 tatagcctgg aacaaagtct ggatgacgat aacaaccata acagtagcct gaacgccagt
     1921 taccgttcac cttatggcac cttcagtgcc ggatacagct acggtaatga cagcagccaa
     1981 tacaattacg gcgttaccgg cggcgtggtt atccatactc atggcgtgac gctctcgcaa
     2041 tatctgggca acgcttttgc gcttatcgat gctaatgggg cttctggcgt gaggatacaa
     2101 aactatccgg ggattgctac tgatcccttt ggctatgcag tggttcctta tctcacgact
     2161 taccaggaaa accgtctctc ggtagatact acgcagctgc ccgataacgt cgatcttgag
     2221 caaacaacac agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc
     2281 aatatcggtt atcgcgtact tgttatagtc agcgatcgca acggtaaacc gttgcccttt
     2341 ggcgctcttg ccagcaacga tgagacgggg caacaaagta tcgtcgatga gggcggcata
     2401 ctatatctct ctgggatatc gagtaaatca caaagctgga ctgtacgctg gggaaatcag
     2461 gcagatcaac aatgtcagtt tgcttttagt acaccggatt cagaaccaac aacctctgta
     2521 ttacaaggca cggcgcagtg ccattaagga taaaaaaatg aaaagagcgc ctcttataac
     2581 aggacttttg ttgatatcca catcctgcgc ttatgcctcc tcaggagg
//
LOCUS       DAJTVJ010000284         2600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000284 DAJTVJ010000000
VERSION     DAJTVJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.288"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..676)
                     /gene="curA"
                     /locus_tag="OO448_004453"
     CDS             complement(<1..676)
                     /gene="curA"
                     /locus_tag="OO448_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6339450.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYEN"
     gene            857..1375
                     /gene="mddA"
                     /locus_tag="OO448_004454"
     CDS             857..1375
                     /gene="mddA"
                     /locus_tag="OO448_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6339451.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1372..1821
                     /locus_tag="OO448_004455"
     CDS             1372..1821
                     /locus_tag="OO448_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6339452.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1822..2055)
                     /locus_tag="OO448_004456"
     CDS             complement(1822..2055)
                     /locus_tag="OO448_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6339453.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2141..2314)
                     /gene="ortT"
                     /locus_tag="OO448_004457"
     CDS             complement(2141..2314)
                     /gene="ortT"
                     /locus_tag="OO448_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6339454.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2509..>2600
                     /gene="yncL"
                     /locus_tag="OO448_004458"
     CDS             2509..>2600
                     /gene="yncL"
                     /locus_tag="OO448_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6339455.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          616 a          666 c          634 g          684 t
ORIGIN      
        1 cgttttcata atagatatca ataccttttg ggcacgcttt caccagttgt tcggcaaaat
       61 catccgcgtg gtgatcaaga caaacatcga agcccagcac ctcgatagca tggcggcatt
      121 tttccgcgcc accggcaacc cccaccaccc tgcaaccttt aagtttgccg atttgcccca
      181 ccgttgctcc cacaggacct gtcgccgcag ctaccaccaa cgtttcgccc tctttaggct
      241 gaccgatatc cagcagcccc atataagcgg taaagcctgg catccccagc acacccagcg
      301 accacgatgg attttgcgga tgatcgccaa gtttcaccag atcatcacca ctggatatgt
      361 catagtcctg ccatccactg tagcccagca cccagtcgct aggctgataa tcaggatgat
      421 tcgactccac aacacggctc accgtgccgc cgaccatcac gccgccaata tcaacaggcg
      481 gtgaataaga tggctcatcg ctcatacgtc cacgcatata cgggtccagg gacaaataaa
      541 ctgtgcgcag taacacctgt ccttcacccg gtgtggcgac atcatcttct tcaagacgga
      601 aattctccgg aaccggtgcg ccatgtggac gcgaggccag aacccaacgt cgattacgct
      661 gcttttgttg ccccataatg ctctcctgtg cttttggcat gaatccatca atagtagcag
      721 gctcacggga ttggttttgt ttgcagccgt cacatcccct ctgcaacacg caataatttc
      781 atcgaggaat ccgttgcttc gggctgtttt cgatagtatt gataacatca tcatcattat
      841 tttatcgagg ccagacatgt ccatccgttt tgcccgcaaa gccgattgtg ctgccattgc
      901 ggaaatttat aaccacgccg tgttgtatac ggcggctatc tggaatgacc aaacggttga
      961 tgcagataac cgcattgcct ggtttgaagc gcggaccata gcaggttatc cggtgctggt
     1021 aagtgaagaa gatggcgtag tgacgggata tgcctcgttt ggcgactggc gtagtttcga
     1081 tggttttcgc cataccgtgg aacattcggt ttatgtccat cccgatcatc agggcaaagg
     1141 tctggggcgt aaattgttaa gccgattgat tgatgaagcg cgggattgcg ggaagcatgt
     1201 catggtcgcc gggatcgaat cgcaaaatca ggcctcgctg catctgcacc attcgctggg
     1261 atttgtcgtc accgcacaaa tgccgcaggt gggcactaaa tttgggcgct ggctggatct
     1321 gacatttatg cagttgcaac tcgacgaacg cactgaaccg gacgcgattg gatgaatcag
     1381 tcgctcaccc ttgctttttt gattgccgcc ggaattggtc tggtagtcca gaacacgctg
     1441 atggtgcgca tcacccagac ctcctctacc attctcatcg ccatgttgct gaactcactg
     1501 gtggggattg tgctctttgt ttctatttta tggtttaaac aaggcatggc ggggtttggc
     1561 gaactggtgt ccagcgtgcg ctggtggaca cttatacccg gcttgctggg atcgtttttt
     1621 gtcttcgcca gtatcagcgg atatcagaat gtgggagcgg cgacaactat tgcagtactg
     1681 gtcgccagcc agctaattgg cggtctgatg ctggatatct tccgcagtca tggcgtacca
     1741 ctacgggcgt tgttcggccc aatttgtggc gcaattttac ttgtggttgg cgcctggttg
     1801 gtagccagac gctcatttta atcagaggat ggtgccgcct ttggttaact gttcgtggcg
     1861 cgcttccata tcttctttat gcttgcgacc gtgatgagca atagcggtgc gcaaacgctg
     1921 ctgctgggtg tagcgatctt cccgacttaa ctccgcgtca tcgctcaggg cgattaataa
     1981 ttcgttcata tgggcaatga cgctctcttc aatcgcggca tccacgcgcg cgatgacttc
     2041 atccagatgc gacatacttt ctccttaacc acttgtgaaa tttatcgatt catccaaatg
     2101 atataagtct gcgtggtgcc gtcaagccag tgagtaagat ttaaaacagt gaaaacaaca
     2161 gcgccaccgg gaaactcatc ggccatgttg cccccaccag aaaggcgctt aagaaacgga
     2221 tgcgtttctt atcgtgagaa agaaaccagg tgataagaaa tgcgattgct gccataaccg
     2281 cataaaaaac aagcatatgt tgatagagag acatgtacgg gcgtccagcc agatgaataa
     2341 aacgcgcgca aatatgcgtc attttttgac gtgcatcaag ttttttcacc agtttgagaa
     2401 aaaatcatca aacagcaact tgcccttatc catcttcaga atgagtatta ttgcggattt
     2461 tcttaacccg tactatacac ataggggcaa tgataaaagg tggcaaaaat gaatgtttcc
     2521 agtagaactg tagtactgat aaatgtcttt gctgctgttg gtttgtttac tcttatctct
     2581 atgagatttg gctggtttat
//
LOCUS       DAJTVJ010000285         2596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000285 DAJTVJ010000000
VERSION     DAJTVJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.289"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            355..1350
                     /gene="pgl"
                     /locus_tag="OO448_004459"
     CDS             355..1350
                     /gene="pgl"
                     /locus_tag="OO448_004459"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6339456.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1391..2344)
                     /locus_tag="OO448_004460"
     CDS             complement(1391..2344)
                     /locus_tag="OO448_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6339457.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          660 a          578 c          637 g          721 t
ORIGIN      
        1 agaataccga tctacaacgt aatgcgttta tttgtgccgg aagtgagttg attgttgagc
       61 gaatgggaag ttggccagaa gttgttatgg catagtgttt acggtcgttg atacaggtaa
      121 acaaaagcgg ggagagtcat actccctgaa tagtcgcgaa aatatctgtg gcattgtcaa
      181 attcgtgtcg gtgactaata ttttagtaat atgccattcg aaatggagtg ctaacttatt
      241 gatttaattg atgtgcgata ttgtatttta tcgtgttttg ttgtctgttt tttatccagt
      301 aagtgttgtt atgtgtatga aagataaggg atttatcatt tttgaggtgt gttcatgaag
      361 caaacagttt atatcgccag ccctgagagc cagcaaattc acgtctggaa cctgaatcat
      421 gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg ggcaggtgca gccgatggtg
      481 gtcagcccgg acaaacgtta tctctatgtt ggtgttcgcc ctgagtttcg cgtcctggcg
      541 tatcgtattg ccccggacga tggcgcactg acctttgccg cagagtctgc gctgccgggt
      601 agtccgacgc atatttccac cgatcaccag gggcagtttg tctttgtagg ttcttacaat
      661 gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc cagtgggcgt cgtcgatgtg
      721 gtcgaggggc tggacggttg ccattccgcc aatatctcac cggacaaccg tacgctgtgg
      781 gttccggcat taaagcagga tcgcatttgc ctgtttacgg tcagcgatga tggtcatctg
      841 gtggcgcagg accctgcgga agtgactacc gttgaagggg ccggcccgcg tcatatggta
      901 ttccatccaa acgaacaata tgcgtattgc gtcaatgagt taaacagctc agtggatgtc
      961 tgggaactga aagatccaca cggcaatatt gaatgtgtcc agacgctgga tatgatgccg
     1021 gaaaacttct ccgacacctg ttgggcggct gatattcata tcaccccgga tggtcgtcat
     1081 ttatacgcct gcgaccgtac cgccagtctg attaccgttt tcagcgtttc ggaagatggc
     1141 agcgtgttga gtaaagaagg cttccagcca acggaaaccc agccgcgcgg cttcaatgtt
     1201 gatcacagcg gcaagtatct gattgtcgcc gggcaaaaat ctcaccacat ctcggtatac
     1261 gaaattgttg gcgagcaggg gctactgcat gaaaaaggcc gctatgcggt cgggcaggga
     1321 ccaatgtggg tggtggttaa cgcacactaa ccgctgattt acccggcgca gtctctcctg
     1381 cgccggtgta ttaacctatc tcctgtaacg catgtctctg gcgttcgacg atattggtcc
     1441 acaaattgtc tttatcgtca gtccacaaat taatcagcaa ggcaaaaaag cgttctgctg
     1501 ccggagaaag cacggcatct ttgcggcgaa taatccccaa tgtccgacgt ataacgggtt
     1561 ccaccagtgg aataccaata atcgaagaat agggcgcatg gggcattgcc agaccaggaa
     1621 gcgccgaaat ccccagtccc gcctctacca gtcctaatga cgtcgaaaga tgacgcactt
     1681 cgtaaaacca gtccagcttc cagggcttgt cggccagctg ttgttctatc aacagtcggt
     1741 tgccgctgga ggagcgtacg ccaatcattt tgtaacccac taactcctgc cattctacaa
     1801 gctgcttttt ggccaatgga tggtcacgcc gacaggccag cacgaacggc tcgttgacca
     1861 gtggggtaaa atcaatggat gaatttgtga cgttgttcat gtttatgcca aagtctgatt
     1921 cgttacacag caccgattcc atgcaattat tcgtgccttg ttccagaatc cgcactttaa
     1981 tattgggata cagctcatta aatttaccga ttgccagcgg taaaaaataa aatactgcgg
     2041 tcggaataca cgctaacgtc accataccac gatgataagc attcatatca cgaatattaa
     2101 aaagcgtttc atcgaatttc tttattaatt cccgcgcctc cgggagcaac ctttttcctg
     2161 cttttgttaa cgtaaccttg cgagttgtac gctcaaatag ttgaatatgt aaatcttcct
     2221 ccatcttttt tattcttcgc gttaatgcag gttgcgtaat attgagtaat ttagcggcat
     2281 tgttaaacga actggactct gccagtatga caaatgcctt catacttgat aattcatgct
     2341 tcattattac tccggaaaat ggaagcgacg attttgggtg gctggccgtt aaaaatttta
     2401 actgcattta gccaacttaa attaatgaaa aaatgttatt aatcgttgag ctaaagtcat
     2461 tagagatgct ttgcccttaa tgtaaccata tcgcaataag ttatgttttt aaattgaggg
     2521 cattattatg aaaaaaatac cctgggtaat gactccaact tattgatagt gttttatgtt
     2581 cagataatgc ccgatg
//
LOCUS       DAJTVJ010000286         2576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000286 DAJTVJ010000000
VERSION     DAJTVJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.290"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2415
                     /locus_tag="OO448_004461"
                     /pseudo
     CDS             1..2415
                     /locus_tag="OO448_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            2417..2576
                     /locus_tag="OO448_004462"
                     /pseudo
     CDS             2417..2576
                     /locus_tag="OO448_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          699 a          536 c          730 g          611 t
ORIGIN      
        1 gccattttag gatatcaagc gaacagtaat ggtaaggtta tcgttgaaaa gggtggcgag
       61 tggctaataa aaaataatga ttcctcaatt gaatttcaaa ttggtaatca aggagctggg
      121 gaggcgacta ttcgcgaggg tgggctgatt acggctgaaa atacgattat cggtggcaat
      181 gccaccggta tcggaaccct gaatgtgcag gatcaagact ctgtcatcac ggtacgcaga
      241 ctctataatg ggtatttcgg taatggcaca gtcaatattt ccaataatgg tctgattaat
      301 aacaaagaat attcattggt gggcgttcag gacggttccc acggtgtcgt caacgtgacc
      361 gataaagggc attggagttt cctcggaaca ggcgaagctt tccgctatat ctatatcggt
      421 gatgctggtg acggtgaact taatgtctcg cgtgaaggta aagtagattc gggaattatc
      481 actgcgggga tgaaagaaac aggcacaggc aacattactg ttaaggataa gaactccgtt
      541 atcactaatc tcggaactaa tcttggttat gacggccacg gcgaaatgaa tatcagtaat
      601 gaggggcttg ttgtcagcaa cggaggaagc tcactcggtt atggagaaaa cggcgtcggg
      661 aatgtcagca tcaccacggg ggggatgtgg gaggtcaata agaatgtcta taccaccatt
      721 ggtgttgcgg gcgtcggaaa cctcaatatt agcgatggcg gtaagtttgt atcgcaaaat
      781 attacttttt tgggcgataa agcaagcggt atcggcacac tgaacctgat ggatgcgaca
      841 tcatcgttcg atactgtggg tatcaatgtc ggtaattttg gtagcggtat cgtaaatgtc
      901 agtaatggtg ccacccttaa ttcaacgggc tatggattta tcggaggaaa tgcctccggt
      961 aaggggatag ttaatatttc aacggacagt ctctggaatt taaagacatc atctactaac
     1021 gcacaattgc tacaggtcgg tgtattaggc acgggtgaac tgaatattac caccggaggt
     1081 atagttaaag cgcgtgatac acagatagct ctcaatgaca aaagtaaggg cgacgtgagg
     1141 gtggatgggc agaactctct tcttgaaaca ttcaatatgt acgtagggac atctggtacg
     1201 ggtacgttaa ccctgacgaa tagcggcacg ctgaatgtcg aaggtggaga agtttactta
     1261 ggtgtttttg aacctgctgt aggaacgcta aacattggtg cagctcacgg tgaggcggcg
     1321 gcagatgccg ggtatataac caacgcgacg aaagtagagt ttggttctgg cgaaggtgtt
     1381 tttgtcttta atcatactaa taacagtgat gccggctacc aggtcgatat gctgattaca
     1441 ggtgacgata aagacggaaa agtgatccat gatgcaggcc atacggtgtt caatgcaggg
     1501 aatacttata gcggtaaaac gctggtcaat gacggcctcc tgaccattgc gtctcatacg
     1561 gcagatgggg taacgggcat gggttcgagt gaagtcacca ttgcaagccc cggtacgctc
     1621 gacattctcg catcaacgaa cagtgcagga gattacacgc tgaccaatgc gctcaaaggc
     1681 gatggcttga tgcgagtgca gctgtcatcc tacgacaaga tgtttggctt tacccatgca
     1741 acagggactg aattcgccgg tgttgcccaa ctgaaagaca gaaccttcac tctggaacgc
     1801 gacaacaccg ctgcgcttac tcacgcgatg ttgcagtctg acagtgaaaa taccacatcg
     1861 gtaaacgtgg gagagcaatc cattggtgga ctggccatga atggcggtac gctcattttc
     1921 gatacggata ttcctgctgc gacgcttgca gagggatata tcagcgtcga tacgctggtt
     1981 gtcggcgcgg gtgactacac ctggaaaggc cgtaactatc aggtaaacgg gacgggcgac
     2041 gtgcttatcg acgtgcctaa accgtggaat gatcccatag cgaataaccc tctgacgacg
     2101 ctcaatttgc tggaacacga tgatagccat gtcggcgttc aactggtgaa ggcgcaaacg
     2161 gttattgggt cgggtggctc attaacgtta cgtgatttac agggcgacga ggtggaagcg
     2221 gacaaaacgt tacacattgc gcaaaacgga acggtggtcg ccgagggtga ttatggattc
     2281 cgcctcacga ccgcaccagg tgatggtttg tacgttaact atgggctgaa agcgctgaac
     2341 atccatggtg ggcaaaagct gacgttagcc gaacatggcg gagcctatgg cgcaacggcc
     2401 gatatgtcgg caaaatagcc atcgcttaca cgaccgtctg ggtgagccgc agtatacaga
     2461 ttcactgcat tctcagggat cggcaagcag tatgtggatg cgtcatgtcg gggggcacga
     2521 acgtttcagg accggtgacg gccagctaaa tactcaggct aaccgctatg tattgc
//
LOCUS       DAJTVJ010000287         2546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000287 DAJTVJ010000000
VERSION     DAJTVJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.291"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="OO448_004463"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="OO448_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6339458.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="OO448_004464"
     CDS             complement(390..1178)
                     /locus_tag="OO448_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6339459.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="OO448_004465"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="OO448_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6339460.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="OO448_004466"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="OO448_004466"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6339461.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2546)
                     /gene="pagP"
                     /locus_tag="OO448_004467"
     CDS             complement(2092..>2546)
                     /gene="pagP"
                     /locus_tag="OO448_004467"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6339462.1"
                     /translation="NIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGF
                     GQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTA
                     RDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          649 a          656 c          579 g          662 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tatttt
//
LOCUS       DAJTVJ010000288         2519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000288 DAJTVJ010000000
VERSION     DAJTVJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.292"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1112)
                     /locus_tag="OO448_004468"
     CDS             complement(<1..1112)
                     /locus_tag="OO448_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6339463.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETR"
     gene            complement(1177..1693)
                     /locus_tag="OO448_004469"
                     /pseudo
     CDS             complement(1177..1693)
                     /locus_tag="OO448_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            complement(1764..>2519)
                     /locus_tag="OO448_004470"
     CDS             complement(1764..>2519)
                     /locus_tag="OO448_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCR6339464.1"
                     /translation="TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFL
                     KRLTAPTITSGGNPSAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGML
                     EATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGI
                     AYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTL
                     EFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          491 a          733 c          651 g          644 t
ORIGIN      
        1 cgggtttccg acgctttcgc gttcgtttcg gatgtcttcg ctgcggaagc tgacctcgct
       61 gctgccgtgg cctgctcagt ggcttcgcca gccttcgttg tggctgttga agcggatgat
      121 gcggcgcttt ctgccgattt tccggcggcg gtggcactgg ctgaggcctg cccggcactt
      181 gttgacgcgg cgctggcaga cgacgcagcc gctgtttttg agcctgccgc agctgaggca
      241 ctctgtcccg ctgccgtttc agaagacctg gcgttcgtct cggacgtttt ggccgccttc
      301 gcagaatttg cagccgccgt cgccgaggaa gctgcgctac tggcgcttga tgatgcgttc
      361 gtttctgatg attttgccgc ctcttttgaa gccgacgcat cccgggctga ggtggcagct
      421 tctgacgctt tcgtggtcgc ggtggatgca gaagtggctg ctgattgttg tgacgctgcc
      481 gcattcgttt ctaacgtttt cgccgcaccg gcactggtag ccgccgcgct ttttgaggac
      541 tctgcagcgg cagcactttt tgaggcttca gtggcctttg ttgatgccgt tcctgcgctg
      601 gaagacgctg actgagccga cgaagcggcc tgtccggctg acgtgctggc tgcacgtgct
      661 gaatctgcag catcagtcgc atgggttgcc gcctcacggg ctgatgtgct ggcatcgctg
      721 gctgatttct tcgcggctgc cgtgttctgt gccaccgcgg acgcgttacg cgccacctct
      781 tccaccatca gctcaaaacg gcgcagtgcc tccggacggg catcatcctc cgtcatggca
      841 ccgagaaaat cattcagcgt accgggtcgg gaatcttcat acacggtgat ggtcccggca
      901 tgtgacggcg ggaatccttc caccaacaga ataacgctgt actgaccgta ctcaacgtcc
      961 atgctgtaac gcccggcttc atccggattt tctgaggcca gcgtgttcac caccaccgtg
     1021 gtgctgttac gttttgcttt cagctggatt gtgcagttct gtaccggttt tcctgtgccg
     1081 tctttcagta cacctgaaat ctttactgcc atattcaccc cacaaaaaag cccgcctgaa
     1141 ccggcgggct gtcataacac tgtgttacct ggctaatcag aatttataac cgacacccac
     1201 gatgaaaccg tcagtgcgcc agagacgtgt tgctgtggcg accgtcatca cttccggtca
     1261 gcacatcgtg cgttttcccc ttattgtcag ttacgcggat ataatccccg gaaaaagtcg
     1321 acacacggct gtaagccaca cccgccatcg catacgcgct gaaccattca ttcacgcgca
     1381 cagacggccc cgccatcacg ctgaaccagc ggttacgcac ggaatcttca tgccagcggg
     1441 tatcgctgta acgggtcagc tggcgattct tgtctcctgc atagctgaat gacgtcacca
     1501 gccccagcgt atccgtaaac tcataacggt atttcacgtt aatcccgttc agatcatcac
     1561 tgccgggaac gttggtccgg gcatgaagat accccgcgct cagcgtggac tgatgttcag
     1621 acgcccatgc aggcgcaccg gatacggcca ggcaaatggc tgcggacaaa atggctgcac
     1681 aaactttacg cataattacc tctcgctttt ctgcaataaa aaaggcgtca ttcctgacgc
     1741 catttattgg ggttataaat atttcaacga atactgatgc cggaagccgc ttttttggtc
     1801 acaatcaccg tacagtcggt gatattgcct gccccctgat tgcctttctg gaaaatctta
     1861 aactccagag tgacgctacc accaccacta ggcatatcaa taactgcact gtaactaccg
     1921 ggaatggcac ctttagtttc tctggatgcg attaatacgc cgtttttgcg aacttcaaaa
     1981 ccataacccg tgtatcgcgt gcctcctggg ttatttccgc tccccggatc gtcatacgct
     2041 ataccgttaa aaataatggg cggaataata atctggcggt caaagttatg atcatcgctt
     2101 atggtgactg taaccgtccc gtttggtgtt tccgtgttac cccacgtacc agcctgtttc
     2161 ggaaatgatt tggatacagc tttaacgaag tcacctctga cctgagtcgc ctccagcatg
     2221 cccttaatcg tacagttttc atttaccgtg acattgttga gcgtcccggc gttcgcattc
     2281 acactgccac tgatatccgc atttttagcg gtcagctttc cgtccggagt cagggaaaat
     2341 gccgatggat ttccaccgct ggtaatggtg ggggccgtca ggcgtttcag gaacacgtcg
     2401 ttcatgaata tctgatcgcc ctgaccaaca aacatcggtt ttgtgttgcc attcgcagga
     2461 ttaaccatcg caatcctgtc cgccgccagc agcacctgac tctgcatgcc gtcaggggt
//
LOCUS       DAJTVJ010000289         2481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000289 DAJTVJ010000000
VERSION     DAJTVJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.293"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..66
                     /gene="hokD"
                     /locus_tag="OO448_004471"
     CDS             <1..66
                     /gene="hokD"
                     /locus_tag="OO448_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6339465.1"
                     /translation="EVRIRTGQTEVAVFTAYEPEE"
     gene            370..642
                     /locus_tag="OO448_004472"
     CDS             370..642
                     /locus_tag="OO448_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339466.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            716..1702
                     /locus_tag="OO448_004473"
     CDS             716..1702
                     /locus_tag="OO448_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6339467.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLHHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            1703..2083
                     /locus_tag="OO448_004474"
     CDS             1703..2083
                     /locus_tag="OO448_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6339468.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            2080..>2481
                     /locus_tag="OO448_004475"
     CDS             2080..>2481
                     /locus_tag="OO448_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6339469.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          644 a          589 c          681 g          567 t
ORIGIN      
        1 gaggtacgaa ttcgaaccgg ccagacggag gtcgctgtct tcacagctta cgaacctgag
       61 gagtaagaga ccaggcgggg gagaaatccc tcgccacctc tgatgggtca ggcatcctca
      121 atgcacccac acttaacccg cttcggcggg tttttgtttt tattttcaac gcgtttgaag
      181 ttttagatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg agaagaggga
      241 tggaccccga acaggggagt gctatttatc tggaaggatt ctgttgatga aaatcgaaga
      301 attacgtgaa atttttagtg aagatggcct ctatactgtg cgcgttgaga atggcgctat
      361 tgtcagccac tgccttccag actacacaat tctgataatt cagccgtctt tgccagcagg
      421 cacgggcggc gctctcacgc atttaaatct gattggtacc agcatccccc atgcactgaa
      481 gaacaggccg aatggctcat tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc
      541 cttagcctcg actaccgtca ctggataatc tccgtcaggc tcccttactc cgaacggcca
      601 acgcgtccgt cccgcacatt ccagcaacgg atctggaggt aatgtgcggg tattacttcg
      661 acctgttctg gtaccggaac tcggtctggt tatcgttaag ccaggccgtg aatcaatgtc
      721 agcattccat aacggcagaa tactggtgga gccggaacca aaaagcatgc gtaatctgcc
      781 gtccggggtc gttcctgccg ctcgccagcc gctggtggaa gacaaaacat tgctgccgtt
      841 tttcagtaac gcacgggtga ttcgtgctgc tggtggtgct ggtgcattgt ctgactggct
      901 gttgcaccat attaaatcct gccagtggcc acacggcgat tatcatcaca gcgaaaccgt
      961 cattcaccgt tatggtaccg gcgcaatggt gttgtgctgg cactgcgaca accagctgcg
     1021 tgaccagaca tccgaatcac tcgagcaact tgctcatcaa aacctgtcag catggatgat
     1081 tgacgtcatc ggtcacgcaa taagcggtac gcaggagcgt gaattatctc tggctgaatt
     1141 atcctggtgg gcggtccgca atcaggtggc ggacgcgcta ccggaagcgg tattacgtcg
     1201 ttcgctgggg ttgcgtgcgg aaaaaattcg ctctgtgtac agtgaaagcg acatcatacc
     1261 gggagagcag acagccacca gcatactgaa gcagcgcaca aaaaatattg cgttactgcc
     1321 tcacgtccac cagcaacaga acccaccaca ggaaaagacg gtggtcagca ttgccgttga
     1381 tccggagtca ccggctcagt atctccagcg ccagaaatca caacgggaag agatgcctgt
     1441 atacacgcgc tgggtaaaaa cgcagaaatg catgacgtgt ggcaatcagg cagatgatcc
     1501 gcatcacatc attggtcatg gactgggagg gatgggaaca aaggctgatg atttgtttgt
     1561 tattccgtta tgccgtaaat gccgtaacga actacatgcc ggagtaaaag attttgaaga
     1621 gaaacacggc agtcagctgt tgttgctgat tcgtttttta atgcacgcga gaaattcggg
     1681 tgttctgaag tggaaagcat aaatgaccga gcgcatagaa tttgttttac cttacccgcc
     1741 gacggtgaac acttactggc gtcgtcgtgg cagcacatat tttgtatcaa aagccgggga
     1801 gcgttatcgc cgggcagtgg cgcttattgt tcgccagcag cggctgaaat taagcctgtc
     1861 cggaaggttg gcaataaaaa ttattgcaga accaccggat aagcgccgcc gtgacctgga
     1921 caatattctg aaagcgccgc tggatgcgct gacgcatgcg gggttgctaa tggacgatga
     1981 gcagtttgat gaaatcaata tcgttcgtgc tcagccagta tctggtggac gtctgggggt
     2041 gaagatttat cccataatgc ttgaagggca ggtcaaaaaa tgaaactgga agatttaccg
     2101 aaatactact ccccaaaatc ccccggcctg actgatgcat cggcctcaac gtcgaaagat
     2161 gcgctgagta tcactgatgt gatggccgcg cagggcatga cacagaatcg ggctgagatg
     2221 ggggtttctg cgttccttgg gaaaatgggc attagtatga atgacagaga gcgggcaaca
     2281 gaattgctga cagaatatgc actcagtcgg tgtgatcgcg tggcggcgtt aagaaaactc
     2341 ccggcagaaa taaaaccggc agtgatgcgt attatggctt cgtatgcgtt tgaagattat
     2401 gcccgtagcg cggcgagcaa aaaacagtgc ccctgctgtc acggaaaaaa atttattgaa
     2461 agcgaggttt ttacaaacaa g
//
LOCUS       DAJTVJ010000290         2456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000290 DAJTVJ010000000
VERSION     DAJTVJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.294"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1859)
                     /locus_tag="OO448_004476"
     CDS             complement(<1..1859)
                     /locus_tag="OO448_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6339470.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDGGTYAITAVQHVPEKEAIVDNGAHFDGDQSATVNGVTPPA"
     gene            complement(1920..2447)
                     /locus_tag="OO448_004477"
     CDS             complement(1920..2447)
                     /locus_tag="OO448_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6339471.1"
                     /translation="MRALATQLPAFRQKLNDGWYQVRIAGRDAGETELSARLNEPLAN
                     GAVIHIVPRLAGAKSGGVFQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGG
                     VAQMLAPKARTSTAASTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEIS
                     TADEGDGGQVVVIGR"
BASE COUNT          485 a          810 c          626 g          535 t
ORIGIN      
        1 gctggcggcg tgacaccatt caccgtggcg ctctggtcac cgtcaaagtg cgccccgtta
       61 tccacgatgg cttctttttc cggtacatgc tgcacggcgg tgatggcata cgtgccgccg
      121 tcgttctcac ggatactcac gcagcggaac aggcgctggc gcagcgtcgg cagcttcagc
      181 ccccacacgc tgtattcagc aacaccgtca ggaacacggc tcactttcac cttcacgccg
      241 tcggtgacgg actgaacctc cacgctgacc ggattgccac ttccgtcaac caggcttatc
      301 agcgtggtac cggaggatgg cagcgtgatt tcacggtcga gcgtcagcgt ccgggtctgg
      361 ctgtttaccg ccagcacgcg cccgccggtg ctgataccgg catagtcatc atcgcagatt
      421 tcaatgacat cgcccggtac atggcgaagc ccttctgcgc cgacgctgaa atccacggtc
      481 tgcgtttcca gcagttctgt tttaatcagc cacagcccgg cgcggtgcgc ctgcccccgg
      541 ctggtacagc caaaggcatc catcttcgtg acgttacgac cgtaacgggc aatggcctgc
      601 gtatcttcaa caagctctgt cgccgtctcc cagccgttgt tcgggtcaat ccagttcacc
      661 tcaacggcat tatggcggtc cttcagggcg ctgaagctgt agcggaacgg cgcgccatca
      721 tccggcatca ccacattact gcggttatag gtccacacct tatctgatgg tcggtcctgc
      781 acgaacgtca gcgtctgccc gttccatacc ggcatacagc gcatcgccga gcagaaatca
      841 ctgagcacat cccacgcctt acgctgcgtg gtcaggtagg cattacaggt gatgcgcggc
      901 tccgtgccgc caaaaccgtc cggcaccgac tggtcgcagt actggccgat gacatacagc
      961 gcccatttat ccacatctgc cgcaccaaga cgtttcccca tgccgtagcg cggatgggtc
     1021 agcatatccc acagacacca ggccatgttg ttgctgtatg ctggcttaaa cgttccgtcc
     1081 cagataccgc tgtattgccg cgtctgcggg ttatagttcg acggcacctg cagaatgcgc
     1141 ccgcgcagat gataattacg gctcacctgc tggctgccga actgctccga gtccacctgc
     1201 acgccgacca gtgccgtgtt cgggtagcac tgtttcacat cgataatttc ggtgtatgac
     1261 gaccagagcg ttttgttctg cagctggtct gtggtgctgt ccggcgtcat cctgcgcatc
     1321 cggatattaa acgggcgcgg cggcaggtta tccaccacca ccgaggccag atactgtgaa
     1381 gtggttttgc ccttaatggt gatgtctttt tctgtcaccc agccaccgtt acgttgtatc
     1441 tgaaccagca ggcggacttc cgacggattc ctgtccccct ttgaggtggt ttccaccagt
     1501 gcctgcacac cgaaggtaaa gcgcagacgg tcgatatttg cagacgtgat ggtccgggtg
     1561 atcggcgtgt catatttcac ttccgtaccc agcaccgtct cggagccgga ggattcaaat
     1621 ccctccggcg gagtctgctc ctgctcacct gcccggaaca ccaccgtgac accggagatg
     1681 ttggtattcc cctcagtgtc cagcaccggc gtactgttca gcagcacgct ttttaatcca
     1741 tccaccggac cttcaatcgg cccttcactg atggcatcaa tcacactcag caactgcgtg
     1801 gatttcaggt tgtccttcgc ttcgcgcggg gtatgcccct tactgctgcc tttacccatt
     1861 cgtcatgctc cataaacgac aaaaccgccc ggaggcggtt tcacataaaa cattttgcat
     1921 cagcgaccaa tcaccacaac ctgaccaccg tccccttcgt ctgccgtgct gatctcctga
     1981 gaaaccaccc gcgaccccac acgcatttca ccgtacagaa caggcaaaac attgccctga
     2041 gcaaccatgt tatccagtga ggaaaaatag gtgttctgtt tgccgttatc tgtactggct
     2101 gccgtggacg tcctggcttt cggtgccagc atctgcgcca caccgcccag gatcatactg
     2161 gcccctgccg catacatgcc cgatacagcc gcggcaccca gccagcccac agggttccac
     2221 catgccaccg caatcaacgc cgcccccagc accacctgaa acacaccgcc acttttagct
     2281 cccgccagac gcggcacgat atggatcacg gcaccatttg ccagcggctc attaagacgg
     2341 gcagataatt cggtttcacc tgcatcacgc ccggcaatgc gcacctgata ccagccgtca
     2401 ttcagtttct gacgaaacgc cgggagctgt gtggccagcg cccggatggc ttcggc
//
LOCUS       DAJTVJ010000291         2299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000291 DAJTVJ010000000
VERSION     DAJTVJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.296"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..544)
                     /locus_tag="OO448_004478"
                     /pseudo
     CDS             complement(1..544)
                     /locus_tag="OO448_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(597..1193)
                     /locus_tag="OO448_004479"
     CDS             complement(597..1193)
                     /locus_tag="OO448_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6339472.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1220..1822)
                     /gene="yadL"
                     /locus_tag="OO448_004480"
     CDS             complement(1220..1822)
                     /gene="yadL"
                     /locus_tag="OO448_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCR6339473.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(1837..>2299)
                     /locus_tag="OO448_004481"
     CDS             complement(1837..>2299)
                     /locus_tag="OO448_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6339474.1"
                     /translation="TTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFK
                     LVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFL
                     KPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          633 a          509 c          457 g          700 t
ORIGIN      
        1 aactccaata taacacgtct tcgcttggat tataccacga aaaatattgt tctgcagagt
       61 caccaatgta tatcccaggt gaactaacgt tagttacggt accgtaagct gaccacatgt
      121 ttgaaattaa taatgtgtaa tacaaaccag gaactgatgt attaaataat ttatgggagc
      181 catacatttt acctgagtca accattgcat tttcaatacg cattgcgcta ccacttgtgc
      241 tatcacttct gttgcaataa agaaaaccac caccagaagc agtatctaac tgactctact
      301 gtaattttac acctgtaggt ccaagctgcg tcggtttgta gaaaactaaa ttatttgtac
      361 ttgttgcata tctgaatatg acagtagcat cgccattcgg ccccgtttct ctccattttt
      421 gatctgacgg accaacgccg taactattcc caacaatata tcctacgtta tcatcagtgc
      481 cagcactcgc ggtataacaa ctcaagcaca gaattaaaaa aaaatgtctg aagaatttct
      541 tcattatcac tccaccatct actgctgtaa cagtcgcagt tatttgtttt gatctattat
      601 tcgtagtaca catcaactaa tatatgactc attacttggc cagatatgac atccagcgcg
      661 ggatcgattt tttgcattct ggcataaaat ttccatgaag aattatgcag ggcagaagtc
      721 attactggat acacggaacg agaatttcct gctgaactaa taacattgaa tatattggtc
      781 gggtcctgaa tggaaaagag aacgacaccg acatttttag ccccggttgc gacttcattg
      841 gcaaatattt gccttgaacc tgcagccatg acaccgctaa gggctctaaa atcaatatgt
      901 aactgattaa gcacatcgcc agttccctgt aatgcacagt ctgatactgt gacagtgaac
      961 tcttttccac ctggttgata gctttcagga gtaacattat ctgaaaaata tcccacccca
     1021 acgacgccaa gatcaatatt accctggtta ctgattccgc ttttgcaagt gctgtttttt
     1081 acattggcaa caaggtcaat atcctgccca gcgatggctg gataggtaat gggaaactgt
     1141 agtgacagaa acagaattat tctcttcatc agcttacgtt gagtgggatg catatatagt
     1201 tcctggatct gtcagtctgt tagttatagg ttactgtaaa tgtggcaggt gcagaaaatt
     1261 tacccgcagt gacattgctt acggttttat ttttttcgac gaccagtcga gcactcatcg
     1321 gataataaac aaccgcgttg gctgcattgg agatagtgac ctcttgagat gctggtgtca
     1381 cacaactaaa ttgtgttgcg ctccctgttg ccggagtttc ggtgctccag acttcaacgg
     1441 cgacggcact tgctttatct gcggctgtgg atccatttgc gagccccgca ccgtcattag
     1501 cagtaccttc gcatgtggct cgcttggtta attttatttg tgctttttta ttggggatac
     1561 ccgcacagtc tttgaatttg agtttgaatg ttttaacttt ggtcttggca ttgatttcag
     1621 aaatatatac atcgccaaag tcaacaacag aaatgtcctg atcagagtca tttactaatg
     1681 tagcggtaca agtacctatt tctacggtag taatcacggt cagcccaatg ctgtctgttg
     1741 ccgcataaga gggtacgctg aacaatgagg cagccaaaac gacactgctg gataatccat
     1801 aacgtaaatt tttaaatgtc atcttaatta tccttcttat tgatagatga aattaaacgt
     1861 tgccagtgca cgaaaattcc ccggtacgcc ttgccctgca tctgtttcac gtagcgcaac
     1921 ggtcatttca agacccttat cgggctgcat ctcgtctgtg ctccagcgta tcttttccgc
     1981 actgttaggt ttaaggaaag tggcatcatc agtagtccgt tttttgaaac ccataccgat
     2041 attacttgtc gtcgaagatg aatcaccaga ctgcggtata ataagcttag gtgagcttga
     2101 tgatgcattt ccggtcagag tggtatcaat ccaactgatg ccactgctgc acccactagc
     2161 aaccagttta aaatcagcct gagattctgt cgttttattc gcgatcttat ccagacccat
     2221 cttagggatt ctcaatgtgt agttattatt gccatcattc gtgacgttat taccagtaag
     2281 tgtgatgtta caggttgtc
//
LOCUS       DAJTVJ010000292         2290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000292 DAJTVJ010000000
VERSION     DAJTVJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.297"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1369)
                     /gene="phrB"
                     /locus_tag="OO448_004482"
     CDS             complement(<1..1369)
                     /gene="phrB"
                     /locus_tag="OO448_004482"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6339475.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKE"
     gene            complement(1366..1875)
                     /locus_tag="OO448_004483"
     CDS             complement(1366..1875)
                     /locus_tag="OO448_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6339476.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          595 a          583 c          524 g          588 t
ORIGIN      
        1 cctctttgtg ctcgactatc ggttgcggat aatccagctt cacacctgct ttctgcgccc
       61 acttccacgg ctcatgcacc gatttccccg gtacattgcg tagttccggt agccactggc
      121 ggataaactc accttcgaga tcaaatttct cgccctgggt tgtcgggttg aaaatacgaa
      181 aatacggcgc tgcatcggtt ccggttgaag cggcccactg ccagccaccg ttattggctg
      241 ccaaatcacc atcaatcagc tgcgacatga aatatcgctc gccttcgcgc cagtcgatca
      301 ataaatcttt caccagaaaa ctggctgtaa tcatccgtag cctgttatgc atccagccag
      361 tgctgttaag ctgacgcata gcggcatcaa caatcgggta tcccgttttg ccttcctgcc
      421 aggcctgtaa atgtgcggga ttgctctgcc actgtacacg atccgtccag gcaataattg
      481 gacgatgttt acacaacgag gggtgatacg ttatcaggtg acggtaaaac tcgcgccaga
      541 tcagctcatt aagccagaca ctaccgaccc caccgtccag cgcctgcggc tgttcagcca
      601 acaagcgatg caagcactgg cgaggcgata acccgcccgt tgccaggctg gccgacaaac
      661 ggctggtgcc ttccactgcc ggaaaatctc gttgttgctc atattctccg gcaccgttct
      721 ggcaaaactg gcgtaattgc gcaatcgccg ctttttcttc caccggaaaa tgcgcagtat
      781 cgaaagactg acgaggataa ttcagcgtaa tggatggcga gggctctatc gatccgctac
      841 tacgaacttt tggcgcagcg acgcactccg gcatcccttc ccgcagccgt ttcagccagg
      901 cattcttaaa aggcgtaaag actttgtaca tctcgtgatt accggtcatc accgcgccag
      961 gcggcaggat cacgctgtca tcaaatcctt cacacaccac gttacgcagc gctctttcaa
     1021 cttccacatc ccgcgcccgc tcattcactt catactgata gttataaaac aggtgggtaa
     1081 cgctgttttc cgcgcacacc tgtttaacta tttcgacact ggcgacaaag tcatccactt
     1141 cacggaacaa taaaggaata cctttttccg caagcgctat ttgtagccca ttcagttgag
     1201 cattgataag ttcagcctga cgcggcgaca tgttatgcgt cgcccactgg cgtggtgtag
     1261 cgatatacaa cgccagcacg cgtgcagacg aattgcggca ggcggcagcc agtgcgagat
     1321 tatcgtgcag acgtaaatct tggcgaaacc agaccagatg ggtagtcata aaactcctga
     1381 atggcgcaag ttaatcacac gcggccaaag ttcgaaataa cgctgccctg aaagccaggc
     1441 gtcatgataa agcgccatat agtgtttgat acgcatcagc ggcgcaagaa gtggttgctc
     1501 gccacggcga taactgtcga taagcgcagc cagctgctgg cgttctgtgg aatcaatatg
     1561 cgggcgaaaa taaccctgaa cgtgcatcaa aacattggtg tgatcgcgcg ggttggcggg
     1621 gtgagaaagc aaaacaacta cgcgttggcg gtactggttg taatagtcat ccagattttg
     1681 ccactggtga atatcagcca cgaacgggcc aagtttgcgg tactccggct gagaatgcgc
     1741 cagaaagacg agtttatagc ggctgtgata atcgagaagc gccccgcgcg ttaagccatg
     1801 ttctttcagt cgatgtaact catgaagggc aaagatacgg attagggtgc tgtcatcaaa
     1861 tcgttgtaga ttcattactg ctcctgaacg gtataaagga gtaagtgtag acgatggtaa
     1921 atgaatttta tggggcagag tttttgcggg gaagtatttt gttggtaaaa atattatcaa
     1981 cttcatctga gtgagaatta ttctactatt tcccgagctg cattcatttt ttgtattttt
     2041 tatttttaga aaataacagt tctcaaagct taattacctc actattaatt gtatttcaaa
     2101 ctaacaatcg cactccattt tctcttttta aaaaacataa acactgttaa aacaaatact
     2161 tatttcgtta tcttaaatgc tatttctaca tcactcgtcc ttataatcaa attattctta
     2221 ttcatagcta tttttgcagg tttaaaatag cggctttcac caaagtcagc catgaaaatt
     2281 agactacaag
//
LOCUS       DAJTVJ010000293         2276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000293 DAJTVJ010000000
VERSION     DAJTVJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.298"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..325
                     /gene="casB"
                     /locus_tag="OO448_004484"
     CDS             <1..325
                     /gene="casB"
                     /locus_tag="OO448_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR6339477.1"
                     /translation="VSANVKAIDERQPFAAQLAAVMSKGRFTRLSAVKTPDELLRQLR
                     RAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRIRWALEYYQAGDAD
                     NEQNQ"
     gene            337..1392
                     /gene="cas7e"
                     /locus_tag="OO448_004485"
     CDS             337..1392
                     /gene="cas7e"
                     /locus_tag="OO448_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCR6339478.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            1403..2149
                     /gene="cas5e"
                     /locus_tag="OO448_004486"
     CDS             1403..2149
                     /gene="cas5e"
                     /locus_tag="OO448_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR6339479.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            2131..>2276
                     /gene="cas6e"
                     /locus_tag="OO448_004487"
     CDS             2131..>2276
                     /gene="cas6e"
                     /locus_tag="OO448_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6339480.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRR"
BASE COUNT          565 a          568 c          610 g          533 t
ORIGIN      
        1 agttagcgcc aatgttaaag ccattgatga acggcagcct tttgccgcgc aactggcggc
       61 ggtaatgtca aaggggcgtt ttactcgcct gtcagcagta aaaacaccgg atgaattact
      121 gcgccagtta cgccgcgccg tcagattatt aaacggttcc gtcaatcttg actccctggc
      181 agatggcatt ttccgctggt gccaggagag cgacgacctg ctcaatcatc accgccgcca
      241 gcagcggcca accgaattta tccgtatccg ctgggcgctg gaatattacc aggctggcga
      301 cgccgataac gaacaaaatc aataaaggac tatctcatga caacatttat tcagcttcat
      361 gtgttaaccg cttaccctgc tgccaacctt aaccgtgatg acaccggtgc gccaaaaact
      421 gtggtgctgg gtggagcaac gcgtctgcgc gtttcctcgc aaagtctgaa acgtgcgtgg
      481 cgcacttctg cactttttga acaggcactg gcgggccata ttggtattcg cagtgggcgt
      541 attgcgcgtg aggcggcaac tatcctgatt gagaaaggga ttgaagataa aaaagccatc
      601 gaatgggcgg caaaaattgc tgattatctt gggaaagcta aaaatgacaa aaaaccaaaa
      661 gatccgctca ctaacgccga aactgaacaa ttagttcata tcagcccggc agaatttgac
      721 gccgtaaaag cgcttgctca tcaactggca gaagaaaagc gcgcgccaaa agaggaagat
      781 ctcgcgttgt tacgtaaaga ccgtatagcg gtagatatcg ctatgtttgg tcgtatgctg
      841 gcgaataaac ctgagtttaa tgttgaagcc gcctgccagg tcgcgcatgc gtttggtgtc
      901 agtgaaacga ttgtcgaaga tgattttttc accgccgttg atgatttgcg ccaggcctct
      961 gaagatgccg gtgccgggca tctcggagaa accggattcg gttcagcact tttctacacc
     1021 tatatctgca ttgataaaga tctgctggta gaaaacctcg gcggtgacga agcgttagct
     1081 aatcagacca tacgcgcttt tacggaagcc gcgcttaaag tttccccaac cggtaaacaa
     1141 aatagctttg ccagccgtgc ctatgcttcc tgggcgctgg cagaaaaagg caccgatcaa
     1201 ccacgctctc tggcggcggc tttctatgaa cccattaatg gcacacgtca gttagatgtg
     1261 gcggtgcagc gtattacaac gcttcgcgaa aacatgaata cggtctacga acagaagacc
     1321 gaatgcgcaa gttttgacgt gatgaacaaa cagggaagca tgaaggacgt gctggacttt
     1381 atctgcgcgt aaggggcggc ttatgagcca atatctgatt tttcagcttc atgggccgat
     1441 ggcgtcctgg ggtgtcgatg ctccaggtga agttcgtcat acccatgaac ttccttcgcg
     1501 ctcagcattg ctggggctac tggctgccgg ggtagggatt cggcgtgatg ataccgaacg
     1561 cttaaacgcg tttaatcgcc actattcact ggtggtttgc gccagccgta acccgcgctg
     1621 ggcacgggat tatcacacgg tccagatgcc aaaagaggtg cgtaaagcac gttatttcag
     1681 ccgtcgtgaa gagttgagcg accctgagct tttgagcgcg attatctccc ggcgcgacta
     1741 ctacaccgat gcctggtgga tggtggcggt ggcaacaaca gccgatgcgc cttacagcct
     1801 cgaacagttg caggacggtt tacgccatcc agtttttccg ctttatctgg ggcgaaaaag
     1861 tcatccgctg gcattacctc ttgcgccgtt actgctcgaa ggcaacgcgt gtgatgcctt
     1921 atgtaatgca taccaacagt atcaggatca tttccataaa ctgaaagtct cactcccgaa
     1981 acttcaggat gaatgctggt gggaagggaa acacgacggc ctggtcgcga gcaaaatatt
     2041 acgccgccgg gatgttcctt taaatcgtca gcagtggctg tttggggaac gcaccgtcaa
     2101 tcaggggccg tggctcagca aggaggaacc atgtacctct caagaataac cttacatacc
     2161 ggccagcttt cgcctgcgca gttgctgcat ctggtggatc gcggtgaata tgtgatgcat
     2221 cagtggctgt gggatctttt ccctggcggc aaagaaaggc aatttcttta tcgtcg
//
LOCUS       DAJTVJ010000294         2261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000294 DAJTVJ010000000
VERSION     DAJTVJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.299"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..398
                     /locus_tag="OO448_004488"
     CDS             <1..398
                     /locus_tag="OO448_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6339481.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRASWEK
                     TVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            623..863
                     /locus_tag="OO448_004489"
                     /pseudo
     CDS             623..863
                     /locus_tag="OO448_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            971..2029
                     /locus_tag="OO448_004490"
     CDS             971..2029
                     /locus_tag="OO448_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR6339482.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            2070..2145
                     /locus_tag="OO448_004491"
     tRNA            2070..2145
                     /locus_tag="OO448_004491"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2103..2105,aa:Ile,seq:cat)
     gene            2153..2229
                     /locus_tag="OO448_004492"
     tRNA            2153..2229
                     /locus_tag="OO448_004492"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2187..2189,aa:Arg,seq:tcg)
BASE COUNT          562 a          498 c          669 g          532 t
ORIGIN      
        1 cgccggtatg ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa
       61 aagtcaggga ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca
      121 ccgcctgtaa ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac
      181 gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac
      241 catcaacgga ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat
      301 gggaaaaaac agttaagaaa ttttatgatg cgctggtgac ccggtttgat attgaagaag
      361 catgggctga gcggcagtta aaaaaggtaa ctaggtaaca aggttgattt ttccggaatc
      421 tgtggtaaat tcgtcataac gatgggcgtt ttatgcctga cgttagaaga gtttctacaa
      481 cccgccgccg agcgggtttt ttatgactga aatcgcgtca gtacagtaaa cgcgctggtg
      541 gcggtgaata cctgtctttc agcttgctgg ctttttcgac aagagttatt ggtgtgtcac
      601 gttaaccgga aaaggaaaag acatgctaaa acagcaggat atgacagaaa ccgccagagt
      661 ggtgtttaat gaattaagcg ttaccgaacc ggcgacagtc ggggagatag cgcagaatac
      721 ttacctttca cgcgaacgct gccagttaat actgacccag ctggttatgg cgggtctggc
      781 agactatcag ttcggttgtt acagacgcct tccgcagtga aggctttttt atttgtggta
      841 aatgggcggc tggtgggtgt taggggcacc caccagccat ctgctcatgc gttgggttca
      901 caagcaaacc tcaggcccac tgctttgcgc aaaagcagaa tgagcctatc agagacaggc
      961 ttaatgatcc atgcttaata ctgtaaaaat atccagttgt gagttaatca acgccgactg
     1021 cctggaattt atccggtcgt tacccgaaaa ttctgttgac ctgatagtca cggacccgcc
     1081 gtactttaaa gtgaagcctg agggctggga taaccagtgg aagggcgacg atgattacct
     1141 gaagtggctg gaccagtgtc tggcgcagtt ctggcgggtg ctgaaacctg ccggaagtct
     1201 ttacctgttc tgtggtcatc gcctggcatc tgatatcgaa atcatgatgc gtgaacgctt
     1261 cagtgtgctg aaccatatta tctgggcgaa gccgtccgga cgctggaacg gatgcaacaa
     1321 ggaaagcctg cgggcgtatt tccccgccac agagcgcatt ctgttcgcgg aacattatca
     1381 ggggccgtat cgtccgaaag atgccgggta tgcggcgaag ggcagtgcac tgaaacagca
     1441 tgtgatggcc ccgctgattt cttactttcg tgatgcgcga gctgccctgg ggataacggc
     1501 aaaacagatt gcagatgcca caggaaagaa aaacatggtg tcgcactggt tcagtgccgg
     1561 tcagtggcag ttgccgaatg aaagcgatta tctgaaatta caggcgctgt ttgcccgggt
     1621 ggcagaagag aagcatcggc gtggtgaact ggaaaagctc caccaccagc tggtggatac
     1681 gtatacctca ctgaaccggc agtatgcgga gctgctgagt gaatataaac atctgcggcg
     1741 gtattttggc gtgacggtgc aggtgccgta taccgatgtg tggacgcata aaccggtgca
     1801 gttctatccc gggaaacatc cgtgcgaaaa accggcagaa atgctgcagc agataatcag
     1861 cgctagcagt cgtccgggtg acctgattgc agattttttc atggggttgg gttcgacagt
     1921 gaaagcggca ctggcgctcg ggcgtcgtgc aattggcgtt gagctggaga ctgaacgttt
     1981 tgagcagacg gttcgggaag tacaggattt agtcagccag aacggatgat attgaagaat
     2041 taattacgcg tcgttattat gcggctcccg gccctttagc tcagtggtga gagcgagcga
     2101 ctcataatcg ccaggtcgct ggttcaaatc cagcaagggc caccatcaca taccgccatt
     2161 agctcatcag gaaagagcgc cagccttcga agctggctgc gcggggttca agtccccgat
     2221 ggcggtccat tatctgcatc atgcgttgtt agctcagccg g
//
LOCUS       DAJTVJ010000295         2260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000295 DAJTVJ010000000
VERSION     DAJTVJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.300"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..987
                     /locus_tag="OO448_004493"
     CDS             <1..987
                     /locus_tag="OO448_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCR6339483.1"
                     /translation="FESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEP
                     KPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALP
                     AFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNM
                     QDGVRIARDNPDSGVVVSIAGEGRPWNPGAITDGRVWGDIPDNSVQPGAGNGEPVTAE
                     VLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTELAVREIAGQERDRAAITER
                     EAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKEKTPGGD"
     gene            1007..1754
                     /gene="traX"
                     /locus_tag="OO448_004494"
                     /pseudo
     CDS             1007..1754
                     /gene="traX"
                     /locus_tag="OO448_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            1809..>2260
                     /locus_tag="OO448_004495"
     CDS             1809..>2260
                     /locus_tag="OO448_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6339484.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVT"
BASE COUNT          495 a          579 c          755 g          431 t
ORIGIN      
        1 tttgagtcag cctacgtggc cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat
       61 aaccgtcagg gctggacgga tgccatcaac aatgccgtac agaaaggaac tgcccacgat
      121 gtgtttgagc cgaaaccgga ccgggaggtc atgaatgcag agcggctgtt cagtacggcg
      181 cgggagctgc gggacgtggc ggcaggccgt gccgttctcc gtcaggcggg gctggccggg
      241 ggagacagtc ctgcacggtt tattgctccg ggacgtaaat atccgcaacc gtatgtggca
      301 ctgccggcgt ttgaccgtaa cggcaagtcc gccggtatct ggctgaaccc gctgaccacg
      361 gatgacggaa acgggctgcg gggattcagc ggtgaaggtc gggtaaaagg cagcggggat
      421 gcgcagttcg tggccctgca gggcagccgt aacggagaaa gcctgctggc tgataatatg
      481 caggatggcg tccggattgc ccgtgataat cctgacagtg gtgtggtggt aagcattgcc
      541 ggtgaaggcc gtccgtggaa tcccggtgcc atcaccgatg gccgcgtgtg gggggatatc
      601 ccggacaaca gcgtccagcc gggagccgga aatggcgaac cggtcacggc agaggtactg
      661 gcacagcggc aggctgaaga ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt
      721 gtccgtaaaa tggcagagaa caaacctgac ctgccggacg gcaaaacaga gctggctgtc
      781 agggaaattg ccgggcagga gcgtgaccgg gctgccataa ctgaacggga agccgcgctg
      841 ccggagagtg tgctgcgtga atcacaacgg gagcgggaag cggtccggga ggttgcccgg
      901 gaaaatctgc tgcaggagcg tctgcagcag atggagcggg atatggttcg tgacctgcag
      961 aaagagaaaa ccccgggcgg agactgatac aggaagacaa acactgatga caacggataa
     1021 cacgaacacg acacgtaacg attcactggc tgcacggacc gatacctggt tgcagtcatt
     1081 tctggtctgg tcacccggac agcgggatat catcaaaacg gtggcactgg tgctgatggt
     1141 actggaccac atcaatctga tattccagct gaagcaggaa tggatgtttc tggccgggcg
     1201 aggagccttt ccgctttttg ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat
     1261 ccggcaaccg gccattaacc ggctgtgggg atggggaatt attgcccagt tcgcatatta
     1321 tctggcaggc tttccctggt atgaggggaa tatcctgttt gcctttgcag tggcggcaca
     1381 ggtgctgacg tggtgtgaaa cgcgcagtgg gtggcgtaca gcagcggcca ttcttctgat
     1441 ggccctgttg ggggcctttg tccggcacca gttacggcat tgccgggctg ctgatgctgg
     1501 cagtcagcca ccgactgtac cgggcggaag acagagcgga acgtctggcg ctggtcgcct
     1561 gcctgctggc tgttattccg gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg
     1621 gtcttgtgat gacggtgctg accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc
     1681 gtttctggcc cggggatttt ttcccggtat tctacgcctg tcatctggct gtgctgggcg
     1741 ttctggcgct gtgacgggtg tggtatcttt gaccgtaaca ggatgattcg tcagaggcag
     1801 aacacagcat gacagagcag aagcgaccgg tactgacact gaagcggaaa acggaagggg
     1861 agacgcctgt ccgcagccgg aaaaccatca tcaatgtcac cacgccacca aagtggaagg
     1921 tgaaaaagca gaagctggcc gagaaagcag cccgggaagc agagctggcg gcaaaaaaag
     1981 cgcaggccag acaggcactg tccatttatc tgaacctgcc cacgctggat gaggccgtga
     2041 ataccctgaa accctggtgg ccgggattat ttgacggtga caccccccgg cttctggcct
     2101 gcggtatccg ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg ctctcgcata
     2161 aaaaactgcg cagggcgctg aaggccatca cccgttcaga aagctatctg tgtgccatga
     2221 aagccggtgc ctgccggtat gacacggaag ggtatgtgac
//
LOCUS       DAJTVJ010000296         2216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000296 DAJTVJ010000000
VERSION     DAJTVJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.301"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..316)
                     /locus_tag="OO448_004496"
     CDS             complement(26..316)
                     /locus_tag="OO448_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6339485.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(306..743)
                     /gene="ratA"
                     /locus_tag="OO448_004497"
     CDS             complement(306..743)
                     /gene="ratA"
                     /locus_tag="OO448_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6339486.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            914..1396
                     /gene="smpB"
                     /locus_tag="OO448_004498"
     CDS             914..1396
                     /gene="smpB"
                     /locus_tag="OO448_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6339487.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1611..1973
                     /gene="ssrA"
                     /locus_tag="OO448_004499"
     tmRNA           1611..1973
                     /gene="ssrA"
                     /locus_tag="OO448_004499"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          623 a          580 c          494 g          519 t
ORIGIN      
        1 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
       61 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
      121 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
      181 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
      241 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
      301 caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
      361 gacgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
      421 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
      481 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
      541 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
      601 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
      661 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
      721 gcggtccggc taatctgagg cataactatt tccatcaaca aaaatccaac aaataatatc
      781 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
      841 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
      901 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
      961 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
     1021 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
     1081 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
     1141 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
     1201 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
     1261 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
     1321 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
     1381 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
     1441 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
     1501 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
     1561 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
     1621 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     1681 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     1741 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     1801 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     1861 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     1921 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccacttttga
     1981 caggactgca accggacaga gactataaaa acagtcactt acggacactg accagacagt
     2041 aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt caaaaaagcc
     2101 tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt taacacgatg
     2161 cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc cagacc
//
LOCUS       DAJTVJ010000297         2205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000297 DAJTVJ010000000
VERSION     DAJTVJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.302"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="OO448_004500"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="OO448_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCR6339488.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="OO448_004501"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="OO448_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
BASE COUNT          603 a          466 c          577 g          559 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgttgt catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagtcc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggt
//
LOCUS       DAJTVJ010000298         2173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000298 DAJTVJ010000000
VERSION     DAJTVJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.303"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>2173
                     /locus_tag="OO448_004502"
     rRNA            <1..>2173
                     /locus_tag="OO448_004502"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          568 a          474 c          686 g          445 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc
      721 gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg agcgttctgt aagcctgcga
      781 aggtgtgctg tgaggcatgc tggaggtatc agaagtgcga atgctgacat aagtaacgat
      841 aaagcgggtg aaaagcccgc tcgccggaag accaagggtt cctgtccaac gttaatcggg
      901 gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa
      961 tattcctgta cttggtgtta ctgcgaaggg gggacggaga aggctatgtt ggccgggcga
     1021 cggttgtccc ggtttaagcg tgtaggctgg ttttccaggc aaatccggaa aatcaaggct
     1081 gaggcgtgat gacgaggcac tacggtgctg aagcaacaaa tgccctgctt ccaggaaaag
     1141 cctctaagca tcaggtaaca tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag
     1201 aataccaagg cgcttgagag aactcgggtg aaggaactag gcaaaatggt gccgtaactt
     1261 cgggagaagg cacgctgata tgtaggtgaa gtcccttgcg gatggagctg aaatcagtcg
     1321 aagataccag ctggctgcaa ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg
     1381 gacgtatacg gtgtgacgcc tgcccggtgc cggaaggtta attgatgggg tcagcgcaag
     1441 cgaagctctt gatcgaagcc ccggtaaacg gcggccgtaa ctataacggt cctaaggtag
     1501 cgaaattcct tgtcgggtaa gttccgacct gcacgaatgg cgtaatgatg gccaggctgt
     1561 ctccacccga gactcagtga aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag
     1621 acggaaagac cccgtgaacc tttactatag cttgacactg aacattgagc cttgatgtgt
     1681 aggataggtg ggaggctttg aagtgtggac gccagtctgc atggagccgg ccttgaaata
     1741 ccacccttta atgtttgatg ttctaacgtg gacccgtgat ccgggttgcg gacagtgtct
     1801 ggtgggtagt ttgactgggg cggtctcctc ctaaagagta acggaggagc acgaaggttg
     1861 gctaatcctg gtcggacatc aggaggttag tgcaatggca taagccagct tgactgcgag
     1921 cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat ccggtggttc tgaatggaag
     1981 ggccatcgct caacggataa aaggtactcc ggggataaca ggctgatacc gcccaagagt
     2041 tcatatcgac ggcggtgttt ggcacctcga tgtcggctca tcacatcctg gggctgaagt
     2101 aggtcccaag ggtatggctg ttcgccattt aaagtggtac gcgagctggg tttagaacgt
     2161 cgtgagacag ttc
//
LOCUS       DAJTVJ010000299         2157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000299 DAJTVJ010000000
VERSION     DAJTVJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.304"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(357..614)
                     /locus_tag="OO448_004503"
     CDS             complement(357..614)
                     /locus_tag="OO448_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339489.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(611..1108)
                     /locus_tag="OO448_004504"
     CDS             complement(611..1108)
                     /locus_tag="OO448_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6339490.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1310..1768)
                     /locus_tag="OO448_004505"
     CDS             complement(1310..1768)
                     /locus_tag="OO448_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6339491.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1765..>2157)
                     /locus_tag="OO448_004506"
     CDS             complement(1765..>2157)
                     /locus_tag="OO448_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6339492.1"
                     /translation="GVRHNPYKDIVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDL
                     ATVARQINPYIKVDIPETTRCALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRR
                     WTYAGGNQWKGLMTRREIEREVCLWGKQ"
BASE COUNT          481 a          564 c          498 g          614 t
ORIGIN      
        1 ccgtgtaggg tataagccat tatcaaagcc actctgtagg gaatggcttt tgtgatggca
       61 tcacttactc tttacgctgc tatcccactc atcccggaat tttgatgggt tattgaaacc
      121 ttctgctgac ataacaactc cttcaatgtt tggctgaaat tgggatgtct ttccatcagt
      181 ccgccaccac aaagaatctt ttttgccata aggcaggagg ttcatctttc agtggctgcc
      241 ggtgttattt ccccacttac tggcttgggt tgtttcgtgg tactgccgtt aactggtggc
      301 ccagaataaa ttccggtttc attatcaagc ccacccgtaa atgggctttg taatggctac
      361 ttcgcttttg cttccgctcg cttacgccgg cgctcttctt tcctctcggc ttttgccatg
      421 tccatgaatg cctgcatgat cgagttccgc atcatgtagc taacaaagtg atgattgaca
      481 cagccgttga ggcgcagctg ctcgccaaac tcatccaccg aggccaatgc ttccatcatg
      541 cccttctcgc ctttcatgaa ctctgagaag tcgcgccccg ctctggaggc gcattcaata
      601 acatgatcac tcatcccgga agcccgggga tcgtaatctg cagctggtta gccagggagt
      661 taatctcagc gaccaacact ggcttcgtat agcgccatgc tgccagccct tgtccgcaga
      721 agctcgccat atctttcttc tggtcaaatt catgacattt catgttgagc tgcgcactta
      781 agctgttgcg atgctgaagt tctccggtga agtagtcatc gaggacttta taggccgcgt
      841 acttgaaccc ggggtttaac caagccgcat aatcgtaagc aacaaacttc ccgccatatg
      901 ttccaccgtg tacaccgcgc tcagtaaaaa ccacagattc gtggtttttc tccagctcgg
      961 ctaagaactc tttggtctgc ttgtttcgca ggtagtggta aggcgattca gattcacttt
     1021 taccactggc tttccacata tcagtgaggc agatcatgcc atcttcaccg atacgaattg
     1081 gttgattgaa gagggttaat gatttcatag cgtgtaccta ctctttgaaa tgaacctttg
     1141 ccgcacagga aaccagccca ccgaggctcg ccagcactaa ctggtatcct caaaggccca
     1201 ttccaaaggg gcaggttcgg tgtaaaaaac atgcgttgcg gtacgcattt attgcaaaaa
     1261 gccccgcatc gcgaggctca ttaaatggac tttgtgattt gcaaaaaaat tatttcaggc
     1321 attgcgtcct gatgtattcc tgcaggtagt taacctgcgc ggttatcttg tcgattccac
     1381 ttcggagacg gtaataattg agttcagcat ctgctgtaag tcctgggctt tctccatcgc
     1441 ccatgccgct ggctccggtc gttgactttg cacaggtggc ggcgacttgc aggcgcttac
     1501 gcccagcaga aacatcagca cggagacttt cgatagtcgc gttagcatca gcaagctcct
     1561 ttatgtatct tgcgtcgagt tctgctacat cacgttgacg cttccgcatg tcagcgatgg
     1621 tggcgttcgc cttctccagt tcactggcct tgttatcgcg ctgctctttg taggcgattg
     1681 cgttatcacg gtaatgatta acagccaatg acaggcagac gatgatgcag ataaccagag
     1741 cggagataat cgcggttact ctgctcattg tttcccccac aaacagactt cacgctcaat
     1801 ctcacggcga gtcatcagtc ctttccattg attaccgcca gcgtatgtcc agcgccgtag
     1861 ctgatcacat gcgcctttaa tatcaccctg gtttattttg cgaagaagcg tcgatgttct
     1921 gaaattgcca gcacccacgt tgtagacgaa cgagtaaaga gcgcagcgcg ttgtttccgg
     1981 tatatcgact ttgatgtacg ggttaatttg tctggcgaca gtggcaaggt ctttattcag
     2041 gagggctttg cactctgctt cggtatacgt tttaccggga atgatgtctt ttcctgtatg
     2101 cccgtaacat acagtccata caccaactat gtctttgtaa ggattatgtc tcacacc
//
LOCUS       DAJTVJ010000300         2124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000300 DAJTVJ010000000
VERSION     DAJTVJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.305"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1484
                     /gene="tssI"
                     /locus_tag="OO448_004507"
     CDS             <1..1484
                     /gene="tssI"
                     /locus_tag="OO448_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6339493.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1552..2111
                     /locus_tag="OO448_004508"
                     /pseudo
     CDS             1552..2111
                     /locus_tag="OO448_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          541 c          680 g          396 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcagtaacc
     1021 agataattaa agtgggttct gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg
     1081 tgggcgtggc gtatcagacg acggtaggtg gcattatgaa cacctcggtg gcactgatgc
     1141 agtcctcgca gatcggtttg cataaatcgt tgagggtcgg gctgggttat gacgtcaaag
     1201 tcggaaataa cgttaccttc accgttggta aaacgaaaaa ggatgatacc gggcagaccg
     1261 cgatttactc cgccggtgag catctggagc tctgctgtgg taaggcaagg ctggtgctga
     1321 cgaaggacgg acaaattttt ctcaacggca caaaaattca tttgcagggt aaggagcagg
     1381 ttaatggtga ctcactgttg attaactgga actgtgcagc ctcgaaatcc ccaccgaaga
     1441 cccctgatga aaagcaggat acgccggata tgagagagta ctgatcccgt acctgttcgg
     1501 tattcagtaa caaaccagca ttaaaaataa acagaagaag gaatttgaat catgagcgga
     1561 aaaccagcgg cgcgtcaggg agatatgact cagtatggcg gtcccattgt ccagggttcg
     1621 gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg
     1681 acttcgggca acccggtaaa tccgctgctg ggggcgaagg tgctgcccgg cgagacggac
     1741 cttgcgctgc ccggcccgct gccgttcatt ctctcccgca cctacagcag ctaccggacg
     1801 aagacgcctg caccggtggg cgttttcggc cccggctgga aagcgccttc tgatatccgc
     1861 ttacagctac gtgatgacgg actgatactc aacgacaacg gcgggcggag cattcacttt
     1921 gagccgctgc tgccggggga ggcggtgtac agccgcagtg agtcaatgtg gctggtgcgc
     1981 ggtggtaagg cagcacagcc ggacggccat acgctggcgc ggctgtgggg ggcgctgcgc
     2041 cggatatccg gttaagcccg catctttacc tggcgaccaa cagcgcacag gggccgtggt
     2101 ggatactggg ggggggctga gcgg
//
LOCUS       DAJTVJ010000301         2095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000301 DAJTVJ010000000
VERSION     DAJTVJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.306"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..285
                     /locus_tag="OO448_004509"
                     /pseudo
     CDS             1..285
                     /locus_tag="OO448_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            283..414
                     /locus_tag="OO448_004510"
                     /pseudo
     CDS             283..414
                     /locus_tag="OO448_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013183226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(508..981)
                     /locus_tag="OO448_004511"
                     /pseudo
     CDS             complement(508..981)
                     /locus_tag="OO448_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1154..1936
                     /locus_tag="OO448_004512"
                     /pseudo
     CDS             1154..1936
                     /locus_tag="OO448_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          486 a          582 c          540 g          487 t
ORIGIN      
        1 gcctccacag tacctgtcgt acttactgaa agtaccgtta tgcccgaagt ccccggggta
       61 aaaaagcgtc cccggcgccc caacttccct tatgagttca aaatcgcctt agtggagcag
      121 tcactgcagc ccggagcctg tgtggcgcag attgcccgtg aaaatggaat caacgataac
      181 ctgctcttca actggcgcca tcaataccgg aaaggtggcc tgctgccttc cggaaaaaat
      241 atgccggcac tgcttcccgt gacgttaacg ccggagccgg ataaccatgg ttttgacatc
      301 atctatatgc tttcgacaca tcatcagcgg ttcactttcg ttcgtctctt cgatccatac
      361 ctgatcggct cacgtccgac cttttcccat ctcgctcacc accacatctc ttaaatgcag
      421 cagcagtggg tggtttggtg cctgcgcctg cacgccgaca ccgaggggcc ttccctcatc
      481 tcttgtgcag catggctgca cagggactca cgtccctggt gcgcgccttc gtggcacacc
      541 atcatcggcg tacctttcga agggaacgtc gggacactga cgttgcatcc acatatcaaa
      601 ggcatagtgc aggaacagat ttgagagcag gggcgagatc acgccgccct gcggcgttcc
      661 ttctgtcacc ggccttacca gacctgcgtc cattacccct gcattcagga acctgcggat
      721 aagtgacaac acccgtttat ccgatactcg tttctctatc cggctcatca gcacgtcgtg
      781 atttatccgg tcgaagaact tctccagatc gaggtcgacc acccagtgat acccggcccc
      841 gatatattcc tgggcctgta tcactgcctg gtgggccgaa cgaccgggac ggaacccgta
      901 gctgttgtcg ctgaacgagc tatctcacag tgcctgcaat acctgcatca tagcctgctg
      961 gatgaagcga tccaccacca tctggatgcc cagcagacgt tcgccgccgc cgggcttcag
     1021 tatggatact cttctcacag gggatgggcg gtatctgccg gacagcagtt gcgctttcag
     1081 ctctggccag tggtgcctca ggtagtccgg cagttcactt acgcgcatgc catcgactcc
     1141 cgccgcacct tgattgcgtc ttgtcgatgg aacagcaatc agcgcgcccg ggggcggcag
     1201 cgctgaatgg cgactacata tgggatatga tcctcatacc tgtcagttca ctgattttga
     1261 gctaaccgac agcagagacg ctgaacggct ggatcgattt gcgcaaacgg cagacgagat
     1321 acgaattgct gaccggggat tcggttcgcg tcccgaatgt atccgctcac ttgcttttgg
     1381 agaagctgat tatatcgtcc gggtttactg gcgaggattg cgctggttaa ctgcagaagg
     1441 aatgcgcttt gacatgatgg attttctgcg cgggctggat tgcggtaaga acggtgaaac
     1501 cactgtaatg ataggcaatt caggtaataa aaaagccgga gctccctttc ctgcacgtct
     1561 cattgccgta tcacttcctc ccgaaaaagc attaatcagt aaaacccgac tgctcagcga
     1621 gaatcgtcga aaaggacgag tagttcaggc ggaaacgctg gaagcagcgg gccatgtgct
     1681 attgctaaca tcattatcgg aagatgaata ttcagcagag caagtggctg attgttaccg
     1741 tctgcgatgg caaattgaac tggcttttaa gcggctcaaa agtttgctgc acttggatgc
     1801 tttgcgtgca aaggaacttg aactcgcgaa agcgtggata tttgctaatc tactcgccgc
     1861 atttttaatt gacgacataa tccagccatc gctggatttt ccccccagaa gtgccggatc
     1921 cgaaaagaag aactaacgcg ttgtggagaa taacaaaaat ggtcatctgg agcttacagg
     1981 tggccattcg tgggacagta tccctgacag cctacaaaac gcaattgaag aacgcgaggc
     2041 atcgtcttaa cgaggcaccg aggcgtcgcg ttcttcagat ggttcaaccc ttaag
//
LOCUS       DAJTVJ010000302         2094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000302 DAJTVJ010000000
VERSION     DAJTVJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.307"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..124)
                     /locus_tag="OO448_004513"
     CDS             complement(<1..124)
                     /locus_tag="OO448_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6339494.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            385..720
                     /gene="iraM"
                     /locus_tag="OO448_004514"
     CDS             385..720
                     /gene="iraM"
                     /locus_tag="OO448_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6339495.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1001..1132)
                     /locus_tag="OO448_004515"
     CDS             complement(1001..1132)
                     /locus_tag="OO448_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6339496.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1632..1708)
                     /locus_tag="OO448_004516"
     tRNA            complement(1632..1708)
                     /locus_tag="OO448_004516"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1672..1674),aa:Arg,seq:tct)
     gene            complement(1809..1884)
                     /locus_tag="OO448_004517"
     tRNA            complement(1809..1884)
                     /locus_tag="OO448_004517"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1849..1851),aa:Ile,seq:cat)
     gene            complement(1987..>2094)
                     /locus_tag="OO448_004518"
     CDS             complement(1987..>2094)
                     /locus_tag="OO448_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6339497.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          522 c          370 g          525 t
ORIGIN      
        1 tataaggggt tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg
       61 tcagcttttc cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg
      121 ccatggttct ttccaccatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga
      181 aaaaacacaa aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa
      241 actacaagac gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa
      301 ataattctgc tctatggttt acattcaaaa atatcattta tacttttcag aacatcacca
      361 gcaaggcata aacaaggaaa ctaaatgaag tggattgtga ttgatacagt tatccagcca
      421 tcatgcggaa tatctttttc agtcatatgg agtaaaataa aattaataat ctggtatcaa
      481 tcggatgctt tcttacctcc tgaaagtata tttacactga ctcacacagg catcatgctc
      541 aataacaaag tgctacctgt aaccatttac aacgtagtac cattcaataa aacattctgg
      601 aattcaatca aaaatagcca ggaatgccct acaaatacag ataacgtatt gaatgaatgc
      661 tttaataacc gttgcactct gcaaatatgt ccttatggac taaaacaaca aagtccataa
      721 ggagtttact cacatctgac aaaatcaata taaacagccc ctccggagag gggctggaga
      781 gtggcgctat gtgccattgc atggtgccgg gtgcctcccg gtgaattcag taccagcacc
      841 tgaatccgcg attatcccat atacctactc gctgattgcc cctccgcaca gggggattca
      901 ccatgccagt ttcttttaac aaactccccg caaaccagac aacagtcaac cgcctgaatt
      961 gtgaagtatt taaaaatttc tcccgctaac tgatacccgg ctaacagtct ggcgttttct
     1021 ttttcagcaa cgggaaagca acaaccacca cacccgccac cagcacaccg tcagccagca
     1081 ctgacattat ccggctgctg caatgccatt cacaaaaaca gtaagcaatc actttttacc
     1141 gtaacaggtg ataatccaga tatgtatcta ccccagatga gtaatccgaa gttcatccat
     1201 accacaggtc ctggctattc tgttgtactc ctgaacaaga gcaaataatt ctgaattagc
     1261 aaccatgaac tcatcgcaaa ccctctgtat agcatcacta ttcagaataa taacgtctct
     1321 tcccgaaaga cgatcaggag tacagaacaa aactgtcaaa cggctgaagg cctttgctcg
     1381 tgctgcattg actatatcaa tacgctgcct aaggatgaaa caccccgacg cctcatcaat
     1441 attcactcta cccacaccat atgaatgata aatatttaat gctgaaaaaa ccattagacc
     1501 gtataacaaa cactcaatca atacttaaca gaacttttat ttttgacaaa cataaaatat
     1561 tttcaacaat atcctgagcc aggtatattt cagtataagg ctctgccgga aggaatctgg
     1621 aagaatgaat atggcgcgct gtactggatt cgaaccagtg accgattgct tagaaggcaa
     1681 ttgctctgtc cggctgagct aacaacgctg aataccgata atggaccgcc atcggggacc
     1741 cgcccccgca ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg
     1801 gtttgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg
     1861 ctctcaccac tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaattccgc
     1921 aataaaaaac ccgctcggcg gcgggttgta gaaactcttc taacgtcagg cataaaacgc
     1981 ccatagttat gacgaattta ccacagattc cggaaaaatc aacctagtta ccttttttaa
     2041 ctgccgctca gcccatgctt cttcaatatc aaaccgagtc accagcgcat cata
//
LOCUS       DAJTVJ010000303         2089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000303 DAJTVJ010000000
VERSION     DAJTVJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.309"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(60..1025)
                     /locus_tag="OO448_004519"
     CDS             complement(60..1025)
                     /locus_tag="OO448_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6339498.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="OO448_004520"
     CDS             complement(1879..>2089)
                     /locus_tag="OO448_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339499.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAJTVJ010000304         1887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000304 DAJTVJ010000000
VERSION     DAJTVJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.310"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..389
                     /locus_tag="OO448_004521"
     CDS             <1..389
                     /locus_tag="OO448_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339500.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO448_004522"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO448_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6339501.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OO448_004523"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OO448_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6339502.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OO448_004524"
     tRNA            complement(1806..1881)
                     /locus_tag="OO448_004524"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
BASE COUNT          503 a          446 c          457 g          481 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattag
//
LOCUS       DAJTVJ010000305         1871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000305 DAJTVJ010000000
VERSION     DAJTVJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.311"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(89..643)
                     /locus_tag="OO448_004525"
     CDS             complement(89..643)
                     /locus_tag="OO448_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6339503.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="OO448_004526"
     CDS             complement(640..930)
                     /locus_tag="OO448_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6339504.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="OO448_004527"
     CDS             complement(930..1529)
                     /locus_tag="OO448_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6339505.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="OO448_004528"
     CDS             complement(1601..1813)
                     /locus_tag="OO448_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339506.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          465 a          496 c          382 g          528 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat g
//
LOCUS       DAJTVJ010000306         1867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000306 DAJTVJ010000000
VERSION     DAJTVJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.312"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..926)
                     /gene="ospC4"
                     /locus_tag="OO448_004529"
     CDS             complement(<1..926)
                     /gene="ospC4"
                     /locus_tag="OO448_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6339507.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNK"
     gene            1342..>1867
                     /locus_tag="OO448_004530"
     CDS             1342..>1867
                     /locus_tag="OO448_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6339508.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWR"
BASE COUNT          546 a          367 c          351 g          603 t
ORIGIN      
        1 tttttgttat aacaaaactc tctgaatcct tgatctttac tctttcttat aaaatcaata
       61 agatgcaacc caattccaag cctcatatct ttagtgttaa atattggaac atcagttttc
      121 ccctgattac catgcacata cctgctaact tcatccacaa cctctttaaa gttatctaag
      181 aaaaatgatt ggtgctcatg gtaaaaaaca ggtggaatag cattatcaaa gtgatcggtt
      241 aatgtcatat atccatatgg agagtcatga tcaatgatat acgcattggc gccaaaatct
      301 acagtatgat gtgttttatt tagtgggagt ttttcttgat gattggaaat ttcaacccca
      361 aaaaaaacaa aatcgtggtt agagatttct ttaatatcct cagagtatgt attcttatgt
      421 gtttgtatat tttcagactg aattttatca aaggatttaa tattaagaat attatttgac
      481 agattagagt tactttgatg tctgaagtta aaattagaat taataataat agaaagtaag
      541 tgacgttcgt ctgaagaaag aggatgaact ttttgtatta tctcagagat atagtgtctg
      601 gacaccttat cgagatgctc aagtgaccga aatgatgacc gtgctccaga tggcgcttca
      661 atagaaagat ttagtgattt gaaactggtc ccttgagaaa acattttgct ataactttgc
      721 gctgctatag acttccttaa aaagtttttc acagtattag cacagtgatc taaatgtttt
      781 tcacttatgc cagaagaaga tgagtttgtt acgcgtaata tatttttttg taatgctttt
      841 gttgctttat tcggattcgt ttttccatca acaagatcaa tattattctg aacattaata
      901 tgatttactg cttcaggtat tttcatcccc cccccaatta tgtgcgtttt tatctgtggt
      961 tatttagata acattatcta actggacact ttcttgtatg tattatagct aaatgaacag
     1021 attttaatat gttgtatggt tttcactctg aaatcccctg aatttaatca cgtttccttt
     1081 actgaaggca attttaaccg tagtatcaat tattaaccct tgggcttgat atccctctcc
     1141 tcttctgggc acgggggatg tcagattact tattacagaa accaaaagaa ttgtaagcgt
     1201 catctcagtg tcgtctattt caatgtctga tgattggcga tgatagagtc ctcttataag
     1261 taagcgcccc atcagcgacg tcttgtgaaa attgtcctgt ctggcaacaa tcgcgcccat
     1321 ctatattgat ggacacgaac gatgaattcc cagacaacaa aagatattcc ctgcttccgt
     1381 tcttatttgc ctgatgccct gcgtttaaga tttgaagata aactgaccat ccgggccatc
     1441 gctcagcgtc tgggtctcag tcattccaca atacatacgc tttttcagcg atttcttgca
     1501 tccggtatcg catggccatt gcccgattca gtttcattcg ctcaacttga cgccatcctt
     1561 tatgccaaca gaaagaagga attaacagag cctcaaatca gagaaggctc atggcgaaaa
     1621 gaacggcgaa ccagctatag ccgtgaattt aaggtccgtc tggctaagca ggcattacag
     1681 cctggtgctg ttgttgcccg gatcgccaga gaacacgata tcaatgataa cctgctgttt
     1741 aaatggaaaa gccagtacga ggacggctta ctgagcgatg atgacataca ggaatgcatg
     1801 cctgtcccgg tggcactgac tgatacgccg gagccgacca gaccagttac aaatcccttc
     1861 tggcgta
//
LOCUS       DAJTVJ010000307         1851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000307 DAJTVJ010000000
VERSION     DAJTVJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.313"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(58..660)
                     /locus_tag="OO448_004531"
     CDS             complement(58..660)
                     /locus_tag="OO448_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6339509.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(597..1340)
                     /locus_tag="OO448_004532"
     CDS             complement(597..1340)
                     /locus_tag="OO448_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6339510.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            complement(1345..>1851)
                     /locus_tag="OO448_004533"
     CDS             complement(1345..>1851)
                     /locus_tag="OO448_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6339511.1"
                     /translation="GFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGTVVRRKV
                     YARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDGAVFPGR
                     IMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNTGNFGGF
                     LSINKLSQ"
BASE COUNT          415 a          617 c          445 g          374 t
ORIGIN      
        1 tcacgctcca taaacgacaa aaccgcccgc aggcggtttc acataaaaca ttttgcatca
       61 gcgaccaatc accacaacct gaccaccatc cccttcgtct gccgtgctga tctcctgaga
      121 aaccacacgt gaccccacgc gcatttcacc gtacagaacg ggcagaacat tgccctgggc
      181 aaccatgtta tccagtgacg agaaatacgt gttctgctta ccgttatccg ttgtctgtgt
      241 acggggagtt ctggctttcg gtgccagcat ctgtgcaaca ccgccaagcg tcatactggc
      301 accgagagaa aacagcagat tactcgccat aattcctacc cccggcatcc atatagcaac
      361 cgccataaca gccgccccca gcaccgcctg aaacacaccg ccacttttag ctcccaccag
      421 acgcggtact atgtggatca ctgcaccatt tgccagcggc tcattaagac gggcagacaa
      481 ttcggtttca cctgcatcac gcccggcaat gcgcacctga taccagccgt cactcagttt
      541 ctgacgaaac gccgggagct gtgtggccag tgcccggatg gcttcagccc ccgttttcac
      601 acgaaggtcg atgcggcgac caaatcgttg taaatccccg taaaggcaga tgcgcgccat
      661 gcccggtgac gccagaggga gtgtgtgcgt cgctgccatt tgtcggtgta cctctctcgt
      721 ttgctcagtt gttcaggaat atggtgcagc agcccgccgt caccgcagta aatggcggca
      781 tgattcggca ccgatgaacc aaaacagcac agcagcacat cgcccggctg tgcctctgtc
      841 agtgcgacac ggtaaaaacc cgtcacctcc atattgtcaa gatagagatt ctgaccgtta
      901 cgccaccagt catccccgcg atgaaaatcc ggcatctcaa tccctgccag atgataagca
      961 tcccggaaca gcgtgtaaca gtccgtcacc ccgtgctcaa agcgccgccc ggtgagatgc
     1021 ggcacacagc ggaacttgtg aatcgccccc cggcagacca gccaccacgg caaatcactc
     1081 tgcacctgca gccgccggtc agcctcactc agccagggca gaccaccggg atgactgtgg
     1141 accagcgcca caatctcacc ctgcatttgt gcccgcagcc agtcctccgg agccatccgg
     1201 aaccgttcct ccggctcacc ggagatattc tcgctgggaa gatacctgtc tccctccggc
     1261 gttcttacca cgaagccaca cgactccgct ggcgcacatc gccgggcgtg cgccagaatc
     1321 gctgattctg tctgtgtcat ggatttactg agaaagttta ttgatggaaa ggaaaccgcc
     1381 gaaattaccg gtattgttgc gcaactcaca cccgcgcata cacttgctgc atttgtcctt
     1441 ccggatatcc gtcgttggtt tgtcgaactc gtcagccact gccctgcccg tgtaaccaca
     1501 ctcatcagaa cggtaagtcc acatacaggt attcgccagc atgatacgcc ccgggaaaac
     1561 cgcgccatcc gtctccgttg gtgtggccag cacaaaagag gcactgaccg cgctcagttc
     1621 gctgcactgc tcgatgcgcc agcggctgat cacctcctgt tccggatcgg cgtcgctgtt
     1681 tccgttgacg aagttcaccg catccagaaa acgggcgtaa accttacgcc tgaccaccgt
     1741 tccgccgacc agactctgca ggtcttccgc catcccggtg accattccgt gcaggttaga
     1801 aaccgtcagt gtcggacggg cagcactgcc cttgccgttc agttcaaatc c
//
LOCUS       DAJTVJ010000308         1829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000308 DAJTVJ010000000
VERSION     DAJTVJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.314"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO448_004534"
     CDS             complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO448_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6339512.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     H"
     gene            complement(1400..1829)
                     /locus_tag="OO448_004535"
                     /pseudo
     CDS             complement(1400..1829)
                     /locus_tag="OO448_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          485 a          391 c          436 g          517 t
ORIGIN      
        1 atgggatgat aatgggttat cactaatatg tattgaacat tcattcctca gattaagaat
       61 gctttccggg atatgactta tctgatttct atcaaaaaaa tactctctga caacgggatt
      121 atttccctca gaaaatgctg gtagctcagt gaggaaatta cgggtcgctc tgagattttt
      181 aagagcctgt gggagcgatg gtaaggagac tatttcgtta tagctgatat tcatagtcaa
      241 taacgaatcg ggtaaagctg gcaggttttc cagctcattg tgttgtacct gaagtgactc
      301 cagcgcagga ggtagcacgg gcaattctga taatttattg ctggcggaat ataatttttt
      361 tagcgtaaca ggcagttcag gtaggttagt taattgatta tatgatacat tgagcagcgt
      421 tatctgagct ggtaagttgt caggtagcga ggacagattt aaacggtcca gtcgaagttc
      481 atcggaatta ttgataagac attctttaag tcgggagaca gcctcatcac gctcttcacc
      541 ggggagcgct tgtttttccc atttatccca tgctgaaaag taatcagcat atgtaccgga
      601 aatagtgtta taaaaagaat tttggggcaa tgaaaagtta ttatttatcg gtaacatgat
      661 gggcatccag ttaaagccgg tggacaaatg gctttgttaa aagaaataag taggagtttt
      721 tatgaagttc attccatgaa atgaacttca gcactttttt attataataa ggataaacaa
      781 aattggctgt aaaaaaacga tactgtttca gttaaccgtg aatggcctgt ttgcaccgta
      841 tgtgacagta atacgctgct tcatgataag ccctcaaaaa catacttatg atatgtatat
      901 gagtatatat taatggtaca aaccatcaat atccataaaa aatacaccat taattcgcta
      961 taagttttta atacaatttt ttctgctgcg atttttttat acgataatta gcatacttat
     1021 aacaccgtaa gtaaattaca tgaaagcagg cacagcgtga tccgattttt ctgcgtataa
     1081 cgattttccg gaagtgtaat acggttgcgc cccgggccgg gccccctttt ctgttgttgt
     1141 gtacgggccc acagagaatg gtaccggagg cgggctcaga agtactgagt cgggtgatcg
     1201 cgtcaggccg cgaagtactg acacgggtat tggtgactgg tgatgaagta ctgccccggg
     1261 tatgatttca ggctgattca ggttctgatt ctctgtcagt ccgcgtggtt gctggcctgg
     1321 ctgatttcct gttgtcaggc gttcaacaac cgactgctgg ttatagcccg gttcacgaac
     1381 acctgacggc aggtatcact cacccggtga aggtaaatcc ctcaagaggc agcaactctg
     1441 ccagtcgctc ctccggccat tccggcagac gcgtcaggac gtccgtcagc caggcatgtg
     1501 gctccagacc attacgtttt gctgtttaca gaaggctcat tatctgtgcc gcccggtttc
     1561 cggccatcag tgaaccggcg aacagccacg ctttccgtcc cataacaacc gggcgaatgg
     1621 cacgctcgca gatattgttg tccaggggca ccgctccgtc acccaggaag cagctcagtt
     1681 catcgcgccg gttcagaata tagttaatcg ccttatttaa tggcccgtcg ggcggacagc
     1741 acggctcctg ctcttcaagc caggcaaaca ggtcattcac tatcggccgg gagtaccgtt
     1801 atcgccactg ccgtattttt tccacaggg
//
LOCUS       DAJTVJ010000309         1796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000309 DAJTVJ010000000
VERSION     DAJTVJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.316"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(770..1306)
                     /locus_tag="OO448_004536"
     CDS             complement(770..1306)
                     /locus_tag="OO448_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6339513.1"
                     /translation="MYTQNVTTDKEREMRKKDLEEQDRYVSEKHTQKQKNQNFTQVYP
                     LGWKRLRELFRGNPGAAELYSMLAENIDGSCGAVVADQSHLASLLGVGRQTISRYVKW
                     LEEQGVLVKIPVAGKVCAYALNPHEVWKGYDNAKPYAAFLTKTLVNKDGDIQRRIMAM
                     FHGSNARQGMEGDTPEAE"
BASE COUNT          425 a          450 c          432 g          489 t
ORIGIN      
        1 aaaaaaaccc ctgaaacatc agggggtatg aagaggaatt attgtggagt aaccaatatt
       61 atttttatcg gctagttacg ggctggacaa acccgtaaca atgctgaatt tgccgtagtt
      121 gtgcctgaaa aacaatcctg atttcgggac aggatgtggg aatgtcggac cgcgcacgcg
      181 cactcagaca ttccccacaa ggtcagggct tcaccccgac accccgcacc agtgcgtacg
      241 tgctcaatgt aacttcaggt tctcttccgc ttcctcgccc actcgctcac tacgttcggt
      301 cgttggggct gcggcgagcg gtatcatctc tcgcatgttg tcggcgattc tgttgctgtt
      361 ctgttcgaaa atgcgttctg taagccatcc agagcaccag aacgcgattt tctggtgtcg
      421 ataatggttt ggtatgtatt ggcccttaaa acgcgctcag aagctcccag acgcacgaaa
      481 cggggtttaa atgcccgtca gggcatggga ggcgcgaaaa acgcttacag agggtttcag
      541 gaggtcaggc gcgccgatac ccctaaccag gggctttcag gtcgccagga accgcgtcag
      601 ccccctgcaa agctcatttg cacggcacag acagccactc ggttggtagg tggtcgtaga
      661 ccgtcggtgt tacagcgatg ggggtctggg gttctcccca gacggtttcc ggtagcgctc
      721 aaggcgctgc cggaaccgtg gcggctcgtt cgttctggag aacgggtatc tattcggctt
      781 caggtgtgtc gccttccata ccctgacggg cattgcttcc gtggaacata gccatgattc
      841 ggcgttggat gtcaccgtct ttgttgacca aagtcttggt caaaaatgcc gcatacggct
      901 tggcattgtc gtatcccttc cagacttcat gagggtttaa cgcataagcg catacctttc
      961 cagccacagg aattttgacc aatacacctt gttcttccaa ccacttaacg tatctagaaa
     1021 tagtttgtct tccaacgcca agtaacgatg ctaagtggct ttgatcagca acaacagcac
     1081 cacaagagcc atcgatgttc tccgcaagca tcgaataaag ctctgcggct cccgggtttc
     1141 ctctgaaaag ttctctgagg cgcttccacc ctagagggta tacttgagta aagttttgat
     1201 tcttttgctt ttgggtgtgc ttttcagaaa cgtaacgatc ttgttcttcg agatcttttt
     1261 tgcgcatttc tcgctcttta tccgtggtca cattctgggt atacataccc cctcctgaca
     1321 gttcattttt taatcaatct ctgtatgcga cacattgtcg cttgaatgta acatatacat
     1381 gtaaaaacca tgtgtcaatg tgtcgcatat gcgctacatg ttgggtaaat ctgtcgcaca
     1441 taagggatgc gacaacgttg tcgcaaaaaa gcctactttc aaaaaaccaa acctataaaa
     1501 atcaatcact tattattttc cgctcttatc tataaaaaat aggcctgcgg cgaagctcat
     1561 tatttgaccg atttctggga ggatagcgcc gcaggccgca catgtttctg agcgtcagcg
     1621 aagaaacata taagtgcgca tctctctaat ttcacgaatt tgagagatga aagcagagtc
     1681 ccgcactgag atcggctcta ggggctgctc tggaatctct ttacgacgat tcctccattt
     1741 cgaatttttt tatgtagcgt ttactacagt gctttcggcg cggggatttt taagtc
//
LOCUS       DAJTVJ010000310         1787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000310 DAJTVJ010000000
VERSION     DAJTVJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.317"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            159..1535
                     /gene="hpaX"
                     /locus_tag="OO448_004537"
     CDS             159..1535
                     /gene="hpaX"
                     /locus_tag="OO448_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6339514.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            1545..>1787
                     /locus_tag="OO448_004538"
     CDS             1545..>1787
                     /locus_tag="OO448_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6339515.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT          383 a          493 c          469 g          442 t
ORIGIN      
        1 cttatccggc ctacactcgc accaaaccgt aggccggata aggcgtttac gccgcatccg
       61 gcaaaaagcc gtaccaaatc gcggaccgga taaggcgtta cgccgcatcc gccataaacc
      121 ttgtcgtacc ctacaaaaat cccattagag gaagaaaaat gagcgacacc tcacctgcca
      181 taccggagag tatcgatccg gcgaatcagc ataaagcgct gactgccgga caacaggcgg
      241 ttattaagaa gctatttcgc cgcctgagcg tctttctgtt cgtgctgttt atcttctcgt
      301 tccttgatcg catcaacatc ggctttgccg gactcacgat gggacgcgac ctcggtctga
      361 gcgccaccat gtttggcctc gctaccaccc tgttctatgc cgcttatgtc atcttcggca
      421 ttcccagcaa cattatgctg agtattgtcg gtgcgcggcg ctggatcgcc accatcatgg
      481 tgctctgggg catcgcctct actgccacca tgtttgccac tggccccacc agcttgtacg
      541 tactgcgtat actggttggc attaccgaag ccggctttct gcctggtatt ctgctgtatt
      601 taaccttctg gtttccggcc tatttccgcg cccgtgccaa cgccttgttt atggtagcaa
      661 tgccggtaac gacagcgttg ggctcgatcg tttccggcta cattttgtcg ctggatggcg
      721 tcatggcatt aaaaggctgg cagtggctgt ttttgctgga aggcttcccg tcggtattac
      781 tcggcgtcat ggtgtggttc tggcttgatg actcaccgga caaagctaag tggctgacga
      841 aagaagacaa aaaatgcctg caagagatga tggataacga tcgtctgacg ctggttcagc
      901 cagagggagc catcagccac cacgccatgc aacaacgcag tatgtggcgg gagatcttca
      961 ctccggtggt gatgatgtat accctggcgt atttctgcct gaccaacaca cttagtgcga
     1021 tcagcatctg gacaccgcag atcctgcaaa gttttaatca gggcagcagt aatatcacca
     1081 tcggcatgct ggccgccgta ccgcagattt gtaccattct cgggatggtc tactggagcc
     1141 gtcactcaga tcgccgccag gaacgaaggc atcacaccgc ccttccttat ttgttcgctg
     1201 ccgcaggttg gttactggct tcggcaactg atcacaacat gatccagatg ctggggatca
     1261 ttatggcttc gaccggatca ttcagcgcaa tggcgatttt ctggacaaca ccggatcagt
     1321 ccatcagcct gcgggcacga gcgatcggta ttgcggtgat caacgccact ggcaacattg
     1381 gttcagcgtt aagtccgttt atgatcggct ggttgaaaga tttgaccggc agctttaaca
     1441 gtggattgtg gtttgttgcc gcgctgctgg tgattggtgc ggggattatc tgggcgattc
     1501 caatgcagtc ctcccgtccg cgagcgaccc tgtaaggaac gacgatgtgt gaccgtcaga
     1561 ttgccaatat tgatatcagc aaagagtacg atgaaagcct gggcacggac gatgtgcatt
     1621 atcagtcgtt cgcccgcatg gcggcatttt ttggccgcca tatgctgcca catcgccacg
     1681 aacagtactt tcagatgcat ttcctcaata gcggacagat tgagctacag cttgacgatc
     1741 atcgctactc ggtggaagcg cccctgtttg tcctgacgcc gccgtca
//
LOCUS       DAJTVJ010000311         1786 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000311 DAJTVJ010000000
VERSION     DAJTVJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.318"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1087
                     /locus_tag="OO448_004539"
     CDS             join(<1..183,183..1087)
                     /locus_tag="OO448_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6339516.1"
                     /translation="ERIGVAAAARELSLYESQLYAWRSKLQQQMTSSERESELAAENA
                     RLKRQLAEQAEELAILPKGRDILCEAPEMKYVFIEKHQAEFSIKAMCRVLRVARSGWY
                     TWCQRRTGISPRQQFRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAA
                     SLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGW
                     LYLAVVIDLWSRAVIGWSMSPRLTAQLACDALQMALWRRKRPRNVIVHSDRGSQYCSA
                     DYQALLKWHNLRGSMSAKGCCYDNACVESFFHSLKVECLHGEHFISREIMRATVFNYI
                     ECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            1427..1786
                     /locus_tag="OO448_004540"
                     /pseudo
     CDS             1427..1786
                     /locus_tag="OO448_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          436 a          444 c          478 g          428 t
ORIGIN      
        1 ctgaacgcat cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct
       61 atgcgtggcg cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg
      121 ccgctgaaaa tgcccgcctg aagcggcaac tggcggagca ggcagaggaa ctggctatcc
      181 tccaaaaggc cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      241 acatcaggct gagttcagca tcaaggcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      301 ctggtatacg tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca
      361 ctgcgacagc gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc
      421 acgcctgacg gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc
      481 aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta
      541 ccgcgcacat ggcctgcctg tgtcagaaaa tctgctggag caggattttt acgccagtgg
      601 tccgaaccag aagtgggcgg gagacatcac gtacttacgt acagatgaag gctggctgta
      661 tctggctgtg gtcattgacc tgtggtcacg tgccgttatt ggctggtcca tgtcaccacg
      721 cctgacggcg caactggcct gcgatgccct gcagatggcg ctgtggcggc gtaagagacc
      781 ccggaacgtt atcgttcact cggaccgagg aagtcagtac tgttcagcag attatcaggc
      841 gctgctgaag tggcataatc tgcgtggcag tatgagcgca aagggctgct gctacgataa
      901 tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa tgtctccacg gagaacactt
      961 tatcagccgg gaaataatgc gggcaacagt gtttaattat atcgaatgtg attacaatcg
     1021 ctggcgacgg cacagttggt gtggcggcct cagtccggaa caatttgaaa accagaacct
     1081 cgcttaggcc tgtgtccata ttacgtgggt aggatcactt acgccctgtg gttgaaacgt
     1141 gagcatattg ttttcagatg ctgttgtctt gcttcagagt gatctgtcct ccggatttac
     1201 gcaaagttat ccacaggcac tgtgcattta aaaaacttat cttttcttct tgattttaaa
     1261 aactcagatc gtcatatcat tatgataaac ataataaaaa actctaaaca tgagaaggtt
     1321 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
     1381 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
     1441 ccttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
     1501 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
     1561 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gctttcaacg gtgccggagt
     1621 tcgcgatact gcccgtacgc tgaaaatcgg catcaatacc gtcatacgga cattaaaaag
     1681 ctcccgcccc ggcggataac atctgcgcca gtcgctcacg ctgatgttgc ccttatctgt
     1741 gaacttgatg ggcagtggag cttcgttggc agcaaggcgc aacaac
//
LOCUS       DAJTVJ010000312         1700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000312 DAJTVJ010000000
VERSION     DAJTVJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.319"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(40..183)
                     /locus_tag="OO448_004541"
                     /pseudo
     CDS             complement(40..183)
                     /locus_tag="OO448_004541"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(187..1008)
                     /locus_tag="OO448_004542"
     CDS             complement(187..1008)
                     /locus_tag="OO448_004542"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6339517.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            997..1700
                     /locus_tag="OO448_004543"
                     /pseudo
     CDS             997..1700
                     /locus_tag="OO448_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1277..1393
                     /locus_tag="OO448_004543"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          439 a          377 c          398 g          486 t
ORIGIN      
        1 caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat gtttaagtcg
       61 atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat tgtagtattc
      121 aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat ttaggtagta
      181 acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta agcagttacc
      241 acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc aacgaggcat
      301 ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat tgagttgttc
      361 taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag taaggttata
      421 tgagactatt tctccattat acaggtccat gattggtgat aaatagagct tcctgccatc
      481 gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag cataaaattg
      541 tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac ggtacttctt
      601 gcgcctgata agtgagctga tacccagttc cttcattaac tttctgatgg ttttaccatt
      661 aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat aacgcccttt
      721 atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat ctggcgcatt
      781 aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa ccagtaattg
      841 tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt ttgttccaat
      901 cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc cccgggaatc
      961 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac tcgttttccc
     1021 ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa tatgactcac
     1081 aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag actctgcgtg
     1141 tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc accaccgctg
     1201 aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc agtaacgata
     1261 tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc tggaaaaaat
     1321 aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat gcagtgaact
     1381 gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc tgataaacgc
     1441 agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt atacgatgaa
     1501 aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga aggaatcagg
     1561 gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg tgttctccgg
     1621 ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga ccgcgtaaac
     1681 cgtcagttcg tggcagaacg
//
LOCUS       DAJTVJ010000313         1671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000313 DAJTVJ010000000
VERSION     DAJTVJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.320"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="OO448_004544"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="OO448_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCR6339518.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..>1671
                     /gene="istB"
                     /locus_tag="OO448_004545"
     CDS             1253..>1671
                     /gene="istB"
                     /locus_tag="OO448_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCR6339519.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATL"
BASE COUNT          411 a          420 c          475 g          365 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac t
//
LOCUS       DAJTVJ010000314         1631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000314 DAJTVJ010000000
VERSION     DAJTVJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.321"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            10..228
                     /locus_tag="OO448_004546"
     CDS             10..228
                     /locus_tag="OO448_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6339520.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            230..595
                     /locus_tag="OO448_004547"
     CDS             230..595
                     /locus_tag="OO448_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6339521.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            592..1257
                     /locus_tag="OO448_004548"
     CDS             592..1257
                     /locus_tag="OO448_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339522.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1257..1622
                     /locus_tag="OO448_004549"
     CDS             1257..1622
                     /locus_tag="OO448_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6339523.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          444 a          337 c          434 g          416 t
ORIGIN      
        1 cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag aacaacagaa
       61 ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct aatttttgtc
      121 tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt ggtatgggcg
      181 tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata tggcactgac
      241 gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt attgcggctg
      301 cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt ttgatggaaa
      361 tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc acccatgcaa
      421 tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg atggtcgtcg
      481 tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg ttgaaatgaa
      541 atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg catgaccact
      601 attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga tattgacgat
      661 atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact ggaagcagag
      721 cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt cggtgagtat
      781 gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg aatgcgacaa
      841 cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca agttgaccag
      901 gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg ttgggtacga
      961 ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc gccggcagtg
     1021 ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc agattcatcg
     1081 cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt tgccaacgtc
     1141 actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag taatacgttt
     1201 gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga taaataatga
     1261 ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa tgtattgggc
     1321 aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc atgcgtaagg
     1381 ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca gttcctgctg
     1441 aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt tcctgggata
     1501 aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa gacggcgttg
     1561 aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag gtgaatcaat
     1621 gacctggcct g
//
LOCUS       DAJTVJ010000315         1614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000315 DAJTVJ010000000
VERSION     DAJTVJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.322"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..699
                     /locus_tag="OO448_004550"
     CDS             <1..699
                     /locus_tag="OO448_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6339524.1"
                     /translation="DRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGN
                     PPVFSLTPNGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTV
                     GKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHERENNDIYSSCRL
                     IVRKNGAEIYNRTALDNTLIYSGVIDMPAGRGHMTLEFSVSSWLVNDWYPTASISDLL
                     VVVMKKATAGISIS"
     gene            767..1366
                     /locus_tag="OO448_004551"
     CDS             767..1366
                     /locus_tag="OO448_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6339525.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAGDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLAGSDDGRHSNTSLAW
                     GAGVQVNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1425..>1614
                     /locus_tag="OO448_004552"
     CDS             1425..>1614
                     /locus_tag="OO448_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6339526.1"
                     /translation="MNMAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASEN
                     PDEAGRYSMDVEYGQYSVI"
BASE COUNT          397 a          383 c          470 g          364 t
ORIGIN      
        1 gacaggattg cgatgattaa tcctgcgaat ggcaacacaa agccgatgtt tgttgggcag
       61 ggcgatcaga tattcatgaa cgacgtgttc ctgaaacgcc tgacggctcc caccattacc
      121 agcggcggta atcctccggt attttccctg acaccgaacg ggcggctgac ggcgaaaaat
      181 gccgatatca gcggtaacgt gaatgcgaac tccgggacgc tcaacaacgt cacgattaac
      241 gagaactgtc gggttctggg aaaactgtcc gccaaccaga ttgaaggcga tctcgttaaa
      301 acagtgggca aagctttccc ccgggactcc cgtgcaccgg aacggtggcc atcagggacc
      361 atcaccgtca gggtttatga cgatcagccg tttgaccggc agattgttat tccggcggtg
      421 gcattcagcg gcgctaaaca tgagagggag aataacgata tttattcgtc atgccgcctg
      481 atagtacgga aaaacggtgc tgaaatttat aaccgtaccg cgctggataa tacgctgatt
      541 tacagtggtg ttattgatat gccagctggt cgcggccaca tgacgctgga gttttcggtg
      601 tcatcatggc tggtaaatga ctggtatccc acagcaagta tcagcgattt gctggttgtg
      661 gtgatgaaga aagccaccgc aggcatcagt atcagctgaa ttttataacc catatacggg
      721 cgccagaaat ggcgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaagtttg
      781 tgcagccatt ttgtccgcag ccatctgtct ggccgtatcc ggtgcgcctg catgggcgtc
      841 tgagcagcag gccacactga gcgcagggta tcttcatgcc cgtacgaacg ctcccggcag
      901 cgataatctg aacgggatta acgtgaaata ccgttatgag tttacggaca cgctggggct
      961 gattacgtca ttcagttatg ccaacgctgg agatgagcaa aaaacgcatt acagcgatac
     1021 ccgctggcat gaagattccg ttcgtaaccg ctggttcagc gtaatggcgg ggccgtctgt
     1081 gcgcgtgaat gaatggttca gcgcgtatgc gatggcgggt gtggcttaca gccgtgtgtc
     1141 gactttctcc ggggattatc tccgcgtaac tgacaacaag gggaaaacgc acgacgtgct
     1201 ggccggaagt gatgacggtc gccacagcaa cacgtctctg gcgtggggag ctggcgtgca
     1261 ggttaacccg accgaatccg tggccattga tattgcttat gaaggctccg gcagtggtga
     1321 ctggcgcact gacggtttca tcgtgggtgt cggttataaa ttctgattag ccaggtaaca
     1381 cagtgttatg acagcccgcc ggttcaggcg ggcttttttg tggggtgaat atggcagtaa
     1441 agatttcagg tgtactgaaa gacggcacag gaaaaccggt acagaactgc acaatccagc
     1501 tgaaagcaaa acgtaacagc accacggtgg tggtgaacac gctggcctca gaaaatccgg
     1561 atgaagccgg gcgttacagc atggacgttg agtacgggca gtacagcgtt attc
//
LOCUS       DAJTVJ010000316         1590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000316 DAJTVJ010000000
VERSION     DAJTVJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.323"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1122..1457)
                     /locus_tag="OO448_004553"
     CDS             complement(1122..1457)
                     /locus_tag="OO448_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCR6339527.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
BASE COUNT          565 a          299 c          234 g          492 t
ORIGIN      
        1 atagtcacat aaaccaccga ttgagtgaca gttactgaaa caactatttc gacacaaaat
       61 ctcgtctatt caacatcatg caaatttgtc agtgactgaa tttagtgaca gaaccctctg
      121 gaattctgcc gtgtaaccac ttatatgcag acctgccccg ttgctctggg ttttcaggct
      181 gcgatgagtg atgagcgatg atttccatac agatgatata tcgactggaa ttgattgcct
      241 gcaattagat ttagttttat tgtgtggatc gttttagtta gtggggatga atttccgata
      301 aattaaccaa tggaaaggag atgatatgaa taaaaagata actggattat taataattag
      361 tctggcgtca attttcttaa ttgattgcac ccagtgccat taacatctat ctacacaaaa
      421 atagaggaaa acaattttga tatatcaaat aattacaagc agctccattc tttttattgt
      481 ttgggggatt acatctataa taattagaac aaagaattat ttttctaaga agaaaacaga
      541 ccttaatata agctttttta tagccttccc tatttctata acaatccttt tattttgccg
      601 aggtatatta taaatataac ataatacatc tatatggggt gtacatatcc ccacataaaa
      661 acacatactc tcattaacac gggaagaaac tatgttttcc aattctcact acatgtacat
      721 aatcgttctt gtactatggt ttagtattct caagtactta cagagaaaga atagcatatt
      781 acatccttta ttttcactaa tcctgacacc actaaccatt ttaatcatta gacttatgat
      841 agatttaaca tacaaccaat aaattataaa caaaataaaa atagggaaca ttatatgctt
      901 tataaaattt tcacattaaa cacaaagata aaaacaacta tcattacaat atgtgcatta
      961 tcatataatc ttacagcgca agcttccccg tcatacagac ctcgtgatcg agttaataag
     1021 actatgatag aactacaaac taacggagta gaacggcaac ttttagataa ttcttgtggc
     1081 ataagttctg gggtatttaa ataaaatata agacaggctg tctatcttac agacagcctt
     1141 tttataataa cagaaagaat atatatcata cttaacggaa gagataatat gaaaacagta
     1201 cccgtataaa cagcaagcaa tcccattttt ccaggtgtat cagcaaaaaa aacgctgttc
     1261 cagaaatcag gccgggtaaa tttcagagct gtatcttcaa tataccattt tgcaaccgga
     1321 tacaaaacca ttccgcagag agaaatacac cagaacagta atctgtattt aaaatcataa
     1381 tcccatgaca tatacagcat atatcctccc gtcacccatc cccaccacat attattaaaa
     1441 taatattttc tgttcatctg ccagttttcc tttcttttgc acttttgtca gtgtactgat
     1501 gcatgacaac ataaccacag catgataata ataatcaata acaataagct gtgtcacgtt
     1561 tacatcatgg aacggaggtg ctcctgtgac
//
LOCUS       DAJTVJ010000317         1510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000317 DAJTVJ010000000
VERSION     DAJTVJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.324"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(618..1289)
                     /gene="queE"
                     /locus_tag="OO448_004554"
     CDS             complement(618..1289)
                     /gene="queE"
                     /locus_tag="OO448_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6339528.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          480 a          311 c          281 g          438 t
ORIGIN      
        1 acaaactatt aacctaaaga aaaaattaac agacattaat tcaatattaa aaaaataata
       61 gattaaaatt tcttaacgat ttaagaatca tacgaataac actttgatta aatttaattt
      121 tttgtatcga taattgtgaa cctctctggc atggagaact attttgaaca tgaggtgtta
      181 cgtggatatt ttgcttatta caagtactgc taatataaaa acttgagaaa gagataacgg
      241 gttatatggt ggtttatccc cgctggcgcg gggaacactc taaacataac ctattattaa
      301 ttaatgattt tttaagccag tcacaatcta ccaactttac agtatcacac aaacaacaca
      361 tccattatgt taaagagtac ttaattcatt gattaaaaag gtaaatattt aaaataactc
      421 tatacaaatt aaaatctacc aaactttacc gcaataattt tcactccagc gaaaaattaa
      481 tgccacagaa tttgtagaga atgctaatga ttagccctgg atgggtaaaa tggaggagtt
      541 ttcagaggcg acaaacaata taaatgagta gaagtcttta caggttccac ccatttataa
      601 cgcttataaa tgtttaatca ggcaatattt agatatttat gtgtttgcat cgacaaacgc
      661 caattacgcg caatgcaggt ttcaatgcac aaacgtgtgg catcatcctt ttggctaatc
      721 ggctgcagtg caatgactcg cggtttatca tcggtcagcg ttgccaatag ttcatccagt
      781 gcttcaatat cgcgtacgcg ccccactgga tgcttgattt cgttggctcg ctccagtgcc
      841 tgtgacaaca cgtcatagcc gccgcgcatg ttcagctttg gcgatacggt aacccaggta
      901 ttcggtgtgc agcgtacctc atgagtacca ctggtttcga tctggcagct aaaaccgttc
      961 ttttcgagca gatcagtcag tggtagcaaa tcatgaatgc aaggctcacc acccgtaatc
     1021 accacatgcc gcgcggtgta tccctggcga ctaatgacag ccaacaagtc ttcactgctc
     1081 gccgcccccc acttatcact ctctttggtc ttcgccagaa tgctgaaaag ggagacttcc
     1141 cgatcctcaa gcttttccca ggtgtgtttg gtgtcgcacc aggcacagcc aaccgggcat
     1201 ccctgtaaac gaataaaaat ggcggggacg ccggtaaagt aaccctcacc ttgcagggtc
     1261 tggaacatct cgttaatcgg gtactgcata gcattctctg tgaagtggat aattgttaat
     1321 tattgcagat cctgccacaa caatcatgtc ttattaacat tctgttacag gcagatttaa
     1381 agaaaaatgc gaaacatatc gttaataatt aaagggagta acgtattatg tcagaagaaa
     1441 ataaagaaaa tggatttaat catgtcaaaa cattcaccaa aattatattt attttttctg
     1501 tattagtttt
//
LOCUS       DAJTVJ010000318         1418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000318 DAJTVJ010000000
VERSION     DAJTVJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.325"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..849)
                     /gene="hchA"
                     /locus_tag="OO448_004555"
     CDS             complement(<1..849)
                     /gene="hchA"
                     /locus_tag="OO448_004555"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6339529.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1105..1300
                     /locus_tag="OO448_004556"
                     /pseudo
     CDS             1105..1300
                     /locus_tag="OO448_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          365 a          284 c          366 g          403 t
ORIGIN      
        1 atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat tcgctgcgaa
       61 aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga tgtcgtcatt
      121 aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga gatgacccgg
      181 catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc aaatggaata
      241 accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc cgtggcaaag
      301 ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta cgtcctggct
      361 ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg catattcgct
      421 atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga acaacgattt
      481 atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc agtattcaaa
      541 cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag catggagatg
      601 atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt ttccgttatc
      661 ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac cgcgatacgg
      721 ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc taagcgaata
      781 ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt tacttgtttg
      841 aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata tttgcgactg
      901 agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa agcggtagtc
      961 ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt cacgccaaat
     1021 tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca ttatcaaagg
     1081 tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat gaatatttat
     1141 atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata tttctatatg
     1201 ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt ggtttgaaga
     1261 gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat cgatatgcat
     1321 tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct gaattcgatg
     1381 agggacgaac tcaggctagt gtttagtttt acagtaac
//
LOCUS       DAJTVJ010000319         1411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000319 DAJTVJ010000000
VERSION     DAJTVJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.326"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            563..>1411
                     /locus_tag="OO448_004557"
     CDS             563..>1411
                     /locus_tag="OO448_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6339530.1"
                     /translation="MIKSTNIQAIGSSIMHQINNIYSLTPFSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          499 a          279 c          232 g          401 t
ORIGIN      
        1 tacagatgga tatactgttt ttaataaaaa aaacagccat atacgaattg caaatacctg
       61 ctaccaaccg aacgaaaagg ttgaaattca cagctacaga aatacaatgg ttgacaaaga
      121 taaatgaagc cggaattgat aaaaaacaaa gccaacggta ttctgatttc tgaagttaat
      181 ttcagactcg tttaagctct attttcaaaa aaatatatca gggtttgtta ctaataactt
      241 ctgaaaacag aaaaaaatag agcagtaatt ctatttcaga gaggagtaac tccatctatc
      301 ctcgatacgt actgaacgtt atcgtgatat attcatcatg ctttcttcac cattattcga
      361 gtacagggag agacgcaatg accttaaata cctttttatg gctgaattac cattctcagg
      421 aataacagca gactccttcc catagacggc ttagggggaa agcaataccc ggagaaaggg
      481 tacaaagcaa actgttttaa aattaataag ctatattcta tttcactaga attcttatca
      541 ttgataagaa attttagtta taatgattaa atcaaccaat atacaggcaa tcggttctag
      601 tattatgcat caaataaaca atatatactc gttaactcca ttttctttac ctatggaact
      661 gactccatct tgtaatgaat tttatttaaa agcctggagt gaatgggaaa agaacggtac
      721 cccaggcgag caacgcaata tcgccttcaa taggctgaaa atatgtttac aaaatcaaga
      781 ggcagaatta aatttatctg agttagattt aaaaacatta ccagatttac cgcctcagat
      841 aacaacactg gaaataagaa aaaacctatt aacacatctc cctgatttac caccaatgct
      901 taaggtaata catgctcaat ttaatcaact ggaaagctta cctgccttac ccgagacgtt
      961 agaagagctt aatgcgggtg ataacaagat aaaagaatta ccatttcttc ctgaaaatct
     1021 aactcattta cgggttcata ataaccgatt gcatattctg ccactattgc caccggaact
     1081 aaaattactg gtagtttctg gaaacagatt agacagcatt cccccctttc cagataagct
     1141 tgaagggctg gctctggcta ataattttat agaacaacta ccggaattac cttttagtat
     1201 gaacagggct gtgctaatga ataataatct gacaacactt ccggaaagtg tcctgagatt
     1261 agctcagaat gccttcgtaa atgttgcagg taatccattg tctggccata ccatgcgtac
     1321 actacaacaa ataaccaccg gaccagatta ttctggtcct cgaatatttt tctctatggg
     1381 aaattctgcc acaatttccg ctccagaaca c
//
LOCUS       DAJTVJ010000320         1404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000320 DAJTVJ010000000
VERSION     DAJTVJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.327"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            211..>1404
                     /locus_tag="OO448_004558"
     CDS             211..>1404
                     /locus_tag="OO448_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6339531.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQGS"
BASE COUNT          294 a          437 c          377 g          296 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcaccag aaaaactcgc ttcgctgggt gagaagaagc tggcagccca gctctgcaaa
      841 cttgcgcctc gtctgggtaa acgccttgca gcagacatag ctcaggcact ggccgaacaa
      901 accgtcgtcg ttcccggcac gaatgccgct gccgtagtac tgccacgtct ggcactccag
      961 ctcatcacgc tgcgtaagca aagagacgag gtggcgcttg aggtagaaca gcgagttctt
     1021 gctcaccctc tttacccggt cctgaccagt atgcccggag tcggtgtcag gaccgcagcc
     1081 agactcctca ccgaggtcgc ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat
     1141 gctggccttg cgccggtaac tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg
     1201 cgacggggta ataaagctct caaacgggcg ttgttcctgt cggccttcgc cgcgctcagg
     1261 gatccgctct ccagggctta ctacacccgc aaaatgagtc agggaaaacg acacaatcag
     1321 gcgcttatcg ccctggcgag acgacgctgc gacgttctgt tcgccatgat gcgcgacggg
     1381 actttttata ccccgcaggg gtca
//
LOCUS       DAJTVJ010000321         1389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000321 DAJTVJ010000000
VERSION     DAJTVJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.328"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..745)
                     /gene="ipaH4.5"
                     /locus_tag="OO448_004559"
     CDS             complement(<1..745)
                     /gene="ipaH4.5"
                     /locus_tag="OO448_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6339532.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIC"
     gene            complement(1173..>1389)
                     /locus_tag="OO448_004560"
     CDS             complement(1173..>1389)
                     /locus_tag="OO448_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6339533.1"
                     /translation="AEEQKYEMLENEYSQRVADRLKASGLSGDADAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLSENGSRLHHS"
BASE COUNT          375 a          285 c          297 g          432 t
ORIGIN      
        1 tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta ttggaaacat
       61 ccagtgccaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt tcacctacct
      121 tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg ccggaaacaa
      181 agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta ccttcaatac
      241 gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta aaacgtgccg
      301 agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga ttgaatgaaa
      361 tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg ttaccattta
      421 catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca ttgttggaaa
      481 tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc agtaagctta
      541 aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc gcctggatgc
      601 gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg taatctctgg
      661 tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg gaacgaaaaa
      721 aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc ttggtctatt
      781 aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat cggcgttttc
      841 attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt atatcagtct
      901 acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt tatcccctat
      961 attttcaaac aatttttatc atttaactca ttactgtctc gatattccca tagcaaacga
     1021 tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg ccggtattga
     1081 gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt ccggagtcaa
     1141 tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtcg tgagccgttt
     1201 tcagacaatc gcagggccag tacctcgtca gtcaactgac ggtaaatctg ctgttcagtc
     1261 tcacgcatca cctgtgcacc ggcttccctc tccgcatccg catcaccgct cagacctgat
     1321 gctttcagcc ggtcagccac cctctgagag tactcattct ccagcatctc atacttctgc
     1381 tcttctgcc
//
LOCUS       DAJTVJ010000322         1309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000322 DAJTVJ010000000
VERSION     DAJTVJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.329"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..252)
                     /locus_tag="OO448_004561"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="OO448_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(367..1005)
                     /gene="ospI"
                     /locus_tag="OO448_004562"
     CDS             complement(367..1005)
                     /gene="ospI"
                     /locus_tag="OO448_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6339534.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1158..1309)
                     /locus_tag="OO448_004563"
                     /pseudo
     CDS             complement(1158..1309)
                     /locus_tag="OO448_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          380 a          328 c          211 g          390 t
ORIGIN      
        1 taactctttt atttgtttta tagcggcagc cagttctgag gcaggaacga catcctctcc
       61 ggcagcaaca gcagtgagac ttccttccag atattatttt ttccacttaa gcaacaggct
      121 gggctgaata ccatgcaaac gcgccacatg ggacaccgtc atgccaggct ccattgtctg
      181 ctgaataatg gcaatttttt cctgaggagt tttacgttta cgaagctcca gccccagcaa
      241 agttccagtc atctcaaaat tagaactagt gttagacata tatccaagcc tatctcttat
      301 tttaagatac atccactgtc tggcgtttca aggggccggc tcaaatctat gtacaggctc
      361 tcagcatcag caaagcctct tactcttcca actactactg ttagcatagt gatcaaattc
      421 ctgatcagat acatgaatta cctgaaccga actattttca tctacaaatg catctggcga
      481 tttttctaca ggatcataaa gattaaatcc ctttcctgtt tgaacatcca aaaacataat
      541 tttttcacca tcgttaaatc cagctatcca atgagtggcc tgttcaatag aaatcattac
      601 tacacttcct tttgaagtgt tttgtaaaat attttctgca atacgctcac gaaatttact
      661 ataagtttca ttaggatgtg acactatgcc ttcactatca atttgctcta aatgcctatg
      721 aaaaaatccg gtcatatact ctgatatttc attaggtacg ggagcctctc ttactccata
      781 ccctaacata gccatacaag catgaagcgc acaccctgaa cagttaccat ccccaccaga
      841 cggatttatc attttctgta aagtcgctgc caattcagaa tctttatcca tatcaccaag
      901 acttttagag tttaaagatt ccagcaattt tttaacattt acatttccct cctctgtatg
      961 tgcctcatat ccagcagttc cctgtaacga caccccatta atcatattgt atcctgacag
     1021 tatatatact cactatattt tcttcataac aaatatccct ctgatatgac aacaaaaaac
     1081 ggagccggat tcctgttttt gtgaactgca gctattcgag tctaaaaagt gatccactta
     1141 ccaccaacgg cctaatatat acagaacgga gtaaacaggg ctaacagttc ccgacaggtc
     1201 tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt gttgtacgca
     1261 taccagagcc agtgttgttg cagccttgct gccaacgaag cgccactgc
//
LOCUS       DAJTVJ010000323         1261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000323 DAJTVJ010000000
VERSION     DAJTVJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.330"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..193
                     /locus_tag="OO448_004564"
                     /pseudo
     CDS             1..193
                     /locus_tag="OO448_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            200..379
                     /locus_tag="OO448_004565"
                     /pseudo
     CDS             200..379
                     /locus_tag="OO448_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            429..638
                     /locus_tag="OO448_004566"
                     /pseudo
     CDS             429..638
                     /locus_tag="OO448_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(738..923)
                     /locus_tag="OO448_004567"
                     /pseudo
     CDS             complement(738..923)
                     /locus_tag="OO448_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          312 a          273 c          299 g          377 t
ORIGIN      
        1 cagtagtttt gtccgctcac accagtacaa accagtcgaa ataccggtaa cgacgattac
       61 tcttgtctct gatgccggtg gactacgaga ttttatttac tggcgtcctg atgcggtcag
      121 gactggtatg gaacctgttt atgtgatact taatgctctt ctggattccg ggcgatttac
      181 gcgtaaactt attctgttag ggctgtcagg ctcattttcc tatatcttcg gctcaattgt
      241 tgcgacactg ggaatgggac ttgttgtcga ttatctggga tggggggcca catttatcgt
      301 actgatactg tctgctgttt ttgccatcat tttcacactg atgagtcgtg aacgctcact
      361 ggagttcgaa aaagagtaat tttttcagga aaaaaataaa attactgact aaatatttat
      421 tttttgaatc gcagggcatt ccggataagg ttgatatcgt gtcacggctg aagacctatg
      481 gatacagtat ttctggtgtg gagacggatg acggatataa ggcgcttgtc cgcgcttttc
      541 agcttcattt ccggcagaaa aattatgacg gcataatgga tgctgaaacc gctgccatcc
      601 tgtatgcatt gctggagaag tatttcccgg gtaaataagg agtgagtcgt cagagctgcg
      661 atatatcccc ttcctccatg ggctgtctct tatacacaaa tccccacccg gggatttgtg
      721 atgtcagatg tcccctgtaa cactatgttt tcattgcgct ctatgaagct gagtgagcgt
      781 aacgactgga gctgcttctg cggtgctccg gtggcgaacg tgaagtcata ctcttcgaac
      841 attttcaccg ccgggaaggc taccattcgg gtatacatcg cctgtttacg ttgatgacgt
      901 gccagttttt cttcatgaag catgatgctt caagaagggc actgttgcaa agttatcgat
      961 gaggtagctg accatcactg ctgtcggaat cgatcttgcc cttctgtttc cagtcgcctc
     1021 agcaaactat ttttcagtta ccacctgtcc tgcggtaagc gtaaaccgac caccgtatgt
     1081 agccattaga caagaattgg taacttagac gcccatctga catagacgaa cacctaagta
     1141 tggaattaca ggactggcga aaagaacctc gtaaaaagta ttcgaatgaa ttcaaacttc
     1201 gggtgatgtc ttcaatcgac agaagtttct ggtaatctac tggtatttaa ggttttccat
     1261 g
//
LOCUS       DAJTVJ010000324         1215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000324 DAJTVJ010000000
VERSION     DAJTVJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.331"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..602)
                     /locus_tag="OO448_004568"
     CDS             complement(<1..602)
                     /locus_tag="OO448_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6339535.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVV"
     gene            complement(860..>1215)
                     /locus_tag="OO448_004569"
     CDS             complement(860..>1215)
                     /locus_tag="OO448_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6339536.1"
                     /translation="VAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHV
                     ENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAM
                     DRNYLASVLAGSGLS"
BASE COUNT          264 a          360 c          272 g          319 t
ORIGIN      
        1 accacgaccg tcagtacccg gtatgcgggc ggctccggcc agctgagtat ccaccacggc
       61 ccctgaaggc tgtttgtcgc caggtacaca tgcggactca ggcgaacatc ttccggcagc
      121 acctgccaga gcgcgcttag cggctgcgaa ctgtgctgcg ccgctactcc gccccgcgcc
      181 agccagagtg actcgctgcg gctgtagctt atctcgccgg gaaacagcgg ctcaaagtga
      241 atgctcctgc cgccgctgtc gttgagtatc aggccttcat cgcgtatctg taagcggata
      301 tcgaagggcg ctttccagcc gggaccaaac acccccaccg gcgcgggtgt tctggtccgg
      361 tagctgctgt aggcgcggaa aagaataaac ggcagcgggc agggcagcgc aaggtccgtc
      421 tcgcccggca gcaccttcgc acccagcacc gggttcaccg ggttagcata ggtaatccct
      481 cccggacaca ccgaacaggc cactcccgtc ggcgcaccta tcagcacacc cgctgaaccc
      541 tgcacaatat ccagtccctt gcgggtcatg tcgccctgcc gcgccgccgg ttttccgctc
      601 atggtttatt cctcttcatc tgaattgatt tacattaggt tggttatttt ctcataccca
      661 caactatatt ttctctctga atttattacg tgcaagacaa tcgcagcact ctaactaact
      721 tcgatgcaat ttgatgttca gaaaaatccc tcattagatt attatcatga aatacagcta
      781 atcatcttta gcttatatat ttctcaatgt attcttttga aaggatccgt gccctttcaa
      841 actcttcagc agggcaagat tacgaaagcc cgctccccgc aaggactgac gccagatagt
      901 ttctgtccat ggctgctttt cgcatcttac gtcttaaccc tgccttgaat accttatcat
      961 tcgtcaaaat attaatagca atgtgccgta tccctgaaaa taattctgct gcatttcctc
     1021 ttcttatttt gcagtcgtct tcattcatta ccacgtccag acgccagtgc agcttattct
     1081 ccacgtgcca gtggtttcgg attgctgtgg cgaacttttc tgcagttaaa tcagcagaac
     1141 tgatataata tctgaccgtc atttctggct ctttcttttg ttctgctatt attgaccgaa
     1201 aggagactgc cacgc
//
LOCUS       DAJTVJ010000325         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000325 DAJTVJ010000000
VERSION     DAJTVJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.332"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1008
                     /locus_tag="OO448_004570"
                     /pseudo
     CDS             1..1008
                     /locus_tag="OO448_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          326 a          232 c          368 g          264 t
ORIGIN      
        1 tcgaaacaga gaatggcgaa caggcgaagg cgcggcaccg gagcctggcg gaggtgttgc
       61 aggaggacac gggggtgacg cttccggcgg agctggcggt gatgctgggg aggctggagc
      121 gggaactgcg tgcgggtgcg gtgagtgcgg agagtgaggc atggcttgcg cagtgcgggc
      181 tgacggtgga gcagatggaa agccagatgg aggcggaata catcccggag cggaggctgc
      241 atctttacca ctgtgaccac cggggactgc cgcaggcgct catcagcccg gaaggggaaa
      301 cggcgtggcg cggggagtat gacgagtggg ggaaccagct taatgaggag aacccgcatc
      361 acctgtacca gccgtaccgt ttgccggggc agcagtatga tgaggagtcg ggactgtatt
      421 acaaccgtca ccggtactac gatccgttgc aggggcggta tatcacccag gatccgatag
      481 ggttgaaggg gggaataaat ctatacacgt atccactggc accaatacgt tacaccactg
      541 gggttagaac gagttataag tgtatatggg cctcccgctc cagaccgggc aggtgctgaa
      601 actcctcttg ttcttactga tatgactgga ggagtgacta tttattatga tcctgaaaca
      661 ggtgattcaa tgacttttga tagtagcaat agaattgatc gtcgttctca acgtggtgct
      721 ggtgatccat atacaggtga agtcgttggt tgtgagacaa atggatcagg aatatcggca
      781 gcatatggca caacgaagat ctatactaca gatactagag caagatggct acatggtggg
      841 ggatcatcgt tgcgcgatcc ttatgctcct cgacaaggct ggaaaccaac tatgggatgt
      901 acccgtgcac agaatgaaga tgtagatgag ttgtgtaaga aagtgacaag ttggatgtat
      961 tcgcatcctg gagagagaat tagatatgaa cgttttaaga cacgttaatg cgattaacat
     1021 gggagtgtgc attctaattt tggttattgg atgtgcaaat gctaaagttc ttaaacgatg
     1081 tatattaccc gctcctgatg gtggcgtaga aaaactatca aaacccaact tacatggagt
     1141 aattaaaaaa gttgatttga acacaaatga agcggaaatt aaaataaaag
//
LOCUS       DAJTVJ010000326         1148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000326 DAJTVJ010000000
VERSION     DAJTVJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.333"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..360)
                     /locus_tag="OO448_004571"
     CDS             complement(<1..360)
                     /locus_tag="OO448_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6339537.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSL"
     gene            complement(460..816)
                     /locus_tag="OO448_004572"
     CDS             complement(460..816)
                     /locus_tag="OO448_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6339538.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(952..1059)
                     /gene="rprA"
                     /locus_tag="OO448_004573"
     ncRNA           complement(952..1059)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO448_004573"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          359 a          235 c          261 g          293 t
ORIGIN      
        1 caaactggga taagtgcctg catcgagaaa actgtttgcc attcccgcca gaaagccaaa
       61 aacataagcg ataatgatgt tattggtctg caggatgcca aaaaagaagg ccatatagca
      121 gcacatcccg agcatgataa aagggcggcg accaaagcga tcggataata atcctgcaaa
      181 aagcaagaca cttaatcgac caatgcccag cgatgagata actatcgaga cacccgcggc
      241 attagtctgc caaagtgtct ccagcgaggc catattcagg ctcatcaaaa tgacgcccat
      301 accatgcacc aggtaattaa aatacaaccc aagtgcggta gggtaatagg gatttttcat
      361 aggttctact tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag tatccattca
      421 ttattgttgt aacaacgaca attcctttct gtggaaaaat taacaaagcc gctccagatc
      481 gtgtgcatat aattcagcgg ctaccgattg atagatcttc cagtcaataa aattgccatc
      541 agggtagact tgttgaaacg cggttaagtc ggggaaaaaa cgcatggtgt tgttgccgat
      601 gcggttattt attttctcta tgatggcatg aagatgtttt tttcgttgct gacgcatagc
      661 caacagttgc tcgactacac aatcaggaat ggcgcatttg ccattttccc actgacgcca
      721 gctttcactg tcaccggtct gggcaatcca ggtcgcgcat tcatcaatag tcatggcaaa
      781 aatatggcgg agtgcctgga gttcataagc gttcatcgtg taatggctgc atgaaataaa
      841 caataaaata taaataacat tatcagcctg ctgacggctt gaagagagtc acagtatctt
      901 gtgcaacatt attggaaaga gtgaggggcg aggtagcgaa gcggaaaaat gttaaaaaaa
      961 agcccatcgt gggagatggg caaagactac acacagcaat tcgttgtttc actcagggga
     1021 tttccatgct tataaatcaa tatgttgatt tataaccgtg agctaatagt aggcatacgg
     1081 actttttgcg tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa tttgcgacag
     1141 gtactacc
//
LOCUS       DAJTVJ010000327         1138 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000327 DAJTVJ010000000
VERSION     DAJTVJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.334"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            109..366
                     /locus_tag="OO448_004574"
     CDS             109..366
                     /locus_tag="OO448_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339539.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            366..740
                     /locus_tag="OO448_004575"
     CDS             366..740
                     /locus_tag="OO448_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6339540.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            737..>1138
                     /locus_tag="OO448_004576"
     CDS             737..>1138
                     /locus_tag="OO448_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6339541.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          324 a          249 c          315 g          250 t
ORIGIN      
        1 gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg gcaatccgcg
       61 cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt gaacaaccag
      121 acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct gcgtcatgct
      181 gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat ggtacccgcg
      241 cttcgggaac tgatgcaagc gaagcatcgc gcacagaaag cagtagatga gctggtggac
      301 tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca ggaggtgctg
      361 cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact tactggcgac
      421 gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg gatgtggcgc
      481 ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg ataaagatta
      541 ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa gcaccactgg
      601 atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa atcaatatcg
      661 ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc ataatgcatg
      721 aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc caaaatcccc
      781 ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca ctgatgtgat
      841 ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt tcctggggaa
      901 aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag attatgcact
      961 cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa aaccggtagt
     1021 gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag cgagtaaaaa
     1081 gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta caaacaag
//
LOCUS       DAJTVJ010000328         1117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000328 DAJTVJ010000000
VERSION     DAJTVJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.335"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            84..1115
                     /locus_tag="OO448_004577"
     CDS             84..1115
                     /locus_tag="OO448_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR6339542.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAJTVJ010000329         1114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000329 DAJTVJ010000000
VERSION     DAJTVJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.336"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..734)
                     /locus_tag="OO448_004578"
     CDS             complement(<1..734)
                     /locus_tag="OO448_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6339543.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSM"
BASE COUNT          326 a          190 c          241 g          357 t
ORIGIN      
        1 ctcatggaga agtaaatccg tgggccgtgg tagtccggtt gggcggtttg ttgtaacaaa
       61 gtttgcatta ttctgggaga tagttgattt tctctaagat tgacaacgct atcagaactg
      121 aggccaagta tgctttccgg aattgccgtg atccggttct gattaagcca gaatattctt
      181 ataatatgat catctctttc tggaaaatct ggtattactt ccaaagcatt tctggctgca
      241 tcaagtaatt ccaatgacat tggtaacctc ggaagtacag acaggtgatt gtcacttaca
      301 ttaatatatt ctagtgacgc aggtaattca ggaagtgcga acaaatggtt atcactcaca
      361 tttatatatt ccagcgacac tggtaattca ggaagtgcag ataattgatt actgcttgca
      421 ttcagctctt tcaatgccct tggtagctcg gggagcattg atagttggtt attgcttaca
      481 ttaatttcat caagattgtc aggcaatcgt ggtagagatc tgagacctaa acaagataag
      541 tccaacgatg tttcactgtt ctccagacat aattggagcc gggtaaaagc agtttccctg
      601 ttttctccgg aaatgctgtt tttagtccat tcaacccatt cggagagata attattgtga
      661 acattgtcga ttgatgtagt tctgtaaaaa gagacgtttc cagtggatag ggggggatta
      721 tttacaggaa gcataataac ctcgcagaag gatattctga taaaatgtga tactgggaaa
      781 taataagctg aaataaaatg tttcaggatt gaaatttatt gttttcattt taaggaagag
      841 atgttgattt aactgtaaaa aaacgatact attttcttag atggtaaatg tctcgcctgt
      901 gctatatttt gtttttcggt tagttaaata tcgtacgttc atatttgaac gttctgccgg
      961 aatgcattat caatagaggt aaagtcgcaa ccccaaatcg taaaggaaac cgtagcacgt
     1021 cttaagcaag aacgtgctgc ggttggatgc tattttttcc ctgaagcgga aaacattact
     1081 acagtacctt gaaccttggt tttaacattc tcga
//
LOCUS       DAJTVJ010000330         1109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000330 DAJTVJ010000000
VERSION     DAJTVJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.337"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            759..1055
                     /locus_tag="OO448_004579"
     CDS             759..1055
                     /locus_tag="OO448_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6339544.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          330 a          213 c          236 g          330 t
ORIGIN      
        1 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
       61 tacgttgaaa gtgtctcaaa aggaagtcct tttaagactg gttatgaagg agctatgagg
      121 ttgattaggg gttggtacaa gcccaactaa ccgaatcaac tatgaagatt ttaggagggt
      181 aactgtaacc atcactttct aactttcacg taggaaattg ttaagtggtt tgatagttac
      241 accatgtgac caagcatcta tatatcgtta tatagccctg tatatagcac tatatgtagt
      301 gtcgagagag gaacgactaa cacgatacag tttaccctga aaagagactt cacagattct
      361 ttatatattt caataagtta gggtaaaaag ggactatacc gagactgtat agaatctgtg
      421 tagaattgca tagatacttg gaaatctact attaaattgc atagggactc acctgatatt
      481 gtcctgtttt taacaggtct cctagaaaac cgaatagact tcattcaagg gtcattgaag
      541 tcataatcat ttcagtagaa aaaccaagtc tcgattttaa atgcaaatag caatcatatt
      601 caataaggca agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttact
      661 ttatgcatgg tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata
      721 acgtacttta caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc
      781 aagacaaagc tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt
      841 tacagttact gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca
      901 gtaacgatcc aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc
      961 tggaagcagc tcaggcctgg gccgatgctg acccgtacgt agcggcaggt gtctatgaac
     1021 acgtttcagt gaaaccattt aagaaagtgt tctgaataga gattcaatat ttaagggcac
     1081 ggtttttgca accgtgttct tgtttggcc
//
LOCUS       DAJTVJ010000331         1096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000331 DAJTVJ010000000
VERSION     DAJTVJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.338"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1074
                     /locus_tag="OO448_004580"
     CDS             join(<1..167,167..1074)
                     /locus_tag="OO448_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HCR6339545.1"
                     /translation="EGASRLSLPEGTLGQWVTAARKGLGTPGSRTVAELESEILQLRK
                     ALNEARLERDIFKKSNSVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQ
                     HEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRL
                     RCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIK
                     DVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQE
                     QSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQ
                     RRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          312 a          261 c          286 g          237 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      901 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      961 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
     1021 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
     1081 aacaaatggt agtgtc
//
LOCUS       DAJTVJ010000332         1089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000332 DAJTVJ010000000
VERSION     DAJTVJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.339"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..174
                     /locus_tag="OO448_004581"
     CDS             <1..174
                     /locus_tag="OO448_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339546.1"
                     /translation="IIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKR
                     RCQQQFTSQEVQN"
     gene            184..>1089
                     /locus_tag="OO448_004582"
     CDS             184..>1089
                     /locus_tag="OO448_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6339547.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHV"
BASE COUNT          271 a          256 c          285 g          277 t
ORIGIN      
        1 atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag cgcgattatc
       61 gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga acagctggct
      121 cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa ctaaggaacg
      181 gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt gattcacccg
      241 cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc tgccccccgt
      301 gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg ctttttctcc
      361 ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct gaataacggt
      421 cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa cacagaacgt
      481 tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga gttctggtca
      541 ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa taatggctac
      601 gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct tggcgttggc
      661 gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg ccagacggta
      721 gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa tatcccgttc
      781 tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt ctcttttgaa
      841 gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata tctcgaatac
      901 tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg ctttactggt
      961 cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc atttaccacc
     1021 aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg caatctgccg
     1081 ctgcatgtt
//
LOCUS       DAJTVJ010000333         1088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000333 DAJTVJ010000000
VERSION     DAJTVJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.340"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="OO448_004583"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="OO448_004583"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6339548.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAJTVJ010000334         1050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000334 DAJTVJ010000000
VERSION     DAJTVJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.341"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            83..>1050
                     /locus_tag="OO448_004584"
     CDS             83..>1050
                     /locus_tag="OO448_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6339549.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          274 a          239 c          255 g          282 t
ORIGIN      
        1 ctggcgattt attaatgatt acggcgcagt cagtgacccg ataaaaatga cgctgtaagc
       61 aagggataag gataggcgta ttatgatgac gaccagaata gtggttggcc tcacggcagg
      121 gacgtgtctg attttctcgc aaaacctgat ggccgaggtc agtgtattca atccggcgct
      181 tctggaaatc gaccatcaat ccggagtcga tattcgccag tttaatcggg caaacctgat
      241 gcccccaggt gtttatagcg ttgatatttt tatcaacggt aaaatgtttg aacgtcagga
      301 tgtgacattt gttcaggata atccagatgc tgatctgcac gcttgcttta ttgccattaa
      361 aaaaacactg tcctcctttg gcataaaagt tgatgcgctc aaatcgttca atgatgtgga
      421 tgagacggtt tgcctcgatc ctgctccacg tattgaaggc tcatcctggc agtttgacag
      481 tgataaattg cagctgaata tatccattcc ccaaatctac atggacgcga tggcttatga
      541 ttacatcagc cccacgcgtt gggatgaggg gattaacgcg ctcaccatca actacgattt
      601 ttctggttca catacactac gttcagatta tggttcacaa gagacagata ccagttatct
      661 caatctgcgc aatggactga atattggacc gtggcggcta cgtaattaca gtactttaaa
      721 caccagcgat ggccgtgcgg aatacaactc cattagtacc tggatacagc gcgatattgc
      781 cgcgttaaga agccagatta tgattggtga tacgtggacg gcgagcgata ttttcgacag
      841 tacgcaaatt cgcggcgcgc gtttgtatac tgataacgat atgctacccg ccagccagaa
      901 tggctttgct cctgtggttc gtgggattgc aaagtccaac gccaccgtca tcattcggca
      961 gaatggctac gtgatttatc agtctgccgt tccacaaggt gcttttgaga tcaccgatct
     1021 caacaccgcc agtacaggtg gcgatttgga
//
LOCUS       DAJTVJ010000335         1011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000335 DAJTVJ010000000
VERSION     DAJTVJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.342"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..888
                     /locus_tag="OO448_004585"
     CDS             <1..888
                     /locus_tag="OO448_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6339550.1"
                     /translation="FGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPG
                     TKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEK
                     IGNNQAITVTNNQIQNIGVNQIQTVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVG
                     VAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQT
                     AVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPV
                     PDAPVPKDLPPGMPDMRQF"
BASE COUNT          289 a          227 c          299 g          196 t
ORIGIN      
        1 ttcggtaacc ttgcgatacc gcgcgtgggt caggaggtca ttgtggactt cctcaacggc
       61 gatccggacc agccgattat tatggggcgt acctaccacc aggaaaaccg cacacccggt
      121 agcctgccgg ggacgaagac gcagatgacc attcgttcga aaacctataa gggcagcggg
      181 tttaatgaac tgaagtttga cgatgcgaca gggaaagaac aggtctacat ccacgtgcag
      241 aagaacatga acaccgaggt gctgaataac cgcaccacgg atgtgataaa caaccatgcc
      301 gaaaaaatag gtaacaacca ggcgatcacc gttaccaata accagatcca gaacattggc
      361 gttaatcaga tacagacggt tggtgtcaac ctggtggaaa cggtgggcag taaccagatt
      421 atcaaagtgg gatcaaacca ggttgaaaag gtggggatca ttcgtgcgct gacggtgggt
      481 gtggcttacc agacgacggt aggcggcatt atgaatacct cggtggcgtt gttgcagtcc
      541 tcacaggtag ggctgcataa atcactgatg gtgggaatgg gctacagcgt caatgtaggg
      601 aataacgtca ccttctcggt gggcaagacg atgaaggaaa acaccggaca aacagcagtt
      661 tattctgccg gtgagcatct tgaactctgc tgtggtaagg caaggctggt gctgacgaag
      721 gacggaagca tatttctcaa cggtacgcac attcatctgg aaggggagtc ggatgtgaac
      781 ggtgatgcgc cagtgattaa ctggaactgt ggtgccacac aacctgtacc ggatgcgcct
      841 gtgccgaaag atttaccccc cggaatgccg gatatgcggc aattttgagt caggtgcaaa
      901 aaattaaaac ggaactggtg cagtgaccag atgaaccata ttaaaagcag aaggaattga
      961 ttcatgggcg gcggaaaacc ggcggcgcgg cagggcgaca tgacccgcaa g
//
LOCUS       DAJTVJ010000336         1001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000336 DAJTVJ010000000
VERSION     DAJTVJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.343"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1001
                     /locus_tag="OO448_004586"
     CDS             <1..>1001
                     /locus_tag="OO448_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HCR6339551.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          221 c          253 g          234 t
ORIGIN      
        1 cagtggtaac ctgatcgaag gtctcactgt gtactttgcc ttaaaaagcg gctctgccac
       61 attaacgtca ttaacagcgg tgaccgatca aaacggaatc gcgacaacaa gcgtgaaagg
      121 agcgatgaca ggtagcgtca cggtaagcgc agtcacgacc gctggtggaa tgcaaacagt
      181 agatataacg ctggtggcag gcccagcaga cgcctcaaag tcagtcctta agaacaatcg
      241 gtcatcattg aaaggagact ttaccgatag tgctgagcta catcttgttc tgcacgatat
      301 atcaggcaat ccgatcaaag tttctgaagg gctggaattt gtacagtcag gtaccaacgt
      361 gccctatgtg caagttagtg caattgacta cagtaaaaat ttctcaggcg agtacaaagc
      421 cactgttaca ggaggcggag agggtatcac aacgctgatc cctgtattga atggtgttca
      481 tcaagctggt ctgagtacca caatacaatt cactcgcgca gaagacaaaa taatgagcgg
      541 tacagtatca gtcaatggta ctgacctacc gacaactaca ttcccttcgc aggggttcac
      601 cggggcgtat tatcagttga ataatgacaa ctttgcccca ggaaaaacgg cggctgatta
      661 tgagttttca agctctgcct cctgggtcga tgttgatgct accggtaaag tgacatttaa
      721 aaatgtcggc agcaattcgg aaaggattac ggcgacacca aaatcaggag gccctagcta
      781 tgtatacgaa atccgtgtga agagttggtg ggtgaacgcc ggcgaggctt tcatgatata
      841 cagccttgct gaaaattttt gcagcagcaa tggctacacg ctccccagag caaactattt
      901 aaaccacagt agttcccgag gcatcgggtc actgtacagt gaatggggag atatggggca
      961 ttacacgact gaagctggtt ttcaatcaaa tatgtattgg t
//
LOCUS       DAJTVJ010000337          999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000337 DAJTVJ010000000
VERSION     DAJTVJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.344"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..768)
                     /gene="ospC2"
                     /locus_tag="OO448_004587"
     CDS             complement(<1..768)
                     /gene="ospC2"
                     /locus_tag="OO448_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6339552.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEV"
BASE COUNT          311 a          145 c          174 g          369 t
ORIGIN      
        1 aacctcttta aagttatcta agaaaaatga ttggtgctca tggtaaaaaa caggtggaat
       61 agcattatca aagtggtcgg ttaatgtcat atatccatat ggagagtcat gatcaatgat
      121 atacgcatta gcaccaaaat ctacagtatg atgtgtttta tttagtggga gtgtttcttg
      181 atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg ttagctattt cttcaatatc
      241 ctcagagaat gtgtttttat atgtttgtat attctcagac ttaattttat caaaggactt
      301 aatattaaga gtattatttg acaggttagc gttactttga tgtctgaagt taaaatcaga
      361 attaataata atagagagca agtgacgttc atccgaagaa agaggatgag ttttttgtat
      421 tatttcagaa agatagtgtc tggacacctt atcgaggtgc tcaagtgacc gaaatgatga
      481 ccgtgctcca gatggcgctt caatagaaag atttaatgat ttgaaactgg tcccttgaga
      541 gaacatttta ctataacttt gcgctgctat agacttcctt aaaaagcttt tcacagtatc
      601 agcacagtga tctaaatgtt tttcacttat gccagaagag gatgagtttg ttacgcatga
      661 tatgtttttt tgtaatgctt ttgtatcttt gttcggattt atttttccat caacaagatc
      721 aatattgttc tgaacattaa tatgatttac tgcttcaggt attttcataa aacatccccc
      781 attatgtgcg tttttatctg cagttattta gaataatatt atctaactta atattttctt
      841 gtatgtatta taacgaacat attttaatat gttgtacgat tttcactctg aaatcccctg
      901 agtttaatca cacttccttt actgaaagga attattgcaa cagtattatt tgtttttgga
      961 tatatatctt gcgccttgtt atgtagttaa tgctcagaa
//
LOCUS       DAJTVJ010000338          873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000338 DAJTVJ010000000
VERSION     DAJTVJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.346"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            62..873
                     /locus_tag="OO448_004588"
                     /pseudo
     CDS             62..873
                     /locus_tag="OO448_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          228 a          225 c          237 g          183 t
ORIGIN      
        1 cactgcggaa gacgtgaaaa gcacggggaa aaacgccgtc ttatccggtc agcgtgcaca
       61 ggcagccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
      121 gaccggcact ctgaaaacgg ctaaccatct gtctgaaatc gcagcagcag gcgaaaaggc
      181 acaacagaag tcccgggata atctggggct gaaaagtgcg gccacgatgg aagcacagag
      241 cgacatttac gaccggacaa aaggccgtct ggcgataccc ggcgcattcg gctttgggtg
      301 tgcttttctg cctgaagatg ttatccgttt tgacactaag agtgatttcc tggcctgggt
      361 aaggaatgcg ctgccaggtg aatattccgt tgctggcccc tacgacatca tcatacccga
      421 cacacggttt gaaggggtgc tcagcatccg gtggactgat gcacgccctg agacaacaga
      481 accgaggtac agagccaaat cccttacttt ttacggcatt aacggcccca tttatcacac
      541 ccgctactgc tactggccca tatccagact gactggctgg gtgaaaataa atataaccac
      601 agaagatatt atttacagaa tcgtggcgag ctctgtctgc aacagatggg gagaccctga
      661 cattggcggg ctgattattg ctgcgtacca gggagaagct gacggtgata aagtcatcag
      721 acttgtcagg gggcagtcat acagaggctc acgactggga ccggtgggga tttcagtgcc
      781 cagtactccc accggaacgt atatagcatc cccacaattt ttcattacgg gatgttcaga
      841 gcattcatta ccggggtcat atagcgccct gtc
//
LOCUS       DAJTVJ010000339          869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000339 DAJTVJ010000000
VERSION     DAJTVJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.347"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..757
                     /gene="istB"
                     /locus_tag="OO448_004589"
     CDS             2..757
                     /gene="istB"
                     /locus_tag="OO448_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039272515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HCR6339553.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQMRELLNGHYIIHRKNL
                     LITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGLMENPTTADA
                     ILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          266 a          195 c          244 g          164 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacaga tgagggaact gctgaacggc cactacataa tccacaggaa
      301 aaacctactg ataaccggtc cgacaggatg cggtaagagc tggatagcca atgcgctggg
      361 cgaacaggca tgtcgacaga aatacagcgt ccggtactgt agaaccgggc gattactgga
      421 acagttggct cagggacgtg ttgatggcag ttggctgaaa tatctgaaac agttgcaaaa
      481 aatacaggtt ctaatcctgg acgatctggg gctggaacag ttaagtaacg cccagtgtaa
      541 tgaccttctg gagatcacag aggatcgtta cggacaaagc agcacgatag tggtaagcca
      601 gttcccggta gataaatggc atggtctgat ggagaaccca acaacagcag atgccatcct
      661 ggacagactg gtacataact cacacagagt ggtccttcag ggagagtccc tgaggaaaaa
      721 tcccccgaca gtggaaagca gcgaaaaaac gagttaaaaa gcggtagaaa aagttaagtt
      781 cccggttgat cgaatggatg cgaatcgctg atcgaacttc gatgccaatc cgtgatcgaa
      841 tagagcggaa tacgcactca ggctacctc
//
LOCUS       DAJTVJ010000340          867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000340 DAJTVJ010000000
VERSION     DAJTVJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.348"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..391
                     /locus_tag="OO448_004590"
     CDS             <1..391
                     /locus_tag="OO448_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6339554.1"
                     /translation="EIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLLIQHPSATYFV
                     KASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLRPTVQLIPMNS
                     AYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            391..>867
                     /gene="umuC"
                     /locus_tag="OO448_004591"
     CDS             391..>867
                     /gene="umuC"
                     /locus_tag="OO448_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6339555.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT          202 a          190 c          236 g          239 t
ORIGIN      
        1 cgaaattgtg acttttccgc tatttagcga tcttgttcag tgtggctttc cttcaccggc
       61 agcagattac gtcgaacagc gcatcgatct gaatcaactg ttgatccagc atcccagcgc
      121 gacttacttc gtcaaagcaa gtggtgattc tatgattgat ggtggaatta gtgacggtga
      181 tttactgatt gtcgatagcg ctattaccgc cagccatggt gatattgtca tcgctgctgt
      241 tgacggcgag tttacggtga aaaaattgca actacgcccg acggtacagc ttattcccat
      301 gaacagtgcg tactcgccca ttaccatcag tagcgaagat acgctggatg tctttggtgt
      361 ggtgatccac gtcgttaagg cgatgcgctg atgtttgctc tctgtgatgt aaacgcgttt
      421 tatgccagct gtgagacggt gtttcgccct gatttatggg gtaaaccggt ggttgtgcta
      481 tcgaataatg acggttgcgt tatcgcccga aacgctgagg caaaggcgct gggtgttaaa
      541 atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc gctgtggtgt ggtttgcttt
      601 agcagcaatt atgagcttta cgcagacatg agcaatcggg tgatgtcgac gctggaagaa
      661 ctatcgcccc gcgtcgagat ttacagtatt gatgaggcat tctgcgatct gacaggtgtg
      721 cgtaattgtc gcgatctgac tgattttggc agagaaattc gcgcaacggt gctacaacgt
      781 acccatctta ctgttggtat ggggatcgcc cagaccaaaa cgctggctaa gcttgccaat
      841 catgcggcaa aaaaatggca gcggcag
//
LOCUS       DAJTVJ010000341          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000341 DAJTVJ010000000
VERSION     DAJTVJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.349"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..>863
                     /gene="frlA"
                     /locus_tag="OO448_004592"
     CDS             2..>863
                     /gene="frlA"
                     /locus_tag="OO448_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6339556.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAIT"
BASE COUNT          164 a          243 c          229 g          227 t
ORIGIN      
        1 tatgggaagc caggaactcc aacgcaagct cggattttgg gccgttcttg caatcgccgt
       61 cgggacaacc gtcggctccg gtatttttgt atctgtgggt gaagtggcaa aagcagcggg
      121 cacgccgtgg cttacggtgc tcgcgtttgt cattggcggg ttaattgtga tcccgcaaat
      181 gtgcgtctat gcggaactat ccaccgctta tccggaaaat ggcgcagatt atgtttatct
      241 gaaaaatgcc ggaagccgac cgctggcttt cctctccggc tgggccagct tctgggccaa
      301 cgatgcgccg tcattgtcga ttatggcgct ggcgattgtc agcaatcttg gctttttaac
      361 gcctatcgat ccgttgctcg gtaaatttat cgccgccgga ttaattatcg cctttatgtt
      421 gctacacctg cgctccgttg aaggcggcgc agcgtttcag acgctaatta ccatcgccaa
      481 aattatcccg ttcactatcg tcattggcct tgggatcttc tggtttaaag cggagaattt
      541 tgccgcccct accaccactg cgattggcgc aacgggcagc tttatggcgc tgctggcggg
      601 gatctctgcc accagttggt cgtataccgg catggcctct atctgttata tgaccggcga
      661 aattaaaaac cccggaaaaa ccatgccacg agcgctgatt ggttcctgtc tgctggttct
      721 ggtgctctac accctgctgg cgctggtgat ttccggcctg atgcccttcg acaaactcgc
      781 caattctgaa acgccgattt ccgacgccct gacctggatc cccgcactcg gcagcaccgc
      841 tgggatcttt gttgccatca cgg
//
LOCUS       DAJTVJ010000342          846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000342 DAJTVJ010000000
VERSION     DAJTVJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.350"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(9..581)
                     /locus_tag="OO448_004593"
     CDS             complement(9..581)
                     /locus_tag="OO448_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR6339557.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          270 a          173 c          156 g          247 t
ORIGIN      
        1 ttgataaatt atttcttata ttcgacagta aattgtgaat cagcgttcgc cgtaccagcc
       61 gttacggtcg cattggttgc cacatagcgt gcaataaact tcatttcagc cttgccttca
      121 gcatcgatag cggtataagc agagtcgtta taaagtttaa cctgcgaatt atcggactgg
      181 ttaaaaatac gaataccaac gccggttgcc gcaatcgctt gtcctgtacc ggtaaaatca
      241 ccagggttgc ctgctgtaag cggcgtaccc actttcaggt cgccgtcgcc gccaggcgct
      301 tctgtaccat caaaacgaac ggctgcttga gtgtacgttg ccgggcaatt ttcaagttta
      361 atactgaact tctgtggcga cgccagacta cctacacctg aaaatgcatt tttactgact
      421 ttacccagtg ggacaatttg atcggctgta tcggaggtaa cttcacaagt agtgtcgaca
      481 atttcaccga cgaagtgaac aacaccatct tctgccagtg catgcccggc cagcattaga
      541 gatgccagag tgaatgcacc gcctataata ttacgcttca taaaaactcc attgatataa
      601 tatatccttt accatgaatc atcacttata ttaataagtg tatttttgat tcaaaggaat
      661 agtacaccaa agagacggct ttatctaaaa ataatattaa ttacgcaaaa ataaaatata
      721 aattcattgt ttaaatatta tttaaacagt aaaaaatcaa gtttgttatc agacattaat
      781 gataattcag aatatattta tatagaaaga tagataaatt tattaatgtc agttataaaa
      841 aagggc
//
LOCUS       DAJTVJ010000343          825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000343 DAJTVJ010000000
VERSION     DAJTVJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.351"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            9..425
                     /gene="tnpA"
                     /locus_tag="OO448_004594"
                     /pseudo
     CDS             9..425
                     /gene="tnpA"
                     /locus_tag="OO448_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          260 a          181 c          153 g          231 t
ORIGIN      
        1 tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc
       61 atgttcacct ggtctttgtc accaaatacc gacgtcagat ttttgaccat gacgcgacag
      121 aaaaactacg cacttacttt tcaaatgtat gtgctgattt tgaagctgaa ctggttgaaa
      181 tggatggcga accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat
      241 ccagtctggt aaacagcctc aaaggcgtat cgggtaggtg actgcgacga gatcgaccag
      301 atattgcagt caggtattac tacaaaggcg ttttgtggag tgttggctat tttgccagta
      361 gctgcggagg tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg
      421 gttaggtgga aaaccacgcc ttatatccct gccctgaagg acggggtttt acggcgcacc
      481 ggataagcct ggagattatc tacaccagca tggacatcat tgtactatgc cccgtacatg
      541 ccccatcaca tcctcaacat caaagtatta gagccttgca aaccattatg cgtctcagtt
      601 tgtctcatct atcttacgca aaaaaaaata gaactaatat caagcggcgc tatattgcgt
      661 attaaataaa accacctcaa ttacattaat aaatctaaac aaacatcatg caaataattt
      721 ccatggttat ttctaggcta tttaaattaa ataaataaat tatgagtcaa tcataaataa
      781 caacctttaa tataatttaa ctaccaaatt tatattcgtt cacaa
//
LOCUS       DAJTVJ010000344          815 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000344 DAJTVJ010000000
VERSION     DAJTVJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.352"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..202)
                     /locus_tag="OO448_004595"
     CDS             complement(<1..202)
                     /locus_tag="OO448_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6339558.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCN"
     gene            complement(571..>815)
                     /locus_tag="OO448_004596"
     CDS             complement(571..>815)
                     /locus_tag="OO448_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6339559.1"
                     /translation="SAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPV
                     KASSEAVQMLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          240 a          182 c          126 g          267 t
ORIGIN      
        1 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcatcg gtgaatcgtg
       61 gtaacagcgt gtcatgctca gttgcagcac catctagact gaaaataaat tcacggtgta
      121 agttcttaaa taacaaaaat ggagttctcc agtaatcaga acccccatga tgcttcccga
      181 ttaaaatatg ctcaattgac ataagaaata aaaattttta ttgcaatcac tcttgacaag
      241 gtttactctc ttttaagaga tgcttaacag gttttctaaa cggatatcct ttctgggata
      301 tctatctttt atcttaaaag tttttacaga tactttaaag atatctgtat atattctgta
      361 cagtaacttt tcagagtctg aaaattttta taaaaaatga ctagccctct ttgaatctca
      421 tcttccccga atcttcgaaa atatctgtct agttgattcg gtagaatcta cctcatgatg
      481 tatacgtgtt tagtacccgt cacaccagct atgtgacccc cttggcaaaa aagccaaata
      541 tcagctacat ccatgtaact attcagtaac ttatttagca agtctgtaat aagttgctct
      601 agagattcct agctcttcct gaatctgcaa cctcgtttta ccttgtgaaa taagcatctg
      661 tacagcctct gaagaagctt ttacaggtct tcccatatgt accccttcag ctttagcacg
      721 ttcaatacca gcctttctac gctctgctat caagcttctt tcaagctgtg ccaacgttga
      781 cataatgcct aacattgctt cacccatagc gcttg
//
LOCUS       DAJTVJ010000345          813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000345 DAJTVJ010000000
VERSION     DAJTVJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.353"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          235 a          168 c          187 g          223 t
ORIGIN      
        1 gtaaaagcaa ttctggtagt acggtgataa ctggtcgtat agcaaatggt cagcaaatac
       61 cgctgccaac aggattttct gccagtcagt gctcatggag tgtgagcaat gcagaaaacc
      121 ctcaaggttg gaagccgaat tactttgcag gatcagttgc aacttatgat gcaaatcgaa
      181 ttgttaaatg tggtttttat gatgaataca atttccataa agggacattt agagctgatt
      241 taaccggaaa atgtagttat gtcgtcgcgt gtcagaactg agattaaatt gctggcgctg
      301 ctggcctacg tgccaatccg gtacctggaa atcatcatca gcgtcgatat ggacgaatat
      361 aaaaacattt acgttatatc ccaaaaacac acaggtctta gggaggttta aactgtgtat
      421 taacacctat cgtattgacg ggcgtgaaat ggcagaaaca gaagtcgttc ctattgacat
      481 gcctgactct aacggcgaga tgacttggca agccaaaaac tacacacagt attcgtccta
      541 ctttatgaag attacttgct tgaaatagct tattggcaaa tggcatatac agttattgaa
      601 ccattctgat cttcggttgt atcgcacgca gccgaccatg agttattggc tgatgggaat
      661 gacctggtaa gccatatgta accagttcgt gaggtgcata ttccgccgcc acctgtaaca
      721 cgttttccag ttggacaagt agctgttgct ccaggaaagc gccaacctgt ggcctgtgct
      781 gtagcaattt gaagctcacc agatcctacc ttt
//
LOCUS       DAJTVJ010000346          784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000346 DAJTVJ010000000
VERSION     DAJTVJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.354"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>784
                     /locus_tag="OO448_004597"
     CDS             <1..>784
                     /locus_tag="OO448_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCR6339560.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVV"
BASE COUNT          280 a          112 c          133 g          259 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggta
//
LOCUS       DAJTVJ010000347          726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000347 DAJTVJ010000000
VERSION     DAJTVJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.357"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            35..726
                     /locus_tag="OO448_004598"
                     /pseudo
     CDS             35..726
                     /locus_tag="OO448_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAJTVJ010000348          718 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000348 DAJTVJ010000000
VERSION     DAJTVJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.358"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(77..>718)
                     /locus_tag="OO448_004599"
     CDS             complement(77..>718)
                     /locus_tag="OO448_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6339561.1"
                     /translation="EQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADY
                     LPERCTVFITIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRA
                     HNGGIIGPGAVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLL
                     VADHTKFAASAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          183 a          186 c          169 g          180 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctc
//
LOCUS       DAJTVJ010000349          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000349 DAJTVJ010000000
VERSION     DAJTVJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.359"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(36..133)
                     /locus_tag="OO448_004600"
                     /pseudo
     CDS             complement(36..133)
                     /locus_tag="OO448_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(320..>702)
                     /locus_tag="OO448_004601"
     CDS             complement(320..>702)
                     /locus_tag="OO448_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6339562.1"
                     /translation="QDEIGLRYNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRY
                     YGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTG
                     LKRGYRLAEQVLMHELLARMRWCG"
BASE COUNT          176 a          207 c          138 g          181 t
ORIGIN      
        1 taaaaaaacg atactttttc gggtggcgct tccaatcagt agaatatgtg tttttcaatg
       61 aaggagccga tgacttttcg tggatttcca cggaacgcga gaagcagatt gttttacgtg
      121 ccagccgctt gatcgatagg aattaaaacc ccaaaaagat taaaaaaaca ccacaaaacg
      181 gatgtttctt caacaccact tttgctccat atgaacggaa ccgacgatta aactggatgg
      241 ctctgattga ttcagggtat gaatggcggt tttttgctcc gtttccctca aaatggacgc
      301 aacttcccct ctgcggctct cagccgcacc accgcatccg ggccagcagc tcatgcatca
      361 ggacctgctc tgccagacgg tagccccgct tcagccccgt aaaacgcatc tgactcccgc
      421 acagcacgca cttcagcggg tcaaccttca gtaacctctg atacatccct ctccaggtga
      481 tttgcatcgc cgtttttctc actgtctccg ttatgatgta caccacttct tccagtaacc
      541 gccgtttcgc cggactcaaa aaaccgtagt acctcaccat acggaacccc ttatccgcca
      601 catgccagga gaacctttcc atgaactcat ctccactcat caacaggtat tcttcccgtt
      661 ttgttcggtg actgttgtaa cgcagaccga tttcatcctg ac
//
LOCUS       DAJTVJ010000350          665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000350 DAJTVJ010000000
VERSION     DAJTVJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.360"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            193..462
                     /locus_tag="OO448_004602"
     CDS             193..462
                     /locus_tag="OO448_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339563.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..665
                     /locus_tag="OO448_004603"
                     /pseudo
     CDS             459..665
                     /locus_tag="OO448_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          166 a          154 c          194 g          151 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattt
//
LOCUS       DAJTVJ010000351          611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000351 DAJTVJ010000000
VERSION     DAJTVJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.361"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..66
                     /locus_tag="OO448_004604"
     CDS             <1..66
                     /locus_tag="OO448_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR6339564.1"
                     /translation="LDKIRRQNRIKAELQAVLDEK"
     gene            196..408
                     /locus_tag="OO448_004605"
     CDS             196..408
                     /locus_tag="OO448_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6339565.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          180 a          126 c          172 g          133 t
ORIGIN      
        1 ctggataaaa tccgccgcca gaaccggata aaggcagaac ttcaggccgt gcttgatgag
       61 aaataaaaaa aagcctccat cagagaggag gcagggaaat aaggcttaaa aggaaataac
      121 ttccgcttcc gaaaggaagc cagtccactg aattatttat cacaataata ctgacaacta
      181 aagggggaca agaaaatgaa tggattcaga aacagttcac ggaacggcca ggtctggcgt
      241 taccagcgtg ccggcgggcg ggccgttatt ctggaagtca gtggacgctg gatggaagcg
      301 gcagaagcat ggcgacgggc tgcctgtgtt gcgcccagga cagactggca gcagtttgcc
      361 cgaaaaaggg ctgaacactg ccaccggcgc tgccggggaa gggtgtaacg aaaaaagcag
      421 ccttcattac tccaggagtc agaaggctga aaggagttta tgagatttgc ttcttaatat
      481 ccggtcgggc agtatgaccg gacagttctg agtagcacag agtcttttcc gcattaagtc
      541 gattttttat attttgtgat taatttcaca aaataaggtg ttattcaatg tgtgctgccc
      601 tattcagggg g
//
LOCUS       DAJTVJ010000352          600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000352 DAJTVJ010000000
VERSION     DAJTVJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.362"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            200..307
                     /locus_tag="OO448_004606"
     CDS             200..307
                     /locus_tag="OO448_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6339566.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT          159 a          145 c          166 g          130 t
ORIGIN      
        1 cgtagtatcc acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggcttaatc
       61 cctggtggca ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac
      121 aacagaacgg gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc
      181 attacaacaa ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct
      241 ggcagcaccg atcctggcgg ggattattac cgcagcgatt gtcggctggt ggcgtaaccg
      301 gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc
      361 gcaggggtaa cagcctgcgg ccatttttgt ctttatctcc tccacaagta aatattggca
      421 acccgagctg gaagcatcat ccagagcact catgaaacga ctgtaacagc tgttgaaaaa
      481 ccggaatgca ctcttcattg caaacatttc agggatgaag tgaagatgaa ttgagacgta
      541 gtatccacat caagccggcg ttatccggtg aggcgcaatg ttgcgggggc tttatccctg
//
LOCUS       DAJTVJ010000353          579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000353 DAJTVJ010000000
VERSION     DAJTVJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.363"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(70..249)
                     /locus_tag="OO448_004607"
     CDS             complement(70..249)
                     /locus_tag="OO448_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339567.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPRGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(249..>579)
                     /locus_tag="OO448_004608"
     CDS             complement(249..>579)
                     /locus_tag="OO448_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6339568.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLSPVVMLAKSVTA
                     QTHMAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLAS
                     IRAFDVE"
BASE COUNT          122 a          189 c          133 g          135 t
ORIGIN      
        1 cgtcagctga ctgacgaggt actggccctg cgattgtctg aaaacggctc acaactgcac
       61 cattcataat cacatcgcat aaaccacaga ccggactgac tccggaaaaa cagaggcccg
      121 cccccgggcc tccccggatt catccgtttc cctgttcagc ctgacagcac gcccccgcgg
      181 ccggatgaca gactccgctt cggtaagcaa agcggtcttc tgtgattccg ccagttgcgg
      241 cttattcatt actcaacgtc aaacgcccga attgaagcca aatcatccag accgctcagc
      301 tcctctttca tttcacgctg acggcgataa atctcatcgt tgcgatcgac ctgcgcctgc
      361 accattgctg ccgccagttc ttccagttcc ggcatcgaca gtttcacctg ctgattatcg
      421 gcatcgctcc acgccatatg tgtttgtgct gtgacagatt ttgccagcat gactaccggg
      481 gacaggcggc ccagtgagtt ggggccagca ttccagatac gaccgttcca ttcaaacgtg
      541 aacggcttcg cctcctgttc tgtgcgccat gcttcaatt
//
LOCUS       DAJTVJ010000354          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000354 DAJTVJ010000000
VERSION     DAJTVJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.364"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>557
                     /locus_tag="OO448_004609"
     CDS             <1..>557
                     /locus_tag="OO448_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6339569.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAJTVJ010000355          554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000355 DAJTVJ010000000
VERSION     DAJTVJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.365"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..245)
                     /locus_tag="OO448_004610"
     CDS             complement(<1..245)
                     /locus_tag="OO448_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339570.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEI"
BASE COUNT          197 a          106 c           85 g          166 t
ORIGIN      
        1 ctaatttcag cagctgttgc ggtacgcttt aaacgccacg ggttagttgc atcatttgct
       61 ttcgcttttc ccgggcaatt tgtagaaaaa ccccaccagg cattttcata aattttgcta
      121 tcgaaaataa cctgatattt attaccacct tgttggttat tccatgcttc cattgccaat
      181 gctggcagag cggaacacac cagccccata ccaatcatag atttagtaaa tatatttaat
      241 ttcattataa tcccttgtga cgtaaaaact gcaaaacaaa agtcctacgc aggaattatt
      301 tacgttttac gagaaccctt caatatgaat taaagtgagg cattaaaaat aagaaaaaag
      361 cctgacaaat gaagaatttt aaaaacagaa acattcatat ttaaaatatt aaattgaatt
      421 gatattttaa atatgaataa tttattcgtt ctgacagtac gaataagata tgcagtcaac
      481 aaatgcaaaa agggcgccga agcgcccttt tcaattcaaa actaattaac gtgtaattag
      541 cccagaactt tagc
//
LOCUS       DAJTVJ010000356          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000356 DAJTVJ010000000
VERSION     DAJTVJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.366"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            86..334
                     /locus_tag="OO448_004611"
     CDS             86..334
                     /locus_tag="OO448_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339571.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(331..>532)
                     /locus_tag="OO448_004612"
     CDS             complement(331..>532)
                     /locus_tag="OO448_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339572.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          135 a          118 c          126 g          153 t
ORIGIN      
        1 gacttctggt tcgctcaatg gttcttacac aaacttaggt tcacatagag gttcattctc
       61 agggcggaat tcagggggca gtacattgtt tatttatgca tctggaggta atggaggatc
      121 tgccggaggt gcatgtgcaa atacatcccg actgcaggga tatgttggtg ggacgctaat
      181 tagcgtgaat gccagcaata accctgcata tggaaaaaca gcctttatca gctttgctgt
      241 acctgcaggt acttcctatc agataacatc ctatccaaca gaaaatacat catgtggtgc
      301 cggggtattt tcagtatttg gatatcaaac ttaattgagc gttacacacg acgcaataca
      361 acctgtctta tgctcgtatt tgtaccattt gcgtgaacct gctgggctct caactacctg
      421 gcagtagtgg gcatcctctg cgttgcccat tctggcaatg gcacagaagt cgaattgccc
      481 cagataacct gtggtggaaa gctgagtaac tttgagtttt ccacctattg tc
//
LOCUS       DAJTVJ010000357          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000357 DAJTVJ010000000
VERSION     DAJTVJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.368"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..227
                     /locus_tag="OO448_004613"
     CDS             <1..227
                     /locus_tag="OO448_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6339573.1"
                     /translation="RRGADGKLGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAAL
                     KVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            227..>501
                     /locus_tag="OO448_004614"
     CDS             227..>501
                     /locus_tag="OO448_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6339574.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSA"
BASE COUNT          117 a          102 c          170 g          112 t
ORIGIN      
        1 tgcgtcgcgg tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg
       61 tgatgtttgc cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag
      121 gtccggctgc cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc
      181 aacagcaggc ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca
      241 ctggaaagtg cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct
      301 gggcgatggt tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag
      361 cgtgaagata cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca
      421 cggtggcgtc cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt
      481 ttgtcgtaaa tggtcggcaa g
//
LOCUS       DAJTVJ010000358          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000358 DAJTVJ010000000
VERSION     DAJTVJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.369"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>490
                     /locus_tag="OO448_004615"
     CDS             <1..>490
                     /locus_tag="OO448_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6339575.1"
                     /translation="APDGDIIRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFT
                     DCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGD
                     LTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYD
                     VLD"
BASE COUNT          120 a          145 c          141 g           84 t
ORIGIN      
        1 gcccctgacg gcgatatcat ccgctaccgt tatgataatc cacacagtga cttaccctgc
       61 gcaacggaag atgccaccgg cagccggaaa accatgacgt ggagccgtta cggtcagttg
      121 ctgagcttca ccgactgttc cggttatgta acccgttatg accatgaccg cttcgggcag
      181 atgacggcgg tgcaccgcga ggaagggctg agtcagtacc gcgcatacga cagccgtgga
      241 cagttaattg ccgtgaaaga cacgcagggc catgaaacgc ggtatgaata caacgccgcc
      301 ggtgacctga ccaccgtcat tgccccggac ggcagcagaa acgggacaca gtacgatgcg
      361 tggggaaaag ctatctgtac cacgcagggc ggtctgacgc gcagtatgga atacgatgct
      421 gccggacggg tcatccgcct gaccagtgaa aacggcagcc acaccacctt ccgttacgat
      481 gtactcgacc
//
LOCUS       DAJTVJ010000359          477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000359 DAJTVJ010000000
VERSION     DAJTVJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.370"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            88..195
                     /locus_tag="OO448_004616"
     CDS             88..195
                     /locus_tag="OO448_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6339576.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          122 a          114 c          142 g           99 t
ORIGIN      
        1 cacctgacaa caccacgggg gccattcttg catccaaaca atacaagaat agccgcgaaa
       61 cgttgtcatt acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg
      121 catgatttag cggctccggt cattgctggt attcttgcca gtatgatcgt gaactggctg
      181 aacaagcgga agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg
      241 gatcaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca
      301 agcggataat attctgcgga atgcgcagca cagagtgaag acgtgacgga ttccggaaca
      361 gaaaaacagt cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg caatgttgcg
      421 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgca
//
LOCUS       DAJTVJ010000360          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000360 DAJTVJ010000000
VERSION     DAJTVJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.372"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>476
                     /locus_tag="OO448_004617"
     rRNA            <1..>476
                     /locus_tag="OO448_004617"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAJTVJ010000361          469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000361 DAJTVJ010000000
VERSION     DAJTVJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.373"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            58..>469
                     /locus_tag="OO448_004618"
     CDS             58..>469
                     /locus_tag="OO448_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6339577.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQA"
BASE COUNT           93 a          128 c          144 g          104 t
ORIGIN      
        1 gtactgcccg gagtgtggca cggttcactg gccggacgga gtcgccccgc ccttctgatg
       61 cttccccgtt ttgccgatat ctttcagcag ggaaaccgct ggcttaactg gctggagaag
      121 cagccggaag gggcagtacg tccggtggtg attgagtcgg tgaccaaaat catggcgtgc
      181 gggaccacac tgatggggta tacacagtgg tgctgttcat caccggactg ttgccataca
      241 aaaaaggtct gcttccggtg taaaagccgc tcctgcccgc actgcggggt gaaggctggc
      301 gcacagtgga tacagtatct gctgagcctg gtccccgact gcccgtggca gcatattgtg
      361 ttcacacttc cctgccagta ctggtccctg gtgttccaca accggtggtt actgacagag
      421 atgagccgca ttgcagcgga tgtgatactg gaaatctgcc atcaggcag
//
LOCUS       DAJTVJ010000362          453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000362 DAJTVJ010000000
VERSION     DAJTVJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.374"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..453)
                     /locus_tag="OO448_004619"
                     /pseudo
     CDS             complement(1..453)
                     /locus_tag="OO448_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT           74 a          144 c          136 g           99 t
ORIGIN      
        1 gcacttcacg gcggttcagg ctgtcggtga tggtgatgcg gtccttctca tactgatacg
       61 tgtagcttaa gcctgccggg ttaagctgtt ctgtcacccg cccgtcgctg tcgtaacggt
      121 aacggatttc cggccggccc gtgtgacggt gcgccaccat ccggccccgg tatttatcat
      181 cgtaagtaaa gctgcgcacc tgtttgccac tacggtcata caccaccgcc agttcgccgc
      241 gtggcgtcca gccatagcgc accagcggcg cggcaggtaa attctccggg tattccgggt
      301 cgtgcgtcag ccacacggca gacagatgcc gttgtcccgg ccatattcgg tgtaacccgg
      361 cagggtatca ggaaaagcgg acggttccgt cccgccggaa atggcctgct gccgggcttc
      421 ttctgcccgc tgcgcctgcg tggtcagtac cag
//
LOCUS       DAJTVJ010000363          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000363 DAJTVJ010000000
VERSION     DAJTVJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.375"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>452)
                     /locus_tag="OO448_004620"
     CDS             complement(<1..>452)
                     /locus_tag="OO448_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6339578.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPL"
BASE COUNT           75 a          145 c          128 g          104 t
ORIGIN      
        1 caacggctgt ggccgccatg ttctgtctgc cgggattacc gcaaaatggt tctccagcgt
       61 ggtgcctgaa cccctgcgtc ccggtaccgc ctgcggctgt tcgccgtgaa gatcgctcgc
      121 caccacctgc cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg
      181 tcccggccat atctccggcg aacggcttgt tccgcgcgcc acttctgcgt tgtttcgcca
      241 gccgtccatc tgccagcggg caaagttctg cccgtgggca cccttgaaac gtccggggta
      301 gtcatacact tcatactgcg tgcgctggta atcctggtac tggccttcct gatcaaaacg
      361 ccctggccag ccgggacgtt taaaggtgta gtctttggtc actacggaag aaggacggat
      421 ttgtgcgctg tagcgaaact ggctgatgca ga
//
LOCUS       DAJTVJ010000364          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000364 DAJTVJ010000000
VERSION     DAJTVJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.378"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(279..>433)
                     /locus_tag="OO448_004621"
     CDS             complement(279..>433)
                     /locus_tag="OO448_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6339579.1"
                     /translation="INNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAIL
                     GKRFEI"
BASE COUNT          120 a           98 c           82 g          133 t
ORIGIN      
        1 cctcactccc gtaaagacgg gagtcaagtt cccgctccga cataactgcg tgcaatggat
       61 tttgaaggaa ccgtcagccc attccccgga ggcaaacatg ggctggcaaa agtgtagcgg
      121 tattaggcac agctatttag gaaagaggga ccatctccat ctcattaaat ggaccacgcc
      181 agacaattca ctgtcatgct gatgctgcac tgtggggaca gaatcactca tgatgacttc
      241 gataatcgac gacattatta tttggccgag cttttaggtc atatctcaaa tcgtttgcct
      301 aatattgctc cattttttaa taaaaaatca atcatttttg aatctttgct tttcattgca
      361 ttgtcaagca tagtgtcccc actatttgct ttttgaaatt ttttgtttac atcaaccaat
      421 ccattattga taa
//
LOCUS       DAJTVJ010000365          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000365 DAJTVJ010000000
VERSION     DAJTVJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.379"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..208
                     /locus_tag="OO448_004622"
     CDS             <1..208
                     /locus_tag="OO448_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339580.1"
                     /translation="VELNGITYQACRGDFVVRLDGSTCLQLWNKEGWVVRREGDPLEV
                     AQWLPACHDAGMEVRVQINESAAP"
     gene            complement(228..>424)
                     /locus_tag="OO448_004623"
     CDS             complement(228..>424)
                     /locus_tag="OO448_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339581.1"
                     /translation="VLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLL
                     MASFHWWRRKVNRLPPWDDV"
BASE COUNT          102 a          120 c          121 g           81 t
ORIGIN      
        1 ggtggagctg aacggcataa cgtatcaggc ctgccgtggg gattttgtgg tgcgtctcga
       61 cggcagcacc tgtctgcagt tatggaataa agaaggctgg gtggtacgcc gggagggcga
      121 tccgctggaa gtggcgcagt ggctgccggc ctgtcacgat gcaggaatgg aggttcgcgt
      181 acagataaat gaaagtgccg ctccgtaaga acacgcctgt gcacggttta cacatcatcc
      241 catggtggca gtcggttaac tttcctgcgc caccagtgga aagaggccat gagcagtccg
      301 gacaaaaaac cccccgtgat acttataata atggcctcac ctgccaccat tcctgacggc
      361 ccccagtaga taaaccacat tgcacatccc caggaaatgc cccataagcc ccccgtcagc
      421 accg
//
LOCUS       DAJTVJ010000366          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000366 DAJTVJ010000000
VERSION     DAJTVJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.380"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            43..303
                     /locus_tag="OO448_004624"
     CDS             43..303
                     /locus_tag="OO448_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339582.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          112 a          104 c          117 g           83 t
ORIGIN      
        1 tgatttttac ctgtcagaac attatcagat ggaacgtgca ggatggatca ggaaatgacg
       61 ttttcactca gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat
      121 ctggacagcc acggcgtaac ttatctctgc gagtcagcca aagccggagc cgtgctgatc
      181 ttctggcatg aactggccat aaatggctat acctcaatga atgccataaa gagacaggag
      241 atcattgacg ccgaccatca gcgcctcaga aagctcatct ggccggagga tgactggaaa
      301 tgactcagag ccgccgaccg tcgccactgc agcggagagt gctgattgtg ctggctgccc
      361 ttgatgaaaa acggccagga cccgtgctga cgcgggacat agaacgggtg ctggag
//
LOCUS       DAJTVJ010000367          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000367 DAJTVJ010000000
VERSION     DAJTVJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.382"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..130
                     /locus_tag="OO448_004625"
     tRNA            54..130
                     /locus_tag="OO448_004625"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OO448_004626"
     tRNA            173..248
                     /locus_tag="OO448_004626"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAJTVJ010000368          403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000368 DAJTVJ010000000
VERSION     DAJTVJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.383"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>403)
                     /gene="fimZ"
                     /locus_tag="OO448_004627"
     CDS             complement(<1..>403)
                     /gene="fimZ"
                     /locus_tag="OO448_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR6339583.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKL"
BASE COUNT          123 a           76 c           78 g          126 t
ORIGIN      
        1 ataacttatc ggcaatttct ttattagata atccgctaac cagataacgt aatatggtca
       61 cttcacgatt agatagcaca gtgaccgttg aactattcgt actacattta ttgcttttta
      121 tatagttgag cgtttcgcta ggaaaaaacg tgtatccgga gaggatcatc tgaacgggat
      181 gaaaaatatc attctgatca ttgcatttac tgacaaaacc gttagcacca gcttgtatcg
      241 ctctgccagc ataaaagcat tctgatttcg atgataaaaa taacactttc actgtgctct
      301 ggatttgttt gatccttttc aggaaggtaa aaccgtctgt tccgggcaag tctatatcca
      361 taatgattaa atcaacagga cgggttcgga gataatcgat ggt
//
LOCUS       DAJTVJ010000369          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000369 DAJTVJ010000000
VERSION     DAJTVJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.385"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(34..306)
                     /locus_tag="OO448_004628"
     CDS             complement(34..306)
                     /locus_tag="OO448_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339584.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           87 a           94 c          109 g          104 t
ORIGIN      
        1 gtgaacaggc gtttcagcgg tcggggctga aaatcaccac cacttcgggt catccgcctt
       61 catctccgct tctgtttcgt ataaatcaaa acgacggcag gtatggcaga acgtgacgta
      121 ttcgtacggt gaatccgggt acattttgtg gaaggtttcc cagaaacaga ccgtgcagga
      181 gggatgaccg gtgataaagt ctgcaaatac ggactgataa gggtgattat tggctctggc
      241 gacggctttc agaacctctt tcaccattct ggtgtggact ttctggtgct ccaggttgtg
      301 tgacatggga actcattctg gatggttact ctgaaagctc atattctgcc acaccccgat
      361 ttgcagccgc caggctgccg tggttcaggg tcat
//
LOCUS       DAJTVJ010000370          382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000370 DAJTVJ010000000
VERSION     DAJTVJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.390"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>382)
                     /locus_tag="OO448_004629"
     CDS             complement(<1..>382)
                     /locus_tag="OO448_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR6339585.1"
                     /translation="DGAGRRFHLVLTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFP
                     DTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQ
                     VRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT           63 a          130 c          124 g           65 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgcacctgca tcccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatcggca ccgtactctg taccggcggg
      241 cagcgtgtcg gggaaggaag gagctgagag agggcaggga ctgtcagggg aagataacga
      301 agcggtgtgc ggtttacggg cctcttccgc ccgctgcgcc tgtgtggtca gcaccaggtg
      361 aaaacggcgg cccgcgccgt cc
//
LOCUS       DAJTVJ010000371          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000371 DAJTVJ010000000
VERSION     DAJTVJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.391"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..356)
                     /locus_tag="OO448_004630"
     CDS             complement(<1..356)
                     /locus_tag="OO448_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6339586.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          101 a           95 c          112 g           73 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac a
//
LOCUS       DAJTVJ010000372          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000372 DAJTVJ010000000
VERSION     DAJTVJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.393"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="OO448_004631"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="OO448_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6339587.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
BASE COUNT           84 a          103 c           97 g           87 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccgcccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct t
//
LOCUS       DAJTVJ010000373          368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000373 DAJTVJ010000000
VERSION     DAJTVJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.394"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..224
                     /locus_tag="OO448_004632"
     CDS             <1..224
                     /locus_tag="OO448_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6339588.1"
                     /translation="RNLTRERTQVGLQAARARGRKGGRPKTLSKDKQALAVQLYNEKK
                     HTVAQICVLMGISRPTLYKYIESARLFKK"
     misc_feature    complement(275..>368)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          105 a           85 c           99 g           79 t
ORIGIN      
        1 aacgcaacct gacaagagag agaactcagg tcgggcttca agcagcacga gccagaggac
       61 gtaagggcgg caggccgaaa acactgagta aggataaaca ggcccttgct gttcaactct
      121 ataacgagaa aaaacataca gttgcgcaga tttgtgtgtt gatggggata tcgaggccaa
      181 cgctgtacaa atacatagag tcagccaggt tgttcaagaa atgaacttag cgtacgtttt
      241 cgtacggttg ggcttcaaat ccctaatacg ttgacggggc aatatgcagt tcgctgcata
      301 gcggtccgac cccgtactgc tcacgcagct tatccagcag tggcatcatt ttttccagag
      361 gcggtcga
//
LOCUS       DAJTVJ010000374          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000374 DAJTVJ010000000
VERSION     DAJTVJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.396"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>356)
                     /locus_tag="OO448_004633"
     CDS             complement(<1..>356)
                     /locus_tag="OO448_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6339589.1"
                     /translation="NGIIVGRDRLARLRKELRLHCKQKRKFRATTSSDHNLPVTPNLL
                     NQNFTPTAPNQVWVADITYVATREGWLYLAGVKDVYTCEIVGYAMGERMTKELTGKAL
                     FMALRSQHPPAGLIHHS"
BASE COUNT           74 a           97 c           94 g           91 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggtgc tggctcctga gcgccataaa cagggcttta
       61 cccgtcaact ctttcgtcat gcgttctccc atggcgtaac cgacaatttc gcacgtataa
      121 acatctttga cgccagcgag atacagccat ccttcccggg tggcaacata cgttatatct
      181 gcgacccaga cctgattagg tgctgtgggc gtaaagttct gattcagcag attcggcgta
      241 accggcagat tgtggtcaga gcttgtggtc gccctgaact tgcgtttctg cttacagtgc
      301 agcctcagct ccttacgaag acgtgccagt ctgtcacgac cgacgataat tccgtt
//
LOCUS       DAJTVJ010000375          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000375 DAJTVJ010000000
VERSION     DAJTVJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.399"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>331
                     /locus_tag="OO448_004634"
     CDS             <1..>331
                     /locus_tag="OO448_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6339590.1"
                     /translation="TWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMSMWRYR
                     ITRLLSRKYPDLVMPDALAAEGSSKREWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYF
                     GSYLKKPP"
BASE COUNT           78 a           86 c           96 g           71 t
ORIGIN      
        1 acacatgggg gcgtgaccag cagtggcatc cgcatatcca tttatcgaca actgccggtg
       61 gtgtgacgtc gggccacacc tggaaaaatc ttcattttta cgcccgtaag gtgatgagca
      121 tgtggcgcta ccggataaca cggttgctgt cacggaaata ccctgacctg gtgatgccgg
      181 atgcgctggc agcagaagga agcagtaaac gggaatggaa tcgcttcctg gacacgcatt
      241 accgccgtgg ctggaatgtc aacgtatccc gggtgatgga taatgccccc catgtggcgg
      301 tgtactttgg ctcttacctg aaaaagccac c
//
LOCUS       DAJTVJ010000376          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000376 DAJTVJ010000000
VERSION     DAJTVJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.401"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(54..242)
                     /locus_tag="OO448_004635"
     CDS             complement(54..242)
                     /locus_tag="OO448_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339591.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT           83 a           78 c           99 g           63 t
ORIGIN      
        1 gtgaacagac gtttcagcgg gcggggagta aaagcggaca acatgaggac ggattaaccc
       61 ccggtatcct gtagccgggt aagggctgct gtgtcgccac ggcgctccag gatggaggcc
      121 gcgaccatcg caggtggtgg gtccatgggt ttgagcatct tcatccagtc ccattcacgt
      181 tgtgagagct tctcagcacc aacagagagc agaacggtat ctatactttt gcgggtcaac
      241 ataagaggat gagtccttaa taaaacaggt cctcagagca tacatattct gcggagcgtg
      301 cggcaacaac aggacgaaca ggg
//
LOCUS       DAJTVJ010000377          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000377 DAJTVJ010000000
VERSION     DAJTVJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.402"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            71..146
                     /locus_tag="OO448_004636"
     tRNA            71..146
                     /locus_tag="OO448_004636"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
BASE COUNT           86 a           72 c           75 g           83 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcg
//
LOCUS       DAJTVJ010000378          308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000378 DAJTVJ010000000
VERSION     DAJTVJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.404"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>308
                     /locus_tag="OO448_004637"
     CDS             <1..>308
                     /locus_tag="OO448_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6339592.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAJTVJ010000379          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000379 DAJTVJ010000000
VERSION     DAJTVJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.405"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..278
                     /locus_tag="OO448_004638"
     CDS             <1..278
                     /locus_tag="OO448_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6339593.1"
                     /translation="SVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTR
                     FGKDMPGAMAEYGREYMKRKTKHGLRSNVTLTEDSEDWMPSCAIGHK"
BASE COUNT           90 a           56 c           85 g           73 t
ORIGIN      
        1 cgtcggtaat gtgtgatggt ctgttagtgg gtaatgccga aattattccg ttcagtccgc
       61 gcaggtatct ttatcacgcc tatctggcat atatgagggc acacggattc ggtaaacctg
      121 taacactgac gcgcttcggt aaagatatgc cgggggcaat ggcggaatat ggcagggagt
      181 atatgaaacg gaaaacgaag cacggtttgc gttcaaatgt gaccctgaca gaggattcag
      241 aagactggat gccatcatgt gcaataggtc acaaatgacg atggaaaaaa ttaaacttat
      301 ggaa
//
LOCUS       DAJTVJ010000380          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000380 DAJTVJ010000000
VERSION     DAJTVJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.406"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>304)
                     /locus_tag="OO448_004639"
     CDS             complement(<1..>304)
                     /locus_tag="OO448_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6339594.1"
                     /translation="RFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMG
                     FSPRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLH"
BASE COUNT           71 a           86 c           84 g           63 t
ORIGIN      
        1 cgtgcagcca gcgcagaccg tggcggttgg ctgtttcaac atcgacagtg atccccatcg
       61 ggcgcaggcg agtcattagt gggatgtaaa aactgttacg ggtgtactgc accatccgtt
      121 ccaccttacc tttagtctgt gccctgaagg ggcgacacag tcggggagag aagcccatct
      181 ccttgccgaa ctgccacagc gaaggatgga accggtgctg accggtctga tatgcgtcac
      241 gttgcagaac cacagttttc atattgtcat acaacacttc gcgcggcaca ccaccaaaga
      301 agcg
//
LOCUS       DAJTVJ010000381          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000381 DAJTVJ010000000
VERSION     DAJTVJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.denovo.407"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>304)
                     /locus_tag="OO448_004640"
     CDS             complement(<1..>304)
                     /locus_tag="OO448_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339595.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKR"
BASE COUNT           69 a           84 c           79 g           72 t
ORIGIN      
        1 tacgcttcag tacagcatgc catggtcccc agagggagaa ccagtccgta aattcattct
       61 cttcacggct tctgaccatg gcttcggcag tgcggaggtc atttgctgtc actcccgaca
      121 cgccatagaa acgcatttcc ttcacggcag tggagagctg aagtttctct gcgagcatgg
      181 tctggaaggc caggtagact tctatctcat ccacaaaatg gagagttctg actttatccc
      241 gggcaatgtc ctccagaatt tcgaggcgga acatttccct gcccagggag agcagagcgc
      301 cggt
//
LOCUS       DAJTVJ010000382         6956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.guided.184, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000382 DAJTVJ010000000
VERSION     DAJTVJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.guided.184"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1453)
                     /gene="pepD"
                     /locus_tag="OO448_004641"
     CDS             complement(<1..1453)
                     /gene="pepD"
                     /locus_tag="OO448_004641"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6339596.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPA"
     gene            1714..2172
                     /gene="gpt"
                     /locus_tag="OO448_004642"
     CDS             1714..2172
                     /gene="gpt"
                     /locus_tag="OO448_004642"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6339597.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2264..3508
                     /gene="frsA"
                     /locus_tag="OO448_004643"
     CDS             2264..3508
                     /gene="frsA"
                     /locus_tag="OO448_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6339598.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3566..3967
                     /gene="crl"
                     /locus_tag="OO448_004644"
     CDS             3566..3967
                     /gene="crl"
                     /locus_tag="OO448_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6339599.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4006..5055)
                     /gene="phoE"
                     /locus_tag="OO448_004645"
     CDS             complement(4006..5055)
                     /gene="phoE"
                     /locus_tag="OO448_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6339600.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            5343..6446
                     /gene="proB"
                     /locus_tag="OO448_004646"
     CDS             5343..6446
                     /gene="proB"
                     /locus_tag="OO448_004646"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6339601.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            6458..>6956
                     /locus_tag="OO448_004647"
     CDS             6458..>6956
                     /locus_tag="OO448_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6339602.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSC"
BASE COUNT         1673 a         1746 c         1792 g         1745 t
ORIGIN      
        1 tcgccggaat ttctttcagc agttcagtca gcagtgtcca gtaatgacct acgctttcga
       61 tgtgaacttg ctcatccgga gagtgtggac cggtgatagt tggcccgata gaaaccatgt
      121 ccatttccgg atacggtttt ttgaacagac cacattccag gcccgcgtgg ataatctgga
      181 tgttcggcgt tttgttgaac agacgctgat aggtttcacg taccagatgc atcaccggag
      241 aattagcgtc cggctgccag ccaggatatg cgcctttcgc ttcggttttc gcgccagcca
      301 gtttacccag cgaatccagc atgctcacca cgtagtcttt accgctgtcg atcagagaac
      361 ggatcagaca gtgaatttct acgttattat cagtcatggt caccacaccg acgttcaggg
      421 aggtttcgac cacacctttt gccacgtctg agttgcggat cacaccgttc ggggtggcgt
      481 tcagcagacg aataaaggta tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta
      541 cagagtccag caacaaggcc agatttttct ctttctctgc cagctcgttt ttcaggatct
      601 cctgataggt attcaccaga gatttcagga cgtcgacttt atcagctgcg acagcaatgg
      661 tcgcaaaggc ttcacgcggg atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc
      721 gcagatccag ttcttctgca tgacccgcta ggaagcgcac cagcagtttg ttggcattac
      781 ccaggccaac gtggatttcc ccgccggagt gacccccttt cagacctttt aaggttaact
      841 tgaaggtttc aaaaccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt
      901 cgataccccc cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca
      961 gaatatcagc ctgcaaccag ttgctctgta agccgaacgc accgtccata ccggcttctt
     1021 cggtcatggt cagcagcact tccagcgggc cgtgaaccac gttttcgtca gccagaaccg
     1081 ccagcgcaga cgccatacca atgccgttat ctgcacccag cgtggtgccg cgcgctttaa
     1141 cccattcgcc atcaatataa ggctggatag gatctttcgt gaagtcatgc acggtgtcgt
     1201 tatttttctg cggcaccata tcgaggtggg cctgtaagac gaccgattta cgattttcca
     1261 tacctgcggt agcaggttta cgaatcagga tattacctac ctgatcgcgt tcgacatgga
     1321 aacctttctc ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg
     1381 ggtgaggaat agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag
     1441 acagttcaga cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca
     1501 gggttagcag aaaatgttgt caacacaaga caggcttgcg agatatgttt gagaatacca
     1561 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
     1621 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
     1681 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
     1741 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
     1801 aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa
     1861 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
     1921 gagcttaaag tgctaaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
     1981 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
     2041 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
     2101 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
     2161 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
     2221 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
     2281 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
     2341 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
     2401 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgtgaaat
     2461 cctcgacgtc caggcgcgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
     2521 cgatacggtg attggctacc gtggtggcaa ctggatttat gagtgggcca tccaggcgat
     2581 ggtgtggcaa caaaaagcct gtgcggaaga agatccgcaa ctcagtggtc gtcactggct
     2641 gcatgcggct acgttgtaca acattgctgc ctatcctcat ctgaaaggag atgacctggc
     2701 cgagcaagcg caggctttgt caaaccgtgc ctatgaagaa gccgcccagc gtctgccggg
     2761 cacgatgcgg cagatggagt ttaccgtacc cggcggtgcg cccatcaccg gctttttgca
     2821 tatgccgaaa ggcgatggcc cgttcccgac agtattaatg tgtggtggtc tggatgcgat
     2881 gcagacggac tattacagcc tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct
     2941 gactattgat atgccgtcgg tgggcttttc atcaaaatgg aagctcaccc aggactccag
     3001 cctgttgcat cagcacgttt taaaggctct gcctaacgta ccgtgggtgg atcacactcg
     3061 cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc gtgcgtctgg catatcttga
     3121 atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta gttcataccc tgttgagtga
     3181 ttttaagtgc cagcaacagg tgccggaaat gtatcttgac gttctggcga gtcgtttggg
     3241 gatgcatgat gcttccgacg aagcgttacg cgtggagctg aatcgctatt cattaaaagt
     3301 gcaaggattg ctggggcgtc gctgcccaac gccaatgtta tcaggctact ggaagaacga
     3361 tccgttcagc ccggaagagg actcacgctt aatcacctca tcatctgctg acggtaaatt
     3421 attagagatc ccatttaacc cggtgtatcg gaattttgac aaaggtcttc aggaaatcac
     3481 cggctggatc gaaaaacgct tgtgttaaaa atttgctaaa atttgccaat ttggtaaaac
     3541 agttgcatca caacaggaga ttgcaatgac gttaccgagt ggacacccga agagcagatt
     3601 gatcaaaaaa tttaccgcac taggcccgta tattcgtgaa ggtaagtgcg aagataatcg
     3661 attctttttc gattgtctgg ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga
     3721 gttctggggc tggtggatgg agcttgaagc gcaggaatcc cgttttacct acagttacca
     3781 gtttggtctg ttcgataaag caggcgactg gaagagtgtt ccggtaaaag acactgaagt
     3841 ggttgaacga ctggagcaca ccctacgtga gtttcacgag aagctgcgtg aactgctgac
     3901 gacgctgaat ctgaagctgg aaccggcgga tgattttcgt gatgaaccgg tgaagttaac
     3961 ggcgtgagtg aaatgtgccg gatgtatcac atccggcaat atccattaaa actgataggt
     4021 catgccaacc gcgacaatat catcattatt aatattcaat ttgttatcgc tatccagttg
     4081 gttgatttta taatcaacaa acgctgacat atttttgttg aaataatacg tagcaccgac
     4141 gtcgatataa ttgaccagat cttcatcacc gataccttca atatctttcc ctttcgataa
     4201 gacataaccc agcgatggac gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa
     4261 gttctgtgtc ttattggcaa agccgccagt tattggcgtc attttgcgtg tttcagaata
     4321 gaaagttgcc agataaatat tattggcatc gtatttcaga cctgttgccc atgcttctgc
     4381 acgcttgcct gtgccacggc tttgcaggtt ctgctcgttg gtgcgatctg agttggtata
     4441 ggccccacta atggcgaaat cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc
     4501 atcgccgttt tgctttttaa cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa
     4561 gttcaggcca tcgataacgc cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc
     4621 gcgtttggtc ataaagttgt cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat
     4681 atcggtccag gcttccacgt catacaacgc gcccaggtta cgaccataat cgaaagaacc
     4741 caaatcttta tatttcaacc cggcaaaagc gagacgcgtt ttctgctgtg cagtatcact
     4801 ctccgcttta tttccggcaa actccgcttc ccaccgacca taaccagtca gttgatcgtt
     4861 aatttgtgtt tcacctttaa aaccaaaacg gatataactc tggtcgccat ctttactggc
     4921 gttatcactc atataatgca taactttgcc atagacatcc agtttattac cgtctttatt
     4981 atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca ctaatgccag
     5041 agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc agcgggagag
     5101 ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt acagattaat
     5161 aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct
     5221 aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga
     5281 cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa
     5341 tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat
     5401 cgcgccgtct gaaccgtgcc catattgttg aacttgttcg ccagtgcgcg cagttacatg
     5461 ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc
     5521 tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggca gcggtagggc
     5581 agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc
     5641 aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca
     5701 ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac gatgctgtcg
     5761 ctacggcaga gattaaagtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg
     5821 cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgacccgc
     5881 gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg
     5941 cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtacc aaattgcagg
     6001 ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg
     6061 gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga
     6121 ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg
     6181 tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca
     6241 ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag
     6301 gccgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcattacgc cgtattgccg
     6361 gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc
     6421 accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc
     6481 tgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct
     6541 ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca
     6601 ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact
     6661 gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga
     6721 tccggtgggg caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg
     6781 tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga
     6841 tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgcggtg gcaaagaaac
     6901 gtgtcgcact aacgctgcaa cggtggcggt gattcaggac gccctgaaat cctgcg
//
LOCUS       DAJTVJ010000383         4721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.guided.223, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000383 DAJTVJ010000000
VERSION     DAJTVJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.guided.223"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(150..1127)
                     /gene="intI2"
                     /locus_tag="OO448_004648"
                     /pseudo
     CDS             complement(150..1127)
                     /gene="intI2"
                     /locus_tag="OO448_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1459..1932
                     /locus_tag="OO448_004649"
     CDS             1459..1932
                     /locus_tag="OO448_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6339603.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2027..2551
                     /gene="sat2"
                     /locus_tag="OO448_004650"
     CDS             2027..2551
                     /gene="sat2"
                     /locus_tag="OO448_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6339604.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2609..3397
                     /gene="aadA1"
                     /locus_tag="OO448_004651"
     CDS             2609..3397
                     /gene="aadA1"
                     /locus_tag="OO448_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6339605.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            3473..3970
                     /locus_tag="OO448_004652"
     CDS             3473..3970
                     /locus_tag="OO448_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339606.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4031..4402
                     /locus_tag="OO448_004653"
     CDS             4031..4402
                     /locus_tag="OO448_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339607.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT         1299 a         1064 c         1148 g         1210 t
ORIGIN      
        1 atggtcactg tgattgacag tatcttgctt aaacttatct gaacaatggt agaactggct
       61 catatttatc ccctctatga ttgtttatct ttttcaccat aaaaagtatc aggggctact
      121 cagggctaca taaagatatt tatctacttt tactgattga taagtagcat cagtccatcc
      181 gcaggactgg tggtgccggc aaaatgctga cccaacacat gcgtatagat ttgcgtggtc
      241 ttaacatcgt tatgccctaa gagttcttgc acagtgcgaa tatcacgccc cgcttgtaat
      301 agatgcgtag caaacgagtg acgaaatgta tgacaagtga cacgcttgct aacgatgcct
      361 gctttttgta cggctgcctt caatgccttt cgcgcaacgg agtcatgcag atgatggcgg
      421 cataatttgc cgttatacgg gtggttgcag agcgtgctgg agggaaagac aaacatccac
      481 gccgcttgtc gataagcaga agggtatttg tgatctaaag caaaaggcag cgatggccct
      541 acgccttgta agttgtcgtc ttgctgaata agccgcgctt gctcaatgag ttattttatt
      601 gctgggatta ggcgcgtggg cagtaggctg tttctgcttt tcccaccctt accgtcatgc
      661 acagtgatgc agccattatc aaaatcaaaa tctttaaccc gcaaacgcaa gcattcatta
      721 atgcgcaaac ctgcaccata cagcagcgta aaaataactt ggttgcgagt atccataacc
      781 tgcaaaatgc gttgcacttc atttgcagag ataacagagg gtagccgtct aggcttgctt
      841 gcagggatat aatcaatatc gcccaacggc tgttgtaaaa acctgttgta caaaaaagct
      901 agggcattta aagcgatttt ctgcgtgttt atggctacat gtctgctgtt tgctaagctg
      961 gataaaaaca gcctgacctc ttcactgccc atggtctgag gatgacgttt tttgtgaaac
     1021 agaataaaac gcttaatcca gtgcaggtaa gttttttcag ttttcagcgc ataacctttt
     1081 tgtcgcatat ccgtgcgtat agaatttaaa aatggactgt tagacataaa acgctccttg
     1141 tcttgcaact gtctgcctat acagcctatt ctagctggga tttaaaaaag tgcctgtttt
     1201 ttacgcctag agatgcttgt ttaccggtag agttttaatt taatgctaaa taaattaaaa
     1261 tgttatgagt tctttgggtg agataatgtg catcgtgcaa gcaggataga cggcatgcac
     1321 gatttgtaat aacagagtgt cttgtatttt taaagaaagt ctatttaata caagtgatta
     1381 tattaattaa cggtaagcat cagcgggtga caaaacgagc atgcttacta ataaaatgtt
     1441 aacctctgag gaagaattgt gaaactatca ctaatggtag ctatatcgaa gaatggagtt
     1501 atcgggaatg gccctgatat tccatggagt gccaaaggtg aacagctcct gtttaaagct
     1561 attacctata accaatggct gttggttgga cgcaagactt ttgaatcaat gggagcatta
     1621 cccaaccgaa agtatgcggt cgtaacacgt tcaagtttta catctgacaa tgagaacgta
     1681 ttgatctttc catcaattaa agatgcttta accaacctaa agaaaataac ggatcatgtc
     1741 attgtttcag gtggtgggga gatatacaaa agcctgatcg atcaagtaga tacactacat
     1801 atatctacaa tagacatcga gccggaaggt gatgtttact ttcctgaaat ccccagcaat
     1861 tttaggccag tttttaccca agacttcgcc tctaacataa attatagtta ccaaatctgg
     1921 caaaagggtt aacaagtggc agcaacggat tcgcaaacct gtcacgcctt ttgtaccaaa
     1981 agccgcgcca ggtttgcgat ccgctgtgcc aggcgttagg cgtcatatga agatttcggt
     2041 gatccctgag caggtggcgg aaacattgga tgctgagaac catttcattg ttcgtgaagt
     2101 gttcgatgtg cacctatccg accaaggctt tgaactatct accagaagtg tgagccccta
     2161 ccggaaggat tacatctcgg atgatgactc tgatgaagac tctgcttgct atggcgcatt
     2221 catcgaccaa gagcttgtcg ggaagattga actcaactca acatggaacg atctagcctc
     2281 tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc acagtctcat
     2341 cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat tagagacaca
     2401 aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc tcggcggcat
     2461 tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga tgtactggta
     2521 ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca ccgcttcgcg
     2581 gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac
     2641 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     2701 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     2761 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     2821 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     2881 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     2941 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     3001 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     3061 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     3121 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     3181 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     3241 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta ggcaggctta
     3301 tcttggacaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgttcacta
     3361 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg
     3421 acgccgcttc gcggcgcggc ttaactcaag cgttagagag ctggggaaga ctatgcgcga
     3481 tctgttgaag gtggttctaa gcctcgtact tgcgatggca tcggggcagg cacttgctga
     3541 cctgccaatt gttttagtgg atgaagctcg tcttccctat gactactccc catccaacta
     3601 cgacatttct ccaagcaact acgacaactc cataagcaat tacgacaata gtccatcaaa
     3661 ttacgacaac tctgagagca actacgataa tagttcatcc aattacgaca atagtcgcaa
     3721 cggaaatcgt aggcttatat atagcgcaaa tgggtctcgc actttcgccg gctactacgt
     3781 cattgccaac aatgggacaa cgaacttctt ttccacatct ggcaaaagga tgttctacac
     3841 cccaaaaggg gggcgcggcg tctatggcgg caaagatggg agcttctgcg gggcattggt
     3901 cgtcataaat ggccaatttt cgcttgccct gacagataac ggcctgaaga tcatgtatct
     3961 aagcaactag cctgctctct aataaaatgt taggcctcaa catctagtcg caagctgagg
     4021 ggaaccacta gtgtcatacg aacctccaag agacggttac acaaacgggt acattgttga
     4081 tgtcatgtat gacaatcgcc caagtaagta tccagctgtg ttcagaacgt acgtccgaat
     4141 tccagactat gtcgaactag ataacgcgga ggagctggcc gacgcaatca ttcgagaggt
     4201 tgagttaaag gaggcatggc tcacaagggt cttggggaac tcagccgatc ctgacgcttc
     4261 tgtgggcaag tgggaccggc gcctagcgca gtgcggtggt attgatgaga tgtcaagtgg
     4321 ggtgcaggca acatacttca gtccaaacgg tcagctggtc ggcttagtgg catacattga
     4381 tgccgaatgg atggatgaat agctctgacg ttacgattca ggtgagacaa ccagatgggc
     4441 attgcgtaca agctcagttg ccaccgatgt ggctacgagt ctgatcttct ttacctaggc
     4501 caaggtatgg cgatgcttcc tgagcaaata gtcggaacgt gcacatgctg cgacaatcta
     4561 acgacgattt ctataaatga aagctacgga gtatgctcac tttgtggggc taaggatcgt
     4621 gtgatattta caagctatca gcatgaggca aggccaagaa atccctgcta tctagagaga
     4681 agtagactgg ccccctgaat ctccagacaa ccagtatcac t
//
LOCUS       DAJTVJ010000384         4250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.guided.235, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000384 DAJTVJ010000000
VERSION     DAJTVJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.guided.235"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..508
                     /gene="frdB"
                     /locus_tag="OO448_004654"
     CDS             <1..508
                     /gene="frdB"
                     /locus_tag="OO448_004654"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6339608.1"
                     /translation="ACKTFLRDYTDGMKVEALANFPIERDLVVDMTHFIESLEAIKPY
                     IIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAH
                     RYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDF
                     LIATLKPR"
     gene            519..914
                     /gene="frdC"
                     /locus_tag="OO448_004655"
     CDS             519..914
                     /gene="frdC"
                     /locus_tag="OO448_004655"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6339609.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            925..1284
                     /gene="frdD"
                     /locus_tag="OO448_004656"
     CDS             925..1284
                     /gene="frdD"
                     /locus_tag="OO448_004656"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6339610.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            1347..2480
                     /locus_tag="OO448_004657"
     CDS             1347..2480
                     /locus_tag="OO448_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6339611.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2569..3102
                     /gene="blc"
                     /locus_tag="OO448_004658"
     CDS             2569..3102
                     /gene="blc"
                     /locus_tag="OO448_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6339612.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3099..3416)
                     /gene="sugE"
                     /locus_tag="OO448_004659"
     CDS             complement(3099..3416)
                     /gene="sugE"
                     /locus_tag="OO448_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6339613.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3591..3737)
                     /gene="ecnB"
                     /locus_tag="OO448_004660"
     CDS             complement(3591..3737)
                     /gene="ecnB"
                     /locus_tag="OO448_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6339614.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3848..3973)
                     /gene="ecnA"
                     /locus_tag="OO448_004661"
     CDS             complement(3848..3973)
                     /gene="ecnA"
                     /locus_tag="OO448_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6339615.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(4025..>4250)
                     /locus_tag="OO448_004662"
     CDS             complement(4025..>4250)
                     /locus_tag="OO448_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6339616.1"
                     /translation="VTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLST
                     GAVVKVPLFVQIGEVIKVDTRSGEYVSRVK"
BASE COUNT         1064 a         1090 c         1090 g         1006 t
ORIGIN      
        1 ggcatgtaaa accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa
       61 cttcccgatt gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc
      121 gatcaaaccg tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac
      181 cccggcgcag atggcaaagt atcaccagtt ctccggttgc atcaactgtg gtctgtgcta
      241 cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct
      301 ggcgcatcgt tataacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt
      361 gaacagccag aacggcgtat ggagctgtac tttcgtgggc tattgctccg aagtctgccc
      421 gaaacacgtc gatccggctg cggccattca gcagggcaaa gtagaaagtt cgaaagactt
      481 tcttatcgcg accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt
      541 atgtacggcc aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttttacatgc
      601 tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt
      661 ttgccctgaa aaatggcccg gaagcctggg cgggattcgt cgacttttta caaaacccgg
      721 ttatcgtgat cattaacctg atcactctgg cggcagctct gctgcacacc aaaacctggt
      781 ttgaactggc accgaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc
      841 caattatcaa aagtctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg
      901 ccctgtactg gtaaggagcc tgagatgatt aatccaaatc caaagcgttc tgacgaaccg
      961 gtattctggg gcctcttcgg ggccggtggt atgtggagcg caattattgc gccggtgatg
     1021 atcctgctgg tgggtattct gctgccactg gggctgtttc cgggtgatgc gctgagctac
     1081 gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc
     1141 gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga tctgaaaatt
     1201 cacgtgcctg cgggcaaatg ggttttctac ggtctggctg ctatcctgac agttgtcacg
     1261 ctgattggta tcgttacaat ctaacgtatc gccaatgtaa atccggcccg cctatggcgg
     1321 gccgttttgt atggaaacca gaccttatgt tcaaaacgac gctctgcgcc ttattaatta
     1381 ccgcctcttg ctccacattt gctgcccctc aacaaatcaa cgatattgtg catcgcacaa
     1441 ttaccccgct tatagagcaa caaaagatcc ccggtatggc ggtggcggta atttatcagg
     1501 gtaaacctta ttactttacc tggggctatg cggacatcgc caaaaagcag cccgtcacac
     1561 agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg
     1621 acgctattgc tcgaggggaa atcaagttaa gcgatcccgc aacaaaatac tggcctgaac
     1681 ttactgctaa acagtggaat gggatcacac tattacatct tgcgacctac accgctggcg
     1741 gcctgccatt gcaggtgccg gatgaagtga aatcctcaag cgacttgctg cgcttctatc
     1801 aaaactggca gcctgcatgg gctccaggaa cacaacgtct gtatgccaac tccagtatcg
     1861 gtttgttcgg cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa
     1921 ctcgtgtctt ccagccactc aaactcaacc atacgtggat taatgtacct cccccagaag
     1981 aaaagaatta cgcctgggga tatcgcgaag gtaaggcagt gcatgtttcg ccaggggcgt
     2041 tagatgctga agcttatggt gtgaagtcga ccattgaaga tatggcccgc tgggtgcaaa
     2101 gcaatttaac cccccttgat atcaatgaga aaacgcttca acaagggata caactggcac
     2161 aatctcgcta ctggcaaacc ggcgatatgt atcagggcct gggctgggaa atgctggact
     2221 ggccggtcaa tcctgacagc atcattaacg gcagtgacaa taaaattgca ctggcagcac
     2281 gcccggtaaa agcgattaag cccccaactc ctgcagtacg cgcatcatgg gtacataaaa
     2341 cgggggcgac cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta
     2401 tcgtgatgct ggctaacaaa aactatccca atccagcgag agtcgccgcc gcctgtcaga
     2461 ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt
     2521 ttccttgctg tcatctacac ttagaaaaaa accagtaagg aaacatttat gcgcctgctc
     2581 cctctcgttg ccgcagcgac agctgcattt ctggtcgttg cctgcagttc tcctacgccg
     2641 ccgcgtggcg tgacagtagt gaataatttc gacgctaaac gctatctcgg tacctggtat
     2701 gagattgccc gttttgatca ccgttttgaa cgtggactgg aaaaagtcac cgcaacatac
     2761 agtctgcgtg atgacggcgg cctgaatgtc atcaataaag gctataaccc tgacagagaa
     2821 atgtggcagc agagtgaagg gaaagcgtac tttaccggcg caccaacacg cgctgcgctg
     2881 aaagtgtcat tctttggtcc tttctatggc ggttataacg tcattgcact tgatcgggaa
     2941 taccgccatg cgctggtttg cggcccggac cgcgactacc tgtggatact ctcccgcacg
     3001 ccaaccattt ctgacgaagt gaaacaggag atgctggcag tcgcgacccg ggaagggttt
     3061 gatgtcagta agtttatttg ggtacagcag cccggtagtt agtgagtgct gagtttcaga
     3121 ccaataatcc ccaatacgat taacgccaga ctcgccaggc gcatcgggtt agcggactca
     3181 ccgagcagca caatgccggt gatggccgcg ccgactgcgc caatacccgt ccacacggca
     3241 taagccgtcc ctaccggtaa cgatttcatc gcccaggcaa gtagcgccat actgactatc
     3301 atcgccgtca cagtaataac acttggcgtc aaacgactaa agccgtgggt atatttcagg
     3361 ccaacggccc ataccacttc cagcagacca gcaataacta agataatcca ggacatatca
     3421 ggctccggag aattggggcc atccccggtg aaaagaggcg tttgcaggtc gtcctgcaaa
     3481 gctgatggtt gaacggatat tttgccaggt ggcaggggaa tttcaactat tacctttgtg
     3541 actatcgcaa aaaaacaaac ggcacgacat aactgtcgta ccgttattgc ttattgctgc
     3601 gctttcgttg ctgcaccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
     3661 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
     3721 attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc
     3781 aaattaataa tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaagaga
     3841 agaaaaatta gctggcagcg cgggagatgg agttgccgag atgtttgatg tcttcgccga
     3901 aaccgcgagc ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca agaacgataa
     3961 ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac
     4021 cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc
     4081 cgatttgtac gaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc
     4141 cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa
     4201 cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg
//
LOCUS       DAJTVJ010000385         3939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.guided.248, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000385 DAJTVJ010000000
VERSION     DAJTVJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.guided.248"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..252)
                     /gene="tetR(A)"
                     /locus_tag="OO448_004663"
     CDS             complement(<1..252)
                     /gene="tetR(A)"
                     /locus_tag="OO448_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6339617.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNAR"
     gene            358..1557
                     /gene="tet(A)"
                     /locus_tag="OO448_004664"
     CDS             358..1557
                     /gene="tet(A)"
                     /locus_tag="OO448_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6339618.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(1524..1712)
                     /locus_tag="OO448_004665"
                     /pseudo
     CDS             complement(1524..1712)
                     /locus_tag="OO448_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1756..2145)
                     /locus_tag="OO448_004666"
     CDS             complement(1756..2145)
                     /locus_tag="OO448_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339619.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2363..2803
                     /locus_tag="OO448_004667"
     CDS             2363..2803
                     /locus_tag="OO448_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6339620.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(3559..3708)
                     /locus_tag="OO448_004668"
     CDS             complement(3559..3708)
                     /locus_tag="OO448_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339621.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT          918 a         1054 c         1050 g          917 t
ORIGIN      
        1 gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt
       61 atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa
      121 gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag
      181 accgtctacg ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg
      241 caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac caacttatca
      301 gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg
      361 aaacccaaca gacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc
      421 ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc
      481 accgcccact atggcattct gctggcgctg tatgcgttga tgcaatttgc ctgcgcacct
      541 gtgctgggcg cgctgtcgga tcgtttcggg cggcggccgg tcttgctcgt ctcgctggcc
      601 ggcgctgctg tcgactacgc catcatggcg acggcgcctt tcctttgggt tctctatatc
      661 gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc
      721 gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg
      781 ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct
      841 ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg
      901 ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct
      961 tcgttccggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc
     1021 atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt
     1081 cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc
     1141 gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg
     1201 ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg
     1261 atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa
     1321 gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg
     1381 gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct
     1441 tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc
     1501 ctgccggcgc tgcgtcgcgg gctttggaga aattcttcaa attcccgttg cacatagccc
     1561 ggcaattcct ttccctgctc tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg
     1621 atctgataga gaagggtttg ctcgggtcgg tggctctggt aacgaccagt atcccgatcc
     1681 cggctggccg tcctggccgc cacatgaggc atgttccgcg tccttgcaat actgtgttta
     1741 catacagtct atcgcttagc ggaaagttct tttaccctca gccgaaatgc ctgccgttgc
     1801 tagacattgc cagccagtgc ccgtcactcc cactcttaca ttgttcgcag tatctaggta
     1861 catgttattg cgtgcacctt catgatgtat aatgttatta atgacagcag aaagatcgac
     1921 agaattaata ttcttcgtac ctgggacagt tatcgggtgt cggccagtga tgagaccaaa
     1981 ggatgcagta taagatgaac cactactatt atcatggcca ccattaccac caccgttaaa
     2041 actacctccc gaacttccag agccgctatg accatcattg taggggtcat ttggtcccat
     2101 gtgaccaaaa ccaccgacag taccgctatt taactctgta ctcataaaat tatcctcttt
     2161 gacttttaaa aacagcaaaa actgtattta taacaatgtg tttatataca gtattcgaaa
     2221 tactacgccc gtcaaaacca caccgtcaac aggttttttg tgtatttttc acgcctcacg
     2281 ggcagcctca gaccttttgt gttaaaacag taatatgttc tgctgttaca atacggtaca
     2341 cacaggagac actgtcactt ttatggcatg gcacacagtt caagagacac tgaaattaac
     2401 aggaaagtcg cgctcgcaat tatatcgtga catggctaaa ggccttgtca gctatcgcac
     2461 aggaaaaaat gatcgccggg agttcgagac cagtgaactg attcgggcct atggcgagct
     2521 gcgcacttct gagacaccaa aaaggcacaa ggagggacac tccaaatctg tcgttagcca
     2581 ggcggaaaat gctcagctag acgtcattca gcagaaactg gacgctctgc agcagactgt
     2641 taccctaatg cttgaagaca agatggcgcg ggaagcagaa aaccgcctgc gaaatgagga
     2701 gagggaacaa ctacaggccg aaattagccg tctggctgac gcgctggcac aggaaaaaaa
     2761 gcggggattc tggtcgcggc tgtttcgctc cggttcagag taacagtgca ggtgactctg
     2821 atttaccata acatctgtta tcagcaccgc ctgagaggcc ctcagggcgt ttctgacggg
     2881 aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt
     2941 tttacggcat gcatgagtat gcgcacaagt gcatagcgcg gaaattcaca ccattcagcc
     3001 ggggtaagtc ctgtttccgg cgttcttccc atccgaaaac ggtgaacggg gacgcaattt
     3061 attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac ccgctgtcag cacgttcagg
     3121 aagggggtga tgtacaaatg gcacagagga tgaaagtgac gtatgagctg aaggaatgat
     3181 gtaaaagtga tgtataagtg atgtataagt gatgtaaaag tgatgtatga actgaaggag
     3241 cgatgtaatt ctgtagtcag gaagaataac ccgttcatgc aggcatgaac gatacagtga
     3301 ggaacgcttt ttttgtcacg atctcccctt aacctgcgta aatcacccgc catggttgat
     3361 tatttttcaa cctcaaaatg gtcatacacc taacgcagcc gcgattttca tcgcaactgc
     3421 atgaattaaa agaaaaaaaa ggaatatttc gttttcacga gtaatacccg ggtattaaaa
     3481 atccactgta attacatgaa aataaagaaa taaaaggtaa taccggagta ttaccacgta
     3541 cgcgggcaag cgaaaaagct ataacgcttc ccggccgaca ggcgacccaa aatccacagg
     3601 ggcatgcagg ttctcttcag taacctgcgc cattaaatca aaaaggtcat attcgccggg
     3661 aagctgcgta tcagtcgaat ccagttgttc atcatattca gatggcatgg ggtgatatcc
     3721 agtagctgag aggttgttag cgggtttcgg ggcgcagccc tgaaccagtc atgtagcgct
     3781 agcggagtgt atactggctt actatgttgc cacgattgcg aaaatcagtg aagtgcttca
     3841 tgtggcagga gaaaaaaggc agcaccggcg cgtcagcaga atatgcgata caggagatat
     3901 tccgcttcct cgctcactga ctcgctacgc tcggtcgtt
//
LOCUS       DAJTVJ010000386         2309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.guided.295, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000386 DAJTVJ010000000
VERSION     DAJTVJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.guided.295"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..252)
                     /gene="tetR(A)"
                     /locus_tag="OO448_004669"
     CDS             complement(<1..252)
                     /gene="tetR(A)"
                     /locus_tag="OO448_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6339622.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNAR"
     gene            358..1557
                     /gene="tet(A)"
                     /locus_tag="OO448_004670"
     CDS             358..1557
                     /gene="tet(A)"
                     /locus_tag="OO448_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6339623.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1589..2287)
                     /locus_tag="OO448_004671"
     CDS             complement(1589..2287)
                     /locus_tag="OO448_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCR6339624.1"
                     /translation="MRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFI
                     SAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTR
                     KWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRS
                     NRTPRARIACRKSP"
BASE COUNT          434 a          719 c          699 g          457 t
ORIGIN      
        1 gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt
       61 atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa
      121 gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag
      181 accgtctacg ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg
      241 caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac caacttatca
      301 gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg
      361 aaacccaaca gacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc
      421 ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc
      481 accgcccact atggcattct gctggcgctg tatgcgttga tgcaatttgc ctgcgcacct
      541 gtgctgggcg cgctgtcgga tcgtttcggg cggcggccgg tcttgctcgt ctcgctggcc
      601 ggcgctgctg tcgactacgc catcatggcg acggcgcctt tcctttgggt tctctatatc
      661 gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc
      721 gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg
      781 ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct
      841 ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg
      901 ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct
      961 tcgttccggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc
     1021 atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt
     1081 cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc
     1141 gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg
     1201 ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg
     1261 atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa
     1321 gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg
     1381 gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct
     1441 tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc
     1501 ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg
     1561 tggaaacgat aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc
     1621 taggagtgcg gttggaacgt tggcccagcc agatactccc gatcacgagc aggacgccga
     1681 tgatttgaag cgcactcagc gtctgatcca agaacaacca tcctagcaac acggcggtcc
     1741 ccgggctgag aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga
     1801 accaaaggaa gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat
     1861 tggttcctgt aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa
     1921 gtcctccggc cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact
     1981 tgcgggtcag cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc
     2041 cgacaggatc tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc
     2101 aaatagcccc caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga
     2161 acacgaccat cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt
     2221 agaccgaaat aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga
     2281 tgcgcatcca ccagattccc gttggaatc
//
LOCUS       DAJTVJ010000387         2094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.guided.308, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000387 DAJTVJ010000000
VERSION     DAJTVJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.guided.308"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            99..902
                     /locus_tag="OO448_004672"
                     /note="aph(3'')-Ib"
     CDS             99..902
                     /locus_tag="OO448_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6339625.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            902..1738
                     /locus_tag="OO448_004673"
     CDS             902..1738
                     /locus_tag="OO448_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6339626.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          443 a          575 c          604 g          472 t
ORIGIN      
        1 tattggcggc gctgaaagaa accgcaagaa ttcgttaact gcacattcgg gatatttctc
       61 tatattcgcg cttcatcaga aaactgaagg aacctccatt gaatcgaact aatatttttt
      121 ttggtgaatc gcattctgac tggttgcctg tcagaggcgg agaatctggt gattttgttt
      181 ttcgacgtgg tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc
      241 tcgctggaga gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg
      301 tcatcaactg gcaggaggaa caggagggtg catgcttggt gataacggca attccgggag
      361 taccggcggc tgatctgtct ggagcggatt tgctcaaagc gtggccgtca atggggcagc
      421 aacttggcgc tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc
      481 gaatgttcgg acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac
      541 cggacgagga caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac
      601 cggtgcggct cgaccaagag cgcaccgata tggttgtttg ccatggtgat ccctgcatgc
      661 cgaacttcat ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc
      721 tcggaacagc agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact
      781 gggcagcgcc agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg
      841 aagcccccga ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt
      901 gatgttcatg ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc
      961 ggacaccttt tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt
     1021 caagggattg aaacctatag aagacattgc tgatgaactg cgcggggccg actatctggt
     1081 atggcgcaat gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct
     1141 cgaatatgcc ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc
     1201 gaccgaaatt gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc
     1261 ttctgccctt ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga
     1321 tcaaaacgca ggttgtcaaa ctgactacgt ccacgcggcg attatagccg atcaaatgat
     1381 gagcaatgcc tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt
     1441 ctccagtcgc ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg
     1501 cgccgccaat atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg
     1561 cattgcacag atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct
     1621 cgaccaggcg tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga
     1681 gcaacgcgat ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat
     1741 atcaagcgac ttctcctatc ccctgggaac acatcaatct caccggagaa tatcgctggc
     1801 caaagcctta gcgtaggatt ccgccccttc ccgcaaacga cccctggaag ccccgtagcg
     1861 acgcggagag gggcgagaca agccaagggc gcaggctcga tgcgcagcac gacatagccg
     1921 gttctcgcaa ggacgagaat ttccctgcgg tgcccctcaa gtgtcaatga aagtttccaa
     1981 cgcgagccat tcgcgagagc cttgagtcca cgctagatct atctcatctg cgcaaggcag
     2041 aacgtgaaga cggccgccct ggacctcgcc cgcgagcgcc aggcgcacga ggcc
//
LOCUS       DAJTVJ010000388         1813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466782-rid15818283.guided.315, whole genome shotgun
            sequence.
ACCESSION   DAJTVJ010000388 DAJTVJ010000000
VERSION     DAJTVJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466782
            Sequence Read Archive: ERR10074648
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477314.1
            Genome Coverage       :: 77x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:36:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,674
            CDSs (total)                      :: 4,585
            Genes (coding)                    :: 4,288
            CDSs (with protein)               :: 4,288
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 134 of 297
            Pseudo Genes (incomplete)         :: 101 of 297
            Pseudo Genes (internal stop)      :: 98 of 297
            Pseudo Genes (multiple problems)  :: 34 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466782-rid15818283.guided.315"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2013-04-11"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="OO448_004674"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="OO448_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6339627.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          402 a          521 c          472 g          418 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta act
//
